LOCUS       DAJSMI010000001        94874 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJSMI010000001 DAJSMI010000000
VERSION     DAJSMI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 94874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.001"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(27..737)
                     /locus_tag="ON430_000001"
     CDS             complement(27..737)
                     /locus_tag="ON430_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCR3217954.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(734..2206)
                     /locus_tag="ON430_000002"
     CDS             complement(734..2206)
                     /locus_tag="ON430_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3217955.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2344..2817
                     /locus_tag="ON430_000003"
     CDS             2344..2817
                     /locus_tag="ON430_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3217956.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2970..3521)
                     /locus_tag="ON430_000004"
     CDS             complement(2970..3521)
                     /locus_tag="ON430_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3217957.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3626..4156
                     /locus_tag="ON430_000005"
     CDS             3626..4156
                     /locus_tag="ON430_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCR3217958.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4639..5082)
                     /locus_tag="ON430_000006"
     CDS             complement(4639..5082)
                     /locus_tag="ON430_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3217959.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5098..6258)
                     /locus_tag="ON430_000007"
     CDS             complement(5098..6258)
                     /locus_tag="ON430_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3217960.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6765..8465
                     /locus_tag="ON430_000008"
     CDS             6765..8465
                     /locus_tag="ON430_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3217961.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8567..9400)
                     /locus_tag="ON430_000009"
     CDS             complement(8567..9400)
                     /locus_tag="ON430_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR3217962.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9704..11869)
                     /locus_tag="ON430_000010"
     CDS             complement(9704..11869)
                     /locus_tag="ON430_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3217963.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12129..14423
                     /locus_tag="ON430_000011"
     CDS             12129..14423
                     /locus_tag="ON430_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR3217964.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14416..15081
                     /gene="pgmB"
                     /locus_tag="ON430_000012"
     CDS             14416..15081
                     /gene="pgmB"
                     /locus_tag="ON430_000012"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR3217965.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15074..16081
                     /locus_tag="ON430_000013"
     CDS             15074..16081
                     /locus_tag="ON430_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3217966.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16103..17122
                     /locus_tag="ON430_000014"
     CDS             16103..17122
                     /locus_tag="ON430_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3217967.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17403..18770)
                     /gene="nhaC"
                     /locus_tag="ON430_000015"
     CDS             complement(17403..18770)
                     /gene="nhaC"
                     /locus_tag="ON430_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR3217968.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18879..20300)
                     /locus_tag="ON430_000016"
     CDS             complement(18879..20300)
                     /locus_tag="ON430_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3217969.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20543..22420)
                     /gene="tdc"
                     /locus_tag="ON430_000017"
     CDS             complement(20543..22420)
                     /gene="tdc"
                     /locus_tag="ON430_000017"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR3217970.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22711..23967)
                     /locus_tag="ON430_000018"
     CDS             complement(22711..23967)
                     /locus_tag="ON430_000018"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR3217971.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24079..24284)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24547..25848
                     /locus_tag="ON430_000019"
     CDS             24547..25848
                     /locus_tag="ON430_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR3217972.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25917..26516)
                     /locus_tag="ON430_000020"
     CDS             complement(25917..26516)
                     /locus_tag="ON430_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3217973.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26770..27651
                     /locus_tag="ON430_000021"
     CDS             26770..27651
                     /locus_tag="ON430_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3217974.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27664..29274
                     /locus_tag="ON430_000022"
     CDS             27664..29274
                     /locus_tag="ON430_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCR3217975.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29351..30010)
                     /locus_tag="ON430_000023"
     CDS             complement(29351..30010)
                     /locus_tag="ON430_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3217976.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30293..30958)
                     /locus_tag="ON430_000024"
     CDS             complement(30293..30958)
                     /locus_tag="ON430_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCR3217977.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(31105..31710)
                     /gene="dhaL"
                     /locus_tag="ON430_000025"
     CDS             complement(31105..31710)
                     /gene="dhaL"
                     /locus_tag="ON430_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCR3217978.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(31738..32748)
                     /gene="dhaK"
                     /locus_tag="ON430_000026"
     CDS             complement(31738..32748)
                     /gene="dhaK"
                     /locus_tag="ON430_000026"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR3217979.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(32723..33169)
                     /gene="dhaM"
                     /locus_tag="ON430_000027"
     CDS             complement(32723..33169)
                     /gene="dhaM"
                     /locus_tag="ON430_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCR3217980.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            33272..33592
                     /locus_tag="ON430_000028"
     CDS             33272..33592
                     /locus_tag="ON430_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR3217981.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(33704..34573)
                     /locus_tag="ON430_000029"
     CDS             complement(33704..34573)
                     /locus_tag="ON430_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3217982.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(34669..35973)
                     /locus_tag="ON430_000030"
     CDS             complement(34669..35973)
                     /locus_tag="ON430_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3217983.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(35970..36479)
                     /locus_tag="ON430_000031"
     CDS             complement(35970..36479)
                     /locus_tag="ON430_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3217984.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(36654..38789)
                     /locus_tag="ON430_000032"
     CDS             complement(36654..38789)
                     /locus_tag="ON430_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3217985.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(38872..40527)
                     /locus_tag="ON430_000033"
     CDS             complement(38872..40527)
                     /locus_tag="ON430_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3217986.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(40529..40843)
                     /locus_tag="ON430_000034"
     CDS             complement(40529..40843)
                     /locus_tag="ON430_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3217987.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            41013..41273
                     /locus_tag="ON430_000035"
     CDS             41013..41273
                     /locus_tag="ON430_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCR3217988.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            41287..41601
                     /locus_tag="ON430_000036"
     CDS             41287..41601
                     /locus_tag="ON430_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCR3217989.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(42331..42885)
                     /locus_tag="ON430_000037"
     CDS             complement(42331..42885)
                     /locus_tag="ON430_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR3217990.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(43011..44940)
                     /locus_tag="ON430_000038"
                     /pseudo
     CDS             complement(43011..44940)
                     /locus_tag="ON430_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(45246..45962)
                     /locus_tag="ON430_000039"
     CDS             complement(45246..45962)
                     /locus_tag="ON430_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR3217991.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(45963..47786)
                     /gene="glpO"
                     /locus_tag="ON430_000040"
     CDS             complement(45963..47786)
                     /gene="glpO"
                     /locus_tag="ON430_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCR3217992.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(47800..49296)
                     /gene="glpK"
                     /locus_tag="ON430_000041"
     CDS             complement(47800..49296)
                     /gene="glpK"
                     /locus_tag="ON430_000041"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR3217993.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(49432..50892)
                     /locus_tag="ON430_000042"
     CDS             complement(49432..50892)
                     /locus_tag="ON430_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3217994.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(50882..52177)
                     /locus_tag="ON430_000043"
     CDS             complement(50882..52177)
                     /locus_tag="ON430_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3217995.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(52509..53186)
                     /locus_tag="ON430_000044"
     CDS             complement(52509..53186)
                     /locus_tag="ON430_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCR3217996.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(53216..54307)
                     /locus_tag="ON430_000045"
     CDS             complement(53216..54307)
                     /locus_tag="ON430_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCR3217997.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(54408..54842)
                     /locus_tag="ON430_000046"
     CDS             complement(54408..54842)
                     /locus_tag="ON430_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3217998.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(55426..56883)
                     /gene="ascB"
                     /locus_tag="ON430_000047"
     CDS             complement(55426..56883)
                     /gene="ascB"
                     /locus_tag="ON430_000047"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3217999.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(56880..58775)
                     /locus_tag="ON430_000048"
     CDS             complement(56880..58775)
                     /locus_tag="ON430_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3218000.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(58833..60608)
                     /locus_tag="ON430_000049"
     CDS             complement(58833..60608)
                     /locus_tag="ON430_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR3218001.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(60658..62154)
                     /locus_tag="ON430_000050"
     CDS             complement(60658..62154)
                     /locus_tag="ON430_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3218002.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(62348..63970)
                     /locus_tag="ON430_000051"
     CDS             complement(62348..63970)
                     /locus_tag="ON430_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3218003.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(64298..65611)
                     /locus_tag="ON430_000052"
     CDS             complement(64298..65611)
                     /locus_tag="ON430_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR3218004.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(65856..66080)
                     /locus_tag="ON430_000053"
     CDS             complement(65856..66080)
                     /locus_tag="ON430_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218005.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(66100..66522)
                     /locus_tag="ON430_000054"
     CDS             complement(66100..66522)
                     /locus_tag="ON430_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCR3218006.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(66665..67375)
                     /locus_tag="ON430_000055"
     CDS             complement(66665..67375)
                     /locus_tag="ON430_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218007.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(67393..67890)
                     /locus_tag="ON430_000056"
     CDS             complement(67393..67890)
                     /locus_tag="ON430_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3218008.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(67927..68379)
                     /locus_tag="ON430_000057"
     CDS             complement(67927..68379)
                     /locus_tag="ON430_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3218009.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(68514..69119)
                     /locus_tag="ON430_000058"
     CDS             complement(68514..69119)
                     /locus_tag="ON430_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3218010.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(69569..70189)
                     /locus_tag="ON430_000059"
     CDS             complement(69569..70189)
                     /locus_tag="ON430_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3218011.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(70191..71060)
                     /locus_tag="ON430_000060"
     CDS             complement(70191..71060)
                     /locus_tag="ON430_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCR3218012.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(71083..71490)
                     /locus_tag="ON430_000061"
     CDS             complement(71083..71490)
                     /locus_tag="ON430_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR3218013.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(71509..72798)
                     /locus_tag="ON430_000062"
     CDS             complement(71509..72798)
                     /locus_tag="ON430_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3218014.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(72825..73127)
                     /locus_tag="ON430_000063"
     CDS             complement(72825..73127)
                     /locus_tag="ON430_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3218015.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(73152..75245)
                     /locus_tag="ON430_000064"
     CDS             complement(73152..75245)
                     /locus_tag="ON430_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3218016.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(75734..76498)
                     /locus_tag="ON430_000065"
     CDS             complement(75734..76498)
                     /locus_tag="ON430_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCR3218017.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(76609..77478)
                     /gene="mdcB"
                     /locus_tag="ON430_000066"
     CDS             complement(76609..77478)
                     /gene="mdcB"
                     /locus_tag="ON430_000066"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCR3218018.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(77503..78579)
                     /locus_tag="ON430_000067"
     CDS             complement(77503..78579)
                     /locus_tag="ON430_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR3218019.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(78603..79196)
                     /locus_tag="ON430_000068"
     CDS             complement(78603..79196)
                     /locus_tag="ON430_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCR3218020.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(79200..80876)
                     /gene="mdcD"
                     /locus_tag="ON430_000069"
     CDS             complement(79200..80876)
                     /gene="mdcD"
                     /locus_tag="ON430_000069"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCR3218021.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(80866..81165)
                     /locus_tag="ON430_000070"
     CDS             complement(80866..81165)
                     /locus_tag="ON430_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCR3218022.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(81267..82913)
                     /gene="mdcA"
                     /locus_tag="ON430_000071"
     CDS             complement(81267..82913)
                     /gene="mdcA"
                     /locus_tag="ON430_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCR3218023.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(82920..83831)
                     /locus_tag="ON430_000072"
     CDS             complement(82920..83831)
                     /locus_tag="ON430_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR3218024.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            83951..84808
                     /locus_tag="ON430_000073"
     CDS             83951..84808
                     /locus_tag="ON430_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3218025.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(84995..85357)
                     /locus_tag="ON430_000074"
     CDS             complement(84995..85357)
                     /locus_tag="ON430_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR3218026.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(85546..85728)
                     /locus_tag="ON430_000075"
     CDS             complement(85546..85728)
                     /locus_tag="ON430_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218027.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            85703..87142
                     /locus_tag="ON430_000076"
     CDS             85703..87142
                     /locus_tag="ON430_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3218028.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(87149..89701)
                     /locus_tag="ON430_000077"
     CDS             complement(87149..89701)
                     /locus_tag="ON430_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCR3218029.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(89815..91146)
                     /locus_tag="ON430_000078"
     CDS             complement(89815..91146)
                     /locus_tag="ON430_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3218030.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(91112..92590)
                     /locus_tag="ON430_000079"
     CDS             complement(91112..92590)
                     /locus_tag="ON430_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3218031.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(92895..94730)
                     /gene="tdc"
                     /locus_tag="ON430_000080"
     CDS             complement(92895..94730)
                     /gene="tdc"
                     /locus_tag="ON430_000080"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR3218032.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        27495 a        20040 c        16440 g        30899 t
ORIGIN      
        1 caacctccta catggtgttc aaaatattat tcgccttttg cttctttcac taattttgcg
       61 aatgcttgtt gttggaattc agcagctaag gctttcgtac gttgttcttg ctctgctttg
      121 ctcatttttg gattttcagt cattgctttt accataccgt cttgaacata agcttttgct
      181 tcactttcca tcgtactttg ctgtttcttc actaattcag ctaacttggc tgcttggtcc
      241 gtacttaatt ctacccatgt ctctgggatt ttgaatgtat tcactcggct gtcatattcg
      301 tgattgttat cagcaatatt gaaccagaat ttatacatgt cgtttttgta ttcccaataa
      361 gtagttttcg tcactttttc agcatcgccg tctgtctttt ctactttatt tgtttcgtta
      421 tccgtaccag ttgcttttgg tttttcttgt ttttcatttg ttcggtaaag atacactttt
      481 tcttttccat tccctaaagc tttatacaaa agcatatctg ctccttggga atcagcactg
      541 gaaacaattt tttctgtttt tgtttccgtt actttttcca ttccaaagtg attggacaaa
      601 ttagcgacga tcaaacttag tgaagcgacg aagattagtc cgaaaatcaa agataaaaag
      661 gtttgccatg tttttttagc gaacacgttc gttaaagcaa aagcaagcaa gctgacaatc
      721 aaaactccaa taatcatgca ttatcccccg ctttcattag aacaggttct tttttcttca
      781 ataaaaaggc aataataaag ccaagaatcc caaagatgat cgctacgata aacgcggcat
      841 ggtaacctga cagtgtggca ttgattgcct gatctttata agctaatggc gtattatcca
      901 atacagattt gcttgggata tttgcttttg tcacattcgt caacacactg atcaaaatag
      961 ctgtaccaat cgagcttgcg atttgacgga atgtattatt taccgctgtc ccatgactga
     1021 tcaagttatt tggcaatgca ttcataccag aagtcgttac cggcatcatg accatggaga
     1081 tcccaaacat acggatcgca taaaggatca cgatatataa aactggagtc gtttttgtca
     1141 agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat ccctgtgatg gctaaacgtt
     1201 tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat catagccccc atgatcaatg
     1261 cacccggcaa caacatcaag ccagaatgga atgctgattc tccacgaata ttttgaatat
     1321 acaatggtac gaccatttct gcccctacca tcgccatatt tgttacacca gctaaaacag
     1381 ctgcaatcgt gaatgtagat gatttgaata cacgaagttc caagaatgga tgttccaaat
     1441 gtaattgacg ccatgagaac aaggcgatga aaatcacacc aacaactaag aaaccaatta
     1501 ctttcaagct tccccagccg tcatctccta cactagaaaa tccgtaaagt aaactaccga
     1561 atccaatcgt tgataaaata gcggataaaa tatcaatgct aggatttgac aatggaataa
     1621 cacttttcat taggaagaat gctaaaacca agacaagaac aacgattggc agtaccatac
     1681 caaacaagtc acgccaatgg tagttatcaa tgatataacc agataatgta gggcctaaag
     1741 caggagcaag tccaataacg atccctgaca tccccattgc tgatcctctt ttttctggcg
     1801 ggaaaataga tagcatgata ttttgcaata atggcatcga tactcccaca ccgcaagctt
     1861 ggatcaaacg accgattagt aatgtactaa aattaggtgc tgtaaagcaa gtaatcgttc
     1921 cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct tgaactgaat ttattgatca
     1981 gccatgctgt tattgggatc atgatcccgt tgactagcaa gaaaccagtc gttaaccatt
     2041 gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt aggaaaagct gtcgtcaata
     2101 aggtctgatt caaaacggta caaaatgtac caattaataa gacaacaact aaaagtgtcc
     2161 gattatatgg ttttccgtaa atatctacgg gttggttgtt actcatattt ctccttcttt
     2221 ctgaattttt tctgaaacta ctagaatact ttagtcctaa attcgctatt tgtcaaccta
     2281 cttgacacat aagttaatac ggatataatt aagggaagca agaggacaaa aggagtgaca
     2341 aaaatggttg gggcttccct taaacgtttg ttggaaaatg atcaattggt tgttggcatc
     2401 agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat taaggtattg ggaacaaaag
     2461 ggattcattc gttcgatcga agctgatgcg aattgtccta gaaaatatcg tttacctaca
     2521 gtcataaaag tagaactgat caaaaaatat ttagatgacg gatttacttt gtcaaaagct
     2581 gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg tccgtaaagt tttttcaaaa
     2641 gcaatcaaag atatcgaatt gatcgacgaa cgctatacga ttttacaaat cggtaacttc
     2701 aaagaagaga atgctaagat gtacatcatt catgacaacg agacagacga attaacttac
     2761 aagctccttc ctgtcgatga aaaagctgac tacgaacagt tgttaaaaga actctaaacg
     2821 ccactaatct cctaagtacc aagcttttta ataagtagat gctacataaa tacttgaaaa
     2881 aaggcctgtt tgaacataag aatatccgaa cggtgatctg cgtttctttc atatagaaag
     2941 gaattctcca tcactgttcg gatatttttt tatttttttc ggttactttc ttctaataca
     3001 tctgttagcc aatttgcata ttctgtttcc cgattgatcg catgcgctaa aatcaggtag
     3061 tgtccgtaat tttcatgttt ttctttttca gtaggaaaga tcgttttttt tcgctctaat
     3121 agatgaagtc gtttttcttc atgaaggtca cgctgctgct gaataatttc tactaatgcc
     3181 ggatcattca cgtctttgac gaaatacaat ttcagaacaa attcgtctcg attaaccggt
     3241 agttcattgg tgggtgtatg aatccactct gccaattgtt ccttcccttc ttctgttaat
     3301 tcataaactt tcttttccaa tttttcacct gtgatctcta cttgatgctg aatgattcct
     3361 tgctcttcca tttttgcaag ttcaggataa atctgactat gttttgcttg ccaaaattct
     3421 cctatttcat ttttgaacga ttgattcaat tcatatcctg tcatcttctt cttacttaat
     3481 aaccctaaca aaatatatcg aagtgtattt tttcttgcca tcagttttcc ctccttgaca
     3541 aatctttaaa tccattctat cataaatgag taatcgaaaa catgtagttt attacatgtt
     3601 ttaatttata aggagatgct taaacatgaa agaatttaaa gaattggaag attttattgg
     3661 aactcacttt atctatacgt atgacaatgg ttgggaatat gaatggtatg ccaaaaatga
     3721 ccatactgtt gattaccgta tccatggcgg aatggtcaaa ggccgttggg taaaagacca
     3781 agaagctgat atcgttaaat tgacggacgg cgtattcaaa atcacttgga cagagccaac
     3841 agggacagac gtggcattgg actttatgcc aaatgaaaag aaacttcacg gaatgatctt
     3901 tttccctaaa tgggttgaag aacatccaga aatcactgtt actttccaaa acgaacatat
     3961 cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat ccaaaattac ttgttcctga
     4021 atttgctacg atcacttata tgggtgatgc aggtatagac aatgaagatg ttattagcga
     4081 agaaccatac gatggaatgc cagatgatat ccgtagcgga aaatacttcg atgaaaatta
     4141 taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa tcagcgttct gacctgagta
     4201 ttagagaaag aaacggaaaa atagttcctc atgttttgcc gttttttgag ctaataccga
     4261 gggtcagctg attgaacacc gtttatcaag aggttgtgaa atcagcgtta tgctttgagt
     4321 aataagaacg ctagttagaa aatagctttt catattttct aggctagtgg gcttattacc
     4381 gagaagcagc cgtttgaaca ccgtttatca agaggttgtg aaatcagcgt tttgacctga
     4441 gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt gagctaatac
     4501 cgagggtcag ctgattgaac accgtttatc aaagattgta acaactgcat attcacacag
     4561 ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa aaaattaggc tgggagcatt
     4621 atttcgttat tctgttcatc aaaaatcagc gttttgattc catttcatca caagttcctc
     4681 ctcatttact gcttcatctt tttgctgtgt ttgaattgta aaaccttgtg aaagatagaa
     4741 atgaatagct gattgatttt tttgatagac tgtcaaagtc agtgttttat tggtttcttt
     4801 tatttttttc attagttgcg taccgattct ttgttgccgg tattcccttt tcacaaagat
     4861 tcctgcgata aactgatcaa tcaccccagc aaaaccaata atttcttgat ctatttctgc
     4921 taagtaaatc actgcatctg gtaaagcttt cctaacagat tctacttgat tctcccaata
     4981 agttgatgga atgaattcat gagaatccaa gttagcatgc agccaaatcg atagaacaga
     5041 ttccagttct ttggcagaca attttgttgt tgtgcggatc attttttctc tccttactta
     5101 ttcattgatt ttaaaataaa aaccattttt ttgaagctct tttacaagtt ttcgcccctc
     5161 aaaaaagtat atcttctctt gttttacttt tctaatacca aaagaaacag caactttagg
     5221 atcatcagct gaaacgattc gataagttcc accaggcaat tgatccagct tgactttccc
     5281 atgatggtcg ctttgtactg aaaacggcac accatttcta gtaagtgttt ttttcttatg
     5341 tttcaaaatg aatgaactac catttaccgg tttccctgac gaatcactaa taataaatga
     5401 aaaatcaagc acttttttac ttaatctata ctgatagatt agtcgtccga taaacactaa
     5461 cagtatgact acagctatta tcgttcctgc aagaataaac cattgccgca tggttctttc
     5521 ctgatttcct tttccttctt ctttttttac cgattcgtta taaggaatcc gatgtcctgt
     5581 cactagcaaa cgatgcgtgt tgatcatata cggcgtacat gtaagcaagg taactaagtc
     5641 ttctccttct gaaatcgtta gaacagacgt gtcatctggc agtaccactt gtatattttt
     5701 aacttgataa gccaatttct cccccaaaac agtcaaaata aacgtgtctc cgttttctaa
     5761 acgatctaat tcggtaaata accgtcgatt aggtaaaccg ctgtgagcag agatcactga
     5821 atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc aagacgcctg ctccattttc
     5881 aaggatccta ccattgagcc gatcataaag tgaaatattt aattggatct taggtatatt
     5941 gatacttccg atcagccatt cctctaattg gctggatgtt gtactttcat ttctgttctt
     6001 ttcattgaaa ggatcagtac caggattgaa tccttggtca gttaatcgtt ggtttttctc
     6061 ctccattttc tttcgctgca cttcaatttc agctgcggtc cgtttttgta cttgttcgat
     6121 acgtttttga tcaataaaac tatttaaaga atccacataa aatggatata aagcgatcag
     6181 taaccctacg acgatacata ttaataaaaa caaacgaaac aaaagtcgac gagactgttt
     6241 ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt ttttgttctt tcttttatta
     6301 tatgggttca ggaaaaaaat agaaaatgaa agccgatgag gttgtgaaaa agctgtcctg
     6361 catcaagtaa taagtacagc ccaaaaaaat agctactcat attttttgtt gggttgggct
     6421 tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga aaactgcgtt
     6481 atgctttgag taataagagc gctagtcaga aaatagcttc tcatattttc taggctagtg
     6541 agcttattac cgagaagcag ctgattgaac accgtttacc gagaggtcat gaaaaagcta
     6601 ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa ggagatgcaa caaaacttat
     6661 gtcgcatcct ctctccttag aactttcact tatgtttttc ttgtaaaatt cataaatatt
     6721 ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg atatatgatg aatttatcta
     6781 aagtttgcaa aggagttcga caattgaaaa aatatggccc atttttagca atttttttca
     6841 ttgctttggc atttctcatg cctcatttat tcacacataa actaattttg ggtgctgatg
     6901 ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca aattaaatat ggggatttcc
     6961 attattttcc ttccctttat ggattccagc aaagtggcag gatcgtgaat gccgtttacg
     7021 gcccattttt cgcttattta caaggattga tcgtgttgct tgcaggtagc tggtttcatt
     7081 atcagattct ttcaaatttc attctttatc ttatttcagg attttctatg ctgattttac
     7141 tgagatatgt caatgttcga aattgggtaa gtgtaccagt agccgtttta ttcatgagta
     7201 cgtattctat tcaatactgg tctatcaacc aaggattcac tagctggggg acttgttttt
     7261 atcctttatg tatgatccct attgctgatt tcgtccttca taaaaagttc cctgttatca
     7321 aagtcgctgt ctcagtggga cttatgacac agatccattt actgtcttcg atcatgttag
     7381 ctttgatgta tatccctttt tatctttatt acttttttaa ccagactgaa aaaggaaaag
     7441 cactgcttca actgattaag tcggtcttgc tttattttgt tctgacagca aatatttggg
     7501 caggattagc tattgtttac agtgggaata agattcttcc tccctttgtt aacagagaaa
     7561 tgagtcaaaa ggcgatagat ctggatggca gttattggct ccattaccct aaggcctttc
     7621 ctatattgat attatcaggt tttttgcttt ttattttctt tcaacaaaag aacaccttat
     7681 tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt tctttctact agcttgattc
     7741 catggacgac attgatcgaa aaaaatgtgc cttttattag tttgatccag tttccatttc
     7801 gatttttcgt gccatttacg ctattgcttc tactttacct tgccctttca ctgaaccagt
     7861 ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg cattcttatt ttttcagcac
     7921 agacgatcca gaacttatac cagcatctgg aaaagtggga aaatgaaaca tttgtcagtc
     7981 gacatactta tctattcgat acgccggaag aagcaaggaa aacattcttc agcaaagata
     8041 tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga ctatctgcca atctataagg
     8101 agacaaaaga caataaatac gaccggtata acgaagaaat actagaaaac gaaacaactt
     8161 ttataaaaac acatccaggc ggtcttttga caatcaagtg gaaaaacact ggggacaaaa
     8221 ctgttcatat ccctgttatc gtctacgatc ggactgtgct acagcaaaat ggaaaaacat
     8281 tgactgatta tgaagtaaca gatatcggga ccccaatcgt gaagcagcaa aaaggaatca
     8341 acgagctcac gcttcattat caaacgccaa tctatttcta tttcattctt tctcttactt
     8401 tgattggctg gtttaccttg ctatgtttat ttatttatca tagatacaag cttttacgtg
     8461 cttaggcaaa agggataaat acatccgatc aaaaaactct ccaaacggta taatcgtttg
     8521 gagagtttta ttttaatcag tttttttatg ttatatgtta tattttttat tcgttcacat
     8581 ctaattcgat ttccaccccg tagtgatcac tgatgattgg actatcgatc ccatcaaaaa
     8641 tccgttgata ttttttaacc tgcattcctt cagggacgaa aataaaatct atacgcaatt
     8701 tttctgtatt ttgttcccaa ccatctattt tcttttccac agtagctgct tttccttttt
     8761 cattcgcgac aacatacgaa tcttgaagaa gaaggcaact ttctcttaca gtataataac
     8821 cttgagagtc tactggatta ttgaaatcac ctaggaaaaa aagtggtacg cgagtttcca
     8881 aaaagtagtt ttccaagttt ttccactcaa aataaaagcc acttttttta tctgtccacc
     8941 aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc tatctttgtt ttcccaatca
     9001 agatcttgcg tgtctgataa tcttctttct tttgaaattg ggagacggta aggacctgac
     9061 tttccaatgt ttttttcgta agcagtgcat ttccttcttc atatttatca taaccgatgt
     9121 ggctcatttc ccaactccaa aaatactctt ttccgtgttc ctttaaaagc tgaactaaac
     9181 ggtaggcaaa attatcttta cggataggta cttgattctt taccggacag aacttcatta
     9241 gcttcgcagg ttctacttct tcagattcca gtaattggtt gatttcttgt aaagcaatca
     9301 cttcataatc ttctaacagg atcttatctg ctaattcttg caattttttc tctggttctg
     9361 gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat tggttttctc cttgtcttca
     9421 tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac ctctccagca aatagtgttc
     9481 tcctgctaac tcctcagtaa taagctcaat ggaccaggag ctattttttg tttggctgtt
     9541 tttatcactt ggtgcagggc agctttttca cgacctctcc actaaacagt attcaaactg
     9601 ctgcttctcg gtaataagcc caaccaaacg aaaaatagga ggagctattt ttgtttggct
     9661 gttcttatga cttgatgcag gacagctttt tcacaacctc tccttacatt ttaatggagc
     9721 cgatcacttt accagctgtc actgtaccta attgcttgat cactacttct tctactttat
     9781 cgtttgtaaa tacgatcatg atcgttggat ctttttcttc gttttggatc acatcaagat
     9841 ccatctcagc taatttcgtt cctgttccta cttcttggcc ttctttgaca gcaatctcaa
     9901 aagctggctg attcatttgg actgtatcga ttcccatatg gatcaaaact tcgataccat
     9961 ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat acttgtcact tttccagcga
    10021 tcggcgcaac aacttctctt gatgttggaa taacagcaaa gccgtcaccc atcatttttt
    10081 gtgagaatac cggatctttt acttcagata aaggaatcac ttctccagaa gctgcggaaa
    10141 ctagttcttg ttctatacct aagtattgct tactacctgc ttgtccattt cctgtagaaa
    10201 ccatttctgg ctctggaatc tctgctcctg attgcagcag atcttcgata tctgatttca
    10261 agacatctgc ttttggtcca taaactgctt ggactccatt gtctttgacg atcaatccca
    10321 tcgcccctgc tcgtttccag gcttcttcag tccctacttt ttctcgatca tttacactta
    10381 cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt ttcttttcct cccagcaaat
    10441 gaatgatctg gaccacttgc tgatctgctg ttcctgttcc gctgttagat acaacggttt
    10501 cttcactgtc attgtcgtaa tttccgttac gtcccggtgt agcaaagttg aattttttga
    10561 tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc gatcacacac aacacgaagt
    10621 tgataagatc tccacctaga cccgctttga tagctaacgg tgtccgagtc agcaattcga
    10681 tattcccaaa tgaatggacc cgcaatggca agatatctgc catcgcaaat gcagcccctt
    10741 ggatcactga ataaatgaca tataatggca cagcagcaaa cataaacatg aactccaatg
    10801 gttctgttac acctgttaga aagacagcca acgctgctga aaaatacatt gatttatatc
    10861 ttgctttctt atcaggatcg acattacgat acatcgcaaa agccattccc atcaagatac
    10921 ctgaagaacc aatcatttgt cctactttga aacgagccgg cgtccagtta tgcaacacaa
    10981 actcatattg cgacatatcc cctgcacctt taagattcac taaatcagtg gcccaagcca
    11041 accataacgg atcttgccca aatacttgtg tccccgcctg tgcgcctgat aaaatctcat
    11101 acgatccgcc aagctgtgta tagttgatag gaatcgtcag catatgatgc aatccaaatg
    11161 gcaatagtaa acgttctaat gttccaaata aaaacggtgc tagaatcggt gcgctttctt
    11221 gtgattctgc aatccataaa ccaaaattat tgattccagc ctgtacgttt ggccacacca
    11281 cagcaagaat caatgccacg attgttgacc ataagataac aacaaacgga acaaagcgct
    11341 ttccattgaa aaatgacaaa gcatccggca attttctata attatagtac ttattataag
    11401 ccattgcccc gacaaatcca gcgatgatcc ctacgaatac tcccatattc aaagctggtg
    11461 cttccaatac acttgtaaaa aatcctttaa ccatgatctt cgtaccgaac agagtgtggg
    11521 taaatgcatc ttcattcgca agcatgtcac ttgtgacacc aaagatagcc ccagtgatcc
    11581 gattgattaa aataaatgag atcccagctg caaatgctcc gcctgctctt tcttttgccc
    11641 aacttcctcc tattgctaaa gcaaataaaa tatgaagatt accaataatt gcccaaccaa
    11701 tattttcaat tactccgccc gttgtgacaa gcggcgtcca atcaggattg atcaagggaa
    11761 ttgatttccc tatactgatc atcaacccag ctgccggcat aactgctacc acaaccatta
    11821 aagcctttcc aaatttttgc caaaactcaa aactaaatat ctttttcatt ccgtttagct
    11881 cctttacgca atcggtttcg ttaaacgtat tatagcgtgc gttttctttt ttgtaaatgc
    11941 tttatttttt atttttctgt ctataccatt ctataaaacc tataatacgt gtgttccctt
    12001 ctataatttt ctcctaaaaa tttatttttt taagcttttt tctatgaaat attttctttt
    12061 gtctattgac ttgtgcaatc ggttgcgttt attatgaacg tataaaacag ctcattagga
    12121 ggagaaaaat gaaacagatc aaacgactat ttgctattga tccatggaaa atcagcacga
    12181 cacaattaga taaaaaaaat cttcgactgc aagaatcttt aacaagtatt ggaaacggtt
    12241 atatggggat gcgcgggaac tttgaagaaa cttactcagg ggaccaccat caaggtactt
    12301 atttggcggg tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa aatggttatc
    12361 cagaatactt tggaaaagtg atcaacgcct tgaactttat cgcgatagat atttcgatca
    12421 acgatcagct gatcgacttg gcttctttgg aaccagaaga cttttactgg gaattagata
    12481 tgaaaaatgg tgtattatct cgtacattca caatcactac ttctactaat aaagtacgca
    12541 tttccttcga acgttttctg agcattgtca aaaaagaatc agcttatatc tatttaacta
    12601 tagaaatgct tgaaggtagc ggaacagtca aactcgtatc aaaattggat ggaaatgttc
    12661 aaaacgaaga cagcaactat gatgaacatt tttggaatga gattgatcgt ggaactatag
    12721 aagaaatcgg ctatttgacg actaaaacta ttccaaacaa ctttgggatt gaacaattta
    12781 ctgttactgc agcaatgcgc catttcgtta atggaaaatc agtcatccct gactatcacg
    12841 aagatacttt agcattacat ggttgcttgt ctgcagaatt atctcaaggt gagacatggt
    12901 cagtggaaaa agaaattatc gttcttacta gtagagatat tccagaagcg cagcaagcag
    12961 aaaaagctgt ctcagaatta acgaaccaga caaacaatta tatccaagca aaagaagagc
    13021 aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa ggagacgaag
    13081 aagcacaaca aggtattcga ttcaatttgt tccaactatt ctctacttac tacggcgaag
    13141 atgaacgatt gaatatcggc cccaaaggat tcacaggaga aaaatacggc ggagccactt
    13201 actgggatac agaagcgtat gctgtccctc tttatcttgc tttagccaaa cctgaagtaa
    13261 ctaagaatct gctgaaatat cggcataatc aactgccgca agcaatccac aacgctcagc
    13321 aacaaggatt aaaaggcgcg ctttatccta tggtcacctt cactggtgtc gaatgccata
    13381 acgagtggga aatcacattc gaggagattc accgaaatgg cgcgattgcc tatgcaatct
    13441 acaattatac gaattacacc ggcgatacgg aatatttgaa aaatgaagga ctggaagttt
    13501 tagcagaaat cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga gcaaataaat
    13561 acatgatcca cggtgtgacc ggcccaaacg aatatgagaa caatatcaat aacaattggt
    13621 atacaaatac gatcgcagct tgggtcttgc gttacacgcg agaaaattat ttaacttatc
    13681 aaaaagaagc tgctatcact atcagcgaag aagaactagc caaatggaaa gatatcgcag
    13741 agaacatgta cttcccagtt gacgaagaat taggaatctt tgtccagcat gacacattct
    13801 tagataaaga tctcatgcct gtatccgata ttcccacaag cgatcttcct ctgaatcagc
    13861 attggtcttg ggacaaaatc cttagatctt gcttcatcaa gcaggcagat gtactacagg
    13921 ggatttattt cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac tttgatttct
    13981 atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg attttagcag
    14041 cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct cgcttggatt
    14101 tggataatta caataacgac acagaagacg gcttgcacat cacttccatg actggtagct
    14161 ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag ttgagattcg
    14221 caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat cgcggacgtt
    14281 tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa ggttctcctc
    14341 tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa ataacgctaa
    14401 gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt cattaccgat
    14461 actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg cattacgatc
    14521 gaccacacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact ctctttgctt
    14581 ttagcttatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga attagcgaaa
    14641 agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga tgtcttcccc
    14701 ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc tcttgcttct
    14761 gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc ttacacctta ctttgatgct
    14821 attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt cctccttgca
    14881 gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc gcaagccggt
    14941 atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc tgaagacctt
    15001 ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta cttgaaagaa
    15061 aagtggcatg accatggata atccagagat ctccgtcgta ccagttccag agactgaaat
    15121 aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt acggtgcccg
    15181 cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt tattgtctta
    15241 cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg tcggacctgt
    15301 agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa actgcggatc
    15361 acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag tagaagtttt
    15421 caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt ctacctatcc
    15481 aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag agatgacaac
    15541 ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct acttcaacct
    15601 ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt tagatggtgt
    15661 gcttgaatta gatcaagaaa aactgcctac tggaaattgg acaaaaaaag aaaaactgcc
    15721 aatcgacttt agaaaatccc caacactgca agagatcctt gcatgttatc cagatggact
    15781 agatgatgta tttcttttgc atcatcctag gctttccaaa acttccttac aattattcga
    15841 gaagcattct ggtcgccaaa tgactatagc aacttctaat aaaagtatgg tattattttc
    15901 tacgactgga ttcgaagctg atttctctgt caatggaaaa cagatgcatt caaactacgg
    15961 attagcaatc gaaccgcaag aaatccctga tatcgttcat ttccccaaat ttggatcgat
    16021 caacctccat cctggtcaag aacggatcag tcagacgatt tatcggttct ctgcacaata
    16081 acaagagaaa ggtgttttta tcatggctat taccgtaaaa gatgtggcga aaaaagccgg
    16141 tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat cctagtattt ccgagacaac
    16201 aaagaagaaa gtccgaaaaa ttatggatga actaggttat gttcctaaca tgactgcccg
    16261 caatctagga aaacgcattt ccagtgccat cggcgtaatc ttaccccctc ttgattcaaa
    16321 agagcggatc ggtaacccat tttatcttga gatcatggaa gcaatcaacg aagaagcgcg
    16381 gaattatgat atgactacag caatcgctac tgctaaaaat tttgaattgc tgttagagaa
    16441 cgtccagcgt atgcatttac aaaaacaagt agatggtttt attctagtgt attcagatag
    16501 tcaagatact gtcattgatt atttatacca aaaccacatc cctttcactt tgatcggaca
    16561 gccttatcat catgagaacg aaatcgttta tgtagataat gataaccagc ttctaggtaa
    16621 acaagcaaca gaatttttga tccagaatgg acataagaat atcctgtttg tgacaaacac
    16681 gactcatgaa agtttgtatt tcgaacgata tttcggttat caaaaagcaa tgatgatggc
    16741 tggtcttcct gcgttatcca gtgtagtact agagcatccg gaggattatc tgtcctttga
    16801 gaaaacgttg aaagaaaacg aagccacagc attggtggtc attgacgata tcttcgccct
    16861 tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt ccagagacac tctccgtcat
    16921 tagcttcaac aactcgatct tctcaacatt aacacaccct taccttacaa gtatcgatat
    16981 cgatatctcc gaattaggac gtgtcgctat gcaaaagctg cgcgaattga tccatgaaga
    17041 attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag agacagtcgt
    17101 tcctttagaa aacccatact aaatagctga ctcactaaat acgctgacca tttatcctat
    17161 ggtcagcttt ttatgtaaaa gaagatacta cttctaacac attgtttatc tgtaaaaaag
    17221 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
    17281 aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa aatcaatttt
    17341 cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttccttc
    17401 cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt aaaaacaggt
    17461 gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt catcgttccc
    17521 ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc acctgaatcc
    17581 gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga acgcttgaat
    17641 gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt gattccgata
    17701 cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc ttttaattct
    17761 tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc caatgcaagt
    17821 aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag cagttcatct
    17881 agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc tgcttttccg
    17941 accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga tcccgctagt
    18001 aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac tagtggaatc
    18061 aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc attgatacta
    18121 gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa cgaaaggacg
    18181 aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt aactcctcca
    18241 atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc taaaaaggca
    18301 ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg accgatcccc
    18361 aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt cactccgaca
    18421 attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag tttaaatcca
    18481 taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa gacaccaatc
    18541 aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat cccgtcatgg
    18601 atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat gaaagctgca
    18661 aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag gagtgccaac
    18721 aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat cgtcgatctc
    18781 ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg acctcctact
    18841 aaatcagcga caaaacgtcg gctgaaagac ttattttttt atttttcttc taaacttttt
    18901 tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg aataacgaac
    18961 atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt acctaatgta
    19021 gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca agctaatccg
    19081 tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt acgcatttta
    19141 agcaatgaaa cagcaatcaa aatgtacaca atacaataaa taacagttgt agcatttgta
    19201 agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc aaataatgaa
    19261 ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt ttctttatgg
    19321 aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt ttttgatggt
    19381 ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa gctgaagata
    19441 tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg ctgcgtgata
    19501 tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc attgatgaca
    19561 ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt ttttgttgcg
    19621 tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat aaatgtgata
    19681 ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt tgaccatgaa
    19741 aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa aacacctaaa
    19801 acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt cccgccgact
    19861 tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac taaaatacaa
    19921 cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt tcctaacaat
    19981 ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca taatagccat
    20041 gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg aggaccgcct
    20101 tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc ggcaaaaaac
    20161 aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa cgtaggaata
    20221 ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag acccatttta
    20281 cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa aataacggtg
    20341 tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag gggattcttt
    20401 gagtttattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga tcgaagagct
    20461 taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt attcaagctg
    20521 ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg tctgcgtaga
    20581 ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct tcaaagtgtt
    20641 cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact tttccggcac
    20701 ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg ctgttgccat
    20761 agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa atgcttcctt
    20821 tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca actaagtcat
    20881 cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa gttaatggat
    20941 gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag ttgtagaaac
    21001 gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca ttcaaaggta
    21061 agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa ccttccaaga
    21121 tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat gtcgcaaagt
    21181 aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca gaatatggaa
    21241 catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt gctttatatg
    21301 cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg tatttgtaat
    21361 gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag aaaatcgcac
    21421 ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg tctttttcta
    21481 atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct tctgttgatc
    21541 ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca cgaacgattt
    21601 tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga atcacttggt
    21661 ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta gtttgtggaa
    21721 ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga gctttgattt
    21781 cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt gacaagttca
    21841 ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc attgcaagcg
    21901 gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa gaaccatcag
    21961 caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc attcctactt
    22021 cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta ccattccata
    22081 gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc atatgacccc
    22141 agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct gaaatttctg
    22201 atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt ttttcttctg
    22261 gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa tgttcatcca
    22321 ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc ccaatgaata
    22381 gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc tcctaaaaaa
    22441 atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta aactaagttt
    22501 tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt cacacttttg
    22561 aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa gattgtatta
    22621 acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt aatagtattt
    22681 ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca aaaagtaatt
    22741 tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg gatttatgac
    22801 aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg aaacatcttc
    22861 acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca cgatattttc
    22921 aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga tttcttccgc
    22981 atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg cttctttcaa
    23041 gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt gtgcttcacg
    23101 tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt gagacaagaa
    23161 agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc agaattggta
    23221 gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg cagtttttcc
    23281 gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg cttctgcttc
    23341 tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc attggtctgc
    23401 tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt cgatagattg
    23461 caggatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc tggcaacgat
    23521 atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca aaaattcaag
    23581 taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg catctttacc
    23641 aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt gttccatcgt
    23701 ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg ttccgccgcc
    23761 aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca taaatggaat
    23821 caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa gtgaaatgct
    23881 cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa tggcgtcgcg
    23941 ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt tttatgaaag
    24001 caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg aaataaaaaa
    24061 agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat ttgtatgtct
    24121 agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac tgccaaagac
    24181 tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc agctttctga
    24241 aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt aatatgtgtc
    24301 tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt taaattcctc
    24361 attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt attgttaaaa
    24421 gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta ctttgcctct
    24481 tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa gcaagggacg
    24541 aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt aactatcgtt
    24601 attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga accaatcatt
    24661 cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca acaactagtt
    24721 actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc gttattggaa
    24781 aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact aaaaaaggag
    24841 aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt tttccacagc
    24901 ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata caaagattat
    24961 atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa tgattttgtt
    25021 ttagttaatt tgaatgattt gatccaaaaa gacgaacaag gaaatctttc ctttaacggg
    25081 cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt cagctattat
    25141 gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa caatgaaatc
    25201 aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat ggttcctctg
    25261 atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa aggtaccttg
    25321 gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca atatggagac
    25381 aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca gttgaagaaa
    25441 gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca agcttcacta
    25501 gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat cctaggaaaa
    25561 acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc ttattcagca
    25621 gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg taatgtcgat
    25681 gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc tcggatcaat
    25741 atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct tgatccattc
    25801 atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt ttacgtcatt
    25861 tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt tatgctttat
    25921 ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt atatggaagc
    25981 agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa atagaggatc
    26041 aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg atggaaaaga
    26101 tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga aaattctttt
    26161 ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt cactcgattg
    26221 agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg aagattttcc
    26281 gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa tacttcataa
    26341 atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac ctgagcgttc
    26401 tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat caagtcaaaa
    26461 gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc gttcatttgt
    26521 agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat tctgaggttt
    26581 attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg aattcattga
    26641 cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt ataaaaaggt
    26701 ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag aaaaaggagt
    26761 ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt tggtcgtttc
    26821 gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg atttatcgga
    26881 ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat aaaaaaagac
    26941 ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc tatccatgaa
    27001 cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg aggagcgatc
    27061 atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga tgaactgatt
    27121 caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg aaatcgccaa
    27181 aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt tgatgagcct
    27241 acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga aaaattgaaa
    27301 gaacagggca aatccatcct actttcttct catattctca gtgaagtcga gcgactagcg
    27361 gataaagtcg tgatcatacg cgaaggaaca ctcgatgaag aaggaacact cgatgaattg
    27421 cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag tcacttagca
    27481 caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt tgctgctgat
    27541 aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa aaaatttgaa
    27601 tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg aggaggaagc
    27661 gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct tagccgtgac
    27721 tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg ttttgtccct
    27781 gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac cttgcaaaac
    27841 ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga gtatacttta
    27901 ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc tgtgatctct
    27961 gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat tgaacttatt
    28021 cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga aagtattctg
    28081 atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca agcggattcg
    28141 atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg gattttgggg
    28201 agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc gaatggtctg
    28261 tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt agctgattcc
    28321 tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt tacaactaac
    28381 aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat cagttcattt
    28441 gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga aggaaaaccg
    28501 tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa taaagggatg
    28561 acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc tatctacggg
    28621 aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat ccaatctggc
    28681 gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg tctagctgct
    28741 attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg acgtatgaat
    28801 cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat gttactagct
    28861 attctatttg ctatattggc aatcctgatt agttctggtg cactagggct ttctgctttg
    28921 gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc cgggttcaat
    28981 ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg atggcttcct
    29041 cgaatcggta aacttgctta tatctactta gcttactcct ttgcactcaa ttactttggc
    29101 ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg ggttccacgg
    29161 atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat cagccttgca
    29221 ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg ataataaaaa
    29281 agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca tttggaggtc
    29341 atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact tctcgattct
    29401 ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa gtttcggggg
    29461 tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag tgatccttcc
    29521 ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact ttttccatca
    29581 tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt gctaaaagtt
    29641 tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt ctcaatatgc
    29701 aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg cctagcaact
    29761 ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc tttccgctag
    29821 cagataattg atacactttc atactgccgg aggccacgat cactaattga ggttctccat
    29881 tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt gcgtgtactt
    29941 tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca cataaatgat
    30001 catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa cataacttcc
    30061 cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa ccagatacca
    30121 gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg agaccttaag
    30181 taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa acaaaccttc
    30241 actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt gtctattgaa
    30301 atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt tcagcggtta
    30361 tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt tgaaaggttc
    30421 ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa tagtcagaca
    30481 aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa tccttgatat
    30541 cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct gcaatgctac
    30601 catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat tgtttttgat
    30661 tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg tacgcgctcc
    30721 aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt ttgctcatgc
    30781 cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat ttttgccagt
    30841 gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc tctttgagct
    30901 gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca agttccattg
    30961 tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg gacaaataga
    31021 ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa aactgcgatg
    31081 agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa gcgttgaaaa
    31141 taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct ctcctagata
    31201 ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct ggtcaatgat
    31261 ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa catctaacat
    31321 cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc cctgctcgat
    31381 cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg ctttcatcat
    31441 gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg atatcatcgc
    31501 catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt gaaaagcttc
    31561 ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc caatcggggt
    31621 atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt gcgtgagcca
    31681 ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg agatttttta
    31741 ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct ccgtcaagtc
    31801 gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac cgacttttcg
    31861 gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca tgaacacgaa
    31921 ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt cttttggtaa
    31981 attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt tagaaggctg
    32041 catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact cgatctcatc
    32101 ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc gaagcgctag
    32161 accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact cagctaggga
    32221 agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac ctgcaactcc
    32281 gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta cgatcacact
    32341 ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt tcaatgcatc
    32401 ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatcggctt ccttaatagc
    32461 cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg ctgcacttaa
    32521 catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc caccagaaac
    32581 caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt ctacacgatg
    32641 aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa tattgcttgg
    32701 atcattcatg atttttttca atttattccc atcctttctt tccggcattt tgtgcttctg
    32761 ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca aaagctcctt
    32821 ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc aacatgtctt
    32881 ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa taactagctg
    32941 catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg atctcgccat
    33001 ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc tcaatcattt
    33061 ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt tttttcacaa
    33121 gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt aatgaacaat
    33181 tcaagtatat aataaatttc taattttggg aaaatatgtg tagtgcttct tgaagtttgt
    33241 tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga acagtgtaga
    33301 gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta ctgggatcca
    33361 tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct caaaaagcta
    33421 tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg aacgtgcaaa
    33481 agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat taggcagcga
    33541 caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat aagtagggga
    33601 catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga attagatttt
    33661 tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc tttttgcgga
    33721 tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa ctgacagaag
    33781 ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc tctgtcgttt
    33841 gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc gaatgcaaaa
    33901 tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca aaatcattag
    33961 caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct gtgatatccg
    34021 cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat tgctgattga
    34081 attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc gcttttgctt
    34141 tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta tttttaagat
    34201 cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt gcagcttcta
    34261 atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg atttggtcag
    34321 aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct cctgaattat
    34381 ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca ccagaagagc
    34441 ttatggttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa gctgtctgtc
    34501 ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt ctttttttca
    34561 tcacttttcc catccttact actttttaac taatttctta ttataagcaa aaatgtttgg
    34621 aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt atttttttgt
    34681 acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga ctgctggaat
    34741 gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat atagtggtgc
    34801 gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag ctagcaactg
    34861 gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa aagtaatata
    34921 aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa aaagaactac
    34981 ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga agatgatcgt
    35041 tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca gccagacttc
    35101 tgttccacct tttgcccaac gttttctgtg tcgatatagc atcttcaacg tcacaggtac
    35161 ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta actgatgatc
    35221 ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca catcgatcaa
    35281 ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac cgaaaacagc
    35341 tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt gagattttgc
    35401 aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa tatgtcttgc
    35461 accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg gtaccgtgtc
    35521 tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat taaacgcgtg
    35581 ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt ttttcatcag
    35641 tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta atacctcata
    35701 gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tattcgatca caatttcttc
    35761 attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa catctaccca
    35821 ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa accatacaaa
    35881 tccgcctaaa atcggataag aaacgagaaa aaaatacata atagacaaac aagtatctaa
    35941 gaataaatta cctgtatggg tgattgagat catttcagtt ccaccccatt ttctttatac
    36001 aaatctgcaa tgaatgtcgt atctagattt ttctcttcgg agacagaata aaaacggacc
    36061 gtttctctct cttcttttgt tccaaaacgt tccgtataca cttcttcaag taattcttgt
    36121 ctttttttca atttttcatc ttcatattca aaagatttat ttactttttt gtatagattt
    36181 cgattgttcc acctagtcaa tacaaatgag gtaatcagga aactgaaaaa aatcaataca
    36241 aaaaagccga ttaagaaata cagcattttc ttttctgata aaaaagcgta caaggaaaaa
    36301 tccttggctc tttctggtct cgttaatggg aaaaagaccc atataaaagg aagaacaacg
    36361 atcatccatc ctaaaagagc cactaacgtt tgccaaatct ttaaccagta attacctttt
    36421 gaaaaatact tatctttcac caaaattgat gcaacttctt ctcttgttac ttttttcata
    36481 tcctcacctc acgttttttt gttacgacgt aggagtttac catacatttc atttcttttc
    36541 acataaagtg ttcctaaatt tttcctaaga aaaagaacaa aacatttctc atcagcaaat
    36601 gaaaaatatt ttgttctttt tatcagttgt tctttttatt ttttatctct taattatgtt
    36661 gctctttcca atacgacaat gcttttcatt cctctagata tcgccgtgta catttgtctg
    36721 cgtcttcttt gctcatctcc atcgttttca aatggatata agattacatg atcaaattcc
    36781 agtccttttg ctagactcaa aggaatgatt tggcagtttt ctaatccata tgaatttttt
    36841 ttaatcgatt cttgtagcgt ttgggtttcc tcttctgttt gggtgataac tgccgctttt
    36901 tctcctttta gagaaaccaa aatctcagct agtattttca acaattcttc agaggatcga
    36961 aaagagcggt attcgggttc ttttccttgc ggtcggatcg atacgatgtc gatcttctct
    37021 tgatccacag cataggtttt gaaaagctct gtgattgctc cgctagaacg gtaactattt
    37081 ctaagatcat accgatggat cggcagatgg tagtcgtcga aacaacgcat aatatcagca
    37141 aaagtagtgg aagaattaaa gattgcctga ttctcgtcac ctactaaagt aaagtgagtt
    37201 tttggaaata attcgatcaa tagacctaat tgtgccaggg tataatcttg tacttcatca
    37261 atcaatagat agcgaaatgc tggtacattt accttttcaa caagtaaatg acgaatagct
    37321 aatataatga ctgcttcatc aacagtacgt gtgggttgcc aagcataagc tgagccatat
    37381 cgtttttcat aaattctttc aaacaactga tgctcatcca cccatgccat ttcttcgatt
    37441 tgtctggtta tttttcgata ttttttttgt aaaagtttcc ttgcataagc tacaatactt
    37501 tgttccgaat catcagaaat caatttaccg aataattcca actgcctatc ttctgtcaac
    37561 gagagtacct gattctgcac agcaggactt tttgcttgtt tcaaaagatg attcttccat
    37621 tcgctcatca gtgccttttt cgttgcttga agcctatcga tcaatttagg atgagcagga
    37681 actttctcat atattttttc gatcgttttt ttagaaagga ctgtttttcc atttttagta
    37741 agtccacgga taaaattcgg atgatctaaa aacaaaggat ctgattgttt tagattatcg
    37801 ataaactttt tgctacgcaa acgttctgtc tgctccgata cattttctcc cgtgatacgt
    37861 ttgaaataag cctcttcacc ttctatttcc tctgctgaaa gctgtgaaac cagctgtatg
    37921 atggttagat tcagtggatt tctttcacct aatgatggta ggacattcga aatatagtcg
    37981 ataaaccgat gattaggtga tagaatcagc aagtcatcgg atgtcatcgt atctcgatat
    38041 aaatagagca gatacgcaat acgctgcatg atagcagagg ttttaccact tcctgctacg
    38101 ccatttacca agatattttg acttttcaca tcccgaatga ttgtgttttg ttccgattgg
    38161 atcgtagcag tgatagcctt catttcattg gtctcatctt ggcctaagac ttctaataat
    38221 acgtcatctt gaatagctac agacgtatca aaaaacttga ccaaacgatc tctttccaaa
    38281 agaaattgtc gtctttcttt aataaccgta tcgattttat tttgacgaac cgtataagaa
    38341 gatggaccaa tcatgttatt gtaaaaaagt tcggcaattg gtgaacgcca atcataaaca
    38401 agattttcat ctgcatcatc tgtgaaattt gctgtaccga tataaaagga ttcttttggc
    38461 tcattatcca gaaagtctac aacgactttt ccaaagtagg gacttttcaa taaacgttcg
    38521 atctttgcta gctgattttc tgcgtttttc tgtttgatat tcatctgatc gatttctcta
    38581 tttttcattt caatcatcga aaaagtatcc aggttatcta agtaactgtc aaaattcaat
    38641 cgaagctcgc cagccatttc tttcaacgac tggtttcctt ctatttttgc cgtatccatc
    38701 gattcccgca gttcgttttt tttatcaatt agcttttgat acacgttctt cagatatgct
    38761 tcttctaact gtttttgtgt gttgaccata cacattctcc tcttttttta aagtggtgga
    38821 accgattagt ttacctcttt taagagtcga atgcaagctt tttaacgagt atcatttgtt
    38881 aggatatatc agttcttcag gtcggacagt ttgatataaa gaaaatgcac catcaaggtt
    38941 ttttacgtgg tagccagact gcttcaagat tctttccgct agatagctac gtaatccgct
    39001 atgacagctg acgatatatt cttgttggct gtctaattca tttaatcggt cccgaagttc
    39061 attcaaaggg atatgtttgg cttccttgaa tttgcccttt tccaattctt ctgctgttcg
    39121 cacatctaat aaaatttttc cttttgctag ttctgtcggc aattcatgcc attgtacggt
    39181 ttcactaagc ccttctgcaa tgttcattgc tgcatatcct agcatgttta ctggatcttt
    39241 ggctgagcca aatggcggtg catacgtcaa ttctaattcc ggtaaatcga agatcgtcaa
    39301 atgccctttg attgctgttg ctaaaatatc gatccgttta tccacacctt ttgccccaac
    39361 accttgtgca ccataaatct cgccagtctc tggatggaag atcaacttca gtaaaatatc
    39421 tgttgcttct ggataatagc ttgcatgatc ttttcctgat acatgaatga cttcaaaaga
    39481 cagttcgttt tctttggctg ttctttcact taaaccggtg gatgcggcag tcattccaaa
    39541 cgttcgtata attgctgttc caataccgcc ttgatttctt cgagaaattc cggaaatcgt
    39601 gtccgcaact tgtctacctt gacgattggc tggtgaagca agagaaataa gtgcatcttt
    39661 tccagtgatt tgctgtttta caacgatagc atcccccacc gcataaatat caggaatatt
    39721 cgtttgataa cgttcgtcta ctaggatacc cccgcgtatg cctaattcga ttccagcggt
    39781 cactgctagc tggttatcag gacgaacacc aatggataaa ataactaaat cagcagggat
    39841 ggtacgccca tcatccagtc gcaatcgttt cccgtggtct tcaaatccag cgacagcatg
    39901 agaagtaatc acttctactc cttgatgaga caattctttt tctataaaag ctgccatttc
    39961 ttcatccagt gttggaagaa tatgtggtgc ttgttcgatc accatgactt ctaaacctct
    40021 tctttttagg ttttctgcca tttccaatcc gatgaatcct gcaccaacga tcacggcacg
    40081 ctttggctgt ttttctaatg catgcatcac tttatcgata tctggtacat ttctaataga
    40141 aaaaacattg tcggcttctg ttatccctgg aatcgatgga acaactggtt ttgcaccagg
    40201 agataaaacc aatgcatcgt actgttcaat gtgtttttga ccctcatgta cgacttctac
    40261 cgttttgttt tcgggaaaga tggctgtcac ttcatgattt ggacgcacat ctaaacaaaa
    40321 acgagcggat aatgattctg gcgtttggac aagaagattt tcccgttcac tgatttctcc
    40381 cgaaagataa taaggcaagc cgcaatttgc aaaagaaaca tacggtcctt tttcaaaaac
    40441 aacgatttct gcatcttcca ttaatcggcg aagccgtgta gcagcagaca ttccacctgc
    40501 tacacctccg acaataacga ttttcatctt atttccctcc tactgttcga tcaaaccatt
    40561 gattcatgcc accacgtaca ttcactacat cataaccaga tttctttaat tctttagctg
    40621 cttgtttact cctcatccct gattgacaaa tcacatagac ggtcttttct ttgtctccat
    40681 catatctgtt tatactttgt aatggaacat ttttagctcc ttttatatgc ccgccccggt
    40741 attctgcagg tgttctaaca tcaagtaacg ctggattttc caaaagcttt tcctttaatt
    40801 ctgggactgt tatcgatttt attgattgaa atagcaaatt cattttctgc acctcattta
    40861 catttttata aatacctcat ggggtatatt aaacaggagc tttattaata tgtcaaccag
    40921 aatgactctt catttttttc actacttttt atatttgcat aaaataccga taaggggtat
    40981 tatctaatta taaaaataga ggagaatgtg ttatggagat tgataataaa aatatcctga
    41041 accgtttgaa acgtacagag ggacaaattc gtggtatcca aaaaatgatc gaagacgaaa
    41101 aagaatgtat ggatgtcatc actcaattaa gcgctgttcg ttctagtatt gatcgcgtaa
    41161 tgggcatgat cgttgcagat aatctgaaaa attgctttga gaaacctgat agtaatccag
    41221 aagaacaagc ggctaaacta gaacaagcga tcaagatgat tattaaaaag taaaggaatg
    41281 atttcaatga aagtcgtttt ccatattgat gaattagaaa aatggtcaga aacagggaaa
    41341 aacgtaaaaa acctgataaa agcttctcca gaaacgacga ttgtcgtatc tgtaaatggc
    41401 atcgcgatta ccggttatct agactctgcc aatgcagaat tcttagattt gcaaggagtc
    41461 gtttttcacg cttgcgccaa tgctatgcgt gctaatcata tttcagaaag ttctttacct
    41521 gaacaagtaa tcgttgtacc agcaggtgtc cttgatttag ttgaactgca atcccaaggt
    41581 tatgcttata tcaagcctta gtcagtagag gttgcgacag aagtgatcag cttcaagaaa
    41641 tttcggctta tttttcgaag aagctacttc tggaaaaccg tgaatcaaag gttgtgaaaa
    41701 cgctgtcctg catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcgt
    41761 ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg
    41821 aaatcagcgg tttgacctaa gtattagaga aaaaaacggg aaaatagctc ctcatatttt
    41881 gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttaag gaagagattg
    41941 tgaaaaagtc gcttagtttc gagtattaag aaaaattttg tgaaaatgga tttctacatt
    42001 tttgcataaa tttggcttaa taccgagaaa ctggcttttg aataccgttt aaggaagagg
    42061 ttgtaaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agctgctcat
    42121 attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt ttaccaagag
    42181 gttgtgagcc taccatttaa ctccgaatca caagaaataa tttcaaaaac aacttctcct
    42241 gtttttggtt cggttggctt attatcgaga agtagctact gggagtctat ttatcaaaaa
    42301 aaggaatgcg acataggtca cacccctttt ttattttctt tgaaacatct ttcggtactc
    42361 gacagcatac aagacaaaag attgtaaaag accgtaagtg atgacggcaa aaatgactct
    42421 cacccacagc gttggtaatt ctgggaaaag cagcaaaagg atcatgccaa catagatgac
    42481 agcagtcgct actttgccat accacttcgc tccacttaat ttgatatttt tagatagccc
    42541 gattaatcca gctacaaaca taaagccttc ttttatcaag aatagaccaa acaacagcca
    42601 aagccacggt cgataccatg ccatagacaa aatcagaaaa agttgtgtca atttatctgc
    42661 aaacggatca agcaccttcc ctaagtctgt tacctgatta tatctccgag caatgacccc
    42721 gtctagataa tcagacaaac ctgagacaac aatgaccgct cctgctatat aaaatgactg
    42781 tctcagcaca aaataaagat aaatcggcag caaaaaaata cggaatatcg ataaaagatt
    42841 cggaatcgtt tgtatttctt tacgccagtc tgtgcttaat ttcattttat cgccctacct
    42901 cctcagcact cttttctttt tattttagct tacacgggga ataaaaccaa tgatttcact
    42961 gtttttattt ttctttacag acactgatat tatgagctaa tttgagataa tcacttgctg
    43021 ttttcatcgt ttactcttct ttcttcctgt cgtttgcgca tttcttttac tagtacttta
    43081 tgagcttttt ttgatcctcg ctctaggtca cgctgccgtt tacgtgtttt ataatcgata
    43141 aacagactgt ctaaatcgta acctgtcggg tacagatctt ctgctgaacg caccagacga
    43201 acacgctgac gaggcacgtg gattattttt ttgtctaaat atacatcaat cgtatcttca
    43261 cctatatctt cataaactaa cgcttctttt tggtattcat tgacaaatat ccgatcacct
    43321 tttgcaaaga gctgacgttc ttttttattc tccttcctat ttgtaactgc tgtcttagga
    43381 agaaattctt ttttctgtgt ccgataggct ttttccgcca agtaacgctc tgcttgatgg
    43441 atcaatttca tcgacatatc cattttatga gcaatccaca aagcttggct ttctcctgtt
    43501 tcttccaacc cgcaactgat atttaggacg aagtgcttcc cgatcgaaag ccatagcagc
    43561 cggcacaaaa tcttcatgat ctccggcgaa tttcttgatt tctccataat gggtcgtggc
    43621 aatgatcaat gccccttgct catacataga ttccatgatt gcgatcgcta gtgcagcccc
    43681 ttcatttggc tctgttccac tgccaatctc atctaaaagt accaacgtat tcctttttat
    43741 ctttttcaaa atagcagcga tgttttgcat atgaccagaa aatgtactca acgcattttc
    43801 taaattttgg tggtcaccga catcgacgaa aatctcatca aagacagcaa gctctgtccc
    43861 ttcttttgcc ggaacttgca taccgaacat cgccatcaaa gtcaataaac cgactgtctt
    43921 caacacgact gttttgcctc cggcatttgc tccggtgata atcaagcctc gatagtcttt
    43981 accgatttca acatccaatg gtaccgcatg ctcaggtaaa aatggatgtc tgccttgctt
    44041 gataatgata tgttccgatt tattgattct aggtgtgctt ccattgattt ctcggctaaa
    44101 ttttgcccga gcaaagatga tatctaacac cgtgatcgtt tcgatcaata aatcgatcac
    44161 ttcttcgttt tctgcgatag caccagtcaa tgccgccagg acttgatatt cttcactgat
    44221 ttcttcagct tttaacaact gatagtgttc attcaattta gaaatcacag ttggttcaat
    44281 aaaaaccgtc gttcttttat ttgattcatc gataatcgtt ccatctattt tatttttata
    44341 gctggctttg ataggaatcg tatagtattc gcctttttga acaatcattg cttcttggat
    44401 catttctttg tttttcggat gacgcaagaa tttcagtaat tttgactgga tttctttttc
    44461 gattagttgt aattgtttgc gtactttacg caaattacgt gacgcgtcgt cactgacttt
    44521 attatttttt attttctggt caatcaactc ttctacattt atcaaatcag gaagatgttt
    44581 gctataagca aacaacaagg gtgtctggta ttgattctta tcaaaaaact ttgtgatcat
    44641 ccgactgctt ctaagaaaat ccgcatattc gatcaaatcg gttggctgca gaatcaaccc
    44701 ttttttgact tgttctgtca gtgcatcgat cctcggcaac cccataaatg gaacatgctg
    44761 actgctttct aagatcaagc gtgcttctct tgtttcttct tgacgatcca agacagtttg
    44821 taggtttgtc gagaccgtca tatcgctgat tcgttttttg ctatagtttc caattgctct
    44881 tgcttggatt tcctttttga tccgatcaaa ttgtaattga ctaatcattt cgttgtccat
    44941 attctctcct tttcttgaag agatacctgt cccaaaataa ataagggcct agagataaat
    45001 aaaaggatag aacacagctt aaaaaagtca tgtcctatcc ttattagaat acaaagatat
    45061 tgttttcctg acaggtaccg tttcataagc acaccaaata aactttgttt attggctatt
    45121 cgacttactg attagatacc agctacactc acaaaacata ccccattctt ctaggtctta
    45181 tttattttcc acttaactat agcaagacac attttggttg tcaaacttta gtatgtttta
    45241 tagatttaca ccattaatga atagacacct accgcgataa ttgctccgat cattggacct
    45301 gcgattggta cccatgaata tgcccaatct gaagttcctt tattagctat tggcaatacc
    45361 gcatgtgcta atcgaggacc aaagtcccga gcagggttga ttgcatatcc tgttggccct
    45421 cctaatgaaa gaccgatggc taagatcaat ccacctacag cgaatacgtt cgttccaggt
    45481 gcaaattcgt tttgtccaaa agctagcaac ccaagaacta atacaaatgt accaattaat
    45541 tctgtgatca catttgctgg ataattccgt atagcaggac ctgttgcaaa cgttcctaaa
    45601 atcgcgcttt catctttcgt tgcattccaa tgtggcaaat aagaaagcca aacaagaatg
    45661 gcccctgcaa aagctcctaa aacttgcgca atgatgaaag gaacgacaag attccaacta
    45721 attgcccctg tcatcgccat agctagagag actgcaggat tcaaatgagc tgggcccatg
    45781 aatccagaaa tatagacagc cattgttaca gcgagtcccc aaccaaatgc gatgacaatc
    45841 cagccagaag cctgtgcttt acttttgttt aaattcacag ccgcacacac accatctcca
    45901 agcaaaacta ggatcagtgt cccaataaac tcccccatga tttgagtcat atcactgttc
    45961 atttatttct ccccctttag ttcccgcaga tctgattctg caatcaatgc ttctaaacgt
    46021 ttagtctgtt gcgccttctc ctcttctgtc cattcaaaat atccagcaat tgcatcgatc
    46081 acaggttgct tgatctcatc aagctgatct cgttcaaaca acagatgatt tgtacgacga
    46141 atgagataat cgcctggggc caaaaccatt tcttcttcta aaccataacg taaccgagct
    46201 gactcagcga gactcaaacc aggataaggt gccatttctt ttgctaattc aaagatacgt
    46261 cgggcattag tcccgtaaaa atcagcgata taagtagcat cttcttcctc taaaccggct
    46321 gctactcctt cttttgctaa ttctgtcact gtttcttcta atttagttgg atcgaagttt
    46381 ccacctgaaa tctgatattt tttagagtcg atttctttcg tctctattcc gtattcttct
    46441 tttaataatt ggcgtatcaa tcgcaaagct ccttctgcca tcttacggta atcggtgatt
    46501 ttcccaccag ataaagtcag caaaccatct ggttctcttt ctaaagaact acctctggaa
    46561 atcgtagaag gtgctttttc atcacggctg ttttccaaat ggttcaacac gtcttctact
    46621 tcagcacgag aaactttgtt ttccttatat tcacttactg tatcgacaac tttattgaag
    46681 ctcttgtctg aaatcgatcc attatctcca ccattgtaat cagaaccaga attaccaatc
    46741 aacagtggac gaagtcctgc ccagcttgct tcgatatcat tcaatgtgat atttgcttct
    46801 ggatagcgat gattgatcac atccaataga taatctacat cttcttgtgt gactttcgga
    46861 tccgtaaaat ctccttggta atccgtatct gtcgtaccaa agtaagtctt gttttctctt
    46921 ggaatagcaa aaaccatccg cccatcttgc tttcctgtat cgaagtatgt gggttgcggt
    46981 acaggcagtt ttttcgcatc tacaactaaa tggatccctt tagttggacg cattttagga
    47041 gagactggac gcgtaaaatt caagtttcgt actttatcta cccaaggacc actcgtattg
    47101 atcactaatt ttgctttgat ttcgatcact tcatctgtca gcaggtcacg agctttgacg
    47161 ccaacgattt gatcgccatc atataaaaag ccaaccgctt tcattttact tactagataa
    47221 gccccatctt cggcagcctt tttgatatta tcaatcacta aacgggcatc attgttgcgg
    47281 aaatctagat agacacccgc gccttttagc ccttcttttt tcaaaaacgg ttctctttcc
    47341 aatacttctt ctggtgtcag cgtataattc tcatatttgg ttcctgtcac atttgccagt
    47401 ttgtcataaa ggtccattgc tactttgacc gagaacatat tgaaggttgt tgctccttcg
    47461 ccctcataga ttggcaaaag cattggatct ggttttggga tgtgtggggc aatactttgt
    47521 acgactgcac gttcaccaac tgtgtcagct actacttcca catcaaatgt cttcagataa
    47581 cgaataccgc catgaacaag tttggtcgag cgagaggaag tcccttctgc aaaatcctgc
    47641 atttcgatca atcctgtttt gattcctgat gctgctgact ggactgccac accggcacca
    47701 gtgattccgc caccgatgat caacagatcc aattcttctt gctgcatttt ttgaatgcta
    47761 tcttgtcttg tcttgtttga aaacatatgg atttcctcct tattcaaatg cttgggtagc
    47821 ttttactgct tttttccaac cgttgtaaag tttttctcgg cgctcttcct ccatttgaac
    47881 ttcaaatagc ttgccagctt cataaaattc gcggatctcg tctgtatctt tccaataacc
    47941 gacagctaat cctgctaaaa atgctgcccc tagcgccgtt gtttccaagt tttctgctcg
    48001 ttggatcggt acattcaaga tatctgtttg gaattgcatc agatattcat tgtttgctgc
    48061 accgccatct actttcaata caggaatctt gattccagtg tcatcttgca tcgtatcgat
    48121 gatatctctc acttgataag caatcgactg taaagtagct ttaatgatat cttcttttgt
    48181 cgtaccacgt gtcaagccaa acatagcccc tcgtgctgcc tgatcccaat aaggagcacc
    48241 tagcccaacg aatgcaggaa caacataaac ctcatcttcg cttgtagagc gtttcgctgc
    48301 gtcttctgaa tcgctagctt tttgcaacat ttgaagtcca tctctcagcc attgaatcga
    48361 agaaccagcc acaaaaatac ttccttctaa agcatagtaa acctttccat ttatgccgta
    48421 accaattgtc gtcaataaat tatttttgga taattgcggt ttttcaccgg tattcatgac
    48481 aataaatgaa cctgtaccat aagtattctt gaccatgcct ggttcgaatg ccatttgtcc
    48541 gaataatgcg gcttgctggt ctcctgccat ccctgaaatt ggtgcttcgc ttccataaaa
    48601 atgataacct tgcgtataac cataaacttc tgagttagaa gtgacttttg gtaacatcac
    48661 tcgaggaata tttagtaaat ctaaaatctc ctgatcccaa tctagctgat ggatattgaa
    48721 caacatcgtc cgactggcat tcgagtaatc agtaacatgt gcttgtccat cggtcagttt
    48781 ccatactaac caagaatcga tcgttccaaa catcaattcg cctttttcag ctcgttcttg
    48841 cgcgccttct acatgatcaa gaatccagcg tatcttcgtt gctgagaaat aggcatcgat
    48901 cactaaccct gttttttcgt ggaaaaattc ttgatagcct tcatttttta agttatctgc
    48961 aattccagct gactgtctgg attgccaaac aactgcatga tagattggac gtcctgtatc
    49021 tttttcccat attactgttg tttcacgctg gttcgttatg ccaatcccag caatctgatt
    49081 tggtttgatc cctgattcga tgaacgctcc ggcaataact gactgtacag aattccagat
    49141 ttcatttgca tcatgttcca cccaactttc ttgcgggaaa tattgcgtga actctttttg
    49201 agaactgccg atgtgtcttc ctttcttgtc aaagataatt gctcgtgagc tagttgtacc
    49261 ttggtcaatc gccatgatat acgttgattc tgtcattgtg attccctcca ttatatgcaa
    49321 acgctttctt taactactct tatcatacta ctgttttaaa aaaagaaact gtcaaaattt
    49381 tattcttttt ttcttttttt gacagttttt tattatgaag ctatttttta atcaattctt
    49441 ttcttttttc agcgtctcta ttcttctttt caatcgaata atgtcgtaag gtgacgcaaa
    49501 ttctgacaga taatattcac actgtttttt gtttctctgg caaaatgtga tcaccaaatc
    49561 atattcttta ccttcttcat aacttccaat ctcaataccg ccgattcccc tcagttctct
    49621 gatcagatat tgttgaaaag caatacgata aatcggcaga ctttctaaat cgattccaat
    49681 cactactggt tttgcaatat aaaaatcgat catcaaaaga acattggcat actggatcat
    49741 caggtaacta agctcactat cttcagctct tttcctcggc aattgctgca ccgctgtttc
    49801 ctgtaatttc gccagtaact gtgttaagga atgcccgagc attttctgct gctgttccaa
    49861 aagccgatca cgctcataga cttcgatctt tccaacaaag aaaaaccatt cattgttgat
    49921 ctgggaaatt tggtagccta ctgccttttc ttctttgatt ttcaaacgac gaggacgata
    49981 atgccataac atcgtttccc gaatgtaagt atctaaaaca gcggttggta actttttgct
    50041 ccgtgtcaaa tcatactgat aaaatatttc ttcgtctact atcgaaaaag aaacaaaaaa
    50101 gctgtaaaac atcattgttt cgtatacatt cagttctgct gctgttcgac taagatgcat
    50161 cacgatgatt ggatcgattg cttgataaag ccggtccgac tgatagagta atttcttttc
    50221 tttcaaagtt gcgtacgtac ttttttcatt caatagtctt tttttcgtaa tcccgagcca
    50281 gcaagcaatc tgtgcttctg ctgatgccgt aaacgttgtt tctaatacac ggtccaatcc
    50341 tagaatgaac ggtctttttt ctggtgtatt ttgcaaaaaa agcggtcgct gatcttccgg
    50401 gatatattgg aatagttcat aataaaaata tcgtatctgt agctcttctc cgtataattg
    50461 accgtttttg atttgtaatc caaattcctc taagagctgg ttcaattctt tgatctttcg
    50521 aaaaacagaa gactcactga tattaaacgc tgtcgctaat cgaacgatgg atacttgctg
    50581 atgttctaat aaatagacga gcatcttaaa cttcaagctt tcctgcaata aaaacaatag
    50641 aattttttcc aaacttacat cttcgtccaa cacaagcagc gcctgatact catttcgctg
    50701 tacttctact tttttttcca tcattcggcc aatcaattca atatcttcca aatattgatc
    50761 aaacgaggct tttgacagtt ggacatgctc tctcaaacca ttgatagagg ctgttccccc
    50821 ttgcagaaca aggtttcgca aaatcaggat ctgtaactgc tcttttcttt ctaataatgc
    50881 ttcaatcttc ataggcagta ttcgccacct ttgaccacac catatctggc acacggtatt
    50941 ctggctggaa aaatagctca tgttcttcta actgttcaaa cgtccatcct tcttctactg
    51001 caattgtcag ttggtcaaga aggtggaaca tctctttttt gctttggagc tgtgctccaa
    51061 gaatcttttt ttcagaatca aatagaaatt tgagtttaac tttgctttga tttattgctg
    51121 taagcttgta gtctacgtat cctgtttcaa ttttttgggg ataaaaaatg ctctcttctt
    51181 ctgtcaaacc tacgcttcct aagaaatagc caaaccactg attgccgact ggacgatagg
    51241 ttccctgatc ctttgtctgt gagccactaa ttgtcttgct gacatttcgt gctgtacgga
    51301 cagcgttatt tactagagaa acatacattt tttcatttgt tggacgaaaa gttacttgga
    51361 tcgcatcgcc aatcgcataa acgtcttttt gagacgtttg caagtagtca tctacccaga
    51421 tcgttccatc atcattcaat gttaattgtt cttcccacat ctgattgtcg ggacgagaat
    51481 gattagccag caaaacaaga tcatagttct cctttctttt ttccgtctct acaatagcgg
    51541 tttcattttc ggtcaaagac acaacttgtt cttctaaaaa aagtgaaagc ccttgatttt
    51601 tcatttcctt tactaacggt tctgtcattt caggatctag ataacgaaaa agaattgact
    51661 ttcggctttc gtaaagatgg atttgttttt ttcttgatgc caacccttca gctatttcga
    51721 ttcctgcttg tcctgcacca ataattgcaa ttttctttgc attcatgatc ttttgctgaa
    51781 gagccgttac gttttgtatc tttttaactg ggtgaataag cgttgattct gcagaaatat
    51841 ttcgaaactt ttgatttgaa ccagtcgcta gaatcaaacg atcaaaatcc aatttttttc
    51901 ctcctgctaa aacgactttt ttttctttca atcctattac tgtcttttct gtgtaaacat
    51961 ggattcgata gcggtcagtc agttcttctt tcgtgatcca agaaagttgt ttttcactgg
    52021 taacctttcc ttttaatatc aaattgaacc cgctgggaat ccatcctaac ttcgattgct
    52081 tttcaatcaa atatatttca cttgttggca tcaattggcg aagataaata gccgcatgaa
    52141 tccctgcaaa cgacccgccg acaattacaa tgttcatctg cattcctctt tcttataagg
    52201 tgttctactg ctaacttctc aatcagaaaa cacagtattc ataactcgct ttcaacctta
    52261 tgatacatag cgatgcagca gaatcaaccc tctccatcat accgtgttct aggtctgctt
    52321 ctcaataatg tactagtgga cctaattgaa gagtatcatt tctgaagaga tgtttcaaaa
    52381 aatgtactct tataaaagaa aaaaactcgc cttttacgta cctctgtcca aataggaaac
    52441 agaaaagaac gaaaagacga gttttagcgt gtgtaatgta atagttagaa tagacacatc
    52501 atttattttt aattctttaa tgcttcaaca actgcttcgc ctaattgatg agcagattga
    52561 ggattttgcc ctgtaagaag ccgcccatca atttgaacca cttctgaaaa agctgctcca
    52621 gactgatagt ttgctccttt ttctttcaaa gcatcttctg ctaagaatgg tacttcattc
    52681 gttgtaccat ttgcttcctc ttcttcatta ctgaacccag tcactgtacg tccagctaaa
    52741 atagattttc cagactcatc tgtaactggc aacaatccta caacaccgtg acaaacagct
    52801 gagaccagcc cttcatttcg atagatttgt gaagcgactt tcccaagatc tattgcttct
    52861 ggaaaatccc acattgttcc gtggccacca gcataataaa ccgctgcata atcatttgca
    52921 tccacatcat caggacgtaa ggtatttccc aaggcccgat ttctgaaatc ttcatcttca
    52981 taataggccc aatcgatttc atccatattc aaaacacttt gcggttctaa aggtacatat
    53041 ccgcctttag gactgacaaa atctgcatcg atcccatttt ttactaatac atcataaaaa
    53101 tgagtcagtt cacttaacca caaacctgta ggacgctcga tcttttcgta cttttctaca
    53161 tttgttaata cgatcaatac tttcattttc ccatcctctt tctataaaaa ttgcgttatt
    53221 gataacgtgc tttcaagaaa ttatataatg ggcttggcaa ataaagctct ttatatgctt
    53281 cttcaaaagg tttcgttaca aattcatcct cacggttttc caataatttc tctgcccagt
    53341 caggatcgac gatcatttgc tgtccaattg ctgcaagttc tgcatatttc aatacctgct
    53401 ctacatcttc gcgattgcgg atcccaccga caccaactaa agggatcttt ccttgaatcg
    53461 tgttatggac cacttcaaga atcgatttat cgttgtattt atcagaacga gctattcgat
    53521 catacatatt taatgaaaca tgcagataat ctagttttga ctcacgtaat cgttcaagaa
    53581 gccagatcgt gtcttcaaaa cggatacctg gtgtttcata ttcttctggt gagaaacgat
    53641 accctacgat gaatggtttt ttagcatatt gttcaaccgc tgagaaaacc gttgttaata
    53701 attcttcaat aaaacgataa cgattttcca agctaccgcc ccattgatcc tctcgtctat
    53761 tcgaatgcgg agagaaaaat tgttggatca aatacgtatt tgctccatgc aactcgattc
    53821 catcaaatcc tgattcaatc gctctacgtg tggctttgcc aaatgcttgg attgtttcaa
    53881 cgatttcttc ttcactcatt gctcgtggtg tttccgcatc aggacgtttt gctgcaactg
    53941 cacttgcaga tacaggctgt tctccagata aagttgctcg acttgtcatt cgtccaccgt
    54001 ggaaaatctg aataatcgct tttgcgcctt ctccttggat tgctttcgct agctcactta
    54061 atcgaggaat catttcatca tgagcgatgc tgagttcacc tggccagcct tttccgatat
    54121 cttggacatt tgcagcacca gtaataacag caccaagacc tttagaacgt tgtttataat
    54181 aagtgatctc atcttcggtg atcactccat tgaaaaagct gagttgtgtc gtcataggcg
    54241 acagcatcaa tcggttcttc aattgaactc ctgaaggtaa agtaattgat tgatcaaatt
    54301 gtgacattgt cattcctccc ataattcgct ctgattaacc tatcgagaat catctatcat
    54361 taaaccactc gattagttgt acatgcaact aatttgctta aaaaatttta ttttttttgt
    54421 aacttatctg ttgcatcatt cattgtctgg gctaattctt tacttgcttc atttcccaaa
    54481 actgctttgt atgactgatg aagttcttcc cagctaccat cgacttcctt agcaaattgg
    54541 cgtccttgtt ctgtaagata aactaatgac aagcgacctt cgctaactgt tttgacgtat
    54601 cctttatatt ctaatttttc aacaaaacga gcaatcgttg atggggcgga atagagcatt
    54661 tgtgcaatat ctttttgtgg tattccatct ttttctttta atatcaataa aataaaacca
    54721 taagttggtg ccagacctgt ccttctaaac gcttcatcag ctaatttatt caacatgcga
    54781 tctagctttt tcacgctaaa gaaaagacat tcatctaacg ctttttcaat tggcttttcc
    54841 atttcatccc ctccattggc gtacatatca tagtatgatt tttatttttt gtcaaaaaac
    54901 acggaataat gtccgcctcc tctgtctaga tttgaaaaaa agaagtagcc atcttcaaag
    54961 aatttcggct tgttttcgac aaaactcctt ctggaacacc gtgaatcaga ggttgtgaaa
    55021 aagtcgttta gttttgagta tgaagaaaaa ttttgtgaaa atggctttcc acatttttgc
    55081 ataaatttgg cttaataccg aaaaactggc ttttgaacac cgtttatatc agaggttgtg
    55141 aaaaagccgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt
    55201 gtcgtttttt gagctaatgc cgatggtcag ctgattgtac accgtttatt gagaggtcgt
    55261 gagcctgccg ttgaactccg agtcacaagg agcaatttca aaaaacagct tctcatgttt
    55321 tatgactatg gtgacttgtg atcgaggagt tggcaggaga atactgttta ttcgtaaaga
    55381 aggggctgtg acaagacttt tgtcacagtc ccttatttcc ttttattata ggatttcgcc
    55441 atttgattgg atgacttgtc tgtaccaatc aaatgatttt ttctttaacc gtttctttgt
    55501 gccattccct tcgtcatctt gatcaacata gatatatcca taacgttttg acatttgtga
    55561 agtagaagcg ctgacgatat caatgcagcc ccacgacgta taaccaaata aatctactcc
    55621 atctttgata gcttccttca tctgctcaat atgctgcttc aaatagtcaa tgcggtaatc
    55681 gtcttgaacg gttccatctt cttctacttt gtctatagct ccgagtccgt tttcagctat
    55741 gaacagtggt ttttgatacc tatcatataa ttgattcaaa gcaattctca aacccttagg
    55801 atcaatctgc cagccccatt cacttgatgg aagatacgga tttttaattc ctccagttat
    55861 taagtttcct tggacccctt cccaattttc tggatgagca ctgactgcac tagacatgta
    55921 gtagctaaaa gaaacaaaat caacagggta ggctgctaag atttcttcat cacctacttc
    55981 tatttttaag ttgacacctt tttcttcaaa cattcgttgc gtatacgctg gataatatcc
    56041 tcttgcttga acatcactaa agaacaaaga agttctttgc tgctcaattg ttttttgaat
    56101 atcatccgga tcacaagaat acggaacaag aagctgatag ctgatcatac aaccgacttg
    56161 tgaacctttg ataatttcat gacaagcttt tgtagctaaa gcacttgcta taaactgatg
    56221 gtgtgcacct tggtatttcg cttgttccaa atggggatgg ttttcttcaa ttactcctac
    56281 gctaacaaaa gggtgatgtt ttacacagtt gatctcattg aaagtcatcc aatattttac
    56341 tttattttta tagcgtttga atactgtttc agcgaaacga acgtaaaact gaataagctt
    56401 tcgattaggc catccaccat attctgtaac caaatataac ggcatttcat aatgggaaag
    56461 agaaacgact ggctcaatcc catattttag acattcgtca aacacttgat cataaaaaaa
    56521 taatcccgct tcattgggtg tttcttcatc accatgagga aaaattcttg tccatgcgat
    56581 agacatccga tagcatttaa aacccatttc agcaaataaa cggatgtctt ctttaaagtg
    56641 atgatagaaa tcatttccgt gtcttttagg ataataatac ttgctgttgg gatcctgcgc
    56701 cgcttttact tgctgatgcg tcatcatatg ccattgatct gtccattttt ctttaggtaa
    56761 atcgctgtcg aatgtataca catcagacac agataatcct ttttcatcta catcccatgc
    56821 accttcacat tgattggccg ctgtcgctcc gccccataaa aatccttctg gaaatttttt
    56881 catatcgatc cccctaaact tgctagtttt cttgtttgat tatttttctt atgactagta
    56941 atgatatttt tgaatctact actatttgta acaataacca ttgtggtcgg atcatatccc
    57001 tctttgatta attcttgcaa atctacttcc atcaataaat cgcctttacg tacttttttt
    57061 ccatcagata ctgcactttg aaagtatttg ccatttaaat tcactgtatc aataccgata
    57121 tggatcagta tctctgctcc ttcttcactg actaccccaa ttgcatgttt cgttggaaaa
    57181 actgttgaaa cttccccgtt tacagaactg tagatttttc cgtccgttgg aatgatcgca
    57241 aacccttctc ctaaagatcc actagcaaat gttggatcgt ttacttcaga taaatcaatt
    57301 atttcacctg ctgcaggagt acttaacact atatcctttg aggattcttc ttcgatttca
    57361 tccttgtagc cgaaaagaaa agttagcata caggaactga tcatcgctaa gaaataacta
    57421 gcaactagcc aaacaaatcc ttcaccaata taaatcggga aagacaaaat acttcttgta
    57481 gcaactgcca aagcaccggc tcctgcggca ccagctactc caccaaatat agctccacat
    57541 atacaagcaa tgtaaaatgg tcgtttatat ttaagagtca caccataaac agcaggttca
    57601 gtgattccaa ataatgctgt gataaatgca gataaagata aactttttac tttcttattt
    57661 ttcgttttca aaaatacccc aagtactgct cctgcttgcg cagctacggc agtcatacat
    57721 gctggaccta gcgtatcacg accgtagaaa gaaagattgt tggtcattac tggtacaagt
    57781 ccccaatgta acccaaaaat cactaacact tgccaaaatc cgccgataac agcaccagat
    57841 aaaacagggc taaagttata aacagctaaa tatccagctg caatcagctg acttgcataa
    57901 tctgcaattg gacctacaac taataaaata attgggacca tcacgatgat acaaatgaat
    57961 ggtgtcaata aatttcgaat actttcgtgc aagcttttgc gaatcttagg ctcaatataa
    58021 gaaagaaccc atacagctaa aattgctgga ataaccgtac ttgtatacct tgctagaatc
    58081 acgggcatcc ctagaaatcg caaggttatg ctatcactat atgcactgac aatcgtagga
    58141 taaacgagag ctgctgcaat aaccatagcg atgaaacgat ctgtattgaa cttttttgca
    58201 gcagtatatg ccaaaaatat aggcaagaaa tagaaaaatg catcagctgc tgcattcaaa
    58261 atgatataag ttcctgattc agtagttaat aaattcaaac tcgtacataa ggcgagtact
    58321 cctttgatca aaccacttcc tgccaagacg cctaaagtcg gggcaaaaac agcggcgatc
    58381 atgtctataa aagagtttaa aatgcctttt ttctcagttc ctctctttcc actagatgca
    58441 tcatcttcta gtttcatttg tttggataat acgtcataca cttcatttac tgcatttcct
    58501 actacgactt gaaacattcc tccagcttcg actacagtga tcacaccttc tagttgctct
    58561 accttctcct tatctgcctt cttattatct tttagggtaa agcgtaacct tgttgcacaa
    58621 tggtaaacat tgctgacatt ttcttcgcct ccgacaaatc ggacaatatc tttcgctaat
    58681 tgtgaatagt ccatttttac ttcccccttg ttaattaatg ccgtagcaat ccttcttaca
    58741 agataagtat agttcttaga aaacggtttc tccattcaat tagtacccct tctaatagcg
    58801 aacgaaactg tacaatagtg tgtatggttt acttagccga gcaaatcact cactgcttga
    58861 aagaaaacaa gtatatcgtt tctcttatag tcaaaggaga caaaaaaaac acgcttgatc
    58921 ttcctttgct gatgtataag tggtatctca agctgatact ctgtgatttt tgtttctact
    58981 tggcttttga taagacagca caacaaagtc tcggcattta acggaagcat aactaggtca
    59041 gaactaggag agattagttg ctgaatggaa tctatctttt tatggatttt tatttgattt
    59101 tcttctttgt agtaatgatc aagcagtttt gttttctttt tttcttcaac tatggtcagc
    59161 ttttcacgca aaatctgata aatctcccaa gattcaaact catctagaac actaggtaga
    59221 taaatcaaat catcccactc aaaaataagc tcttgctcag tagtcaaaat tgcatcatat
    59281 tccgttattt tgtttgattg atttttgaaa agatacactg gttctatccg gcattctttg
    59341 atccacatgt tcatactttg ttgaatctgt ttttttagtg tattcaaaat actaattggc
    59401 tggttactaa caatcagtat ctccccttgt tttcgaaaag gattcaacgc ttcggcaaga
    59461 tacaaaacaa aataagcgct ttcagctaaa ttgggacgta attgaaaatg tattgggaag
    59521 aaacgccgaa ttaaataaat ttctaaagga taacgggaga tgatcttttg tacaaaatga
    59581 ttcatggtat tatgtccggc atccatcctc gttttcattt gtttgatatg ggttagcaaa
    59641 aattctgttt catggaacaa taacgaaaca ggaagatcta aaaaacgaat aatcgtttgc
    59701 tcaaatgctc ttagattctt ctctatttct ttgttttttt catcattaat catcaaataa
    59761 tttaactcta atagtcgatt ttcaattgct tgtttttctt gattggaaaa agaatagggt
    59821 agttctgatg ataacttttt tgtgagggtc tctaccatcg gaataaataa tgtatacctg
    59881 tttgtttttt ctaaaagttc ttctcttcga agaatggtca aaacgagata ccgagaaaat
    59941 cgtatgaagt cctctcctga aacaatataa ttatatgata taagaacctc ttttaatgtc
    60001 ttttggacag tcggcatgat attggaaaaa attgtactga tttgttgagg caaccttgcg
    60061 tgttggatca ccgtttctgt tccaacaaga gataagaaaa ttctttttga attttcttca
    60121 gcaatgattt ttagtccttt tgtcgacgaa acttccagtt ctaatttagg attgcgctcg
    60181 atccaacgca aaatcgtttt tatttgtaat gatacggctg tttttgaaag aaagaaaatg
    60241 tctgctatct tttgcatact gacataccct ttttgaaaca ataagtacgt cacaatagtt
    60301 gaagctcgga aatccatgct ggaatagatt ttatgtttcg tcacctcttg tatgatttgc
    60361 tgtttagttt cagattcaat tgattctaaa aaataacctg aacgtttatt tgactggata
    60421 atgattttct tctcgtagat agcaagcata tttttgattt cagtttggat cgttttcttg
    60481 ttggtaccta aagcttcagc taattcttcg cttgttatcc aatgattctt atttcttaat
    60541 actacaagaa tttggagttg tcgtttattt aagtccattt gcttacacct cctctttgtt
    60601 ataaacatac aggaaaaacc tctcaaatga caatagtcac ttgcagaggt ttaaaaatca
    60661 tttgaagaaa gatctatatt caaggttttt accattagac tggataattt gtttataata
    60721 ttcaaaacta tcttttttca atctcctaaa gcttccgctc ccatcatttt gtctatccac
    60781 ataaataaag ccatatcgct tcctcatttc ccctgttcct gccgatacga tatcgattgg
    60841 tccccaccat aagtaaccaa tgatttggca accatctttt attgcctcag caatttcctg
    60901 aagatgtatt tttagatact cacaacgttt tggatcgtgg attcgattca attcatctgg
    60961 tgcttcatct agtccaatgc cattttcaac tatgaataat ggtaattcat aacggtctgt
    61021 caattcattc atgacataac gaaaccctag aggatcaatt ggccaactcc aaggttcggg
    61081 agatttttcc ttcaaaaagg gattatcgaa accatttgct ccgccagtat ctacgcgcat
    61141 ttctgcttct tttttataga cagcgctacg ataataacta aatgcaatat aatctgccgt
    61201 gttagcttgc aagcactgtt cgtctccatc taacattact ggtgcacagt cttcttcctt
    61261 ccatgtcctg taaatataag ctggataatg ccctcggcac atgacatcgg agaaaaacca
    61321 tgaattgcgt ttaaattgca gtgaaccaaa tacattctct ggatcacagt catatgcata
    61381 agtcgctaat gcagaaaagc tacacataat acccatctta gcatttggat cgattttctt
    61441 taataattga gtagcacggg cgttcgctag aaacatatga tgacaggctt gataaatcac
    61501 tttttttgtt agtgtagcaa tgggttcaag tttatctcta gcagggaatg taaccaatcc
    61561 agcagctgca taaggcatac gaaaagcatt attgatctca ttaaaggtca tccaatattt
    61621 cactttccct tggtagcgtt caaagacggt ctgcgcataa aattcaaaaa agtcgattaa
    61681 ttttcgattt tgccaccccc catactctcc taccaatgct aagggggttt cataatgact
    61741 aagagtaatt actggctcga tatcatatcg atttaatgta ttgattagtc tgtcataaaa
    61801 tacgagacca gcctcatttg gcttctgttc gtctcctctt ggaaatattc gtgcccatga
    61861 gatgcttgtt cgaaaagctt ttaaacctaa ctccttcagt aaacgaatat cctcttcaaa
    61921 tcgatgataa aaatcaatcg cttcatgaga aagataatgg agtgattcgt ctaaagcaat
    61981 ctcccaaatc tttttttctt cattccactg aatactagga tgctggttca tgatcccgat
    62041 aagcgtatct gtcacattag gtaatttgcc gtcttccaac catgcacctt cacattgatt
    62101 agcagctact gctccgcccc ataaaaaatt ttctggaaat atttcctgat ccataactat
    62161 ctctcctcgc tatcaacaat ttcttacttg aagtatagct cgattcatca aaacaagata
    62221 ttctaactat ttcggcagaa gagaagaaaa aaattgaatg ctactcttca tttttaatat
    62281 ttatatataa caaatacata agacgatccc cgaaagaaaa cactcgttaa ccatctaagc
    62341 aaacgattta ttttacttcc aacacaaatg cttcataagg tcgtaaatca actttgccgt
    62401 ctggtaactg tttcagagga taatttgaca caataaccct taaagcttca cgtggaatag
    62461 tgaacgtctg cttttcactg gacatattac aaataacgag ccattgctgg tcttcatata
    62521 tgcgagaata ggcaaagaca tgctcaggcg tcttttccac taattcatag tctccccaga
    62581 cgatgatgtc atgttttcta cgcaaatcga tcaatttctt gtacatataa aaaacagaat
    62641 cttgatctgc taacgcttct tctgcattga tttctttata atttggattg actcgaagcc
    62701 atggaattcc agtagtaaac ccagcatgtt cacgggcgtt ccattgcata ggcgtcctag
    62761 cattgtctct tcctttcgcc ttgattgatt ccaagatttc ctcttttgta aatccagaag
    62821 aaatcctttc gttatacata ttgattgtct caatatcttt tgcttccgcg atatcatcga
    62881 ttgggcaatt cgtcatgcct aactcttctc cttgatagat ataaggggtg cctttcatca
    62941 tatggagcaa gatagcaaac atcttggcac tttcttttcg atacacatcg tcatttcccc
    63001 aacgagaaac aattcgcgga agatcgtgat tattccaaaa taaactattc catccttcat
    63061 cacctaaaga tgtttgccat ttggataata cttttttcag ttcagagata ctcaagggtt
    63121 tcaaatccca tttgtctttg ccttcttgct gatctaatcc tatgtgttcg aattggaaga
    63181 ccatggataa ttcatttctt ttaggattgg aataaagttt agcgatttcg gtagttgctc
    63241 cccatgtttc gccaacagtc atcagatttt tgtcaccaaa agttgcttga ttcatttcct
    63301 gaagatactc atgcagttta ggtccattac ttgtgatttc tttatccggt tctttgccaa
    63361 tcatatcgat cacgtccata cgaaagcccc caataccttt atcgatccag aaattcatca
    63421 tttgataaat ctcttggcga acctgtttat ttttccaatt caaatcaggt tgacgcttgc
    63481 tatacaagtg cagaaaatat tgccccgttt tttcatccaa ctgccaagca gaaccactga
    63541 atattgagcg caatctatta ggctcttttc cattgaccgg atcacgccaa atataatagt
    63601 cccgataagg attttcttta tttttacggg cctcaatgaa ccacggatgt tcatcagaag
    63661 tatgattcac caccagatcc atgatgatac ggatatttct tttctttgct tcttcaatca
    63721 gttcttccat ttcttccatc gtaccgaatt ctgccatgat tgcttgataa ttactgatat
    63781 catacccatt gtcatcattc ggagactcgt atactggaga aagccaaatt gcatcaatac
    63841 ctaaatacgc cagataatcc aacctggaaa taatcccccg caaatctcca ataccatctt
    63901 gatttgaatc ttgaaaactt cttggataga tttgataaac aactgaatgg tgccaccatt
    63961 tttgttccat gattttcctc ctataaatgg atgtttctat tttttgctta atcatttatt
    64021 ttagtctctc tcactaaaaa ttgtaacgct ttcaaatagc tattgcacct tgttatcggt
    64081 aacagaataa aacaaaaaaa cagctgagga aattccctca actgtttttt gaaaggattg
    64141 atttatgcag ggaagaatgc tgatttcaca acttctttat aaacggtgtt cactcagctg
    64201 tcccttggta ttagctcaaa aaacggcaaa atataagaaa ctattttttc gtttttttct
    64261 ctaatactca ggccagaacg ctgatttcac aacctcttta tttcacattc aatatccaca
    64321 tgaatcccac aaatacaaag aacagaccat acatgattgg agatacttct tttcctcttt
    64381 tagctgctaa catggtaatt ggatagaaga taaaacctaa tgcaatccca tctgaaatac
    64441 tataagtcaa aggcatgccg atcaagatca agaatgaagg gatcgcaatc tctaagtttc
    64501 cccattcgat ttgtcgcaaa gattgtgcca tcaatactcc taccataatc aatgctggtg
    64561 cagttacttg tgaagtaaca actgatagta atggagaaaa gaacaagcct aacaagaaga
    64621 aaatccctgt agtaatagct gtgatacctg aacgtcctcc aacggcgata ccagctgatg
    64681 attctacaaa tgcaccaaca ggagaagtac caaataagga acccgcaagc atagctgttg
    64741 aatcagaagc taaagctttc cctacacgag gcatcttgtt gtctttcatg aaacctgctt
    64801 ggtttgctaa tccaactaaa gttcctgctg tatcaaagaa agtcacaagc aagaatgtca
    64861 gcactacgac ccacatttga agagaattga tgtcccctac atgttttaat gcaactaaaa
    64921 atgttggttt caaacttggt gctgcggcaa caatttcaga aggcattgga atcaaacctg
    64981 tgaccagacc taaaatcgtt gtagctgcca tcccgatgaa gatgccacct ggtacacgcc
    65041 gaacaagcaa gatagcagtt acgactaatc caaaaatcgt caaccaagta gaaccaacgt
    65101 tgagtgatcc taatcctact aatgtagatt cattcgaaac gatgatccca ccttcgctca
    65161 gaccaaggaa agcgataaat agaccgatac cgccagaaat cgcatatttc aaatctgctg
    65221 ggatcgcatc aatgatcatt tcccgcagct tgaagattgt gatcaaaata aaaatcaaag
    65281 aagcaacgaa tacgccggct aatgctgttt cccatggaat ccccattcct aaacaaacag
    65341 aataagcaaa aaaagcattg atcccaagag caggtgccgt tccaattgga taacgggcta
    65401 atacccccat taaaacacag ccgatcgcac tggctaatgc agtcgcagta aacactgctc
    65461 cttcatccat tccagaagca cctaatacag ttggatttac aaataagata taagccattg
    65521 agataaatgt tgtaaatcct gccagtacct cacgttttac cgtggtgttc aatttttcta
    65581 tttcgaagta ggaaagtatt ttttccttca tcttccatcc ccctaaatcc gaacattata
    65641 aataaagtcc gttaaaattt cgatttatta ataaaacaac gtgttttatc atatactaaa
    65701 acaaaacata agtaaacaaa aaattcggat tttaagcaaa atccgaacgt tccatctgat
    65761 taaatccctt tatctcaaag ttcatgaaca ttcactttgg attacaagaa tttctttatt
    65821 gtgttaataa agtttcttca ttctagtctc aaaaatcaat agtgtgattt gattttacca
    65881 tttcgtttct gttcttcttt gatgaattct tttgtatcga caggattcag attatttagt
    65941 ccgccaaaac ctgtatcgac catttttccc cagccttttg tcttcttttt tcctgatttg
    66001 agattctttg ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa ataaccttct
    66061 aatcttccca ttagatccac tgtttttcct cctttttatt caatcagacg actcagcttt
    66121 tcctataaca aaataattgc cttcagggtc agaaaaattg aaagtcaaga tgccattgtt
    66181 atctgtgatt tctcctggtt cttccatgtt ctcgtagata gtttcaaatt catccgtaaa
    66241 aaatactaaa gaaggcggtg gtcctaatac ctctggtgaa aacttctgca caaatacttt
    66301 tgggaatacg cctaattcta attcttttga gatgctgagt acaatactct taaattcttc
    66361 tggcaattcg attgtttcag aaatttctgc accaattttt tctacaaaga atcggcaaat
    66421 catctctacg tcattgacat aaagcatcac acgtgttttt tgtatcatca tttgaccctt
    66481 ctttctattt gattttcttt cctgtctata aactgattcc attatatcac gaaagcgcaa
    66541 acaccttgtg tttgatacta ttcaagaaaa ataaagcaat agtttctagg attgctttcc
    66601 tccacaaact ttttttacaa aaaaaaaaac ggcaatgaat gccgctcttc ttatttcgat
    66661 tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca ctagttacgc
    66721 caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca cgtgccgctt
    66781 gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc gtcagcatcg
    66841 ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca tcatagtaga
    66901 tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg aatgattcgc
    66961 caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct tctccgccgc
    67021 aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga ttgattggga
    67081 aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa cgtgcacctt
    67141 ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg ttttggctga
    67201 atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat aattgatgat
    67261 acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat aagatttcga
    67321 tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt ttcatctgtt
    67381 cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg gatcatctcc
    67441 catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa aagcgagcgc
    67501 aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt cttttgcttt
    67561 ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt tttttcgtac
    67621 atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac tcgttcctgt
    67681 atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa aatcggtttc
    67741 tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca agtaatcagc
    67801 taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga tactaatttg
    67861 ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat tcacggcttc
    67921 ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt cgctaacatt
    67981 cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag aaagaccatt
    68041 taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga tttctgctcc
    68101 ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca aacgttcgat
    68161 catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat ctactttgat
    68221 catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta ctaaataaag
    68281 atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag aatctagcgc
    68341 tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc tttattttgt
    68401 ttgtaagact agttcacctc tttttgttgc aaatgcaact attttcaata aaaaaatcaa
    68461 gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa agctcaatca
    68521 caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt tcgcttttct
    68581 gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc attattctca
    68641 ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata atactgaaaa
    68701 agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg agtagtttct
    68761 actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg agacaatcct
    68821 atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc gattagtagg
    68881 accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg atgtccttct
    68941 ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc actgatcagt
    69001 tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc ttttccataa
    69061 tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt cttgttcatt
    69121 ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa gactggttta
    69181 attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa aaaactcgag
    69241 aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa gcttgcatct
    69301 gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg gctgttctta
    69361 ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc tcctaccaac
    69421 tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt gaaattgctc
    69481 cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg ttcaaaagcc
    69541 aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct ttttctaatt
    69601 tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat tcatatagtg
    69661 ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat gcttctttcg
    69721 taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga taatgatcca
    69781 tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt tgccataaca
    69841 cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta tctgctaatt
    69901 cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc actttctttc
    69961 ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc gtcctttctt
    70021 gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat tccttgattt
    70081 catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc ataagatatg
    70141 tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc tcatttattt
    70201 cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt gacaatttct
    70261 tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat ttctgtttca
    70321 ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc aacattgaat
    70381 ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc tgttcctcca
    70441 tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg aatgtttact
    70501 tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa tgcatccacg
    70561 cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt gtcattttca
    70621 gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc tacggaaaca
    70681 ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc tttgaaaggc
    70741 agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc tttgatatca
    70801 tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc ttctgcaata
    70861 gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc gacacttgtt
    70921 tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg aagcatttct
    70981 aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt ttttgttact
    71041 tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt ttcctttgat
    71101 cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt aatcgatccc
    71161 ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc tcatcagttt
    71221 tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat attggatggc
    71281 ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa gtttcgctaa
    71341 tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct ctgattgaat
    71401 cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt ttttcggttc
    71461 tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt aaaatgcttt
    71521 caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat ctacatcggt
    71581 tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag cgggcaataa
    71641 tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc cgccaactgc
    71701 gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac caggtaaaat
    71761 catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa tcgttgtcgt
    71821 aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac aatctaaggc
    71881 agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca cgatttcacc
    71941 taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt ggatggcttg
    72001 taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga atggttcgcc
    72061 tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag acaaataagt
    72121 atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact ttttattttc
    72181 gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga tcgtacaaaa
    72241 atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt gcgctactgc
    72301 cggcatagca atggcgaaac aagctccgat aaaatcaccg accaagatca atacgatccc
    72361 tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata aaattgcttg
    72421 accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat tgaatacaaa
    72481 aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca ttgcttgacc
    72541 attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag taaagatttt
    72601 cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca cttgaaatcc
    72661 taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct gcagtagtag
    72721 acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat tttcgatgat
    72781 aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat aactccctta
    72841 tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat atttttcaaa
    72901 tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg aaaattttct
    72961 gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca atggtccagt
    73021 ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc tgcaaaacta
    73081 cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt tgttctcccc
    73141 cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc ttcgatattt
    73201 gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat tagttctttc
    73261 atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat ctttactgga
    73321 tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct gactcctaat
    73381 ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc aatcacgata
    73441 tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc ttcgacaatg
    73501 ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt agtggctttt
    73561 tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg gatctccctt
    73621 ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata gatgattcga
    73681 tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa ctccgtcgat
    73741 tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa gtcttctata
    73801 accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt atttcgataa
    73861 ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa ttcgatttct
    73921 tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag attctctgct
    73981 aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata aacataattc
    74041 aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa agctatctcg
    74101 tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg agtgactgcg
    74161 cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat gacttggata
    74221 tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc gaataaagct
    74281 tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat caattggatc
    74341 gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc attgaacgtc
    74401 tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa ttgaaattcc
    74461 tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt atttttccaa
    74521 ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa tgtaaaaagc
    74581 aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga atagatctcc
    74641 tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg agccggtgtt
    74701 aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt gataaaatca
    74761 tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg ctttccataa
    74821 ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat atcttcatct
    74881 aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat cgggattcgg
    74941 ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa aaagccgtct
    75001 ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag cttcaacaaa
    75061 aaacctttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa ccagatattc
    75121 gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact tgctagatgt
    75181 tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat tgttcttgtt
    75241 ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat aataaaaagt
    75301 agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa aataagccat
    75361 atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa attttgccca
    75421 tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacaactaaa
    75481 ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat gcaagctttt
    75541 aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga taagaagcga
    75601 ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat aggctatctc
    75661 tggcacaccg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca actccgttca
    75721 taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc catctttttt
    75781 ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt gtcttacttc
    75841 ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg aaatcaattc
    75901 ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga atagttattt
    75961 ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc tgatgttcgg
    76021 caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct tcccaagcgg
    76081 attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt tctttagccg
    76141 ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt ttattcttga
    76201 cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga aatcccgttt
    76261 gctcataact agaaggacct gtacctggac cataataccc ataagaatct ctgccgtaag
    76321 aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct ctgcccgaac
    76381 ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct tgggtcggca
    76441 gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt tttttcattg
    76501 tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc taaaaggaag
    76561 agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt agtcattgag
    76621 taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca gcatgaacaa
    76681 gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct gacaaaagtg
    76741 caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat gagcaagttt
    76801 ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt catctagcga
    76861 actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat tatctagctc
    76921 cttagctgct tgaattgcaa gcctgatatg cggataccct aatatcgctt cttcatatgc
    76981 accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt tatgacgata
    77041 ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg ataaagaaaa
    77101 aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca ttgcccaaat
    77161 cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt ccgcttttcg
    77221 cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc atgctatttg
    77281 agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata aagcatagtc
    77341 catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg gacttaatgt
    77401 tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca ctgctttttt
    77461 tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc tgaaaccttt
    77521 tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac aacttgccgt
    77581 cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac tatctttaaa
    77641 caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat aagcataaac
    77701 agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt tggaagcgcc
    77761 tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat tcaagtaagg
    77821 tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag aaaagaacgc
    77881 gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt gatagacttt
    77941 ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa accgatctaa
    78001 taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga acagatacca
    78061 tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt tattagcaaa
    78121 tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca gcatcataac
    78181 ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa agcttgcagt
    78241 atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag ccgtcgtcca
    78301 tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc aataagacaa
    78361 atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg gtgctgtgta
    78421 tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt ggctgtttgc
    78481 caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata ctttctggaa
    78541 gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc tctttttctt
    78601 tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag aatttttttc
    78661 tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac tacttgggta
    78721 tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa ttcaatcggt
    78781 gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg aatcccagtc
    78841 gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa tatgtttcta
    78901 gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct atacgattct
    78961 tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga gatttctgtt
    79021 gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc tgcgtgatcc
    79081 gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca ttcaggcaag
    79141 gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg aatcattttt
    79201 tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac acgtccgaat
    79261 gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt attgcggaca
    79321 tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc tgaagcagct
    79381 tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata attattgata
    79441 tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac cgaacgtttt
    79501 gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt attgtttaaa
    79561 gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat agcatttcct
    79621 gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc agcagcacta
    79681 ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc ttggcttggt
    79741 acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc ttcgtaaacg
    79801 tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc gtttcgtaca
    79861 cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc ttctatgaat
    79921 ttacttgatt taccataata cacagtacta atagaagaag ttggattttt catgccagtt
    79981 agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc ttctgctact
    80041 tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta atcgtcaatg
    80101 gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc tgagctttca
    80161 ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt gattgcttct
    80221 acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg agatcctaat
    80281 gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc ttcttgttca
    80341 ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa ataactcatt
    80401 aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat caaaccgatg
    80461 actggtacat attgttttag agcaatgatt acatttccga tttctgaaat agataacagt
    80521 ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac tggaaagatc
    80581 tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat ttttgcaccg
    80641 gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac tactgcttgt
    80701 ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg gacgattcct
    80761 tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc acgtccattg
    80821 tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt tttcatctga
    80881 aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag caccgaagtc
    80941 attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta atacattgtt
    81001 ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt ctgttttttc
    81061 tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat ggattttctt
    81121 attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc tctccccttt
    81181 ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc tgggtaaatg
    81241 gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat tggtggttca
    81301 tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa taatgaacgt
    81361 ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc ggctgcacgc
    81421 aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc gccaattgct
    81481 gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc aattccttct
    81541 tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt ggctaagcct
    81601 gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt tttatttgaa
    81661 ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg atcatcattg
    81721 tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt atgtcccatg
    81781 ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga gtttccttcg
    81841 taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc tgcagcttgt
    81901 gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac atctgggcga
    81961 gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct ttcaacccaa
    82021 cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt cttcgcgatt
    82081 ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat agctgctgta
    82141 ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat tcctttgatt
    82201 gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg tgtgaataaa
    82261 gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc accagggata
    82321 tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat accatctttg
    82381 aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt gtacaagttg
    82441 ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa atcgatgaat
    82501 aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt gccgtcttca
    82561 atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat cttgcttgcg
    82621 attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat gatcaaatgc
    82681 agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa acttgcttgt
    82741 ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt ttccattact
    82801 tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat tttttccaat
    82861 cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc cataattagt
    82921 catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc tagattgatc
    82981 gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa agtattgccg
    83041 gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat catttggttc
    83101 cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc tgcgtcctga
    83161 gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag atggacagac
    83221 tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg agatggcaca
    83281 gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc caacgtaacg
    83341 gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc atttgacaga
    83401 taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc gcttctcgtc
    83461 acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat taagactgcg
    83521 cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc aacagctgca
    83581 caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt tttttccatt
    83641 tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac cgtatcttga
    83701 tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg ttccatgtac
    83761 atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg aaataagaaa
    83821 cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat cttagtcaat
    83881 ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg tatggatggg
    83941 atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg aaacaaaaaa
    84001 tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc gtgcgatcaa
    84061 acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa acattgagac
    84121 gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca cattagaaga
    84181 aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca tattgtattt
    84241 agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa agcatcctga
    84301 tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc ctgtactgta
    84361 catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag aacctgttta
    84421 tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat tagaagatat
    84481 ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata cactggatgc
    84541 agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg atcctgcgac
    84601 attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag tttcttggtc
    84661 ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct tgacacgaac
    84721 aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga ttgcaaaaac
    84781 gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa ttcttgtcgc
    84841 agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata agccgaaatt
    84901 tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctaaacatt
    84961 tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta aaacttccat
    85021 caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata gagatccaat
    85081 gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa attccgccaa
    85141 gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt gcatcatcat
    85201 agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt ttttgatgtc
    85261 tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa ttgtatgttt
    85321 cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc tttcttattg
    85381 attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt agttgctaat
    85441 caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg cctgtgttgg
    85501 tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt ttttgatttt
    85561 agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg ctccagaaaa
    85621 ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct ccgtattctt
    85681 agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta ccgaaaaaga
    85741 tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga taaccgtaaa
    85801 tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc ttcaatctat
    85861 tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa tcagtttgca
    85921 aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac aatcagtcag
    85981 tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg attttctagc
    86041 cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca atcagcgttt
    86101 agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc ttggaaaaga
    86161 gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata acaactattt
    86221 tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa acctcagtca
    86281 aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct ttatcaatac
    86341 gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca gcacactctc
    86401 ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt atcttaaaga
    86461 cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca acaataacta
    86521 tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc tcgaaacgtt
    86581 tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat ctctgactga
    86641 taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg aagcattgga
    86701 ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact tgtatcaaca
    86761 gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac caaattactt
    86821 tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac cgaatttgtt
    86881 caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc agcaggaatt
    86941 aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg atctttccac
    87001 tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt ctctagatat
    87061 ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt tgactgaact
    87121 cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct gttttttctt
    87181 ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag aatagaagaa
    87241 tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta ccgatcagct
    87301 gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata cctaatgaaa
    87361 gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac aactctctgc
    87421 aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg gtttgctgta
    87481 agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc agtagggaga
    87541 catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga tctcttggtg
    87601 ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat aaagtgattg
    87661 tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg cgattcagca
    87721 taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag aaattgatgg
    87781 ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca atcgtatgga
    87841 aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta cctttgattc
    87901 ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg ccgtctccgg
    87961 tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt ttatgttctg
    88021 gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct tcatcagaca
    88081 taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca atttgttccg
    88141 caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag atccctgctt
    88201 gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc aatccggcga
    88261 tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc gaatctactt
    88321 ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca gctgtctgac
    88381 tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact tctggtgcac
    88441 ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata tatttgtatt
    88501 cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct gtttctgtgt
    88561 gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc tgttcttctt
    88621 ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct tcttttgcag
    88681 ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct gaacagatca
    88741 ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc ttttcttttg
    88801 ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat ccttcgggta
    88861 ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc ataccatatc
    88921 gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga aaaatttgtt
    88981 tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc gattgcaatg
    89041 cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca ccgactgcag
    89101 aaccagattg tacaagcgtt cctgaaaaac ccatattttt ctggtcgccg atcgattctt
    89161 cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc aaaatcgatt
    89221 cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg acatctccgg
    89281 cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt tcttgcatcc
    89341 cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc aatgattctt
    89401 ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg aataaaataa
    89461 tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg atcaacacga
    89521 tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc tgctgccttg
    89581 cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct tgcgacaagc
    89641 cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg taccagttca
    89701 tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt tttcaggcgt
    89761 atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct gggcctaact
    89821 gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc agataaccaa
    89881 taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa ttgaagtaaa
    89941 aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct tttctttaat
    90001 aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac tcgttgtcat
    90061 gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta gacaaagacc
    90121 ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt tcttagaaag
    90181 cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa tcctgctagt
    90241 actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta gattgacaat
    90301 tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac tttcttttaa
    90361 atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta tatagaaaaa
    90421 gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg gatcacttgt
    90481 ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa ccataggaaa
    90541 tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc caaaattttc
    90601 tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac ccaataagat
    90661 aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt caccaaacca
    90721 cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc cgcttatcaa
    90781 agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta attccgcaag
    90841 tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc actttttgcc
    90901 taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta aaaataaatc
    90961 acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa cgagaataac
    91021 agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg ttccaattgc
    91081 gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca ttgccggttt
    91141 ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt tcccaagaag
    91201 gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg atggctgtga
    91261 ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa aatccgattg
    91321 ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc tctttttttc
    91381 caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa actgcgatcc
    91441 ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc atcaaaaatg
    91501 cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata atgacagact
    91561 gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa ttgaattttg
    91621 gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct gaggcccaag
    91681 cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc acaataagat
    91741 gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta ttcaattcca
    91801 tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg acgaaaaata
    91861 taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt tccaatcgag
    91921 tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc gcaaaataaa
    91981 caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa ctgcctaatg
    92041 ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag agcatcatcg
    92101 tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt gccatatcaa
    92161 atttcaaatt caaaaaagtg atgatccaat aaacgactaa aatgacaact aatgtaaatt
    92221 tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga gcgagtgctg
    92281 aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag acaacgaacc
    92341 cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt tggggaaaca
    92401 ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat actgcaacca
    92461 gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt cgtacacttg
    92521 ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt gatgaaccag
    92581 tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt cttttatttt
    92641 tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag tatcaccgaa
    92701 acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg atccaactta
    92761 tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca gggggaaata
    92821 caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt tattctttca
    92881 cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc tttggcgatt
    92941 ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat gatagccgca
    93001 cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc cattctttca
    93061 acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg agaagtaata
    93121 aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat tttttcattt
    93181 aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca attgaccata
    93241 ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac agtaaaagtc
    93301 agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc aatcaattga
    93361 ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc ggcggcggtt
    93421 gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc ctttactgat
    93481 ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt atgtttgatt
    93541 acgattcctc cagcagcata agggacatag cccatcttat gaggatctat cgtgacagaa
    93601 tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt tacgcagtgg
    93661 tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac aaattctcct
    93721 gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac atgtaaatag
    93781 aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc tacactgtct
    93841 acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg gattttttgt
    93901 ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata atcctcttga
    93961 actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc cttcatccaa
    94021 gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt ttttccgcta
    94081 cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc ggtcatttcc
    94141 aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt cactttttca
    94201 ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta ccataatcct
    94261 tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt catattgaat
    94321 aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat agaagattcc
    94381 aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa agcaggcatc
    94441 aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg gatcgatcct
    94501 gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg ggtggctaga
    94561 taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg aatataattt
    94621 tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata aactggtcca
    94681 ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat ttttcttccc
    94741 tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac acctcttcac
    94801 tatagcgcca aaaatgagaa tttttttttt ataacatcaa atccacgtag atttttacaa
    94861 acaaaaataa ataa
//
LOCUS       DAJSMI010000002        53616 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJSMI010000002 DAJSMI010000000
VERSION     DAJSMI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.002"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            33..1664
                     /locus_tag="ON430_000081"
     CDS             33..1664
                     /locus_tag="ON430_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCR3218033.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1726..2367)
                     /locus_tag="ON430_000082"
     CDS             complement(1726..2367)
                     /locus_tag="ON430_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR3218034.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2553..2629
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2649..2957
                     /gene="rplU"
                     /locus_tag="ON430_000083"
     CDS             2649..2957
                     /gene="rplU"
                     /locus_tag="ON430_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR3218035.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2984..3328
                     /locus_tag="ON430_000084"
     CDS             2984..3328
                     /locus_tag="ON430_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCR3218036.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3341..3634
                     /gene="rpmA"
                     /locus_tag="ON430_000085"
     CDS             3341..3634
                     /gene="rpmA"
                     /locus_tag="ON430_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR3218037.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4068..6875
                     /locus_tag="ON430_000086"
     CDS             4068..6875
                     /locus_tag="ON430_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3218038.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7066..7557
                     /locus_tag="ON430_000087"
     CDS             7066..7557
                     /locus_tag="ON430_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3218039.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7660..8649
                     /gene="manX"
                     /locus_tag="ON430_000088"
     CDS             7660..8649
                     /gene="manX"
                     /locus_tag="ON430_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR3218040.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8682..9485
                     /locus_tag="ON430_000089"
     CDS             8682..9485
                     /locus_tag="ON430_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR3218041.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9506..10420
                     /locus_tag="ON430_000090"
     CDS             9506..10420
                     /locus_tag="ON430_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR3218042.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10503..10877
                     /locus_tag="ON430_000091"
     CDS             10503..10877
                     /locus_tag="ON430_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCR3218043.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11016..11261
                     /locus_tag="ON430_000092"
     CDS             11016..11261
                     /locus_tag="ON430_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3218044.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11433..12497
                     /locus_tag="ON430_000093"
     CDS             11433..12497
                     /locus_tag="ON430_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR3218045.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12743..13168
                     /locus_tag="ON430_000094"
     CDS             12743..13168
                     /locus_tag="ON430_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3218046.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13161..13610
                     /gene="nusB"
                     /locus_tag="ON430_000095"
     CDS             13161..13610
                     /gene="nusB"
                     /locus_tag="ON430_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR3218047.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13707..14561
                     /locus_tag="ON430_000096"
     CDS             13707..14561
                     /locus_tag="ON430_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCR3218048.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14562..15899
                     /locus_tag="ON430_000097"
     CDS             14562..15899
                     /locus_tag="ON430_000097"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR3218049.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15904..16128
                     /locus_tag="ON430_000098"
     CDS             15904..16128
                     /locus_tag="ON430_000098"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR3218050.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16130..17020
                     /locus_tag="ON430_000099"
     CDS             16130..17020
                     /locus_tag="ON430_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR3218051.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17029..17847
                     /locus_tag="ON430_000100"
     CDS             17029..17847
                     /locus_tag="ON430_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCR3218052.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17954..18403
                     /locus_tag="ON430_000101"
     CDS             17954..18403
                     /locus_tag="ON430_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR3218053.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18421..20103
                     /gene="recN"
                     /locus_tag="ON430_000102"
     CDS             18421..20103
                     /gene="recN"
                     /locus_tag="ON430_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR3218054.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20158..20274)
                     /locus_tag="ON430_000103"
     CDS             complement(20158..20274)
                     /locus_tag="ON430_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCR3218055.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20347..21303)
                     /locus_tag="ON430_000104"
     CDS             complement(20347..21303)
                     /locus_tag="ON430_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3218056.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21474..21842
                     /locus_tag="ON430_000105"
     CDS             21474..21842
                     /locus_tag="ON430_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCR3218057.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22012..22443
                     /gene="mraZ"
                     /locus_tag="ON430_000106"
     CDS             22012..22443
                     /gene="mraZ"
                     /locus_tag="ON430_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR3218058.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22464..23423
                     /gene="rsmH"
                     /locus_tag="ON430_000107"
     CDS             22464..23423
                     /gene="rsmH"
                     /locus_tag="ON430_000107"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR3218059.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23428..23829
                     /gene="ftsL"
                     /locus_tag="ON430_000108"
     CDS             23428..23829
                     /gene="ftsL"
                     /locus_tag="ON430_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR3218060.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23830..26022
                     /locus_tag="ON430_000109"
     CDS             23830..26022
                     /locus_tag="ON430_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR3218061.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26048..27010
                     /locus_tag="ON430_000110"
     CDS             26048..27010
                     /locus_tag="ON430_000110"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR3218062.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27024..28385
                     /locus_tag="ON430_000111"
     CDS             27024..28385
                     /locus_tag="ON430_000111"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR3218063.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28411..29499
                     /gene="murG"
                     /locus_tag="ON430_000112"
     CDS             28411..29499
                     /gene="murG"
                     /locus_tag="ON430_000112"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR3218064.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29514..30734
                     /locus_tag="ON430_000113"
     CDS             29514..30734
                     /locus_tag="ON430_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCR3218065.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30890..32215
                     /gene="ftsA"
                     /locus_tag="ON430_000114"
     CDS             30890..32215
                     /gene="ftsA"
                     /locus_tag="ON430_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR3218066.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32238..33479
                     /gene="ftsZ"
                     /locus_tag="ON430_000115"
     CDS             32238..33479
                     /gene="ftsZ"
                     /locus_tag="ON430_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR3218067.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33496..34173
                     /locus_tag="ON430_000116"
     CDS             33496..34173
                     /locus_tag="ON430_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCR3218068.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34187..34786
                     /locus_tag="ON430_000117"
     CDS             34187..34786
                     /locus_tag="ON430_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCR3218069.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34801..35088
                     /locus_tag="ON430_000118"
     CDS             34801..35088
                     /locus_tag="ON430_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCR3218070.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35137..35919
                     /locus_tag="ON430_000119"
     CDS             35137..35919
                     /locus_tag="ON430_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR3218071.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35940..36647
                     /locus_tag="ON430_000120"
     CDS             35940..36647
                     /locus_tag="ON430_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCR3218072.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36909..39707
                     /gene="ileS"
                     /locus_tag="ON430_000121"
     CDS             36909..39707
                     /gene="ileS"
                     /locus_tag="ON430_000121"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR3218073.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39756..41282)
                     /locus_tag="ON430_000122"
     CDS             complement(39756..41282)
                     /locus_tag="ON430_000122"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR3218074.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41297..41944)
                     /locus_tag="ON430_000123"
     CDS             complement(41297..41944)
                     /locus_tag="ON430_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCR3218075.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42128..42457)
                     /locus_tag="ON430_000124"
     CDS             complement(42128..42457)
                     /locus_tag="ON430_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCR3218076.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42634..43362
                     /gene="sfsA"
                     /locus_tag="ON430_000125"
     CDS             42634..43362
                     /gene="sfsA"
                     /locus_tag="ON430_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR3218077.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43378..44391
                     /locus_tag="ON430_000126"
     CDS             43378..44391
                     /locus_tag="ON430_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3218078.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44391..45668
                     /locus_tag="ON430_000127"
     CDS             44391..45668
                     /locus_tag="ON430_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3218079.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45731..48433
                     /locus_tag="ON430_000128"
     CDS             45731..48433
                     /locus_tag="ON430_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3218080.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48585..48902
                     /locus_tag="ON430_000129"
     CDS             48585..48902
                     /locus_tag="ON430_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3218081.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48932..49252
                     /locus_tag="ON430_000130"
     CDS             48932..49252
                     /locus_tag="ON430_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3218082.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49360..50820
                     /locus_tag="ON430_000131"
     CDS             49360..50820
                     /locus_tag="ON430_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3218083.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50888..51109
                     /locus_tag="ON430_000132"
     CDS             50888..51109
                     /locus_tag="ON430_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218084.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51140..51322
                     /locus_tag="ON430_000133"
     CDS             51140..51322
                     /locus_tag="ON430_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCR3218085.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51322..51735
                     /locus_tag="ON430_000134"
     CDS             51322..51735
                     /locus_tag="ON430_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCR3218086.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51858..53039
                     /locus_tag="ON430_000135"
     CDS             51858..53039
                     /locus_tag="ON430_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3218087.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53110..53274)
                     /locus_tag="ON430_000136"
     CDS             complement(53110..53274)
                     /locus_tag="ON430_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218088.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53428..53501
                     /locus_tag="ON430_000137"
     tRNA            53428..53501
                     /locus_tag="ON430_000137"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53462..53464,aa:Arg,seq:cct)
BASE COUNT        17699 a         9100 c        11601 g        15216 t
ORIGIN      
        1 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
       61 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
      121 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
      181 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
      241 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
      301 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
      361 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
      421 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
      481 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
      541 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
      601 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
      661 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
      721 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
      781 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
      841 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
      901 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
      961 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
     1021 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
     1081 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
     1141 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
     1201 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
     1261 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
     1321 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
     1381 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
     1441 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
     1501 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
     1561 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
     1621 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
     1681 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
     1741 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
     1801 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
     1861 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
     1921 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
     1981 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
     2041 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
     2101 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
     2161 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
     2221 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
     2281 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
     2341 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
     2401 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
     2461 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
     2521 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
     2581 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
     2641 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
     2701 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
     2761 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
     2821 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
     2881 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
     2941 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
     3001 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
     3061 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
     3121 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
     3181 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
     3241 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
     3301 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
     3361 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
     3421 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
     3481 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
     3541 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
     3601 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
     3661 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
     3721 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
     3781 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
     3841 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
     3901 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
     3961 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
     4021 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
     4081 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
     4141 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
     4201 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
     4261 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
     4321 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
     4381 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
     4441 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
     4501 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
     4561 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
     4621 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
     4681 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
     4741 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
     4801 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
     4861 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
     4921 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
     4981 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
     5041 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
     5101 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
     5161 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
     5221 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
     5281 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
     5341 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
     5401 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
     5461 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
     5521 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
     5581 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
     5641 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
     5701 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
     5761 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
     5821 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
     5881 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
     5941 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
     6001 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
     6061 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
     6121 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
     6181 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
     6241 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
     6301 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
     6361 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
     6421 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
     6481 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
     6541 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
     6601 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
     6661 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
     6721 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
     6781 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
     6841 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
     6901 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
     6961 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
     7021 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
     7081 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
     7141 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
     7201 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
     7261 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
     7321 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
     7381 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
     7441 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
     7501 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
     7561 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
     7621 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
     7681 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
     7741 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
     7801 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
     7861 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
     7921 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
     7981 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
     8041 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
     8101 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
     8161 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
     8221 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
     8281 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
     8341 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
     8401 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
     8461 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
     8521 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
     8581 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
     8641 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
     8701 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
     8761 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
     8821 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
     8881 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
     8941 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
     9001 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
     9061 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
     9121 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
     9181 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
     9241 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
     9301 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
     9361 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
     9421 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
     9481 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
     9541 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
     9601 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
     9661 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
     9721 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
     9781 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
     9841 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
     9901 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
     9961 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    10021 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    10081 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    10141 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    10201 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    10261 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    10321 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    10381 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    10441 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    10501 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    10561 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    10621 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    10681 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    10741 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    10801 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    10861 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    10921 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    10981 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    11041 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    11101 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    11161 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    11221 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    11281 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    11341 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    11401 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    11461 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    11521 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    11581 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    11641 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    11701 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    11761 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    11821 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    11881 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    11941 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    12001 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    12061 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    12121 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    12181 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    12241 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    12301 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    12361 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    12421 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    12481 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    12541 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    12601 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    12661 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    12721 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    12781 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    12841 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    12901 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    12961 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    13021 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    13081 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    13141 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    13201 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    13261 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    13321 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    13381 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    13441 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    13501 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    13561 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    13621 ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa
    13681 cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg
    13741 aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc
    13801 ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata
    13861 aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga
    13921 tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg
    13981 gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta
    14041 tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg
    14101 gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt
    14161 ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac
    14221 cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg
    14281 aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac
    14341 attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc
    14401 gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag
    14461 gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc
    14521 aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa
    14581 ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac
    14641 gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt
    14701 tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa
    14761 aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat
    14821 atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg
    14881 ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag
    14941 ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg
    15001 gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg
    15061 ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc
    15121 agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga
    15181 ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata
    15241 cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag
    15301 atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag
    15361 agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc
    15421 agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa
    15481 gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta
    15541 cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac
    15601 aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac
    15661 gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg
    15721 ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga
    15781 cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga
    15841 tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa
    15901 aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag
    15961 ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa
    16021 ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg
    16081 acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac
    16141 tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga
    16201 gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa
    16261 aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt
    16321 tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga
    16381 tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt
    16441 attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag cattccagct
    16501 aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt
    16561 ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa
    16621 aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg
    16681 ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact
    16741 tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt
    16801 gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa
    16861 tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt
    16921 atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct
    16981 gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa
    17041 cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt
    17101 tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa
    17161 aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga
    17221 gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca
    17281 ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca
    17341 aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat
    17401 cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc
    17461 ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg
    17521 cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa
    17581 ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat
    17641 caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa
    17701 ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg
    17761 aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat
    17821 gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc
    17881 ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag
    17941 gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga
    18001 gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt
    18061 tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc
    18121 agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt
    18181 ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt
    18241 gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa
    18301 agaattattt tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga
    18361 ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg
    18421 atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt
    18481 cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat
    18541 gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa
    18601 aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg
    18661 gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta
    18721 actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga
    18781 atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct
    18841 gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag
    18901 agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag
    18961 caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata
    19021 gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt
    19081 agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat
    19141 gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat
    19201 ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa
    19261 gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta
    19321 gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc
    19381 attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt
    19441 tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag
    19501 caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc
    19561 attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta
    19621 aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg
    19681 accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct
    19741 cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt
    19801 tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt
    19861 tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca
    19921 gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct
    19981 gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt
    20041 gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc
    20101 tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta
    20161 catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca
    20221 aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt
    20281 caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg
    20341 gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa
    20401 tgttaataga actagccata taaaggcatc atccctttca gcaataggta acttaatgtt
    20461 cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa
    20521 aattttcata atattattca gattattcga tacgattgct gaaaaagtct gattcatttg
    20581 ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac
    20641 atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc
    20701 tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat
    20761 atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt
    20821 atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat
    20881 gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa
    20941 gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt
    21001 caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc
    21061 aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc
    21121 tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa
    21181 ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt
    21241 ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat
    21301 catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat
    21361 ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg
    21421 tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca
    21481 cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc
    21541 aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg
    21601 cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta
    21661 tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag
    21721 catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt
    21781 ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat
    21841 gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa
    21901 aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt
    21961 gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg
    22021 ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt
    22081 gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat
    22141 ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa
    22201 gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa
    22261 caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc
    22321 gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt
    22381 tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta
    22441 taagaatagg aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga
    22501 aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg
    22561 gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg
    22621 cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg
    22681 aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg
    22741 agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct cctcagctag
    22801 atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc
    22861 agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa
    22921 agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac
    22981 gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag
    23041 tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg
    23101 tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc
    23161 ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa
    23221 ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg
    23281 tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt
    23341 cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa
    23401 aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa
    23461 tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat
    23521 caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa
    23581 aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg
    23641 ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat
    23701 gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa
    23761 cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa
    23821 gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat
    23881 ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattattc
    23941 tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca
    24001 ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga
    24061 acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa
    24121 gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat
    24181 tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg
    24241 aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg
    24301 aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc
    24361 gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct
    24421 cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt
    24481 ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa
    24541 gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga
    24601 caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat
    24661 caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc
    24721 ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg
    24781 actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca
    24841 accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa
    24901 accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt
    24961 gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc
    25021 atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga
    25081 caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt
    25141 gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg
    25201 aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa
    25261 gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct
    25321 ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa
    25381 aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaacc
    25441 ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg
    25501 tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg
    25561 aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa
    25621 cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa
    25681 gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa
    25741 agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc
    25801 aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg
    25861 atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt
    25921 attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa
    25981 aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga
    26041 aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat
    26101 tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg
    26161 tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt
    26221 gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac
    26281 tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga
    26341 tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat
    26401 tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg
    26461 tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct
    26521 cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt
    26581 tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca
    26641 gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta
    26701 caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact
    26761 tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat
    26821 ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa
    26881 acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg
    26941 gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat
    27001 cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc
    27061 ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg
    27121 cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc
    27181 tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat
    27241 tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc
    27301 aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc
    27361 caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac
    27421 tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag
    27481 caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct
    27541 tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg
    27601 aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc
    27661 aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag
    27721 aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag
    27781 acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat
    27841 ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag
    27901 caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg
    27961 taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa
    28021 aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg
    28081 tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa
    28141 aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa
    28201 aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag
    28261 cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg
    28321 atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa
    28381 aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca
    28441 gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat
    28501 gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca
    28561 ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac
    28621 atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat
    28681 tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct
    28741 gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc
    28801 aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc
    28861 ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat
    28921 actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg
    28981 atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt
    29041 gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag
    29101 atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat
    29161 atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc
    29221 gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat
    29281 gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca
    29341 gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa
    29401 gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa
    29461 cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta
    29521 agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt
    29581 ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa
    29641 aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag
    29701 aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc
    29761 aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa
    29821 gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac
    29881 cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat
    29941 caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag
    30001 aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac
    30061 agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg
    30121 taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg
    30181 aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg
    30241 aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat
    30301 cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc
    30361 tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga
    30421 accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca
    30481 tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc
    30541 aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag
    30601 aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt
    30661 caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt
    30721 taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa
    30781 tttatggtag aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg
    30841 ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac
    30901 aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata
    30961 tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg
    31021 aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc
    31081 agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct
    31141 tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga
    31201 tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca
    31261 aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc
    31321 tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac
    31381 gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt
    31441 gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac
    31501 gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt
    31561 gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt
    31621 attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc
    31681 agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt
    31741 gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa
    31801 caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt
    31861 gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt
    31921 aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt
    31981 aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc
    32041 tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac
    32101 atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc
    32161 cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat
    32221 cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa
    32281 tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa
    32341 acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa
    32401 aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc
    32461 agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag
    32521 acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg
    32581 caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac
    32641 gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt
    32701 tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag
    32761 ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg
    32821 tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg
    32881 tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag
    32941 aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta
    33001 ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg
    33061 aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc
    33121 taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag
    33181 gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc
    33241 aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag
    33301 aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg
    33361 atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa
    33421 caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa
    33481 ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat
    33541 tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct
    33601 gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac
    33661 cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg
    33721 catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac
    33781 tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa
    33841 ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca
    33901 gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg
    33961 ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg
    34021 ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa
    34081 ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc
    34141 cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa
    34201 cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga
    34261 ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca
    34321 atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg
    34381 tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca
    34441 atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa
    34501 agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc
    34561 gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga
    34621 aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga
    34681 tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag
    34741 ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg
    34801 atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt
    34861 atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga
    34921 cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg
    34981 attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa
    35041 ggtttagcac agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa
    35101 aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac
    35161 tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa
    35221 ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa
    35281 acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca
    35341 gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa
    35401 acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc
    35461 cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac
    35521 ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta
    35581 acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg
    35641 ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg
    35701 atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa
    35761 gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact attagatatt
    35821 atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa
    35881 gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca
    35941 tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg cggggatata
    36001 atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc
    36061 aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca
    36121 atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag
    36181 taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag
    36241 atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa
    36301 aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg
    36361 aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc
    36421 aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg
    36481 tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg
    36541 atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac
    36601 aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac
    36661 tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa
    36721 agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg
    36781 ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc
    36841 atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt
    36901 atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg
    36961 gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc
    37021 aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat
    37081 atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca
    37141 ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg
    37201 gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg
    37261 cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag
    37321 atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt
    37381 cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc tatatttaca
    37441 aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa
    37501 ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca
    37561 aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc
    37621 cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg
    37681 gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg
    37741 aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc
    37801 atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg
    37861 gtactggatt agtccatact gctccaggac atggggaaga tgactatatc gctggtagca
    37921 aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac
    37981 ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag
    38041 aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga
    38101 cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc
    38161 gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac
    38221 gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg
    38281 gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa
    38341 tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag
    38401 cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa
    38461 aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac
    38521 gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc
    38581 gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca
    38641 aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt
    38701 taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct
    38761 tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc
    38821 aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag
    38881 aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat
    38941 atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat
    39001 ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat
    39061 tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc
    39121 gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta
    39181 tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc
    39241 agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct
    39301 ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca
    39361 gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc
    39421 gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact
    39481 actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg
    39541 ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta
    39601 aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg
    39661 aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg
    39721 gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca
    39781 cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa
    39841 gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc
    39901 caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag
    39961 aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta
    40021 ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct
    40081 tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc
    40141 gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat
    40201 acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt
    40261 ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg
    40321 cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc
    40381 ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac
    40441 acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt
    40501 tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata
    40561 tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct
    40621 acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga
    40681 gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct
    40741 aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc
    40801 aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag
    40861 ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga
    40921 ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg
    40981 tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag
    41041 taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt
    41101 tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca
    41161 gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta
    41221 cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac
    41281 atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga
    41341 cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa
    41401 cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt
    41461 tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt
    41521 gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat
    41581 gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt
    41641 ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa
    41701 aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag
    41761 tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa
    41821 tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga
    41881 ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg
    41941 tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac
    42001 gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa
    42061 aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata tatacattgg
    42121 gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag
    42181 tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat
    42241 attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat
    42301 cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat
    42361 actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg
    42421 agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc
    42481 tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa
    42541 aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga
    42601 taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct
    42661 ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata
    42721 attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa
    42781 gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta
    42841 aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat
    42901 ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag
    42961 cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa
    43021 aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct
    43081 gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat
    43141 ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc
    43201 catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt
    43261 caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt
    43321 ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg
    43381 atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta
    43441 agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa
    43501 tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat
    43561 attactcata tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct
    43621 aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca
    43681 aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca
    43741 gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac
    43801 gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta
    43861 gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta
    43921 ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat
    43981 ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag
    44041 aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt
    44101 tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt
    44161 catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg
    44221 gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga
    44281 ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta
    44341 agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat
    44401 ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac
    44461 cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg
    44521 tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg
    44581 aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag
    44641 tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg
    44701 gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg
    44761 gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga
    44821 aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg
    44881 atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag
    44941 atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga
    45001 cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa
    45061 agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct
    45121 ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg
    45181 taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca
    45241 aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta
    45301 caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc
    45361 gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata
    45421 ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt
    45481 ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag
    45541 taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag
    45601 aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc
    45661 gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag
    45721 gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca
    45781 aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg
    45841 atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt
    45901 ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca
    45961 agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc
    46021 aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg
    46081 tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct
    46141 ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact
    46201 gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag
    46261 tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt
    46321 accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct
    46381 tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc
    46441 ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa
    46501 tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga
    46561 agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct
    46621 ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa
    46681 atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact
    46741 ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg
    46801 attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg
    46861 gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc
    46921 tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga
    46981 tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt
    47041 gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga
    47101 ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga
    47161 tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt
    47221 taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt
    47281 tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga
    47341 aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac
    47401 tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt
    47461 taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa
    47521 aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa
    47581 caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac
    47641 aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga
    47701 tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga
    47761 aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg
    47821 agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact
    47881 gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga
    47941 agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt
    48001 tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga
    48061 tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt
    48121 ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt
    48181 gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt
    48241 agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa
    48301 aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata
    48361 tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa
    48421 aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta
    48481 taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt
    48541 cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa
    48601 tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag
    48661 cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata
    48721 ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga
    48781 aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc
    48841 aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat
    48901 aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat
    48961 catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc
    49021 tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact
    49081 tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat
    49141 gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac
    49201 tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact
    49261 tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga
    49321 actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg
    49381 atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct
    49441 ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg
    49501 atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta
    49561 gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc
    49621 tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt
    49681 gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt
    49741 caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat
    49801 gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca
    49861 gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt
    49921 atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg
    49981 atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca
    50041 gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat
    50101 gggcaactga tcattgattt ggtgcaaaaa tatattgcag aaccattcct aggattatct
    50161 caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt
    50221 attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta
    50281 atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta
    50341 gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca
    50401 tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa
    50461 cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac
    50521 gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg
    50581 attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct
    50641 ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca
    50701 ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt
    50761 tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa
    50821 gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg
    50881 aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga
    50941 aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt
    51001 ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa
    51061 agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta
    51121 aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt
    51181 gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac
    51241 aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga
    51301 agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt
    51361 ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca
    51421 gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg
    51481 gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca
    51541 acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag
    51601 gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt
    51661 ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc
    51721 gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag
    51781 ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg
    51841 taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg
    51901 aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac
    51961 gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc
    52021 taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa
    52081 tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc
    52141 agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg
    52201 cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca
    52261 gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc
    52321 gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa
    52381 accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag
    52441 gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt
    52501 atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt
    52561 tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt
    52621 catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa
    52681 tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc
    52741 catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat
    52801 ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag
    52861 aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag
    52921 ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg
    52981 aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat
    53041 aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga
    53101 aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt
    53161 tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt
    53221 tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa
    53281 ggacctccct acattatttc attctacctc tagtttatca tctgttaagg actagtggaa
    53341 acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa
    53401 acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc
    53461 tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtataaataa cccctgtatc
    53521 ctttgcggta caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat
    53581 ttttatataa actatcgaac acagtattga tttttt
//
LOCUS       DAJSMI010000003        52917 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJSMI010000003 DAJSMI010000000
VERSION     DAJSMI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.003"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..1037)
                     /gene="recA"
                     /locus_tag="ON430_000138"
     CDS             complement(<1..1037)
                     /gene="recA"
                     /locus_tag="ON430_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR3218089.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELP"
     gene            complement(1146..2387)
                     /locus_tag="ON430_000139"
     CDS             complement(1146..2387)
                     /locus_tag="ON430_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCR3218090.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2627..3205)
                     /gene="pgsA"
                     /locus_tag="ON430_000140"
     CDS             complement(2627..3205)
                     /gene="pgsA"
                     /locus_tag="ON430_000140"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR3218091.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPCWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3414..4337)
                     /locus_tag="ON430_000141"
     CDS             complement(3414..4337)
                     /locus_tag="ON430_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3218092.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5076..6362)
                     /locus_tag="ON430_000142"
     CDS             complement(5076..6362)
                     /locus_tag="ON430_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR3218093.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6355..7623)
                     /locus_tag="ON430_000143"
     CDS             complement(6355..7623)
                     /locus_tag="ON430_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR3218094.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7875..8336
                     /gene="mscL"
                     /locus_tag="ON430_000144"
     CDS             7875..8336
                     /gene="mscL"
                     /locus_tag="ON430_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR3218095.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8395..8841)
                     /locus_tag="ON430_000145"
     CDS             complement(8395..8841)
                     /locus_tag="ON430_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3218096.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(8934..9179)
                     /locus_tag="ON430_000146"
     CDS             complement(8934..9179)
                     /locus_tag="ON430_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCR3218097.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9294..9872)
                     /locus_tag="ON430_000147"
     CDS             complement(9294..9872)
                     /locus_tag="ON430_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3218098.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(9949..10479)
                     /locus_tag="ON430_000148"
     CDS             complement(9949..10479)
                     /locus_tag="ON430_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCR3218099.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10483..11343)
                     /gene="rlmB"
                     /locus_tag="ON430_000149"
     CDS             complement(10483..11343)
                     /gene="rlmB"
                     /locus_tag="ON430_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR3218100.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11336..11731)
                     /locus_tag="ON430_000150"
     CDS             complement(11336..11731)
                     /locus_tag="ON430_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCR3218101.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11728..13140)
                     /locus_tag="ON430_000151"
     CDS             complement(11728..13140)
                     /locus_tag="ON430_000151"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR3218102.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13137..13673)
                     /gene="cysE"
                     /locus_tag="ON430_000152"
     CDS             complement(13137..13673)
                     /gene="cysE"
                     /locus_tag="ON430_000152"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR3218103.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13759..13998)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(13995..15455)
                     /locus_tag="ON430_000153"
     CDS             complement(13995..15455)
                     /locus_tag="ON430_000153"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR3218104.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15625..16782)
                     /locus_tag="ON430_000154"
     CDS             complement(15625..16782)
                     /locus_tag="ON430_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCR3218105.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16816..18186)
                     /gene="radA"
                     /locus_tag="ON430_000155"
     CDS             complement(16816..18186)
                     /gene="radA"
                     /locus_tag="ON430_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR3218106.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18249..18776)
                     /locus_tag="ON430_000156"
     CDS             complement(18249..18776)
                     /locus_tag="ON430_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR3218107.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            19006..19812
                     /gene="proB"
                     /locus_tag="ON430_000157"
     CDS             19006..19812
                     /gene="proB"
                     /locus_tag="ON430_000157"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR3218108.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            19809..21056
                     /locus_tag="ON430_000158"
     CDS             19809..21056
                     /locus_tag="ON430_000158"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR3218109.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            21132..21476
                     /locus_tag="ON430_000159"
     CDS             21132..21476
                     /locus_tag="ON430_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218110.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(21593..21919)
                     /locus_tag="ON430_000160"
     CDS             complement(21593..21919)
                     /locus_tag="ON430_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218111.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(22292..23493)
                     /locus_tag="ON430_000161"
                     /pseudo
     CDS             complement(22292..23493)
                     /locus_tag="ON430_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            23610..24125
                     /locus_tag="ON430_000162"
     CDS             23610..24125
                     /locus_tag="ON430_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3218112.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(24324..24770)
                     /locus_tag="ON430_000163"
     CDS             complement(24324..24770)
                     /locus_tag="ON430_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR3218113.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(24817..26295)
                     /gene="msr(C)"
                     /locus_tag="ON430_000164"
     CDS             complement(24817..26295)
                     /gene="msr(C)"
                     /locus_tag="ON430_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR3218114.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(26649..27278)
                     /locus_tag="ON430_000165"
     CDS             complement(26649..27278)
                     /locus_tag="ON430_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3218115.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(27262..27726)
                     /locus_tag="ON430_000166"
     CDS             complement(27262..27726)
                     /locus_tag="ON430_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3218116.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(28213..29481)
                     /locus_tag="ON430_000167"
     CDS             complement(28213..29481)
                     /locus_tag="ON430_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3218117.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(29712..30449)
                     /locus_tag="ON430_000168"
     CDS             complement(29712..30449)
                     /locus_tag="ON430_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3218118.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(30516..31085)
                     /locus_tag="ON430_000169"
     CDS             complement(30516..31085)
                     /locus_tag="ON430_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR3218119.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            31213..32085
                     /locus_tag="ON430_000170"
     CDS             31213..32085
                     /locus_tag="ON430_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3218120.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            32427..33299
                     /locus_tag="ON430_000171"
     CDS             32427..33299
                     /locus_tag="ON430_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3218121.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            33296..35254
                     /locus_tag="ON430_000172"
     CDS             33296..35254
                     /locus_tag="ON430_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3218122.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(35505..36356)
                     /locus_tag="ON430_000173"
     CDS             complement(35505..36356)
                     /locus_tag="ON430_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3218123.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            36479..36946
                     /locus_tag="ON430_000174"
     CDS             36479..36946
                     /locus_tag="ON430_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCR3218124.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(37209..37691)
                     /locus_tag="ON430_000175"
     CDS             complement(37209..37691)
                     /locus_tag="ON430_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR3218125.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(37791..38654)
                     /locus_tag="ON430_000176"
     CDS             complement(37791..38654)
                     /locus_tag="ON430_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3218126.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            39252..40100
                     /locus_tag="ON430_000177"
     CDS             39252..40100
                     /locus_tag="ON430_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218127.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(40271..40612)
                     /locus_tag="ON430_000178"
     CDS             complement(40271..40612)
                     /locus_tag="ON430_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HCR3218128.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            40698..41405
                     /locus_tag="ON430_000179"
     CDS             40698..41405
                     /locus_tag="ON430_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR3218129.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            41392..41715
                     /locus_tag="ON430_000180"
     CDS             41392..41715
                     /locus_tag="ON430_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR3218130.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(41804..42841)
                     /locus_tag="ON430_000181"
     CDS             complement(41804..42841)
                     /locus_tag="ON430_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3218131.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(43288..44271)
                     /locus_tag="ON430_000182"
     CDS             complement(43288..44271)
                     /locus_tag="ON430_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCR3218132.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(44687..45457)
                     /locus_tag="ON430_000183"
     CDS             complement(44687..45457)
                     /locus_tag="ON430_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218133.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(45450..46385)
                     /locus_tag="ON430_000184"
     CDS             complement(45450..46385)
                     /locus_tag="ON430_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218134.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(46979..48628)
                     /locus_tag="ON430_000185"
     CDS             complement(46979..48628)
                     /locus_tag="ON430_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR3218135.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(48823..49719)
                     /locus_tag="ON430_000186"
     CDS             complement(48823..49719)
                     /locus_tag="ON430_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3218136.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(49712..50392)
                     /locus_tag="ON430_000187"
     CDS             complement(49712..50392)
                     /locus_tag="ON430_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3218137.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(50393..51175)
                     /locus_tag="ON430_000188"
     CDS             complement(50393..51175)
                     /locus_tag="ON430_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218138.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(51168..52085)
                     /locus_tag="ON430_000189"
     CDS             complement(51168..52085)
                     /locus_tag="ON430_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3218139.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(52088..52372)
                     /locus_tag="ON430_000190"
     CDS             complement(52088..52372)
                     /locus_tag="ON430_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218140.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT        15158 a        11302 c         9477 g        16980 t
ORIGIN      
        1 aaaggcaatt cttcttgtcc tttttcatct tctgggacag cgacttcttc accaatacca
       61 taagctgctc taactagagc agaaacttct gccatcattt caggatgatt agccatatag
      121 attttagcgt tttcccgtcc ttgaccaatc cgatcttctt tataagaata ccaagcaccg
      181 cttttatcaa caatgtcttt ttctactgcc atgtctagaa gttctccttc ttgtgagatc
      241 cctaaaccat acatgacatc tacttcagcg attttgaaag gaggtgctac tttgtttttt
      301 acgactttga tctttgtacg gttaccaaca atatctgtac cttgtttcaa ttgttctgca
      361 cgacgtactt ccaaacggat cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt
      421 tctggattcc caaacatcac gcctactttt tcacggattt ggttgatgaa aatagcgatt
      481 gtttttgttt tgttgatcga accagagagt ttacgcaatg cttgagacat caaacgtgct
      541 tgtaacccga catgtgagtc acccatttcg ccgtcgattt ctgctcgtgg aactaaagca
      601 gcaactgaat cgactactac gatatctaca gccccacttg atactaaagc atcagcgatc
      661 tctagacctt gttctcctgt gtcaggctgt gaaagaagta gttcatcgat attcacacct
      721 aatttttgtg catattgcgg atctaacgca tgctcagcat caatgaaagc ggccgttccg
      781 ccattttttt gtacttctgc aatagcgtgt agtgcaactg ttgttttacc tgaactttca
      841 ggtccatata cttcgatgat acgtccacgg gggtaaccgc cgacacctaa agcgacatct
      901 aacgcaagag agccacttgg aattgtagag atttgttggt cgattttttc gcccaatttc
      961 ataatagagc ctttaccata gctcttttca atttttttca gtgcagcatc taaggctgct
     1021 ttacgatcat ctgccaatac gataatcctc cttatattta caaatcagag caacatttca
     1081 ttatccttat catatctttt ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc
     1141 atttatcatt ttcttaaaag ctgttttctc aaaagatcaa atccttgcat aaccgcatta
     1201 tatctgatag cagcacgatc tctggcaaag tgatattttt ccgaaaccac gccgtcttta
     1261 gatgaaatag cgatccatac tgttcctgca gattgacctt ctaacgtgtc cggtccagcc
     1321 actccggtga aagctacact gtaatctgtt cttgctatct cacgtgcttg aatggccatc
     1381 tgctccacac attctttgct gactgttcca tattctgcta atagctccgg atcaatctcc
     1441 agaaaaccag cttttgtttg gagcgagtag gtaacgaatc cgcctggaaa tacttcagat
     1501 acaccagcaa tgtcgcctaa tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct
     1561 gttacagtct gcttgttttc tttcagtagt tccactacga cttttgctag cgaattatca
     1621 tcgccgtatc cataaaaata ttcccctact cgttcttgga ttttttcttc taatgctaaa
     1681 agagcttgat tccctgcctg aacatcattt gttttcactg tcagtcgtaa tgtcacttca
     1741 tttggtttgg catatggtgc gatcgttgga ttgatctgtg tctcaatcag atcttttagc
     1801 tcagtgacca gtcgcgattc accaatccca taaaagcgca aaactttaga aattagtttt
     1861 tcttcagaag gaaaacgttc ctctagtaaa ggacgtactt gttccacaaa cattggtttc
     1921 aactcattag gcggaccggg aagaaggata tacgcatgtg tatctgtttg ataaaaagtc
     1981 cctaaagcaa gccctgtacg atttggtaga ggaaccccgc cttcgataat ctgggactgt
     2041 tgcagattgt ttttggtcat ttttcgatgg gttgtttcaa aatatgccaa taattttttg
     2101 tatccttccg tattttgtat gagagactta ccgaggtgag ccgcagttgc ttcttttgtc
     2161 aggtcatctt ctgtcggtcc taatccgcca cataagagta tgagttcact tcttgtttca
     2221 gctagagcaa tcaactcttc taaacgctgc tgattatctc ctacaactgt ttgataataa
     2281 acttcgatac ctagatcagc tagctgttct gacaagaaag tggcattcgt attcacgact
     2341 tgtcctagta aaagttctgt tccgacagct attatttctg ctttcattac ttcccgctcc
     2401 cttatatagt atacgctgta tttttatatt tttttattta aaaagagggc aaggagctgt
     2461 gacaaaaatc ttgtcacagc tccttgttcc aaataaacag tgttctcctg ccaaaacctc
     2521 ggtcataagt cacaatatac ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg
     2581 actcagagtt agacggcagg ctcacaacct ctccactaaa cgatacttac attgatcctt
     2641 tgaacacacc gctgtttttg atgaagtaat ccacacctga atagatagta aagatcagac
     2701 aagcataaag catgatttga tctactggaa taccgatcag gctgaatggg atattattga
     2761 taaacagcaa gataatagca accatttgtg tagccgtctt gacttttccc ggccaagctg
     2821 cagccatgac ttctccatgt tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac
     2881 gacagacaat gatcgctact acccaacaag gggcctttcc ttggtcaacc aacatgataa
     2941 aagcagtcat aactagcatt ttatctgcca acgggtccgc aaacttgcca aaattagtga
     3001 caaggcctcg cgcacgggca atttttccat ctaaccagtc tgtgatacta gctaaagcaa
     3061 aaataactgc acctactagc tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc
     3121 cccaatctaa aggtaaacct acaaccaaaa tgaaaatcgg gatcatacaa attctaagta
     3181 cagttaattt atttggtaaa ttcacactat cgactccttt cttccctatt ctacgtgact
     3241 atcagcgaag tttccaccga tattatttac ttttccaaat gaatttattc aaaggagtat
     3301 ttttattgaa attgatcttt ttgaattaac gaagaaggac cgcgacacag atttgtcacg
     3361 atccttcttt tttataccta caagtaaaca gatgcgtaaa ctaatttata cgatcattct
     3421 gcgtattgaa gagtaaggtt cacatttttt ggactctgat tgttttcccc tggattaact
     3481 ggtacggatt ctccattcgc tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc
     3541 gcatttgttg ctgtttccgg taacacggta ctctgtgttt catttgctgc cagtgtccct
     3601 tgataaacat aggcattgtc tactagcaca cctaaccata cacgattgac agcagtaaat
     3661 tcaagggtta caggcttggt agcgtctgtg atatttactg cgatggcact gccagaatct
     3721 tctcctgtag ctattgccat tttcttttct tcacttgatt ttgttgttga ttctactgta
     3781 gaagactctt tggtctgcgt gctttgctct gaggaagagg actcctcgac tgatccttct
     3841 actttaagcg aagaagctgt gtctccgatt attggatcag cttgacgatc ttgccaagtc
     3901 atatagccca cgacaacgac aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga
     3961 cttgtccaaa agcgacttgg atgcgattct tcctgatgca ttgcttttct agaagtactt
     4021 actgtttcag gctgcggacg ttcagggacc gatcctgtca atttgctttt cccatcaaaa
     4081 acatcgacta aatggtctcc atcttctcct acagcttcag cgtattgccg aataaatgcc
     4141 cgcacgtaaa atttaccagg caattggtca aaatcatcat tgtcgattgc ttccaaataa
     4201 cgtttttgaa tttttgtaat ctgctgtaat tcatctaaag atattttttt attcaggcgt
     4261 gcttgccgta atttttctcc gatggtttca aaagccactc gttcatcttc tccagtctgt
     4321 ttgattctcc gaagcatctg ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa
     4381 aagtcatgtc acagctcctt ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc
     4441 acaagtcaca atatccaata aatataagaa gctgtttttt gaacttgttc cttgtgactc
     4501 ggagttaaac ggcaggctca cgacctcttt cttaaacggt gttcaatcag ctgttcctcg
     4561 gtattagctc aaaaaccggc aaaatataag gagatatttt tccgtatgtt tctctaatac
     4621 tcaggtcaaa acgctgattt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct
     4681 gcggtaataa gtccaaccaa gttaaaaaca ttaagaagct gtttttactt ggctgttctt
     4741 attacttgat gcagggcagc tttttcacaa cctcttggta cacggtgttc cagaagtagc
     4801 ttcttcggca ataagccgaa atatcacaaa aatttgagaa gcaattttcg taaattcctt
     4861 cttatttctt gaagctgacc acttctgtca caacctcttt gattacttcg tttgatttta
     4921 ttttaatata agttgattaa aagagagcat gatttgtttt attttctcac taatttttcc
     4981 aacacaacta agaataccat aaaagctgat tttggaggag gttcctacta caagtctaaa
     5041 acgaaattta agacttttcc tcataaaata ctggtttatt ttggtctcat gtaaaactcg
     5101 ctgaaggcct cttcacttat aaatgcttcc ccagctgcca gtacgtcttt catttggatc
     5161 gaatcgatga tttcaggaag atcaaataaa gttcgatcgc caaataaact ttgcgtaaat
     5221 tgattggcaa tatattcgat cgaatttaat gattggaaat attgtccaag cattttcttc
     5281 ttcagcaaat cgagatttgt ttctgacact tccggatcat cagcgaatcc aagaatgatt
     5341 tctttgactt tttctgctgc tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag
     5401 tgaaattcac gatccaagct gaattcaaaa ccaaagctgt catcgataat tccttgattg
     5461 tacatcgcta gatagtttcg agaagtattg cctaaaatca tttggaacaa cagattgatt
     5521 gctgttttat agcgtaacag ttctgttcct tcttgcggca atgtgtctaa tcctttaata
     5581 ccaagtacaa atttatctcg tgtaatggct gcttccaatg cactttgttt gatgatttct
     5641 tttgtgtttt cagggaaata acgtacaatt tcttgctttg gagggaaatt ttttgcttct
     5701 tggttttccc gaataagttt catcaatttc tcaggctcca tcttaccaac tacaaataaa
     5761 accatattgc ttggctgata aaacgtccga tagcacgtat acaagtcttg agctgtgatt
     5821 ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca atgggtgtgt aggatacaag
     5881 ttatttagta tgccaaaaaa catccgccag ttagggtcgt cttcatacat ttggatttcc
     5941 tgaccgatga ttcctttttc cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca
     6001 aaatcgatca gagtcgtcag attcttttct acctgatccg tcgtagaaaa cagatagctg
     6061 gttttcgtaa aacttgtgaa agcattggcg gaagctcctt gtttcccaaa taattgaaag
     6121 acatcaccat cttctttttc aaacaactta tgttctaaaa aatgggcaat tccatctgga
     6181 actttcacct tttctttttc accataggga ataaattcgt tatcgatcga accataattc
     6241 gtactgaaca aaccatatgt cttgtgatag tcatttttag gcaacaaata cacggtcaaa
     6301 ccgtttggca gtacttcatg ataaagtgtt tcattgattt gctcatattc tgttttattc
     6361 atttgcattt cctcctgcta aaaagaagat tgcctgcaat tcgatttgct cagctacttg
     6421 ttgtatctct ttgatcgtta cgctctccat acgtttcaac cattcttcat cagataattt
     6481 tgtttccggc agccagctgt ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag
     6541 agaaagcaga tattggtttc tcagcattgc ttttgtctga gacagttcca actctgttat
     6601 ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga tgaagtactt gatttcggtt
     6661 cttttcatcg ataccggttt ggacactcat gaatcctcgg aacgtatcca cgctactaga
     6721 ggcataataa gctagacttt ctttctctct gacattcatg aaaagtttag agtgaggaaa
     6781 accgccaaat aaaccgttaa agaccattag cgcaaaacgt tctggttcat cgtaatacac
     6841 atttgtctga taagctaggt tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc
     6901 cttcacgata ttcacttgag gttgggtata aaacagatct ggataaactg tttcccgcga
     6961 ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac
     7021 aaaaatgtcg atttgatcat ttgccatcat ttcttgatag taggacgcta gcgaactagc
     7081 agtgatcttg tccaaatctt cgactacgcc caaccctggg atcttttgtt ccgggctgtt
     7141 ttcaaaataa agttcttgca aggcatagga agcaaaagtt tgtttatctt ccttcaaact
     7201 ttccaaatat agacgcagat tgttcttttc caagtcaaac gtcaactgat caaactgatg
     7261 atttttaata tttggataaa ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag
     7321 gtttggatca ttgacatatt caccattcac aaaactgatc cctgcgttta gccaatgtaa
     7381 atttcctttt tttctaactg ataatccaaa gcttgcgcca taaagctctg caagttgcgc
     7441 acttaacttt gtctgatccg gataattcaa actatttgtt tccaacatgc tgctgagaag
     7501 tgaacgtttc gtgatgactt cttgttgcag acgcgcagta aaacggataa aaatccggat
     7561 cgttttgtat tgttttgttg gtaagacatg aagattcacg ccttctgcca attgatacac
     7621 catatttata tgctcctttt cacagttgat tgcccgctta ctcttttaat gggtcaacct
     7681 gcctttttta cttattcctc tgcttgaaat ttataagttc aggcaattcg ccgttttttc
     7741 taaatgatgt tacactttat agtatagcat agatgttttt tatcaaaaga aacagacggc
     7801 taaaagcgta tattgttttt ttatttttta tttttcgata tactaaagac aaataaaaga
     7861 agggagggtc attcatgatc aaagaattca aagagttcat catgcgtgga gatgtcttgg
     7921 atcttgctgt cggcgttgtt attggtagtg cgtttacaag catcgtaaac caagtggtgc
     7981 aaggcttgat cacaccacta ataggttggt ttgtcgcaat gatcacaggt acttctgatt
     8041 tagaaggagc actttcaatt cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg
     8101 gtaacgttat tagtgctatc attacatttc tgatcactgg ttttgtctta ttcttagttg
     8161 tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga agagcaagtg gaagtggaag
     8221 aaactcctac tgcagaagat tatcttagtg acatacgcga tcttcttgcc aaacaagtgg
     8281 cagaagacaa tacacatcca gtagagcaaa caaatctttc cgatacttct aaataataaa
     8341 gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt ctttttttag attcttaact
     8401 tgctacttgt tcctttttca tgtcgatatg tggaatatca tcttccaaat acacatctga
     8461 gattgccttg aatccaaacg actcataaaa agcttgtagg tattcttgtg cgccgatcac
     8521 gattggtttt tctggaaaat ttttctggat catgttaatc gtttcttgaa caatctttct
     8581 tcctaaacca tctttacggt tgtcctgatg aacaaggaca cgtccaaaat ggatactctc
     8641 cgcttcttca tatactcgcg tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt
     8701 atgccaagct tgtccatcaa tgtcatcaac ttcttgataa ggacactgtt gttccacaac
     8761 aaaaactgcg attcgttctt tgacgatcgc gtaaaactct gctgttgaca attcttcaaa
     8821 tttctttacc tgatattcca ctaagcaaac gccccctgct tttttattct tagcataccg
     8881 attcgcacac aaaaaagcaa acgaacctta cgaatctcag gctcgtttgc tttttaaact
     8941 gcttcagtta aaaattcgat ctcaactgtc cgagtaagta catctgaata gctgtaagat
     9001 actcgttcaa aagaattttc ttcaggatcc aaatcaacaa cgaatactga aggataggtt
     9061 tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc ctgtctgagc aaccaacatg
     9121 attgggctgc cgatacgacc ttctaaatct ttcttgattg aagctaaagt tgttggcata
     9181 cagttcacct ctactatcaa ctataccaca tatttttaaa attttcaagt ttaccataaa
     9241 aaaaaaagaa atgcaataaa aataaataag aagctcatta agatttttct aaattattcc
     9301 tcattataaa atttcaagtg tttttttatt ttgagtttta ctctcccgta agcgctcatt
     9361 acttgcttgt gatccattgc taaacattca gccatgtcta caaaatcaac gccttgcata
     9421 taataatgaa aaattttagc ttctattttt gaaaactgga tttcgcaatt ttcgattgct
     9481 tcttttaata aaagatgctg aatcgctaaa ggttcttgca atgtgatctc tgctaaaaag
     9541 tctggtcctt ctgtctctat tttttggtcg attgagactt catctttttg atcacgacgc
     9601 ttcagcgcgt tctgcttcct caacagagaa tagatttgat tgcttataat gcttttataa
     9661 taacttccaa agctaacacc tttgcttttt tcgtaagttc tcaatgcctg atataaagcg
     9721 atccttgctt cttgttccca atcctcagaa acataatttc ttaaataata ggtgtttttc
     9781 aaacgtagta aaagtggaaa atatttttga tacagggcag caaatgcttc ttcgtcacca
     9841 ttacgtgcag cgcgcaataa ctcctcagac ataaaataat tcctccataa attcgtttat
     9901 ggaggaatct tatcatcaat gattcatcga actgattaga acgatatgtt atttattttt
     9961 tctcattaaa tcgtccaatt tttgggacaa tttttttaac tgctcttcat tccatggaga
    10021 attacgtcgg aaatcaataa aatgcatatc tttattttct tgggctattg ttttttccgt
    10081 tttttttatc gtcttatata attcataagc agaagttcgt aatgctcctt gggaaaagat
    10141 catccactgt tctgctaaat cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag
    10201 ctgaccagcg attctctcga tataactgtc agccgtttcg tcttctttcg taaaaacaac
    10261 atccaactgg tatttcttat aattttgttg gatgcccgga actagttggg catcgaagac
    10321 aacgatgact tccattcctt catatttcgc ataatttgat aaacgatgta aaagagcttc
    10381 tcttgcatcc gccattttgt cctgcttttt aagctggaca agttccggcc acgaaccaat
    10441 catgttgtag ccgtctacaa tcaacagctg ttttttcatg gtctactccc cctgacgttg
    10501 ggaaaaagcc tgatacataa gcaaaccagc agcaacactg gcatttaagc tctggacgtg
    10561 cccggtcatc gggatcgtca gcaattcatc catttctttt ttcaatcctg gactcattcc
    10621 tttgccttca ttaccaatga ctaaagcaat tgatcctttg gcattccatt tttggaagtt
    10681 tgtgccggtc atatctgtgc caaataccca aaatcctgtt tctttgagct gttgcacagc
    10741 ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg actgccccag tggaagtctt
    10801 tgttacgata ggtgtgattc caactgctcg gtgttttggt atgatcacac catcgacgcc
    10861 acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa
    10921 aatcaaaaag aaggggtctt cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga
    10981 taaatattca taaggtgtaa tcgcaagaac gactccttga tgcacgccgt gatcgctcaa
    11041 agtttctaat ttttgtttag gaacccattt gactggcaca gcttgttcac gtgcgatttc
    11101 tttcagttct tctacttttt ctcctcgtga atcttcttga agaaagagtt tattccctcg
    11161 cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag acgaaattat cttctaatgc
    11221 ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt tgctttttat ttgaatatct
    11281 attttgtttt ttgtcttcaa atccacgttt cgattgatgt ttgtttgttc tttctttacc
    11341 catttttttc accaactttc tgaatacacc agcggatcaa ttcttccatc cgctcggttt
    11401 gttttgtcaa atgcagatag cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg
    11461 taacatcagc attttttgcg ctcgtatggc ttttagcatt tcttcctctg cgataataaa
    11521 gttcttcttc ttctgtcaaa agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg
    11581 cattcgcaga aacataattc gttgccgcac gatgcagctt attgggtttt gtttctcctt
    11641 gatcaatcag gtggtcacga acatagattt cgtaaatcgc atctccaaca taagccaagg
    11701 ctaaaccgtt caattgtgtg taatctctca tgcttctctt ctccatcggg ttccttgcgg
    11761 tgtatcttct aataagatcc ctttttcttt caacaaatca cgaatttcgt cgcttcggac
    11821 aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc aattgttcaa tctcttcatc
    11881 taataactct tctgctgtaa ataaaatacc gaagatcgaa agccattgcg agaataaagc
    11941 ttctccttgt gccaagacag cggcagaaac tttcggctgg tctagataag tgttcagcca
    12001 cttagcaagc tcatagacca ctgtgatccc atttgctgca ttaaaatcat cgtccatttc
    12061 ttcgatgaat cgctgttcta aattttctaa caaagcgatt ttatccgcat catctgccaa
    12121 attctctgag gcagtttctt ggcggaattt caaattgtca aacgtattct tcagtcgttg
    12181 taaattggtg gctgcttctt tcatcgtcgt ttcactgtag cggatcggac gacggtattg
    12241 tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg tttttgatca tttcatgtac
    12301 agtgatgaaa ttccctagag atttactcat tttttcatcg tcttcgccaa tcgttacata
    12361 gccattgtgc atccagtaat tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat
    12421 ttcattttca tgatgaggaa attctaaatc ttgcccgcca ccatggatat cgatcgtttc
    12481 tcctaaatgt tttgttgcca taacggaaca ttcaatatgc caaccgggac gtcctttgcc
    12541 ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt ttccataaag cgaaatctaa
    12601 tgggtcttct ttttgctgct gttcttcccc tgtacgctga cttgctccga cttccagttc
    12661 atcgatggat tggtcactta atttcccata attttcaaat ttacgggtac ggtaatacac
    12721 atcacctgaa gattcataag catagccttt ttcaaccaac acttgaataa atgctaaaat
    12781 atctggcata tgatccatga ctcttggatg cagtgtagca ggctcgacat tcaacgcttt
    12841 cgtatcttct tcaaatgctc ggatgaaacg atcagcaact tctggtgctg taatacctaa
    12901 ttcgtttgct gtacggatga ttttatcgtc tacatccgtg aaattcgata cgaaatcgac
    12961 tttatagcct cgatattcaa aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc
    13021 atttccgata tggatataat tatacacagt cggtccgcag acatacatac cgacatgtcc
    13081 cttttgtata gggatgaatt cttctttttt tctggtcaat gtattgtaga tttttatcat
    13141 tctgatctcc tcactttttc tcctttgatt cgaacgactt ttgctggtac tcctaccgct
    13201 gtagcgtcgt ctggaacatc tttcaacaca acggcacctg caccgatttt ggcgtttttt
    13261 ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca
    13321 gttgggtgtc ttttacccgt ttcttttcct gtgccgccta atgttacgcc atggaataaa
    13381 atcacatcgt cctcgatttc tgcggtttcg ccaatgacga ttcccattcc atggtcgatg
    13441 aacacgccag ttccaatcgt tgctcctgga tggatctcaa caccagttag gaaacgccag
    13501 aattgagcat tgatcttcgc caataaaaag agccggtgct gatataaaaa atgagagaaa
    13561 cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt
    13621 gcagccggat cgtttctctt tgctgcatca atcgctcggg taaaccaact catgactttc
    13681 cactcctttc gtaagaattt tatgtaggaa atcatcttct tataaagaaa agaagcgcct
    13741 tttaaataag tttccttatc aaaagacgct ttccgcgtgg ttccactttt cttcataatc
    13801 aatgattatc tctgacagct aacgcctgtt atacgttctt ggctactttg tttcctataa
    13861 aacagttttt cctgttttct gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc
    13921 attcattgat accttgcacc acccggtatc tctcttgtaa tgatcagctg cttacttttt
    13981 tctgttcatc gtgtttagtt cagtacttga tttaaatgtg ctaatgattt ttcacgacca
    14041 agcaattcga tcgtttcagg caattctgga ccatgcattt gtcctgatac agctacacgg
    14101 attggcatga aaagattttt tccttttacg ccagtttctt tttgaacagc tttgatggca
    14161 gctttgacag ttggagcatc tactacttcc atttgttcta gctgtgcttt gaatgcgtgg
    14221 agaacagtag gaactgtttc accagcaagt acttcttttg ctgcttcatt caatacagga
    14281 tgttcattga aaaatagcgt agacagatca acgatttctg cagcatagct catttgaggt
    14341 tgatacaggc taacgatctt cttcacccat tcgattgtct tttcatctgg gttttctgct
    14401 acacgtccat cagcgattag ataaggcaga cacatgtcag taaatgcatc tagatccatt
    14461 gctttgatgt aatggttgtt tacccattgc agttttttcg catcgaaagc tgcgggtgat
    14521 ttacttaggc gttttggatc gaacattttg atcaattctt ggtgttcgaa gatttcgtct
    14581 tcgcccacag gtgaccagcc caataatgca ataaagttga acattgcatc tggtagatag
    14641 cctaattcgc gatattgttc gataaattgc aagattgatt cgtcacgttt gcttagtttt
    14701 ttacctgttt cactgttgat gatcaatgtc atatgtccga aggttggtgg tgtccagcca
    14761 aatgcttcat agatcatcag ttgttttggt gtattggcaa tatggtcatc tccacgtaaa
    14821 acgtgagtga ttttcatcaa atgatcatcg accgcaacag caaaattata agttggcata
    14881 ccatcgcgtt tttgtatgac aaagtcgcca ccgatattat cggattcgaa cgaaatagcg
    14941 cctttcacca tatctgtaaa tgcgtattct gtattgcgag gcacacggaa acgtacaaca
    15001 gattctagac cttgtgcttc tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt
    15061 cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt cccgttctgc ttctaattct
    15121 tcttctgtgc aatagcattt gtatgctcga ttgctagcaa gcagctgatc aatcagcggt
    15181 tgatagattt ctttacgttc agattgacgg taagggccgt attctccagg attttcagga
    15241 gattcatccc aattcattcc caaccaagca aggttttcca actggctttt ttcgccatct
    15301 tcgatattac gtttttggtc agtatcctca atacgaataa taaagtctcc atcattgtgg
    15361 cgggcaaaca aataattgaa taatgcagtt cttgcatttc caatatgcag gtgcccagtt
    15421 ggacttggtg catagcgtac gcgtactttc gtcattttct tcctcttctt tcaaactaga
    15481 ctttgactaa cagcaaaaat tccgtcagtt acggcttctc aaatatcatt tatttaaagc
    15541 cactcagcac aaattttacc ttttctaagg taaatagtaa agccaactgg ttgaatttcc
    15601 ttaccagttg gctgaatttc ttttttattt tgattgttta gtctttttct ctgtctcttt
    15661 tttattgtcg attcctcgtc ctgcatgtgc aggtttagca aagatcatgc gtccagcagc
    15721 tgtttgaagg gcacttgtta caacgacttc gatacgttca ttcatgtaat gctgtccatc
    15781 ttctacgaca accatcgtgc cgtcatcaag gtaggctact ccttgttggc gttctgtccc
    15841 ggcttttact actagaacat tcatcgtttc accagggatg acgactggtt ttacggcatt
    15901 tgccaacgca ttgatattaa gaacaggtac attttggaat tcagagactt tattcaagtt
    15961 atagtcattg gttacaatga caccatctaa aagcttagcc aatttgatca atttgctgtc
    16021 tacttcactg atatcttcaa agtctccatc gtacatttcg acagatatac cctcttcttt
    16081 ttgcaaggca tttaggatat ctaatccgcg acgaccacgc acacgtttca aactatcccc
    16141 tgagtccgcg atgtattgta gttcgtaaag aacaaaatta gggatcatca aggttccttc
    16201 taagaatcca gtctttgtaa tatcataaat ccgaccgtca atgatcacgc ttgtatccaa
    16261 gattttatat ttatggaaat tttctcctga ttttcgttct aacagttgac tctcaacttg
    16321 actagctgtt tcttgctcga tttttttgct tcgtgaacca aaaactttct tccattcttc
    16381 gattcgtgtc gttcccattc tcaatcccag atagcctaag atgatcatca gtaaaatcgg
    16441 caatacacta ttgacgaatg gaatttccat attgtaaaga ggggtcgaga tcacaacacc
    16501 taaagtcaat ccgatgatcg caccgatcgc accgaagaga agataagtca agctgacctc
    16561 acttagtttt tgttctactt gtttgataat agctgagata tgtttggcca acagcaaaga
    16621 taataaaaag aaaataagtg cgcctaacag gctgttggta aatgtattat tcagccatcc
    16681 gtttgactgt tgaccgattg cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc
    16741 gccgacaatg atcatcagca aagtaatgac acgtttttgc atcgtttttc ctccgtttcg
    16801 acaatcattt attagttagc ggaacacacg cttcaacgtt tcagccaaag ttgctactcc
    16861 aactatctcg attccttttg gcgcttccca gttacctaag ttgtttttag gtaggtatac
    16921 ttttgtgaag ccgagttttt ggacttcttt gactcgttgt tcaatatgac tgacacgtct
    16981 gatttctcca gtcagtccaa tttcaccgat gaaacattcc gttggctgtg ttcctttttc
    17041 tttatagctt gaagcaatac tgacagcgat agctaaatca atcgctggtt catttaattt
    17101 taccccacct gcagctttta agtaagcatc ttgattctga agaagcaacc cagctctttt
    17161 ttccagcact gccatgatca gagatacccg attgaaatca agacctgttg ttgttcgttt
    17221 ggcatttccg aacatagtag gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt
    17281 cccttccatt gccacaacga tagcagagcc agttgcaccg tctaggcgtt cttctaagaa
    17341 aatctgtgaa ggattagcga cttcttccaa tccatgctca tgcatttcaa aaatcccaat
    17401 ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa atccgaaaac tatgatgttg
    17461 ttcaccttca aaatataaca ccgtatccac catatgttct aacattcttg ggccggcgat
    17521 cgagccttct ttggtaacat gaccgacgat aaaaatggct atcccatttg ttttagcgat
    17581 tttgagtagt tctgcagtgg tttctcttac ttggctgaca cttcctgcca cacttgtgat
    17641 gtctggctgc gtcatcgttt ggatggaatc aatgataaca taatcagggg agatatgttc
    17701 gatggcacga ctgatttcgt tcatatctgt ttcagcgtat agataaaatt ctgtgttgat
    17761 acttccgagg cgttccgcgc gtaattttat ctgctctgcg ctttcttcgc cggatacata
    17821 taagactttt ccgcctatgg ctgctaattg ctgagaaact tgtaacagca aagtggattt
    17881 cccgatacct ggatcgccac caatcaagac catagaccct ggcaccacac ctccgcctaa
    17941 cacacggttc agttcttcta attttgtttt gatccgaggt tcttttttag ggacgacgtc
    18001 tgcgattttt gttggttttg ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt
    18061 tgtatcttgt tcgatttctt cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc
    18121 caagaactta ggcgacacat acccgcaatt ttggcaaaca aattgtactt ttgatttttt
    18181 tgccattgta ccgcctcctt ttatcctaat catgagacat taaatcaagc ttaagtatct
    18241 ttaacgtttt atttactgga agaaccaaat ccgccttgac gttgagcagt cgtttgatct
    18301 tgatctgctt ttaaaaacgg caaaaagatc ccttggccga tacgctcgcc ttttttgatc
    18361 actacatcct tgaaaccaaa gttggtgaat tggaacatga tatggccttc atttgcctca
    18421 ttattgtaat aatcactgtc aatcacacct actccgtttg tcaacactaa aaaccgcttc
    18481 aacggattgc ttgaacggtt agcaagctgc aaaaattcat cttctcccat gtaagctttg
    18541 attccagtag gcacgagggt tggtttcaat tgtttttcat tttctgatac atcttttttc
    18601 aatccatctg cgactgtttc caaccccttt ttccagcctg ctttccagat acttggaatc
    18661 acgacctctt ctgctgcttc aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt
    18721 agattgatat tttgatcttt atatttagaa ataacttcaa atcctctttt tctcaccggt
    18781 tttcctccct aaataaacta ctcctattct aacatatccg aggctgtaaa agactttctt
    18841 tacaaattct tcaagaaatt ctgctatctt aaagtaaacc attaattcgt tctttttcca
    18901 aacgactgca agttgtacaa aaaaactatt ttttacatga ctttactcgt caaaacaagt
    18961 agaatagaac gaaattaatg agtggatatt tgattgaggt gtcacatgcg taaagaaatt
    19021 aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta cattgattta tcccaatgga
    19081 aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc tttctgatct tagaaatcgg
    19141 ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg ttggtatgca taaattacat
    19201 ttgtcagaac gtccgacttt gattcctgaa cagcaagctg cggcggctgt tggtcaagcc
    19261 gaactgatga atatctataa tcaaagattc agtgtatata atcaacagac ggcacagatc
    19321 ctcgtgacaa gagacgtggt agattatcct gaaagtcgta aaaatgtgag gaatacattg
    19381 gaacagcttt tgattatgga cacgatccct atcgtgaacg aaaacgatac tgtagcagtg
    19441 gatgaattag atcatctgac aaaatttggc gataatgatc agctgtcagc catcatggca
    19501 aaacttgtcg cggcagacct tttgatcgtc ctttctgata tcgacgggtt ttattcagat
    19561 aatccgacta caaatcccga ggctcagatg tatcgctata tcgataaaat cgatgaaaag
    19621 attctggcac aagcaggtgg tgctggtagc acatttggaa caggcggtat gcagagtaaa
    19681 ttaaaagccg cttctaggat atttgaagca aacagcgcaa tgattttagc aaatggaaaa
    19741 aatccggcaa ttatttttga tattcttgca ggaaaagaag taggtacttt atttaaggag
    19801 gacttgaaat gactgacttg atgcaattag gacaacaggc aaaagatagc gcgcaacaac
    19861 tagcacttat gcctacaaac cgcaaaaatg agctacttgt tcaaatggca caaaccatca
    19921 aaaagaatca acaagcgatc attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa
    19981 acaatatcag tgaaacgatg caagaccgct tgcagctgac acctgagcga atcaatgcca
    20041 tggctacaga gatcgaaaaa attgcttcgt tagacgatcc tattggaaaa gtagatgaga
    20101 tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgaccg
    20161 gcatcattta cgaatctcgt ccaaatgtca cgacagatgc agctagtcta gcattcaaat
    20221 cagggaatgc cgttatcctt cgcggtggca aagaagcatt cttctcgaat caattattag
    20281 tccaattatt gcagcaagta ctggtcagtg ctggcgaatc tccttatgcc atccagtttg
    20341 tcgatgatac ttctcatgaa actgcaggac agttgatgcg attgacagaa tacttagatg
    20401 tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt caaagaacag gcaacggtac
    20461 ctatcattga aacaggtaca ggaaataatc acgtatatat cgataaagaa gcacagttat
    20521 ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg
    20581 cagaaacatt gctgatccat tctgagatcg cacctttttt cttgccagct attgaaaaag
    20641 aactggtaga acacggggtc tctttacgtg cggatgctcg tgcgttagaa tatctagaga
    20701 cggctgcttt agcagaagac acagattggg atactgaata tctagattat atccttgctg
    20761 tcaaagtggt cgattctttg gatgaagcaa ttgcacatat caatcggcat aatactaaac
    20821 attcagaaac gatcgtgaca gatagttatg cggctagtca acgtttctta aatgaagtgg
    20881 acgcggcggc tgtctatgtc aatgcctcca ctcgttttac agacggatcg gtatttggtt
    20941 ttggtgcaga gattggtatc tctactcaaa agttgcatgc ccgtggtcca atggggttaa
    21001 atgaattaac ttccacaaaa tatatcattt atggcgatgg acaaatccgc gaataatgtt
    21061 tcgtgtgaaa cattattccc gccttttgcc ttttttctgt aaaggttcta tccaactaga
    21121 aaggtgtcaa tatgtttgaa attttaatga ttttttttat ttaccttatt tctctgaata
    21181 tcgctgcttt tctgggagtc ggtattttat cccttttctt tcaattcaaa aaacgaagca
    21241 tagggagtca acgggaaaaa tggtcgcagt attttgacaa aatcgggcaa aaagggctgg
    21301 ttacacgctt gcacattagt tacatggttg cattatgctt attagcgaca atcaattact
    21361 attctttttt cgatcattca atcgcttata cgattacgct actgattgct gggatttttc
    21421 acttatctta taaatatcag ctgaataaaa accacttgaa tcggactttc cgctaaaaaa
    21481 tatccgtacg ataagtagag tgccttgaag atcggcaaaa aactgccgga ctttttagag
    21541 gctgtgacaa agattttgtc acagcctctt tttccatata aacggcgttc gcctaccagc
    21601 tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat
    21661 cataataacg aagcagctca tccatggaca agttcatcgt tttttctttt tcttcttccg
    21721 ctgattcagc cagtagctgt tcccgataca cctgatacat accagctatt ttttgctgca
    21781 tcgcttttct agtttcacct tccgcattga ttattggcaa ttctttgata tagccacgat
    21841 aaatcaaccg atcatctgaa aattcaatgg gtaattcaac gagtgtcaac ccgacttcct
    21901 gaaaatattc ttccagcatc tattatcccc ccattctttt tcttaaatga attttagctt
    21961 acctactgaa aattttccag tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc
    22021 gtgtcacatc ccctttttcc gaataaatgg tgttcttctg ccaactcctc aatcataagt
    22081 cacaatattc aacaaacatg agaaactgtt ttttgaaatt gttccttgtg attcagagtt
    22141 aaacgacagt ctcacaacct cttggtaaac ggcgttcaaa agcttgcatc tgcggtaata
    22201 agcccaacca aacgaaaaat atgagaagct atttttgttt ggctgttctt atgacttgat
    22261 gcaggacagc tttttcacaa cctctgattt cttattcttc ttcatttgta gcaaaataat
    22321 tacggtgata aatgatacaa catacactga taagaacaat ggcacctgca aaataaaacg
    22381 catagctttc accaatatgt tcaccaatca cacctgcaat aaatggtgct aaagctccac
    22441 cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt tcgttgcaga cctggtattt
    22501 ccatagcaat cgtcgtcaac agtgtattga taattccttg gaaaaatcct gctaacacaa
    22561 tgctgatcgg tacaaggact ggcatagaag ttccagctcc taataacagc aaacaaataa
    22621 tgaataagac aaatgctgct aaaattgtcc gataagtcgt aaacttccgc tcaagagccg
    22681 gtgcaacgaa aatcgaagca atagccagaa gcacacccca gccaaaaaat acaaaaccga
    22741 cttctaattc agaatagcca atcaataaaa atggcgcata cgcaagtaaa gtaaagaatc
    22801 cgaaattata taacaatgcg attagtccaa ctgaacgaag tttatgattg cttaacgctt
    22861 tgacaccttc ccaaaggcct acttttctct ttggtttctc agtttcaggt aaaagtaccg
    22921 ctagggctat aaaaccgcaa aacatcaaaa atgctacccc aaaaaatgga taacgccatg
    22981 aaccactgcc caagacccca ccgactagag ggccaacagc cattccgcag ccaagtgctg
    23041 cttcatacat catgattgct ttttctgtgt taccaactaa aatactgacg atcgttgcta
    23101 aagcagtaga gacaaataaa gcattcccga caccccagcc ggcacgtaac ccaatcagca
    23161 ttccgatatt cccagataat ccgccaagac cagcgaatac aacgatgata aacaatcctg
    23221 tcatcaatgt tttttcaaac cgatccgact agataagaaa cctgtaaata gcatcaccac
    23281 accagtgact aacatgtaac tagtaaataa cagggtcgtt tcagctggcg tagcgtttaa
    23341 ctgtgcagca atcgttttta agattgggtc tactagaccc actcccataa aagcgatcat
    23401 acaggtagct gctacaacta gcgctgaact tttccccttt gtttcttcat ttgtttgttc
    23461 ttgtatatct atttgattct ccaatatttc catccctttc tctctattag catcatacat
    23521 gatttatttt acatgtaaat atgcacatgt aaaacgtgga aactttactg tgatataata
    23581 aagaaaataa gacgagaggt aggattttta tgaatacttt agcctatata ttattatgcg
    23641 tgcttttatc caaaccacgt tctgggtatg aattgaagca gctgatcaca attttttggg
    23701 aagcccatca cagtcagatc tatacaacgc ttgccaagtt agaccagcag ggttatgtga
    23761 ccgttttgga caaagacgag cactctcaaa aaaagatcta ccacttgact gatgaaggac
    23821 gtgctcttgt agaggaatgg atcaaagagg aaactcccgc tcctacgcaa aaagatgaat
    23881 ttctagcaaa aatctatgct ttttcgacgc tcgataaaac aacagctact ggactgctat
    23941 tcactagaga acaacagctg aacaaaatca tgcaaaaaaa tcaaaaaaaa ttgaatgacc
    24001 tctcgctaga caagaaacaa gaatttggac gttatgttgt gatccagcgc agaattatgc
    24061 tttgtcagca ggaactccaa tggtgtcagc aagtaagaga acaaatgcag cagttttttg
    24121 agtaagggat aaacagaacc tttctagaaa tatctattga agagcaaatt atcttcaata
    24181 aaagagcaat acagttggaa aagaaaaagc gatccgaacg acaggccaaa acctatcgtt
    24241 cggatcacta tttttctaga actgcattca agagactatg acaaaaagca tgccaaggcc
    24301 ttttttcaaa taacagtgtt ccatcattca ttactttgaa ttgcttttag cacacttctt
    24361 gaaagcgggc caacgatgat caactgcaac ggtaaagcgg caatcacatt cattttccaa
    24421 gcattcatat aagcagagaa taattgatca ggtattcctt gttccatgat cacaccgaat
    24481 aaagacatac atgtcaccat tcctaaaacc atcaagcatg aaatagttaa gatcatttgg
    24541 accttgcttt ctggatttac tggtagttta aaggcaattt ttttcgccag cacaccgatg
    24601 atgaagacat ccaaaatgaa agccaccacg aatccaggca ctagaccaat caacaaactt
    24661 gataaatgaa gatcttgatg cagccataaa ttatacgcgc tcatccccaa gaccatcaaa
    24721 aaacacatca ttgttgtaaa aattattcct tcttttttat ttgtaggcaa tctgtaccac
    24781 tcctttaaat ttgtcacgaa aatcatcgta gcacatttaa aaatttctcg taagtacttt
    24841 ttttattttc tgccctttta tttcataaac ttcatcagcc gtacgctcga caaagtaaga
    24901 atcatgagaa gtaaacaaaa tcgttcccgg atatatttgc attagcttct ctaaagcttc
    24961 gatcgttgcc atatcaataa aattagtcgg ttcatctaac agcaacacat tacttggctt
    25021 agtaaaaagc aaagcaatcg tcagacgggt cgcttctccc ccacttaatg tacataacgg
    25081 tttcgtcaaa gtttcctgag caaaccctaa gtgattcagc aaactacgga caagcgattc
    25141 tgaaaactct gtttgtctca tgacaaaacg aatgatggat tcttcagatg tcatttgata
    25201 acccttttgc tgatatactt gaaagcttac tttcggagag agaaggattc cttctccttg
    25261 tttgcggata tgttctaata aggttgtctt acctgaacca ttttcaccga caatcgcgat
    25321 ttttttacct aaagaaaact gaaaatcacc atttaccagt aatgttctcc cacttctttc
    25381 caattggaca ttttgtccca tgatgggata gcggctgtgg agttcaagag attttggtac
    25441 tggaaaacgg atttgtttca tctgctgcgg cttggttgtt tctcctattt tttggagtct
    25501 tctctctaat gttttcgcat tcttttggat ggctttttgt acactgtctt tttgtttaga
    25561 ggaagacaac cgatccggtc tgatttgttg ctttttcttt ttactggaca ctttttccaa
    25621 cttttccgct tgttttttct tttcttggat cgatttttcc aatcgttttt tctctttcat
    25681 gatcgactct gcttctcgta gctgagtctt tttctccaat tccttttgag tgagataggc
    25741 actataattc cccgaaaact ctcggatttc tccgtctttt acttcccaga ttttcgatgc
    25801 tagctcatct agaaaatagc gatcatgact aacaaaaacc agcgtaccat aataataacg
    25861 aagctcttca atcagtcgat caatactttt tctatctaga tgggttgttg gctcgtctag
    25921 aagcatccct tgttcataag aagaaagaag acgtatcaac tcgatctttc gttcttcccc
    25981 tccgctccat ttccgctctg aagtttcttc cagtcttaac tggaaatgac tggctaattc
    26041 caaatttagg tcctttgctt cagcgagata ggttaattga ggcaggtagt tgaaggtgat
    26101 ctctgtttgg attttcccat gatcgggaaa aagttcaccg gctattagtt tgagcaaagt
    26161 cgatttaccg acaccatttt ctcctatgat ccctatacga tcttgctgat agacacgaag
    26221 agaatcaata gatagttcta gctggttgcc aaagctgact tgtagatttg ttatatttac
    26281 tgctaaattt tccatatgtt ttacactcct atttcatgta aaacaacaat tatcgatact
    26341 aaaaaagagc agaaaaatct gtttgcaaaa aaactgcatc acatcttttc ctactctgaa
    26401 cgaaataact atgactaaat caccaaatct gtcatagctt tcacctattc tactgtcgtt
    26461 cgaaaaaatg gacatactcc atccatgaaa ttgttgttta tacatgtctt ttgaatttat
    26521 ttagttgtta ttcaaaagtt cgaaacgtaa tttcatcgat gcagtcattt ttccgacctc
    26581 cttttttatt aggttaccac acacgattca aaaaaagcaa tagctactga atagtaactg
    26641 aaaacctttt attcgaacgt agtgatcttt tctggtgcta aacgaacaga ctcgtaacct
    26701 tcttctaccg cttttcctat tgataaaata ataactggaa cataacggtc tttatccaaa
    26761 ccaaatgcct ctgccaactg atctgcttcg aatccaccaa ttggatttgt ttcatagcca
    26821 tgatcacgcg caactaacat gaattgcata gccgctaagc tagaatcgac tttgactaca
    26881 tcgttcattt cttctcttga aaaactcttg taataaggaa tgatcgcagc taattgttga
    26941 tctctgactt cttttggcat cttccctgat tcgtaagctt ggttatagat ttcttcgcca
    27001 tattcgtaac attgcatatc gccaaaaatc atgaccattg cagaagaagt atcattttgt
    27061 ctcgtattga aacggatcaa aggacgcaag atttcttttg cttcctcgct ttcagcgacc
    27121 acaaagcgcc atggttgcat attgacagaa gaaggtgcag tcgtcgcttc tttgatcatt
    27181 tccagcattt cttcgtgtgg gattttgtat gtttcatcat atacgcgaac ggatcttcta
    27241 ccagtcataa ttgttgaaaa atcatttttt tgttccatta ttattaacct ctttcgtttc
    27301 tgttgttata ttgcaaagtt gggtattcaa tttatctaaa agtgttagaa attgtttcat
    27361 ttctacatcg ctgaaagccg gggatagcac ggaacctgta tctttatggt ccatttcaca
    27421 atgctcgatt ttcttctggg ctttttctgt tagcgctaca tacacttccc ggttgttctg
    27481 aggatttctc tgacgcgaaa catagccatt tgcttccaat accttcagat gacgagtaac
    27541 tgctccttga tcaatctgca acttttcttg caaactgctt tgtagcaatg gattttcttc
    27601 tttcagtctc atcatgatct cgtatctagt aagacttata ccgatatttt cttcaaagat
    27661 ctggacgatt ttttgatcgg ctaatttgat ttgatataaa aggtgactaa tctcttctaa
    27721 agacataatt tcctcctttc taataattga ctcgtcaacg attgacttgt caataataga
    27781 ataaaaaaag aaaaaagtca aagaatcagc tgtgtaataa aaaaaacgct ctcaatagaa
    27841 aagtatagag aaaagaaatt ttttgtagaa tgaacggaaa aaatgataaa gaaagcacaa
    27901 cgctaaaacc aagattattg attcttatca aactatagaa gatagctctt ctgcagtaga
    27961 aaattttaca ccgccaaaaa tttccgaaat cgccttttgc aagaatcccg tcgaatatta
    28021 gaaattggat cagtatgctt aaacagtaat aaaaaagtta tgacaagatg ttgtcacaac
    28081 cttttttgag taaacggtgt tcaaaaactt gcatctgcgg taataagttc aaccaaacaa
    28141 aaaacatgaa aatctgtttt tgtttggctg ttcttactac ttgatgcagg gcagcttttt
    28201 cactacctct ttttaataac cataaaatcc tattccttca tatctccagc ccatcggtgt
    28261 tagccaatct ctttccttag cactcaaggt atagtgatgg gtaccagttt tttcatttgg
    28321 attaaatagt cgataaattg gcttggaacc accagaataa aaacttagac cttcataacg
    28381 ccaaccgatt tttgttaaag agttgatctc agattgtgcc attgtgtaat gatggtctcc
    28441 actgtaagga ttgtataaac ggtagacagg ttgtccgctg tttggtgctt gccaagcaat
    28501 cccttcatat tcccagccta cagttggcag ataatttttt tctccggcat tcaatgtata
    28561 taaatgttct cctgtatgac ggttatacag acgatacatc ggagaagttg aagttggtgc
    28621 tgtatcatac tgtgtcagat tataggtttc gattacattg ttcaatgtag aaccataatt
    28681 aggcgcagtg gcataacgac ctgttagcca agccgtagca tctttgtaag agttcgtatt
    28741 gcttttccat gcaccggaat agtagtaaac gcctggctga aaagaagtcg tcttcaatac
    28801 cgttacgtta tcttgcaatg attctttgta cgaaggatat tttctaaaag cggcattcac
    28861 cgtaatccat tgcccattca catattccga agtaggcatc gtaacagatt ggccattata
    28921 gcttccttta atcccaaata gattgtaatt aggggcagaa gccaaagtac tgttccccca
    28981 gccactttca acgatggctt gagcaatcat gactgatgca tataagtcat tggcacttgc
    29041 caaactttgt gcagttgagg caatactatc aatgaacgct tgttgagaat tagcagatga
    29101 gcgagcataa aaagcatagc cagtatagtt ttcgaattca gacaaatctg cttgatgggt
    29161 atgtgtcaat gtttgcccat ctgtcaattg gttcgtgatt tcttctgcat tctctggatt
    29221 gttctgagct tctgctgatg tactgtcttc ggtttgtgtt tgttctgttg tttcagttgt
    29281 tggttcttcg gttgaaattg tattttctga gacagtagct gactgcaaag tactctctgt
    29341 atcgttttca attcctgttt ctgaagaact cgatgtctga gttgcgacag tttctgtcga
    29401 ttcttcagct agcactgttg tgttggcaac tgctagattt cctaaaagaa taacgctaaa
    29461 taataaattc tttgctttca tcttttttct cctttgaatt atgaaacttt tcctaaataa
    29521 cagtgtaact aaaaaagtaa aagcaagtca atgtccatat ttaaataaca tttgtttctt
    29581 tttgataaat ttgctttttc tacataaaaa gacgtaaatg aagaatattc ttctccattc
    29641 acgccttaaa ttgatggtcg tttgaataac tatttaacaa gtaattctct ttggatatta
    29701 tccgttatgg attatacttc ctgtgtcgtc ggacggataa tcatgttact gacagacatc
    29761 cggtctggcg tttctatcgc aaatgctaca gcagaagcta tatcttcgga ggtcaatccc
    29821 cattcttttt gcgcttcatg aagttcaaga gcagcctttt tgtcgctgat ggtttgatat
    29881 aattcagttt gaacagctcc tggagagatg atcgtagatt tgatgttatt ttgccgttgc
    29941 tcttgacgta atccttccat gattgcacgg acagcaaatt ttgttccgca ataaacggcg
    30001 gaatcaggat aaacaacatg tcccgccaca gaatcagttg aaatgatatg cccagatttt
    30061 tgtttttcca tgattggaag tacggcggaa attccattta acacacccat gatgttgata
    30121 tcaagcatat tttgccattc atcacggtga ccttcaacta atggagcggt tggcatgatt
    30181 cctgcattat tgtacaatac atcgattcga ccgaactttt ccatcgctaa atcgataact
    30241 gcttggactt gtgcaaaatc tcttacatct gctgtttgaa tatatagctc agcttctggt
    30301 aaagattctt ttattgcttt taggcgatct tctcggcgtg cagctataac tagtttagcc
    30361 cctttttctg ctaatagctt cgttgttgct tcaccgattc cgcttgaagc gcccatgatg
    30421 acaatcactt tttctgataa atcattcatt ctattctcct cctgttttga aaaatctctt
    30481 gcttatactc actatctctt atttcatctt actgttcaaa taatccgttc gacaggtttg
    30541 acgattttgg cagggattcc tgcaacgatg gtattgtctg ggacatcttt cgtaacagtt
    30601 gcattcgctg caacaatggc attctcgcca atcgttacgc ctggcagaat agttgctcct
    30661 gctccgatcc aagcattttt cttgatgtgg attggcttta ctgacaagcc tcttcgttct
    30721 tttggatcgg ttagatggtt caccgtgatg aggttgacac gaggaccaat caatactgaa
    30781 tcttctaaac agatgccacc taagtcaacg aacatcactt gacgattgat aaagacattt
    30841 ttccctatac taatatgttt gccgaaatct gtataaaaag gcagcagaac agtcaccgta
    30901 tcatctactt tgtcactaat gatattttct agccgtttgc ggatatctgc tggttcatgg
    30961 tacccagtat tcagcgctgc taaaggctct tcgttttcag cttggatctg atggatcact
    31021 tcaaagagcg gatccgttac tcgaatctct ttattgacga tttgtttttc gatgaggctt
    31081 gtcattttgc tccctccttt tatccttact ttataacgga cggcttatca tgtctaatac
    31141 ttataaaaca tacttaacta ttccttttaa ctatacttag tggtatgctt ttttaaaaac
    31201 aggagggaaa aaatggatat tcgtatactg acgtattttt tagcagtagc tagagaaaaa
    31261 aatatcagcc gagcagcaaa atctctgcat atcacgcaac ctactctctc aaaacaactg
    31321 aaagatttag aagaagaact tggtgtgaca cttttcactc ggggctcccg agagatacag
    31381 ttgacggatt ctggtcgata tctttattca aaaggaaaag aaatcttgtc gttggtagaa
    31441 aaaacggcga acaatttaac aaatgacgca atcatctctg gagaaatcta cattggcgga
    31501 ggcgaaacta aagcaatctc tttcatttct gatagccttc attcgttgat caaggcgcat
    31561 cctgatatcc agtttcattt atatagcggg aatgcagatg atatcataga caaactggat
    31621 agcggattac ttgattttgg actcgtaatc gaaccgacaa acaaacaaaa ataccagtca
    31681 tttcgactgc cagcccaaga tcgttgggga cttttgatac atcgaaatca tcctttagca
    31741 aagcaaaccg tcatccatcc gcaggaactg aaaactgttc ctttgatcat ttcccggcaa
    31801 acgtcagtgg acagtcaact agctgaatgg ctaggtttca gcatagatca gttatcgatc
    31861 gtcggaacat ataacttatt gtacaatgct tctttgatgg tcgataacgg gacttttggt
    31921 gcattagctc tagatgggat catcaatact tatgggacgg aaaaaatatt catcccactc
    31981 gatccgccat tacaagcttc actgaatctc atttggaaaa aagaccagcc tctttctcct
    32041 gctgctcgta aatttttaga tgtcctaacg gagaaataca gttaagtaga gtttttttcc
    32101 ttcgctttca gctttttaac aacgaataag ataagcaaaa gatccaacga ttaaataaca
    32161 taagtttctt aatttatttc ttccttttat ttctgataat tccagtaaat tactataatt
    32221 ataaaaaaag aattgacaaa acataactgt ctatataaaa ttgattacgt taacaataat
    32281 tcgtttcatg tgactagatc atactaggca tgaaaaatct ctgaggaaca tggtttccct
    32341 aggaggtttt tcgtgtctat ttttgtttgg ccgcaatcaa ctggtctagc aaacgtgaaa
    32401 accaaatctt caaaaagggt gcgcgcatga aaatcaaaaa gatctttaat caaaatgcag
    32461 tattggtaga tgacggtgga caggaaaaag tagctattgg aaaagggatt ggatttgaca
    32521 gaaaaagaaa tgatttgatt tttgatcgtg atgtcgaacg gatatttgtc atggaaccag
    32581 aaggtcaaat caagttgcaa aatttactta atcagattga tgaacgcttt ttatttgcag
    32641 cagaaacgat cattgatcat gcggaaactg ttttgatgga aaagttgaat gaacatgtat
    32701 tgatcgcttt gaccgaccac attgcttttt ctgcggagaa cattaataac ggtatcgtca
    32761 tccgcaacaa acttttaaga gaaatcgaag tgctttacag tgatgaattc agtattgctc
    32821 aatgggcagt agagtatctg acaaaagagc ttgggattcc atatacttat gacgaagcag
    32881 gatatatcgc aatccatatt cacagcggac gaagcggtcg gaacaataac catcgcagta
    32941 tccgtgaagt gacgattgta tctgacatca tccatttagt ggaatcagaa ttggaaacag
    33001 atatccattc agaaccattt tcactgaatt attcaagact ggtgaatcat ttacgactgc
    33061 ttctacaacg aacccatgcg caacaatatg ctgttttaga cacggaaatc gtagaaatgg
    33121 tcaaacgaaa atacccagaa agctataaga tttctaaaaa aatccgtgtg ttgctgacca
    33181 aagaatacca gctcgcgatc acgaaagaag aactgggtta cttggccatc catatcgagc
    33241 gtttaagaag cgcaatcgta caaacaaatg ttaacaatca tgaggaggaa aaaaattgaa
    33301 agcatatcta caacgaatgg gacgttccct tcaacttcca gttgcggtct tacctgctgc
    33361 tgctttacta gtagggatcg gaaactggtg ggcatcttat agtaacgaca ttgtcgctca
    33421 ttttttacaa gctggcggta acgcagtttt aggtcaatta cctttgctgt ttgccgttgg
    33481 gttagcacta gggatgtcag tggacaaatc aggtgctgct gccttggcag gtttggtcgc
    33541 atttgaagtt ccagtaaatg ttttaaaaac tgattcagta gctactttat tgaatatcaa
    33601 accagaatca gttgatccat cttttgctca gatcagtaac gtatttattg gtattattgc
    33661 cggattgatt gcggcagctc tttataatcg tttccacgaa gcaaaattac cgatggcttt
    33721 atctttcttt agcggcaagc gtctagtgcc aatcttggct gcctttgtca tgttgctgat
    33781 cagtgcagtg ctactgctcg tatggcctcc ggtttactct gctttagttt cttttgctaa
    33841 attcattttg agtttaggct ggggtggtgc aggtctttat ggcttcttca accgtttact
    33901 gatccctaca ggattgcatc aagcaatgaa ctccgtgttc tggtttgacg tagcaggaat
    33961 caatgatatt ggtaaatttt tggcttctga aggaacaaaa ggcgtaacag gaatgtatca
    34021 agctggattt ttcccaatca tgatgttcgg gcttccagca ggagcatatg ctatttacag
    34081 aaatgcacgt ccagaaaaca aagcaaaaac agcttccttg atgctagcag cagcttttgc
    34141 ttcattcttt actggtgtga cagaaccact tgaattttca tttatgttcg ttgcttggcc
    34201 attatatgtc ttgcatgcaa ttttcactgg gatttcttta gcagtgagtg cattcttcca
    34261 ttggacagca ggatttgcct ttagtgccgg atttgtcgat tacttcctat ctttgaaaaa
    34321 tccgattgcg aatcagccat tgatgttgat cgtccaagga ttagtaacag cagtgattta
    34381 ctactttggc tttaactttg ctatcaaaaa attccatctg atgactcctg gccgcgagga
    34441 agcagatctt tcagatgacg atactgcaac gactaattca gacaataaat atgcggcaca
    34501 agcaagcaaa atctacgctg cattaggcgg agccgacaat gtaacttcga tcgataactg
    34561 tactacacgc cttcgtttgc aagtcaaaga tacaggcctg atcgatcaaa ataaaatcaa
    34621 agccactggc gtacctggta tgaagatcat cgacggaaaa aatgcgcaag tcatcgtagg
    34681 tacagaagta caattcgttg cagatgagat ggcaaaacta catggtggtg ctgcagcacg
    34741 acctgcacaa acaaacaccg tggttaaaac agaaacattt gccggtgaaa cgatcgaaca
    34801 agatatttac gctatcgcaa atgggaaact gattccaatc actgaagttt cagatgatgt
    34861 attcgcggaa aaaatgatgg gtgacggcta tgctgtatta ccagaaaatg gagaaatctt
    34921 ctctccaatc gctggtacga tcacaaacat tttcccaacg aaacatgctg tcggaatcca
    34981 aacagatgct ggtatcgaag tcttgcttca tatgggcatc aacactgttg acttaaaagg
    35041 cgaaccattc actttatatg tagaagaagg tcaaaaagtc gcaagaggtc aattgattgc
    35101 tttagttgat cttgccgcta ttcaatctgc aggcaaaaac acggatatgg tcgttgtatt
    35161 cacaaatggc gacaaagtcc aaagcttaga aatcgaaccc gcacgtgatg taaaagcaaa
    35221 tgataaaatc ggctctgttt ccaacaaagc ctgagccatc gtcttcccca gactttttta
    35281 tcgataaaca aacaggagct gagacaaaaa tcttgtctca gctccttttt ccgaataaaa
    35341 acggcgttct cctgccaact ccgcggtaat aagtccagct aaacgaaaca tatgaagagc
    35401 tattttttat ttgactcttc ttattacttg atgcagaaca gctttttcac aaaatttttc
    35461 ttcatactca aaactaatcg gctttttcac aacctcttta tacattattc ctgatccaat
    35521 atttctttta atgctgcttc aagagaagtt aatggttttc ccaatacttt ttctaaatca
    35581 tcagaggaaa tagctaactg atccttttta atatcatgtt ggatcgctaa aaacatgtca
    35641 gctgcttctt gagggaatcc ggcttctttt agattttcga caaacgctgc atcacttgct
    35701 tcaataattt ctaactcttt acctgtcgca cgctctaatg ccttggctaa ttcttcatag
    35761 tcagttagat tgccggataa ttctaagatt tctggagagt cactttctac tagtactttt
    35821 gctgcagctt ctgcatattc acgtttcaat gcccaacctg tttttccatt tcctgcagca
    35881 taaacgaatt gaccagtttt taaagcctgc ccaatgatag gtaattcatt ctctacatac
    35941 caattattcc gtaaaaatgt atgggaaata cctgaatcct taatcatttt ttcagtgaat
    36001 tggtgatctg gcgctaatac actgatcgag tgatcggcat ctgcaaaact tgtataagcg
    36061 atataagata ccccagctgc ttgtgctgcc ttcactacat tcttatgttc ggcttgacga
    36121 tttcctggaa cgccagagac aaaaagcaat cgatctattc ctttcaacgc ttctttcatt
    36181 gagtctaaat catcataatc accaatacgt atattaaatc cagcttcttt cagtgcttct
    36241 cctttttctt cactacgtac tagtacatag atttcatttt gtggaaccat cttttttaag
    36301 aattccagcg cataaccacc aaaacctcca gttgctcctg ttactaaata atgcatattt
    36361 gccactccct attcttttta tttgttaaga tttacagctg tacatttata aattacacct
    36421 ttatcataaa gagtgtggta agataagtca agcaaaaaaa atgaaaggat gacaactgat
    36481 gaagtattct ctgcaattta gtgatgcaat ccatgtactt gcctatattg aaatgtttca
    36541 agatacccga tcactttcta gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa
    36601 tgtacgtaaa atcatgagtc agctcaaaaa atccggtttg atctttacaa aaaccggaaa
    36661 accgcagcct tcccttgcca aaagtccaga tcagatttct ttactagatg tctatcgcag
    36721 catcgaaggt aatacacagc ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt
    36781 tggtgcgaat atccaagaag ctttagaaga aagctacgct cggctgcaga aaaaggcaga
    36841 agaagaaatg gctaagatca cacttgatag tctgatacat aaaatagccg ttttggaaaa
    36901 agaaaaaaga ccagaaaaca caggcctcgt agagagattt ttataattct ctctactgtg
    36961 cctgcatttt tctgatcgga atcgtctaca taaattcttt tattctccgg gggacaaaag
    37021 ccttgtcatg cccctttttc ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc
    37081 ttgataaacg gtgttcaagt agctgaccct cggcattagt tctgaaaaat gcaaaatatg
    37141 gaaagctatt tcccattttt cctctctaat gctcaggtca aagcgctact ttcacgacct
    37201 ctatattatc atggtttatt gactaccggt atccagattt ctcccatata tttcccgttg
    37261 gtaactccca tttcgactac agcatttgga cccccaacat aggcgtaatc ttctacttct
    37321 cttaaaacat gcccaaaggc cgtattcgtc aaatcatttc ttaattcttc aggcgattct
    37381 gctaaagcgc ttacaacaag atacgttcct tttgggaatg ggatgattcg cgtagtgttt
    37441 ggaacatcct gactcgtttg aacaccgaca tagtacatca ttttcccgtt atagggttcg
    37501 ttgactgtaa agaaataatg attttcagca gcttgtttca atgcatctat tgttccatta
    37561 ccgataactt tttgctcaaa tgcttgtttc tctgcagcca aaccgacaaa atccgtattg
    37621 ctactcttca attccacacc aatacccgtc accgtaaaat ctggtttttc ttcgatccta
    37681 tactttgcca tttctcccac cctttctatt atattaagtt catcttacat aaaattaaag
    37741 ggaaatgaca cgattacgct cttttttcta gggatatttt tattttttaa ctattttttt
    37801 gctttcactg caatccaaat ctcactgtaa acattactga aatcctctcc ccattttgta
    37861 aaagagatct cgggagcttc gactaaccca taatctgaag aaggaagcca ttctgcatag
    37921 attttccccc aagtatcttg taaagtttgg ggaaatggcc ctttattagg aaaaatggcc
    37981 caagtgtgtt cttctatagc aagcatttct aaatcagcgt acggattttc ttttgtcgta
    38041 gcaaagccaa tcatgtgcat caaactgccc ttttcttcca agcggtctcc atcaaatcca
    38101 taagaaacgt tgattacttg atttggatat aaatctccta acttatgcat ctcttccctt
    38161 tgagtctccg tgatcgtctg cgctaacttt tgtatttctt gattgactcc ttcaaatttg
    38221 acaggaactt ttttcgccac acctgccaca taaaactgtt ctttcttttc gatacgaaat
    38281 tccatcgatt ttcctcctct gacatcaata aaaaatgtca agcgtggaaa agatttttgt
    38341 atgccgtatc tactgatttc tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa
    38401 aaaccttcga ctgtttcata tccatatcta aacgccgttt ctgtcactct tgatccatct
    38461 agaagatcgc gattagccaa agccaatcta cggtttttga tatagtccat tagcgacatc
    38521 cctgcaataa acgtaaagat gcgttttaat tgatattcag agattcctgt attttcggct
    38581 attattttat aggaaagtcc ttctgtcaaa tgttcttcga tgtaatcgat caattggttc
    38641 agatctttaa gcatatttat cttcctcctt gattgccttt ttatcatatt atcaaagaaa
    38701 gtcaattact tgatgaatca ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc
    38761 taaagaaaat tctcaagcag cgtttcgtaa cggataattg gtattatctg cctatctatg
    38821 ttttaccttt agaaaaataa attttgggga agacagattc taagtaaaca aagcggtaaa
    38881 aatatctttt attcccttag ttaatccgct tactacctac tgtacaaagc aaattgttgt
    38941 tcaaactgct gatcagaacg ctgcttggat ttttctttgc gatacatcat acctctttta
    39001 tcaattggaa caattattta tttctaacaa aatcacttca tttttgtcag ataaataaaa
    39061 aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca cattctctaa
    39121 gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc aaatatgtaa
    39181 aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt gtttcatgaa
    39241 aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg gaacgcggtt
    39301 acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag tacttgtccg
    39361 caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa aagaccaaca
    39421 tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg gctgctgtct
    39481 tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc gaactctcgt
    39541 cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag attcttatag
    39601 aacatccgca ctttatcgaa cgcttcaaag ttaagatgcc atttcatgca ataaattatt
    39661 cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa aacaaaaaca
    39721 ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca gttgaatcgc
    39781 ttattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg attgctactc
    39841 tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata gaggactgtt
    39901 attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg caaatgttct
    39961 cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc tactctataa
    40021 aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa aaaatagata
    40081 aaaaactaaa gaaacggtaa agaaagacga tttcaatgaa aaatagcaag taaacgataa
    40141 caaccatcct aaatccacaa gaaatacagc agaccttgct ttaaaaacac gaatcatgca
    40201 aaggatgaag taaagaagac ttaacgcgta cccaatatta gtcatttgac tattaggtac
    40261 gcgttttgtt ttataagtca tgacaaattt ccttcgttgc tggatagatt ttccggcatt
    40321 gttgatagat tttatgatac ttttctacat tttgtaagag aggttccgct gcatctgtat
    40381 attggataaa attctgacaa caactttcag gtgtgtcgaa ccaccctaac cctagtgcag
    40441 caatcattgt tgcgcctaat gcaggtcctt cctccacact taaacaatag accgttgcat
    40501 cgaaaatatc agcttggatt tgtagccatg caagattttt cgcgcctcct cccaccgaaa
    40561 caatacgtgt aaatctgcga cccgctagtt ctttacgttt caatcccacc attgacaaat
    40621 gctcattaaa aatttagaat attcacaagt ttaacgacaa ttatgaaaat ttatgtcgtg
    40681 ctaaaggagt tttgttattg gatgaaaagt tagatataaa aactgcgatc aaagacacgt
    40741 tacctaccgt tttcggttat atcggtattg gacttgcatt tggtatcgtt gggaaagctg
    40801 ccggatttca tccactagtc gtcacgttga tgtccctgct ggtctatgct ggttctgccc
    40861 aatttatcac agtcagcatg cttgctagtc acagcccatt gctttccatc gttttctcga
    40921 cctttctagt caattcccga atgattctga tgagtatgac gattgctcct tatttcaaga
    40981 aaaatcggct gcttcaaaat ctgctgattg gcacattgct gacagatgaa agtttcgcac
    41041 tgggaatgaa taaattgaac tatactggac aaaagttaaa ctttcgttgg atgaatacag
    41101 ctaattggat ttcttatttg acgtgggtgg gttcttctct tgttggcgca cttttaggga
    41161 attttattac tgatccgaaa aagtttggtt tggatttcgc catcgtcgct atgtttatcg
    41221 ggttacttta tcttcaagtc atttctgata gaaacacctc taaacgatta cagttgatcc
    41281 tgattggtct cactttgatt ctggtatatg tcggactgat ttttatccca agcaacttag
    41341 tgattgtagt tgtaacactg attgggtgcg gattgggggt gtggatcaaa catgccttct
    41401 tttgagttta cattgctgac tattttaggt tgtactttag caacttggct ttctagggtc
    41461 ctaccatttg tcttactgaa aaaattcgat ttaccgcaac cgttgcttga gtatttaagt
    41521 tttgtcccga tcgtcattat gtctgcttta tggttcagca gtctatttac ccaaaatatc
    41581 ggacatctgc cgcagatcaa tctcgaaaat gctttggctt cagttcctac acttttagct
    41641 gctattcttt caaaaagctt attggtgatc gtcttggcag gaattctttc cctttcgttg
    41701 attcgcttat tataaataaa aaaacaagag cttatggcaa attactgtca tgcgctcttg
    41761 tttttatcaa agtcaaataa gaaaaaaatc ggcgtattct tgcttatccg ttgaacggac
    41821 cactaacaac gacttttcct aacatatgtc ctgtttctac gtctttggtt gcttgtttca
    41881 agttcttcgc attcatccca tctgaataga ccttgctcaa tgtactgcgg ataatccctt
    41941 catttgctaa agcagcaatg tgcgctaaag cttctccttg cgtttcgatt tcatagttat
    42001 agtctgtttt agcgaacata tactcccagt cgaagctgat agattggttt ttccaaacac
    42061 ctatatctaa cggctctttg atccctacga tcgttcccac atgtccaaat ggtcggacaa
    42121 ggtgcttgat ttgatcaaag tagcgggtaa tatcgaataa tacagcgaca tactctactt
    42181 gatcgattcc taattcacga aaacttttac ttaagtcgtg atgataatcg attggatagt
    42241 ctacacctgt ttttttcagc cattcaaaat tcttcggact tgcagtcgcg tagacttcta
    42301 ggccagccca atgcgctagt tgattcaaaa tcgaaccgac gcccccggat ccgttgatca
    42361 ctaagatttt tttcccgcga ttggcatttt cttctggagt tagaccaaat ttttcaaata
    42421 acaattcata agcggtcaat gaggtcaacg gcaaggctgc agcttcttca tctgaaagat
    42481 tttttggtgc aagagcgaca atacgttcgt caactagttg gtattcttga ttgcttcctg
    42541 ctcttgttgt tgtacctgcg taaaaaacgc gatcgccgac agaaaatttt tgcacttcgt
    42601 cacctacagc aacaactttc ccgactccat caaaacctaa gatacgtaaa gcatttctta
    42661 tcccgttatc ttggcgaagt ttcgtatcga caggattaac agatactgcg ttgactttta
    42721 ccagaagatc ccgtgaactg ggtacaggta tctgttcttt ttcatcaata aagctttttg
    42781 gatcatcgat cggcaaccct tcataaaaac cgactacctt cattaattcc ttttctgaca
    42841 tacgaattcc tccttagctt catcaagttg gagcatccat acagtgttca tttcaatata
    42901 tatagtatgc tatttgttcc acctttttta ttcatcataa ttattctctt acttaaagta
    42961 aactatttgc acttttttgg ttaaaaaaga ggttgcgaca agacttttgt cacaacctct
    43021 tataaacggt gttctcctac caactcttcg gtcacaagtc aaaatattca tcaaacatga
    43081 gaagctgttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc
    43141 tgatataaac ggtgttcaat caactgaccc tcggtattag ctcaaaaaac gacaaaatat
    43201 gaagagctat ttttgtttgg ctgttcttat tacttgatac aggacagctt tttcacgacc
    43261 tctaattcac ggtgttcaag caagaaatca ttgttcactg aaaagcgttg gatgttcttt
    43321 ttcaaaggtc tctaagatct ctcgtacttc atcagtagat ttcgtattca tcaactgaac
    43381 tcgcaagtca tttgctcctg gaaatccttt gatatagatt ttaaagaaac gatgtagtcc
    43441 tgtgatggaa cgcgggatga tttctgaata atgatcttgc agatctagtt gcagacgaag
    43501 tagtccgatc agttcttttt ctgtatgttc cctcgggtct ttttcaaatg cataaggatt
    43561 cttgaaaatc cctcggccaa tcatgatgcc atcaacgcca tatttctcgg ccaattcttc
    43621 tcctgtctgg cgatcgggga tatctccatt gatcgtgatt agtgtttgtg gagcgatttg
    43681 gtcacgtaaa gcaatgattt taggaatgac ttcccaatgt gcatctactt tactcatctc
    43741 tttacgcgta cgcaaatgga cagaaagatt ggctatatct tgatgaagta ggtgactgat
    43801 ccattcttcc atctcagaca tttccgtata gccgatacgc gtcttgacgc tgacaggcaa
    43861 accgccagct ttagctgctt caatcaattg tgccgctact tctggacgaa ggatcaagcc
    43921 actgcctttt ccacgttctg ctacattagg gactgggcag cccatgttga tatcgattcc
    43981 ttggaagccc atttctgcca tagcaatgct catttctcta aaaaattctg gcttgtctcc
    44041 ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaat gttaatcttc ctcgtacact
    44101 gtcttttcca tctggatgac agaaactgtc tgagtttgta aactcagtaa agaagacatc
    44161 cggtgctgct gcatgcttta cgacatggcg aaatacaaca tctgttacat cttccattgg
    44221 tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa ttagtagtca tttatttgtt
    44281 actctccatt ctatcaagca gtgaagtcgc aatctttctc aaaaacattg tcattatact
    44341 aggaaacaaa aaaagaagca aagtgaacag cttaaaagat gtttctgatt gagcaaaaaa
    44401 aagactgtga caaaaatctt atcacagtcc attttccgga ataaatggtg ttcaaaagct
    44461 tgcatttgcg gtaataagtc caaccaaacg aaaaatagga ggagctattt ttgtttggct
    44521 gttcttatta cttgatgcag aacagctttt tcacaacctc tccactaaac ggtgttcaat
    44581 cagctgaccc tcggtattcg ctcaaaaaac aacaaaatat gaggaactat tttcccgttt
    44641 gtttctctaa tactcaggtc aaaacgctga tttcacaacc tcttttctaa cttctcaatc
    44701 ctcttccttt attgatcaaa taccaagtaa cggcgtacaa aacaacgatg aataacacaa
    44761 gtacgatcat cgatgttgct actggcacat cgatcgttcc aaggaatccg taacggaaac
    44821 cggagatcat ataaacgatc ggattgactt ttgaaacagc ttgccaaaag ggcggtaaca
    44881 tagagatggc atagaaaacg ccacctagat aagttaacgg ctgcaagaca aatgtaggca
    44941 cgatcgatac atcatcataa gactgagcaa aaatcccgtt gagcagtcct gctaatgaaa
    45001 acaagattgc cgtcatcaat aatgtaatga tgaccatgaa ccatgaatac acatgaagtg
    45061 gtacaaagaa caaggaaatg atcgttacga gagagccgac gagaatactg cgacccacac
    45121 caccaatcac aaagccccaa ataatcacat gcgtaggaac aggtgctacc aagatttctt
    45181 cgatattttt ctgaaatttt tgagagaaga aggacgaaga aacattggca tacgaactag
    45241 tgatagcaga catcataatc aaaccaggaa cgatgaattc catatatgaa aaaccaccca
    45301 tgtcaccgat ccgcccaccg atcatttttc cgaaaataac aaaatacaag gatgtcgtga
    45361 tcactggcgg aaccagtgtt tggacccata tacgcaaata gcggttcgtt tccttgatag
    45421 ctaaactttt taatgcagta aaataaagat taaacatttt tctcctccgt atgattattt
    45481 tcttctgtga ttttcaagaa cagttcttct aaacgattcg acttattcct catagaaaga
    45541 actttgattc cttgtcttgt aagctgatcg aagattccgt tcaccccttg atttcgttca
    45601 acttcgacag cgattgtctg ctcatcttca aatgtacaag catatccttc aatcacaggt
    45661 ttggtatcat atggcgctaa atcaaagata aatgtttcaa attgcagttt tgacagaagc
    45721 tctttcatac ttgtattctc gatcagttca cccgattgga tgattccgat gttgcgacaa
    45781 agcatttcag cttcttctag ataatgcgtc gtcaaaatga tcgttgtacc attttcgttc
    45841 aattctcgta gaaactccca catctcgcgt ctcaactcga tgtcaactcc tgctgttggt
    45901 tcatccaaga tcagcaaacg tggttcatgc atcaatgccc tagcaatcat cagtcttcgc
    45961 ttcattcccc cggaaagcat acgtgcacgg acatttctct tttcccaaag attggactgc
    46021 ttcaaatatt tttcgctgcg tttcatcgct tctttgcgag agacaccgta gtagcctgct
    46081 tgattcacga cgatttgctg gaccgtttca aaagggttaa aattgaactc ttgtggaacc
    46141 aatccaatct gttgctttgc tcgaactaga tccttgtcca gatcataacc gaagacattc
    46201 acttttccag atgttttatt gactaaagaa gtaataatcc cgatcgttgt agacttccca
    46261 gctccatttg gccctaatag cgcatagaaa tctccttctt tcaccgaaag atcgattccc
    46321 cgcaatgctt cgaccccact tccatagatc tttttcaaat tatttatttc aagcgcattt
    46381 gccatttatt tcctatctcc ttatagacat gattcttaat tgtatcatgg aatacagcag
    46441 atgaaaaaac aacttggctt ccacacaaaa acacacctgc taacaaagag gagatgtgtt
    46501 ttctagttca tgatcatctt cgatagaata actatctaaa caactctgga acttttaaaa
    46561 tcataaaagt accgacagca actactacga tgacatatag aagatcctta taactgaggt
    46621 tcttgtcttt tttattcatg ttaagctttc ctccgtcctt ttatcgaaag cttttttctt
    46681 ttattttaac cgaaataaat aaaatggaaa tagttatttt caatttctgt tttcgctgtt
    46741 tgttttacgg agaactttgc catggaacgt gcgtctcgtc gctgatgcta agttttttcg
    46801 ataaaagctg ctagaaggaa gacagcgtgg tttattttgt atcgtctttt gctaaatctt
    46861 ccaagatcat ctggacagca gactcgttcc cattgaattc tgccactttt ttggcatcaa
    46921 tgtacttctt ttgataatcg tctttttgga cccacgcatc atgcttcgtt tgataaagtt
    46981 aatcaaacgg ataccactga cgctgttcat cgaacaacaa actttctttt tgggcctgtt
    47041 caactatgga tttaggataa ttcaaaactt tcagcaaggc aatcgcattt cgggtccttg
    47101 aaggtccttc ttttaatttg aaatcaaaac tgattccttt tccttctgtt acttgttctt
    47161 caaagtggac attctcacaa taattcttta aaatctctgt caattcgata tcatgtgtgg
    47221 cgacaaatgc taaagatgga tactccgcaa gccaccgaac gactgaagcg gaagaagcaa
    47281 tccgttcgat cgtatttgtt ccttttaaaa tctcatctac gaagcagtag cagcgttctt
    47341 tggaagccac ttggtccagc aaacgtttga tcgatttgat ttctgaaaca aaataactgt
    47401 cgccttcaaa taaatcatct tcgattgcca tggaagtcaa cacatgtcca ggttgcaggg
    47461 taaactgttc tgcaatcgct gtttggatcg tctgtgctag aatacagctg atggctatac
    47521 ttttgacata cgtagatttt cctgatgcat tggaacccgt gactaatgta ttcttctgcc
    47581 agttgactgg attaactacc gcttctttta acaatggatg atagatatct ttcgctgtca
    47641 caccgccctc agtaaactct ggtcggcaag tgatcggcat ataggtgcga taattcaaaa
    47701 cggctgctgc aacttccaat tctcccagca attcccacag cttgatggct tcagttgaat
    47761 gcttttctaa tttagtgaga acgaaatgat aggagataaa tggcagcatg aacatgatgt
    47821 tgacatattc aaaaatcaat tctgcttcac tcccgctctt cattcgaaat gaaaaaccta
    47881 atttagggat gtctcttaat ggtttagttt gctgtttcag ctctttttga agtggtgtcg
    47941 tcatttttgc taactgattg gcggctgaaa tcgttgaaac caaataactc atgctattca
    48001 gctctgtttc tagtgtttgt ttcttcacta gataatagac cgtattgaat actaagccac
    48061 cgattgctag atagattcct agtacttgcc ctaaaagaag caaacctaca cccaccagcg
    48121 gaagcaagcc aagaaaaata aataaaccta cgcgtccaat ctttttactt tttccatccg
    48181 ctagatattg ttttgcaaaa ttatgatctt tttttcctaa acgagcgaat tgatattgga
    48241 ttctttctct cagttgagga ttttcctgat aaaaagcaat tagtttttct aactgataat
    48301 cttctccaaa atcaaagcta cgtaaacgct gatacaacgc ttctgaacct acactagaat
    48361 aggtactgtt gatcccttca aatacttcga acatatccaa gtcataccaa gtaagatcgt
    48421 cgatttcact atcccatgaa cggaattttt tctccgtgag ccacgcatcc ttcaaacttt
    48481 cttctttatc aaatctcggt tgataaggaa tttttcccca attactacga actgtttcct
    48541 ttactttcaa acgtgaaaaa atgtctaaaa caactaataa aataacaact gcaataataa
    48601 ttaaaacgat aaattctctt tggcccatat ccctctccgt tccttgattt gttagtttta
    48661 gtatagcact gttgcgctat ttgaaaaggg ggtacaaaaa taactgtctt cgttacctaa
    48721 ttcgtatcaa tcacaccaaa ataaaaacac cttccaaaaa agaagatgtc tttaaaacga
    48781 gatacaattt tttgtcattc acttcaggta aattagagcc aattaaaccc tccgcaactt
    48841 gacggagatg gagaagatgc ccccgtctaa tctagcggtc atctctcctc ccattccttc
    48901 caccaatttt tttgacaggt aaagcccgat tccgctagat tccgtatttg tgcggctggt
    48961 attttcggta taaaaacgct ggaagacttt ttcaacagag gtttgcgctc ctggtttgat
    49021 aggattggcc actgttaatc gaatgaatga atcctctttt gaaaatgtca cctttgcttg
    49081 tccatctgca taacgcaata aattctgtat gatattttgc agcactcgca gcaacatgag
    49141 cggatctgct aatacagttg tatttatcga gtgttcagcg aacgctacct cgatattttt
    49201 ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc acctctttag aaaaagaaag
    49261 cgtatggaga tccatttctt tttgcttcgt ctctattcga gcaagatcat aaaactgatc
    49321 cgtcatactg agtaatcttg caacagattg tttgatgatc gttaaatact tctcccgttt
    49381 ctccgtatcc tctacctgtt ccagtaattg tacataaccg ttaatagcag tcaaaggagt
    49441 tctgagatca tgagaaaggc cgacgatcga tagtctgatg ttttcttcca tttcttgaaa
    49501 tgcttgtcct ttttgttcat tttcatccac ccatgtattc agtgcatcta ctaggtccat
    49561 tagattcttt tctcgaaatt cgactgaaag acgaccacca tattcttgtc gttcagaaag
    49621 agtggcgatt ttcttagtta atctcttgat ttccctttgc aggaagaaaa taaaacttcc
    49681 cataaagata acaacgagaa gcaacagcca cttaatcaaa tctaaacccc actccccaca
    49741 ccgtatgaat atacacagca tgtgcctttg ctagtttccc tcgaagattg gaaatatgca
    49801 tattcactgt tttctcacta tcaaaatatg gttcttgcca aactgtttca taaagatttg
    49861 ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt cagcagatcg aattctcttc
    49921 ctgtcaaatg gacagccaca ccattcacat ttacatccct cgactctttg tccagtaaga
    49981 tttccttgta atagatgcgt tgcgacagat cttttacttg gacagaagtc tgatgacgaa
    50041 gttggatgcc gattcgtaac aacagttctt cgatatcaaa tggtttcgtc acatagtcat
    50101 ccgctcctgc tgccaacaag gaaactttat ctctttgatc gctttttgct gaaagaacga
    50161 tgatcggaac attcgatgtc tgtcggattt ctgctaacaa tgcttcacct gaaagccctg
    50221 gaagcatgag atctagaagg atcaaatcga caggatatgt gcttaacaat aattttcctt
    50281 ccgtacctga ataagcatca agaagtttgt atctctgttc aagaacctcc tccagtaaag
    50341 aatgaacagc ttcatcgtct tcgataatca aaatagtttc cagcaggaac acctacttaa
    50401 tgtcagattt ttggaaaata aacattccta gagctgatga taccacgatt gttactagac
    50461 tgaccagaat cattttcggt gcttgcgaat aagttgcttg ttgcgcaaag tccgtataca
    50521 tgagccacgt ttccgtaaac cactccgtta gaaattccaa gcctttgatt tttgcgaaaa
    50581 caccgggaac gaaggccgtt cccatgatat aaagaaaaga aagaagcaaa ctgacactgg
    50641 tacttctagt gatgaatgtc aaagccactg gaacagataa tgtcgctaat atcagcagaa
    50701 gcaatacagg aaatactttt agaacataat ttccatagtt tttgattccc tcaacttcac
    50761 caaatccgaa agcaagtgtg taagcaatca tggctagccc cgtaatcaaa accacgccaa
    50821 gtgttgtgat caaaagcgta acgagaagtt tcgacacata ataagtcatt cgattccgtc
    50881 ctaaagataa cgtattgcga atcgtaccgt tttgaaattc ttccccaata taaaagcacg
    50941 caaatgctgc gaaagccaga agaatccatg cagccatttt ttccaccatt aaaatccctg
    51001 ttgcaccgtt gatgactgta tccgttgctt ggtttaattc attgccgtat tttgtatgca
    51061 tataccattc gttccaagaa taaaaaagcg gaagtacgac catcagtcct agtgagaccc
    51121 acattttacg gctcttccat acttttattt tttcagcagt taatagatta aacatgtgga
    51181 ttgcctccag ttaattgtaa gaaataatct tccagttttt gcccaactag gtggatacca
    51241 tccacaggaa cttttgcctc taccaatgct tgattgatgg ctgctacttg gtcaattcct
    51301 tcaaaaagct ggataacacg gctagattgg acaaaataat ccctataacc aagagagtcg
    51361 agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt ttataaattg acgagattct
    51421 tgtgccaatt cttctttcga taattctttg atcaagtgtc cttcatgcaa aataccgtag
    51481 tgagtggcga tctgtgacaa ttcatctagc aagtggctag aaagcaagac ggtgatgccc
    51541 cgttctttta ccaaacgttg gatgatttct cgcatttctg ccatccctgc tggatccatc
    51601 ccattcgtcg gttcatccaa aatcaaaaat tctggattcg tgatcagtgc atttgcaata
    51661 gctaaacgct ggcgcatacc taaagagaaa ttctttgctt tctttttccc tgtattttcc
    51721 aatcgcatca ttttcagcag gtcttcgatt tctctatccg acacaccgcc gttcgctgct
    51781 tggatacgta ggttatctct tgcagttaat tctggataaa gagcaggggt ttcaatcgat
    51841 tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc caaataattc gatcgaacct
    51901 tcatcaatcg agatcaaacc cataatagtt ctcataaagg tagacttacc cgctccattc
    51961 tccccaatca aaccatagat cattccacgt ttgatttcga tcgatacttt atctaatgct
    52021 ttggcagcac cgtatttttt ggttatacca gttgctttca agaccgtttc atttccaaaa
    52081 tccatactta tccttctttc ttattgataa gtgaagtata gttgacgggt ataaagaaag
    52141 cagccagaaa tgtataaaga aagtgtaaat tttccaattg aatttcaaga aaaatcagac
    52201 tgtctcgcag acgtggttga gttgtctgcc aatcaaagag cgcaccaaac agtgctaatc
    52261 caagtggact ggccaccatg gtaatcgttg ttatcgttga catcagacgt ccttgtatat
    52321 gcgttggaac ttctcgctga agccagacca tataaaaagc aaaagaaaac attaatcctt
    52381 aaaaacggac ttatgaaaaa taatacatgc cgtttgggat ttacaattag ctaccaaata
    52441 agaggttatg acaaaagact tttcacaatc ccttcttccg aataaacggt gttctcctgc
    52501 caactcctcg gtcacaagtc acaatattta tcaaacatga gaagcaattt ttgtaaattc
    52561 cttcttattt cttgaagctg accacttctg tcacaacctc agaagcggat aaataatatt
    52621 gtttgatgat actttatctt tttcccgttt gtttatagat cgaatgatat tatagaccaa
    52681 gaatcctacc tattaacata gctaaaaaca caaaaaaggc actttccaaa ttgtggaaag
    52741 tgccagaaaa gttaggatta aggtaactgc gctcagcatt gcttcgccca gtacgattct
    52801 cgtaactcgt gaactcgaac gataatcgca attaacgttt tgagaactgt gaagctttac
    52861 gggctttctt aagacctggt tttttcttcg tatcattgaa taagtttgaa aaacctt
//
LOCUS       DAJSMI010000004        51747 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJSMI010000004 DAJSMI010000000
VERSION     DAJSMI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.004"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            119..490
                     /locus_tag="ON430_000191"
     CDS             119..490
                     /locus_tag="ON430_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR3218141.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(764..1456)
                     /locus_tag="ON430_000192"
     CDS             complement(764..1456)
                     /locus_tag="ON430_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3218142.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1459..2244)
                     /locus_tag="ON430_000193"
     CDS             complement(1459..2244)
                     /locus_tag="ON430_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3218143.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2481..3881
                     /gene="lacG"
                     /locus_tag="ON430_000194"
     CDS             2481..3881
                     /gene="lacG"
                     /locus_tag="ON430_000194"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCR3218144.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIDFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3906..4223
                     /locus_tag="ON430_000195"
     CDS             3906..4223
                     /locus_tag="ON430_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3218145.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4236..5909
                     /locus_tag="ON430_000196"
     CDS             4236..5909
                     /locus_tag="ON430_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCR3218146.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPVPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5923..7554
                     /locus_tag="ON430_000197"
     CDS             5923..7554
                     /locus_tag="ON430_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCR3218147.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8045..9610)
                     /gene="guaA"
                     /locus_tag="ON430_000198"
     CDS             complement(8045..9610)
                     /gene="guaA"
                     /locus_tag="ON430_000198"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR3218148.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9669..10592)
                     /locus_tag="ON430_000199"
     CDS             complement(9669..10592)
                     /locus_tag="ON430_000199"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR3218149.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10788..11393
                     /locus_tag="ON430_000200"
     CDS             10788..11393
                     /locus_tag="ON430_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3218150.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11701..12717
                     /locus_tag="ON430_000201"
     CDS             11701..12717
                     /locus_tag="ON430_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3218151.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12858..14159
                     /locus_tag="ON430_000202"
     CDS             12858..14159
                     /locus_tag="ON430_000202"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCR3218152.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14205..14867
                     /gene="deoC"
                     /locus_tag="ON430_000203"
     CDS             14205..14867
                     /gene="deoC"
                     /locus_tag="ON430_000203"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR3218153.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14888..15286
                     /locus_tag="ON430_000204"
     CDS             14888..15286
                     /locus_tag="ON430_000204"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR3218154.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15352..16434
                     /locus_tag="ON430_000205"
     CDS             15352..16434
                     /locus_tag="ON430_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3218155.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16646..18208
                     /locus_tag="ON430_000206"
     CDS             16646..18208
                     /locus_tag="ON430_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218156.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18201..19334
                     /locus_tag="ON430_000207"
     CDS             18201..19334
                     /locus_tag="ON430_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218157.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19334..20290
                     /locus_tag="ON430_000208"
     CDS             19334..20290
                     /locus_tag="ON430_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218158.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20389..21555
                     /gene="deoB"
                     /locus_tag="ON430_000209"
     CDS             20389..21555
                     /gene="deoB"
                     /locus_tag="ON430_000209"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR3218159.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21579..22397
                     /locus_tag="ON430_000210"
     CDS             21579..22397
                     /locus_tag="ON430_000210"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3218160.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22414..23121
                     /gene="deoD"
                     /locus_tag="ON430_000211"
     CDS             22414..23121
                     /gene="deoD"
                     /locus_tag="ON430_000211"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3218161.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23419..24105)
                     /gene="gpmA"
                     /locus_tag="ON430_000212"
     CDS             complement(23419..24105)
                     /gene="gpmA"
                     /locus_tag="ON430_000212"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR3218162.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24277..24858)
                     /locus_tag="ON430_000213"
     CDS             complement(24277..24858)
                     /locus_tag="ON430_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3218163.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(24991..25674)
                     /gene="rpiA"
                     /locus_tag="ON430_000214"
     CDS             complement(24991..25674)
                     /gene="rpiA"
                     /locus_tag="ON430_000214"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR3218164.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            25993..26406
                     /gene="rpsL"
                     /locus_tag="ON430_000215"
     CDS             25993..26406
                     /gene="rpsL"
                     /locus_tag="ON430_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR3218165.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26490..26960
                     /gene="rpsG"
                     /locus_tag="ON430_000216"
     CDS             26490..26960
                     /gene="rpsG"
                     /locus_tag="ON430_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR3218166.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27054..29141
                     /gene="fusA"
                     /locus_tag="ON430_000217"
     CDS             27054..29141
                     /gene="fusA"
                     /locus_tag="ON430_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR3218167.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29313..30500
                     /gene="tuf"
                     /locus_tag="ON430_000218"
     CDS             29313..30500
                     /gene="tuf"
                     /locus_tag="ON430_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3218168.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30907..31215
                     /gene="rpsJ"
                     /locus_tag="ON430_000219"
     CDS             30907..31215
                     /gene="rpsJ"
                     /locus_tag="ON430_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR3218169.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31246..31875
                     /gene="rplC"
                     /locus_tag="ON430_000220"
     CDS             31246..31875
                     /gene="rplC"
                     /locus_tag="ON430_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR3218170.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31903..32526
                     /gene="rplD"
                     /locus_tag="ON430_000221"
     CDS             31903..32526
                     /gene="rplD"
                     /locus_tag="ON430_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR3218171.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32526..32816
                     /gene="rplW"
                     /locus_tag="ON430_000222"
     CDS             32526..32816
                     /gene="rplW"
                     /locus_tag="ON430_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR3218172.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32855..33688
                     /gene="rplB"
                     /locus_tag="ON430_000223"
     CDS             32855..33688
                     /gene="rplB"
                     /locus_tag="ON430_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR3218173.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33731..34009
                     /gene="rpsS"
                     /locus_tag="ON430_000224"
     CDS             33731..34009
                     /gene="rpsS"
                     /locus_tag="ON430_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR3218174.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34032..34379
                     /gene="rplV"
                     /locus_tag="ON430_000225"
     CDS             34032..34379
                     /gene="rplV"
                     /locus_tag="ON430_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR3218175.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34393..35049
                     /gene="rpsC"
                     /locus_tag="ON430_000226"
     CDS             34393..35049
                     /gene="rpsC"
                     /locus_tag="ON430_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR3218176.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35052..35486
                     /gene="rplP"
                     /locus_tag="ON430_000227"
     CDS             35052..35486
                     /gene="rplP"
                     /locus_tag="ON430_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR3218177.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35476..35664
                     /gene="rpmC"
                     /locus_tag="ON430_000228"
     CDS             35476..35664
                     /gene="rpmC"
                     /locus_tag="ON430_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR3218178.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35689..35955
                     /gene="rpsQ"
                     /locus_tag="ON430_000229"
     CDS             35689..35955
                     /gene="rpsQ"
                     /locus_tag="ON430_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR3218179.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36013..36381
                     /gene="rplN"
                     /locus_tag="ON430_000230"
     CDS             36013..36381
                     /gene="rplN"
                     /locus_tag="ON430_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR3218180.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36418..36726
                     /gene="rplX"
                     /locus_tag="ON430_000231"
     CDS             36418..36726
                     /gene="rplX"
                     /locus_tag="ON430_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR3218181.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36754..37293
                     /gene="rplE"
                     /locus_tag="ON430_000232"
     CDS             36754..37293
                     /gene="rplE"
                     /locus_tag="ON430_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR3218182.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37312..37497
                     /locus_tag="ON430_000233"
     CDS             37312..37497
                     /locus_tag="ON430_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCR3218183.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37533..37931
                     /gene="rpsH"
                     /locus_tag="ON430_000234"
     CDS             37533..37931
                     /gene="rpsH"
                     /locus_tag="ON430_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR3218184.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37963..38499
                     /gene="rplF"
                     /locus_tag="ON430_000235"
     CDS             37963..38499
                     /gene="rplF"
                     /locus_tag="ON430_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR3218185.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38660..39016
                     /gene="rplR"
                     /locus_tag="ON430_000236"
     CDS             38660..39016
                     /gene="rplR"
                     /locus_tag="ON430_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR3218186.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39037..39537
                     /gene="rpsE"
                     /locus_tag="ON430_000237"
     CDS             39037..39537
                     /gene="rpsE"
                     /locus_tag="ON430_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR3218187.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39552..39731
                     /gene="rpmD"
                     /locus_tag="ON430_000238"
     CDS             39552..39731
                     /gene="rpmD"
                     /locus_tag="ON430_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR3218188.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39775..40215
                     /gene="rplO"
                     /locus_tag="ON430_000239"
     CDS             39775..40215
                     /gene="rplO"
                     /locus_tag="ON430_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR3218189.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40215..41510
                     /gene="secY"
                     /locus_tag="ON430_000240"
     CDS             40215..41510
                     /gene="secY"
                     /locus_tag="ON430_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR3218190.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41572..42219
                     /locus_tag="ON430_000241"
     CDS             41572..42219
                     /locus_tag="ON430_000241"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR3218191.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42410..42628
                     /gene="infA"
                     /locus_tag="ON430_000242"
     CDS             42410..42628
                     /gene="infA"
                     /locus_tag="ON430_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR3218192.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42661..42777
                     /gene="rpmJ"
                     /locus_tag="ON430_000243"
     CDS             42661..42777
                     /gene="rpmJ"
                     /locus_tag="ON430_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR3218193.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42795..43160
                     /gene="rpsM"
                     /locus_tag="ON430_000244"
     CDS             42795..43160
                     /gene="rpsM"
                     /locus_tag="ON430_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR3218194.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43188..43577
                     /gene="rpsK"
                     /locus_tag="ON430_000245"
     CDS             43188..43577
                     /gene="rpsK"
                     /locus_tag="ON430_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR3218195.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43657..44595
                     /locus_tag="ON430_000246"
     CDS             43657..44595
                     /locus_tag="ON430_000246"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR3218196.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44623..45003
                     /gene="rplQ"
                     /locus_tag="ON430_000247"
     CDS             44623..45003
                     /gene="rplQ"
                     /locus_tag="ON430_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR3218197.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45275..46114
                     /locus_tag="ON430_000248"
     CDS             45275..46114
                     /locus_tag="ON430_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3218198.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46090..46959
                     /locus_tag="ON430_000249"
     CDS             46090..46959
                     /locus_tag="ON430_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3218199.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46949..47746
                     /locus_tag="ON430_000250"
     CDS             46949..47746
                     /locus_tag="ON430_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR3218200.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47949..48695
                     /gene="truA"
                     /locus_tag="ON430_000251"
     CDS             47949..48695
                     /gene="truA"
                     /locus_tag="ON430_000251"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR3218201.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPKERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49028..51697)
                     /locus_tag="ON430_000252"
     CDS             complement(49028..51697)
                     /locus_tag="ON430_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCR3218202.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        16407 a         9498 c        11263 g        14579 t
ORIGIN      
        1 gtttttaata gatttaataa gtactgataa tgaaatttct cctggttggg aaaacgaagt
       61 tggttaccct gtagaagaat tttttcaaga ttactttgcg gagtatgccg ataggataat
      121 gagagattac gtaacagata aaactcaaga agtcatcaat caatcccagt cattaaatga
      181 attatctgat aaggcgaaag aagaagcagt tgccattatt tcttcaggga ttagtgacta
      241 tattgatatg agtggtgaaa tcaattatgg cgataaagac aaaattgtac gcatgataaa
      301 agcttttatc aaaacttttg atgtgaaggc agatcttaag tttaatgata cttacctagt
      361 aggaaaatta attaatatcc ttgggacaga gaaagatacg gaaacgttta tttcctcttt
      421 gtctatgttg tttacagata agcatttttc aactcagctt ggtggagagg aactagtgga
      481 aatcttttaa ccattacgcc cagcattaat aaaagtatat caaaaaagta ctcctgatga
      541 tttttaagat tggtttgaga aataatgtga aaacactatt tttaatgtta attatagata
      601 gagatgaacg cgtcatatgg ttaaaagaat gaaaaagtca aagatacact gctatatttc
      661 actaaggata tagcagtgta tctttcatgt cgtttcactg gcagtttttt ataaaaggag
      721 gtgtgcattt cagttgcttt tctttggtag ttagttgaat cgtttaagaa ttaacggatg
      781 tggcagacag gtgctctgtc caaaaaccag taaaatcttt gatggttcca tctttattta
      841 gctgtgcctg ttcaaatact gttttgtttt taccgaatgg atcaattgca ataattgttc
      901 cgatccccgc acgtttggca gcaagtaatc ctgagtaagc atcttcgata acaagacaat
      961 cagccggagc taagctcagt ttgtctgccg ctttaagaaa aatatccggt tgcggtttcc
     1021 cagggaaaga gccgtcgtca tagacaatct tgtcccagtc aaaccagcgg gctaaatcga
     1081 aaatatcaaa ataaaaagca acattctctt tgacagtagc cgtggcaatc gttaatggaa
     1141 tctgttggtc agtcagctgg tcaagagtcg cttcaagccc ttttgtcaac tgaagctcgt
     1201 tttcattttg caagcaaagc atacgataat agctttcttt ttcttctgac aatacggcga
     1261 tctcagaggc ggtcaaatgc tctgaaatga aatgtttcaa aatctccgta ttcgtccgac
     1321 catgaatatt ttttaaaata tcctcgtctg ataaaccatc ttttgtatga tcatgtatca
     1381 tatgcagcca agcagcttca tgcagatgag agtccaaaaa cattgttccg ttgaaatcaa
     1441 atataatccc tttcatagtt acgctcctat agtggtattt tcggttgaat cagcaatttg
     1501 cagatcgaca tattgtgcat agtgctcttg aacctcccgc gagatctggc gatccgtaat
     1561 gagcagatcg aaatcatgca aattataaaa cacataaaaa tcatctcgat tgaatttttt
     1621 attatcaacg agtagatatt tcttacggga attatttaga gcgatctgct gtgcttctcc
     1681 ttcttcggta ctgtaagtag agatcgcttc gttgtgaacg ccatttgaac tgataaatgc
     1741 ttttgtaaac tttagtcttc ttaaattgtc gttggtgatc ggtccgacaa atgcgccggt
     1801 atttttccgg aaatcaccgc caatcagtag aatttctggt ccatctgctt gagcaagaat
     1861 atcaaaaact ggataattat ttgtaatgat ccgaattttc ttgttcagta aatgcgtcgc
     1921 caatagttcg attgttgtac ctggtcctaa gaaaatcgtt tctccctcgt gaatcaagga
     1981 ggctgctgtg gcagcaattt cttttttttc ttgaatctgc agagtggctt tttcagtgtg
     2041 agaaagttct tgatctaaag tgtaggaaag actttgagcc cctccatgga cacgcagcag
     2101 cttgcccgat ttttctaatt catctaaatc tctgcgaacg gtcatatcag aaacatctaa
     2161 ttgattgatg atttcattaa cggtaaggat tcctttttta ttgaccattt ctacgatagt
     2221 aagcaatctt tcttttttca acattgtctt cacctctaat ttaaggataa tctatcatat
     2281 aaacggacaa cagtcaatct aattcgaaca aaagtaaaca tttagggtgg tttttatgtt
     2341 tgttacaata gattttattg ctgttttctg gtttttatga ttatttgtgt ttgtttttgt
     2401 tgacaaatgt tttggatgcg tttacaatgc aatcagttag aaagagcgaa aactgattat
     2461 agatttgagg agagatgtac atgcttaaat taccagaaga ttttattttt ggcggagcca
     2521 cagcagccta tcaggttgaa ggagccacta aggaaggtgg caaaggcgct gttgcttggg
     2581 atgacttttt agaggaacaa gggcgattca gtcccgatcc ggcaagtgat ttttatcatc
     2641 aatatgcaaa ggacattgaa ttgtgtgaac gttttggtgt aaatggtcta agattatcca
     2701 ttgcttggag ccgcattttt cctgatggag ctgggaaacc aaatccagaa ggaatcgatt
     2761 tttatcatcg tgtgttcgaa gaatgtaaga aaagaaacgt aacgccgttt gtgacgttgc
     2821 atcattttga tacccctaaa cgtttgtttg accatggaga ttttttgaat cgagaaacaa
     2881 tagaagcatt tgtttcctat gccatttttt gttttcatga attcaaagaa gtcaaggtgt
     2941 ggagtacctt taatgaaatc tatccagttg caaccaatca atatttgtta ggtgttttcc
     3001 caccaggaat caaatatgat tttacaaaga tcgtcgcctg tcttcataat atgatggttg
     3061 ctcatgcccg tgtagtgaac tatttcaaag aaaacgaact tccgggtgaa attggtgtag
     3121 ttcactcatt agagacgaaa tatgcggcaa cagacgcgcc ggaagataag catgctgcct
     3181 ttttagacga tgcattgtcg atccgtttct tattagatgc tacctattta ggctattatt
     3241 caactgaaac cttgactgct cttgatgaga tatgtgaagc aaatcaggcc agctatcatt
     3301 ttcctgaaga agatttcgtt gaattgaaaa aagcatccac gcgtaatgac tacttaggga
     3361 tcaatcacta ccaatgccat tttgtcaaag cttacgatgg agaaaatgca atccatcata
     3421 acggtacggg tgaaaaaggg acctctgttt ataaagtgaa gggaatcgga gaacgtattt
     3481 ataaagaagg aattcctcga actgattggg actggctgat ttatccagaa ggtctatatg
     3541 acttgttgct gcgaattaaa agcgattacc cacattacaa caaaatctat atcactgaga
     3601 acgggatggg gtataaagat cagtttgaag atggcattat tatggatcag ccgagaatcg
     3661 actatttgag agtctatttg gaatccttaa gcaaggcgat aacagccggg gtaaatgtga
     3721 aggggtattt tttgtggtcg cttatggatc ttttctcatg gacaaatggg tataacaaaa
     3781 ggtatggctt gttttatgtt gattttgaaa cccagaagcg ttacccaaaa gaatcagctt
     3841 actggtacaa gttggtcagt gaaaccaaaa cgattattta ataagtgata atggaggaag
     3901 aaaagatgaa taaagaggaa ttacaaatgc ttggttttga gatcgttgca tattcaggcg
     3961 acgctcgcag tacattattg aaattattga aagaagtaag acaaggaaac tttgaacatg
     4021 ttgattcggc gttgaaagaa gccgacgaaa atttaaccct tgcacataat tcgcaaacaa
     4081 aaattttagc ggaagaagca tctgggaaag agatggagat gggattcatt tttatccatg
     4141 ggcaggatca tttaatgacc actttattgc tgagagatct gattcaagac tttattgtac
     4201 tgtacagaca gaatcaagga taggagaaga acgctatgga tggaatcatt aaacaaatcg
     4261 aaaaagggaa accatttttt gaaaaaatct cacgtaatag ttatttagga gccgttcgtg
     4321 acggattttt aacagcgatg ccagctattt tattttcaag cattttcatt ttgattgctg
     4381 cggttcctga gatttttggt taccaatggc ctgaatctgt ctccacatgg ctatggaaag
     4441 tctataatta ttcgatgggt gttgtagggt tactcgtttc agcaaccact gctcgctgct
     4501 tggctgattc gatgaatcgt cgcttaccga aaaacaaagt aatcaacacc acctctgtga
     4561 tgctggcctc tgtggtaagt ttcctgttgt taagcgtctc tccgattgaa ggtggatttt
     4621 caacggaata tatggggaca aagggactac tggcttcctt tgtcgctgca tttttaacag
     4681 ttaatttata taagttttgt gtgctgaaag atattaccat taaaatgcct aaagaagttc
     4741 ctggaacgat ttcgcaaacc ttccgagata tttttccttt ctctttttca gtatttgcgg
     4801 ccgttttaat tgatttaggc attcgacaaa tcttccaaat ctcatttgca gaagcaatca
     4861 ttacggtgct gcagccgcta tttacagcag ctgacgggta cttaggaatc gctattatct
     4921 ggggagcgat ggcgctgttt tggtttgtcg gtgttcatgg accgtcgatc gtggagccgg
     4981 caattgcggc aattatctat gccaatgtgg aaaccaatct gcaattgttc aattccggtg
     5041 aacaagcttc taaagtactg acggtgggtt tagggaattt tgtaggaact atgggaggaa
     5101 ctggggcaac attggttgtt ccttttatct tcctactctt tgctaagtct aagcagttga
     5161 aggcagttgg gaaagcttcc tttattccag taagtttcgc tgtcaacgaa ccattgctat
     5221 ttgcggcacc catgattttg aacccttatt tttttattcc atttttgtta acacctgttg
     5281 taaacgtttg cctgttcaag ttttttgtcg atgtattggg catgaacagc tttatgtacg
     5341 ttttgccttg ggcaactcca gtaccgatcg gtttggttct aggaacgggc atgggtatct
     5401 tagcttttgt tctagcagcg gttctaatcg tcgttgactt cctgatctat ctaccatttt
     5461 gcaaagcata cgatgctgtc ttagtagaga aagaagcaca aaaagcagag gtgatcgcaa
     5521 ccgaggaagg tgcttctgcc gatgtacgtg aggaagtacc tgtggcagcg agtcaagtta
     5581 tcagcgatgc aaataaagaa atcaatgtgt tggtactttg tgctggcgca gggacaagtg
     5641 cgatgttagc taatgcttta aaaaaaggtg catctgaatc gaaagcaccg atcagtgcaa
     5701 tggcgggtgc ttatggttct cattacgaaa tgctgcagga ttttgatatg gtggtcttag
     5761 caccgcaagt caattcttac tatgatgata tcaaagaaga caccgatcgc ttaggaatca
     5821 agctggttgc gacaaaagga gcagaatata tcggattaac gagagatcct gaaaaagctg
     5881 ttcagtttat tctgaaccaa tttcaataaa ggaggagcca taatgaaaat gggaatttgg
     5941 ttgcgtaaag ctgttttatt tgcagctgtg ttaggggctt tgggggctgt ttcaaaaact
     6001 gttgaagcat ctgccagcga atttattcgc ggagcagata tttcgatcct gtcagatatg
     6061 gaaaaatctg gtgcaaaata ttatgaagac gatatttcaa aagatgcctt agagatttta
     6121 aaaaataatg gtgtcaacta tgttcgactg cgtttgtggc aggatcctta taatagtaat
     6181 ggagaaagtt atggagcagg gacaaatgat ctggaaacga cgattgacct tgcaaaacga
     6241 gcaaaaaatc tgggacttaa ggtgctgctg gattttcact acagtgattt ttgggttgat
     6301 cctgggaaac agaatcttcc taaagcatgg caaggtctga cctttgaaga aatgaatacg
     6361 gcattgtacg actatacaaa gaacgttctg tcggaaatga agcagctgga cgtttatcct
     6421 gatatggttc agatcggcaa tgaactgaat agcggaatgc tctggcctta tggcaagtct
     6481 tggggagaag gcgggggaga atttgatcga ttggctgctt ttttgaagtc tggtatccaa
     6541 gctgttcgag acactcaacc taaaacaact ccagtaatgc tccacttagc tgatggtggc
     6601 gatacaggag catttacttg gtggtttgat gaaatcacga gtcgaggtgt gtcatttgat
     6661 ttaattggag tgtcgtatta tccgtattgg cacggttcaa tgaatgaact tcaaaccaat
     6721 atgaatgcga tcagccaacg gtatggtaaa gaagtgatcg tagttgaaac agcttatggg
     6781 catacaacag cgaatgccga tactatgcca aatgcttttg gtgaagccga agcagctgcg
     6841 gggggatatg aaccaacacc gacaggacaa gcagagtatt tgctggatct tgccgatcgg
     6901 attcaggcag taccgaataa tcgcggggcg ggcttcttct attgggaacc tctttggtac
     6961 aacggaaatg tgagttgggc aacacaagca ggtatgaatt atctcagtgt tcaatccact
     7021 atgggaaacg aatgggacaa ccaagcgatg tttgattttg ctggaaacgc cttgccaagc
     7081 ttgcgtgcgt ttaaacaagc aggggcgcaa ataaatctag ttaaaaatgc aagtttcgaa
     7141 agcgatggac caacgaccag tccttccaat tggcaaacct ggtttcaaca aggaaccgct
     7201 aagggtacga tcaaaacgga agcgggcact ttttttgggg actacaaact aactttttgg
     7261 caggatcaag catacgaggc atcagcttat caaaatgtta gtggcataag caaaggaact
     7321 tacacattga gtgcctatgt aatgtctggg ggagagtttg atactaatca gttatatatc
     7381 aaaaattatg gtggagcgga actaaatact gcggttgtta aaacaagtga atggacaaaa
     7441 attacaatca aagacattcc aattacaaca ggtgtttgtg aaattggggt ttatagcaag
     7501 gcttcaggaa atgcttggac gaacgttgat tttgtttcat tggtgaaaca atagactgcg
     7561 gatcgtttcg attcaagatc ggaggttccc ttcagccaca tcgttagcgt agaaggcagg
     7621 aatcgaacct gcacctcgcc tttgccaaaa ataaccagaa acgatttcca gtcattttta
     7681 ccaaaacgag taaagcacca gacttcatct acactttgta ttatgctaaa gagagttcaa
     7741 cgatttcgta agaagcaatg aaaaaattgc aaaaagttgt gcagataaca taaagtttgt
     7801 ttaagaaagg gtaaccttag aattccgtcg tgaatatgat ttgagcactt agatggatag
     7861 aaaaaagttc tcggaaaaca gttcgttttt cgagaacttc tttttatgat aagaatgaaa
     7921 caaattaact tatattatag tctcttttgg tagggaacca tcttattttt aatgaaaatc
     7981 aagcatttta gctattgaag aatgttgatt tatagggatt ccccctccat attatgttgg
     8041 ctctttattc ccactccact gttgctggtg gtttagaagt gatgtcatac acgatacggt
     8101 tgacgtgggc tacttcgttt acgatacgta cagagatttt ttgcaagact tcccatggga
     8161 tacgggcaaa gtcagcggtc attccgtcga ttgaagttac agcacggata ccgactgtgt
     8221 agtcgtaagt acgtccgtca cccataacac cgacagagcg gatccctgga agaacagtga
     8281 aatattgcca gatatcacgg tcaagtccag cagctgcgat ttcttcacgc aagatagcat
     8341 cagattcgcg tacgatttct agcttgtctt cagtgatttc gccaagaaca cgaataccaa
     8401 gaccaggacc agggaatggc tgacgccaaa cgattgaatc aggcatgcct aactgagtcc
     8461 ctaattcacg gacttcgtct ttgaataatg tgtttaatgg ttcgatcaat tcaaattgca
     8521 tatcttctgg cagtccacct acattatggt gagatttgat cgtctgtgct gtttctgtac
     8581 cagattcgat aacgtcagta tacaacgttc cttgtgctag gaaagagacg ccttcttcac
     8641 cagcaagttt tgttgcttcg tcatcaaaaa catatacgaa ttcattccca atgattttac
     8701 gtttttgttc tgggtcagat acacctttca acttgtctaa gaagcgatct cttgcatcga
     8761 ctttgatgat gttcaagccg aattttccgc ctagactttc cattacttgt tccgcttcgc
     8821 ctttgcgtag cagaccgtgg tcaacaaaga tagatgtcaa ttggtcaccg atcgcttttt
     8881 gtaaaagaac cccaacgaca ctagaatcaa cgccacctga aagaccaagc aagacttttt
     8941 tgtcaccaac ttgttcacgg attttttcta cttgcatatc gataaagtta tccattgtcc
     9001 agttaccttc acagtgacaa acatcaaagg caaagtggcg cagcaattcg attccgtatt
     9061 cggaatgacg tacttctgca tggaattgga taccataaaa attacgtgtt gtgtcttgga
     9121 tagaagcaat cggacaatct ttgcttgttc cgactgtttc aaagccttca ggaactttag
     9181 ttaccaagtc accatggctc atccatactg tttgttgttt tggtgtttct ttaaacaaga
     9241 cagcttgatc gcttgttact tctaacaagg ctttcccata ttcgcggttt ttagcaggct
     9301 ctacggaacc gccaagtttg tacgtgatca attgcatacc atagcaaatc cctaatacag
     9361 gaatacctaa attgaagatc tcaggatcga tcccaaatgc attctcatca tatacactgt
     9421 ttggtccgcc agaaagaatg attccctttg gattcatcgc ttttacttct tctgcagtaa
     9481 tgcggtggct caataattcg gaaaagactc caagatcacg aatacgtctt gtgatcaatt
     9541 ggttatactg actaccgtag tctaatacga taatcttttc aacgttcgtc atatcggcaa
     9601 cgtttgtcaa atttattacc cctatccctt taaattttat ctcaaaatag tcaaactatt
     9661 ttaaaatgct aatattttcg catgaaaatc gaatcgatga tatgattttc agttttatgg
     9721 agaattatat cggctcttcc tcgtgtaggc aagatatatt cctgtaaatt tttcaaattc
     9781 acatttttcc atacatcttt tgccatttta aaggcttctt cacgatctcc gatagcaaaa
     9841 cgataataat aattctctgg attttggaaa gccgtatcca gtaacgcacc aaaacgatcc
     9901 aaataccatt tttcgatcag cacgggatcg gcatcgacat agacagaaaa atcaaaaaag
     9961 tcgctgacgt agatttgctg attcgcaggt aattgaagcg tattgatccc ctcaacgatc
    10021 aagatatcag gctgctggat caattcgtac ttgtctggaa tgatatcata cacatcatgt
    10081 gagtaaacag gtacttttat ctcttcctga ccagatttta cacggttcaa gaaatcgatc
    10141 agcatctcca tatcataact ttcaggaaat cctttacggt ccatgattcc acgctctttt
    10201 aatatacgat ttggataaag aaatccgtca gtcgtgatca gctgaacgtt tcttcgtttg
    10261 aaggtacgag acaaaatcgt ctgcagcagt ctggctgttg tactttttcc aacggctaca
    10321 cttccggcga tcccgataat aaaaggcggt acttctacgt atttatgaag aaataaacct
    10381 ttactcaaga acaaagattc aaattctttc atataaagat aaatcagatg agcaagaggt
    10441 acatagatat ctcgaacatc ctgcatggaa atgcggtcat tcaaactttt gatattatct
    10501 agttcatctt gtgtcaaagg tgcttgccca ttacgataaa attctcgcca ttcttctctt
    10561 gccacacggt agtagttcat tggatcattc atattagcct cctgcgttct cccatcaatg
    10621 gaactatttt aacacaaaat aaacgcaaac cccaccactt aagagagatt gccgatcttt
    10681 ttgtataatc tttataatag aaagaaacgg agtcttattc ttgacttgcg cacggataca
    10741 taaaaggata gactgttgtc atgaaagaaa tgagaaggat ggaagacatg acaaatcatt
    10801 actattctga acgcccagat acggcgcacg attttgaaca atgggctttt gagttaaaag
    10861 gcagaacctt tcaatttgta acagattctg gtgtattttc acgtgaaaca gtggattatg
    10921 gttcacgtgt cctgattgat gctttcgatt gggaagagtt acctgatgaa ggaaagatct
    10981 tagatgtggg atgcgggtat ggaccgattg ggttagcttt ggcatttgcg acacagcggt
    11041 ttgttgaaat ggtcgacatt aatgaacgag cggtaagtct agcgcaagga aacgcaaagc
    11101 gtaaccaaat cgaacatgtt gatatccatc aatcgaatat ctatgaagca gttcatgaag
    11161 aaacctatgc agctatcgta agcaatccgc cgatccgtgc tggaaaaaaa gttgtacatg
    11221 aaattttgac aggcgcatat cctcggttga agaagggcgg tacactgaca attgtgatcc
    11281 agaagaaaca aggagcgccg agtgcgcaaa agaaaatgga agaaacattt ggtaatgcag
    11341 aaatcgtcac aaaagataaa ggatattata tcatcaaaag tgtgaaagaa taactagctg
    11401 aaaaatagtg gagcgacgaa agagtggaac tgggcgtgca tgtacgggta cgtatcagtt
    11461 ctttttttgt tgagaagttt ttaaaaacaa agaactattt tttcttcaaa aagaaatagt
    11521 aggaacagag tcgttgtttt ttacgataaa ataaataaga tacagaactt gtatagaagg
    11581 ctgccaagtc ggtagataaa ggaggttttt atcaaaaaac atactttcct tttttctcta
    11641 ttcttttctt gggggttttg gtacactgat agggaattta gatagaaggg agccaatagg
    11701 atgaagctga ccatcagaga tatcgcagaa atggcaggcg tatcagtaac aactgtctca
    11761 cagattttga ataacaaagg aagcagattt agtgataaga caagaaaaaa agtgttgtcg
    11821 atcgtgaatg aacatcatta taagcccgat tatttcgctt caaatgtgat caatcgacat
    11881 tcaaagacca tcggaatgat cgtccctgat gtcactgatt tcttcttctc caaactgatc
    11941 gaaggtgtag agagctattt gaatcctttg ggctatgtga tcatgttatg caattctcgt
    12001 catagccaag aaaatgagat caagtattta caagagcttt cccaccgctc agtggacgga
    12061 atcttgctgg ccacaccaaa tattttacct gagaaatatg cattagacag cggtttttat
    12121 caaaaaatgc caatcatttt aatcgatcgc ggattgaatc gtcgggataa cggacggctt
    12181 atcgtaaagg aatatgaggg cgcctatcaa gctgtttcgc tgtttattga aaatggccat
    12241 acgaagatcg gcatgctgaa ggaaaccact ggatactatc aattagaaga acgtttcaac
    12301 ggatatcgtc atgcgctgaa agatcatggc ctgcctttta atgggaaatt tgttgaacat
    12361 ggagagttga ctgtccaagg tggatatact gcatcaaaaa aattactcaa acataaagat
    12421 atcacggcta ttttttgcgg gaatgatgcc atggcgatcg gatgctatca ggcgatcgat
    12481 gaattgggca aaaaaatccc agaagatatc tcagtcatag gattcgatgg attaaagctg
    12541 tcagaataca tgattccgca attgacgaca gtgcagcagc caagttttga tattggattt
    12601 tatgctgcac gtttcctgat cgatacgatc gaatttcctc aaagaaaagt tccgaacaaa
    12661 gtgttcgaaa caaaattaat tattcgtgag agcgtaaaag tattgacaaa gggctaactc
    12721 ggcggtatat tgcttatgta agcatttaca aaggctagtt agtgcttttt ttatttttag
    12781 taaaccagtt tactaaagtg gtttaccttt cctaaaaata atgtataata ccgaatgaag
    12841 acaaggagga tcaggcaatg cgaatggtgg atctgattga aaaaaagagg gatggaaacg
    12901 agctgtcgaa agaggaaatc gagtacatcg tcacgaacta tacgaatgga aagattccag
    12961 attatcaagt cagtgcatta ttgatggcaa ttttttatca agacatgaca aatgaagaaa
    13021 tcacgaactt aacattggcg attgctaatt ctggtgatgt catcgatttg agttctcttg
    13081 aaggaataaa ggtagataag cattcgacag gtggcgttgg tgacacgacc acattgattt
    13141 tagcccccct agtagctagt gtcggagtga cagtggctaa aatgtctgga cgcggtctag
    13201 gttacacagg tggtacacta gacaagctag aagcaattcc cggatttcag atcgagcttt
    13261 cggatgaaga atttgtacgc attgttaatg aaagtaaagt agcggtaatt ggtcaatcag
    13321 gaaacttggc accggcggac aaaaaattgt atgccttgcg tgacgtcaca gcgaccgttg
    13381 attctcttcc gctgattgcc agttcgatta tgagtaaaaa aatcgcggca ggagcagatg
    13441 caatcgtttt ggatgtgacc actggagacg gtgctttcat gaagaacatc gaagatgcaa
    13501 gacgtctggc aaagaccatg acgagcattg ggaaactggc taaccgagaa acagtggcgg
    13561 ttatctcaga tatgtcagaa cctcttggcg aagctatcgg aaatagtcta gaagttgtcg
    13621 aggcaatcga aacattacaa ggaaatggtc cagaagatct agtagaaatg tgctacgcct
    13681 taggaagcca aatggttgtt cttgctggca aagctaagac aaccgatgaa gcaagaacgt
    13741 tgcttcaaga agctctggaa tcaggtaaag cgttagcaaa attcaaagaa atgatccaaa
    13801 atcaaggggg agacccagcg atcgtggaac atccagaacg aattttgact gcacgctaca
    13861 caatggagct tcctgctaaa caaagcggag ttgtgtcaaa aatcgtggca aatgaattag
    13921 gaattgctgc aatgatgcta ggtgctgggc gtaaaaccaa agaagaagat attgatcacg
    13981 ctgttggatt gaaactgcat aaaaaaattg gtgacactgt gacaaaaggt gagtcactat
    14041 tgacgatcta tagtaacgac gaagaaatca cttctgtcat tgaattatta tataaaaata
    14101 ttgaaattgg tgaatcggca atgaagccaa cgttgattca tgacatcatc acagaataaa
    14161 ggaaaaaagc ggactttacc gttttaaaga aaaggggaaa agaaatggaa ctaaaccgaa
    14221 tgatcgacca tactattttg aaagcagacg catctaaaga agctgtgatg caaattatcg
    14281 aagaagcaaa aaaataccat ttttattctg tctgcatcaa tccagcatgg gttgcgctag
    14341 cgaaagaaca attacaaggt gagccagtag ccgtatgtac agtaatcggg tttcctctag
    14401 gagcaaacac acccgagaca aaagcatttg aaacaacaga tgccatcaat aacggtgcag
    14461 atgaagtcga tatggtcatc aatatcggtg ctttgaaatc aaaggatgat caacaagtcc
    14521 aaaaagatat cgaagcagtt gttgaagcag caaaagatcg tgcgcttgtc aaagttatta
    14581 ttgaaacatc gttattagat agagaagaaa tcatccgagc ttgcgaaata gcaaaagctg
    14641 caggcgctga ttttgtcaaa acatcaactg gtttctcaac aggcggagca aaggtagagg
    14701 atgtccgttt gatgcgtgaa actgtaggac cagaaatggg tgtcaaagct tccggcggta
    14761 tccataacga agaagaagca atggcaatga tcgaagctgg tgctacaaga atcggaacaa
    14821 gcgcaggagt agcaatcgta tctggttcaa ctggtgaagg ctattaattt tttcaaagga
    14881 gaagctcatg gcagaactca aacaagaatg gatcaacaca gctattgaag cgttagataa
    14941 agcctatgtt ccttattccc attttccggt aggcgcttgt ctcgtaacgg aatcaggcaa
    15001 gatttatcaa ggcatcaaca ttgagaatgc atcatttgga ttaacgaatt gtgccgagcg
    15061 cacagctttt ttcaaagcag tatctgaagg agaaaaaaca tttacgcatc tagtcgtagc
    15121 ggggcacacc cccgatccta tctctccttg tggtgcatgt cgacaagtaa tggcagaatt
    15181 ctgttcccct gatatgccgg ttacgctagt aggagacaat ggtgtgacga aggcaacgac
    15241 cgttcgtgca ctgctgcctt atgcatttac tgaaaaagat ctataactca tgttcattca
    15301 gtcagctgac tgttgaaaaa taaaacaatt attttaggag gctttacaga aatgaaaaaa
    15361 gcaaaattat ttggtttagg tgctgtcgca ctagcagcag ggttattttt aggtgcatgc
    15421 ggcaacaacg gctctactga ttcatccggt ggcaaaagct catcagacac aacaactgca
    15481 gcattgatca cagacacagg tggtgtagac gaccgttcat tcaaccagtc agcttgggaa
    15541 ggactagaaa aatgggggaa agatcaagga ctttcacgag gaaatgatgg cttccaatat
    15601 ttccaatctt caaatgaatc agattacatc ccaaatattg accaagcatt gaatgctgga
    15661 ttcaaaacga tttttggtat tggttataaa ctaaaaccag cgatccaaga acaagcaaca
    15721 aacaatacag gtacgaactt tgttattatc gatgatgtca tcgatggatt agataacgtt
    15781 gtatctgcaa ctttcaaaga caacgaagct tcttatcttg caggtgttgc agcagcttat
    15841 acgactgaaa caaacgtagt cggctttatc ggcggtgtaa aaggtgaagt aatcgatcgt
    15901 tttgatgctg gattcaaagc cggtgttgat gcaggtgcga aagaattagg aaaagaaatc
    15961 aaagtattga atcaatatgc tggtgacttc tctgctccag ataaaggacg ttcaattgct
    16021 caaggtatgt atgcacaaaa cgcagatatc atcttccatg cttcaggcgg tacaggtaac
    16081 ggtgtattcc aagaagcaaa atcattgaac gaatcaggcg acaaaaaagt ttgggttatc
    16141 ggtgttgacc gcgaccaatc agacgaaggc gaatacacat taaacggcga aaagaaaaac
    16201 ttcacattga catctacact gaaagcagtt ggaacagtag tagaagattt ggcacaaaaa
    16261 tcagcggacg gtaaattccc tggcggcgaa cacactgttt atggattgaa agaagacggt
    16321 gttggtctaa cagaaggaca actttctgac gaagcgaaaa aagcagttga tgaagcaaaa
    16381 gaaaaaatca tttcaggtga cgtaaaagtt ccagaaactc ctgaagaaaa ctaaaaccat
    16441 ttctatagcc ttgttagaca catgtttgac aaggctatgg tttgattaga acaagaattg
    16501 ttcatttatc caagctgcct tgtgacagtt tggataaggg aacaactccc tgaagtaatg
    16561 tttgtttcaa aagaagaacg ctcactaagc gtgaatctta gtcaatcgtt gccctagata
    16621 gaaatcgtta ggatgtgaaa aaaaggtggt tcaagaaaat attgtcatag aaatgcagaa
    16681 tattaccaaa gaatttggaa cattcaaggc aaatgacaac atcaatttac aagtaaaggc
    16741 aggagaaatt catgcgttgc ttggagaaaa cggtgctggc aaatctacat tgatgaacgt
    16801 gctttccgga ttattagagc cgacatcagg gaaaattttg atgcgtggga aagaagtaaa
    16861 gatcacaagt ccgaccaaag ccaaccaatt agggattggg atggtccatc agcactttat
    16921 gcttgttgat gcctttactg taacagaaaa catcgtgttg ggaagcgagc ctagtcgcgc
    16981 agggatgctt gaccataaaa aagcacgaaa agagatccaa aaagtttctg aacagtatgg
    17041 attgtcagtc aacccagatg cttacgttcg tgatatttca gttggaatgg aacaacgggt
    17101 agaaatttta aaaacacttt accgaggagc agatgtactg atttttgatg agccgacagc
    17161 tgtattgacc cctcaagaaa ttgatgaatt aatcgtgatc atgaaggaat tagtcaaaga
    17221 aggcaagtcg atcattttga ttacgcataa gttagatgaa atcaaagcag tagctgaccg
    17281 ctgtacagtt atccgccgtg gaaaaggaat cggtacagtc aacgttaaag acgtaacctc
    17341 acaacaatta gccgatatga tggttggaag aacggtttca ttcaaaacga tgaaaaaaga
    17401 agcgaagcct caagaagtcg ttttgtctat tgaaaatcta gtggtgaaag aaaatcgtgg
    17461 attagaagcc gtgaaaaact tgaacttaga ggttcgtgct ggcgaagtac ttggtatcgc
    17521 tggaatcgat ggaaacgggc agtcggagtt gatccaagct ttgactggtt tgcgaaaggc
    17581 agaaagcgga catatcaagc taaaagggga agacatcact aataaaaaac cccgaaaaat
    17641 cactgaacat ggtgtaggac acgtgccaga agaccgtcat aaatacgggt tagtcctaga
    17701 tatgacatta tctgaaaata tcgcactaca aacctatcat caaaaacctt acagtaaaaa
    17761 cggcatgctg aattattcag taatgaatga acatgccaga gaattgatcg aagaatatga
    17821 tgttcgaaca acgaatgaac ttgttcccgc aaaagcttta tcaggcggaa atcagcaaaa
    17881 agcaattatc gctcgtgaag tcgaccgaga tcctgatctg ttgattgttg caaatccaac
    17941 tcgtggactg gatgtaggtg cgatcgaatt tattcataaa cgtctgatcg aacaaaggga
    18001 caaatacaaa gcagtgttac tgatcagttt cgaattagaa gaaattttaa atgtttcgga
    18061 tcgtattgct gttatccatg aaggagaaat tgtcggtatc gttgatccga aagaaacatc
    18121 tgaaaatgaa ttaggtctat tgatggcggg ctatacctta gaagaagcac gaaaagagtt
    18181 agaaaagggg gctggcgcac atgaatgatc gcagcgaaaa attccgtaat ttattagtgc
    18241 ctgtactttc agttgtttta ggattgatct taggcgcgat attgatggct gcttttggtt
    18301 ttcagcccat ccaaggttat tcttctatgc tagatgcagc ccttggttcg caaagaggga
    18361 taggtgaaac gttaaggcaa gcaacgccgt tgatttttac agcattaggt ttttctgtag
    18421 ctaactcagc tgggtttttc aatattgggc tttctggaca agcactttgc gggtggatcg
    18481 tcagtgtctg ggtggcactt gcgtttccag atctgccaaa agttgttttg cttccgttat
    18541 gcgtgatctt aggtgcctta gctggtgcga tggcagcggc tgttccaggt ttacttcgag
    18601 cattttttgg aacaagtgaa gtgatcgtaa caatcatgat gaactatatc ttattatacg
    18661 taagcacatt tgctttacat gaagtgatgc ctgaaagcta tcgctcaagt ttggactctt
    18721 ccaatatgat cagtcaaaat gcctcactgc ggttaccatg gttaacacaa gcttttggcg
    18781 gctctcgcgt aaactcagga ttatttttag caatcatcgc gcttattttc gtttggctgc
    18841 tgatgaagaa aacgacttta ggatttgaaa tccgttctgt tggattgaat ccttttgctt
    18901 ctgagtatgc aggaatgagt agtaaacgta cgatcgtttt gtcgatggtc atttctggcg
    18961 ctttggcagg gcttggcggt gtggttgaag gattaggaac gtatcaaaac ttctttgttc
    19021 aaacgacttc gctatctatt ggatttgacg gaatggctgt ttccttgctt ggttctggat
    19081 cagcaatcgg tattctttta tcagcgatct tgttcagtat tttgaagatc ggtggacttg
    19141 gcatgcagac aggagcaggt gttccgtttg agatcgtcaa tgtatcgatt gctttgatta
    19201 tcttctttgt cgggatcaac ttcctgattc gtttcgtcat ggctaaagtc ttccaaggga
    19261 aaaaacaaga agagatcgtt gcgacaatag aatcgaaacc aacgaatcaa aaccatgaag
    19321 ggggcgaact ataatgtcga ctatcgaatt aattgccttg atccttactt ctacactggt
    19381 ttattcaaca ccgctaattc taacgtcact aggcggaact ttttctgaac gaagcgggat
    19441 cgtaaacgta gggctagagg gaatcatggt catgggtgct tttagttcca tcgttttcaa
    19501 cttgacgatg agtccatcac ttggttctat gacaccatgg atcgctattt tagtgggtgg
    19561 cgtcgtaggt atcctatttt ctctgattca cgcagtagca acagtcagtt tgcgtgctga
    19621 ccatattatc agcgggacag taattaatct gatggcacct gctttaggtg ttttcttgat
    19681 caaagcatgg tatggtaaag gtcaaactga taacatcacg cagaatctgg ggtatttttc
    19741 tttcccagga ttagcagata ttccggtcat tggaccaatt ttctttaaaa atacattctt
    19801 acctgcttat tgtgctattt tgattgctgt cctagcttgg gtgatcatct tcaagacgaa
    19861 attcggcttg cgcctccgtt cagttggtga aaatcctcaa gctgccgaca cattaggtat
    19921 caatgtatat gctatgcgtt attcaggcgt actgatttct ggcttgttag gcggtatggg
    19981 cggtgctgtt tttgctcagt cgatttccgg taacttttca gctggtacga tcgttggaca
    20041 agggttcatc tccatggctg ctatgatctt tggtaagtgg aatcctttag gggcaatggg
    20101 tgcctcattg ttctttggtt ttgctcaaag cttgagtatc attggcgcac aattaccagt
    20161 tatttcttcg attccgccag tattgctgca aattgctcca tatttgctga caatcattgt
    20221 gctggttgtt ttcttaggga aagcttctgg tccaaaagca aacgggaaga actatatcaa
    20281 atcaaaataa tcttttagcg aataaaaagg gtgggttacg ggaagtgttt ttcgtctcgc
    20341 cctttttgtt ttaaaggcaa taaaaataaa aagaaagaag gaataatcat gtttaaacgc
    20401 gtacatctga tcgtcatgga ttcagtagga attggagaag caccagatgc cgataagttc
    20461 ggtgatgttg gttctgatac attaggacac attgcgaaag aagcaggatt gaacgttcca
    20521 catttagagc agcttggttt aggaacgatc cgtcctcttg aaggtgtgga atcagtgaaa
    20581 gatcatcaag gctatgcgac gaaattagaa gaagtttctg tagggaaaga tacgatgaca
    20641 ggccactggg aaatcatggg attgaatatc aaaactccat ttcgtgtgtt tcctaatgga
    20701 ttccctgatg atctgatcaa acaaatcgaa gaattttctg gccgtaaagt cgtatgtaac
    20761 aaaccttata gcggaacagc tgttattgac gattacggtg aacaccaaat gaaaacaggc
    20821 gatttaattg tatatacttc tgccgatcct gtccttcaga tcgcggcaca tgaagaaatc
    20881 attcctttag aagaattata tcggatttgc caattcgttc gtgatataac aaaagatgat
    20941 ccgtatatga ttggccgaat cattgcacgt ccgtacgtgg gtgaaccagg agacttcaca
    21001 cgaacaagca atcgccatga ttatgcgttg aatccattcg gacacacagt ccttgattct
    21061 ttgaaagaag caggaaagga cgtgattgct gttggaaaga tcaacgatat tttcaacgga
    21121 caaggaatca ctgaatctgt ccgcacgaaa agcaatatgg atggcgttga ccagctgctg
    21181 aaagttatga aaaacgattt ttcaggaatc agctttacca atttagtaga ttttgatgca
    21241 ttatacggtc atcgtcggga tgtagtcggc tatgcgcatg cgattgaaga atttgactta
    21301 cgaattccag aattgcttga agcgatggaa gaagacgact tgctaatgat tacagccgat
    21361 cacggaaacg atccgacgtt tactggtacc gatcatacgc gagaatatgt accgttattg
    21421 gtttacagta aaaaaatgaa agaacaaggg gcattgcctc aaggatatta ttcagatatt
    21481 tcagcaacga ttgctgagaa ctttagtgtt tcttcgactg aaaatggcga aagcttcttg
    21541 aaattattaa aataatcttt ttaaatagga gaaagcaaat gaccaagtta agcgagatgt
    21601 tacaagaaac gacccagttt atccttgata aaggagtaaa agaaattgat tttggtttga
    21661 ttctaggttc aggattaggt gaattagcag atgaaatcga agatgctgta atgatttctt
    21721 atgatgagat cccgttcttt cctgtttcga cagtagttgg gcatgcagga aagctagttt
    21781 atgggtctct ggcaggcaag aaagtgttag ccatgcaagg gagattccac tactatgaag
    21841 gtcattccat gcaaacagtg acctatccag tacgtgtgat ggccggacta ggggcccatt
    21901 cagtcattgt gactaatgca tgtggtggtg tcaacgaatc atttgtacca ggagatttga
    21961 tgctgattac cgatcatatc aattttactg gtgaaaatcc tttgatcggt ccgaatgaag
    22021 atgcaatggg ccctagattt ccagatatga gtcaagctta tacgcctgac tatcaagcaa
    22081 cagcaaaagc agcagcagaa aaactaggtt tggatcttaa agaaggcgta tatatgggct
    22141 attctggacc aacttatgaa acgcctgcgg agatccgaat gtctcggaca ataggagccg
    22201 atgcagtagg gatgtcgact gttccagaag tcatcgtagc agctcacagc ggattaaaag
    22261 tgttaggaat ctcttgtatt accaacttag cagcaggtat gcaagcgaat ctgagccatg
    22321 aagaagttgt agaaacaact cagagagtca aacaatcttt taaagcattg gttaaagaaa
    22381 cattgacatt attgtaatag gaggaacaat aaaatgagtg tgcatattga agcgaaagaa
    22441 ggcgagatcg ccgataagat tttacttccg ggagatcctt tgcgagctaa atatattgcg
    22501 gagacttttt tggaagatcc agtttgttac aatcaggtac gcggtatgtt aggctttacc
    22561 ggcaaatata aaggagaacg tatttctgtt caaggaacag gaatgggaat gccatcagcc
    22621 accatctatg cccatgaatt gatccagtcg tatggtgtaa agaaattgat tcgagtaggt
    22681 acatgcggtg cattgtcaaa agacgtccat gttcgtgatt tagtactagc acaaggtgca
    22741 gcgaccagtt cttcgatgat cgaaaaaaat ttccaagcct ttcattttcc tccaatcagt
    22801 gatttcaatc tattactgaa agcatatgag atagctaaag aaaagggcta tacggttcat
    22861 gtaggaaatg ttctttcgga agattctttc tacaaagatg atttgacaga gacgttccaa
    22921 ttagctgaac ttggcgtttt aggagtggaa atggaagcag cggctcttta ttacttaggc
    22981 gcgaaatacc atgtgcagac attaagtttg atgaccgtta gcgaccattt gatcaccggt
    23041 gaagaaacaa cagctgcaga acgtcaatct acgttcaatg aaatgatcga agtaggattg
    23101 gaaacagcga tagcagaata agaaggaaat gaaaaagctg ccctgcatta agtaataaga
    23161 acatccaagc aaaaatagct cctcatgttt ttgacttggc tggacttatt attgcaaatg
    23221 taagcttttg aacaccgttt accaagaggt tgtgaatctg ccgtttaact ctgagtcaca
    23281 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgaga
    23341 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
    23401 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
    23461 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
    23521 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
    23581 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
    23641 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
    23701 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
    23761 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
    23821 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
    23881 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
    23941 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
    24001 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
    24061 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
    24121 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
    24181 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
    24241 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
    24301 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
    24361 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
    24421 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
    24481 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
    24541 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
    24601 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
    24661 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
    24721 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
    24781 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
    24841 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
    24901 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
    24961 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
    25021 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
    25081 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
    25141 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
    25201 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggaacc
    25261 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
    25321 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
    25381 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
    25441 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
    25501 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
    25561 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
    25621 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
    25681 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
    25741 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
    25801 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
    25861 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
    25921 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
    25981 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
    26041 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
    26101 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
    26161 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
    26221 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
    26281 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
    26341 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
    26401 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
    26461 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
    26521 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
    26581 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
    26641 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
    26701 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
    26761 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
    26821 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
    26881 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
    26941 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
    27001 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
    27061 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
    27121 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
    27181 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
    27241 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
    27301 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
    27361 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
    27421 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
    27481 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
    27541 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
    27601 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
    27661 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
    27721 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
    27781 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
    27841 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
    27901 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
    27961 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
    28021 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
    28081 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
    28141 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
    28201 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
    28261 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
    28321 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
    28381 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
    28441 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
    28501 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
    28561 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
    28621 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
    28681 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
    28741 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
    28801 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
    28861 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
    28921 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
    28981 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
    29041 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
    29101 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
    29161 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
    29221 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
    29281 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
    29341 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
    29401 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
    29461 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
    29521 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
    29581 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
    29641 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
    29701 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
    29761 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
    29821 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
    29881 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
    29941 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
    30001 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
    30061 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
    30121 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
    30181 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
    30241 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
    30301 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
    30361 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
    30421 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
    30481 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
    30541 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
    30601 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
    30661 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
    30721 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
    30781 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
    30841 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
    30901 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
    30961 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
    31021 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
    31081 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
    31141 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
    31201 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
    31261 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
    31321 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
    31381 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
    31441 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
    31501 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
    31561 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
    31621 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
    31681 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
    31741 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
    31801 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
    31861 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
    31921 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
    31981 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
    32041 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
    32101 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
    32161 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
    32221 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
    32281 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
    32341 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
    32401 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
    32461 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
    32521 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
    32581 ccatggatga caagaaatac acttttgaag tggacactcg tgcaaacaaa actttagtaa
    32641 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
    32701 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
    32761 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
    32821 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
    32881 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
    32941 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
    33001 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
    33061 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
    33121 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
    33181 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
    33241 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
    33301 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    33361 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    33421 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    33481 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    33541 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    33601 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    33661 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    33721 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    33781 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    33841 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    33901 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    33961 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    34021 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    34081 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    34141 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    34201 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    34261 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    34321 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    34381 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    34441 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    34501 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    34561 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    34621 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    34681 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    34741 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    34801 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    34861 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    34921 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    34981 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    35041 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    35101 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    35161 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    35221 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    35281 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    35341 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    35401 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    35461 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    35521 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    35581 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    35641 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    35701 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    35761 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    35821 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    35881 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    35941 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    36001 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    36061 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    36121 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    36181 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    36241 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    36301 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    36361 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    36421 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    36481 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    36541 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    36601 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    36661 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    36721 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    36781 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    36841 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    36901 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    36961 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    37021 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    37081 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    37141 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    37201 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    37261 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    37321 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    37381 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    37441 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    37501 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    37561 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    37621 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    37681 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    37741 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    37801 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    37861 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    37921 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    37981 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    38041 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    38101 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    38161 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    38221 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    38281 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    38341 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    38401 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    38461 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    38521 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    38581 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    38641 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    38701 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    38761 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    38821 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    38881 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    38941 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    39001 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    39061 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    39121 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    39181 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    39241 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    39301 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    39361 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    39421 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    39481 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    39541 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    39601 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    39661 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    39721 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    39781 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    39841 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    39901 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    39961 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    40021 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    40081 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    40141 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    40201 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    40261 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    40321 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    40381 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    40441 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    40501 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    40561 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    40621 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    40681 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    40741 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    40801 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    40861 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    40921 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    40981 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    41041 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    41101 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    41161 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    41221 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    41281 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    41341 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    41401 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    41461 agttagaagg cttaatgctg aaacgtaaat atgttggatt tattgagtaa gaaagcgtgt
    41521 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    41581 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    41641 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    41701 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    41761 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    41821 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    41881 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    41941 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    42001 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    42061 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    42121 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    42181 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    42241 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    42301 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    42361 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    42421 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    42481 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    42541 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    42601 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    42661 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    42721 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    42781 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    42841 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    42901 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    42961 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    43021 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    43081 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    43141 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    43201 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    43261 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    43321 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    43381 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    43441 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    43501 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    43561 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    43621 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    43681 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    43741 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    43801 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    43861 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    43921 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    43981 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    44041 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    44101 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    44161 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    44221 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    44281 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    44341 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    44401 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    44461 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    44521 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    44581 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    44641 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    44701 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    44761 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    44821 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    44881 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    44941 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    45001 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    45061 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    45121 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    45181 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    45241 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    45301 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    45361 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    45421 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    45481 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    45541 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    45601 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    45661 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    45721 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    45781 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    45841 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    45901 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    45961 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    46021 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    46081 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    46141 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    46201 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    46261 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    46321 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    46381 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    46441 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    46501 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    46561 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    46621 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    46681 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    46741 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    46801 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    46861 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    46921 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    46981 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    47041 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    47101 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    47161 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    47221 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    47281 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    47341 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    47401 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    47461 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    47521 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    47581 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    47641 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    47701 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    47761 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    47821 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    47881 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    47941 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    48001 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    48061 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    48121 cgatcgggca agtaatccat tttgattatc caaaggagcg tccgttagaa cgaatgcgtt
    48181 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    48241 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    48301 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    48361 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    48421 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    48481 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    48541 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    48601 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    48661 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    48721 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    48781 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    48841 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    48901 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    48961 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    49021 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    49081 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    49141 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    49201 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    49261 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    49321 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    49381 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    49441 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    49501 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    49561 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    49621 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    49681 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    49741 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    49801 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    49861 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    49921 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    49981 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    50041 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    50101 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    50161 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    50221 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    50281 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    50341 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    50401 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    50461 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    50521 gagcagctac gaccaaaatg attcctaaca acagcccacg atcgtcttgt tctatctttt
    50581 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    50641 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    50701 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    50761 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    50821 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    50881 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    50941 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    51001 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    51061 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    51121 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    51181 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    51241 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    51301 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    51361 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    51421 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    51481 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    51541 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    51601 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    51661 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    51721 tgaagttgta ttcagtttat catatcc
//
LOCUS       DAJSMI010000005        51032 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJSMI010000005 DAJSMI010000000
VERSION     DAJSMI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.005"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            590..748
                     /locus_tag="ON430_000253"
     CDS             590..748
                     /locus_tag="ON430_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3218203.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            820..2046
                     /locus_tag="ON430_000254"
     CDS             820..2046
                     /locus_tag="ON430_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3218204.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2135..2527)
                     /gene="rpsI"
                     /locus_tag="ON430_000255"
     CDS             complement(2135..2527)
                     /gene="rpsI"
                     /locus_tag="ON430_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR3218205.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2543..2986)
                     /gene="rplM"
                     /locus_tag="ON430_000256"
     CDS             complement(2543..2986)
                     /gene="rplM"
                     /locus_tag="ON430_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR3218206.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3505..3972)
                     /locus_tag="ON430_000257"
     CDS             complement(3505..3972)
                     /locus_tag="ON430_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCR3218207.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4092..5630)
                     /locus_tag="ON430_000258"
     CDS             complement(4092..5630)
                     /locus_tag="ON430_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR3218208.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5710..6396
                     /locus_tag="ON430_000259"
     CDS             5710..6396
                     /locus_tag="ON430_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR3218209.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6597..7124)
                     /gene="msrA"
                     /locus_tag="ON430_000260"
     CDS             complement(6597..7124)
                     /gene="msrA"
                     /locus_tag="ON430_000260"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3218210.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7208..7885)
                     /gene="pgmB"
                     /locus_tag="ON430_000261"
     CDS             complement(7208..7885)
                     /gene="pgmB"
                     /locus_tag="ON430_000261"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR3218211.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7882..9012)
                     /locus_tag="ON430_000262"
     CDS             complement(7882..9012)
                     /locus_tag="ON430_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3218212.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9012..11195)
                     /locus_tag="ON430_000263"
     CDS             complement(9012..11195)
                     /locus_tag="ON430_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR3218213.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11192..12217)
                     /locus_tag="ON430_000264"
     CDS             complement(11192..12217)
                     /locus_tag="ON430_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3218214.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12262..13089)
                     /locus_tag="ON430_000265"
     CDS             complement(12262..13089)
                     /locus_tag="ON430_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3218215.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13086..14153)
                     /locus_tag="ON430_000266"
     CDS             complement(13086..14153)
                     /locus_tag="ON430_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR3218216.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14179..15015)
                     /locus_tag="ON430_000267"
     CDS             complement(14179..15015)
                     /locus_tag="ON430_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3218217.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15012..15881)
                     /locus_tag="ON430_000268"
     CDS             complement(15012..15881)
                     /locus_tag="ON430_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3218218.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15951..17234)
                     /locus_tag="ON430_000269"
     CDS             complement(15951..17234)
                     /locus_tag="ON430_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR3218219.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17248..18933)
                     /locus_tag="ON430_000270"
     CDS             complement(17248..18933)
                     /locus_tag="ON430_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR3218220.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19208..20233
                     /locus_tag="ON430_000271"
     CDS             19208..20233
                     /locus_tag="ON430_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3218221.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20828..24502)
                     /gene="rpoC"
                     /locus_tag="ON430_000272"
     CDS             complement(20828..24502)
                     /gene="rpoC"
                     /locus_tag="ON430_000272"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR3218222.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(24560..28177)
                     /gene="rpoB"
                     /locus_tag="ON430_000273"
     CDS             complement(24560..28177)
                     /gene="rpoB"
                     /locus_tag="ON430_000273"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR3218223.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28905..29873
                     /locus_tag="ON430_000274"
     CDS             28905..29873
                     /locus_tag="ON430_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3218224.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29891..30796
                     /locus_tag="ON430_000275"
     CDS             29891..30796
                     /locus_tag="ON430_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218225.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30793..31599
                     /locus_tag="ON430_000276"
     CDS             30793..31599
                     /locus_tag="ON430_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3218226.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31735..32724
                     /locus_tag="ON430_000277"
     CDS             31735..32724
                     /locus_tag="ON430_000277"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCR3218227.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33041..33565)
                     /locus_tag="ON430_000278"
     CDS             complement(33041..33565)
                     /locus_tag="ON430_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCR3218228.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33852..35549
                     /gene="scm"
                     /locus_tag="ON430_000279"
     CDS             33852..35549
                     /gene="scm"
                     /locus_tag="ON430_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR3218229.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(35704..36690)
                     /locus_tag="ON430_000280"
     CDS             complement(35704..36690)
                     /locus_tag="ON430_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR3218230.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(36713..37279)
                     /locus_tag="ON430_000281"
     CDS             complement(36713..37279)
                     /locus_tag="ON430_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCR3218231.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(37293..37709)
                     /locus_tag="ON430_000282"
     CDS             complement(37293..37709)
                     /locus_tag="ON430_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR3218232.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(38166..40100)
                     /locus_tag="ON430_000283"
     CDS             complement(38166..40100)
                     /locus_tag="ON430_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3218233.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            40381..41337
                     /locus_tag="ON430_000284"
     CDS             40381..41337
                     /locus_tag="ON430_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCR3218234.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            41427..42530
                     /locus_tag="ON430_000285"
     CDS             41427..42530
                     /locus_tag="ON430_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR3218235.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESTLKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            42543..43496
                     /gene="menA"
                     /locus_tag="ON430_000286"
     CDS             42543..43496
                     /gene="menA"
                     /locus_tag="ON430_000286"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR3218236.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(44310..45656)
                     /gene="gor"
                     /locus_tag="ON430_000287"
     CDS             complement(44310..45656)
                     /gene="gor"
                     /locus_tag="ON430_000287"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR3218237.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(45790..47043)
                     /locus_tag="ON430_000288"
     CDS             complement(45790..47043)
                     /locus_tag="ON430_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR3218238.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(47049..47972)
                     /locus_tag="ON430_000289"
     CDS             complement(47049..47972)
                     /locus_tag="ON430_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR3218239.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(48193..50685)
                     /locus_tag="ON430_000290"
     CDS             complement(48193..50685)
                     /locus_tag="ON430_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR3218240.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(50692..>51032)
                     /locus_tag="ON430_000291"
     CDS             complement(50692..>51032)
                     /locus_tag="ON430_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCR3218241.1"
                     /translation="YVINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQ
                     LFDDTISEKNAFAIIQKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAF
                     LERLSYEDGK"
BASE COUNT        14743 a        10783 c         9408 g        16098 t
ORIGIN      
        1 gttgctgaag attagaatga caaaactatt tgaaactacg cgtatagtaa gtagctttca
       61 aaatatataa aaaaggagcg ttctcaaatg agaacatgct cctaaaacta ctttattggc
      121 gtatggaaaa attatactta aagtggatag cgggttgaat aagttgataa gcatcaaaac
      181 atttataaaa attattcaag actatgtctg ttccatatac aaatatgaaa atcagtaaac
      241 tccatttaac ttttttcgta gtgtgtcttt ttatacatgt ttatcgttat taatagtgaa
      301 taggaaaaaa cctatttttg atcattgaca attaaataca atttttgaat aacgatccct
      361 gctattcgag ctagaaactc aagtacgcca tgacctgatt agcgacaaat acttagagat
      421 ttctttttgt tctgggaaaa caaaatgcca tgacaatagc agcaaataat gatacttccg
      481 taaggctcgc cgtaaactgg ggagaggtga aattcctatg tgcttaccca aagcaattca
      541 aaaattctaa tcttttttag gagatgattt tataaaattg agagtctact tgaataaaaa
      601 acaaacctgt gagtttctag gaattgataa taatacttta gataagtgga ttcgtatata
      661 taattttcca gtaatcagaa ttgacggtat agttcgcttt gacatagagg atatcaaaga
      721 ttttatgaat acatttaaaa caatttgaaa atttcttctc gccgcatata ctaagaatag
      781 cgaaatatac tgccttactt tttaagggag tgaggaattt tgcctacaaa actttcaaat
      841 gggaaatata aaactaatct gaggtatcca aaaaaattca gagaaatcac tggaattaca
      901 tcagaaaagt atcaaaaagt atttccaact aggcaattgg ctattaaagc cgaaaatgca
      961 atgaaaagga aaattgaaac agttttaaga gaggagaatg caaattcact agaattaaaa
     1021 ggaaaaataa aatttaagga attctatgaa tcaaaatggc ttcctagata tgaactaggt
     1081 cagactacta gatcaaaaag agctccaagt tacgtaacaa ttagtaatac taaagatata
     1141 ttccgattgc acatccttcc aatgtttggt gagtatgcaa tgaactatct aaattcaaat
     1201 actgagataa tatcagatga attaacaaaa aaatcgaaag aatacgcaaa tataaaaatt
     1261 atcaaaggat atgtccgttc tatgtttgat attgcagaaa tactaaatta tattgaattt
     1321 aaccgaacta ccaaaattat tcaatgtatc actgcaccta aaaaaattgc tttagaagaa
     1381 agaaggatac gggaaggtaa tcaagcatta tcgtcagaag agcttattgc ttggatagaa
     1441 gcagtaaaca ctgacttgaa taatcacttg cttactcttc atgactatac tcttttcatg
     1501 cttaccctat atcttggtga tagaaaaagt gagacgtatg ctttgcaatg gaaatatatc
     1561 gactttgaga aacaaacggt tcgccttaaa catgctttgg ataaatatca aaaaaagaaa
     1621 tttacaaaag gtcgaaagga taccgtaatt caagtacccg aaattgtaat ggcattacta
     1681 agcaaatgga aatcagtgca agctgagcaa ttgttgagat taaaaattaa tcaaacacca
     1741 gatcagtatc tctttaccta tactaaacca tccggagaag taaattgccc tgttcacgct
     1801 gattacctaa actatcgaat caattcaatt aaaagacgac atcctgaatt ggctcattta
     1861 tctccacata agcttcgtca tacctatgca actatagcta gacaaggcgg agcaaatatg
     1921 aatcaaatat ctagtgcttt aacacactca gatatttcta caacgagaat atacgtaaat
     1981 acaccagatg tagtagataa aactgttttt gaagcctttc agagaggatt gaataaatgt
     2041 gattagtatg tgttcttaaa tgtgttctta aacaagaaaa agacactttt caaacaattg
     2101 aaaagtgcct tgaaacattg atatataaat aatattaacg tttagagaat tgagatgctt
     2161 tacgagcctt tttgagacct ggttttttac gttcgaccat acgtgcgtca cgagtaagta
     2221 agccagcacg tttaagagct aaacggaagt caggatcaac ttctagcaat gcacgagcga
     2281 taccatgacg gatagctcct gattgtccag cgtatcctcc gccgtttacg ttaacaaata
     2341 cgtcatatgc gccttttgtt tcagtaacag cgaaaggttg attgattact tcacgcaagt
     2401 cagcatgtgg aatgtattct tcaacatctt ttttatttac agtaattttg ccagttcctg
     2461 gtactagacg tacgcgagca actgcgtttt tacgacggcc tgtgccgata tattgaactt
     2521 gtgccaatga aattccctcc tattagatta agtttgtgat gtctaaaact tctggttgtt
     2581 gtgctgcatg tggatgttct gctccaccgt atacgttcaa ttttgtaaat tgtttgcggc
     2641 ctaaagtgtt tttaggaagc atacctttta cagaagtttc gatcaaacga cgagaatttt
     2701 tagcacgcaa ttcgccagca gtgattgatt tcaatcctcc tgggtagttg ctgtgacggt
     2761 aataaatttt gtcagtcgct tttttacctg ttaatttcac tttgtctgca ttgatgacga
     2821 tgacgaaatc gccagtatcc aaatgaggtg tgaaagttgg tttattttta ccacgtagta
     2881 cagatgcaac aactgttgaa agacgtccca atggaacatc agttgcgtct actacatacc
     2941 atttacgttc tacttcgcct ggtttggcca tatatgttgt acgcacgtta ttttcctcca
     3001 atttaagttc tgatgtttga catacacttc gagtttccgg ggctctttgt ggggcaaaca
     3061 ataccattta acatattacc cgctaatcta gtcaatgtca acgtttttct gcttgattga
     3121 aaaatttata tgaaacttta cttcttttct ttcttttata taaaagggtc gtttcctgtc
     3181 aaaatgaaaa acaaaaagag gctgtgacaa aagtcttgtc acaacccctt attttgaata
     3241 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatccataaa acataagaag
     3301 ctgtttttta aaattgtttc ttgtgactcg gagctgaacg gcaggcttac aacctcttgg
     3361 taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa aatatgagga
     3421 actattttga cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt
     3481 ttcggtcaag ttcaacaaca gtttttacgc gtcgatctct ggcgctttgc ccaaatgagc
     3541 ttggatcatc cagatacgtt tttctgtggc tgttttgaat tcgataaaga tatcttgtgt
     3601 tggatcgtcg ccttcttctc cagaaacttc gattcctttt tggtaaagat ctgctagata
     3661 acgatagcct gcaacaagtg tttccaaacg ttctggaata gtacgatccc agcggccaat
     3721 ttcatcaggg atttttgtgt tatctgcaaa ttcacgcaat gtagaataag gtgaaccgtc
     3781 taatgtgatc aagcgttcgg atacttcatc taactgagcg ttcaaatcat ccatgaactc
     3841 atccatcatt gggtgcaatg taaggaaacc tggtcctctc atgtaccaat gtgtttggtg
     3901 aacaacaaca gacatttggc ttagatctgc aactaattga tttaatactt ctttggtttg
     3961 ttggaatttc atctttcttg cctcctaaca ttttcttaca cttaaaataa tagtagactt
     4021 tcaatgaaaa gtacaacaaa atcatttacc tgagaacgat tttcagttcg cttttttatc
     4081 cgtaaagcca atcatttttc agaatcagct aattcttctc gcagttgagc aaattgctga
     4141 taagtctgtg ctaaatgctt ttgtgtcgaa acaaataatg gatagatttt ctgatatttt
     4201 ttatgatcag tttcttgtac tccaacttgc tctttcaatt ctatcaattc ctgtgcttca
     4261 ctgatacttt ggatctttcc gatgcttctc cagcctaaaa tcacagctcc taatgcggat
     4321 gcttctgtga cagtagggaa aacaagcggg taacccagta catcagccat catctgacga
     4381 aaaatcggag attgagcaaa cccgcctgta gcacggatct ctgtgagctg attttcctgg
     4441 caaactcctt ttaaaatccg ataaaggttg agacagatcc cttctagaat cgccttcatc
     4501 atttcttttt ctgtatgttg ccgctgtaaa ccgataaaac tgccaaacgc atctgcgtcc
     4561 caaagcggtg ctcgctcacc aagtaaatag ggatgaaaaa tcagaccatt ggcacccgct
     4621 ggcacaaaag cgatagccga aagcagtgca tcatatggat tttctccttt ttctttagca
     4681 gattgaacga cttcctgtaa aaatgtatgc cctgcccaat caaagatgcc agcaccattg
     4741 ctagtcgctc ctcccactac ccaatgtttc tgatccagaa cataacaaaa cgtctccatt
     4801 tctggatgga actgaggctg agtagtaata aaacgaagtg ctccgctcgt tcctacggtc
     4861 aaagcggcga tcgtatcacc aaaagcacct aaccctagat tagaaagcgg cccgtctgcc
     4921 cctcccaaaa caaatgatat atcatttggc aaacctagtt ctgctgctgt ttttgatggt
     4981 agacctgtta cttgataggt ggaaggaaag acttgcggca attgctctat tcgaatgttc
     5041 aagtagtcaa gcacttcttc tgcccattgg aattcatgga tatcaaaata tccagttcct
     5101 gaagctgttc cgtagtcgct gatcagttcg ccaaacaatc tataaaagat atagtctttg
     5161 ataccgacaa aataagcagt ttttttgaat aattcaggtt ggctgttttg cagccacttg
     5221 attttgttca atggtgtcat tggatgaatc ggcgtcccag tctgccgata aaattctttg
     5281 ccaagcacgg tatatttcag tttttctgct tcgtccgctg ctcggttatc tgcccaagtg
     5341 atcattcgag ataacggtgt tttctcttca tccaacaata tcaggccatg catcgcagag
     5401 gagaaggaga caaattgaat gattgtgtct gaaggcaact gtttaattac ttcttttata
     5461 acggtgcaga caccttgaaa gatcaattcc tgatcttgtt ctgccatccc ttgtgtttct
     5521 tggatcaaag ggtagcgaaa ataagcggat gctgcttctt gggcttcatc tgtgaaagca
     5581 actgctttgg tttgtgtggt tccgatatct aatccgattg aaacgttcat tttttcacct
     5641 caaattttat gtatgtacaa aaatttagtt taagcttgcg tatcttggtt ttgaaaaggg
     5701 ggagatggcg tgttacttta ttttatcatt ggttgctgta tcggatcatt tttatgttta
     5761 accgcccagc ggattccgtt aggtcactct attatttatc ctagatctca ttgtgtcaaa
     5821 tgctgtcgtt cgctttcttg gtatgaattg attccgattc tctcgatcat cgtccaacga
     5881 tttcgctgcc gctattgcag gtgtcggttg cccttctact atttgttggc agaagcctta
     5941 tgtggtggtc tttttgcctg gttctttact ttttcttccg caagaaattt ttcgacgttc
     6001 atatggatgc tttcggcttt gctattttcg ttgatggatt tattttactt catggtccat
     6061 tctcacacgc tattctgttc ttgggcaatt ttatggattt tttggttgca ggccggcgtc
     6121 ttccaatggc aaagtgtgct gttaacgttt atcgtaggcg gtgtgtgcct tcgctatggt
     6181 caatcctatc tcggagctgg cgatactctg ttgatgcttt cttggagtgg tggcttgtct
     6241 ctagaagagt tgcttcagat cttgtttctt gctagtctgt ttggaatatc cgttttttct
     6301 gtttacttta tctgtcataa aaaaaagttg gaaaagctgc ctttcattcc ttttttgagc
     6361 agtgcgttgc tgattgtcct tcatcttagg tattgaaaaa aggttgtgac aaacgacttg
     6421 tcacaacctc tgatatatag tgttctctta ccaaatcctt gtgacttgga gctaggcgac
     6481 aggttcacaa cctctccact aaatggtgtt caatcagctg accctcagta ttagctcaaa
     6541 atgctggttt tacaacctct gatatacggt gttccataag tagctttttc ggcttcttat
     6601 ttcttgacgc cccaatgtcg acgaaggaaa cctgcacgtc cagaaccttc tcgataaaga
     6661 ttgtaatgga gcttgttttt cttgtaataa tcctgatggt aatcttctgc aggataaaat
     6721 tcgcttcttg gttcgatttt tgtcacgatt ggcttatcga atcgcccgct gtcttgcaat
     6781 gcttgttttg aacgctctgc aatatttcgt tcttcttcat cattatagta gatcactgga
     6841 cgataagaat caccgcgatc agcgaactgt cctaatgcgt cagtcggatc ggtttgctgc
     6901 caatagattt ccaccaactg atcataagaa ataacttctg gatcatattc gatctctact
     6961 gcttctgtat gccctgttgt gcctgttgtt acttgttcat aagttggatt tggtacatgc
     7021 ccgccagtat aaccggatgt cacagaaatg atccctggct gctcttcaaa tggtttgacc
     7081 atacaccaaa agcatccgcc tgcgaaaact gctttttttg tcatataaaa tccccctttt
     7141 ttctcatcat accttgattt ccaaaaggag gataacgttt tgcttgtgac agaaaaaacg
     7201 gaaactatta tatacgtgat acggcttctg ataaatgcca aaacgaacgt acttgatcaa
     7261 aaacggttaa atcaggaatc acatattcag cagaaggaag atttatgttt tcacctattc
     7321 ccaatacatg catgttacca gccaaagctg cttgacaacc agcttctgaa tcttcaatga
     7381 ccaagcatgc atttggcggt acattcaatt gctgtgctcc tttgagaaaa acttctggat
     7441 caggttttgc ttttgatact tgcgtgccgt caataaggac atcaaaataa cttgtcaaat
     7501 tcgttttttc taaaatgagg cgtgcatttt tgcttgctga tcccaatccg atcttgatgt
     7561 ttgttttttt caagtactca agaacagcta ttgcaccagg caaaacggat gtttcatcca
     7621 ttttttgaat cgcttgaaga tatagattgt ttttttgctc agcaaaggct gatttctctt
     7681 tttctgagaa tttctgttcc atttttccaa atgacagcaa tcgctctagt gactcggttc
     7741 tggatattcc tttcagttgc tcgttttctt tctcagtgaa tggaatggac agctggttag
     7801 ctaaaaccag ccacgcttcg taatgataac gagctgtgtc tacaagaacg ccatctaaat
     7861 cgaatagtgc tgctttgatt ttcatcgatt acctccttga aaatgaacga cttgaagtaa
     7921 agatgcgatg tttttagctg tgcgatgaag attatcagtg gcttgctgta aagcttcttg
     7981 taaaggcaca gctgcaggaa ggatcggaaa aacggccgaa atgccaacat ctgctgcttt
     8041 ttcgcttcct ttaccaacac ttccgcaaat agctatcaca ggaacgccct ctggtactct
     8101 tctagcaatc ccaactggcg cttttccttt tagactttgt ccatctagac gtccttcgcc
     8161 tacgattacg aggtctgctt gctgtacccg tttatcgaac tccagtaaat ccaatacgta
     8221 gtcgatgcca gagactattt tccctgaagc aaatgctaac agtcctgcac ccattccacc
     8281 accagcacta ctgcccgatg cctcgattac ttgaggggca aatctcatat aaaaatcatg
     8341 catggcttgg tcagccactg ctaattcatt ttctttcaag cctttttgtg gtccgaaaac
     8401 aaaagaagcg ccttcttttc cgcataaaag attagtaaca tcagtaatga cagtgatttt
     8461 ggctttaatc agctcaatag gcacgttcgt tttatcaact gtagcaattt tacctagatt
     8521 ttctcccagt gcttctactt tttgattatc ttgatcgtaa aaagcataac ctagaccttc
     8581 tgccatgccg atgccgccat cgttggtact acttccgcca acgccgatga tgatgtgtcg
     8641 aacaccttgt tgcaacaatt gtcgtatcag ttctcctact cctcgcgtgg ataacctaag
     8701 tggctgttgt tggtttagtg ggacatcttt caatccgacg atttctgcca tttcaaaaat
     8761 cgcagtttga ccgtctgaag cgtactggat ttctttgttt ttgccaaatg cattagtaac
     8821 gactgctgtt tgccgcttca accttaaact gtctgctaat gatgaaaccg ttccttctcc
     8881 tccatcgcct ataggtagca gatcatattc agcctgaaca aaaacttctt gaaaacctga
     8941 ttgaatggct tgtgctgcct ctaaggctgt cagactttct ttgaaagaat ctggcgcaat
     9001 cagaatacgc attattttcc cctccgttcc aagatcagtt ctttttccag ctggtattgg
     9061 tctccattga caaaaatagg taatgtttct ttcgtctctt tttttattac tatttgtttt
     9121 tttgtcaaaa caaatagcaa cctgtgattt ttccaaataa aaggaaaaga aattttttcc
     9181 catgctgaag gaagtcttgg agaaatagaa agagttccat tcgttactgt aatacctgaa
     9241 aaaccaaata taactgctag ccatagtgct cctaatgaag cagcatgaat tccgtcatct
     9301 gatgaatgcg gatctggtcc taaatcaatc aggcacgctt cttggaataa atgatacgcc
     9361 cattccattt cttggatacg gcaagcctct atggcgtgaa tcgctttact caacgatgag
     9421 tcgtggattg ttttgttttc atagtaatgc aaattcttag aaacgattgg ttgagaaaaa
     9481 tactcaggta ataagtataa cagcatgaca agatctgctt gcttgctgac ttggagttca
     9541 tttacttcgc tacgggcata atcaagtaaa atagactggc tgccaggacg ttctttgtaa
     9601 gtggtcaaat caataactgg cttactgaga tacgtgtcat cttgcggaat cagctgttct
     9661 ttgttaggca ctggcaaata taacttatct aaaaaatttt gacattcttt caaaaaaagc
     9721 ggatcgccag cttgatattt aacagctaag gccacattat aatgcgccag atagttcgta
     9781 tatgcattat tgtcgatata ttctgtgtat tcatctggac caatgactct gtgtatctct
     9841 aaatgatcat cgacttttac agctctgctc agccaaaaag ctgctgtttc atgaatgagc
     9901 gtttttccct ttgtttctaa aaacgattga tcctctgttg cttggacata ctcttgaacg
     9961 gcataagcaa ggtctgcact gatatgatgt tctgcggcag ctgaagcaac ttcttgtctt
    10021 gttccggttt tgatattcaa tgcagcaaat ttgggtgtct cttcctgacc agtgtgtgcg
    10081 ctttcccatg ggaaaagtgc accttgatag tgataatctc ttgctttttg ccgtgcgcct
    10141 tctaatcgat cataacggta cagcagcaaa ttctttgcta cttcaggaaa ctgatataaa
    10201 aagaaaggca ggataaaaat ttccgtatcc cagaaaacat gccctttata accttcgcca
    10261 gttagtccct tcgcccctac actaacactt ggatcattgc ggggagtcat aatctgtaaa
    10321 tggtaagcag cgaagtccat cgctaactga tcgaaagaag aatcgccttg gatcgttatc
    10381 cgatgctgat gccaaaaatc tgtccaataa gacatcgact gctctaaaat ttctgtataa
    10441 ctaacgcttt tctgggcttg ggcaatactt ttcttaaacg ggtctgtttg ctgaagagag
    10501 gtagagagca cgatgatctt ctccagcgta aaggacgtat tttttctgat cttttctgaa
    10561 aaggaagcgg tgatccggcg attttttgct tggaatacac ctgctttatt gaatttagct
    10621 gcaatcccaa tatcaatttt actttctgtt gtctggtagg atgcactgat caaatcgtca
    10681 ccaaatactc gcagttcttt ttcaaccaac tgctgtgtac caaaattggt ttgctgtcca
    10741 tcgatacccg ttttcacatc tacttggcag tcttcggtca acggcttgat gaccactttc
    10801 agggctgcct gatggagatt attttgatca gcaacacgac aagtaatgac ttccactttt
    10861 tttccttgag cagtttcaat gagcgcatag cgtttaagct ctcccatttc taaatctagt
    10921 gcccgctcgt atgcaagaac tttcatcgtc tcaagagaaa aatgttcacc gtcgattttt
    10981 atttcccatc tgatgacatc tgggagattc actaactcgg ctgattctgc tcctaaaggt
    11041 cggttatata caccagctgc aaacattccc cttacttgat tccgatagtc ttcctcctgg
    11101 cttcctctga taccaaaaaa tccatttcca atggtcatct gactcgcata cttattgata
    11161 tactctaaat caaactgttt ttcttctact ttcataaggt aataacttct cctgtttctg
    11221 ccgaacgata taaattttcc accagattct ggatgacata tccttgccag ccatctgcaa
    11281 tcatctgagt ctggtcaccg agcgcccgat tcacaaaatg agccatactt agttcatgtg
    11341 ggtcattaac tggcgtggtc ttttgtaagg aaaccagctt tccatctaca tcttcgaata
    11401 gttcgcctgg atacagatta gcacctgcct gatcgccaca aaaagccaca ttcagtttgt
    11461 cttctctgat attcaaagca aacgaagtat tcagttggat gatcttgccg ttttctaatt
    11521 ccagtgtgcc aaaaattgca tcttctactg tgaatttgct tggatcccat ttcccgaatt
    11581 tccctgaatt ttttttcgta ccaatttttt ggaagctgta cgcccgaacg ctttttactt
    11641 taggaaatcc agtaatatac atcgctgtat caagcatgtg aatgccataa tcgatcaaag
    11701 gcccgcctcc ttgaagttct ttgtcgataa acgttcccca gcctggaaca ccagaccgac
    11761 gaagcgcatt cgcttctacg taataaatat ccccgagaac atcaagatga ttcttgatca
    11821 ttgttgcttc gtcagaaaaa cgatgctgga atccataagc caaaatctgc ccgtttttat
    11881 cggctgtctc tgccatcttt cttgcttgat cagctgtcat agctggtggt ttctcgcaca
    11941 tcactgaagc attagcttcg agggcagcca atacatgttc gaagtgaaag cgattgggtg
    12001 tacagacact cacgatatcg ggagaaatct cattcagcat ttgtgtaact gatgtatacg
    12061 catgagaaaa tcctgtacgt cggcaaaagt cttctgctcg ctggctgact ggatcaacca
    12121 cagcggccaa ttctaagaca tctgatcttt tttgataata agcagcatgt actttttctg
    12181 ctacttgtcc agcgcctatg attgctactt tcttcattta tttctccctc ctttgcttat
    12241 ttacttattg ttattctttc tctattcttt ctctaaatca tttaaatgac gcgctgtttc
    12301 cagacagttt ttcaagtatt gcaatgattc ctgataaatt ttcacttcat tttctcctcg
    12361 caaacgacat tcatatacca tataaccttc atactcattt tgaagtagcg tttgaaaatg
    12421 gcgagtaaaa tcaattgcgc cagatcctgg ttggtagcga tgattatctg ctaaatggat
    12481 atgacccaca tactcttttt gcgtagtcaa tgcttttgaa atatcatctt cttcaatatt
    12541 catatgataa aaatcagcga tgatctgcac atgtttcaat tgattagagt caatgtaatg
    12601 acgcgcatcc gctaacaaat tgatcatatg gtcttggtat cggttcaaag gctccaaata
    12661 aataaccgta cctgtttctt cggctacttt atctaaataa cgaagtgatt cgctgactgc
    12721 tttatagtct ccttctttgc ttcggggtga aaccatcggc ggtaatcgat aagtgaacat
    12781 tccccaagct gctgggataa cgattccttt gcctccaacc tcgcttagtg cacgcaaaat
    12841 ctcagcaatt tgacgtacac cattaagacg ccgttcttcg ataaagtcac cgatccaccc
    12901 atcgtagccg ttacaagctg tcactactgg gatccctgtt tctttgattg cctgtttgac
    12961 tgtgtcgata ttttctatta acaacttacc gtcgatttcg tattcttcaa atccacagtc
    13021 tttgataaat ctaaattttt ctaaaatgtc tcttgggaaa aatgcttgat cttgcgtacc
    13081 gaatttcatt gttctcctcc tcggacttga acgcccattt tgatactttc ttctgggtgc
    13141 tggtctacat aacgcatgta agcttctgcg ctttctgcaa atggcacaac tggatcaatg
    13201 atttcttcac aatccaagta accgttcatc aataattccc aacatgtgtc ctcgattcgt
    13261 ttacggttcc atcttgggta atctgggttt ggttcactag atgctcgagc aaagacgatt
    13321 tttgcattat tgaaatgtgc ttcgcgacca agattaaacc cagctggaaa aggtttagca
    13381 aaggcaacat aagaaatgat cccgccatag ccgatccctc ttaaagaagc ttgtaatgcg
    13441 gcagcactgc cactcgtttc aataactgaa tctactccac gtttatctgt caattccttt
    13501 agacgaaaac cgacatctgc tttcatagga tctagcgtgt aatcggcacc gtatttttct
    13561 gctaattctc tacgctgcgc tatgggatcg atcccaacga tggtagtagc acctgctttt
    13621 ttagctaatt gaatcgcaat ttgcccgata gcgcctaaac cgaagattgc tacagcatca
    13681 cctgctcgaa catgtgcttc ccggatacca cctaaagcga attgagcagg atcgtaacat
    13741 actgcatttt tccattgatc caaccgatca agtttacgta gtttataatt atcaacaccg
    13801 tttgtaatca gcgtttcacg aatgggtcca taagtgcaga ctaactctcc tttttgatac
    13861 tctgtcacca aactgcccgt ttcttcgatc gtaccgacaa tcatatttcc taattgaaat
    13921 tcgccaaata ccacaccgcg ttggcttcct tcttctcttg gcaagaatag gcgccattct
    13981 tctgaaaatt cttcatcgat aaatggactt aatcctctaa agtcgacaat ttctgttcct
    14041 ggttttggag tagcgaaaat tactcggata cgtacttcgt tcttttcgat ctcacggtct
    14101 tcgtaatctt tgaatccggc tattcttggt tctaatgcaa ctaatttttt catcttttct
    14161 ttcctctttc ttttcttttt atccttttac gccgccttcg actaaatcac ctttaataaa
    14221 tcgttcagat aacgcataca taatgatgac tggtaaagcg gtcaccaatg aagcggccat
    14281 cattcgtccc caaatgtagt ctggtgtact gaataatgtg ttcaaaccga ttggtaaagt
    14341 aaatttttcg gaggtcgaca ggaaaatcga agcaaataaa taatcattcc acgaaaccat
    14401 aaagcaataa acgaagacag aaatgattcc agaaatagcc aatggtataa tgatccgaat
    14461 aatgatctgg atccggctaa gaccatcgat cattgctgct tcttcaatat cagtcggaat
    14521 cgtgtcaaaa taacttttta gcatgaatat cgcagtagga agtgtttgaa cgaccatcgt
    14581 gataatcagt gctgtattgg tatcatacaa acctagaccg gaaatgattt taaataatgg
    14641 aacaacgatc aaaataccgg aaaacatata aacggtgtaa aagctagcat tgattttcga
    14701 gcgagctttg aagcgtagtt ttgacaaagc gtatgccccg ctgatcccaa tcactactgc
    14761 agttacggag gctatcaagg agatcactaa actatttttg aaatacatga tgaatggaaa
    14821 aatctcagca ttgaaaatat cctgataatg ttcaaaagtc cattctttcg gaaaaatagt
    14881 ttgtgtcgtt gcaattgctt cttgtgaatc cttgaatgat gtcatcagca tgatcaaaaa
    14941 tggaaacaaa ttgacagcta atagcaacaa aatcgtcgcg taaaataaga ttttcctgct
    15001 ctttttattt ttcatttcaa gtccaccttc tttcgtgaga gcaagacgac cgctgcaata
    15061 atgatgaaca atagaatcga tatagctgct gcttttccaa gatcattgaa cgcaaaggct
    15121 gtctgataca aataaacgcc taagatattg actttattcg tcagtagata gacatctgta
    15181 aacatataga acatccagat agatcgtaaa acaatcacag ttgctagtac tggtttgatt
    15241 gccggcagtg tcacgatctt gaacttctgc caagcatttg caccgtccat ttcagctgct
    15301 tcataaagcg ttttgtcgac tgtttgcaaa atagccaaaa atgaaataaa cgcatacgga
    15361 tagtatctcc agatagcaaa caaaacgacc ataaagaagc tagcaactgg ctggtcgaac
    15421 cacagcggtg cttcgttgaa aagctgcaac ctatctacta acagatcatt gacgattcca
    15481 taactattat tgaacatata tttccaagca aaaatcaaag aaatagatgg cgtaacataa
    15541 cttaataaaa tcagtgaacg caccgttttc ctaaatctga acttgcggtt aagcaaaata
    15601 gctacggcta atcctaaagc tgtactgcct gctacaacta agaatgtgta agcaactgtt
    15661 aaacctaaag aacgataaaa ttcactatca cttaaaattt caagataatt gcttaatccg
    15721 ataaaaacgg cgtctgagtt aggattcagc ggcatatcga aaaaactgat ctgaatattg
    15781 gaaagcattg gaatcagcac taaaacagct aataaaagca tgcttggtgc taaaagtccc
    15841 atggctaatt ttaagtcaga atgtttcctt ggacgattca ttgaaacctc cccctgataa
    15901 tctgccaata gctatctact tttttcgaac accattggca gaacttcttg ttatttcgta
    15961 tttttttgtg cgtcagctaa agcttgatcg acatcttcgt ttccgacagt tacttggttc
    16021 actgcttttc cgatcacacc tgagcttgtg atatctccca ttttcgtgaa attcttttcc
    16081 ccgactagtc caaagacttg tacttcatca aaagcactag caatctcaga tggcagctcg
    16141 ccaaaagact tgatgacttc attttctttg taaccatcca gttcgactac ttgtttattc
    16201 actggttgag caccaccagg agacatcaag acccactttt ccatgttttt cggttgagat
    16261 aagaaagctg tgaatgcttc agcagcctct ttttgtttct cgtctagacc agaagaaatc
    16321 gtcaatccag atactgtgcc atagacagct tgatcttttt gtgttgggat agcaaaaccg
    16381 atattttctg agttccccgc ttcataaaca gaaggcaaaa tgtaagttga atagattgcc
    16441 attggtgctg ttccattcat gaaagcatct ttgatttctg tcacatcatt agagcctggc
    16501 atcgtgtatt gggataagtt ttgataaaac gacaatgctt ctctcatctt ttccgtatcg
    16561 atcgttacct cgccttcatc atccaaaaca tttgctccat ttgacaaagc aaattgagag
    16621 aacgcttgct cactcattgt tccttcagca gtaggcatag caattccata ttgtttattc
    16681 tctttatctg tgaaatgttg agcaattttt aaaatatcat cccaattttc tggttctgaa
    16741 aaaccagctt ctgataattt ttctttgtca taccaaatac cttgaaccca gccgctaatt
    16801 ggtgcagcaa tatagctttt tccatcttct gaacgaacaa ggtttttggc tccgtcataa
    16861 taattatctt ctccagcttc ctcgataacg ttttggactg ccgtctgatc aatcagttca
    16921 tctttatcca tcactttagc aaaatcctga ctgacttcca ttacttctgg taatttaccg
    16981 gaacgagcta acgtaacgat ttttgtattg aacgctgatt cttctaccgg cacttgcttg
    17041 attttgatgt ctggattttc tttttcaaaa tcagcaacca gttcattgat cacacttaat
    17101 cgttcctgtt cgacagaaga gtgcatgaat tgaatcgttg tctgttgtga ttcgttagag
    17161 ctttgacttt caccacaagc agtaagaaga aatccagtag ctagcagaca ggtagtaaca
    17221 ataatttttt tcattttttt ccacctctta ttttattatt ttcagccaca tatattcata
    17281 cggctggaat atcaactttc gttcttgact gcaatggata atttgtccag aaaacaggtc
    17341 taggtaatct ccttttggta aaagataacc tgctgattga ttggaaaaat tatggataac
    17401 taagactttc tcttccttcg cttcgcgaat aaaactgata aaagaatctg ctgagtccag
    17461 taagtaaatc aaactattcg gatggaaggc tttttctttt tttcgaatag atagcatctg
    17521 ggtcaattca ccatatactg ctgttcttaa tggatctgtc tgcaactctt tctcaatatc
    17581 tgctaacaaa aatttttgcc gattgatttt tcgattctct cctgtttttt ctacgccttt
    17641 gtaatcattt cgacttccca gcacactttg aatataaatt gccggcacgc ctggtaacgt
    17701 taccaaaaat gaatgagcag ctaaaaagcg tttgcttctc cattccgtcg tatctgttgg
    17761 agaacttaac gcatctagat acgtagcgtt tatctcatat ggactggtcg ttccatctgg
    17821 gtttttcttg tagtttactc gggcaccctc tttcttcagc tgttccacta attcaaggat
    17881 cgttttttcc ggcagaatac ctcgtaccgg attcaatccg ataccgtcat gggaggctaa
    17941 aaaattaaaa aaagttgttt ttttcgatgc gaaatcgatg gtttttaacc attctttcaa
    18001 cttacttgta ttacccgaac gaatcgcttg aagggtcaaa ggtggtagcg aaaactgata
    18061 gaccatctgt gcttcattct cgccattccc aaaataactg atattatctt tgtgtggcac
    18121 atttgtctca gtgatcaata ccgttcctgg agctgcttca tcagcgattg cacggaataa
    18181 ttggatgatt ttatgcgttt ctggcaggtg tatacaagaa gttcctagtt ctttccataa
    18241 aaatcccaca gcgtccaagc gaataaatga agcgcctttt tctaagtaaa acaaaagaat
    18301 atcgatcatt ttcaataaaa cttctggatt tttaaaattc aagtctactt gatcttcgct
    18361 aaaagtcgtc catacgtgtt tttctttccc gtttttgtca atgaacgttg taagtaacgg
    18421 gctggtcctt ggtctagtca cgctgcttgt atctgtatcc agtggcaaat caataaaaaa
    18481 atctttgtac gtatcattca agtttaaata ttcctgaaac caagtacttt cggcagacat
    18541 atgattgcag acaaagtcga acattaaccg tgtttcgctt ctcatttctt ctatatcttc
    18601 ccatgtgccc aaacggtcgt tgattgcctg ataatttaca acggagaaac catcatcaga
    18661 tgtatatggg aaaaatggca agaaatgtac aactctaaat gtatctgcca aatacttttg
    18721 ataaaatttc ttaaatgtac gcaaagttgg ttgctgctct tcaaaaaatt gatccccata
    18781 agtaatcaag aaaatatctt cttcgtccca atgcagtttg cctgcctggc ctttcttctg
    18841 ctgataaaca ctgacttttt gttcaatcat agaggcggtt ttctcagaca aatctgagtc
    18901 gcgataaatc gcttgtaaaa gttttgtata catcggattc ccctttcttt tttgggagcg
    18961 ttcccaacga tttgatcata ttatggaaaa cgcttactgt caatagcatt tttttaaata
    19021 attttgatat acacaaaaat acaaataaga aagcttatgt atcaataatt aatagcttct
    19081 taaaaaaata tggtctctca tgatcatgca agctattcct aaattttcaa aatcatagac
    19141 aaaaaagaat gtctattgta ctatggaatt attctaaaac gctcccaaaa aggaggtgca
    19201 tataaaattg aaacctacta tttatgacat tgcgaaaaaa gctaatgtct caaaatcaac
    19261 tgtctccaga gtattaaata accagccaaa tatctctgat aaagcaaaag caagtgtact
    19321 tcaagcaatc aaagaattga attatgaacc gagcaaaatt gcaagaggac tgtcaggaaa
    19381 tggttttgac gcgattctag cattatctca tcgtacttct catacgacct atggcaatcc
    19441 ttttttttca gatgttattc aatccatttc tgccgtttct gaacaaaata acttcgactt
    19501 gattctacaa acggcaaaaa caccagaaga agagctagaa aaatgcgcga aaaagatcca
    19561 agaaaaaatg atccgaggaa ttattatttt aagctctacg atggatgaaa cacttctttt
    19621 tgaattggat caatacaata ttcctattgt ggtaatcgga aaagtcggaa acgactacac
    19681 gaacatctac tcagtggaca cggataattt ccaggatagt tacgatttgg tggaaacact
    19741 gatcaagaat ggacaccgtc atatcggctg tctccatgct ccgacagaag tttatgtatc
    19801 caatgatcgt ctcaatggtt accgtcaatg cttatttgat caccacctag atatccgtaa
    19861 tgaatggatt atagacggcg gctttcgctt agaagatagc atggcagcaa tgaatgattt
    19921 aatgaagcta cgagaaagac caactgcgat tttttctacc gatgcattaa aatcattgag
    19981 tatttacaaa gtggcagaac aatctggaaa gcagatacct gatgatcttt cgatcgcagg
    20041 attcagtgat cccttattgt cctccctatt ttcgccacag ttgactcgga tagacattcc
    20101 gacaaatcag ttaggaatca aagccacaga acttttattc caactaatcc accaccaata
    20161 ccctgaagaa aaaagaatca ttctttcaac cgagattact ctcacagatt cgatccaaca
    20221 acaagaaatg tagaacagaa ttataaaaga acagaaacac tgaggataag tcagttactc
    20281 tgttcttttt tcaatcactc atccctcgtt accccctccc ggtattttag cgaatttttc
    20341 ttattttcca tctgttttag atagctgtgg ataactataa tgtttgtgtt aaactatttt
    20401 ttccacaggg aagaaaaaac tatttttaac cacgttgtgt ataagttttt atagttcgtt
    20461 tttttaaaca catttaccct tatttcaatc catgttattt ggggatactt atctctaaaa
    20521 cataaataga ataacagaca tgtctactgg ttttgtttta ttcgaggaat tattcaataa
    20581 tagctagcaa aatattaaaa ataaccgctc tatcttccca tcaatttatt ttgcattcaa
    20641 aaaatgatct atctttactt ttgttgattg acttctcttg gaagatgatc gttccgatac
    20701 cttaattgga tacgaaccga tgttgttaag atcctttccc acttttcttc tagttagaca
    20761 aattatttta gacaaaccaa aaaagaggtt gtgacaataa tttgtcacaa cctctttttt
    20821 aaaatatcta ttttgtttga ttgttcattg cgtcttctgc agccatttgc gcttcaatat
    20881 ctgaaatact gtagacattt tcgcttgcta caccgacttc ttttggttcc atgtcacgat
    20941 aacgttgcat accagtacca gctgggatga tcttacctat aataacattt tctttcaaac
    21001 caagaagagg atctttctta ccgcggatcg cagcatccgt caagacacga gttgtttctt
    21061 ggaatgatgc agcagataag aaactatttg tttctaatga tgcttttgtg atacctagta
    21121 agacaggtcg agaagttgca ggaacgcctc ctgagactag cgtattgtag ttttgatctt
    21181 taaagtctgc gatgtctaat aatgtacctg gtaagatatc tgtatcacct ggatccatta
    21241 cacgaacttt acgcagcatt tgacgaacca ttacttcgat atgcttgtcg ccgatttcta
    21301 ccccttgcat acggtaaaca cgttgtactt cacgaagcaa gtagttttca acagataata
    21361 catcgcgaac ttgcaacaat tgttttggat cgattgatcc ttctgtcaat ggtgcaccac
    21421 ggtggatcat atcgccttct gctactttca tacgtgctgt ataaggaaca gtatacgtac
    21481 gtgtatctgt ttttcctttg attgttactt ctttggtacg tgttgcagga tcttctgaga
    21541 tatcgatgac atcccctgtt acttctgtga tcactgcttg ccctttcgga ttacgtgctt
    21601 caaagatttc ttggacacga ggaagacctt gagtgatatc gtctccggca accccacctg
    21661 tatggaatgt acgcatcgtc aactgagtac caggttcacc gattgattga gcggcgatcg
    21721 taccaactgc ttctccgact tcaacgtcag agccagttgc aaggttgcgt ccgtaacaat
    21781 gtttgcagac accatgtttt gtattacatg tgaagacaga acggattgtc actgtttcga
    21841 cacctgcatc aacgatttgt tttgcgatgt cttcggaaat caattggttt cctgcaatga
    21901 tcaattcatt tgtttcagga tgacgtactt ctttacgggt gtaacgtccc actaaacgat
    21961 cttctaatga ttcgatgatt tcatttccct cgcggatggc ttggatttca agacctcggt
    22021 cagtaccgca atcttcttca cggatgatta catcttgggc cacgtcaacc agacgacgag
    22081 tcaagtaacc tgaatcggca gtcttcaagg ctgtatcggt catcccttta cgagccccgt
    22141 gagttgagat aaacatttcc aagacggata gtccttcacg gaagttcgaa atgattggca
    22201 attccatgat tcgaccatta ggagcggcca tcaaaccacg cattccggca agctgtgtaa
    22261 agttggaaat gttaccacga gctccagaat cagacatcat gaagattgga tttttcgctt
    22321 ccaaaccttc catcagtttt tgttggatgc tgtcttttgc agcattccaa acagcaatga
    22381 cacgttcata acgttcgtca tctgtgatca gaccacgacg gaattgtttt gtaatagttt
    22441 caacttgttt atgggcattt tcaatgattt cttgtttttc attcagtaca ctgatgtcag
    22501 cgatccctac tgtgataccg gcatgtgtcg aatgtttgta ccctaagtct ttcatacggt
    22561 caagcatctt agaagtttct gttactttga atcgtttgaa gacttctgcg atgatgtttc
    22621 ctaagttttt cttcttgaac ggtaatacta attcttgctc tttgatatgt gcaggaatat
    22681 cagtaccagc ttcaacaaag tatttgtccg gtgtttggac ggtcaagttg aagtctgttg
    22741 gttcattcag ataagggaat tcaggaggca tgatctcgtt gaagatgatc ttaccaactg
    22801 ttgtgatcat cgtacgttct ttttgccatt cagtaaatgg tttttcacct aatgagttag
    22861 ggttcacacc gatacgtgaa tgtaaatgaa catatccgtt gcgccatgcg ataacggctt
    22921 cgttttgatc tctgaagacc attccttcgc cttcgcgtcc ttcttcttcc attgtcaagt
    22981 agtagttacc tagaaccata tcttgagaag gagtaacaac tggtttacca tctttagggt
    23041 tcaagatgtt ttgagcagct aacatcaaca tacgtgcttc tgcttgtgct tcttcattca
    23101 atggaacgtg gacagccatt tggtctccat cgaaatcggc attataagct tcacatacaa
    23161 gtggatgcaa acggattgcg cgtccttgta ctaaaactgg ttcgaatgct tggataccaa
    23221 gtcggtgaag cgtaggtgcc cggttcagca atactgggtg ttccttgatt acgtcttcta
    23281 aaacatccca tacttcgtct tcttgacgtt cgattttacg tttcgcattt ttgatgtttg
    23341 aagctagttc gcgttgaaca agctcacgca tcacgaatgg tttgaataat tcaatggcca
    23401 tttcttttgg aagtccacat tggtacatct tcaagaaagg tccaacaacg ataacggaac
    23461 gacctgagta gtcgacacgt ttacctagca agttttgacg gaaacgacct tgtttccctt
    23521 tcaacatatg agaaagagat ttcaatgggc ggttacctgg tccagtaact ggacgtccgc
    23581 gacgaccgtt atcgatcaat gcatctactg cttcttgcag catacgtttt tcattttgaa
    23641 cgatgattcc aggtgcattc aagtctaaca aacgtttcaa acggttatta cggttgatta
    23701 cacgacggta aagatcattc aagtcacttg ttgcaaaacg tccaccttct aattgaacca
    23761 ttgggcgtaa atctggtgga ataacaggga taacgtccat aaccatccag cttggttgat
    23821 ttcctgaagc gcggaatgct tctaaaatat ccaaacggcg gattgcacgt gtacgttttt
    23881 gtccttgtgc agatttcaat tcttctttca attcggctgc ttctgcatct agatcaacat
    23941 tatcaagtaa ttgtttaatc gcttctgcac ccatagcagc ttggaattct tgtccatatt
    24001 gttcacgttt ttcgcgatat tcgcgttctg tcaacaattg ttttttctct aaattcgtat
    24061 ttcctggttc gatgactaca tatgaagcaa aataaatgat ttcttccaaa gcgcgtgggc
    24121 tcatgtctaa tacaagaccc atacgtgaag gaatcccttt aaagtaccag atatgtgaaa
    24181 caggagctgc taattcaata tgtcccatgc gttcacggcg aactttcgaa cgagtaactt
    24241 ccactccgca tcgatcacaa acgattcctt tataacggat acgtttgtat tttccgcagg
    24301 cacattccca gtcttttgta ggaccaaaaa tccgctcaca gaacaaccct tcgcgttcag
    24361 gttttaaggt acgatagtta atcgtctcag gtttttttac ttcaccgtaa gaccagcttc
    24421 tgattttttc gggagaagcc aaacctattt gcatactttc gaatttattt acatcgatca
    24481 aaaggggttc cctccatttc atttaatggc aaaccgcaag acggctgatc ttcgaccgcc
    24541 ttgcagctca tagaccggat tagtctaaat tatcttctgc ggtttcaaag ttttggatga
    24601 catcgtcttt ttcatcttcc acttgttcag cagctttctt ttctaattct ttggcagttt
    24661 gctgttcggc aaattttgtc aaagcatcaa cagtgatcaa atcgtcgtct tcatcgtcca
    24721 tgtcgcgaag ttcgatctct gtttcttcga tatccaagac acgcatgtcc agacctagtg
    24781 attgcaattc tttcaccaat acgcggaatg attccggtac acctggtttt ggaattggtt
    24841 cacccttgac gattgcttcg tatgttttca cacgtccgac tacgtcatct gatttgtatg
    24901 tcaagatttc ttgtaaagta taagccgcac cgtaagcttc cagtgcccat acttccattt
    24961 caccaaaacg ttgtccacca aattgagctt tacctcccaa tggttgttgt gtaaccaatg
    25021 agtaaggtcc gattgaacgt gcatgcaatt tgtcatcgac catgtgagca agtttgatca
    25081 tgtacatcac accgacagac acacgtccat caaatggttc acctgtacgt ccgtcgtaca
    25141 gaacagtttt cgcatcgctg gccataccgg cttcacgaac tgtttcccat acgtcttcat
    25201 cacttgcacc atcaaatact ggcgttgcta cgtggatacc taactgacgt gcagccatac
    25261 ccaagtgtaa ttccaatact tgcccgatgt tcatacgtga aggcacccct aaaggattca
    25321 gcatgatatc gattggtgtt ccatcaggta ggaatggcat atcttcttcc ggcataatac
    25381 gggatacaac ccctttattt ccgtgacgac cggccatttt atccccttca tggattttac
    25441 gtttttgaac gatataaaca cgaactaaca tgtttacgcc tggtgacaat tcatctccag
    25501 cttcacgagt aaagattttc acatcgtgaa cgataccgcc gccgccatgt ggtacacgta
    25561 atgatgtatc gcggacttca cgagcttttt cgccaaagat cgcatgcaat agacgttctt
    25621 ctgcagataa ttctgttacc ccttttggcg tgactttccc tacaagcaaa tcgccatctt
    25681 ggacttctgc accaatacgg atgataccca tttcgtctaa atcttttaat gcgtcttccc
    25741 cgacattagg gatttcgcgt gtgatttctt caggtcctaa ttttgtatcg cgggcttctg
    25801 attcatattc ttcaatatgg atagaagtgt atacgtcatc ttttaccaaa cgacggctca
    25861 tgatgatcgc atcttcatag ttgtaccctt cccaagtcat gaaaccaact aaaacgtttt
    25921 ggcctagagc catttcgcct tgttccatag atggtccgtc tgccagagta tccccttttt
    25981 cgactttctc acccaaatga acgattggac gttggttgta gcttgttccg gagtttgaac
    26041 gacggaattt tgttacatcg tatttatcta atgcaccgtt gtcgcggcgt acgcggattt
    26101 cagaagcatc aacgtattca acgacaccgt catgtttaca taacaaagca gctcctgagt
    26161 catgcgctga tttgtattcc ataccagtac caacccatgg tgcttgagga ttgatcaacg
    26221 gcactgcttg acgctgcatg ttggcaccca tcaaggcacg gttggagtca tcgttttcca
    26281 agaaaggaat acatgctgtc gcaacggcaa ctacttgttt aggtgatacg tccatgtagt
    26341 ctactttatc aatagatact tctaagtttt cactttgagc acgagccata acaacgtctt
    26401 gagcaaatgt tccatcttca ttcaatgggc tgtttgcttg ggctacgatg taatggtctt
    26461 cgatatcagc tgtcaagtag tcgatttggt cagtaacacg acctgtttca cggtctacac
    26521 gacgataagg tgtttcgata aatccgaatt tatttacttt cgcataactg gacaaactat
    26581 tgatcaaccc gatgtttggg ccttcaggag tttcgatcgg acacatacgg ccatagtggg
    26641 agtagtgaac gtcacgaact tcataaccgg cgcggtcacg agtcaatccg ccaggtccta
    26701 aggctgaaag acgacgtttg tgtgtcaatt cgccaagagg gtttgtttga tccatgaact
    26761 gtgacaactg agaagaacca aagaattctt tgatacttgc cacaactgga cggatattga
    26821 tcaattgttg aggtgtcaat gtttcagtat cttggatcga cattctttca cgaaccacac
    26881 gttccatacg tgctagcccg atacggaatt ggttttgtaa aagttcaccg actgaacgga
    26941 tacgacgatt tcctaagtgg tcgatatcat cgacattgcc gataccttcc atcaagttca
    27001 agaaatagct cattgaagca atgatgtcgg ctggacgaac cgtttttaca gctgcttctg
    27061 gatagccgtt tccgatcacg tttacttcac gttcagggtc tcttggtgaa aatactttga
    27121 tgacttggac agtcattgga tctgtgacca caccgtcttc tgatggatag taagtcactg
    27181 aatttaggcc attatcaatg aaaggagcta gtgtttccat gacttggtga gtcagaactg
    27241 ttcctttttc aacgatgatc tcgcctgttt ctggatcaac taatgtttca gctaatgtca
    27301 gattcaatag acgtgttttc aaatctaatt ttttgtttac tttgtaacga ccaacgtttg
    27361 ctaagtcgta acgttttgga tcgaagaaac gagcattcag caagttacgt gagctgtctg
    27421 ctgtttttgg ttcgcctgga cgcaaacgtt cgtaaacgtc tttcaaacct tcttctgttc
    27481 ttgagtcgct tgcgttttta tgcaagtctt tttcgactgt attgcgtaat gtttcgctgt
    27541 cgccgaagat ttcaaagatc gtatcatcgg aaccaaatcc taacgcacga actaatacgg
    27601 ttaaaggaat cttacgtgta cggtcgatac gtacataaga gatgtctttt gcatcagttt
    27661 ccatttctag ccaagctcca cggttaggga taacagtaga accgaatcct tcttttccgt
    27721 ttttgtcgac ttttccatgg aaatagacac ctggtgaacg gactaactga gaaacgatta
    27781 ctcgctctgc cccgttgatg atgaatgttc cttgttctgt cattaatggg aaatcaccga
    27841 agaatacttc ttgtgctttg atttcgccag tctcgcggtt tgtcaaacgc aatgttacat
    27901 gtaatggtgc agagtagttt gcatcatgcg cacgagcttc tgctactgta tactttggtt
    27961 ctttcaattc ataatctaca aattccaaag atagattgcc attgaagtca tcgattggca
    28021 aaatgtcttc aaacatttct ctaagtccct catctaagaa ccattggtaa gagtctgttt
    28081 gaatttcgat caaatttggt aattccaaca cttcactgat tcttgcgaaa cttctacgtt
    28141 cgcgatgttt tccgtatttt actacgtgtc cagccaagct cttcacccct ctaaaatgtc
    28201 attcaaaaag actacccgat caattgttac aataatattt caaatattat ttttaggtaa
    28261 caacagacat tttttgcggc ttttaggcaa aaaaaaacca aaaatagaac attctcttga
    28321 aaccttactt cgtgagcttc tacttttgtt tccttatatt aaagccgaaa cggacaatat
    28381 ttcgtttctg ccttttcttc atcagatagt ttttgcaact tcatatacta cacttatttt
    28441 ctataaaagt caacacttcc tggcgtttct tctgcttttt cgcaacattt tcttaatgtt
    28501 ttcaatttta tgttttcaat tatctgacaa ttcttaattg acagaccttt ttatctcggt
    28561 tatactttag aaaattcatt ttgtggagga acttctatgc gaaccaacat caaccaaatc
    28621 gcaccaacac aactgaataa cttttacttt agctacttta gataggtttg tattcatcat
    28681 gatggataca aacgttaaac gacagtttgt atctatgatg gctaaagaac tttgtattgc
    28741 agcaaaaagc cacgtagaca aaatgctaag tggctgagca gtgaaaatac aaagtaaaag
    28801 tatcaagcat tctacttttt caatttattt tccgactttt caatccattt ggacattttg
    28861 tctgaatgga ttttttgttt cccaaaatta aggaggaaaa agaaatgaaa aaaaatataa
    28921 tcatcggatt aggcgctgcc ctgctactag ctttaggagg ttgcgcacaa aaagacaatt
    28981 ccgcagaggc atcagatgga caaaaaacaa ttggcgtatt gcaacttgta caacataact
    29041 cgttagatgc tgcctataaa ggattcaaag aaggattagc agaagaaggg tataaagaag
    29101 gcgataatct aacaatcgat tatcaaaatg cccaaaacaa tcaagacaac ctgaaaagta
    29161 tgagtgagaa actcgtcaaa gaagatcctg atttgctact tggaatcgcc actccagcag
    29221 cacaatcttt attaaatgaa acaaccacta tcccgatgac tgtcacagcg gtcacagact
    29281 tggaacaagc ccatttagtc acatccaatg aaaaacctgg cggcaatgtg actggcacga
    29341 cagatatggt gccaatcgaa aaacaaatcg gcttactgtt aaatatcgtg ccagacgcca
    29401 aaacaatcgg catcatgtat aacaacggtg aagcaaactc taagatccaa gctgatttgg
    29461 cagaaaaaga attgaaaaaa gcgggcgtca aagtcaaagt actgacagca aattccacaa
    29521 atgatgtcca acaagtgacg aaaagtctag ctaaagatgt agatggaatc tatattccta
    29581 ctgataacac gttcgcaagt gcggcaagtg ttgtcggtga agtcgcaaaa gaaacaaaaa
    29641 cacctgtcgt tgctggcgca gttgatcaag tcaaagacgg cggtctagca acagtaggaa
    29701 tcgactacga aagcttgggc aaacaaacag ggatcatggc agcaaaaatc ttagacggca
    29761 agaatccaag tgaacttcct gtcgaaacag cgaatgattt ggaactgtat gtcaataaag
    29821 acatggccaa agcattaggc atcaatccag acagcatcaa agaagctgaa taaaacacag
    29881 gaggattctc atgacaaatt tgctctctct tttcttatca tctgcatcgc aaggggtctt
    29941 atgggcccta cttgcgattg gtgtatacgt cactttccgt atcttggaca ttgccgactt
    30001 gactgctgaa ggaagctttc ctttaggtgc agggatcgcg gcagtaagta ttacaaatgg
    30061 ctatcatcca ttgacagctt gtcttctagg ctttttaggg ggtgcggcag cagggcttgt
    30121 ttccggactg cttcacacaa aattgaaaat tcccgccctt cttgcaggga tcatcaccat
    30181 gactggttta tattctgtca catcccgtgt gatgggtgcg ccgaacatct ctttattggg
    30241 tcagccaacg atctttcatt gggtcgagtc attcggcata acaaaagaca acgctgtact
    30301 gatcgtcggt ctgctttttg ctttgatcgt tgtatgttta cttgttttgt tcttacgtac
    30361 agaaacagga cttgctattc gtgcaactgg cgataatctg gcaatgagcg aagcaaacgg
    30421 gatcaacaca gataatatga agatcatcgg ttatatgatt tctaatggat gcattgctct
    30481 ttctggctcc ttgcttgcgc aaaacaacgg ctttgctgat ttaaattctg gtattggaac
    30541 gatcgtaatt ggacttgctt ctattatcat cgcagaagtt ttgttacgta accagccact
    30601 atttctccgt ctcttgactg tcgttttagg tgcggtcatt tatcgtttta ttttagcgtt
    30661 agtcttcgaa ctgaatgttg aaccaagtga ttcgaaactt gcttctgctt tcgtgctagt
    30721 gatctgtctt tctttcccgc agatccagca aaaattccat ttacctcaaa aaaagaaagg
    30781 cggtagtgtc aaatgaatga cgtcttaaca ttggaaaatc tccatcatac ctttgaaaaa
    30841 ggaacgatca acgaaaatca tgtcctgaaa ggcattaatc tttccttaga tcccggcgac
    30901 ttcgtgacga tcatcggtgg aaatggggct ggtaaatcta ctttgctgaa cagcatcgcc
    30961 ggtacatttt ctgtcgatca gggtcaaatt ttattgaatg gaaaaaatat cacgaaaaaa
    31021 tctgttgtcg aacgttcgaa atccatcagt cgcgtattcc aagatcctaa actagggaca
    31081 gccgttcgtt taacggtaga agaaaatctt gctcttgcta tgaaacgagg aaaaaaacgc
    31141 ggcttcttcc gtggtgtcaa accacaagac cgaacatttt tcaaagacca gctggttcgg
    31201 ctaaatttgg ggttggaaaa tcgtttatcc tcagaaatcg gtttgctgtc tggaggacaa
    31261 cgtcaagcta ttacgttatt gatggccaca ttacagcgtc cagaactgat cttattagat
    31321 gaacacactg cagcgcttga ccctgctact tccaaaacgg tgatggaatt gacagaaaag
    31381 ctgatcactg agcagaatct cactgctttt atggtcactc atgacatgga agacgcgatc
    31441 cgctatggga gtcgactgat catgctgcat caaggaagga tcgtcgtaga tatccagcaa
    31501 gaggaaaaac agcagctcac agtgccccaa ttattagcgt tgttcaaaca aaacagcggc
    31561 acggcattaa aagacgatca agtactttta tcttcataat cagaaagagg atgggacaaa
    31621 aaatgaacgc ccaccctctt cttttttctc tttccttctt tcgtttttgc tttatgtatc
    31681 aaccgttgtt tcttcgaaaa ctattttgtt ctctccttct tcctgataga atggatggga
    31741 aaggagcttg atgccatgac aacgaaagac aaggtacttc aattattgaa aaagacacca
    31801 gattttcttt ccggagaaaa gttggcacag caactggaag tttcccggac aagtatttgg
    31861 aaagccatca aagaattgga aaaggcaggc tatcgttttg agcacgggcc tacagggtat
    31921 cgttacctgc cctctgacgt actagatgca aaagaaatcc agcaagagct acggtcgttt
    31981 attcctgatt tgaaagtaca aacgatgttg acttctgaat ctacgatgaa agatgccaaa
    32041 ctggcagcag ctcaaggaga ttctgtttcg aagttatttg ttgctgatac acaagaacaa
    32101 gctcgtggac gttttggacg gccttttttc gctgaacctg gacgaggaat ctacatgagt
    32161 ctcttgctcc atccagacaa acggtttgat gaattgcctc aatatacgat catcgcagcc
    32221 agtgcctgtg tgaaagccat tgaatctgta accggtcgtt ctgcttcgat caaatgggtc
    32281 aatgatatct acatggatgg aaaaaaagtc tgtgggattt tatctgaagc agtcagtgat
    32341 atggaaagcg gcaggatcag tcatatcatc attgggatcg ggcttaattt ttctgttcct
    32401 caaaatcatt ttccagctga tctccaagaa aaagccatct ctgtctttcc cgacggacaa
    32461 gctagtgtca caaggaatca gctaatcagc aaaatctggc aagaattgtt cacttatctt
    32521 tctcacccag aaaaagcgtt ggacttctat aaagaaaaat cattcgtatt gggaaaaacc
    32581 gtgtcgttca atcaaaaagg aacgacttat gtcggtcttg caaaagaaat cggtgatcat
    32641 ggcgaactga tcgtccaaac aaatgaacag aagatgagtt tgtcttctgg cgaaatcagt
    32701 ctcacatcta tccgtacgca ataattatca gatttcacaa aaggacacct gatctcatac
    32761 agtgtccttt tttctgaatt ctcttcaaaa gactctcctt attctatcag ctgacagttt
    32821 ctctatcctc tagaaaaagg tattctaaaa atatctataa agcatggtac actttcttta
    32881 aatgaatcaa tgcaaaaaac aagagagtca tcagtttaca cgatcaaaag agatagcagg
    32941 ccgcttcctt tttcaatctt atagatagac catgggtaac gataaaaaca aaaaagaact
    33001 tgcgacttat tttgccgtaa gttctttttt agctagtgcc ttaatctctt aatcccaaca
    33061 aacgtctcgc atagatcacc tgaccagagt gcatagcagc atcatcaata atagaaacga
    33121 gacgcattcc tcgtgtgact ggtggtgtcc agttctcgtc aattacttca tctagctcgg
    33181 aaggttccag ccttcttaag taagaaatag caaaatcagc agctcctcgc aagtatccta
    33241 gcagatcttc tatttgatta gcttggatct gctttgcttg ttccagacta tgcttccaat
    33301 cttgttcgtt atctgctatc gtcatcggaa accggctttc ccagtcttga ctatgccaaa
    33361 ttggagaagt tcctgctaaa tcagcaatct ggaaatctaa ttctcgagcg gtatgccaag
    33421 ccagccaagt cattgatttg atctgcgggg ctctatcttc cactggaaaa cgattggcct
    33481 cctcaataga gacgccttca aacaaacgtt caaaccgatc gtatgcccga tccacactct
    33541 cgcatagcat atctaaataa tccatttatt ttcctccttt ttatttatcc tacttacttt
    33601 ttcctaattc taggatacct acttatatgt tactacacac ccttattttt tacactttaa
    33661 cagtcaccat catcaatagt ttggagattg aaagaaaaag atagtgtcga actatcaatt
    33721 gaacaagaag caacaatatg aacgtttcat taatattctt taaataaaca atgatttttc
    33781 aaacgaacag tttaagacta ggagaaaaag agtataatag gaaaatgttt gaaaggaaag
    33841 gaaggggaaa aatgaaaaaa agggcaagtt tactagtcct agttgctttg ttgttcaata
    33901 tcttttcgta tggtgtggcc gtttcagcgg aaacatacat tcctaaagac cctgggaata
    33961 tttataatga tctaactggt aactatggct tgttaggaat tgctagtcaa ttccacgtat
    34021 ttgctaaaaa taagacaact gtgaatgcac atacagacgg aaatatagca acacatgaac
    34081 ttgatgcaaa aaataatttt ggtacagagc ttcatgaagg tttattgaac caagaaataa
    34141 attatataca aaaaactgat tctttcgcta attcttcagg catacctaca ggtagcacaa
    34201 gaatgaataa atttgtcgtt ggcagtaacg tggatgtagc acttcaagat ggtcatccta
    34261 cagtcaatgg atccaggctt gatcacttaa ctccagatga tttttataaa gatcgtgctg
    34321 ggcatgaata tattgatttc gagcaggaat ttgctaaatt gaatgtagca gcaaatgatc
    34381 ttgcaacgat cactccagct aaaacatata ctgctgcaga ttttccagac atgaacaatc
    34441 ggacaatcga tttaactgat ttatcagata ccgggttttt attggtaaat atcgatgcag
    34501 aagtgttaac gatgaatacg ccacttcaaa tcattaatcc aaatgatcaa gtagttgtat
    34561 ttaatgtcat caacagttct tctgcattga acgtacagtc accaatcaaa tacaatgatc
    34621 gttcaaatca tgaaacagaa gatttttcgg atgcgaatat ctcttggaat tttggaaatg
    34681 agatgactga tttgacaatc agtgctcctt ttcaaggaac aatccttgcg cctaatgcta
    34741 ctatccgcat tactcaaaac caagacggaa cgattatagg aacaaatgtg attttagatg
    34801 cagctaccaa tcgttgggat cctaacgaaa tatttatcag cgacaatggt acagatacga
    34861 cagacacaag cgacagtaca gatacatcta ccacaagtga tagctcagat tcatcaactt
    34921 caagcgacag tacagatact acctctacca caagtgatag cacggataca tcagcttcaa
    34981 gcgacagtac agatactacc tctaccacaa gtgatagcac ggacacatca gcttcaagtg
    35041 acagtacaga tactacctct accacaagtg atagcacgga cacgtcagct tcaagcgaca
    35101 gtacggatac atctaccaca acaagtgata gcacggacac gtcagcttca agtgacagta
    35161 cagatactac ctctaccaca agtgatagct cagattcatc taccactaca agtgatagca
    35221 cggacacgtc agcttcaagt gacagtacag atacatcctc taccacaagt gatagctcag
    35281 attcatcaac ttcaagcgat agtacagaca cgtcgtctac cacaagcgat acacgtcatg
    35341 caagtgtctc cttctcaaaa acaagttcgg ataacaggac gaactttaac ttaactaata
    35401 aaacaagcaa tgacaatgga aacaaatcaa aaagagcact tcctaaaact ggaagtcaat
    35461 cgaataattg gataacactc gcaggcgtta tattacttgt aatcggttta agaatcactt
    35521 ttatgagtta taagaaatcg aaaaggtaaa aaaatattca agataaaaaa gagcaacgtc
    35581 gctaacggac gttgctcttt atttttgctt tttatcataa atacaagaac tacaattcaa
    35641 tttgtcaaaa aacacgtaga tttccttctg agaaatttat ttacgtatcg aacctttccc
    35701 tattcagtct ttgcgatcca aaatcagttg ggtaagggaa aagagattct cttttgttcg
    35761 aagagaggtg gcaggtaatt tctttatttc cttcaaagcc ttttctgtgt aagaggccgc
    35821 taacttccta gcttcttcca cgcctcctgt atgttcaacg attttgcgta cggcgtcgac
    35881 atcttcttct gtcattgccg ctttcttctc taaataaggt agtagctttg gtttgttttt
    35941 ttgcaaagag tagatcaacg gtaaagaata gaccccttga cgcatgtctt ctagtacagg
    36001 tttgccgatt tcctggctct cttttgtata atctaaaata tcatcgataa tctgaaaggc
    36061 catcccgata gaaagtccga tatctcctgc tttttttgcg aagcgctcgc ttgttccact
    36121 ttcaaaagcg ccaacagaac aacttaattg gaacagttca gctgtcttac ctgaaatatt
    36181 ttccaaatat tcttttacag aaagttcata attatagcga tcatccatct gccctaattc
    36241 gccattcaat actttttcca tactgcgaga attcaattgg agacttttca aggaagtgga
    36301 gtaatcagac aatagtttaa aacagcagac aaataaatag tctcctgcat agacagaaac
    36361 gacattacca aactgactac ggatattagg ctgaccacgt cgcagatccg cttcatcaac
    36421 gatatcatca tggatcagtg tggccgtatg gagcatttcg atagaagctg ccaaagcgat
    36481 ggctttgtct cgatcttgct ctggtccaaa ttgtgagaac aatagctgat aggcaggacg
    36541 caacagcttt ccgccggcat ggatcgtttc tttgattgct tgttcgacag gtttgttttt
    36601 caggcggaca gtttcttcca tccgttttag tgtttctgtc aattctttac ttaaagcagg
    36661 atagttttcc cacattggat gtagcttcat aaaaaaactc cttgtgtgac gattattttt
    36721 tggtaaacat tgggctccat ttttgtttgg ttccctgctc ataatctttt tggaatcgtt
    36781 gaagtgtctg cacatgtcgt aaaagataat tcgctgcgat cccgattgca atccctgata
    36841 aaatccctaa aactgaaagg actggcaagt acaacatgac tgtccatgat tgtgcaatga
    36901 aagaagccat caaaagctgt ccgacattat gcatgaaacc gccggccgca ctaattccga
    36961 tgatactgac acgttttggt cctaattgct tgaccaataa cattccaaaa taactaagca
    37021 aagcaccact catactataa aggaatgtgg aaatcgttcc gcctagcaaa gtagtcagga
    37081 atagtcgtag ccaaagtagt aagaagctgt ctcgtttttt catggtaaaa atggcaacga
    37141 tcgtgattag attggctaag cctagttttg caccaggcgc aaaagaaaaa ggataaggga
    37201 tcatattttc caataaacca atgatcactc cttgtgcaac aagtagcgaa atataaatca
    37261 ttttttgtaa tttactcatg gtgtgtgaag gtctaataaa tcagactgtc ttcgtcacct
    37321 ccatctgttg aacggacttc gatcactaat ttatgtggca aacagacgat ggtttcgcca
    37381 tttttcgttg cccagcctcg acgtacgcag atctggtcat cgcaatctgc ttcttttatc
    37441 cgtactcgat cgccatctat ctcgatcaag ttataatctc cgtgttcatc tttgtaagta
    37501 tatgtgttgc tctcttctcc agccatcaaa gggaatgttt tgatttctgt gccatccaca
    37561 cgcagtaccg cttctttttc tgctgttgcg gtgttttgct gaagaccaaa aatcatgact
    37621 ggtaaaaaag aaagaacagt caaaagcaaa ataataaaaa catcccaagg tttgagcaga
    37681 cttttttcag caaattcttt aaggctcatt tttgttctcc tttttccaga ttaactcacc
    37741 caattttagc ataaaaacaa gaggttgtga cacctgtgtc ttgctttgag taataagaac
    37801 gaagaataga aaacagcttt ccatgttttc taattctttg ggcttattac cgagaagcag
    37861 caggtggaac accgtttata acaagaggtt gtgacagaag tgggcaactt caagaaataa
    37921 gaaggaattt acgaaaatta cttttcaagt ttttgtgaaa tttcggctta tttttcgaag
    37981 aagctacttc tggaacaccg tttataacaa gaggttgtaa cagaggttgt gacacctgtg
    38041 tcttgctcat ggaggcttat ctcgggcatc ttattccttt aattttacat aaaaaggagg
    38101 ctgtgacggt ttgtctcagc ccctttctat tgaaatcttg cattcaaata acgttcttta
    38161 tctatttatt tcttgccgta ctgggaactt ggtctaccgt accaccaatt acctagtgta
    38221 cgttgtagcc atggaattac ccagcgatcc aacccaatcg cacgtccgga accgttcatc
    38281 aatgcgaaag caacaaaaat gaaccaaatg tttacccagt aaaacatgcc tgataagcag
    38341 aacgtgatca ccaatacaat cgttgtcgca ctgcatagcc atgtgaataa tccagcaatc
    38401 aatgctaatg ccaaggcaat ttcaaagaat accatgaatt tttggaagaa cattgctaca
    38461 tcattgtttg gcatgatcac ttgcatgatc tttgtgaacc agtctggtga cttctcaatc
    38521 acagccattg gttcttcacc gtatgcataa ctcaaaccaa aatgtgcagc ttgagtcgct
    38581 gcttctccgg cacttgcccc tgctccgctg gcagctgtgg ttgcatcacc agcagcagaa
    38641 gcaccactgg caacatctgt tacattgtat tcccatgtcc aagggaacac actgtttcca
    38701 gtgaaccatg atccttcacc aaaccaagta cttggtttga acacttcacc attcccaatg
    38761 atttttttgg aagcttcaac gaaccaaact aaaccataga aaatacgaag tggcacactc
    38821 cataagacat tgccgtaacg agaagaatgt cctcgagtca cgttgcgatc atctttgata
    38881 tggaaaaact catgcattaa atattggaac atataataac cagaacgaat atcaaagaag
    38941 tatttcaaat tcacgatatg tttcattacg atagctaaaa atccgcttaa atggatctta
    39001 tcaaataggt tagctacccc ccatttggca ccaatagaaa ccataaatcc ttgatagttt
    39061 cctttaaatt tatgcttttc tccgcctttg atcgaagcca cgatattcgc tgcggctgtg
    39121 tgtcctgttt gttcagcagc ttgaacgatc tgcggtgtcg gtgttcctgg gaattcttca
    39181 taaaagacta aatcaccaat gatatagatg tttttatctt cgtagccttt tgcctgcata
    39241 tattcattgg cgatcaaccg ctgtccgcgt gctgattcta atccaaagtc agcagcgtca
    39301 gaagttcctt tgacaccagc tgtccaaatc agtgtgtgag tcggaatgtt gcgtccgtct
    39361 ttcaatttga tatgatcagc agccacttct acgattggtg cattcaataa taattccacg
    39421 ttgtgcttac gcaaatagcg ttcagctttt gcagcatcat ttcgatccaa catattcaag
    39481 atcgtcggca ttgcctctac gaccatcagt gtgatttcgt ttggatctaa tttataatct
    39541 ttggctaggc gatttttcca gtcgatcagt tccccaatca tttcaatacc ggtaaatcca
    39601 gaaccacaga caacgaaagt cagcatggct ttacgcagtt catcatctgg ttccaatgca
    39661 gcttttgcta ctgtcttctc aatatgttcc cgaatcctta cagaatcttc aaatgaccaa
    39721 agggtgaatc cgtgtgcttt gacaccaggt gtaccaaagt cattcggctc tccgcccatt
    39781 ccaaggatca gctgatcaaa aggataggaa cctaatttgg tcttgacgat ttttcgttct
    39841 ttatcgatac cgattacagt gtctgtgaca agttgtacat tcttttgacg agcaaacaaa
    39901 tgctgcagat cgtattggat tgcttctggt tctacacgtc cgccagctac ttcatgcaac
    39961 tcggtcatca tagtatggta cgaatgacga tcgatcagag taattgctac atcttctttt
    40021 ttcagtttct ttgccaagta ttttgtagct gaaacaccgg catatcctgc tcctacaacg
    40081 acaatatttt gctttgccat tgatcatccg ctccttatag actatctata taatgaatat
    40141 atgataattc ttttagtttg atacttatta cacaatctcc atatattata tagatgttct
    40201 gttcttttgt ctaactcctt ttggattttt ttcaaatttt aaaataaagc gttttttcac
    40261 gaaatttgct gaaagaaaac gtttgaaata ttacatgggt tatttatcaa aaatagttga
    40321 cttataggct aaaaagatta gaatgtataa tgaatcacaa agaaaaattg tggactagaa
    40381 atggaatggt ggggtattaa aatgaagaaa aaacgttttg taactggatt tgccgtatta
    40441 gcattttcag ctctagtgtt aggcgcttgc ggcgcagatg acaacggaag ctcaaactct
    40501 tcttctgaat catctacagc acaatcttcg actgcaaaaa gcacaacaga atcatctgca
    40561 aaagttgtag ctggcggaga tttgcaagac ggcacatata aattagaaga aaaaaattat
    40621 tcaaatggtt accgtgcagt ctttgaaatg gttgtaaaag acggcaaaat cactgaatca
    40681 aaatatgata acgtcaatga aaacggcgaa tcaaaaacaa aagatgcaga atacaacaaa
    40741 aacatggaag caaaatctgg cacaaaccca gaaaaataca ttcctgagtt gaatgaccaa
    40801 ttcttaaaag cacaaagcgc aagcggtgtg gaagtcgtaa ctggagctac tcactcatct
    40861 gaaagcttcc aaaactacgc acaacaattg atccaagctg ctcaagctgg taacacagat
    40921 acaatcgaaa tcgacaatgg tgctgatttg aaagacggca cttacaagct tgaagaaaag
    40981 aactactcta acggctaccg tgtgcaattc gaaatgacag ttgctggcgg caaagtaact
    41041 gaatcaaact ttgattatat cgacaaagac ggtaaatcaa aacaagatga tacagaatat
    41101 aatgaaaata tgaaagcaaa aagcggtaca gaaccaaaaa cttacatccc tactctgaac
    41161 gacgaattcg tcaaagcaat gggcgaagaa gatggctcac ctgctaatgt agaagttgtt
    41221 acaggtgcta cacacagctc acattcattc atcatgtacg cacaacaatt agtgaatgca
    41281 gctgaaaaag gcgacactca aacaatcgaa gtagataata tcgtaacaga aaaataatca
    41341 cataatcata ctaataaaag ttggagggtg ggacgatagc cagattaggc agtcgtctca
    41401 cctttcaaaa tgaagagagg aaaaagatgc ggaaaaaact actattttcc ttaatcacac
    41461 tgggcatctc ttttatggtg ctaactggct gcagcaactc agcaaataaa acaaccactg
    41521 aaagttcagc agctaccaag atattgaaag atccatattc tgatgaacag ttcttgttag
    41581 ggacttacgt tcgtatccgt gtgtatgatg aaggcaaaga atcaacattg aaacctgcct
    41641 tcgatcgagt aaaagaactt ggtgataaaa tcacgatcaa tcaaaaaggt tctgagatcg
    41701 atgaagtgaa tgagcaagct gggatcaaac cagtaaaagt ttctgatgac gtctacacgt
    41761 tagtcaagcg ggcctacgaa tatagccaag attctcaagg cggattcgat atggcgattg
    41821 gtgcaatcac acaattatgg cgaatcggtt ttgatgacgc cagaaaacct tcccaagaag
    41881 aaatcgatca ggcattgaaa ttagtggatt atcataagat cgaactaaat gacaaagaaa
    41941 aaactgttta tctaaaagaa aaaggaatga tcatcgattt aggtgcaatc gccaaaggtt
    42001 acattacgga cgaagtagtg aaagtcttga aaaaacaagg agtgaccact gccatcgttg
    42061 acttgggcgg taatgtctat gtcttaggcc acagcccaag aggcgaaaac caagactgga
    42121 cagtcggcat ccaagatccg aacatggctc gcggatctgt tttaggttct attaaagaac
    42181 ggaacaaaac attagttacc tctggaattt acgagcgtta tttagaagta gatggcaaaa
    42241 aataccatca tttgtttgat ccaaaaacgg gttatccgtt cgacaatgat atcgcaagtg
    42301 tcacgatcat aacagataaa tcaattgatg gtgacgggtt gtctacagca gtcttttcaa
    42361 tgggtgtgaa aaaaggactg gaatacgtcg aaagcgaatt gaataacggg acggaagcga
    42421 tcttcgtgac aaaagacgat aaagtatatg tgaccgatcc aatcaaagac accttcaaat
    42481 tatcggagga ctcccattac acgatgggtg accgttctga actaaaataa ggagtacgaa
    42541 ccatgacact aaaaatcttt ttagaagtag ttgaaatacg aacaaaggtg gctagtgtat
    42601 ttccttttgc ggtaggggta ttattctcta tcgctttttt tcatgagata cattggctaa
    42661 acactttttt attttttatt ggaatgctag tttttgattt agcgactacc gctatcaata
    42721 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
    42781 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
    42841 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
    42901 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
    42961 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
    43021 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
    43081 ttgctttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
    43141 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
    43201 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
    43261 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
    43321 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
    43381 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
    43441 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
    43501 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
    43561 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    43621 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
    43681 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
    43741 cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa
    43801 aatagttcct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
    43861 tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac
    43921 ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt
    43981 attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta
    44041 aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact
    44101 taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc
    44161 tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta
    44221 aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat
    44281 aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct
    44341 gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc
    44401 acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt
    44461 ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat
    44521 gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt
    44581 tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact
    44641 gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat
    44701 aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca
    44761 acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt
    44821 ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca
    44881 ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat
    44941 tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct
    45001 tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt
    45061 tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca
    45121 gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa
    45181 aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct
    45241 ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga
    45301 ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt
    45361 tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa
    45421 tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc
    45481 aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt
    45541 ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt
    45601 gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg
    45661 ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa
    45721 acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca
    45781 gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg
    45841 catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg
    45901 cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc
    45961 tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga
    46021 taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc
    46081 gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca
    46141 taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc
    46201 tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc
    46261 ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga
    46321 catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt
    46381 tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca
    46441 tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata
    46501 agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat
    46561 ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc
    46621 tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg
    46681 cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc
    46741 agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc
    46801 atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac
    46861 tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt
    46921 cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt
    46981 tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat
    47041 catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg
    47101 ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag
    47161 ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt
    47221 tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca
    47281 ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct
    47341 tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca
    47401 cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg
    47461 gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct
    47521 tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct
    47581 tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata
    47641 tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact
    47701 gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt
    47761 ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct
    47821 aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat
    47881 tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca
    47941 atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata
    48001 gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat
    48061 tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg
    48121 tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca
    48181 caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt
    48241 gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag
    48301 caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc
    48361 accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt
    48421 cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat
    48481 tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa
    48541 gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg
    48601 atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc
    48661 tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga
    48721 atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc
    48781 tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa
    48841 ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact
    48901 gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat
    48961 gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat
    49021 gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg
    49081 tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag
    49141 ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc
    49201 cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt
    49261 tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa
    49321 ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc
    49381 atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac
    49441 tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat
    49501 ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg
    49561 gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg
    49621 ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt
    49681 ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg
    49741 tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg
    49801 caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt
    49861 cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag
    49921 catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc
    49981 taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt
    50041 ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac
    50101 tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt
    50161 aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat
    50221 tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt
    50281 tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa
    50341 taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct
    50401 gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg
    50461 gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc
    50521 attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc
    50581 tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc
    50641 aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc
    50701 atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc
    50761 atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt
    50821 ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact
    50881 gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct
    50941 tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg
    51001 ttggatggta aaacgggtat taatcacata gt
//
LOCUS       DAJSMI010000006        42015 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJSMI010000006 DAJSMI010000000
VERSION     DAJSMI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.006"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            341..1951
                     /locus_tag="ON430_000292"
     CDS             341..1951
                     /locus_tag="ON430_000292"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCR3218242.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2243..3112
                     /locus_tag="ON430_000293"
     CDS             2243..3112
                     /locus_tag="ON430_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCR3218243.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3277..4548
                     /locus_tag="ON430_000294"
     CDS             3277..4548
                     /locus_tag="ON430_000294"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR3218244.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTIGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4545..5840
                     /gene="rho"
                     /locus_tag="ON430_000295"
     CDS             4545..5840
                     /gene="rho"
                     /locus_tag="ON430_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR3218245.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            5939..6202
                     /locus_tag="ON430_000296"
     CDS             5939..6202
                     /locus_tag="ON430_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR3218246.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6647..7096
                     /locus_tag="ON430_000297"
     CDS             6647..7096
                     /locus_tag="ON430_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3218247.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7153..8571
                     /locus_tag="ON430_000298"
     CDS             7153..8571
                     /locus_tag="ON430_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3218248.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8571..9431
                     /locus_tag="ON430_000299"
     CDS             8571..9431
                     /locus_tag="ON430_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR3218249.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9605..10378
                     /locus_tag="ON430_000300"
     CDS             9605..10378
                     /locus_tag="ON430_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3218250.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10525..10890
                     /locus_tag="ON430_000301"
     CDS             10525..10890
                     /locus_tag="ON430_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCR3218251.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11038..12246)
                     /locus_tag="ON430_000302"
     CDS             complement(11038..12246)
                     /locus_tag="ON430_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3218252.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12265..12543)
                     /locus_tag="ON430_000303"
     CDS             complement(12265..12543)
                     /locus_tag="ON430_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218253.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            12697..13392
                     /locus_tag="ON430_000304"
     CDS             12697..13392
                     /locus_tag="ON430_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3218254.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(13834..14631)
                     /locus_tag="ON430_000305"
     CDS             complement(13834..14631)
                     /locus_tag="ON430_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCR3218255.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14628..15812)
                     /locus_tag="ON430_000306"
     CDS             complement(14628..15812)
                     /locus_tag="ON430_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR3218256.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(15837..17084)
                     /locus_tag="ON430_000307"
     CDS             complement(15837..17084)
                     /locus_tag="ON430_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR3218257.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17299..19044
                     /gene="dnaX"
                     /locus_tag="ON430_000308"
     CDS             17299..19044
                     /gene="dnaX"
                     /locus_tag="ON430_000308"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR3218258.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19064..19378
                     /locus_tag="ON430_000309"
     CDS             19064..19378
                     /locus_tag="ON430_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR3218259.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19403..19999
                     /gene="recR"
                     /locus_tag="ON430_000310"
     CDS             19403..19999
                     /gene="recR"
                     /locus_tag="ON430_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR3218260.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20063..20707
                     /locus_tag="ON430_000311"
     CDS             20063..20707
                     /locus_tag="ON430_000311"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR3218261.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            20722..21051
                     /locus_tag="ON430_000312"
     CDS             20722..21051
                     /locus_tag="ON430_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCR3218262.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21065..22003
                     /gene="holB"
                     /locus_tag="ON430_000313"
     CDS             21065..22003
                     /gene="holB"
                     /locus_tag="ON430_000313"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR3218263.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22039..22863
                     /locus_tag="ON430_000314"
     CDS             22039..22863
                     /locus_tag="ON430_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCR3218264.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            22856..23203
                     /locus_tag="ON430_000315"
     CDS             22856..23203
                     /locus_tag="ON430_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCR3218265.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23272..24144
                     /gene="rsmI"
                     /locus_tag="ON430_000316"
     CDS             23272..24144
                     /gene="rsmI"
                     /locus_tag="ON430_000316"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR3218266.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24253..25374
                     /gene="dinB"
                     /locus_tag="ON430_000317"
     CDS             24253..25374
                     /gene="dinB"
                     /locus_tag="ON430_000317"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR3218267.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25428..26030)
                     /gene="nrdG"
                     /locus_tag="ON430_000318"
     CDS             complement(25428..26030)
                     /gene="nrdG"
                     /locus_tag="ON430_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCR3218268.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26345..28534)
                     /gene="nrdD"
                     /locus_tag="ON430_000319"
     CDS             complement(26345..28534)
                     /gene="nrdD"
                     /locus_tag="ON430_000319"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR3218269.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            28833..29627
                     /locus_tag="ON430_000320"
     CDS             28833..29627
                     /locus_tag="ON430_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218270.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29617..31659
                     /locus_tag="ON430_000321"
     CDS             29617..31659
                     /locus_tag="ON430_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218271.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            31940..32089
                     /locus_tag="ON430_000322"
     CDS             31940..32089
                     /locus_tag="ON430_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCR3218272.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            32100..33614
                     /gene="dltA"
                     /locus_tag="ON430_000323"
     CDS             32100..33614
                     /gene="dltA"
                     /locus_tag="ON430_000323"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCR3218273.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            33611..34816
                     /gene="dltB"
                     /locus_tag="ON430_000324"
     CDS             33611..34816
                     /gene="dltB"
                     /locus_tag="ON430_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCR3218274.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            34861..35094
                     /gene="dltC"
                     /locus_tag="ON430_000325"
     CDS             34861..35094
                     /gene="dltC"
                     /locus_tag="ON430_000325"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCR3218275.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            35097..36365
                     /gene="dltD"
                     /locus_tag="ON430_000326"
     CDS             35097..36365
                     /gene="dltD"
                     /locus_tag="ON430_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCR3218276.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            36506..37588
                     /locus_tag="ON430_000327"
     CDS             36506..37588
                     /locus_tag="ON430_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCR3218277.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            37588..38391
                     /locus_tag="ON430_000328"
     CDS             37588..38391
                     /locus_tag="ON430_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR3218278.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            38461..39201
                     /locus_tag="ON430_000329"
     CDS             38461..39201
                     /locus_tag="ON430_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3218279.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            39212..40216
                     /locus_tag="ON430_000330"
     CDS             39212..40216
                     /locus_tag="ON430_000330"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCR3218280.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(40261..41958)
                     /locus_tag="ON430_000331"
     CDS             complement(40261..41958)
                     /locus_tag="ON430_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218281.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        13496 a         7367 c         8733 g        12419 t
ORIGIN      
        1 acttaaaatt tttcagtaac gattttctga tttgcttagg tttaacgtta gctatgttta
       61 tatcaagctt attatgtggg ttaaattttt tttgcactca taaaggactt aagaaataat
      121 ttctcaaagg atatcgaaaa agaaaaaatt gtaagcggat agtattcatt tgagtacttg
      181 actttccctt agtcagcttg tattatacac cttgggcacc cttttagagg gtcagaagtt
      241 tacagaaatt tcggctccct attcttattg aagaataggg agctttgttt attgtctgag
      301 acttttcctg cacataaatt aaaaaggagt ttttgaactt atgacaaaat atatttttgt
      361 gaccggcggt gtcgtttcat caatcggaaa agggattgtt gcagcttctt taggacgctt
      421 attgaaaaac cgcggcttaa aagtaactat ccaaaaattt gatccataca tcaacgtgga
      481 tccggggacg atgagcccat atcagcacgg agaagtattc gtaacagatg atggtgctga
      541 aacagatttg gatctgggcc actacgaacg ttttattgac atcaatttga acaaatactc
      601 caacgtgacg acaggaaaaa tctattctga agttttgcgt aaagaacgta agggcgaata
      661 cttgggagca actgttcaag ttattcctca tattacaaat gagatcaaag aaaaaatcat
      721 gcgagcagct aaaatgactg attcagatgt gatcattaca gaagtagggg gaacagtagg
      781 ggatatagaa tctttgccat tccttgaagc acttcgtcaa atgaaagcag atgtcggtac
      841 agataatgtt atgtatatcc atacaacatt gattccttac ttgaaagctg ctggagaaat
      901 gaaaactaaa ccaacgcagc acagtgtaaa agagctacgt ggtttaggta tccaaccgaa
      961 tattttggtg gtacgtacag agcaacctgt ttctcaaagc gtgaaaaaca aattggcaca
     1021 attttgtgat gtcgaaccag aagcagttat cgagtcgcca gatgtagata cactttactc
     1081 catcccatta gttttacaag cacaaggaat ggatcaaatt gtttgcgacc atttaaaact
     1141 agatacacca gaagcagaca tgacagaatg gaaagaatta gaagaacgtg tcttgaactt
     1201 gaagaaaaaa gtacggattg cgcttgtcgg taaatatgtc gagttaccag atgcatatat
     1261 ctcagtagtt gaagcattga aacattctgg tttcgcctat aattcagaca tcgagatcga
     1321 ttggatcaaa gcacaagaat taacgagaga aaatgtagaa gaacgtttga aagacgcaga
     1381 tggaatcttg gttcctggtg gatttggtga tcgcggagta gaagggaaaa tcgaagcgat
     1441 tcgttatgct cgcgaaaatg acgttccgtt tttaggtatt tgtcttggta tgcagatggc
     1501 ttgtgtcgaa tttgcacgta acgtcgttgg attagaagat gcgtcatcag ctgaaacgaa
     1561 tccagataca gcaaacaata ttattgattt aatggctgat caggaaaaca tcgagaattt
     1621 gggtggaact ttacgccttg gattataccc atgtaaaatc aaaaaaggaa cgaagacggc
     1681 tgaagcatat gatggagccg atgttgtgca agaacgtcac cgtcaccgct atgagttcaa
     1741 taacaaatat cgtcaattgt ttgaagaaca agggctagtc ttttcaggtg tctcaccaga
     1801 taaccgttta gtagaaatcg tagagctgcc agagaaaaaa ttctttgtgg gatgccaatt
     1861 ccacccagaa ttgatttctc gtccaaatcg cccgcaaaaa ttaatcaaag catttgtcgg
     1921 agcatcatta gcagatcacg aaacaaaata atttttgaac ttgatagaaa gagaaaagta
     1981 gcaagagaag gggataagca attttcttgt cacttttctc tttttctgat ttgttattat
     2041 ttgtaggaaa aaagatagaa agcaaaagac ttttttcgat taaagcgcta ttggatattt
     2101 tattttttgc aaaaaacttc ttgaaaattt tcattttcat tgattttagt agaaaactac
     2161 cgaaatgttt ggtaaaatag ggtcgttggt taagaattgt cttaacaaat ttagttttac
     2221 aaaacttagg aggaatttta ttatgccagt agtatcagga gctgaatttt taaaagctgc
     2281 tcgtaaaggc ggttacgctg tcggcggatt caacacaaac aacttggaat ggactcaagc
     2341 aatccttgaa gcggctgaag caaaacaagc accagtactt atccaaacat caatgggtgc
     2401 agctaaatac atgggtggtt acaaaatctg taaagattta atcactgatt tagttgaagc
     2461 aatgaacatc actgttccag ttgctatcca cttagaccat ggtgattacg aagctgcttt
     2521 ggaatgtatc gaattaggct atacttcagt aatgttcgat ggttcacatc taccatttga
     2581 agaaaacttg aaattagcta aagacgttgt tgaaaaagca catgcaaaag gtatctcagt
     2641 tgaatgtgaa gttggttcaa tcggcggaga agaagacgga atcatcggta acggcgaatt
     2701 agctgatatt gaagaatgca aacaaatggt tgctacaggt atcgactact tagcttgcgg
     2761 tatcggtaac atccatggtc aatatccaga aaactggaaa ggattagctt ttgaccactt
     2821 acaagcaatt gctgatgctg taggtccaga catgccttta gtattacatg gcggatcagg
     2881 tattcctcaa gatcaaatcg aaaaagctat ctcaatgggt atctcaaaag taaatgttaa
     2941 cactgaattc caattatcat ttgctgcagc tactcgtaaa tatatcgaag ctggtaaaga
     3001 taaagaaggt aaaggattcg accctcgtaa attattagct ccaggtaaag cagcaatcgt
     3061 tgaagatgct aaatctcata tcgactggtt cggttcagct aacaaagcgt aagcatccac
     3121 ataacagata cgatatatag gaatggagaa cgacttttga agtcgctctc cattcttttt
     3181 tacttaaaac ggcagaccat gctcgtttct tttttttatc tatgataaaa tacaagcata
     3241 tccatgaaaa aagattaata aacaaggtgg aaacagatga aaaaaatcgt aatcaaagga
     3301 aaccgacctt tagctggtga agtaacgatc agcggtgcaa aaaatagtgc agtcgcattg
     3361 attccagctg caatcttagc tgattcccca gtgatcttgg aaggtgtgcc ggacatacaa
     3421 gatgttcatt cattaattga aattttagag atcatgggcg caactgtcca tttttccaac
     3481 aacatactag aaatcgatcc tcgagaagtc gtatcgattc cgatgccgca cggaaaaatc
     3541 aatagtttgc gtgcttctta ttattttatg ggaacactgc ttggtaaatt tggcgaagcg
     3601 gtagtaggcc tgccaggtgg ctgttattta ggccctagac cgattgacct acacgtcaaa
     3661 ggatttgaag ctctaggtgc tgaagtgaca aatgaacatg gtgccatgta tttacggaca
     3721 gaaaataaag gattgcgtgg caatcggatt ttcatggacg tggtttcagt cggagcaacg
     3781 atcaatgtga tgttagctgc tgtaaaagca aaaggacaga cagtaattga aaacgctgca
     3841 cgagaaccgg aaatcataga tgtggcaact ttactgaata atatgggggc aaaagttcga
     3901 ggagcgggta cagatgtgat ccgtatcgaa ggagtcgaaa cattacatgg ctgtcggcat
     3961 ttcatgattc ctgaccgcat cgaagcagga acctatctag cattagcagc tgcagtagga
     4021 aatggtatca aagtgaaaaa tgtgatcttt gaacatttgg aaagctttat agcaaaattg
     4081 caggaaattg gtgtccatat gaaaatcagc gaagatgaaa tcgaagttta cccctcgaaa
     4141 gaattaaaac ccgcaaacat catgacctat ccttatccag gatttgccac agatctgcag
     4201 cagccactta ctgcgttact attgatgact ataggcactt cagaaatcat cgatacgatc
     4261 tatgcgaaac gtgtgaacca tgtgccggaa ctagcacgaa tgggcgctga tattaccgtg
     4321 gaaggaaata tgatcatcgt gaatggtcca aataaacttc acggcacaga agtcgtggct
     4381 tctgatcttc gggcaggtgc ttgtttagtg attgctggac tattggcaga agggacaacg
     4441 acgatttaca atgtagatta tatcttacgc ggttatgatc atattatcga gaaactaaca
     4501 gccttagggg catcaattga aatgatagag gaagtgggag ctgaatgagc gattatttga
     4561 caatggcaga actagagaac aaaacgttaa aagaagttta tagttatgcc aaagaattta
     4621 aaattcctta ttacagtaag atgaataaaa aggaattatc tctagctgtg attcgtgcac
     4681 aagcagagaa acaaggattt tattatatgg aaggaatatt ggatatcgtt ggtcaagatg
     4741 gttacggttt tttacggcca atcaattatg gaccaagtgc agaagatatc tatatttctg
     4801 cctcacaaat cagacgattc agtttgagaa atggggacaa agtggcagga aaagcccgtc
     4861 cacctaaaga atcggaacgt tattatggtt tgatgcacgt ggaaagcgtt aatggaaaag
     4921 atccggaaga agcaaaagaa cgtccgcatt ttccagcctt aacacctctt tatccgcaaa
     4981 gacagcttca actggaaaca acaccgcaaa gactatctac ccgaatgatc gatatctttt
     5041 ctccaatcgg ttttggtcag cgtggattga tcgtcgcacc gccaaaagca gggaaaactt
     5101 ctgtattaaa agaaatcgca aatggtatca ccgaaaacta tccggaagta gaattaatcg
     5161 ttctactgat tgatgaacga ccggaagaag taacggatct agaacgaagc gtgaaaggcg
     5221 acgtggtatc ttctacattt gatctgcagc cgcaaaacca tactcgagtt tctgaattag
     5281 tattggaacg cgcaatgcgt ttagtcgaag ataaacgaga tgtcgttatc ttgatggata
     5341 gtattactag acttgctcgg gcctataact tagtcgttcc accaagcgga cgtacattga
     5401 gtggcgggat tgacccagcc gcattgtata aacctaagaa atttttcggg gcagcaagaa
     5461 acatcgaaga aggtggcagt ttgacgattt tagcgaccgc tttagtcgat acaggaagcc
     5521 gtatggatga cgtgatctat gaagaattca aaggaactgg aaatctagaa ttacaacttt
     5581 cacgtgaatt atctgaacgt cggatcttcc cagccatcga tatcaaaaaa tcaggtaccc
     5641 gaaaagaaga attattgatg ccaagtgaag aattagaaga aatttggaaa cttcgaaaaa
     5701 atatgactgg agattcatta gaatatacgg aacaactgat ccgctttttg cgtaaaacaa
     5761 aaaacaatca gcaatttttc aaagaatttc aagatgtctc ctttggtaaa aaaaatccga
     5821 ccagaaatct gaaaagataa ttgcaacctt gactgaaaca tgttaagatg ttaatgttgt
     5881 aaatcgacaa atcaactctg gttcagcaaa tggagcaggg caaaggagcg aaaaaatcat
     5941 gaaagaaaac attcatccag attaccaccc agtagtattc atggattcaa caactggttt
     6001 caaattcttg tcaggttcaa caaaaacttc acaagaaaca gttgaatggg aagacggtaa
     6061 tacataccca gtcatccgtg tcgaagtaac ttctgactca catccattct acactggacg
     6121 tcaaaaattc acacaagcag acggacgtgt ggaccgtttc aacaaaaaat acggtctcaa
     6181 agacgcaaac gctgcggaat aataaaaacg ctgataactc aacgtttagc agactatctg
     6241 atagggatag tctgtttttt tatgtttttt gccaatttaa aacttcgttt gatgctgaaa
     6301 aagtcgtagg atcataatgc acaaaacaat gaaaaactag tatatagttt taattatcca
     6361 cttgataaaa acaccttata ttaacgaaat gaagaggggt tatttcgaag taaaaaacca
     6421 tttacctaaa attagaagct aagctttcct ttcaaggaag gctttttgtt ttgttataga
     6481 cttgcgattg tttgtgagct agatcgttca ttttaatgac ttatacacgc ggatgatggg
     6541 tctttttcct ctgttcattc ataatattga acggtacagc tagcgatgct atactgacct
     6601 cagttccaag aaaacgttaa tattttttgt gggggagagg ttttttatga ggtttgatca
     6661 attgatcgca gaagaactga ttgttttcga tccatcgata gattcaaaag gatcattatt
     6721 tgagcgaata ggggatgtgt tgaaaaaaac aaataaggta agaaatgttc aaaaagtcgt
     6781 taaggcacta ttcaaacgag aggaggagat ctccacagga atagaagatg gatttgggat
     6841 tccccatgca aaaagcaaat atgtgacaaa accagcactg tgtttttttc atacagccaa
     6901 attaacggac tatagcggga tagatgaaat gccaattgaa tatatttttg ccatcgtagt
     6961 tcctgaaaaa tcagcagata tgcatctaga gattctcagc acactctcca gacgattgat
     7021 gaatgcagat tttagaaagg gaataaaaga agccactacg ccagcagata tcatcagaca
     7081 gattagtcaa gaatgaaaat agtagagtct ttgtccgagt atcgatagtg aagaaaaagg
     7141 aggaaaataa tcatgaaaat cgttggtgta actgcttgcc cgactggaat tgctcatact
     7201 tatatgtcgg cagaaaaatt agaagtaaca gcaagaaatc taaaccatca agcaaaattc
     7261 gaaacacaag gtgtaaaaac agaaaatcag ttatctgaac aagaaatcaa agaggcagat
     7321 gcaatcattc ttgcagtaga taaagaaatc gaattggacc gatttgccgg gaaaaaagta
     7381 aagcgtgtct ctacaagccg agcaatcaaa gaaccgcaag tagtaataga cgaagcattg
     7441 agagatattg gcgtattcgt tgtgtctaat gaaaaagaac cagctgcaaa tgagaaaaag
     7501 ccgacaatct ataatcattt catgaatggt gtgaattaca tgcttccatt tgtgatcgca
     7561 ggcggaatca tcatcgcact gagttttgcc tttgggatta cagcggctga tcccaactca
     7621 gcagactata atgtgttagc agctgcattt tcaaggatcg gaggagatac agcgtttgcc
     7681 atgatggttc ctgcgttagg cgcggggatt gctacatcga ttgctggaaa agcaggtttt
     7741 gccccaggaa ttgttgccgg attactggcc tctacaggtg gttcaggttt tcttggcgga
     7801 atgattggtg gcgtgctagc agggtatatc tgtgattttc tggcaaataa aattagcgta
     7861 aaaaaagaat tttctgctat ttatcagctg attgtcgtcc cctttttatc gatcttaatt
     7921 attgggcttt tgatggtatt tgtgattgaa caaccgatca catgggtgtt ggatgctttg
     7981 acaaattggc tgaatagctt gggaaataca tccgggctat tgttcggatt acttatcggg
     8041 atcatgatgg cagctgatat gggtgggccg atcaataaat cgacttcgac tttctcgatc
     8101 ggtttgatgt cagctggagt aaatgcaccg attgctgctt gtatggctgc aggaatgaca
     8161 cctcccctag gtattgcttt agccacttta ttattcaaac ataaatttac caaagaagaa
     8221 cggacttcag gtcaatcatg ctggatttta ggcgcttcgt atattacaga aggtgctata
     8281 ccgtttgcgg tatctgaccc gatccgcgtg atacctagtt tgatgacagg ttcagcagtc
     8341 gctgcaggga tttccatggc agtaggagtt acttcaatgg cacctcatgg agggatttgg
     8401 gtgatgctaa ttccaaatgt tatgaacaat ttacctatgt atgtcttggc aattgcatca
     8461 ggtacagtca ctacagcctt tatgcttgga ctattgaaaa aaacagtgaa agagaaaaaa
     8521 caagacaagt ggacgaaaaa tgaaaatcta acaaatgagg tggaagcgta atgttatata
     8581 caatgaaaga gttattgaaa gtggctaaag agaatcaatt tgcagtacca gccttcaata
     8641 tttgcagcta tgatatgatg aaagcaatca tcgaagaagc agagagatta gaagcaccag
     8701 taatcttaga aattcatcct gatgaaatcg cctatttaag tgatgagttt gttgcggcag
     8761 taagagccta tgcttataca agtaaactgc cgatcgttat ccacttggat catggcggta
     8821 cgatagaaga tgtcttacgg gcgattcgaa acggctattc ttccgtgatg atcgatgcgt
     8881 ctttgaaatc attcgacgac aatgtacaga ttacgaaaaa agtggttgcg ttagctcatg
     8941 cggtaaatgt ctcagtggag gctgagcttg gaacgattgg gaataatggt tctgctgaag
     9001 gaggtgctga aacaatcatt tatacagatc ctgatcaagc gaagattttt gtgaaagaaa
     9061 caggaatcga tacacttgct gtagcaattg gaacagcgca tggcttgtat ccgaaaaaca
     9121 gaactccaaa attaaatatc gacttactga aagaattgaa ccaacaaatc gatcttccat
     9181 ttgttttaca tggcggttca ggtaatccgg acgcagaagt cagcgaagca gtaaaatacg
     9241 gagttgccaa agtgaatttg agctcagatc ttaaaagcgt ctttttcaat gctttgagag
     9301 aaatattgag tgaaaatcca tcgatgtacg agccgaatgc aatttatcca gcagctaata
     9361 aaaaggtaca agaagtggtc aaacataaaa tggaaatttt gaacacagta ggcaaggcta
     9421 gtttatacta aaaataaaaa tgtgtggtca agagggtatg acaatagtct tgtcataccc
     9481 tcttaaccac acacgatgtt cagaagtagt tttttcgaaa aatttcttct tatttctcga
     9541 agccaatcac ttctgtcaca acctcttttt tcctgttact atataaagta aatggggtga
     9601 ttttatgttg aatgaagtgg agaatagaca gcaggagatt gtgaattatc taaaaaacaa
     9661 ccaatttgca agagtcaccg atttgatcga gttagtaaat tatagcgaag ccacgattaa
     9721 acgtgacctt gtttttctgg aaaaaagtgg attgatccgt agagttcgcg gtggcgccat
     9781 gcttattgac aatcgaaaaa ttgacgtgcc ttacctgatg aagatcacca aattagaaga
     9841 agaaacagaa aaacgtaaga ttgcagatac agcttcttct ttgattcgag atgatatggt
     9901 cttattcttg gattccagta caacctcttt acacttagtc agaaacctag caagatttga
     9961 tggtttgcag atcattacga atggcatcgt cacagctgct atgttgagtg aatatacaac
    10021 ggcaaaagta tctgtattgg gaggggcaat tgttccgaaa cgagcaacaa tcaacggttc
    10081 aaaagcctat aacgatgcat tgtcatatta tgcggattta gcgataatga gttgtcgcgg
    10141 gctagatttc agccaaggag taacagaaac tcatgaagga gaggcaatgg tcaaaaaagc
    10201 ttttagaaaa caatcagacc ggcttgccgt tttagttaca cagcaaaaaa ttgcacataa
    10261 attcatgcat caagccattg cttttcatga aatcgattat ctggtgacaa gtaaagaact
    10321 gacaaaagaa cagtatgcga aagtaaagga acaccatatc gaatgtctgt acggataaat
    10381 tattacgtga atgttctaag tggcttcctc atttttagat aggaccttat ctttgctcgc
    10441 ttctctcagc cgatggtata ataatgaaaa ggttatcttg ttagataatc ttactttttt
    10501 atccaggaga aaggagtgca gaaaatgaaa catgtgaagg cgttggtagt gaaggcaatc
    10561 atgatctggg cgattctgtg ggtcgtgctg acaggtttat acggtgttag ctttatggat
    10621 tcaacgattg ttggggtaat tattgtagtc atgatctatg tgctagggga tctgttgatc
    10681 ttacgtaaag tagggaacat cgcagcgacg atcgcagatg ctggttcagc tgcagttatc
    10741 ttgtggctct atctatattt tatgaatgat acagtcgata tttggatgaa agtattgatt
    10801 cctgctctat tgatcggtgt ggcagaatgg ttcttccaca agtggttgct aagtcaaggc
    10861 atcgtaccag atgaacggaa aatggaataa atcaacagtt aaactattct tatcgataaa
    10921 tgaaagcttt aaaaataaat agatgtttaa ttaagaataa tcataaacta tttttactca
    10981 aaaaaccgct cagattattt tcagatctga acggtttttt ttatctattc atcaagatca
    11041 aatttcattt ttcaatgatt caatcaattc gccctctgct tcatgactat gttttcggcc
    11101 attaacaaga tagagtgctg tacatatcaa tggaatgact ccggcaatca catacaatcc
    11161 tctaaatgaa gtaagagatt ttagcatccc catcaaataa ggaccaacgc ctaagcctag
    11221 gtctaaacca ataaagtagg tagataaagc aatactgatt cgatgttcgc ggacgatttt
    11281 taaacaaaca gcctgtccat ttgacataaa cgtaccataa cctaggccga ctaatgctcc
    11341 agagataagc aatgtcacac tattcgtcgt tacactcagt aaaaataatc ctgctgttaa
    11401 gaataaatag ctaggataca tcacgtattg ttctcctttg acatcgaaaa tccttccagt
    11461 caatggacgt gtaaatgtga tgaccaaagc atatacgacg aagaaaaatg acccagcagc
    11521 aactaagtgg attacttttg tataagaaga taaaaatgat aaaacacttg aataagaaag
    11581 acccatcaaa aaggcaattc ctgtaatgaa aagtgctttc ttttcaataa aactattgat
    11641 attccatgag cgaagagata ttttttgttc ttcagataat ggaatatttt ttacaggaaa
    11701 gacaaggcag gcaatcgtaa caaagaagac cagtacaatt gaaaaccaga taatgaaacg
    11761 aaaatcagtc atattcagca aaatcattcc taaaaatggc ccaacagcgg cagctaaact
    11821 agtacttaat ccataataat tgatgccttc ccctttttta gatgcaggga tatatgcagt
    11881 aacgatagca tttgtagctg tagaaactgt accgtagccg aatccattca agaatcgaac
    11941 taaatacaat gccccgatag ttggcgtata aagatatgct aatgttgaaa ggagatagaa
    12001 gatcgcgccg cctcttaagg taaacttccg accaaatagt tctaactgtt tccccatgaa
    12061 taagcgtgcc agcaatgttc cgatgatata gatcccagaa gcaaaacctg cttggctcaa
    12121 gctggcgtgt aaggtgtctt gcgcaatgac tgccatgatg accatcaaca gataataaat
    12181 caggtagaca ataaaattga tcaatgtgat tccaataaaa tatttattaa ataatttctt
    12241 ttccatgtat gttatacatt ccccttaagc tgaatatgct tttaattttt gtatttgaac
    12301 ttctacatcg tgataattgg atggatagcg atcaacaagt tggcgaataa ataaatctct
    12361 tgtagacata aacagattta aaaattcttc gcattcgttt gttggattct catccactgc
    12421 tttgatcaat tcgatcgagt tttgatacgc atcatttaaa aagactaaag aagctttcat
    12481 atcaaatctt gtttgatatg ctgtttctaa tcgattaatg attgtgttca tccagtaatt
    12541 catgtttttt ccctctattc ttaaaaaatt ttacttctgc agtataacga ataaaaaaaa
    12601 ggaaaaaaat cgcataagtg atggaaaaag gtacttaaca aatgttaata tcattagtaa
    12661 aatacctttg gactttaaaa agttttaaag tactgtatga tctttttaga tgaaagtttg
    12721 gatgggggag catcattgga tagtagagtc ttaaaggaat atttggataa aaatgatttt
    12781 ccgattatac gcaaaggata tcataaatat ttgacattcg aagggatgga agattcccat
    12841 acgtatatct tgaaagaggg aacggtaaaa gcaagtgtta tttcaaatga tgggagagaa
    12901 tttaaccttc gctatatcaa agatcttgaa atcgtctctc ttttaaaaga cgaatattca
    12961 caattcatcg attcgccgta taatattcga gttgaatcag aatatgcaga gttttatcga
    13021 atcaatcgtg tgcgcttttg gcaaaatatc aaccaagacc caaagctaca acactatgta
    13081 aaagagtatt accgctatcg tctgctttat tcgatgaaaa aaatgcagca aatgctatta
    13141 aacggaaagc tgggagctgt ttgtacgcaa ctttacgaat tatatgattt attcggtgtg
    13201 cggacagaag aagggatgct gatcgatttt tcgatcacga atgaagaaat cgccaaattt
    13261 tgtggcatct caactgctag cagtgtaaac agaattttaa aacaactgaa aaaagatggt
    13321 attatcaccg ttaaagacca tcatatcgtt attttgaata tggacttgtt agaagacaat
    13381 attatctttt gatgaacaaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa
    13441 acaaacggaa aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc
    13501 tgattgaaca ccgtttacca agaggttgtg agcctgccgt ttaactctga gtcacaagga
    13561 acaatttcaa aaaacagctt ttcatgtttg atgaatattg tgacttgtga ccgaggagtt
    13621 ggtaggagaa caccgtttac caagaggttg tgaaaaagct gttctgcatc aagtaataag
    13681 aagagccaag taaaaacagt ctcttatgtt tttaacttgg ctggacttat caccgtagat
    13741 gtaagctttt gaacgccgtt tatttgcaac aaaaggggct gtgacacgat ttttgtcaca
    13801 gccctttttt tgcgaacatt catttagcaa tttttagaaa cttctgccgc caccgccaaa
    13861 agtcccgcca ccgctagaat gggtagtact tccgccaccg ccgcttccag gaggtgggct
    13921 tttggggatt cttcttgtcg tgacaaaaga atttgtcaga cgatcggttt tttcagttaa
    13981 tttgatactg gatttttctc ggaatggata tttgtagatg cctgatttca attgataacg
    14041 cgaaacagtc acaaaataaa agacaagact aaggataaga gccaaaacaa cagcaatgat
    14101 gatttctact gtcgtcaaaa ccttatagcg tgtaattttg ccagtttctt cgtctacacg
    14161 ataatgtcct cctggaactc cgtcttccac atatgttgct gatttagaca ggtaagcttt
    14221 tgcagcattg taataggcag catttgtcat ctgatcataa acatcatcta agatcgaatc
    14281 gattctgcta tcagtcaaat aatcgatcat attaccagaa gtcgaaatgt agatttcacg
    14341 ctgtcccata tctaatagca atactgagcc attttgatca tttccgactg cgtctcttaa
    14401 gtaattatca gcaaagtctc ttggttcttc tgaattagat gtagtcgtta cgatcaagac
    14461 tcgtcctttg attttttcat tcagcttttc tgcctgttgt tccaaagatt ggatttgatc
    14521 tgttgtgaat aacccagcct catcatttac cgtagctgta tctgcatgag tagccaccgg
    14581 ccagccaaca cttactagca aaagaaagaa taaaaaaaat tttttcatca tatcaagtaa
    14641 cctccgatca gaaagaggat cgctagccca gcgaaaatcc caagagcggt cagtcccaat
    14701 tttttctggc tgatcggcag gacaccgctg acctttcctg tttgtccatt cattgcgtag
    14761 tagtagactt ttttacttga gtcattgctt cgataagtaa caagccatac cggcagtaat
    14821 acgtaattat ttacttcgtt tgtgattgaa gcagaagttc gcacaccggt caatgttgta
    14881 tatccatttg cggtatcttt taacagcttc tctgaatagt cttttaattc agattgaatc
    14941 tgtgatttga ttgcctcata ttcgatatct cttttttctg cttggaaccc ggctaaatac
    15001 tggcttttaa aatcaattgc tttatctaac gggaaaggtt gcacgccttc gaccatcttt
    15061 tgttgcgtat tttttgataa ggcatttttt actagttctt taaaagaaag cttgcctgct
    15121 cttgaaacag caaattgctt cgtttctgtg tattcgatgt cacctatccg ccatatgcgg
    15181 ataactgtac cattcgcctg catggcacca tctacttcag catctgtagc ccaataaggg
    15241 aaatacacac ctgtcaactt atcgatttga tccttattga aaaaagcttt gggaacaaac
    15301 cattttttct tggtccaagc gagaaatctg ttgattgctt cttctttctc gacagcaaaa
    15361 ggaagtactt tatttggcaa gaattctccg cttaaacgtc ccgatagtac gacgggatta
    15421 tggcagtagt aacaataagt cgcagcagtt gtcgcatcag tcacgatctc tgcaccacag
    15481 ttgggacaaa gaaaaagatc tatcgctgcg gtattatctg cagattcgtc cgctgtatct
    15541 gaaaaaccgc ctgcctcgtc aaccgctttg cgctcggctt cattcatatc ggcaagctgt
    15601 tcttgggctg taaaagtaaa atcttcggta ggtgaaggtt cttctttgct ttcttcgcca
    15661 gccatacgcg cttctttttg cttttgctca tattggctga cttcttcttc tgtatagata
    15721 ttcaggcagt attcgcaatg aaatttttgg tctttgggat caaaagtcaa cggaccgccg
    15781 caattaggac atttatgtgt aagaacatcc atcagctgca cacctttcta aataaattaa
    15841 ttaacgggga ttcctttaaa cggtttacca cagtttggac agaatttagg gatgccgttg
    15901 gaaagatcca cgatctcatt gcattcactg catcgcattt gtagcttagg ctgaacgttt
    15961 tctacaggtt tggcagctcc gcagttggaa cagaattttc cggaattttc tgtcccgcaa
    16021 actggacatg tccaagtatc cgcatttcct tgtgcagttt gttgattttg tttttctgct
    16081 tgttgtttga tttgttcttg gttattttga gctgcttgag aaagataaga accatttgca
    16141 ttcattccca tacctacacc cataaagccg gtcattgctc ctgcttcatt ttttccggct
    16201 gcttccatac cacgtgcaat ggaaccttgc acgtatcctt cacgtacgct aggatcacct
    16261 agcatcgccc cttcgttacg catattgatc agtttcacag aatcatcggt gtaagaaatg
    16321 ctggctacag ccacagaaac aatctccatc ccacgtaatt cacgccaaga atcatccaat
    16381 gcagtatcca tgtatttgct taattccaaa ctcttagatg gtacataaga aatccgttga
    16441 ccatctgcag acatctgatt gattgctgat tgtagcgctg ttaggaattc agctaaatac
    16501 tgctcattga tatcattgat ttccacttgt gttttgtttt tagggattgc gtttgtataa
    16561 aataaaatcg ggtcaacgac atgaatagaa taagtgccat gtgcccgtaa gaataattct
    16621 gcgttataga aattgtcaaa gtagttcaac ggcgcgcttg taccgaattt gattcccttt
    16681 atttcttgga gattgatata aaaaacttgt tgtttctgcg gtgtgacgcc gccaaatttg
    16741 aatcggtcaa aagtttcagc aattgcttct ttcagtgttc cattgaacat ggaaggagct
    16801 gcgtcatttt taatggtgta ataaccttcc tcagcggtat aatcgatgat ttttccacca
    16861 tcgaccaaaa gcatcatcat gtttggatag acatggacga ctgttccgtc tgttaaaaca
    16921 tcttctgttc ctttacgatt ggagccacgt ttgtcatctt tgcggacttt gacacctttt
    16981 gtcatgaccg ttgtatcact catattgtct ggttcgatga cttctagcca ttgatctgcc
    17041 agaccgccgc caaccatact tgttgctgct ttgataagac ccataaaaaa ttcctcctct
    17101 gattcagcta gtgccaaaca attattgttt atttattata ccataggaag atgtgaaact
    17161 tctcggacta gagacggaat tgaaatggaa aattgcgtat tgtttaaata cctgaaaaag
    17221 ccgagaagga tagtagtttt tttacctaat atcacgtata ataagagata gtgaattttt
    17281 taaaaaaggg ggatccttat ggcatatcaa gcactttatc gagtctggcg ttcgcagcgt
    17341 ttcgaagaca tcgttggaca aaaagctgtg acacagacac taaaaaatgc tattgtttcc
    17401 cacaaaacct cacatgccta cttgtttaca ggacccagag gaaccggaaa gacgagtgca
    17461 gcaaagattt tcgcaaaagc aatcaactgt ccgaacagta aagacggtga gccatgcaac
    17521 gaatgcgaaa tgtgtcgatc aattactgct ggtacgcaag aagatgtgat cgaaatcgat
    17581 gctgccagca ataacggagt cgaagaaatc cgatttatcc gtgatcgggc aaattatgcg
    17641 cctacaaagg cagcgtacaa agtgtacatt attgatgagg tacatatgct ttcaacagga
    17701 gctttcaatg ccttgttgaa aacattggaa gagccaaaag aaagtgtcat attcatctta
    17761 gcaacgaccg aacctcacaa aataccagct acgatcattt cacggactca gcgatttgat
    17821 tttaaacgga tcaatacggc agatattgtc gaacatcttg aatttatctt gaaagaatcg
    17881 actatcagct atgaaccagc tgcattgaat gtgattgccc gttctgcaga aggcgggatg
    17941 cgggatgcat tgagtattct tgatcaggca atctcgttta gtgaaggaac aattacttta
    18001 gatgatgcca tgcaagtgac aggtagctta actgacgaaa tgatggacag ttacttgagt
    18061 gcttgcgtga aaaaagaagt accagaagca ttggaaacat tgaaccaaat tctcgcagcc
    18121 ggcaaagaat cacgtcgctt tctagaggat atgttacaat actgccgtga cttgctgatg
    18181 tatcagcaag cacctaacct agttgaggaa caatcgagta cgatcagaag tacatttaca
    18241 gaattggcac aaactgtttc agcggatcag ctgttttcaa tgataaagat tttgagcgat
    18301 actcaaaatg aaattcgttt taccaatagt gccgctattt atttggaagt cgctacagtc
    18361 aaacttgcac agctaccgtc tgcttctgtt ccttcagcag atgtagcatt tgcagatgat
    18421 cagatagtaa aacagctaca acaagagatt aaggaactaa gaaacgaaat caaggaacta
    18481 cgcagtaata caggaattga gaatacaaaa gtacagccgg tacagcaaac aaatcataaa
    18541 aaacagacaa gtacctatcg tgtaccgacg gaacgagtat ataaagttct gcgggaagcg
    18601 acaagacagc atctgaacca agtaaaagaa gtatgggaag acttgctgat gagtctatct
    18661 gtcactcagc gagcaatgct taaagcaagc gaaccagttg cagctggacc tacaggacta
    18721 gtgattgcgt ttgattatga gattgtttgt caaagagcag caaatgacga agaattgcag
    18781 ctttcggtac acaacaactt aagccgaatg atcaaagatt atgcgcctga tcctgtttat
    18841 attacaagag aaagctggcc agaacttcga agaaattatt tgacgcaggc acaagaagag
    18901 ggaacagaaa actcttttgc ttctgaagac tcagaaggag aaatcgagtt gcttcctcaa
    18961 gaacaagaag taccagaaga aacacttgta gtaaaagcag aggaactgtt tggctccgaa
    19021 gctgttacag tggtagatga ttaataaaaa ggagtagata attatgatgc gtggaatggg
    19081 caatatgcaa ggcatgatga aacaagtaca aaaaatgcaa aaagaaatgg cagaagcaca
    19141 agagctgcta aataaaacag agtttacagg taaagccacc aatgaattgg tcaaagcggt
    19201 ttttacaggc gatcgtcgca tgaaagatat ccaaatcaat gaagaagtcg ttgatcctga
    19261 tgatatcgag atgcttcagg atcttgtcgt aatggcagtc aatgatgcga tcgcacaaat
    19321 tgatgtggaa actgagaaaa aattagggaa atacgcaaaa ggtttgccag gattttagtt
    19381 ttaaactgaa ggagtaatga atatgcaata tccagaacca attgcgaaac tgatcgacag
    19441 ctacatgaaa cttccaggaa tcggtcagaa aacagcgaca agactcgcct tttatacgat
    19501 cgatatgaaa gatgaagtag taaatgaatt tgctaaatct ttgttaagtg taaaacgcga
    19561 tttgcatttt tgtagtatct gcggaaacat cacagaagaa gatccctgcg aaatatgtag
    19621 aaatccttca cgtgatcgaa gcgttatcct agtggtcgaa gagccgaaag atgtaatggc
    19681 aatggaaaaa atgcgagaat atcacggact ttatcatgtt ctccatggtg tgttatcacc
    19741 gatggaaggg acaggtccag aagacatcaa tatttcttct ctgttgcagc ggctgcatga
    19801 tgcagatgta aaagaagtga ttatcgcaac aaatgcaaca acagagggag aagcaacagc
    19861 tatgtatctc tcacgtctga tcaaacccgc aggtatcaaa gtgactcgtc tagcacatgg
    19921 tctgtctgta ggctctgata tcgagtatgc agatgaagtt actttattga aagctgtcga
    19981 aggaagacgt gagttataaa tttctaatta agtgtttttt cagatacaat ataaaagcag
    20041 cacaagagat gggggcaaca atgtgaacgg actatttatt acaatcgagg ggcctgatgg
    20101 tgcaggaaaa accagcatct tgaatgaact ttaccctctt ttgaaagaag tagcaaaaac
    20161 agaaatcatt caaacaagag aaccaggagg cattccgatt gctgaagaga tccgagcagt
    20221 gatccttgat ccaaaaaatg atcggatgga tgaacgtacg gaagcactgc tttatgcagc
    20281 agcaagaaga caacatctag tcgaaaaggt tctgcctgct ctggcaaaag ggaaaatcgt
    20341 tttatgcgat agatttgtcg acagttctct tgcttatcaa ggtgcaggcc gacgcatcgg
    20401 cgttaccgag attgctcggt tgaatgagtt tgctacagaa ggtactacgc cggattttac
    20461 cctttatctt gacgtagatt cagacaccgg attaagaaga atcaagaaaa acagacaaaa
    20521 tcaaatcgac cgtttagatt cggaaggttt ggaattccac cagcgtgtac gtcatgctta
    20581 cttaaagctg gcagaagaga atccggaaag aatccacaaa gttgatgcaa gaaaaagctt
    20641 tgaagaagtg ctgcaaacaa gttatcatac aattattgaa cagtatccgc aattttttga
    20701 aaactaggaa ggtgaacgtt tatgaaaatt attttagcga ttatccaaga caaagacagc
    20761 aaccgtttat caaatgaatt gattgatgca aatatccgtg ccacaaaact atcatcgaca
    20821 ggcggcttcc ttaaggctgg aaacagtacg tttatcgtgg gtattgaaga tgaccgtgta
    20881 gaagaagcat tggaaatcat caaaaaaacg tgtgaatcaa gaaagcaatt tgtatctaca
    20941 cctgttacac tggatatttc gatggatggg ggcgtccctt atccagtaga agtagaagta
    21001 ggcggtgcca cagtcttcgt cttacctgtt gaaggattcc atcaatttta ggaggagctg
    21061 agctatgaat caagaaataa acttggctca aatgcaaccg atcgtgtatc agcagcttca
    21121 acggagtttt gagcatgggc gtatcgctca tgcttatctt tttgaagggg agaaaggaac
    21181 aggaaaacat gaagtaggta tatggcttgc acagcacctg ttctgtaccc aaatgaaaga
    21241 caatctgcct tgtggaaaat gcaacaattg tcagcggatc gaaaagaaag aacatccaga
    21301 tgttttggtt attgaaccgg aaggacaaac catcaaagtc gatcagatca gacgtttgca
    21361 gacggaattc agtcgcagcg gttacgaatc aagaaaaaaa gtttttttga tcaaagaagc
    21421 tgaaaaaatg aatagcagtg cggcaaacag tttgttgaaa ttcttggaag agccccctgg
    21481 agatttttta gcggtgttag aaacagatgc agtaggaaga attctcccta ctattcaatc
    21541 tcgttgtcaa gttctgcatt ttcaagaact ttcgaaagaa gcactgatcc atcgattgca
    21601 agaagagcag attccattgg aaaaagcaaa acttttggct ttcttaacaa acagtttagg
    21661 aaaagcagtt gaaatctctc aagatgaatg gtttaatgat gctaaggact tgatttatca
    21721 atggttcgtt tacttgcaaa agcaagatac tcaagccttt atctatgtcc aaaaaaaatt
    21781 ggtaaaatca tttaaagaaa aaacacaaca gtttacagga ttatctgtct tgatgtttta
    21841 ttatcaggaa gcacagcgtc aagcagtagc aaacggcgaa tatggcaaag tgaaacagat
    21901 aaaccagata attgaacgga tattgatcgc agagcagaaa ctacgcagca atgtcagttt
    21961 tcaggctgtt gcggaacagt tcgttttgca tacgattttt tagcttgccg caaaggatga
    22021 tgatcaagga ggatggaaat ggtagaagta gtaggtgtcc gatatagaga tgcaggtcat
    22081 atctattatt ttgcaccagg agactctgaa tatgtttata gtgacaaggt attagtcgaa
    22141 tctcagcaat ccttacatat tgctactgtt gtccttccta aaaaagagat tgatcccaat
    22201 gaccttcctg aagctatccg tccgatcttg cataaagcgt cacaagctga attagaaaaa
    22261 gacaaaaaaa acaaaaaaga tgcttatgat gcattgaaaa tcgcaaaaga aaaagtgcag
    22321 ttgcgtgagt tggaaatgaa aatggtcaaa gccgaataca catttgatcg aaataaattg
    22381 attttttctt tcacagcgga cgggcgaatt gactttagag aattggtaaa agatctggcc
    22441 gctgtatttc gtactcgtat tgagcttcga caaattggtg tccgcgatga agcaaagata
    22501 ataggcggaa ttggtccatg tgggcgtcca ttatgctgtt cttctttttt aggcgatttc
    22561 gtgcctgtat caatcaaaat ggcaaaagat caaagtttat ctttgaatcc aacaaaaatt
    22621 tccggtttat gcggacgctt gatgtgctgt ctgcaatatg agagcgaaga atacgagaca
    22681 gcgaaaaaag aactccctga ctttggaaaa gtaattgcta cacctgatgg taaagggaaa
    22741 gtcgtagggt tgaatctgct tagccgtatc gttagtattc ggctgactgg acgggaaaat
    22801 cctgtcgaat atgaatggga tgaattgaaa gaatttatcc aagtaggtga gatgaatgga
    22861 taagagatca ttatacgacg gattgaatca attagaatca gagttgagaa atacattaac
    22921 agaacttgtt gaaatgaagc atgctttgca tgagatcgta gaaaaaaata caactttgga
    22981 gatggaaaat caacgtctgc gggaacacct gcgggaattg aaccaagttg cacaagctcc
    23041 agctgaaaat gtgaagcagg aactttctaa gtctcgtatg aatcttgaaa aaatttatga
    23101 agaaggattc catgtctgct acgatttata tggatcaaga agggacaatg acgagccttg
    23161 tgcgttttgt ctagaagtga tctatcgcga aagcggtcga tgaccaataa caagaggaga
    23221 ctggagcgga gaaatactgc tccagtcttt ttattaagga gattgtgaca gatgtataat
    23281 caaaaaagtt tcaagggaaa tccggtatat ggggatttat atcttgtacc gacgccaatt
    23341 ggaaatttag aagatatgag ctacagaagt gttcgtatgc tgcaagaatc cgatcttatc
    23401 gcaagtgagg atacgcgtaa tacacaaaag ttattgaatc attttgaaat ccatacaccc
    23461 caaaaaagtt tacatgaaca taattataaa gaacgtgtcc ctcaattgat tgctttgtta
    23521 aaagaaggaa aaacgattgc tcaagtcagt gacgcaggaa tgccttccat cagcgatcct
    23581 ggacatgagc tagttttagc ctgcataaaa gaagggattt ctgtgatctc tatcccagga
    23641 cctacagctg ggatgacggc tttgattgct tcagggctag tgcctcaacc atttttattt
    23701 tatggcttcc ttccacgtaa gaaaaaagaa cagaaagaaa cattgaaaaa gctaaaagaa
    23761 cagacagtta cgctgatttt ttatgagtca ccttatcgga tcactgctac attgacgaat
    23821 ttcttagaaa tttttggaaa tcgccaagtc gttttgtgtc gggaattgac aaaggtccat
    23881 gaagaatacc ttagaggaag tacggaagag ctactcgctt atatcgaaga acatccagta
    23941 aagggtgaat gttgtctgct tgtagaaggg aatttgttcg aaatgccagt gacggaagtt
    24001 gatgaagagt tgggaacgtt aaaggaacaa gtagaagcaa agatcgcttc aggcgaaaaa
    24061 ccaaatgctg ctatcaaagc agtagcgctg aaaaatgggc tgaaaaaaca agaagtctac
    24121 cgtcagtacc atgaattaga ctgagatagt ataagttgtc tataatgaaa gactaatgag
    24181 gaaatcagct actaactttt caaatctctt gaacgcgaac gaatgttctt gtataatgaa
    24241 taagaggtga ggatgatgga gctgcaattt cctttacata atgatacatc aagaaaaatc
    24301 attcatattg atatggatgc ttttttcgct tcggttgaag aacgagatca tcctgagttt
    24361 cgcggacatc cattagtaat tgccagacac cccagtgaca ctggtgggcg tggagtagtg
    24421 actacagcaa attatgaagc aagaaaattc ggaattcatt cagcaatgag tgcacaaaaa
    24481 gcgtatgagc tttgtccgca agccatcttt aagcctggcg accatcataa atacagcgat
    24541 atttccaaac aagtacggga agtattctat cgttatacag atattgtgga accagttagt
    24601 atcgatgaag cgtatcttga tgtgaccgaa aataaaatca attgcaagtc agccatcaaa
    24661 atcgcccgaa tgatccaagc agatatttgg gagagtgtcc agctaacatg ttctgcggga
    24721 gtaagttaca ataagttttt ggccaaatta gcatcagact atcaaaaacc tagaggaatc
    24781 acggtagttg cccctcaaga cgctgtgcca tttttaaaag ctttgccaat tgaaaaattt
    24841 catggggtag ggaagaaaac tgtacctaga atgcatgagc tagggattta tacaggaaaa
    24901 gacctatatg aatgcacaga aatgatgttg attcgaaatt ttggaaaaat gggctattct
    24961 ctttatcgta aagtcagagg aatccatgat tctccagtaa acgttacaag agagcgaaaa
    25021 tctgtgggaa aagagcatac ttatgggcag ccgttgcaaa cggaagaagc agtgctgact
    25081 cagttgcgcc aactagctga aaaagtagaa gaagcactga gaagagtcca aaaacatggg
    25141 aaaacagtag ttttgaaggt caggtacacg gactattcta ctgtgaccaa aagagtgaca
    25201 ttgccagaat atatctataa aaaagaagca ctcttttatc aagcaagcct tatatgggag
    25261 gaaatcttgg gagtagaaaa aggaatccga ttgttaggga ttactttgac gaatctggac
    25321 ccgatgactt atgaaaacat cgtccttcca ttatgggaaa atcaagaaat ataaaaacag
    25381 ctttgagagt tgaaaccgga aatccgtttt ctctcccaaa gctgttttta tatcaagtct
    25441 tgctttttga tttgttcata ggtttctgtt gcatccgtac atttttccca aatcacaact
    25501 tgattggatt ctaaagattt ttggacgtca atgatccgct ggttgctact tccgcgaaat
    25561 tgtagtttta ggtcacgttt gcttaattca aagcgtccgt caacaaggat atcgatttgc
    25621 gaaaggagtt ctaatttatc ttctgaatct tgtagcaatt catcaaaggt atagcctgtc
    25681 catgaccaga tatcttttgt cgtaccgaac tcttttcgga tacgtcgtac gacttgcaga
    25741 caaacatctg tattcaaaaa aggttcgcca ccaagtaaag ttaatccttg cacgtaatcg
    25801 ttgcgcaagt cagcgatgat ttgttcttct aattcaggtg tgaatggttt gccatatcgg
    25861 aaattttgtg cagctttgtt gaagcagcct tcgcaggcaa ataagcagcc actaacgtaa
    25921 agactgttgc ggacaccttc accatcgaca aaattaaacg gtttgtagtc agcaatatag
    25981 ttttggctgt agtcttttgc taaccattct tttggttttg gatttctcat aaaaaaactt
    26041 ctttcattta aggttcaaag atatagctat tgaagataaa atctactcga ccacataaat
    26101 aagaacaaac tgacctaaat ccattgttca agaaagcttg ttcttatttt gatagagggc
    26161 gttcaaagat acaaagccat cgaaagaggg tggtgaaaaa agacttgtca tagcctctta
    26221 ttctgaataa acggtgttca aaagcttgca tctgcagtaa taagcccaac taaacgaaaa
    26281 atatgaggaa ctatttttgt ttagctattc ttattacttg atgcaggaca gctttttcac
    26341 aacctcattt catgtgtttc acacgtgagg aaatttcttt atggcggcca tgtaccatcg
    26401 gacgagcttg cggatttcct aaatagccac aagtacgttt gactacatca catgtttttg
    26461 gatcatgatt tccacattct ggacattcga accctcgttc tgtcggtttg aaatcacctt
    26521 cgaaaccaca ttgatagcaa tgatcgattg gcgtattcgt accaagatac ccgattttgt
    26581 catatgcaaa atcccataca gcttctaaag ctttcggatt ttgttggagc atagggtatt
    26641 cacagtagtg gatgaatcca cctgaacaat attttggata atccttctca aattccaatt
    26701 tttcaaatgg cgttgggttt ttacgcacat catagtggaa actgtttgta taatattctt
    26761 tatctgtgat attttcgatg atgccgaatt tttctgtatc caaacggcag aaacggtcag
    26821 ttagactttc acttggagtt gaatagacac tgaaatgata accatattca tccccccatg
    26881 catctgcatt agcttttaat tctttgacga tatcaagtgt gaaagctttt gcttctgggt
    26941 ttgtttccca ttcgccaccg aagaaagctg aagcaacttc atacaatcca atgtagccta
    27001 acgatacagt agctcgacgg tttttgaata attcattgac agaatcggta cgtgaaagac
    27061 gtttcccgaa agcaccatac atatataaaa ttggcgcatt tgctggaatg gcttctttac
    27121 aacgttctac acgatagaga agcgcatctt tcatgatttc taagcggtca gacagtaact
    27181 gccagaattt attctgatct ccttttgatt cgatcgcgat tcttggcaaa ttcaatgtaa
    27241 caacacctaa attcattcgt ccgacattta cttcttgacc attttcgtct ttccaccctt
    27301 gcaagaatga acgacagccc ataggtactt tgaaacttcc agttaattca acgattttat
    27361 cgtaattcag aatatcagga tacattcttt tcgttgcaca ttccaatgct agttgtttga
    27421 tgtcgtaatt aggatcttca gggtttaagt tcacaccttt tttcaaagtg aagatcagtt
    27481 tagggaagat agctgtccgc ttttcactcc ctaatccatt gattcgaatc tgtaaaattg
    27541 ctttttgaat ttctctttca aaccagtttt ctcctagtcc aaatcctaat gaagtgaaag
    27601 gagtttgtcc atttgaagta aataaggtat tgatttcata ttccaaactt tgcatagcat
    27661 cgaagatatc ttttttggtt tttgcttttg catattcttt ttggcgttct ggcgaatcga
    27721 tccattcttc cgcgtctttt agatgttttt tatagttcaa ttctgcaaaa ggagcaagca
    27781 attcatctgt tcggtcggca gaacagccac catattgact tgacgctaca ttggcaataa
    27841 tctgtgagat ttgtgctgtg gcggtctgga tagattttgg cgattcgact tcggcattgc
    27901 cgattttgaa cccattgttc agcatgcctt tgaaatcaat caagcagcaa ttcgtcatcg
    27961 gtgtataagg atgataatcc aaatcatggt aatggatatc gcctttttga tgcgcatttg
    28021 ctatatgtgg tggcaacatt ttcagtccga ttgattttcc aacgatcccg gctgtcagat
    28081 ctcgctgtgt attgaaaaca ttactgtctt tatttgcgtt ttcgttcacg acagtttggt
    28141 ccttgttgat tagcttttca atcgaaaagt tgatatctgt tgctttactg cgagcaaaat
    28201 ctcgtttggt acgataattg atgtactctt ctgctaattc atattcgttt ttttctagta
    28261 atatatgttc cacgatgttt tgaatctcat agattttgac attttcagca aaccggcttt
    28321 tgatttcttg gacaaccaag tctgcaatca tttgaatttt ttcataagta agtggtgata
    28381 atgagccgtg aattttcttt tctgctttga tcagtgcttc attgatcttt tgttcatcga
    28441 aatcaacatg gcggccatcg cgtttgatga ctttcatctg ggatgtctct ggctgctgag
    28501 taacgacagt tttttcttca taatttactt tcattatctt caactcctgc taaattgttc
    28561 ttgtctatca taaaacaaaa tcggggaagg agcaactaaa tatcggctat atatagtatt
    28621 caatgtgctg tataattgga tttctatact atatgtagtg tatattttat tgtgaaaaaa
    28681 ggtgaaaact tgtagctggt ggatttgttt tttgcatatt taattttgct gaataaaata
    28741 attgaataaa attttttatt cagtccaagt aaaagtgaca aaaacataag agcaaattat
    28801 tcgtagatat gctactatgc aaataaatag aaatgaaaaa tgaaagagga ggagaagaat
    28861 tggaaaagat gatcgaagtg aaacatctaa gtaaaagcta cggcagcaaa ctttctcccg
    28921 tcaaagcctt agataatatc tcattcacag tagaagaagg cgagtttgtt gggattatgg
    28981 gcccaagtgg tgcaggaaag acaacgctga tgaatatctt gtccacgatc aatttgccta
    29041 cgatgggcag tgtctcgatc caaggaaaag aaatcacaaa aatgaaaaac agtgagctca
    29101 gtgactttcg tcgaaaaaaa ctaggattca tttttcaaga gttcaatttg atcgataccc
    29161 taaatgcaaa agacaatatc ctattgcccc tagcagttga acggatgact aaagaggaaa
    29221 tggaaaagag agtaagacat gtagctcaat tgttgaatat agaagaactg ctcaaacggt
    29281 atcccgatga actttctgtc ggacagagac aacgaattgc agctgctaga gcacttgttg
    29341 ttcagccaca agtgattttt gcagatgaac caactggctc tttggactcg aagtcggcta
    29401 cagaattgtt gaactacttg aagaccatga atcaaaaaga aaaagcaacg atactgttag
    29461 tcacccatga tccatataca gcaagctact gcgatagaat tttatttatt aaagatggtg
    29521 tgattttttc tgaagttgtc agaagaggga caagaagaga gttctttgag aaagtaatag
    29581 acatgcaggc gacgattggc ggaggaggaa aaatgaatgc tgtataaact agccgttaaa
    29641 agtttttttc ggcagtcacg aggctatctt gtttattttt tcagtttgac tttatccgta
    29701 atgatctatt attccttcag cgcaatgacc tacgatcaat ctttagtcag aagagcaagt
    29761 caagatactt cgatagatgg tggattgagt tttggtggat ttatcattac tgttgttttg
    29821 ctttttttcg tattcagtgc caaccgattt ttcttgaata gaaggcaaaa ggaaataggc
    29881 atctatcagc tttttggtat gaataaactg cagatatcag gtatatatgt gttagaaatc
    29941 atgataattg gattgttcgc ctgtatttct gggatattac tcggtattat tttttccaaa
    30001 ctcttttcaa tgattttggt tcgaatgatg gatatggatc ttactagtcc attttttatc
    30061 tccattccat cagtagtaga tactttattt gcttttttca tcatcctttt agcagtatct
    30121 gtatattcaa tatggaaaat atggcgctat ccaatgattc gaagttttgg agaaaaagaa
    30181 caagcagata gttcaacaat gcgcttcagg acaaggcacc gattactcgc agtcatcggg
    30241 ttactacttt taacgagcgg atattttgga gctagtcatt ttagggaaat cacttcatgg
    30301 atgatcagtt atcattatct tgatacatta gttttctttt ttcctttttt aatttttttc
    30361 gtttgtgtgg tcggtactta tttgtttttc tgttatacat ttcggttatt tttgatcata
    30421 ttgagtaaat ggaagattta ttactatggt atcaattttt tgataatggg taacacgcaa
    30481 gttcatttgc tcaaaagttg gcgtacaaat tctctgatta cagtcattct cggaatttct
    30541 ttaatgacaa ttggcggcac ggctagtatc tctactattt tatcctacca ggagaaagtt
    30601 tcctccccga tggactatca gtttgatgtt gagacagaga aaaaaatcaa gccgattcta
    30661 gcagaggaag ggcagaagat aactaaagaa atcaccttgc attataaagt cattgggagt
    30721 atctatagtc aaaatctcga agaacctatg tttcaaactg ctaatctgat cacggaaaca
    30781 caatatcaag ccttcagaaa agttaatcca gatttgccga acatttcttt gaaatcacct
    30841 ggtcatactg ttttgcttga tcaaatgcaa actctttttc ggaatatttc attatatgga
    30901 tctagtattg gactgccaga taatcaagaa ttgaagattc aacaaatgct tccaagtgta
    30961 ttaggtgacg aaacaatggt atatctaagt cctgtactga ttgtagatga taccgttttt
    31021 gaaaaagcaa aaggtgtgga ttatcaagta gtcaatgtag atgtatcagg tggaaataat
    31081 gaaaaaatag atgaacgact gagcaatgac ctgtcgataa agtggctcaa tgctgtttat
    31141 tattcttatg atatcgtaaa tggaagtcta caaggagaaa ttagcacaaa gaaacttccg
    31201 gatgaccaaa tgaaggaagc acaatttagc caagaaagct ctcgtttgaa tttctcttcc
    31261 agctattcga aattgcgtgc agttaatcgg cgaatcggta tcaatatttt tgtcacacta
    31321 tttgtcggga tgatcgtgat tattgctact ggtagtattt tgacagtccg gcaattagca
    31381 gaagcagagg aagaaaaaga aaattatcac ctattgacta agctggggat tccacaaaga
    31441 aggatcagac ggatgatctt cgagcaaaac gcacttacct tctttccacc catgatatta
    31501 gggatcactc acgcagtatt tgcaatcaat gtcttcactc agtatataaa aactgccgat
    31561 tactggttgg cttatcttgt gagtggctta ttgatcctta tctatctttt gctttatgtc
    31621 attacgagtc agttatattg tcggattatc gaggaatgat atacggaata agacaagata
    31681 ataaatagaa ctaaattttg aataccattt atttggaata agaggttgtg acacgatttt
    31741 tgtcacaacc tctttatttt ttatattttt taaaagtacg agaaattata aagaaaaaag
    31801 cagtgatttg atgtgctatt tttataaaag ctatgtgaca aaactgttag taaaaaaacg
    31861 gtttctgtaa gctagacaca tgtttctttt tgtcaaaatt agttacactt tgtgtgaggt
    31921 tatatgagga gggaatccaa tgaacaaatg gcgttctaat ccaaaattga attattgggg
    31981 aaaattcatc attcgaacaa ttttttatag tgcagtactg ctgttcctga tttatctgta
    32041 tcactataaa aatattcaag gaggaacttt tatctataac gaattttagg aggaagagta
    32101 tggaaatcaa aacgattatt gaagcaatcg atgattgggg aataaatgaa cctgatcgag
    32161 ttgcttatca ggcggatgag acgcatactt ttggtgaatt gaaacaagca tcagacgcac
    32221 ttgcttatta tttagaaaaa aaggtagaag gcgatggacc aattgtcgtt tttggcaatt
    32281 tggagtttga aatgattgtc tcatttctag gggttgtcaa atctggccat gcctatttgc
    32341 cgattgaaga gcatacaccc aaggagcgta tcctatcgat ctttcgggtg gctaaaccat
    32401 ctatggtcat cagtatcggg gactggatag aagatattcc ttcggttcct gtcataacaa
    32461 aaaaagaatt tcaagaaatc aagaagatac cagtaggatt ttatcccaga aatagcgtaa
    32521 aaggtacaga aaatttttat attattttta cttctggtac tactggagaa ccaaaaggcg
    32581 tacaaatcag tcatgataat ctagtcagct ttgttcaatg gacgatggaa gactttggga
    32641 tcaagcctgg tatgcatttc ttagcccaag cgccattttc ttttgattta tcggtatttt
    32701 ctatttatcc tgcattagtt tctggcggaa tgctgaagcc gttaaataaa gcagttgttc
    32761 aagactttcg tcaattattc gcgacattgc cagccttgaa gttgaatgtg tgggtttcta
    32821 ctccatcatt tatggatatt tgcttgatgg aacctacatt caatggagaa aatgtgccgg
    32881 aattggacat gtttttattt tgcggagaag aattgacgaa aaaaactgca gaaagtctac
    32941 ttgatcgatt cccaaatgca cggatatata atacttacgg tccaacagaa gcaactgtag
    33001 ctatttcaag tattcagatt gaccagcatg tactagatag ttatgaccga ctaccgattg
    33061 gctacgtaaa aaaagacacg aaagtttcta ttgtccaaga aggtaaacct gtttcaaaag
    33121 gagaaacggg tgaaatcatt attgcaggac caagcgtatc aaaaggttac ctgaacaatc
    33181 ccgaaaagac agctgctgcg ttcttttgtc aaaatgactt agcaagttac catacagggg
    33241 atgcaggtag actagatgaa tatgggcttt tattttatga aggacgtatg gatcagcagg
    33301 tcaaattaca tggataccgg attgaactcg gggatatcga acattatctg ctccaagaca
    33361 accgaatcaa acaagctgtc gtcgtaccaa aataccaagg aacaaaagta cagcagctgg
    33421 ttgcttttgt cgtattagaa aacaaaacga aacagccgga ttttcagttg actaaatcta
    33481 tcaaagaaca attactagaa gttgtaatgg attatatgat tcctcagaag ttccattatg
    33541 tcgatctgct gcctcagaca gttaatggaa aaatcgatcg aaaaaaatta acagctgagg
    33601 tgaatcctga atgatgttac ctcatatgat cccttatgca gagccgtttt atttttttct
    33661 tttagccatt gcgcttgtac cgatcgttct atctttattg atatgggaaa aaagattacc
    33721 agtttatcaa tcagcagtga cacttttctt tttatttgtc agctttggag gtaattattg
    33781 gcaacaggga gcggcgctga ttgggtatgt tttgtggcag acgatacttg tatggggcta
    33841 ttttgtttat cggcaaagga aaaatcagac aggatggttt tttgccgccg ttattttagc
    33901 gataattcca ctgtttttaa cgaaagtgac tccttttttt gaacatggaa aagcatcgtt
    33961 acttggtttt ttaggaatct cttatttaac ctttaaatct gtacaaatga tcatggaaat
    34021 aagagatggc atgatcaaaa catataagcc atttcgttat atccagtttc tgctgttctt
    34081 tccaacaatc tcttctggac caatcgatcg ctataggaga tttgaaaaag atcagctcca
    34141 tccgcctgtt cgtgataaat atgttgatct tttgggaaaa ggcgtccaaa atatttttgt
    34201 cggtttttta tataaattca ttatcggtta ttattttggt caagtactgc ttcctgtagt
    34261 gggacgcatt gccttacagc atggtggtat ttcatgggca ttagtaggtt atatgtacgt
    34321 gtacagtatg tatttgtttt ttgattttgc tggctatagc ttatttgcag tcggtacaag
    34381 ctacatgatg ggatatcaga caccaatcaa tttcaataaa ccttttttat catggaatat
    34441 caaagaattc tggaatcgtt ggcatatgac gctgtccttt tggttccgtg attatgtata
    34501 tatgcgattg atgttcttca tgatgaagaa aaaatttttt aaaagcaaga tcgtaatgtc
    34561 aaatatcggt tattttgctt tgtttcttct aatgggtata tggcatggat tgacgtggta
    34621 ctacatcgta tatggactgt atcatgcttg tctgatctgt ctgacagatg catggatccg
    34681 atttaagaag aagcataaag agcagttgcc ttctaacaaa tttacgcatg gcttagccgt
    34741 atttttgaca tttcaagcag tatgttttag cttcctgatt ttttcaggat ttttagataa
    34801 actttttttt agctaacgaa tagtgacata ttaatttata taacaaagga gaaaaggaaa
    34861 atggaagaac aaattttatc aatcttagaa gaaataacag gaacagatga agtaagaaaa
    34921 aatcgtgata ttgatctatt tgatgaaggg ttgcttgatt ctttagcatc tgttcaatta
    34981 ttagtcgagc tagacggaga gctggatatc caagtccctg tatctgaatt cgagcgagag
    35041 gattggagca caccgaataa aatcattcaa caagcaactg cattgaaggg ttgagtatga
    35101 aaaagaaact ttttgctatt ttcggcccgg ttgttttagc tgttggctta ctgtttgtgt
    35161 tttttacctc ctcctatcaa gtagatatct ctaatccaga aacgattaga cgtgcctctt
    35221 cttccatgtc agtgaacgtg ttgaaaggag atgcgataaa aaattatgca ttatctgaaa
    35281 aacaatatat tccttttttt ggctcaagtg aattaagcag aatcagtcca tttcatccgt
    35341 ctgttctggc agaaaaatac cagcgaaact atcgtccatt tttgctggga gcacccggga
    35401 cacaatcgtt aagccaatat atgatgatgc gttctgcagg agatgctatg aaaaacaaga
    35461 aagtcgtctt tattatttct cctcagtggt tcgttaaaaa tggtgtaaaa actgattact
    35521 ttaatacgta ttattctgaa cttcaaacat acgactggtt gttttcaatg aagaaagtaa
    35581 cgcctgctga tcgctattta gctagaagat tattaacttt ctcaaaagta aaagagaatg
    35641 atacgttgac ggcgattctg caaacgatca aaaaagggaa gctaccttta cctgaatctt
    35701 tgaatcagct gcgcagccaa tggaacatgc tgaagagaga ggatgaagta ttcagcaata
    35761 ttgggttgaa agatcgacag caaaaaatcg atcatgagtc aaaacgatta ccaaaacaat
    35821 accaagaaac agaattatcc attttagcaa atcaaattgg agaacgagaa acgacgaata
    35881 atccattcgg gttgaaaaac gatttttata cgcatcgtat ccgagcccac gagccagagc
    35941 tcaaacagtc acaaaaaaat tgggattatc gtttttcgcc agaattttct gattttcagc
    36001 tggtcttgga tcaattagca aaaaaccata acgaagtatt gttcatcatc ccaccggtta
    36061 acgagaaatg gagcgattat accggactat ctcaagaaat gttacaagga tttgccaaaa
    36121 agatcaaatt tcaattaaat agccaaggat tcaatcgaat agctgatttt gtcaatcaag
    36181 cgggtaccaa ctattttatg gaagacacga tccatctagg ctggaaaggc tggttggctg
    36241 cagatcaaca gatcagaccg tttttagaag aaaatcatat tactgctagt aagtatcatc
    36301 ttgacgatgc tttttttagc aaatcttggc aacatcaaat accagataaa ctacaattaa
    36361 aataaagaaa acaggcgagt gttgctcgct tgttttcttt atttttgaat attctgaata
    36421 aaagataaaa ataccaagtt tgcctgaaca aaaatcataa gcaatggtat aatcacaata
    36481 tgaatataga aatgggggaa aacatttgga tacaaaagaa gaacagctac ttattgattt
    36541 aacttctgca aaaggagtac ctggaaatga agaagaggta agagaagtat tcagagaata
    36601 tgccaaacca ttcgctgatg atatttttta tgacggttta ggcagtgtga ttgccaaaca
    36661 tggtgctggc ggaggcccca aggtctttat ttctggacat atggacgaag ttggttttat
    36721 ggtaacaaaa ataaccgaaa aaggattctt ggaattccaa actcttggag gttggtgggg
    36781 gcaagtcatg cttgctcagc aagttgaaat caaaacacgt gaagggaaaa tcgtccacgg
    36841 tgtgattggt tccaaaccac cacatgtttt aacacctcaa gtaagaaata aaccttatga
    36901 aataaaggat atgttcattg atattggcgc atcaagccaa gaagaagcca aagagtgggg
    36961 catacgtcct ggagatatgg tcactccata tattgaatac aaacgaatga atggcagtaa
    37021 atatttactg gcaaaagcat gggataatcg tatcgggaca gctgtttctc tgcgagtatt
    37081 agagaatcta tctaaagaag cacacccaaa tgttttattc gcaggaagcg atgtgcaaga
    37141 agaagtaggc ttgcgaggtg ctcgaacaag tacacacctt gtaaatcccg atattgcttt
    37201 tgctttagat actggtacag caggagacac tccaggaatg acaccaaaag aagcagattc
    37261 gattttagga aaaggcccac agattttgat ttttgatgca tctatgatcc cgcataaaaa
    37321 attattgaat tttgttattg atgtagcaga ggaaatggat attccattcc aatatacagt
    37381 gatttctggc ggaggaactg atgcaggaca aatgcatttg acaagagatg gtgttccgtc
    37441 tgtagccatt accgttcctg ttcgttatct gcattcccat acatcagtga ttcatgaaga
    37501 tgattatttg aatacagtga aactagtcac agaattagtg aaacggcttg atagtgaaac
    37561 agttgaaaaa attcggagtt atgaataatg aataatagaa tacctatttc tgaatatcgg
    37621 aaaaaagcac gagtttcttt ggaaaatcaa tggggaatca atacttggct tgttttctta
    37681 tctataatat ttacgagcgt catccagaac ttttttgacg gtttcagtcc ttttccggaa
    37741 gaaagcatgc agcagcactt attgagtttt ctagtcgata atttactttt attcgcattc
    37801 aactatgcat tgttttacat tgcgctagta gttgtacggg gtggcagacc aaaaagtaac
    37861 cttttatttt cagttttcca aagaaagtat tttggttcga tacttggcat caatttgatc
    37921 aattcagtca tcaactggct actcaacagt ttaatctttc tgcccatgtt tttaatcagc
    37981 ggagtgagta cttatatgca gctgcttttc agttcaaatg cggtacctac gggtgtcatg
    38041 ccagaagatt ttcttgatat cagctttgtt ttatcgatga tagggatgat ttttgtttca
    38101 gcactcatca tgagtattgt tggcggttta ttccaatttg ctgcatggac aaagttcgac
    38161 tatcctgatt tgtcggttat tcaatgtttg aaatatggct ggtttttatt gaaagataga
    38221 attggtacgt atattttgtt gcagctgtct tttatcggct ggtatttgct aggagcaatc
    38281 gctttgttca tcggtctttt gtgggtcatt gtttatgtga atgtgtcagt tgcagaattt
    38341 tatgaacaag cacggattga aaaaggaggt ccagacgcct attttactta gcatgtaagc
    38401 agtttattca tcaaaaaaat aacttttgtg gtaggataaa tctttgaggg ggaatgtacg
    38461 atgaaattaa ctgttttagg atgcttagga gcgtatcctt ataaaaatca agggacaaca
    38521 ggttatttgc ttcaatctga cgggttcaat ttgttgatcg atgcaggtag tgctacgctg
    38581 atcaaattac aagaacacct tgatccactt gatctagatg ctgtgatcct tagccattat
    38641 catcatgatc atattgctga ccttggtgta ttacagtatt actggcaact tcatcctaag
    38701 agaaatccgg agacattgct ttcaatttat ggacacacaa aagattccat tcactttgaa
    38761 gaattgacgt tggaaaatgt ctcaaaagga catccttatt ttgaagctga agaattaaaa
    38821 attggtcctt ttctgattac ttttatggaa acaatccatc cagtagtatg ttacgccatg
    38881 cgtatcgtag aagaaacaac ggggaaagta ttcgtattta caggtgattc aggttatttg
    38941 gaaagtttcg ctgattttgc gaaagatgct gatttgtttc tagcagatac ctatcttttt
    39001 gaaggaaatg aacatcatca tgcccatttc acttcaaaag aagctggaga acttgcgaaa
    39061 tcagctaacg taaagaaact tgtacttaca cacctacctc agtttggtga tttagaacaa
    39121 ttgaaaaatg aagcgcaaaa ggcagcaggc aaagggattg atatagaact tgctgcagtt
    39181 gacaaagtgt ttaacattta ggaggaagaa catgattttt gtaccaaatg aaaaccacga
    39241 cccaagaatc aatttagcaa ttgaacaata tttgctgcaa gaaatgcctt tagatgaacc
    39301 tattttgctc ttttatatca atgaaccttc aatcatcatc ggcagaaacc aaaatacgct
    39361 tgaagaaatc aatcgagatt atgtggaaga aaatggcatt catgttgtcc gtcgcttaag
    39421 tggaggcgga gctgtctacc atgattttgg aaacctgaac tttagtttta tcatgcctga
    39481 tgatggggat tcattccgtg actttgcgaa agtaacacag ccaatcatcc aagcacttca
    39541 tgaattgggt gttgctgggg cggaattgaa aggccgaaat gatctagtca ttgatggcat
    39601 gaaattttct ggcaacgcga tgtatgctac caacggtcgg atgtttgctc atggaacaat
    39661 tatgttcgac tctgatatca atgaagtagt caatgcatta aaagtaaaaa aagacaaaat
    39721 cgaatcaaaa ggaatcaaat cgatccgttc tcgtgtaacg aatatcaaac catttcttcc
    39781 agaagaaaag caaaaaatga caacagaaga atttcgtcaa gaaatcttgt tgaaaatttt
    39841 cggcgtagag tcagtagaag aagtaaaaac gtacgaattg actgatgaag attggaaaaa
    39901 aatcaatcga atctctgagg aatattatcg aaattgggat tggaattacg ggaagtctcc
    39961 agatttcaat ttctctcgcc aaaaacgttt ttctatcggt tcgattgaag tccatttgaa
    40021 tgtctcagaa ggaatgatca aagaagcaaa aatctttggc gatttcttcg gactaggaga
    40081 tatctctgac gtggaaaaaa aattggttgg acagaaatat gataaagcat ctctatcaaa
    40141 aatagtcagc caaattgatg tgaagaaata ttttggtgcg atagaaccag aagaattgct
    40201 taccttactt tattgaaaat aaaaaatagc ggaatccaca tatatggatt ccgctatttt
    40261 ttatacagga ttcgggtaat ttcgagagag ttgaacagtg atccgatcaa tgaaagtatc
    40321 tttttcctgc gttagtgttc ctgtgtaacg atccgctacg tctttcatca tcattaagtc
    40381 aaaacgttcc tgatcatcta attcttctgt gatgcctcga ctaaataggt ttgtatcgta
    40441 atgtgcacta tgttcgcaac aaatgacgaa atgttcctct tgttcgtgta tcgacaaata
    40501 aatttttcga tacagtggat tttcgatggc atgagtttct ctaaaggaat gctctaaagc
    40561 gtggatcaaa agttgagcca gatcgtcatc gttgatagga agttcttcgg gaagatctgc
    40621 ttggaaggtt atctgaatat tccgtttggc agctaacttc tggtaacgcg ctaagataag
    40681 atttgtcacc tggtggttgc aaaaagaaca cagcatttcg aaattttttg cttcttctaa
    40741 tatcttttcc acatagtttt tagcttttgt ttggtccgta tgcattaaat cttgcagatt
    40801 ctccatctct tggacaaatt ccaggcgaag tgtatttact tcttggatat gctgtcgaag
    40861 ctgctcataa tgttgctcca acaaatcatt tttgattttt aagaaatcta cttgtctccg
    40921 atctttgtcg gtatttaaga tgtgatcgat tagattgtag ctgataataa cgattagaat
    40981 gataatatat aaaatcgtag accaattgat cgttgtttga tagataccta atgccgtttg
    41041 gatgtaaatc aagcaatgga taatggctac caagacgatc caaggtgtac aagctacata
    41101 aaaacggtta ttcttatatg cttcgccaat gcatgcaagt gaagtgacta aagtgcttat
    41161 aacactagca atattagcaa tcggcattaa ttcaggaagt tctgcaggag taaaagttgc
    41221 ataaagcaga acgccgataa aaatcaaaag ttggaacgta caaaaaattc cgtaccattt
    41281 tttatagtaa aacattcggc tccaatagta gcagatgatg aaagctgacg ttaagataat
    41341 aaataaatga gataaaaaag aatgaacgaa tggttcaaaa agaataccac ttaaaatatc
    41401 ttctgataca gcttcaaatc ctaatgccaa agcaaagcaa cttaagagaa taagtgagta
    41461 ttcaagacgt ttttgtttat acatcaagta gatcgtatag aaaaaggtcc ctatagcgat
    41521 aaaaacagcg attatcatcg tcaatacttg cggcactgat tgtgaataaa taaaactaat
    41581 caatggtcca gttgttccta gataaacttt cggtggcagt ccagcatagt tctgataagg
    41641 agatgagact gtaagggtca atttttttcc gatataattt gctggtaaat ctatcacagc
    41701 caatgagagt ccgggattgc tttcttcctg ataatctttc cgttggtata aaatcttttc
    41761 gcctagatat acagtaatcc attgatggtt tcctttgatt agtaaatttg cttcgtccaa
    41821 cggtttttca agtgtcgttt ccataatgaa tgtttcgtct ttatccacgg taggaatcgt
    41881 acgagtgtac ttgctgtcaa acttaaaatc aggatcatct tttgtataga aaacccacga
    41941 atcttttatt tctttcattt gattgctgtc cgcaggagaa aaaataaaat aaaagaaata
    42001 agcaattcct gttaa
//
LOCUS       DAJSMI010000007        34847 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJSMI010000007 DAJSMI010000000
VERSION     DAJSMI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.007"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(6..1574)
                     /locus_tag="ON430_000332"
     CDS             complement(6..1574)
                     /locus_tag="ON430_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR3218282.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1578..1988)
                     /locus_tag="ON430_000333"
     CDS             complement(1578..1988)
                     /locus_tag="ON430_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR3218283.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(1985..2908)
                     /locus_tag="ON430_000334"
     CDS             complement(1985..2908)
                     /locus_tag="ON430_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCR3218284.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2905..3783)
                     /locus_tag="ON430_000335"
     CDS             complement(2905..3783)
                     /locus_tag="ON430_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR3218285.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3770..5107)
                     /locus_tag="ON430_000336"
     CDS             complement(3770..5107)
                     /locus_tag="ON430_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR3218286.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5110..6213)
                     /locus_tag="ON430_000337"
     CDS             complement(5110..6213)
                     /locus_tag="ON430_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3218287.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6287..7795)
                     /locus_tag="ON430_000338"
     CDS             complement(6287..7795)
                     /locus_tag="ON430_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3218288.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7761..8453)
                     /locus_tag="ON430_000339"
     CDS             complement(7761..8453)
                     /locus_tag="ON430_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3218289.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8475..9449)
                     /locus_tag="ON430_000340"
     CDS             complement(8475..9449)
                     /locus_tag="ON430_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3218290.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9749..10621
                     /locus_tag="ON430_000341"
     CDS             9749..10621
                     /locus_tag="ON430_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3218291.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11079..13277)
                     /locus_tag="ON430_000342"
     CDS             complement(11079..13277)
                     /locus_tag="ON430_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3218292.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13509..14501
                     /locus_tag="ON430_000343"
     CDS             13509..14501
                     /locus_tag="ON430_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3218293.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14561..15982
                     /locus_tag="ON430_000344"
     CDS             14561..15982
                     /locus_tag="ON430_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR3218294.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            15975..17474
                     /locus_tag="ON430_000345"
     CDS             15975..17474
                     /locus_tag="ON430_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR3218295.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKTGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17513..18907
                     /gene="uxaC"
                     /locus_tag="ON430_000346"
     CDS             17513..18907
                     /gene="uxaC"
                     /locus_tag="ON430_000346"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR3218296.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19066..19749
                     /locus_tag="ON430_000347"
     CDS             19066..19749
                     /locus_tag="ON430_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCR3218297.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19751..20587
                     /locus_tag="ON430_000348"
     CDS             19751..20587
                     /locus_tag="ON430_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3218298.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20598..22352
                     /locus_tag="ON430_000349"
     CDS             20598..22352
                     /locus_tag="ON430_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218299.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22628..22969)
                     /locus_tag="ON430_000350"
     CDS             complement(22628..22969)
                     /locus_tag="ON430_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3218300.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23037..25208)
                     /locus_tag="ON430_000351"
     CDS             complement(23037..25208)
                     /locus_tag="ON430_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR3218301.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25377..26276
                     /locus_tag="ON430_000352"
     CDS             25377..26276
                     /locus_tag="ON430_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3218302.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(26347..27204)
                     /locus_tag="ON430_000353"
     CDS             complement(26347..27204)
                     /locus_tag="ON430_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3218303.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27189..29891)
                     /locus_tag="ON430_000354"
     CDS             complement(27189..29891)
                     /locus_tag="ON430_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR3218304.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(29904..31193)
                     /locus_tag="ON430_000355"
     CDS             complement(29904..31193)
                     /locus_tag="ON430_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCR3218305.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31343..32389
                     /locus_tag="ON430_000356"
     CDS             31343..32389
                     /locus_tag="ON430_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3218306.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32391..34544
                     /locus_tag="ON430_000357"
     CDS             32391..34544
                     /locus_tag="ON430_000357"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCR3218307.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        10222 a         7174 c         6286 g        11165 t
ORIGIN      
        1 ttaaattaca gatgatcttt tccatcatac ttgaaaaact caaaatcagc ataacttcct
       61 ctttttttat ttagatcatg cactgctatc ccgataaaat ttccagtaaa ccctcctgag
      121 agtatacaca tgtttttttg cggacctact ttcttccatt cttcagttga tacgtctttc
      181 aaatgaaact gtgctgtctc tttctttaca gtgactttca aaaaagtctt cttttctgtg
      241 agtggaatcg tccaatcttc tatttgtgct actccgtttt ctgattctat catacgtagg
      301 cattttccct tgtcctcatt ccaagtaaca taacaataaa tataattgga ttcatttaag
      361 tacagtacca gtccagccat ctgattaaaa ttattaggca tgtattcaaa tgctgtctcc
      421 gcatcaaaag taaaatccgt ttgccgaata gctaacaaat gatgttcgaa ggtagattga
      481 agagattcac cggaaatcag acgtaaaaaa cctggtctac attttgtatc acaccaatca
      541 gcatttggca aaatgcgtct ggcattccag ttcttcttta gctctccttc aaatgtatcc
      601 ataaactgat gatctttttc ttgttgtacc tcttcatttg tctcaatcat tatttcttcc
      661 atcggagttg tctggctatc tttaagtctt aaccaatcgt cggacgtcca atacacttct
      721 tgaatagcgg tttctcttcc caaaatagcc gcaccgttta atggtctggt acaaagatat
      781 gtcatgtacc atcttcctga cggggtttgt actaagctcc catgtcctgt acattgcaat
      841 gtagaagcag gtttgtcact agctgtcagc ataggaaaac caggatcaag ctcgtaagga
      901 ccagtaatat tctttgctcg gcaaactgta actgaatgtc ctcttcctgt accgccttct
      961 gccgtcatca aataataata cccgttacga aaatacaaat gtggtgcctc cgttttcgct
     1021 aattctgtac ctgcaaatat tttatagatg ggaccatcaa gcttcttctt ctctgcatca
     1081 tattcttgta atacgatacc gctggattta ttcggtgtgt ccagacgata atcccatagt
     1141 tcattgacaa gccatttttt tcccgtttca agatcatgga acaaagaagg atcaaagccg
     1201 ttactattta aataaattgg ttcggaccaa ggacctgtga tctcagatgc agtgatcaaa
     1261 taattgtgga catctttaaa cggacggcct gcacttttga catctgtgta aacaagataa
     1321 aacaatccat cagcataact gatctgtggc gcccatatac ttccatttgt aggatttcct
     1381 tgcaaagata cttgagtggt caaaatatcc gttgtatgtg accagttgac taaatttttc
     1441 gactcatata ctcggattcc tggaagccat tcaaaagagg agacaataat gtaataccgc
     1501 tctttcactc ttaatatatt aggatctgga ttgaatcctc ttaaaatagg atttttcact
     1561 ttgatagtct gcatgattca ggctcctttt ctcattctta ctcgatataa actgaatgcc
     1621 ccagctgcga aataaaaaaa tgcaaaagga aacgcaataa actgagtgcc aatcaacaat
     1681 atgaatcctg ttataaaata caaaccagtg gcagttgtta agtattttcc tttagcagtc
     1741 aaaaacaatg ctactgctaa aaaagcaagt gaaataaaca acacaacggc aaacagacca
     1801 gtcatgtcct gtaataatcc aaatgtattt tctaaagaca aagttgaatg atctacagca
     1861 agcgactgat aacttttttt aaactctgac gcagacatat tacttaaagt aactgctaac
     1921 ccgccaaaga aaatcaaaga tagactgcca ccgaccattc ctagcaatag ttcccatttt
     1981 cttttcattt ctgaaccccc tgcttttcta aatgtatcca ttcaggtctt ttgatcgatg
     2041 tttcataatt gttattacgt tcagagaatt tgacagtctg ttcatttttc ttgtcatccc
     2101 aattatccca accagttggg tgaacatgcc cacctatctt acacgcttca aataagacat
     2161 ttgcatacgg acgccacggt cttcctaaat agacgtttct agtatcaggt tctgcagtca
     2221 aggtacagct ttcaaaacga taccctgttt tttgattttt cggcgtagat gctgctgtga
     2281 tgtatccagg atttttactg gctaagctat ggatatgaca ctgatgaaaa acggcatctg
     2341 ccccaccaaa tatgaaatcc accgttcctt caatccagca atattcatag gtctgacgac
     2401 aatactcagg attggcagaa gccggtgtac aaaaaggagt accatctttt tgcaaatcag
     2461 gcaacggacc cgtgcataag gtatcctgat aaccagatag tcgacagttt tttaaagata
     2521 cgcgatctcc tgaattgaat aaagcaacag cctgtcctac cttttctcct ggacctgctg
     2581 tattttcgat gtgaagattt tctaatacga cattcgatcc ttctaagaaa aaagtagcac
     2641 ttcggaaagt ccctaactgg ctaccgtcaa gatactcctg ttttgcaaaa cgattaccgg
     2701 agataactac tgttcctgct cccacaagtt taaaattgga taatctgctt tctatatatt
     2761 catcatatct tccttctttt actatcaaga cctttggatg gctatccggc aggcctgcca
     2821 aataatcgat tccttcttgg atagtttcaa aatccgtcgg ggtatctata gcattctttc
     2881 caattatcag tttgtctttc atcctcataa atgctccctt atgaattgtt tccaaatatt
     2941 tctcatttct tgtgtttcca tatgaccgcc tgttaaagaa aaagaagaaa agttttctgg
     3001 actacccatt gattgatact ttttgtccag cccttttctc agcttcagta cgccttcgtc
     3061 caagcacatc gtatcgtttt ttccaactaa gctaagacga gggcgcggag cgattttttc
     3121 ttggattgct aacgttgtat aagctgtcaa tagatttggc acatagtaat aaaacccatg
     3181 atgatctagg cggcgatgta gcaaaagtat ttcgatgtcc acctgtgccg ctagatcaat
     3241 acaaactttg acacgagaat ccaatgctgt cacccaccag cttaataagc ctcccataga
     3301 aagaccaatc gttgcgagcc gttgagtatc tgtgtcttcc cttgtctcca aataatcgat
     3361 caagctgatc acatcataaa tacgcattcc ccataacgtt tttcctgtta ataaaaattg
     3421 tttgaacaac tcgctttcag aaatacctcc gcgttcttca aatccccaag catcaatcga
     3481 ccaaacagca tatcccattc ctgtcagtac ttctgcaaaa gatggagaag ctaagtaagg
     3541 agcaccatgg atcaattcac ttttcccgac tgaaaaatct ccgccgtgag aatgcaggta
     3601 aaggaccggc ggaaacggtc cttctttttc tggataagca tagagggctg gtactgtttc
     3661 caattcattt aagtacaatt gatagtattc aagcataaaa ccgttttgtt tttctcggta
     3721 taatcgtttt ccactagggg tatgtctgct agatggagca cctaaaaaat cattcaactg
     3781 cataagcaaa gtttttacga acaaccagtt catccatatt ttctgattga tttgattgga
     3841 atttctcaat actgatttct ttagtatttt ctaatagaaa tgctggtccc tcgtgatttt
     3901 caatcgacat ctggttaaag gaaaccttac ccgcaaagcc aatataaaat ccttgtctgc
     3961 tcatttcctc tattcctgtc atcatagcag gaacaccggg aactgcatct tgggccatcg
     4021 agacatcgat attgtcaaat gtgatttctt gtatgaattg ttccgctaat ccataaataa
     4081 aacctgcaga agcatggaca ttccttgccg tgatattcga aaaatggatc cttctgaatg
     4141 ctggtgtccg ttcatctatc ggataagcct ttttttccca tacatacggt tctttccctc
     4201 gcggaccaca gaaataataa agattcagta taaatggaca catcacatta tccatgacta
     4261 tattggatac tcgaatatct tcaacgatcc cgcctcgacc tcgtctagat ttcaatcgta
     4321 ttcctcgatc agtttcttgg aagatacaat ttgaaatggt aatattacga atactaccac
     4381 tcatttcact tcctaatacg acaccgccat gtccatggac catcgtgcaa tttgtgatcg
     4441 tgatattttc gcaagcgatc ctttcgtacg tatcttcagt ccctgccttg attgcaatac
     4501 agtcatctcc cacgtcaata tggcaattac taatacgcac atttttgcat gattctggat
     4561 caattccgtc tgtatttggt gaatccgctg ggttcaagat cgtcaagtta tcaaacgttg
     4621 cattactgca aagaatcggg ttgatcgtcc aacttggtga ctggatcaat ttaatatctt
     4681 tcacagtaat tcgatgacag ttatggaaac tcatcaattt aggacgagga taagcgagat
     4741 tatctggttc atttctaaaa gtatgccacc atttttttcc atttccatct aatgtaccga
     4801 aaccagtgac agagatgttc tctacatttt gagcatagat acaggaagca taaacttttc
     4861 gatggactcc ttcccaacgt gaatgaacaa caggataatc ttttggatcg tcagagaatt
     4921 tcagaaccgc tcctgcgctt aaatgaagtt cgacattgct ttttaaaaat aaagctcctg
     4981 ttagaaattc acctgctgga acaactacgg tcccgcctcc gtcacttgct gcagcatcaa
     5041 tggcttgttg tattgcttca gtattcaatt catcaattga tgcgccaaat gtcaaaatat
     5101 catacatatt tataatgcct ccacttcaat caatgcttgc aagaatgcac caacgccttt
     5161 ttgatcatta caaataattg gttcactgat gtagtaagca tatgaaccat ctcgctgatc
     5221 tgctccgcct aaccctgcta cttgacagtt tttattcaag ttcagccagc cttcttttgt
     5281 ttccagaaca aattcatcaa gtaatccttg atgtgatttc ttcacttgtg gaagccattc
     5341 ttctttgttc agcagtccca aacgtattcc tttagccatt gcgtaaacaa acatactact
     5401 gccggaagct tccagataat ttcctttccg ttttgtttct gttgttactt gataccatgt
     5461 cccgctgttt tcttcttgat atggaagtaa tgcagccagt gtcttttcta gaatttcttt
     5521 cagcaaagaa caatctgttt cttcatgaag taattcatag gtatctgctg cagccatcaa
     5581 ataccagcca atcgatcggc tccaaaaatg atgggataga cctgtttttg gatcagccca
     5641 tggttggacc ttcttttcat cccatgcatg aatcagcaaa tttgtttttg gatcgaatag
     5701 atgtttataa ctgatttcaa actgtctcaa aacatcttgg taatcttttt cttcactaaa
     5761 attctggata aattcagcat aaaaaggaga acccatatat aagccatcca accaaatttg
     5821 ataaggataa atttctttat gccaaaaagc tccttcggat gtgcgtggat ggtttgccaa
     5881 ctgctgggaa aggagttctg ctgctttttt atatttttct tcgcctgttt ctttgtacaa
     5941 taaaaagaaa agctttcctg tatttacatg gtcgatattg tattcttcaa cgctgtagcc
     6001 acggatcgtt ccatcttctt gcacgaaata gtcgatattg tcttttatga aatcaaaata
     6061 aattttttct tgggttcgtt cccaaagtaa ttggaaccct ttaagtacga cgccatagtc
     6121 ataagaccat tttcctttat accacttttc ttcatataag cgaggtgttc tttccatgat
     6181 actttttgct aatttctttg accagttttc cattatttgc tctcctttaa aaattaaaat
     6241 aggatttggg acaaatacca agaagtattt gtcccaatcg tctatattac tgtgccgctg
     6301 ctttgtatgc ttcctcgtac tcttgtgtga ttcgatcgcc tccttgtgct ttccagtctt
     6361 ctacagcttt tttgaatcca tcttcgtcaa gctgtcccat gatgtattgc accgtagcat
     6421 caatgacgat tttttcaatt tctgatccta cttcattgtt cgtcggtgaa tccaatggca
     6481 cagaaggatc cattacagca aattcttcat tttctttgat caattgatta gagaattctt
     6541 tttcagggtc tgtatctttg aaagagaacg tcacttcact aggacgagag cttgataacg
     6601 gttgcacctc ttgttgccaa agatccatat cgataaattc gatagaacca tcatcattca
     6661 attcataatg cgttccttcg ataccgccag tcatcaattt atacacatct tcttctaaaa
     6721 gatcatcaac aaattgaagt aatttcttca attgtgcttc gtcttttact tgagatttgg
     6781 gaaatgctag caagccgcca ataccatttc cttctgacca aacatgataa ccgccgcctt
     6841 caccatctga gatcttgttt acaggcacta attccatatc atcttgaata ccttgaccca
     6901 tcgtcttcag attcttgata tccaccattc cagtgtagat tccagttctt ccttgtgcaa
     6961 attgttcttg ctgatcagtc ttttgggtaa ctgcaaagtc ttgtgccaaa tagccattct
     7021 caaatagatc tcttgaaaaa ttcaaagctt tcatatactc ttctgtatca aattctggag
     7081 tgaatttgcc attatcgtct acagcccagc catttggtgc tccaaaataa gaagtgaaaa
     7141 gtttaaagct ggtataacga atatcactag gcgcacgatc gccaaaacca actgtgtcgt
     7201 ccacaccatt tccatcagga tcgttttctg taaattgttt agctacttca taaagctcat
     7261 ccatggtagt tggaacatct agtcctagat tatccaacca gtcttttcga atgactaagc
     7321 cagcacgagc gtagtctttt tgtatcggta cgccgtacaa ggcaccgtcg atactcgcag
     7381 cttcgattct atcttcagaa atttgactta aatttttata gtcttttaaa tattctgata
     7441 catcccagaa tacgcctgat tttaacgcat tgcggacaga agagtttttc aacatcgcaa
     7501 tttgaagtgt aacgatatct cctagttcat cagaagccaa tgcagttgtt aatctttctt
     7561 cttttgaagc atcaggaatc cagttgaatg tcagttccgt atcggttttt tcttcgatca
     7621 agttcacaat cgtgtctgta ggcggtgaag catgatgcaa aatatttaac caagtgattt
     7681 tagcttttcc atcttctgtc tctgaagaac tgttacttga actgctgccg catgcggcaa
     7741 gtgctagaat tcctgcaaaa ctaagaccta attttacgag cgttgctttt ttcataaatt
     7801 ttctccttca ctgttttaat aatatttttg ttttcttctg ttttttctgt atatgagttc
     7861 ttccattcca aatggcaaaa tatagaattt gctgtccacg tgactgcgga aaagaagaga
     7921 ctgccaccga taaaggttaa aacacctggc attgtttgga tgatcatcag ataaatccct
     7981 accatactta atatcaacac taatgagtaa tgaagatgtg agaatccgaa taaaaatgcc
     8041 attttcattt tgaaaaaata tcctttccaa tcataatgag ccatgattgg gaaaatatag
     8101 agtgctgaga gcacatatat gaacgcagca atatacaaag cgatcctaag ataccaattg
     8161 tgtatgaaag aaagatccac aagtaaaaca atcccgatgg cgccatatat ccagctcatc
     8221 agcaaggttt cacgatagtt ttctttaaaa tatgtgaagt acgttttcgt caaaggaaac
     8281 gtttcttttg agcgcagcca ttttctaatc actgcaacca tcgcatacga tgcaggaccg
     8341 aaagtaaaaa ctcccaaacc tgcgatgact aaaccgcccc acagaaaatt caaataaacc
     8401 aactgtaaaa tacgatttag ccttccgttt attttatcta atgactgcag catatctttc
     8461 cctctctttc tatcttagta aacgccgtct tctcctagct ttttggcaat cttattagct
     8521 aaaattacca taaacaatcc tacgacccct ttaaacaatc ccattgctgt actgaaactt
     8581 aactgtccat tcttcaatcc agctgtataa atataggtgt cgaaaatttc tgcaacgctt
     8641 cgattcaatg aattcaataa caagtacata tgctcgaacc ctaagtctaa tgtctgaccg
     8701 atttttagaa tcagcaaggt aatgattact ggacgaattg ccggcaatgt aacatgccat
     8761 gttttgcgta agcgtcctgc tccatccatt tctgcagctt catacaactg tgtatcgact
     8821 gcagttatcg ctgaaaggta gatgatcgta gaccagccca actctttcca gatcacttgt
     8881 ccgatatata ctgtgcgtgt ccattcaggg gatgttagaa aactgatttt ttcaaatccc
     8941 attgttgcaa ggaattcatt caatacccct ccgtcaacat tcaaaaatac ataagtaatc
     9001 gaaacaatga ttacccaaga catgaagtga gggatgtaaa tgatcgtttg aatcccgttt
     9061 ttcaatcgtt tgttcgttac ttcatttaat agtaaagaca aaatgattgg catcggaaaa
     9121 aacacgaaga tatttaatcc gaacaagatc aacgtatttc ttaataatgt aaagaatgtc
     9181 ggttctgtaa acaaacgaat gaaatgtttg aaaccgaccc aaggacttcc cattattcct
     9241 aaaaatggtt gataatcttg aaaagcaata acgagtcccc ccataggaag atacctaaaa
     9301 atcaagaaat aaaggactcc cggcagaatc atcacatata aaaatttatt cgttttgatt
     9361 cctgcaatta ttttttttcg tcttctgagc ttttcttctt ttttcgatag cgtcagttga
     9421 aatggttctt gtttctttac actttccata agcccctcct tagttttgtg aatgtttgct
     9481 gcttatgact tcatgataga taactatctg aattacgtat ataatcattt ttaccgtaag
     9541 taaaaacgct tacatagcaa tgatttttgt gctatacaaa acgataaagt gtattttttc
     9601 acaaataaac tacgcatagc aaaccttcta tttttttcta tctgacactt agcataggaa
     9661 atgattatat acgtaatctt tcaccgatga taaattgaac atatagaaac agaaacattt
     9721 aatttatttt gaggaggagc ctatctatat gaaaaagaaa gtctctatag gagaaagaag
     9781 ttttacactt ttcaacaata cgtttctaat tttgctagct ttgatctgta tcgttccttt
     9841 tttaaatatc atcgctacat cttttgcttc tacacaagaa gtcgtcgcga aaaagtttat
     9901 cctttttcct actacctttt ctttagacgc gtatcgctac attctttcta cgccgaccat
     9961 ttttagagca cttgctgttt cgattggtgt tacaggtgtc ggaacgatcg tcagtatgtg
    10021 tgcaacttca ctgatggcct acggtctttc cagaaagtat ttgtttggtc gaggtttcgt
    10081 caactttctt gttgtatttt ctatgttgtt cagcgggggg atgattccaa cgttcttagt
    10141 tgttcgttct ctagggctgg tcaattctta ctgggccatg atcctgcctg tggctgttaa
    10201 tgcaatgaat atgatcatta tgagaaactt tttccaagcc ttgccggata gtttggaaga
    10261 atcagcaaaa atggatggtt gcacagattt tggtgtattt ttcaaaatta tgctgccatt
    10321 agcgttaccg tctattgcta ctatctcgct tttttatgct gttacttact ggaacactta
    10381 tatgactgct attttgtata tcaacgattc atctaaatgg ccgatacaga ttcttttacg
    10441 ccaaatcgtc attgtatcaa gtgggatgca agcagaaagt agtgccgttg atgtgattcc
    10501 accagcacaa acagttaaaa tggctgttat cgttattgca acagtgccta tgctgattgt
    10561 ttatcctttt gttcagaaat attttgtcaa aggagcactc gtaggttctg tcaaagggtg
    10621 attatgtact gaacgcaaaa tggtagttgt gtcagaagtg gtcagcttca agaaataaga
    10681 aggcatttac gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tttcgaagaa
    10741 tctactcctg gaacaccgtg tatcagaggt tgtgaaagag acgttcagcc ttgagcgatg
    10801 ataaaaactc tcacaaacat tgcgacgagt aacccacagg agcaataaga agcgattttg
    10861 aaaaatagct tcccatattt cacaaaatta tgccttattg tcgatagcct gtctcttgaa
    10921 cgccatttat tcgtaaaaaa gaactgtgac aagacttttg tcacagttct ttttgcgtgc
    10981 ttagccttga aatttaacta tccataaata caaatactag ggttattcta acttctttta
    11041 ttgcttgaat cagtccacaa gaaaaacagc ctcttgattc acaaatattt ttttcgatat
    11101 tctcccggcg taatcgattc cttcttttta aagaaacgaa taaaattttg cggatttcga
    11161 tattgcaatc tttctgcaat ctcttttacg gagaggttag tctctttcaa ccattttttt
    11221 gctacctcta aacgatgatt ttgaacaaaa tcaccaaatt tttctcctgt ttccttttta
    11281 aatacattac ttaaatagtt agggttataa tgcaacctat cagcgatcac atctaaagac
    11341 agatcacgat catattctgt gtagacgata tgcataatat tctccgacaa atttttaaat
    11401 tcatgatcag tgattgcttg agagcattct gtgattggca gaactaattg ttctaaaaca
    11461 agtttttcta gttctttggg atgataattg ttcagtgctt ttacataaat ttgtttcatg
    11521 ttatcaaagt tttttgaatc tactcccaat aactgtccta attgaacgag ttcattgatc
    11581 aagcggaccg aaactacttc ccgactcaaa ggatttttat tttgtgcaaa caatgcatca
    11641 atcaattcat gtactaaatt cttcacttcc tgatttccag aacgaatggc ttcaaacaat
    11701 cgattttgtt cttctatcgg atatctaata agtgtttttt catgagcagc aggaacgatt
    11761 tcctgataaa aaataattga acttggacca gtatttacac gataatacag ggcttctttt
    11821 gcccgatcaa ccgattgctt gctttcattc agcgtttcga accggttact gatgcctata
    11881 ctgatagtta aattcaagta ttcttttaca cgtttttgaa tgagtcggca atactccatg
    11941 acttttttgc ttgcttctgc atcatcttta tccattcgat aaatagtggc ttgcatttct
    12001 tcattcaaaa cgatcggcag cattcgattt tcctttggca caatttccgt gatcatgttg
    12061 ttgatcgcta aaagtagcaa atcacgctcc ccaccgtgac tatcttctaa cagatcgatt
    12121 tgaaccaaag ctgtataata gcagtcattt tgagaactat aaccaaattg ctgcaaccgc
    12181 tgatctagtt ctcgcctttc tactcgattt ctaaataaac taagtacaaa tagcgtttcc
    12241 agctgtggct tttgggtaac taagtgtgca cttaacgctt ctttttctcc tatgatccga
    12301 ctgactgatt gagcgaccaa atctagttct ttcccactga atccttttga tttaagattc
    12361 agacgttcct gtatttgaga aatcggacgg gcaaatctgt ccgaaaataa gtaggaaaga
    12421 agaccaatga gtcctaccag tgttaaagac actgcaacta gcccgattct cagattgcgg
    12481 atcattgcgc caattgcact agagtcaact tctaatctgt aaatccaatt attataatct
    12541 gatttcagta ctatatacgt tttatctttt tctcttatca ctttcagctg attgtaatct
    12601 agatccacgt ccgttaatac agttggttgc tctttatttt ttactgtctg agtagtaaaa
    12661 aggttgccaa cttcgttttc aatagaaagt atgtcttctc cactttcacc aacaacttgt
    12721 tctattgtat gtttctctat cgtagcaatt cccaatgcat atttttcttt ttcaaacatt
    12781 ggcaaagaaa tcaccatttc gattcctttg tttttctttt gccaaaataa attatttttg
    12841 ttatttatgt acttctgttg atacttttca acttcctctt cggtcagttg actcaacgat
    12901 ccattgatta ctccccattt accagccaaa ctgactaaat cataattatt atcatccata
    12961 acaatcgttt ctataaaatt tagttctttc ctgatttcag aataagtgga gtaatctgta
    13021 tatgtcagcg gatgcttgaa tatgtccgag aaatttttgg tcgaaccata cgtagaaaac
    13081 gaatattcaa tcgttctaat tttccattca acgttagttt taacttgcct gagcaaattc
    13141 tttttgtctg ttaaataact cgattcgatc gattcatagg tattctggta aatgaataaa
    13201 ctaaatccta tgacaagtac ggctcctaat aaaaacatcg gtaaaaatat tttataaaat
    13261 actgtacttt tcttcatttt cttcctctcc tccttttaaa ataaaaggct ttcatttttc
    13321 tgtaaaattg atactcatct aattaaatga gcattccccg ctttcaagaa tagcataatt
    13381 aaaaataaaa atccaagtaa tttttaacgt gtgcaatctt ttcatatcaa agatctattg
    13441 acttatcgta gttatcgtaa tatgatgaat gcgttaacaa aaagtgaaaa agtaaggaaa
    13501 atgatggaat ggtaacgatc aaagatatcg ctaaagcagc tggcgtttct catacgacag
    13561 tttctcgtgc tttgaataac agcagtttaa taaaaaatga aacaaaacaa aaaatacgac
    13621 aactagctaa ggaaatgaat tattcgccaa actatagtgc aaaaggttta gtgaatcaaa
    13681 aaaaatatct aatcggacta tttttctcaa gtttgcataa aggaacttca tcaaatttcc
    13741 tagcagattc cattgcaggc atccattcag tattagacac aaattacagt ttatctgttg
    13801 aaagtatcga tgttgttgat ttaacagaaa tcaacctgca acgctacgat ggactgatcg
    13861 ttatgagtca aagtgatgat gatcagcctt tcatcgacta tctcaaagaa aaagaatttc
    13921 ctttcgttgt tgtcaatcgt cacatcaatg atccagcggt tatcaacgtc actgccgatg
    13981 atgcggccgg tgttatgcag gcagtagatt acggtattgg atgcggacat acgaagatcg
    14041 cttatattgg cggaaagaaa aattttcgtt cgacaaaaga acgggaaaaa ggggtattga
    14101 aaagtctaaa aaaagcgagc atccctgtca atccagccta ttttttaaca ggagattaca
    14161 gcttagaaag cggcctccaa aacatggaat acctattatc actttctgac ctgcctactc
    14221 tggttttttg cggcaacgac gacatagcga ttggtgcatt gagagctgta catttagctg
    14281 gcctccgtgt tccccagaac atttctctga tcgggttcga tgattctcca gtcgtctcct
    14341 atctcaatcc gccgcttacc accgtacgta agcctttgaa agaaatgtgt caagaaggaa
    14401 caaagctttt actagatttg atcgaaggca ggacgatcga acaatctaaa attgagctct
    14461 ccaccttgtt gatccttcgt gagtcgatca attacttgta aactacaagt aattttatat
    14521 aaaaatgtta acgtgtgcat aacacagaaa ggaagtttat atgaaaaaac tgacaaaaga
    14581 attagtgaaa aaaaacgaag cacctgtaaa agttcttcag tttggtgaag gcaattttat
    14641 gcgtggattt attgattggc agattcagca gctgaataac caacaattgt ttaaaggtaa
    14701 tgttgcaatc attcaacctt tagaacaagg tcttggaaat atgatgaacc agcaagataa
    14761 tttatatacg gtcattttac aaggactcag caatggcgaa atcgttgata cttccgagat
    14821 tatcaccgta gtagaacaaa tgatcaatcc ttatgatgaa tgggatgctt atctatctct
    14881 agcagaaaat gacgacttgg cctatatcgt ctccaataca acagaggcag gaatccaatt
    14941 cgtggagact gataatttaa cagatactcc cccacagagc ttcccgggca aactgacagc
    15001 ctttttatac cgacgtttca aattaaacaa agctggcttt accatcattc cttgcgaact
    15061 gattgacaga aacggcgaac gtctaaaaga aatcatttta cgctacgcag aaaaatggga
    15121 tttagaacct gcttttgttg agtggttaga aaaagaaaat gttttctgtt gtagtttagt
    15181 tgatcgcatc gtcccaggtt atcctcgcga agatgcaggt gttttcaatc aacggcataa
    15241 ctatgaagat caattgatgg tcaaagcaga accattcatg ctttgggtga tcgaagggcc
    15301 taaagaattg gagaatcaac taccattcaa acaggctggc ttgaatgtca tcatcacaga
    15361 tgatatgact ccttatcgtg aacgtaaggt gcatttgctt aatggaccgc atacagcgat
    15421 ggtccctctt gctttgttgg cggaattgga aactgtagaa gaagtaatga aagatccttt
    15481 attctccgca tatgttgatc agttattcaa tctagaattg attcctatgc tttctttacc
    15541 taaagatgaa ttagccattt atgctgacca aatcaaagaa cgtttcttga atccttttgc
    15601 ccatcacaaa ttagaagcga tttcattaaa tagtgtttca aagttcagca cccgtctgct
    15661 tccagtgttc aagaagtata tcgaagaaca aaatcaggta ccaccgttaa tcactgtttc
    15721 actagctgcg ctacttctta tgtatcgagg cgatcaagta aagccacatg atgatgaaaa
    15781 gacgataagc gaatttactg atgcttggtc tgataatgga acagctattc ctcgattatt
    15841 gcaaaacgct gcgctttggg gagaagattt gtctcaaata ccgaatgtga cagatacagt
    15901 tcaagaaata gctgcaaaga tcgatcaaaa aggtgtccgg tcagtattac aagaaatgaa
    15961 aggaacgatg gtccatgctt gaaaccctgc aaccaacaat gaaattaaat cgacgagaca
    16021 gtgtggtcgt cgcattaacc cctattccaa aacaaacaca actgacgatt gatcagcaaa
    16081 cgatcaccac tcttgaagat atcccgcaag gtcataagat tgcattgggt gatttgaaag
    16141 ctggagacaa cgtgatcaaa tatggctatc caatcggtca tgtgacgaca gacgtaactg
    16201 ccggtcaatg gctgcacact cataatgtaa aaaccaatct ttctggtgaa ttggattatc
    16261 gatatgaaaa agatgtccat ccaagtcatt acctttttga gaaccgaaca tttcaaggtt
    16321 acttgcgaaa aaatggaaaa gtagggattc gtaatgattt gtttttagta cctaccgttg
    16381 ggtgtgtgaa tggcattgct gaactgatcg tcaaacagtt caaggaaaaa catccagatc
    16441 tgggacagtt tgaccatatt acgattttaa agcatcctta tggctgttcg cagcttggaa
    16501 aagatcatca aaatactcga gaaattttag cggacgcagt aaatcatccc aatgccggag
    16561 gagttttagt atttggatta ggttgtgaga acaatacagt tcctgaattc aaaaaaattt
    16621 taggtgaata tgatgaagaa cgagtgaaat ttctagtggc tcaagatgtt tacgatgaga
    16681 tcgaacaagg tgtggctcta ttagaagagc ttttaacagc ggcggaaaat gaccagcgca
    16741 tatcagtccc gctctctaag ttgaatgtcg gtttgaaatg cggcggttct gatggtctat
    16801 cgggaataac tgctaatcct ttacttggtg cattttctga ctacttgatt gctcaaggag
    16861 gcagcacgat cttaacagaa gtgccagaaa tgtttggcgc agaacaagta ttgatggcac
    16921 gagcggaaaa tgaagaagtt tttgactcaa tcgttgactt gatcaatgac ttcaaacagt
    16981 atttcttgtc ttacggggaa ccagtttacg aaaacccatc tcctggtaat aaagcaggtg
    17041 ggatcactac attagaagat aagtctttag gctgcacgca aaaatcagga agctctcctg
    17101 tcgttgatgt gctacaatat ggcgaaaaaa tcagaaaaac agggcttagt ttattgcaag
    17161 ctcccgggaa tgatcttgta gctgcctctg ctcttgcttc atccgattgc cagttggtct
    17221 tgtttaccac aggaagaggg actccgtttg gctcatatgt cccaacggtc aaggtatcta
    17281 cgaatacaac tctttttgaa cgcaaaggtc attggatgga ttttaatgca ggcgtcttat
    17341 tagaacaacc aatggaagta atcttagaat cgtttgttca aaaaatcatc gctgtagcta
    17401 gtggagaaga aacgaaaaat gaacaaaatg acgttcgaga aattgccatt tttaaaaacg
    17461 gagtaacact ttgattttca taaaacagta taaaaagaaa gaaggaagat ttatgttttt
    17521 atctgatgat tttttattaa aagacgattg ggcgaaaaag ctttaccatt cctacgcaaa
    17581 aaacatgccc attattgatt atcactgtca cttagatcca aaagaaattt acgaaaacaa
    17641 caatttcagc aatctgacag aagcttggtt aggaggcgat cattacaaat ggcgcttgat
    17701 gcgagcttgc ggcgtgcctg aagaaaaaat cacaggaaat gcttctgatt ttgaaaaatt
    17761 tttagcttgg tgtcaaaccg taccaaaaat tatcggaaat ccgctttaca gttggtcaca
    17821 tcttgaattg aagcgtttct tcggtattga cttgttgata aatgaagaaa atgcagagaa
    17881 aatttggaca caagcaaatg aaattttggc agctcctgat tttcgccgtc gggaaatcgc
    17941 aaaaaaatcg aatgtagaag tgatttgtac aacagatgat cccgcagacg acttgcacta
    18001 tcatcagttg cttgctcatg aagaaccaga tttgaaggtt ctaccttcat tccgtccaga
    18061 taaagcactc aatattgaaa aagaaggatt taatgcatgg gtcacactac tggaagaagc
    18121 agctgaccaa acgatcacca cctatcaaga actgatagaa gtcttagggc agcggataga
    18181 atacttccat caacatggat gtcgtgtttc cgaccacgct ttagacatct tacgctataa
    18241 agaagcggat gaaacagtat tagaagagat ttttcaaaaa gcacgagcta acaaactatt
    18301 atcttcagat gaaatcgatg cttatcgtac agaaacactg atacatttga cccacttcta
    18361 tcattctcat aattggacga tgcagctgca catccatgct tatcgtaact gtaatacaca
    18421 aatgttggaa aaattagggc ccgataccgg ctatgatgga atgaatgatc tttctttgac
    18481 tattcctctg caaaaattat tgaaccgtgc agaagaaacg gatcagttgc caaagacgat
    18541 cctttattca ttgaatccga atgactatcc tgcgatttta gctttgatgg gctgtttcca
    18601 aaaagaacag aatggaaaac tgcagttagg ttctggatgg tggtacaacg atactcgagc
    18661 tggtatgcgc gatcaattga cacaatttgc tgatggtagt gctttgggga attttgtcgg
    18721 catgctgaca gattcaagaa gtttcctgtc ctacactaga catgaatatt tccgtcgtgt
    18781 tctttgtgaa tttttaggcg aaatggtcat gcgaggagaa gcacctgaag atgaacaact
    18841 tttaggaagt ctagttcaag cgattagtta tacaaatgct aaaaattact ttggcttttt
    18901 tgcctaattg attaatagta acagtaacga atcaaaaaca ggatagtgaa ataacggaat
    18961 ccgtttatcc acgttttaga gaagaagacg gaccaaattt gctccgtctt cttctcttgc
    19021 tgttctgctt cattgcctat aaacctttat gaaagaagag ataacatgcc tactatccat
    19081 attgccggtg attctactgc ggcagaaaaa gatgaaaaca agcgtcccga gacaggttgg
    19141 ggagaaaaaa tctctgctta tttttcaccc gagatccaga tcaataacca agcaaagaat
    19201 ggacgcagca ccaaatcatt tatccaggaa ggaaggttag cccgtctagc ttcacagttt
    19261 cagccgaatg atttcttatt tgtacaattt ggacataacg accagaaaat ccaagaagaa
    19321 agaggtacgc ttcccttcgg tagctattta aaaaatctgc agatattttt tgaatgcgca
    19381 aaaaaagcga acgtccaaat ggtcttactg acatctgtga cgcgacgaga ttacttagaa
    19441 aacggaacat tgaatccaga tatacttgga gactatccaa aagcgatgcg tgcatttgct
    19501 gagaaacacc atattcctct tttagatgtg ttctcaagat cacaagaatt gttccagacc
    19561 ttctcaaaag aagagacaaa aaaattctac ctgcatctta tacctgatac ttgtaaaaat
    19621 tacccagaag gactaaaaga caatacccac tttagtccag aaggcgctga taaagttgcc
    19681 cgaataattg ttgaattgat aaaagaatcc cgtctgcctc ttgctaatta tttacaaaaa
    19741 ggagtttgag atgtttccat taaatttagg catccgtgcc cacgatttag atgttcgaaa
    19801 tcgtgaagaa atcgttcaaa aagtatcaca gcttcacttg tcccatatcc aatttgcacc
    19861 acaaaaagca tttcctgaac aacttaaatt aactgacatg acacttggga gtgctgccta
    19921 ttttggtgaa tacttccaaa aaaacaatat cgaactttcc attttaggat gctatatcaa
    19981 cctatccagc aaaaatcaag aagttcgtgc acaagcattg gcaactttca aacagcaaat
    20041 tgctctgtgc tctgcctatc aagccaagat gattgctacg gaaaccggta gtgtctctgt
    20101 cggttacaca aaagaaaatt ttactgaaga agcttatcag atcgccagaa attctatcat
    20161 tgaattagtc gctcatgcag aaaattttgg cgtaactgtt gctgttgaag cagggatcaa
    20221 tcatccaatc tataactatc agttagctaa acgtttaatc gatgaagtag cttcaccaaa
    20281 tttaaaaatc attttagatt gtgcgaattt gatgcataaa gaaaatcatg ctgatcagga
    20341 aaaagtgatc cgaggtgcct tggacaattt acatggttat attacagcgc ttcatctcaa
    20401 agactatatc atggaaaatg gaaagatccg catggtacct gttggcaagg gctgtttaga
    20461 tttccgcccg gtactgcatt atatcaaata tgagcgtcct ctaatgtacg ccactcttga
    20521 agctactcct gagatttatg ttcctgaagc aatagaacac ttgcagttta tttaccaaga
    20581 ttgttaagga gaagattatg gaggaacgaa tacagaaaag ttatcaaaaa tatcatgcaa
    20641 atgcttttca gacattgctg catctttatc agggaaatta ttggcgtttt gccatgtcag
    20701 gactcttttt tattatcaaa catcttccat cttggttaat gccgatcgct attgccaatg
    20761 taatcaatgc agcttcatcg aaagattctt ctcagttgca ggccatttat ttcaacgctt
    20821 ttttgatggc ttgtctgatt ggtcaaaata tattgacgaa ttattttcat gtaaaatacc
    20881 atagcacttc gatccgtcaa gtagaagctg gactaagagc gacccttatc aagaaaatcc
    20941 aagaactatc tattccttat caaaaagaat tgcaatctgg acgaatccag tcaaagatca
    21001 tccgtgatgt agaagctatc caaacgttgt cctctcaagt atttgtcagc ggattaaata
    21061 tcgctgtcaa tttattcgta gctttaggta tcacgcttta taaaagtcct gttgttttca
    21121 tgtttttctt attgactgtt cctctcgccg cattcgttac tctctttttc cgtaaaagga
    21181 tcagacagtc caactcagac ttcaggcaag aaatggaaga gacttctgca aaagtgatcg
    21241 aagtgatcga attagcaccg attgctcgag ctcatgggtt agaaaaacac gaaacaactc
    21301 gcttaaataa gcattttact caaatctatg aaaaaggatt ccacttagat atcctgcaat
    21361 ctgttttcgg atcagtcagc tgggctagtt ttcagatatt tcagctgatt tgtttgattt
    21421 tcactgggat attagcttta caagggcgca tccaacccgg cgatgtcgtg atgtatcaga
    21481 cgtatttcac gacgatcgtc aattccgttt ctggaatgat cacgctgatt cctacgattt
    21541 caaaaggcat ggaatctatc acttctattg gcgaactgtt gattgctggc gatgtagaaa
    21601 cttatcacgg taagaaaaaa atccaacatt tggatggaaa ttttcgtttt gaggatgttt
    21661 cttttcttta tccggactca ctcaaaccga ttatcgatca tttttctcta gaggtgaaag
    21721 ctggagaaac aattgctttt gttgggccat ctggctctgg aaaatctaca ctccttaact
    21781 tattgattgg atttatccag ccgacttcag gaaaaatttt tctagatgat cagcctttgg
    21841 atgaactgga tatgcgaagt gtgcgaaatt atttagccgt tgttcctcaa accactttgt
    21901 tgttctccgc ttcgatcaaa gagaacatca cttatggctt gaaaaacgta tcaaaagaac
    21961 gtttagatga agtcatcgaa gctgcgcaac tttcttcact gattgaccag cttcctgatg
    22021 gtctggatac cttagtaggc gaacatggca acaagctgtc tggtggccaa aagcaacgta
    22081 tctccattgc acgagcgttg atacgtcagc caaaaatcat tttgctagat gaagcaacct
    22141 cagctttaga taaccagtca gagaaaaaaa tccagcaagc cctgaattac ctgacagaaa
    22201 cacccactac ttttattgtg gctcatcggc tttcaacgat caaagaggca gataaaatcg
    22261 ttgtgatcga tgaaggaaaa atcgttgaaa taggtactta cgaaaaactg ttagctcaaa
    22321 aaggctattt ctaccgcttg aaaaatgaat gacaaaaaat aggagctatg acaagacttt
    22381 tgtcgtagct cctatttctg aataaacggt gttctcctgc caactcctcg gtcacaagtc
    22441 acaatagtca tcaaacatga gaagctgttt tttgaaattg ttccttgtaa ctcagagtta
    22501 aacggtaggc tcacgacctc ttggtaaatg gtgttcaaga gacagcctat cggcaataag
    22561 aagcaatttt gtcatcgctc aaggccgaac gtctctttca caccctctgt tgtttcataa
    22621 aatagtttta ttcgctgtaa aggtcgcgaa ctggtgtcat gatgatacgg ttcaagtcat
    22681 tgatgattgt actgaagcct tgctcttttt gcatcaagtc attgattact tcttctgatt
    22741 ggattttcac agccatttct tgtgctttca ttgcatcttc gtcgctgaac tcttgtccac
    22801 tcatttgttt ttcttgtaat tcgatttgca tcgcttggaa gtctttgaac agtttgtgtg
    22861 cttcttcgtt tgcttgtact ttctcgtacg cttctttcaa cgctttgaat tctggtaatt
    22921 cacggatctc acgttcgatt tgattcgcgc tatcgtagat gtttgacatg ttatgccctc
    22981 ttttctttta gtagttactc tctattgtaa catggggtta ttcgttttca aaggggctac
    23041 tctccttgat atttccgcca gaggtcttta gccgcttcac caattccctg aacgccttct
    23101 ttcaccatat cgccgaagtt tttcagccct tcttttgctt gttcaccata ttgattcaag
    23161 ttatcttgcc aatttccaga ctctccatta gaatttcctg tgtttccaac ttgatctgca
    23221 gctaccactt gtcctcctgt ggcataggca tctgctacac taaatcctgt ttttgaagta
    23281 tacggaagga tgccttctgc cgctgatttg aagactttcc cgacaacatt tccacttgtc
    23341 ccttccaaat agtgcagctt gctggattct tcgtaaccca tccatgtcga aatcactacg
    23401 tctggcgtgt aaccgatcat ccattggtca tttgcttttg tggcatcgaa attagtttct
    23461 gtggttcctg ttttccctgc tatcgtataa cctgcaggat tcgcagcagc cgctgttcca
    23521 tttgaaaatg taccaagaag catgctggtc atttcatcgg ctgtttcctt tgatatgacc
    23581 tgttttggtt ttttgtttgt gttatcgacg atcaccgctc cagtcgaatc aacgattttc
    23641 gtgataagat gcggcgtata catctgacca tcattggcaa atgcactgta agcacccgcc
    23701 atgatcaatg gagatactcc tttttccagt ccaccaaggg ctaagccata atacttatca
    23761 gattttgcta aaggcaggcc gaattcttct gctttatcat atcctttttg taaaccgatc
    23821 tcatgcaaaa gccatacagt aggcaaattc aaactttgtg caacagcttg atacatcggt
    23881 acttctcctt ggtatgtccc gtcaaaatta tgcgcatcgt aataactttg cggttcgtct
    23941 ttcaagatac tggaaggctt gtatccagct tccagtgctg gtgtatatac gcttaatggt
    24001 ttgatcgttg atcctggtga acgacgcatc tgtgtagcaa agctgaatcc tctaaagaca
    24061 tgatctcctc ttcgtcctac tagtgcttgt acaccacctg tattcggatc taaagctact
    24121 gaagcagatt ggaccatggc accatctgaa gcatttgctg ggaataatgc actgttttga
    24181 taagtagctt ctaattgctt ttgataatcc tgatctaaag ctgtatagat tttatagcct
    24241 ttattcatga catcttcttc atcaagacca tactcgttaa ctgcttcatc gatcactgca
    24301 tcaaaataat aaggataacg ataaccagaa tctgaattat cgtatgtgtc attaagatac
    24361 gcgctgatat ctgtctggct ttctgcttgt tcttgttctt ttgtgatttt tccgttttct
    24421 tccattacac ttagtactgt gtttttccga tcattcgcat tttttgggtg atcgatcgga
    24481 ttataaatac caggaccttt cagcattcct gccaaagtag cagcctcaga cagcgtaaca
    24541 ttcgttgcat ctactccaaa atactttctg gaagcgtctt ggacacccca taccccgttg
    24601 ccaaaatagg cattgttaag atacatggtc aagatttctt ctttgctgta cttcttttcg
    24661 atctcgattg ccaaaaataa ttccttcgct tttcgatcaa acgtttggtc taatgtcaga
    24721 tacgcatttt tggcaagctg ctgagtgatc gtacttcctc cgccccctga tgttccgcca
    24781 gacaagacca ttcggactgc tgcacgggca atccctttga tatcaaaccc gtgatgctcg
    24841 taaaaattcc tatcttctgt cgagattact gcatcttgga tatagggaga aatcgcgttt
    24901 aattctacat atgttccttt ttgggcgtac agtgtgccag cttcttcgtt tttcttatca
    24961 taaatgactg tcgattggct taatcctgat tttaacgttt cgacatttgc ttgttttgct
    25021 aaaataaaga ggtagatact tgtcactaag acacagatca aaccaatcag caaaatcaat
    25081 ttgttcacat gatatttttt ccagatgcgc tttcgccatc gatggaattg tccgagatat
    25141 ggtctgatcc aacgccaaaa actgatcaaa gcagctttta tcttttctaa aatgctttga
    25201 aaatccattt gcttcttctc ctgtctattt gattcaatcg tcttatttta ccatagtgaa
    25261 agagtgtttt cttcctgctt aagttgtagt tcaatatgtt tttttacgtt agtgcaagta
    25321 gagtaacata agaatgaaca gaaaaatgta agttttttct taaatatgtt ttaaaaatgg
    25381 tacaattagt ctgcgattgc actgaaagga gtacacttgt gaaaatcacg atcactttac
    25441 ctatgaacca tcctgaaaca acaattcgcg acttgcttga aaaggaatgg ttagttccta
    25501 gaaaggttcg acactttttg cgtatacgta aaaacgtttg gatcaatcaa caacctgctt
    25561 tgttccatca tgaagtaaag ggcaatgacc agatcacatt gttattcgaa gaaacagatt
    25621 ataattatca agaagttcaa ttaggagatg cgaagcacat ccgtgtttta tatgaagatg
    25681 agcatttgat tatagtggat aagcctgcag gaatgaagac tcatccaaat gaaccgacag
    25741 aaaacagtac cttactcaat catttagctg cttatctcga cactaaacag caacgacctt
    25801 acgttgtcca ccgcttggac aaagaaacaa gcggtgccgt tttatttgca aaaaatccat
    25861 ttgtcttgcc gatacttgga agaatgttgg aacaaaaaat gatctatcgg agatatcaag
    25921 ccatcgtatg gggagaaatt gccaaagata tgaccatcac caataaaatt ggacgagatc
    25981 gccatgatcg gagaaaaagg gtcgtagatg ataaaaatgg tcagagcgcc ttgacacatg
    26041 tcactgtcaa caaagtactt tctgaacaaa cacaagtcta ttgtgttttg gaaaccggac
    26101 gtacacacca gatcagagtt catttagctc ataagggaca tccgatcgtt ggagaccctc
    26161 tctaccaaaa tagaacagct gaacgactga tgctccatgc attagaactg cacatgatcc
    26221 atccatttac tcaagaaaaa atagtcggta aagctttacc tggattatgg gaatagaaat
    26281 aaaaaagaac agagtcttat cagcagataa tgataagact ctgttctttt tgtgtaattt
    26341 gtctgcttac ttttgatgtt cgtgttctgc taacagcaaa ctaccgataa tcccactttc
    26401 attcggcagt ccccaacgaa cgatatattc acttacttga ggtgtttcca tataaccacc
    26461 catcaattca acaaattgct gacggatttt ttgaaggaga tgatcctgat tcattacccc
    26521 accgcctaaa ataatctttt caggtgctaa cgtcaacgta tagttgatca gtgcttgagc
    26581 aatataataa gcctgaatag cccaaacagg atgatcttgc ggcagatctt gccctttgat
    26641 ccctgtgcgt gcctcaatag acggtcctgc tgctaatcct tctaaacaat ctttatgata
    26701 aggacaagta ccttcataat ggtcatctgg atggcgtttc acccaaatat gtcccatttc
    26761 aggatgtgcg atccctgaaa aaatttcttc attgatcacg acacctgcac cgattcctgt
    26821 tcccacggta aggtaaatac aactattttt tccttgtgct gctccttgct tcaattcccc
    26881 atatgcagca gcattgacat ccgttgtcca agcagcagga atattgtagc gttccttgat
    26941 tgtgccaaga aaatcgaatt gtgcccaccc ttttttggga gttgccaaaa cgtatccgta
    27001 attatctttt gttttgtcga taccaatcgg gccgaatgag ccaatcccga ttcccttcaa
    27061 ttcataacga tcaaaaaatt caaatacctg atccaatgtt tcttctggtg ttgtggtagg
    27121 gatacttaat ttttctaaaa tttctccacc atctgaaacc gcacacacaa attttgttcc
    27181 gcctgcttct attccgccat acattcgttt tcctcctcga atccgattgt ccgaatctca
    27241 taaggttgga ttattgggac aaccgcttgc ttcgttggtt cttcaagcag attcaatatg
    27301 acagtttttc cattgtcttt agttatttcc aatttttcta aatgactaga catattgaaa
    27361 cctcttacta ctactttatc actaaacttg ctgcgtttca aagctgtaat tgcaaacgtt
    27421 tcacttttta tcgttaaata ttgttgtttt gaaatcaatg ttccttcatg atgtttgatt
    27481 tgttgagtgg aaaaaggaat ctgtgctgcg tatgcatgca aataagtaga gaatttttct
    27541 tctggtccat gtagttcaat gctgtaattg aaccgatgct cacctagaca ctgtgcttct
    27601 ggtgttggaa aatatcccca atcgcctagt tctcccacac tgcgaagtag cgtcactgcg
    27661 atttgtccgt cttctgttac ttcatattca ttcaaaccaa agtttccgac tgttacaccg
    27721 tactcttcat catgaatatt gacaaatgct tgctgatgtt gcggattcgt ggggttttcc
    27781 caacttttag acacatggtt tgggcgcttc accacttcaa aaatactatc tgcttcgtga
    27841 tgttccacat gtaactttgt aggaaacaag acacgtaagc gatgatcttt catctgattg
    27901 tcgagagtcg tttcaaagtc gattttttta ctatcttttc tgattgtcat gattgttttt
    27961 atttgaagga ttcgtttttc tttggatcgt tctgcttttc gataacgaaa ttccacaacc
    28021 atctgctgtt ctttttctaa acgctcgtct gcagaaacag ggatttccat ttcctgtaga
    28081 atacttattt ttgctatttc cggtgtatct actaatactt ttacttcaga gttttctttg
    28141 ttactggata ggattggctg atcacagaaa ggttgtttaa agatatactc atttcctata
    28201 tcgccgatat cttcaaaaat atgcagatta ttcaactttc gtttgttttt gcgatcttcc
    28261 attgtaatgg taccattttt ttcaatcgtc aaatgtagag ggccgttttc gatgattcgt
    28321 ccactctgat gaatcattgt ttccttttct tctgctttcg cttccccttc tacaagcgcg
    28381 aatgtttccc aagaaaatgc tggcatgttt tctaatggta aagtcacttt gatcattctt
    28441 gccatatatg gcacacggaa acggtctttc ggcaaatcat aaccaaattg gacagtttct
    28501 tcagataaaa tagctggtat agtggcccca ctttttgttt ctacatgata cttacgttta
    28561 ggcaaatttt ctaactcttg atatagttgt tcaggtattc cttcagcgaa tctttttctt
    28621 tctaattcga tggttatttc tgcttcacca gttttatcca ttcctgcagt atttacaatg
    28681 acaaatggaa agctgtcttt tgggaatccg gtcgtatcga ttgctgcggt cagctgttct
    28741 aaggaatctt gtgcaagata ctttccaact tcatctgctt tttcaaaacg aggtatcatt
    28801 tcccgatgga cagaatcgac tgaacaaccg caaatactgt cgtgaggatg attttgcatt
    28861 aatgtcttcc aagcataatc taattgatca tggggatagt ttccactaac ttcataagcc
    28921 atcgtcgcaa gtggttctgt aatattttct agctgtcgct ggaccttggt attccattgt
    28981 tttaagtaaa cccgagcaga tgctgtattg gctaaagtat accagccatc cgtttcttga
    29041 cttgtcaatt ctccttctac tgagcctaag tcttctggca catctttttg aactgcttcc
    29101 agatagtcag taaaattaga atgaataaac tcgtaatctg gaaacagttc atttgcgaga
    29161 tggattgctt ttgatatatc tttttgtacc ggttgatgat caacaccatt catcatcaat
    29221 aagtggttcg tagaggcata ttgttctgca tccgctaatt tttgcttcca aaaggcaaga
    29281 gctgcttctt tttctactgg aatctcattc ccgttgctat accaattggc aaatagcaga
    29341 ccaaagattg ctgtttgatc aggtcctttc caccacatct ctgagtattg ggaagaatag
    29401 ttttctgctt ccaaaacttg attatcaaag ccaattggtt ttacaccacg gccaaacgct
    29461 gctgctgaaa taccagcttg tttcattaat tgaggcgtct gtcccatatt tccaaatgta
    29521 tcgggaaaat agcctaacat gacaggggtt ccccatttac ggctttcttc catgccaatc
    29581 agcatatttc tcacgttcga ttccgagctg ataagaaaat catcttgcaa gatataaaat
    29641 ggtccaattc tcaattttcc ttcattgatt gcttgttgga ttgcctgtct tttttctggc
    29701 cttacttgaa gatagtcatc taaaataatc gtttgcccat ccaaatgaaa actgttgaag
    29761 gacggatcaa cttcaaatag ctctagtaga tcgtctatca attcaatcag tcgcatgtga
    29821 tgctgttcgt aagccatgta ccattcacga tcccagtggc tatgagaaat gatatatact
    29881 ttctttttca tttacgatac ctcctacttt tctactctaa tatcaaagta atccatgact
    29941 aattcgcaaa acatcatatt tgcccaagaa aaccattctc tcgtataatt ttcaggattg
    30001 ttcacatcga atccttcgtg cataaggtgt gttcctgcgt cagttgctac aagtaaattc
    30061 aatatccgct ctttttcaga tttgtctgac gttgtcattc cttccatagc taatgcaatc
    30121 ggccaaacgt atttttctgg cgtatgggag gatccaattc cttttgcgta ctttccttca
    30181 tagaaatatg gattttcttt actcaacaat gttcttcttg tttgcagata atcttcctct
    30241 tgttcagaac aatatcccaa gtaaggtgcg gcgatcagat tgggcacatt actgtcatcc
    30301 atgactgtgg cgtttcccat tccatctact tcatatgcat aaatcgtttg ccctttttga
    30361 ttaaccgttc gtccgtgatt ctgaatcccc tcttggatct ctttcttcaa tttctgtgtt
    30421 tgctgtacaa tttctttatc atcgagtatc gtttgaaaaa tttcttccac ataacctaac
    30481 acgaccaccg caaacatatt ggatggtacc agatatccat aacggcaagt atcatcgcta
    30541 ggacggaaac cagaccatgt cattcctgta aaagctgttt ttgcacctcg cccgtcattg
    30601 atcagcgtat cttcttttct tgtcgtgtct cgtacaaatg tataaggaga gtgttcatga
    30661 tcttgctctg ttttgaacac gtccaagatt tttttgatac cttcaataaa agattgattg
    30721 aattgatcag tttttcctgt atttttgtaa agaagatagg ccaactgtat tgggtagcat
    30781 aaagaatcaa tttcatattt tctttcccag atccaatcgt tcatttttgt atgatctgtt
    30841 tggtggccag caccgttagc ttcttcgttg aaggcattcg catatggatc gatattgata
    30901 taaaaaaatt gctttttgac taacccgctg atcatgtcag ctaattcctc atcttctttt
    30961 gcaatcacta aataaggacg aacttgtgca gtagagtctc ttaaccacat cgctggaatg
    31021 tctcccgtca ataaaaaggt ggttccgtca ttttgctttt tgactgttgt caacagagta
    31081 ttggcaaatg ctgctgtaaa attctttgcc cagtccgcat ggatatcccc acatttttgt
    31141 gtgatatctt ccataaattt ctgcaccgat tttggtatat ctttataagc cactaataaa
    31201 accttctttc tatttgatat atctttaaag attatacttc atacagccaa ttatgtctac
    31261 gctttcaaaa aatatctttt tatttacttt ttcatccatt atggtattat ttgaatatga
    31321 tatatcatta agaggtgttt atatgaatca accattgtac cagacaatat ttaatgattt
    31381 gcaaacggct attttaactg gtgaattacc tgtagacagc caactaccaa ctgaaaaaga
    31441 actttcacgt acctataaag taagcagaat cacttccaaa cgtgcattga ctgaattaga
    31501 gcaattagga cttatttatc gcgttcgcgg gaaaggcagt ttcgttaaat ccgcttctcc
    31561 ggaacagatt cttgcttctc ctacaaaaac acgacggatt ctgtttcttc ttccatatgt
    31621 cgctgatttg tctgttggtg attttagcaa gggattgaac cctgtgatgc aagataaaca
    31681 gattgatgtc atgatgacga cgcttgactt tctacaaaat aaaacagcaa aagagctaat
    31741 caatgaattt gacggattga tttattatgc tcttgataca gaacaacatc ttgacttact
    31801 ttttgagctt tcattgaaag aattccctgt cgtcatatta gataaaaaaa tctatgagtt
    31861 accttttcct accgttcttt ccgataattt ccaaggcggt gcgcttgctg cctcttattt
    31921 gatcgaacag ggacattcaa aaatcgctta tttgtttggt acgcagactc atccacaatc
    31981 cgttcgacag cgttaccttg gttaccttga agcattggac aaagctggtt tgactttcca
    32041 cacttcactg gatgacaaaa gcgcgacatt agatgaattg ctttcttata tcactaagta
    32101 tgaagtaact gcttttgttt gtgaaaacga tgttgtcgct atccagacca tgaatctctt
    32161 gagacagcat ggttatcata tccctgaaga tttttcaatt atcggatttg atgatatcca
    32221 agcagcagct cttgttgacc cgccactgac aaccatcgca caagattttg aacagttagg
    32281 taagcttgcg ggagatgctt tggtcacttg gttagagaca aaagatcgac catccgatag
    32341 taaatttcca gtttcattca tcaaaagaca atcaacaaag gagaaataaa atgaatatcg
    32401 gtcaaataga tactagacat gcaacagcga atcagccaaa tttctctaat gggaactgcc
    32461 agccttatac tggcgttcct tttgggatga attattttgc accgcaaact acggatcaaa
    32521 acggcagctg gtggtttcat cctgatgacc gcgtttttca aggctaccgt cttacccatc
    32581 aaccaagtcc ttggatgggt gatttcagtc atatgctgtt tacgccgatc aatggaaaac
    32641 tacaggagaa cacccttttc catgctcaaa gttcttaccg cccagaagaa agtatatttt
    32701 gcccgactca tctgtcgatc tgtcaattac gttatggcat ctgttctaca ttgataccta
    32761 gcatgtatgg cggaatctta tctattgatt acagcaaaaa tgacagcggt ttacttttat
    32821 cctttccggg acgccatcag ctctttgtta tagatcctta cacagtggaa ggaaaaatca
    32881 cacattttgc tggttcagaa gatcctgatt tttcttttta ttttatattg agatttgaat
    32941 atcctttaac aattgatgag ttagctgttt ccaacaaaaa tagcgactcg atcccgttgt
    33001 attttgaaca gacaaaaaaa caaactatcc gatttgggac atcttttatc agctcggaac
    33061 aagcagctta taacctggaa ctagaacaaa accaaagcaa agagacttac cttgctcaca
    33121 gtcaagaaca atggcagtcc tattttgatc gtatacagct cacacaccat gatcaacaac
    33181 aattgcggac gttttatcat aatttatatc gggtgttttt atttccacaa acattttatg
    33241 aaattgatcc tagtgggcaa aaaatccact atgatacgac tagtcgatct ataaaacctg
    33301 gtgttctcta tacaaataat gggttttggg atacgtataa aacggtttat cctctttatt
    33361 ctttgattgc tgtagaaaaa tacgaagaaa tgctggaagg ttttttgaac agctatagag
    33421 aaagtggcta tttgccaaaa tggctgtctc ctgatgaacg tggacttatg cctgggacat
    33481 tgatcgatgc ggtcattgca gatgctgctg taaaagatat ccgacgagac ttgatgcctg
    33541 aatttctaac ggcaatggaa aaaggtgcta cggttcaaag caaacatcca aattatggac
    33601 gccaaggaac agaagattac ttgacatatg gctatgttcc taatcactac cacgaatcag
    33661 tcaatcacac attagactat gcttatagcg atttttgtat tagccaagtt gccaaggtac
    33721 tagaagaaga ggaaatgacg aagaaatatc agcagcaatc attgaactat caaaatatct
    33781 ttgactatga gtcaggattt atgcgttcaa aagataaaga tggcaagttc agagaaccat
    33841 tttcagctaa tcgttgggga caagattatg cagaaggaag tgcttggcaa agcagctttg
    33901 ctgtttatca cgatttttca ggactgatcg gaaaatatgg tggcagtgag agatataaag
    33961 aaaaactcat tgagctgtgc aatcagcccc caacgtttga cccaggagga tatggttttg
    34021 agatccacga aatgagtgag atggccgcga tagaatttgg acaattagct atttcaaacc
    34081 agccaagttt tcactatcct tacttgttca gttatatagg acaacctgaa atggcacagc
    34141 cactgcttaa acagttgatg actcagacct tcgacgattc tccaatgggt taccctggag
    34201 atgaggacaa tggaagtatg gccgcttggt atatcttcaa tagtctggga ttttatcctg
    34261 tcacgcctgg aaccggtgaa tatgttatcg gtatgccatt aattgacaag gctgttttgc
    34321 ttttgtcaaa tggaaaggaa ttgacgattg aagcaacgcc taatcggccg cagcaacaat
    34381 ttatccataa aatcaaacgg atggacaaag aatatacgaa attatatttt acccatcaag
    34441 atttgctgaa aggcggcacg atctcttacc aattagggat catccctcac cctaagaaat
    34501 atcaagcaaa agatttgcct ttttccttat caaactataa ttaacaagag gttgtgaaaa
    34561 agctgtcctg catcaagtaa taagaacagc caagcaaaaa tagatcctca tatttttcgt
    34621 ttggttggtc ttattaccgc agatgcaagc ttttgaacac cgtttattga gaggttgtga
    34681 aatcagcgtt ctgacctgag tattagagag acaaatggca aaatagttcc tcatattttg
    34741 ccattttttg agctaatacc gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt
    34801 gaaaaagctg tttagtttcg agtattaaga aaaattttgt gaaaatg
//
LOCUS       DAJSMI010000008        34776 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJSMI010000008 DAJSMI010000000
VERSION     DAJSMI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.008"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(327..749)
                     /locus_tag="ON430_000358"
     CDS             complement(327..749)
                     /locus_tag="ON430_000358"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR3218308.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(763..2169)
                     /gene="atpD"
                     /locus_tag="ON430_000359"
     CDS             complement(763..2169)
                     /gene="atpD"
                     /locus_tag="ON430_000359"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR3218309.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2192..3094)
                     /locus_tag="ON430_000360"
     CDS             complement(2192..3094)
                     /locus_tag="ON430_000360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR3218310.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3108..4664)
                     /locus_tag="ON430_000361"
     CDS             complement(3108..4664)
                     /locus_tag="ON430_000361"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR3218311.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4687..5229)
                     /locus_tag="ON430_000362"
     CDS             complement(4687..5229)
                     /locus_tag="ON430_000362"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR3218312.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5216..5740)
                     /gene="atpF"
                     /locus_tag="ON430_000363"
     CDS             complement(5216..5740)
                     /gene="atpF"
                     /locus_tag="ON430_000363"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR3218313.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5831..6046)
                     /gene="atpE"
                     /locus_tag="ON430_000364"
     CDS             complement(5831..6046)
                     /gene="atpE"
                     /locus_tag="ON430_000364"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCR3218314.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6099..6818)
                     /gene="atpB"
                     /locus_tag="ON430_000365"
     CDS             complement(6099..6818)
                     /gene="atpB"
                     /locus_tag="ON430_000365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR3218315.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7120..9963)
                     /gene="rnr"
                     /locus_tag="ON430_000366"
                     /pseudo
     CDS             complement(7120..9963)
                     /gene="rnr"
                     /locus_tag="ON430_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(9966..10721)
                     /locus_tag="ON430_000367"
     CDS             complement(9966..10721)
                     /locus_tag="ON430_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3218316.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10824..11060)
                     /gene="secG"
                     /locus_tag="ON430_000368"
     CDS             complement(10824..11060)
                     /gene="secG"
                     /locus_tag="ON430_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR3218317.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11178..11456)
                     /locus_tag="ON430_000369"
     CDS             complement(11178..11456)
                     /locus_tag="ON430_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3218318.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11569..12537)
                     /locus_tag="ON430_000370"
     CDS             complement(11569..12537)
                     /locus_tag="ON430_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3218319.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12752..12796
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12820..13794
                     /locus_tag="ON430_000371"
     CDS             12820..13794
                     /locus_tag="ON430_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCR3218320.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13799..14374
                     /locus_tag="ON430_000372"
     CDS             13799..14374
                     /locus_tag="ON430_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3218321.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14441..15580)
                     /locus_tag="ON430_000373"
     CDS             complement(14441..15580)
                     /locus_tag="ON430_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCR3218322.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(15980..17278)
                     /gene="eno"
                     /locus_tag="ON430_000374"
     CDS             complement(15980..17278)
                     /gene="eno"
                     /locus_tag="ON430_000374"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR3218323.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(17394..18149)
                     /locus_tag="ON430_000375"
     CDS             complement(17394..18149)
                     /locus_tag="ON430_000375"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR3218324.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(18253..19443)
                     /locus_tag="ON430_000376"
     CDS             complement(18253..19443)
                     /locus_tag="ON430_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR3218325.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(19553..20554)
                     /gene="gap"
                     /locus_tag="ON430_000377"
     CDS             complement(19553..20554)
                     /gene="gap"
                     /locus_tag="ON430_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR3218326.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(20595..21686)
                     /locus_tag="ON430_000378"
     CDS             complement(20595..21686)
                     /locus_tag="ON430_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCR3218327.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(21835..23169)
                     /gene="rpoN"
                     /locus_tag="ON430_000379"
     CDS             complement(21835..23169)
                     /gene="rpoN"
                     /locus_tag="ON430_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR3218328.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            23229..23300
                     /locus_tag="ON430_000380"
     tRNA            23229..23300
                     /locus_tag="ON430_000380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23262..23264,aa:Arg,seq:ccg)
     gene            complement(23492..24376)
                     /locus_tag="ON430_000381"
     CDS             complement(23492..24376)
                     /locus_tag="ON430_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR3218329.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(24463..25362)
                     /locus_tag="ON430_000382"
     CDS             complement(24463..25362)
                     /locus_tag="ON430_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCR3218330.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(25379..25885)
                     /locus_tag="ON430_000383"
     CDS             complement(25379..25885)
                     /locus_tag="ON430_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCR3218331.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(26074..26694)
                     /locus_tag="ON430_000384"
     CDS             complement(26074..26694)
                     /locus_tag="ON430_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCR3218332.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(26770..27207)
                     /locus_tag="ON430_000385"
     CDS             complement(26770..27207)
                     /locus_tag="ON430_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCR3218333.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            27370..28200
                     /locus_tag="ON430_000386"
     CDS             27370..28200
                     /locus_tag="ON430_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCR3218334.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            28184..29551
                     /locus_tag="ON430_000387"
     CDS             28184..29551
                     /locus_tag="ON430_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3218335.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            29570..30379
                     /gene="yidA"
                     /locus_tag="ON430_000388"
     CDS             29570..30379
                     /gene="yidA"
                     /locus_tag="ON430_000388"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR3218336.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(30422..30865)
                     /locus_tag="ON430_000389"
     CDS             complement(30422..30865)
                     /locus_tag="ON430_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCR3218337.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            30999..31370
                     /locus_tag="ON430_000390"
     CDS             30999..31370
                     /locus_tag="ON430_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3218338.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(31539..32321)
                     /locus_tag="ON430_000391"
     CDS             complement(31539..32321)
                     /locus_tag="ON430_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCR3218339.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(32318..33289)
                     /locus_tag="ON430_000392"
     CDS             complement(32318..33289)
                     /locus_tag="ON430_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCR3218340.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            33381..34775
                     /locus_tag="ON430_000393"
     CDS             33381..34775
                     /locus_tag="ON430_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR3218341.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT         9666 a         7315 c         6214 g        11581 t
ORIGIN      
        1 aaaagggact gagacaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat
       61 catctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg
      121 tttctctaat actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga
      181 agtagcttct tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa
      241 attccttctt atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa
      301 acacggataa acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt
      361 gctcggtgca acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct
      421 tcttcgattt ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca
      481 cgttcacgtt ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt
      541 ccgccattaa cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact
      601 tcatcaatcg tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca
      661 gctgtcgtac gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt
      721 accacattta ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt
      781 cgctttttct acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc
      841 atcgtatttg ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata
      901 tgaacctgga agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg
      961 gactctgcgg gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa
     1021 gatagcaatg atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc
     1081 tactttataa tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa
     1141 tggatctacc gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc
     1201 atccaaatgg gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata
     1261 gattgcttgg atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg
     1321 acccatttct gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa
     1381 ggctgatact tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac
     1441 atcttgacct tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg
     1501 catacgtgca cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac
     1561 tcctgaatct ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc
     1621 ggtaaataca gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag
     1681 tacggtttta ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg
     1741 ggcaagcaaa tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc
     1801 gtcaaaggct ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt
     1861 ttctaaatcg atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac
     1921 aggaacagag attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt
     1981 tgattccatc gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac
     2041 aacttttgat ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa
     2101 ggattggtct aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc
     2161 tgaactcatc ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa
     2221 tttcggtaat ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct
     2281 catcaatgat attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat
     2341 gttctgctgt tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca
     2401 ataactgaga caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag
     2461 ttgcttcttc tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag
     2521 ttagcgaatt gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt
     2581 acatagttgt tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata
     2641 gattgcgcag ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc
     2701 cgccaattgc gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca
     2761 tcgtttgttt caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga
     2821 cgatataacc cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc
     2881 ctctaggatt ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt
     2941 ctcttacttt tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact
     3001 tagaagcaga caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga
     3061 tacgtgtctt gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga
     3121 atggatttca atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct
     3181 ttatattcct gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg
     3241 attgtttcaa agatatctga atgattggta tccagatatt cgaataattc atgttcgaag
     3301 tctaaaatac tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc
     3361 actacttgtt tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta
     3421 cggcgtccac gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta
     3481 aatgcttcta attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc
     3541 attgctttga tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct
     3601 ggacgagtac ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa
     3661 ataacgtttg ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag
     3721 gcagtcattg aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt
     3781 gagtgtaagt agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc
     3841 aaggaaagtt cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca
     3901 tgtttcccgt tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa
     3961 agcaacggcg ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca
     4021 tattttttca atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca
     4081 tagatacaga tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt
     4141 ttccctgttt tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga
     4201 gcgtcgattg cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg
     4261 cctggtgcca ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca
     4321 attggctgac ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc
     4381 atgatctttc ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg
     4441 ataatgatac ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa
     4501 aattcaagca actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca
     4561 cctacataag ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt
     4621 tgttctttaa tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc
     4681 tttgtactat ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc
     4741 gattactcga tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg
     4801 ttttaattcg actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg
     4861 ttgttcgtct gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc
     4921 atcatatcga cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc
     4981 ataaacaact tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat
     5041 gatttctcgt ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg
     5101 tacttgttca tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt
     5161 tgaatcgatc gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc
     5221 tagtttcatt tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt
     5281 ctggagataa ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat
     5341 cgtcttttac agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac
     5401 tttttagacg agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc
     5461 cactttcttt cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt
     5521 gttcgcgttc ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat
     5581 cattggcgat cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat
     5641 gcttcaagag agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca
     5701 tcgcattgcc aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt
     5761 gaagtacttc tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat
     5821 actgattcaa ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa
     5881 cagcttcgac tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag
     5941 gttggcgagc cattgattcg attgttttag agataacttg accgttacca taaccggctc
     6001 cgattgctgc tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc
     6061 taaattagat aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta
     6121 tggctcatat aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac
     6181 aaggagaatg cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct
     6241 aagcttatta ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca
     6301 taaagacgta aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac
     6361 ataaaactat atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc
     6421 accccaaata agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg
     6481 tctgctgtcg ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa
     6541 ccgatgttgt ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg
     6601 ctcaattctt ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat
     6661 tctatcgcat tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct
     6721 aagaagaaaa caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa
     6781 attggtccaa tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg
     6841 aaattcacat aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac
     6901 acggtatcat accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt
     6961 ttttatttca gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga
     7021 aaaaacctat gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta
     7081 aaagcaaaag caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa
     7141 tgttcgatca atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga
     7201 ttttttccct ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt
     7261 taaaggaaca acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt
     7321 tttgtgtaat aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc
     7381 ataaggactg atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc
     7441 atcttttaga ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat
     7501 ccctgcttcc atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat
     7561 taattttccc tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt
     7621 cttcttttta acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt
     7681 actatcgcta tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt
     7741 tccttcgcgt tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc
     7801 ttctgcggaa acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt
     7861 tactcgcact ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa
     7921 atgattttca acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc
     7981 tactgtattc ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc
     8041 gaattcttcc cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg
     8101 ttctgtttct actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc
     8161 cgtagcccac gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg
     8221 atggacgatt agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc
     8281 cgctaagcca taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag
     8341 caacatagta ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg
     8401 taaatctttt ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc
     8461 aaaatcaaag aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa
     8521 cggcagattt aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga
     8581 ttcgatcaag cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc
     8641 agcatcaaca aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg
     8701 catcgtttca aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc
     8761 atagcgttgc atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt
     8821 catccgttct gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt
     8881 tggatcaatc tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca
     8941 gataccattc gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact
     9001 tgtccctctt tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac
     9061 atctgcaatg tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc
     9121 atcatctaga tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc
     9181 tctacgtcca gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa
     9241 tacatcatca ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat
     9301 gccagggtca ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata
     9361 ccctttttca gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac
     9421 tccctctgct gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac
     9481 gccgtaaaga tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa
     9541 ctgagtagtc gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc
     9601 tgtatttgct cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt
     9661 cgcttctttt gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc
     9721 tcgttcattt gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt
     9781 tcctttttta ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag
     9841 taatttaaaa tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa
     9901 actgcgtttg cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt
     9961 catatttatt cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat
    10021 tcgcgtcttg ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc
    10081 actcgctgac ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca
    10141 tctttcccag cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg
    10201 attgctgcct gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact
    10261 gctttttcat tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat
    10321 ttttcaaagt tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg
    10381 ccgccaatca ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat
    10441 cctaatacgg caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg
    10501 tctgcccacc agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt
    10561 cctttaaaaa gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt
    10621 cgaacatcgt ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt
    10681 tttgcataaa ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac
    10741 catagtttct ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc
    10801 cgttttgcga gaggcttatt caactacttc gaagataaat atgctaggac aaataataag
    10861 atcatccaga tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga
    10921 gctttttgtt tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt
    10981 tgcttgcttg gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt
    11041 cccaaaatta aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt
    11101 tatcatagat aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa
    11161 actttcagct gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca
    11221 aactcttttt ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat
    11281 tctactccag tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca
    11341 aatgtatggt caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc
    11401 tcttgttgtt catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc
    11461 cgcactcctc aaataactga tattttttta ttcctacctt attttagcag tctcctgatc
    11521 tataaacaat tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt
    11581 tattctttgt tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga
    11641 tgcttccaag tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt
    11701 ttgaatcgtg tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa
    11761 taaaggcaga tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc
    11821 ggcgtatttc tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt
    11881 gattttactt aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat
    11941 aaatcctttg accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc
    12001 cattgatccg ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat
    12061 ccaggcaatt aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc
    12121 tccgtgcgca cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc
    12181 tatatgagac atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt
    12241 attctgctta tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt
    12301 gattgttttg ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg
    12361 ttttttttct actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata
    12421 ccagtcatat cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag
    12481 aaaatggtaa ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt
    12541 acacccgccg ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg
    12601 attctttatt ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag
    12661 ttctcctcaa tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac
    12721 taatttcgtg cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat
    12781 aaaaaactaa gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat
    12841 tgcgatccag ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc
    12901 gaagtagttg ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat
    12961 gtgctgaaat gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa
    13021 aaacctttga agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg
    13081 gaaacaaatt tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt
    13141 ttggctgatt atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc
    13201 aatatcgctt ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc
    13261 tcgatgggtg gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac
    13321 tggtgtgatc ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg
    13381 gttgggttag atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt
    13441 caaatgaatc ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc
    13501 cactggaaac aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat
    13561 tttatcgagg gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga
    13621 ataagtcgtg gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt
    13681 aaaattatga caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta
    13741 aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat
    13801 gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa
    13861 ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg
    13921 aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag
    13981 cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat
    14041 tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag
    14101 cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat
    14161 cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct
    14221 attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga
    14281 ctaccttgat cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca
    14341 acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata
    14401 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
    14461 taattccttg atatttttca atcatatcca aataattatc gatttcaaca tactcatcta
    14521 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
    14581 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
    14641 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
    14701 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
    14761 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
    14821 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
    14881 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
    14941 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
    15001 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
    15061 gcgtatacat caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta
    15121 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
    15181 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
    15241 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
    15301 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
    15361 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
    15421 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
    15481 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
    15541 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
    15601 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
    15661 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
    15721 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
    15781 gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa
    15841 acatcatata cagaagctag ttgcaaaaaa aactctctac ctgctgatat ttcagcaagt
    15901 agagagttcg attacttaac taagtgatca tactcgacta tctttcaata gtcgatcatg
    15961 tatcttcatt agaaaagaat tatttgtttt ttaagttgta gaaagatttc aaacctttgt
    16021 attctgcaac ttcaccaagt tggtcttcga tacgtagtaa ttggttgtat ttagcaatac
    16081 ggtcagtacg tgatagagaa ccagttttga tttggccagc gtttgttgca acagcgatat
    16141 cagagattgt tgaatcttct gtttcaccag aacggtgaga tacaactgct gtgtagccag
    16201 cttctttagc catttcgata gcttcgaaag tttctgttaa tgtaccgatt tggttcactt
    16261 tgataaggat tgagttagca attccttttt cgataccttc agataatttt tgagtgtttg
    16321 ttacgaacaa gtcgtcacca actaattgaa ctttgtcgcc taatacgtca gttaatttct
    16381 tgaatccgtc ccagtcgttt tcgtctaatc cgtcttcgat tgagatgatt ggatatttag
    16441 aaactaattc ttcgtagaat ttgatcattt cgtcagttgt tttttcgcct tcgcctgaat
    16501 cagctagtac gtaaacacct ttttctttgt cgtagaattc tgaagaagca gcatccatag
    16561 caagaacaac gtctttacca ggtacatagc cagctttttc gattgcttcg atgattactt
    16621 caaaaccttc ttcgtttgaa ccaaggtttg gagcaaatcc accttcgtca ccaacagaag
    16681 ttgctaatcc gcgagctttc aagattgaag ctaatgcgtg gaatacttca gcacccatac
    16741 gtagagcttc tttgaatgtt ggagcgccaa caggcatgat catgaattct tggaagtcga
    16801 ttgagttatc agcatgtgat ccgccgttga tgatgttcat cattggtgtt ggcaatactt
    16861 ttgtgttgaa tccgcctagg tagtggtata aaggtacttc taggtagtca gcagctgcac
    16921 gtgcaacagc gatagaaaca cctaaaatag cgttcgcacc taatttacct ttgttaggag
    16981 ttccgtctaa agcgatcata gctttgtcga tagccatttg gtcacgaaca tcgtagccaa
    17041 tgattgcttc agcaatgatg ttgtttacgt tgtcgacagc tttagttaca cctttaccgc
    17101 cataacgagc tttgtctccg tcacgtaatt caactgcttc gtattcacca gttgaagcac
    17161 ctgaaggaac cattccgcgg ccaaaagctc cgctttctgt gtatacttct acttcgattg
    17221 ttgggttacc acgtgagtct aagatctcgc gagcgtaaac atcagtaata attgacatgt
    17281 tttgtctctc ctttgagttt gttttttcct aagggatttc cctcatatac gattttagtt
    17341 aattttgctc attagtgcaa acatttcttg catttttcgc aaattttcaa caattatttt
    17401 acagcgtcta acaatgacaa gaatgaatct gcttgcaagc ttgcgccacc gactaaagca
    17461 ccgtcaacat tttcttttgc catgtattca gcaatatttt ctggttttac agaaccgccg
    17521 tattggatac gaactgcttc tgatacttct tttccataaa gtttttcaac tgtttgacgt
    17581 acaactccac aaatttcgtc agcgatttgt gcgtcagctg atttaccagt accgattgcc
    17641 cagattggtt cataagcaat aaccatgttt gacacttgtt cagctgaaag atcagctaaa
    17701 ccagctgtaa tttgtccttc gatccattca gcagttttgc ctgcttcata ggtttctaaa
    17761 gattcgccac agcataggat tggtgtcata ccatttgcaa agatagcttt cgctttttta
    17821 ttgatgtctt cgtctgtttc atggaaatat tcacgacgtt ctgaatgtcc gatgatcaca
    17881 taatgaacac ctagatcagc aagagctgct ggtgaagttt caccagtgaa cgcacctgag
    17941 ttttcccagt agcagttttg tgctgagatt tttagttctg taccttcagc tgatgtcatt
    18001 aattcttgta agaataatgc aggagatccg atgactgaat caactttgtc atttgaaggg
    18061 attttgtttt ttactgcttc agcaaattct tttgcttctg atgcagtttt gttcattttc
    18121 cagttacctg cgataatagg tttacgcatt gaggaacaca ctccttaatt tattgaaaaa
    18181 tggctaggga gtcatgaaaa ctcccttcac cttttgatcg attactcgtc agaattgagc
    18241 aaacttggtc gtttatttat cgttgattgc tgctagtcct ggtaattctt taccttcaag
    18301 caattccaag cttgcgccgc cacctgttga gatgtgtgtg aatttgtcag caaatcctaa
    18361 ttgttctgct gctgcggctg agtctccgcc accgatgatt gttgttgcgc cttctaagtt
    18421 agcgattgct tcacaaacac cgattgtacc tttagcaaag ttactcattt caaatacacc
    18481 cataggtccg ttccaaacaa ctgtttttgc accttgtaat gtatcagcaa ataattttac
    18541 tgatgctggg ccgatgtcta aagccatcaa accatcagga atgtctcctt cgattacttg
    18601 tgtttcaaca tcatttgaaa attctggtgc acagacattg tcgattggca agacaagttt
    18661 gtcgcctgct ttttcgatta attcttttgc tagttccact ttatcagctt ctactagaga
    18721 gttaccgatc ttgattcctt ttgcttcgta gaatgtgtaa gtcattccgc caccgatcaa
    18781 gattttatct gctttaggaa tcaagttttc gattacgccg attttatcag aaacttttgc
    18841 gccaccaagg attgcaatca ttggtcgttt tggttcttct actgcttcac cgatgaactt
    18901 gatttctttt tccatcaaga atccagcaac tgttgggata ccagttgaag cgatacctac
    18961 gttagaagca tgtgcacggt gagctgtacc gaaagcatcg ttaacaaata catcgcctaa
    19021 tgaagcccag tatttaccta gttctgtatc atttttgctt tcttttttac cgtcaatatc
    19081 ttcaaatcgt gtattttcga atacgacaac gtcgccgtca ttcatgttgc ggattgcttc
    19141 ttctaattgt tctccgcgag tttcaggtac gaatgttact tctttaccta ataattcgcc
    19201 tagacgtttt gcaactggag ctaaagattt tccttcttta tcttcttctg tttttacacg
    19261 tccaaggtga gagaaaagga ttgcttttcc gccttgttct agaacgtagt tgattgttgg
    19321 caatgctgct ttgatacgag tgtcgtcagt gatcacgccg tctttcaaag gaacgttgaa
    19381 gtcaacacgg acaagaactt ttttgtcttt taaatcgata tctttcactg ttttcttagc
    19441 cattttattt cctcctgaat aaacttatta tcaaaaaaag cggaagaagc gcgattgctt
    19501 ccccgctttt aaatgtacat gtctataatt tacacttgta cgtttgtcta tcttataagt
    19561 tagcgaagta ttctaaagta cgtactaatt gtgcagtgta tgacatttcg ttgtcgtacc
    19621 aagcaacagt tttaactaat tgtttgtcgc caactgtcat tactttagtt tgtgtagcat
    19681 caaataatga accgtaagtc ataccaacga tatctgaaga aacgatttcg tctgtgttgt
    19741 atccgtaaga ttcgtttgaa gcttctttca ttgctgcatt gatttcgtca acagttactt
    19801 ctttttcaag aactgttact aattctgtta atgaacctgt tggtacagga acacgttgag
    19861 cagctccgtc taatttaccg ttcaattcag gaattactaa accgatagct ttagcagcac
    19921 ctgttgagtt aggaacgatg tttgcagcag cagcgcgtgc acggcggaag tcacctttag
    19981 gatgaggtcc gtctagagtc atttggtcac ctgtgtaagc gtggatagtt gtcattaatc
    20041 cttcaacaac accaaatttg tcgttcaatg ttttagccat aggagctaaa cagttagtag
    20101 tacatgaagc acctgagata actgtttctt taccagttaa tgtttcgtgg tttgtgttat
    20161 aaacgattgt tggaacatcg tttccgccag gagcagagat aacaacgcgt ttagcaccag
    20221 ctgttaaatg tttttcagct gctgatttag aagtgaagaa accagtacat tctagaacga
    20281 tatctacgcc tagttcgccc catggtaatt cttctgggtt gcggttagct aatactttaa
    20341 cttcttttcc gtttacgttg aatgaacctt catgaacttc aactgttccg ttgaaacgtc
    20401 cttgagttgt gtcgtatttt aacaagtgtg ccaacatttt agcatctgtc aagtcgttga
    20461 ttgctactac ttcgattcca tctacatctt ggatacgacg gaatgctaga cgtccaatac
    20521 gtccaaaacc attaatacct actttaactg tcatttaaga tttcctcctt atgaaaatca
    20581 aaaaaatatt tattttaaag ggttacccct tttaaaatct catttgcagc agcttcatca
    20641 gtgatgagcc acgtttgttt tggtgcattt ttcatataag ctcgaattgc tttggctttt
    20701 gattttccgc ccgcgattgc catgatgatc gggacttttt ccaaatcttg caattctatg
    20761 ccgattctag ggaccttata aacgacttca cctttttcat tgaaaaagta gccaaaagat
    20821 tccgcaacag catttgcttg tttcagcatg actaattctt tttcagtcat cttacgacgt
    20881 gctgccatgt gtaatgcacg cccaatacta tgaataacac aattcgcttc actaatcaag
    20941 tttaagactt cttggataga tggctcattg agtaaagaat tgtaagttgc cagacttaat
    21001 tgttccggaa cataaagtgc acggtggtta ccgtttgctt tagcagccat tcttgcactg
    21061 acagaatttg cctgtacagt tatcgcttca ccaatcccgc ctcttgcagg gacaaacagg
    21121 ttatgccgtt tcttattttc cagattagac aattgttcgg ctaccacagc catagtagtt
    21181 cctcccatca cagcgatgat attctcgcct tctggaagta gtcggtctag ggcttcgctc
    21241 agcgcttcgc cgaatgcatc agccactttg gtctgctctt cgctgtttcc tgaaacaacg
    21301 atacagcgtt ggttaccaaa atattctgcc aactgttgtt cggtttgatg cgtacctaaa
    21361 agctgatcca tgaagttttc aaggctttga taaacttctt cgcccttagt tgtcagcgtc
    21421 ataccgcttt ttgaggtgtc gatcaaattc agatttttca aaagatctgt ttctgttcgc
    21481 aatactcgtt ctgtgatccc catactttcc gataaacttc gacgtccaat cggctgcatc
    21541 caatagatat ttcgtaaaat tttatagcgt tcttgcaaca cttcgataat atctggtgca
    21601 acagattcaa tcatttttat ttcatccagc atagcgatct ccttagttgg acactttacg
    21661 tccaatgtag acttaaaacg acccatatac gataaaaaaa gaaattaact attcgaattt
    21721 cacatcaata aagggaaatc tcaacctcac gactgtaagt ctaacagttt tgctctcgtt
    21781 tttcaactaa aaagactaga aaaagtgaaa tactttttct agtcttttga ctgattagtt
    21841 atcataacgt tttctttttg atgaactcgg gattcctaat aaatcacgat attttgctac
    21901 tgttcgtctt gagatcgcta tctcatcttt tttcagcaat tcaaccaatt tctggtcaga
    21961 caacggcttg tttttgtctt cttgatcgac taattcctgc agctttttct tcgctgtatc
    22021 tgctgaaagg tcttctgtct gttctgtact attcgtagga attcgtgtag tgaaaaattt
    22081 ttttaattca aatacaccga agtctgtttc caaatatttc ccgttgactg cacgactgac
    22141 cgtggattca tgcacatcta tctcagttgc aacttccttt aacgtaagag gcttgattgg
    22201 acgttccttg tcgataaaga aagctttttg ccgggaaaca atgacttgag caacccgcaa
    22261 aattgtatcc ccacgttgta ggatcgtttt cttcaaccat tcgaattcct gtttccgttc
    22321 ttttaaataa ttttcagttt ctttatctgc ttgttgtgac atttgtttga agtaactctc
    22381 ttgaaagcgt atatttggct gattttttcg gtttgagata acttgtacat ttttatcgtc
    22441 gtccactaat acccggacat caggaataat gtacaattcg ctgctacggt cgaaaatccg
    22501 gccagggctt ggactcaacg tttgtaaata gtcgaatatc tgctgaacag catgtagatc
    22561 aacagaaaaa cgttgtgcga tttctttcca ttttcgctcc accagttcct caaaacattc
    22621 ttccaacacg atataagcta attccggcgc ataatcatct cgttctgttt gaagcatgag
    22681 acattcttgc aaactacgtg cacctactcc agctggttct aattgctgaa tcagagtcaa
    22741 cgcatcgagc atttggatag gcgtagctcc aatctctttt ccggcttctt ctaaactaat
    22801 cgtcaaaaat ccgttcaaat cgatgtattc tacaagataa agaacgattt tcctcaaaaa
    22861 agtatctcgg taattcaaat ggacttgttc gatcaatgaa tcaaataaag acccttttgt
    22921 atcaggaatc tgatttaggt aatttgtctc ttctccttca taagaagatg aagaagcttt
    22981 tatgtaatct ggctgccatt ccggctcaac aacttccacc aatggatttt ccattgcttg
    23041 attctcaacg aatgccaaca gctcctcact gttatattgt aaaacttgga tggactgttg
    23101 caactgttgc gtcatggcta gtttctgtgt ctgctgctgt ttttgtgaaa aattttgctc
    23161 aaatttcatt tttttgtcca atccccttgt aattttttat ctgtttagtt acaattatcc
    23221 cagtgcttgc gtccttagtg tagtggatat cacgtaagat tccggttctt gagacggggg
    23281 ttcgattccc tcaggacgcg ttctactgct attttagcat aatttacgaa tagttgcttc
    23341 tttattttta agtgaaaact gtccgccaaa gaaaattggt ggacagtttt ttgttgtacc
    23401 tgtatagata ttgttaagcc tttgcaagta aggtagttat gtgttcaact acttattaaa
    23461 atgtgttatt tattaaagat acttgtctca cttactgaac tggccgatga tcgtttctgc
    23521 cataatagtt ctcgacagcc gttggatgcc ctaataaatc cccaactgtc gtgatgcgat
    23581 acccttgctc tttcagataa tcgatcactt gaggaacggc acgaatcgtt tcaggataga
    23641 tatcatggaa taaaataatt gccccatcgt atactgttgc ttggatcttc tgcaaaatca
    23701 aatcaggatt tcttgtcttc caatcttctg aatcaacaga ccattcgatg atcggtcgct
    23761 ggatgatcgt tgctcctaca ttcgtgacag aaccataggg cggacgaaca aaagtggggg
    23821 ttttgccagt gacttttttg atctcagtgt ctgctttcgt cacttcctcc gtcatcgact
    23881 gggcagaaag agtggccaga ttctgatgat cgtatgtgtg attgccaatc tcgtgccctg
    23941 cttcagctgt ctgcttcacg atttcaggat gctgcgccgt attttctcct aagacgaaaa
    24001 aagtggcatg cacgtttttt tcttttaaga ttcgcagaag ctgcggtgtt gtggtcgctc
    24061 ctggtccatc atcaaagctg attgcgacgc ttttgacagc cggatctcgg ctcgtttttg
    24121 tactgtcttg tgttgattgt gatgactgac tttgtacctt ttccgctgat tcttgaatgg
    24181 cttctttgac gttagctgtt tcagttgatt ggcttttaat ggttgattgt tctgattgtt
    24241 tgaaagattg tgacgaatgc tccgaagagt gtgtttgtcc ttttacttcg gtgatcaagg
    24301 aggccaccga agtaatttcg aaaaacaaaa gcacggataa agcaataata ctgataacta
    24361 aataatttct tttcactata tctcttcttt ctctttttct cttcttatta tttacttgta
    24421 cttacttgaa aattgaaact gctagtcgca actacttctc gtctaatgat ccaaataatc
    24481 ttccgatgcc aaaatcgttt ttgtcacgga cttaggaatg acaaattctg gtgtacccat
    24541 ataaccagga gcaatttcgt attgagaaaa agtcaaaact aattgatgct ctttgttcaa
    24601 ataaaaactt cgtgtaggcg taacaagtgt tggtttttct atcgtccctt cttgaatatc
    24661 ttgctcagtc caatagtact tagatggatc ttccttgact tgttcggcca tctgccgttt
    24721 gacttctttt gtcaaaacag atagataggt gtcatctttg aataaaagtg gaagtgagag
    24781 ctggacactg tttttcttgt cgattgtgtc gtatcttttt tcaacatggc tatctgctgc
    24841 tgtttgggtg atctgccttt caatcacgag aaaccggtta tcgtctacga ttttctgata
    24901 atctcccaaa acttggtaat gatccgtttc aaaatttcca tattcttttt cagctttttc
    24961 aaaatctgct tgtccttcgc gaaagtattt ttgatttaat ccatgaattg ttttattttc
    25021 tttctcattt tgtgtattga tcttcggtac ctgaatatcg attttttcag atcgatcagt
    25081 aaagcgttcg cctaagatca attcaatgac tgatcctaag atgggtaaat cttctgcaaa
    25141 gcttcgaact tgactattaa aaagaaggct acttgttgta atcattacga tagccaaaga
    25201 tactccgaac ccttggagac gtctctttct ccgctttgct tttataaatg attgctcttc
    25261 gataataaaa cgctggcgga tctcctcttt aagccctttg ggaattgctt gttctttata
    25321 agttttcttt agttttttta aattttcttt ttgggaatac atggcactct tctctccttc
    25381 attctaggat ttgacgtaaa gcattcaatg agcggtacat tttcgtttta actgtgtttt
    25441 cattcatatc caagatagtc gcaatttctt tcaaacgaaa tccttcaaaa aagtagagga
    25501 tcagaatctc tttttccgga ctgtctattt tatctaaaat catatggagc tctgcaacgg
    25561 aagtttctaa aggtaactgt tgggggatat cttgaatttc aaataagtcc acaggcgtcc
    25621 ttttttgtct gcgccaacta tctaatgccg tgcggatcaa tatccgataa aaccatgcac
    25681 taaaatgctc gatctctttt tctttttcaa aatagttcag ggctttatga aaactatctt
    25741 gcatcacatc taaggcctct tctttatcat gtacgtaatt catcgtgata cggaacattt
    25801 tttgataatc agtttcgatg agtccattca gcaggtgacg gtatttttcg atctgccgtt
    25861 ttttcttata tccagtaaaa atcattctcc actcctttct gcctcactta atatgacgta
    25921 caaggtgcgc taaaagtttc aattttttta ttttttatgt atttacttgg gatttttata
    25981 tacaaaaaag aaggtgtgac aaaaatcgtg tcacacctcc ttattctgat aggctgatcg
    26041 tctctgtcac aacctctcgt aagaaaataa tgtttattct ttttcagagt cagaaaattc
    26101 ttcctcttca taatcttcat catcatcgtc ttcgtctatg atacttaaat cttcttcgat
    26161 atttcctggc aagtcttctt catcatctgt cgggtcatct gctccgattt cttgcaagtc
    26221 agaattataa gcagcgattt cttcatcttc atcatcatcg tcatcaaaca gatcatcgtc
    26281 atcttcttct gtcagatctg tatcttctgg gtcatcgtca ttgtaatcga tcgcgtcttc
    26341 atcatcattg ataaaggcgt tgactttctt gcgtttacgg cgacgtggag tatcttcatc
    26401 ctcttcgtcc attccatggt ttacttcttc atcgatcgaa tcgatagcat accatgaacg
    26461 caagccccaa cgattatccc ctaatgagat aaagctgccg tcaatattca agtcagtata
    26521 aaattgtgcg agtgaatcac gaatttcact gtctgatttc ccaagatagt tttggatctg
    26581 gttagctaaa tcagaaaaat ccatgacatc gccacgttgt tctaagatcg cgtgtgctac
    26641 ttcgatcatt gataattcgc ttttgtttgc accttcaaat acactaattt ccaatcagga
    26701 cacgtccttt cacagtctac tccttatcat acaaaatcag acacaaaaaa tcaaactttt
    26761 cttttatatt tacgctgtaa aatccaaggt caaataatat tcgccgattt tttccggtcc
    26821 catgaacaga tcataatcga ttcgaacctt ccctcctgtg ggacggtctt tcaaactaac
    26881 atgcagttct ttcgtaaaag tagcaatctg gatcataccg tatggtgttc gataggatgt
    26941 ttctaaccgt tctttatagc caaatttcaa acgcatccgc atttctcctg cacggatcag
    27001 ctgtacatga ccatctggaa ctaatttgat cgttacagga atctcatcgc ccgtctcctc
    27061 ctgaatctct ttataacgta tgtacagcgt atcgccaatt gtcaccattt gccctttcaa
    27121 atcaaaataa aaatcttgca cttccccttg ctgttttact tttgtacgca atttgattga
    27181 aatcggtgtt ccttgtgata attccattat tcattctcct ttacttctca tagctacatt
    27241 gttcctagtt tcttttgttt tgtcaaccaa cttgtccatt tttacagaaa accttttttc
    27301 caaaacaact ctttgatttt agagtataat attgtagaaa atatgaattt atacaaagga
    27361 gccggttgaa tgaaccctgc gattcgacta gatcagcaca tttatcaaaa aaacgacttt
    27421 tccccttttg cattaacaga tatattaacg gaatattcta aagaacacca gcaaatcttt
    27481 ttacatttct ggcaatatga ctgcacagtc atcttaggta tgaaggatgt tcgaactcct
    27541 tttctaagtg acgggatcca aacactgaaa gatgcaggtt acgctccagt gatcagaaat
    27601 tcaggtggat tagggatcgt atcagatgaa ggaattttga atatttcttt gattttcccc
    27661 caaagtgaca caaaaaaaat aaccatagat gacgcttacg agcaaatgct cctattgact
    27721 aaatacgctt ttccaaaggc accgatacaa gcattcgaaa tcacccattc ttattgtcct
    27781 ggaacttatg atttaagcat ccatggcaaa aaatttgccg gtatcgctca gcgtcggatc
    27841 aaaaatggga tttctgttat gatgtactta agtgtcaacg gtaatcagga aaaacgcggg
    27901 caagtcatga agcgatttta tcaatctgct ttaaaagaag actttggtac aaatggctat
    27961 cctgcagtgg atcctgctac aatggcaaca cttgaagaac tgcttgacca accccttttt
    28021 gtaaaagaag tcaaagaagc atttgcaaaa gcttttgaaa agctgtattc tgcaagtgat
    28081 ttacaggatt ctaccgactg gctaaaaaaa gaaatccagc tggagcaatg gaacaaccaa
    28141 atccagagaa tggaacaacg taataacata aaggagctac attatgacaa ccccttataa
    28201 atatcaaatg ctgcctatgg aaaaagtttt tcgtgatcca gtccataact atatccatgt
    28261 ccagcatcaa gtaatcttag atttgatcaa ttcgaaagaa gtccaacgtc tacgacgcat
    28321 taaacaatta ggcacttctt ctttcacttt tcacggagca gaacatagcc gttttactca
    28381 ttcattaggc gtttatgaga tttcaagacg aatctgcgat atcttttctc gaaatttttc
    28441 aaaagaaaaa ataggcaacg gaggctggga tgatcaggaa cggctggtga ctttatgtgc
    28501 ggcactgttg catgatgtcg gtcatggtcc ttactctcat acatttgaac atatcttcca
    28561 tacgaatcat gaagccatca ctgtagagat catcacttct ccagaaacag aagtgtatca
    28621 gattcttaac cgagtagaag aaggttttcc tgaaaaggta gctagtgtga tccaaaaaac
    28681 ttaccccaac ccacaagttg tccaaatgat ttctagtcag atcgatgcag atcgaatgga
    28741 ctatttgctt cgggatgctt atttcactgg aacagaatat ggaacatttg atcttacccg
    28801 aatattacgt gtgattcgtc cttatgaagg cgggatcgct ttttccatga gtggcatgca
    28861 tgcagtggaa gattatatcg ttagccgata tcaaatgtat gtgcaagtgt acttccatcc
    28921 tgtttcccgt ggtatggaag taattttgga gcatctgctt catcgcgcac acgagttgta
    28981 tcaagaagat gccagcagtt ttgaactcca ttctcaatta cttctgccat ttcttgacgg
    29041 aagctttact ttagaagaat acctcaaact tgatgatggg gtcttgggca cttactttac
    29101 tcaatggtct gaatctgctg atcctatttt aaatgattta gctaaacgtt ttttgaatcg
    29161 taaaccactg aaatctgcta ctttttctgg caaccgggat tcaaaattag tccaagaact
    29221 cactttgtta gtagaaaagg tcggatataa tcctgtttat tacactgctg tgaattctag
    29281 ctacgatcta ccttatgatt tttatcgccc agaacaaggc agacatcgca cacaaatcga
    29341 gattttgcga aatgacggaa ctttgataga attatcacag gttagtcagc ttgttgctgc
    29401 attagcggga caagaacaag gagatgagcg atttttcttt ccaaaagaaa tggttgatcc
    29461 gtccttgcgt gatcattatg atttatttga cgaaacttat caagaatttg ccagccatat
    29521 ccgtaatggc gcattgattg aaataaatta aaatatattg gaggaaatta tgtctatcaa
    29581 actagtcgct atcgacatcg atggtacttt attaaatgag aaaagagaag taacaaaaga
    29641 agtaaaagaa gcgattgccg ctgctgttgc aaaaggtgtt tcgatcgttt tatgcactgg
    29701 ccgtccctta ccaggtgtcc aggaacagtt aaacgagttg aacctttttc aagataatga
    29761 ttacgttatt acttataacg gggcactcgt acaacaaacg aaatcaggta aaatcatcgc
    29821 ccgtcacgga ctgacacatg aagatttcct agagatcgaa gtgatggcac gccgtgtcgg
    29881 cagtcatctc cattcgatcg atgacaaagc gatctacacg cctaatcgag atatcagcgc
    29941 ttatacgatc cacgaagcaa gtttagtcaa gatgccgcta aaatatcgaa cacctgaaga
    30001 aatgacacca gacatgaaca tcgtcaaaat gatgatgatc gacgaaccag aaattttaga
    30061 tgcagcaatt gcgcgtttac cgcaaacttt ccgtgataaa tacacaacgg tcaaaagtgc
    30121 gccctattac ttcgaagtac taaataaaga agcaagtaaa ggtgcggcag ttgcgaatct
    30181 cgcacagcac cttggtattg atcaagatga aatcatggct atcggagata acgagaacga
    30241 cttatccatg attgaatatg ctggtctagg agtagcaatg ggaaatgccg tttctcttgt
    30301 caaagaagca gcaaatgtca tcacttcatc aaacgacgag cacggtgtag ctgaagcaat
    30361 caaaaaatat gtactgtaat aaataaaaaa actgagagaa agctgatcta ctcgctttct
    30421 ttcagttttt atttttttcg atccatctct cgactgccca tcggtggcta ccacgtttca
    30481 atttcacaag cgcttcttga gggctcaccc accaaatttt attggtcttt tcagttggtt
    30541 ctcctatacg cttccaagaa tcagcaacaa agaaatatcc tggattataa taataagttg
    30601 cacgataatt cgagtaaaaa tactcatcag cttctcccag ataagaagaa atttctgcca
    30661 caattcccat ttcttctatc agctcacgga caatcgcttc ttctttcgtc tcttctcctt
    30721 caatttcccc gccaggaaga aaaaaagcac catttggagc ctcgattact gcaatctgct
    30781 gatctctttc tatgacaata tatgctgcat aacgggtttg ataatcagca aattgctttt
    30841 tttcaccaaa aattggttta tccatgaata gtttccacct taacagtttt attttctatc
    30901 cattttacat gagaaatttc tcatgaacaa gcacaaatca actatttcct tacttctttt
    30961 tgttatgatt gagtaaaagg aaaaggagag atgaatctat gaaaatggct cacacatgtg
    31021 ttcgagtgaa agatcttgaa gcatcaatcg aattttatca aaaggcattt ggttttgaag
    31081 aaagccgccg ccgtgatttt ccagaaaaca aatttacact tgtttatttg acacttcctg
    31141 gggatgacta tgaattagag ttgacttaca actatgatca tggtgcgtat gatctaggag
    31201 acggctatgg tcatatcgct atagcagctg atgatctaga aaaattacac gagcaacata
    31261 aagctgccgg tcttgagatt actgacttaa aaggattacc tggcactgcg ccttcttact
    31321 actttgtcat tgaccctgat ggttataaaa tcgaagtcat ccgaggatag ttagtatggg
    31381 gcattttact cttgattgaa taaaaaacaa acaccaataa ttaaaatgag acgcaaactt
    31441 ttcattaaat tgaaaaagtt tgcgtctttt tttaacaacc attgtagcta taaggttgcc
    31501 actctccacc aatttatttt taattcattt gaaagaaatc aaataaacga ggtaccttta
    31561 gaaaacaaaa ctgcttttcc tcctattatc acacgatctt cttttaattc acattctaga
    31621 tccccgcccc ttgatgacat ttgataagct gacaattttg ttttattcat tctttctgcc
    31681 caataaggaa taagattaga atgtgctgat ccacatactg gatcttcatt gattgttaat
    31741 tttggaaaga aggttctaga aacaaagtct ttttgcacac tttctgctgt cacgatgact
    31801 cctattccta attcaaaatt tttgatagct gtaaaatctg gctgcaattt agccaccgtt
    31861 ttttcatctt ctaatacgaa aaataaatca cgtgctaagt aggcttcttt aatcttagcc
    31921 ccaatcgctt cttcatattc agacaatatc ggcacttttt tcggcataat actaggaaaa
    31981 tccatgtaat aataatcttt aattcttgtc acaaatagat taccgctttg agtagaaaat
    32041 ttaatcgttt catcaggtat tttgtaatag ttaaataaaa caaatgctgt tgctaatgtc
    32101 gcgtgaccac aaagatcgat ttctcgatct ggcgtaaacc atctaagctc aaattcttgg
    32161 tttttcttca ctgtaaatgc tgtttcagat aaattgtttt caatggcaat tttttgcatc
    32221 gtaccttctg gcagccattc ttctagcaca taaaccgctg ctggatttcc tttgaacacc
    32281 tcatctgcaa atgcatcaac tacgtaataa tctaagttca ttttaaagcc cctttctcta
    32341 atttgttata aatatattct gcacaacaaa gatcttcaat agctagacca actgcgtcga
    32401 aaatcgtcgg cctcgattct cctttataaa atgttttttt ctcagaaact aattcgccta
    32461 atgtaccaac aatacaagat tcctctaaat ctccttctgc catggctata agcaggtccc
    32521 cgctttctgt taaggcagct gagtaatcat ctacatagat ttgagaagtt tccatcaacg
    32581 atgtacttat ctctcttgta tcaggagtaa atgtgcccac agcattgata tgtgcgtctt
    32641 cccttaccca ctctttttga agaaatgcct ctttactaga ggtcaacgta caaatgattt
    32701 ccgctggacg tactgcatct tctactgata ttgcatttat aaacgttatt ttaggatact
    32761 gttttttagc tttagcgata aaagtatttc gtctttcttc taaaatatcg taaacaaaca
    32821 ctgtagtgat gggacgaatg gtagaaatcg cagtcaaatg cgacaatgct tgttgaccag
    32881 ctccaattaa agcaagtgtt tctgcatgcg gatttgctaa gtaatctgtt gccagcgcag
    32941 atacagcagc tgttcgaatc caggtgactg catttgcgtt tatcaatgct ttgggtagtc
    33001 cgttttgagc atcaaacaag aaaatctctc ctaaatgaga aggtaaatga tactgatgat
    33061 tatccggaaa agcggtaatg acttttgtgc caaaatatct gttctccccc aaataagcag
    33121 gcatcatcgc aaacatattt ttgccttttc cgtcaggtaa aatttccacc aatcgaggat
    33181 tttggctgat tttaccactt tcataatcaa aaaaacattt tttcattact tgaatcgctt
    33241 cttcaaaact aaaagaagct actacttcct caaaattaat aattttcata gacaattccc
    33301 tcctcacaaa agattaactt ctaaaatcaa tacaatcaat ggtaaaattg tatggtatac
    33361 aattttaagg agtggagaaa atgcctatta attcttatga caattacccg ttaacttggc
    33421 gaccagacaa acaaaaattg aagcgtcctt attatcgatc gttgattcaa caattagaac
    33481 aagatatttt aaagaataaa ttacagaaaa acactcagtt accctcccaa agagagctag
    33541 ctgattattt agatttaaat tttactaccg ttggcaaggc ttataaatat ggaattgaga
    33601 aaggtttgct gtatacgaat attggaagtg gcacatttat ttctcccaat gtctttgagt
    33661 ccatcaccat ctctaccaat gaagtaaccg aacatatcat tgattttggt ctggttagtt
    33721 cttttgaaga atgtaataaa ttcgttgtcc cgtttattca atcaactagt aataacccaa
    33781 atcttgttgg tttgcttaat taccaagatc ccaaaggcac aaattttcaa ttgactactg
    33841 cagcaaattg gttaaaaact caaggagtca ttccgtcctt agaatctcta tctattgttt
    33901 ccggtgttca aaatggattg gctatcacac tttctgcact tttctctcca ggtgatagaa
    33961 tagcaacaga ccgatataca tacgctaatt tcattgaatt ggctcagtta tatcatttag
    34021 agatcgttcc aattgatttt gacgagcaag gaatgcgacc tgatatattg attcaggaat
    34081 gtagaaaaaa gaagattcat ggtgtctttc ttatgccttc ttgtaataat cctatagggt
    34141 ttcaaatctc tcacaaaaga cgagagcaat tagccgaagt gattgaaaaa gaaggtctat
    34201 gggttattga agatgatatc cattcatttt taacaactta ctatcaacat acgatcattc
    34261 ctcctttcca aaatttatta cctaaccaaa caatctactt agctggaatg acaaaattta
    34321 tttgttctgg attgcgagtt gcttatcttg tagttccagt acatgtccaa agcaagatag
    34381 aacaagcaat ttttaatata aatgtgaaaa cttctggatt agatgtggaa gtcatcactc
    34441 aaatcctcca ttcaaatgaa gcaaatagga tcttagaaac taaattaagt cttacaaaaa
    34501 gagccaatca gctttttgat tctcttttca aagtagaaaa accaacgaat cgtgtccctt
    34561 actatcgaat tcttccagtt gatcctgaga ttgaccctaa gataattgag aatgactttc
    34621 ttgctaacgg tatccgactt tatcattcca gacgctttac tacacgtgcg caaccagatg
    34681 cattcgttcg aatttcccta tcctctaata accttgaaat tttagcttct ggactaagta
    34741 tcgtaaaaga aaaaataaaa aaatatgaat tataac
//
LOCUS       DAJSMI010000009        34656 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJSMI010000009 DAJSMI010000000
VERSION     DAJSMI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.009"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(313..2205)
                     /locus_tag="ON430_000394"
     CDS             complement(313..2205)
                     /locus_tag="ON430_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218342.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="ON430_000395"
     CDS             complement(2202..3929)
                     /locus_tag="ON430_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218343.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="ON430_000396"
     CDS             complement(3945..4595)
                     /locus_tag="ON430_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR3218344.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="ON430_000397"
     CDS             4799..5095
                     /locus_tag="ON430_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3218345.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="ON430_000398"
     CDS             5287..5907
                     /locus_tag="ON430_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR3218346.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="ON430_000399"
     CDS             complement(5999..7165)
                     /locus_tag="ON430_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3218347.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="ON430_000400"
     CDS             complement(7177..7530)
                     /locus_tag="ON430_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR3218348.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="ON430_000401"
     CDS             7658..8680
                     /locus_tag="ON430_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCR3218349.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8699..8860
                     /locus_tag="ON430_000402"
     CDS             8699..8860
                     /locus_tag="ON430_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218350.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9119..9553)
                     /locus_tag="ON430_000403"
     CDS             complement(9119..9553)
                     /locus_tag="ON430_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218351.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="ON430_000404"
     CDS             complement(9663..11522)
                     /locus_tag="ON430_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3218352.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11640..12800)
                     /locus_tag="ON430_000405"
     CDS             complement(11640..12800)
                     /locus_tag="ON430_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCR3218353.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13107..14531)
                     /gene="araA"
                     /locus_tag="ON430_000406"
     CDS             complement(13107..14531)
                     /gene="araA"
                     /locus_tag="ON430_000406"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR3218354.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14547..15245)
                     /locus_tag="ON430_000407"
     CDS             complement(14547..15245)
                     /locus_tag="ON430_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR3218355.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15202..16863)
                     /locus_tag="ON430_000408"
     CDS             complement(15202..16863)
                     /locus_tag="ON430_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCR3218356.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17081..18481
                     /locus_tag="ON430_000409"
     CDS             17081..18481
                     /locus_tag="ON430_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR3218357.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18733..19812)
                     /locus_tag="ON430_000410"
     CDS             complement(18733..19812)
                     /locus_tag="ON430_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3218358.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19898..20857)
                     /locus_tag="ON430_000411"
     CDS             complement(19898..20857)
                     /locus_tag="ON430_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR3218359.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20869..22380)
                     /locus_tag="ON430_000412"
     CDS             complement(20869..22380)
                     /locus_tag="ON430_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR3218360.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22402..22965)
                     /locus_tag="ON430_000413"
     CDS             complement(22402..22965)
                     /locus_tag="ON430_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR3218361.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22971..23834)
                     /locus_tag="ON430_000414"
     CDS             complement(22971..23834)
                     /locus_tag="ON430_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3218362.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23848..24744)
                     /locus_tag="ON430_000415"
     CDS             complement(23848..24744)
                     /locus_tag="ON430_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3218363.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24919..26208)
                     /locus_tag="ON430_000416"
     CDS             complement(24919..26208)
                     /locus_tag="ON430_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3218364.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26180..27697)
                     /locus_tag="ON430_000417"
     CDS             complement(26180..27697)
                     /locus_tag="ON430_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR3218365.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27895..28785
                     /locus_tag="ON430_000418"
     CDS             27895..28785
                     /locus_tag="ON430_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR3218366.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28858..29682)
                     /gene="nadE"
                     /locus_tag="ON430_000419"
     CDS             complement(28858..29682)
                     /gene="nadE"
                     /locus_tag="ON430_000419"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR3218367.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29694..31166)
                     /locus_tag="ON430_000420"
     CDS             complement(29694..31166)
                     /locus_tag="ON430_000420"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR3218368.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31803..32165)
                     /locus_tag="ON430_000421"
     CDS             complement(31803..32165)
                     /locus_tag="ON430_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR3218369.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32388..33140
                     /locus_tag="ON430_000422"
     CDS             32388..33140
                     /locus_tag="ON430_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3218370.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33280..34203)
                     /locus_tag="ON430_000423"
     CDS             complement(33280..34203)
                     /locus_tag="ON430_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3218371.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34218..34490)
                     /locus_tag="ON430_000424"
     CDS             complement(34218..34490)
                     /locus_tag="ON430_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218372.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10228 a         7213 c         5978 g        11237 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc ctagatgagg caacataagt atacggtcat tttctaagaa ctcttcttct
    12061 ggaaaatcag tcaagtattt agccagatgt tgtttttcca atgcttgcat aacagcatat
    12121 ttatcaacaa tttcacttct tcctacattg ataagtacag catctttttt cattttttct
    12181 atattcgcct gatcgatcaa tccttttgta tcttctgtca acggcagcat gactacgatg
    12241 taatccgagg tagaaagaac tgtttctaaa tctgcttgcc gaacataatc caactgggca
    12301 tcgcggatag aataacccaa gacatccatc cctaagctat agcaacttag cgctactttt
    12361 gtgcctatcg ctccaagacc aagaagaccg atggttttgc cttgcaattc tcgtcccaca
    12421 taagcacttc ttttgttttc tgcctgttct aaaatattag gacctgttag tgtttgtacc
    12481 atacgacttg cttcgattat cggacgggaa gataagagca aacagcacaa aacaagctct
    12541 tttactgcat tagcatttac acctggtgta ttcatgacga tcgtcccgtt ttcagaagct
    12601 ttttcaacgt tgatcgtgtt caccccaaca ccagctcttg aaattgctag caaatctggt
    12661 gtaagccatt catctttcac tttcgtacta cgaataacta ttccttcggc tggcaaatca
    12721 tctattattt tgaaatattc cccttgtaac ttttctatct cttgttctgg aatagcatca
    12781 atcaaatgaa cgtgatacat aaaaccccct ccttactaac aagtatctcg aaatttctgc
    12841 aattagacaa ctgtttacca tttgacttcc tagaagcaaa aagagcagtt agagcagagt
    12901 aggctgagtg ataaaaataa ggctatgaca agttactgcc ataagcctta ttttcgagag
    12961 caaaatattg agggtgagat atcttgataa atggtgttcc gttagctgac attgataata
    13021 agactaaatt tcaaaaaagt tgaaaagcaa atttcgtcaa tctcttctta tttcttaaaa
    13081 ctaagtggct ttttcacaac ctctttttat ctaataacca ctgtttccaa ctcaactaat
    13141 tttgcccacg tttcaatttg atctgttgtt agatttagcg agacaacggt atgatgaccg
    13201 ccacctgcct gaatccatga atggacacct tcatggaaat tcggtttagt tttccataac
    13261 acacgtgcaa caggtaaatt aggtgcttca gtcgttggtt caaaggcttc tacttcattg
    13321 atcagcagtt tataatgcgt accgaaatct gccatcgata cgactacccc ttctcctgct
    13381 ttcccatcaa atacaagacg cgccggatct tctctatctc ccattgataa aggagaaaca
    13441 acgattcttg gtttcgtcgc agcaagcgtc ggatctactt ccatcatatg ggattccaag
    13501 attgcttctt ggccactagc tagctcgtaa gtataatctt ccatgaatcc tgtatctttc
    13561 cctcgagcca tgattttcag caaccgatca atcgctgcag ttttccagtc gccttcccca
    13621 gcaaaaccgt atccttctgc catcaaacgt tgtacagcta gtccaggtag ttgtttcatt
    13681 ccatacaggt cttcaaaatt agtcgtaaaa gcgttatagc ctccttcttc taagaaacgg
    13741 cgaataccga tttcctgctg agcttgtact tttacatgtt cctcccatgt cttttcttca
    13801 taatcaccat agtcgaaggt atacaattcc ttgtactctt caaataattg atcgacttct
    13861 tcgtctgata cgcgatcgat tacttgtaca agatctccga taccaaaata atcaacagtc
    13921 caaccaaatt gaatctgtgc ttccactttg tctccttctg tgacacccac gtttctcatg
    13981 ttgtcaccaa aacgagccac ttttattcca aaactttcat tataggctac tgcaacatcc
    14041 atccaatcag caatctcttt ttggatctca ggtcgtttcc agtagccaac gacgatttta
    14101 ttttgtttat ttaatcgagc attgatgaac ccatattcgc ggtcaccatg agcggattga
    14161 tttaaattca tgaaatccat atcgatcgtt ttccaaggaa tgctttcgtt atattgcgta
    14221 gctaaatgaa gcaatggttt ttgtagaagc tttgtcccgc ggatccacat ttttgctggt
    14281 gagaaagtat gcatccaagt gatcacacct gctacttctt ctcgataatt gacctctttc
    14341 atgatttttg tgatcgtatc agcagtaact gctaattctt gaaggcggat tggataaggc
    14401 aattggccat tttcattcag ttcatcgacc atttcttgtg catgtttctt tacttctcgc
    14461 agtgcttctt ccccatataa atgctgtgat cctacaacaa accaaaattc tttttctcca
    14521 atattcaaca taattagcca ctccttttat ttgttttgtc cgtaatacgc attttttccg
    14581 tgttttcgta aataatgttt atctaaaatc cgttgaggca gctcttctga aaatgaattg
    14641 agctgtctgg taaataaatt catcttgcat acttcatcta gtacgactgc gttcattacc
    14701 gcactttgcg catctttccc ccaagtaaat ggaccgtgtc cgtgtaaaag tacccctgga
    14761 attgctaagg gatcaatttt gcgttctttg aatgtttcta tgataacttt tcctgtttca
    14821 tattcgtacc cgctgtcgat ttcctgttgt gtcaaaaatc gagcacaagg taccgatcca
    14881 taaaaagtat ctgcatgcgt cgtccccatc gcaggaacat ccaatcctgc ttgtgcccag
    14941 atcgtcgccc atgtcgagtg agtatggacg attcctccga tttgaggaaa tgaacgatat
    15001 aaaacagcat gagtaggcgt atccgaagag ggattgagtt taccttcaat gatatgattg
    15061 tccaagtcca caacgaccat gtcgtctgct gtcatctctt cataactgac tccgctaggt
    15121 ttgataacga aatatcgtgt ttcaggatca aaagcgctca catttcccca tgtatatttg
    15181 acgagtccgt gtttaggcaa gtcaagattt gcttggtata cttcttcttt cagttgttcc
    15241 agcattttct atcactcctt tttcttgcaa ttgatgaatc gctgtttttt caatgtcgat
    15301 cccctcacgg taacgtctca taaatatctg atagcctgct atttcttctg ctgaaggtgt
    15361 gatggtgatt ccttggtctt gtccaaacac gtcttctgcc aagaagtttt ctaaagactt
    15421 cccatgtcga tccatcatat aggctgctaa taacgcgatt ccccacgctc caccttctcc
    15481 ggctgtgtcc atcactgtaa ctggtgcctc catagcagat gctagaatcc gctgtccaac
    15541 ttctggtgtt ttaaaaatcc ccccatgccc cactaaacga tcaatttgga cattctcaga
    15601 tttcaatata tccattccga ttctcattgc accaaaagca ctggctaaat ggatccgcat
    15661 gaaattcgct aaatcaaaac ggctacctgg tgtacgtaca aataatggcc ttccttcatt
    15721 tactcctgta atgttttcac cggaaaaata accataagaa agtaatcccc cgcaatccgt
    15781 atcgccttct agcgctttgt tgaataatac cgtgaataat ttatctctgc ttatctccat
    15841 acccaaagaa ttggtaaatt cttcaaaaat actgacccaa gcattgatat ccgaagagca
    15901 gttattcgta tgaaccatgc ctactagttc tcctgaaggt gtagtgacca tgtcgatttc
    15961 cggatggaca ttttctagtt ctttttctaa tacgatcata gcgaaggcgg aagtaccagc
    16021 cgaaatgttc cctgtccctt tggctacact gttcgtagcg accataccgg ttcctgcatc
    16081 cccttctggc ggacaaaccg ggattcctgg tctcaaattt cccgtagggt cgatcagata
    16141 agcccctgat tcgcttaact tgccagcttc ttctcctgct actagaactt taggcaaaat
    16201 gtgatccaag tcaatcgcta cgtgcgcttt ttcgattaac tgaccaaaaa tatttttcat
    16261 ttcttgatgg taggttttcg ttgaaggatc gataggaaac atgcctgatg catctcctac
    16321 gcctagtact ttctcaccag taagctgcca atggacataa cctgcaagcg ttgtcaaaaa
    16381 attaatttct ttcacatgtt cttctttatt caaaatagat tgatacaaat gagcgatact
    16441 ccatctctgc ggaatattgt attgaaattt ttccgttaat attcgttctg cttcttcagt
    16501 gattgtattt cgccaagtac gaaatggtac tagtaactgt tccttgctat caaatgctag
    16561 ataaccgtgc atcattgcac tgaacccaat cgacccgatt gtcgtaagat tctcgccata
    16621 gatttcgaat acttctgctg ccatttttcg gtaactgacg cgcagtcctt tccaaatatc
    16681 ttctaatgaa taggtccaga taccattttc tagtttgttt tcccactcat aactaccagc
    16741 ggcaatagga gtaaaagatg aatcaatcaa tacagctttg atcctagttg atccaaactc
    16801 aatccctaaa gatgtacgac catctatgac atctaacata ctttgcgcat ttacttcctt
    16861 cataatgatt taactccctc cttctcgtca aacctgatca aaccgtcttt ttagtagcgc
    16921 ttacaatgat tattgtatac ttttgtacgt acatgtcaag cagttattta caagaataca
    16981 ataaaaaaca cttgttttgt acgtacataa gttataaaat gaaattgctt acaaaatctg
    17041 atcaaccgcc ttctcgaaat tctagaagga gagaatactt atgaaaaaag aaaaaaaagt
    17101 ttcctcatcg tttatttatt tttttggcgc atttggcggc attctatttg gatatgatat
    17161 tggtgttatg accggcgcct tacctttttt acagcatgat tggggtttgg caggtaaagc
    17221 ttctttgata ggatggatca cgtcttcggt catgttaggt gcgatccttg gcggctcttt
    17281 atctggtctt ctttctgata agcttggacg acgaaaaatg attcttcttt ctgcacttat
    17341 ttttatggct ggttctgtcc tttctgcatc tgcccctcac aatggttctt attttctgat
    17401 tgcagcaagg attcttctag gtttagctgt tggcgctgca tctgccctag ttcctgctta
    17461 tatgtctgaa atggctcctg ctagactaag gggaagattg tcagggatca atcaagtgat
    17521 gattgcttct ggcatgctcc tttcctatgt tgcagattat ttgctaaaag ggttgcctga
    17581 aacgatggct tggcgcgtga tgttagggct cgctgctgtc cccgcgctga ttttattttt
    17641 cggtgtttta gctctaccag agtcaccgcg ttttcttatg caatctggcc gcttggaaga
    17701 ggcaaaaaga gtattgaact atattcggac accaaaagaa gctgaacagg aattcgaaca
    17761 aatccagctg aatgtcaaac aagaaaagac aaccggaact tcttggcata ctttgttttt
    17821 agaaaaatat cgttccttag tcattgccgg tattggtgta gcagtattcc agcaatttca
    17881 aggagctaat gctatttttt actatattcc gctgatcgta gaaaaagcga ctgggcatgc
    17941 agcgagcgat gcattgatgt ggccgatcat ccaaggaatc attttagttg ctggttcttt
    18001 gctctttttg gttattgcag acaagttcaa tcggcgaact ttattgaaaa taggcggtag
    18061 cgtgatggga ctttctttca tcctaccagc tgttttagga actgtactgg atgctcatac
    18121 aaattcgttg ttgattctct tgtttttatg tatttatgtc gcattttatt cttgcacatg
    18181 ggctccactg acatgggtaa tcgtaggcga aatattccca ctagctgtcc gcgggcgagc
    18241 atctggccta gcctcttctt ttaattggat tggttctttc ctagttggct tgctttttcc
    18301 tgtgatgact gcttctatgt ctcaagaaat cgttttcggc atttttggga tcatctgctt
    18361 tttaggggtt ttatttattc aagagatcgt accagaaact cgtgggaaat ctttagagga
    18421 aatcgaacaa agtgcatcta aaaaaactta tcctaaacgt atttctatcc atcattctta
    18481 aagaggttgt gacagaagtg gtcagcttca agaaataaga aggaaccgct tagtttccag
    18541 tcacaaggaa caatttcaaa aaacagcttc tcatgtttga tgaatattgt gacttgtgac
    18601 cagttggtag gagaacaccg tttagtggag aggtggtgaa agaaacgttc agccttgagc
    18661 gatgacaaaa gctctcacaa acattgcgac gagtaaccgt aggagcaata agaggcggta
    18721 aaaacccgtc tcttattttt cgttttcttt cactgtttcc cgttcaacta gtacaggttc
    18781 atagagaata tttggctgtg cctgtccttt ttcaatcgtt gtgacgatcc accgtgcggc
    18841 atcttgtccc aacttttctt ttggatgcgt caatgttgtc aattttttat tccctaaatg
    18901 gcttaaagaa gaatcgtctg ttcctacgat cgataggtca tctggaatat gatagccatt
    18961 ttctgtcaac ttatctagca ttaaactagc tatttgatcg ttatagcaaa tgatgccatc
    19021 taaagcttgt tcttctttca accgttgtac tgttttttca gcgattgttt ctcgtgtttc
    19081 cgtggtgaaa gtaatgatat cttccgtcaa aaaacgcaca ttatactgtt cacacgcgct
    19141 gataaaacct ttcatcctgt attttccttg aagatcgtcg atttttgtga tgaacaacag
    19201 acgctgatgt ccttttttta tcaaatattc tgctgccata aaaccgactt tcacatcatc
    19261 taaacaaata taagaggcat ttaactcttc ataagcagca ttgatcatca ccatcggaat
    19321 gccttgttct ctcaaagcaa cataaagtgc taagtttggg ttatactgat tacttttcgt
    19381 aggctcaacg atcaaaccat ctaccccata agcaatcatt ttcttcaaac actctttctc
    19441 caattcgtga tcgttattcg tactggctaa aagcaatgaa tacccagatt ctcgtaattc
    19501 attttcgatt cctcggataa tcgacggaaa aatataatca gagagatatg tggtgatgac
    19561 accgatcgtt tttgttctgt tcaatggtgt tttttctttc ttatagctgt catctacata
    19621 tgtgcccgac cctttttctt tacgcagata gccctcattt accaacacag cgattgcttg
    19681 ccgaatcgta tgtctgctga cgtcatatat ttcttgcagc ttgaattctg agggaatggt
    19741 tgtatttact tcgtattcct cacgaaggat cttttgtttt atctcttctg caatatcttg
    19801 atattttgcc atttgccttg ccccttttta tttgtacgta catatatcat aacgagtctg
    19861 caaggtttat tcaagcaaaa atcaaggttt cttgtgctca taaggcatac ggaacaggtt
    19921 caccaaattc tggaaatcct tgctcattcc acgtaaagca ttgcgcgttc gcgtgccgat
    19981 taggattgtc taacggatcc cctttttggt tcttctctgg tcgagcatga tagatcaaaa
    20041 tatcaaagca cccgtcttct gaaaccgtaa acgagttgtg accaggacca aattgcttat
    20101 ttttttcaga agaaacgaat actggctccg ataatttatg ccatgattct tttaataaaa
    20161 gattcttgtg ctcatcagcc cacagcaggc ccatggcata gttttcatct gttgcactac
    20221 cagaatacgt aatgaatatt ttcccattgc gagtaaggac tgctggacct tcatttactg
    20281 aaaaaccccg tttttcccat tcatactcag gtatagaaag gaggatctgc ttccccttta
    20341 acgtccaagg attatccatt tcggatagat aaagattaga attcccagta atttcaggat
    20401 cttgttgggc ccacacataa tatagctttc cttgagaaga gaaagttgtt ccgtctaaac
    20461 tgaaagtttc ccttttcgtt tttatttgtc ccttttctat ccaatctgta ttcattggat
    20521 ctatatgctg attttcaagt acgaacatgc ggtgatgatg agattcatca cgtatagttt
    20581 tatgattgct tgcagcaaag taaatatacc attttccatt gatataatgg acttcaggtg
    20641 cccagatcaa ttcactcatc ggtccttctt catgagcctg ccatacaatc aggcttgcag
    20701 catgttctaa ttcattcagt gactttgccc ttcttaattc aatcacttgg tagcctggaa
    20761 ccgatcctgt aaaataataa taaccatctg tatgtttata tacccaagga tctgcccgtt
    20821 gccgaacgat aggattatta taaagtgtct tcatcataac aaatttcctc acattttctt
    20881 tattctaatt acattccaag agtatttttc tatccctaca tttaattcgt catgtttttc
    20941 tattgaaaaa tagtttattt caggttttat tttttctggt tcttcacgtg tatttttgtc
    21001 caacagatcg ctactggtta atgtgtagtg ctctccttga tctagcaaat tgaaatcatg
    21061 ttcaatcgtt atcttttgtt gttcatttgt gcgattgatc aagaaaatag tcagctgatt
    21121 gccgttatct acgatcacac tgtccacata aggaacattt tttatctttt cacaatcata
    21181 aacagggcaa atgatttgtt gtttcaatac ttgaccttta gcatatttcg ataagtgttg
    21241 catcacataa taagttggat tcctccatgc ttgtccttta gcatctgtca gtattaaagg
    21301 tattgtgttt actaacaatg attgacaact aatctttacg cgatctgcgt gtcgtaaaat
    21361 agccagccct aatgaaccaa ataataatgt atcttccata gtaaagtaac accaatcgat
    21421 gggactaccg acttcaaaat ccctatgtct tttttccggt tgggcatgta cattccattc
    21481 atcaaacgct agatacattg ttttctcact tctcaaatat ccttttacat aatcacaagt
    21541 agcaacgatt tcttcaattt gacgatcgaa tcgataagct tttgcaaggt atgtcgctgt
    21601 atcatcacgc tcatttttag gaggtttagt caagtcttct tcatcatact tatcaatata
    21661 gttatgcaaa gctaaataat ctacattctc ataagcttct tctaatgcga tacgatccca
    21721 ttcagggtac gatggtaaac gtggcgtaga gcttccaact aagattgttt caattgaatc
    21781 atccactaac ttcatgactt ttgaggcttc atttgctaac ctaccatatt cataagcact
    21841 cttagccccc acttgccatg gaccgtctag ctcattccct aaacaccata atttaacgcc
    21901 aaaaggcttt ttttgcccat tttttattct taaatcactc cagaaggttc cttttggaaa
    21961 attacaatat tctactaaat tttttgcttc atcaattcct tttgttccta aattcaccgt
    22021 gaatatcggt ttcgcttgta catgactgat ccatttaaaa aattcgtgga gtccaaaaga
    22081 atttggttct atacctcgcc atgctaaatc aatttttttt ggtcgttgtt ctttcctacc
    22141 tacagtatcc acccaatcat aaccagatgt aaagtttcct ccaggatagc gaatgacacc
    22201 taattctaat tcctttacat aagagaggac atcttgtcta aacccgtcac catcagctgt
    22261 ttcatggccc ggttcataaa tccctgtata gacgacactt cccatatgct caacaaatga
    22321 gccaaacagc cgagaactga tctctcctac cgtaaaatcc tctctgattt ttactttcac
    22381 ttcttcgtcc aacagctttc cctacctttc atttcctata gtcaagataa tagatacaag
    22441 tttccatacg ccaccagcaa agataaaaat aaaaggaggg aaaagaaata tgagcagtac
    22501 aaatcctaat aacaatacgc aacaaagaat atttctcaaa aaatcaaaaa atactttcca
    22561 tgcactttgt tcaaaaattt gtaaaaatgt gatatctttg ttttcagaaa tcacatatat
    22621 ccaggataaa agaaacatgg aataaataag taaaactaca tatccactaa ttgaaagtac
    22681 gattgtctgt tcttctaata attgattaac aagaaaataa ataatgatcg ttaacagact
    22741 aaggaacaag ttcttaccaa aatgttgctt tattccttga aagaaaaaat aaatgatatt
    22801 tcctgatccc tcacctttcc attgcgccat tacataaaaa acagcacatg tagcaggcac
    22861 gatagtaata acaggcaaac acacgattac ccagagaata cctaataaga tgtaatcaga
    22921 aaccacaatc aactgtccat aaagtttagt ctcaactatt ttcatcttcc tcactctttc
    22981 attccagaca tagctgttcc ttgaacaaaa tatttctgtg caaaaatata aaccactagt
    23041 aatggcagca ttgaaataac cgttccagcc attacaggtc cataaaaaga aatgttctgc
    23101 cctgtaaata aagctaaccc tgctggtaaa gttctcattt ttgaactact tgtcaatatc
    23161 aatggataaa gcaagtcatt ccaactattc atcagcgtga aaatccctaa tgataaaaga
    23221 gtaggtttgc tcaatggcaa catgattttc aaaaaaatat taaactcact taatccatca
    23281 atgcgtgctg cctcttccaa agaatctggt aaagtaataa aaaaggaacg catcatgaag
    23341 ataccaaatg cagttgtcat acgaggaatg atcaaacctg cataagtatc aagcatgcca
    23401 agcaaattga cttcaataaa tagaggaatc atgaagactt ggaatgggat catcattgtc
    23461 agcaaaacga aatagaacaa aactgatttt cctttgaaac gcatacgagc aaatgcatac
    23521 cccgcaagag aatcaaagaa tacagaagtg atcactgtcc ctccagcaaa aatcactgta
    23581 tttttgatgt aatctaataa agggatcgtt gtccaaacct taatatagtt atctagggta
    23641 tagctatctg caaatattct aggcggaaat gagataatgt ctttttcgtg tttaaaagaa
    23701 gaaataatca gccacaaaaa aggaaacgca ataaccaaca atataactag catactaatc
    23761 gttcgaatga tttttttacc actaactgct tttcgactat tcacagtaac tgtaatcgag
    23821 ctttctggtg ccatacaggt ttccctccta tttcatttgt ttttctttcc gtgacatgta
    23881 cacattagtg gagatagcta tagttgcaac aatgataaac aaaaccgaag acagtgctga
    23941 tgcatagccc aattcataag gtgctgtaaa tcctcgactg taaatatatt gaacagctgt
    24001 ttctgtctta aattgtgggc caccagcggt tgcaacatat acttggtcaa acacttgcat
    24061 cgaaccaatc agattcgtta aaatacaaaa acctaaggag ggaacgatct gaggtagcgt
    24121 aatgttaaaa aactgctgtc gatttgttgc accatccatt tgagcagatt catacaaaga
    24181 gggagagatt ccctgaatag cagtcaagag aatcgtcata gtaacaccaa aattcttcca
    24241 aacagtcatg atggctaccg tcggcatagc aagggttgga tcgcgaaaaa attgagggat
    24301 cggcaagccg acttgtgaaa acaagtaagg aatcgcccct acattcggat caagcaacat
    24361 agaaaaaata atcgcaattg acgtcaatga acaaataact ggaatgtaaa atgtcgaacg
    24421 acaaaaacga ttgaatctgg tatttttaga taatgctact gctgctaata aaccaacgat
    24481 aatttgtgtt ggcgtttcga gtacggtgaa atataaagaa tgaatcaacg aattaactgc
    24541 tcgttcatca ctaaatatac gtgtgaaatt ttcaaaactt acaaaatttc ttgatgtaaa
    24601 gaaaatattc aaatctgtaa aactaatagc aaatgttcct aaaagcggta tgacagtaaa
    24661 taataataaa actaaaaaag atggagttaa aaacaaatac gcagcacgat caggacgatt
    24721 ccaatatttg gtgaatccgt tcatatagtc cacctcatct ttcattttga taaaatatac
    24781 aatcttggtt taaacgaatc tgaataagat atccgaacaa tcgctgtgat ttttgcaata
    24841 acacctatct gcaaatacta ggtcgatcat tcagatatct gtatcttgcc aacagtctat
    24901 cctgtataaa gttctgtact atttttgcag taattgattg atggcatcat ctgcatcgtt
    24961 catgagtgtc tcaggatctt ctcctgctaa taatttttca atcagtggat tgatgatatc
    25021 gttattgatc gcagagattt gggtaaaacc tggtaaatat ggctctgcat agtccatttg
    25081 tttcgataat tcagatacaa taggatcatt ttttacttct tcatcttttg ctactgaatc
    25141 caagtaggca ggaaaaccat tttcgatact ccatttttta ccaatttcag ttgtattcca
    25201 atatttcaaa aactcataga cagcttcttt tttattatta tctgtacttg ccggaatggc
    25261 atatcctacg ccatctaaaa tagctgtttt tttgtctgaa tcactccctt gtggtaaggt
    25321 agtcacatta taatcaatct cgtttgcttt caatccgtta tttaaccatg gcccattgat
    25381 ttcaatggct agttgaccag cattcattaa attgtccatt tcggcgccag aaatcgattc
    25441 tggaccagtc ttgtctacat ggatcatttt ttggatcgct tttaacgttt taaggttctc
    25501 aggagacgca aattcagact tagtataatc atcgttcact aatttaccgc catttgctaa
    25561 catccagtta tataacggtg cagttccatc cttattgaag acaaaacctg atacattttt
    25621 actactatca gttaattttg gtgccatttc tgtcaattct tcccaagtag taggtggttg
    25681 ttcagtatct aatccagctt gtttaaacaa atcattattc caatacatat acatgccttg
    25741 tacctgcatg gggacaaaat actgtttacc ttctacttca cctaattcaa tagccgtttc
    25801 tttaaaatca gatttatcta ctccatcata ttcccagaaa tcgtctaatg gttgtaccgc
    25861 tccgttttct acatactgtg caaaatctgg ataatttaat gcaataaagt caggtgcttt
    25921 tttagaagaa atcgctgtag gaagtttttc attcaaattg gcccaagtca tgatatccat
    25981 ttcaattttg atgtttttat catttgtttt attaaattca ttaacgattt cttgcaatat
    26041 ttcaccatca gatgcagtaa acccattcca aaaagtcaaa gtcacttctt tatcagaatc
    26101 agattctgct gaacttccgc caccgcaagc tcctaaaata cctgttgcag ctaccaacaa
    26161 agagccaatt aaccattttt tacctttcat tttttgttcc tccttcatct gataaacgac
    26221 atctgaccac attccaagat agcggtttca ataaacaatt gattttccca tcttcagctt
    26281 ttacatttgt tgaatgactt ggttttactt gttcactttt aggaagatta gttgcttttg
    26341 tatcaaatcc actcatttca gaaaaatcaa tcactccctc taatgataat tcagacaagt
    26401 gaacagttag attcatttct tcttctccac ggttaactgc aaatatgaca agttctttgt
    26461 catttattac ggctatactt tctaaatacg gcacttcttc aaaatcttcc gtcgcatagt
    26521 ttgctgaacg aatttgtggg cttaacacct ctccttgtcc atacacagca gcttgcataa
    26581 atggataata gatcgtctgt ctccaagctt ctccgtttgt ttctgtcctg attggtgcaa
    26641 taacatttac caattgagca agacaagcaa tctttactct atctgcatgt ttcaataaag
    26701 tgattaatag acagcctaat agtaatgcat cttcaaaatt atagttatct tctagtaaag
    26761 gaggagcaat ctgccaaggc gctgcttgct catcttgttc cgctgaatga taccaaatat
    26821 tccattcatc aaaggacagg ttgatctgct tcttactatg tttcttcgcc ttgactgtat
    26881 cgcacatcgc tgtcacttca gaaatgaacc gatccatgtc taaggaacgt gcaagataat
    26941 gagaaagatc atttttttgg ttgccataat attgatgcag agacaaataa tctatttgat
    27001 cgtacgcttg atcaagaact gttaactccc aatcaccaaa tgtaggcatt tgccgattcg
    27061 aacttccgca taaaaccacc tcaattgaat catctacgag tttcatcgcc ttagccgttt
    27121 cctctgctaa ccgtccatat tcttccgctg ttttatgtcc aatctgccaa ggaccgtcca
    27181 tttcattccc taaacaccat gtcttaattc caaaagggct ctcttgacca ttcgaacgcc
    27241 ttaaatcact ccaataagtt cctttaggaa aattacaata ttcgactaaa ttacgagctg
    27301 catctactcc acgagtaccc agattgacag ccatattgat ttcggccccc acatgctgac
    27361 accagttata aaattcatga atcccaactt cattcgtttc aattgtccgc caagctaaat
    27421 ccaaccttct aggtctattt gctatgggcc caatactgtc ttcccaacga aatcctgaca
    27481 caaaatttcc accaggatat ctaatcatgg gaatgttcaa ttcttttacc aagtgaatga
    27541 cgtcttggcg aaatccactt ttattagctg atggatgatc tggctgataa atcccttcat
    27601 atactgctcg tcccatatgt tcaataaatg aactgtacaa ccgcggatca actttgccta
    27661 cacgcttgac actactaatc aacattgtgg cttccataac tgacctcctt taatttatac
    27721 atgaataata cacctcgttg aaagcgttgt caataatata aatgttaaac ttacttattt
    27781 tttatcgatt ttaattaacc aaaatattaa taatgattaa aacaaataat ttaatataac
    27841 ttttattaac aaatatgtta atatataaga aaaacctttt aagaggtgat atatatgcaa
    27901 ctacaaataa acgaagaatc cttaccggta tatgaagcat tagctagtaa aacaaggatc
    27961 aagatcatcc agctactttc caaaaaaaag atgaatgtca aagatttggc aaaagaactt
    28021 ggagtgagca gtgctatcac gacgatgcac gtgaaaaagc tagaggaagc aaacatcatc
    28081 aaaacagaaa aagtaggaca acaaaaaatt tcgagccttc gtgtcgataa aatcgatatc
    28141 agttttccag aaaaaatatt caatgctttt gatacgaaag aaacatcgat acctattggt
    28201 cactatacaa attatgcaat tgaaccaaca tgcggtttag ctacgatcca tgattttatc
    28261 gggaaagtgg atgaaccacg ctattttatg gatcctcgaa ggatggatgc ccgcattctc
    28321 tggtttacca gcggatttgt cgagtatcag gcacctaatt ttttaaatac agaggataca
    28381 ttggagatgt tggaagtttc tgtggaaatt tcttctgaat ttcctttttc aaatgacaat
    28441 tggccttcag atatcacttt cagcctaaat ggtgtggagc taggaacatg gacgagccct
    28501 ggagattttg ctgatatccg cggtaaatat acgcctgatt ggtatccaga taatcttaat
    28561 caatacggct tgctgaagac gatccgaatt accaaacact tgacgaatat gaatggagaa
    28621 cccttgtcaa acatcacgat caacgacatc cctaaagagc aagacacttg gcatttgcga
    28681 atcgaagtga aagacgatgc caaacatgtg gggggctgta ctttatttgg taaaggattc
    28741 ggtaattacg atcaggatat caaattaaaa gtttactaca gctgatattt ttcttttcta
    28801 ttttataaaa caaaaacagc caatccctat gaagggacgg ctgtttttgc tttattgtta
    28861 tttccagaaa tcatccaaaa cggaaattgg caaatgtcgt ttatgttgtg tcttgttcca
    28921 ccatgcttcg attttttctt gtgcttgagc gggaacttct tttccttcta gataatcatc
    28981 aatttcatta tatgtgacac ctagtgcgac ttcatctgca atcattggtt tcccatcttc
    29041 aagatctgca gtcggaattt ttgtgtataa tttttccggt gcattcaatg cttgaagcaa
    29101 ttgtttccct tgtcgtttgt ccaaacggaa taatggcaaa atgtcggctc caccatcacc
    29161 aaatttcgtg aagaatccag taatgttttc tgcagcatga tctgtcccta atacggctcc
    29221 tgctctttct ccagctacgg catattgcgt aatcatacgt tgacgtgctt tgatatttcc
    29281 tttgttaaaa tcgctgattc ccacacctgc ttcgcttaat acttgtactt gtgcatcaac
    29341 agctggtttg atatttactc gcagactaac atcgggctgg ataaagtcaa gagcagcttt
    29401 tgcatcttct tcatctgctt gttcaccata aggaagacga acagcgataa attgatagtt
    29461 ttcatctttt gtttcttcac gcatttcttc catcgttagt tgcgccaaac ggcctgctaa
    29521 agtagaatct tggccgccgc tgatacctag tacaaaagtt ttaagaaacg gatatttttt
    29581 caaatatgct ttcataaagt caatactttt acgtatttct tcttttggat caattgttgg
    29641 tttgacacct aattcattga taatttctgt ctgcaggctc atttattctc ctcctatgct
    29701 tctgtatgaa gttctgctac gctttttttc attcgttcca tgatagccat tttatggttc
    29761 cagcattcag tagacagatc gactgggtat ttttgcggat tcaagtctcg tttatactct
    29821 tcccatagtg aatctagatt ttcttttgta tattctttga tttcgtttag tgcaggtaat
    29881 tcgtaaactc tttttccttc gataaagata tcttgtaaaa ctgggcgagc tgtaaagtct
    29941 cgaacagttt tattgataaa cgtatgcact ggatggaaca tgaagatagc ttcttcttct
    30001 cgagggtctt catcccataa tgtcacgtag tctccttcag atttcccatc aaaatttcga
    30061 gtgatgcgcc acacttgttt tttccctgga gtagttactt tttctgcatt gcttgataac
    30121 ttgatcgtat ccatcatttt tccttcatca tcttcgatcg atactagctt gaatactgca
    30181 ccaagggctg gctgatcata ggctgtgatc aattttgtcc caacacccca tacatcaatt
    30241 ttagcttttt gcatctttaa attcaaaatc gttgcttcat ccaagtcatt cgaagcatag
    30301 atttttgctt ccgtaaatcc ggctgcatct aattgttccc gaacacgctt agatatatag
    30361 gccatatctc cactgtcgat gcgtacgcct tggaagttga ttttgtcacc taattctttc
    30421 gccacgcgaa tcgcacttgg cacacctgat ttcaacgtat cataagtgtc gactaagaac
    30481 acgcagtctt tatgggtttt agcgtaggcc ataaatgctt cataatcatt tccataagat
    30541 tggactaacg aatgagcatg ggtcccgcta gctggtatcc caaatatctt cccagcacgt
    30601 acattgcttg ttgcatctgc tccgccgata tatgccgctc tagttcccca gactgctgca
    30661 tccagttctt gtgcccgtct agttccaaat tccaatagtg gatcttctcc gatgacagat
    30721 ttgatacgag ctgcttttgt cgcgatcaac gtttggaaat tgaccatatt caacagtgct
    30781 gtttctacta attggcagtg cgctaaaggc ccttccactt ggattaatgg ttcatttgca
    30841 aaaactagtt ctccttcacg agcagaacga actgttgctt taaattcaaa attttgtaaa
    30901 tactccaaaa aaccttcagg atagacttct aatccacgca agtaagcaat atcggaatca
    30961 ttgaacgtca gattctctaa ataattgact aaccgttcca atcctgcaaa aaccgcgtat
    31021 ccgtggttaa aaggcatttc tcgaaagtaa cattcaaaaa cagcatgtaa atcagctcga
    31081 cctaattccc agtatgtctg catcatattg atttgatata gatccgtatg cagcgtcaaa
    31141 ctgtcgtctg ggtaagtatt attcatcttg tattactcct cttggcaagt tttcatttcc
    31201 tacccattat aacaaaaaaa attaatagga aaagaaatct tccatttttt ctgaaaaagc
    31261 acatgtttta cggatatttc ttgctaaaaa tgaactccat ctgctaaaac tatcctttct
    31321 tattggtatt taacctaaac gagaaagaat acttatttta ttcaatgtat ccgagacacg
    31381 aagaaattag cgttttctat ttttcttact tgataaaata agtagatttt cctactacaa
    31441 tcgtagccgt taagataccg atgattgaac aaatataacc aaatgtctct acaacataat
    31501 caatggcaaa agtttttgaa gcaataatac gtctttcctt ttttgcttct tatccccaaa
    31561 gaaacggata taacttatta tgtattgaat ttttttggaa aggcgaatta tgacaagata
    31621 taccttgcgt tgaaaaaact cgcggaaaaa atatcacacg tttttaaaaa gatacaaagt
    31681 aagaaagcag ctgtcaatag aatggaaaca aaaatggcga gacatcaatc agtctcgcct
    31741 tgttttttat tgtatacatt agatatggag caggttacca taaagatgat tattagtcgt
    31801 tattaaatag aatccttcgt atttttttgt tctatcacta caatatcttt atttggacgt
    31861 ttgaattgta ttccggcacg ttctagctcc tgtttcactc ctacatggac tttgacatct
    31921 tctcgtgtca tttttaaaac gtgatgaatc gcatagtcga tttcttgcct tgtgattttt
    31981 ttcttcgtat tgatcatcac gacttgcttt ttatcatatg tgtctaccaa tagcagttgt
    32041 gttcgatcta acgaaaataa tttgtaataa ttaatagccg tattcccaaa aaagaattcc
    32101 actactttag gataaaatgt cttaatattt aaattactat tcacaggtgt ttcgattagc
    32161 ttcatctcat tcctcttttc tatatagtcg atttagatcc aatcttaagt tatgttacca
    32221 tctttcctag cattaaggaa tcggttacaa ttgaaccttt aaagacaaaa ggatttacct
    32281 tttatcactt aatagtataa cataatttcg tgagaaaaaa atacaatttt ttcttgacca
    32341 tctcgttacg taaaggatta tgcttacatt tgtcaggagg aaaaataatg gagtacacaa
    32401 taaagaaaat gtcagagata tcaggagtca gtccgagaac cttgcgcttt tatgatgaga
    32461 ttggactgct aaaaccagct aggatcaatt cttcaggtta tcggatttat ggcaaaaaag
    32521 aagtcgatcg tctgcaacac atcttatttt atcgaacaat ggcattcaag ttggatgata
    32581 tccaagaggt cttagacaat ccatcttttg accaccagaa agctttaatc aagcatcagc
    32641 aaatgttgct ggaaaaacgg gcacaaatcg ataccctctt gacaactgtc caacagacac
    32701 ttgatatgta cgaaggagga agaaaaatga gtgacaaaga aaaatttgaa gggtttaaac
    32761 aacaaaagtt gaaagaaaat gaagaatcct atggacaaga gattcgacaa aaatacggaa
    32821 aaaaaaaggt tacagcatcc aatgaaaaat ggatgaatat ggataaagaa acgtatgaag
    32881 aaatgcaaac tgttgaaaag cagatgttat ttgatctcac tctttactta aaagaaccag
    32941 aagatgaaag cttagcagac cgtatcttcc aagctcataa gaagtggttc tcttacagct
    33001 ggcctgaata tcagccaaaa gcgcataaag gactaggaat gatgtacgaa gccgatgaac
    33061 ggttcactgc atattatgac gagcgaagtg gtcatggtgc agcaaaagct ttaaatgaaa
    33121 ttatccaaaa aaaggcgtaa tcttttacat gatccaacaa aaaagtacac gtaaaatgac
    33181 ctaataacaa aacagcagac acccaagtta gatttttatg ctaacttttg gtgtctgctc
    33241 cattctttca caagtttttt catcctactt taaatatatt catcgttgaa taaacgtata
    33301 aggtattttc actttatcct ttttttctgt aagaaacaat tgaaatgcca tttctccgca
    33361 agcttccaga tggtggtcga tggttgggaa accaagaagc tcgctgatca gcaaattatc
    33421 gcgtccaatc accactggtg tcttatcttg cagatagctt tgcaagatgc ctgctgcaac
    33481 catatccgaa tttgtaaaga caccatcaac tccaagctct ttaaaaaaga agcctgcttg
    33541 ttttcctttc tctgcatcta tacaatccca aaaaacaagt gattcatcaa aagatggaaa
    33601 aagttgatga catagctgca aagtgatttt tgagttataa cttagcctgc cacttcgccc
    33661 caacgtgatg cccaattgct ttataccttg ctgcttcaaa taagtaagac cttctgtaat
    33721 tgattgttct cgatcaataa agacacagct tgcctgtttt cccttgattt cttcacaaaa
    33781 aactagaggt ccatattttt gatatgccaa taaatcatct agtgtattgg cacgagacgt
    33841 aatgatcaat ccatcgaaag ctttagctgc aaattcttca agataccttc gctctttttc
    33901 tttatgatag cccgtcggta aaagtgtcac tttgtaatca taatcaaacg ctgcttcaat
    33961 gatcccactg atcaactgat caaaatacgg atgattggta taaggcagaa tcactccaat
    34021 gcttttcgtt tttccataac ttaactgtct ggcatttgca ttagggatat aatcgacttc
    34081 tttcatgacc gcaagtattt ctttcctttt ttcctcatct acatatggat gtccattgat
    34141 tacacgtgaa atagtagaaa ctgaataacc tgtgagcttt gcgatatctc gaatgtttgc
    34201 cactgctgct tcgccaccta tacaaaaaga ttttcttgta aatagtctac cacagctgga
    34261 ccgcattcaa aaaactgata catttttcct tcatgcgaaa aactgatccg ctgcccgccc
    34321 caatatatat cttttgtcaa attttcgatt tcattcattg gtatttcttt tactatattc
    34381 ctattcaacc cttgaaaact gtacaacgtt aatacggatc ccttgaactc gacaaacgca
    34441 taaaaataat tgttcgttgt gccatctgtt atctctacat aagtattcat tgcaagccct
    34501 ccttttgata ctttgagtat agtgtatgaa acgcgtctca tagcaaggag tttgttttgc
    34561 caaagaaaag ttttattcct ctttcattat taccaaagtc tcttatttta aattgaatac
    34621 attataatga tactgtaaat atatctttat tattag
//
LOCUS       DAJSMI010000010        32910 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJSMI010000010 DAJSMI010000000
VERSION     DAJSMI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.010"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(100..678)
                     /locus_tag="ON430_000425"
     CDS             complement(100..678)
                     /locus_tag="ON430_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3218373.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(698..1366)
                     /locus_tag="ON430_000426"
     CDS             complement(698..1366)
                     /locus_tag="ON430_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218374.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1381..2451)
                     /locus_tag="ON430_000427"
     CDS             complement(1381..2451)
                     /locus_tag="ON430_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218375.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2622..3986)
                     /locus_tag="ON430_000428"
     CDS             complement(2622..3986)
                     /locus_tag="ON430_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3218376.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(3983..5542)
                     /locus_tag="ON430_000429"
     CDS             complement(3983..5542)
                     /locus_tag="ON430_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3218377.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5598..8078)
                     /locus_tag="ON430_000430"
     CDS             complement(5598..8078)
                     /locus_tag="ON430_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218378.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(8062..8397)
                     /locus_tag="ON430_000431"
                     /pseudo
     CDS             complement(8062..8397)
                     /locus_tag="ON430_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8411..9445)
                     /locus_tag="ON430_000432"
     CDS             complement(8411..9445)
                     /locus_tag="ON430_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR3218379.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9535..10215)
                     /locus_tag="ON430_000433"
     CDS             complement(9535..10215)
                     /locus_tag="ON430_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3218380.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(10499..11602)
                     /locus_tag="ON430_000434"
     CDS             complement(10499..11602)
                     /locus_tag="ON430_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR3218381.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(12231..12701)
                     /locus_tag="ON430_000435"
     CDS             complement(12231..12701)
                     /locus_tag="ON430_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCR3218382.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            13031..14671
                     /locus_tag="ON430_000436"
     CDS             13031..14671
                     /locus_tag="ON430_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCR3218383.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            14825..16147
                     /locus_tag="ON430_000437"
     CDS             14825..16147
                     /locus_tag="ON430_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCR3218384.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(16215..16649)
                     /locus_tag="ON430_000438"
     CDS             complement(16215..16649)
                     /locus_tag="ON430_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3218385.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(16714..17550)
                     /locus_tag="ON430_000439"
     CDS             complement(16714..17550)
                     /locus_tag="ON430_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR3218386.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(18292..18918)
                     /locus_tag="ON430_000440"
     CDS             complement(18292..18918)
                     /locus_tag="ON430_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218387.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(19213..19548)
                     /locus_tag="ON430_000441"
     CDS             complement(19213..19548)
                     /locus_tag="ON430_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR3218388.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(19535..19819)
                     /locus_tag="ON430_000442"
     CDS             complement(19535..19819)
                     /locus_tag="ON430_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR3218389.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(19875..21398)
                     /locus_tag="ON430_000443"
     CDS             complement(19875..21398)
                     /locus_tag="ON430_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR3218390.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(21391..22566)
                     /locus_tag="ON430_000444"
     CDS             complement(21391..22566)
                     /locus_tag="ON430_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR3218391.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(22570..22755)
                     /locus_tag="ON430_000445"
     CDS             complement(22570..22755)
                     /locus_tag="ON430_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218392.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(22721..24415)
                     /locus_tag="ON430_000446"
     CDS             complement(22721..24415)
                     /locus_tag="ON430_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR3218393.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(24412..24885)
                     /locus_tag="ON430_000447"
     CDS             complement(24412..24885)
                     /locus_tag="ON430_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR3218394.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(24954..25109)
                     /locus_tag="ON430_000448"
     CDS             complement(24954..25109)
                     /locus_tag="ON430_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HCR3218395.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(25243..25623)
                     /locus_tag="ON430_000449"
     CDS             complement(25243..25623)
                     /locus_tag="ON430_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3218396.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(25627..25842)
                     /locus_tag="ON430_000450"
     CDS             complement(25627..25842)
                     /locus_tag="ON430_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218397.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(25845..26252)
                     /locus_tag="ON430_000451"
     CDS             complement(25845..26252)
                     /locus_tag="ON430_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HCR3218398.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(26518..27993)
                     /locus_tag="ON430_000452"
     CDS             complement(26518..27993)
                     /locus_tag="ON430_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR3218399.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(27983..28831)
                     /locus_tag="ON430_000453"
     CDS             complement(27983..28831)
                     /locus_tag="ON430_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HCR3218400.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(28868..29230)
                     /locus_tag="ON430_000454"
     CDS             complement(28868..29230)
                     /locus_tag="ON430_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218401.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(29231..29389)
                     /locus_tag="ON430_000455"
     CDS             complement(29231..29389)
                     /locus_tag="ON430_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218402.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(29530..30154)
                     /locus_tag="ON430_000456"
                     /pseudo
     CDS             complement(29530..30154)
                     /locus_tag="ON430_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(30151..30462)
                     /locus_tag="ON430_000457"
     CDS             complement(30151..30462)
                     /locus_tag="ON430_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218403.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(30506..30799)
                     /locus_tag="ON430_000458"
     CDS             complement(30506..30799)
                     /locus_tag="ON430_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218404.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            30993..31640
                     /locus_tag="ON430_000459"
     CDS             30993..31640
                     /locus_tag="ON430_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3218405.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            31701..32846
                     /locus_tag="ON430_000460"
     CDS             31701..32846
                     /locus_tag="ON430_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3218406.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
BASE COUNT         9552 a         6740 c         5548 g        11070 t
ORIGIN      
        1 gtatttatga aaaactacct atataaaaaa aacaagatat aacgatcttc gtcatatctt
       61 gcttcttcac ctaactattt ttctccctat ataacagctt tacgcttctc ctaatacacc
      121 attcaaatat aaatcgatca attgcgccac atttagcggt tcttccacag gtcttttcga
      181 ccgagtgatg taaggtgcta acactaagaa aaaatgcctt gctgcgattt cttttgaaat
      241 cccatttctt agcacagatt ggtttgctga aaagatttct tcaaaaggtt cacgataatt
      301 tttcttccat agaaagaaaa gatgatctct tgtttccttt gaaagattgc tttcaagatc
      361 gtgaagcatc agaaacaaat cgaccagatg tgtctcaatc aaaaactgcg ccatttttgt
      421 caaggttact tgaaatcccg tttcgcttgt ttgaacgatt tctctcaaag cttttccaac
      481 atcttttaag tggttttcta aaactgccgt atatagtttt tctttatcac cgaaataatg
      541 atagagattg ggttgggtga tcccaacttt ttgtgcgatc tctcttgttg aagtgccgag
      601 atatcccttg gacataaaca agtcagtcgc ttctttcaaa attttttgct gtgtatcttt
      661 gttgtatcga tggaccattt ttccacctgc tttttggtta ttctttttct ttcaatacac
      721 catcttccat cataaatatt ctatcgcatt tgtcaatcag ccgtgtatcg tgagtaacca
      781 tgatcgttgc tttatttttg tcttttgatt cttttgccaa gatatcgact acttctcgtg
      841 ccttttctga atccaaacta gcagttggtt catctgctag aataatggac ggatcattgt
      901 acaatgcacg agcgattgcg accctttgcc gttctccacc agatagttct tctggatact
      961 tgttgatcaa cttatccacg ccaagctgct caaataattc ttttgcttct ttttgacgat
     1021 gttcatgttt gatcttatct actaataata actgcttttt tactgtcaga aaaggaacca
     1081 gattggaagc ttgcaaaata aagccaattt ctttaaaacg tagtttaacg cgttcacttt
     1141 ctttttgatt actgaattct tgatcattga taaatacaga gccactgcta ggcgtctgca
     1201 atccgcctgc caaagtcaaa aaagtacttt tacctgaacc agaaggaccg atgattgcaa
     1261 caaattcccc tttatcgata ctcatggttg tttctttcaa tgctttaact acttggtcgc
     1321 catcttggaa acttttttct acttctttaa attctattgc tttcatgctc ttcctccttc
     1381 ttatccaatt gccttcaacg gatcgatttt tacgattgtc cgaacagaaa atagtgcgcc
     1441 taacacagct actaccagca tcaacaagct aatgacgcca aaaaacagcc aattgctttg
     1501 gaatggaacc gcatcaggta gaacaaggga tgttcccact gttcctaata gaccaagaag
     1561 aataccaaca aatgataaaa tgaatgtttg tgccactaca gaacgcgcaa taaatcctcc
     1621 tgtgatccct tgtgctttca ttacaccaaa gatattaatc ttttgcatcg tcagtacata
     1681 aatgaaaatc ccgatcacaa tagccgcaat aacgatcaaa aatccgatca taaatccaaa
     1741 agtcaatact tgcgcattat atccaggtaa ttcattgatg aatttagaaa ttttgatctg
     1801 ttctaaatct tcaggtaaat tactgatttt acctctgaca actattgcgt taatccgtgc
     1861 attttcactt gtatcttctt tttcgaagcg gatctcttgg taagcattga tggttgtata
     1921 gagaactggt gagacattga atttggcatg atccgtaaag ccagttagtt taaacgtctt
     1981 gtcactacca gataacttaa ctgtatctcc tacggctaga ccatattctt ctttcaaact
     2041 aatatcgcct actgcctcat catcgttatc aaaagctttt ccttcaacaa gattgggcat
     2101 gatgaattga tttttatcga taccgaagaa gctaacattg atttttcctt cttctgtact
     2161 gtctttacta gtcacgactc caggtgtctg ggcaagatac gccactttcc caccttcgac
     2221 ttcttctgct atcttcttag taatcatgga catccctaga tttgaatttg catctttcga
     2281 aagcacaatc gaatctgcct cccacttgtc gactgctgtt cgattatctt gagctaagcc
     2341 ataagctaac ccagtcaaaa aaaataccag gtaggcgatc aaaaacatca cccctgcaac
     2401 taacgcataa cgtaactttg aatgcataat ttcatttaat gctaaaaaca tttaactcct
     2461 cctcctgaaa taatcatttt atacaacaat tttatcactt gataaatttt tggcgaatat
     2521 tatgctcaaa aaattcttcc gctatttcat tatatgaatt ttttattctc gattgattaa
     2581 tatttattat atccccacac aataaaagac cgtgatattt ctcacagtct tttatctact
     2641 ctattgctat ttgctctctt agctagtact ttaaaagcta actcattgag gtagtgaaaa
     2701 cgagaggtgt gttcagacag aaatccaaaa tcctcaactg ctagctgtgg aatcgtagtt
     2761 ttgtgtttca cgatatccgc aagcaggttc aaatctgata ataagaattc ttggcttatc
     2821 agctgtccgc caattatttc taatgtatcc ggatggtaga tcagctttgc atttactgtt
     2881 ttgttttctt catcgtattg tggaaataga tcaaattctc ctgaacacga accggtattt
     2941 tgttccaaga atggcgcttc atcttctgtc agccctacag ttcctaaaaa gtagtctata
     3001 atgactgctg ctgttatctt ttgtctgagg tcgtaagcgg ttggctgacc actcaaagtt
     3061 agcgctactg ctcttgccat atgtacagct cgagtaacta aaggtaaaaa gagatgccgt
     3121 ttatttttgt caaaactgac tgggacgata tctccaatcg catagatgtc cggatcactt
     3181 gtctgcagat attcattgac tatcacagtc tcatcctcat acaaagccac tttttctttt
     3241 aacaatgtcg tattaggacg agtttgggca gaaaaaatga ccatatctgc ctttattgct
     3301 tgagatagaa gttgtacgga tactactttc tcttctgatt ttttccaatc gatcaagaat
     3361 tcatttggat aaaaattgat ttgcttagtt tgcagctgat tttgaaaact gttgatcata
     3421 tccttatcga aatatcttga taaaacattc ggcatataat caactagatg aataatgtat
     3481 cctttatttc tcaaagcatc tgctaattcc aatccaatat aaccagcacc gaagatcact
     3541 acttcttttg cttcttttaa agcggcaagt acttgcttag attgcactag atttttatag
     3601 gtgtagacat tttcagcttc aatttgttta tacaaggtga caggagagga acctgttgct
     3661 acgattaatt tatcataggt tatggtctcg ttttttcctt ctttggatgc gaggatgagc
     3721 tgatgtttgt ctggatcaag ttcagttact ctccattcgg tgatcagttt tgccccactt
     3781 tcttgaagag tccgcatgtt atgggcaact tcagctaaac tacgtatctt tccttttaaa
     3841 taaaggttga ttccatttga aacatagcct agtccatctt tatgatcttc atcgatcagt
     3901 aaaacttctg ctttcggttg aagttttctt agatttaggg cagctgtgat gcctgcatgg
     3961 gaagcaccaa tgatcacgat tttcatgttt tattcatcct taaattcaat ttttctaaat
     4021 aacattcttt taaaaattgg ttcaaatagg ggaaatcata attatattcg ttgctagtga
     4081 atacaaaaac tttagcggac ttctgtgacg ggtactcttt caattggcta gtcaaaatga
     4141 tatcatactg cttttctttc tcgtattcga ctaaatcata tttgatcgta ttcagcaatt
     4201 gttcctggat gatttctttc acgatatccg ccattagcct ttcataagga agatcataag
     4261 caatttgcag acgtaattct aactgcttga tcagcgtccg caaaatagat gcatagcgag
     4321 caaataattc aaagcgggca ttttcctcgt ctacttcagt aaaatcaaca atagcaaatg
     4381 tttgtctgac caatctttca cataaaacca ggaattgttt atcttctact tcagttaacc
     4441 gctgttttaa tccttcttgg ccaaaaatat tcatccatcc tctaaaatag gcaaaatgag
     4501 cgtgattttg catcactgtc aacgcaatct tttgcgaaat ctcttctgga aaaacataaa
     4561 aagagaaata ttcttctatc gattgtttaa acgttcgatt caattgagct actacgtcgt
     4621 ctttgtattt ttcgcttatc aaaaactcct ggacatgcga gttatgtgta tctaatgtaa
     4681 agttactgat catgaaaaga taaaaaacca acgtttcatt ttgattccaa ggaaaggcat
     4741 attgaaagaa gtaacgggtc aaaaatgtat ggaggtccaa taaaaattgt gtcgtttgaa
     4801 acaatccaac aggaagattg ctataatctg gtttggattt tttcgtacag cgcattcgct
     4861 tcttcataat ggaaagccat atttcaaatt tcaatcgtcc ttcttctgta atagtaaaac
     4921 taaactcttt ttgcagcaga tctaaaaaag tgtttcctga taaatcactg tattcttttt
     4981 tgttttcaag ataaggacgt ccaaaccaaa ataattggaa ataaaaataa caaatttgac
     5041 tttctggacc ggtcagccgc ccgcttttta ttttcagatg aaactcattt aaagaatcat
     5101 ttaattcctt cactctgcga gacaaagtag ccgaactgat attaagttcc atgacaagtt
     5161 tctgccgatc aaatgagcca ttttccaaca gataaagaag tatctgatat tttgtagaat
     5221 agcgcaagta gcaatgataa taaacatcga ctgctgctat atttccatgt ctgattaata
     5281 aaatattccc ttcttggata acaatttcca attgtttgat attttccaag ttagcttgta
     5341 aataaaacag atcttctttc aaggtagccc gatcaactga taagcgggta agtacgttat
     5401 taacggaaat ctctcctttt tcctctaata aaagttggaa aagtttgatt tgccgttgtt
     5461 catttttttc aagcaaactg aaaagttcca agtccactcc tccaagcatt tctgatttta
     5521 acagcagaag tttccttctc atccttgaaa aagaggatct gtgacagtta tcacagatcc
     5581 atacaaattc taatcactta gctggttggc ccagctgtca gttcccatgt gaccatgcct
     5641 tgatacgttg cgccaggttg agcattcgta ctgctattta cctctaagag taaaccatcg
     5701 gtatcgggaa agtcaacgag aacctctcct ttagctgttc catcactctc ataaatcaac
     5761 gtgttttgac cttgctgaat caattgtttt gatccagaat gattgaagta taattttgtt
     5821 ccgcttaatt cttgaccttg atcattctta aataaagaag tcaatgtccc tgtcagttgc
     5881 caaggagacg tgcttggaag ccgagtatcc cgcacatcaa aagaaaaatc gccttttgct
     5941 tcataataac ttgtcttcaa cggtttgggg agatcgttac caaatgtaaa atcatctggt
     6001 acattaacta atgttaattc ttgtttgggg acaagaattg ttttagcaga tgtttggaca
     6061 actgggttag gaagcttagc accatctgct aatgtaatat tgctggaaag tacagctgcc
     6121 tttgtcagta tgctgacgga ttgtacagaa gtatctacag tatttaattg atagttgtaa
     6181 gtaagatgaa tggtatctcc tattttgctc aatagatctg ctgggacaga aactccttgt
     6241 tgaatgccgg tatctgcgat agttgtacca tttaatttta aggaaccatt gatataagta
     6301 gtattttcag gaagtgagat cgtgattttt ttgtcggtta tcgcatcccc agattttgtt
     6361 aacgtgaatg tcacattttc ttgaatcgta tctcctgata gagcagacag gggatctcct
     6421 gtctcaggtt gtctgctaag gttttttagc tgactatctg cactcacaga tggaaaatta
     6481 gccactgtta tttgaggaag agtggtgatt ccagcttgtt cagactgcca tttgtacgca
     6541 aaagacggat acatgttagt aagtacatcg tccaccaagc gataatcaaa ataatattgc
     6601 cctgaagcca atgcaggtag tgtagaagta gctgtattta cacccccgtt atttgattgg
     6661 atcgtttgga aaagttgatc tgtccatcct gtcgctaatt tttgggttcc tttcgaataa
     6721 agcctgtaat ggatcgaacc cttttttagt tcagttgctg accaattggc cgtgacttgg
     6781 tacgatgcag aagaaaggag ctttccccac tgactatcgg ctgttaagtt tgttggtaaa
     6841 accgttccgt ctagcttgta ctgctccaat acctttaaag caacaggaga aagcaatccg
     6901 ccttgcgcat ctttcccact gtacacgata ttggctgtct gattcaatcc acttgtgctt
     6961 gctggagtat gagtcagtat tgctccttgt gtaacgtttt ttcctgtaaa atagttgact
     7021 acgcgatcaa agcgactaaa tggcaactga tcaaaacgtc cttcagtcag ccccatcgtc
     7081 aatgtccgac tattggtaga gcctggaagg ttctggttat ttccatccac gtattctgtg
     7141 gtatcagcaa atgtattata ttttcctgtt gaatcacttg ttgagatgta actattccac
     7201 gtatttatct ctgcacttgc agaaaatgca tatgctcctg gattcaagtt tccgccagtt
     7261 aatttaggat aaaatctacc tgcagctgga gctaaaatcg aattgaacat acgatcttgg
     7321 ataatattat tcgaatcata aaaatcaaca gaaaaaacgg cctttttaga catagaaggt
     7381 tgtaatttac ccttggtcaa aattttgttt ggactcgtat catatttatc tccgttttct
     7441 gtcatcttcg cgacattagc tgtaacatca aattgaagcg ggatatcgtc cacgttgaat
     7501 tcgcctcgcg taataatagc aataatatct gccaatagag tatttgtatc ccacgacccc
     7561 atcccaaaac gcaaggtatg gcttcctgct gataataaac taccgctagt aaccttgatg
     7621 ggggcaccgg caatatacaa gtaggcgttg ttccagtcaa ttgcttttaa tacatcactg
     7681 cttgatacat gttccggcaa attgatgatc gccacaaatg caaatccttg tgcagtgata
     7741 ttcaccaaat caagtacatt tgttagtttc acatggcttt cccaatgaaa acggaccgtg
     7801 ccacctttcc aaggatcatt tgaaccatcg ttcaaataag tagcagtcaa tttaggcaat
     7861 tggttagccc cagttagttg gacaagcttc ccgccattga ctgcactacg tccaccattt
     7921 ggaggtacga tattttcata cgtgtatgga tcataagaaa tggcaggagg cgttgctcca
     7981 acagcttctg ccgaaggtga aaagataaat atacttaaaa caaaaagcga agtaaaaaag
     8041 aaggagcgaa aaaatgaaaa tttattttgt agcaacatgt tcatcctcct tttcctttct
     8101 tctccataac aacaaccacg caagaagcca taattctata ccaatgatca aataaagaaa
     8161 acggatcttt tcttcattcg tctccgggaa tacgccatgt tgattagatt ctttataaat
     8221 cggtagctgt gtctgctatt ttgatgcgcc attgactgaa ccacttggat gcggaactgg
     8281 acttagatca taaaatgtaa tcgtacctga acttttggta tctgcttgga ctggctcttg
     8341 ccagctgaag aacagcaaac ttgcaataat ccctcccaat aagatcgatg ttttcaacga
     8401 aatcacctca tcatgttgtt tctccttttt tctttttctt gtggacccac aaaaacagca
     8461 aaatcaacca taacactaga actgcagcaa ccaatcctgc ataaagccat gggaaagatt
     8521 gttcttgtac tgcaaaaacg gaattttcat ttactttttg tgctttttct tttttgatcg
     8581 taaagttttg tgtaaatcgc cattcattac tgtcgcctga ttttgctatg atttctgcgc
     8641 ggtaatcgcc ggcttttgct ttatcaccag ctaaatcaag attcaagtga aaattcgaat
     8701 ttggtgcaac taaaaattca ttagacgtat tttcaatata aagctgattt tcataaaaga
     8761 tcttgctcgt aaatatcaaa ttgctgataa tcctaggctg aggattttgg aaacgaagct
     8821 gaacagttgg ctttgcgttg acctcgtcca aatctcctga taaaagtttc agatccggtt
     8881 ctatcgtctc ttgactttct tgtaaaacga ctgcgagatt ataagcaata cgatttttga
     8941 tttgtgctcc ttctgtagca ttttctgttt cagcttcttc tttaggtgaa acatgaatac
     9001 ccgctaatac acgtccctta aagctttttg ctgggatttt cacttctata ggtatagttg
     9061 tttctgcatg tgctgataca ttgatttcct ttttcgcaat gagcaaaacg tcacggacat
     9121 caaacggcaa tgtctcatct aacgttgggt ttttttgact ataatcgatc gttccaccat
     9181 cactcgttac cgccggattg acagagacct caaatgttcg ttcttcatct gaagaattgc
     9241 tgataattac ttctagtgtc tgattttgtt ccggtgccac taataaatca aaataaccga
     9301 ttgctttatc tatttggttc tctggaaata ccggtttgat tccaaagtct cctgctgaat
     9361 cctttgcttg gctgattgac ggaaaaatga aaaatagact taaaatagaa aaaaacgaaa
     9421 caaacagtcg tttactttga ttcattgtcc accacatcct ttcaacaaca actgcggtaa
     9481 aaaatccttc acctatagta ttttcaccgc agtttgttga atttatttca tactctatgc
     9541 tactgggcca gccacgaggt tccaagtcaa atcagccgtg aatgttccag ctgctgcttt
     9601 cggcactttc agtgtgatat ttgctggatc ataaacagaa agccatgttc ccagaccttg
     9661 atcttttgct gctgtaaata ttggtgtcgc tgcacttgct ttactcaacc cagttacagt
     9721 ttgactagtt ggtccagtgg attcgttatc tgacgtactt ttaacagtgg atgttccaaa
     9781 atctaattca gcgccttgca aaacttgtga accgtttaca aaatctgaaa ccgaaactgt
     9841 cacgttccac ccttgtgctt ggccatctgc acctacacct cgacgatctg atacttggag
     9901 ataaggtgta tcgttttttg aagcgtctac ttgatacgtt tttgttccgc tttcaatctc
     9961 gtgtgtacca aatggcaatt caggaactac atccaatgtt aaagaacctg tctggccagt
    10021 tgctggattt tccggatctg ctggctctac tggttttgaa ggatcagtag ggttaagagg
    10081 ctcaacggta tttgttggtt cagtaaaggt aatatctcct tttgaagtcg cttgaccctc
    10141 agcaaaagct ggtgttgcca agccgcctag aagaagtgtt gaaagtaata atgtcccaat
    10201 catgttcttt ttcataataa atcactcctt tttcttagtg acctaagtat agtagagaca
    10261 gtatgcaaaa aatcctcatg aattcatccg cttacttgaa attatgaccc tttttgtata
    10321 aaagagaaaa aaacaataaa atcgtttaca tgaatattct tcctttttta ttaagtatga
    10381 aaaaatattt caattttatt ttttcgtttc aaattattct attagtgaca tccttgaact
    10441 ataaaaatag gagtacggca tcatctgccg cactcctatt tttttcgtca tttatttctt
    10501 aaataaaacg attctcacgc aattttattt ttttgatatc ttctactgtt tgtgttccag
    10561 ctaattgcat gaccatttcc aattctgttt taaagaaatc aaacacttga tggactcctg
    10621 tacttcctcc taaagacaat ccataaataa cgggtctgcc aatagcaaca agatcggcac
    10681 ctgatgcgat tgctttgaaa acatgttgtc cacgtcgtac accgctgtca aacacgattg
    10741 gtacacgttt atctactgct tctgccacat attgcaacga atcaaacgca gcaggccccc
    10801 catctaattg acggccacca tggttagata cccaaattcc accagcacca gattctaatg
    10861 atcgatagac atcttcttct gattgaacac ctttcacgta gactggcaaa tcagaatgag
    10921 cagcgataaa ttctacatct tttggactaa gtttttgttt ggaagattta tatacagcat
    10981 ccatcgtttg gcctactcct gattgataag cctgaacgat cggcattggc agcgggaagg
    11041 taaacccgtt tctgcggtct gtttcacggt ttccgccaac tgttgcatcc gctgtcaata
    11101 cgattgcttt tgctccatta cgcttggcca cttcaagaat atccaaattg attccattat
    11161 ctttgctcat ataaaattga aaccattgcg gtgctttctc cccgcctgct tctcttatct
    11221 cttctaaagt acaagaagcg taagaacttg ctgtatagat cgtcccaaaa tctgctacac
    11281 cttttgcaga agctttctcc gccttcacat gagctaatcc atgagcagca actggtgcca
    11341 tgatgattgg tgcagtcagc atttcttcgt caaaatgtgt agttgtatcg ggtaattcta
    11401 catctcttaa tacatgagga ataatcaaac gatgattgaa tgcccgttca ttttcttgat
    11461 aagtaaataa gtcgccagca ccactgctga tgtaaccata accaccagct ggaatcactt
    11521 gtgaagccgc gatttctaaa tcttccatat tgataaaatc tactattcct tcatttgtac
    11581 ctgcttgata tgttttttcc atctcaatgc cttctctcta taaaatttga atgatgaaca
    11641 atatgatagc gtttacactt tttatattac tcttcgacat tgatacaggc aaataatctg
    11701 tttacatttc agccattcca gatgtcacta ctctggaaga aatgattgta ataaaaatag
    11761 ataaaaaacc gatgggactt tgacaaaagt agtgtcaaag tccctttttt tgaataaacg
    11821 gtgttcaaaa gccattttct cggtattaag tcaaatttat gcaaaaatgt ggaaagccat
    11881 tttcacaaaa tttttcttac tactcgaaac taagcgactt tttcacaacc tctcgataaa
    11941 acggtgttca atcagctgac cctcggtatt agctcaaaaa atggcaaaat atgaggaact
    12001 attttgccat ttgtctctct aatactcagg tcagaacgtt gacttcacag tctcttcctt
    12061 aaacggtgtt caaaaacttg catctgcggt aataagccca accaaacgaa aaataggagg
    12121 agctattttt gtttggctgt tcttattact tgatgcaggg caggtttttc acgacctctg
    12181 attcacggtg tttctttttc atcaacattt ttacgaaata ctgtggaaga ctattgaaat
    12241 aagtgtggat tgtttctttg tgtttcaatc gtttcttgcc attcttcatt taattttgga
    12301 ttaccgtttt cttccttgaa ctcataaggt cctaattcaa aaatcccaga cagtatttga
    12361 ttcacgattt catatagttc agtgtgatca gacacatgat tcttgaagat catctcatta
    12421 tcaccacgtt tgaacctcac attcgggctc tcatgatgaa cctctatgca tttttctcct
    12481 ttttttaact gacggacttc tgaaaacatc tcataaatat cttctccagt aaaaccttca
    12541 gcaagcgcca gactgtccac aagaccgcct actgtcagtt cagagattat tgcttgattg
    12601 gaatctaatt ttaatgtata gcttgtcgct tctactacgc cacatgcatt gcaaccgcct
    12661 tctgatagtt cgctttttct aatcaattga acttctttca ttttctcttc ctcctcttaa
    12721 ttttctactt atgtattttt atcacctgac ataggtgact cctatcaacg ctatcatgtg
    12781 cttttttcct cagaaatcct atttatccat tccatctatc ccttattctt ctacttttct
    12841 ttctctagca tataagagat acgcatagta ttcaagcaag gattgtcgat ataaaaagaa
    12901 caaatactgc tattttgatt tcttttggct atattacata taaataaaat ttatcatacc
    12961 tgctcttttt gactatatca tcatctgact tctatacttc gtttgtatta aaacttagga
    13021 ggaatcattc atgcttacag ggttaaactt attaaataat ccttttctaa ataaaggaac
    13081 agcttttaca aaagaagaac gggcaaaata tggaatcact ggtatgcttc caagtactgt
    13141 ccaaacactg gaacaacaat ctgttcaagc ttatggtcaa tacttaagca aacaaacaga
    13201 tttggaaaaa cgtatcttct taatgaatct attcaatacg aatcggacat tgttttataa
    13261 attgatgggc gaacatttag ttgaattcat gcctgtcgtt tatgatcctg tcgttgcgga
    13321 ttcaatcgaa caatacaacg aaattttctt gaaaccgcaa gatgctgcat ttctttcaat
    13381 cgacgaacca gaaaacatca aagcaagttt gaagaacgcg gcagatggac gagacattcg
    13441 tttgattgtt gtgactgatg cagaaggaat cttaggtatg ggcgactggg gtgtgaacgg
    13501 tgttgacatc gctatcggca aattgatggt ctatactgct gctgctggta ttaatccagc
    13561 acaagttttg cctgtatcga tcgacgctgg tactaataat gaagaattat tgaataatcc
    13621 cttatactta ggcaatcgtc atgctcgtgt agaaggcgaa acttactatg aattcatcga
    13681 tcaattcgtt caatcagcta ccgaattatt cccagaattg ttattgcatt gggaagattt
    13741 tggacgagga aatgctgcag cgatcttaga aaaatacgaa gatcaaatca ctactttcaa
    13801 cgacgatatc caaggtacag gaattgttgt cctagcaggt gtattaggcg gattgaatat
    13861 atccggtgaa caattgaaag atcaaataat tttgacattt ggtgctggta ctgctggtgt
    13921 gggtatcgca aacatccttt tagatgagat gatccgtcaa ggtgtacctg aagaagaagc
    13981 acgccaacat ttctatcaag tagataaaca aggtttattg tttgaagata cagaaggatt
    14041 gactcctggt caaatcccat tcgctcgcaa acgctcagat ttcactaatg ctgatgaatt
    14101 gacaaactta gaagcagttg taaaagcgat ccatccgaca atcatgattg gtacttctac
    14161 ccaacctgga gcattcactg aagaaatcgt gaaagaaatg gctgctcata caccacgtcc
    14221 gatcatcatg cctttgtcta atccaacaaa attggcagaa gcaaaagcaa aagatttaat
    14281 agaatggacg gacggaaaag cattagttgg aacaggtatc cctgctgctg atgttgaata
    14341 taatggtgta acttaccaaa tcggtcaagc aaataatgct ttgatgtacc caggtttagg
    14401 acttggtttg attgcttcaa cagctacacg agtaaatgct gaaatcattt ctcaagctag
    14461 tcgagcatta ggcggaatcg ttgatgtaac aaaaccagga gcagctatct tgcctccagt
    14521 agcaaaaatc actgaatttt ctcaaacgat tgctgaaact gtcgcaaaat cagttgtcgc
    14581 acaaaacttg aatcgtgaag aaatcacgga tatcaaagaa gcagttgaat ctgctaaatg
    14641 ggttcctgaa tataaatctt tagaagatta agatcgttat agtaaaagaa catacagaaa
    14701 ctttgtatga cgtctaaaaa gaaaaaagcg actgcggcaa aacagcggcc agccaactcc
    14761 tgctcttttg tcgcttttct ttggcttttt tcatatgata cagaataaga aaggatctgt
    14821 aaaaatggca caaaaaacaa caactgcaac tgcagttgag gaaaagaaaa aagaaggttt
    14881 ttggtccaca aaaatcaccg gcgtaagttt gccactttat ttgatcatgg tccttgtcct
    14941 gatcattgca atggctttag ggaaattacc gacgaatatg attggaccaa tcgcagtttt
    15001 ggttattttc ggtaatttat ttcattttat cgggacaaaa atccctatcg tcaaaagtta
    15061 tttgggtggt ggttccgttt ttgcgatctt cgcttctgct gcgattgcta catttgggat
    15121 cttacctgaa tatgtagtga aatccacaga aaactttgtc agcaatatgg gcttttggga
    15181 cttttatatt gctgccttga ttactggtag catccttggt atgaaccgta atctattgat
    15241 gaaagcttct gttcgtttta ttcccgttgc acttatttca atggtcgtct ccttttttgc
    15301 cgtaggactc gtgggcatgc ttatcggaaa tggttttgca aattccgttt tatacatctc
    15361 attacctgcg atggctggtg gtgttggagc cggagccgtt cctttatcaa cgatttatgc
    15421 aaatgtttta ggtacggatg cttcttctat catttcccgc ctgattcctg cgacagcaat
    15481 gacaaatgtc ttagctatca tcggtgcagc attagttgct cgtgctggac aatcaatgcc
    15541 aaaattcaac ggcaatggta aattaatgga aaaaggcgat cttggcgatg gaaaacctcg
    15601 aactgtgaaa ccttccattc cacagttagg tgttggtttg atggtatcat tgactttctt
    15661 catcttagga caaattggta actattttgt tcctaaagtc catgcttacg catttatgat
    15721 tattatcgtt gtcatctgta aaatcgccaa cctattacca gagtattatg aagatgcagc
    15781 tatcatgttc aataacttga ttgtcaaaaa cttgacagca gctgttcttg ccggtatcgg
    15841 tattgcgttg ctgaatctga atgtattagc ttctgctttg acatggcaat ttgttgtctt
    15901 atgtctgaca agtgttatcg cgatttcagt tgtttcagga tttgtcggtc gtttgtttgg
    15961 tttatatcca attgaatcaa ctattactgc tggcttatgc aacaactcga tgggcggaac
    16021 aggaaatgta gccgtcttat ctgcagctaa tcggatggaa cttattgctt ttgctcaaat
    16081 gggcaatcga cttggtggtg ccatcgtttt gatcatctct ggtttcttga tgcaattact
    16141 ttcttagtca aaaaaacaaa aatacctgat catcaagtat cggcttggtg atcgggtatt
    16201 tttttgataa agatttacag gattttcaaa aatctttttt gcattttatc ggaatgataa
    16261 ccgatgtgtt cataaaattg gtgtgcttct actctgtatt ctgcagaatt aagccttatt
    16321 gtcgttattc ctcgtttttt cgcttcttct tctacgccag tcatcaaagc ctttccgatg
    16381 cctctttttt cagcatattt agacacagca agtgccataa tattgaacat tggatcgcta
    16441 aaaatcgatt cataggactc cgcttgtaca tagccagctg cttttttaga agtaggatct
    16501 tcataaacca agaaaaaatt tttctctggc tccttcagca gtttcttcat ttgtctcacc
    16561 gtaacggtta atggtatatc ataccctaat tgatccgcat tgatttcacg tagtgcttct
    16621 gcatcttcta aacgcatttc ccgtaacatc caaatcacca gccaatttct gattttttaa
    16681 aatgaatttt tttatcatag tatactcttt ttttcattta gaacaatatg tttcagtgat
    16741 tttgacttgc gttagtctag attctcctaa tgtttgtaag atgacaaagg cattgtaaca
    16801 attgaccaaa agaaccacga ggacaattac ttgaaaaata aaggtcactt tttttggaga
    16861 caaaaccctg ctaaatcttg ctccccacaa acctccaata acagcagcgg ggataacata
    16921 aaacaacata gataaatcaa agcgcataaa gctacttgtt aaagcaatcg ttaccaattt
    16981 tgccagttga gaaaagaaaa tcgtagatag agaatatagt gttgcttctt ttattggcaa
    17041 tgcaaacatc agcatcaaca aagaaacatt gactggtcca ccaccaatac ctaaaaaact
    17101 agctaaaaag cccagaaccc ccccacatat aaggtaccaa gtccatgacg tcaaatgaaa
    17161 ttttggctga tgatgcttgg tgtagaagaa cgcaaaaacc aaggtcaata ccgtcaaaaa
    17221 aatttggatc agctgcacat gcttctcgtt ttcaaataac tgtaaaaaaa cttcgaatac
    17281 aatgttgccg actactcctc caacaacagc tccactagat acccaaccga caatctgcca
    17341 attcagtgat ttcccgcttg atacttgtcg cacagtcgaa acaatggaca tcgtgaatac
    17401 cgcaactgtt gagtaaaaag aaatccctgc aacagaatgg gcgccgataa gatcaagaat
    17461 cggtttgatc aacacgcctc cacccatccc ggaaatagcg ccaactgtgt tcgccagaac
    17521 gatcaccaca aaataaatca gaccaatcat cttgtatttc ctttctcttg tttatatctt
    17581 ttagcgattg ttactctttt cttttattat acagtaaaag aaacaaacga ggcttcgaaa
    17641 gcttttctac tttttaagga aatttagtgc taaacgaaaa gaggttgtga aaaagctgtc
    17701 ctgcatcaag taataagaac agccaaacaa aaatagctcc tcataatttt cgtttggttg
    17761 aacttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggtc gtgagcctgc
    17821 cgttcagctc cgagtcacaa ggaacaattt caaaaaacag cttctcatgt ttgttgaata
    17881 ttgtgacttg tgaccgagga gttggcagga gaacaccgtt taaggaagag actgtgaagt
    17941 caacgttttg acctgattat tagagaaaca aatggcaaaa tagttcttca tatttcgccg
    18001 tttttcgagc gaatgccgag gggcagctga ttgaacatcg tttatttgga aaaagggatt
    18061 gtgacaagac gtttgtcaca atcccttttt ttatatctta ttaattcatt cttttgttct
    18121 attcttcttc ttccgccatg aatgagaaac ccatatcaaa gaactataaa ggttatcaaa
    18181 acagcgttta tgactaaatg aattagagtt atctatcact atttttcaag cgatttaaac
    18241 caaatggcat acaatatggc atacaaaaac ttcaataagc cgtgggggtt tttaaatttc
    18301 ttcttgttct ttaaaatcaa tgagtggagc tacataattt gaatccactt tttcaaacgt
    18361 cactttttgc atcgacgaat gttttctttc ccaagataac gtaaatagat ttgactctgg
    18421 acttataaag tctacttcac cttctagtat tactttctta tgcaaatatt cctctaataa
    18481 atatagactt gctaaagcat acaacacatt ttccaaattt gcttccttat aatttgaaga
    18541 acgaccatgt tttactctgt tatatatttt ccaccaatct ggtgtatctt caggccaccc
    18601 atctccaaaa ggttttagtg aagtcaatgt taaagaattc ataaccctaa caccattatc
    18661 caaacacatt aattcgctag atttaataac agctttaaaa tcagttatgc ttttattgct
    18721 actgctatca aatccgcaaa gttctttgaa aataatctca cactccgaac caatcgctag
    18781 tagtagtttt tgatattcaa tagagaatgt tttgaaatta ttctcatcga gcatcacaaa
    18841 atctgtggta ttaataaact cattttctaa atgtagataa tacttccaat aaaaatttgt
    18901 aaactctgct ttgtccatta aatcacctct tttgaattat aacaaaaaaa acacgtccac
    18961 aacagacgtg ttctttgatt tctacacaat cagcttgcca aaaccatcca atcagtccta
    19021 gcatgcctgt attaaaattg aacgttcatc atctatcatc tttcttagtc cagcatgttt
    19081 gcaaccacac ctacaatttt cagtccaaca atagatttcg actagctaag tcttgcaaaa
    19141 ttcaggcagc taacccttca tttactacct taattatact aaatacccac aacgtttaca
    19201 agtgtaatca ttctaagaaa tctgtttagc tatgatcgtt gtataatctt taaacgccgt
    19261 tccttcattg attgaaataa ttttataact cttgtttttc caagctactt tcatatcgtt
    19321 tgttagttca taatcttgat cgtatcgaat aataaacgtc aacgttcctt caagaatggt
    19381 acctacacta gcaagcacgt cttttagcat ttgtgaacgc acattagccc aacattcaaa
    19441 tacagtttct tcactatcca ccatctgccc atcttcatct tttacccgtt ttgtcttttt
    19501 aaacttgact ctctcagtca attcatttac attcttaatg attggcataa taagcacctc
    19561 tcatttgttg tactaatgct tgtaccgtta ctggtatccg ttcgctgctg gcttcttgac
    19621 ggttcaaata ccaatgttga gtaagtaatg acaccgccaa atcaaattgc tgataattat
    19681 ccatatcttt atccgttgcg ctactatcaa tagcactttt tatatattcg cctgctgtat
    19741 caatcaattg ttgaataaag tcatcatcaa tagtgtgatc gatccgcata ctttttttta
    19801 tgctatccaa aattaccatt ttatctctcc taatgaaaaa aaggcagtgg gataaaccgc
    19861 cgcccaatat ttttctatac ttttggtgtt acaactggcg taaactcaat atacactgaa
    19921 gcattttgat caattttctt ataatcgttg cgaacgatca ctgcaagtcc ttgtgagtag
    19981 taatcgaatt tttcccattg agtcgttact tgatttctac gagcaacaaa gatagattgt
    20041 gcaatatccc ccataatcat agggaatgtt ccagctttag gattggcaaa caatgtatct
    20101 gcaatcaata ctactggcat accgaataat gatttaccac taggtgctgt cacatcgggt
    20161 tgtaaaatgt aacgtccatc tgaatctttc aatgtatcta ggtggtaata tccgctttgg
    20221 tttagaatta ccattttatt taatgctggg tctaatgcca cattgtatag ttgtttcaaa
    20281 tcgtccaacg tagcagccgt tttcttagtg aatgttttta ataaatccat gatatgctta
    20341 ttgtcagtgt tttctaccaa tttcgctaac tgatctttga cctcttgtac aatattcact
    20401 gctgaatctt ccacaacctc atttgataag gcaatcttac cagcacgagt ttctacttta
    20461 taatcaactg aagtaaacat ttcagcgtca atatcaccaa tttcagcaag ttcagctttt
    20521 gttgctaaca ctgcttgttg gttagttgct actggatatt taccttgtcc atttgatact
    20581 gtttttactg ttgcatattg agctaagtta taatttgaac gtttcaaatc aaagacttca
    20641 ccgattactt cttcgggaac aactgccgct gcatttacag tagtgactcc atcacgcact
    20701 tcgccttgtg aacggatata ttcttcatac ccacgcaatt ctgttttttc gttatcgatt
    20761 aaagtttttt ccatgttatt actctccttt tgtgctttag aagtggattc taagctattg
    20821 tttgtttgtt ttgcttggtt gttactcata aacgattcgt aagaacgctt atttacttgg
    20881 acatttgatg aatcataagc gggaacggtc acgacactaa tttcattaag tgctttgatt
    20941 ttatctattg atcgttcaat tgtgccatct tcttttttgt cgaatgaatc taatccaagc
    21001 acgaacccaa aggacataga atcaacaacc ccttttgaga tattctcata cacatcattt
    21061 gaataggtcg tgtcggttag ctctgcttca aaatgtaatc ctgtatcatc gacattcaat
    21121 ttcaacgtac ctgctttgac actagctagt ggctttgaat aatcgtgtcc atataataag
    21181 aacacatttg ataaatccac ctcttttaat gcttcgggtg taatcacttc tacaaagccg
    21241 cctaaatctt ttgacggttg cccgaatttc aacgcatagc ctgaaatagt tttgccgtct
    21301 gttggttgtt tttcattttc tttctctact ttgggagaat cggctgttag ctcagcttct
    21361 tctgtcagcc gtttttcctt ttcttcttca ttattcaact ggtgtaactc cttcctgttc
    21421 ttttaattga tatttctcca acgtatctaa atacgtaaag ttcaaacttg ctagtaaacg
    21481 atcgccacca tccatggggg gtagccccat tttcgaccgc ccctcattaa tcgtgagaag
    21541 tgaccctttg acctgatcca atacattttt tacttttgtt tcaggatcag tctctagtaa
    21601 ctgatcggaa ttaaagcgga tattggtttc aagtttcgta tctaattcac tcacaaacac
    21661 attaaagtaa tgaatcagcg tactttggat atactggaga ttactttgaa cggtgctaga
    21721 atgttcattt tcaacgccta aacgctcaat cggtacacca aacgccttgg caatttgttt
    21781 tgtgttccaa tcattggaat tgataagctt caaaacatcg gtgtttattt ctaatgtttt
    21841 gtagtccatt gtttcatcaa gaatgatcgt tctaagagca ttatctccac tacttgaacc
    21901 gttggcttct tcaaactttt cacgaattgc gttctttgct gatccgtcta aatctgactt
    21961 atgaaccttc aaaatacctg acccactgac accacgagta aagaaattgt gcaatgtgcg
    22021 gttgccagct tgttgaattt tcagttcatc acgtaacgca taaagtggtg gtaatcctgt
    22081 taaaccatct tgggtgaaat atttgaagtg caaaatatcg ctagacttca cacgtctttt
    22141 tttatcacca atctgataag acaacgtacc attatctaat tgagtaaccg ttacacttga
    22201 gttaggtaat aagtgaattt cttctacctt ttcaccgttg cgtttaatct ctgcaaagct
    22261 attaccgttt agcaacatat tgaccgccaa agcaaacttt aaatgccaac catccatttc
    22321 tgaattaggt ttctcgttta gtaacttcac cagttcatta tcagcttgtg gcatattgtt
    22381 tttgaccaat tgaatcggac ttgaagcaat atcactagca ataattcgaa cggctgcaaa
    22441 cacatcacta tttcttaatg cacgaacgct tgtaaagctg gtggtgtaat catccgattg
    22501 gattgatacc acatgttgta ggaaaggatc tcccgttgta ttttgtctcg ctttaaaaaa
    22561 tgccatcttt taacctccct tctgttctgt atttagcatg accgctaccc caataagcac
    22621 tatgcccagc gctaacaagc catagtagac atttgttagt aaggtgatag caaccaacac
    22681 acacaccaat ccaagaagta aaagaacggt atgaatgtaa ttagaaagaa aattcttcac
    22741 ttgtataaaa ttcattgtct gctttcgctc cttccgttgt ggtgaagtaa tccattgcaa
    22801 acacataggc attgattaat gcagctattg gatcaatttt gttactgttc tttgatttat
    22861 taatctgaat gccgttgtta tccgttttta aaatagcgtt gttcactgca tgggtgagaa
    22921 tcgtattttt attgtgaata acgttgcctt cataaacttg ttctctaaaa gttcgggtcg
    22981 gaacgtttag agtaatcgtt ccttgtctta cttctaacat tgggtagttg gctttttcag
    23041 ctttactgat aagcgaatta gcgttatacg ggtcataaca aatccccata cattccaaat
    23101 cattttcttg aatcaaatct tgaataaact gaaatacttg gtcatagtcc acgattccac
    23161 tttctaattg agtaatggaa cactcaccag ctttttctag ttctcgataa ggcaaaccgt
    23221 cgcgcttttc tttgtcttga agcccatatt tggtggctac aaagctatgt gaatcacaat
    23281 aaagttcgcc gttatctagc ggtacgatcc atgaaacgct agttaaatca tctgttttag
    23341 ataaatccac tccgatataa acgggcttgc ctgtaatatc ttgtggttct acttcggttg
    23401 cctgccagtc ttcagcaatc atatagctgt cctcactcgc ttgtctccat aaattgaagt
    23461 ttttcaccaa tacagaatta aggttatttt gtttcaaagc aaggttcaca tcatcttgaa
    23521 tggttggaat cattactttt ttgatttctt cactttcaaa aatcggattg gctttgatcc
    23581 atttctcttg atcgtgaatt tcttcgggat cgtccaattc ccatatcgct ataaagtacc
    23641 tgtcggcttg ttcttcgcct tttagcacac gatctaacaa taagtattcc tcgtacatag
    23701 gaacgtttaa atcaagccca gcggtactaa taacacacaa taagccgttc ttttgttggg
    23761 tcattcctga cttaatgacg ttgtacgttt tacgagtttt tgcttcgtgc cactcgtcaa
    23821 gaattgcggt tgttggtgca aacccatcta gcgtgctggt ttcactagcc agtgccatag
    23881 cgaacgaatt agagggcaag tgctgaatct tagagttcat aattttcagt tgtggtctta
    23941 aaaacttact agacttgacc acgtttctaa gtgaattaga cagcatatca tagcccaatt
    24001 tagcttgttt caaagcgtta cttacaaaca atacttgcct tgcttctgct ggttcttgtt
    24061 ccataatcaa cgcattggca gccatgccac ttgcgatata cgttttccca ttcttacggc
    24121 tcatactggt aaacgctcga ttgaatcgcc tgtaattccc ttctttagta cgccagccat
    24181 aaagactgcc tagaatgaat ttttgaaatc ctaacatctt gattgcttta ccgtccgtag
    24241 atggtagcaa ctcaatgaat ttaatcgctt tggtggcttg ttcttcatcg aacacataag
    24301 ggaagctatc tgacttagaa cgctccaaat cgcttatatg acgctcacac gcttggataa
    24361 ttttcttggg tgcaatcagc ttacctgata ggactttctc aatgtaatca ttcatgaatt
    24421 catcaactct ttgaacgggt cttcgggttc atcattttta accttgttca aagctagttt
    24481 tgatcgtgat tctaaggtca tacctagtga ggtggcttgt tttttcaaat cactcatagc
    24541 ttgtgattga acccgaaccg ctggattggc tttctttact ccgttttcgc atgtgataaa
    24601 cgtacctgat tttcttatct cggcttcggc tgttttcatt cgagcaaagg ctaaacaata
    24661 ggctaccaat gaaccataat ctgcttcact caatggataa tcttttttta atgttggtac
    24721 gatacgattc cattcacttc tagcgctcgc tgccatccag tcagggggcg tgatatttat
    24781 taatggttgc tgcttgaaca attcttctct agcgtccaca cggattgctt tttcttcact
    24841 tgatatattg ccttttgtat cgtgtaatat tttagggtgt cccatgttgt cctcctttca
    24901 gacaaaagaa aaaggaacgt aagcaattaa gctcacattc cttcgttttt cgatcagaat
    24961 ttttctttat ctaatttatc tatataaatg atttttccgt tctgcatttg aatggtcact
    25021 tgaccaaaat gaggtaactc aatggtgcgc tgtttccctg ttttatcagt aataacaatt
    25081 gtatttttca ataaatctcc tactttcatt atatactttt tcacttgtga ttacaagtgt
    25141 aatcgttatt tgtaattacc cttatgcctt ttcacaacgg ttatttgtac caaaatgagt
    25201 aggcatcgat tgtcagtaag tttcacacta tgccccctta actcaaaggg tactcttttc
    25261 tcgtctttgt ctttcattgg ctgtcttgga attatggtgt gcatggcata aagaacgaaa
    25321 attagtttca tctaaccgtc tattccaatc atcttttagt tcaattttgt gatctacaac
    25381 atcagctttc cttatcaagc cttcttctag gcaatcctca catactggat gatttaaccg
    25441 atacaagtat gaagcctttc gccatgttct actttgatag aattgaaaat atttaccttc
    25501 gctttgcttt ctttcttcac gcttaattgc cgtttgcttt gctttatgct tcccacaata
    25561 cttctgccta taatcaatca atgttttaca tccagcatga ttacattgtt ttttaactgc
    25621 catagattac caaccaaagc gatctcgttc gtcagaatca tcttgttccc attttgattt
    25681 gtattcaata taaggcgaaa ctatcttcaa gcacgttccg tctttaaaat taactttagt
    25741 gattttatct tcctcaaaac taataaagtc tacatcttct tgagtgtttg aatactcaat
    25801 gtgtttctca ccatcgaagt atctaatact ttccatatgc ataattattc tacctccaat
    25861 aaattagtta tcaattcatc atctgttagt agcaaagcca agaacttatc atggatgttt
    25921 aaaaacaaat tatcctcatt cgatgtaagc tctcgttgtc tcgctcgatc atataggtta
    25981 agcagacaat taaattcatc cattagctca cctgtatcat catagttgcc actatctacc
    26041 aatgatccca ctactagcga ttgacaccac gctactgact cctgcagcca atcaggttct
    26101 tcttccatta tctttaatac ttctggatca aatgtggaat aatcttctgt taccaatccc
    26161 acactaaatg ccagcgtgtt cattagaaac cgatccattt catctgtata ttgaaagttc
    26221 ggtgctttgt ttgttgaaat aatattgagc atttactttc ctccttgatt cgtaacacgt
    26281 ggtaacacct acgtaacagg tacccgttac cactcaatcc caacggggac aagggttcta
    26341 gcgttttggt aacaggtaac gggtaaaatc cagtaacatt attccccagc atattagcta
    26401 actatttcaa aagttttaag gaaataaggt gttaccccgt taccaaattg ctgtatccct
    26461 tggggcagta agggtaaact agtaacgggc taggtgttac tgacccgtta ctacccgtta
    26521 ctttttgtac tttccagctc gatttttatt ttcataggca tttgacttat tgtaaaatcc
    26581 tcgtcgttta tttctttttg gcagttgctt ccgttcccaa ccaggaatac tgctaataat
    26641 caaggcaatc tttttagttt ctgcatctga tcgaccattc aataaatcct ttgcttgttt
    26701 atcaaaaaca gcttccaaaa tatcagcggt gagtacgcta tcaactaaat aaaactcttg
    26761 ttgcatagta gctccattat agaaatgctg taaatttttc tcattacatt tactgtcagg
    26821 atagttctga taataagaac gtctcgccca cgctggagct tcttcccatt tatacgggat
    26881 ttccatttcc aaatatttag tgattgcttc tttaattgga ttctccatct tggcatcctc
    26941 ttgatagtct tttgcaattt ctaagacttc tttatcttca tcgggactaa aatagattcg
    27001 ttgtccttta tcaaaaagca cctttgcttc agctaacact tgaagaaaat attcatcctt
    27061 tgttttaaac ggattcatct ttggcttatt cttacaaggc aaaggataaa aacggcggtt
    27121 tcctgtatca tctttcaaat actgcccatc attcgacgtt ccaataaata cacattgcct
    27181 tgcccaagct atgacattcc gaccataagg aggtctaatt ttatcgatct tggcactgat
    27241 gaagtttttt atctgttcaa ttttagtttt gttcattgaa gataattctc caagttcaat
    27301 aattacattg ccttgaagct gcatataatc atctttcttt tcacctagcg aatctaatga
    27361 atcggtgaaa aaatcatccg tgtaaagtaa tgctggaacg gtactcttac caatcccttg
    27421 cggtccatcc aaaactggaa cgatctcaaa ctttattcct ggtttataga tacgtgcgac
    27481 tgatccgact atccaacgtt ttgttacttc tcgtgtgtaa aggttgtcgt ctacccctaa
    27541 aaggtcaata aacagtgttt ccagtctagg ctgttgatcc catttaactt tttcgatacg
    27601 ttcttttact gggttgaaaa cattctttct agccacattt actactgctt tgtgtatttt
    27661 gttgtcatca aaaactacat tgtagttttc ttcaatatat gccgttaagg catcctcaaa
    27721 actgtcttcc cattctccag cggtacgatt gatccaagga gaatctttta ataggtgaat
    27781 gtatccgcta aattcgttat aagcaattgt tccagctaaa ttgctatcat tggttaaaat
    27841 caattctgca ttcacaacac tgttactttt aatctgatcg tctttattgc atcttaatcg
    27901 tgacatccag gttttcaatt caatgatatt gtcttcacgc tcgctggctt cgggaaagtc
    27961 taaaatacta tctaatttat gcttatgagc catgagcttt ccccctcaaa tcttttttaa
    28021 tcatcgatcg aataatttga ttcacttctt tatcttttag cggtggttca ttgtacgcat
    28081 ttgctgtata aatcaattct gctaaagttt gattatctac accgtgccat agtaaccaac
    28141 cgcataactt agttaaatga tcgtttctac taccttcgcc agcgccttga acaatcatat
    28201 ctaatttttt tgctgtccct gtttttgcta cgggtcggta ttgagtcttt atttcttgcg
    28261 gtttttttag cagtaggttc actagccaat cgggagctgg tgcaatatta tttagagata
    28321 cgttttcaat aggttcatat gctctcccaa cacttcttga aataatcata gttggcgcta
    28381 ttggaactcc tttagtgata atatcaatcc cgcttaaact tttaccttta tcatcacgcc
    28441 cgaagatatc agcaaatgca cctgtttctt gttggataat tacaccatca ggaattttaa
    28501 agaagaaatg gattccttta cctggtgtga tttccgtata agtagaagga aatggatcat
    28561 acattgaagc aagtttttta taattctcaa cgccgtttcg tgtttcagaa tgttgatcta
    28621 ggtcaactac caccatttga tgatcttcaa gcattaaccc aatatttcta gttgcatctt
    28681 ccgtccacca ttcattaatt tgattagaat cgttagttgc atcattccaa ccactagagc
    28741 cttttattgg aattttagtt ccaggagata agggataaat tgaccatccc gacttttgta
    28801 attccgttgc tgcttgataa ttatttgcca ataaacctct cctccttttt caaaatactg
    28861 gtataaatca ttcagacacc tccatgagct tcaactgctc aagttcgtat ttcaacccat
    28921 cccgaacaaa ttcagcacct tttaattgat tctgatactt tacccatgta ttaaatgttt
    28981 tcacccttgc ttcttgcata tcagtaatgt ttaaccatgc ggttaaaatc tgatttttta
    29041 gctcgttgat ttctcgacaa attattttgt gttctgcatg ttttttgagt acatacaaaa
    29101 ctgttagttt ttcattgaat gaatttccaa agtaggtttt cccgatatta ttttctagtt
    29161 cagcgatccc ttgattaatt tcctctaatt caaggctatc gagttggttt aatagttttg
    29221 ttttgtacat ctacttatgc cctccagttc ttaattgata gtcgtactcg tcataagcag
    29281 tgaagagata taacgctaat cctgcaaaga tagctatttt tgcaattaat ggtaataagc
    29341 cgaaaattcc gccgattaaa atgcttccta taattaagtt tcgtttcata atggttccct
    29401 ccgatttttt aatatggtat aatcggaaca aagaatatcg ggttttatcc caattaagtt
    29461 gcctttatcc tgtccgccaa gactgttatg gataaggctt tttctttgtt ctcgatcatg
    29521 ttaatgctcc taagttgtct gcaattcagc agaacgatta taaattttct cactcactga
    29581 aaactgcaac ccatattttt ctttaacagt cattaattct actgtctcat ctaaaatagg
    29641 ttcacgatca accaacattt caggagtcat atctttcttt tttaccattt tggaatgacc
    29701 ataaatagtt gatacagctt tattggctat cacattcgct ttaatgtagt ctactggttt
    29761 tggttcagta atagcgtggc ttaatttagt cattgcttct ttttggtgtt ctttgtctag
    29821 catgcggaat acttgaaagc cttcaagtcc tgtggactgg cgtagttctt tgatgatgtc
    29881 gaaaatccag tcttgaaatg cctcggcttc ttttttacgg cttttgaatg ccatgcggta
    29941 aatatttttt ttcgttgatg atgtttacat cttgtgttct gcccaatgaa tcgatgacct
    30001 tactagtagt aaccccatct ttaggcaaag agtgaatggc tctagtaacc tgtttaagac
    30061 ctaacgcttc cgcaacatct ttcgccactg cccaccattc atcattaata tcgacaaatc
    30121 ttataatgtg tccgttccaa atttcagttt tcattcaacg tcctccagtt caagccctag
    30181 caagctcgct aacaactcaa cttgctgata caaaatttct tgatactctt caatagaaag
    30241 tcgttcttta aactctgctt gtactaaatc gttcaagcca cgcaatactc cgcttaccgt
    30301 tggttcttgt ggtagcaatt tttgatagtc catttgttcg tacaaacatt caaacgtttc
    30361 ttctagcgct ttgccttcca agtttgctaa ttctgccgct gtgtcttctg taagaattgt
    30421 tgataatact ttttcgattg ctttattttg tgtcgattcc attattattt cctcctatta
    30481 atccctttgc ccccacggct tgcgtttatt tttggtattc ttccataaag cgatcacatt
    30541 catcttgtga aattcttacg actccatcaa ctataatcaa ctttaaaccg ttaggaacat
    30601 atttctgcat catagtattg tatgaaacgt tcatatactt agcagcttgt gccttgttca
    30661 tatattttgg aaactggtac tttttttcta ataatgaaag taacgttcct tgagtttgct
    30721 ctacttgttg aacaactcga tcgtcaataa ttttaaaagc tggatcatca agcgtgagtt
    30781 tgtcaatttt taattccata tttatccccc ctcgttagca tacatttcta tacaaagatt
    30841 ttaaaaaaat gcaagaggct cttgaataca tttctatacc tcttataaac tcaatatacc
    30901 atcatacctt ttcatttgca agaattttaa catacttttt tgaactttta tgtttcacac
    30961 atatttaaat cgtgatatag tagagaaaat atatgaatgg tggtgaaaat gtggctagaa
    31021 aaaaataccc cgaagcagtg gagacaggac aaagaattag actggctaga attgaaaagg
    31081 gactctcaat ggaacaactt ggaggtaaat tatccccacc ggccagtaaa ggtgccgtta
    31141 gtaattggga aaatggctat aatcttccca ataacaccag gcttaaacaa ctttcaaagg
    31201 tattagatgt atccactacc tatctattaa acggatctta tacattaaga gatatgcata
    31261 tgatgcccga aattaagcga aatgtgcttg aaaagcaatt gaaaaaaact attgaaaaaa
    31321 ataactctat aattgattct aatctttcta atgctatcaa taacatcgaa gaagataacc
    31381 tgactgtctc acagaagatt tatttaacaa acgcaataaa atatcttaaa acaggatctc
    31441 gaattgaatt tcctgacggg agtaccccaa ttgcttatct cgcagctctt ctaaatacgc
    31501 ttttacgata tgactcatta attaatgatt cagaaaaaaa tacagatgag aaaaaagagt
    31561 atcaagaatt ttatgaaaat gatcttctaa aagagttcac agctcttgtt actgagctaa
    31621 ataaacacta ttcaaaataa taataaactg cctcctccaa ccgcccccac ggcttgctac
    31681 actatggagg gaaaactatt atggcaacat tcaagcaata tgaaactaaa aaaggtaaat
    31741 tttggctcta tgaagcctat cttggaatga acaaaatgac tggcaaacct gataaggttc
    31801 gtagacgagg ttacaaaaca aaaaaagaag cccagcttgc cttgtcacgc ttgcaagtgg
    31861 actacgataa aaacggtatt aaaaaagcga acaacgaaac gtttaaagaa gtttatgact
    31921 tgtggctaga aacatataaa accactgtcc gagaagccac cttcatgaaa actgaaatca
    31981 agtttagaaa gtggattctt ccaaaatatg gaaatcttcg tgtgaatgaa gtcacggtaa
    32041 aaaaagccca acaaattgta aatagctggg ctgaaactac tgaccaatac aaggtacttc
    32101 attcaacttc taagcgtatt tttagctacg ctatcaatct tgggattatt gagactaatc
    32161 cactagacaa cgtattaatg cctaaaagaa ccaccactaa gcataaagaa aaggtcaaag
    32221 tttacagtaa agaacaactg atcaccttgt ttgattacct aaacagcaaa cctgaaactt
    32281 atcgaaacga ctttgataaa acgttactac gctttctctt ttatagcggg gttaggatca
    32341 gtgaagccct tgcattgaat tggtcagaca ttgattttca agaaaatacg gtcaccatca
    32401 ataaaacact aagccaatcc aaaaatggct ataaaatcag tgatcctaaa actgattgta
    32461 gtgctggggt tcttccacta gatgaaagaa caatttctat attaaaaaaa tggcagatta
    32521 atcagcggaa atatatgctg acacttggtg taactgatcc gactatgatt ttttgtggta
    32581 tctataaaga actaatcaca caccacgcta tttatgcaag attgattact atttgtgaaa
    32641 aagctatggt tccatttctc ggtgtgcatg tagcaagaca cactcatgct tcaatgctct
    32701 tggacgctgg agctactatg gtagaagttc aaactagatt acggcataca aaaattagtc
    32761 agacgatcga ttgttacggt cacttggcaa aagaaacgaa agaaaagact gttgaaaaat
    32821 tagtacaaca cttaaatatt gactagatgg catacaatat ggcatacagt aaaaaataaa
    32881 aaaaggagaa accctatcat atcaaggttt
//
LOCUS       DAJSMI010000011        32588 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJSMI010000011 DAJSMI010000000
VERSION     DAJSMI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.011"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(30..1127)
                     /locus_tag="ON430_000461"
     CDS             complement(30..1127)
                     /locus_tag="ON430_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR3218407.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1235..3262)
                     /locus_tag="ON430_000462"
     CDS             complement(1235..3262)
                     /locus_tag="ON430_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR3218408.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(3407..4138)
                     /locus_tag="ON430_000463"
     CDS             complement(3407..4138)
                     /locus_tag="ON430_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3218409.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            complement(4654..5553)
                     /locus_tag="ON430_000464"
     CDS             complement(4654..5553)
                     /locus_tag="ON430_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR3218410.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(5563..5919)
                     /locus_tag="ON430_000465"
     CDS             complement(5563..5919)
                     /locus_tag="ON430_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3218411.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            6274..6570
                     /locus_tag="ON430_000466"
     CDS             6274..6570
                     /locus_tag="ON430_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCR3218412.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(7214..8167)
                     /locus_tag="ON430_000467"
     CDS             complement(7214..8167)
                     /locus_tag="ON430_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3218413.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(8190..8945)
                     /locus_tag="ON430_000468"
     CDS             complement(8190..8945)
                     /locus_tag="ON430_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3218414.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(8942..9901)
                     /locus_tag="ON430_000469"
     CDS             complement(8942..9901)
                     /locus_tag="ON430_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR3218415.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(9894..10844)
                     /locus_tag="ON430_000470"
     CDS             complement(9894..10844)
                     /locus_tag="ON430_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR3218416.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            10994..11446
                     /locus_tag="ON430_000471"
     CDS             10994..11446
                     /locus_tag="ON430_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCR3218417.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(11535..13802)
                     /gene="gshAB"
                     /locus_tag="ON430_000472"
     CDS             complement(11535..13802)
                     /gene="gshAB"
                     /locus_tag="ON430_000472"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCR3218418.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(13989..14987)
                     /gene="ftsY"
                     /locus_tag="ON430_000473"
     CDS             complement(13989..14987)
                     /gene="ftsY"
                     /locus_tag="ON430_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR3218419.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(14998..15807)
                     /locus_tag="ON430_000474"
     CDS             complement(14998..15807)
                     /locus_tag="ON430_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3218420.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(15816..19397)
                     /gene="smc"
                     /locus_tag="ON430_000475"
     CDS             complement(15816..19397)
                     /gene="smc"
                     /locus_tag="ON430_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCR3218421.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(19417..20103)
                     /locus_tag="ON430_000476"
     CDS             complement(19417..20103)
                     /locus_tag="ON430_000476"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR3218422.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(20451..22232)
                     /locus_tag="ON430_000477"
     CDS             complement(20451..22232)
                     /locus_tag="ON430_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3218423.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(22258..23172)
                     /locus_tag="ON430_000478"
     CDS             complement(22258..23172)
                     /locus_tag="ON430_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218424.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(23199..24161)
                     /locus_tag="ON430_000479"
     CDS             complement(23199..24161)
                     /locus_tag="ON430_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218425.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(24161..25105)
                     /locus_tag="ON430_000480"
     CDS             complement(24161..25105)
                     /locus_tag="ON430_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218426.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(25106..26110)
                     /locus_tag="ON430_000481"
     CDS             complement(25106..26110)
                     /locus_tag="ON430_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218427.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(26378..26620)
                     /gene="acpP"
                     /locus_tag="ON430_000482"
     CDS             complement(26378..26620)
                     /gene="acpP"
                     /locus_tag="ON430_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR3218428.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(26742..27743)
                     /gene="plsX"
                     /locus_tag="ON430_000483"
     CDS             complement(26742..27743)
                     /gene="plsX"
                     /locus_tag="ON430_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR3218429.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(27792..29828)
                     /gene="recG"
                     /locus_tag="ON430_000484"
     CDS             complement(27792..29828)
                     /gene="recG"
                     /locus_tag="ON430_000484"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR3218430.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(29945..31627)
                     /locus_tag="ON430_000485"
     CDS             complement(29945..31627)
                     /locus_tag="ON430_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCR3218431.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(31679..32041)
                     /locus_tag="ON430_000486"
     CDS             complement(31679..32041)
                     /locus_tag="ON430_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3218432.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            32311..32499
                     /locus_tag="ON430_000487"
     CDS             32311..32499
                     /locus_tag="ON430_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR3218433.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         9035 a         7004 c         5670 g        10879 t
ORIGIN      
        1 ataaaaattt actatttgtt atcttaacct tacttttctt tctcttcttt ttttctcaaa
       61 acaaacaaaa gtatgcataa taaaataaac aataagaaaa gtgaagctac taagatataa
      121 atccaattat tgtctttttt aatcgtaaca tccgtcgtgt tcaattcctt ggctactttt
      181 ccgtctattg taaaatcttt ttcaaatacc cattgattgg tatagttgat cgtagtattt
      241 cctttttttc tcgaaaattt tccattttca ttttcatttc ctaaaacagt cattgtaagg
      301 tgatattcac ctggggttag cggttgacca tttaaagcag ttggaaaaga aaaatttgta
      361 ttcggcgcaa tctgcaatcc ttctttttcc tcttgatata aaacttcaga atgtcctttt
      421 tttgtaattt ttgtttcaat aactacttga ttgatatagg ttttttgatc attttgaacg
      481 tttgctaaga tgacatttct tgcattgatt tgatctggtt taacttcatg aagagtaagg
      541 ttcggagcaa cttcgttcag tgtctgttgc atcaacaaag caataatata ggtgtattca
      601 ttctcaatcg ataagctttg atccttagtt tctgtttggt ccttttccgt ctggatttct
      661 tggaatgtga tcccaccagc caatacacca tcaaatcgct cattcggcat cctgacatga
      721 aaagcaacgg tttggacact tttaggtttt agcgtcacta tctgtggata gctcacataa
      781 tctaccagat caaagatcag gctcatgtct ttttcaatct cgttttttcc gtattcaacc
      841 atcacatttg aattggtggt tgcgctattc acagaaatat cgattttcac ttctttttct
      901 gtatcatttc tcaattccgc ttttacttct acttcctgat ccggatcaag ttgaagatcg
      961 aagtaagttt ttctcttgtc gatttgattc tctgaagtta tcggcgtcac tgaaaaatta
     1021 aatgcactag ctgacactga tatattgtta aaaagtgccc ctaaaataac taggagtgtt
     1081 ataaataatt gttgttttgg tctcaagaaa atcgctcctt taatcatatg taagagaaga
     1141 ctataacaaa atcttgtctc tgtgggacac aagagcctat ggtacctgtg cccttcttga
     1201 aactcaataa ttttgttata ttcttctctt cctgttaatt tcctgggaca tctgcaagta
     1261 tccaagatag tttcccctca aaggttccga ttctttggtt ttctacagga atatctattc
     1321 tgattccacg atgagctgta gaatctaaaa gagaggacaa atcgctttca cccttttgat
     1381 cactttcgaa aatcgtaaca gcggatgtcc ctataaccgt tgtattttca gaggtaacaa
     1441 aggaaagagc ttctggaagc tcaaaatctc catttgtaag aactttctct tgtttcaatt
     1501 gtaatttcca acttccaaaa gcccgttcat ctataatttt caacccacct gaaaactgtc
     1561 cgaattcact taatggtcta ctggatattt tatgttttcc aaaattgaca tattgaggag
     1621 cagttgagag ataaagtttg ccttttctgt aataaaaata gatatcctct gtattttcgg
     1681 agaagatgcc ttctgtattc gctgaatccg agtcaaaaac atacccttca atatcaatag
     1741 gagtttcttt atacttctct cctattttcc cagtgattgt tttcatttca gcaatatcaa
     1801 tctgactgtc tttatcttta taataaacat ggatatctat ctccttcagc tttgcgatct
     1861 ttaggtttat ttcattcgat tgctcaatca aattcccgtt ttcaaggtat tcaaaatgca
     1921 tagtgagtac tttttcagga attacttcct gcaaagttgt ctccaattcg attattaacg
     1981 tatctccagc aataaaataa tctgtcagat ttagcgtgac tcgatttccg gttatcaaga
     2041 tatctttggc atcaatttga tttccattga tcaagatatg ctcgggaata gttaattctt
     2101 gtgggaggtc gatactagtc tgcattttct tatattcaag aaaatccccc tcgaccttca
     2161 atgtatttct gatagttatc ttatcgtttc ctccagcttc agcgatggcc ccataatcac
     2221 tatcgttgac ttttacatca gctgttgatg tgacattcga tggaagtcgc gtgacagcga
     2281 cagcattttc ttgaaaaaaa gtacctgttg aagaagtaaa cccccaattt acttctgtag
     2341 aaccaaactg cctaatgaga tcaggtataa tccgagaaga gcgaccgaca ccttccaaat
     2401 cataagtaag acttctttgc tccgcgtccc atttaattat taacaagcgc catgtgccat
     2461 tagaaagaaa tgtaggagct accgtcacgc cagtatgctc tccagaataa ttattattat
     2521 ttggtttagg cgtgacaaaa gcgacatgtc catgccctct attatcagca gttatctctc
     2581 gatccattcg atcagaagaa ccactatttt tatacgtatc aaactcaatc gagagcgcat
     2641 tcgcaatgta aggttggcca gaacgagtag aatatgagcc aattcccata cctggcgatc
     2701 caataacttg actgggatta ctagccattc taggatcgcc agtcaatgta aaggtcatgc
     2761 catctcccgc tctatctcgt tgatttccta agtataaata agatttaaat gtaaaatccg
     2821 agagtaaact aatcttctta tttgaccaaa tagctcctct ttgagacctt tctgctgggt
     2881 tcaactgcag taacttccca ttttccaata tggacgtgct agtaccgata ggtgtttgaa
     2941 aaattttatc gatagaaata atattgggat cgtcaggcag ctcaaccgca gaaattggtg
     3001 catcttctag tccttgtttg tccgaaccct cagcttctac gttgtcagtg gtctctccac
     3061 tactcgaaat acttaaatcc tcactgcttt cttgtgtctg gttggattct attaaactgt
     3121 cgaatgtcga agtgagtttg ctttcttcgt ctcttctatt tccatgctcc aactgattca
     3181 attccaaaga gtaccctatg atcggaaaga tattagtcac taagagactg cttaaaatga
     3241 gcacgatcct cctctttttc aatatatctt ttcctcctga tcgttaaatt cctagatgaa
     3301 ctattcgtat ccaaacaaat ttagcctagc ctttgacaat ttttgctcga ttacctcgca
     3361 agacaatatg actacgtatt tatttttaac agttcttttc agtaaactag ttgttaggtg
     3421 tatcttctaa tgtccaaact aatttagtgg aatattgttg cgctaacttg acagatttac
     3481 cgggtaccga aagctgtacc gcatcttgat tttcattttc atttgcacca aatcgataga
     3541 cccaagtccc cacacctgtt cctgatttag cattaattgc taacgtattg tttcctcccg
     3601 gtgtcaacgt taattcagaa ttcaccgttt gcggcgtata tgttggatca accaaagaaa
     3661 cagcctgccc tttcgtaaat ttcagctgtg ctccaactag ttcatcccca ctggcagttt
     3721 taaactgttc tggttgagag acacttaatt tccacccaga taaggtactt ctcctatctg
     3781 tcacttggac atatgtcggt tttctagacc catcgttcat cacatcagca gctgcataat
     3841 acgttttatc cgcagtagaa attttttgag tcccaaattt gaattttgat ccataatcaa
     3901 tcgataatgc ccctcctgtt ccagggcttg gaggatctga tggatcgaca ggtttattgg
     3961 gatcaggatt ctctggatca acaggaggag ttacaccctc atctcctgct tcaaatgtaa
     4021 tcgttccttc tgtgttatat tcctttgggt atacttcagt tgcagaaaca aaagtcgtac
     4081 ttgctaccat gctgaataac atcattgctg ctgttaatac gatttctttt ttcatcattg
     4141 acattcctct ttctttattt actaaacata tcagtcgatt ggattaatcc atagcaaaat
     4201 tctgttttac gatacttatg aaataaaaga acatagacct atatttggtc ctatgtatcg
     4261 attagttata aatgattatc agcccgattt gatttctagt ttttattgca acttgatgca
     4321 aacacaatct agaaatacag ctgtcatagc ctttaaaata attcgtaagt ccccctccta
     4381 tctatcaaaa tgtgacttga ctgcctagac ttcaatcaat attaattata attaaataaa
     4441 aaatgtatat tatatttttt ttgtgattat cgttcaaaaa aagaatacac gcttgcatct
     4501 agtactctgc gagttacaaa tcatccgatc aaataaacat tcgctgatta ttttccgcaa
     4561 aaaaagcagc atatccacga taaatggatc tactgctctt acttatcctt atgttttaca
     4621 aggtattttc ttttacacat tactaatgct gtctcaatac ggaacgattg tcatcgcttg
     4681 ttcaaattcc tcttgcaaat gttgtgtttg ataggcctga aaagctgggt gatgggcacg
     4741 caagaggaca cgtaatttca taggatcata gctgtctagc aactgttctt tcaagacaaa
     4801 atctcgggtc agctgccatt cataataata accaaaaggt ctgcagctgt aaatcgtgtg
     4861 accgatggat ttatcttcaa agcgacgatg cgtatggcca aaaactactt gtttgatatt
     4921 tgaataacgg tcaagtaatt ctccgaatga ttctgatcct aaaaatgcat ttaatttgtt
     4981 ccaacgctca taaggagcag attgatacat gataaatgcc cgtttaggaa cgaaatgagt
     5041 ggataaaatc acctcaagtt tttttgcttc cagctggtct agcaaatttt ttgcagctgc
     5101 aagcatttct gcattgacct cgagatctgt tttcccacgc tggatgaacc gatcatacca
     5161 atacaaacgc ttcattcgta cgattgcctc gtaatctttc aattcggaaa actgatagtc
     5221 gtaccagcca ttcatcccta aaagaacggt ctgctcattt aaagcaatat accgttcatt
     5281 taaaaaatgt gggtctggaa aatggtccat catctgttca ttgggcacat ctgccaactc
     5341 gtgattgccg aaatgaaatg tcgtagataa tcctttattg acgaaaaatt cgacaacagc
     5401 taggcaacgg tctactttat tagccgtatc tccggctaga tgaagccgag tgatcttgtg
     5461 cttttgcaaa acttgccaaa gctgcatcag ctctgattcg ccgaatttat tgatgtccac
     5521 atgtaggtcg ctgatgatcg ccaattttcc cattttatcc ctctagtctt ttcgaacaac
     5581 ttgcaagttg ccttttctca agccgcttgg tccttttaat tgtaaactat tataagttgg
     5641 ttctaaagca agtgtggatg gatcatccat gtaaagtatt gcactgtctt tgattttgat
     5701 tggtccaact ggtgtatcag ccactacaga ataaagactt agatctgttt gtgtatcttt
     5761 tttgacaatg atcaatcgaa atgccgtatc caattggcaa ctgacttggc tttctgcaaa
     5821 tggaccgtca ccgttttcga tatctaataa aagttgatcg tctgcttcta atttattctt
     5881 caatacttct tgtgcttctt ttgtcacttc taatttcatc tgtatcacct catgtcatag
     5941 aataatcact ttattcatca gatgcaacta atttgtctgg ttctgactgt tctcaattta
     6001 ttggaaagta agtcattcgt ttattatgat ttctcttatc acaaaagaga caactttccc
     6061 ttttcttcgt tcagtttctt gaacggaaaa agacccaaaa agcaacctgt tatttgttta
     6121 accttattcc tttcttaaat aacaaaaaat actataaaaa ataaaaaacc tttaacaact
     6181 attggaaaaa gaattacttt ttgttcacgg attattaaca actcttcggt agagtaaact
     6241 tatcaaataa aagacttctt tggaggaatc actatgaagg aatatataaa aaacatctat
     6301 tttatcgaag aaacacaaaa tatcgagggt tcttatattg aagtaaaaac actctttgtc
     6361 aatgaagata aaaccaaagc attggatatt tataaaaaat tagcttcaaa aaaaacgaat
     6421 agttttggat tgatattaag cgagtataaa atcaaagcag aagagagtta tttctatcaa
     6481 ttattaaaac ggtggagtaa gcttcccgcc gatttttatc gaaaaatgca gatcatcaac
     6541 tatcaaccgc ttgctgagac acatgcctaa atagaggttg tgaaatcagt gctttgagct
     6601 aataccgaag gtcagctgat tgaacattgt ttagaagaga ttgtgacaac tgcgatttgc
     6661 tttgagtaat aagaacgctg gacagaaaat agcttctcat attttctagt ctagtgggct
     6721 tattgccgag aagcagcaga tggaacatcg tttagaagag attgtgacaa ctgcgatttg
     6781 ctttgagtaa taagaacgct ggacagaaaa tagcttctca tattttctag tctagtggac
     6841 ttattgccga gaagcagcag atggaacatc gtttaaagaa gagatcggta caaaataaaa
     6901 atatctaaat ttaagtttta tctaagtttc gctttgtacc tttgattcaa atcaaaggag
     6961 gcgatcttga tgctgaaaaa atggatcaca ctattttttg ccactcccca ttctagaatc
     7021 aacccgtatg atgtagataa tgaattcacg ttgaaaaaag atttctcaaa taaataaaaa
     7081 ccatccgtaa aatcatcaat acgattttac ggatggtttt tgataacttc tttgaggata
     7141 ggctcttgct atttaagaat gtaagaaaca ttattgttac atagtatgag agtttaatcc
     7201 tctgttattt atgctattta aatgcttgat tcacatcttc gatcatcagt ttcattgatt
     7261 ctaaaccgcc cccgcttaaa taccagacat ctggttcaag ggagatgatc tgctgatttt
     7321 taccagcatt cgtttgtgct accagttcat tggcagaaat atcatctttg ctgtcgtctc
     7381 caccaatagc ttttgttctg tctactacaa ataagatatc cggatttttt tccagcacat
     7441 attcataaga aacactttga ccgtgagtgg atgcttcgat ttgatcatct gcttgttcaa
     7501 agccaaataa atcatggatg aaaccgaatc tagaaccaga tccgtaggct gataattgcc
     7561 cttcattcac aagtgtgact agtgcttttt tgtcagtagc ttctgctttc tttttcgtct
     7621 gatcgattgc actagataat tcctctaatt gagttttagc ttcttcttgc ttaccaaaga
     7681 tctcccctaa agcagtgaca ttttgctgga aagattccca tggatttttc gcatctaaag
     7741 aaaggaaaat ggtcggagca atttgactta gatctttttg aaaatctctt tgtcggccag
     7801 aaatgatgat tagatctggc tgaagctgat taattttttc taagtctggt tcttttattc
     7861 cacctgctga ttctactttt ttataggatg aaagatactc aggaaggctg cttgtagctg
     7921 ctccaactac cttatctcca acaccaagag catccatcgt atctaaagaa ccattgtcga
     7981 atacgacgac tttttctgga ttatttggca cctcgatttg gtctccatta ctatctgtta
     8041 tcgtcaatgt agtttcagtt tgtgtgctgc ttttttttgt gtctgcctga ctggtgttgt
     8101 tgctatttcc acatgctgct aaaccaaatg atgctagaat caagatacct actactaatt
     8161 ttttcatctc ttctctccta atgttagttt tagaaataaa gacagaatct gcggccttca
     8221 atctcacaaa tccgtatgtc catttcgtat aattcatcca atacatcttt ttttatcatt
     8281 tcttgggtcg ttccttgcgt aaataactgt ccatctttca ttgcaacaat ttcgtctgca
     8341 taacttgcag caaagttgat atcatgaaga acaataacaa ttgttttccc gatttcttgt
     8401 actaaatttt ttaaaatctg catgatttga accgcaaaat tcatatctag attgttcaaa
     8461 ggttcatcca gtaaaatata atccgtgtct tgcgctagca ccattgcaat atacgcacgt
     8521 tggagttgac cgcctgaaag agtgtcaatc gattgatccg ctatttcttc taacccaagg
     8581 tgttccagtg attcctgaac gatttcctcg tcttttgtcg tcaattttcc tttcgtataa
     8641 ggaaatcgtc caaattctac taattctttc acggtgattt ggacatttat ttcattcgat
     8701 tgtcgtagga tcgataattt tttggctaat tcattggatt tccaagcctt tacttcgtta
     8761 tggtctaaat agatttcacc tgtatctttt ggtatcaaac gactgatcat cgacaacacc
     8821 gttgactttc cagcaccatt tggaccgata aaagcagtca atttcttttc aggaatcgtt
     8881 aactgtacat ctttaaccac tgattttcca tggtacagtt tattgacctt ttgtaaatca
     8941 atcataggat actcttcctt tcgcgaatca attttgtgac aaaataaatt cctccgacaa
     9001 attcaacgac gacactcact gttgtattcc agccgaaaat ctgctctact gcgagctgtc
     9061 ctcctgtcag aaacagcata gctaccagaa agcttccgat aaatagctga ccatgacgat
     9121 aagtagaaaa gacacgataa ctgatatttg cgacaataaa ccctaaaaaa gtaactggac
     9181 ccacaagagc cgttgatgtc cctgtcaaaa gactgacaca acataaaatc aaaaattcaa
     9241 aacgatctgt gtctatcccg aggtttactg cttgttcatt tccgagatgg aaaacgtcta
     9301 gcacatgtcg atatcgaaat aacaggtacc agcagccgat aatgatcaaa gaagcaggca
     9361 aaagatactg actctggata ttgccaaagc ttgcaaataa tctgccttga agcaaatcat
     9421 attcattcgg atccatgatg acttgtaaaa aggtactgac actaccaaaa agcgtaccta
     9481 aaatcatccc tgacatcagt aataaaaaaa gattcgctct catttttttc aaaagaaaga
     9541 aaaataacaa tagactcagc ccgaccatca gcccaacgtt caataagaaa aaaactgtac
     9601 ctgtctgctg aagcatcgtc actcctccga taaagaagaa aaggaccgtt tggacaagga
     9661 catataacga ctcaagtccc aatactgaag gagtcaaaaa ctggttatgc gtaattgtct
     9721 gaaagctgat tgtggcactg ctgacagcta gcgagacaag gataaatgca aaaattttct
     9781 tactcctcaa tgcacatgca aaatgccagt ttccatatgt atggatcgtc aagtacattc
     9841 ctgtaacaaa taaaacaaga acaagcatac caataaaaat agatggcttt tttttaagca
     9901 tatcgtcgtc cccttatcag aagataaagg aagatagccc caccgatgat tccaacgatc
     9961 acactgactg gtatttcata aggaaaaatc aacacgcgag cgacaagatc gcaaaatact
    10021 aagaacaaac tacctgctaa agcagttacg ccaattgttt tgtgcacttg atctccatat
    10081 attctagaga caagattcgg aatgatcact ccgataaaag gaatgcttcc taccatgatc
    10141 aacatgactg cactagccaa tgatactaag ccaatgccta aaaactgcat cttctggtaa
    10201 tctattccca gatttactgc aaacgattcg cccagaccaa tgatcgtaaa tcggtaagca
    10261 aatagccaaa ccaagagcaa caatggaaca acagcataca ataactcata atttcctcgt
    10321 atcacagtcg caaaattccc ttgaagccat gaagtcatat tttgtaccaa ctggaattga
    10381 taagcaaaaa aagtagcaac tgaaccaatg atattcccta acatgactcc aatcaacgga
    10441 agcatcaatt gatttttctg gaggaacaat cgcgtaaggg ataaaaaaag caatgttcct
    10501 gcaaaagcaa agcaaaatgc tgtcaaggat cggacaatca aggatgcacc aggtaggaaa
    10561 atcatagcca ctaaaatacc taaacgcgca ctatccatcg tgcccgcagt cgttggtgaa
    10621 acaaatttat tttgggtcaa atgttgcatg atcaacccac aaatactact ggtcgctccc
    10681 acaaggatca aacaaatcgt tcgtggcaaa cgagtcacta gcaagatttg taagctttgc
    10741 ccatctaact ggagcagttc tgacaattgt acattcgata ctccgatgaa caatgaaata
    10801 ctacttaaaa taactaataa tgctactagt cgtccgtagc gcatccaatc cacctttcta
    10861 attgagaatg attttcattc ttattttaag tgaatcgttg aacaaaagca accgcttttt
    10921 catatgtttt aggtattatt gggaatatgc tataatcgta agaaagacaa agagaagaag
    10981 gagggtattt tttatggcac aagatgatgt aaaaagccat ttggataagg gactttatgg
    11041 tacaccttta gtgaaccctg aagaacagca taaatattta gggacattca gggaacgctg
    11101 ctatcttagt atgactgttg ctgaaatgat ccaaccaaaa aacaaagaaa actttttaaa
    11161 agaactcaaa aaacatccag atgcttccat actcttgaat ggggctctac ccctttctgt
    11221 ccaaaaccaa tatatccagc tagcaacgaa aaacaatagc cgtttcactg tagtcaacga
    11281 ttacgtcact gcaaatcctg aggaattcgg attgctctta acagcaaaag aagcagtaaa
    11341 tgaacctgtg attgatatcg aaaaaaaata cccagaagaa aactcttcgc cagctgaaaa
    11401 agaaaaacca aaaaagaaaa aaagcttctg ggatcgttta ttttaaaacg aaaaaacaca
    11461 taaattttta catgcaaaaa aagcttttga tcagattgac atcaaatagg acaatctgtc
    11521 aaaagctttt tcttttaaaa tacttcagga tataacaatt tcaatacatt catcgtcaat
    11581 cttctgcctt ttcctgcaaa aggataaaca tgcatatgca tggctggatt gaaatttgct
    11641 tcgatgatcc catatgcttt tttatcagtt gtaggatcga tttctttatc tggtataatc
    11701 aaatcgatac cactaatctt agctcctagt gcttctaccg cagaaacggc gatttttttg
    11761 taagcttcag aaaattcatc agtcatatcg atcgaatcac cacctgtact gatatttgag
    11821 ttctccctta gataaacaat ttgattggct tggggaactg attcgatcgt caacccttgt
    11881 tctttcaaca tcaattgttc taattcacct aattggatca attctaaagg tgcacgatga
    11941 ttcgttccac gcaaagggtc actatttttt tcttcaacaa gttctttcac tgttcggata
    12001 ctgtcaccaa taacattagc agggactcgc aacatgatgg cttggacttc tccgtcgatc
    12061 acaaagaaac gatattccgt tcccggcata aattcttcta ctaaaaccga actgtcttca
    12121 cgaaaagcaa tggctaaacc tgcttgataa tcttccagac tagctccttc cttgaaaatc
    12181 gtgataccta acccgtaatt ggttgattta ggtttgatca caaatgcctg ctcggcaaat
    12241 ctcggataag cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc tggtacccgg
    12301 aacccagctt cttttaagac ttttttcgtt actgttttgt tttccatgat aagtgggacg
    12361 atatagctgt ccttacttgt catgtttgca tttttcacat attctacatg gtcttggaat
    12421 tgcagacgca aaaattgatc cgactcatct aatactttga cttggacacc tttttggatg
    12481 gcatcgaaca taaatatctg agtagaaagt tccatttcgc gaaaaccggc caactgatag
    12541 ggacgttcat ggctttcttt ataatattga tgtcccaatg ccacagcaac ttcttgctga
    12601 ctcgatagct gtgcttcttg atagagtttc gctgctaacg tttgttcagg atgatccatt
    12661 tgagtcaatg cttcctctac taaataaaga gaatccaacc aatccaattc aaccagcatt
    12721 tgcttcattc cttcaagaat ttctctacct tcttgatgaa attctgtttg atctgatggt
    12781 tgttctagtg ctactttgtt atttcgagtc gtaccttctg ctacccattg gtcacagtct
    12841 tctttttctt ctgtccataa catgtacatc aggaataatt gtaagaaccg aacttgctct
    12901 ggattgatac ccaatcttgc ataaggattc aaatcgatat tccgcaattc aatataacga
    12961 acacctgttg ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag cggtgtataa
    13021 aattctttct cctccgaaag tttcccttct tcgatcattc gctcgatatc tgctaaatac
    13081 tgtttcatag aagcatagga aactttcaca tttggatgat tcgtatagcc cagcgcactg
    13141 ttgcgaatac tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta gttcttctca
    13201 ctcattggtg aagcaccgaa taaataagtg atcatccagc gatagcgcat aaagtttctc
    13261 gctgttttca aataaagttc tgttttgaat tcggaaaaat cttgaaattc ttcctgatgg
    13321 ctgaacaatg ttcttagcaa ctcatcgcca aattcaaaat tgaaatgaat accgcttacc
    13381 atctgttttc gcttcccgta ttcttttgct aaataacgtc gatacaagac atcttcgaaa
    13441 ttttttaatt ttgcaataat gatctcttcg tctttttccg gtaaggcagg tggcatactt
    13501 aatggccaga tcatctcttc tttcggtata gaacgagcag tcacatcata aacagcagcg
    13561 agatactgaa ataattcggg aatagaatcg gtaacagggg tgatcatctc tgtttgtgtt
    13621 tcgctaaaat ctgtttggat ataggggtga taggatcgat cgccaaagat tgctggatga
    13681 tcggtttttg ctagatttcc tgcaagatca acccgctgtc cttcccgttc tataccaaat
    13741 cgagcttggt cgataaaagg gcgcgcattg acatgcaata ataattgctt aaaattcatc
    13801 attaagtgac ctccaattca tatgaatcta attataatca aaaagtctaa gaaaaagaac
    13861 gaaattgttt gagaaccgct tacaaataag tataaaaatg aaggcatcaa aaacacagct
    13921 atgttaaaaa caatcattca aacaaggagc tgcaactttt gtcacagctc ctgcttttta
    13981 gacatgggtt attcttcttt tagtaatcct ttgaacaatc caacgacaaa atcatttgga
    14041 tcaaacggct ccaagtcatc gattccttca ccaagaccga ccaatttcac tggtaaatgc
    14101 aattcattac ggatagcaag tacgataccg ccctttgctg taccatctaa ttttgtcaaa
    14161 acaagaccgg tcacatctgt tgtttctttg aattgttttg cttgaaccat cgcgttttga
    14221 ccagtggttg catctaatac aagaagtgtt tcttggggtg cttctggtaa ttcccgctgg
    14281 attactcgtt ttattttttc taattcgttc atcaagttca ctttattttg cagtcggcct
    14341 gcagtatcaa ctagtaaaac atctgcctgc tctgtctttg ctctttccaa agcatcgaat
    14401 acgacagctg ctggatcgcc tcctgcattg ccacgaacaa cttcaacacc agcgcgttcg
    14461 ccccagacaa ctaactggtc gattgctcca gcgcggaagg tatctgctgc tgccatcaat
    14521 acttttttac cttcgttttt gaactgatgg gcaagtttac caatacttgt tgttttccct
    14581 acaccattaa caccgacaaa caaaataact gttagtccgt cttcttggat atttagttca
    14641 tttacttcat tgattccttc cgcttcatag agatccacaa gtttctcgat gatcgcattt
    14701 tggacttctg ccgatttttt cgcatttctg agtttcactt cttgacgtaa tgcttcggta
    14761 attcgtatcg ctgtttcaaa acctacatct gcaccgataa gtgtttcttc taattcttca
    14821 aaaaagtctt catcgactgt tcgaaaattg gcaaacaact cattcatccg ttcaccaaat
    14881 gttttacgcg tcttttctaa acccttgtcg tacttctcct gtacttcaac ttcttctgtt
    14941 ttctcttcgc cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg actcccccta
    15001 ctctaataca aatttttgga tcactttggc tacgccattt tcttcattgc tggctgtcac
    15061 gaaatctgct gcttctttga cgaaaggcac agcattctcc atcgctacgc ctagtccagc
    15121 atattggatc atcggtaaat cattttcctc atcaccgatc gccatgattt cttcttgttt
    15181 caaaccaaga tcttttgcca ataaagaaat accataagct tttgtaacac cttttggcat
    15241 aaattccaaa agcatacttc ttgttttgat gatctcatac atttcatgga attctgctgg
    15301 tatttctttg attttaggat ctaattcttc ttggtcataa gcgattactg ccttattaag
    15361 cacaaattca tctgtaatat cgcttatatc cgctggttgg aattgcagca gcttattcaa
    15421 cacattgtac aaggattgct tccttggggc actaggcagc tgtaaaacca cattgtcaga
    15481 caaaacatct agtggcagat ccaattgctg agccagtttg tataagcgtt ggatatctga
    15541 tacttccatc actttctttt ccatgatttc accagtatca tttttttgta ccaaaccgcc
    15601 gttgaaagtg atgctgtagt caccttcatc taccagtcct aattcttgca gataatgcgc
    15661 catcgcagct aaggggcgtc ctgtacatag aacgatcttg accccttgct gttttgcttg
    15721 tgctaatgct tgtttattct catctgaaat cagcttttgt tcatttaata atgtaccgtc
    15781 caaatcgatt gctactaatt tgatcatttt cgttcctaca ctttcgtttc gttgattttc
    15841 gcctcttcat tgatatcttc catccgtaca gaaacgattt ttgatacacc ggattcctgc
    15901 atcgtcactc cgtacagtac atctgctgcg accatcgttc ctttgcggtg tgtgactacg
    15961 ataaattgtg tgtcatcttg gaaatcgctc aagtaacgac caaaacgttt cacgttggct
    16021 tcatctaacg ctgcttctac ctcatctaag atacaaaacg gaacaggaca gacacggata
    16081 atggaaaaga gcaacgcgat ggctgtaagt gctcgttctc ctccagaaag caaacttaga
    16141 ctttgcagtt ttttgccagg aggttgtact tcaatctcaa tacctgtttt cagcaaatca
    16201 gttggttccg tcagtactaa ttcagcttta ccgccgccaa acatatttgg aaagacaatt
    16261 ttgaattctt gacggatagc ttcaaatact tctttgaatc ttgtccgcac ttcatcatcc
    16321 atctcatcca tcgtttcaaa caattggttt ttcgctgcta acagatcatc tcgctgtgtt
    16381 gctaagaatg tatgtctttc actgacttgt tcgtattgtt cgattgaatt taagtttact
    16441 ggtcccagtt tttcaatctg ttcttttaag gatgaaacct ttgttctact gtcttcgata
    16501 tcggttgttt cttgatagtc ttgggaagct ttctcaaatg tcagctgata ttctgactgc
    16561 agataggtca aatgattatc taaaatcatt tcagctcggt ctttttggac ttctaatttg
    16621 gattgttccg acagtcgtgc tttttgctca cgattacgct ctgccaagac tgcttcaagc
    16681 ttatcgatct ccttttgcct tctatctcgc tgctctttgg cttttgccaa ctcggctttt
    16741 aatgtttctc gctgagcaga taattcgtta atttgttttt ccaaactttc ttctgtgact
    16801 tcatgatcag aaaagtctgc tgtcaatgtc gccaactgct tttctatcgc ttcttgacgt
    16861 tcggttgcct ctgctttttg gactcgggca ccgcggagct ggatctgcag atgattaaac
    16921 tgctctttca agacagcaag atctgcttgt tcttgtgctt tttgactttg tacttgtgct
    16981 cttctggctt ccatctggtc gctttcttga cttaacgatt tgatttcttc gtcgattttt
    17041 tgtcgttgcg attcaagatc tgtttgtttg tctttcaatt cgttcgtttg cttttcgtaa
    17101 tcttcgataa actgctgcag ttcacgtgtc tcaaaagttg agatttgctt ttctttttca
    17161 aaacgttcaa gttcatttat gatgttttgt agctgatttg tcgcttcttg ttcttcaaaa
    17221 cgtaattgtt cgcctcgtgt acgcaaaact tcttgttctt cggctaaacg agctgtttct
    17281 tgttgcagct cctgaacttt tttttcttgt gcttggagtt gtttatctgc ttcttcaaat
    17341 tcactagtca actgtttcaa ttcttgattt tgtacaaaca aactgccttg gtttcctcgt
    17401 ttattcgctc cacctgtcat ggatcctccg gcattcatca catcaccttc taaggaaacc
    17461 actcgatatt gatagcggat cgtttgcgcg attgcattgg cactagttaa gtctttggct
    17521 aaaaggatcg ttcctagcag gttttgaacc acagtttgga tttgatcggg ataactgact
    17581 tgttcactgg caatccctat aaacccttct acagctgctg cttgtgtcaa tatatgagcc
    17641 ggtaattgtc ttggtttgat tgtcgtcagt ggaaggaatg ttgctcttcc tcctctttgc
    17701 tgtttcaaat acgtgatggc ttgccgagca tctttttcat tttcaacgat gacatgctgg
    17761 gctgccccac ctaaagctgt ttcgatagct aacgtgaaat ctgcaggaac atcgatcagt
    17821 tcagccactg ctccgacaat acccgaaagc tgctgtttat gctgcaagac caatcgaacc
    17881 ccttggtaaa aaccaaaata gttttcttgg atttcttgaa ggcttttctt acgagcacgt
    17941 agctgctgga cttgattcat taattgatac attttaggct gttcttttgc taatgcttct
    18001 tggaactttt tcccgttttg ttggatcatt tctaacttct cttttgtttc agttaaagat
    18061 gattttagct gcgcctgctt ttgtgtcaat tcttcttttt gtaagatcaa tcggtctact
    18121 gatgcttcta cttccgattg cttggctaac gtctgcttgc tttttgctgt ttcttgtata
    18181 tattgacgtt ctagatactt cagttcgttt cctacagctg cttgttcttg catcaaatca
    18241 acatattgat ctcttaattc tgccaataat tctttcgttg atttttggta tttttctaat
    18301 tcttgctgtg tttttatgag atttgcttca gctttttgga tctctgtttc tttttctgct
    18361 gctgctttca tcaagctttc ttgaagcttt tcaaaatgtt taaccttttt ttgtgcttct
    18421 gctaatgaag tttgatattc ttgcgagctt ttttgtgtat gctttgtccg ctcctgcaaa
    18481 acatcttttt gtccttctgt ctgcttcagt ttttccgaaa gatctaataa tacttgctga
    18541 tttttttcga tcagacgatc ggcttgcgca ttttctttac gctgcttcgc caaaatggat
    18601 tcttgttctt ggatcgactc agatagtttg cctaattcta gattgaattt cgcaagctgt
    18661 gcttgtttgt tgtcccaatc tttttttgct gtcttgattt cagcaaccat caaagagaca
    18721 tcggtttgag tcagtgtttc ttttagccgt aaaaactcct ttgctgcctc actctgggcg
    18781 gccaaaggag ttaactgttc ttccaattcg tggatgatgt cttggacgcg gctgaggttg
    18841 tcttctgttt caaaaagctt ctgttctgct ttctttttcc gctgcttgta tttcaagaca
    18901 ccagctgctt cttcaaagat cccacgacgg tcttccggtt tactactgaa aatcgcttct
    18961 acttttcctt gagaaatgat tgagaacgat tcttttccta atccagaatc taagaacaat
    19021 tcttggatat cttttaatct gcagctttgt ttattgatga aaaactcgct ttccccagtc
    19081 cgtcgataac ggcgggttac actgatctca ttgtactcca atggcagata atgatcacta
    19141 ttgtctaaaa tcactgtgac ttctgcaata ttcagctgct tacgggtatc ggaaccggca
    19201 aagatgatat ccggcatttt tccgcctcgc agacttttag cagattgttc tcctaatacc
    19261 cagcggatag cctctgtaat attgcttttt ccgctgccat ttggtccaac cactgctgtg
    19321 acgctatttt caaaatcaat gactgtcttg tctgcaaatg atttaaaacc ggctatttca
    19381 attcgtttca aatacaaagc tttccctcac ttacttttac agtgctgcca atgcattttc
    19441 cgccgcagct tgttctgcta attttttcga tttcccttgt ccagtgccaa tcaattgatc
    19501 atctacataa acttcgatcc aaaatactcg ttcatgagct ggaccttctt cattgatcaa
    19561 tcggtattcg attgatacat cacctgattt ttgcaagact tcttgtagtt ttgttttatg
    19621 atccatctca tgtgaaaaag cacctgcttt gactttagga aaaatcactt tttcgatgaa
    19681 tgcatgggtc gtgtcgaatc cctggtcaag atataacgcg cctaaaaaag cttcgaatag
    19741 gtcgcaaagc aatgctggtc gcgtacgtcc tcctgaattc tcttctccct tgcccaacaa
    19801 aacatattga tcaaagtgac attctttggc aaatttcgac aagctgtctt cgcgaacgat
    19861 cgcagctcgt gtttttgtca attttccttc tggcaaatga ggatataaac ggaataaata
    19921 ctgagaaacc atcagttcta aaacggcatc tcctaaaaat tcaagtcgct cattatccga
    19981 taattgcaga ttgcgatgct cattcacata ggatgaatgg gtaaaagctt gttcaagcaa
    20041 atttagatcg tgaaaaacga tgttatagtt ttcttttagt tcttttgtca gctggttgtc
    20101 cattttccac attcctttta tggatatttt ttcgatactt ccttattata ctggtttttt
    20161 gtcaaatttg aaatggttcg gtcaaaaggt aagtaaagct ttaataatcc tagtgattga
    20221 cgagaagttg tgaaaaattc gtttagtttc gagtatgaag aaaaaatttt gtgaaaatgg
    20281 ctttccatat ttttgcataa atttgactta ataccgagaa actggacttt gaacaccgtt
    20341 tattggagaa gtcatgaaat cagcgccttg acctgaatat tagagaaata aacagaaaaa
    20401 tatcaaaaaa ccgaaaaagg acatcgccct ttttcggtca tttttagtga ttatgaacgt
    20461 gaatctgctg taacagaaac atttgcccat gggtttggta catcataaga ccaatcccag
    20521 tctttgacac ggtcgttgac tggcaatact tcattacgga acaacgttgg aatcgcaaat
    20581 gcttcttcaa atgcatattg ttgccatttt tgatatgctt ctttttggta ctcttcatca
    20641 aatgctttgc ttgaactgat atcgttgatc aattttgtat tttcttctga ttcataacga
    20701 gtgtagttga acgcagcatt tggtccccat agtccacttg gatttggatc agttcctgta
    20761 ttccaagcag cttggtaaac atcaatttca ggatcatcgt tttctaattt atcatagaaa
    20821 gaattgaatt cgatcagacg acctgttgtg tattggacat ccaaaccaat ttctttccat
    20881 tgttggatat agtaatctgc caatggttgc gctgtttccc caccggacat cgaagcaaag
    20941 ttgattgtta gtttatttcc atctttgtct tctcttaaac catcgtcatc cacatctttg
    21001 tagccagcat catctaatag ttttttcgct ttgtctagat cttgtgtgaa tcctttcatc
    21061 tcatcatcat ggtatgactg gaatactgga ggaatcaatg aagtcgcatt gcttctcaaa
    21121 cctgcataga aacgttcacc tactgcatta ttatctaatg catagcccat tgcttgacgt
    21181 aatgatttat cagccatttt tgaatctgga ttgtattcca cactctttgt atctgcattc
    21241 catttaccta acttgaatcc tagataagta taagaagttt gttcacggcc taagtttgta
    21301 tagccagcaa tttctttata ggtatcgtaa gtatctgttg gcatttggaa aatcatatca
    21361 tataatttag agttcaatgc ttcagttgca cttgccatcg gtacagattt catcgtgatc
    21421 tttttcaatt taggcgtacc attccagtag tgctcgtttg gcaagtattc tactgattct
    21481 cctgcaacga tattactcat gtaataaggg ccaaaagtca ctggattttt acgtacttga
    21541 tcgcttgatt ccatatcttt gattgctaca ccttctaacg tatgttttgg tgctgcatat
    21601 gaccagatac caccacccga ttgaagcatg cttgggttta cttctttata ttcgatttca
    21661 actgttttgt cgtctatttt tttgattccg gagattgtgt cggcacttcc atcatgatat
    21721 tcttccatac ctacgatgtt acggaaagtg tcgtcataac ggataccagt gtaatcttta
    21781 ttcccaatga tttcgtaagg gaaaatcaca tcgtccgccg ttacatcttc accatctgac
    21841 catttgacgt tgtcttttac tttgattgtc gcttttttcg tatcttcatc tagatcaagc
    21901 gttgcagctc cgtcgtctgt gattctgaaa tcgtcatcgt ttgcaaatag cggttcatga
    21961 gaaggagcca taaaagctgc atcatatgaa tcttggtaaa actcccactg gaacaatcct
    22021 ttaaactgac tgtccgttgc gaccgcagct tctaattcgc cgtcttcgat tgcatcatca
    22081 ttattttttg ttgcaattgg aaaagttaat tcctctttgt tattgccgtt gcctccagag
    22141 ttgtttccag aggtatctgt acctccgccc ccgcatgctg caagaacgac agcagacatc
    22201 agcgctgcaa atccaaaaat cgttttcttc attcctattt cccccttcgc ttctatacta
    22261 gcctaaccgt tggcgcgcat ctgcagaacg cttgaatgct tgtccaacat aattgatact
    22321 caacatcaaa actaaaataa ggattgatgc gggtaaccag atccattgcc gattcactaa
    22381 gacttctccg ttacttgcat aaccgatcaa tgtacctaaa ctaggtacat ttgccggcaa
    22441 gccgaaacct aagaaagtca aagttgtctc gataccgatg tttgccgcaa agttcatcgt
    22501 caaattagta atgattaatg aacttagatt cggcatgatt tcgcggaaca tgattttaaa
    22561 atcattggtt cccattgttt ttgaggcact gacataatct cttcttgctt cagataatgt
    22621 cttgcttcgg aataatcggg ctttagctac ccaataaaat gcactcatga tgaatataaa
    22681 ggaccagaca ttgtatctgg aaataatcga tacgaagacg ataatgatca gcatgatcgg
    22741 tagaatcatg ataaaatcaa cgatccgcat caagatgttg tcaatgatcc ctccgtagta
    22801 gccagagatg atccctaatc caaccccgat gatcgaagta aggatcgtga tcgcaaatcc
    22861 gatcgtaaca gagtttctag cacctatgat caattgtcct aatacatcac gtccgccttc
    22921 atctgctccc agaatgaact tttgtccttg tgtcgaaact gttcctggtg cagcgtattt
    22981 gtcgaagata ctaacataca tcactttgtc ttgatcggtc agcatcgcac cgataaagat
    23041 cactaataat aaaaccacta gcaaacctaa tgaaaaaata gcaattttat cttttttaaa
    23101 ttctcgagca atcattcgta tccccatagg aggaatactt tcttgaactg ctgtattgtt
    23161 tttttcactc atcgatccat tctcctttct gattggtttt attgaatccg aatacgcggg
    23221 tcaacgatac tcatgatgat atctgaaagt aatgtcccca ctagcgtcgc gattcctaaa
    23281 atcaatacta aggatgtgat cacggtatag tcccgttggc cgatggaatc aataaatagt
    23341 tttccgactc ccggataacc aaaaatcttt tcgataacta cagaaccagc aatcaacgaa
    23401 gtgatttcat accccatttg agaagccatc ggcaacgctg catttcggaa aatatgtcga
    23461 ctgaatactt tattcgtcgg tacccctttg gaacgggctg ttctgacaaa atcgagcgat
    23521 ttcgaatcga ttacttcact tcgcagatat tggattgtga cggcagtcgc caaaaatgct
    23581 tgggtaattg atggtaacag aatatatttc aaacggtcta tccattgagt gataaatcca
    23641 ccttgcaaac ctagtgtttg tgatccactt gttgggaacc agcctaaacg ataaccaaaa
    23701 acaaatagca tgatcaagcc gaagataaat aaaggaacag caaaactaaa gaagttgtag
    23761 attacgacaa tcttatccaa gattgaattt tgataccggc cagcaagcat ccccagaggc
    23821 acagcaatca aataagtaag gacaactgtc aagatcgata gatataaagt gttgatgatc
    23881 cgttcgccga tcaagtcagc aacagagcgt ttgtataaga aactttgacc aaaatcgcct
    23941 tgcaaagcat ttccgatcca acggaagtac tggacatacc atgggtcatt caagccagct
    24001 gcttgtcgca tctgttcgat cactgccgga tcctgatttg gattgatcaa accggtaaat
    24061 ggatcccccg gcatcgcttg ggctaataag aagatcagaa cacttaaaat aataacttgc
    24121 gggatcatca ataaaacacg tcgcagaatc gttttccaca ttagcttgca cctccatctt
    24181 tcaacgccac tttgtgtgta gttgtgatcg tacgcaaatc gtagactcga ccagtcgcat
    24241 catagtattc tttttggttt tggatatact cttgttctac tcttcgtcga ttttctttgt
    24301 ggctttccct attttctaca tcgatttgcg gaatagctga aagcagacgt ttcgtataga
    24361 tatgtcgcgg gtcattataa atgtcttctc ttgtaccgat ttctacaaat cgacctttgt
    24421 acataattgc gatattgtcg cacatgtgtt ttactacacc taaatcatgt gaaatgaata
    24481 aatagctgag gccgtattct tgctggattc gtttcatgaa attcaatact tgcgcttgaa
    24541 cagatagatc cagagcagat acaggttcat cggctacgat cagttttgga ttggtagcaa
    24601 cagcacgggc tactcctaga cgttgacgtt gtccgcctga aaattcatga ggatatttat
    24661 atagcgcatc ttccggcatc ccaacgatat ctagcagtcc ttttaccttt ttcttttctt
    24721 cttgatcgct taaccgttca aaatttcgga tgggttcagc aatgatatcc aacactcgct
    24781 ttttaggatt caaactggac attgaatctt ggaagatcat ctgcacatcc ttgttataac
    24841 ccattttttt gcggttgctt cgcttcgtta catcttttcc ttcatacatg atttgtccgg
    24901 ctgttacctt ttctaatccc acaacggctt ttcccgtagt agattttcca gagccagact
    24961 ctcccactaa accatatgtc tttccttttt cgataataaa atccacacca tctacagcat
    25021 atacatgatc cgttactcga ttgaagaatc cactgcgaat agggtaatga actttcaggt
    25081 ctttgattgt gattaattct gtcatctaca cctctccttt ttcgtctcta aagtggaaat
    25141 gcttatagca ggagcaacgt accaagtggt tcggtgcgac ttcatgaagg ataggctctt
    25201 cttcatgatc gcttgctggt atccatggaa tacgtggtgc aaaccgacaa cctttacgag
    25261 gcatgttttt cagagaagga acgacacctt cgatcacatg gagttctgca tcatgatttc
    25321 cttcttgtgg tatagagttc agtaaagagc gtgtataagg atgcttagga tgatcaaaca
    25381 gttcttttac acctgctact tcaacgaact gtccgccata catcactgcc actcgatcag
    25441 ccatttctgc tacgactcct aagtcatgag tgatcaagat gatccctgtt tttgtttctt
    25501 cctgaaggtc tttcagtaaa tcaagaatct gtgcttggat ggttacatct agggctgtcg
    25561 tcggttcatc tgcaatgacg attggaggtt tgcaggcgat tgcgattgcg atgatgacac
    25621 gttgtctcat accgcctgat agttcatggg gatattggcg tccaactctc gcaggattag
    25681 gaatccctac ttgttctagc aattctagtg cacgagcttg acgctgttct gcattcatct
    25741 ttgtgtgata ggaaagtcct tctttgatct ggtcttcgat tctcatgaga ggatttaatg
    25801 cagacaacgg atcttggaaa atcattccga tatcatttcc gcggatttta ttatagagcg
    25861 tttcgttcag gtcgattaaa ttcagatcgt tatataaaat ctcacctgta atttttgtgt
    25921 tattaggatc aagtaatccc ataatcgtag tagccaaggt acttttcccg cacccagatt
    25981 ccccaacgat tgctaaaatc tcgtttttgt ctaaagtaaa tgaaacgtca tctactgcat
    26041 cgtaatagtc atctttgagt cggaatcctg tatgtaggtt ctgaacttct aataattggt
    26101 tatcggacaa tcgaataccc ctctctgctt cctaaatgga agtttttctt aataaaaaag
    26161 gacattacac agaataaatc gttttataac aaaggttatt cttatctttg tgtaatgaat
    26221 tattaataat tatatagaaa accgctttac tttgcaacat aatttctgtt tatcgccaga
    26281 attatcagaa aaatgacttt tacgtgtcaa aaagattaaa aaaagaagga taaaaagctg
    26341 catgcagctt tttatccttc tttgctttta tttgaaatta tttaagatgg ctgctgatat
    26401 aatctactgc gccaccgact gtttcaattt gttcagcgtc ttcatctgaa atttccgtac
    26461 cgaattcatc ttcaagctct aaaacgaatt ccataatact aattgaatca gcttttaaat
    26521 catctttgat gttcaattca tccgtcactt tatcggtatc tatctcaaaa tgatttgaaa
    26581 taattttcgc tactttgtta aatacttctt cgcgagtcaa ctttttcacc tccaagaatc
    26641 atacacttgt caattaccta caccatggct aattttactg gcatttttca ggaatgtcca
    26701 cctttttttc aatggaacgg atcgcttcag atatccaaat actattcttc gcttttttca
    26761 aattgttcga ctaattgtcc aactacatct gtctcaagca ttgtgtggat ttggcggatc
    26821 gtatagcgaa cagcatcggg gcctgttgct ccatgagttt taatgactgg tgctttcaag
    26881 ccaaacaaaa cggcaccgcc atgtttggaa taatccattt ctgctttcaa agaacgtaag
    26941 ccgtctttta aaagcatagc gcccattttt cctttgatcc cttcatttag aatagcagat
    27001 tttaatagat tcatcatatt cattgctgtt ccttcaatag atttcaaaac agcgttgcct
    27061 gtaaatccat ctgtgaccac tacatcagcc acaccattga gtagctcacg agcttctacg
    27121 tttccaataa aattcaagct tgtttcattc tgaaggagtt cataggcttt tttcgtcact
    27181 tcactacctt ttgttgcttc tgttccgttg tttaagagtg cgacacgagg acgtccgata
    27241 cctcggactt tacgtgcata aaaagaacct aaaataccgt attgcaacaa atgttctggt
    27301 ttgttttctg cgttagcgcc aagatccagc atatcgaaac cggcaccttc tttccctact
    27361 actggcaagg tagacatcaa gccagggcgt tcgatatttt tgatacgtcc tacgatgaac
    27421 aaccctgctg ctagtagcgc gcctgtattc cctgctgaaa atacagcatc tgcttcaccg
    27481 tttttcactg cttgtgcagc aaggaccatt gatgcagtct tttttcgtcg aattgctttt
    27541 actggctcat catcactatt gattttttcg tctgtatgga taatcgtgat gtttttttca
    27601 tttgttaagt atttcttgat ttctgcttct tttccgtaaa gtaagaattc tacttctgga
    27661 aaatcttgct gcgctaaagc cacgccttct acaattgctt gaggtgcgtg atctccgccc
    27721 attgcatcta cggcaatttt cattgtttcc actccttttt ccgtcatcag tataaagtat
    27781 agcacagtct gctaatcgaa ataacgatta tcctgatttt ctatttggag gctttgcgct
    27841 aaaggctgat actcaggaag catctgccaa tcctttattt gccaaagggc tgcagcctct
    27901 tctctggcta cctccaaaac atttccatcc gtcaccaagt cagcaatcgc aaattgaggc
    27961 agaccagatt gacggaagcc aaatacttca ccaggaccac gtaattccag atctttttcg
    28021 cttagtacaa aaccattggt ggtctctgtc atgattttca tccgttccac acctagttca
    28081 tttttaggat tagcaactaa gatacagtag gaagcatcac ttccgcgtcc aacacgtcca
    28141 cgaagttggt gcagctgtgc caatccaaat cgatccgcat ccatgatcag catgacggtc
    28201 gcgttcggca cgtttacccc tacttcaatt acagtagtcg aaactaatat ctgcatctgg
    28261 ttgtctttga aggcttccat gattacttct ttttcctgat tcttcatttt accatgtaac
    28321 aagccaactt catattttgg tgcaaatagt tcacgaagtt tttcatagat ttccactgca
    28381 ttttttacat ctaatgcttc tgattcttca atcagcggac agatgacata catttgatgt
    28441 cctcttgcca gttctttttt cgtccaatcc agtacactgt ccaattgagg tgttcttacc
    28501 cagcgtgttt cgatcgggat acgtccagct ggaagttcat caatgataga tacatccatc
    28561 tcaccatatg ctgtaatagc taatgttcta ggaatcggag tcgctgtcat aaataagaca
    28621 tctggtcgcc agcctttttc acgaagtact ttccgctggt tcacgccaaa acggtgctgc
    28681 tcatccgtaa taactagacc taaattctga aattcgactc catcttggat caatgcatgt
    28741 gtgccgatca cgacgttaat ttccccttgc tctagttttt caaggagctc acgtcgttct
    28801 tttgctttcg ttgaccccgt aatcaatgcg atggttactt cattgggatc aaacagctgt
    28861 tgcaagcttt ccatatgttg ttgtgccaag atttctgtag gcaccatcaa cgcaccttgg
    28921 aaacctgctg taacggtagc gtataacgca atcgcggcaa ccactgtttt tccgctacct
    28981 acatctccct gcaaaagtcg ctgcatatgc tgcggactgc gaagatcccg acagatttca
    29041 ttcgttacac gtttttgtgc atttgtcagt tcaaaaggta gcttttgagt aaattgtttc
    29101 aatttttgga catcataaag gatctccaaa ccattcgttt ctgctttttc tgcttttttc
    29161 aatccttgaa gacgcatttg aaataaaaag aactcttcaa aaacgacacg tcgtttggct
    29221 tgatggtttt cttcgtggtc ttttggaaaa tgcatacttc ttacagcaga agctcggtca
    29281 agcagtcgat atttctccac aaggttgttt ggcagatttt cttcaatcaa tgtgccgtat
    29341 tcttgaaacc cttttttgat tagatcaact aatgtcgttt gacgaattga tttgttgacg
    29401 tgatatatcg gcgaaaagtc atccattgtt tgagaaccta aaatcttcat cccgttcaag
    29461 gctttacgct ttgcatccca tttaccgtat acggcgattt cctccgataa aatgactttt
    29521 tcttttagat aaggttgatt aaaaaaagaa acgttgaaca catcatgttc ttgcatcata
    29581 cgaaattgca atcggctttt tttataacca aatcgattca tcacaggagg cgagactacg
    29641 attcctttca aagtaacctt ttcttgatcc tggatctcgt ttagatttct ttcctgaata
    29701 tcttcgtagc gaaaaggata ataagtcagt aagtcttcaa tcgtttggat gcccaagctg
    29761 gcaagactag ctgctctttt ttctccgact cctgccagca tagtgattgg atcatctaaa
    29821 gaacgcataa atttttcctc cctcctttaa caaaaaaagg acttggtcag cgatcatacc
    29881 tgatcactaa ccgaaggcct tttatgaatg aatactgtgt tagaaagata ttctgacaca
    29941 gaaattattc agcagaaaat agataaggat agacaggctg atctccttgg tggatctcga
    30001 tttccaaatc aggatgtttt tctgttagtt tctcagcaaa ttcatttgct tgttcaactg
    30061 ttccttcatc acctgccaag atcgttacga tctctgtgtc ctcattgatc atttgttcta
    30121 gtgtacttaa cgctgttgct gtgatatctg ggttagagat caggattttt ccatcgatca
    30181 tgcccaaaaa gtctccttct ttgatctcta caccgtcaat tgaggtatct ctgatcgcgt
    30241 gagtgacttg accgctcaca acgctagaca acatttctgt catcgtttct ttgttttctt
    30301 ctaaagaagc ttgatcattg aatgaaagca tagctgtcaa tccttgggaa atcgttcttg
    30361 aaggaacaac tgctactggg atatctgcta cttctgctgc ttgatcagct gccatgaaga
    30421 tgttcttatt gtttggcaag acaatcactt tatctgcgtt tacttcttga atcgctttaa
    30481 caatgtcttc cgtacttgga ttcattgttt gtccaccact gataacataa ttagcaccta
    30541 aactttcaaa cagctcttgg acgcctttac cagcagcgat cgctacgata ccatacggga
    30601 tacgtggtat ttcttttttc gcaaactcgt tcacttcttc atcgtgttcc aaaatcgtct
    30661 catgctgcaa gcgcatgttg tcgactttga ttttgactaa tgaaccgaat ttttgtccat
    30721 aattcaagac ttctcctggg ttttctgtat gcacgtggac tttgatgatc tcgtcatcat
    30781 tgaccacaag taaagaatct cctataccat ctaaatagtt acggaattct tcgtaatcga
    30841 atgtactatc gactgtaggt ccttctccga tttttaccat gatttctgta cagtaaccaa
    30901 aatggatatc ttctgtcgcc atttgacctt ggacactgcg atgatgttct gcattgacca
    30961 tatcatccat ttctgctgga gaaggttcat atgtttcatc cgcttggaat tctccattca
    31021 atgcacttaa aaagccctca taaacaaaca gcagtccttg ccctccactg tctacgacac
    31081 cgacttcttt taggactgga agcaagtctg gagttttagc taaagcacgc tttgctcctt
    31141 ttacaacagc tgtcattact tcaacgacat catcagtact ttttgctttc ttttctccag
    31201 ctttcgctgc ttctctagct actgtcaaaa tcgttccttc tactggtttc attactgctt
    31261 tatacgcagt ttcaactcca tgagtgaagg ctgcagcaag atcagaagca tttaatgaga
    31321 caacatctgg aatttgctta gaaaatccac gaaacaattg agacaaaata acgccggaat
    31381 ttccgcgcgc acccatcagc aatccttttg aaagcattgc tgctaattct cctacttttt
    31441 ctgaggttga atctactact gccttagctc cactagtcat agatagattc atatttgttc
    31501 ccgtatcgcc atctggcaca gggaatacat ttaatgaatt gacgtattct gcatttactt
    31561 gcagacgatt cgcacctgcc tggaccattt cctggaactg gcttgcactt atttctgtta
    31621 cactcaccta gataatcctc cttcagatcg actgctgagt taccagccag tctcctgcct
    31681 agtctggcag tacacgaaca ccttgtacaa agacattaac tgaattggca gttacgccta
    31741 ataatgcctc tagattgtat ttcacttttt cttggacgtt gcgggaaact tcagagattt
    31801 tcgtaccata gctgataatc gtataaacat ctacagcaat accattatct tcttggcgga
    31861 caactactcc tcttgagtaa ttttctttac gcaaaatctc agtaatatta tctttaattt
    31921 gatttttgct ggccattcct acaacaccga acacatctgt cgcggcgcca ccaacaactg
    31981 ttgcaattac atcattggta atttcaatgg ttccagctga cgtttgaatt tttacagcca
    32041 tgaatgagcc tccttaacgg gtaaaaaact cccatttttc ttctatcata ttttatcata
    32101 gcgcaacttg aaataaaagg cagttaccca aagtactcaa aaaccttgca aaatttcagg
    32161 tataaccttg tttaatttac aattattctt ctcaattgtc aattgtatct atgaaaatgt
    32221 acttgcaaat tcccattcta tatgatatgt tagtctggta tgagctgaat tattctattg
    32281 ctccaaatca aggcaaagga ggaaattacc atggcaaaag tttgttactt tacaggtcgc
    32341 aagacaaaaa gtggaaacaa ccgttcacac gctatgaact caacaaaacg tactgttaaa
    32401 cctaacttac aaaaagttcg cgttatggtc gacggaaaac ctaaaaaagt ttgggtgtca
    32461 actcgtgctt tgaaatcagg aaaagttgaa cgcgtttaat tgaatgatac ttggaccgtt
    32521 cagatagtga acggtttttt atttaataaa aatttttaag ggtgggactt ttgattccta
    32581 cccttttt
//
LOCUS       DAJSMI010000012        31976 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJSMI010000012 DAJSMI010000000
VERSION     DAJSMI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.012"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            125..1102
                     /gene="guaC"
                     /locus_tag="ON430_000488"
     CDS             125..1102
                     /gene="guaC"
                     /locus_tag="ON430_000488"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR3218434.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            1366..2790
                     /locus_tag="ON430_000489"
     CDS             1366..2790
                     /locus_tag="ON430_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR3218435.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            3251..4360
                     /gene="pdhA"
                     /locus_tag="ON430_000490"
     CDS             3251..4360
                     /gene="pdhA"
                     /locus_tag="ON430_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCR3218436.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            4363..5340
                     /locus_tag="ON430_000491"
     CDS             4363..5340
                     /locus_tag="ON430_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCR3218437.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            5365..6660
                     /locus_tag="ON430_000492"
     CDS             5365..6660
                     /locus_tag="ON430_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCR3218438.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            6668..8074
                     /gene="lpdA"
                     /locus_tag="ON430_000493"
     CDS             6668..8074
                     /gene="lpdA"
                     /locus_tag="ON430_000493"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR3218439.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            8236..8511
                     /locus_tag="ON430_000494"
     CDS             8236..8511
                     /locus_tag="ON430_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCR3218440.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            8517..9284
                     /locus_tag="ON430_000495"
     CDS             8517..9284
                     /locus_tag="ON430_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCR3218441.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            9452..11281
                     /gene="typA"
                     /locus_tag="ON430_000496"
     CDS             9452..11281
                     /gene="typA"
                     /locus_tag="ON430_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCR3218442.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            11542..13314
                     /gene="recQ"
                     /locus_tag="ON430_000497"
     CDS             11542..13314
                     /gene="recQ"
                     /locus_tag="ON430_000497"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCR3218443.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            13428..13730
                     /locus_tag="ON430_000498"
     CDS             13428..13730
                     /locus_tag="ON430_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCR3218444.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            13733..14896
                     /locus_tag="ON430_000499"
     CDS             13733..14896
                     /locus_tag="ON430_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3218445.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            14922..18350
                     /locus_tag="ON430_000500"
     CDS             14922..18350
                     /locus_tag="ON430_000500"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCR3218446.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            18371..19495
                     /locus_tag="ON430_000501"
     CDS             18371..19495
                     /locus_tag="ON430_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218447.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEIMSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            19485..19922
                     /locus_tag="ON430_000502"
     CDS             19485..19922
                     /locus_tag="ON430_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3218448.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            19906..20244
                     /locus_tag="ON430_000503"
     CDS             19906..20244
                     /locus_tag="ON430_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCR3218449.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            20363..20923
                     /gene="rsmD"
                     /locus_tag="ON430_000504"
     CDS             20363..20923
                     /gene="rsmD"
                     /locus_tag="ON430_000504"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCR3218450.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            20920..21411
                     /gene="coaD"
                     /locus_tag="ON430_000505"
     CDS             20920..21411
                     /gene="coaD"
                     /locus_tag="ON430_000505"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR3218451.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            21401..22444
                     /locus_tag="ON430_000506"
     CDS             21401..22444
                     /locus_tag="ON430_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCR3218452.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            22603..23331
                     /locus_tag="ON430_000507"
     CDS             22603..23331
                     /locus_tag="ON430_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR3218453.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            23447..23692
                     /locus_tag="ON430_000508"
     CDS             23447..23692
                     /locus_tag="ON430_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3218454.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            23689..23907
                     /locus_tag="ON430_000509"
     CDS             23689..23907
                     /locus_tag="ON430_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR3218455.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            24043..24582
                     /locus_tag="ON430_000510"
     CDS             24043..24582
                     /locus_tag="ON430_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCR3218456.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            24575..26848
                     /locus_tag="ON430_000511"
     CDS             24575..26848
                     /locus_tag="ON430_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCR3218457.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            26886..27932
                     /gene="holA"
                     /locus_tag="ON430_000512"
     CDS             26886..27932
                     /gene="holA"
                     /locus_tag="ON430_000512"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR3218458.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAVQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(28021..28275)
                     /locus_tag="ON430_000513"
     CDS             complement(28021..28275)
                     /locus_tag="ON430_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR3218459.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(28675..28944)
                     /locus_tag="ON430_000514"
     CDS             complement(28675..28944)
                     /locus_tag="ON430_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218460.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(29414..29869)
                     /locus_tag="ON430_000515"
     CDS             complement(29414..29869)
                     /locus_tag="ON430_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR3218461.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(30007..31935)
                     /locus_tag="ON430_000516"
     CDS             complement(30007..31935)
                     /locus_tag="ON430_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR3218462.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        10672 a         5539 c         6775 g         8990 t
ORIGIN      
        1 attctctttt tgctcttgat ttttttgctt aattgagtta aattacatag ggcgctaaaa
       61 aaagccttga ttaaattaat aatgttcgtg ttttgctgga tgtgaacatc aggaggataa
      121 tgtaatgaaa gtatttgatt acgaagatat tcaattaatt cctaataaat gtatcgtaaa
      181 tagccgttct gaatgtgata caacggtcac attaggtaaa catacattca aaatgccagt
      241 agtgcctgcc aacatgcaga cgattattga tgaaacaatc gcagaattct tagcggaaaa
      301 tggttatttc tatatcatgc atcggtttga tgaagcagct cgaattccgt ttatcaaaaa
      361 aatgaaaaag cgtggattga ttacctcgat cagtgttggc gtcaagaaag aggaatattc
      421 ctttatagag aagttggcag aagaatcgct aaatcctgat tatatcacta tcgacatcgc
      481 gcatggacat gctaactcag ttattgatat gattcagcat attaaaaaat atttgcctga
      541 aacttttgtg attgccggta atgttggtac accggaagcc gtccgtgaat tggaaaatgc
      601 gggcgcagat gcaacaaaag tcgggattgg tccaggaaaa gtatgtatta caaaaatcaa
      661 gacaggcttt gggactggag gctggcaact ggcagctctt agatggtgtg cgaaagcagc
      721 tcgcaagccg atcatcgctg acggtgggat cagaacacat ggcgatattg caaaatctgt
      781 ccgttttggt gcgacaatgg tcatgattgg ttcattgttt gccggtcacg aggaatctcc
      841 tggcgaaaca aaagtcgaaa atggaatcgt gtataaagaa tactttggtt ctgcttcaga
      901 attccaaaaa ggcgaaaaac gaaatgttga aggaaagaaa atctggatcc aacacaaagg
      961 atctttgaaa gatacactag tggaaatgca acaagacctt caatcatcta tttcttatgc
     1021 tggaggaaga gatctggaag ccattcgaaa agttgactat gtcatcgtga aaaattcgat
     1081 ttttaatgga gacacaatct aaaaaataaa taaaatatta ggatgttgtg aaaaagttgt
     1141 cctgcattaa gtaataagaa cagccaaaca aaaatagttc tttatatgtt ttgtttggct
     1201 ggaattatta tcgcagatac aagcttttga acatcattat tcagaataag ggactgtgat
     1261 aagatttttg tcacagtccc ttatgttttg caaaaaaagt ttttcttttt tctcgtaaat
     1321 tcggtaaaat caaaagaaaa aatagacaat agaaaagaga agcttatgtg ggaaaaagta
     1381 gagaaaaaac aaggaccgat ttatcgtcaa attatggaac agattatgag agaaatcgaa
     1441 aacggaagga tcaatcctgg aagtcaactt gaatcagaac gaaaactagg gcaacttttt
     1501 ggtgtgaatc gttccaccgt tgtccatgca ttggaagagc ttagggagct tggggtacta
     1561 acgagcaaac gtggcagcgg atggtttgtt aatcagactg aatgggggaa attcgctgtt
     1621 ccaagaatcg attggcggca aatgatctct cctcgttatg agcagacgga ctggtatgag
     1681 caaaaaataa gagaagcaaa gcagatccaa aaaacatcgt ttcttgattt atacgctagc
     1741 gagatgccag atgagctttt gccgaatttt gagtttcctt cttattctct tgaagaaatc
     1801 ttagcggaag aaaaagaaat ggatctttta ggctatactc cgttacgtaa caaaatcaaa
     1861 cggtatttgg aaagtaaatt tgaattggca ttttccccca accagcttct tgtgactggt
     1921 ggcggtcagc aagcaatctt tttgatttta caaacgattc tttcagctgg agaggggata
     1981 gctgtagagt caccttcttt tttttaccga ttacctcttt tcaaagcttc aggaacgcgt
     2041 ttatttggga taccgatgga tgaagaaggg atcgacttag aaaaactgaa agaatcgatc
     2101 cataaaaata aattgaaagc agtgcttatc aacccgaatt ttcaaaaccc tacaggaaaa
     2161 gtgatgagtc aaaaacgacg agaaaagcta gtagaattgt gtcgacagta tcggcttcca
     2221 attattgaag acgatgtatt tacggatctt tcatttctaa accaagaaga agtccttcct
     2281 atacgaacga ttgatccgga taatgtgtta tacgtcggtt cgttatctag ggttttagga
     2341 aaaacaacga aaatcggttg ggtgatagga cctgagactt ttatctcgca actagctcaa
     2401 gcgcaagaaa tgatggagtt ttcgatgaat atccttacac aaatcacagt ggctaatgta
     2461 tttaatgaag caattgattt gaaaatgcaa gatgtaagaa aaaagttgaa agaaaaaagc
     2521 cgactgataa gcgcttgggg agattcacaa tcgtttttca agcttcattc aattttaggt
     2581 ggttactatg cctggattac ttgggatggg aaaaaactag accccgaact agcagaaaaa
     2641 gtagtagata tgggtcttgg aattgcacca agtctacttt ttgggcaaga tataaatgga
     2701 atcagaatca attacagtag gctggatgaa aaccaaatac cgttgtttta ttcaaagatg
     2761 aaaattctag aaaagtggtt aacagaataa tttcacaaag taaatagatt aaagaatata
     2821 acagcttgga tttaaatttc aagctgtttc ttttttttcg gaaaaagaat acgatataaa
     2881 gaaactaaga aaaaaccatt atttgtacgt ccgttcacga tgtgtaaata aaataaatgt
     2941 ttgcaacaaa actgtataac accaacgttt ttaaatagaa acagatggtt gtaagtttga
     3001 ttagaaaata gaaaaaatgc agatgaaata taaaacgctt tccttattgt gattttattc
     3061 actaatgctg ttaatatgcg gattggcata atttcctttc atcgtgaaaa tttgtgcttt
     3121 tagcttgtat ttctcataaa tccatgataa tttattaatg aagacggtta aatagtgata
     3181 cagttttatt cactgtttca atcgcagaat gaagagattt tcttatagat gagagaggaa
     3241 tgtgtgacaa atggcaaaca aaaaagcacc gatcgacttc caagcactgc tagatgaagt
     3301 caatgcagat ttcccagtat atcaagcact tgatcaagat ggaaacgtag ttaacaaaga
     3361 tttagtacct gatttatccg atgatgaact cgttgaatta atgactcata tggtatggtc
     3421 tcgtgtatta gaccaacgtt caacagcgct gaaccgccaa ggacgcctag gcttttttgc
     3481 accaactgct ggacaagagg caagtcagct ggctagtgct tacgcatttg ataaagaaga
     3541 tgttcttctt ccaggttatc gggatgtacc tcaactagta aaacatggtt tacctttatc
     3601 tcaagctttt ttatggtctc gaggacatgc tgcaggtaat tcttatccgg aagaattgaa
     3661 agctctacca ccacaaatta tcatcggtgc acaatatgta caagcagccg gtgttgcatt
     3721 aggtttgaag aaacgaaaca agaaaaatgt cgtcttcact tatactggtg atggcggttc
     3781 atcacaaggt gatttttacg aagcaatcaa tttcgctggc gcttaccatg caaatgccgt
     3841 attttatatc caaaacaacg ggtatgcgat ctctacacct cgtgaaaaac aaactgcagc
     3901 aaaaacactt gcacaaaaag ctgtcgcagc tgggattcct ggtattcaag tcgatggaat
     3961 ggatccgctt gcagtatata ctgtatctaa aatggcacgg gaatgggcga tcagtggaaa
     4021 tggaccagtc ctaatcgaaa cactgactta tcgttatgga ccacatacac tatctggtga
     4081 cgatccaacc cgctaccgta caaaagacac agatgacgat tggcagaaaa aagatccatt
     4141 gatccgcttc cgtaaatatt tgacggaaaa aggactttgg tctgaagaaa aagaagaaca
     4201 agtcatcgaa gcaactaaag aagaaatcaa agcagctatt gctgaagccg acaaagtacc
     4261 aaaacaaaag gtttcagatt tcttgaaaaa catgttcgaa gtatctccac aatcaatcaa
     4321 agaacaaatt gcaatttatg aagcgaagga gtcgaaataa tcatggcaca aaaaacaatg
     4381 atccaagcaa tcacagatgc cttagcactt gaactagaaa atgacgagaa tgtcgttgtt
     4441 tttggtgaag atgttgggaa aaacggagga gttttccggg caactgaagg attgcaggaa
     4501 aagttcggag aagaccgagt attcgatact cctttagctg aatctggaat cgcaggattg
     4561 agtttcggtc ttgcgttaga aggatttcgt ccagttcctg aaatccaatt ctttggtttt
     4621 attttcgaag caatggacga agtagtagct caaatggctc gtacaagata tcgaatgagc
     4681 ggcactagaa atctacccat cacaattcgt tcgccatttg gtggcggtgt tcatacacca
     4741 gaattgcatt cggataattt agaaggtttg atcgctcagt ctcctggtat tcgagtagtg
     4801 attccttcca acccttatga tgcaaaagga ctattgattt cttctattag aagtaacgat
     4861 ccggttgttt tcctagaaca catgaaacta tatcgctcat tccgagaaga agtgccggat
     4921 gaagcttacg aagttccttt ggataaagca gcagttactc gtgagggaac agatgtatct
     4981 attatcacat acggagcaat ggtgcgtgaa gcaatcaaag cagcagacaa tcttgcaaaa
     5041 gaaaatatat ctgttgaaat cattgatctt cgtacagttg cgccattaga tgtagaaacg
     5101 atcattcaat ccgtggaaaa aactggtcgc gtagttgttg tccaagaagc acaacgacaa
     5161 gcgggcgttg ccgcgcaagt agtttctgag atctcagaac gcgctatcct ttcattagaa
     5221 gccccaatcg gacgagtttc tgcacctgat acagtcttcc catttggaca agcagaaaat
     5281 gtctggttgc caaatgcaaa agatatcgaa gacaaagtaa aagaaatcgc ggaattttaa
     5341 gaaaagaagg gacgaaacaa agtcatggct tatcaattta aattgccgga tatcggtgaa
     5401 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
     5461 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
     5521 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
     5581 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
     5641 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
     5701 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
     5761 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
     5821 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
     5881 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
     5941 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
     6001 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
     6061 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
     6121 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     6181 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     6241 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     6301 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     6361 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     6421 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     6481 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     6541 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     6601 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     6661 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     6721 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     6781 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     6841 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     6901 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     6961 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     7021 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     7081 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     7141 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     7201 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     7261 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     7321 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     7381 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     7441 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     7501 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     7561 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     7621 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     7681 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     7741 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     7801 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     7861 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     7921 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     7981 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     8041 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     8101 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
     8161 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
     8221 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
     8281 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
     8341 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
     8401 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
     8461 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
     8521 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
     8581 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
     8641 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
     8701 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
     8761 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
     8821 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
     8881 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
     8941 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
     9001 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
     9061 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
     9121 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
     9181 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
     9241 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
     9301 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
     9361 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
     9421 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
     9481 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
     9541 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
     9601 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
     9661 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
     9721 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
     9781 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
     9841 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
     9901 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
     9961 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
    10021 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
    10081 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
    10141 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
    10201 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
    10261 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
    10321 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
    10381 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
    10441 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
    10501 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
    10561 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
    10621 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
    10681 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
    10741 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
    10801 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
    10861 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
    10921 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
    10981 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
    11041 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
    11101 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
    11161 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
    11221 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
    11281 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
    11341 tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac
    11401 gccgaaagac gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa
    11461 taagataatg tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg
    11521 gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag
    11581 tttagacctg gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga
    11641 atcatgccta ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat
    11701 gggcttactg tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg
    11761 aatcaattag gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct
    11821 agacgcttcc aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt
    11881 tttatcatgc cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt
    11941 gacgaggcac attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg
    12001 gcgaaccagc tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg
    12061 actgtgcaag tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt
    12121 cagactggtt ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa
    12181 caatatctaa tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca
    12241 acgagaaaag aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt
    12301 agatatcatg gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac
    12361 gatcgcttgc agctgattgt agcgacaaat gcatttggta tgggtataaa taaaagcaat
    12421 gtccgttttg ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct
    12481 ggtcgggcgg gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat
    12541 atccaagtcc aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa
    12601 gaatatgaaa aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa
    12661 tacatcctaa actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta
    12721 gatgacagag aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa
    12781 cgtatgggtg agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa
    12841 caaaagctgc aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa
    12901 tcgcaaaaag aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc
    12961 atcaatggtg agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga
    13021 caagaatctg tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc
    13081 gatacactat ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt
    13141 cccccgtatg tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca
    13201 agcagattgg agatgcttca gataaaagga gttggccaaa gcaagcttga taaatatggc
    13261 gaagcctttt tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa
    13321 cgaatacgta aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa
    13381 aatcaaatat gttataatta aggaattaga atgggaggta agcacagatg gattctttgt
    13441 cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc
    13501 gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata
    13561 cacagatgta cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa
    13621 agaacgaggg ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg
    13681 aggtttacca agaaatttat gataaaaaag aagttggcaa ggaggaatag acttgcctaa
    13741 gaaagtgaaa aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg
    13801 tgtattggga ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca
    13861 aaatccgtcc gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat
    13921 cgcactactt tataaaatga aaacagatgt tttgaaaaac cagcgtttga tcatggcagc
    13981 catcgcagtt ttgacgattt tgctgctgat tgttgtgttt tttggtaagg agatcaatgg
    14041 ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat
    14101 catttctatc tggtacctgt ctctgacttt atctaaaaga caaaacagtg tacaaaaaga
    14161 ttttcttggg actgtgaaac gtccacttgc aatggttatc ggacttactg cattagtcgc
    14221 tattcttccc gattttggta atgcggcagt tatctttttg attattttgg ttctgctttt
    14281 agccagcgga gtcaattatg tgtatacttt gattgtagga gtaggaggct tttgtctaag
    14341 tacttttacg atctggctga tcaatatcac aaacggaaag atcttacccg gacgtttaca
    14401 gtatatctat aatcgatttg ctatttacca aaatcctttc tcagacgaat taaataaagg
    14461 gcatcaacta gtcaacggct actatgccat gttcaacgga ggactatttg gcaggggatt
    14521 aggtaacagt atccagaaaa aaggattttt acaagaagca caaaccgatt ttatttatgc
    14581 aatcgtagtg gaagaactgg gagtgattat ggggattttg atattagcgt tgctattctt
    14641 tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca attcgctttt
    14701 gtgcattggg atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac
    14761 tggcgttatt cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt
    14821 gctgatgctt tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa
    14881 gagcttaggt ttataataaa aaaagatgta ggagtggttc gatgaaaaaa gttttagttg
    14941 ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa
    15001 ctgttggaat ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag
    15061 catatttagt aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca
    15121 tttctatcgc gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg
    15181 aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac
    15241 tgcatcattt agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg
    15301 ggattgcttc gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt
    15361 ttgcgaaaca atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg
    15421 gcatgcgtgt agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg
    15481 aagcaaaagc agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta
    15541 agcatatcga agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa
    15601 gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta
    15661 tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg
    15721 ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg
    15781 aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata
    15841 tcgtaacaac gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc
    15901 ctcagcaaga aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag
    15961 acccgttgaa taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg
    16021 gttttggcgt acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt
    16081 ttgattcatt gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga
    16141 aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta
    16201 tgctcaatgt catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg
    16261 atagtacagc aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga
    16321 aatatatcgg tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat
    16381 tttatgaaga accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta
    16441 agaatgtatt agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa
    16501 atttgttgtt gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg
    16561 ttcggacaaa ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct
    16621 tttcaagtga gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag
    16681 atccttggca acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc
    16741 ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat
    16801 ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact
    16861 ggactccaca aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg
    16921 cagctatttg ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt
    16981 attataaaga catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag
    17041 atatggctgg gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa
    17101 cgacagatct gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt
    17161 attctgctgc ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg
    17221 ggaatactag tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc
    17281 tgccagaaat cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca
    17341 tgtattacaa accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg
    17401 aaatgccagg aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc
    17461 atcgctggga tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg
    17521 ttaaagtaac tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg
    17581 atcttacaga agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg
    17641 tgactttctt ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa
    17701 agattattct taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag
    17761 tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag
    17821 acgaagtatt aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata
    17881 atcaatttgg cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg
    17941 gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa
    18001 tcggagaacc cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac
    18061 gtgaaatcac tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg
    18121 aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag
    18181 tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa
    18241 tggaaacggc catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag
    18301 gagacactat ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag
    18361 gtggggaaac atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc
    18421 attctacatg gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca
    18481 acaaatatca aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc
    18541 tacttatatc ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg
    18601 attaactagc ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac
    18661 atttgaagcg aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc
    18721 tgctgatatc gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac
    18781 tatctatact aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga
    18841 agaggatatg aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt
    18901 ttttgatcaa acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt
    18961 gaaactattg ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc
    19021 ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat
    19081 gcttagaaga gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag
    19141 tcagctgcta ctacatgatt ttttgacaag gcctgaggaa ataatgtcag aggaacgtat
    19201 ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa
    19261 tgatgaattg gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat
    19321 tcatccggcg attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca
    19381 tgaccgtttc atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat
    19441 gttagtctta ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag
    19501 agctgtttca actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa
    19561 cgatggcttc ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt
    19621 tgaaagaagc gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt
    19681 atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata
    19741 tggatgagca tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc
    19801 aaattgtcca agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc
    19861 cgttttttga aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat
    19921 agtgaatttg aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa
    19981 ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc
    20041 gtgctttact taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat
    20101 tttgttcgaa acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa
    20161 ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa
    20221 atccgattag ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac
    20281 ttggataacg atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt
    20341 accatttcta aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt
    20401 aaaaagttta gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt
    20461 caatatgatt ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg
    20521 tggtttagct atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa
    20581 ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt
    20641 taccacaata aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca
    20701 attcgactac ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca
    20761 taaaatggaa gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa
    20821 aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg
    20881 cataacggcc gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca
    20941 ggaagttttg atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta
    21001 ttcgatgagg tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca
    21061 gaagaaagaa tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg
    21121 catcaagaaa atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc
    21181 agaggcatac gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat
    21241 catctatatg atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg
    21301 agttccagta ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg
    21361 ccagtgatca acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt
    21421 gaaaacagta ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt
    21481 gccgtattat gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa
    21541 tgataaggaa gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc
    21601 tacaggtttc ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga
    21661 agaattgact ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga
    21721 ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat
    21781 ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct
    21841 ttcagttgga gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt
    21901 tatggattat gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa
    21961 tggtgaagaa aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg
    22021 aataggtatc acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa
    22081 ttctggctct atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca
    22141 agtcactggt aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag
    22201 tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc
    22261 tggcgtagag attttctttg caccagatga tacgatttca gacgatgtta agaaagaata
    22321 tcctgaaata aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc
    22381 aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa
    22441 atgaaactaa ataattaaag aggggttgtg acacgagttc tgtcacaacc cctctttttc
    22501 gtcaaaaatc ttcacgataa aaataaaaac aaaagaaaaa attgacgtaa ttagatgttg
    22561 aagggacgat tttgccagag ctcactgttt cttcaatttt aaatgaaagg agtaatgaat
    22621 gatttttact ggtcattctt tgaaaaaaga acaatgatag aaaaaatcag caagaaaata
    22681 ctagcttcac ccaaattgat catttggatg tctatgatag ttcttatctt ttgtatcgca
    22741 agtggttttt tattatcaaa tttgctattt tatagagaaa cagaagagac tgctgcaaaa
    22801 gaaatacttt cttcgtttag ttccagtgga gaagatccat cagctgaagg aaacgatgta
    22861 gaagaggatg ctcgtccaaa ggtcatgtac accgatatca aaggaagtgt gaaggagcct
    22921 ggtatctatt ccttttcttc agaagaacgg gtctatgatg tactaaagcg tgcaggtgga
    22981 ttattagaag aagcggatag cgatcgaatc aacttttctg cgaagataga agatcagcaa
    23041 gtcctatata tccctgctgt aggtgaagag ctgccagaac atcttgatca gttggcatcc
    23101 tcggaaggaa aacagcctac agcagattcc gagcctccca aaataaatat caatacagcc
    23161 actccatcag aacttcagca gattcctggg atcggatcag tcaaagcgca agaaatcatt
    23221 cgatttagag aagaaaacgg atccttccag aaagtggaag acctgcagga gatttctggt
    23281 atcggtgaaa agacagttga aaaactaaaa aacttcgtta caattaaata gaatgattca
    23341 aaatcgactg gcacgagcaa taaatggacg ctaatggata ctaagaataa tccaattcta
    23401 agagcttggc aggacgagga ggtagtattt ttggatagga aatagcttga ttgttcgcaa
    23461 agaaacatta aaaaaaccaa tgctgaatgt ctatctccaa aataaaatat ctgggattca
    23521 tattatgaat acggctgtca gcggcaataa cagtcaagct ctacgagaac gctttgccaa
    23581 agatgtactt agctatacag cagataaagt atttattctc attgggacga atgatctagc
    23641 agagcataaa caactttcta aagaaacgta ccaaaaaatc tgcagtggat gatagttcag
    23701 ctgaaaaaag actatagttc tgatcatgct ttctttatca cgccaccagc agtcgatgag
    23761 acaaaacaaa aatatcggaa taacaagttg atcaaagaat atatctctgt tctcacagaa
    23821 gtagcaaaac gccatgattg tgaagtatcg atttttatac ggaatttctt gaacaaatgg
    23881 attcgaagaa tttttataat gggctaatgg atgacggact tcatttcggc agtgaagggt
    23941 aggatctttt gtcagacagg atgattcgtt atatttaaag aatattttgt ttaagcaatg
    24001 agtacttttt aatcaacttg aaaaaacgga ggaatataaa acatgttaga aaaatcttca
    24061 aaagcatgcg atacgaataa agaaaaagtt actgataagt cacataatga acgcattcct
    24121 tgggaccagt actttatggc tcaggcagta ctgttatctt tacgtagtac gtgtacacgt
    24181 ttagaagtag gagcgacact cgttcgagaa aaaaggatca ttgctggcgg ctataatgga
    24241 gcagtcagtg gagatgtcca ctgcatagat gaagggtgtt acgtcgtgga tggacactgc
    24301 ttgcgaacga tccatgcaga gatgaatgca ctcctacagt gtgcaaaact tggtatcccg
    24361 acggaaggtt cagaaatcta tgtcacacat tttccatgtt tggcctgtac aaaggctttg
    24421 ttgcaagctg gtgtgaaagg gatcaattat ctccatgact atcgaaatga tccttatgcc
    24481 caagcgttga tcgaacaatt aggtgtatct gtacaccaag ttgctttaga ctcacagcat
    24541 ttttcaaaac ttcaaaatga attaacagga aaacatgtct agcgttttag aaaggcatgt
    24601 tcgaaatcgg ttgatttttc cggcaattac gtcgatgatt atctgtttgt tgtattacac
    24661 tgacaattgg ggttatcgtt tgcttcttgc cggaattttt ttatggtttt cgctaaaaga
    24721 aggacgtttt atcgcgtact tgtgtatttt gtgtagcata acggtatcca tttcttatag
    24781 gctacaagag tattcatttt tagaggaaaa aacaacatct gcgcaaatta ctgtatttac
    24841 agatacgatt cgtgcaaatg gtaatttttt gacaatggaa ggaaggttat cagagaataa
    24901 acaaaagctc cagctttctt atcaagtaaa cacagaagca gaattgaaaa catggcttaa
    24961 cttccgagga aagggaatag aattgactgc tgaaggcgag ttcatctctc cagcaaaaaa
    25021 gcggaatctt tataattttg accaagaagc ttacttcagg aaccatagaa tttctggacg
    25081 gttccaaatt caaaagattg aacagatcac catgcaaaat cactggcgaa actggcttca
    25141 gagaaaaaga ggatctttta tctcctttgt ccaatctgaa tttccgcaaa aaacaagtgt
    25201 atatattaat tctttattat ggggctataa agatgcagga ttcagagaac cagaagaaat
    25261 ttttagggag agtggtttgc tgcatctgtt cagtctatcg ggtctacaca tccaatttta
    25321 tttagggtgg ctgtattatt tatttcgacg tatcggatgg ccactacacg tcagtattat
    25381 tccgttgagt ctactaacta tctgttatgt ttctattgca ggaagttcta tttctgtttt
    25441 gcgtgctagt ttgctgtttt tattgcgatt gctagtcaaa ttgctgaata agcattattc
    25501 caccatggac ttatttgcag ttgtcttttg gcttctttta ctatttgatc ctcgatttct
    25561 ttttcatgcg ggtggacagc ttagtttttt catgagtttt ttgtttatcc tgatttcttt
    25621 tgatggagat tggttgaaaa aaacggcctc tcacgtcctg tttactatga tcacgatgcc
    25681 gttgattgta tggcattttt atgagtggcc tattttaggt agtcttttta cattactgat
    25741 tgcaccggta ttcaagtttc tttttcttcc tggtgtgtta tttctcacac tcttcaatcg
    25801 ctggctgccg gaaactttac ttttgctgat agaagaggga ttaatcttat ttgagcgaat
    25861 aatcgatttt tcttcagggt cgtctctcat tataggacgt ttgccccttg tgctatctgg
    25921 gatgtatatt ttcggattac tgatttgtat ggataggttg aaagaacaac ctgtgaaaca
    25981 cttgttttat gttggtcttt tttcttttat ttttttgact ttgccatttt tacgcttttc
    26041 ttcaacgatc gcttttatcg atgtcggtca aggagacagt atctttttgc aaagtccttt
    26101 tcgaaaagaa acgattttga tcgatactgg aggtcagtta cactttgaaa gagaaaagtg
    26161 ggcgcaaggt atgatacgtc ctcctgctga aagaaatatc gttccatatc taaaaggtca
    26221 ggggataagt gtaatcgata aactcatctt gacacacgac gatacggatc atgtgggaga
    26281 attacccacg ttatctcagc attttactat caaaaaaatt tatatcggtt ggggcgctgg
    26341 acgaaatgag ccattacaaa ggcatttatc tgaagctcaa aaaaatggta ctgagatcat
    26401 tgaaatcaaa catggggaca ggatcagcgg ttattatgat ctttatgtat tgactccttt
    26461 tgaaaaggga gagggaagaa atgaagattc gattggtcta tggatggaat ataacaatag
    26521 gcgcttttta tttttaggag atttgaatca agcaatggaa aaacaattgc ttttgattta
    26581 tcctaatctg aaagccgatg tagtcaaatt aggtcatcac ggtagccgta cgtcaagtaa
    26641 cacagatttt ctttcccata ttgaagcaaa acatgggatt atatcctgcg gcgtaaataa
    26701 tcgttatggt cacccgcaca gcgaagtatt ggagacacta gaagaaaatc agataaaaac
    26761 atggcgaacg gatcaacaag gaatgatcac ctatcgctgg catccagttt ttcatcctca
    26821 tgggctagtg gaaacaatga tagactagta attctcatct gctcatggta ggataatttt
    26881 tgaaagtggt gagtaatttg agtatacaag cagagttaca aaaaatacgt aaagaaaaac
    26941 ttgcatcttg ttacttgatt ttaggaacag aaaagtttct ccaagatcaa gttcggacag
    27001 aaattgtcaa aaaaatccaa ttagagggcg aagatgactt aaatttttta agttttgaca
    27061 tggaaaatgc aagtttagat gaggtagtag ctgaagcaga gacgttgccg tttttcggag
    27121 atcaacggtt agtattcgta gagaatcctt attttttaac aggtgaaaaa gtaaacaatg
    27181 gaatcgaaca aaatactgat ttattgacgg cttatttgaa agaaccatta gaatcgactg
    27241 tattagtatt ctttgcgcca tatgaaaagt tggatgaacg gaaaaaagta acgaaacaat
    27301 tgaaaaaaac agcaattatc atcaatgtcc aacagttgaa tgaaaaagaa gtacgtcaat
    27361 atctgatgaa taccttagag aattcctcaa tcaaaatgga acgaagtgca attgatttat
    27421 tcttgaggtt gaccgactta gatttatcca aaatgatgag agagttacag aaattggtcc
    27481 tttatggaca aaatcaacag caaatcacgg taagagaagt agaagaatta gttcctaaaa
    27541 cgttagaaca taatattttt gacatgaccc aatatatttt aaaagggaag acggaacaag
    27601 ctttgcgttt atacgaagat ttagttatgc aaggggaaga aacaatcaaa attaatgcga
    27661 ttttgctttc tcaattacgt ttatttttac agacaaaatt tcttgtaaag attggttatc
    27721 agcaggcaaa tattgctgaa acattgaaaa tccatccata tcgtgtaaaa cttgccgtgc
    27781 aggaagtaaa aaaattcgat gaacatttgc tggtacagtt gtttgatcaa ttagttgaga
    27841 tggattatca aatcaaaacg ggacaaatcg aaaaagaatt aagttttcaa ctattcgttt
    27901 tgcgcacagg gcagttgtta agaaatcgat aagaagaaag acgagggcaa agcatgcggg
    27961 aaggtcgttt gtttttgtcc tttttttgac aaataaaaaa atcggccgaa gccgattctt
    28021 ttatttagcg atttttttgc ttaaacgaga tttgtcgcgg ttagctttgt ttttgtggat
    28081 tagacctttt gattcagcca tatcaatagc tttaacagct tctttatata acgcatctac
    28141 gttgtcagca cctgaagcta cagcatcttc aaattttttg attgcagtac gcatagcgct
    28201 tgtttgagat gaatttttca cattagcgtt ttcgcttgta cgaacacgtt tgattgcaga
    28261 ttcaatgttt ggcattagtt tcacctccga taacttaagt tcttacgcgt gttaccgcat
    28321 aattcaacat tcattattat acctaaaggc agcgtgcttt gcaatatgaa catgacaagt
    28381 ttttttcctt aacagtcttt tattaggatt ttttttcagt aaaaacggat agtacaagct
    28441 gaaaaaaatc aaaaagacgc tattgtggga ggcaatagcg tcaaaccaaa ctagattttt
    28501 atttatctat ttaaacaaag tttgaatggg aatgtttggt actaagttat tatataacaa
    28561 agagaaccgt ttgcaaagtt ttttttgaaa agggatacga ttccctgttg ttctttgttt
    28621 ttctattaag aaactgcagt tattttataa ccagtatcct ttgatcagta aaggctattc
    28681 tggtgtaccg attattttaa tgtattcatc gatatttttt atcaacattt tttttatgac
    28741 caactggttc cgacggttcc atttgccact tacttccagc acatatttat ttaggggcaa
    28801 acgtaagata tattttgcca aatagcgatc tttaagcagg ccattgatca cacggtcctc
    28861 aagctgcaag ttgatcccag tcagcatcat tggctgttct tgtaaaacgt acgtatcaat
    28921 taaaagacct tcaaaagttt ccacaagcgt cttcctttct tttcaaagta acctgaaaac
    28981 aggcactaat tcttccattt tgatattcgt tgaagtggat gcgatcattc tttcttttgt
    29041 ccactgtaat ctagtataac aaaaaagagc agaagagcca aaaagaaaaa agagcatcga
    29101 attctatagg aagtctcatt ggcacggcct aaattattac aaaacattga ttaaaaaaag
    29161 cccctgcatc gcaggagcat cccctatatc gttttaatag gtcaatcaaa aagcagtatc
    29221 aaacacattt tgtatcaaat caggaaataa tagataagct aagcccagaa tcaaaagtac
    29281 cgctagaaga aaccagccga ttgttcctat gaaatacttt aatttttcga acattgtatc
    29341 acctcgttgt cagtatatca cggatcagta aattgggggt gttaagaatc tcctaaaata
    29401 gacaaaattt gttctatttt cctaaagcaa atacttgtaa gatacagtaa gtgataccag
    29461 aagcaatgac cggtccggcg gcgattcctc ttaagaagac aacacctaaa atcgtgccga
    29521 aaacaagtgc aacagtgatt tgtggatcag aagcaatcaa tcctacaccc ttggaagata
    29581 agacagccac tagcccaccg caaaaaacag ctacccagcc aataggggat ttaaaggctt
    29641 ctaatagatc acgtagaccg atttctcctg ttgcgatagg gattagaata gccgctgtga
    29701 tgatcgtcac cccaatattg attcctttgc cttgaagcca agggaaaaag gaacgggagg
    29761 ctggtatgag cttcataatc aaaacaaaag ctgccgctat catcagactt tgatttttag
    29821 ctaaaattgc tacaagtaaa ataattccta aaaaaagcca actttccatg aaatatattc
    29881 ctcactttct tcgaaacaat cataacatgt ttttagaaag ttacaaaagt aggaattcat
    29941 ccttcaagac ctattcataa gctgtttttt tcataaaata aacaaaatca taaaaaaaca
    30001 ctttgcttat ttgacagaag acagccgcgc tttgtatagt ggacaagact tacatttttt
    30061 tgaagaatca gaaagatcag gtaaaggtct tagtaaacgc aagccattca gaataaccaa
    30121 tattgtgctt ccttcgtgtc ctacaactcc aagaggtaga tttaaaaatt gcaggaagtt
    30181 agaagcaatc aacaagagaa taacaagaat cgaaaaaata atattttgtt tgatgatccg
    30241 ccgcattttt aaagatagcc gatgactgta gacaaattta tttaagtcgc ttttcattaa
    30301 gacgatgtct gcgatatcca tagcggcatc tgtcccttgc cccatagcga cacctaaaga
    30361 agcggctgct aaagcaggcg catcattgac tccatcacca accatcgcat ttacttggta
    30421 aagttcattc tcttttttga ctagttgtgt tttttcttcc ggagtacagc ttgcatagta
    30481 atgttcaatc cctacttgtt cagcaatggc ctttgctgtg ccagaatggt cgccggttat
    30541 catacttgta tggatgtttt gggatttaaa gtaggaaatt acttgttggg tatttgcttt
    30601 tggaatatct agcagaccta aaacagcaat taactgctga tctttagaaa gataaatcac
    30661 ggttttgcct tgattttcca aacgttcgat catttccgca atctctttgc tgattatcat
    30721 cgtctcagga tcatatgcgt gcttgccaac tttccattgc gcacctaaat aggtagtttg
    30781 taatccaaaa ccagtaattt cttctattgg taagtattgg atttcttcag gtattgtgca
    30841 atcgaaatat tgcaaaatcg catgagcaag tggatgcgtc gttttttgtt ccatagcaac
    30901 aagaagttgt tgtgccaaat ccttttcatc aaagaaaaag ctgtcagtta caactggttt
    30961 tcctttagtg agcgttcctg ttttatcaaa ggcaatcgct tttaattcag aaagcttttc
    31021 taaatggata ccgcctttga ttaaaatccc attttttgca gaatttgaaa gagcagctag
    31081 agaagctggt gtagcagatg cgacaagcgc acaaggagaa gccacaacta ataataccat
    31141 tcctcgataa aagctttcat cccaactcca acctaggaaa aagtggggga gaaggatcat
    31201 tagaggaaca gcaagcaaaa tcaattttac ataattattt tcgattcgtt cgataaatga
    31261 agctgtttga gttggcgtat tctgcgcttc ttctactaat tggataatct tagcaaagac
    31321 agtttcggaa ctcgtttttg ttactcgaat cgtgatggga tttccttgat tcagcgttcc
    31381 tgcatacatc tcatcgccta aatatttttc tacaggcaca gattcaccgc ttaatgcaga
    31441 ctcatcgatc gttgcctgtg tgctttctaa aaagccatct atcggaacag ctgctccttt
    31501 agcgacaaat accaagttat ccacctttag ttgagaaaca tctacttctt gcatttgtcc
    31561 attttctttt aataattggg ctttttgtgg ttgaagattc atcaaagcag taatttcttt
    31621 ttgactttta ttagttgtgt attcctctaa tgcaccgctt aaacaaaaga tgaatgtcaa
    31681 cattgctcct tcaaagtaat taccgatcaa acaggcacca atagcagcta aagccatcaa
    31741 taaatccaca ttcagtgtcc gatcattcca caattctttc aagccttccg atgtttgttt
    31801 atatccaccg atgataatgg cactgatata aaaaagtggg gtgatggtaa gagagaatcg
    31861 gtcgaagata aaaccgaaga tcataaataa aaggcagccg attgttgctg caaaagcttt
    31921 tttctgatta gtcatcgtta ttagacctcc ttgtcaaaac aaggatagca taataa
//
LOCUS       DAJSMI010000013        30606 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJSMI010000013 DAJSMI010000000
VERSION     DAJSMI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.013"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            147..1016
                     /locus_tag="ON430_000517"
     CDS             147..1016
                     /locus_tag="ON430_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCR3218463.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ON430_000518"
     CDS             1225..2250
                     /locus_tag="ON430_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR3218464.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ON430_000519"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ON430_000519"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCR3218465.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="ON430_000520"
     CDS             3333..5036
                     /locus_tag="ON430_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3218466.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ON430_000521"
     CDS             5038..7071
                     /locus_tag="ON430_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3218467.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ON430_000522"
     CDS             7082..7522
                     /locus_tag="ON430_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3218468.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ON430_000523"
     CDS             complement(7722..8849)
                     /locus_tag="ON430_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218469.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ON430_000524"
     CDS             complement(8852..9529)
                     /locus_tag="ON430_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218470.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ON430_000525"
     CDS             9793..11559
                     /locus_tag="ON430_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR3218471.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ON430_000526"
     CDS             11574..12011
                     /locus_tag="ON430_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3218472.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ON430_000527"
     CDS             12024..13175
                     /locus_tag="ON430_000527"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR3218473.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="ON430_000528"
     CDS             complement(13237..13935)
                     /locus_tag="ON430_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCR3218474.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="ON430_000529"
     CDS             complement(13928..15280)
                     /locus_tag="ON430_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCR3218475.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="ON430_000530"
     CDS             complement(15503..16903)
                     /locus_tag="ON430_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCR3218476.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="ON430_000531"
     CDS             17064..17801
                     /locus_tag="ON430_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR3218477.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="ON430_000532"
     CDS             17926..18405
                     /locus_tag="ON430_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCR3218478.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="ON430_000533"
     CDS             18402..19169
                     /locus_tag="ON430_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218479.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="ON430_000534"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="ON430_000534"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3218480.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="ON430_000535"
     CDS             complement(20072..20674)
                     /locus_tag="ON430_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3218481.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21249
                     /locus_tag="ON430_000536"
                     /pseudo
     CDS             20780..21249
                     /locus_tag="ON430_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21355..21939
                     /locus_tag="ON430_000537"
     CDS             21355..21939
                     /locus_tag="ON430_000537"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR3218482.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="ON430_000538"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="ON430_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR3218483.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="ON430_000539"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="ON430_000539"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR3218484.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="ON430_000540"
     CDS             23840..24019
                     /locus_tag="ON430_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218485.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="ON430_000541"
     CDS             24041..25066
                     /locus_tag="ON430_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR3218486.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25241..26485
                     /locus_tag="ON430_000542"
     CDS             25241..26485
                     /locus_tag="ON430_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR3218487.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26638..27585
                     /locus_tag="ON430_000543"
     CDS             26638..27585
                     /locus_tag="ON430_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3218488.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27719..28348
                     /gene="upp"
                     /locus_tag="ON430_000544"
     CDS             27719..28348
                     /gene="upp"
                     /locus_tag="ON430_000544"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR3218489.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28487..28729)
                     /locus_tag="ON430_000545"
     CDS             complement(28487..28729)
                     /locus_tag="ON430_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218490.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28850..28922
                     /locus_tag="ON430_000546"
     tRNA            28850..28922
                     /locus_tag="ON430_000546"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28883..28885,aa:Thr,seq:cgt)
     gene            complement(29041..29679)
                     /locus_tag="ON430_000547"
     CDS             complement(29041..29679)
                     /locus_tag="ON430_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3218491.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29802..30434)
                     /locus_tag="ON430_000548"
     CDS             complement(29802..30434)
                     /locus_tag="ON430_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR3218492.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT        10419 a         5380 c         6178 g         8629 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt
    25261 tgaccctgat ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga
    25321 gctgattgcc tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt
    25381 gacaaataaa tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt
    25441 cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc
    25501 gaacgtccag cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga
    25561 accaggggac acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc
    25621 accagtcaac ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac
    25681 agaagttatt gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat
    25741 cgtagcaggt gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc
    25801 agatgaagta ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc
    25861 aggacttcat cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac
    25921 attgcgtggc cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat
    25981 aaatagtgcg gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa
    26041 agcagtagca ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat
    26101 cgccaatgca aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc
    26161 aggggcgaca gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa
    26221 agaagcagag agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt
    26281 tgaaacacta agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac
    26341 tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca
    26401 agcaaaagat gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga
    26461 aaaatttccg ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa
    26521 tgttgggctt ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct
    26581 ctctagtata tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg
    26641 actgatcgta tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat
    26701 ccgcgtatct atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa
    26761 tcacctgtga acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg
    26821 ggaaaactca atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt
    26881 ttttatattc atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc
    26941 agagaacttg cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca
    27001 gaagcaaaat atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa
    27061 ttagccaagt caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt
    27121 ggtaacatgg ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc
    27181 aagcaattgc tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa
    27241 ttcggagaaa actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg
    27301 acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa
    27361 ctaaatgatc ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa
    27421 ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc
    27481 caaggaatca tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt
    27541 gcagcaatgg cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc
    27601 aggaaaactt cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa
    27661 tcacttgatt ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat
    27721 gggcaaattt caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga
    27781 caaaaactgc ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc
    27841 atatgaagta tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga
    27901 aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg
    27961 tattggaatg gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg
    28021 tctttatcga gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga
    28081 cattgatgca cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat
    28141 catggcaatc gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt
    28201 agcagcacct gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac
    28261 agcttctcta gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc
    28321 gggtgaccgt ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt
    28381 gtcacatcct tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc
    28441 gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta
    28501 gttctttgta gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg
    28561 cagtttcatc aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt
    28621 gcgacttgcc gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt
    28681 ccgatggtac gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct
    28741 ttcacatcaa ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc
    28801 ggactcttta caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc
    28861 tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg
    28921 cattaaaaga gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt
    28981 gtttaaagga aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat
    29041 ttaggagaat aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca
    29101 acttgtaatg atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc
    29161 ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta
    29221 atggataaat ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact
    29281 agaaaaaata gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa
    29341 aataataaat aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt
    29401 caaaatttgt tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata
    29461 tttggctata tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt
    29521 accaaaaaca agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat
    29581 ggatggcgca aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc
    29641 ttttttaagt tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca
    29701 gtttttgaag atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga
    29761 tatttgtatt gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt
    29821 aaaaattttc tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt
    29881 tccgagcgta cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca
    29941 gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa
    30001 cagtctacaa aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt
    30061 gaaccagata aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa
    30121 ctttccatca atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg
    30181 ctttttgtca atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc
    30241 attctttgaa aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt
    30301 ggatggcaga ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca
    30361 aaatcttcgt tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact
    30421 ttaggaaaaa tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa
    30481 ccaacttaca ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg
    30541 ctatctcttg aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat
    30601 aaaaat
//
LOCUS       DAJSMI010000014        28876 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJSMI010000014 DAJSMI010000000
VERSION     DAJSMI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.014"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..198)
                     /locus_tag="ON430_000549"
     CDS             complement(<1..198)
                     /locus_tag="ON430_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCR3218493.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWE"
     gene            complement(195..713)
                     /gene="msrA"
                     /locus_tag="ON430_000550"
     CDS             complement(195..713)
                     /gene="msrA"
                     /locus_tag="ON430_000550"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3218494.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(733..1446)
                     /locus_tag="ON430_000551"
     CDS             complement(733..1446)
                     /locus_tag="ON430_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCR3218495.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1449..2345)
                     /locus_tag="ON430_000552"
     CDS             complement(1449..2345)
                     /locus_tag="ON430_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3218496.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2503..3345)
                     /locus_tag="ON430_000553"
     CDS             complement(2503..3345)
                     /locus_tag="ON430_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3218497.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3477..4130
                     /locus_tag="ON430_000554"
     CDS             3477..4130
                     /locus_tag="ON430_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR3218498.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4193..4720)
                     /locus_tag="ON430_000555"
     CDS             complement(4193..4720)
                     /locus_tag="ON430_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3218499.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4740..5687)
                     /locus_tag="ON430_000556"
     CDS             complement(4740..5687)
                     /locus_tag="ON430_000556"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR3218500.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5699..7585)
                     /locus_tag="ON430_000557"
     CDS             complement(5699..7585)
                     /locus_tag="ON430_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3218501.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(7745..9823)
                     /locus_tag="ON430_000558"
     CDS             complement(7745..9823)
                     /locus_tag="ON430_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCR3218502.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(9836..10111)
                     /locus_tag="ON430_000559"
     CDS             complement(9836..10111)
                     /locus_tag="ON430_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218503.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(10473..10928)
                     /locus_tag="ON430_000560"
     CDS             complement(10473..10928)
                     /locus_tag="ON430_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218504.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(11082..12290)
                     /locus_tag="ON430_000561"
     CDS             complement(11082..12290)
                     /locus_tag="ON430_000561"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCR3218505.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(12402..12734)
                     /locus_tag="ON430_000562"
     CDS             complement(12402..12734)
                     /locus_tag="ON430_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR3218506.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            12882..13757
                     /locus_tag="ON430_000563"
     CDS             12882..13757
                     /locus_tag="ON430_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3218507.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(13839..14420)
                     /locus_tag="ON430_000564"
     CDS             complement(13839..14420)
                     /locus_tag="ON430_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCR3218508.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(14426..15685)
                     /locus_tag="ON430_000565"
     CDS             complement(14426..15685)
                     /locus_tag="ON430_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR3218509.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(15687..16733)
                     /locus_tag="ON430_000566"
     CDS             complement(15687..16733)
                     /locus_tag="ON430_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218510.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(16968..17243)
                     /locus_tag="ON430_000567"
     CDS             complement(16968..17243)
                     /locus_tag="ON430_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCR3218511.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(17486..18796)
                     /gene="der"
                     /locus_tag="ON430_000568"
     CDS             complement(17486..18796)
                     /gene="der"
                     /locus_tag="ON430_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR3218512.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(18978..20207)
                     /gene="rpsA"
                     /locus_tag="ON430_000569"
     CDS             complement(18978..20207)
                     /gene="rpsA"
                     /locus_tag="ON430_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR3218513.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(20342..21022)
                     /locus_tag="ON430_000570"
     CDS             complement(20342..21022)
                     /locus_tag="ON430_000570"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR3218514.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(21071..21706)
                     /locus_tag="ON430_000571"
     CDS             complement(21071..21706)
                     /locus_tag="ON430_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3218515.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(21772..23175)
                     /locus_tag="ON430_000572"
     CDS             complement(21772..23175)
                     /locus_tag="ON430_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR3218516.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(23162..24193)
                     /locus_tag="ON430_000573"
     CDS             complement(23162..24193)
                     /locus_tag="ON430_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3218517.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            24224..24445
                     /locus_tag="ON430_000574"
     CDS             24224..24445
                     /locus_tag="ON430_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCR3218518.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(24610..25347)
                     /locus_tag="ON430_000575"
     CDS             complement(24610..25347)
                     /locus_tag="ON430_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCR3218519.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(25500..26840)
                     /locus_tag="ON430_000576"
     CDS             complement(25500..26840)
                     /locus_tag="ON430_000576"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCR3218520.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(27077..28600)
                     /locus_tag="ON430_000577"
     CDS             complement(27077..28600)
                     /locus_tag="ON430_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCR3218521.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         8073 a         6087 c         4892 g         9824 t
ORIGIN      
        1 ttcccatgct tcgtcaaaga tatccatgga cggcaaatag tcgacattca gttccagata
       61 attagaaatt tcttcgtagc tagagctgtg cttaggaaat tggatgtctt taccggcttc
      121 tgtagcgaaa acagataaag catcatgtgt gccaccgcct cgcagtgtca ttaaataatg
      181 aaaaaagctt cgattcaccc ttcgttcctc caattttctt cgataaaggc agcccggcgt
      241 gcatggtttc ttttaaaatt ttcaggatgc tttttataat actcttgatg gtcttcttct
      301 gctagataaa atggttgggc tggttcgatt tttgtaacga ttggctgttc gaaacgtcca
      361 ctttcagcta actgtttctt acttttttca gcaatgatcc gctgttcttc tgtcaaataa
      421 aagatcaccg ggcgataatt gtcaccgcga tcctcgaatt gtccgaaagc gtcagttgga
      481 tcagtttgct gccagtaaag ttctactaat tgttcatatg aaatcaatgt tggatcatag
      541 ataatctcca ctgcttctgt atgtccagtc gtttggcttt ttacttgttc ataagtcgga
      601 tctggtacat gccctccagt atatcctgac atcactgtat ggataccagg aagcgtgtcg
      661 aaaggctgga tcatacacca gaagcagcct cctgcaaata ttgctcgttc cattatgatt
      721 ccctctctaa tactactcat ccgatgattt tttattttct tttgtcttgc tttcagtact
      781 gctggtgcta gttttgttgg tatttgagtt gtttgaagca tcagaggtat cttttggcaa
      841 atatagacta aaccgaatgt catcatttac taagtcgatt ttatctgctt taatatacat
      901 cccgttttgc attcggaatt gatccaacct aagcatgata gtcagatcat ctgtattgac
      961 ttcgatccaa tctggtaatt tatagtttct cttgatcata ttcatcacat ctttgattgg
     1021 caaactcaat gttccgatag aaagacttct agcttttagt tggacatttc cgttttccat
     1081 cacatacgga tcaaaatata aatagaaatc gataggaaaa cctagtactt cgaaagtacc
     1141 attgagtagt gcttcatttt ccaaataaaa tttatattca atatcactat ctttttgata
     1201 ctcactaagg aaaaagctga tgatttgatt gactttttct ttatttgaat tgatcgttaa
     1261 gacaggatct ccttgtcttt ccgtaagagc tggtatttct gggagttctg gttcccgatt
     1321 agcaaaaata cggctgccta aaaatacggt cgaaccaagt actaccccaa gaaggataag
     1381 gaaggcgaat ttccagtaat ttgttttctt tgttttgttt gaagatacgt cagatcttgt
     1441 tttcatgttt attgatcatc ctccttctct aaccattggt tttgtgtctc gatcagctga
     1501 tctctcactg catttgccat caattgataa cctaaattat ttggatggaa atgatcttct
     1561 tcatagagca cattattttt gatgccattt agattttctg tcgtgctagt tgtttgatct
     1621 gttccttctt ctgtgacacc tacttcctga ttcaatcctt gataaagcaa atcattgatt
     1681 gggataaagt agcagttcgg catttttttt gtcaccgttt ctgttccttt gttccaattg
     1741 ttaacaattg tctgcatgtc agtgatctca ggaaagttca aataaaatgg attgtagatg
     1801 cctaaaatgt aaataggtgc ggttttattg agggatcgaa attcttccaa tagttctgtg
     1861 acattttcct gatagttttt cagtggcttt ttaaaggaat tgaccgttaa cccgaataaa
     1921 ttattttgga taactttcat caaatcatta ccgccaacag tcagtgtgat aaaatcagct
     1981 gttttgatat tgtttcgaat ttcttctttt gtttttactc gctttaagat ctggtcactt
     2041 cgttcaccat ttacaccata gttttctgtt tcaaccgcgg tcaaattata acggtctttt
     2101 agatcatttg cgacaagggg aacgaatcct ccttgcttgg tttgatctcc tactccttca
     2161 gtcagcgaat ctccgatagc tatgtagtga agtttttctt ttttatttgg atcttctctc
     2221 acagtagtta ctggtttcat tagtgggcca gctttcggta tagaagcagc taaacctgca
     2281 tagatgatga tcgcaattaa taatgggaac aaaacgagga tcaaatgtcg tttcgttaat
     2341 ttcatttttt cacctcttgt attcgattgg ttctaatgtt atcaaaaatt ttatcaaaaa
     2401 ggtagtcttt atgaaaaaag aaaacaatta ttttcctaat tttataaaga aaaagagaat
     2461 aagctgcaag cctttcaggc tgagggctta tcctcttttc gactaatcta cataatacat
     2521 gattgcaaac gcgtctttac cagtatgcgt agcgataacc ggattcgtat gcagtacagg
     2581 gatatgcatg tccgggaata attcttgcaa accctctttg aattcttgcg ccaattcttt
     2641 tgcatccgca tgtgaaatac cgatttgtcg cacatttggc aattctttca gttcattttt
     2701 caaattatta aaccatttat cgaacgtttt ctttcctcgc cccttagtta cagggattaa
     2761 ttcagaatgg gcgaaattca tcaccacgcg tatgttcaaa acactagaca gcaaaccagt
     2821 tgttcggctg attcttccac cttttaccaa gttatctagt gtggatattc cgatgtataa
     2881 ttttgtattg tcttgtactt ttttgacttc ttcaaggatt ttctctactg ttttaccttc
     2941 ttttgctaat tcagctgctc gaattacctg aaaagataat ccttggtcag tggtatcact
     3001 gtcgataacc gttactttcg tttttgttag attactggct tgtcttgctg cttctactgt
     3061 tccactcaag ccttttgtca tatggatcga aatcacttca ctgccgtctt ctcctagctc
     3121 atcatacatc gagatgaatt ctccaattgg tggttggctt gttttgggca gattttttgc
     3181 tttcgccatc atttccataa atctttcgcc gtctagttgg tcatcatcgg gatatactgt
     3241 tccgtcgatc atgatggata gcggcatgac acgaatgcct aaagcatcgc gtacattttt
     3301 ttccatcgta catgaagaat ctgttacgat ttttacattt gtcattattt tctctctctc
     3361 tttctttaac aagatgcctt attcatgtta aactacatgt aagacaattt ctatttttag
     3421 tataacagac aaataatgat ttttcattaa ttacacctag taaaggagcc gttttcatgg
     3481 aaaaaacgaa atttaatcga acgtatgaaa tattaaatga agtgtttaat gcagtcaccc
     3541 atggaattgg gactggattg agtatcgcgg gactagtggt tcttttagta aaaggtgccc
     3601 acttgcattc ctccctccat attgtctctt atgctatcta tggatcgatg atgattcttt
     3661 tatttttaac gtctactttg tttcattcat tgatttttac aaaagcaaaa aaggtgttcc
     3721 aagtattcga tcatagttcg atttttcttt tgattgcagg aagttatact cctttttgtt
     3781 tgctgagtat ccgaggatgg ctaggttgga ctttatttgt attaatctgg ttgtttgcga
     3841 ttcttggcat tatatataaa gcgttgacgt tgcataaacg ggagagagta tcaaaagtat
     3901 ctacgatcat ctatattttt atggggtggc tctgtgtcgt ggctgctgtt cctctttatc
     3961 actctcttgg aatgactggt gttattttga tggtagcagg cgggctttct tacacgatag
     4021 gcgcattctt ttatagcttg aattctatcc gctatatgca tgttgtttgg catttgtttg
     4081 ttatgttagg tgcaggtttt atgtacttct ctgttttatt ctttacttaa taaaaaaaat
     4141 aaagctatgg atgggacagc tctaaataac agctagtcta tccatagctt ttttatatcg
     4201 tcaattcgcc tgtcccgctt tttacaggat tctcattacg ctgatacgtt tcaaattgat
     4261 gcggataacg atttttttca tccatttctc cttgactagt attgatcagt gaccaatcag
     4321 accagtcaac aggtggaaaa taagcatctc catcgaatac atgatggatg actgtacggt
     4381 ataatatcgt acaaaatggc agaaattctt gataaacagc tgaccctcca gcgatgaaaa
     4441 agataccgtc tgtttctttt tccagagcaa gcacgtcttc cacactatgc atgacatgca
     4501 cgccttctgc tttataggaa gaatctctcg tcaaaacaat cgtctgacga ttcggcaacg
     4561 gacggctacc catcccttcg aatgtttttc gtcccatcac tagtgtattt gcttctgtca
     4621 tttgtttgaa gaatttcaga tcatttggca aacgccaagg aagacggtct tcttttccaa
     4681 tcaatccgtt ttcatcctgt gcccaaattg cagctaacat tttctcatcc tttctgattc
     4741 taaacagcga taggagcttt gatcgccgga tgtgggtcat atccttctaa ttggatatct
     4801 tccatctcaa aatcaaatac tgactgcttt tcctgattca aaactaaagt cggaaatgcg
     4861 cgtatttctc ggctcaattg ttctttcatt tgttcgatat gattgatata aagatgagca
     4921 tctcctaatg tgtgaacaaa ttcgccaacc gctaaacctg tttcatgtgc aattaggtga
     4981 gtcagcaacg cataacttgc aatgttgaac ggaacgccta gaaacacatc cccactgcgt
     5041 tgatacaatt ggcagcttag ccgtccctct tgaacataaa attgaaacat cgtatgacaa
     5101 ggaggcaaag ccatgctagg aacatcttcc ggattccatg cagaaacgat caatcttcta
     5161 gaatcaggag tctttttgat tgcttcgatc acttgtttga tttgatcgat aaagccaccg
     5221 tcttttgttt cccagtgacg ccattgatag ccgtagatgt gtccaagatc gccgtatgtt
     5281 tcagcaaagg aatcatcggt taggatcttc tcgcaaaaaa gcttgctctg ctttttatat
     5341 tcttcattga attcaggatc tgtcaaacat ctatggccaa aatcagtcat atctggtcca
     5401 ttatagtctg gacttttgat gtagcgttca aatgcccatt catcccaaat atggttttta
     5461 ttttgtaata gatagcggat attcgtatct ccttttaaaa accaaagcaa ttcgcttttg
     5521 atcaatccaa aaggtactcg tttcgttgtt aacaaaggaa accctttttg cagatcaaac
     5581 cgcatttggt atccgaataa gcttaatgtg cctgtgcctg ttctgtcttc tttacgatgc
     5641 ccttcttcca acaattttct tcctaagtca agatacgctt gttccatcct ttttcctcct
     5701 aatcttctac ccattcactc aagtattccc accgtgccat tttttcttca agttgtgctt
     5761 ctgtttcaga tacgtcgttt tgcaattctt gtaatcgagt gaaattatct ccttgatgat
     5821 tcatctcttc ttggagcaat gagatttttt cttcaagttc tgcaatctca tcttcaatcg
     5881 tttcccattc tttttgttcc atgtaagata atttcttctt ttcagcaggt tccttgctct
     5941 gtatttttgg tttcgctttt tcagcaggct gctgggtctc tttttgcttc agtaaataac
     6001 tggacatgtt tccgtaataa agttcttgtt ttccgtttcc ttggaatact aataacttat
     6061 ctgctacctt gtccaagaaa taacggtcat gggaaacagt gatcacagca cctggaaaac
     6121 ttctaaaata atcttccaaa atcgtcaatg ttgctatgtc caaatcattg gtcggttcat
     6181 ccaataataa aacattaggt tgctgaatca gcagtttcaa caaaaataac cggcgttttt
     6241 ctccgcctga aagtttgcct atcagtgtcc catgcataaa tcttggaaat aaaaatcgtt
     6301 caagtaattc tgctactccg atttgactgc catctgctgt ttttgcttct tcagcagctt
     6361 cttgtaagta agcaatcatc cgtttatctg gtgccatttc ttcattttct tgtgtataat
     6421 acgcaagatg aaccgtttca ccgatagaca ttgtaccgct atccagggga attctgccag
     6481 ctagaatatt aagcaatgtc gattttcccg caccattttt accagtaata cctagtcttt
     6541 cccgagattg aatcaataaa tctaaatgtt ctagcagtgt ttggtcattt atacggtaac
     6601 tgccatcttt gatttccagt acttttttcc ctaaacgttg tgtcgctagg ttcagctcta
     6661 aatcatcttc ttggtttact tggtacaagt tctcctttag atcttcgaaa cggttgatcc
     6721 gtgcttgctg cttggtgcca cgtgcctgta cgccagccct catccaagct aattcctgtt
     6781 tataaagccg cttgcgcttt tcttcttgtt ctacttctac acgatcacgt tctgcttttt
     6841 ctaagacgta agcttcataa tttcctttat actcgtataa ttttccaaat gaaagttcga
     6901 agattcggtt tgctactcta tctagaaaat aacgatcgtg ggtaaccatc aatagtgcgc
     6961 cacggtattg tttcaaatag ttttccaacc actcgatcgc ttcataatct aaatggtttg
     7021 ttggttcgtc tagtaatagt aaatcgggtt catcaatcaa cacttgcgcc agactaactc
     7081 gttttttctg accacctgat aaagtactga tttttttatc taattcactg attcctaatt
     7141 tttgcaaaat aatctttgcg ttggtatctg tagtccaagc atcttcttta ttcatttgtt
     7201 cttctgcttt agcataacgt ttttgcacat cagggtcttc tccgttttcc cctagttcga
     7261 tcaatgcttc ctcatatgct cgaacagttt ggatcaaggg gctgtttcct tgaaagactg
     7321 cttggagaac agttaattca tcggaaaatt cagctgcttg agaaagataa ccaatacggt
     7381 aatcagttgg ataaaacact gtttgccgat caccgtcgct gctatcgata ccggcaataa
     7441 tatttaagag acttgtttta cccgtcccgt tcgtcccaat caacccgata cggtcttttt
     7501 cgtggaccaa aaaagaaatc tggtcaaaca aatctttttc accataagtt tttttgaggt
     7561 cagttacttt taattctttc aacatgttcc ctccttatct tggatattgt atcataaaaa
     7621 tagtgaactt caggaaaatt tttttggtat ttgttctttt tgtgttctca aaaaaagaac
     7681 aggaccgatt cgatcctgtt ctgatatgtt ttgtttttta taaaacttga aaatttttac
     7741 ctttttattt tattggactc gttttttttg actttgctcg aaacgcaaat cggaaagaaa
     7801 agttatcaga tggtttgttc atcgtataag attctaacaa attgatattc ctgcgatagc
     7861 tgttcgcaga aaacgtcgtg attttttcag caacttcgta ttcatcaatg atttttcttt
     7921 caagataata acctcttagc atttttttcg taaacagact atcttgctgg atcatccacg
     7981 aagaaatcaa attccccgag ttcattttta ttccttcaag tttgcgttgc tggataaaaa
     8041 attgaaccat cgtttcatca taaatatcag tcaagttatc taaactttca ataatcaact
     8101 gagtattttt ttgatatgtc tctctgattt cttggatatc ttcatcacta ataaatgtct
     8161 gacgatcagc atatcttctt tgccaaaaca ttttcggatg gagaacgatt cgcattaacc
     8221 tacggacaac aatgaaccaa aagccgataa aagacaaaat ctggcttgtg atagaatgtt
     8281 cgaatcgaga aagaaaacgg gagtaaaaac gataaccatt agaagaaagc ttgcccgaac
     8341 gataactttc atccaatcca tcccgttcga tcgacaaaat cagtgcttgt atttcttgta
     8401 cttcttgctt ttcatcatcg gttaaatctt ccaagtacag atcacgtatc cgttcttgat
     8461 aattttctat cactgcttca gtggctaaaa attcttttgt attacgatca atttccttta
     8521 tttcttttcg caaaacttca acgacctctt ctaaaatcat caaagcattc aaatccgtgc
     8581 ttttcgcttc ctctgtttcc gtcagcatag gcaagacaat gatccctatg accaaagtca
     8641 ctaagatgac acccgccgtc agaaacaaaa gcaaagaccg ctggtaaaac atcatattat
     8701 tgattgaaaa tggcaaaata aaaatcgtag ccaaactgac tgttcccttt acaccgccaa
     8761 atgttaagat caagatctcg ttcaactgtt ttttgaactt tttactgcca tctttgaata
     8821 cataaaaaag actgatagaa atgaatctga tcacaaaaag catgacagag attaatacga
     8881 taatcgctaa taaaagaccg tttggatata aaccgtctcc ccatactggc gagaacactt
     8941 gcgtcaattc gattcctaaa aatataaata ctaatgcatt cagtgtaaaa gcaatagttg
     9001 tccatgtact gtgggacaag ctcgacaatt ctgcatcaaa cacagagatt ttcctaaaac
     9061 cagaagcttg gagaatacct gctgccactg cagcaatgat tccagataca tcaaatgcct
     9121 ctgccaatac ataggctaga aaaggaagca aaagttcgat cagcagatac cctgtgacat
     9181 cttgtgctga tgctttttcg atcaaatgaa ttattttttg tttgaaccag acaagcaaaa
     9241 agccgatcaa agctccccca atactagaaa ctactagact catccccgcg tttactgctg
     9301 aaaaagatcc ggtgatcagc gcaccaagag caaactggaa ggcagtaaca ccagatgcat
     9361 cattcaaaag accttctcct tccaaaatat gcatcgcctt tttagggata ttcactcgtc
     9421 cagaaagaga actgactgct accgcatctg tcggacctaa cgctgctcca aaagcaaagc
     9481 aggcagcaag tgggacactt ggcaacagga atgataacgt cgcacctacc ccgacaaggg
     9541 tcaaaatgac tcctccaaat gccaagaaca aaattgtccc gaaatttttg agaatcgaag
     9601 aaatatctgc tttttctcct tctctaaata acaatggtgc aataatcata actaagaaca
     9661 attcaggttc aaaatcgatt gattgtcccc actctgtcag acctaatacc actccaagga
     9721 aaatttggat cagtggagct ggaatcattg gcaaaatgcg gctagctatg ttcgataagg
     9781 tgatcgttac tgcaaataca ataactaact cagcaaattc catcgtccat cccctttatt
     9841 ttttattttg tgtaagcatt tttaaaaatt gttctttttt ctttaaaatt ttggtttcga
     9901 ctacttcaac ctgtgtttgg ttcccctcta attcacactg tacacgtttt gactccaaac
     9961 gaagcagatc atgtttcaga cgttccaact gacgatacat caatatattt cttttacgca
    10021 acttacaaag tatttgacgg cggtcatcca tcgaaagggc gccatagcga tcatgcaaca
    10081 gttttttttg ttgttcaaaa ctctctctca ttttccagat ctcctataca caaacttcca
    10141 tcattatagc aaagttcttt ttggatgaaa aatatttcat tgtttcatcg tttattgcta
    10201 gttagaaatt tccataaatt tttcaacgcc tcattctatt ccaaatagta tcagatatca
    10261 agtaaaactt aagaaagata aaagtttctg acaaaaggat gtttactttc ccattcctgc
    10321 ctttcttgtt cttttatcac aacaaaaaaa tggggctgcg acaaaaatct tgtcacagtc
    10381 ccttattctg aataaacggt gtggaagaga cagcctatca gtagtaataa gacacatctc
    10441 tttcacagcc tcatttttgc gtaaaaatcg atttattcga aaaattcccc gtatacatct
    10501 tctacacgtt gatctggatt gatcacgata tacaagttac ctgttttttc gctgattttt
    10561 gttgattgat attgattgtc taatccttct gttcctttat ctagatatgg gaagtacaca
    10621 agatcaggtg tgccatttcg gtaaaggatt tccattcgct cgatatcttc gtatttcaat
    10681 gcgcgattaa acatgcccat ttccagtccg cctaggttga tatcatgtgt cgcaactgcg
    10741 tccccttcat ggaagatttc gattttaaat ccagcacacg gatggatttc aacaaattca
    10801 ctgccattga ttcgtccaaa acttgttgtg atttgtttga tccaaagatc gccgatatga
    10861 cggcggttga tcgtccaggt ttctccattt ctaaaataaa attttagcgc tgtcatttct
    10921 ttactcatta aaaagactcc tttatgatgt aatttattct tgtactacct cagtttagca
    10981 caaatttatg taagtggtca catcttatgt tttttcttct aatgacgtat ctgcccactt
    11041 taagtagcca acaaagctga gcagatacag tctagtgttt tttaaatcaa tttttctttc
    11101 gataaaaaat ccaaaacctg ttttttatca ttttttattc tttgttctac gacggctttt
    11161 tcagctaatt gcaaagcttc actaacccac ttaccaggtt tttttccagt ttcttctagc
    11221 aagattttac catctactgc taattcgctt aatgacttga taggtaaacg atcataggat
    11281 tctcctacgg catccatctg aggtgtttcg tcaaaataga tcatcaattc ttctacttgc
    11341 agtgcatgtt ttttgcctaa gaagtacaat tcgaatggtt ccaaagctcc agtttctcgt
    11401 tgtcgtagtc cttggacaag tgcttgaacg tctttaatca tttggttaga acacttccac
    11461 gatttcaaaa aggaacgaat ctcattttct tttagatcca gttggctgat caataatgcc
    11521 catgcttggc tctcttcttt caatgggacg gatggtaatt cagcaaaacg taaaagtgct
    11581 tcgccttctt ctttcaaacc gggacagcaa atgtagcact ctgtttcaat aaatgcatgg
    11641 attcccgcgg cccgttcatt tcccaagagc atcttgatga attccacatt tatacgttct
    11701 acagatattt tacctaatag ctcgtggttt tctttgatgg cgtagaaggt ttcttcctct
    11761 aatttgaacc ctagttgact gacaaaacgc aacccgcgca tcattcgtaa ggcatcttca
    11821 tggaaccgct catgaggatt gcctacagct cgcaagatct gttcttctag atcttgcaga
    11881 ccatcgaaca aatcgatgat ttctccatct tctttcaaag caaacgcatt gatcgtaaaa
    11941 tctcttcttt tcaaatcttc tgccaacgaa cgaacgaatt cgacatgatc tggtctacgg
    12001 tagtcctgat aagttgattc cgtccgaaat gtggtgatct catattgttc atcattttct
    12061 aataccaaca cagttccatg ttcgatccca atatctatcg tttttggaaa aagctgtttg
    12121 atttcttctg gaaatgcgct tgttgcgata tccacatcat ggattggctg gttcagcaag
    12181 atatcacgaa cacttccacc tacaaaataa gcttcatatc ctgcattctc taattttttc
    12241 agaactggaa ttgccttttg gtattccaga ggtaattgtt ttaatttcat ttttccaacc
    12301 tttcctgcgt gtttcttgtc ctgagataaa gactcagttg catggtgact gtttgacaag
    12361 tccgtttcag agtcttcaag gccaggacca cttcatcttg tttactcgtc cttacgctca
    12421 aagcgattat ggtctctttg attgaatttt tccatgtttt tttcaaaaac ttcagttaag
    12481 tcgatattca atgaattagc catgatcatc gtaacgaata aaacatcacc aagttcttct
    12541 gctaccgagt taactggttc agaagccttt ttgctttttt ctccataagt gtggttgact
    12601 tctcttgcca attcacctac ttcttcagtt aatcgagcca tctgagctaa tggagagaaa
    12661 taccccacct tgaattgctg gatatattca tccacttcct gctgcatact gtataaagaa
    12721 cgctttttat ccatcttttt ccctccaaaa ctatcttacc aaaactttaa cgcaaatcct
    12781 agataagagc aattgagaaa atcgaatttc catgctacat ttaggaagga atttctaaat
    12841 ggaaattctg tgaaaataca taattgaaag ctggaattca tatggtcaaa cgaaaaacag
    12901 taaaagaagt tctttttatc attataggaa caagtattta tgcatttggt cttgtttact
    12961 tgaacatcgc taatcattta gcagaagggg gagtctcagg gatcaccttg atcttaagag
    13021 ctttatttgg tattgatcca gcttatacga ctttattaat caacatccca ttgattttac
    13081 taggaggaaa gatcctcggt aaacgttcct tggcctatac tgttctaggt acactttctt
    13141 tgtcattctt tttatggatc tggcaaaggg ttccactaga aatcaattta cagcatgatc
    13201 tactgatcgt ttctctactt gcgggtttag ttgcaggtgt aggaagcggg atcgtctacc
    13261 gcatgggtgg aacaacaggc ggcagtgata ttatcgctcg gattttagaa caaaattacg
    13321 gcatcactat ggggcgttcc ctattggcat ttgatatcat cgtcttactt gcttcattaa
    13381 cctatatcga cttgaaacgt atgatgtata cgttgattgt ttcgtatgtt ttcagtcgtg
    13441 tgattgattc gatcttagat ggcggatatt ctgcaaaagg tctacttgtc gtctccaaca
    13501 aaagcgaaga gattgcacca ctactgatga ccggtctgca aagaggtgtg actttccttt
    13561 ctggagaagg cgcattctct ggtaattcaa aaaaaatcat ctatatggtc gttagctcta
    13621 gtgaattaac tgaagctaag aggatcgtcc acgagattga cgaaaaagct ttcctttcaa
    13681 tcatcaatgt gcatgaagtg gaagggcaag gctttactta tttgaagcca cagacaagat
    13741 tactgaaaaa actataagaa aaaagacaaa gataggctat gaggaaagat tccacatcca
    13801 gctgaccttt gtctttttta gtactatact atttttaact aattgttatg ttcacttacc
    13861 cggttacttt tcttttgtgt ctgcaaaagg atctttcggt taacttcatc agataatttc
    13921 aaaaaagcat ccctatctcc atcttctagt gcttgatcga tttgagcata aagcattttt
    13981 agttttgcct cttcttcttt ttcttgaaaa tatcgttcaa ttaattcagt atcttcttca
    14041 ctgactgaag aatcccaaga agaaaatgga ttgtcttcta agatagatag atattctctt
    14101 gtctgccaag cattttcgaa tatacattcc acatataaag gctttttcca attcaaacgg
    14161 atttcatgaa aaatctggtc tgtgtccgta aattcttttc cctgcaaaaa caatttcatg
    14221 ggttcccctt caacagatag atcactgatc tgcagtcctc gatccgcaaa attcgcccct
    14281 tcaacaaagt ggacattgct taaaatcgct tcatgattgg caagataatt caagatccaa
    14341 aaaacttctc ttcggctaaa agatacatga ttgaccatcc acgttaaaaa attcttcttt
    14401 tcccgtacat caattagcat ttttctcacc tctctgttag gtcatctaat aatgacagtg
    14461 cctccatatc tcctggttca agtgttaaat aataagtcaa taattcttgc gctcttttca
    14521 gctgcccttc ttctcttaag aaaaaggcat actctttcaa gaaatctggt tcatgtgata
    14581 gttcatgata agcctgttca tagtgtacag ctgcaacggt aaagtcttct aattcattat
    14641 aagcatgggc taggttccat tctgcataag gattagcagt ttcttccatc tgattgatca
    14701 cttgtatcac atcttcatat ctttcatcat ccaagtaaag attacttaaa gtaagtaatg
    14761 tttcatcttg tttgtctcct acttctaaag ctttcagtaa aagttcttct gctcgctgtc
    14821 tatcatgcag acggaaagca ttttccgagg ccaggtgata aagttcgact tggtaaggat
    14881 tttctttgat tccttcttct aacactgttt gtgcttcttc aatcatttct tcttcttgaa
    14941 gagcttgtcc tagatataga tataacgatt gataatgcgg attaacttct cgaagttctt
    15001 ccagatagtt gattgcctgc tgattttctt tcaattgaag attcgtaaaa gctagctgga
    15061 acagtcgatc atctgtcctt tcttctttca gtgcttcttc taaaggtacg atagctgctt
    15121 cgaattctcc ttgcatactt aaggattgtc ctaaacgttc ctgcatcgag attcctgaaa
    15181 tttcatttat cccgcttgcc aacaatgttt cataaaccac tgctgcctct ttgaaacgat
    15241 caactgaaaa atacaattca gctagcgcaa actggatcaa tggttcatca ggaagcaacc
    15301 cagctgcttc tttcaatttt gcttcactca cttcagggat tccgataact tggtacaaat
    15361 cagctatcgc caataagctt tgcggatagt aatcactttg cttatccact ttttccaggt
    15421 attcaaaagc taagtcgatc tcattatctt cgattgcaat ttctgctaac gggatgttca
    15481 gtccgtcatt ttctgggtat tggttgatca gttgttcaaa aatctgtttt gcttcgatca
    15541 aaaagccaat agccagcaat tcttcgccta gttcagctaa tatatctgct gggtcttctt
    15601 ttaatgcttt ttctaaaaag atttgtgctt gtgacaagtc ttcatctgat aaagcttgca
    15661 acattttttc gctatttgta ttcatttcaa ttccctctta tctcactgac gatttgttga
    15721 aataccgtca ttgttctcaa taattctttt tcgtttggat gttggtatcc gtctattata
    15781 cctatttttt ctaaaacaag tatttttttg cttttttctg ccaaaaaaag aactttttcc
    15841 aaatactcca tctttgaaat ctgttccagc cagtggattg gatagccaag caagtccagc
    15901 cacacaaaaa agttttttag ttggaaatca aaaggatatg cttggagatt ccacacgaaa
    15961 tgaaacatca ttcctaaaaa ttttttcata ctagcagaaa gtagatggcc attttcggtc
    16021 agataaaaag cttgttcaaa cagtttacca taatttgaca ggttttctgc ctctaattga
    16081 taaaaataga tcatttcttc gatcatcccg gcaaacggcc gagtcgttaa ttgcttttta
    16141 tttggatagt ttttatacag attttgcaaa aaacgatgat cacaaatcat tccgcaacag
    16201 ataaaagtca aaaagtcaat ctgctttccg tccacctgcc gctcgctgat tgtttgatca
    16261 taaacgatgc gttctggcag attgatggtt tgcagcacta cacgatttgg tagctgcaaa
    16321 atcgtccgct gcggtgttaa tgctcggctt agagaacgga tcgatacagg gatcatccat
    16381 agtgatgaat caagggttgt atgtttttga aaaaaacctg ccagatccat gatcccttca
    16441 tttccaaatc cgatgataaa catggattga ttttcgggat agcgtttgac gaaatcaagc
    16501 aggttcttta attctgttaa atgattgcat tgagtgttgg cacaaatata ccagtcattg
    16561 tttgcttgat ttgcaaataa cgggttgatc ttatctgcaa acaaatcata atagcgctga
    16621 ttcgttaaaa ataagattgg ctgatcaatg gaaagttctc ttagctgact gactagcgtt
    16681 tctccgtaaa acactttcgt caacgcatct tctacttgat aatcaagttc catacgcatc
    16741 cctcctcttc ttatttttat tttaccatta gtaagcaaat tctagaaaag ataagagcaa
    16801 agctcacctc ataacttgca aataaataaa aattaaataa aatacatgtg ttttctatgt
    16861 atgacatacg gattcggaat ctctgtctct tatttattcc ttttctgcta ggaaataaaa
    16921 agaaaagcac ttgtagccct agagccacaa gcgctttttc aactgtctta tttaacagcg
    16981 tcttttaatg ctttacctgg tttgaatgca ggtactttgc tagctggaat ttcgatttct
    17041 tcaccagttt gtgggttacg acctttgcga gctgcgcgtt cgcgaacttc aaagttacca
    17101 aaaccgatta attgaacttt ttcgccgtta gctaaagcat cttgaatagt tgaaaataca
    17161 gcatctactg ctgcagttgc atctttttta gttaaatcag tagctgaagc aactttttcg
    17221 attaattctg ctttgtttgc catggataga ttcacctcct ccacaagaga tatgatagaa
    17281 atatcattta gttaaaactt tctgagtggt ccagaagttt aaaaagaaat attgattaac
    17341 ttcaatattc tgttatcacg atatcatatg aaccgcttaa tagcaaggtt tttaccaatt
    17401 ttcacatgct ttttttagtc atagaaacgt aacaaacgaa actgtctttt tcttttgaaa
    17461 aaaaaacgtc gatatggtaa aatgcctatt ttctcctacg agggataatt cggatggacg
    17521 tcccttcaaa agtaaatgct tttctgattt gattttctaa gaaacgttca taagaaaaat
    17581 gcatcaattc ctcttcattg acaaaaacga caaatgtagg tggtttgaca gcgacttgtg
    17641 tagcataaaa aatcttcagg cgttttcctt tatctgttgg tgttggattg attgctacag
    17701 catccataat gatatcattc aacacagccg aagagatacg aagattctgg ttcatactta
    17761 cttgttcgat catttcaggc agacgttcca accgttgttt agtcactgca gaaacaaaaa
    17821 cgattggagc gtaatcaaga taccggaatt catcacgaat ctcttgttcg aaatcacgca
    17881 ttgtattggt ttctttcttt acaagatccc atttattcac gacgataata attccacgac
    17941 cagcttcatg ggcgtaaccc gctactcgct tgtcttgttc ccgaatccct tcttcagcat
    18001 tcagtaccat caaaacgata tcagaacggt caatggcacg cattgcacgc atcacgctgt
    18061 atttttcagt attttcgtat acttttccgc gtttacgcat accagctgta tctatcatta
    18121 agaatttctg accactttca gaaacgaagt gggtgtcgat tgcgtctcgg gtcgttcctt
    18181 caatatccga aacaattaca cgttcttctc ctaaaatcgc attgatcaaa gaggatttac
    18241 caacatttgg gcgaccaatc aaactaaact taatcgtgtc gtcgtcctct tcttcagtct
    18301 cttcagagaa atacttcact gcttcatcta aaatatcacc aatccctagt ccatggcttc
    18361 ctgaaacagg ataaggatcg cctagcccta aggaataaaa ttcatagata tcgtttctca
    18421 tttctggatt atctacttta ttgactgcta aaatcactgg tttattactt ctgtataaaa
    18481 ttttcgcaac catctcatct gcgtctgtca cgccttcgcg tccgctgact acacaaatga
    18541 ttacatctgc ttcttcaatc gcgatttctg cttgatgctt gatctgatcc ataaaaggtt
    18601 catcgccaag atcaattccg cctgtgtcga tcacgctgaa ttctcgacct aaccattcac
    18661 cttttgcata aatacgatca cgagttactc caggagtatc ttcgacaatc gagatacgct
    18721 caccagcgat gcggttaaaa atcgtagact taccgacgtt cgggcggcca acgattgcaa
    18781 ttgttggatt tgccatatta tttcctcctc acaagtttcc aatctcactt agtttaacga
    18841 atgcattgcc aactttcaag catagattgt caattaaacg aagtattttt attccttatt
    18901 gaaatactaa tcgcttttca aaagaaaaac tgagacaaaa gcagctgctt tcatctcagt
    18961 cacagataca aaatcaatta tttttcagaa tcatcggttg ctttttcttc tacttcttct
    19021 ttcaacgcat cacctaaaat gtctcccata gtaaatccag tattttcttc tggtaattca
    19081 taagactcaa cattttttgg ttcttcttct gaagctggtt tttcttctaa agctttgata
    19141 ctcaatgcaa tacgatgttc ttctggatgg atctctaata ctttcacttt cacttcgtca
    19201 ccttcatgaa gcacttcatg tggtgtcgca atatgtttat gtgaaatttg agagatatgg
    19261 actagccctt ctactccagg gaacacttca acaaaggcac cgaagcttgt caaacgttta
    19321 actgttccgt ctaatactgt gccaactgga gcttttgtat cgatttctga ccaaggtcca
    19381 ggctgtgtgt ctttgattga caaggaaaca cgttcttgtt caggattgat agacaacact
    19441 ttgacattta catcatcatt gacagccaat acatcacttg gttttgctac atgactatgt
    19501 gaaatctctg agacatggac tagaccgtca attccgccta ggtcgacaaa tgcgccaaaa
    19561 tcagtaagac gtgcaacacg tccactgaga acatctccct catggatagt tgaaagaatt
    19621 tcttttttct tttcttctct ttcgttttcc actaccgctt tatgcgataa gatcaaacga
    19681 ttttctgatg gttcgatttc gatgatttta aagctcaatg ttttgccttt atagtctgaa
    19741 aagtctgcaa caaaatgatc ttccaccatt gatgcaggga caaaaccacg tacaccgacg
    19801 tcaacgacta aaccgccttt gacaacattt gtaacaggtg cttcgatgac cttgccctct
    19861 tggaaatctc tttcgatatc ttcccaaact tttttagcat ctaaacggcg ttttgaaagt
    19921 agatagctgc cgttttcttt atctttgccg atcgtactga taacgactaa atctaataca
    19981 tcaccgactt ttactacttc gctgatatct tctaccggca aagttgataa ttcttttgcc
    20041 ggaacgactc cttcaactcc agccccttcg attccgacaa tcacttgttt gtcttcaatg
    20101 actaaaactt cgcccttaac aacatcgcca acttttactt cttgtacgct tttcattgcg
    20161 tcttccattg actggttgtt ctcttgattt tgatcaaatt ctgtcataac acttgtcctc
    20221 ctaaccaaca ttctgccgta agtacggtac tgtttttagt ttaattgatt attcaaaaaa
    20281 aataaagcga tttcgttcgt tttccttgaa aaaacaattg tttttcattt tttttcttaa
    20341 tttaaaaaag aagataacct tttttcaaaa caacagcttt tattgctgca acgacttctg
    20401 gaatcgattt gccagtcgta tcgatacgaa cagcatcctc tgcttgcttc agcggagaga
    20461 cgtcccttgt tgaatcataa tggtctcttt cctgtatctc tttcgtcagt gtttcaagat
    20521 cagtcggaat tcctttttct tggttctctt tgaaacgtct ttctgcacgc tcacttacgc
    20581 ttgctactaa aaagattttc acttctgcat tcggtaatac agctgtcccg atgtctcttc
    20641 cgtccatgac aacgccaccg cgttccccta tttttcgttg gacagcaact aattcttctc
    20701 tgacttttcc atgtgcagag acttcagaaa cagcttttgt gacatctggt tgacggattt
    20761 ctaaagtcac atcttttgat gctatgaata catgctgtcc atcttcttgt tgcgaaaaag
    20821 agataggata ctttttgatc agcgaaacta gcccttcttc atctgaaaaa gatacattgt
    20881 tcgtaatagc taaataagtt actgcgcgat acatggcacc ggtatctgta taaatataat
    20941 tcaattcttt tgctaatatc ttggcaacgg tacttttccc agatgaagca ggcccgtcga
    21001 ttgcaatact gatttgtgtc atgatataac tcctttttct cagaaaaaaa gccgcaaagc
    21061 gcggctatta ttatttaatt cttaattctt gaccagggta aagcataaag ttgtttggat
    21121 ctaagccatt caattcaaac aattgatcag tggtgatccc tgcacgctca gccacttgtt
    21181 ttggaccttc gcctgcttga acagtcgttg ttgaaccggc tgcagctgat gaagaaggtg
    21241 ttgtttctgc tgcattgctg cttgaatccc ctgcttgata agttgcctca cttgaggaag
    21301 tcggtgttgt ctctgctgca atactgcttt ctgacgttac acctgtatct tgagacacag
    21361 tagattcttc cgcgattgta ctagattcta ctgttgaagt ctcagttgac tcctcttttg
    21421 ttgtacttgt agaactttct actactgatg tcgaagtttc agaagaagtt gctgctgttg
    21481 cattgttgct tgatccattt ctgatccaga aataagcacc tgctggaata gcgatacata
    21541 agaccagtaa aatcaaaaat agcgaaagaa ataatgtatt tcctttcttt tgttgtctct
    21601 gagaacttct tgatgaaact tcatcatctg tatcgtagat tggttgttcc catggttctt
    21661 gtgtttcgtt attttctgta gattgatgtc tatcttttct gctcactgtt ttacctccta
    21721 aactctatcg tgtgtattat accataggaa aaagtgtctt gtcctaaaaa atcaatgttc
    21781 catgtttttt ttaaataaat ttagtaaaat cgtttcccaa tgaagttgtt cagtcttaga
    21841 aaaaagcttc ctctttggca gaagttctgc ttttgctcca tcaatatcac aacaattctg
    21901 cggcttttcg gagagttttt cgccaaaata agctaagatg aattttctgc ggcagttttc
    21961 ttcattaata tagcgaagca tctgctgcag gcggaattct ttttcttttt cctgacgttt
    22021 gagccgttcc acccaatttt ctggtttttc agactgctgg atcagctcca tccatttttt
    22081 ttgcagctca tcaaagggag cttgctctgt ggcatattcc aaataaagct caaaactttg
    22141 tcgctgttct ctagaaagtt gattaaagaa ataatggata cgttcatccc cactttgata
    22201 aaaaagaatt gctgcacttt cttcctgatc tctgcctgct cgaccgattt cttgtacata
    22261 gttttctaga ctgtctggca aatcataatg gaccacataa cggatatcag atttatcgat
    22321 ccccatacca aatgcattcg tagcaatgag aaactgcagc tgatttttta caaactgctg
    22381 ttgaagttgt cttctttgag ctgcatcaag accaccgtga tagtatccaa ctgtaaaacg
    22441 ttccctgaat aaatgataca atcgttcgac ttcttttttt gttgcacaat aaatgattgc
    22501 cgctccttgt gctctttcca tgaagtgttc aagttcttgt tctttttgct gcgttttccg
    22561 aacaaaaagc gagatatttt gccgattgac tgattgtctg atctcttttg gtggtcgatc
    22621 aaatagtaca tgacgtatat ctcttgctac taaatctgtt gctgtcgccg ttagtgccag
    22681 tgtgaccggg tttcctaaaa gctttcggat ttttcctaac tgttgatatt ctggacggaa
    22741 atcaactccc cattgagaca cgcaatgagc ctcatctact acatataacg caatattttg
    22801 ttttttcaga tgctctaaaa gagaaggatt agtcaacatt tcgggactca aaaacaaaaa
    22861 tttatattgc gatagatgac ggaggacgta tcttctttca gattgcgaaa gtaaactatt
    22921 gaaagcagct actcgtttct cccctcgttt ttgtaattgc gtcacttgat cttccattag
    22981 agaaagcagc ggtgacacga tgatgaccag accttccatt aaataccccg tcaattgata
    23041 gcaaaggctt tttcccgttc ctgtaggcaa aatagcgaga gtatcttctc catcaagcaa
    23101 cgcctgaata atttcttctt ggccgggacg aaatgaatca aatccaaaat atttttttaa
    23161 ttcatttatt aacattctgt atcgcttcct ttctcaatag atagatttga tacaaacgaa
    23221 attctccata atctaattgt ccatctacat tccattcgct aaagcgccac tctcgcacgg
    23281 aatccttatt tggttccaaa cgttcgattg tttctgcaga gagaatccac tcgtatggaa
    23341 aatcatcaaa aaacaaggcc cactcaataa gatgatctgt tactgttcct ctttttaaat
    23401 gacggattgc catgatctgg tcaattgtct gaccattcat aaacatctgc ttggtgatca
    23461 tcatgctttg attgaaattt tgctgtaata atgaatcgat caaagcgtgc caaagcgaat
    23521 ctggccgttt ttcgatttgt gctaacaaaa ggtggataca tctgctttgt tgcagataaa
    23581 ccgctgactc atcattgttt tttgccagct gataaggaag cagccctgtt cgctcatttc
    23641 cagataattg gttagctaaa aaatctgctg cttcttctgg tatctccgaa aacagctgca
    23701 ccaaactctc atacgcagta tcaatcaaaa tttcacgtgg caattggctt tcactaagcc
    23761 atttttttag ttggaaagta taaaaaggac ttgtctctgc tggaagatag tcttgaatac
    23821 ccgttgccag attagaaatt acttgaacag caaatttaat gagccgccaa ctagtttccc
    23881 ctgttctgcc atagcggtca aaacgcaaac cagtatgttc tatttgatgt tcggacaaca
    23941 tatcagcccc tgcaggcgtt agctttgctt cattttcatt tatttcgatc caaccttgct
    24001 gcgcaatttt ttgcaaaatt tgataaaact tatcctgctt caattctgga aaactgttat
    24061 ggataaacaa aagttcgtgg gtaaatccat aaataagtac tgaagtcgtt cgttttccca
    24121 caaggagttg gtaaagagaa ctaatccgca gcttgtgtcc tgatgaaaat agcgataaaa
    24181 taaaaacaat catattattc tccttaagtt aggatggtta gaaatgtcac tactatgtaa
    24241 acttgtacct gaacgctgta ttgcatgcgg tctttgtcaa atcgaagcgc cagatatttt
    24301 cgattatgat gaagaaggaa tcgttttatt tgcccaagaa ccagaggctc atcaacagtt
    24361 cgtgatgccg gaagaagaaa catatgtaaa aaaagcgtat caaaaatgtc cagttcgagc
    24421 gattttatta gaaaataaaa tataaatata aacgaaactt taaaaacgta cactaagttc
    24481 tctaaacttg aaaataagcc gggatctgtt tctcttgttc cgtgaaatcc cgacttattt
    24541 tgaaagattc atttgctatc tatagttaat tgactagttt tttcacggac cctataggac
    24601 ccatttatat tatttcaact ttttcagcca gttttccaat gtattgacga tcacagtttg
    24661 ctggtctgcg ttagaaattt ctgcctctcc atctcctttt tgctcaccat agctgccaaa
    24721 acctccatga tttccgccat tgattgatac atactctgtt ctttccggta aatactgctt
    24781 agcttcttga taagcttcct gattcaatac tttatctttt gtcgccgtga tcgataaaac
    24841 aggaacagct gattctgtca atgttccttt tttgtcggga taacttgcca tgaagaatac
    24901 cccttttaaa tgtttgtctg aatgattctt attgacataa cgacttgcca ttacgccacc
    24961 taatgaatgg ccaccgatca cataatcgga atttggaaaa tcttctttga ccttatccgc
    25021 tttgtttccg cccatcactg ctagatcaag tggcattttg acaatagcga ctgaaaagcc
    25081 gttttttgct aaatcttgcg cccaaatact ataactttct ggctctacga gagcaccagg
    25141 ataaaaaata agtaatggag actttccatg attctttttc tctccttcaa aaaataagta
    25201 atcttctttg ttttttcctt cttttaccaa cacttgcgct tctgacgatg gttcgtacgt
    25261 attttgctgt atatagtaat aaccggctcc accgagtaat cctaaaatga tgactaagcc
    25321 aattagtatt cgtctaaata ttttcacttt ctcccacctt ttctatctga gtgacccctt
    25381 tttcttaatc aagtatgatt ataccttaaa aaaataagag agaaatgcaa actttccagc
    25441 atcaaataaa aaaagacgga atatccattt gaatatccgc cctttttgta tgtttatttt
    25501 tatttagcac ataaacgata cacagcttct gcataaattt ccattgcttt atacatacta
    25561 cttaattccc atcgttcatt cgcctggtgc ataaagtcag gagtatcagg gaacatcgca
    25621 ccgaatgcca cacactggtt catcgtacga gcaaaagtag cgccgcctga tacaaacggt
    25681 tcagtcatgt ctccagtaat atcgcgatat gtccccaata gatttttcac taactcactg
    25741 tctagtggca cgtataatga atcaaggaag tcaaattctt cataagtcaa cccatatttt
    25801 tctgctgttt tagttaattt agctactaaa tcatctttct tgaatgtaac agggattctc
    25861 atatctacac cgatttttgt ttgttctggt gtgatttcca aacttgcaaa gttcatcgtt
    25921 agctcgccgg attgttcatc ttctgttttg cccacgacag cttcaccagt tgcattttct
    25981 tgaactaatt ttcctaagaa attgattggg ccaaaatcaa atacttctga aagagcaatt
    26041 gccagacgag aaatagcatt cactccttgt gctgcatctt tcgcgtggat acttttacca
    26101 agaacgacga tgccttcacc ttgttcttcg aaatcgaatt cgtgtttttt caatgcttct
    26161 tttacttcag aaagcttttc tccagagtaa ggagctgcat caggaacaac attcaacgct
    26221 cctccagctt ttacagaaaa ctcgttcgtt cctggtcctg tcaagtaagc ttgcaataag
    26281 cctttttctg cataaattaa aggaaattca gcatctggcg caaatccttg cgtaattcct
    26341 tcttcttttt cgttatattt ttctaaacaa cgccataaat tttcttcatc tgtaccaaag
    26401 ataaaacgaa tacgtgtctt gaattccacg ccagcatcaa tcaaagcttt gacggcatac
    26461 attgccgcaa tagaaggacc tttgtcatct tgggatccgc gaccaactaa ccagccgtct
    26521 tttatagttg gatcgaatgg ttttgtttcc caattatttt cgtcccctgc tggaacaacg
    26581 tccatatgac acaagatacc gaataattca tctccagagc caatctctga ataaccgtaa
    26641 tagccttctg gatctttaaa tgttctaaat cctagctttt cactgatttc caatacttta
    26701 tccaaagctt caatcacttt tttaccgaag ggatgtcctt gtcctgaatc tgcttcatct
    26761 agtacggaag gaattcggat caattcgctc aatgattcta atgcttgttc atgatgttgt
    26821 tctgtcacaa attttttcat gatattatcc tcctaatatg ttaaaaaggg tcatgtcaaa
    26881 ttcctccgac acaacctctt cttctcaaat aatgacgttt taagcctgct ccatcagcaa
    26941 gcagaatgtt cttaacaaat gatgaaagaa aaaacactca ctgaatggag ctattgactt
    27001 atccacgtgc ttatcgatag ccgtttatcg gtttcttcat tcttatgata gaccttgtac
    27061 ctgtatttgt ctaggtttag aacattgctg ctacacctag gatcacacag atggctacaa
    27121 ataatatcgc aattaattta ccggtaaatt tccaccacac tgaaacatcg aaacgagcaa
    27181 ttgctacagc acccattacg atagcagatg ttggtgtaat taagtttact aaaccagatg
    27241 cagattgata agcagtgatt actaaatcct taccgacgcc ggcaaattca cccataggtc
    27301 ccatgattcc cattgttgca gcagctaagc cggaagttga aggaatcaag aatgacatag
    27361 ggatatagaa aataaatgta ataatgatga atacacttga agataatgaa cttaatcctt
    27421 gttcgcccca atgtaagatg gtgtctgtga ttaatccatt gttcataacc acttggatac
    27481 cacgagcaac agctactacg attgctacac cgatcaaatc agaagcacca gctaaaaatt
    27541 cagagacaaa ttcagactct ttcatgccat aagtgattgc tactacgatg gacattacta
    27601 agaataacat agtaatttct gggaagtacc agtcacctaa tggaagcatg cttttaccta
    27661 gaaccgtacc tagtaccgga aggtttgtta accagtttgt caatgattca aaaatcgtga
    27721 aattcgcatt caaggttgtc caaggaatca aactgatgat catgatacca aaagtaataa
    27781 agaacaatac attaacatgt ttttgttttt tcgtgattac ttcttgacga ccgattgctt
    27841 caatatcgaa atgcttcata tcttctttac gttggttgta tactaatgat tttgttggat
    27901 ctttttcgat ctttgatgca taacgataca catatacaat agaaatagct aacaaaataa
    27961 cgaacatcaa gaatcgaaga ccaatacctt cacccatcga aatcccaaca gcttgtgaag
    28021 ctacgcctgt tgcaaaagga tttaccgttg aagctagaca gcctacttga gaccctacta
    28081 atacgattgc tacagctact actgtatcaa agcctacagc catcatgact gggatcagta
    28141 aagcatagaa tggaatcgtt tcttctgcca ttccgtaagt agagccgcct aatgcaaata
    28201 acagcatcaa tactggaata agcatttttt cttttccttt gaatttcttc acaactgaag
    28261 caatcccggc atctagtgcc cctgttttgt tgataatacc taaaaatcca ccaacaacca
    28321 aaatgaagaa agagattggg attgcagcag gtgtttcttt agttagctcc cccgctggta
    28381 cacctagcat cccattgatt ggcgccatga aaatatccca cagcccttga ggattttgtt
    28441 gtactcgttc atatgatcca gcgacgatgt tgcctgcatc gtctacacta tagtgcccag
    28501 ctggtataaa ccaagtaagt acagcgatta ctgcaataat aataaataag accgtatacg
    28561 ctgaaggcat ttgaaatttt ttcttttttg tttgttccat cttcttcttc ctttcttttc
    28621 cgttagagac aacaattttg gctctaatgt tattttatca aaaagagtat cagaacaaaa
    28681 ataataacac gcattttgta aaacttttgt tcaaaaagtt cgtttttccc tattccctcc
    28741 tcttttttca ggtttttaaa gctttcttct ttttcattgt agcgtttcct tttttttata
    28801 aacaaacatt ttcgtctttt aaaaggagaa gaatttcaca tttgcaaaga attgattgat
    28861 ttttatgcat aaatca
//
LOCUS       DAJSMI010000015        27611 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJSMI010000015 DAJSMI010000000
VERSION     DAJSMI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.015"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(185..1129)
                     /locus_tag="ON430_000578"
     CDS             complement(185..1129)
                     /locus_tag="ON430_000578"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR3218522.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1425..2369)
                     /locus_tag="ON430_000579"
     CDS             complement(1425..2369)
                     /locus_tag="ON430_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3218523.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2590..3804
                     /locus_tag="ON430_000580"
     CDS             2590..3804
                     /locus_tag="ON430_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3218524.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3826..5841
                     /locus_tag="ON430_000581"
     CDS             3826..5841
                     /locus_tag="ON430_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCR3218525.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5924..7297)
                     /locus_tag="ON430_000582"
     CDS             complement(5924..7297)
                     /locus_tag="ON430_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR3218526.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7323..7607)
                     /locus_tag="ON430_000583"
     CDS             complement(7323..7607)
                     /locus_tag="ON430_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3218527.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7609..9624)
                     /locus_tag="ON430_000584"
     CDS             complement(7609..9624)
                     /locus_tag="ON430_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3218528.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9803..10108)
                     /locus_tag="ON430_000585"
     CDS             complement(9803..10108)
                     /locus_tag="ON430_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCR3218529.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10197..11228)
                     /locus_tag="ON430_000586"
     CDS             complement(10197..11228)
                     /locus_tag="ON430_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3218530.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11435..12310
                     /locus_tag="ON430_000587"
     CDS             11435..12310
                     /locus_tag="ON430_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3218531.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12462..13304)
                     /locus_tag="ON430_000588"
     CDS             complement(12462..13304)
                     /locus_tag="ON430_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3218532.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13396..15273)
                     /locus_tag="ON430_000589"
     CDS             complement(13396..15273)
                     /locus_tag="ON430_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3218533.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     RPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEATIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIDNQTNTPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDHETSGTYAYVLYDG
                     DKEIDPTNYSVTEQTNGFTVSVDSNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15270..16691)
                     /locus_tag="ON430_000590"
     CDS             complement(15270..16691)
                     /locus_tag="ON430_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3218534.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQAATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQTEIAKNGA
                     SSGTFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKAPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGISTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16694..20083)
                     /locus_tag="ON430_000591"
     CDS             complement(16694..20083)
                     /locus_tag="ON430_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR3218535.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDCVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNSWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKNLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20340..21731
                     /locus_tag="ON430_000592"
     CDS             20340..21731
                     /locus_tag="ON430_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3218536.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21804..22451)
                     /locus_tag="ON430_000593"
     CDS             complement(21804..22451)
                     /locus_tag="ON430_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR3218537.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22459..23145)
                     /locus_tag="ON430_000594"
     CDS             complement(22459..23145)
                     /locus_tag="ON430_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218538.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23151..24224)
                     /locus_tag="ON430_000595"
     CDS             complement(23151..24224)
                     /locus_tag="ON430_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218539.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24446..25120
                     /locus_tag="ON430_000596"
     CDS             24446..25120
                     /locus_tag="ON430_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3218540.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQPPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25125..26465
                     /locus_tag="ON430_000597"
     CDS             25125..26465
                     /locus_tag="ON430_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3218541.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMATIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26614..27600)
                     /locus_tag="ON430_000598"
     CDS             complement(26614..27600)
                     /locus_tag="ON430_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCR3218542.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDENLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8030 a         5238 c         5022 g         9321 t
ORIGIN      
        1 cgattcgctt attgtaatca acagattaaa tttcatatgt tcatatgtac ggatacattt
       61 tcggaccgat taattttaac cattctgttt atttgcttac ctattagata tcaataaaaa
      121 actgcttaac agctatttca gctgttaagc agttttagga tttttagttc atattcattg
      181 tttcttataa tacagtgttc aatacttctc ttaaagcact tgttgatttg ctgaacattg
      241 ctttttcttc ttcgttgatt gataattcaa caacttcacg aacaccattt tcgtcaacga
      301 ttgatggcac acctgtaaag atatcttttt cgtcgtattc accagttaaa taagcagata
      361 caggcaatac agcttgttcg ttattcaaga tagcttttac gatacgtgca gtactcatac
      421 caataccata gtaagttgct tttttacgat caatgatctc ataagcagca ttacggactt
      481 tatcagcaat cacatctaat tcatcttggg caatacgatg gtctttttct acaatttcaa
      541 acactggttt accaccgaca gttgtatgtg accatgcagc aacttctgaa tcaccatgtt
      601 cacctaagat atagccgtgg acgctgcgtg ggtcaacttt taatttcaat gcgatttctt
      661 tacggaaacg agttgtatcc aatgttgttc cagtaccgat aacacgtgat gttggcagac
      721 cagattcgtt ccaagcgata tatgtcaaaa tatctactgg gtttgaagca acaacgataa
      781 taccgtcgaa tcctgatccc atgatttctt ttacgatttg gcgcatgatt gacgcatttg
      841 ttttaactaa atctaaacga gattgaccag gtttttggtt gataccagct gtgataacaa
      901 cgatatcagc atctttacat tcttcatagc cacctgacca tacagccaca ttttcatcgc
      961 cccaagccat accatctaaa aggtccaatg cttccccttc tgctttttct tgattcacat
     1021 cgatcaatac taattcatta gagattcctt ggttgatcat tgcataagcg atacttgttc
     1081 caacaaaacc tgtaccgacg attactactt tacgacttgt ttttttcatg aataaacagc
     1141 tccttttcat aactatttgt tactttcatg aaaagtatag tcttgtttat tcattttatc
     1201 aacatgttaa ttataatagt tgttattata atttttgatg gaaacgaatt cctgaatacg
     1261 cataacagat aaatgacaaa tgtttacacc tatatcccct gataatacaa ccaaaagttt
     1321 agaactgaaa ataaatccaa aaaaattcgc tgcaacgata taataagaca gcaatcgcac
     1381 ctaaaacaat tttccttcat atgctttagt gaaagatttt cttcttaatg tttatttctc
     1441 cattcattgg gagtacatgt tgaatacttc ttgaataatt tgaataaaga agtcgtttgt
     1501 ttatatccga tacgattagc gatttcttcc aacgaaaaac tcgtttcttg aatcagttcc
     1561 aacgcaaagt cgaatttttt ctttagaacc agttcttgaa aggaagcatc aaaatacatt
     1621 ttcaatttat tactaagata gaccggattg tagccaaaat gagctgctgt ttcttttaac
     1681 gtacaagaag taaaatgctt atcgatataa tctaatactc taagcatctc attttctgcg
     1741 catccatctg ctgatgaaaa gctttgttgt tctaataaat gagtcaattc gatcagtaat
     1801 agtgatgtat agagatttaa cgaacggatc ttatgactgt atgtatcaaa ataaatcaac
     1861 agcatatttt caatcaaatg cgtcaaacga cttgtttttc cgccgcgaaa atgaatgaat
     1921 tgtgtatgtt cccctttttc ttttgatgca tttatcaaaa aagaagaaag tagattattt
     1981 gtgcttgcca aatgattcaa tatctctgta gataaggttt cttcttttaa taagatgttg
     2041 attaaaatat cctcttgccc tgtgtattcg attctttgag tagcttcttt atcaatgatc
     2101 accacgtcac tttcttccat ctctatcctt tctccattaa taatttgtaa acatgtaccg
     2161 ctatacatat aattgatttc aacaaaatca tgtgtatgct cgggaacttc cgcatatcga
     2221 cggtgtttac tgatatgaat atttccctgt tgaaatagat cccctcggtg gatttttggc
     2281 ggtgtctgaa gatggtaagc taagtttaaa tcattgatat tcattccttg ttcacgctgc
     2341 agagtttcga ttgattcttc gttacgcaaa aaaaatgtct aattcagcta gttccaattg
     2401 actccctcct ttttgtaagc ggtttctttc gcttattagt tcacgctgta ctgctattat
     2461 atgcaaagct atttttggtt tcataaagac tttctatggt tgggacatga acttcctgtg
     2521 attccgctat tgagagaata accacttcat actatatttt tgataagcgc ttacaaaaat
     2581 gaaaggaaca tgcttatgaa taaaaagatg atgaaatttt ctctgctgtc agtctctctt
     2641 attttgactt ctgctggtgc agtcacgtct gcaattccca gtatgtcagc tacttatgcc
     2701 acgcgttctt tatctgctgt tgaattatta acgactgctc cctcttttat ggtcactatt
     2761 tttgttttga ttagtagttt tattgctagg aaaatcggac aaaaacaaac tgtgatcatc
     2821 ggattagtga ttgcatcgat ttgtggtatc ctccccctct tctcacaaag ttacagtatt
     2881 gtcttagctt caagagctgg actaggtatt ggctttggat tgatcaattc tttggcagtc
     2941 agcatgattg ctgcctttta tcacggagat gaacgagcct ctatgattgg ttttcaaagt
     3001 gcatttcaag gatttggtgc agctttaatg acatttgttg caggacagct agtacagttc
     3061 ggttggcaat ataccttttt agtctacgcc atcagcttac ctattcttgt cttgtttgtt
     3121 ttgtttgttc ctacacctga aacaactgaa cttcaatcgc atggcacagc cccaacaaga
     3181 caagtaatca aaacgaatcc taaaatgatt agttatggta tttttctctt gctggtcatc
     3241 gtggtatata atgctgtatc gattaaatta gccacagtcc tgacgactta ttctctagga
     3301 aacgaaacta atgcagcgaa tatcttaaca atcatgcaat tggcaagtat ggctgccgga
     3361 gtttgttttg gctggcttca gacaaaagca catcgctata tgcttacact ttctttacta
     3421 ctgatgggaa tcggctttgt attgattgcc actgtgataa acctgtatgt gattgggttt
     3481 ggtgcgatcc taacaggaat ttctttttcg ttattcattc cttacttatt caaccaagta
     3541 agtatccaag cgcccaagta cgcggagagt ttcaatactt caatcttatt ggttggcgct
     3601 aatttaggta ctttcattgc accatatggt ttgctctttc tctcttttgc ttctcttggg
     3661 agtaaaacac ttgctgtttt cgtcaatggc agtatcttgc tgttgattag cgcgcttatt
     3721 agccttctag tagtacacag gacgaataga aagacagtga taagagtaga tgaagaaata
     3781 aaagaacaat tgacaacaaa ataaatttga tggaggtcgt gtaaaatgac aaatgaaaaa
     3841 cataaagata gatggctttg gtatcctgga gactttgaga tccgccatgg actgctacaa
     3901 aatttccaac gggaagaacg aggttttgac tggccagcgt actggtacat ggatgattgc
     3961 catcggaacg tcaagtttaa gagatattac tttcttgatc aaccttccat gtttaaagtg
     4021 acgatccaag gagttgggta tgtagaaatt aatggacaaa aacatccatg tggaaaatgg
     4081 ctgacttgtc cagctggtaa agcaaaaatt cgtatctttg taggacatac atcagggcta
     4141 ccagcgatgt ttatcgaggg agacgaagta aaatcagata ttggctggac agcaagtaat
     4201 tttattgaag aatatccagc tggctggagt ccgctttatg tcgatatcgc aaaagatcca
     4261 aatcaaatct attatcaaaa agaatgcgtc catccagtca ctgaaacgga gattggcgga
     4321 ggaatcttat tcgattttgg gcgagcagtg aatggctgtg tagaactaat atctctggat
     4381 gaccatatcg aatctatcac tctatgttat ggagagtccg atgtagaagc tatggatatc
     4441 gattattgct actataagca aataggaatg acaagaaaaa tgaaagctcg taaacgcgct
     4501 tttcgctatt tgtttatacc agaggtttct actggaatgg ttcaacttca agtttttcat
     4561 gaatttttat ctctttcgaa acaagctgct ttttcaagcg attcttcttt actgaatcag
     4621 atctggtcag tatcagaaga agccttcaag ttatgtagtg atttgttttt tatcgacggt
     4681 atcaaacgag atcgctggat ttggtctgga gatgcgtatc aatcattcat gatcaatcaa
     4741 tatctttttt ttgacgagga aatcaataaa cgaacgatat tagcattaag aggacaaaat
     4801 gaaatcaagc agcatatgaa tactatcgtt gactattcga tcttgtggtt gattggtatc
     4861 gaaaaccact atatgatgac gaaagactat gaatttttac gcatcgtcta ccctaagatg
     4921 caatcattaa tgagatactt gatggagcaa acgaatgaat tagggtttat ttatgggaga
     4981 gcaaaagatt ggatatttat tgattggtct gaaatggata aagaaggaac gcttgctgca
     5041 gaacaagtct tattactgaa agcctatcat tccatgatca cttgcggaga tatcttagct
     5101 gaaacatatg gagagaacga aaattatcat actcatctct atcaagaaaa atatcaacta
     5161 ttgaaacaaa acctatttga ctacttttgg gatgaagaac ttggcgcttt tatcgattgc
     5221 tatgaatctg ggagaagaaa tgtgacgcgt catgcgaata ttttcgctgt tttattcgat
     5281 tttgctaaca aagaccaaca gcaatcgatc ttaaagaatg tcttattgaa tgacactatc
     5341 acacagatta ctactccata tttcaaattc tatgaacaag atgctttatg caaactcgga
     5401 cagacgaata tcgtctacca aacgattctt gattattggg gagggatgtt atctcatgat
     5461 gcggtaactt tctgggaaga atacgaccct actcaaacag gaaatgaaaa gtacagtatg
     5521 tatggagatc cttatggaaa aagcttatgc catgcatggg gagcaagccc aatctatctt
     5581 ttaggacgct attttatcgg tttgtatcca acaaagccag gttatgaaga atttatgata
     5641 caacctaatc tagattcatt tgaaacactg aattgtacgc tacctatcaa agatggaaag
     5701 atttcgcttg aaaaaacaaa agataagatc gtgattactt ctgatagaac aggcggaaca
     5761 gtgaaaatga atggtaaaag ttatcctctt ctagcaaaac agccacttgt cttaacaagc
     5821 tattcaaaaa taaatgcata aaacaaaaaa ctgcttgcga catatcagaa gactttaaat
     5881 cttcttctga tattgtccaa gcaattttta ttttgaagtt tgcttaattc tgttctttag
     5941 aaacaatgtt tgttcttttt ccggcaaaaa gaaagctaga taatacgaca agtaaaaaga
     6001 aagcgatgat tccaactgtc aaggctattt gaccgtattc tcctagtttc ccaagtacaa
     6061 gtcctggtac tagataatca gtatcggcaa atgtagaaga agcgatttta agatctccta
     6121 atactggaag gataaataat ggcagccaac taatcaataa gctattgaaa gcagaaccta
     6181 ataccgcccc tcttctaccg ccagtcgagt tgccgaagac accagaagca gctccgcaga
     6241 agaaatgtcc tactacccca ggaatgataa ctgtagtttg caagcctatc atagcaaaca
     6301 tggatagtgt tcctacaaag aagctaacaa aaaagccaat taatacagca ttaggtgcat
     6361 atggaaaaac gataggaaca tctaatgctg gtttagaatc gggtacgagt tttttagcaa
     6421 tcccttggaa tgctggaacg atttctgcca acaccattcg aaccccttga aggacgataa
     6481 caaacccagc tgtgaatgtt ccagcttgga tcagtgcaaa gattacagcg ttgtctccat
     6541 tgcttagttc tctttcaaca aatccaggac cagctaacaa agcaagtgcg acatatacga
     6601 tcgacatcaa gagcatgatc gatacagtag aatctcgcaa aaagctgaaa cttttaggaa
     6661 tctttatgtc ctctgtagaa cggtctttgt ttccaaaaaa ctttccgatc catccactag
     6721 aagcataacc gatatttccg gtatgcccta acgcaacttg attgtttcca gtgatgttct
     6781 tcataaacgg ttgacaaaga gcaggcgtaa tagttaatag tgttccttcg aagaccccgc
     6841 ctaataagat catccacaaa tttgcagtaa accccgcgct tactaaaatg actgctgtta
     6901 gacaagaaac ataaagcatt gcttgacctg taagaaaaat gaatttatat cttgtgaatc
     6961 tagcaagtaa aatattcacg atcatcccta ctagcatgat caaagcagta ggtgtaccgt
     7021 attgttgcaa agcaatcgcc actactgctt cattgctagg aactactcct tgagtatgca
     7081 gagctgtttg gaacatcgaa gcaaaagggg ttagtgaatt actgattaaa ccagcaccgc
     7141 cgatcagtac taggaatccg acaaatgttt tgatagttcc ttggatcact tctgaaaatt
     7201 tcttcttctg aagaacgaga ccaagcagtg caatcaaaga aacgaggatg gagggctgac
     7261 tgacgattga cacaaacaaa ttcaacaatt tatccattga tagttcctcc cttgaatcat
     7321 ttttattctt ttgacaagat cttcgttact ttttctttta cttcttcgct gtcaatgatc
     7381 gattgcaaag gtacgagctt ttcatcaggt atagaagtta gcgtatcttt caaagaatgc
     7441 tcaatgaaga aataatcagc catatcgcta gatacacttc caacatccgt atgacttact
     7501 tcaaactcgt ccacatctgc tccaagatct gcaagtgctt gtttgatgtt catctctacc
     7561 ataaaacttg ttcctaatcc actttgacat actgcaaaaa ttttcatatc attcgtctcc
     7621 ttttcttatg atcgctaata tttcttcttt tgttgatgcg tgtaataaat cttgaagatt
     7681 ttgtttatta gaaagtagct ttgttaaatt tgccaaagcc cgaagatgtg cctcattatc
     7741 gatagcagct aaacagatga acaaactaat gggatgggct ggattatcaa gcagatccac
     7801 aggttgttga agtttcagca aagacatccc tacttcgttt acgccatctt ctggacgagc
     7861 atgaggtaat gcgatgtgat cgccaatatg gataaacgca ccatactgct ccacgcgctc
     7921 gatcatagct ttgatataac tttcttctat tttattttct gataatagcg gctgtgcggc
     7981 aaatgtgatt gcttcttgcc agttttttgg ttgatccgat aattggatca tttcttctgt
     8041 taataattca gatagcatag gacgttgatc ctccttcttt tctaaaagtt tgttcatttt
     8101 acgatttaat acttgataga gcttttcttc gtctacgcct tcttttaatt cgatataagg
     8161 cagtagcgcc gacaacaatt cttgggcaga aggcattgaa aatcctggta tcagccaatc
     8221 tctttgtact tgattgatca gctgattttt ttccaagtca gtcatgattg gatgcagaac
     8281 atagagagac tttttcttat cagtaagtgt gagaggtaca gtagaaaaaa taatatcgta
     8341 agaagtttcg tcaacctcgt gtaagcggtc agctgaatta gtctctgtaa aaaagatcat
     8401 cgggaataat tgctgaagtt cactttttaa gatcaatgaa gagctaatgc cacttggaca
     8461 gacgatcacg gctcttattt ttctatttct ttcttctgcg tccgcttttc tgatctctcc
     8521 gccaaatagt atagtgaaaa aaccaatctc ttcttcaggg atgctcttcc ctattcgtct
     8581 ttctaacgga agtaatgctt ttcttgtcat ttcgtagatg gaagcatatt gatgcttaat
     8641 ttgatcgatc atcacattag gtaaataaaa agaataggaa atacgaaaat aggcagggac
     8701 caagtgataa aagacattca tcaataattc acgaaacgaa gcaaattcta cggccatcag
     8761 gaattccatg cgatggatga cttctgaaga acattccagc aaaaaatcaa gtgcaggctc
     8821 ctggacatct ccttcaacga tcgatgctaa gcaaagagag aaaaatataa tctccgaatt
     8881 ttgatgttcg gaatagccaa ggccagtcaa tacttgtcga ctaatgtcag tgactgcaag
     8941 agaagccaac agttcagcat cttttaccag ttccatggat gtttttccaa ttcgaatggt
     9001 tgtcaaactc aaataataag ctaattcatc cattcgattt ggtgcgaatc gaagatctga
     9061 tagtgaaatc gttttatcta gcaaatctct aactttggcg taataatcaa tggaaagggt
     9121 tgcggttaaa tacgtgagcc caaatcttcc agcaagtgac tgcatcaaac gagcagtgcg
     9181 attttttgct tctcttctta atattgcttc tgaccctagc agcctaaaac cagttttcct
     9241 agtatacgac agctgacaat cttccacttc taattcattt cttatctttt tgatatctga
     9301 caggactgtc cctttagaaa cctgaagaaa atcttgaaaa tgaaaaacag aaacagacgt
     9361 tgcagtcgaa taaaccatca aataaagaag atctcttcgc tcttgttcgg tatacacagc
     9421 ttcaaatcga gtaatatcca aaaatttttc tgcccagtcg attgctcgga atttctttat
     9481 acggatacgg gtatcatcca catattcggc aaaaggagtt aattgtttga tcgcctcgct
     9541 tgtatttact tcgatgatct ctgctgcttt ttctagactg atttctcttg gtgttttcag
     9601 gagataacta attttgtagt tcacagcgct gttcacctcc tacttatatt ctagtatggt
     9661 agcgctttta aatcgagggg tgtttggtac aacttacagt tggatttttt ttacgactgt
     9721 accgaacgat ttctatcgaa tggatacaga aaaaaagcag gtgaccaaat gtctcctgct
     9781 tttatgaatt gatttcgtcc gatcaatgat gatcatgttt attttttgag gtcagttctt
     9841 caaatttttc tttgacatct tcaaccgctt tttcgatatc tttttcaaag tgattgggct
     9901 cattaggctt tgtttcctct tctacagaat ttgaaccaga taaagctgtt tctttatttt
     9961 ctgtttcacc agagtttgtg ccagctaatg attcttcttt gtcttctgtt tcaccagaat
    10021 tagttcctgc taaagccact tcttcttctg ttttaggggc cattgaacct cttaatcgtt
    10081 tgtgtttttc accgtcataa tttgtcatgt tggttcctcc tagtagttct atactttttc
    10141 atcgtacaat aagacagttt atgcgtcaaa aaacaaaccc gattcttaca aatatgtcat
    10201 atttttcctt ctgattcttg aatgcttttg attccttctt ccaaaatttt catttgtaag
    10261 attgtttctt catctttgat gactttttct gcgccattaa ctaatgtctc gtaaatccct
    10321 tcgtagacac ggctgtaatc accaacctcg gaaattactt tttcttcatg ataactccct
    10381 tgttcatcta tataggtcaa tacaccataa tgttcgggca aatcgatacc aaagtcttca
    10441 tgatcaggca tgtaaaacat tttcagatgt tcttcttgtc gatcttttgt ttgtttcacg
    10501 aacatccctt tttttccgta aacgacgaaa cttggacgtt ctttgatgcg gaaataactg
    10561 gattttatcg aaattttcat atttccatag aaaaaatcca gatcgaagta atcattcatt
    10621 cgtcctttcc ctaaaagttg acgtacatca tagctgatat catctggttc gccgaaataa
    10681 gaaagaactt gatctactgt atgacaagca tgtccatata aatagctgtt cgcagtagaa
    10741 aattcatgcg tattttctgg tacttccggt ctgaaataat cataatgcat ttctacttct
    10801 aaaatatccc ctaattttcc gctttcgatt actttttgga ctgtcaagaa gtcagaatcg
    10861 aaccgtctgt tttggtaaca ttggataaat agaccttttt cttttgctaa tgcaaataac
    10921 tcttctgctt cctttgacgt ttctgcaaaa ggtttctcta ctagtacatt cttaccatgt
    10981 tcgagtgcca tttttccata ttcataatga aattgactag gtgtagtgat gacaactaat
    11041 tggatctcag gatcatccca aagatcagac agctgatcag tatattcgat gtcttcgaat
    11101 gttttccaca ctggttcaga tggggaaaaa atttttttga tattgatttt gttcgtaagt
    11161 tttcttgaaa aaggcaaatg atagcgattt gtactctttc cattgccaag ataagcgatt
    11221 gtaagcatga gtggtgcctc cttttattga tgttttcagt atatcctaga aaagcgcaga
    11281 aagtatttat aatagctatt ctggtaaaaa agttcagaaa aacacttgtt atcattcggt
    11341 ttggtgaaat taatgttcaa agtaaggata aaaatcaaaa agatagtagc ggaaccaagt
    11401 gtttatcagt aagatagaat ttggaggtca gatcatgctt attgaagaaa aactaacaaa
    11461 acaagagttg tttaccacga cagagaaacg gattgtcgat tacatccgta ggaatatcga
    11521 agctgcagtc tacatgacga ttgaagaatt agcaaaagct acgtatactt cccattcagc
    11581 tatcattcgt ttatgcaaaa aaatgggatt cagcggattt aaagaattcc gtttcgagct
    11641 ggggcgggaa gttcatcaat tgattgctca gttcaatcaa acgattgatc ctaattttcc
    11701 tttttcaagt gaagatagca ctcagcaaat tgcaaagaaa atggcggatt tatcgatcca
    11761 gtcaataaaa aaagcacaat tgcagattga acatgaagat ttagatcaaa tcggtaaagt
    11821 attgaacaag gcacagcgaa tctttctttt tgcaaaaggt gattctcaaa tcacggcaag
    11881 gaaattccag aataaaatgg tcaagttgaa taagtttttg atcatggctg aagaatacag
    11941 tgattcttct tggaatgcag ccaatttgac tagcgaggat tgcgccattt ttattagcta
    12001 cagcggtcgc atccatcatt atgaacgaat aatgacctat ttgaagcatg tagaggcccc
    12061 taccttgctg attaccggaa atcagcattc agaaatggcc aaacaggctg atatgtgtct
    12121 tgttatatcg caggaggaat atgattttgc taaagtagcg actttctcct ctcaaattgc
    12181 cttcgattac gtattaaata cgttattctc agtgatatat tctcaaaatt ttgaagagaa
    12241 tatccgcaac ttgaaaaaca aacaagaatt gatgcaaaaa ggccttctta aagaatatcg
    12301 gaaaacttaa gaaaagatcc tcatttagta ataagaaaga tagtgtttga taggctctct
    12361 ttgatatcat cataaaaaca ggctgaagtt ttctttttca aacctcagcc tgtttgttta
    12421 cggaacgatt cgaatagata tattatagaa atgaggaata gctaacttct gtttcttatt
    12481 ttgaacgatt gacgaaaaag atatataaga aggaggaaaa tcagtataca tgcagctaac
    12541 aatccataca ggaaaaatcg tttgttctcg gatactttct gtacttcttt ttgaacttct
    12601 tttgcatgat aaggaacccg gtgccctctg acaagtaagc gatgactgtt gaccatataa
    12661 ggtgtacaag ttaggagtgt cacgaaatcc tgcccttttt cgattctcag atcttttgta
    12721 tcagtcggtt ctaccgtctt gatctgatcg acttgataag cgagtgtccg tccattgatt
    12781 tcgatataga aagaatcacc taaagtcagt ttcggtaagt ctgtgaacaa ctttgcttga
    12841 ggcagtcctc gatggctgga aataacggca tgtgtatttt ctccccccgt aggataagac
    12901 gttccttcca ataatgaggc acctttttct agcagcacag tattcgtttc atcaaaaatt
    12961 ggcaaatgga cattgatctc agggatcgtc aaaataccga tcgtatgttt ctcaaaatag
    13021 gttctatcct ttttcgcagg ttcttttttc ttagtaaagg gatctgcacc tggatttcca
    13081 ccttcttttg ctaattgctg gtttttcttt gtcatgtttt cttggatttt agccatgact
    13141 tcttcatttt ctttgaccgc ttgttgctga taatgagaga tgatctgctg atccagataa
    13201 ttattcagcg cgtcgctaac aaatggataa gctagtatcc ctattcctga gagaagcaag
    13261 aaaagcatca gcagatctag aagtttcctt ttctttggtt tcattcgtgt tctccttgtg
    13321 atgactgttt gataataaaa gaaaaaggag catttgcgta atccgctgct cctttttcca
    13381 attcttgcaa aaagactaaa tctgactgtg cttgcgtcta gcaaagtaca gtccagcaat
    13441 cagtagaagg actactcctg caccgatata cacatagatt cccttaccgc ctgttgaagg
    13501 aagtgtacct ttgtgtttat ttggtatttt ttcaggagaa atcaactgtc ctgctgttac
    13561 ataagattgt tcatcgatag taaatgctac acggtttgtt aatggaacat atttttctgg
    13621 cgctttcgtt tcttccagat agtacgtgcc atattttaga cctgtcacat cgattaaacc
    13681 gttacttgtg gttgtaaaaa ccgttgctga ttctttcgcc gatacccagc tgacggcttt
    13741 tgtggatggg tcgatcgata aatatttcgc tgtgtcacta tcttgatcac gaacgacgaa
    13801 ttctgctcca gcaagtgttt gatctgatgt aacgtcacca tctactttaa caaatcgttt
    13861 gcccccagta acgacatcga ctgacggtgg tgtttgatca tttgtatgcc cgttatcgac
    13921 atttgcttgg ttagaaaatc ctttggttgg atctgctttt tcgttcaaat gcatatagta
    13981 aacaaatttc agcgtaccac caggagtgag tgaaggaata taattcgagt caactgaaac
    14041 cgtgaatccg tttgtttgtt cagtgacaga ataatttgtt ggatcgattt ctttatcccc
    14101 atcatacaag acatacgcat acgttcctga agtttcatga tcaaatgtca aagcggcgtc
    14161 atgagtatca atgagtttga atgtcgtgta cttgttttgt gttccttctt tatcagcgat
    14221 acctaaagga atattgacag agatctcata ttggattttt tctccgattg cgacatcttt
    14281 tccattcaat tgaggtgttg ttttatcaac tttggacgta tcatttttga ttgtcttttc
    14341 tacaggagtt tgactatttg ccaagatctc aaacggcgtg tttgtttgat tatcaatcat
    14401 ttccgcatta tcaggagctt ttacttcttc taaattatat tttccaacct ctaaatgatt
    14461 gacgatcaac tgaccttttt caatcgttgc ctcggcaaag tcattgttgc cgatgtcata
    14521 agaatgacca gtaatgaaat gtttggcttt ggtttgatcc gttgtccaag tgtacaatcc
    14581 atctgtgaca ctttggatgt attttttgac gcttggtctt ccttcttctt tagaaataat
    14641 aaattctgct ccatttagtg cttcgttttc ggcagtaccg atttttgtaa ctttcaacgt
    14701 tccatcatta ccgactgtat ttttagggta gagatggata gtatctagtt cttctgtccc
    14761 gtatttataa gagccatctg cttgtttgat catctcatat acagggaaag ctaaaaccat
    14821 gtttgcggca gctgacactc cgtcttttgg ttcttctttg atcgtgtaga ctgcatcttt
    14881 cccattttgt tttttaggta aagataaagt gacatttcca tcagcatctg tcgttcctga
    14941 agcaactggt gtaccaggag tcaaagactg gactgcttgt tttgctgcat ccacggacgc
    15001 tcctttatct cgttgcgcat aaaattcttg agtgacgtta taaactgaaa atgaaatatc
    15061 ggctaatcct tggtattgat cgaattcgct catttctttc ccgctgtttt ggattaaagg
    15121 atcgggtaaa tcagtcattt tctttttatg aaggatgact tgagctgctg tctcctctgc
    15181 aaacactttt tttgcgccga agctgtttgt gagtaatggt aaaataagga aaaggactcc
    15241 taacataacg tttatttttt tatgattttt cattttttca ctcctgttct ttttttgata
    15301 aacaaccaga ttgcaaagct tgcgaatacc ataccaacaa cgcctaaaag tgtatttttc
    15361 gtttcgtttg tttttggaag aaatggacgg ctattttctt ttttattgac tactttcaat
    15421 atactgtccg cctctttatc ctcagaaaga aaagttgaaa tggtaaacgg aatttctgtt
    15481 tcacttaaaa tataaccaga aggtgcttcg atctctttta atcgataact tcctgttttc
    15541 aacccggaaa ttgaaaaagc gccattttta tcggaaatca ggtgaagcgc atctgattcg
    15601 ttctttgtcc acttgtatcc gttggctgtt tgctggaggt aatttcctgc tttgttttta
    15661 ataaggaata ctgcgccagc taatcgttta tctggtttat ttgtctcgat tttgacaaat
    15721 tgcttactcc ctgtatatac agagactttc tttgtactga taccaaaacc aaaatccagt
    15781 gttccgttgt tgttgatttc cttgttcgcc tgtgctgtac tgcttagctg catctgataa
    15841 gaaatggtca gtttttttcc agcatatttt tccaacgaag ggatcgaaaa taaaacatca
    15901 aatccgtgag gttttttgtg tatgacaaag ccttctgtca gcttttctcc attcgatgaa
    15961 atcgttaaac tattaggtaa aaacgttaat gcttcatccg cactatctga caattcgaac
    16021 ttttgatagt caaggatatt tacaggaatt gtcgtatgca aggaataagt gattttttca
    16081 ccaatcgtaa aatcatttct aggctcttct aataccttct caaaaggtgg atcagggtag
    16141 tcgttttctt catttttagg atacaaatgg atagttgaaa gtttttgatt gttttgtcca
    16201 tgtacaggaa gaacaactac catattctct gccttttctt tgacgacttc tggtgctttg
    16261 gattcaatga aaagatacgc tttatctctt ttttcttgat ctttagcggc cagagaaaaa
    16321 gaagcgatac catcttcgtt gttaagagtt gtagttgttg cttctgcggt aaacgtaccg
    16381 gaagacgcac cgttttttgc gatctctgtt tgtgcttctt ctaccgtttt gcccttttcc
    16441 cgtagatggt aaaaagaatc tgtgacatca taaacttgga atgtgacacc atttaatcct
    16501 cgatacgttt gtaataacgc tttttcttgt ccgtcatttg gatgattttt cggcagttgc
    16561 ccgtttggga aaagcaattt gtgtaaagta atctgaacca tttctgtttc tgtttctgtt
    16621 gctgcctgag taaaagctgg tttaaatagt aacaagaaga gacacatact aagccaacca
    16681 agttttttca tatttagctg ccccctcttt cttttttcaa aaaaagatac caaatagaaa
    16741 acgcacaacc aatcatccct actagataga ttcctaaacg gccaattcct cccgtttcag
    16801 gtaatggtac ttttgcctga ttcgtgatgt ctaaagaaat ctggttgttt ttggctcctg
    16861 gtgacagaac agattcatga tcttgtccat ccacttgaat tgacccatct tcgtgtataa
    16921 ctatagtaac tggctctttt agaccttggt atccttctgg tgtaaaagtt tcggtcagcg
    16981 tataagttcc aggttttaaa ttttcgaata gaaaggtatc tgtttcttgt ccatcttttg
    17041 gcgattctaa gtccatttct ggtccctgca gtctgaattt tgctcctttt aatgtctgtc
    17101 cgttgtcttc ttttttgtgt accgttagat caaacgcttt tagtcgattt tgatgagtga
    17161 gcgtccatac attatttgtc atctcggtat tttttcccgt gtaaatgatt tttccatatt
    17221 tatcgatttg gaattcataa tttcccggcg aagtatcata tccggcagga ccagctgttt
    17281 caacgactcg gtatttacca ggtgtgagtg aatcaaaact ggcaagacct ttttcattcg
    17341 ttgtttggct gtcaattggc tgataagtac catttgcttc ttttttctgt agggaaaatg
    17401 ctgctccttt aagatttatc tctttgtccg tcccttgcat cgcatactta cgtactgcta
    17461 caggaacttc aacaaaagga ttttcaatag tcaactggat cgtatcatcg gaagtagtga
    17521 ctgtttttcc atcaatggtt accgtaccat cagaagcgat cttgatttcc caagtagtct
    17581 ttttgcttaa tccatgccct tctggagctt ttgtttctgt cagcgtatag gtcatttctt
    17641 tttgcaattt gacattttct ggaagagaat aggttccgtc gccatgatct gttagtaaag
    17701 tatcaataga atctcctgtt aatttgaaga cagcaccgat gagattcttt tcacctgtag
    17761 aggaattttt cgttattttc aagtttaacg gtgtgaattg tttagtattt ttccatgtca
    17821 ttgcatctat ttgttgagaa tcgtatcctg gtacaggtaa ttctttgatt gtttggtaac
    17881 tgaatgcttg accttgatta ttgtattgag gtagtgataa tatctcttga taactttctc
    17941 cgctatttgc agataatttg tcaatgtctg cacgttccca cgaatttgta gtatctttag
    18001 ctggtttaat gattcgaata tatccgtttt tccaagctgc agcatttgtc gtatgttccc
    18061 gttgaatctc aaaagtaact tgatctggac gatcagctag attgttgtca aattcttccc
    18121 ataacttttt gatgtgaaga ctgactccgg gagctttagc agatggtata ccgaattcag
    18181 ctaattcatt ggtatcaatg tttggctgga aagttgtcct gccgttcatt tgataccaga
    18241 aatttggatg gaagtcctca ttttctgttt ggattctcac ttggtaatgg atttggattt
    18301 cttggtcttt tcctaaatag atctgattgc tcttgatggt atttccttct atggaaatag
    18361 atggagtgac cgttgtagga cttgtcccca cactcttgac tgaaagtgta ccaggctgat
    18421 aaatgaatgg ttctgcgatt ggatcattta tttgtccatt gcttacagtt gctgagatct
    18481 gtacagcttt tttggctagg tattcagaga tatctgttgc atgatcagct gattcatagt
    18541 aaagatcgcc tttttcatct gatgaaacca tttgtcgcat acgagactct acttctgctt
    18601 ttgagagacc agctgccgga tcgctttgaa gttggatgcc aagaccatgt atttcgattc
    18661 ctcgttcttt gagtgccatc gcttctccga tggttgcgat aaacgtactg tcgattcttc
    18721 tggatagatt gttttggtca ggtgcatcat agattcttga gattagagaa gtatttcccg
    18781 gccgatcttg cgtattagaa aactgagttc cgtaataatt gctgcttgat tgtgcgtgta
    18841 cccgctgtac tttataggaa aatgttggta cacaatccgt cagcaaaacg atcacttttt
    18901 tatgtccatt tggaacggat agcatgcttc ctgcatctct tagtgctttt tgtgtaaaag
    18961 taccaccatt tgtccgtgaa ggtgtaatgg attttacttg atttttcact gaatcaaatg
    19021 aacccatctg tactgcaccg ttactgtagc tatatccttc gcttgagtag ccgacatatc
    19081 ccatattgat tttgtctgtg ataccgctat ctgctaaagt atcgacaaaa cgatcgacac
    19141 caatctttac ttcaccgatc cgattattgt cgttcatact tcctgaccag tctacgacca
    19201 atacgagatc aagaggcgtg atatcttttt gtacatttcc acgagcattc aaataaacat
    19261 caaacaatcc gggtgtactt gtttcctttg catactttcg tatcgcatag tctgcttgat
    19321 taccggttcc tccatattcg atatacgaat gggtccgatc attaggatct ccattccaag
    19381 aatttatacc atcccattga tttgttccgt ttttattgcc ttgatgatca agaacatttg
    19441 tatttcctgt aggttgccaa ctggctttag gataagtgcc ctgatcatct gttgtatatt
    19501 ctgtaggaat cgtttgatta gatggcacac tcacagctgg caaggataat gtaggcaatg
    19561 taaatgggct gacagaattt tcgttttctt ttgatgattc ttcttctatt tcgtctggct
    19621 ttggtactga cacatctgta tctttcttat tttctgtctc atcaataatg tcatattctt
    19681 tattatcgac gatcagcttg tttgcctggc tgtggtaaag tgctggatcg attggaaaac
    19741 tcagatcgat agtttgttta gtgtttgatg agatttccaa cgtaattttc ccgctattta
    19801 cctgataagc atagccatct ccttcttttc tttccacatc acttaagtaa ttttcgacta
    19861 gaccaatttc tttttctatc ttttcagcac tggtgttatt cgcatttagc ttgatgttca
    19921 gtgctttttt tccagactga tccgaaatga cttcagctga aattgtttct aaaactgttg
    19981 gatcatctgt agtgtctgct attgcagata atggcacaaa gtttgataaa ctcaatatta
    20041 tcagcatgaa aataactttc agtttcttcc ctgtggtcgt catttttccc ctcctttttc
    20101 tttttttctg ttttgacgtg aatagtgaaa attattaaaa taaacaggcg ttacataatt
    20161 ttatttattt taatatgata attattatac gaagggcgct aaagggaatc acaccaattt
    20221 tcataccaat tggtttcatt tatgaagttg attccttcga ttaatgagcg tttcattgaa
    20281 agaaaagggt tgagggagaa aaacatatga tcaggttgtt ggaatgaaac tatagaaaga
    20341 tggaaaaaat cattcgaatt ctgtccattt ctgacaagtg gatgactaaa aaagaacttg
    20401 ccaaacgaat tggtagttca gaatctacat ttgtaaggta tatcgaagag atcaaaatcc
    20461 gctggggaac ggatgtaacg attcaatctt cacaaaaatt ggggtatcgt cttgaacatt
    20521 tgaatgtacc agcttattta aatgttctga tggaaatgtc gcaaagttct acaaacagtc
    20581 gtttattaaa tgagattatt gtcaatccgg gtaaaacgat tgagtattat tgtgaaaaaa
    20641 tttccatcag ccggtcttct tttacacgta agttgaaaca atgcaatcaa attttagaaa
    20701 gctatttatt aaaagtgatc gttgaccaag gttttcagtt aactagcctt aaaagtgagc
    20761 tccagttgcg tatctttgtc acctttttct ttttaagtta ctacggaaga cgcacgttgc
    20821 catttccaat cgaaaaaaag aagatcaaag aaattatggt caagaataat tgtcagatga
    20881 atttgtttca ggaagaaagc ccatatgagc aatctttctt tattatgtat tttgttgtgc
    20941 atctcatgag agaatcccaa ggaactccat ttgaatggaa agctggagac tcttatgtac
    21001 agttgataaa tatcgataaa aaagacttct taatcttgaa aaatctctta ggaaaattga
    21061 agtacagcag ctatataaaa gtagtgaact attttgtcaa tacgattttt tcagaatttt
    21121 cggagaaaga aaaaaatgaa atcagtgagc agatacgaaa aaatatgtcc tactgttcgt
    21181 tttcatccgc tgattgcaaa gaaaaaaacg aagaatatgt tgttcaatta ttaacgcaaa
    21241 tttatttttt ctcccgtatt ttcccatacg atacgactca tttgacgatc cgtgcaactt
    21301 actttgcaca gcagatgcaa caccgccaga tacgtctata tgctggattt cgtgcagaac
    21361 ttcaatttta ttccactcta ctgaatcaga atctgattta ttatttatct agctatatct
    21421 tttggatgct tatcggtaat cctgaaattc accattatac ttctgataaa aaaatactga
    21481 ttatcagcga cttaagtctt cgacatagtc aatatattga agaatatatc agtgatattc
    21541 ttgctgtgca taaaattcac tctgagacaa ctgctattac tgaagatcag ctgtcgaaat
    21601 acaatcttct cgagtatgat ctgatcgtta cgaatcaacc cattttgaat gcccatgttc
    21661 ctcatatatt gattgatgat agtgtgtcat tcgcaaatga agaagaactc atccgcttat
    21721 tcgagttatg aataatcaaa aaacagctat gtataaacca ccctgataaa aatttcagga
    21781 caaatacaca tagctgtttc tttttattgt attccttcag caatccaacg agatttatag
    21841 cgaaagtctt ttaacaactc ttcttttgtc caattatttg caatgagatc cgtcaaagtg
    21901 ttcattacga gggtcatgga aaagtatagg aaactaactg cttcttccga cgattgaatg
    21961 gagaaatgat tgattcttaa taattccagc catttttcgt tcattggaga atcagccgat
    22021 agttctctac gtttggttgt ctgctgatca tttcgctggg ttaacaagta taacctttgc
    22081 caatagatac tttcgtgatc taagtcgcag caccactcta aatatttttc gatccccaaa
    22141 aagaaatgtt gtttgttttc gctaatgaat tttaagatat cttgatggac tatgtgagaa
    22201 ctgcttttga tcgtatacaa ataagcatct tccaaatctt gaaaatattt ataaaaagca
    22261 ccgcgcgaca tccccatctt gtcaacgatt tctgagactt tgacttggct gatgtgctgt
    22321 gagcaaaata tctccagtaa taggtcatcg attctctgtt tctttgcgtt cgataaattg
    22381 aaaaaagttt cttttggcat ccacatctct cctttatctc tgattatacg tggatagccc
    22441 ttcattgtca tgaaggattc attccttctt tttctcttct tctgtcaagt acccatcttc
    22501 cattcgataa acgcgatcac tccattgaat catacggaca tcatgagtaa ccataatagt
    22561 cgctttttgt ttctcatgag cttctttaac tagtaatttg acgacttcat atgcatgatc
    22621 tgtatccaaa ctagctgttg gttcgtctgc taagatcaag cttggctcat tatataaagc
    22681 gcgagcaatt gccactctct gtctttctcc accagaaagg tctttaggat aactattttt
    22741 aagtgactgg atatctaaag attgcagcaa ggaatcgatt tttgttgaat cttctttttt
    22801 tgaagtaact tggtcaacta gttcaaattg ttcctttacc gttaaaaaag gaatcaaatt
    22861 agagctttgg agaataaatc ctatttcttt aaagcgtaaa tctgctcgtt ttttttctgg
    22921 aagctcagtt agtgagtgac cgttgatcac tatttctccg gaagtcggtg tttgcaatcc
    22981 tcctgaaatg gtaaggaaag tacttttacc tgaaccagaa ggaccaatga tgctgacgaa
    23041 ttctccttcc tttacataaa aatcaattgc tttcaaagct gagacctttg tatgaccaga
    23101 accaaatgtt ttggtaactt ttttcatcgt taatattttt ttcattttta ttaacctcct
    23161 attgctgtaa ttgggtctac tttggttatc gtacgtatcg aaaacaatcc gcctagcatt
    23221 gcgacaatga tcaaaatccc actatatagc aaccattgtg accaaaaaac agcaaaaggc
    23281 atcgcgcttg gtaaaatcag actagtcaaa taagcaaaca aaagagcaag cagaacgcct
    23341 accaccccaa caataaagga ctgtgctact aaagatttag cgatgaagaa atttttgatc
    23401 ccttgtgctt tcattactcc aaaaatagca gtcttctgaa gtgtcatcac gtacatgaaa
    23461 ataccgacca cggcagcaac cactaagaac aaaaagtata tcatcgcatt taaggtcata
    23521 ttttgggcag aatagccagg aatactttca atgaatgtcc caatagttaa taactgtaga
    23581 cccttcgatg ctttttcttt cgaaatttta gctgaatcct ttattacgat cccattgatc
    23641 ggctgttctt cttcagaagc aaacggctgt tgtccatact tcaaagcagt ccacgtatct
    23701 agggacgcat agatgacggg agaaactgtg taataagttt caggaaagat accgacaatc
    23761 gtcaaaggat cgtcgtaact gccaattttg atcgtatctc caattttata gcctgaatca
    23821 gccagatttt gagagatgat gatttcattc tttgttttaa attcatgccc tttcgtgagt
    23881 tttggtaata aaaatgcttc tttcgttgtg ccgaaaactg taacattttc tttgtttttt
    23941 cctttgattg ctccgctgta caatccgatt ggtgctttct ctcctccttc aatgtatttc
    24001 agatctcctc ttgttaattg tgaagctgca aatacttgat ttgcatcctc cgacaaaaca
    24061 atctcttgtg cgtcccaatc atcgattgct tttttaaact cttctgaaag tccattcgct
    24121 agtcctgaca acatgaatac gacatacgca atcaagaaca taataccgac gatcaagcca
    24181 tatcgtaatt tagaatagcg aatttctttg atagctagaa acatttgttt ttcctcccta
    24241 tgatagattg atagtgacaa aatgtcactt gttgaatagt gtaactctaa agtgacaatt
    24301 tgtcacttgt gttgtttttt tcttatcaat aaattgtcta agacatcttt cgataaaacc
    24361 ttaacatgag tcataaaaaa tttatttaag ccttatttat gctttccatg ataaactaaa
    24421 gtgaccaaat caatgaggtg ggaaaatgat taaactatta gtagtagaag acgataaagt
    24481 attatccaat aatataaaag aaatactcgc tgcaatcggc gagataacac aagtttatga
    24541 tggtagtgaa ggactgttcg agttggaaag tggagtttat gatcttgcaa tcttggattt
    24601 gatgatgccg gaaatgaatg gatatgaagt gttagcaaaa atacggaaag aaaaaataca
    24661 gcctccagta ttgattttga ctgcaaaaga tagtttggaa gataagattg aaggatttga
    24721 acgaggtgca gatgattatc taacaaaacc tttctatcga gaagaactga tcatgcgcgt
    24781 gaaagcatta ttgaaacgct cgctaggtct ctttgatgaa aatgttctga agtataagga
    24841 tatcagttgt gatttaaaaa gcaatgaagt cttgtataaa gaacaagttt tgcccataca
    24901 aggaaaagag tttgaattgc tcgtttattt tttacaaaat aagggaatca ttttgacaaa
    24961 agagcagata tttgaccgga tctggggatt tgattctgat acgacgatta cagttattga
    25021 agtttatatg agcaacttac gaaaacatct gcgaagtact ggattggata aagaaatcaa
    25081 aaccttacgt aatgtcggtt atatactgca aagggtgtga tgcaatgaat ggagatatta
    25141 cacgaaagca acgtagtcgc ttttttgcga caaatgtagc agcatttgct tttatctttt
    25201 ttcttttggg attcattact ttacagatat taaaccaatc tgcatacaga gaaacagatc
    25261 tgtcattaga gaaaataaat tttgattctc gaacagtaca attagagata caaagatatc
    25321 agcaaggcga tcccttttta aacccgactc aaacggaaca aaacagaaca aacatggaac
    25381 agccattaat ggaaccatca aatgaccgtt ttaacactca agtgatcttg tggtctaaaa
    25441 atggtgagat attgaataaa gcagctattg gtgggcgttt caaccaaatc aaagaattaa
    25501 aaatggatac gaaaaatctg aataccatcc aaaatattga attaaatgaa gacagtggct
    25561 catctgccct ctctttccgt tcgattacga aaaaagcgga gacgaatcaa gatggcgttg
    25621 cctacatcca aattttagaa aatacaaatc aaatcaagaa ttcattagcg acttttcgca
    25681 cgattatgat tttgtgtatg attgtttttt ggattctctc tattggtatc agttattact
    25741 tgtctagtct aagcatgcgc cctattattc tttcttggag aagacaaaaa gaatttgttg
    25801 agaatgcttc tcatgaattg cgcacaccgt taacgattat tcaaaatagt ttggaacatc
    25861 tttttaccaa gcctaatcat actattattg aagaatccga aagcatcgct caagcattgt
    25921 cagagacgag gcgcctcact ggattgactt cggatttgct gacaatcgct cggaatgatt
    25981 ctgatcagca gctgctttca aagcaaatga tcaatacaca agaatatatc gaacaacttg
    26041 ttaaaccatt tcaagagatg gccaccatag atggaaaaga gtttattctc cataacgatg
    26101 caacattaga agtaccaatt gatgaaaaaa aaatccatca agttttggtt attttgctgg
    26161 ataatgcatt aaaatatacg aatccagaag ataagatcat tttggattca tctgttaacc
    26221 ataaagaatg gctgattcac gtaaagaata ctggcccttc tatcagtgaa gaagatcaag
    26281 caaggatttt tgatcgattt tatcgggaag atcaatctcg ggcgaaagag actggcggat
    26341 acgggcttgg cttggcaatt gcaaaacagg taatcgaaca gcatcatgga cagatttctg
    26401 tatccaatta tcttccacaa ggagtcgatt ttaaagtcca attgccgttg aagcctaaaa
    26461 aataatgctt atccctaaaa aagtgctgtg acaaaaatct tgtcacagca cttttttgtt
    26521 gcgaataaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tctttaagct
    26581 gaccacttct gtcacaacct ctttagctta cgcttacgat tttttattgc ggtcttcaat
    26641 gtgtacatca ccgcttgtgt caatatccaa ttcttctcgg ctaacatctt ctgtcacttt
    26701 ttcaacatct tcatgttctt ctttatgaat cccaacttct tcacgaataa ctgtatgttt
    26761 gcttacatct acttgctctt ctttgattgg gatagtaatc gtgtcatctt ccaagttttc
    26821 gtctgttccc tgtgaagatt gatctgttac tggtttgcgt tcgatgacga tttcttcacg
    26881 tttgaccggt acttctaccg tttcagtatc gttgaccaca tgtttgtgga tgtcgacttc
    26941 tccagttgtc acatcatgtg tatttacatc taatttttct tcttttagtt gaattgtatc
    27001 atcatctaga tttggtttgc gatcgatgtc ttcagtcttg cttgtaccca ttgtatcagt
    27061 ctcgccaggt acgtccatcg gatcagttcc tggtgtcata ttcaatggat ccgttgtatt
    27121 ttgaaagcct gtcgtgccac ctacgtccat tggatctgtt ccagtgccag tttctaatcc
    27181 agtccccatt gtgcttgctg ggctaacagt atcttgtgta tccattgtat cttctgttgt
    27241 ttctggctga cgatagcctt ttactgtgat gacaaatttt ccatcagaaa tatctcctct
    27301 atattgatat aaaggatcat tttctggtgt agccgtctca gaatcatagg tatcaaaaga
    27361 aaaagtatct tttatttttt cccataaaga acggtcttct tcaccatcca tccgattttc
    27421 atctgtttca acatctatac cagacaatcc gtcaaatcct agtctagatg ctgcttcatc
    27481 agtagtataa ataacgatat catctcgttc atacccttcg ttacgcaacc gttcaattac
    27541 tgataatgct tcttccctcg tactatagct tccgatgacc gttaaatttt tctcttccat
    27601 gtaaattcct c
//
LOCUS       DAJSMI010000016        27359 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJSMI010000016 DAJSMI010000000
VERSION     DAJSMI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.016"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            158..1897
                     /gene="yfcC"
                     /locus_tag="ON430_000599"
     CDS             158..1897
                     /gene="yfcC"
                     /locus_tag="ON430_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR3218543.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2054..3238
                     /gene="iadA"
                     /locus_tag="ON430_000600"
     CDS             2054..3238
                     /gene="iadA"
                     /locus_tag="ON430_000600"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR3218544.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3349..3552
                     /locus_tag="ON430_000601"
     CDS             3349..3552
                     /locus_tag="ON430_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR3218545.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3653..4315)
                     /locus_tag="ON430_000602"
     CDS             complement(3653..4315)
                     /locus_tag="ON430_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218546.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4328..5830)
                     /locus_tag="ON430_000603"
     CDS             complement(4328..5830)
                     /locus_tag="ON430_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218547.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6378..8057
                     /locus_tag="ON430_000604"
     CDS             6378..8057
                     /locus_tag="ON430_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR3218548.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8184..8663
                     /locus_tag="ON430_000605"
     CDS             8184..8663
                     /locus_tag="ON430_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCR3218549.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8882..9562
                     /locus_tag="ON430_000606"
     CDS             8882..9562
                     /locus_tag="ON430_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR3218550.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9570..10901
                     /locus_tag="ON430_000607"
     CDS             9570..10901
                     /locus_tag="ON430_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3218551.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10916..11878
                     /locus_tag="ON430_000608"
     CDS             10916..11878
                     /locus_tag="ON430_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCR3218552.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11989..12327
                     /locus_tag="ON430_000609"
     CDS             11989..12327
                     /locus_tag="ON430_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCR3218553.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12406..13113
                     /locus_tag="ON430_000610"
     CDS             12406..13113
                     /locus_tag="ON430_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCR3218554.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13216..14697)
                     /locus_tag="ON430_000611"
     CDS             complement(13216..14697)
                     /locus_tag="ON430_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3218555.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15006..15542
                     /locus_tag="ON430_000612"
     CDS             15006..15542
                     /locus_tag="ON430_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3218556.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15620..16990
                     /locus_tag="ON430_000613"
     CDS             15620..16990
                     /locus_tag="ON430_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCR3218557.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17057..17392
                     /locus_tag="ON430_000614"
     CDS             17057..17392
                     /locus_tag="ON430_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218558.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17473..18330)
                     /locus_tag="ON430_000615"
     CDS             complement(17473..18330)
                     /locus_tag="ON430_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3218559.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18437..18952
                     /gene="tadA"
                     /locus_tag="ON430_000616"
     CDS             18437..18952
                     /gene="tadA"
                     /locus_tag="ON430_000616"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR3218560.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            18975..19059
                     /gene="ffs"
                     /locus_tag="ON430_000617"
     ncRNA           18975..19059
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ON430_000617"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19269..20654
                     /locus_tag="ON430_000618"
     CDS             19269..20654
                     /locus_tag="ON430_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3218561.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20691..21368
                     /locus_tag="ON430_000619"
     CDS             20691..21368
                     /locus_tag="ON430_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCR3218562.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21376..22140
                     /locus_tag="ON430_000620"
     CDS             21376..22140
                     /locus_tag="ON430_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCR3218563.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22142..22801
                     /locus_tag="ON430_000621"
     CDS             22142..22801
                     /locus_tag="ON430_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCR3218564.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22831..23340)
                     /locus_tag="ON430_000622"
     CDS             complement(22831..23340)
                     /locus_tag="ON430_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR3218565.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23452..24450)
                     /locus_tag="ON430_000623"
     CDS             complement(23452..24450)
                     /locus_tag="ON430_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3218566.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24651..25235
                     /locus_tag="ON430_000624"
     CDS             24651..25235
                     /locus_tag="ON430_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3218567.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25312..26505)
                     /locus_tag="ON430_000625"
     CDS             complement(25312..26505)
                     /locus_tag="ON430_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3218568.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            26569..26949
                     /locus_tag="ON430_000626"
     CDS             26569..26949
                     /locus_tag="ON430_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCR3218569.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27023..>27359
                     /locus_tag="ON430_000627"
     CDS             27023..>27359
                     /locus_tag="ON430_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218570.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTY"
BASE COUNT         8869 a         4643 c         5724 g         8123 t
ORIGIN      
        1 aaaaaaataa tcatacatag gagaaaaaaa acaaaataga aaaaagtgat tttctaataa
       61 attattcatg tggaaatgtc atttttactg tataattcat ttgattatcc attttttatt
      121 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
      181 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      241 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      301 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      361 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      421 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      481 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      541 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      601 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      661 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      721 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      781 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      841 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      901 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      961 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
     1021 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
     1081 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1141 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1201 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1261 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1321 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1381 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1441 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1501 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1561 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1621 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1681 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1741 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1801 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1861 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1921 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1981 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     2041 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     2101 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2161 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2221 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2281 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2341 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2401 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2461 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2521 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2581 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2641 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2701 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2761 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2821 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2881 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2941 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     3001 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     3061 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3121 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3181 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3241 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3301 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3361 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3421 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3481 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3541 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3601 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3661 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3721 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3781 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3841 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3901 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3961 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     4021 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     4081 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     4141 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4201 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4261 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4321 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     4381 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4441 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4501 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4561 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4621 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4681 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4741 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4801 tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4861 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4921 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4981 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     5041 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     5101 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     5161 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5221 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5281 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5341 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5401 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5461 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5521 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5581 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5641 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5701 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5761 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5821 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5881 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5941 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     6001 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     6061 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     6121 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6181 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6241 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6301 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6361 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6421 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6481 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6541 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6601 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6661 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6721 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6781 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6841 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6901 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6961 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     7021 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     7081 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     7141 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7201 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7261 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7321 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7381 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7441 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7501 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7561 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7621 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7681 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7741 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7801 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7861 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7921 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7981 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     8041 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     8101 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     8161 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8221 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8281 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8341 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8401 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8461 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8521 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8581 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8641 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8701 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8761 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8821 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8881 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8941 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     9001 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     9061 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     9121 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9181 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9241 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9301 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9361 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9421 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9481 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9541 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9601 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9661 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9721 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9781 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9841 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9901 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9961 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
    10021 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
    10081 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
    10141 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10201 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10261 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10321 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10381 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10441 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10501 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10561 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10621 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10681 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10741 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10801 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10861 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10921 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10981 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    11041 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    11101 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    11161 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11221 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11281 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11341 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11401 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11461 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11521 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11581 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11641 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11701 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11761 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11821 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11881 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11941 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    12001 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    12061 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    12121 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12181 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12241 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12301 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12361 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12421 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12481 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12541 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12601 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12661 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12721 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12781 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12841 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12901 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12961 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    13021 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    13081 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    13141 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ataagaggat
    13201 gtgacaagaa ttttgtcaca tcctctttgt tttgttctca taaccttttt tcatatacag
    13261 ttcatgaacc gcttttatta acttgccaaa taacactgta ttgtgcgttg tatccagata
    13321 gaaagtctcc gtttcatctg aaattttctc atgctgatct gtaatgatca aatcagcatg
    13381 ttctgaatat gcggtaattt taacagcatc tttgcgaaat aactgctgca aatggctttg
    13441 aagatgataa tttcctgtaa actgtttggt tatctgcaaa tggatgagta actgctcttt
    13501 cgtttctgtt tgtagtaagt gttggatcaa ttgactaagg tattgactga tttggtttct
    13561 ttccccattt tttgaaaaaa ttgtatcgat tttttctctt aatttctttt cctgtataaa
    13621 agaactagaa tgattggacg tggcttctgg aaagcaaagt gtagaaaaac tactgctgga
    13681 ttcacccaac agttcgaaaa aaacaactgc caacacaaga aaataaaaat aaattggtat
    13741 tttattttct aaatggttga gaggaaactg ttgagttaag taagtcatca attgaccgac
    13801 ttgtcgacaa ataaggtttt tgcttgtgga aaataaaaaa ccaatctcct gtttttgtcg
    13861 aaaagagatt tgatttggaa aaaaacaata gaaaaaataa ttaaagtagg ctctctctgt
    13921 ttctgtctct tcacaagggc gactcactga aaaagaagta aagtattctg aataatcatg
    13981 tgtatgctgc aataagtcca tcaattcagt gatttcagaa gaagtttttg gaaaatatgc
    14041 ctgttgtttg tttcgtaata tgaataaagt taacagcaag tagatatttt tttcttgttt
    14101 tgaaattttt tctatttgct catctgtact gttcattttt agtgcattta attcaagtga
    14161 ggaaaatggc caatcgttac ctccaaatgt atctgaaaga aaaaagtaaa gatagagacg
    14221 gatagataac tcatttcctt caagagtaca gcaatttttt acagatagga tccgtaaatg
    14281 gaattttttt aaggaatgat tgagctgttt tgtcagtttt tcgagatagg agggcgatat
    14341 ataaagttgt tcaagtaaat atttttttgg tacagcagtt tgtggataat tgattagtaa
    14401 catcaaatag ttaaaacgat tggattcttt taggtagcat aattttaact tagaaaaaac
    14461 agctaacttt ttagtttgat cggtttcttt ggtttggatc tctcctttat gattagaata
    14521 aataaattca tcaatcaata aaaattgctt gagtgataca tttatttttc ggatcagttc
    14581 tttgattgtc cgttttggaa tatgtaagtc attggataat tcttgtaaag aacaattatt
    14641 tttttgaatg accatttgta gaacaagtaa ctcttttttt cttgcttcaa ttagcataaa
    14701 tacctccttt ttattctgtt tttgtatata gttagacaaa aactgtgagt ggaaagtcat
    14761 ttcagtctgt gttattctca cagagtgtcg ccttcttttt tatataacgt tatttaattt
    14821 ttttgttacc ttgtttattc tattgtttat ttcctagggt attttgtcaa gatattcttg
    14881 tttttaaggc ggaaaaattg atttttaatg gatataacta aatatgtttt gataattatc
    14941 aataatattt ttagaattat ttatgttatt tatttatggg tagatgaaaa gagacggagg
    15001 taagaatgat ggggagagta acaaattata gtaaagaata tttgctgttc aaaagtatgg
    15061 tatacgtgga agaatatgga atgaagtcaa ttactgcaag agagttagca gagttctgcg
    15121 gttgttctac atacccgatt tatacacatt tcaaatcaat cagcgggtta aaagagaaga
    15181 tattagaaga aatcactgtt tattttgaaa actacttagc tgaatgcagt tccgatgatg
    15241 ttttaagcat tgtctactta ttgaaagatt ttttttctat ccatgaaagt attcgtgaaa
    15301 tcattgcaaa atctgattta gacatggatt cgctatttaa gcggccattt ttctcatatg
    15361 tcaaaggtca cacgaagatc aaagaagaaa agtcgcttga attggtatgg cttaatgcat
    15421 tgggaagttt gcgctcagat gattcttctc aagattttct tgaaatgatg aacgctgttt
    15481 gggataaaaa cgaggtgacg gatgaatact tggaaaatat cttaaaggaa cagtcacctt
    15541 aggcagcaac atgaaaaagt agatcagcca cctttatttt tcgtaaaaaa gattgtaaaa
    15601 tgtctaacga aacattggaa tgaaggagga agcgacaacg ttaccacagc aatttatcaa
    15661 aaaatatcgt cttcttttag gcgaagaagc atccgatttt ttttctgcgc ttgaacaagg
    15721 aagcgtgaaa aaaggatttc ggtggaatcc actaaaaccg gcaggattgg acatggtcca
    15781 aacttaccat agcgaagaat tacaacccgc tccctacagc aatgagggat ttcttggcac
    15841 tgtcaatgga aagtctttcc tccatcaagc tgggtatgaa tatagccaag aaccaagtgc
    15901 aatgatcgtt ggaacagctg ctgcagcaaa acccggtgaa aaagttttgg atttgtgtgc
    15961 ggctcccggt ggtaaatcaa cccaattagc tgctcaaatg aaggggaaag ggttattagt
    16021 gacgaatgag atctttccga aacgtgcgaa gattttatca gaaaatatag aacgatgggg
    16081 agtttcgaac gcaatagtga caaatcatgc acccgcagaa cttgtccctc atttttcggg
    16141 cttttttgat cggatcgtgg ttgatgctcc ttgttcagga gaaggaatgt tccgaaaaga
    16201 tccaaatgca ataaaagagt ggacagaaga atctccgcta tactgtcaaa aacgtcagca
    16261 agagattttg tcttcagcaa taaaaatgct aaaaaataaa ggacaactga tttattcgac
    16321 ttgtacgttt gcaccagaag aaaatgaaga gatcattagc tggcttgtag aaaattatcc
    16381 agtaacgatt gaggagattc ctttgactca atccgttagt tcaggacgtt ctgaatgggg
    16441 aagcgtagct ggtttggaaa aaacaatacg tatatggcct cataaagatc agggtgaagg
    16501 acattttgtt gcaaagctga cttttcatgg acaaaatcag atgcataaag aaaagaagac
    16561 acgaaaaaaa agtaaagtgc aaatgacaaa agagcaggaa aaactctgga cggagttttc
    16621 aaatgatttt cattatgaag ctactggaag acttttagtg ttcaacgacc atctttggga
    16681 agttcctgag ttagcccctt ctctcgatgg tttaaaagtc gtacgtacag gtttgcattt
    16741 aggagatttc aaaaagaatc gatttgaacc tagctatgca ttagcgcttg ctacaaaaaa
    16801 gatagagaat ataccttgct tgccgatcac tcaaaaggaa tggcaatctt acacagcagg
    16861 agaaacattt caacgtgatg ggaatcaagg atgggttttg ctagtattag ataagatacc
    16921 tgtcggattt gggaaacaag taaaaggaac agtaaagaac tttttcccta aaggattgag
    16981 atttcactga gtggattagt tcgtttattt ttttgtctat gctatgatgg agatagataa
    17041 aaaggagctg atacttatga aaacatcaac aaaagtaaca atcggattaa gcgtagcagc
    17101 cgctgccagt gtagcgacag cagtcgttgt atcgggaaaa gtaatggaaa agatccatca
    17161 tatgactact cggacgaaag taaaaaaatt cgtctatgac aaatttgatg gaaatgaaaa
    17221 attactagaa atcgtagatg gtctttcaga tagtgacttg gattctttga tgggaatgtt
    17281 agcaaaaatc aaatcaggaa aaaagaaaat ctctgtttat ggtgattcaa tcaaagattc
    17341 aaccgaagat gtaaaagacc ggctaatgca ttttgttgaa aaaatgatgt aagagaaact
    17401 agcttttgaa cactattcat tcgaaataag gggttgtgac aaaattattg tcacaacccc
    17461 tttaatctat ctttaattat gttcatgaag caataaaaga tagcaaaaca gtaaatcgtt
    17521 ggcttttttt agattgaaac cagactgttc ttgaaatttt tcgattcgat attttagagt
    17581 attacgatga agaaacagtg tttttgcagc agagctgaca ttgcctaatt cattccataa
    17641 ttcttgaatg atcacttcca tttcattttg tttgatcagt tctatgtaac ttttgatcaa
    17701 cttactatct tttacagtat cttttgtata gtaatgtaaa gccacatctt gcatagaaaa
    17761 agtacggctg tgagtataaa gattgttttg ctctgttaaa aaaatttttc tttcttcagc
    17821 aaacaaaggg acgaggtcgg tgccaggtga atggaaagaa ccaacgaata cagacgttgt
    17881 cgtatcaaaa tcagcatcta aagatagaaa aataccgttg atttcagtag catcaagata
    17941 atcacggttt atttgttcga ctaagagcgc atctgtgtct gtataaaaaa agaaatcagc
    18001 agaatagcga aataattcca aaatagtatc ttgccattct ttttttaaga attctccttt
    18061 tgattcgacg tgaagctgga tcaaacgaaa gcttttttct tgaacacaag aagcatcttt
    18121 aaaaagatag tgataccatg ggtgtttttt ttgatcgttg attacaggga ataacgcttt
    18181 caaaaggctg gtttcttgtt gagataatga atcttgtttg atccagataa aagcatcatc
    18241 aattgcaaaa gaaaggatat ccgcttctga tgctggagag ttttttttct gtgcttgtgg
    18301 gtaaagagca agtaatttat ccattttcat cttgttcacc tcttgtattt attttaccat
    18361 agaaaaaaaa gttagttaca gcctttttat catatgctaa agtaaaacaa gaaaaaacaa
    18421 aggcagggaa gggtaattgg taaatacaaa tcttgatgca gtagaaaaag aaaaatggat
    18481 gcgccttgca ttggcagaag caaagaaagc agaaatgctt catgaagttc cgattggagc
    18541 agtggttgta ttggatggaa aagtgattgg aagaggatat aaccttagag aaaccacgca
    18601 agatgctact acacatgcag aaatgcttgc aatcaaggaa gcctgtgaaa aagtgggaag
    18661 ctggcgcttg gaagatgctg cactttttgt taccttagag ccttgtccta tgtgcagcgg
    18721 cgcgatgatt ttgtctcgtg tagcagaagt ttatttcggc gcgtatgatc caaaaggcgg
    18781 aacagccggt accttgatga atcttttgga ggacgagcgg tttaaccacc aagcatatgt
    18841 agaaggtggc attttagaag acgaatgtgg gatgattttg actgactttt tccgaaagct
    18901 ccgccagcga aaaaagaaat taaaaaaaga ctggtcaacg aatgaaaaat aaggtatact
    18961 attctttgcc gaaaggctag gacaatggtg ggcttgtgaa gcgtgtcagg tctggaagga
    19021 agcagcacta agcaggttcc gccatgtgtc tgattataat gaaacgacaa cagactttcg
    19081 ggtctgtttt ttttattttt atagtactgt aaaaatgaat gttcctgatt ttttagccag
    19141 actgatttat cgaatattgt acttatacaa tttgcttcaa ggcaggaaaa actctgaaat
    19201 agagcttgtt tcaatggaag aaaaagctgc ctatggtatg ataaagataa agagaggcga
    19261 gagggtacat ggaaaaaata gtgattttac atacgaatga tctgcattct catttagaaa
    19321 attggccaag gatcagaagg tttttggatc aacgtaaacg agaaaacgaa aagaaagaaa
    19381 aaacgacaac gattgcagta gatctaggtg attttgtgga tcgttggcat ccattgtcag
    19441 aagcaacgaa tggacaggaa aatatcttgc tgatgaatga agttggttat gatgcagcga
    19501 cgataggaaa caatgaaggc gtaggaaatt ccaaagaaga gttgaatcat ttatacgatc
    19561 atgctgagtt tgatatcatc ctggataact tattcgataa aaatacgctc cagccaccgg
    19621 catggacgaa accatataaa atcattacaa cgaaacacca gacgaaaatc ggactaattg
    19681 catttactgc tccatttccg cttacttata atccgaatgg ctgggacata cggaatcctt
    19741 atgatatttt gccagaatta gtcgaagatt tacgttcaca agtggatgtt ttagttttga
    19801 tgagtcatct cgggattcag gatgatttcc aaattgcaaa agaaatacca gcaattgatg
    19861 ttattttagg atcgcacacc catcatttat tcagagaagg tcacgtaatc aatgatgtcc
    19921 aaatagcagc cgctggaaag tacgggcagt atgtcggtga agtccattta accatcgatg
    19981 aagagaaaaa aatccttaag cattcagcca aagccatccc taccgaaaca atgacagctt
    20041 ttacagaaga tgaacaagaa ataaatagtt atctagaacg tgggcatgaa ttattaaaag
    20101 aaaaagaagt agcagatttg ccattttctc tgagtctaga tatcttcaat gagcattcgt
    20161 ttatccaagt agcctttgag gcggtgaaag aacgaggaaa gacggaagca gcaatattaa
    20221 acagtggtct gtttttaact gaaattccaa aagggcgggt caatcaggat cagcttcaca
    20281 ctgcgttgcc gcatccgatg cacttgttaa atgtcacatt gggtggaaat gatctgatcc
    20341 gcttagtttt agaaatagaa aaaaatcgta actttttacg taactatcct atgaagggaa
    20401 tgggatttcg cggtaagatt tttgggcaga tggtctataa tggaatcaca tatgattcta
    20461 ttaaccatca agtcttttgg aagaacaagc caatcgatct gaataaaaat tatactttta
    20521 ctaccgtaga tcatttgatg tttgttccat tttttccaac gattgagatt gctggaaaaa
    20581 atgaattttt gttccctgaa tttatccgaa gtgtggtagg agattacttg aaagctcact
    20641 acccaatcaa ataaaaacaa ggtataataa aaaagatagg tggtgaacgc atgtctgcaa
    20701 aacaaaatga acagcagaac gttgagcata aaacagaaat tgctaagaaa aaatcatcag
    20761 ctgaacagga aacaacagtc accgaagaac tgacagctgc tttggaagaa atcgttgagg
    20821 agcctaaagc agaaaaagtc aaaggcgagc tagtgacact gttagatgac agtaattttt
    20881 tgataggtga aagacattat cggcttgtgt ccgattatcg tgaaggattt aatgcagaaa
    20941 agcttggaga acgttacagt gatgtactag cccgatatga ttatatcgta ggagattggg
    21001 gttatgaaca actacgccta aaaggatttt tccaagcgga taatcgacgg gcgcatccag
    21061 atcagcgaat cgattccttg gaagactatc tttacgaata ttgcaatttt ggttgtgcgt
    21121 attttgtcat cgagagaatc ggcggtaaaa aagaaaagac aactcagcgc cgaaaaaaga
    21181 aaaaaaacca taatcgtgcg tatatcgatg agaaaaaagg accagtcgca tctaatcgca
    21241 aaaaaccggt tattaaaaat cgaaaaacag agtcagcaaa aacctctgaa aaagtaaaaa
    21301 aacaagaaca acctaaacaa gtagaaaaaa caaaaacagc atttacgatc agaaaaaggg
    21361 aagagtagtg gatgaatgga ctataaagga tatttaattg atttagacgg aacgatttat
    21421 cgtgggacac aacccatacc ggccggtaaa cgatttgtgg aagaattgca aaaaagaaaa
    21481 ttacctttct tgtttgttac caataacacg actaagtcac cagaaacagt agctaatcgt
    21541 ctagcagatg agtttgacat tcatgtagct ccagagacag tctacacagc tactttggca
    21601 acaatcgatt ttatgaaagc agatggaaaa gggaaaaaag tctatgttat cggagaagca
    21661 ggattgattg acttgatttt agctgccgga tttacatggg aagaagaggc tcctgactat
    21721 gtagtggtag gcttagataa ttatctgacc tatgaaaaag tagttaaagc aacacttgct
    21781 attcaaaaag gtgctacttt cattggaacg aacccagata agaatatacc tactgaacgc
    21841 ggccttcttc ctggagcagg gtcagtcatc tcttttgtag agacagcaac ccaaacgccg
    21901 ccgatctaca tcggaaaacc agaagccatc attatggata aagccgttga agtattaggg
    21961 ctgcaaaaag aagaagtaat catggttgga gataactatg aaacagatat ccaagctggg
    22021 atacgcaata atatagatac tttgctcgtt ttatcaggtt ttacaaaaga agaagatgta
    22081 cctgggttgc ctatcccagc tacctatgtg aaacaatcac tggatgaatg gagcttctaa
    22141 tatgaaaaat tcgagatggc agtggatgga atatgctgga ttgttcagcc tatttctgac
    22201 actgatcagt ctggcagttg gtgtgacgat caactttcga ccactatacg tatttgatat
    22261 cggacatttg caaattttgg attatacttc tttagatcaa gagacgttac tgaaaaattt
    22321 tgatcaccta atgaattatc tcaataatcc cttcaaaacc atcctcagct tgccagattt
    22381 cccagtttct gctagcggag cccatcactt ttatgaagtg aaaattttgt ttttagtgga
    22441 ttatgctgtg tttttcatca ctttgatccc gagtattttg tttatcaaat atttgcaaaa
    22501 gaatgatcgg ctttggcggt tgattcgtcc gtttcaaatc ggcatgttac tgccagttgt
    22561 tttcggtttt ttcatgatga taggatttga tcgctttttt atcctttttc atgaaacgtt
    22621 tttcaacaac gatgactggt tattcgatcc cgttactgat ccaatcatca atgtacttcc
    22681 agaagaattt ttcatgcata gctttatctt attttttgta ttattagaat tattttttgc
    22741 tgtttttctt ttcttaggaa aaaatagctt gaaacaaaca aaaaagaaag aactcgtcta
    22801 aatagacgtt ttctttcttt ttttgttgaa ttattgagta aggcgacttc ttttttctgg
    22861 ttctttgtgt gctaatcgac tggcagcagc agcggcgatc gctccgacga tatcatctaa
    22921 gaaggtatgg acatgaacgc catcgtggct gtttagttct tttaaaatac caggtttcac
    22981 cttgtcgata taaccataat tcgtaaagcc gatcgtaccg taaacattca cgattgacaa
    23041 ggctaggatc tcatcgatac cgtaaaggcc ttcgtcatct tcgataatat gttgcaacgg
    23101 actcatcaat tttttctttt ctgctaatat atctaattgg attccagtga taatggcgtt
    23161 atgtacttca cgctttgtta gcacgctgtt gacactttca gtacaatccg ccaaagtcaa
    23221 atcagaaata tagtcttttt gtaaaaacat gacaagttgt gcgatatctt ctacagttac
    23281 gccacgttcc ttcaatagtt ctcttgcctt ttcttctaaa gtctcagttt tgacgaccat
    23341 attattgctc cccctttatt ttttctttct ctatttcatg ttaacggaac atgaaaaaaa
    23401 tgcaagaaac tgctttcggg tttaacttga aatgcaaaga gatataaaaa atcaactagg
    23461 acgaagattt ttatcgtata agcttgtgct gtgaccaggc tgagttcgtt cctttggatt
    23521 aatatagtgt aaagcgttgt tcactgcagt aggtgcttca ccgagcccag tagcaatcag
    23581 tttcactttt ccttcatagg tacagatgtc accagctgca taaacgcctg gaatagaagt
    23641 agacatatct gattttacag taatggcatt tctggtacta tcaagtcccc aagaagaaag
    23701 atgttctaaa ttagaagaga atccataatt gacgatcaga gaatcaatca tcaaatcaat
    23761 cgtctcgtca cttttcactt tttttaaccg gatatctgtc agttccccat tattgcctga
    23821 taatccatcg atgagatagg gagtaagcaa gttgacagat gaagatttca agcgtgatac
    23881 actatgttcg tgcgcacgaa attccgggcg acgatgaatc aagtaaactt cgcttgcaat
    23941 aggctctaac atcaacgtcc aatcgatagc agaatcacca cctccagcaa tagcgacttt
    24001 ttttccagca tacttcatca aatcattcac gaaataatcc aatccgtggt tttcaaatga
    24061 ttcagcatta tctaaattca attttcttgg ctggaatgaa ccatttccta aagctaaaat
    24121 gactgctttg gaataatgga tgcctttatt tgtagtaatc tcgataactt cgtcttcacg
    24181 agtaagcgag agaacttctt cttttaaatg gaatgtatgg ttaaaagtag ttaattgttt
    24241 ttctaattga tcgattaatt cacttgcttt tattgctgga tacccaggaa tatcgtaaat
    24301 atatttttcg ggatacaaag ttgctaattg accaccaagt tgaggtagac tgtcaatgat
    24361 ttttgtttta gcttggcgca ttcctgcgta aaaagcggca aacatcccta caggacctgc
    24421 accgatgatt gtaatgtcat agatttccat aaaaattcct cccttttcac actatagcag
    24481 aatttaattg agaatgcttc tcaaaaatgt ttgaattcga aatcgaaacg gtaagttata
    24541 gaatgtatct agtgatgaaa aataaaagaa aacaaatcta gtatttttac gagaggttag
    24601 gtattatgtt attattatta tgaagtatgc aaatattagg aggaaacaat atggcattcc
    24661 cacaactaga tttaaaaagt gaaaaaggac caaaagctgt tatcaaaaca aaccgtggtg
    24721 acattactgt tcaattattc cctgaactag caccgaagac ggttcaaaat ttcattgaac
    24781 tttccaaaaa aggttattat gatggcgtga tttttcaccg cgttattcct gatttcatga
    24841 tccaaggcgg cgatccaact ggaacaggaa tgggtggcga aagtatttat ggcgaaagtt
    24901 ttgaagatga attcagtcgt gaactgttca accttcgcgg agctttatct atggcaaatt
    24961 caggtccaaa cacaaacgga agccaattct ttattgtaaa taacacaaat gtgccagcta
    25021 atatgcttgg acaactagaa ggtgctggat tcccatcaga aatcattgaa gcgtataaag
    25081 gtggcggaac tccttggcta gatttccgac atacagtctt tggacatgta ctagaaggga
    25141 tggacacagt agatgaaatc gcaaatgtac aacgtggtcc acaagaccga cctgtacatg
    25201 atgtagtgat tgaaacaatt gaaatttcag aatagtttgg tatggtacgg agtaattatg
    25261 aaaaaggacg aaacctagta aggtctcgtc cttttatttt gaataaatga tttaatcgat
    25321 tgtctctaaa gcatacttga ttcgattaag tccttccagt aaagtttctt ctggacaagc
    25381 aatattcaag cgcatatgcc catgaccgga aggaccaaac gtgatgccag gatttaatac
    25441 gacttttcct ttccgaacaa gagtgttctc caattcttgg tcgtctgaaa tataggcggt
    25501 aaaatctaac cacattaagt aagttccttc agggttcatt attctcactt tcggaagata
    25561 ctgttcaaaa aactgattaa ctgtttgaac atttttttct agataaggaa tcagttggct
    25621 taaccattct ccacccgtct cataagccgc ttgggttcca atcagtccaa aagtgttgat
    25681 ttcgtgctgt tggttcatca ctaaatgttt ttcaaaatca gctttcatct ctggattttt
    25741 gataaatacc attgaatttt tgattgcagc tagattgaaa gtcttggttg cggaagtaaa
    25801 agcaactagt agatcatcta aattaggatc aatcgttagc atggaagtaa atgtatgatc
    25861 gaataaagtt aaatcttgat ggatctcgtc actaaacaga atcacttgat gttttaaaca
    25921 caattcgctt aactgtttca attcttcttt gctccaaaca cgccctccag gattgtgagg
    25981 attgcataaa atcattgctt ttacattgtt ttcttcgatt tttttctcca tgtcagacaa
    26041 gtccatgatg aaatgattat ttttttctag caaagagctt cgaacgatgt gacgttgatt
    26101 tgtttctata atagaggaaa atggaggata aactgggtca tgaatcaaaa tagaatcgcc
    26161 tggttttgta aaagtttgta ctgctgcagc taaactggtt aacacaccac ttgtaaaaac
    26221 gatattttct ttcgtcaact ccacagcatg gtgttggcgt tcccaatgaa tgatagcctg
    26281 atatagttta tcagacagag tggcataacc aaagattcca tgtttgatgt attcactaaa
    26341 cgctttagct accccaccgg gagacaagaa gtccatatct gctacccaca aaggaagtaa
    26401 gtcatccatt tgatatgtct cagcaattgc gtcccatttt acactttctg tatcttttct
    26461 tgaaatctgt ttatcaaatt cgatcatctt aagacctcca gtcattttcg tcgcttcttt
    26521 ttttgagcta tttacagtat aatgataaat gcgaagagag ggaagtagat gacttataaa
    26581 attggacaaa taattgaagg aaaagtgaca gggatacagc cttatggcgc attcgtttct
    26641 cttgataacg agacgcaagg acttattcat gtttcagaag tacaatccgg ttatacgaaa
    26701 aatatccatt cattgttaaa agtcggacag ccagtaacag ttcagatcat cgacattgat
    26761 gaatattcca aaaaaatcag tttatcttta agaacgttag aaaaaacaaa tccagctgtt
    26821 ccatatcgca gaaaaaggta tttcacgaat aaaaatcgta agatcggatt taccactatt
    26881 gcggagcagt tacctatttg gatagaagaa gcattatctg aactagaacg gaaagaaaat
    26941 aagaaataaa aagtgtattt tttctgaaaa tgtgctagaa tttttatgta agccatagaa
    27001 caagttgatc gaggtggaaa ttttggcaag caaaagagtt gctggtacaa ttatgttgca
    27061 tttggaggat ggtactaaaa aatttttggt ccattcaatt gatgacaagt tggaatttgc
    27121 gttagcggaa ctttctgaag gaaagactgg tttagcgaat atacttaatt tcttaaaaga
    27181 aatagttcat atcgatgtta gcaagattag tttgatggaa ttgacaaata cacatattaa
    27241 agaggaaaat gttccgttgt ttgtctttga aactgaacaa aagaatcaaa gagaagaatt
    27301 aggcgaagga tatatttggg aagaaccagg tcaaatgcgc tctgttcttg gaacttacg
//
LOCUS       DAJSMI010000017        27218 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJSMI010000017 DAJSMI010000000
VERSION     DAJSMI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.017"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(16..384)
                     /gene="rplL"
                     /locus_tag="ON430_000628"
     CDS             complement(16..384)
                     /gene="rplL"
                     /locus_tag="ON430_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR3218571.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(437..940)
                     /locus_tag="ON430_000629"
     CDS             complement(437..940)
                     /locus_tag="ON430_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR3218572.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(1144..1271)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1350..2039)
                     /gene="rplA"
                     /locus_tag="ON430_000630"
     CDS             complement(1350..2039)
                     /gene="rplA"
                     /locus_tag="ON430_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR3218573.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(2143..2565)
                     /gene="rplK"
                     /locus_tag="ON430_000631"
     CDS             complement(2143..2565)
                     /gene="rplK"
                     /locus_tag="ON430_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR3218574.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            2908..3576
                     /gene="sdaAB"
                     /locus_tag="ON430_000632"
     CDS             2908..3576
                     /gene="sdaAB"
                     /locus_tag="ON430_000632"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCR3218575.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            3589..4461
                     /gene="sdaAA"
                     /locus_tag="ON430_000633"
     CDS             3589..4461
                     /gene="sdaAA"
                     /locus_tag="ON430_000633"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCR3218576.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(4552..5571)
                     /locus_tag="ON430_000634"
     CDS             complement(4552..5571)
                     /locus_tag="ON430_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR3218577.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(5744..6649)
                     /locus_tag="ON430_000635"
     CDS             complement(5744..6649)
                     /locus_tag="ON430_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3218578.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(6708..7253)
                     /gene="nusG"
                     /locus_tag="ON430_000636"
     CDS             complement(6708..7253)
                     /gene="nusG"
                     /locus_tag="ON430_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR3218579.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(7354..7524)
                     /gene="secE"
                     /locus_tag="ON430_000637"
     CDS             complement(7354..7524)
                     /gene="secE"
                     /locus_tag="ON430_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR3218580.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(7548..7700)
                     /gene="rpmG"
                     /locus_tag="ON430_000638"
     CDS             complement(7548..7700)
                     /gene="rpmG"
                     /locus_tag="ON430_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3218581.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            7862..8506
                     /locus_tag="ON430_000639"
     CDS             7862..8506
                     /locus_tag="ON430_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3218582.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(8562..9065)
                     /locus_tag="ON430_000640"
     CDS             complement(8562..9065)
                     /locus_tag="ON430_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR3218583.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(9078..9124)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            9662..10627
                     /locus_tag="ON430_000641"
     CDS             9662..10627
                     /locus_tag="ON430_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR3218584.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(10726..11898)
                     /locus_tag="ON430_000642"
     CDS             complement(10726..11898)
                     /locus_tag="ON430_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218585.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(11885..13030)
                     /locus_tag="ON430_000643"
     CDS             complement(11885..13030)
                     /locus_tag="ON430_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218586.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(13044..13976)
                     /locus_tag="ON430_000644"
     CDS             complement(13044..13976)
                     /locus_tag="ON430_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218587.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            14093..15172
                     /locus_tag="ON430_000645"
     CDS             14093..15172
                     /locus_tag="ON430_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR3218588.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            15169..15798
                     /locus_tag="ON430_000646"
     CDS             15169..15798
                     /locus_tag="ON430_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3218589.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(15908..16135)
                     /locus_tag="ON430_000647"
     CDS             complement(15908..16135)
                     /locus_tag="ON430_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3218590.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(16238..18826)
                     /locus_tag="ON430_000648"
     CDS             complement(16238..18826)
                     /locus_tag="ON430_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCR3218591.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(18846..19505)
                     /locus_tag="ON430_000649"
     CDS             complement(18846..19505)
                     /locus_tag="ON430_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3218592.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(19554..20057)
                     /gene="trmL"
                     /locus_tag="ON430_000650"
     CDS             complement(19554..20057)
                     /gene="trmL"
                     /locus_tag="ON430_000650"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR3218593.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(20278..21144)
                     /locus_tag="ON430_000651"
     CDS             complement(20278..21144)
                     /locus_tag="ON430_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3218594.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            21253..21894
                     /locus_tag="ON430_000652"
     CDS             21253..21894
                     /locus_tag="ON430_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR3218595.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(21956..23317)
                     /gene="mgtE"
                     /locus_tag="ON430_000653"
     CDS             complement(21956..23317)
                     /gene="mgtE"
                     /locus_tag="ON430_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCR3218596.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(23335..24234)
                     /locus_tag="ON430_000654"
     CDS             complement(23335..24234)
                     /locus_tag="ON430_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3218597.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(24236..25033)
                     /locus_tag="ON430_000655"
     CDS             complement(24236..25033)
                     /locus_tag="ON430_000655"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCR3218598.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(25011..25715)
                     /locus_tag="ON430_000656"
     CDS             complement(25011..25715)
                     /locus_tag="ON430_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCR3218599.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            25846..26421
                     /locus_tag="ON430_000657"
     CDS             25846..26421
                     /locus_tag="ON430_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCR3218600.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            26539..27216
                     /locus_tag="ON430_000658"
     CDS             26539..27216
                     /locus_tag="ON430_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR3218601.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         7926 a         5744 c         4904 g         8644 t
ORIGIN      
        1 tttaaatagt aaaaattatt taacagttac agatgcgcca acttcttcaa gtttagcttt
       61 caattcttca gcttcttctt tagatacgcc ttctttaact ggtgcaggag cgccatcaac
      121 tacagcttta gcttctttca agcctaaacc agttgcttca cgaactgctt tgataacttt
      181 aactttttgg tctccagctg cagttaattc tacagtgaat tctgtttgtt cttcagcagc
      241 agcagcgcca ccagcagcag ctacagctac aggagcagca gcagatacgc caaattcttc
      301 ttcaatagct ttaactaggt cgttaagttc taggatagtt gcttctttta actcagcaac
      361 gatgttttca atgttcaatg ccattataat ttcctccgtt taattgttct tttttattta
      421 atgctttatg cggcaattaa gctgcatctc catcttcttt gtctgccact gctttgacag
      481 cgtaagccac gttgcggact ggcgcttgta gtacagatag caacatagat agaagtcctt
      541 cgcgatttgg caattttgcc aatgctgtga tttcttccaa tgaagagact ttaccttcga
      601 tgataccgcc tttgatttct aatgcttttg cttctttagc aaattcgtcc atgattttcg
      661 ctggtgcgac tacatcttcg ttactgaatg caacagctgt aggtccagtg aatacttcgt
      721 ccatgccttc aaggccagct tttttagctg cacgtgaaag aatagaattt ttgataactt
      781 tcatttctac gttagcatca cgaagttgtt tacgtaaacg tgttacttca tcaactgtta
      841 atccgcggta gtcaacgact actactgatg cagctgctgt aaatttttcg cttacttcat
      901 caacgattgc tgcttttttt gcgataattg cttcactcat ttttgagttt cacctcctga
      961 attttgatgg aagagttttt tgcactgaaa aggcaagtca cttttatagt tactttgctc
     1021 aaccagtatt attcatcacc tattcacttc gttcatagtg attctactcg ctttgctcga
     1081 aaggaaactc ctttcgcttt gctcaaccag tattgttcat catctcttca cttcgttcat
     1141 agtgattcac aataaaaaac tctatgccac cgcagacata gagggactat tgaatatatt
     1201 caataaacgt cctcggtagg aaattaaggc ttgatgccac ctactgtctt cggtacaagt
     1261 taattattaa cctcaactag cttagcatgc taaactagtt gagtcaagaa ttatttttca
     1321 gttggatcaa tcaacattct aatatttaat tagaaagaag cttggtcaac atggatacca
     1381 ggtccaaatg ttgttgtgat cgttacattt ttcatgtatt gacctttagc tgctgatggt
     1441 ttagctttta gcaatacatc atggattgct gcaaagtttt caactaattt ttcatcgtca
     1501 aatgaaactt ttccgattgg tacatggata tttccagctt tgtccacacg gtaagttact
     1561 ttaccagctt tcacttcgtt tactgctttt gttacgtcca ttgtaactgt accagtttta
     1621 gggtttggca ttaagccttt tggtcctaat actcgtccta gacgacctac agtagccatc
     1681 atatcaggtg ttgcaacaac tacatcaaag tcaaaccatc cgttttggat tttttgaacc
     1741 atgtcgtcgt cacctacgaa atctgcacca gctgcttcag cttcttttgc tttgtctcct
     1801 ttagcaaaaa ctaaaacagt ttgtgtttta ccagttccgt ttggtaatac aacagcacca
     1861 cggatttgtt ggtctgcttt tttagggtct acgttcaaac ggtatgcaac ttcaactgtt
     1921 gcatcaaatt ttgcaatgtt tgtttctttt gctaaagcta ctgcttcggc aacaggataa
     1981 actttacttg aatcaacttt ttttaatgca tcttgcattt ttttgctctt tttagccatt
     2041 ttggttcctc cttgattgtg gttataacgg ttgtacctcc cacgtgttct atatctttca
     2101 atatagagcc aactgagaag ctgctgtctt agggtgtata gattattcta cagtgatccc
     2161 catgcttcgt gcagttcctt caaccatgcg cattgctgct tcaacgttag ctgcgtttag
     2221 gtcttccatt tttaattcag cgatttctct tacttgatcg ctagaaactt tcgcaacttt
     2281 atttttgttt ggttcgcctg aacctttttc gattttagct gcttttttca atagaacagc
     2341 agctggcggt gtttttgtaa cgaatgtgaa tgaacggtct tcgtatacag aaataacaac
     2401 cggaatgatc aaacctgctt gatcagctgt acgagcgttg aattcttttg tgaatcccat
     2461 gatattgata cccgcttgac ctagtgcagg acctactggt ggagctggag ttgctttacc
     2521 tgcaggaatt tgcaatttaa cgattttttc tactttcttt gccacgagac atacctcctt
     2581 aagtccgtga tgtggttaat tggagatgca tatttctcct cccacgtttt atctgatctg
     2641 aaaaaatcca gacaaataat gtgcaaaaca atgcacactg gtacagtata gcaaatttat
     2701 ttgttttttc aagatttttt tctgctattt ttacattttg cttgaataaa cgctaaattt
     2761 attctaaaaa aacaacaatt tttcgttctc atttcttctt ttatgttaca cttgcgctag
     2821 catggttcaa cagatgaaag caaacaataa cacggttttt tagacccttg tacaaagatt
     2881 cctacataac aaaatgaggt ggagaaaatg aaagatttaa ggtatagaag tgtctttgat
     2941 attattggac ctgtgatgat tggtccaagt agctctcaca ctgccggagc tgctagaatc
     3001 gggaaaatca tacgtagtat atttggcgaa cagcctgatt ctgtagatat ttatctatat
     3061 gaatcatttg caaaaaccta tcgggggcac ggaacagata ttgcattagt tggcggcctg
     3121 ttagggatgg aacccgatga tgaacgttta gctgattctt tgaaattggc atatgaagcc
     3181 ggtatggaag tcttgtttgt tcctaagagt gaaaaagccg aacatccaaa ctctgtaaag
     3241 atgcttctaa aaaaaggaac acgaaagctt tcagtcactg gcatttcgat cggcggtggc
     3301 aatatccaga tttctgaatt gaatggattc aaaatttctt taagcatggg tactcctacc
     3361 ttgatcgttg tccaccaaga tgttccaggt atgattgcta aagtgactag tatactttct
     3421 gagaccaata cgaatatcgg cactatgaca gtgacacggg aatcaaaagg tgaaaatgcc
     3481 attatgataa ttgaagtaga tgatcctcaa gtagaagaaa cggtaaaaag actcaaacag
     3541 ctgccaaata tcgacagcgt aaattatttc gattaaggag ctaaaaaaat gtttttatcg
     3601 attgaagaat tagtagatca agcaaaagct tatccaagtg tagcagaatt gatgattgca
     3661 gttgaaatgg aaatgagtgg ccgttcccgt agtcacatca tcgagatcat ggaaaaaaac
     3721 ctctcagtaa tggaacaatc aatcaacgag ggaaaagaag gtgttacctc cgtcacagga
     3781 attaccggtg gtgatgcaaa aaaattagat gattatttaa atgaggcaga ctttttaagt
     3841 ggagaaacaa tcttaaaagc tgtacgcaat gctgtagctg ttaatgaagt caatgcaaaa
     3901 atggggttga tttgtgctac accgactgcc ggaagcgcgg gtgttgttcc tggtgtgctg
     3961 atggctgtcc gagatcggct tgatttgaca catgaacagc aattggaatt cttatttacc
     4021 gctggcgctt tcgggttagt catcgcaaac aacgcttcta tcagtggtgc agaaggggga
     4081 tgtcaagcag aagttggttc tgcaagtgca atggcagcag cagctttggt ttgtgcaaaa
     4141 aatgggactc ctcatcaagc agcacaagca gtagccatta cattgaaaaa tatgatggga
     4201 ctgatttgcg atcccgttgc aggcttagtt gaagtcccgt gtgtcaaacg taatgcttta
     4261 ggctcttctc aagcttttat ctctgctgat atggcattag cgggtatcga aagtgtcatt
     4321 ccgccggatg aagtcgttgc tgcaatgtac caagtcggac gtcaaatgcc gtcgattttc
     4381 aaagaaacag cagaaggtgg cttagctgtt acgccaactg ctaaacgact tgcggaagag
     4441 attttaaata agagggcgtg ataaagctcc ttaatttcga gtattaagag attagttatc
     4501 ttcataacaa aaaacgaaga gaggactaat tgcccttctc ttcgtttttt attaatccat
     4561 caatttcgta ttttttactt catcgatcgt ttttgttcct gcaagttgca tcgtgatcga
     4621 caattctttg ttcaggtgtt caaaaacaga tgttactcct tctgccccac ctagattcaa
     4681 cccataaatc actggtcggc caatcgcaac taagtcagca ccacttgcca gagctttgaa
     4741 gacatgctct cctcgacgga caccagagtc gaaaatcact ggtacacgtt tgttgactac
     4801 ttcggcaatt tttggcaata cttcaaagga cgcaggacct ccatctaatt gacgtccacc
     4861 atgattggat acccagatac catctgcccc tgcagagata gcgattactg catcttctgg
     4921 cgattgtatt ccttttacga taactggaag atgggtgatt tctttgattg ttttgatgtc
     4981 atctggtgtc aagccttgtt ttgcagctgc ataaatttct gcgattcctt ttccttcgcc
     5041 attaccactt gcactttgtt cgctatacgc agctaagtta ggcattggta atggaaattg
     5101 gaattgattg atgacgtctt cttcacggta accaccgagc gttgaatctg ccgttaaaat
     5161 gatagcttta gcccctgctt ctactgcttt gttcaaaatg aactcattga atctgtcgtc
     5221 tttactcata tataattgga aaaactgcgg tgcgcctggc gctgcatcgg ctgcgtcttt
     5281 gattgtcgta tttgcataag tgctgatgga aaagattgat ccagcagcag ctactccttt
     5341 agctgtatca gcttcccctt tttcatgtgc caatccttgg gccgctgatg gcgcttggat
     5401 aatcggtgta tctaattcaa ttccgaaaac agatgtatgc aaatcggctg agtcgattcc
     5461 gcgtaatatc cgtggcatga ttttctttgt attaaaagaa gtcgtatttt ctttcatcgt
     5521 ccactcatct tcagaaccac cgcgaatata gccaaaagct cctgcttcca tgcgctcttt
     5581 tacacgacct tctagttagg caatgtttac aatatcgatt ggatgttctt cattacttgt
     5641 atgataagtc atagtagatc cttctttcat cttcttgatt acatataaat catactaaac
     5701 ttacaaaaag taagcaaaca tttactctca gatactttta tcgctattct ttccaaataa
     5761 atcgacttac aagctgatct acttcttggt tttcatgaag catactatga gaggcatttt
     5821 cacctgtcac tactttttcc tctatttcat ttccgcgttg cttcaacaac gcatagaccg
     5881 ctaatgcact gtttaacggt actgttccat cactcaagtc tgtttcatct agttgaccag
     5941 caatcaagaa aaatcgagtg gttataggaa tattaccgat catttgctgg taatcttgat
     6001 agcggttaga aacgacagca ggcccctttg ctaactcatc tgtcagagac tgcgcactat
     6061 catcgacaaa atcattaaaa ggcgcgccaa ttgctgttaa tgtctgaact tgtggcaatg
     6121 acgtatcctg tccatacgtt cctaaatagc gtagtgcact aactcctccc atggaatgcc
     6181 ccactatgtt gactttatct atgccgtagt tttctttcag atacaaaaga caagttttga
     6241 tccattctgc ttgattccac tcgttatttt tattatcttc aaacaatact tggaccatcg
     6301 gattgtctgc ttttccagac aaatctcctg ttgcttgtat ttgtccatct gttcctactg
     6361 ttaaaaccaa ttcttttact gcttgctgat ttttttccaa acgccgcaac atccctttaa
     6421 aggaattttc tgtcccgctg tagccgtgga aaaaaagtgt tggaatagtg gttttttcta
     6481 tcactttact ggattctgcc gttttttctt tatcagaaac ttcagtagta tttatcttcg
     6541 tcgtctggca accacttaac gcaaacaaac tgataaggac taaagctgct gcagttgcac
     6601 gtttgcataa atttttgatt ggttctgtca tacacctctt cctttccata tagaaaaaag
     6661 aaagacttga aaggccaatc atatgatcga catttcaagt ctctttatta atcgatttta
     6721 tctacttgat cgaaatcaag ttctgtactg gtttcacgtc caaacatatc gatgttgact
     6781 ttcaattttt ctttttctcc gtcaacttct gttacttgtc cttctaatcc agcaaatgcg
     6841 ccttcgatga ttttcacgac ttcgccaact tctacttcta gatcattttg tcgcaggctc
     6901 ataccgatag aacgcaagat atgattgact tcttctggaa gtaatggtgc tggtttgctt
     6961 ccggctccgt gtgaaccaac gaatcctgtc acaccaggtg tgttgcggac aacataccat
     7021 gaagcgtctg tcatgatcat ttcaacaagt acgtatcctg ggaatgtttt atgaacgatt
     7081 tctttttctt tcccattttt gacttcagtt tctttttctt caggaacaac gacacggaaa
     7141 ataaagtcgg ccattttcat actttgcgca cgagattcga tatttgcttt tactttgttt
     7201 tcatatcctg aatatgtatg caagacatac cagttttttt caaaagattc cattgaaggc
     7261 tcctcatttc attgtaaaat aaaaaaacct cagtacaccc gaagtttttc tttcgcttca
     7321 ttatagcact aaaagtacgt tgtggctagc gagttatttt aaaatccagc caaataagct
     7381 ttggatggct gtatccatta cgtaaaacat caatccgaaa ataatagaag tttcgatgac
     7441 tactaacgta tctttacgga gttgttttct gcttggccaa gttacttgtt tgatttcatc
     7501 ttttacgctt tttagaaatt tcatgagaca acctcccttg ctactgttta tttcgtttct
     7561 ttatgtaatg tatattgatt acaatattta caaaacttat tgatttccaa acgttcgccg
     7621 ctttttcctt cactgactga ttttgtgtag ttgcgagacc cacaaacgga acaagcgagt
     7681 gctgcttttt tctttgccat ttacttctgc ccccttgctg agaatcagac ttatacttca
     7741 taacagtatc acgtaacttt ttacttgtca atttgtgaaa taaactatct attgttttgg
     7801 ataaaattat gatatgatta atttgaaatt aagctaaaat gagacaattg gaggtatagc
     7861 gatgctatta aaaacacttg tatataagaa aaaagatctg acaaccgtta cagagactag
     7921 tacattagaa gaagcattaa aaattttaga aacttccgga taccgttgtg tgccgatctt
     7981 agattcttca ggtaatattt tccgtggaaa catttacaaa atgcatatct atcgtcataa
     8041 agcaaatggc ggtgatatga cgcttcctgt cactcatctt ttgaaaaacg ctacaaagtt
     8101 catttacctg gatacttctt tcttcaaagt attctttacg atcaaagagc ttccttatat
     8161 tgctgtcctt gacagtgaaa atcattttta tggtattttg acacatagta cgttgcttaa
     8221 tatgctctct caatcttgga gcgtagaaca aggaagctat gtactgacaa ttgcttccac
     8281 aggaaagcaa ggagatttgg ctgctattgc aaaaatcatt tcaaaacaca gcagcattgc
     8341 cagctgtatt actcttgata tcgaaaaaga cgaatacatt cgacgtacat tgatcacgct
     8401 accggctaac actgaagcga aagtatgtga aacgatcgtc aaccacttag agaaaaagaa
     8461 ttataaagtg gtagaaattg aaaatttgca aaaccaagaa gcataatcaa aaagcgaaaa
     8521 aagaaaacag ctaatctgct ttcttttttc gctttttata attagacacg cttttcaaaa
     8581 tcaagcgcgc gatctaaata atacatcaat ggtgctgaaa cagccatgac gataaactca
     8641 cttaatgcaa gcgtgccata ggttgtccaa aatgcttggc cacctgttgg aactaacata
     8701 taagcaatca gtcccatgct gatggtaaag aagatcgtgt tcaatactaa acgcatcttt
     8761 acatttttca ctttgttttg caataaagct gtcaggccta aagaaagcaa tgtttggccg
     8821 ccaccaaaaa ccacatccat gataccaaaa ccaaagaagg cattatagac gatgaccccg
     8881 cctaaaactc cccaaagcaa tttacggtta aagacaacta aatgattcaa actttctgaa
     8941 atacgaaact ggatcggccc tgaggataca ggcgcaacta aaatagttaa cgctagataa
     9001 agagccatga tgatgccatt tacaacgatt acggataacc gttgtctgct tttcgttttt
     9061 tgcattttct ctcctctttt ctcctagttt ttttaacgcg ggatggttga tgaaccgcgg
     9121 ttatgtgaaa caaacaataa ttataacaga cttttcattt atttcaatcc gtcttatctg
     9181 cttttaaaaa agcaaaagct taaacaatgt aaacgtttgc taaaaatcgt gggatatggt
     9241 ataaagtaag tgtaaggggt cataagaatt cccagtaaag cagacgaagt aggatctgca
     9301 agaacagaat tgttgtgaaa cttaaataaa taaaaaagga tgtgggaatt tgagcaacct
     9361 cttactagag aacggtctag caagcagctg aagatctaag tggacatgcc gaaccttgga
     9421 acttctcctt acgtaagtaa tggatcatgc gcttgctagg cttttttgtg tttttcggtc
     9481 ttcggacctt tttcattttc ctagacttct gctataataa ccaagaatta attttgtaac
     9541 agattttttt gaaaaaacaa gtttctttca ttcaaaaaat agaaaaagct tgctaattat
     9601 tggttaacca gcaaacagga gtattaaaat atcttgtttt attgaatagc aggaggaaat
     9661 catgaaattc cattatcata tattgatcaa tccatcggca ggcagcggaa atggctataa
     9721 agtagcagaa agaattttac cggtattaaa aaataagcat attgactata cacttcatta
     9781 tagtgaatac aaaggacacg atgctgagat tgctgaaact ttggcaaaag aaacattgat
     9841 tccttggata gaagaagaac agaaaacaat cgatactttc ccccttttga tcatcgtagg
     9901 aggtgacggc accttacatc aggtacttga tactttctat cagatggaag tagaattccc
     9961 agttgcttat attccagcag gttctggaaa tgattttgct cgaggggcag gccttccaaa
    10021 aaatcccaaa aaaggacttc agctgatttt agcagctcaa tcaccagaaa aagtccacat
    10081 aatggcttat gaagaaaaaa taagtgaaaa aaaaggaata gctgttaata atttcggtat
    10141 aggattagat gcagctatcg tccatgcgac aaatcattct tccacaaaaa agcgattgaa
    10201 caaatacaac ttaggatcat tttcgtattt attctctatt ctccgtgctt tattcactca
    10261 aaaaggattt ccaattctgg tagatgcagg aggaaaacaa ttaagttttt caaacgcatt
    10321 tttatgtaca gcaacgaagc atcctttttt tggcggagga atcgcgattg ttccaactgc
    10381 tgatgcgtct aaaccagtca ttgattttgt cgtggtcgaa cgaatcaatt tctttaaaat
    10441 cttgtggctc attcttttac ttatacagaa aaaacaattg aaatcgaaat atttccatca
    10501 ctacacaaca aacagattac gtatcgtttc aacaacaccg caacatggtc aagaagatgg
    10561 agaagaaatg gaaaaacgac cttttgatat cacaataacc acaactaaac agttgttttg
    10621 gtgttaataa aagaggtttt gtcagaagct acatctggaa caccgtttat tcggaaaaaa
    10681 ggactgtgac acgacttttg tcacagtcct ttttttacta tgcagttaat tctttgccca
    10741 attgggttgc catcgatagt ttctttcttt acccaaaata aattggatca ttgaaaacac
    10801 cgcagcaaaa gcaactaaga cacaaccatt gaaccaaaaa gattctgcaa atgtttgatt
    10861 gtaactgagt gtttcactga tcaagttatt attttgaaca aaccaatatg ttggtgtgaa
    10921 tgccgctatt ttgctaacca tttctggcaa gtattctgct ggtacaaaca ccccaccgat
    10981 aaagcaactg cctgtgataa aaatatcttt gatcccaccg ctggtttctg agttttttac
    11041 caatgaagtg atacaagccg aaaagctaat gatcggcaat aaaaacaaga aagtatttaa
    11101 aatatgatac cataccgtta tatcccacga ggtgtggatc actaacagaa ccattagaag
    11161 taagaagcca ctgaacaaca gcagcgagta actaatcaaa ctaaaggtta ctttgcgatt
    11221 caagtttcgt ttggtaatgg gcgcactctg gtttcgttta ctgatctctt tgcgattaaa
    11281 cgctagattg atgacgctga aaccgctaaa aatggataga aacaacccat aagacaagaa
    11341 attaaaggtt gtgcccgtca acagcttttt catccgctga ctatatcccg cacttagctc
    11401 aacttctcca gtaaccgaga gtgccatttg tgtctgttcc atagctgcat cgattgtttt
    11461 atcttcttct tgaaacagaa tcagtgtttg caaaaaggtg ttcacttgct gtgtaacaac
    11521 tgttttagaa aaggcatctg ggcgactctg tatcttgatc gtcgccatct ttcctacttc
    11581 gacttgttcg gtaaagtctg caggcagatg taagataatg tttacacgat taaaataaag
    11641 agcatcatcg atttcttttt gtgaggttcc atccaatgta acgactgttt ccttgtcatt
    11701 caaatattta cgaaggccat cgactacagg cgactcttct tcactaatca aggcgatttt
    11761 cgcatcttgc agagaggtat tttcaacatc aatattttgc gcaaaaaaga atgtggcaac
    11821 aacagtgata attacaccta tcagtaaatt cgatttattt gctttaagca atgtccaaat
    11881 cgttttataa agcgtcataa cgcacccctc tttccatcca agcatttcct aacgcaaaaa
    11941 ggaaaataaa tccgatcaac catagaagat tggtataaaa actagccaaa tcagtgtaga
    12001 aataaagttt atacaaactt tcgctgatca agttgacgag atttaattga ccaacaatcg
    12061 gaatatgaag atcaatccaa tacttcactt gttccgaccc catcatacca gcaaaaaagc
    12121 tcatcgcaat cgtcactgaa atcaagatgc tatccttttg caccagattc atttttggta
    12181 tcaaattacc aaccaatgta cctaacaaaa tcgcacacaa cgaccccaga gcggtaacaa
    12241 tcatcaacca ttgccaacgt tcaccaaagt ctacttgata gataaaacga aagacagcta
    12301 acataatata aatctctgtc aaaacaatcg taaagcttgc tgccaaatta gcgattgaaa
    12361 ctaacgattg ctttctagga atcaaggaca agcgaatacc atttgacgat tgatttgctt
    12421 gctgatcctg tacattgcgg accccccaca tcgtcccgta gagaatcgcc attccaatca
    12481 gagtgaagaa ataaaaactt ttgaaactca tattacgacc tgcatgaact tcttgaaaag
    12541 atacttcttg ttcaaaaaca gtcatgatgg gcgcgagatc aacgttctcc acttctgcca
    12601 ctttactcga aattatacca atacgctgta aaaattgatc catgacgttt ttcatcaacg
    12661 tctgactcat tcctgcttga cccacatgga gcgaaacaga tgaaccatca atgagataaa
    12721 aaccagccaa atcatctgca tttagctttt cattcgcttc tacttccgat agttcctgta
    12781 cttcaaataa tggtttatca ttgacttcga cttgttctaa gactgtggta aaattttccg
    12841 aaagcgctgt ttgatccgct ttgattacaa ttccagtatt aatcgtttca agattgccac
    12901 cttgatcaat ctcgccaaag gccgccataa acatcaatcc taaaagaatt ggaaaggcca
    12961 atgtccaaaa caacatggat ttatttcgca gcagtatttt tgtgcgatat ttataaaaat
    13021 gccaaaacat tatttcccct cctctagtct ctcaaatcct tgccagtaat ctctaaaaag
    13081 acatcattca aggacgcttc tttggttgct atttttccgt aactgatctt ttcttcttgt
    13141 aagattgtaa gaatcggcaa caatactgta tttagttcag tagttgttac ttggagctga
    13201 ccttttttat aagcgattcc atcagaagct attgttcgct gaatcgctga caacacattt
    13261 tctgggagta atgggatgtc gatcgtcaaa cgatccgccg tccgtaccat atcttttaga
    13321 gattctttgg ttcctttagc aatcacctgt ccttgatcca tgatcacgat ctggtcgcaa
    13381 agaatttcta cttcttccat gtaatgtgtt gtatagacaa tggttgctcc ttgtcgattc
    13441 agctccttga tactttctaa aattttattc cgactttggg ggtctacagc tactgtcggc
    13501 tcatctaaaa aaattaaccg cggcttgtgg gcaatcccac atgcgatgtt caaccgacgt
    13561 ttcaagccac cactaagttc tcctggacga aacgaaacat atttttctaa tcccactaat
    13621 tgaatcacct cttcaatcaa ggctttcctt ttctcttttt cggaaacata taaaccgcaa
    13681 aaataatcga tattttcttg cacacttagt tcgtcaaaga ccgcaacgtc ttgaggaacg
    13741 accccaatca ttgccttgac agcataattt tcaggagtca ttgttgtccc gaacaattta
    13801 atggttcctt tgtcataatt taacaaagaa agcatacagt tgatggcagt cgatttccca
    13861 ctgccgtttg gacctaacag acctaagact tctccttctt gtacttgcag atcaaaatga
    13921 ttcaatgcta caaaatctcc atagcgtttg actaatttat caatttcgat aatcatcatg
    13981 aaaaactcct tccaaatttc ttgttgatct gactataata aagtgcaaga aaagaaacta
    14041 gtgacgattg gctaaaccac acatgacatt tgtcacaatg aaggagtctg ctatgcataa
    14101 atctcggcta ctcaacgaaa ctagctttct actggttatc tcattgcttt tgctggtaag
    14161 tcaaggattc gttgctatgc aagttatcta tttattgatc gccgttttat tcactggtgt
    14221 cagttattta ctgcctacaa agccaaattg gattttccaa gttattctat ttcttttatg
    14281 tgccttttta cctgatcttg tcctgtttct gccagcgacg atgcgagtga tcgcccctga
    14341 agaaagccac cttgattact tgtttttgtt aggttttttg ttgatttcga cggttggtaa
    14401 tcatcccccc atcaaggtcg cctttgtcgt aattttgctg tttttttcct tttacttacg
    14461 aaaaaaagat caacgaacga ccgatcttga gaaaaagtac attgtctcca aagatactca
    14521 ttgggaaaaa caacaagagt tatcggaaca aaataaagca ttggccctgt cacaagaaac
    14581 acaattagcc ttgcaaattg tggaagaacg gaaccgaatt gcccgagaca tccatgacaa
    14641 tgtcggtcat ctgctgtcaa gtgcgatttt acaaattggt gcgcttgaaa tgatcaatca
    14701 agaaccaaaa ctaaaagaac cattagaaaa tttaagtgcc accgttcaca ctgggatgga
    14761 tcggattcgt caaagtgtcc atgacctcca tgagacatcg atctcttttc aacactcctt
    14821 agagtttctg atcgctgatt ttccttcttc tgttacgatt gaaggaaact tgtttgaagg
    14881 attgaatgaa acgcagcaaa actgcttctt aatggtcatc aaagaagcct taaccaacag
    14941 tatcaaacat agtcatgcca aaaccgttgt tttacatttt cgaacgttgc cggcctttta
    15001 tcgcttgcaa atcacgaatg atggcaccag cccacaaaaa aattctgctc ccggtattgg
    15061 tttgaaaacg atgcgtcaac gtgttcaaga acaaaaggga caagtccata ttttccaaga
    15121 aaaccagcaa tttcagttaa cgatcatttt accaagggag ggacaccaat gataacagta
    15181 gtcattattg atgatgatcc atttgtgacg aaatcgttac aaaccatttt agaaagcacg
    15241 aaagagatcc gtgtgttagg cattggtcat tgtgccaagg atgcgctagt cttatacgaa
    15301 acccatcgtc ccgatgtttt gctgacggac atccgcatgc ctaaacaaac gggcatcgat
    15361 gctgccaaag aaattttggc taaatttccc aaagccatcc ttttgctttt gacgacattc
    15421 aaagatgaag aatacatccg cgaagcattt tccatcggtg tcaaagggta tttaatcaaa
    15481 cagaacttgc aagcgattat tccttctgtc aaatccgctt ataatggaca ggccgtattt
    15541 ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga ataaaccgac gattgatagt
    15601 catgcttatg cttcttttag tgaacgtgag cttgcaatca tcaaagaagt agctgccggg
    15661 aaaaacaata aagaaattgc agatgccttg tacttgagtg acggcactgt tcgcaattac
    15721 atcagccaat tactagaaaa gctagattta cgggatcgca cgcaattggc tatctatttt
    15781 tatcagcatt tgcaataagt cttatctaac acaaaaaaac ctgaatgatg ctccttcgca
    15841 tcattcaggt ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt
    15901 gaaagaatca aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg
    15961 gaaaaatagg cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt
    16021 ctacatcagg actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt
    16081 cattgccaga ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt
    16141 aacgcccccc ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta
    16201 accaacgatc atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa
    16261 aatcgtaggg agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca
    16321 cttctgctgt aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt
    16381 ctccaacaaa tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt
    16441 cattcgtccc actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg
    16501 tatagttccc atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt
    16561 tggtccggag agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca
    16621 tccggcttgc tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa
    16681 tcaacagttc cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct
    16741 gatattgatg gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga
    16801 ttgagcaaca ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat
    16861 ttgcatcaaa ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga
    16921 tacccgtgat ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt
    16981 ggaggacttt gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag
    17041 gtttccctgg attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc
    17101 cccgatacat tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt
    17161 tttccacaat tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac
    17221 gatctttttg attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa
    17281 gaggaataat actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa
    17341 tttgttcgat ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat
    17401 ctttgtctcc tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag
    17461 ccagccatgt atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt
    17521 tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac
    17581 tggcaggcag acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa
    17641 gtagaatagg aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact
    17701 ctgcaaaaag attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg
    17761 ttaaaataaa tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat
    17821 actgtatgcc gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat
    17881 aatggatttc ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag
    17941 aaaagtacaa actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa
    18001 caagttggat aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt
    18061 ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt
    18121 gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg
    18181 ctgcaatacc gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt
    18241 cttcgactag ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct
    18301 ggtagatact gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt
    18361 gatccatccc gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca
    18421 aaccggagat ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc
    18481 ccagcaattc tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg
    18541 aaagatacgc tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt
    18601 taagctgcct tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat
    18661 tttcttgaat atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg
    18721 tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa
    18781 tcgcggcaac tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc
    18841 ttcttctata gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat
    18901 actttcagtt catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta
    18961 ctattgaaca atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga
    19021 atggctgcag ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgtcgg
    19081 gcaaccgcat ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg
    19141 tgaaaaggag aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga
    19201 tatttctggt acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt
    19261 agcttatctg tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct
    19321 gtatcacgtg ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc
    19381 tttttcccaa atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg
    19441 ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt
    19501 tccatacgca gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc
    19561 agcttgtcat tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt
    19621 gcttcataca ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca
    19681 ttcataggga tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg
    19741 gttgtttctt ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta
    19801 tatactttat gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa
    19861 aaagcttcta aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc
    19921 cgttttagat gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta
    19981 ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat
    20041 aaaacgatat gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga
    20101 caatttgatt tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt
    20161 acgttagtga agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg
    20221 aaaaaacaac ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta
    20281 aaaactgcta gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat
    20341 cttcttcaaa ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa
    20401 aggtgacatg gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac
    20461 acgctgacgt tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga
    20521 ataactctgt ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt
    20581 ttcttgtggc accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg
    20641 gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa
    20701 attggtcaaa tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac
    20761 tccttctttt gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc
    20821 attcaagaag ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg
    20881 ctgcgcaata gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg
    20941 aacgaacatt ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc
    21001 ttttttggaa aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt
    21061 tacttcttct tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg
    21121 tcctcggtca atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga
    21181 tgctcacttt ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa
    21241 ggagaagatt agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca
    21301 attgctagag gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc
    21361 ccgagtactg gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta
    21421 atgacgatta ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc
    21481 atgcatactg atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt
    21541 cttactcctt caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa
    21601 gggttgcaga tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa
    21661 gcaatcgatt ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct
    21721 gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc
    21781 cgcttaatca ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa
    21841 gaattgaacg taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat
    21901 aaacaaaaaa acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca
    21961 taaaatagcc cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc
    22021 tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta
    22081 gcatcgggat taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc
    22141 ctatgacaaa ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga
    22201 tccccgtaac agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa
    22261 atccatgatc tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg
    22321 ttccagcatt accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt
    22381 cacttaccaa gtcttcatag tgactgatta aagaagcggt cgacatacct aaaaacaaca
    22441 gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt
    22501 ccacattgac cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga
    22561 tgatatcatc gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag
    22621 ctaagaagtc ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa
    22681 ctgaaagcac tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta
    22741 ggtcgcgcag agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat
    22801 agattgtttc agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt
    22861 gattggctac gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt
    22921 aatggagaag ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag
    22981 caatttgctt tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag
    23041 aaagcatatc agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt
    23101 tgtcttcttc aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga
    23161 tcagctgcct gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca
    23221 tctctaaaaa actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact
    23281 gggcaaatct ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc
    23341 ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa
    23401 ctcttctgta aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat
    23461 tcccagatcc attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga
    23521 aaaatgaact cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat
    23581 ttctgttagt cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac
    23641 cattcgcttc aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc
    23701 cgataaatct gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc
    23761 acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaagcc
    23821 agatgtatcc cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt
    23881 tttataataa gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat
    23941 agacgaaacg cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc
    24001 gatcggtgtt tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt
    24061 taccttatca tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat
    24121 ttgaccgcct tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa
    24181 aaaatatttt acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat
    24241 cctcactaat aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa
    24301 aatggatccg ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat
    24361 tcaattgatc aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt
    24421 catcatgggc aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag
    24481 caatctctgc caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt
    24541 tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt
    24601 caaacagctc gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg
    24661 ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa
    24721 catccagcaa gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt
    24781 cttccagctc gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca
    24841 agaaacgcac atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt
    24901 ctccgccgac tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt
    24961 ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga
    25021 tcgctacttt catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag
    25081 tagacatcat ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct
    25141 tcacggatac tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt
    25201 cgttcactca tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc
    25261 gttgcccaga aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca
    25321 ccgctgatca gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct
    25381 tttttatttg tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg
    25441 atctcaacaa cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata
    25501 tcagacattt cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca
    25561 atactatcga ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt
    25621 tcacggaact gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca
    25681 tatggtgcta aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca
    25741 ttcattgttt attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac
    25801 caaaaaaaga aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt
    25861 gaaatcgaat ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat
    25921 caattaacag ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa
    25981 cagctgaaga aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat
    26041 acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa
    26101 gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa
    26161 ctttctgatt tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa
    26221 cgagctgaat tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat
    26281 ctacacgatt ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt
    26341 ataaattttt taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt
    26401 atgcttgaag ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt
    26461 tacttttttc tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct
    26521 attttggggg agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac
    26581 tgtttcgaaa tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca
    26641 aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc
    26701 atgcaacaaa aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg
    26761 acttattctg cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc
    26821 cgttttttga tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa
    26881 cttgctgaat ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt
    26941 cagtctgact tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc
    27001 atcacaaaac acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt
    27061 ttagtagaag taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca
    27121 gataactcat atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat
    27181 tcaagaacta gccatttaca cttgctttct aattaaaa
//
LOCUS       DAJSMI010000018        26979 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJSMI010000018 DAJSMI010000000
VERSION     DAJSMI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.018"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(135..2288)
                     /locus_tag="ON430_000659"
     CDS             complement(135..2288)
                     /locus_tag="ON430_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCR3218602.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
     gene            complement(2582..3430)
                     /locus_tag="ON430_000660"
     CDS             complement(2582..3430)
                     /locus_tag="ON430_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3218603.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3654..5594)
                     /gene="thrS"
                     /locus_tag="ON430_000661"
     CDS             complement(3654..5594)
                     /gene="thrS"
                     /locus_tag="ON430_000661"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR3218604.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5931..6110)
                     /locus_tag="ON430_000662"
     CDS             complement(5931..6110)
                     /locus_tag="ON430_000662"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCR3218605.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6157..7980)
                     /gene="cadA"
                     /locus_tag="ON430_000663"
     CDS             complement(6157..7980)
                     /gene="cadA"
                     /locus_tag="ON430_000663"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR3218606.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8258..10537
                     /locus_tag="ON430_000664"
     CDS             8258..10537
                     /locus_tag="ON430_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3218607.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10630..11277)
                     /locus_tag="ON430_000665"
     CDS             complement(10630..11277)
                     /locus_tag="ON430_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218608.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11456..12175)
                     /locus_tag="ON430_000666"
     CDS             complement(11456..12175)
                     /locus_tag="ON430_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3218609.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12306..13457)
                     /locus_tag="ON430_000667"
     CDS             complement(12306..13457)
                     /locus_tag="ON430_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCR3218610.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13674..14414)
                     /locus_tag="ON430_000668"
     CDS             complement(13674..14414)
                     /locus_tag="ON430_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3218611.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14418..14741)
                     /gene="rsfS"
                     /locus_tag="ON430_000669"
     CDS             complement(14418..14741)
                     /gene="rsfS"
                     /locus_tag="ON430_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR3218612.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14798..15391)
                     /gene="yqeK"
                     /locus_tag="ON430_000670"
     CDS             complement(14798..15391)
                     /gene="yqeK"
                     /locus_tag="ON430_000670"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCR3218613.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15375..16025)
                     /locus_tag="ON430_000671"
     CDS             complement(15375..16025)
                     /locus_tag="ON430_000671"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR3218614.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16035..16346)
                     /gene="yhbY"
                     /locus_tag="ON430_000672"
     CDS             complement(16035..16346)
                     /gene="yhbY"
                     /locus_tag="ON430_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR3218615.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16362..17471)
                     /gene="yqeH"
                     /locus_tag="ON430_000673"
     CDS             complement(16362..17471)
                     /gene="yqeH"
                     /locus_tag="ON430_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCR3218616.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17468..17995)
                     /locus_tag="ON430_000674"
     CDS             complement(17468..17995)
                     /locus_tag="ON430_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCR3218617.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18175..18960)
                     /locus_tag="ON430_000675"
     CDS             complement(18175..18960)
                     /locus_tag="ON430_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR3218618.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18953..19822)
                     /locus_tag="ON430_000676"
     CDS             complement(18953..19822)
                     /locus_tag="ON430_000676"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCR3218619.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19823..21202)
                     /locus_tag="ON430_000677"
     CDS             complement(19823..21202)
                     /locus_tag="ON430_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR3218620.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21210..21629)
                     /gene="fabZ"
                     /locus_tag="ON430_000678"
     CDS             complement(21210..21629)
                     /gene="fabZ"
                     /locus_tag="ON430_000678"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR3218621.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21654..22130)
                     /gene="accB"
                     /locus_tag="ON430_000679"
     CDS             complement(21654..22130)
                     /gene="accB"
                     /locus_tag="ON430_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR3218622.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22133..23368)
                     /gene="fabF"
                     /locus_tag="ON430_000680"
     CDS             complement(22133..23368)
                     /gene="fabF"
                     /locus_tag="ON430_000680"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR3218623.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23387..24124)
                     /gene="fabG"
                     /locus_tag="ON430_000681"
     CDS             complement(23387..24124)
                     /gene="fabG"
                     /locus_tag="ON430_000681"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCR3218624.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24136..25053)
                     /gene="fabD"
                     /locus_tag="ON430_000682"
     CDS             complement(24136..25053)
                     /gene="fabD"
                     /locus_tag="ON430_000682"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR3218625.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25160..25384)
                     /locus_tag="ON430_000683"
     CDS             complement(25160..25384)
                     /locus_tag="ON430_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR3218626.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25437..26399)
                     /locus_tag="ON430_000684"
     CDS             complement(25437..26399)
                     /locus_tag="ON430_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCR3218627.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26409..26849)
                     /locus_tag="ON430_000685"
     CDS             complement(26409..26849)
                     /locus_tag="ON430_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3218628.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         7365 a         5986 c         4689 g         8939 t
ORIGIN      
        1 ttttttgccg agtaaacggt gttcaaaagc cagtttctcg gtatgaagtc aaatttaagc
       61 aaaaatgtga gaagctattt tcacaaaatt tttcttcata ctcgaaacta agcggctttt
      121 tcacgacctc ttttttattt tgccatgaac atatcataat acgcgtccat tatttcttta
      181 gcaatcgtca agttcatgtg gtcttgttcg tctaataagt tcggcaagac aacactgatg
      241 gcaatttccg gattgtctgt tggtccgtat gccacgatat tactattgac tgtcgtaata
      301 tctggatgac cttccgcaac cgtttctgca gtccctgttt ttgcggaaag gtccattttc
      361 gcactggcta atggtttggc tgctgtgtaa ggatcggtac catggacaac ttggtggaat
      421 ccttcacgga ggatcgccat attttctgca gaaatattga ccgtattttc tactgttgtg
      481 ccaatttctt tcttcacttc acccaagttt ccattttcgt cattgccata gatcgctttg
      541 actaggtgcg gctgtactcg tttcccgcca ttagcgactg ttgaagcata ttgagctaat
      601 tggattggtg tatatgtgtc aaactgtcca aatgccaaat cggtaaagtt acctggagta
      661 taccattctt tgccattatc cgcattgaac ttcttcatat aatcaacaga acgtgagatc
      721 ccaggagatt cattcggcaa atcgatccca gtcgttgtac caagaccaaa ctctttgaaa
      781 gagtctctca attgctgata agctttagct tcttcatcta aactaggtag tcccattcca
      841 ggtgtatagt ctagtcctaa catctttaac gccactttga tcatatacgt attagaagac
      901 aattccaatg ctttgactgc atcaagagca acttgtccgc ttcgattaaa gacagagctt
      961 ttttctgaag caccttgcag acgaatcggc tcatcaatca aaacttgatt tccgctgatc
     1021 gcgttacttg cccagcctgc tgtcaaagta ccagccttga ctacagaccc tggagcaaag
     1081 gcgctagtaa tcgtgcctaa agcattctcg gtgatttctt tggaaccttg ttcatgactg
     1141 aaaccagtca ttgctagcac ttctcctgtc tgaggattca tagccactgc ataagcacca
     1201 ggcgaatact gtgctttgcc gttgttgatc aatgtttgat aattcctttt caaaatttct
     1261 tctacttttg attggaattc tgcattcata gataacatta aattagaacc tttttcccca
     1321 gagaagattt ctttttgatt cgaaacattt ccttcattat caagggaaac ttcatattgg
     1381 gttttggttc cttgcaggac atcttcatat tgtttttcca aataactttg tcctacacgg
     1441 tcattccgag aataaccttt tgctaggtat ttctctgctt catctgctgg aagtccttgc
     1501 ttttcagtcg tcacactgcc aagaatactt ttcagtgaac ttgctgcatt atattctcgt
     1561 gtccagtctg tgccagtcga aactcctgga agttcagaag cgcgttctgc cacaacagct
     1621 aactcttgat cagtaacacc agagtttttg atatagacag tgtttaattc atatgcacta
     1681 ttcatccgtt taaagatggt agcaattttt aattgctcct cgtttaaatt aatctcatca
     1741 gctgtgactt tattgaccat cgcactatat tgttcgctgt tccctaattg cttttctttt
     1801 gccgaaagtc tttttgtggc ttctttcaaa tgtttgggat cagccagcca aaaatctttt
     1861 ttatctctat ctgtcagatt ttcatctacc ggaacatcga tcagcgagtt cagcttcgtt
     1921 gcgatttcaa gcaaatcttt agccgtaact tgtgttcctc tcgtatatgt gatggcctga
     1981 ttcgctttat tcgtaactaa agcttttcct gtggagtcgt agatcattcc gcgaggagta
     2041 ctttcttgga ccgtaatcgt agaagtagac ttcactcgcg cctccatctc ttcgccgttc
     2101 acgatctgca aataacccaa tctagcaatc aacgcaacaa ataacgtaaa tataatgaaa
     2161 aaaagaaaat ttaagcgaaa aggaacatgg gaccggcgaa acgttttgtt cttaggttct
     2221 tgtgcacctt ttcttatttt ttctaaccaa gaagtattta atattttttt cataaaatct
     2281 ttcatcattt cctatccttc cgcctattta cattcatgct tcattgtacc caaaaacttc
     2341 ttaataaaaa agaaaaaatg ggcggagcaa gaaattcttt acctaatttc aagataaaat
     2401 caactttttg tttcacagca atcacttaaa gttacatatc caactatttt tttcattaaa
     2461 aaataaactt atttttgaaa attatgtttt cttaagtcgt gaaacacttt gatggatcat
     2521 ttatttgtgg aaaactacgt tttgttattc taaaaaactt atataaaaaa tagtaaggtt
     2581 tttatttcga atccataaaa tattcttgga ttgcttctcg tgtttttttc actttttcgc
     2641 taagcttttc gtttttagcc atatagaaat aaaaaatgat atcgatcgta gcaaattggg
     2701 ccaaaagtga attagttgcc gcacttcgat tcgtgatttc ttttggtcta gatgtttgta
     2761 cgagtacatc ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt
     2821 cgatattcat gcttttcgcc agctcggcta atgcgactac atctgcactt ctacctgagt
     2881 ttgaaacaag ccaaagcact ttctttttct cattgctgac aagttgcggg agcaagacgt
     2941 ggatatcttt ttcaaaaatg attggcttac cgacacgaga ccatttttgc agaatatctt
     3001 ctactactaa agaagaggcg ccaaccccat ataaaaaaac agtctctgcc gattccaaac
     3061 actctacgac tttttggatc gtttcttctt ctagaatatc tactgtttct tttatcgcta
     3121 cttgtgcatt gttaccaagt ttgtttttga tagagtaaaa ggactcattt gcttctatat
     3181 cactaaaacc ttcatcgatt ccagtctgta gttctgttga aatcgtgatt ttgaagtccg
     3241 tcagactttg aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg
     3301 aagcttctgc taattcatta gctgtcattg tagggacctt ctcaatatta ccgataatgt
     3361 agttggccaa ctttttttct gctgaagaat aatcttccat catactttca atacgactaa
     3421 ttacgctcat aagttcctcc acccttttaa tgatagtcta tgtaagtata ccatatcaga
     3481 cccttattca cggtcgtata ctggataaaa aacaaaaaaa caatccgccc aacagaatat
     3541 cttatcccat caatcccaca gattctattg agcatctgtg tctttgagga atacctgtta
     3601 tctattgttc ggattgcttt ctatctacta cttttttaca ggaaatttac ctattagttt
     3661 tctctgctgt aatttttgac ttctgcttcc atagcatcaa taaagatatt caaatcttct
     3721 acagctgttt ctttacttcc ataacgacgt acgttcactg ttgcatcttc caattccttg
     3781 tctccaacaa ctaattggta agggatcttt tgtgtttgag aggcacgtat cttatagccc
     3841 attttttcat tacgatcatc aacttctaca cgcaacccct tcatttgcaa acgttctttg
     3901 atttcataag cataatcgcc atgtgcatca acagaaacag ggatgattgt tgcttgtata
     3961 ggtgccaacc atgttggaaa agcgcctttg tatacttctg ttaaataagc gacaaaacgt
     4021 tccattgtag aaacaattcc gcggtggata actactggac gatgcgtatt ttccccgtct
     4081 tctccaacat atgtcaaatc aaagcgctct ggcaataaga agtctaattg tatagttgat
     4141 aatgtttctt ctgtaccaag agctgttttg acttgaacat ctagttttgg tccgtaaaag
     4201 gctgcctcgc cttctgcttc aaagtaatct agttctaatt catccatagc ggctttcagc
     4261 attgcttggg ctttttccca cattgcgtca tcatcaaaat atttatctgt attgtttgga
     4321 tcgcgatagc tcaagcggaa acgataatct gtaatattga agtcagcata tactgccacc
     4381 atcaattcaa gcgtacgttt aaattcatct ttgatttgat caggacgaac aaatgtatga
     4441 ccatcattca acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc tgatttttca
     4501 tagcgatgca tcattcctaa ttcagcgata cgaattggta attcacgata agaatggatc
     4561 gtgtttttgt agaccatcat gtgatgaggg cagttcattg ggcgaagaac aagcatttcg
     4621 ccatctccca tatccattgg cgggaacatg tcttcttgat aatgatccca gtgtccagat
     4681 gttttgtaca actcaacatc tcccattatt ggtgtatata catgttggta acctagacta
     4741 acttctttgt ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc
     4801 cagaatggca aaccagaacc tacttctggg gaaaccatga acaagtcaag ttcttttcct
     4861 agtttacgat ggtctctttc ttttgcttct tcacggagac ggatatattc tttcaaagct
     4921 ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca tttgattgtt tgagtttcca
     4981 cgccaataag cacctgccac tgataataat ttaaatactt gtatacggcc agtggatgga
     5041 acatgtggtc cacgacacaa atcgatgaat tcgccttgtt gataagcagt aatcacttct
     5101 tcttctggca attcactaat cagttctact ttgtacggat cacttgcaaa aatttctaat
     5161 gcttcctgtt ttgaaagaac acggcgttcg atcggcagat tttctttgac gattttcatc
     5221 atttctgctt cgattgccgg caaatcttct gcagttactt ggttttgtcc attatctgta
     5281 tcgtagtaaa accctgaatc gatagctggt ccaacgccaa agtggatatt tggatagagg
     5341 cgacgcatgg cttgggccat caaatgagct gctgaatgac ggactaagcc aagtgcatct
     5401 tcatggtctg gtgtgatgat ctcaatactt gcatcttcct cgattggacg agtcaaatca
     5461 acgagttttc cgttcacttt accagctaaa gctttttttg ctaagctttt tgaaatgctt
     5521 tcagcaattg ccaatgttgt tgttccagac tcaaattctt tgactgcacc atctggaaaa
     5581 gtaattttta tcataatagt ttcctccttg aaatgttttg attgatcaga tcatcaagct
     5641 gaaaaacaaa aaaatcctag catctcttga aaagagatac taggacgaaa agttcgtggt
     5701 tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt
     5761 gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa agtttcagcc
     5821 tagcttttcc tctcttatcg aagactctca aattcagttg gctttctcat caatcaatta
     5881 tgtgtttatt taaccactaa taaatagaaa aatcaagtgg gaaactaaac ttattttttc
     5941 cattccccat ttacaccgta tgttcctttt ttcatctcat tgatgatgac atggatgtgt
     6001 tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga tatctttcat catattttct
     6061 aattgttccg gactgcgacc ttctactaat tcgacatgta cgaatggcat aatgctctct
     6121 cctctttctg atcgttcttt tttcaactcg attctatcat actatgtttg tcttgggtac
     6181 tgaatcagga aaaatggctg gatctttttt tgctctcaag gcactcaaaa tagagaccgt
     6241 atccacgact tcttgcaaaa cagctccgaa taatgcgggg attagtcccg tacttgcaat
     6301 taacatcaaa aaaatacaaa taaagatgcc aatcagtaca gactgtttcg ctattctcat
     6361 cgtatctctt gaaatcacta ctgcttttgc tacacgcaag agatcatctt tcaagatgac
     6421 cgcatctgca ctttcactag cagctgtaga accatgagcc cccattgcaa taccgatatc
     6481 tgcagcagca agtgctggtg cgtcattcac gccgtctcct accatcgcaa caggtctttg
     6541 atctttaggg atactttcta aaatggtcag tttatcttga ggcaagctac gggcatgtac
     6601 agaatctaat tggacttttt ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat
     6661 catcatcaaa tttttcacat caagttttct tagctccttg attgtctttg ctgcttctgg
     6721 gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg ttacatagat
     6781 tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc caatctttac
     6841 ttcatgatta ttgactactg cttggattcc ttgaccggtc acttctttca gttgactaac
     6901 aggatacagt tggctgtttt tcacgttgtt cactaacgaa cgagctaaga tatggctaga
     6961 ttcctgttct gcactagctg catacagaag tagctcttct ttttttattc cattgactgg
     7021 ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt cctgttttat caaaagcaat
     7081 cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt tttacaacga tcccgttttt
     7141 acttgaacgg ctcattcctg caactaaagc aatgggcgca gctagaatca gtggacaagg
     7201 tgatgccact accagtacct cagcaaatcg taaagggtct tttgagaacc accaagaaat
     7261 accaccgatc aaataagcaa tcaacgtgaa agggactgca taacgatcag ctaaacggac
     7321 aaatcgggca ggcttttctt ttgattcttc gactagttta ataatcattt gatactggct
     7381 gtctgcggca cgtttttcag cgatcatttg gatcgatccg tctccattta aagaccctga
     7441 cataatctga tctccaggtt tcttggtttc cgggcgggac tcacctgtca aagaagattc
     7501 atcgaccgaa gattctcctt tggtcactcg accatctgcg ggtacaagtt cgccaggctt
     7561 gactagtaac tggtcatcaa tatttatttc atcgattact atatcctgca attttccttc
     7621 tttgaatcga tgggcaattc ttggggtgtt atctaacaaa gactgcagtt ctttacccgc
     7681 tcgtttattc gcgtaatcct ctagactatc tccgcccgta agcatgatca atatcatcaa
     7741 actagcccag tattctttga cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc
     7801 tacgccatat cttcctgaac gtaacgtctt gatcatttcc aaaaacatca agaaggcgag
     7861 aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc
     7921 acttaataaa gctatcccac caataagcaa agtaagtaaa aattttttga aatgtgtcat
     7981 tgttttttct cctcttttat gtgacttaga gaggttagta ataaatttcc agctgcctct
     8041 ttcatcttaa gcatagtcct tgactagcac tttttaaaac aataggcctt aattgaaaac
     8101 aactatcact atcagcgttt ccaaaattca tcagttctac aaacaaaaat attcagcaag
     8161 gattactttt tcgattcatc agttaaaaag tacctttttt cgaaaaacta tgctatactt
     8221 ttaattcgtt atttacggcg gaaggagcaa acatgcgatg aatgagaaac aactagaaca
     8281 aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa caaaatatgc tggatacaaa
     8341 acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa gacacagcta atcacaagat
     8401 tcgagctggt tccaatgaag ctttttatga atctgctgtt gaatatcgcc aacatgagca
     8461 ggaattgctt ttgaaatacc atactcttga agcacagcaa aaacgcctgc aaacgttgtc
     8521 tgtcatgaaa gatagtcctt attttgcccg tatcgacttt aaagaagacc gtgaaacaga
     8581 aacattatat ctaggtatcg cttctttaag agatctggca gaagaaacaa tcgttattga
     8641 ctggcgcgca ccaattgcca atctttatta tgaaggtgag attggtcccg cattttatga
     8701 aactgaaaca gatagaatag atgtcgatct gcagttgaaa cgacaattta aaatccaaga
     8761 aggacgtatt ttatctatgg tagatacgtc ggaagtaatc aatgatgact ttttgctaga
     8821 gatattggat gaatcatcaa gcaaccatat gaaaaacatc gtctctacga tacaaaaagc
     8881 acaaaatgca atcattcggg acacaacaag taaagtaatg ctgattgaag gaatcgccgg
     8941 aagtggaaag acatccgctt tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa
     9001 gtggctcgag gctgaaaacg ttcttctatt ttcacccaac catttgtttt ctgattacat
     9061 ctcgactgta ttgccttcat taggtgaaag cggcgtgcct acacagacat tcaaaaatta
     9121 tttggtgcag ctgctgccag agttttcttt actagatgaa cagcaacaag aaagtggttt
     9181 tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc ggattgacct tagtcgatca
     9241 gattgatcgc tatatccaag gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat
     9301 aaatggccgt acgatcctat caaaggaatc gattcgccaa tggtataaag aaacaaatga
     9361 acaattacct ttacatcata gactttctct attacaaacg aagctcctga aaaaattagg
     9421 cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg aagaacagct
     9481 ccaagaacta tacgcaacag atccaaatct tgaatataca gaacaaaagg agcgttcatt
     9541 acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc atcgagggat
     9601 catgaattat caatttgtca atataacgaa acaatatctc cactttctac aaactgtgcc
     9661 actttctctt ctaacacgac ataaaatcag cgagaccagg tggcaggaac atttgacaac
     9721 tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac tattcttttt
     9781 actgatgcgc cgaacatatc cagtgacggt tacacacaaa gctcgctata tctttattga
     9841 tgaaatgcag gatttcgcac catcacaagt ggcattgctg caagccttgt tcccaaaagc
     9901 aaatttgact ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg
     9961 atcattggaa gaccttttcg tggaccgtga agtcactcgt ttccaattaa cgacaagcta
    10021 tcgctccaca aaagaaatca ctgattttgc taatcatttt attgtgaaag aacatcaagt
    10081 agaaacaaca gcaagaaaag gagaccttcc tttgctcatc caaggaacaa cagatgatat
    10141 caaattgaat tggttagcag ataccattcg aacgactcag ggaaaagccc ggtattggcg
    10201 tacagcaatc atagctaaaa cacaagaaga atgcgaagct ctttatgctc aactgccaga
    10261 cgagttgaaa caacaagtcc agctgatttt aaatgagagt gactttatga agcgttccat
    10321 tattttactt ccagcctttt tagcaaaagg tctggagttt gaccgagttt tcttgtggga
    10381 tgtagatgca gaacattttt cttctcagca agacaaattg atcctttaca ccatgtgtac
    10441 acgtgcgatg catgaattga ccctttttac ctccaataat gaaagtcctt tgttaaacaa
    10501 tatggatatt tcatcttatg agaaaaaagt actgtaagta aaaagacaac tgctatcatt
    10561 aatgttagca gttgtctttt tatttacaat aataattgca gaaaaagtaa ttcaatcatt
    10621 taaggtcatt tattctatat ataaatccaa atctgtttca ggatatttta atttgacaag
    10681 cgaaataatc attttcagtt cttccatgtt ttctggatat tctgggtcaa ataccttgct
    10741 atgacggcca atcccgccat tgtcgatcaa tcggtaaatt cgattccatt caatgaattg
    10801 gtcaattgat tggaaattgc tgattggata gccgccacaa tccaaaatct ctgatacacg
    10861 aaatgctagg tatgcactat cgatcacttt attttttttc gtgtctatct tctcatttct
    10921 ttcgataaga attttttcag gacgatgttt caagctccac tctaatagta gaaagaaagc
    10981 ctccatccga tactcttctt caaataaccg aaaggctgaa tcagaacgac gaaaaagcag
    11041 cggaatcaga taattttgtc tataaatcag cattggtgcg cggtccacca tacactctaa
    11101 ataacggttc agataccctg taccgacata gtttcctaat cgatagcctt caacgccgaa
    11161 tcttgcgtat ttttgcgcta attttgaaca atcagttacg ttcacagcta actcttctac
    11221 tttgcggtaa ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg cttccatatt
    11281 cttccccgtc catcttccac atacttcata tcatttcaca tgatacttat gctctcatca
    11341 taccacaggt tgaattagaa aagaaaataa atcacacaaa aaagcaagac aatctgttag
    11401 tgagattgtc ttgcttttcg aaaaagactg aatacttttc gttaaaactg gttgcttagt
    11461 cttctaatgc cacattatgg aacacatgct gtacatcttc tagatcttcc agtgcatcaa
    11521 tcattttttc aaatgtttct tgatcttctg gagatagtgt tacctcgttt tgagggatca
    11581 tttggatctc agcgattgaa aaatcagtga ttcctttttc ttttaatgct tcttgtatac
    11641 tatggaaatc cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc attacatcgc
    11701 gaacatcgat gtctttttcc attaggtatt caaggatctc gtcagcgtct tctcctgcaa
    11761 aagcaaagat tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca cccatgttcc
    11821 cgccattttt cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca ttgttcgtca
    11881 atgtatcaac aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc aattcagagt
    11941 atgtttcatc tcctgcacct tttgcttttt cgattgctcg gtcaataata tgttttggta
    12001 cattataagt tttagcgcga tcaatgacga aacgtagttt ttggtttgca tgcggatcag
    12061 gatcacctga ttttgctgcc gcgtagattt caatcccaaa ttttgcataa atccggctat
    12121 tattagcatc tttagcagtt ttctttgcta cgatatttgc ccatttacgt cccatatttt
    12181 cacttcgctt tctgatttga tagtttcata acgcaaaaac gtcctgttcc attatacttg
    12241 tgaatcacga ttctgtgaag ctttttttct ttcttccttc attcttctca tttttagtca
    12301 attatctata ctcggattgg gatttgcccg aaattctgtt ccggaatatt gcctcccaat
    12361 tgatagattt gatcacttct aactaacagt tccgcttctt gtgcctcttt ttttccgatt
    12421 cgggaataaa tagaaatctc ttttttttct tttaagaatc ttcttccagc atcggtaaat
    12481 ccaagtagtc gaagataatt tttattttgt tggacaggaa tctcttgctt tttgatattc
    12541 agcaagacat aacaagataa tcgttggatc cgtgtccatg tatatctttt tgtttttacc
    12601 tttgacacaa aatcctcaaa actatcagca gttgcagctg ctgttttcat gcgcgattct
    12661 attccttcgg tcatctgata gatatctgtc agttccagca aagtagaaga taacaaccga
    12721 taacgcaaat acggccaata agattcccaa gtctgcagcg aacaagttga aagatcttct
    12781 tctgtctgag gcggcacaag tccagctatt ctttttcctt cttctaacgc atggcgaatg
    12841 gctgttgcac tggctatcct ctgatctgtg atgatttttt catgatagcc tgcactttta
    12901 cgtcggatcg gatgtaattc catagggtgg acatacttgg cattttcctt tgcataactt
    12961 aatcccaaga tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata cagactgcga
    13021 aaaacctcag tcattttttg cggataacta tacgtttgat cagacagttc ttgaaatgcc
    13081 tcttctatcc tatcttgatt ctcttggaca aagcgtccga aactgctgta atcaaacgaa
    13141 gtctcttctt ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa gatttttact
    13201 gcacctttag caaaatagtc agctgactga accgcccatt cgacgggaag ctccactact
    13261 aaatccacac cattttgtaa agcttcattc gcgcgatgcc atttatcgat gatagccggt
    13321 tcgcctcttt gcaaaaaatt cccgctcatt acagcaatga cgacctctgc tcctgttatt
    13381 tttcgtgcca tttccacatg atagcgatgt ccattgtgaa aaggattgta ctctacgata
    13441 atcccgcagc ttttcatgcg catcctcctt catagttatc gataaaaaag aaatagagac
    13501 aagtaaaaaa aggcaaaaat cggaaaaaat acccgaacta tataaactat tctcgcaaac
    13561 acaatacttc tgcgacgaaa agctgcactg caagagcaat gcctatatcg ttcggatata
    13621 taatttatgt tgaagattta ttcttttctt gttaactcga gcaggcaaaa ccgctagtct
    13681 ttgtatgcta caaagaacca gcgctttgat ttctctgatg ctgcttcgtc tgtaaaatca
    13741 gaaaatgctt cgactttcga aaagccgctg ttttccagca ttcttaaata actttccaat
    13801 ggataggtac gctcttgatg tagttcatct tcacgaataa atcgaccaga cccttcttgt
    13861 tcttcaacga aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc tggataacta
    13921 tcccacaaaa aagcaaaatt atccccttga taatgatagc tatattctgg aaaaacctca
    13981 tctacttgat aggtggagtg tacgtcaaat aaaaatcggc cattttcttc taacgcttga
    14041 tagacttcat caaacacttg ctgtacctct tgtctatttt tcatatagca cagcgaatca
    14101 gaaaaacagg taattgcctg atattgtccg atttctgata aatcgagcat attgccttcc
    14161 acaaattgga tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat caacatctct
    14221 tctgaaaggt ctaaccctgt cactttatat ccatcttttg caaaggcaac cgctaatgct
    14281 ccagtaccgc atgccagttc caaaatattt tttgtgtctt cttgtaaatg acgtttcgaa
    14341 aaagctaacc attgctgata aagtgtttcg tccatcactt catcgtaaat gaaagcaaat
    14401 gtttcataag ccatatgtta atccaaccat tcactaagat cgactagcgg agcatctgac
    14461 catagttttt ccagattata gaaatttcgt tctggcgcat ggaaaacatg tacgatcaca
    14521 tctccaagat cgatcaatac ccattttccg ccttcttttc cttcaatacg ttttacttca
    14581 tatttgtttt cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat ttgacgttcg
    14641 ctatttgctg aacagatcat gaaataatct gccaacaagg aaacatttcg aacgtctaaa
    14701 gcgataatgt cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt taattgttct
    14761 ttactatcta tgatgatttc ctcctaatat gagcagctta tttttttgct acccagtaat
    14821 tatatgtttc gattgttttt ggataaatct gttgttcttg cgcaagcaaa tgggccaatg
    14881 tatgttttgt ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg gctcgtgcat
    14941 cttgtacccc aggaaaatct cgacctggtt caatatagtc tgctacataa atgattttgt
    15001 caaggagact catcttagca gccccagtcg tatgcacccg gattgcatga agaatctctg
    15061 catcggtgat tcctaattcc cgctctacaa acgaagcgcc aacaagacca tgccagattg
    15121 cattattata attcaataac tccggatcaa aaccgtctct tacgattacc attttgaact
    15181 cctcatctgg tcgttctttt gcataatcat gtgtcaatgc agcaatacta gctttttcag
    15241 gagaagcacc gtattttttg gcaagagcca cggctgtttc ttctactcca agcacatgct
    15301 tgaagcgttg ttcgctcatt ctcatctgta ttttttgcat caggttttct ctagaataag
    15361 cagtatattc attactatag tccatattca tacaaccctt cattttgaat ataatcaatt
    15421 actttatctg gaacaaggta gcggatagaa cagccttctt tgatcttttg acgtattttc
    15481 gtggaactga tgtcgatctc aggtacatct acccaaatta caggatatgg agtatccgtt
    15541 gtgtatcctg gtcgacgtat acccacaaag ttgaccatag aagtcagctc atcgatttta
    15601 taccattttg gcaaatattc taccatatcc ccaccgataa taaagtaata atctgtatcc
    15661 ggattgtttt gcgtcaattc cttcatagta tcataggtgt aacttttccc accacgagct
    15721 aattcaatcg tttcaatctg caaaaaagga ttgtcttcca ccgctaattc caacatgttg
    15781 agtcgatgtt ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg ataagaaggc
    15841 atcaaaaata cgcggtcaag acctaaattc cggccagctt gttcagccat cactaaatgt
    15901 gcaaggtgaa cgggattgaa tgttccgccc aagatcccta cttgcttttt cttctctaag
    15961 aattgaggca tttcttcagg aaataattgt gaacgaacaa atgtcttagc ttgcgtattc
    16021 atcatggtca ctccttatat tgcttttact tcttttgata gcctttgata cttttctttt
    16081 gtggaaggtt tgtataatac aataacacgt ccaatgattt ggacgatttc gcaatctatt
    16141 tctttttcta atacttgagc tgcttcttct gcaatctcat ctgtattttg aagtaacgat
    16201 actttgatca gttctctttt ttctaaagct tcttcaattt gaacgatcat tgctgaattg
    16261 atcccacctt ttccgatttg aaaaatcggc tgaagatgat gcgctttgct acgtaaaaaa
    16321 cgcttttgtt ttcctcttaa actcataatt ttccttcttc cttaaatcaa tgctttgcgt
    16381 ttcaatacat ctacaccctt tggcgcccag cccgcaatta ctactggcgc tgttacagtg
    16441 atccagccta gaccggcaaa aacgatgtct gttttttctt tcacagaaaa ctcaaaacga
    16501 acaagctccg gaaatgcctc gacttcatca ggacgaggag gctgtaataa tccacctaca
    16561 tgtttttgat aaaattcgtc tgctttttcc agtttcgttc gatgaatatt tacatcattt
    16621 gacacataag caataaatga agcacgattg ccgctgataa aatcaaatct tgcaagtccg
    16681 cccaagaaaa gtgtttgccc ttcattcagc tgataaactt taggtttgat ttcttttaac
    16741 ggtgcaatga tccgcaaatc ttttttacct aagtagtgcg ccatttgatg gcgatggata
    16801 attcccggtg tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat tttatccaag
    16861 gtcgttcctg gaaactgcga agttgtgatt acatcttgta ctcccgctgt atttttgatg
    16921 atttgattga taagcgtcga ttttccgaca tttgttactc cgacgatata gacatcccgt
    16981 ccatcgcggt attcttcgat tttttctaat agttccgtca tctcatgaga tttcttagca
    17041 ctagttaata atacatctac tggacgaatt cctgcttcgt aagcacgttc cttcatccat
    17101 tgtgttaatt tagttttttt caatgattta ggtaatatat ccactttgtt cccaactaaa
    17161 agtactggat tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc attgaagtca
    17221 aaaatatcca ccacattgac gatcaatgca tctttttgcc ctaattcatt cagtaagcgc
    17281 aaaaagtcgt catccgtcaa tgagacatct tggatctcat tataatggcg taatctgaaa
    17341 caacgctgac aatagacttc cccggaagct aaaccttttt ccaaagccgt ttgcggcgtg
    17401 tatcctaatt caccgggttt gtttgtttgt ataacggcac cacagccgat acattgtaat
    17461 tcttctgtca ttcgattcca cctcgccagc ccatatcagg gtgttttgct aatagatagg
    17521 ccataattct tttttcccaa aaacgattaa attgtgtctt ccaactatct gttgcgataa
    17581 tgggttttac aaggatgcta cgtatccctg cacgattggc tccacgtata tcagtcataa
    17641 tctgatcgcc taccattact atctcatcat ctgctaaatc caattgtttc tttgctatgt
    17701 tgatccctct tgctaaaggt ttcattgctc gtgcaacata caaaagatca aatttttcaa
    17761 tcgctcgcgc gacacgtttt gaattattgt ttgaaacaac aacaacaggg attcctgcat
    17821 ttttcatttc taaaatccaa gtaagcaact cttctgttcc atctggatta ttccatgcaa
    17881 tcaacgtatt gtctaaatcg gtcaatactg ctttgatgcc tagattcttt aattgggcag
    17941 gtgtaatctg atagatggct tctaccatcc atgttggttt gtaatttgaa aacatgctgt
    18001 ctcctcatct gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg tattatactg
    18061 catatcagca aaaaaaacta cctctatact cgttcgtctt tcagaaaaag catttcttta
    18121 caaaatgacc aaaaataaag attgatcaca gaaaaattta gaaacaaaaa acaactaata
    18181 ttggcggaac ctcttgtatc gtttttccag taatttatgc tgatctaatc gaacaagttc
    18241 cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg actttatatc gtggcaactg
    18301 ctcgccgttg aacgtttcag gaataactcg atcaacgatt cctaactcgc gtagttctgg
    18361 cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat
    18421 cgacgcaaat ccttctggag aaagcacagc ataaatcgtt tcttccagca tccagacttc
    18481 atccgctaaa cctagagcaa gggcaccgcc acttccacct tcaccaatga tgatggcgat
    18541 gatcggtacc tttaaatcag acatttcgag cagatttctg gcaatagcct ctccttcgcc
    18601 gcgctcttct gctcctacac cgcaatatgc acctgctgta ttgatcaagg taatcaccgg
    18661 tctattgaac ttttccgctt gtttcatcag tcgtaaagct tttcgatagc cttcaggatg
    18721 gggagaacca aaatttctgc ggatattttc ctgcagatcc cgtccttttt gaataccgat
    18781 cactgttacc ggtttgccat ctaatcgagc gattccacca acaatcgctt cgtcatctgc
    18841 aaataaacga tcaccatggc attcctcgaa agaatcgaag atctcttcaa aaaaatccaa
    18901 gcttgtccat cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac
    18961 gatctatcca tcctttctgt gtatgcaatt caagaagcgt atggattttc tgtttcaatt
    19021 ccatgcgggg aacaatctga tcgacaaaac catgagataa taagaattca gccttttgga
    19081 aatcttctgg aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga
    19141 tcaagctttg gggttctgct agaataatat cgccttccat tgcaaaactg gcagttactc
    19201 ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag accttcattg ctatgccgtt
    19261 tgacagcagc agagattttt gccatctgca ttagagaaaa gattccttct tgcatccgtg
    19321 cgccacctga agctgtgaac aagacgactg gaagactttc ctttgttgct cgttcgaata
    19381 atctggtgat cttttctccg acaatcgttc ccatacttcc catgataaaa ttaggatcca
    19441 tcactccaat aatgaaggga atcccctgaa tggttgcttt accagtgagg actgcttcat
    19501 gaagtcctgt tttatcttgc atttttttaa ttttttccgt atattcggga aatgctagcg
    19561 gatctttggt tacaagatcc gtatcccatt cttcaaaact tttttcatca ataatcaaag
    19621 ctagacgctg ccatgcaccg attcgaaaag cgtatccgca atgagggcat actttttctt
    19681 caccgatgtc tttcgtataa aggatatgtt tgcaatttgg acactttgcc cacatgttat
    19741 caggaacaga tggcgagtta gcaggtttgc tctctctatt aggattgatc cgaatatatt
    19801 tctttttctt aaacagagcc atctatgcgc ctcctatctc cgtttctggt gtccaattcg
    19861 gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact gcaggatgac
    19921 taatcaaatc catctgaaat tctgcgttcg tgatgattcc ttctgtgata aattcactta
    19981 aggctcgctg catcttcatt aatgcttcaa aacgcgtatt tccatggacg attatttttg
    20041 caatcattga atcataatat ggcgggatcg tatatcccgg gtaaactgca ctgtctactc
    20101 gcagtcccat cccacctgct ggaagcagta agttttggat cttccctgga gaaggagcaa
    20161 aatggaaagc aggattttct gcattaatcc ggcattcgat tgcgtgtcct tggaatacta
    20221 cttcttcttg acacacattc aaaggctcac cggctgctat ctccacctgt ttttttacta
    20281 aatcgattcc tgtgaccatt tctgttactg gatgttccac ttggattcga gtattcattt
    20341 ccatgaaata aaaatcgcct tcttcatcca tcagaaactc gatcgttccg gcattttcat
    20401 aattgactgc tttagctgct cgaacagcag cgtctcctag tgcttctctt tttgtttgtg
    20461 agatcactac agaaggagat tcttccagta ctttttgatt gttgcgttgt aaagaacaat
    20521 ctcgctcgcc taaatgaatg acatgaccaa atcgatcacc taagatctga acttctatat
    20581 ggcgagcagg ataaatgatt ttttccaaat acatatcatc atttccaaat gccgctgctg
    20641 cttcttgctg agctgaagaa aaatgctgtg ggagctcttc ttttgattga actttccgaa
    20701 tgcctttccc gccaccacct gcagctgctt tcagcatcac tggataacca attctgtctg
    20761 ctatttccaa tgcttcttct actgtagata atacaccagt acttcctgga atgactggga
    20821 catttgcttc ttgcatcaat tgacgcgcat tgatcttatt tcccattgca tcgatcgttg
    20881 cacttttagg accaataaat gtgatattgc actcttcaca catggaggca aatcggctat
    20941 tttcagataa aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc
    21001 tcagtacttc ctgaacattc agatatgaat caacggcttt tgccgggcca atgcagattg
    21061 cttcatctgc taactgtgta tgaagtgctt cggcatctgc ttctgaatat atcgctacgg
    21121 tttggatccc tagttcgcgg catgcacgaa tgatacgtac agcgatttca ccgcggttag
    21181 ctatcaatac tttagaaaac atatttcacc tatccaatca taaaggttaa ttccgcttcg
    21241 gctacttttt taccgtctac tttagcgatt cctttgccta ttccggctga agctttcact
    21301 ttcaatatct caacttcaag gtataaggta tctcccggtg taactttctt acggaatttt
    21361 gctttgtcca atccgccgaa ataagccgtc tttcccttga attgttcaag agaaagtaat
    21421 gcaactgctc ctgcttgagc cattgcttca acaatcaata cccctggcat aacaggttct
    21481 tctggaaaat gtccttgaaa aaagggctca ttgatcgtta catttttctt agcaacgatc
    21541 cgttctcctt caaccatttc ttccactcga tcaatcaaaa gcattggata acgatgtgga
    21601 atgatttctt ttatttcttg tatgttcatt gttttccctc tttcctgctt tgcttatttt
    21661 tgctgaatcc taaagagtgg ctgtccatat tcgacaactg actcgttttc tacaaggatt
    21721 tctgtgatca ctccgtcggc ctcactagtt atctcattca tcagtttcat cgcttcaata
    21781 atgcagataa cttctccttt ggcaacttga tcacctactt ttttgaaagc tggtttgtcg
    21841 ggcgctggtt gcagataaac gattcccacg attggcgaaa caacctcgat tccatcaaat
    21901 actgccgatt ctttttcttt cattatttct ggagcagttg ttgcttgttc aggtatgaca
    21961 ttgactggct ccgaaactgg caacgtttcg tttgtttgaa ccatattcat gcctcgactt
    22021 gttttatttt tattcatata caattcaaat tgaccttcac gcaaatcgaa ttccgttagc
    22081 gaggattgat caaattgact gactaattct ctgatttcat tgatatccaa aattattcct
    22141 cccagcgttt catgcacaga accgcattat gtccgccaaa ccctaatgaa ttactcattg
    22201 cataagtaaa acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca atctcaggat
    22261 cttgtttttc tacattgatc gtaggtggga taaattgatg ttctaatgcc agcaaagttg
    22321 ccaaggcttc gatcccgccg gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc
    22381 tgacgtaagt atctctgaaa ttttcaccta atgcgatttg gattgctttt gcttctgcct
    22441 ggtcattggc tggtgtactt gttccgtgag cattgatata tccgacttgt tccggctgta
    22501 caccagcttc ttccatcgca agcttcattg ctttagatgc accagaacca tctggagtag
    22561 gagctgtcat atggtaagcg tcacagtttg caccgtatcc aacgatttct ccatagattt
    22621 tggcaccgcg agcttgtgca tgttctaaag attcaagaac tagaacgcct gctccttcac
    22681 ccatcacaaa accattccga ttttcgtcaa acggaatcga accacgatta ggatcagttg
    22741 attctgagag tgctgttaaa gctgcaaatc cagcaatccc catctcacaa atcgttgctt
    22801 ctgttcctcc agcaagaatg acatcagaat agccatgttt gatgttacgg aatgcttccc
    22861 caattgaatt cgttccgcta gcacaagctg ttacgatagc tgtacaaata ccttttgcac
    22921 ctacacgcaa agcaatattt ccagctgcca tgttggcaat cgccattggt acgaacattg
    22981 gagaaactcg ttttgcacct ttttcatgta gacgaataac ttggttttcg attgttggaa
    23041 gaccaccgat cccagatccg atgatgacgc caaaccggtc ttgattcatt ttttctttat
    23101 ctaaaccgct catctccatt gcttctaaag cagcgtagat tccatagatc gaaaacatat
    23161 ccattcgttt gctatctttt ttaataaaat atttatcgta agggaaatct ttgacttcac
    23221 ccgctaaggt aatccctgtt tctgaagcat caaatttagt gatcggtgcg atgccattcg
    23281 tcccctcttt taagctattc aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc
    23341 catagccagt aattaccact cgattcattt gtttgctctc cttttcttat ccgtgcatga
    23401 ccaatccgcc atcgacattg atgacttgtc cggtgatata tggattttgt gctaagaaaa
    23461 ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg catcggaatt tgcttttccg
    23521 cttgttcctt gactttgtct gctaaaactt ccgtcatatc agttgtgatg aatcctggcg
    23581 caatggcatt acatgtaatg cctctagttg cagcttctcg tgccacagat tttgttaatc
    23641 ctactacgcc agctttacta gctgcataat ttgcttgacc aatatttcca atcaacccag
    23701 atacactaga gagattaatg atggcccctt ctcgctgctt catcattttt ttcaatacgt
    23761 gctgcgtcat attgaaggtt ccaatcaaat tgatatccag acattttttg aaatcttctg
    23821 catccattcg catcacaagt ttgtcatttg tgattccggc attgtttact aacacatgga
    23881 tcgatccaag cttttcttct gcttctttga tcatttgtcc cgctttctca taatcagaga
    23941 tgtcaccgga aactcctacg cattttacac cgaatgcttc gatttcttct atcttttctt
    24001 ttggaatctc tccgcgacca ttcaaaatga tatttgctcc agcttttgca aaagctaagg
    24061 ccatcgcttt gccgattcct cttgtactac ctgtgataaa tacatttttt ccttttacat
    24121 ccataacgtt cctccttata gtattgctat tgtttcatca aatgtctgct tattctcaac
    24181 acgcagaact ggtattgttt tatcgatttt tttcataaat ccgcttaata cttttccggg
    24241 gccgacttcg atgacttgtt cgatgccgat tgtttttaac ttatggatac tctcatagaa
    24301 acgaacaggt tgcatgactt gctgttcaag tagtcccgcg attgtttctt tcttcattat
    24361 ttccgttgtt gtgttgctta tgataggaaa agaaggttca gagaattgaa tctgacttag
    24421 gtccttcgcc agtttttttg ctgccggttc taaaatagct gtatgaaaag gcccgctgac
    24481 atttagaggg atcattcttt tgaacccttt ttcttttaat agtaaaacag cttcgtctac
    24541 tgctttttct tcgccaccaa tcacgatctg ctgaggtgta ttataatttg caggagatac
    24601 gatgccgtac ttgctagctt catgacagct ttcctcgatc gtttcaatcg gcgcattcat
    24661 cactgcaacc attttcccgc ttccagctgg ggcagcttct gtcatataag cgcctctttt
    24721 tgctactaac gcaactgctt cagtgaagga tagcgcatta ctagctacta atgccgaata
    24781 ttctcctaaa ctcagtccta aagctgcatc tgggatgatt ccatgctcct tcaataagcg
    24841 ataatacgcg atgctgactg ttagaatagc tggctgtgta tattgcgtct gatccaaacg
    24901 ttcattttct gtaaagcaaa gttcagccat atcgtagccg agaatctcac ttgcttcatc
    24961 aaatgtctgc tttacaacgg cttcttcata taaatctttt cccatacctt gatattgtgc
    25021 accttggcca ctaaataaga atgctgtttt catgtttttc tccaatatat ccagaatata
    25081 tgtttgattc ttcctattca aaaaggccga gacattccta tttcaggaaa tgtcccagcc
    25141 cccaacctaa ttttaatgat tatgcagttt gtttttctac gtaagtcact aaatcttgaa
    25201 ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca atttcattga
    25261 tgatttggaa taaatccaag ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt
    25321 tgatttcttc agtttctttt cctaattctt ctgcgatgat ttcttgaatt ttttcgaata
    25381 ccataataat atattcctcc gatattgttt gctttttatt tttataattt aacagtctac
    25441 aactccatga gaatacttcc ccatgttaaa cctccgccat agcctgtaaa tattactttc
    25501 tgctgttttc ctaaggtgat ctttccacta gaaactgctt catccaataa gatagggatg
    25561 cttgcagcag atgtattccc atatttatcc atattggtca ggaacttttc ttgcggcatc
    25621 tttacttttc gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa aagcagataa
    25681 tcaactggtt tgtctttcat gacttcttgg atattgtttg caacgtctcg tacggcaaaa
    25741 tcaaaaatat ctctgccagt catttgcaaa taatacgaac cttctgaatc ttctgaatag
    25801 aacggatttt tattttgctg gtagccagcg gtcaatgagc tacttctggt accgtcagct
    25861 gctaattttt ctttcagcaa ttttttctgt ggattcgctt ctaagagaat cccacctgct
    25921 ccatcgccaa acaacacagc agtcgtacga tcagaccaat cgattagctt agatagtgtt
    25981 tcaccaccaa tcaccagtcc cttagttgag ccgcagcgga tcattttttc agccatgctt
    26041 aacgcataaa caaatcctga gcacgcagca ctgatatcaa aagcgatagc atgagttgct
    26101 cccaatttcc cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg cgacattgtt
    26161 gctactaaaa taaaatccaa atctttaggg tttagatttt tctgttttat caatttttcc
    26221 gccactaaaa tgcataaatc agaagtctcc tgatttgtta cacatcttcg ttcacgaata
    26281 cccgtccttg acgaaatcca ttcatcagag gtatccatca tcttggaaag atcattatta
    26341 gtaataatct tttcaggaag gacgctggct gttgcagtaa tcattccaaa ttgttccatt
    26401 aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag
    26461 gcatgctgtt cctccgtagc cattccgtct aagatgttct tgaccatttc acgatgaaaa
    26521 tgctgatgga cccgaaatag taactttcct tttttcgtta gtcccaactt tacaacgcgg
    26581 cggtcatctt cactgcggat acgttcgaca tagccttttt ttactaactt gttaatagca
    26641 gtggtcaagg tcccaacagt aattgatagt tctttggcta cttctgaagt tgtttttttc
    26701 ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga ttgacagatc tttgaattga
    26761 gattttttca gctcagactc ttcgattgtc aaaatatcat tgaagacact aacaagataa
    26821 tcatttactg tttttaaatc tggttccatg ttccaccgtc cactaattag tttgattatc
    26881 aaatcttttg atgttcaaac tatatcttaa cttttttcaa aatgcaacct taaaagatca
    26941 tttttttact atatgagctt tttttaagat tttactcat
//
LOCUS       DAJSMI010000019        25601 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJSMI010000019 DAJSMI010000000
VERSION     DAJSMI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.019"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            135..794
                     /locus_tag="ON430_000686"
     CDS             135..794
                     /locus_tag="ON430_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3218629.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            879..1784
                     /locus_tag="ON430_000687"
     CDS             879..1784
                     /locus_tag="ON430_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3218630.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1786..2418)
                     /locus_tag="ON430_000688"
     CDS             complement(1786..2418)
                     /locus_tag="ON430_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR3218631.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    2569..2694
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2738..3262
                     /gene="infC"
                     /locus_tag="ON430_000689"
     CDS             2738..3262
                     /gene="infC"
                     /locus_tag="ON430_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR3218632.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3334..3534
                     /gene="rpmI"
                     /locus_tag="ON430_000690"
     CDS             3334..3534
                     /gene="rpmI"
                     /locus_tag="ON430_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR3218633.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3587..3946
                     /gene="rplT"
                     /locus_tag="ON430_000691"
     CDS             3587..3946
                     /gene="rplT"
                     /locus_tag="ON430_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR3218634.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            4198..5535
                     /locus_tag="ON430_000692"
     CDS             4198..5535
                     /locus_tag="ON430_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCR3218635.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            5583..7712
                     /locus_tag="ON430_000693"
     CDS             5583..7712
                     /locus_tag="ON430_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCR3218636.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFTKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            7687..8376
                     /locus_tag="ON430_000694"
     CDS             7687..8376
                     /locus_tag="ON430_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218637.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(8784..9470)
                     /locus_tag="ON430_000695"
     CDS             complement(8784..9470)
                     /locus_tag="ON430_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3218638.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(9606..9800)
                     /locus_tag="ON430_000696"
     CDS             complement(9606..9800)
                     /locus_tag="ON430_000696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218639.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(9850..10290)
                     /locus_tag="ON430_000697"
     CDS             complement(9850..10290)
                     /locus_tag="ON430_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCR3218640.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     SYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(10294..11139)
                     /locus_tag="ON430_000698"
     CDS             complement(10294..11139)
                     /locus_tag="ON430_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCR3218641.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11288..12706
                     /gene="pepV"
                     /locus_tag="ON430_000699"
     CDS             11288..12706
                     /gene="pepV"
                     /locus_tag="ON430_000699"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCR3218642.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(12763..13710)
                     /locus_tag="ON430_000700"
     CDS             complement(12763..13710)
                     /locus_tag="ON430_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3218643.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(13933..14283)
                     /locus_tag="ON430_000701"
     CDS             complement(13933..14283)
                     /locus_tag="ON430_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR3218644.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            14483..15562
                     /gene="pepA"
                     /locus_tag="ON430_000702"
     CDS             14483..15562
                     /gene="pepA"
                     /locus_tag="ON430_000702"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCR3218645.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            15706..16026
                     /locus_tag="ON430_000703"
     CDS             15706..16026
                     /locus_tag="ON430_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3218646.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            16048..16512
                     /locus_tag="ON430_000704"
     CDS             16048..16512
                     /locus_tag="ON430_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3218647.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            16717..17322
                     /locus_tag="ON430_000705"
     CDS             16717..17322
                     /locus_tag="ON430_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCR3218648.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(17361..18650)
                     /locus_tag="ON430_000706"
     CDS             complement(17361..18650)
                     /locus_tag="ON430_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCR3218649.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            19078..19557
                     /gene="rlmH"
                     /locus_tag="ON430_000707"
     CDS             19078..19557
                     /gene="rlmH"
                     /locus_tag="ON430_000707"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR3218650.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            19642..20151
                     /locus_tag="ON430_000708"
     CDS             19642..20151
                     /locus_tag="ON430_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR3218651.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            20251..20901
                     /locus_tag="ON430_000709"
     CDS             20251..20901
                     /locus_tag="ON430_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218652.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELWGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            21352..22290
                     /locus_tag="ON430_000710"
     CDS             21352..22290
                     /locus_tag="ON430_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3218653.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            22303..23355
                     /locus_tag="ON430_000711"
     CDS             22303..23355
                     /locus_tag="ON430_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3218654.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            23621..24256
                     /locus_tag="ON430_000712"
     CDS             23621..24256
                     /locus_tag="ON430_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR3218655.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            24447..25238
                     /gene="msrA"
                     /locus_tag="ON430_000713"
     CDS             24447..25238
                     /gene="msrA"
                     /locus_tag="ON430_000713"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3218656.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         8168 a         4490 c         5287 g         7656 t
ORIGIN      
        1 tttcttaaga attttttgac tttttttctt ctttgactag aaaaaaatac aattgacact
       61 actagatatt gggggtatat ttaaaaaggt tggagttaaa cacaatattt agtgatgaag
      121 tgagctggag gtaaatgagc gtgatcgttt tagcaggtac aattggtgcc ggaaaatcaa
      181 gtttaacaga aatgatggct gaacactttg atagtcaggc attttatgaa tcgattgatg
      241 ataatgaagt cttgccctta ttttatgcaa accctgaaca gtatgctttt ttgctgcaaa
      301 tttatttttt gaataaacgt tttgcaagca tcaaacaagc aatgaaagat gacaataatg
      361 tattggaccg ttcgatttat gaagattcac tgcttttcca tctaaatgca gatctaggaa
      421 gagcaacaga aacagaagtt cgtgtgtatg atgaactgct ggaaaacatg atggaagaac
      481 ttccctatgc tgcccataaa aaacatccgg atttattagt tcatattcgt gtatcttttg
      541 atacgatgtt ggagcgtatc gaaaaacgtg gtcgttcgta tgaacaattg tcctttgatc
      601 caagtctata cgattattac aaagaattga acagacgtta cgatcaatgg tatgaagaat
      661 acaaagaaag tccaaaaatc caaatcgatg gagaccgcta taattttgtg gaagatcctc
      721 aagcaaaaga agaagtcttg aaaatgatcg aagaaaaatt agcagagatt cgccaaacca
      781 aagtagccat ttgatttaca gttgctcaaa taaagggtaa acctttattt gagcgacttt
      841 tttctttata ctggaaacaa atgtcaggag gatagagtat gcgtgaaaaa cactatttta
      901 atcaagaccg taatttttgg atcaatgtac agcaaaacga tgtgctagct gttaaaaact
      961 taaaagaaga atatggcatt agtgacgaga tgctgactta ttctctagat aaaaatgaac
     1021 gggcgcgcgt agagtacgat tcttttgatg aagcgttact gcttgtatca aatgttccgc
     1081 accagcaaaa agtcgataat cattatgaaa ctagtcctat cgcttttatt ttaaaagaag
     1141 acggtttgtt tacatttacc acgccgaata ccgagtacgt gattcgacta attcgatcgc
     1201 ttttggaacg aatgccagac atgagtgtat acagtttatt gtttcgtaca ttgtttttga
     1261 tttctgattc cttttttcct ttaatcgagg aagtgaatag tgaacgtcag cggcttaatc
     1321 tgaaattaag agaaaaaaca acgaacaaga atctgctgca actttcagat ttagagatcg
     1381 gtttggttta cttagttaca ggaacaaaac aaaatgtagt acttttagaa caaatcaagg
     1441 cattggctat ttatcgcaag ttgtcagaaa aggaaaaaga agagttggac gatgcgctga
     1501 tcgaagcacg acaagcagtt gaaatgacga acttagcttc gcagatttta gaccagctct
     1561 ctggcactta taataatttg ctgaataaca acttgaatga cacgatgaaa tttttgacgg
     1621 tctggtcgct gatcttgacc gttccaacta ttgtgacagg attcttcgga atgaatctcc
     1681 agttaccctt tacccattcc gttttcggat ggggaatcgc cttgatcatc agtttggtct
     1741 tatctatttg gatgcttatt gcactgtggc ggcggattcg ttgatctagt ctttgatctg
     1801 caaaccaagg gcttgggcga tcacaagtgc ttgctcttgg ttgacgatta gtcccgccaa
     1861 agaatcttga gataaagcga ttgctgtgaa gaaattagtc gtaaagtcta atcccttcag
     1921 agaagttcga aaccagttcg aaccggtcaa ctgattgtca gacaaaaaaa ggtttttcca
     1981 agtgacctca taaaattcgc tgtcttctaa ttgacaatga tcgaaggaga cagctttcaa
     2041 gtttgtacta ctaaaggaag ccatcgaagc aatacaatca gtgaaagtac agtctcgaag
     2101 ataactttcg gcaaaattcg tacctgttaa tttacattga tgaaacacga cttggtggaa
     2161 actgccacca atccactcca gattggaaaa atcacatttt tcaaaaatga cattactgca
     2221 ttcaaaatga cgtaaaactg ttttttgcat cgtaagtttt tggatatgac tctctcttaa
     2281 taccatattt ttggcatcga gatagctttg gtcacccccg ttgatcagaa gaccagagaa
     2341 gatctcctca tcttctaaat aaaactctcc ctttgtaaga agaggaagag aaggtggaat
     2401 aggttcgatt tttttcatgt gggttcacct cgtttttctt tagtatagca aagaagcggg
     2461 aatcactgct agaaagtctg ctaatctaat cattttcaga aaaggaagac aaactgtatg
     2521 aaaaacattg acaaaaagat tagtttttgc tatactatga tggttgagaa ataaaagcag
     2581 aagcacccgc ttctcgcctt agttgataat gtcactgggc tagatagttt tcactggttc
     2641 tattaggtga agtacgcgcg ggttctgtaa gagaactcgt ttttttattg ccgtttattc
     2701 ggtaagcttt tctttctctc ataatatttg gaggtgaatg accatagcaa aggatatgat
     2761 ggttaacgac ggcattcgtg cacgagagtt acgattgatc ggttcagatg gtgagcaatt
     2821 aggagttaag acaaaagcag aagcattgaa tattgcagaa caagcaaact tggatcttgt
     2881 gttagttgcc cctggtgcga aaccaccagt tgcgcgaatc atggactacg gtaaataccg
     2941 tttcgaacaa caaaagaaag accgcgaagc gcgtaaaaaa caaaaagtga tcaatgtaaa
     3001 agaagttcgc ttaagtccta cgatcgactt gaatgacttc aatacaaaac ttcgtaatgc
     3061 acgtaagttc ttggaaaagg gcgataaagt gaaagcttct atccgtttca aaggccgtgc
     3121 cattacccat aaagaaattg gtcaaaaagt cttgaatcgc ttagctgaag aaactgcgga
     3181 tattgcaaca gtggaacaaa aagcgaaaat ggacggacgc agcatgttct tgacgctggc
     3241 accgaaaaac gacagtaagt aatttgaatg actaactggc tgttggacag ccgatagtcg
     3301 gataacgaaa gattttgagg aggaaatata gtcatgccaa aacaaaaaac acaccgcgga
     3361 tcagcaaaac gtttcaaacg tactggtaaa ggcggattga aacgcttccg cgcgtttaca
     3421 agtcaccgtt tccacggcaa aacaaaaaaa caacgccgtc aattgcgtaa agcaagcatg
     3481 gtttctagtg gcgacttcaa acgtattcgc caacaactag caaaaatgaa ataagaagta
     3541 caaataacag aatctgaaga tttattttta ttaggaggaa ttatacatgg cacgtgttaa
     3601 aggtggaaca gtaactcgta aacgtcgtaa aagagtgcta aaattagcaa aaggttacta
     3661 cggttcaaaa cacactttat ttaaaacagc aaaagaacaa gtaatgaatt catacaacta
     3721 cgcatatcgc gatcgtcgtc aaaagaaacg cgacttccgt aaattgtgga ttgctcgtat
     3781 caacgcagca gctcgtatga atggcttgag ctactcaaaa ttaatgcacg gtttgaaagt
     3841 tgctgaaatc gacatcaacc gtaaaatgtt agctgacttg gctgttaacg atgcagcagc
     3901 atttactgca ttggctgacc aagcaaaaga agctttggct aaataagcta aacaattata
     3961 aagaggaaag aagataacga aaagttatgt tctttcctct ttttttattt acaatcctcc
     4021 atcaaatgaa acaggacaat aacgtataag tctaaaatag cataattaag gttttttaat
     4081 tgaaaactga acatttatgg tcacccttta atgaaagcgc taactacata agttagaatg
     4141 agtttgactg atatattagt ttgcttatca gaaaaacaaa atggacggag gaataacatg
     4201 agtgatatta ttattggtat tttgttagtt atctcattct ttttcatggt ctggtactgt
     4261 gtaaaaggct acaacttgat ggtcggcttt gccatcatgg ctactgtatg gatgggatta
     4321 gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg aagggcaagg agtaattgat
     4381 gtgttgacgc atatctatac gacggggcca gctgagtacg ccaaatcgat cttggtaaat
     4441 gtcttctttg gtgccttttt cgggcgtgtt ctagtagata gcggaatcgc agctaccctg
     4501 attcgaaagg tagttgaact tggaggggac aagccaagaa tcacgatgag cttactatgt
     4561 attgtaaccg ctgttatatt catgtcgatg acaggaatcg gaccagtgat ttctattgca
     4621 gttattgtgt tgccaatctt gatgtcatta ggtatttcag taccggttgc actgttctca
     4681 tttatgggct cgattatggc tggaatcttt gctaatatcg tcaatttcaa acaatatcaa
     4741 acgatttatg ctggtttcaa tccagcggct gaaagctata cttataatga ctatttccaa
     4801 attggtatga ttgggatgat tgtaagctta gttgtggtac taacggtcgc taatatatca
     4861 atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac cagctgaagg tggcgacgca
     4921 ccaatgatct catggttagc cgtattatta ccagttttag gggtcgttct tttagatctt
     4981 ccaatcatct taggttttat tctagcaggt atctgggcat tgctgtttac tggaaaattg
     5041 cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat tatttacaga tggcgcagta
     5101 gatgttgcac caatggttgg tttcttgatg accttagcga tgttcaataa ttcagctgcg
     5161 tatgcttctc cttatttttc agctattttt ggagattgga taccgcaatc accattagtc
     5221 ttggcaatcg tatttgctat attgacacca ctaggatttt tccgcggtcc aatgaatcta
     5281 gtcggttcag gctcagctat tttagctgtc gtattagctg ttaatccaac gatgtcgcca
     5341 gcctttttgt tcccgttatt tgcaatcaca acgattgctc ctcagcattt agatattaca
     5401 caatcttggg ttgcttgggg attaggttat acaaaagtaa cttctcgaga atatatgaag
     5461 aaatccatcc caactggttg gatcatcgga gcgatcttat gcctgatcac gttcttattg
     5521 tacgggaacg cctagtaaac aaaacgtcga taaaaacgat tagatgaatg gaggaaaaga
     5581 aaatgaaacg ttttgtgcga atgggaattg atgttggcgg tacacatacg aaagcagtcg
     5641 caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc agtgaaaaca acccacgatg
     5701 atgtccgtgg agtagccgca ggtgtcgttc agtcttttca aaattgtttg cgggaaaata
     5761 atatcagtcc agaagatgtt gtgtttgttg cacattctac cacacaagcg actaatgcac
     5821 tgattgaagg agacgtagca aaagtcggcg taatcgggat ggcaaaaggt ggattagaag
     5881 gttttttagc aaaacgacaa actagattga acgatatcga tttagggaat aagaaaaaaa
     5941 tagagattgt caatgcattc ttacctgtca aacacttaaa tgtagaccgt gtttcagaaa
     6001 cgatttcttc tttagaaaga gaaagagcgg aggtcttggt ttcttctatg gcatttggag
     6061 tagataatgg ggaaccagaa cgagttgttt atgaagcagc atcggtaaaa tcgatcccta
     6121 caacgatggc ttctgacatc acaaaattat atggattgac aagaagaact cggacagcag
     6181 caatcaatgc atctatttta ccaaaaatgc tagatacggc aacctctaca gaagattccg
     6241 tacgagaagc aggggtcaat gtttctttga tgattatgcg cggagacggc ggagtaatgg
     6301 aaatcaatga aatgaaaaaa cgacctgttt taaccatgct ttccggtcca gcagcatcag
     6361 tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt ttattttgaa gtaggcggaa
     6421 caacaaccaa tatcggggtc attaaaaatg gacgtccagc aatcgattat tcaattgtag
     6481 gtggccaccc aacatatatc tcttcattag atgttcgggt tctaggtgtg gcaggcggtt
     6541 cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg tccacgttct gcacatattg
     6601 ccggtctaga ttatgcagta tttacggaga cagaaaaaat caaaggtcct aaggttgaat
     6661 ttttctctcc taaagaagga gaccctgctg attacgtcaa ggtagtgatg gaagacggag
     6721 aagaagtgac tattacaaat acatgtgcag ccaatgtcct tggattagtg caagaagaac
     6781 atttttctta tggaaatgtc ccatctgcac gaaaagccat ccaagcatta gctgattatt
     6841 gtcatacgac ggttgaagac atcgcagaac agatcatgga aaaatcctat gctaagattg
     6901 aaccggtgat tttagaattg gcagacaagt atcacttgga gaaagatcag atttctcttg
     6961 taggtgtagg gggcggcgct gcctcattga ttacttactt tagtaataaa atgggtgtga
     7021 aatattcgat tcctgaaaac gccgaagtga tttcttctat tggtgtagca cttgcaatgg
     7081 tccgagatgt tgtagaacgt atcatccctt cgccaagtaa agaagatata cgatcgttga
     7141 aaaatgaagc gatgaataaa gcgattgaat caggcgctac accagaatcg atcgaagtcc
     7201 acgtagaaat cgatccacag acttcaaaag taacggctat cgctactggt tctacggaag
     7261 tgaaagcaac cgatctaacc aaggagatca caacagaaga agcactagag cttgctgcag
     7321 aggatatgcg attaaataaa aatgaagttt gcttattgga aaatacacct ttcttttatg
     7381 tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct tcggatcatc gatcaaaaag
     7441 gatttaccaa ggtccaacgt ggacatgctt catgtatgaa aacaactgca gccaattata
     7501 tgacagctgt cgaacaactt tgggaagata tggcagttta tcaaactgaa ttgattgccc
     7561 gaccagaatt ttatctctgc ttaggagcaa gagtctctga ttttacagcg acggatctgg
     7621 aacaactaca gcttttgatg gatctggaag tatctactat ggaaccggag gaagaagtga
     7681 tcgtcgttgc cggaaatatc aaacaaacat aagttagaag agttactaaa cgaattacaa
     7741 cagatccccg aagaaatttg gggattctac cagtttcggc gagatttatt ttggaaaaag
     7801 attcctttgt ccaagcagaa aattcttata caacaatcaa tcgattgtgg tatcgaaacg
     7861 gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag gagaaatttg tgaacaaatg
     7921 gctgttccaa tcgtttcctg tgaatcagag caaataaacg aacgaatcac ttttgcaact
     7981 tatgcagaag atgaagggat tcgattgatg acggagccgt tagaaaaatt aaaatgttca
     8041 ggactagctt cgatctctaa agaaacagcg caagctttga ttattggaca tgaactattt
     8101 caccacatcg aagcttctgt taagggaatt tatacgcaaa gcgaaaaaat tgtgctgtgg
     8161 cggttgcctt tttatacaca tcaatcaaac attcgtgcat tgagcgagat agcagcgatg
     8221 agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt atgtattaga ggcagtctta
     8281 ctttggccat ataatgaaat tcgtagccaa gggatcttag aagaaataaa aaaaatagaa
     8341 aaaagatgta cggagtatga tttagattac aaataaagag actgtgacaa aagtcgtgtc
     8401 acagtccttt ttttccgaat aaacgatgtt ctcctgtcaa ctcctcggtc acaagtcaaa
     8461 atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc ggagatgaac
     8521 ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct tgcatctgcg gtaataagtc
     8581 cagccaagtt aaaaacataa gaagctattt ttacttggct gttcttatta cttgatgcag
     8641 ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc agctgaccct cggtattagc
     8701 tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt tctttctcta atactcaggt
     8761 caaaacgctg attccaaaac ctcttatttg atgggtctgt cctgaatatc ttcaaaataa
     8821 tcggggtaat tatgaatcac gtattcttgc tcctcgataa tcatatgtaa atgcatcgtg
     8881 caaagaaatc cgacttcatc aaagttatga tcaatcattg attgaagcat tgtctgatgt
     8941 tcatctaata ctcgacccca gtctaattct gaaatcgtca aacgtaacca tctgaaacgg
     9001 ttcaagtgcg tactataact ttctagccaa tcccaaattt ctcgttttcc agcaatttca
     9061 taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag cttttttatt ttgagcgatt
     9121 actgcctgat cggtttgttc tagtattttt tctagtttag cttgatcttg cttggtcatt
     9181 ttcgcactgc attcttgcag gatcggtttt tccaactttt cccgcgtata acgggctaat
     9241 gaagcagatc ggatatcgat ttttgaaaca taagtgccgg actgtggaat ggtttttaca
     9301 agctcttgat tgcgcagttg gatcagtgct tctcgaatcg gtgtacgccc gatagctagt
     9361 tgttcttcta acactttatc ggagatttta cttccaggtg ctaactcgga ataaatgatt
     9421 tgtttacgta tagattcata agcttgctgt tgcaaattat tgataaccat ttaaccttcc
     9481 tttctatgca gatataatct attataacat aaatcgctta caaatcgtgt gggcataaaa
     9541 acacagtctg caaaaaaagc cctgataggc atgcctatta gagcctcgaa aagaaaaata
     9601 ttgaatcaat ctggtcgtac ctcttgtatc ggtttgacac gaccgccgat tcttttagca
     9661 aaagcttcgg attctttttt gtctttgaaa atgagcagat tgtctaagtt tttttcccga
     9721 gtgcgacagc cagttttatc gatgtaaccg tgcattactt taacgacata catggggatc
     9781 acctgctttt ttcttttcat tatacgaact actcgacaca atacaagaaa aatgcttaga
     9841 cggtctttat taagaattta catcatccaa agaagcgtac gcttggaata actgatctaa
     9901 ctccggattg actttgctca gcttcactaa tcgttgcgaa tcagaaagta aaaaacagtg
     9961 tttactactg ccagtagttt ttaatttttt gtcttcagtt cccacaattt cgtagctaaa
    10021 atccaaccgt gtccccgtat acttttggat agttggatgg atgctcactt tttctccgta
    10081 atggatcatt tctttatagc tgcaatttac ttccagaacg gggatgatga tcccagattc
    10141 ttcgattttt ttataggaaa accctagatg taccaataag gctacccgag cctcttcaaa
    10201 ccagcggata taattgctgt gatgaataat ccccatttga tctgtttcat aataaaaagg
    10261 ttctcgcaca taacctgtaa atttctccat gactcatgcc tcgttttcca ttgatttttt
    10321 acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt tgtgtcggca aaacaatata
    10381 ttgttctttt gccagctggt ctccaatgaa actatgaaga taaatagctg ctgcaatcgt
    10441 ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc atcccagcta aagtgtcacc
    10501 catgccgccc gttgccatag cagcagaacc taaaggattg gtgtagaagt gctggtgacc
    10561 ataaattttt gtctcatgac ttttcaaaac gagcacagca ccgatttcct gttgcttctt
    10621 tttgttttct ttatcccgtt gttcttgtat cggtagagaa ctcaaccgtt gccactccat
    10681 ttgatgaggt gtaagtacag tttgttcggg atagggtagc aaaagatgat tagcagcaag
    10741 taaacttaaa gcagagccat caatcaccag ccattgttct tttgattggt tttctagtac
    10801 ttgtttgaaa cgttccaaac tcttagaaga agtacccaat ccgggaccaa tcaagagtac
    10861 atctgcttgc tttattacgg aaagaaactg gtcagtatcc gtccaaacta caatcattgc
    10921 ttctggcaga tgactgtgaa gagcactccg atttttttca tcagttacta cagtcgtgag
    10981 acccgctccg ctgtgtacgg cagctaaagc actcataatg attgctccgc catattgttc
    11041 attgccgcca atcaaaacga tacgtccaaa tgttccttta tgtgaaattt ttggacgagg
    11101 tgtgatgact cttttcacta gttgttcgtt tatcagcatc taaaaacctc cttttactca
    11161 ttatagcgta tatttttttt agagaaaaac agataagaaa aggaattgtc agattttttg
    11221 aactattttt ttctgtaatt tgtggtatca tgaacacgaa gacttaaata agtggaggat
    11281 taactttatg acaattgatt ggcaaaaaga agtagaagca cgtaaagaag acttgcttga
    11341 agacttgaaa aatcttttac gtgtcaacag cgagcgcgat gactcaaaag taacaccaga
    11401 tgcaccattt ggacctggac ctcgcgacgc attgaaacat atgttagctt atggcgaacg
    11461 tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg catattgatc ttggcgaagg
    11521 agacgaaacg ctagggatat ttggacacat ggatgtcgtt cctgcaggag atggctggga
    11581 cacagatcct tatgaaccag taatcaagga tggaaagatc tttgcacgcg gatcaagcga
    11641 tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag atcattaaag aacttgactt
    11701 gaaactatca aaaaaagttc gcttcgtagt cggcagcgat gaagaaagcg gctggggcga
    11761 tatggcttat tatttcgaac atgaagaaga accagatttt ggtttctcac cagatgctga
    11821 attcccaatc atcaatggtg aaaaaggaaa tgtttcttta gctttacgct tcaaaggcga
    11881 caatgcagga gattatgtct tgaaatcttt tgtttccgga cttcgcgaaa atatggtgcc
    11941 aggaacagca actgcagcac ttcaagtacc aagtgcagat gcagctatcg cgatggaaga
    12001 agcattttac caattcatcg aagcaaatcc agtaagcgga acgattgaag cggacaatac
    12061 gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt gcaagcccac aatctggtat
    12121 caatgcaggt tctttcttgg cattgttctt agataactat gaattcctag gatctgctaa
    12181 acaatttatc catgtagcag ctgcttatgt ccatgaagat ttttatggag aaaaactagg
    12241 agtcgcttat gaagatgaaa aaatgggcaa attgacaatg aatgctggac tatttgcttt
    12301 tgaagaaaat ggtacagagg aagcgaactt catcaatatg aatttccgtt ttccaaaagg
    12361 tgtaacagta gacggattgc aaagtgacat cgaacaaaca gtggggcaag aaggcgcaac
    12421 agttactcga ggtgctcgcg taatggaacc acattacgta ccaatggatg atccattggt
    12481 ggctacatta ttgcaagtct atgaagacca cactggagaa aaaggctacg aacaaatcat
    12541 cggtggcgga acttacggac gtttgttgaa acgtggcgtt gcttatggag cgatgttccc
    12601 aggatatact gatacaatgc atcaagcaaa cgaatttatg agtcttgatg acttgttcag
    12661 agcaacagca atctatgcag atgccatcta tcgtttggcg aaataattaa ttaaaaagag
    12721 gatcaagaat tgcaaattat ttttgcagtc ttgatccttt ttttactttc tactgtggta
    12781 ttcatgataa acatgatata gattcaattc atattctttc ttagcgttcg tgacgactgt
    12841 atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa gtcatcaatc cagtacttaa
    12901 aatcgcagaa gggatgcgtg taataaagct tgtcagcata tattcgcgaa tcacaggaat
    12961 ccccgtgacc aatccaaata aaaagaagaa gatggtccaa atcccaaaga aaagcaaagg
    13021 cttataatct ttaaacattt taataatcat catgatcact ttgaatccgt cagagaatgt
    13081 attcaatttt gattcgctgc cttcaggacg atcacgataa tcgattggga tttcaacgaa
    13141 cttgaatttt ttatctaaag catggatcgt caattccgtt tcgatttgaa aaccagaact
    13201 cataatagga aaacttttga caaagatacg attgaatcct cgatagccgg tcatgacatc
    13261 aagaattttt gttttataca ttctagtaat tgtatttcta acaaggttat ttccaaaatc
    13321 gtggaaaggt ctcttattct catcaaagta ggttccattt gataggcgat caccaatgac
    13381 catatctgct tcaccagaac gtaatttctc gagcaaacca tgtacagctt ctgcaggata
    13441 agtatcatcg ccatctacca ttaaataata gtcggcatcg atatcaaaga acatttggcg
    13501 gatcacattt ccttttcctt gacgaggttc tttttttacg attgctccac cttgaacggc
    13561 caattcgtat gttcgatcgg tcgaattatt atcatagaca taaatatctg cttctggtaa
    13621 ttctcgttta aagtctgcaa tgactgttga aattgttgct tcctcattgt aacaaggaat
    13681 cagaacagca attttttctt gttgttccat ttttcactct ccttaactat agaaaacaaa
    13741 agctaaatta aaaatacctc ataatcaaag gaaatgctag tttttcccgc taaatatcat
    13801 ttatttaaaa agaaaatact ggaatatatg tttggaaagc tctatctaaa aaaccaccca
    13861 ctacaaaaga aggacatgat acctctcttt tgaatagtgg gtgcgtattc gttctctcac
    13921 ttttttgacg tattaatctg ttgcaccttt ttttcttgaa atttctaata cggttcctgt
    13981 atgagcatct gctaaaaatt cataataaac agtttcgcca tcttctattc gagaaagacc
    14041 tccccgataa gcactcgacc gaacggcgaa tttcctaata ggttcctttt caaaagagat
    14101 ccaagaacct tcgattgcac cttcttttag aaaagatgac ttgatatttt caagtacttg
    14161 gtctgctgac aatacttttt tgcgatgata ccaaatcgtt gaagctaccc cgctcaataa
    14221 tcctagaccg attccaactg ctaacccgcc tttaaaatag cttagttcgc tttcttcata
    14281 catgtggaca tacctccttc ttctcacttg ttactccttg tgcttatttt accacactct
    14341 catattttct ttgaaataaa taaccgctac cagaaaaaat aagctataat aaatacgatc
    14401 ggtctgcaga aaaaatcata ttttatccgc agatcaatat aagtctgaga ggagtttagt
    14461 ctgaaattta gacgaatagc atatggaaga aaaaacattt caacgaatca aagagttaac
    14521 agaattacaa ggaacaagcg gttttgaaca agatgttcgc gcttatatgg aaagtcatat
    14581 ggaaccgctg gtagatgagt tacagttaga tggtttgggc ggcatttttg gtctgcgcca
    14641 tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca catatggatg aagtcggatt
    14701 catgctgaca caaatccaag aaaacgggct ttttaaagtc gtacctctag gcggctggaa
    14761 tccttacgtt gtttctgctc agcgttttac acttaaaacc tctactggta agaattatcc
    14821 atgtatttct tcttccgttc ccccacattt attgcgagga acaagcggac aaaaatcagt
    14881 agaagtaacc gatattttgt tcgatgctgg gtttgaatca agagaagagg caatgagtta
    14941 cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa acgatcaaaa cagccaacgg
    15001 aaagaatatt atcagtaaat catgggacaa ccgctatggc tgtacaatgg tattagaagc
    15061 tttagaagcg ttgcaaaatg aaacattagg tcatacattg attgcaggcg cgaatgtcca
    15121 ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat aaattcaagc cagatctgtt
    15181 ctttgcagta gactgctctg cagcagatga tacagtgacg aaaaatggca cattcggaca
    15241 tttaggagaa ggtacattga tgcgtatcca agatccaggt ttgatcatgc ttcctcgttt
    15301 gcgcgagtac ttgctggata tcgctgaaac aaacaatatc ccttatcaat actttgtttc
    15361 aaaaggcggt acagatgcag gtgctgctca tacacaaaac gaaggaatcc caagtacagt
    15421 gattggcgta gtgggacgtt atatccatac gcatcaaacg atgttcagta ttcgcgattt
    15481 tgaagctgca cgagaaatgt tgattcaaac actgaaaggt ttggataaat caacagtgaa
    15541 tacgatcgta tacggaaaat aatgcgatag tgaaactata aaatctggca aaagattgct
    15601 ttttgccaga ttttttttat aaaaagagga tcaatgtttg ttagttcgaa agaaaggtgt
    15661 ataatttaat tgaaaaatac ataaacagaa agtagaggac aaagaatgat tatcccaaat
    15721 agtttagaag aattagcagg ctacgtagaa actggaaaga gtgtattttt ctttactgcc
    15781 ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc ctgaaattga aaatgacttt
    15841 tctgattggc aatttattga agtagaccgt gacaagtaca tagatgtagc cgctgaatgg
    15901 aacatttttg gtatcccaag ttttgtcgtg atccaagacg gaaaagaatt aggtcgtcta
    15961 gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt tcctagaaag tgtaacaaaa
    16021 ggctgacatc tgttaggagg ggtcaagatg aatcaagaat ataagaatat tttagtaggt
    16081 atcgacggta gtgaacaagc acttgctgct ttcaaaaaag cggtagaagt ggctcgccgt
    16141 aataatggga aagtatatgt agcaaacgta atcgatcagc aattttataa ctttatgggc
    16201 tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg aaacagccaa ggaaatgatc
    16261 gaagaatgta aagaatatgg gaaatctgtt gattacacaa atatagaagg aattattgca
    16321 tatggttctg ttcgtgaatc catggcagtc aaattgccaa aaaaatatga gatcgatcta
    16381 atcatggttg ggcagtcagg tctaaatgca gtcgaacgct ttatgacagg aagtgtagcc
    16441 agctacgtga tacgtcattc accttgtgat gtcttgatcg tttctgacga agaagaacag
    16501 ccaagtaatt aagcaagtgg aacttgtacg acctttttaa tcaattaact aaaacagtaa
    16561 agggactgtg acaaaagtct tgtcacagtc ccttgttacg aataaatggt gttccagaag
    16621 aggcttcttc ggaaataaag catgaaaaaa atttcatgca gtgaatgcaa ttttttgtta
    16681 agataaatct cagcaaagga taatggagga aagaacatga tttttgctta taacaaagca
    16741 cacgtcggag acaccttgat ggttattata gccgacgata aaggaacaga aaacactgtt
    16801 gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag gtcaagttgt tgcttggaac
    16861 tttttccata tctccgatca tctaacaatc gaagggaacg gccaagtaag cttaacagat
    16921 gaggaattag cgaaattgaa tcgattgatc aaagaagcag gattcgaaga acagcttgaa
    16981 gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa catgcgtccc acatcctgat
    17041 tctgaccatt tatcgattac tgagacagaa gtagacaatg gtacagtatt gcaaatcgtt
    17101 tgcggtgcac ccaatatcga agctggacaa aaagttgtcg ttgcaaaacc aggagctatg
    17161 atgccagatg gaatgatgat ctggccagga actttgcgag gggtagatag ctatggaatg
    17221 atctgttccg ctcgtgaatt gcatttgcca aatgctccag caaaaaaagg aatcttagta
    17281 ttgcctgagg atgccgtagt cggagaagca tttccaaaat aaagaggttg tgacaagtct
    17341 ttgtcacaac ctctttttta ttaattttca ctattttgat tttgttttaa tgccgattga
    17401 tcgatcgtca attcaacagt agttgttttt tctttcgagc cttcatagta agtgattttg
    17461 acagaatctc ccactttttt cttatacaaa gcagattgaa gttctacacc agaagaaact
    17521 tctttgtcat cgatcttagt aatgacgtcg tattgtttca atccagcttt ttcagccggt
    17581 gtagctgttt ggacatttgt cacgatgaca ccgttagtta cagatgaagg aatcttcaag
    17641 atttgttctt gttgctgagt agaaacggca gataagtcga ccattgtgat acctagagct
    17701 ggacgtgtca cttttccgtc tttttccagt tgattgatga tattcactac atcgttactt
    17761 ggaatagcaa atcccattcc ttctacgctt acattagaag tagattcaga agtacttgcg
    17821 atcttacttg agttgatacc gataacttgt ccttcgatat taactaaagg accaccagaa
    17881 tttccagggt tgattgctgc atctgtttgg atcgcattga tattgactgt ttcgttcgat
    17941 tcattcgtac ttgtcacttg tcggttcaag gaagaaatga ttcctgaagt gacagaattt
    18001 gcatattcag atcctagtgg ggaaccaatc gcaatcgcag gctcaccaac cttcagagca
    18061 cttgagtcac caaaagaggc aactgtttcg actttgtctg aattgatctt caacaccgct
    18121 aaatctgaat aagcatctgt tccaactaat tcagctttta ccttcgtacc gtctttcatc
    18181 aagacttcaa gaccttgctg gccatctact acgtgattat tcgtgactac ataagcagta
    18241 ttcccgtctt ttttgtagat cacgccgctt ccttcactat aagcttccaa attactatca
    18301 tctgagctgc tactttcttc ttgctgcccg aatagtccgc caaaaccgct tgattggttt
    18361 tgactttgta agttgataac agaaacgaca gcgtcttgaa ctttatctac cgctttggtg
    18421 atatcagaat ctacattgac tttgacattt tccacaactg tttcaccggc agaattctga
    18481 ttgccgcttg tagcagtaga actattgttg ccagtgccca tcgctgcata aaaaatgcca
    18541 gctgtcagta gtcctccaac gatccctcca acaagcccca gacctaactt acgccagagg
    18601 ctattatttt tacttttttt catctttggt gtcacatttt ttctatccat gtgttcctcc
    18661 tccaattaca ttcatttatc tactgttagt atagtcttat ttgttgactt tagtctactt
    18721 tgagactgtg aactttttat gaaaaacttt aggaaaaatc attgttttca gatgaaaata
    18781 tttgattttc tttttccaca gatactgtgt aaaactacgc ggataggatt ggaaaaataa
    18841 gggataaaac atatccacag cctgtgtata aagtggataa attcttataa aacgtatgtt
    18901 tgcctaatga atgtgatgaa ataaggtaaa ataacactgt tttctgtgga taaacctgtg
    18961 gatgttgtgc ataactgttg gggaaatctt tttaacaaaa atagccaaaa taaacatata
    19021 gacgaaaaaa ttgattataa taaacaaagt gagcatgaag accaaaggag agaaaacatg
    19081 aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat acctgatcca aggtatcaat
    19141 gagtatgtca aacggctgaa tgcctatgca aaaatcgaat tgatcgaagt tcctgacgaa
    19201 aaagccccgg agaatttaag cgaagcgcaa atgagacaag taaaagaaaa agaaggggaa
    19261 cggatcttag cgaaaatcaa agaacaagaa tacgtctatg ccttagcaat tgaagggaaa
    19321 aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat taggtatcca aggaaaaagc
    19381 caccttgttt ttgttatcgg cggctcttta ggactcagcg aagccgtcat gaaaagaagc
    19441 aatgctcaga tctcttttgg aaaaatgacg tatccacatc aattgatgcg tctgatcctt
    19501 gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg cgccttatca taagtgatgc
    19561 ggtttttatc attgatccta aaaatcttag caagacaaaa agtacgtaag aaagtaaaga
    19621 ctaaatactt cggaggaaca aatgaataac aatgataggt tattgcgatt gcgttatgcg
    19681 attgatttga aagatacgga tttgattaaa gcttttgagc taggcggtgt accactgact
    19741 aaagaagaag ctcaggcggt actgaccaaa gtacaggata agaacaaaga taatgcagaa
    19801 aataatgtgt atgaaaaaac catcaacaac caagtttttg acgcattcct aaatggcctg
    19861 attctcttgg cgcgtggtcc gcaaaaagat aagccgatag tagacacgcc gcaaaaaagc
    19921 aaggaaatca aatatatcaa caatgtgttg ctaaaaaaat taaaaatcgc cttgtctatg
    19981 accactgatg atattttaga cgtttttgct gaggcagaaa tctatccatc aaaaggcgaa
    20041 atcggggcat ttttgagaaa agaaggccaa cgaaatttta agccttgcgg agataaatat
    20101 atgcgtaatt ttctcaaggg gttaggaatt tataatcgga gaaaagtata aagaaatttc
    20161 atgagaaatc aaaacaatgg ttgttttgat gagagctagt ttttgtatga gattaaaata
    20221 ttatcttaat cgaggagctg gattggtttt atgaatgatg aatataagaa tgatgaagat
    20281 aagatgcttt ttgaagagat agagaatcga tgtaggctca attttgagct ttggggaaag
    20341 atgtctttaa tacaacaaaa aaagtattta gcgaataaat cagagtttac tctgggacat
    20401 gttgaaaaat taatctcgga ttggattagt agtagaagcg aatttacaaa aataaaacag
    20461 ccgattaaat ttgacatgaa aaaacttttg ttaaataaga gtgagatagg taatagagat
    20521 caatatatta gagctaaagg gcaagaaata attgattcat taggtgaaat gaggtcatac
    20581 aattatttgt atgtaacaca tagagctgat ggaatggtca ttactgtggg taaatcttct
    20641 tctaatgata tttttttaga tggagatctt ttttatcaac tcaatacaaa tcacttgtct
    20701 ggcactgaaa atataatttt aaggacggaa tatggcaatg aaatatttgc aaaatatgat
    20761 gagattttaa aaaattattt ggactgggct tggataattc cggttgagtc aggagatgct
    20821 aagaaactgg agagattatt gggcgatgaa ctaattaata aaaaagtacc aattttaaat
    20881 tattattccc atagacaata aaaggctatt tttcatgata atactagtta taactgtaaa
    20941 aatggaagaa aatctgggaa agaagttttt aaataagtgt attgttcgat tttaataaat
    21001 aataagcaat accatccgtc tgtgttgcgc aacatcgtac tacaatgaat attttttcac
    21061 aaaaagaaaa tcatccatcg aatgaatgga aggttttagt atacacgagg ttaatgcccc
    21121 ctcttttata atcagaggag tgaggttttt tctatgaatg tttctatttt cttattgcta
    21181 gtctagtttc tcttacgatg aaattgggat tctattccag tttttattga tttgctgata
    21241 aaataacttg aatggtggat ataaacatga taaataagag aagaataaca aaacaaggtt
    21301 gtgaaatgat tctcaaatga atataataga aagaaaaagg aggaagaatc aatgaaaata
    21361 gcaattgcag gcgctggcgc aatgggaagt cgctttgggt taatgcttca ccaaagtggg
    21421 aacgaagtat tattaatcga tggctgggca gaacatgtcc aacaaatcaa agagcatgga
    21481 ttacaagcaa attttaatgg taaagaggta gaagcaaaac taccaatcgt tcttcaatcc
    21541 gaagtagaaa aagaagatca agttgatctg attattctat ttaccaaagc gatgcagctg
    21601 gaaaaaatgc tgcaggatat ccaatcatta atcaaaaaag atacagaggt cttatgtcta
    21661 ttaaatggta tcgggcatga agatattatt gagaaatttg taccaatgga aaatatctat
    21721 attggaaata ccatgtggac ggcaggtctt gaaggtcctg gtcaggtcaa attatttgga
    21781 agtggttcag tagaattaca aaatttaggt gatggaaaag aagcagctgc aaaaaaatta
    21841 gcagacaaat tgtctgaatc aggattaaat gctcattttt ctgacaatat tcactattct
    21901 atttatcgca aagcatgtgt gaacggaaca atgaacggac tatgcacaat cttagacgtt
    21961 aatatggctg agctaggaaa aacatcgact gctcataaaa tggtggcgac gattgtcaat
    22021 gagtttgcca aagtagcagc agtagagaag attgaactag atgtcccaga agtcattgca
    22081 cattgtgaat cttgttttga cccggaaaca attgggttac attatccttc aatgtatcaa
    22141 gacttgatta agaaccatcg attaacagaa atcgattata tcaatggcgc aatttctaga
    22201 aaagggaaga aatatggtgt tgcgacgcct tattgtgatt tcttgactga gcttgtccat
    22261 gcaaaagaag atagtttgaa cgtaaaataa aggagagatt gaatggaaac ttcacaaaaa
    22321 atatcaccga aaatttttct gaataaagtc ctcgctggta cagcaacagg aatcattatt
    22381 ggattgattc ctaatgcagt attaggtgcc attttaaaat attttagtgc gtatcccttt
    22441 gcggtaacga tcgcacagtt agcagttatt tttcaactag caacaccttt gatcattggt
    22501 ggtttgattg ccttacaatt tggatttgat ccgatgagaa tgatggttgt agccggagct
    22561 tcttttgttg gttctggtgt agtcaaattc acacctgatt tgggtgaaac gggtgtttac
    22621 gttggtgctg gtacaggcga tattattaat acgatgctga cagcatcgat tgctgtcggg
    22681 ctgattttac tgattggaaa tagttttggg tcagtgtcga ttgtgttaac accgattgtt
    22741 gtaggtgtag gtgcaggttt acttggtttt tatttattcc cttttgtaac agcaattact
    22801 tctgcaattg gatcattgat taataacttc actaccttac aaccgctgtt aatgtctatc
    22861 ttgattggtt gttcctttgc ctttttgatt atctctccta tttcaacagt agcaatcgga
    22921 atggcaatcc aactagatgg tgtttctgct ggtgcggctg cgatgggcgt agctgcaacc
    22981 acttttgttt tagtagtgaa ctcttggaag gttaataagt caggtgtgac aattgcgatt
    23041 gctttaggcg ctatgaagat gatgatgcct aatctattta gaaaacctgt gattcttatt
    23101 ccttgtttag ttagtgcagt gattacagct attcctgtcg ctctattttc tatttcaggt
    23161 acgcctgctt ctgctggttt cggcgtggtc ggacttgttg gtccgttggc ttctttagat
    23221 gcagggctaa atggctttct tgttgttctt tgttggttag tcatccctgt ttttgcggca
    23281 ctaattactc aatttttatg cgagaaagta tttaaactgt atgatcgaga agaagtcttc
    23341 aaatttttag gctagtagat tttactaaga aattttcaat caatggatgc gtaagtgaga
    23401 tgtcaacaac atctgactta cgtttttttg cctttttgag taggagattg atgctgacaa
    23461 ctgaactctt cagtgccatt cgatttttta aaatgtagaa gaaactcacc tttatgttat
    23521 tgattgttca aaaggcaact agaataaaaa tatgtgttaa aatgagaaaa aatttgattg
    23581 ttggatgtat cgaataatca ctaaaaagta ggagaagaaa atgatttttt tgcaaaaata
    23641 tggtttttat tttttattat taggtgtgat tagtgattta tcgacaccgt atatccttgg
    23701 ccttttttat cccaaactga atcaaatgac taccgtaatt agtgtgtttg gtgacgtaga
    23761 tagtccagtc agacgtgctt tcttggtctg gtcagttgta tctggacttt tttttgttct
    23821 ttcgttgcct gcgttgtatc acctatttat tggaacatca aaaacacttg caatacttgc
    23881 tgttgcaaca gttggtttat atgggatagg tgattgtatt tttactgggt tattcagtat
    23941 caatacgaat gaaagctctt ggaatctatc gacttggatc cataatatag gttctgggct
    24001 aggctatgca ggctttctac ttttcccatt gttacttgtt ctgctttatc gtcaaagtgg
    24061 tcaaggtacg cttagccatt tttatcttgt tttaacagtt attagtttac taattgcagg
    24121 tctttacgga ttagcacgga ttccgtcgat tagtcagttt gccttcttta agcaattggg
    24181 cttctttcaa cgcttaagtt ttttctttaa ctatttgccg atgatttgtt taagtttttt
    24241 tcaactacga aagtagaaaa agcagtgtga agctgattaa gtaaaagaat ttctctatgg
    24301 gtaaccattc gtgtggatgt gttcgactaa gaatagagat gaggagagcg aaaagcaagt
    24361 tagtcttaac ttgttatgcg ttgtgctaaa tttcaggtag tcattttttg aatgaataag
    24421 aagaaaactt gaaagagggt gacttcatgt tagatgtgga gcagatggaa atagaccttt
    24481 ataatattat tttaaatcga aatacaagag aatgggaaag aacacaattt gaagaggcaa
    24541 aagaagacct agcaaaaatg tcgataaagc aggtgataga aaaactagag gtcaacttgc
    24601 gtccgctagc actacgagat aatttgacac cagatgcgat ggatttttat ttaagaatca
    24661 caggggagtc agcgagtgca aaggcttatg attatgccaa acataagata acttcaccct
    24721 tataccaaga acgggcgatt tttgccggtg gctgtttctg gtgtatggtt gagccttttg
    24781 aaacaaaacc aggtgtaatc tcagtgttat ctggttatac aggtggagag accaaaaaac
    24841 caaattacga tcaagtcagt ggtggatata ctgggcatgt tgaagctgtt gagatcatat
    24901 ttgatacacg tcaaataacc tatgaagagc tgttggagat ttattggcaa atttcagacc
    24961 ctacagatgc ctttggtcaa tttcaagatc gggggaaaca atatcgttca attctatttg
    25021 ttgttaatga aaagcaaagg caattggctg aaaaatcaag agaacaactg attcaatcta
    25081 accgatttag ccgtcctatc gttacagaga ttcgccaagc aaagactttt tggccagctg
    25141 aaaatcatca tcaacaattt tatcagaagc aacctaaaag atataaaaag atcaagcatt
    25201 cgcatcagct tcatttattt tttcagcgac atagatagag gaaggttgtg gcgcaaagcg
    25261 ttcagctccg acaaaataat gagcaactga caaaaatagc ttttcatatt tttggacagt
    25321 tatgaattat ttcgaggagt tgctttgaga acaccgagga aaaggttgtg gcgcaaagca
    25381 ttcagcttca agtcgcaagg aaacaggagg tatgacaagt cttttgtcac aactcctgtt
    25441 tttttactag cctttatttc catgtgaaag agaattcgaa ttgtagaatc ttgatttttc
    25501 tatggaattt tgtagagaat catcttgata attaatagac acctaatcga attccacatt
    25561 aatgatgaac taaattccgt ataaaagcgc aaacaataac t
//
LOCUS       DAJSMI010000020        25371 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJSMI010000020 DAJSMI010000000
VERSION     DAJSMI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.020"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            174..755
                     /locus_tag="ON430_000714"
     CDS             174..755
                     /locus_tag="ON430_000714"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR3218657.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            758..2065
                     /locus_tag="ON430_000715"
     CDS             758..2065
                     /locus_tag="ON430_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCR3218658.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2481..2969
                     /gene="purE"
                     /locus_tag="ON430_000716"
     CDS             2481..2969
                     /gene="purE"
                     /locus_tag="ON430_000716"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR3218659.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2962..4086
                     /gene="purK"
                     /locus_tag="ON430_000717"
     CDS             2962..4086
                     /gene="purK"
                     /locus_tag="ON430_000717"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR3218660.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4099..5394
                     /gene="purB"
                     /locus_tag="ON430_000718"
     CDS             4099..5394
                     /gene="purB"
                     /locus_tag="ON430_000718"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR3218661.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5574..5951)
                     /locus_tag="ON430_000719"
     CDS             complement(5574..5951)
                     /locus_tag="ON430_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218662.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            6207..6935
                     /locus_tag="ON430_000720"
     CDS             6207..6935
                     /locus_tag="ON430_000720"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR3218663.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            6935..7189
                     /gene="purS"
                     /locus_tag="ON430_000721"
     CDS             6935..7189
                     /gene="purS"
                     /locus_tag="ON430_000721"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCR3218664.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            7191..7862
                     /gene="purQ"
                     /locus_tag="ON430_000722"
     CDS             7191..7862
                     /gene="purQ"
                     /locus_tag="ON430_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCR3218665.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            7863..10085
                     /gene="purL"
                     /locus_tag="ON430_000723"
     CDS             7863..10085
                     /gene="purL"
                     /locus_tag="ON430_000723"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCR3218666.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSNAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            10070..11509
                     /locus_tag="ON430_000724"
     CDS             10070..11509
                     /locus_tag="ON430_000724"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR3218667.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            11532..12584
                     /locus_tag="ON430_000725"
     CDS             11532..12584
                     /locus_tag="ON430_000725"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR3218668.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            12581..13159
                     /gene="purN"
                     /locus_tag="ON430_000726"
     CDS             12581..13159
                     /gene="purN"
                     /locus_tag="ON430_000726"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR3218669.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            13156..14691
                     /gene="purH"
                     /locus_tag="ON430_000727"
     CDS             13156..14691
                     /gene="purH"
                     /locus_tag="ON430_000727"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR3218670.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            14707..15981
                     /gene="purD"
                     /locus_tag="ON430_000728"
     CDS             14707..15981
                     /gene="purD"
                     /locus_tag="ON430_000728"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR3218671.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            16103..17017
                     /locus_tag="ON430_000729"
     CDS             16103..17017
                     /locus_tag="ON430_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3218672.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(17150..18715)
                     /locus_tag="ON430_000730"
     CDS             complement(17150..18715)
                     /locus_tag="ON430_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3218673.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(18708..20159)
                     /locus_tag="ON430_000731"
     CDS             complement(18708..20159)
                     /locus_tag="ON430_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3218674.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(20173..21189)
                     /locus_tag="ON430_000732"
     CDS             complement(20173..21189)
                     /locus_tag="ON430_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3218675.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            21409..22497
                     /locus_tag="ON430_000733"
     CDS             21409..22497
                     /locus_tag="ON430_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCR3218676.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            22551..24077
                     /locus_tag="ON430_000734"
     CDS             22551..24077
                     /locus_tag="ON430_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR3218677.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            24079..25284
                     /locus_tag="ON430_000735"
     CDS             24079..25284
                     /locus_tag="ON430_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3218678.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
BASE COUNT         8053 a         4326 c         5822 g         7170 t
ORIGIN      
        1 gatgacagtt tctagccagt accgtaaatg ctggactata agtaaaagat tggctatttc
       61 acccttcttt tcttatgata aagtggaggg tgtttttctt taacagaaac atagtaggtt
      121 tttgcacaaa agcggttatt tcggtattaa taaatgataa aggagcaaat aaagtgaaag
      181 aattagttga acgtattcaa aaagacggac gtatattagg cgagggcgta ttaaaagtcg
      241 acagcttcgt cactcaccaa gtagaccctg tattgatgga acaaatggga aaacggtttg
      301 cagaagtatt taaagaacaa ggaatcacga aagttgtgac cattgaagct tctgggatcg
      361 caccagcttt atttgctgca agagaattag atgttccaat gatcttcgct agaaaagcaa
      421 aaagtttgac gatggatgaa gaattattga cagcttctgt ttattctttt acgaaacaag
      481 tgacaagtac gatttctatt tctcgtaaat ttttatcttc agaagacaaa gtattgatta
      541 tcgacgattt cttggcaaac ggacaagcgg caaaaggatt gatcgaatta tgccagcaag
      601 ctggagcgca agtagaagga atcgggatcg tcatcgaaaa gtctttccaa gatggacgtc
      661 agttacttga agaaatggga ttgagagtcg tttctcttgc gcgtattgct tccttgacga
      721 atggacaagt tgagtttctt gaggaggacg cataatcgtg gaaaaagaaa cacaaaatgg
      781 aaaagctgcg gttttagggc tgcagcatct gctagcgatg tatgcaggag cagtagcggt
      841 gcctcttttg atcggtacag gactaggctt taatcaagaa cagatgactt atttgatttc
      901 aattgatatc tttatgtgtg gattagcgac attattgcaa ttgacagtta accgattttt
      961 cggaattggc ttgcctgtcg ttttaggatg tgcgatccaa gcagtagctc cactgatctt
     1021 gatcggaacc aatgaaggag taggagctat ctacggatct attatcgctt ctgggatctt
     1081 tgttgtgtta gtttctggtg tcttttctaa aattaaaaaa ctattccctc ctatagtaac
     1141 aggaacggtt atcaccgtta ttggtttgac tttgattcct gtagcaatcg agaaaatggg
     1201 tggaggtaat gcaacagcag ctgattttgg tgacaaaaca aatctgctac ttgcatttgt
     1261 gacgattctc ctaattgtag gcgtgcaaat gctggcaaaa gggttcattc gttcaatcgc
     1321 ggtattgatt ggtttagtcg gaggaagcat cttagctgct ttcatgggaa tgattgatct
     1381 gagcgcgatt ggagatgcgc cgttgttcca tgttccacaa cccttttatt ttggtaaacc
     1441 aactttcgac gtatggtcga ttttactaat gatcatcatt tccatggtca gcatggtaga
     1501 atcaacaggt gtatattttg cacttggtga cattactggg aaaaatgttg gagaagaaga
     1561 attgaaaaag ggatatcgtg cagaaggact tgccgttatc ttaggcggta ttttcaatac
     1621 attcccttat actggctttt ctcaaaatgt cggattggtt caactttcgg gaatcaaaac
     1681 aagaaaaccc atctatttct ctgcattctt cttgattgtt ttaggattat tccctaaaat
     1741 cggtgcgtta gcacaaatca tccctgagcc ggtacttggt ggaggaatgt tagtcatgtt
     1801 cgggatggtg gcagtccaag gaatccgtat gctgttggaa gttgatttca gcaatgataa
     1861 aaatctattg attgttgctg tttctgttgg atttgggctg ggtttcaatg ttacgccgac
     1921 attattcagt caaatgccgc aaacattgca aatgttcact ggaaatggca tcgtaatgag
     1981 tagcattacg gctatcatat tgaatttgat ctttaatgga ttgaaaaaag atgaagaaat
     2041 attggaagaa gccttcaaga agtaatagag caaaagaaag gttgtgaaat cagcgttttg
     2101 acctgattgt tagagaaaca aacggaaaaa tagttcctcg tattttgccg tttgtttgag
     2161 ctaataccga gggtcagctg attgaacacc gttgaaggaa gaggtcgtga gcctgccgtt
     2221 catctccgag tcacaaggaa caatttcaaa aaaaagcttc tcatgtttgt tgaatattgt
     2281 tccttgtgac cgaggagttg gcaggagaac accgtttatc cataaaaagg aatgtgacct
     2341 aacttttgtc acattccttt tttttatctt taataaacac gaacaataaa atgtttttct
     2401 ttgttaacat tcgaaatttc tttgacatca aaaaaagtga ttgttaaaat atgaatgtag
     2461 aaaaactggg aggaaaaaca atgacagcaa aaatctctgt gattatgggg agtacttcag
     2521 attgggaaac aatgaaagaa gcatgtcaaa tattggatga attcggtgtg tcttatgaaa
     2581 aaaaggtcgt ttctgctcat cgaaccccag atttgatgtt tgaatatgca caaaacgctc
     2641 gaaaaaaagg aatcaaaatc attattgcag gagcaggggg agccgcgcat ctgccgggta
     2701 tggtcgcatc aaaaacaact ttgccggtta tcggtgtacc ggtccaatca cgaacattaa
     2761 atggtttgga ttcgcttctt tcgattgtcc aaatgcctgg aggagtacct gtagctacta
     2821 cagcaatcgg aaaagcggga gcaattaatg ctggactact tgcgatacag atgctttcca
     2881 tgtatgattt agaattggag gctaaattgg ctgaaagacg ttcaatgttg gctaaaactg
     2941 ttatagaaag tagtgatcaa cttggttaaa cctttgttgc ctggttcaac gatcgggatc
     3001 gtcggtggcg gtcaattagg tcgtatgatg gcgattagtg caaaagaaat gggctttaaa
     3061 gtaggtgtgc tggatcctgt gagcgattgt ccagccgcac aagttgcaga ttggcacatt
     3121 gaaagtgtct atgatgacac ctttgcgctg aaagagcttg ccagaagaac agatgtcatt
     3181 acctatgaat tcgaaaacgt cagtgtagag gcgttaaatg cgattttacc catgtcattt
     3241 attcctcaag ggacagactt gcttgcgatt acgcaagacc gcctgttaga aaaatctttt
     3301 ttggaaacga ataacatcgt gattgctcct tatgcaacga tcgtcagtcc gacggatatc
     3361 caagatgcga ttgacgggat cggctatcct tgtgttttga aaaccactcg aggcggctat
     3421 gatggcaagg ggcagtatgt tttgaaaagt cgggcggatc tagctcctgc catggacctt
     3481 ttaagagaag gaacctgtgt tttagaagca tggatcccgt ttgaaaaaga gatttcgatc
     3541 atggtggcag gaaatggtca aggagatttc acgacttttc cggtcgtgga aaacatccac
     3601 cacaacaata ttcttcatga aacgattgct ccagccgcga ttgatcagga tgtgatcgag
     3661 gaggctgagc gtattgcccg tgtgattgca gaagcagtga gcctttcagg tgttttaggg
     3721 gtagaaatgt ttttaacaaa aacaggcggg ctatatgtga atgaattagc tcctcgtcct
     3781 cacaattctg gacattattc tattgaagct tgttctatga gtcagttcga tgcccatatt
     3841 cgaggtattt gtggttggcc attaggagaa aaagtacgtt tgataagcaa tgcagtaatg
     3901 gtcaatgttt tgggagaaca actgctggaa agctatcgct tgattgagaa taagccagac
     3961 tggcaattcc atttctatgg aaaaaaacaa gcaaaaaaag gtcgcaagat gggacatatc
     4021 actatcttga cagaagacat ccagcaaacg ctggaagaaa tcaaacaaac gaatatttgg
     4081 gattagagga gaaaaaacat gttagaacgt tatacacgcc ctgaaatggg gaatatctgg
     4141 acggaagaga acaaataccg tgcgtggctc gaagtcgaga ttttagcaga tgaagcttgg
     4201 gcagaacttg gtgaaatccc gcaagaagat gtgaaaaaaa ttcgtgagca tgcatcattt
     4261 gatatcgatc ggatcttgga aatcgaaaaa ttaacacgcc atgatgtcgt tgcgtttaca
     4321 cgagcggttt ctgaaacatt gggagaagaa agaaaatggg tccattatgg tctaacaagt
     4381 acggatgtcg tagatactgc ttatggctat ctcatcaagc aagcaaatga tattttacgg
     4441 gaagacttga aacgttttac ggaaatcatt ggcgaaaaag caaaagaaca caaatataca
     4501 gtgatgatgg gtcggacaca cggtgtccat gctgaaccaa ctacttttgg attgaaactt
     4561 gccacatggt attcagaaat gaaacggaac atcgaacgat ttgaacatgc ggcaaaagga
     4621 gtagaagcag gaaaaatctc aggagcagtg ggaacgttcg caaatatccc gccatttatt
     4681 gaagagtatg tatgtaaaca attggggatc cgtccacaag aaatctctac tcaagttctc
     4741 cctcgggatc tacatgctga atatttctca gcgatggcgc ttattgctac aagtattgaa
     4801 cgttttgcaa cagagatacg cggattgcaa aaatccgaaa cacgtgaagt agaagaattt
     4861 tttgcgaaag gtcaaaaagg ttcttctgct atgccgcaca aaaggaatcc gatcggttca
     4921 gaaaatatgg ctggtctagc cagagtggtg cgcggacaca tggtcactgc ctatgaaaat
     4981 gtcacattat ggcatgaaag agatatttct cattcttcag cagaacgaat catcttgcct
     5041 gataccacga ttttattgaa ttacatgtta aatcgttttg gcaatatcgt taaaaatcta
     5101 acggtctttc ctgaaaacat gaagcggaat atgtctgcaa catttggttt gatttatagc
     5161 cagcgcgtta tgctgaaatt gatcgataag ggaatgacaa gagaagaagc ttatgatctt
     5221 gttcagccaa aaactgcgta cgcttgggac aaccaagtgc agttccgccc gctgttagaa
     5281 gaagatccaa ccatcacatc ggtattatct aaagaagaaa tagacgatgc ctttgattat
     5341 catcatcatt tgaaaaacgt tgacgtgata ttcaaacgag taggtttgga ataaagaaga
     5401 gtgagttgca aaattcaatt aaaatagaat agcaaaaata accaaaaaag aagcagatcc
     5461 gttagcggtt ctgcttcttt tagtttatta tattttctta tcaataatag gaacctgatt
     5521 ttaaaagcgg ctacctcttt accgtgcctc tttacataca aaaaatagag gaattaagcg
     5581 taaatggctt ggctattata actaaaatgc tggttatgga gatgttgcag caagttttcg
     5641 agaaaagcgt attccatctc ttgtccattg aataagatct ggatacgata atgatttttg
     5701 aaacgcttct gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc tgtctgataa
     5761 agaaaagtcc ttttacggca ttcagcacag cgcttggttg ctcggcggat ttttcggaat
     5821 gtataaaaac tagtatcttt attgcataaa taaacgtctt tgatcgacag caaatcatta
     5881 tatagacagg aacgttcttc agctgttaat tctgataaaa aaagctgacc ttgttttatt
     5941 gcatcttcca tcggcaagtg cctcctcgaa cccattatcc aaatttttag ttaattctaa
     6001 tataccatca ttaagtataa atgtaaacgc ttgttgatgg tataagattt tcgtttctga
     6061 taaaacacga acattaaaag aatctaatgc cttaaatgta catatttttg ttgatttata
     6121 aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt atttgctttt
     6181 aatgtaaata aaaaggagag acgtacatgg agaagaaaga acttttgtat gaaggaaaag
     6241 ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat ttagatcaag
     6301 caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa ttgaataatc
     6361 aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat cattttattg
     6421 aacaattgtc taaaaccgaa caattagcaa aaaaagtaca aattatccct ctggaagtgg
     6481 ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc gaaggtaaag
     6541 aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat gatccattta
     6601 tcaatgaaga gcatatctat ttcttacaac ttgcaaagcc ggaagaaatc agtgagctga
     6661 aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag gtcggtttgg
     6721 ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg cttgcggatg
     6781 aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat atggacaaag
     6841 acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta tataaccgtc
     6901 tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag tatatgtaac
     6961 ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag tccatcgtat
     7021 gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac aacttgctga
     7081 tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc ttgcaaatgt
     7141 aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac atgaaatttg
     7201 cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca gttcgcgaga
     7261 tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa ggatttgatg
     7321 gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt gcgattgctc
     7381 ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa ccagtattcg
     7441 gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt gtactgagac
     7501 gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac cccaagacgg
     7561 cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct catggggaag
     7621 gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat cagattgcct
     7681 ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt acgaacaaag
     7741 cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac ttgctaggat
     7801 cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag gtgaccgtat
     7861 agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga atctatgccg
     7921 aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta ggaagaatgc
     7981 ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt tcctataaaa
     8041 attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg caaggccctg
     8101 gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc aaagcagaaa
     8161 gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga gttggcggaa
     8221 tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat tcattgaggt
     8281 tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt gcaggtatcg
     8341 gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca tttgatcctt
     8401 gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa cacaaacata
     8461 tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt gcaaaaacag
     8521 gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa gaagaagaac
     8581 agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg cttgaagcgt
     8641 gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat atgggagcag
     8701 caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga cttatattga
     8761 cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg atgttatcgg
     8821 aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa atccaagaac
     8881 tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac gacgggcaat
     8941 atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca ttagctgaag
     9001 atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa tttcagcagt
     9061 tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat ctgctgcagc
     9121 aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa gtacaaacca
     9181 atacagttgt ccgtccagga agcaatgctg ctgtgatgag ggttagaggg acgaataaag
     9241 cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca aaaatcggag
     9301 gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt aagcccttgg
     9361 cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt tgggaattat
     9421 cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct gttatttcag
     9481 gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca ccaatgattg
     9541 gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc aaaaaatcag
     9601 gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt gaattacaaa
     9661 agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc attgaaaaga
     9721 gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt gctcatgact
     9781 gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat cagctaggga
     9841 tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa tcaagatttg
     9901 tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga gatgctgctg
     9961 ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact gacggtcaga
    10021 tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta tgtcttatga
    10081 agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc cagacgccgc
    10141 tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag gtgcaggtat
    10201 cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc tttctgaggt
    10261 attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg gacatgtacg
    10321 ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat tcaaatttca
    10381 tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt cccttagaaa
    10441 agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga tattgatgca
    10501 tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg ctttgaatga
    10561 agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg ccgctttgga
    10621 cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat acgtgattgc
    10681 ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg tccagcctgg
    10741 tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga aggacacgca
    10801 gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa acatcgcagg
    10861 gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag aatcgccagt
    10921 aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa gcggttatgc
    10981 ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca ttgcccgaac
    11041 atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat tatctgctgt
    11101 acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg ttcggggtac
    11161 gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag tccatgtgag
    11221 gattgcttct cctcctttga aatatccatg tttttacggg attgatattc aaacgcgtaa
    11281 agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg gtgcagattc
    11341 tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa aagatgctcc
    11401 gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt tatatgacta
    11461 tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa agcagaccaa
    11521 gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta gatgtcgaag
    11581 ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa cgtttaggta
    11641 tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt caattaaaag
    11701 agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg gcaatcgaag
    11761 cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat gatatcttag
    11821 cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa aatgatccgt
    11881 tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga aatatggcgc
    11941 tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat tatgatctag
    12001 ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa acgatcaacc
    12061 caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg tactctcttg
    12121 ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg ccagaattga
    12181 aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct tccttgcttc
    12241 cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga gggtttgtag
    12301 aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt ggctcatggc
    12361 cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag atggagatgt
    12421 atgaaatctt caatatgggc atcggtatgg tcctggcagt cgaagaatcg aaagtaaaag
    12481 aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg aaagtgatcg
    12541 ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg ctgtttttgc
    12601 ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa aaggattgga
    12661 atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga aacgtgcaac
    12721 tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa aaaaagagta
    12781 tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg tattagctgg
    12841 atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa tcatcaatat
    12901 ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg cctttgaagc
    12961 tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag atactggacc
    13021 aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt ctttagaaga
    13081 aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa ttattgaaaa
    13141 tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac tgggattgta
    13201 gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg aggaacaaag
    13261 catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac agggtttcct
    13321 gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg tcttttagga
    13381 agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca accaatcgac
    13441 tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga tgtcgaaata
    13501 gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag tgcagcaaaa
    13561 aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt actttcagaa
    13621 ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc aaaagtcttt
    13681 cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg ggtggaagaa
    13741 aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg ttacggtgaa
    13801 aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata ttcgatcgca
    13861 gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga tgcagatgct
    13921 gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa acacatgaat
    13981 ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc gtatgaagca
    14041 gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga tctgccaaca
    14101 gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta tacacaagaa
    14161 gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga ctttgcgcag
    14221 gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct tgtacaagat
    14281 caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg taagccgact
    14341 ccagcagaag caaaagcatt agcgtttgct tggagagctg tcaaacatgt gaaatctaat
    14401 gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca gatgaatcga
    14461 gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg tgcagtttta
    14521 gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa acatggtata
    14581 aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga tatggcgaat
    14641 aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta gagaggagtc
    14701 ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat aagtaaaaaa
    14761 cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc ggggatgaca
    14821 aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt aatccatttt
    14881 gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt gttaaatggg
    14941 atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa agcagcagct
    15001 ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca aatcccgaca
    15061 gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa agaaaatggt
    15121 gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt cgtggctacc
    15181 acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg atttggggat
    15241 agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc ccttttggcg
    15301 tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa gcgggcattt
    15361 gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt accgcaaatt
    15421 tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc tttgggaatg
    15481 aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc tacgaaggaa
    15541 ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca agtcgttctt
    15601 tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa tcgtacagta
    15661 gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc tgccggttac
    15721 cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga gctttctgtt
    15781 tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg acgtctttat
    15841 ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta caccgcgtta
    15901 atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa agctgccaat
    15961 agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca acaaacataa
    16021 tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact tttgttgtgt
    16081 atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg atcagaaagc
    16141 agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag taaaagtgat
    16201 tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt atggaaacgg
    16261 tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac gagaagatct
    16321 ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt ctaaaaaaca
    16381 tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg tggatgcttt
    16441 acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag cgtttgatga
    16501 attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac caatgcaaat
    16561 cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc aatttggctt
    16621 gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg aagcaagtat
    16681 cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga tccaagcatg
    16741 gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa aattccctga
    16801 attaaaccaa acattagaag aactagcagc taaatatcaa acgacaccaa ctggcttggc
    16861 ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta cgatgaacgt
    16921 agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg aagattggta
    16981 cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag caggtcaaaa
    17041 gggctataaa ttaaaaagac agaataactt gaaagctgtt ttcagcttcg agatattctg
    17101 tcttttttta ttgcaaatgg aaagagtatc attgtacgga tttcgtttat tagttatttt
    17161 tgtttttaag gaaaagctgt gtccgatatt cttttggaga aatcccttgt tttttcttaa
    17221 ataaataact gaaatagtta gggtcattat aaccaatttc aaaagcaata tctgaaatac
    17281 gatagtctga ttgctgcaat aatttttttg ctaattcgat tctttgttct gtcagataat
    17341 cgatgaatgt ttggttaaac gattgagaaa aaatcgtact gaaatgagag ggacttaatg
    17401 caacttgttg agcgacccaa gttaaggaca tatctggatc ggtaaaattt tctttgataa
    17461 aatccaatgc atggttcaaa attggctgat atttgttcct tgaaggctgt gagggcagag
    17521 cagttaagaa agaaatgaac atataaactg tcgaacggta aagcgagtcg tcggcgctga
    17581 ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt ttcttttctt
    17641 tcaggcagac atgctggagt agcttagtca agacaaataa acgatataat cgtgttcgag
    17701 ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg tttttcgata
    17761 atgaagacaa atcaagtgag aggtcaaagt aaaagacaga atcaactgga agtttcgctt
    17821 cttgaatcaa atcctcatag ctgataattt gttctttaag ggaactggcg gtttcaagta
    17881 tcatctgcgt gatctcaaaa gatgcaggta attcgctgat tctttctgcg acaggaccaa
    17941 tggcgacaga aaaattattt ttttgtccct tttctaattc atggaccaac gtttgggcta
    18001 cctgataact ggtctcgatc acattttctt caagagaatc aactattaaa aatttgatga
    18061 atcgagagga aacagcagag aagatcactt gttgattctc cctaaataaa aacttcaaat
    18121 aatcactgag atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct gctaataata
    18181 cggtcatttt tttccctgct aaagagcgat tgaaagattc agcaagtttc aatgtttcac
    18241 tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg attttttttc aattcgaaaa
    18301 caagatcaga agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt actgcttttt
    18361 ctattgcttg tttcaattcg ctacttttaa tcggtttaag cagataatca tctgcctgta
    18421 cctggattgc tcctttcata taatcgaaat cgtcgaatcc actaataaat atgagacgag
    18481 tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg aaaggcattt
    18541 ggatatcagt cagaatgatg tcgggctgta agtcgataat tgaagacagt gccatctcac
    18601 catctccagc ttcgccgaca taagtgattg gataataaga accaaggaca gttatttgtt
    18661 cccgcaagtt attgcgaatg aggtattcgt cttcgatgat aaaaacttta tacatgtttg
    18721 tttcctccaa ttgttttggg agatagaggg atcatcagtg tgacagttgt tccttctcgg
    18781 tatttgctgt caatcgtgat aggtttttga tcatttccgt aataaagaag gagacgcttg
    18841 tttacgttgt aaagtccgta gccgatactg aaatctgaat cgatcccttt ggctaactct
    18901 ttacgtattt ctgccagttt ttctgcaggg atgccaatcc cgttgtctat aactttgaat
    18961 tggatcatct catcaatttt gcatgtggta atacgaataa atcctttgtg acggacgtac
    19021 ttcgttccat ggtatacagc attttcaatc aatggctgca agatcatttt taaaatagaa
    19081 acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc gtatcttatt
    19141 tttaagatga ctagataatc attgatatgg ctaatttctt tttcaagagg aatccagtct
    19201 tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat agtcagagct
    19261 tcttcaaagc agtcttgttc gatcaaagaa ataatggcat ccaaactgtt ataaataaaa
    19321 tggggagtaa tctgagcttg caatacacga acttcacttt gtgctaaatg gtattgtttc
    19381 aaagcgttct cccgtaaaga ctggtttaga tcttccgcca ttttattcat gctagcggta
    19441 agtgtttgta attccagtgt ttccggcagg gttaaacgaa agccaaggtt ccctgaagaa
    19501 agttgttctg acatctcgat cagctgattg atcggttctt ggacctttct ctctaaaaag
    19561 cgacgattct ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc cactattgtt
    19621 aatagaatga gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc gatctccaca
    19681 cgaacaaaat cttgtaagat atctgacagc agttgagtga cagaatctac ctgattgatg
    19741 atctcttcat tggtatccat aaaatctttt gccaagatat tattgatcag ctcgttttga
    19801 taatcttcca gtgtatcgat cgtacgtagc gatacatcta acgtactaat ttctttaagt
    19861 gtatttgtat tatttttgat ctctcgaata tcattcctaa gttcttctag gatattttcc
    19921 tcattgaact catctacggt tacttggcca gaaatcaagt cccacatact ttccaaaact
    19981 ttttgatcaa ccttcaaagc aattgcatct gctttttgga tatttgcgat cgtctgttga
    20041 taaatcaaaa gctgttgcga atataacagt atgctcagta gcaaagggat aacagcaaat
    20101 agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt ttttgtcatg
    20161 atacttctcc agtcaataat ttctggttgg gatgttagct atattctgct gggaaaagac
    20221 agtctcggaa ataaaaatat ctttaggtaa tgtttttcct gtttgaatat ttccaaaata
    20281 acgagtaacg gtattggcta cataccagcc gacatcaggt ttacactcga ctacacaatt
    20341 gacgattcct gagcgtagct tctcgatcat atcttcttgt ccatcaatag ttacaatgat
    20401 caaatcttct ccaggtttga ttgatgtttc ttttatcgca gctaaagcac cgtgagtcat
    20461 ttcatcacta tggctgtata atacatcgat ttcggataaa tcatttgttt ctaaatatcg
    20521 tttcatctgg tcttttcctt tcatccgaat aaaatcaccg acaattcggt gaacaatagt
    20581 catcggaggt ttatggtcga tggttccatc tttttttagg gttggtttac gattgattga
    20641 ttccaaaaat ccttcggagc gtagcttggt cggtgtggag ttctctgaac cagcaagttc
    20701 taggattctg atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa ataaacccgc
    20761 ccgatttcct tctgctttga aactggggcc aatgtgggtg acgaaaagct cgggatcatt
    20821 cgtttgaata tgtctgtcga caagtatgac agggatccct gctcgctttg cttcttttaa
    20881 tacagagtcc cacccggatt cttgcaaagg agtaaaaatg atcatatcca ctttataggc
    20941 aatgaacgtc cgtatatctt ggatttgccg ttcctgattc atgtagccat ttcggtagag
    21001 aacttggtaa tcttcttttt caagcgcagt gataattgat tcagtatgct ttttccgcca
    21061 gctactctcc gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg cttcctttgt
    21121 attaggtgta caccccatca tttggtaagc gattacaaca agggctaaaa aaaataaaac
    21181 acgtttcact ttaaatcact ccatctggat ttgttagtac tatcttatca gataaaaggg
    21241 aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa aaatccaaga
    21301 caaaaaaagt acacctaaaa aatttaaagt aaatcccata aaaaaatcaa tgttaacgct
    21361 ttctttatta gctatactcg gtgtatcaat tgaaatgggg gataaaaaat gaaaaacaaa
    21421 tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa gcattctatt aagtgcgtgt
    21481 ggcaattcag gatctgcaga tgatagtacg gggtatgtag gaatcgctat gccgaccaaa
    21541 tcagcagaaa gatggatcgc tgatgggaac aacatggtct cggaattaga aaaactgggt
    21601 tataaaacag atcttcaata tggagaagat aaagtagaaa atcaagtagc acaaatagaa
    21661 aatatgatta caaaaggggt agatacactt gtgattgctt ctatcgatgg ttcggcttta
    21721 actgatgtgt tagctaaagc aaaagaagca gatatcaagg tgatcgccta tgatcgttta
    21781 ctgatgaatt cagaaaatgt cgattattac gcgacttttg ataattttgg agtaggtgta
    21841 tcgcaagcag cttatattga agagcactta ggattgaaag aaggaaaagg accattcacg
    21901 attgaattat ttggtggctc gcctgacgac aacaacgcac taatcaatta caacggagtg
    21961 atgtctgttt tacaaccata tatggacaac ggtcagttgg tcgtgccatc tggacagacg
    22021 agtttcagtc aaatcgctac gcttcgctgg gatggttcta cggcgcaagc aaggatggat
    22081 aatctgttaa gcgcaaatta tactgaccaa acattggatg ctgttttgtc gccatatgat
    22141 ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag tgaaagtaaa
    22201 cctttacctg tgattactgg acaagatgcg acagttgctg gcgttaaatc gatcattgcc
    22261 ggggaacaaa cacaaacgat ctttaaagac acacgtatct tagcaaaaaa tacgattgaa
    22321 atgatcaaag caatcagtga tggagaagag gttccggtca atgatactga aacttatgat
    22381 aatggggtga aaactgtccc tacgtattta gcaaatacag tatctgtcga taaagataat
    22441 tatcaagcag agctgatcga caccgattat tataaagaat ccgatctgaa aaattaagag
    22501 gaaccaaaca aggaactaaa gagatctaag tcagagagag gataagaatt atggcggatt
    22561 gtattttaaa aatggcagat atcaccaaaa aattttctgg aatccgagcg ttagattgcg
    22621 tgaatctgat gattgaacgt ggggaaatcc atgcattgtg cggagaaaac ggagcgggaa
    22681 aatcaacatt gatgaatgtc ttgagcggct tgtatccata tggctcctat gacggggaaa
    22741 tcttttatga tggaaagctg tgtcaattta aagatttgaa agatagtgaa gaaaaaggca
    22801 tcgtcatcat tcatcaagaa ttagcactaa gtccttatct ttcaataaaa gagaatattt
    22861 ttctcggaaa tgaacaagca gaaagaggcg tgattgattg ggaactgaca gaaaagaaaa
    22921 catatgaact gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta gtatcccaga
    22981 ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa tccgtccgtc
    23041 tgttgatttt ggatgagcct accgctgcat tgaatgaaga agaaagcgcg aatttgctga
    23101 gattgatccg agagttgaaa gagcaaggtg taacctccat cattatttca cataagctga
    23161 atgaaatcgt ggatatcgca gatcgaatca ccattttacg ggatggacag acgattgaga
    23221 cactagaaaa tgaaatgatc agcgaagagc atattattcg cggaatggtg ggcagagatt
    23281 tagttgatcg ttatcctgaa agaaaaccgg atattggaga agtttatttt aaagtagaag
    23341 actggacggt ctatcaccct cttgatcacg agcgaatcat aaacgaccac atcaacttgc
    23401 atttgaaaaa aggagagatt gtaggaatag ctggactgat gggagcgggc agaactgaat
    23461 tcgctatgag tctatttgga cattcttatg gaagcaagat atccggacgt atattaaagg
    23521 aaggggcaga gatatcagtt aagaccgttc cttcagccat tgcaaacgga ctcgcttatg
    23581 tctcggagga ccgaaaatct ttaggactga atctgctgat ggatattcgg gaaaacgtga
    23641 cgctgagcag tttggataaa ataagtcaaa aaggagtatt agataaagaa aaagaagtaa
    23701 ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac aaactccatc catcaaattg
    23761 tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg ctgatgacac
    23821 aacccgatat tctttttttg gatgagccaa cccgtgggat agatgttggg gcaaaatatg
    23881 agatctacac gattatcgaa gaaatggcag caaatggcaa agctgtctgt cttatctctt
    23941 ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta tacgatgaac gaaggacgta
    24001 ttacaggaga agtctcgcga gaagatgcag atcaggaagt cttgatgaaa ttaatgacga
    24061 aagaagaggt gggttaagtt ggaacaggca aaaaatctta cggataaaaa agagaaaaaa
    24121 ctaaatttga aaagtagagc agctgatctt tttggtcgct acagcatggt gatcatttta
    24181 gcgggactac ttattgcgtt tcaattgatg acggatggta ttttctggcg tccattgaat
    24241 ataactaacc ttgttctgca aaatagccat atattagtac tagctgcagg gatgttatta
    24301 gttgtgctct taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt tgttggtgca
    24361 attgcgggca tgatgatggt caatcgccaa atgaacccgt ggatgacagt tatcgcatgt
    24421 cttgcagtag gcgggctgat cggcgcatgg caaggttttt gggtagctta tgcagggata
    24481 ccggcgttta tcgtgacttt agcaggattg ctgatgtttc gtggactgac acaagttgtt
    24541 ctaggaggcc aatctctagc accttttcca gctgttttcc aaaaaatgtc tacgggttat
    24601 ttaccagact taagcgaagg aagcagcctt cacttcttga cattgatttt aggaatgatc
    24661 atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa aaatcattta
    24721 tttgaagtgg aatctccaac agctttcttg gtaaaggcgg tatttacagc agtcgttgta
    24781 ctaggaataa cttatatctt tgcctcttat caagggtatc cgattatttt gatcattctt
    24841 ggtttgatcg ttgcagcata tgggtttttg acaaataaaa cagtagcagg tcgacagatt
    24901 tatgcgacgg gcggaaatcg aaaagcggct gaactatcag ggatcaaaac aaagcgaatc
    24961 acgttctggg tattcgtcaa catgggagtc atggcagctt tagctggatt agttttagct
    25021 gctcgattga atgcggcaac acctcaagca ggtacatcga tggaactgga tgcaatggcg
    25081 gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat gggtgcgatt
    25141 gtcggtggct tggtcatggg tgtattgaac aatggcatgt cgattttagg cgtcggggta
    25201 gactggcagc aggcaatcaa aggattgatc ttgcttttag ccgttgtgct agacttgtat
    25261 aataagaaaa aacgaacaac gtaggagaac gccggttatt cagaaaaaag actgtgacaa
    25321 gacatttgtc acagtctttt tttcgctaaa agcgaacatt cattgtttcg t
//
LOCUS       DAJSMI010000021        25072 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJSMI010000021 DAJSMI010000000
VERSION     DAJSMI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.021"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            109..678
                     /locus_tag="ON430_000736"
     CDS             109..678
                     /locus_tag="ON430_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3218679.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(856..1296)
                     /locus_tag="ON430_000737"
     CDS             complement(856..1296)
                     /locus_tag="ON430_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR3218680.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1454..2218
                     /gene="map"
                     /locus_tag="ON430_000738"
     CDS             1454..2218
                     /gene="map"
                     /locus_tag="ON430_000738"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR3218681.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2250..3173
                     /locus_tag="ON430_000739"
     CDS             2250..3173
                     /locus_tag="ON430_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCR3218682.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            3249..4388
                     /locus_tag="ON430_000740"
     CDS             3249..4388
                     /locus_tag="ON430_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCR3218683.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            4381..5181
                     /locus_tag="ON430_000741"
     CDS             4381..5181
                     /locus_tag="ON430_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3218684.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            5181..6008
                     /locus_tag="ON430_000742"
     CDS             5181..6008
                     /locus_tag="ON430_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3218685.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            5986..6720
                     /locus_tag="ON430_000743"
     CDS             5986..6720
                     /locus_tag="ON430_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3218686.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            6820..7686
                     /gene="rfbA"
                     /locus_tag="ON430_000744"
     CDS             6820..7686
                     /gene="rfbA"
                     /locus_tag="ON430_000744"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR3218687.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            7700..8272
                     /gene="rfbC"
                     /locus_tag="ON430_000745"
     CDS             7700..8272
                     /gene="rfbC"
                     /locus_tag="ON430_000745"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR3218688.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            8294..9322
                     /gene="rfbB"
                     /locus_tag="ON430_000746"
     CDS             8294..9322
                     /gene="rfbB"
                     /locus_tag="ON430_000746"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR3218689.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            9420..10271
                     /gene="rfbD"
                     /locus_tag="ON430_000747"
     CDS             9420..10271
                     /gene="rfbD"
                     /locus_tag="ON430_000747"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR3218690.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            10305..12338
                     /locus_tag="ON430_000748"
     CDS             10305..12338
                     /locus_tag="ON430_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218691.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            12382..13662
                     /locus_tag="ON430_000749"
     CDS             12382..13662
                     /locus_tag="ON430_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCR3218692.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            13872..14678
                     /locus_tag="ON430_000750"
     CDS             13872..14678
                     /locus_tag="ON430_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218693.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            14690..15910
                     /locus_tag="ON430_000751"
     CDS             14690..15910
                     /locus_tag="ON430_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218694.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            15900..17486
                     /locus_tag="ON430_000752"
     CDS             15900..17486
                     /locus_tag="ON430_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3218695.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            17525..19663
                     /locus_tag="ON430_000753"
     CDS             17525..19663
                     /locus_tag="ON430_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3218696.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(20031..21014)
                     /locus_tag="ON430_000754"
     CDS             complement(20031..21014)
                     /locus_tag="ON430_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR3218697.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTYH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            21392..22783
                     /locus_tag="ON430_000755"
     CDS             21392..22783
                     /locus_tag="ON430_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR3218698.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            22799..23839
                     /locus_tag="ON430_000756"
     CDS             22799..23839
                     /locus_tag="ON430_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3218699.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            23858..24943
                     /locus_tag="ON430_000757"
     CDS             23858..24943
                     /locus_tag="ON430_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR3218700.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT         8100 a         4113 c         5124 g         7735 t
ORIGIN      
        1 cagcttgtga agaaaatcaa aaactaattt tgcatatcaa aaaaacatgt tacaatgaaa
       61 ttaatcaatg gtggaagcgt tataaaaaaa ttaaggagat taaaaaatat ggcaagaaaa
      121 aagacgatta ctagagaaca gattttggca gctgcttatg aagtagctgc caccgatgga
      181 tttacacgat ttactgcgag aaacatcgcg aacaaaatga attgttccac gcaaccgatt
      241 tatcttgagt ttaaaaatat ggatgacttg agacaagctc tttttgagca gatctatgat
      301 catttacaat atgaagtgtt cactgttgag catacaggga atacgattat cgatcttgct
      361 tttaattata tccattttgc aagaagagag aagaagttgt atcgttcact gtacttagaa
      421 gaatatggtg gaggaaaaga gatgcagaat ttctcttata cttactttgt gaatacagtg
      481 aaacgcgatc cagcttatgt gaatctttca gaagagcaga tcgaatcttt gtataatggg
      541 acatggatca tagctactgg gatcgcagct ttgatgtctt caagcatcat ccatccaact
      601 gatgaacaga ttgaaaagat gatccaagac agtatagatg ctatcttgaa attcaaagaa
      661 ccaatcgaaa tcgattgaat atactcaaaa gttccacaaa tcagtaggta acaagagatg
      721 tgtcaaaata aggaaaagtt cgtttttctt acagcgtatg catttttaac tcccgaagaa
      781 ctcttccatg aatgcaatga ataaaagagc catacagatt gaataaatct ggatggctct
      841 tttattgcaa gtatattaaa tttttgcgac tagttcttta gcgaaagctt ctaaattttc
      901 gatatcttct tcttcagcat tcagatccac tttaacattt tcggctcctt ttgatgcgcc
      961 tgtttttgca aaagcagcat cgaaatcatc caccgatttg cagaattcat cgtaaaaagt
     1021 atcaccagat ccgcagacac cgtaaatttt tcccaataga tccaattctt gcaagtcttc
     1081 ataaaagtct acgatctcat caggcagatc gccatcatcg tatgtataag tagctactac
     1141 gcaaatatct gcctcctcga attcatctgc atctacttgg gtacattcgt tgatttcgac
     1201 ttcgatatcc agattttcaa atgcttctgc aacaatatcg gcgatttctt cagtgtttcc
     1261 tgtcatgcta gcatatacga tttttgctaa agccatgatg tgcctcctcg tttataatta
     1321 tttaacaatt atgaattata ccaaaacatc attgaaaaga aaataaaacc taaagacacc
     1381 tggggaatgt catttggcta aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa
     1441 aggagacgaa aatatgatta ctttaaaatc agcacgtgaa attgaaatga tggcagaatc
     1501 tggagcttta ttagcagatg tacataaaaa tctgcgtgat tttataaaac caggaatcac
     1561 aagttgggat attgaggtat ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc
     1621 gcaaattggc tttgaagggt atgaatatgc gacttgtatc agtataaatg atgaaatttg
     1681 tcatggattc cctcgtaaaa agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg
     1741 tatcgatttg aaaggcggca tttctgattc ttgttgggcc tatgtagttg gagaatctac
     1801 acctgagatc gaccgattga tggaagtaac gaagaaagct ttgtacatcg gtattgagca
     1861 agcacaagta gggaatcgga ttggtgacat cggtcatgct atccaaactt atgtagaagg
     1921 cgaaaatcta gcgatcgttc gtgattttat tggtcatggc gttggtccta cgattcatga
     1981 agaaccagca gttcctcatt acggagaagc tggaaaaggg ttgcggttga aagaaggcat
     2041 ggttatcacg atcgaaccta tggttaatac cggaacttgg aaaatgaaaa tggatcctaa
     2101 cggctggact gcgtatacaa gagacggcgg actttcttgc caatatgaac atacattggc
     2161 tattactaaa gatggtccaa gaattttgac atcacaagga gaagaaggca cttattgatc
     2221 tatccagtca ataggtacgg gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg
     2281 aaaatctgat gcgctttatt gagacgactc aaaagcgcat cactgattca gaaatgagta
     2341 cgacctctgt cgtcgttgct tattatttac tcttgtcttt atttccattg attattgctt
     2401 ttggtaatat tttaccgttt ctccacatcg atcaagagac tgtactgacc tatttacgag
     2461 aagtgattcc tgagaccgtt taccagttta tcggtcctgc aatcaaagac cttttgacac
     2521 aaagttcagg aggcttgtta tccgtttctg cattagcggc tttatggtct gcaagtcaaa
     2581 gtatcaatgc tttgcaaatt gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt
     2641 ttttgatcgt ccgtttcttc tcactaattg tcattgtctt gttcatgatt gctattagtg
     2701 gggtaactat cgttcttggg ttgggacagc tgattttgga tgctattcag ccaatcgtac
     2761 aaatacccgt gaactttatc gatcagttcc aaactttgaa gtggccaatc acattggtag
     2821 ctttatttgt catcatgttc ttgatttatt taattgtccc taatgctcag ttgaagttga
     2881 agacgatcat tccgggcacg atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt
     2941 ttagtatcta tgctaggtat tttgcaacga ggatcagcgg ttatcagatc ataggaagtt
     3001 ttattgtact catgctttgg cttaattttg ctgctacgat catcattttg ggcggaatca
     3061 tcaatgcagt tgttcaagaa tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa
     3121 gccgaaaatg gttcaataaa ttgaaaacta aattttctca taaaaaaaag tagaaacaag
     3181 agttcttatt gtaaatttat atcagtaatt tataataatt atgactctta attaaagtag
     3241 gtgcccatat gcttgtttct tttagtatgg ttataagatt ttttctgaca attttattat
     3301 cactgatatt aacacctgta ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac
     3361 caaatgaaag aagaataaat aaagttccaa tgccttcagc gggcggactg cctatcttta
     3421 tttcatttgt tatatcgact ttattcttat tccgtaaaat tatccctcaa tcgtatattg
     3481 taccgatctt gattgcttct ttagtaatca tagtcacagg agtgctcgat gataaatatg
     3541 agctgacacc taaacaaaaa agtataggga tcttgattgc ttctcttatt atttattttg
     3601 tagcagatat acgtattgat tccttcacgc ttccttttat tggatatatc caactaggct
     3661 ggcttagttt tcccgtaacg attttttgga tcttcggtat cacgaatgcg gtcaatctga
     3721 ttgacggttt ggatgggctg gcggcaggga tttcattgat cggtttaatt acgataggaa
     3781 tcattggata ttttttcctc catgcttcta cagtgtatat tccagttgtt attttctgtc
     3841 ttgtagcaag tattatcggt ttttttcctt acaattttta tccggcaaag atttatttag
     3901 gcgatacagg agctctattc ctagggttta tgatggccgt cctttcacta caagggctga
     3961 agaacgtgac ttttgtttca tcgatttctt tgctgatcgt catgggagtg ccggtaacag
     4021 acacgttttt tgctatcatt aggagaaaag caaaccgtgt ttctttttct acagcagata
     4081 aaaaacatct tcatcaccga ctaatcagtt taggctttac tcataaaggt gctgtcctga
     4141 cgatttatgc gatggctttg atgttttctt tcacagctat ggtcatgaat tatactggaa
     4201 gaatcggaac gatcatattg attattgcca tgttgtttgc tgcaatttta ttatttgaat
     4261 tgattggtct gatcaatgag aaacatcagt ttattttaaa aacattgcga tttttaggga
     4321 ataaagagta ccgtacgcaa gtaaaacaga aatatttgaa gaaatggaac cgtcatatag
     4381 atgaataaca aattaaacca gccgatcctt attagtattg tgacggcaaa cagtgataaa
     4441 atttttcgga cactagataa ttttatttca actgtaaaag atgaaacagc agtaaaaatc
     4501 cgaatttttg acaacaattc acttcccgag tacaaaaaaa gactaacaga gtataggaaa
     4561 tatccctaca tcgatatcta ttttcatgat gaaaattccg gatttggtta tggacataat
     4621 cacaatctga tagctagcga ttctcgctat ggcattattt gtaacccaga tattttagtg
     4681 gatgaagaga cagttacagc attggtcttg ttattgaaag aacatccgga atgtgcgatg
     4741 ttagcaccta agattcttaa tgaagatgga acaactcagc acctcatccg tgagcagctg
     4801 gctgtttttg attacgtttt gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac
     4861 aaacgactgg ctgtgtttga atgccgagga ttgcctactg atgctaatag ttatgtacgg
     4921 atgatctcag gtagttttat ggtagctgat attgataaat ttaaagagat aaaggggttt
     4981 gatgagcgtt tcttcatgta ctttgaagac aacgacttgt gtttgagttt tgaaaaagca
     5041 ggttacaagt tattgtacac acccttttat gcagttatcc atttttacgg gaagggtgcg
     5101 catcgaaatg caaaactgtt tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag
     5161 tgggggtggc gttttttctg atgaaaccag aaatattagt agtcgttgtg ctttatcagc
     5221 aactattcag tcaatctcca gcttatggac cattaagcaa agcacttagc gaaaagcaaa
     5281 ttcagcttat tatttacgat aatagtcctc aaaggcagca aaatgagctg tttgaaaaag
     5341 aaaatatcgt ttatcatcat gatccgcaaa atcctggttt agcggtggct tataattttg
     5401 cgttgtctca agcagatgaa ggaacacgtt gtctcgtcac tctggatcag gatacgaggc
     5461 tagtcgacga ttattttgaa ctattgcgta aagtaacctt tacagatgaa tgtgttgctg
     5521 cagttccgat gattttttct gggagccgac aagtttctcc cctatattca gatcgttata
     5581 tcaatcgtac agctcaagca gtagaagttg aaggcccaac agctgaacga atcatggcaa
     5641 tcaattcagg tgttgcctgg tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg
     5701 ctttttcttt agattttttg gatcattggc tgttttggaa agtggatcag ttggaaaaaa
     5761 ctgtggagat ccttcctgta cgaatggaac atgatctttc ggttttagac tatgaaaaag
     5821 tttcaagtaa tcgataccgt tctattttac aagcagaaaa tcgattttat catgaatatg
     5881 atcaaaggca tttatccagt caccgacgac aattattgtt aagaacagct aaacagttct
     5941 tgaccgtcaa aaaccggcat atctggcgga tgactttgcg ctcatatgtt aagaattgga
     6001 aggtgtgaat tcgtgcgttc ggtttgtata gctaccttta atggaggcag atatttaaga
     6061 gagcagttag atagtatttt atctcagctt tcagcagaag atgaagtagt catttctgat
     6121 gatgggtcag tagatgatac atgggatata ttaactgaat acgcagaaaa agataagcga
     6181 atccagcttt tttcggggcc tcgtcaagga ttgatcgcta attttggtta tgcgatccag
     6241 cagacgaaag gagatctaat ctttttggca gaccaagatg atgtttggtt ggagacaaaa
     6301 gtgaaaaaga tcactgacta ttttgagcag aatccggatc agctagtagt agtttctgat
     6361 ttaatcattg tggacagcga gctgcaagag atccatgatt cgtatttttc gtttcgaaat
     6421 tccaaagaag gatggcttaa taatgtgata agaagtcact atattggtgc tggtatggct
     6481 ttccggtctt ctttaaaacc cgatattttg ccgatcccgt cagaagtgcc gatgcatgat
     6541 atgtggattg gtttgctggg aggaaagaag actgggttcc taagagaacc gttaactctg
     6601 taccgaagac atgatttaaa cgcgagtgag attgaaacac attctaaatt acggcaaaaa
     6661 atttcttggc gtgttcatct gattacggcg ttgatcaaac gaaaatggtt taaacgttga
     6721 taaaagatgt ttaaatcctt aatatttcca tgacaaatat aacaattgat gagataataa
     6781 caaagtgaaa tttgaaatca aaaaggagag cagatatcca tgaagggaat cattcttgct
     6841 gggggcagcg gaacacgtct gtacccttta acaaaggcaa catcgaaaca attgatgccg
     6901 atttatgaca aaccaatgat ttattatcca atgtctactc tgatgttggc gggaatcaaa
     6961 gaaattctga ttatttcaac gccacaagat acaccacgtt tcaaggaatt atttggaaac
     7021 ggaaatgatt taggactgca gatcgaatat gcagtacaag aaagtccaga tggattggca
     7081 caagcgttta tcattggaga agaatttatt ggagatgata gtgtctgctt ggtattaggg
     7141 gacaatattt actatggtgg cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat
     7201 acaggcgcga cagtatttgg ctatcatgtc aatgatccag aacgctttgg tgtagtagaa
     7261 tttgatgaga atatgcaagc tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat
     7321 tacgctgtaa ctggccttta tttttatgac aatgaggtaa tttctatcgc taaaggtatc
     7381 aaaccttcag aacgtggaga actagagatt acggatgtca ataaagcata tttggataaa
     7441 ggaaaactat ccgtagaact tatgggacgc ggatttgctt ggcttgatac aggaacacat
     7501 gaatcattgc ttgaagcatc tacctttatc gaaacaattg aaaaacgtca gaacttaaaa
     7561 gtggcttgtt tggaagaaat tgcttaccgt atgggctaca tcgataagga acaattgctg
     7621 gcccttgcac agccattgaa aaaaaatcaa tacggtcaat atttattgaa tttggccgct
     7681 gaataaggag tccttacaga tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt
     7741 agaaatggac gtctttggcg atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa
     7801 atttgcagaa catggattga aatatgactt tgttcaagac aaccattcac tatcagctga
     7861 agctggtgtc cttcgggggc ttcatttcca aaaaggagaa gctgcacaga caaaattgat
     7921 tcgggtagtc actggagcag ttttagatgt agtagtggat atccgcaaag gtagcccgac
     7981 ttatggtcaa tgggacggat ttatcttatc tgaacataat catcgacagt tgcttgtacc
     8041 taaaggatat gcccatggat tcgttacttt gacaccaaat gtgaacttcc tttataagtg
     8101 tgataattac tataatgcag aagcagatgg gggcatcgca tttgatgatc cagatttagc
     8161 gattgattgg ccaattgatc catcaagagc gattatgtcg gaaaaagatc accgtcatcc
     8221 gacattaaaa gaatttgaag cagaaaatcc atttgtttac ggagaaatct aagaattaag
     8281 gagaaatttt cttatgaaaa acatcattgt taccggaggt gccggattca tcggctctaa
     8341 tttcgttcat tacgtggtaa acaatcatcc agaagtgcat gtcactgttt tggataaact
     8401 gacttatgca ggaaacaaag agaacttggc tggacttcct agcgatcgcg tagaattggt
     8461 cgtaggagat gttgcagatg cagaattagt ggaccgtcta gtaaaagaag cggacgcagt
     8521 tgttcattac gcggcagaat cccataacga taattcatta aaagatcctt tcccatttgt
     8581 acaaacgaat atcattggta cgtatacact tttagaagct tgccgcaagt atgatgtacg
     8641 ctatcatcac gtatctactg acgaggttta cggcgatctt ccattacgtg aagacctgcc
     8701 aggacatggg gaaggcgaag gagaaaaatt cactgcggaa acaccatata atccttcaag
     8761 tccatattct tctacaaaag ctggttcaga tctgttagta aaagcatggg tgcgttcttt
     8821 tggcttacgt gcaaccattt caaactgttc aaacaactat ggtccttatc agcatattga
     8881 aaaatttatt cctcgccaag taacgaatat cttaagcggt attcgtccaa aattatatgg
     8941 cgaaggaaag aatgttcgtg actggatcca tacgaatgat cattcttcag cagtgtggct
     9001 gattttgacg aaaggcagaa ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga
     9061 taataagacc gtgatggaat tgatcttaga aatgatgggt caaccaaaag atgcgtacga
     9121 ccatgtaaac gaccgtgcag gacacgactt gcgttatgca atcgattcga caaaattacg
     9181 ggaagagtta ggatggaagc ctgagtttac gaacttccgt gatgggttgg ctgacacgat
     9241 caaatggtac gaagaacatg aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc
     9301 atatgccaaa aatggacaat aatacagaga aaatgtcact gtattaatgg aaaaacacat
     9361 gtgaggcagg cgctcgcctg tctctttttt aaaataaaaa tcaaggagcg gataatcata
     9421 tgtttttatt aactggagga aatgggcaac taggtacaga attgcgccat ttattagatg
     9481 aaaaaaatct gcaatatgtt tcaacagatg caaaagactt ggatatcaca gatgccgaga
     9541 aaacaatggc ttacatcaca gaattaaaac ctgatgttat tttccattgt gcagcttaca
     9601 cagcagtaga taaagcagaa gaagaagcaa aagaattaga tgagaagatc aatgttgatg
     9661 gtacaagaaa tgtagcactt gcagcaaaag ctgtgaatgc tcaattcgtc tatatcagta
     9721 cagactatgt tttcgatgga aaaaacaaaa atgtagaata cgaagtagac gaccaaacga
     9781 atccactaaa tgaatatggt cgtacgaaat tattaggcga aaaagcagta gaagagattt
     9841 tagaagacta ttatatcata cgaacttctt gggttttcgg gatctatgga cacaatttcg
     9901 tcttcacgat gcaacgccta gcggaaaccc gcgacaaact gactgtagta aatgaccagt
     9961 ttgggcgacc aacttggaca cgtactttgg cagaatttat ggtatatgtc atcgaagaaa
    10021 aagcaccatt tggtatttat catttgtcga acgaaaatag ctgttcttgg tatgaattcg
    10081 caaaagaaat attgaaagat acagatgtag aagttgcacc tgttacgtca gaagaatttc
    10141 cgcaaaaagc aacacgtccg cagtattcag tcatgagcct gaaaaagaca gaagagttag
    10201 gatttgtcat accaacttgg caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa
    10261 tgaataagta atttcctttt atcaaataga atttggagca tgatatggaa aaacaaacaa
    10321 gaagtgcacg acgaaaagaa aagccaaaag cgaaccaggc agattcgaat ttggtgcttg
    10381 acctgtttct aaaactccgc tatcttattg gcgttttagt catcgtggtc atcgttactt
    10441 ttaatttaaa cggaagttca cttggtgttt gggataaata tgtgtcgcaa agagacgatg
    10501 ggaaaaaaac tgatgtgatt tttggacaga atcgggaagt gcggtcagac gaatggctgg
    10561 tgcagacacc tttttacctc tcacaagctg aagaggactt tccgttggtg aacgaggatt
    10621 attcattaaa cggacaaaac atgatcattg cgtataattc tcctgtaaag gatattacgg
    10681 ttatcggaaa accattcaac tggggattct ttttcctcgg aagagatcga ggactttcct
    10741 tttattgggc gatgaaatta gtcgtcatgg tcttgcttgg attcgaagta ctgatgatat
    10801 taacgaagag aaaaaaagct ctatcgctaa tcggtgcatt tgcgctgaca ttttctccag
    10861 cagtccagtg gtggttcatg caacatgtgg gcgatttgat tttctttact ttgggactga
    10921 tgactgcttt ttatcattat ttctacaatc atgataaaaa atggctacga acgttgatga
    10981 tgcttttggt ggttattttc ggtattgggt ttattttagt gatttaccca gcccatcaag
    11041 tgatgctggc gtacttgctg attttttatt ttgttggcct gcttatttac tatagcaaga
    11101 aagtttcatg ggatctgttg gatgtagtgt tgattggagg ggcagtttta tttattggcg
    11161 gagtcatggt tcatttctgg ctaacgtcaa aagacgcatt gttagcttct ttgaatacct
    11221 tatatccagg aaaacgggta agtactggag gaaagtggac gattggcgaa tttttttctt
    11281 tccttacaaa ttggaaaatt ccttttgaag atatcaacta ttcgaataac tctgaagtgg
    11341 ccctgttcta tcactttttt ccaactgtat tcctagcaag tccatttgtg ttatttggca
    11401 aaaaagatag cgaacaaaag ttactgggac gtattttgat gattttttgt ctgttttcta
    11461 tcttttggat taccgttggg ttgccaaaat ggctagcaga aatcactctg ctgtcttatg
    11521 ttcctccatt aagagccatc cttactttta gttttgcggc ttgtttatta tcgatctggt
    11581 ttatcgctta tatttggaga gaagatgtta ttcctatttg gtataaattc attttgttgg
    11641 cagtaaatgc atggtcttat ttctatgcga ttacgcattc gaaaatggat aattattttt
    11701 cggattttga aaccatcgca gttgctgtgt tgggaacagt gattttagct tttgcattat
    11761 ttggctggag acgcttgttt tacctcgctt ttgccgcctt agttatcgta tctggctttt
    11821 tcgtcaatcc agtcgtagaa ggtacaggag cgatttttga aaaaacactg gcaaaagaaa
    11881 tccaaaaaat cgatcaacaa gatcctggta aagtttggtt gtctgaagat gaattatata
    11941 acttcacccc aacattggga gtgaaatcct tcaatacagt acgcttttat ccagatatag
    12001 ctgcatggaa gaaaatcgat ccgaaagaag aatacgagaa gtattataat cggtatgctc
    12061 atacaagagc ttttatcgct aaggaagata caaagttcat tttagatcgt ccagatatgt
    12121 tttcggtcac gttgaatttt gaagacgcag aaaaattagg gatcgactat gtgatcagta
    12181 aacgaccatt aactgattac aaccaattat atgaagcgca gttcacgcag ctatatggac
    12241 cggataaaga cggttatatg atttttaaag tagaatatcc ggattatcca gatgtaccac
    12301 aaacacaaac tcaggaaaat cagctagtcg tacagtaata ggctagctga gaataatcct
    12361 gaaatgttag tagaaaggag agtggagata attcaagcaa tcgtacaaaa atttgtcaac
    12421 aaaatgagtg tcggtttctt gcttttgtta ctgacgctag tcttttttgt ttggacagca
    12481 gggttaggtt ttccagcaac tttgtttata tataacaata gttcattact tatcgctttt
    12541 agtgtggcta ttttgctcat tttaaatgtt tataaagcaa aaggcgtcga ttttctttat
    12601 ctaggtgttg cgcttgccct tttcattttc ttttcttact ttcgggaatt aagaaatgag
    12661 tctttatttg gagacttact gtttcctttg attattttat tttgtttagt aattaaatgg
    12721 atcgagtgga caaaatggga tcgcggtatt tatctgattg tcttttcggt cttcctcgga
    12781 atcacgttat atcgtgtatt tacagagatc caagtaccag aaggacaaag catctgggct
    12841 ccaggcaatt cgctttctga tatttggatc aatacgaata taatcggaag ttctttgatg
    12901 actcttgctt tattgatcag tggatttgcc agttcttttg aacgatggta tgtacgaatc
    12961 atcggtgcag tagcagttgt tgcagctttt ctcgggattt gggtctgcca atctagaggt
    13021 gcattagtcg cgttgattgt ttttgcgata ttggatcttt tacctaaatc gttgatgcgt
    13081 gtcattcgtg cgccatttat tgcttacacg gttacgattc ttttagcttt gccgatttct
    13141 tatctggcgg ctgtctctga aaaagtcaat ttgtttaccg gtcgagaaga tatttggcat
    13201 aaattctatc agacgctagg agaaaaatct gaacaaattt tactaggaat gaaaacattt
    13261 atcttccaac gaggaaacca atttttagga aaccacaata gttataattc tattttgaat
    13321 atctatggat tgataggatt tgggattgct gctttgctgc ttatcctttt tattggtcgc
    13381 ttaacgttga aagcagattt atccaacggg cagatgactt ttatctgggc cttttttgcg
    13441 gtgatgatgc agtcatttat ggaggataca ttgacttcat acccatgggt tccgattatt
    13501 tatatcctgt tggctatggc cgttcatcga tatgatgaac cagaagttgc ggaggaattc
    13561 tatgttatgg aagaggcata tgacgatatg acttatgatg atacatctta tgaggaacaa
    13621 ccagatagcg ctcattcttc caggatccaa cgctatcatt gataggcaca aaatacgaga
    13681 gaggctgtga caaccgtcgc agcctttttt ggtttattgc aattgtctag catcttctgt
    13741 aaaatagaaa cgttagagaa aatcttgtat catagaaaat taagaaagaa ataagaacgc
    13801 agctcggtac caaaaatatg gtaaaatggc acttgactgt aattcactga aaatcgcttg
    13861 gaggaacaca aatgtttaaa gatttgtatt tatttatgaa ggacatttat cagaacagaa
    13921 aattattgct acagttttcg atcaatgact tcaaatcaag atatgcagga tcgtttttag
    13981 gaatcttatg ggcgtttatc aatccgctat ttactgtatt gatttattgg ctggtatttg
    14041 gtgttggact gaaagcacaa atgatcgatg caaaataccc atttatcgta tatttagtta
    14101 ccgggatgat tccctggttc ttcttctcag atgcatttgc atcaactacg gttgtttttc
    14161 gggaatatac ttatttagta aagaaagtcg tattcaatat tcgtatcttg cccacgacga
    14221 aaatcctatc caatctgtac acccatttat tcttcattct gattggcata gctgtcgcgc
    14281 ttataaatgg tatatatcct tcagcaatgt ctttacagct gatttattat ttattttgca
    14341 tgtgtgcttt cttgacagga ttgacttggc tgacggcatc gatccaacca tttttacctg
    14401 atatcatgca attaattacg atcgtgctgc agttgatcat gtggacatta ccgatattat
    14461 ggagccctaa tcagtttgat tcaggtcttg taagcatatt gaaattaaat ccgctgtact
    14521 atgttgttca aggttatcgt gagtcatttt taagtgaagc ttggttctgg gagcatggaa
    14581 catatacttt gtatttctgg gttttcacgt tagtaatgtt cgtaatcggc tctgcagtgt
    14641 tccgtagatt gaaaccacat ttttcagatg tgctgtaagg agaatagcta tgacagaata
    14701 tgcaattaga ctaaaagata tcacaaaatc ttataatatg tacgcaaaac cttcggatcg
    14761 atttcgtgaa gccctcaatc cttttaagaa atcctatcat gatgtatttt atgcgttgaa
    14821 aaacgtgaat gtagaaataa aaaaaggtga gatgatcggt ttcgtgggag aaaacggatc
    14881 gggaaaatct acaatgttga aaatcattac gggagtactg acaccaacat ccggcacgat
    14941 ggaaatcgaa ggaaaaatct cagctttgct agaactagga tcaggattca atcctgagta
    15001 ttctggctat gaaaatatct atttaaatgg aatggttcta ggattttcaa gagaagaagt
    15061 cgatgaaatg gtcgatgata tcattgaatt tgctgacatc ggagaccata tttatcaacc
    15121 tgtaaaaact tattcaagcg ggatgttcgt tcgtctagct tttgctgtag caatcaatgt
    15181 gaatcccgat attttgattg tggatgaagc attggcagta ggcgatcttg aatttcaact
    15241 aaaatgtatg gaaaaattta cagaaatcaa gaactcggga aagacgattt tattcgtttc
    15301 tcatgatatc agttcgattc gtcgtttttg tgatcgttcc tattggttga aaaacggaga
    15361 agtcgtcgaa tatggcgata cgatggaagt aacgacgaat tatgaaaact ttttaaagaa
    15421 aaaatcagtt aaaacagtgg atcggaataa aaaccaagta gaacggttcg ccgctccaga
    15481 tatcgtcgac gtgacgaaag cggaattgtt ggataaagac aaaaatccgg tggatatgct
    15541 ggaacaagga gaaaaactat acgttaaagt aacttatgaa gtaaaagatg atacaatcaa
    15601 aaatccggtt ttaggaattg cgctacgaac agtcgacaat ttttatgtat gcggacttaa
    15661 tactctacta gatggtatca agattccttg gaaaaaagga gagaatatct tttatctgga
    15721 gtatgaaaag ttaggtttgt tagctggtga atattatttt gacgttgcgg tctttgaaga
    15781 aaatgcaacg gttcctttgg tatataagac aaagtacatg aacttgtttg tcagcggttc
    15841 ttatatcgga gaaggcatcg tggtcttaga ccataaatgg gaagaaggga ctcatagcaa
    15901 tgaaatatga ttttgaaatg gacctagatg aacaaagcag tgtgggaaaa attgccgcac
    15961 agatcaaacc aggaagtaaa gttttagagt ttggtcccgg aaacggccga ttaacgaaac
    16021 atttaatcgg agcaaaacaa tgtgaagtga gcattgttga gttagataag gaattgttcg
    16081 attttgtcag cgaatttgct caagatggat tttatggtga tatcgaaagt tttgagtggg
    16141 caaactatta tgctggacag acatttgatt acgtcttgtt tgcggatgtt ttagaacatt
    16201 tggtagatcc tggaaaaaca ttgaagaaag tcagagaatt tttgaatgaa gaaggagaga
    16261 tcctgattac attccctaac ttggcgcata attctgtcat gatcgatttg ttcaataacc
    16321 aattgccgtg ggcttcttat ggtctcctag atgaaaccca taattctttt tatactcatg
    16381 atggcttcca aaaagttttt gaaaaagcgg gattattcat taatattgaa gattatttgt
    16441 acttagctgt aggtgataca gaattaaaat caacttatga agaattgccg gaagctgtac
    16501 gctatgattt caaaatgcga ccatttggtg aagtctacca atactttttc tcattgatga
    16561 aacatccagt agcacaaagt tccattgcag agccacaaaa ttctaattat gtaaaagtgt
    16621 tagaagtaac ccagcaaacg aaacaagatg aaacaatcca acaaatacca ttcaataatt
    16681 tcacagggga aaatgagaca ctaagtttcc ccgtatcaga aacaacagaa agaatggtgt
    16741 ttcgcttcgc gcaacaacct agctttatcg aattcagcgc agaagctgcc ggagaaaaac
    16801 taacatttat cgattcgaat gctgtagtca aaacagtaaa tgattgttat ttgtttgatg
    16861 ggaaagaatt accagagttc gttctaacgg atatttctgg gaaagaagtg actatccatt
    16921 gtcactatcg ctttatcggg gaattgacac caacaatgaa agaattgttg gaaacgattc
    16981 gtcccatggc tgaagtcaca aagcagcttt ctgagaaaaa tgacgaatta gaaagagaaa
    17041 atcatcatct tcttgaggaa aatacaagac tagaccatac gttaaaaaca accacaaatc
    17101 gctattgcac attgttggaa agtgatgaat ggacaataaa acatagacta ggacgacgta
    17161 agaaagaaac ttcgaaaaaa atccaagaaa aagaactttc tatatgtatt gacgagaaaa
    17221 tctgggatgc agaaacaaaa atattaaaaa tcatcggatg gggaatcgct aattcggatc
    17281 gccagccctt gtcctataag ttatcagctg accaatcgcc atttttcgaa gcaattcctg
    17341 tttcacgaga agaagtcaat caagcagaac agctcgcaaa aggaacagaa gcgggatttg
    17401 agttacgaat cctttgcgag caagaacgtt cattcttagt ggaagcagta gctcaaaatg
    17461 gccaatcatg gatcattgaa atgtaaataa tgattaactt ataaagataa ggagtaaacg
    17521 aatcgtgcaa aaaaaagaaa ttgccgtatt tatcgatcaa atcactcgtg aacgggccac
    17581 tggcgatttg cttattgtgg gctgggcaat tgatgaagtg accaaagaaa tccctacgat
    17641 aaaggtagaa aaagaaaatg tgattgctga agcaacacat gtcgtaagat tggatatcaa
    17701 tcatttatat aatcttgatg ttaagaccca aagcggtttt aaggtacgtt tatcagggaa
    17761 aatgagagga aaagccatct tagacttcca aacagcgaga catcaaaatg gcatagctgt
    17821 gaaattaaac ggtaaatacc cttatgatga cggcattgaa tcttcatggg aaagaaaaaa
    17881 aagattactg aaaaaaggga taaattatgc aaggactcat ggcgtaaaaa aagcgatccg
    17941 tcgggtcaaa ttggaattga agccaggatc gatcgactat gcagagtgga tcagtcgtca
    18001 tgagaaacca gatttgaaag agcaaaggaa agaagcacaa ggatttgcgt accgtccatt
    18061 gatttccata cttatgcctg tctataacgt tgaaatcaaa tggctcgaaa aatgcattga
    18121 ttcggtactg gatcaaacgt atgatcattg ggaactatgt atcagtgatg atgcttctac
    18181 tgatccagct atccgaaagt gtctggaatc ctatcaagca aaagatgata gaatcaaagt
    18241 cgttttccga aaagaaaatg gacacatcag tctggcaact aattctgcat tggaaatagc
    18301 agaaggagaa tttatagcac tcttagataa tgatgatgaa ttaccgccat ttgcattata
    18361 tgaagtggca aaagtgctaa acgtacatcc agaactggat gtgatctaca gtgacgaaga
    18421 taaaatcgat gcagatggca atcgctttga tccgcatttc aaagcagact ggtcgcctga
    18481 tacattgatg ggaaataatt atatctctca cttaggcgta tatcgaacaa gcattgttaa
    18541 agaacttggc ggtttccgta aaggctatga aggttctcaa gattatgatt tggtccttcg
    18601 tgtgacagaa caaatccctg cagatcatat ttaccacatt gaccgagtct tgtatcattg
    18661 gcggacgatc ccaggatcaa ctgcaagtaa tggcgaagcc aaaagttata tctatgattc
    18721 cggagtgaaa gcattgacag atgccctgtc tagaagaaac atcaaaggaa gcgtacgtcc
    18781 tggcagaatt tctggatttt atgagattgc ttatgacgtc ttgcaagagg atttggtcag
    18841 tgtgatcatt ccaacgaaga atggctatga agatctaaaa acttgtgtgg attcgatcat
    18901 cgaaaaaacg tcttattcaa attatgaaat cattattgcg gataacggaa gtacagatcc
    18961 aaaaatgcaa gaattatttg cagaatacaa gcatcagctg aaagaccgct ttatcgtaga
    19021 attgattgac attccattca attattctcg aattaacaat ttagcagcag aaaaagcaaa
    19081 cggcaaatac tttttattct tgaataacga tacagaagtg attgagccag attggatgac
    19141 ggtgatggtt tcttatgcac agtttgatcg aatcggctgt gtaggagcaa aactgtttta
    19201 tccagatgat acaacgcagc atgcaggagt attattagga atcggtggtg ttgcagggca
    19261 tgcattgaat aactatgatc gaacacattg tggttatttc ggccgattgg tcattgacgt
    19321 caattactta gcagtcactg cagcttgtat gatggtcaag gcagctgact tcaatgctgt
    19381 taacgggttt gatgaaacat tagaagtagc attcaacgat gttgacttgt gcttgaaagt
    19441 ttatgaactg ggtcgttaca atgtctatgc acatcaagca gaattgtacc atttcgaatc
    19501 taaatcaaga ggttatgaag atactccaga aaaacaaaga cgttttgctg gtgaaatcaa
    19561 aaaaatgcaa gataaatggc cagcatatat tgcgcatgat ccattttaca atgataattt
    19621 aacaaaagaa ggaatcggcg acttttcgtt gagaccagat taaactataa taaaaagctg
    19681 ttatgttgtg attcgctcgc acataacggc tttttatagt tttttgtcaa aaaaaggcag
    19741 aaggtgccga ctcaaaaaaa taagtcgagg ttgggacaga agtgtttaac tccgagaaat
    19801 aagaagaaat tcacgaaaat tgcttttcaa atttttgtga atttcggctt atttaagaag
    19861 aagttgcttc tgctcccacc gtttattcgt ttttaaagaa tgagacaaaa gtgtttctta
    19921 cttttgtccc acgctcttat tcatgctcag agcgccaata gatacaaaat catagccaaa
    19981 gtttcacgaa ccctagctgt caaagaatcg atattttatt ttaatatagg ctattttaaa
    20041 ataaatggtt taaggtattc ctctacaaaa gccactctat caattatcat gggtttaaaa
    20101 taagaagtga tggagataac cagattgttc ttagcgtcaa tataaagaac atttccacta
    20161 ttaccaattg ctgaatatat ttcgttctca gaatctaata cccaccataa atagccatat
    20221 ctcatatttt gtaagtcttc taaagcactg gttctaggtt tgaacatctc gtttatccac
    20281 atgggtggta cgatttgttt tcctttatac atccctttat ttaggcataa tacaccaatt
    20341 tttgccagat cacttgccga taaacacaaa ccatatcctg ccgtcccgat atttagtgga
    20401 tcacaaaacc agacattggt tttaggagac ttatccattg taaactgctt atgttcttca
    20461 gctgtttcag cataataatt gacatggttt ggtatcccta taggatcaaa tagatactta
    20521 ttggcaaaat caacagttgt tagcttactt gtctgataca acaagccagt taatatgtgt
    20581 aaacagacag tttgatagtt gaattcgccg gttatacctt ttcttcctcc caataaatct
    20641 agtgatgttt ttgtccaatc ttcacttgaa cagacttttg tccatggatc gcctttacat
    20701 ttgtagggtg ctttcattgt tagcaagtgc ttgattgtaa tagcgtaaat agttttctcc
    20761 ccgcgtttca ctttatactc tggaaaataa tctaatactt tatcatcgat acttttgatt
    20821 aatccttgct ctaaagcaat gccaatcagc aaagatacaa tacttttcgt tgctgacatg
    20881 atatgataag tatcgtcttt tttataacca ttccattcat ctgtataaac ttctacccca
    20941 tccttatatg cgacaatttg acatatattt ggttgtcgaa aaccaataaa atcatgcaac
    21001 tcttgtttat tcataagata ttacctcact ttcttctata agcataatgt cattaaaaat
    21061 cgatttcaat tttttcgatt cttcacgtaa tacaggtatt cttatggact agacagcgct
    21121 ttgggaagat ggattctctg aaaaatataa aggttcactt tatgagtgga taaaaaaagc
    21181 agcaaatact tcgacttctt taatttacca acattagtga aaatcgattc atctggagat
    21241 ttgtaattta gagtccttgt cttgattaga ggtgtataaa gctttttatt agacattttt
    21301 aaattccatt acaatttgct tataaactag gtttttaaag gtgactatgg taagataata
    21361 gagatattat tttgtgatta ggagtgtata tatgaataaa aatggagaat ggagtgctgc
    21421 acgacggata ttcattatat taatggatgt tattgtatac aatgcagcaa tatatagtag
    21481 tttcttactg aagtttcgtg gagagatccc attacgaaat ttagaaactt tccaacactc
    21541 agcaattatt atttcagtga tttttattgc gttgaacatt ttgttgggtg catatgtttt
    21601 ttataatcga atgatcagtg acatcgtgtt tgtcacagtc atcggtcagg tattgatgac
    21661 actagggatc atggtagtga cttttgcggg ccgctggttt gcttttccac gtcttgttat
    21721 cttactttca tttgttctcg gtacgctttt cttaagtatc tatcgtattc tcgtatataa
    21781 actttattta cgcgtaagtc gagataaaaa ggtcgtcatt attgggttag aaaaggatgt
    21841 cgcaccagca atccaaaatt ttggttcaaa gaaaaacaac aaacataaag tcgaagcagt
    21901 cattcttgat aactattatg caaacatcaa aaatatgatt aaagacatcg atattgtcta
    21961 tcttgcttct catatcgaag aaactgaaaa aatcaaaatc tatcaattag ttactaaaaa
    22021 tgaaaaaaaa ttgtttttga atacaacatt tgaaaacttg acattgatca atccaaatat
    22081 tatgaatttc gaagatgaaa gtattattga agcatctgga tttagaatcc aacctgaata
    22141 tgaactattc aaacgtttgt ttgatatcat cgtttcactt gttttattag ttattgcttc
    22201 accatttatg ttattcacag caattttagt caaagtgaca tcaccgggac caatcatcta
    22261 taaacaagtg cggatcacaa aaaatcaaaa ggaattttca atctataaat tccgctcgat
    22321 gaccgccaca gccgaagcaa aatctggacc agttttagct aagagcaatg atgcacgagt
    22381 aacgcctgta ggtaaattta ttcgagctgt ccgctttgat gaattaccac aaatcttcaa
    22441 tgtgctgaaa ggcgatatgt cgatcgtagg accacgtcct gaacgtcctt tcttcgtcga
    22501 tcagttcaat gcagaaaacc cttattacta cttacggcat aatgttcgtg ctggtatcac
    22561 tgggtatgca caagtctatg gaaaatatgt ctctgattac aacagtaaat tacgttttga
    22621 tttactttat atcaaaaaat attcattgat gcttgattta aagatcttgc tgcaaaccat
    22681 caagatccta tttgataaaa tgtcctctca aggacttgaa gaagacgaaa atgtgatctc
    22741 attggcagaa attgcttttc cgaatgaaaa aatctttcgc tagttgggag gcgtaaaaat
    22801 gaacaatccg aaattcagca taatcgtacc agtatacaat gcagaagcaa ctattggtcg
    22861 aacgattgat attctaaaag aattggatta tgaagagtac gaagtcgttt tagtaaatga
    22921 tggatcaaca gatcaaacac aggaaatcat tcaagaactg attgaaaatg atgaacgttt
    22981 tgaattgatt actacgacca atcaagggcc cggattagca agaaacgaag gaataagaca
    23041 gtcaaaagga gaatatctct tattcttcga tgcagatgac acgccagtaa aaacgatctt
    23101 gtcagattat gatcatttgc tagaaaagag tccaaaagcg gatcttgttg ttagttcgtt
    23161 tgtttttcgt acaatggacg agggaagaat cgtttccgaa aaagaaaact tagttacaga
    23221 atgtcactat ctaactcacg aagagtttat aaaagacatg tatcagttaa tgaatgaaca
    23281 gttgatgtat gttgtatgga acaaatgcta tcgtagcgat attctgaaaa ataaccaaat
    23341 tctatttaaa acttataata gttgtgagga tcgaatattt aacttacatt attatgaata
    23401 ctgtcatcaa gtgatcatga acccaaaagt agaatatatt tatgaatttg aaggtgggaa
    23461 ggggatcact aatcaatacc gacctaacaa gttcgaaaca tttaaagaat tctattcatt
    23521 agcaaatact gtaacagacg atattaataa agacggtatg gcggcgcttc ttttaaaagg
    23581 gacaacttca gttatttttt ctatttatag tacctctgca agaacttcaa aagagaagaa
    23641 agacgaagca aagcagatat tgagtgatcc agtgatccga gaagccaagc aaattgctaa
    23701 gacggattct tcagcaaaaa aaataacaaa gttgctttac aatatgccag ctccactatt
    23761 tttatctgct gtaaaagttg gaagcttcgt tgaagtgaaa atgccagggt taatggctgc
    23821 tttgaagcga gtatattagt taatggagga aaatataatg aaaagaattt tgcattttca
    23881 gggacgaatg ggtttaggtg gagcagagtc tttcatgatg aatttatatc gtaaaattga
    23941 tcgtacaaac tatcaatttg attttttgat ttatgaagat tatgcagatg ttcaagacta
    24001 tcattcagag attgaaagat taggaggccg tatttttgtt gtacctaatc ctaagaaaaa
    24061 tatttttaaa tatttaatag aagtgaataa gttactaaaa aaagaatctt tttctatagt
    24121 tcacaatcag gtttattttg gcgggggaat taatttatgg ttagccaaaa agaatggaat
    24181 cagacagaga attgctcata gccatgcaac tgaagatgga aaatctcaaa atattgtaat
    24241 gaatgtattg cgtaaatttt taaccaaatt gttgttacaa aatgcaacag actaccttgc
    24301 cgttagtcaa gaagctggtg aaagtctgtt tcaaaaccat ccctttgaga tagttcataa
    24361 tggtattgat ttagagcttt atagtaaaaa ttcagaagct aaggttaata agagaaaaga
    24421 attggatatc tcaatgagta cttttgttgt gggaaacatt ggacgattag agaaacaaaa
    24481 aaatcaatct tatttgttag agattttttt agaaatacta aaaatgaatt ctgattctta
    24541 cttgatttta gtaggtgaag ggtcacttag aaataaactg aaagccaaag cagtagaatt
    24601 ggatatagag aaaagagtaa aatttttagg agaaagaaac gatattcccg agttattatc
    24661 tgtgatggat gtttttgtaa tgccttctct ttatgaaggt ctgccaatgg ttggattaga
    24721 agcacaagca gcaaaattaa aattagtctt agcagatact atttccaaag atacaaagtt
    24781 aacaaataat gtggattttg tatcattaga tgaaacacca caagaatggg caaaaaaagt
    24841 aataaaagag ccgtatgcta ataaaataac acaagaacta aagagttatg atgtttcgta
    24901 cactactgaa caaatggaga gtatatatag tagagatcat taaaaatgtt caagatattt
    24961 taggagtgat tatttttgaa aatatgttat ttgatattgg catacaatga tattggcaac
    25021 ttaaagagat tggtcaatcg attgaacgaa aatgcagttt ttattattca ta
//
LOCUS       DAJSMI010000022        24196 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJSMI010000022 DAJSMI010000000
VERSION     DAJSMI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.022"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            257..1168
                     /locus_tag="ON430_000758"
     CDS             257..1168
                     /locus_tag="ON430_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3218701.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1424..2341)
                     /locus_tag="ON430_000759"
     CDS             complement(1424..2341)
                     /locus_tag="ON430_000759"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCR3218702.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2543..2980
                     /gene="zapA"
                     /locus_tag="ON430_000760"
     CDS             2543..2980
                     /gene="zapA"
                     /locus_tag="ON430_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR3218703.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            3038..3586
                     /locus_tag="ON430_000761"
     CDS             3038..3586
                     /locus_tag="ON430_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCR3218704.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            3701..6061
                     /locus_tag="ON430_000762"
     CDS             3701..6061
                     /locus_tag="ON430_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR3218705.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6148..6462
                     /gene="trxA"
                     /locus_tag="ON430_000763"
     CDS             6148..6462
                     /gene="trxA"
                     /locus_tag="ON430_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCR3218706.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6644..8422
                     /gene="uvrC"
                     /locus_tag="ON430_000764"
     CDS             6644..8422
                     /gene="uvrC"
                     /locus_tag="ON430_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR3218707.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8567..9466
                     /locus_tag="ON430_000765"
     CDS             8567..9466
                     /locus_tag="ON430_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218708.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9459..10721
                     /locus_tag="ON430_000766"
     CDS             9459..10721
                     /locus_tag="ON430_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218709.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            10884..12782
                     /locus_tag="ON430_000767"
     CDS             10884..12782
                     /locus_tag="ON430_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCR3218710.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(13081..13800)
                     /locus_tag="ON430_000768"
     CDS             complement(13081..13800)
                     /locus_tag="ON430_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCR3218711.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            14137..14469
                     /locus_tag="ON430_000769"
     CDS             14137..14469
                     /locus_tag="ON430_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218712.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14456..16459
                     /locus_tag="ON430_000770"
     CDS             14456..16459
                     /locus_tag="ON430_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCR3218713.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16478..16948
                     /locus_tag="ON430_000771"
     CDS             16478..16948
                     /locus_tag="ON430_000771"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCR3218714.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            16969..17553
                     /locus_tag="ON430_000772"
     CDS             16969..17553
                     /locus_tag="ON430_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCR3218715.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17568..18554
                     /locus_tag="ON430_000773"
     CDS             17568..18554
                     /locus_tag="ON430_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCR3218716.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            18544..18855
                     /locus_tag="ON430_000774"
     CDS             18544..18855
                     /locus_tag="ON430_000774"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCR3218717.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            18877..20658
                     /locus_tag="ON430_000775"
     CDS             18877..20658
                     /locus_tag="ON430_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCR3218718.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20651..22027
                     /locus_tag="ON430_000776"
     CDS             20651..22027
                     /locus_tag="ON430_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCR3218719.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            22034..22669
                     /locus_tag="ON430_000777"
     CDS             22034..22669
                     /locus_tag="ON430_000777"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCR3218720.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22699..24054
                     /locus_tag="ON430_000778"
     CDS             22699..24054
                     /locus_tag="ON430_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCR3218721.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT         7965 a         4163 c         5298 g         6770 t
ORIGIN      
        1 tgtcggtgtt ccaggaagtg ttttaattta ataacgaaga aattaattgt ttgtagaatg
       61 aaggaaacta tttattgggt agatgaatca ccacgaatag tcaaaaggag atttcaggat
      121 atgccaaaaa tgaaaaaaat cattttagca gtaatgttat tttctacggt tatatctttt
      181 acagcccctt ttttgcgtac ttaagctgat tttaagtttt gacccttagt ctggatctac
      241 gaggtgagag tcaagaatga aaaaaataat tggagctttt gtatttgcaa tcattatcat
      301 tattatctgt tttcaaatag atgtttttgg tcaaaaaaag atagttggac aaacggtcag
      361 cagtacgaac agtacggtta caaagacaaa ggaatcattt gatgatacag aacaagagac
      421 attaatcaaa atcggaaaaa atgaccgtac actatataca gatgactcgc taactacccc
      481 acttgcaact ataaacggcg gagagctggc ggagtttctt tctgaaacaa aaaatgctta
      541 tcagatcaag acaaatgatg gctatactgg atatttagcc ctcgaagatg gaaccaaagt
      601 cacaaagaat atacaaacaa aaccggaaga gctatctgat gcagtgattg tattagatcc
      661 tgggcatggc ggaaatgata ctggggcatt aagcaatgat gaacaaacag aagaaaaaga
      721 tataacatta agtacagcca taaaagtaaa aaaggcgcta gaggatgctg gagcaacggt
      781 ttatttgacc catactacag atgaattagt acagcttgga gatatatgcg actacagtga
      841 agaaaaaaag gcagatgtct ttatcagttt acacgcagac tctacggaat atgcgaatga
      901 agcaactgga atcacgacat attattacta tggacaagag gaaacattag cacagacaat
      961 tgctgactcc ttcactgatc tgcctctgga ttcaagagga atctccactg ggaactatca
     1021 agttcttcga gaaaatcttc aaccttctat tttaatcgag atgggttata tgaataatga
     1081 tagtgattta aaggaattag taacagacag ttatcagcag cagattgctg caagtttgac
     1141 acaagcattg accacttatt ttcagtaata aataagccca gccaaacaaa aatagctcct
     1201 catatttttc gtttggccgg gcttattatc gcagatgcaa gcttttgaac accgtttaag
     1261 gaagaggttg tgagcctgcc gtttaactcc gcgtcacaag gagcaatttc aaaaaacagc
     1321 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcatgag aacaccgttt
     1381 attcggaaca agggactgtg acatgacttt tgtcacagtt ccattatttt tttagcagct
     1441 tcaaagcttt ttctgtattg gcaaaatgaa gtttggcata tttttcaagc ataggcatgc
     1501 cgcctttctc caatagtttt gccgctactt ggtcagattt cgttccagct cctgatggaa
     1561 gggtgattcc agcttcagct gatgctttgt gtaactcttc caaaaagctg gcacgactga
     1621 tgatagaagc agcagcaaca gctaaatgat attgttctcc tttagtaacg aagtacagtt
     1681 tttcttgtaa ttgatttttt tctaaacgcg catatttttt aaaatttgct tccggcgtaa
     1741 actgatcgat caaaatcgct tctggttttt ctggctcgat tttttttagc agcaaatgga
     1801 tcgcttgatt gtgcaaagcg actttcatcc gaacggcatt gtattttggt tggatctcat
     1861 tgtatttttc tggagataag acaagcagtt tataggggat ggtttttttt aagatgtcag
     1921 caagctggat gatttggcta tcttttaact cttttgaatc tctaacacca agtttacgta
     1981 attctgcaag ctgatcacgt ttcacataag cagcacatac tgttagcgga ccaaaatagc
     2041 tgccatttcc gacctcatca ctacctaaaa cggataattg actgatattt tttggcaaag
     2101 aagaggctgt ttttgctggc ttggcttgtt tatcctgtgc ctccccccat ttatcagctt
     2161 ctattgctgc attcgtacct tgaaacatca cttttccgga tgtataagcg gtaatggtcg
     2221 tcccgtcttt ttttgcagca aagctagtat aaggaatctg tttatttaac aaataagagc
     2281 gatagtatgc tttcatttga ctaagttggt cgtttgttgc tttgataatg acgttgctca
     2341 taaaattctc ctcgttttta tacataaaac tataccgcaa ctcacgactt ttcgttttct
     2401 aagagattgc gagacatagc atttttttaa agtttatcat ataatagtta agtatgtgaa
     2461 aaatatttgt ggagaacggt gaaagaacca acttcatggt acactaaata aaactttcat
     2521 caaataggag gaaatgattc gtatggcgca cgaaaaaaca cgctacaaag cagtgattgc
     2581 caaccaaaca tatactatta tcggacggga aaccaagcat catatggata tcgtaacgaa
     2641 gctgatcaat gaacagttag cagaattgaa acaattatca ccacaaatgg ataatgaaca
     2701 agcagcaatc ttgatggctg tcaatgcgct ttcggatcaa ttgaagaaac aagaaagaat
     2761 cttggagctg gaagaagaaa cagcagaatt aaagaaaaaa atgatcaaat ttacagagct
     2821 tgaaaatcgt gtgaaacgca ttgaagcaat cgagaacgaa gctcgggaag tattgaaaga
     2881 aaatggtcaa gcagatcata ccattaaaaa tcatgtggaa gcacaacaga ttttaaacga
     2941 gaaacgaaaa ggacaaatta aacaaaaatc ttctaattaa gatcctttta tcaaaataag
     3001 aaaaatttaa aaaagttaga agagaaagga acgtagcatg ctgagtttgc tgattttatt
     3061 tttattgcta attgcgtttt tttctggagc acgtcgcggg tttgctttgc aaatcatatt
     3121 cgcgattgga tatgtattgt catttattgc tgcccagcat ttttacaaac cattagcaag
     3181 tcatttggaa ttatacattc catacccagc tgtcacccca acatcaaagt tagcattttt
     3241 tgatcaggta tttgcttttc acctagatga agctttttat gcaggaactg cttttctgat
     3301 tattttgcta attggctggc tgcttacccg atttgtgggg gtttttgtcc atggattgac
     3361 gtatgtgccg atcttgagac aggtagactg gattgcagga ggcatcctgt cactaatcat
     3421 ggcttacgtt actatatttt tgattttgca gttacttgcc tttgttccat tagatgtggt
     3481 ccaaaatcaa ttcggcgagc atagtttcgc tcgttttatc gttgagagaa cgccatttct
     3541 taccaacaag atgtatgagc tttgggtaac gaatattttg aattaaggaa aatagtgctg
     3601 gaaaatattt cgttttttgt acagagtagt ttatttaaga aggctgcgta gcgtatgtcc
     3661 ggcacagctc ttttttcata gtgaaagata aggtgataaa atgaataacc gaatccttga
     3721 aacattggaa tttgaaaaag tcaaacagat ggtcagacaa tttgtagtga ctgcacaagg
     3781 aaaagaagaa ctggcagaat tggtaccagt gagtgagaaa caaacgatca caaactggct
     3841 tcaagaaact gaggatggat tgaaagtgca acgtctgcga ggcgggatcc ctatacctaa
     3901 acttgaaaat atccgtcccc atatgaaacg gatcgagatc ggagcagact tgaatggagt
     3961 ggagcttgca caagtttcca gagtattgtc tacgacaagc gaattgaatc gtttcattga
     4021 tgatctttct gacagtgaaa tcgaatttgc acgtttgtat atgtgggcag atcagttagt
     4081 cacgatccct gttttgagcc gccgtttaaa ggaagcaatc gatgaagacg gacgcgtgac
     4141 tgatgacgct tctcctgagt taaagagcat aagacagaac attcggcgaa gtgaacaagc
     4201 tgttcgtgaa cagttagatg ggatcgtccg tgggaaaaat gcaaagtatt taagtgatgc
     4261 gatcatcacc atgcgaaatg accgctacgt tatcccagtc aaacaagaat accgcggtgt
     4321 atttggcggt gtagtccacg atcaaagtgc ttcaggacag actttattta ttgaaccaaa
     4381 acaagtggtg gatttgaata atcgtttgcg tcaataccaa atcgctgaac gaaatgagat
     4441 tcaacgtatt ttaagtgaac tttctgctga acttgttccc catcgtcaag aaatcatcca
     4501 caatgcttat gtgattggta aaatggatct gatgaatgca aaagcacgat ttggtaaaga
     4561 agtaaaagcc atcgtgccgg gaatcagtga agacaatcat gtcgtcttga aacaagcaag
     4621 acatccactg atcgatcagg aaaaagtagt ttctaatgac atcacaatcg gaaaagacta
     4681 tcaagccatc gtcattacag gacctaacac aggtgggaaa acgatcacgt tgaagacttt
     4741 aggtctgtta caactgatgg ggcaagcagg attaccgatt cctgcaggtg aagaaagtca
     4801 aatcggtatt tttgaggaag tatttgcgga tataggagac gaacagtcga ttgagcaaag
     4861 tctatcgacc ttttcttctc atatgacgaa cactgtagat attttatcaa aagtcaatga
     4921 aaaaagtctg gtattatttg acgagctcgg tgcaggaaca gatccgcaag aaggtgctgc
     4981 tctagcaatc gcaatcttag atgatcttgg taaaaagtct gcttatgtga tggcaacaac
     5041 gcattatcct gaattgaaag tttatggata caatcgggca aatacgatta atgccagcat
     5101 ggagttcgat gtggatacac taagtccaac gtatcgtttg ctgattggcg ttcctggacg
     5161 aagcaatgcg tttgaaatct ctagccgatt aggtcttgat acagaagtta ttgatgaagc
     5221 gaagcagttg atgaatgatg agagccaaga tttaaacgaa atgatcacag atttagagaa
     5281 tcgccggaaa atggcagaga ccgaatatct ggaaatgcgt cattttgttt ctgaggcaca
     5341 agaacttcac gatgatttga aagaagctta cagctatttc tttgaagaac gtgaaaaaga
     5401 aatggaaaaa gcaaagaaaa aagcaaacga agtcgtgtca gaggcggaag aaaaagcaga
     5461 aaaaatcatt gctgatattc gtaaaatgca acagcagatt gggcaaggaa atgtgaaaga
     5521 gcatcagttg atcgatgcaa agacccaatt agcaaatcta catcaagaag aaactttgaa
     5581 gaaaaataaa gtattgaaga aggcgaaaga acaaaaaacg ttgaaaccgg gagacgaagt
     5641 gttggttacg acttacggcc aaagagggac gcttttacgt aaaaacggga atcagtggca
     5701 agtagaaatc ggtatattaa aaatgaatgt gtccgaagac gagctgacgc ctgttgctcc
     5761 tcaaaaagag ccgacacaac gagttatcca tgctgtacga tctgaatcta gtagtcacgt
     5821 gccgaatcaa ttggaccttc gaggaaaacg atacgaagaa gcgctaagtg aggtagatca
     5881 atatttggat tctgccattc tagctggtta tcctcaagtg actattgtcc acggaaaagg
     5941 aacaggcgca cttcgcaagg gaattactga ttatcttaaa aaccatcgga gcgtcaaaag
     6001 ctttgagttt gcaccggcaa accaaggcgg aaatggtgcg acaattgtta aatttaagta
     6061 aagcttacta aaggtaagca gatggttcgc tatccaagta tagaaatgct atactaatca
     6121 tagattttaa aaatggaggt ctttgatatg tcacaagtaa ttacagataa gacatttaat
     6181 gaagaaacag ataaaggact tgtattgatt gatttttggg caacttggtg tggtccatgt
     6241 cgtatgcaag caccgatttt ggatcagttg gaacaagaat atgatgaaga agaattcaga
     6301 attgctaaaa tggatgtgga tgaaaatcca gaaactcctc aacaattcgg tatcatgagc
     6361 attccaacat taatgttgaa aaaagatggt caagtagtag aaaaagctgt tggtgttcat
     6421 tcaaaagaac aactacgtca aatgatcgat caatatttat aaaaacttac agcaaaaata
     6481 agctgagggt gggactgaat ccagttccgt cctcggtttt tttgcggttg agttaaggtt
     6541 attcaaatta atattttttg agtataatta cgttgtcagg ctggattgtt tttttgacag
     6601 acatgctata ataactttaa ttgaatatga aggggagtac cagatgaacg aacgaatcaa
     6661 aaacaaatta gctttacttc ctgatcagcc cggctgttac ttgatgaaag ataaaaatgg
     6721 aacgatcatt tatgtcggca aagccaaaat attaaaaaat cgtgtccgct catattttac
     6781 tggcagccat aatacgaaaa cggaacggct tgttagtgaa atcgaagact ttgaatacat
     6841 cgttacagaa tcgaatatcg aagcattgct cttagagatc aatctgataa agaaaaatga
     6901 tcctaaatac aacattatgt taaaggatga taaaacctat cctttcctta agataacgaa
     6961 tgaaaaatat ccccgtttgg tgattactcg taaagtacta aaagacaaag cactgtattt
     7021 tggaccatat cccgatgtcg gtgcggcgaa tgagacaaag aagatcctcg atcgattatt
     7081 tcctctgaga aaatgcaaac ctagtcagac gaaggagcct tgtctttact atcatttggg
     7141 acaatgtctg tgtccttatt atttcgacgt agatccgaaa gtctatacga aaatcgttga
     7201 tgaagtgaaa aagtttttaa acggaggaca cgaaaaaatc gagtcagaga tccatcagaa
     7261 aatggaaaaa gctgctgaaa acatggagtt tgaaaaagct gcagaatacc gagaccagat
     7321 ccgtgccatt gagacaatca tgactcgcca aaaaatgaca aatactgatt tactggatcg
     7381 agatgtattt ggttatgcga ttgacaaagg atggatgtgt gtccaagtgt ttttcgtaag
     7441 acaaggaaag ctaattgaac gagatgtttc aatgtttcct ttctataacg aagcagaaga
     7501 agatttcttg acttacctcg gtcagtttta tcaggaaaat gagcatttca ttcctaaaga
     7561 agtcttgatt cctgatacga tcgacaagcc cagtgtagaa gctttgttag ctacgaaagt
     7621 gctgcaacca aaacgaggag aaaagaaaaa attagttgaa ttggcgaata agaatgcctc
     7681 agttgctttg aatgaacggt ttgatctgat tgccagaaaa caggaacgga cgatcggagc
     7741 agttgaacgt ttaggaaacg cgatgaatat cccgacccca atacgtatcg agtcttttga
     7801 taactctaat atcatgggga cgaatcctgt ggctgcgatg gtcgtattca ttgatggaaa
     7861 accggcaaga aaagaatatc ggaaatataa aataaaaaca gtcaacggac cggatgatta
     7921 tgcatcaatg agagaagtca tctatcgcag atattcacga gtgattcgcg aagggttgcc
     7981 tttacctgat ttgatcttga tcgatggagg aaaaggacaa gtagatgtgg caaaagatgt
     8041 cttagaaaat caattaggaa tcgatattcc tattgctggc atggcaaaaa acgataaaca
     8101 taaaacaaat gagttgctat tcggtataga tttggctgta gtaccgcttg agcgaaattc
     8161 accagagttt ttcttactcc agcgaatcca agatgaagtc catcgattcg caatcacttt
     8221 ccatcggcag ctacgcagta aaaacagttt tgcttcccga ttagatgaaa ttgaagggtt
     8281 aggaccaaaa cgaaaaaaaa tgctgcttaa agagttcaaa tcgttgaaaa atatcacagc
     8341 agccacgaca gaagaattac aagaaatcgg actgccgaaa aatgtcgcac aaaacgtatt
     8401 tgataaatta catcaggaat gaggaaaggg cactttacaa agaaagtaaa gtgtcctatc
     8461 attttatgta caacaaaaag agcgtttacg ttttgttggt aaataataaa tcgcttacgt
     8521 ctggtgtcta aggatatatg tgatttcttg aagaaggagt gaagaaatgt tagaagtcaa
     8581 ggatcttgtt aaaacttttg ggaactacac agctgtcgat catgtctctt tccaaatccc
     8641 cgatggaaaa atcatggggc taattggcca aaacggggct gggaagacaa cgacttttcg
     8701 tttgatcttg gattttttga cacaagacca aggagaagtg ttatggaatg gccatcatct
     8761 tggggagaaa gattatgatg tcatcggtta tcttccggag gaacggggac tttatccgaa
     8821 agtaacgatt caagaccaac tgctttattt tgcagaactt agagggaaat caagaaaaga
     8881 gattgaacca aaaatcgatt tttggatgga aaaatttcag gtaaaaggga aaaagacgga
     8941 taaagtaaaa tctttgtcta aaggaaatca gcaaaaagtc cagctgatcg ccacgttaat
     9001 ccatgaacct aaattgatca ttttggatga gccattcagc gggttagatc cggtaaatgc
     9061 tgaattgcta aaagatggaa tattggaatt gaaaaaacat ggttcatgtg tcattttttc
     9121 tagccacaat atggataatg tagaaaaaat ctgcgatcat ttgatcatgt tgcgaaacgg
     9181 aaaaatggtt ttgaatggaa aagtacatga gattcgtgaa tcatttggcc gtacgaagtt
     9241 attcttggaa tctggtttaa gccaacagga agtagaagaa atcgaaggcg tgaaaaaagt
     9301 gatccttcat gaagacaata cgttagagat taccttagaa gatcctgaag ctgggaaaga
     9361 aatctttact cgtgcgactc agttcggcta cattcctatg tttaaccagc aaccgccaac
     9421 tttggaagag attttcaaat tgaaagcagg tgaaatcgat gcgtaagttt tggattatta
     9481 ccaaagatgt ctatttaaaa aatgttaagt cgatttcttt cttgatcatg atattagtcc
     9541 cttttatcat gctgggtatc atttatgtag caggaaactt tgctcaacaa aatagtgatg
     9601 tcaataaaat cggcgtattg gctgataatg agcaaatcag tcagcaaatg agccaagtca
     9661 aaacagatga ttttgagttc aaagcagttg attctgaaga aaaagcacaa aaagagcttt
     9721 cggaagaaaa aatcgatgcg tatttagtag tctcaactga gaatgataag ataaatggga
     9781 tgctttatag cgaaaattca ttaggacaaa gtacacaact actgatacaa caacaactga
     9841 ctggcttgca atcgatgctt cgagcaagtc aattaggcat ttcaccagaa gaagtagcta
     9901 gcttgagcca acaagcaagt ttgactcgac aaaaagtcaa ttttgattca aaaggaaaaa
     9961 tgacagttgg agaagataac tcgacagtcc aatatgtcgt aagttatgta gcaacgattg
    10021 tcttgttcat tattattttg acgtatgcgc agatcattgc acaagagatc gcttctgaaa
    10081 aaggcacgcg gatcatggaa gtgatcctgt ccagtacgac tgcacaaaag catttttacg
    10141 gaaaactaac aggtgtttta cttgttgcat taacgcaaat ggcattatac ggtattattt
    10201 ttggtatagg atacagtcaa ttcaaagata tggaaatggt caaaaatata ttagacggta
    10261 tctcattgga taacttgatt ggaccattct tgtggtattc tcttctattc atgatctttg
    10321 gcattttgat tttttctgtt ttagcagctt tatgtggctc tttggtcaac aaagcagaag
    10381 atacagcaaa agcaattttg ccagtgactt atctatctct aggcggatat atgcttggat
    10441 tgatactagg agcttcagat ccaaacaata tcgttattcg gatcacttcg tatattccat
    10501 tcttatcttc atatattatg ccggttcgct tagcaaatga gacagtcggt atgggtggag
    10561 cattgatttc cttgttggta cttgtagccg taacattctt attgatgatc ttgtctgcaa
    10621 acatgtacaa atcgaatgta ctcgtgtata gcgaaggcgg attatggagt tcattgaaac
    10681 aatcgatttc aattatgaga aatgaacgaa aaaaagcata aaatatagaa aaatatagaa
    10741 ggaagctgga catttattgt tcccagcttc ttttttgtat agacctattt ctattttttt
    10801 attatattgg atatatatat tcattgaaaa tagagcataa caactttata atgttgagta
    10861 taccaatttt tggaggcgtt ttaatggacc gctatttaga tttactttca gagaagttcc
    10921 ctacaacgga agatgtcatc acggaaatca tcaatctcga agcaattttg cagcttccaa
    10981 aaggaacaga gttatttttg agcgatatac atggcgaatt tcctgctttt gatcatattc
    11041 tgcgtatcgg ttcaggaaat cttaaagaaa agatcaaaga ccttttctct gaacaaatga
    11101 cggaaacgga gatcagccaa ttcactattt ttacggctta tccagaatat gtgttgacga
    11161 ctgattggta caaaaaacaa gataaatccc agctgatcca tcgtttgatc gatcttttac
    11221 ggtttacgac agtcaaatac actcgatcaa aagttcgaaa aggattgccg aaagaatata
    11281 gctatatcat tgaggaactc ttgtatatcg atgatcgtat caacgggaaa aaagactatg
    11341 taaaaaaaat cattgaacaa cttgtcttga tgaaggaaga agaaaaattt ttaacaaaac
    11401 ttgcgcagac gatccaaaaa tctgtcatcg atcatttgca tatcgtgggt gatatttttg
    11461 atcgaggaac tcaagcagcc aaagtaatgg atcgaatcat ggatttttcc tctgtagata
    11521 tccagtgggg gaatcatgat attttgtgga ttggtgcgta ttgcggctct gaggcatgtt
    11581 tattgacgct tttacggatt gccgcacgct acaactattt atttgaatta gagaaagaat
    11641 atggattaaa tttacgtcca ttattttctt ttgcccatca aacctatcag aagaatccag
    11701 tatttacccc taaaaacagg aaagatctat ctgaaagaga gcaagaagaa ttagaaaaga
    11761 tccatcaagc actttctatc ctccaattca aatcagaaca tcagttgttg aatcgacgac
    11821 ctgaatttaa aatggacgat cgtaaattgt tggaaaaaat caactatgag gaatcaacga
    11881 tcgttttaga aggtcagctc tatgagttga aagggacttg ttttcaaacg atccagccag
    11941 atgatcctaa aaaattcttg gaagaggaac aaaaagtgat cgatagtttg atgtattcat
    12001 tccagcactc gttaagaatg caaaaacaca tgacgttttt gatcgaaaaa gggggaatgt
    12061 atctcacgaa caatcagcat gtactgttcc atggctgtat acctgtagat gaaaaggggc
    12121 agctcttagc atttgaatta gaccaatctt atcgcggaaa agaactgctg ggattttttg
    12181 aaaaacagat aactgaaagc gcaaaagatt tgactgcaaa tgaagatttg gcgaccgatt
    12241 tgatttggta tgcgtggagc gggccatttt cgcctttatt cggcaagagt aaaatggcaa
    12301 cttttgagcg ctattttctc acagagcctc atacgcacgt cgaaaaaagc aatccctatt
    12361 atcacttgcg agatgaagag tggttcagtg aaaaaatttt ggcagaattc gatgcaaaag
    12421 aagaaggttc agcaatcatc aatggacaca cgccagtaaa agtaaaaaaa ggagagtcac
    12481 cgatcaaagc aaacggaaaa gcatttgtta ttgacggagg tctatccaaa gcttatcaaa
    12541 aaacaaccgg gattgctggc tactcattac tatgcaattc ttacggtttc caaatcgtga
    12601 cacattatcc gttcttgcct attgaacaac aatttgcaaa aaaatctgat caaacgaatg
    12661 taaaaaaagt gatcgagcag cagctcccac gcaaacttaa tcggaatacg acaaaaggaa
    12721 tggaattaca gcaacagatt actcaactcc aaaggctgtt ggattatcga aaggacgatt
    12781 gagaaaaatg ctagccaaca ttgctcaata gactaaaaag cgactgcaac aaaaggcgtg
    12841 tcgcagtctc cttcggaatt agctcaaaaa acggtaaaat attagaagct atttttctgt
    12901 ttatttctct aatactcagg tcagggcgct gatttcacga cctcttcctt aaacggtgtt
    12961 ctcctgccaa ctcctcggtc acaagtcaca atatccaaca aacatgagaa gctgtttttt
    13021 gaaattgctc cttgtgactc agagttgaac ggcaggctca cgacctaatc agaaaaacga
    13081 ttaaaattca ttcacaaaga aatcgaataa acgctgttca gatgaatcta cacgatgaga
    13141 aagttccgga tgccattgga caccaaggat tttgtgcata ttatctttac tttggactgc
    13201 ttcaatgagt ccatcgttgg aaaaggcgat ggcttttaac aaaggagaaa gatctttgac
    13261 tgcctgatga tgataggaat tcacaaagta tgaatcaggg agaaggtcac ttaataaact
    13321 gtccgaaacg attttgactt cgtgagttgc aaactgcggg gcagtgggct gttgttcgtg
    13381 ttttacggac cattttggat ataatgaaag atcttgataa agtgttccct ccaacacgac
    13441 attcaatagt tgaagtcctc ggcagactgc aaaaatcggt tttctttgtt tcaatgcttc
    13501 caagatcaat gcctgttcga attggtctcg ttgaatattt gtttccgtca gttttggatg
    13561 agggtcttcc atgtaaagtt gcggactaac atcctgaccg cctgcaagaa tgagtttgtc
    13621 gattttctca attagttgag gcgctgaatc tggagacatg actggcagaa taagaggtaa
    13681 tccttgagca tctaaaactg catccacaaa tccctgcggt gtatagctta cttgatttcc
    13741 ttggaaagta tctgtcgctc gaatcagttg attcccggca atgccgatga ttggtttcat
    13801 ttttattccc cctaaagtag actaattgta acaaggattt aaaaaaattt aaactatgta
    13861 agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga gaattttcgt
    13921 gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa aagtaagcag
    13981 aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg agaaggacat
    14041 tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt tcaatgaaaa
    14101 aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt agaagctttg
    14161 gaacaaatcc gaaaagcaga agaagaaaat gagaagcgtg ctcgaaccct acggcagcag
    14221 ttgaagacct atgcagaaga aaaagagcaa gaattggaga taatccaaac gaacagtcgg
    14281 caacagctga aacagttggt agaagaaaaa gaacaattcg aaaagcaaga attagaaaaa
    14341 gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa agaccaatat
    14401 caaaagcatc agagtcaagc aatcggtgca atcattgaaa gggtgaaaga aacttatggc
    14461 cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg agaggatttt
    14521 acaagcagta caaggaatcc aaaatgttga aatccgggat ctgtatcaaa atagaacaaa
    14581 caaccagtgg gtagaaaaat attttccaga taccacgatt attgataaag aagcaaaatt
    14641 aagcgcttta ggcaatcggc tgaaagacat ccaagaagct atccaattca tacaacacca
    14701 cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct tacaagactt
    14761 ggaagcaaat tattccgaag aaaatttcat gaataaatta gaagaagtcc ttgatttaaa
    14821 agaaaagtgg gaacagctaa ctgaaaaacg tgatcgttta gaagaagagg aagaatggct
    14881 tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga cgaccatttt
    14941 tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac taagtaagct
    15001 tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc tttcgtatgt
    15061 tgctttaaaa aaatcagttg ctgctataga agaaattgct gggaaatacg ggttcaataa
    15121 agaaacgtat ccttatgaac aaaatcctaa agaaaaattg caagaaacca aacagcaatt
    15181 atccattgtt cacgagcagc aaaaaaagat aacgacagcg ttaggtcagt gcagcggcta
    15241 tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac gggaaaaagt
    15301 aaaagaacgg tttatccatg ccacttatct gatcgttttg caaggttggg tagatgcaga
    15361 agaaaaacag aatttgatgc atgtgttaaa cgaaactgta tcagaagaag atatatacgt
    15421 tagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta aattgaaaaa
    15481 ccatccgctt atcgaacctt ttgaattgct gactgaaatg tacagtctgc caaaatatga
    15541 agaagtagat ccaacgcctt ggatgacacc attttatctt gtcttcttcg ggatgatggt
    15601 cgcagatgtt ggttacggat tgctgatgct cattgggaca atactagctc aaaaactatt
    15661 tgttttaccc cgcgggatga aacgtttcgt aaaattcttt gagattctct cggtaccatc
    15721 gatcatctgg ggattgatct acagttcatt tttcggacag gcattaccaa aagaactatt
    15781 tggtatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata cgattttgat
    15841 tttatccgtg attttcggtc tgatacagat cttggttgga ttattcgtgg cagcaaagga
    15901 aaatatcagg aggaaagatt acttgagtgc tgtcagtgat ggttttgcct ggcaagggat
    15961 cctgattggg atcgttattg cattgattgg tgtcatgttg ataaaaaata cagcatttgt
    16021 ttatcttggc gcggggctat cgatcattgc tgctttatgt atcttaatcg tgccgattat
    16081 ccaatcgccg tctaaagtca agggtgctgc caaaggagcg tataatcttt acggattgac
    16141 tggttatatc ggtgatttag tcagttatac acgtttgatg gcactaggta tttcaggcgg
    16201 aagtattggc gcggcattca atatgctagt tgctttcatg ccgccagccg ctcgattcag
    16261 tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga cactgttaag
    16321 tgcctatgtc catggtgcac gattgcagta tgtcgaattt tttggaaaat tttatacagg
    16381 aggcggacgt gcctttgacc cgctgaaaac ggcggaaaaa tacgtcaaca tcaatcataa
    16441 gaaaaaaaac aataaataat tggaggattt ttatacaatg atggattact taattagtca
    16501 aaatggtgga atggtatttg cagtattagc gatggcaaca gcgacgattt tttcagggat
    16561 tggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcgttaa caacaagtca
    16621 acccgaaaaa ttcggacagg ctttgattct gcaattgctt ccaggtacgc aagggttgta
    16681 tggattcgtt atcgcctttt tgatttttat caacttagga aatgatatgt cagttgtaca
    16741 aggattgaac tttctaggcg cttctctgcc gattgctttt acaggtttgt tttcaggtat
    16801 cgcccaaggg aaagttgcag cagcaggtat ccaaatttta gcgaaaaaac ctgaacatgc
    16861 tacaaaagga atcatttttg ctgcgatggt tgaaacatat gcgattttag gtttcgttat
    16921 ttccttccta ttagtattga atgcctaagc ataaaggagg aaaagcagat gaacgccatt
    16981 gaaaaaatca tctctcagat gaatgaagca gcagagcaag aaagagcggc gttagagcaa
    17041 gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaaat cgaaacagaa
    17101 catcaaaaac aaaaagaaaa acaaatcgaa ttacttgaaa aaaaataccg tcagcttagg
    17161 aatcgccaac aagtagaagt acgacaagaa aatttgaatg cgaagcagga atttttacga
    17221 cgattgtttg cagatgcaat agctgaaatg gagaattggg atgaatctga acaaatccag
    17281 tttatcaaaa attcattgta ttcattacct ttgacaggca aagtaacatt cattgctgga
    17341 gaaaaatcag cagcttatct ttctcagaca ttgctggatg aatggaacaa tgaacttccc
    17401 tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct gataaacgat
    17461 caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca aggaacgatg
    17521 agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg gaataccacg
    17581 aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag acatttgaac
    17641 ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc acgatttaca
    17701 gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta aaggagcaga
    17761 gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt tggatctata
    17821 cgatgcgcta tactttccat aatctgaagg ttttgacaaa agccgagatg acaaatcaaa
    17881 atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg aaggatgcca
    17941 ttcatacgca agtttcaaat gaactccctg aaagcattat ggacagtata cgagaagtac
    18001 gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat gatcgacagt
    18061 ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg ctgacagaag
    18121 tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata ttgcagaaaa
    18181 gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa aaagaagtat
    18241 ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta gagacagatt
    18301 atgctgattt tctgcgccct gtcatccgtg agggaaatct tgatctcgta aagcttgaac
    18361 agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag gctttcggac
    18421 cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac cttcgtttac
    18481 tggttatcgg caaaagaagc catttcgaac cagaaacaat cagaaagcgg gtgagacaag
    18541 tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc attcaaattg
    18601 tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc aattgaagaa
    18661 atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc attgagccca
    18721 gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat tcccaatcat
    18781 caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa agcagtaggt
    18841 caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa aatcataaaa
    18901 gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca agacatgtgt
    18961 ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga tgtggcgtct
    19021 attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg ctctactggt
    19081 gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga cggaatccaa
    19141 cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg cggagttcaa
    19201 ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga agccggagaa
    19261 acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat aaaacataaa
    19321 atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg ttcattcact
    19381 attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt gacgatgctt
    19441 caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc ggatgcaccc
    19501 atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg aggagcagca
    19561 gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat tgcaaaatgg
    19621 gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga gatgacggat
    19681 gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt gatggaacgt
    19741 acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc ttctatctat
    19801 acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc tatcatggca
    19861 gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt ggaagaaatg
    19921 cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata ctatgaacga
    19981 tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac cgcaatcagt
    20041 gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac attgcgggtc
    20101 gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt tccttcgatc
    20161 gattggatac aaagctattc tctttattca acagaagtag ggaagtatat ggatcatatc
    20221 cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca ggaagaacag
    20281 cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa tgaccgtctg
    20341 acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc ttttgatgat
    20401 gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat cttgaccttt
    20461 ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat catggacggt
    20521 accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac agaattagaa
    20581 aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt ttcagaagga
    20641 ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc cattgatggc
    20701 ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta tgcaaaatgg
    20761 cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg tccaaatatt
    20821 cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag gccatccttt
    20881 agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg gacgaccgaa
    20941 agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag aagtaatcaa
    21001 tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg caatcgatca
    21061 tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg gtcttcctca
    21121 taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat cagatgattt
    21181 cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt tcatggaaga
    21241 ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag caaacgaccc
    21301 tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat atcttgctta
    21361 tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg cagaggcatt
    21421 gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc ctgggtatct
    21481 ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac taaaaggatc
    21541 ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc caatccctga
    21601 cttgactggt tatattacag aaggacagat catcttgact agagagcttt acaagagtgg
    21661 gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata agggaactgg
    21721 acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg cagcctatgc
    21781 acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt tatctgatgt
    21841 agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca atcaaggatt
    21901 ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc tatcgatgtt
    21961 gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc tgccagaagg
    22021 gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat tgactcgatt
    22081 gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata agcaagatga
    22141 actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc aagaaatgga
    22201 agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga tcacggaacc
    22261 tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta tcgtagacaa
    22321 aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac agctgacaga
    22381 cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt caatcgatcg
    22441 gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa cttgtcaact
    22501 gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat atatgacgat
    22561 ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa acgagcgcgc
    22621 agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag tggtatcttt
    22681 ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg tttgccggct
    22741 gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg cctacttacc
    22801 ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct tttcactgca
    22861 acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca ttggaacagt
    22921 gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt tatgatggtt
    22981 ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg tattatatta
    23041 aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct ttacatccta
    23101 aaattggcct ttgttatcca attgattggt acggttgctt tatcttttgt atttgtgcct
    23161 gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc tagtttttgt
    23221 aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa tgtttatttg
    23281 atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt atggcgtgat
    23341 ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc attaaccgtt
    23401 acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg caatggggca
    23461 acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat gagtgtgaca
    23521 cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc aggtctgatt
    23581 ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg cggattgaaa
    23641 acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg acgtacgaga
    23701 gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact tactttattt
    23761 tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac tgaaacgata
    23821 cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt tggtacagta
    23881 ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat cattatagcg
    23941 cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat gacaaaagca
    24001 caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg ataatttcat
    24061 cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt ttcttgcttc
    24121 ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg ttgttgaacc
    24181 tcgggtagtg cctctt
//
LOCUS       DAJSMI010000023        23967 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJSMI010000023 DAJSMI010000000
VERSION     DAJSMI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.023"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            143..258
                     /gene="rrf"
                     /locus_tag="ON430_000779"
     rRNA            143..258
                     /gene="rrf"
                     /locus_tag="ON430_000779"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            268..341
                     /locus_tag="ON430_000780"
     tRNA            268..341
                     /locus_tag="ON430_000780"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Asn,seq:gtt)
     gene            381..470
                     /locus_tag="ON430_000781"
     tRNA            381..470
                     /locus_tag="ON430_000781"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:415..417,aa:Ser,seq:gga)
     gene            485..556
                     /locus_tag="ON430_000782"
     tRNA            485..556
                     /locus_tag="ON430_000782"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:518..520,aa:Glu,seq:ttc)
     gene            572..644
                     /locus_tag="ON430_000783"
     tRNA            572..644
                     /locus_tag="ON430_000783"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Val,seq:tac)
     gene            654..726
                     /locus_tag="ON430_000784"
     tRNA            654..726
                     /locus_tag="ON430_000784"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:687..689,aa:Asp,seq:gtc)
     gene            762..837
                     /locus_tag="ON430_000785"
     tRNA            762..837
                     /locus_tag="ON430_000785"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:795..797,aa:Phe,seq:gaa)
     gene            839..919
                     /locus_tag="ON430_000786"
     tRNA            839..919
                     /locus_tag="ON430_000786"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:873..875,aa:Tyr,seq:gta)
     gene            926..997
                     /locus_tag="ON430_000787"
     tRNA            926..997
                     /locus_tag="ON430_000787"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:958..960,aa:Gln,seq:ttg)
     gene            1122..1292
                     /locus_tag="ON430_000788"
     CDS             1122..1292
                     /locus_tag="ON430_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218722.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            1512..2219
                     /locus_tag="ON430_000789"
     CDS             1512..2219
                     /locus_tag="ON430_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3218723.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(2279..2923)
                     /locus_tag="ON430_000790"
     CDS             complement(2279..2923)
                     /locus_tag="ON430_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCR3218724.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            3050..3337
                     /locus_tag="ON430_000791"
     CDS             3050..3337
                     /locus_tag="ON430_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218725.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            3445..3765
                     /locus_tag="ON430_000792"
     CDS             3445..3765
                     /locus_tag="ON430_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3218726.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            3846..5159
                     /locus_tag="ON430_000793"
     CDS             3846..5159
                     /locus_tag="ON430_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3218727.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            5156..5875
                     /locus_tag="ON430_000794"
     CDS             5156..5875
                     /locus_tag="ON430_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCR3218728.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            6003..6554
                     /gene="raiA"
                     /locus_tag="ON430_000795"
     CDS             6003..6554
                     /gene="raiA"
                     /locus_tag="ON430_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR3218729.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            6776..9310
                     /gene="secA"
                     /locus_tag="ON430_000796"
     CDS             6776..9310
                     /gene="secA"
                     /locus_tag="ON430_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR3218730.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            9436..10537
                     /gene="prfB"
                     /locus_tag="ON430_000797"
     CDS             join(9436..9507,9509..10537)
                     /gene="prfB"
                     /locus_tag="ON430_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR3218731.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            10642..11328
                     /gene="ftsE"
                     /locus_tag="ON430_000798"
     CDS             10642..11328
                     /gene="ftsE"
                     /locus_tag="ON430_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR3218732.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            11312..12205
                     /locus_tag="ON430_000799"
     CDS             11312..12205
                     /locus_tag="ON430_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218733.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            12357..13805
                     /gene="cls"
                     /locus_tag="ON430_000800"
     CDS             12357..13805
                     /gene="cls"
                     /locus_tag="ON430_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR3218734.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            14142..14999
                     /locus_tag="ON430_000801"
     CDS             14142..14999
                     /locus_tag="ON430_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR3218735.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            15114..16034
                     /gene="pstC"
                     /locus_tag="ON430_000802"
     CDS             15114..16034
                     /gene="pstC"
                     /locus_tag="ON430_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCR3218736.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            16034..16918
                     /gene="pstA"
                     /locus_tag="ON430_000803"
     CDS             16034..16918
                     /gene="pstA"
                     /locus_tag="ON430_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR3218737.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            16933..17739
                     /gene="pstB"
                     /locus_tag="ON430_000804"
     CDS             16933..17739
                     /gene="pstB"
                     /locus_tag="ON430_000804"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR3218738.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            17755..18513
                     /locus_tag="ON430_000805"
     CDS             17755..18513
                     /locus_tag="ON430_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR3218739.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            18526..19203
                     /gene="phoU"
                     /locus_tag="ON430_000806"
     CDS             18526..19203
                     /gene="phoU"
                     /locus_tag="ON430_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR3218740.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            19441..20472
                     /locus_tag="ON430_000807"
     CDS             19441..20472
                     /locus_tag="ON430_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR3218741.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            20469..21236
                     /locus_tag="ON430_000808"
     CDS             20469..21236
                     /locus_tag="ON430_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR3218742.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            21336..22904
                     /gene="liaX"
                     /locus_tag="ON430_000809"
     CDS             21336..22904
                     /gene="liaX"
                     /locus_tag="ON430_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCR3218743.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            23201..23521
                     /locus_tag="ON430_000810"
     CDS             23201..23521
                     /locus_tag="ON430_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCR3218744.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            23521..23871
                     /locus_tag="ON430_000811"
     CDS             23521..23871
                     /locus_tag="ON430_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR3218745.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         7798 a         4169 c         5212 g         6788 t
ORIGIN      
        1 agtgatgtat ggagcggact gatactaatc gatcgaggac ttaaccaata ggttaaaaga
       61 aaagtactcg gaaaaaaact tatgtgactt caaatccagt tttgagtgaa caaaatttac
      121 tcaataaaag ataacaccac agtgtggtgg cgatagcgag aaggatacac ctgtaaccat
      181 gccgaacaca gaagttaagc ttcttagcgc cgattgtagt gaggggttgc cccttgtgag
      241 agtaggacgt cgccacgctg atgttattcc ggcatagctc agttggtagt agcgcatgac
      301 tgttaatcat gatgtcgtag gttcgagtcc tactgccgga gttctcattt tgagaaagag
      361 tattgagctt ctttcactct ggagagttgt ccgagaggcc gaaggagcat gattggaaat
      421 catgtagatg gtgccaaact gtctcaaggg ttcgaatccc ttactctccg tagcaaattt
      481 acctggcccg ttggtcaagc ggttaagaca ccgccctttc acggcggtaa cacgggttcg
      541 aatcccgtac gggtcatttt aattgaatgt tggaggttta gctcagctgg gagagcatct
      601 gccttacaag cagagggtca gcggttcgat cccgttaacc tccataatga actggttccg
      661 tggtgtaggg gttaacatgc ctgcctgtca cgcaggagat cgcgggttcg attcccgtcg
      721 gaaccgtcgt tcttgttatt caattaataa ttttaaaata tggcgcggta gctcagttgg
      781 tagagcaacg gattgaagct ccgtgtgtcg gcagttcgat tctgtcccgc gccaccatgg
      841 aggagtagcg aagtggctaa acgcgacgga ctgtaaatcc gttccttagg gttcagtggt
      901 tcgaatccac tctcctccat ttcattgggg tatagccaag cggtaaggca agggactttg
      961 actccctcat gcgttggttc gaatccagct accccagtat accacaatcg taagctctcg
     1021 ttagttcgag agctcttttt tttgctttgc taccttgttc cttgaagatt ctgctgattt
     1081 aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg aaagaaaaag
     1141 tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac aatattgaag
     1201 tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc ccaacgaatc
     1261 atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat aagtcggcat
     1321 tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa aaaattgttt
     1381 ctgacaatag gagtaggaag aacgatgtgt gtctaaatcc tctgttgatg gatagaaacc
     1441 tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa acgaaaggac
     1501 gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac caattaaaaa
     1561 atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa agagaattag
     1621 cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac ttggcagatg
     1681 aaggaatctt agaaagaaag atcggttcgg gtacatatgt tgcgcgagaa aaagtacaag
     1741 aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcgcaaaat cgagtaccgt
     1801 ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg gaaaaactaa
     1861 acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca gatgatgtcc
     1921 ctatttgttt tgaagttgcc agtgtgccac aaaagctgat cgatggctac agcaaagcag
     1981 agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag atcggtccag
     2041 cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat ttagaggtaa
     2101 aacgaggaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac ggtacgccgt
     2161 ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg gaaaaataaa
     2221 aaacaggtga tccctgattc cttacttaga aataaaagag aatcagaatc acctgttttt
     2281 atatatgatc agttaccagt tgctcgtgat aagaaacagg accctcttca aattggactt
     2341 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     2401 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     2461 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     2521 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagacagca tgagtgtatg
     2581 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     2641 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgcca gccgttccac
     2701 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     2761 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     2821 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     2881 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     2941 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     3001 caatcgctgt agtatgcgat aataaagaca aaggtaggtg gatctgggga tgcaggaacg
     3061 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     3121 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     3181 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     3241 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     3301 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     3361 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcgtagt
     3421 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     3481 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     3541 gactcggata ttttcgctgt gtccgcttct gacgcagata acaatcttgc aaataaagat
     3601 gtagatgtac tgttattagg acctcaagtt cgctttatga aagctgattt tgaaaaacgt
     3661 ctggaaccaa aaggcattcc tttagacgtc atcaatatgg ctgattatgg tatgatgaat
     3721 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     3781 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     3841 ataatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattaaa gcaatagact
     3901 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     3961 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     4021 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     4081 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     4141 ttactgaaaa acaaacggaa atcgcaaaga gaatactttg ccattcagaa aaaagacatc
     4201 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     4261 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     4321 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     4381 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     4441 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     4501 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     4561 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     4621 acaaacttgc tttgcgcttt catcggcgtt tacttcctga acccgtcttg gttttcatga
     4681 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     4741 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     4801 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     4861 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gacatactgt
     4921 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     4981 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     5041 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     5101 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     5161 gtgcagttgc tgcagtaaag atatcgtcca aaatttaaca ttcagcgaga ttattggtgt
     5221 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     5281 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     5341 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     5401 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     5461 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     5521 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     5581 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     5641 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     5701 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     5761 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     5821 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttagcaa gatgaattag
     5881 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     5941 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     6001 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     6061 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     6121 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     6181 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     6241 tggttgttga caaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     6301 cccgtgaaac gggcatgaat actgcagatg ctactgcttt attcaatgaa gaggacgaaa
     6361 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     6421 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     6481 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     6541 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     6601 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     6661 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     6721 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     6781 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     6841 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     6901 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     6961 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccaca
     7021 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     7081 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     7141 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     7201 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     7261 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     7321 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     7381 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     7441 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     7501 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     7561 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     7621 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     7681 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     7741 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     7801 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     7861 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     7921 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     7981 tcgtccagat ttgctttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     8041 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     8101 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     8161 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     8221 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     8281 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     8341 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca cagttctact tgtcattgga
     8401 agatgatttg atgaaacgat tcggttcaga acgaatcaaa tctttcttag atcggatgaa
     8461 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     8521 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     8581 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     8641 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     8701 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     8761 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacagctca
     8821 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     8881 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     8941 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     9001 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     9061 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     9121 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     9181 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     9241 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     9301 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     9361 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     9421 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     9481 aaggtcacaa gcttcaggag gtctctttga cttagaccag ctggaagaag acattgcgga
     9541 agcagaagca aaaatggctg aacctggttt ttgggatgat agcgaagctg ctcaaaaagt
     9601 gatcagtgaa aacaatgcaa ataaagaaac ttatgatcaa ttccattctc ttgctgaaga
     9661 actggaagaa atcgaattga tggtggagat gttgcaggaa gaacctgatg cagccatgca
     9721 agaagaagca gaaacacgta tcaatgcgct agatgaaaaa atgaagacat atgaattatc
     9781 tatgttgtta gatggtcctt atgatcgaaa caacgccatc gttgaattgc atccaggggc
     9841 tggcggaacg gagtcccaag attggggcag tatgctttta cgtatgtata cacgctgggc
     9901 agagcagcat gggtatcaag tagagacact agattatcaa gcaggtgatg aagcaggaat
     9961 caagagtgtg accctactga tcaaaggaca caacgcattt ggttatctga aatcagaaaa
    10021 aggtgttcat cgccttgtac gtatctcgcc ttttgactca gcgaaacgac gtcatacttc
    10081 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
    10141 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
    10201 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
    10261 acaacgttca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
    10321 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
    10381 aatcggctgg ggttcacaga tccgttctta tgttttccat ccatattcta tggtaaaaga
    10441 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
    10501 atttattgac ggctatttaa aaatgcatct acaataagaa cccattgtta aattgaaata
    10561 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
    10621 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
    10681 ggtacgaccg ctatccgcaa tctctcaatt gtcatcgaac aaggagagtt tgtctatgtg
    10741 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
    10801 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
    10861 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
    10921 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgggat
    10981 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    11041 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatagtg
    11101 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    11161 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    11221 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    11281 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    11341 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    11401 cagtgcggta acgatcacat tgactttagt cggtgtattc atggcagtta tcatgaacgc
    11461 aactaaatta gctcaagata tagaaggaaa cgttgatgtc tctgtattcg tagatatcgg
    11521 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    11581 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    11641 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    11701 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    11761 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    11821 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    11881 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    11941 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    12001 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    12061 caatccatca ttattacgtt cccattattc attgattcat ccacaaaata tcgtttggaa
    12121 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    12181 tatgagacgt ttcttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    12241 tttcgctcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    12301 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    12361 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    12421 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatgggtg ctcgttttga
    12481 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    12541 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    12601 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    12661 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    12721 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    12781 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    12841 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    12901 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    12961 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    13021 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    13081 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    13141 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    13201 atacaaaaga agtaggctat tctcccaaat tttttccgtc cattcactct actggtgata
    13261 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    13321 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    13381 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    13441 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    13501 cagagctaat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    13561 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    13621 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    13681 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    13741 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    13801 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    13861 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    13921 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    13981 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    14041 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    14101 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    14161 tcttaccaat aataggatta gctattcttt tatcaggctg ttcaaagtgg attgatcgag
    14221 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    14281 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    14341 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    14401 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    14461 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    14521 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    14581 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    14641 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    14701 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    14761 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    14821 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    14881 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    14941 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    15001 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    15061 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    15121 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    15181 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    15241 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    15301 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    15361 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    15421 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    15481 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    15541 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gactgggttt ggtatcttag
    15601 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    15661 cattaaaggc agtgccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    15721 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    15781 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    15841 cagcattgat gccaacaggt cttactactc ctgcttctac attgacgtcc atcttaacaa
    15901 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    15961 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    16021 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    16081 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    16141 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggcgg
    16201 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    16261 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    16321 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    16381 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    16441 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    16501 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    16561 aacagtgata aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    16621 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    16681 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    16741 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    16801 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg attattgctg ttttactgtt
    16861 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    16921 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    16981 gagaaagaag tagcactata tacggatgat ctacatgtct ggtatggcga caatgaagca
    17041 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    17101 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    17161 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    17221 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    17281 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    17341 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    17401 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgtattgct
    17461 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    17521 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    17581 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    17641 tatttaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    17701 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    17761 aaagagatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    17821 gggatcgatc taagcttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    17881 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    17941 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    18001 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    18061 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    18121 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    18181 cataaaagtg ctttatcttt atccggtggg cagcagcagc gtgtatgtat cgcacgtgtt
    18241 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    18301 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    18361 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    18421 ggtcacttga tcgaatacaa tgatacgaag aagatattca tgaatccaga aaaacaagag
    18481 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    18541 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    18601 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    18661 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    18721 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    18781 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    18841 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    18901 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    18961 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    19021 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    19081 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    19141 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    19201 taaactgaag ctgtgattat gccgtttgtt taggaaaaga ggttgtgaaa tcagcggttg
    19261 tgaaaaagct gttctgcatc aagtaataag aacagtcaaa caaaaaatag ctcctcatat
    19321 ttttcgtttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcggga
    19381 aagggtttgt gacaaaacat gtcacaaacc cttttcatca tttcatagaa aggataaaga
    19441 atgcaaaaaa aaattagcat catcactata ttttttattt gtgtgcttac ttcatgggtt
    19501 attcagcatc aagccgcttt atatgaagta cctgtcatcg agatagaaaa agtacgaata
    19561 aaagaggatc atcaattgat tacaggaaaa ctgatgaaca cggatcaaaa aggagaatcg
    19621 atttatttat cggttcctta tcaaaaaaat caaatagaaa cagcaaatat aaaagcgaga
    19681 caaatgtatc tagtccaaat gcatggagat gaatggcagc tagtagcaaa aaaaaatgat
    19741 ggctggcttt tcttcttcac aagtatcttc attggcgcca tgttgcttat tggtggaaaa
    19801 cagggtctat ttgctttagg aagtctcatc atcaatatcg ttttactttt acttttgttg
    19861 ctcatcttta ctaaaacaga agggatttct ctattaaata tatctatcct gttcgtcgtt
    19921 atagggatga ttattagcat attggcttta gatggatgga atagaagcag tataatcaaa
    19981 atttcagctg cagttacgag tgttttttta gcatttttta tttgtctttt gacaatgaat
    20041 cattggaaag accaaggact gcgctatgag gaactgaatt atctgacacg accgtatcgt
    20101 tctgtttttc tttctagcgt actgattgga acagctggag gaacactcga taccgttatc
    20161 acagtcattg cgacattaga agaattgact aagcgacaac cagaaataac agcgaaacag
    20221 ttatggcttt caggaaggca agtgggtcga gatgtgattg gctcgatgac caatatcctt
    20281 ttgttcgtct atatcagtgg gtcagttcca tcgcttcttt tatatttagg aaatcaatgg
    20341 tcgttcaaag aaacgataga acagcattta tcattggaat ttttacgcgt cattgctggc
    20401 agcttaggaa tagtattatc gatccctatc tcggtcctct tttttctgac tgtaaggagg
    20461 ctgaaacgat gaatgtactt attatacttg ttgggatttt agcaatcagc gtgctactta
    20521 ttggcggtac gcaaggtatg tatattcttt taggcttatt catcaattta gggatctttt
    20581 tcctgttgtt attcggttat catcagaagt ggccgatact ggcattgtcg gtcataggat
    20641 ttttattgat tgctgtggtt atcttatttt ttatcaacgg ttataatcta aaaatgcgtg
    20701 cagcttttgt gagtatactg atctttttat tttgtttcct actattgctt cctatcactg
    20761 attttttagc aatccaaggt tttacatcgg tagaactaga agaattatct ggcttagata
    20821 agactttagc gattgatttt agattactta cgagatcgtt gcttctgatt agtcttagtg
    20881 gtgctgtttt agatgcgagt gtagcaattt ctagtgggac attcgaagta tatcaggcga
    20941 atccgcaatt atctttttac caattgcgcc attctagttt cgctattgct aaaaaaatat
    21001 tggcgtcaac agtaatgaca cttctttttg cattcatggg aagttcttta gcgttgatcc
    21061 tctggtttat tgatttagac attccttttc agcaagtgat caatgaaaaa tcatttgttc
    21121 tagaatatac gatggctatt cttaccacat tgtccgcact acttgttctt cctttaacct
    21181 gcgtgactac agcatatctc tttacaaaaa agaaagaaca actcaagatg gagtaaaagt
    21241 tacaacttaa gtcctatgac atttttactg aatcagtgca taatatggat gtaaggaaaa
    21301 gcttgcaaac aattttgctt aaggaggcaa tttccatgaa agaaagagaa cgtattttag
    21361 atttagtgaa aaaaggaatc ctttcgactg aagaaggctt agatttatta gaaagcatgg
    21421 caactgaaaa agacgaaaag caaatcaaaa aagaagcaga caaggtaact gctagtcata
    21481 aagaaaaaga tcaggcaagt caattgatcg ataaacttga aaacggcgaa gaagaaattt
    21541 ctgagccagt tgatccaaag gaaagagaaa gacaagatca agaaaactta gaaaagatct
    21601 tagatgaact agcaactgaa gcgaataaaa cttcggcgcg tctagatgaa gtaaatgctc
    21661 aattcgctga caataaggca gcacgcaatg agaaacagga agcattgatg cagctgaata
    21721 caaaagaaga attaggcgag ttgacagaag aagaattagc acagcgtcag acgttagaag
    21781 cagaaatcaa agaattagaa gctagtggcg atacactgtt agaagaacaa ttgaaattag
    21841 aagcagaatt aaaagatatc cgtaagaatc aatggtctga gaaaaaagaa acgtttactg
    21901 ataagtttga attgccagat gattggaaag atcaagcaac agatacactg aatcaagtag
    21961 gcgaaaaaat gtcagaagct ggatcacagt taggcaaatt cttgaagaaa acattccaaa
    22021 ctgtttcaga aacagtgaat gacaacatgg aatggaaaga tgtcagctta cgtgtccctg
    22081 ggattgcgac aacgaaattt gagcatgaat tttactatga agctcctgca gcaagtatcc
    22141 ttgatatcaa ggcagcaaac ggaaacgtga cgttaaaaac gtgggacagc aacgatgtga
    22201 aagttgaagc taagatcaaa ctttacggaa aaatgggtgc tgaaccattt gaagcattct
    22261 ctgaacggag ccagattgag gttaatgaag atcatatctc cttccaaatt ccaaataaac
    22321 gtgtgcgtgc agaccttgtt ttctacttac ctaaacgagt atacgaccac gcagctatta
    22381 aacttctaaa tggcaacatc atgattgaaa cattggaagc taaagatatt tatacaaaat
    22441 ctacgaatgg taacatcatc gtggaccaat taactgcaac gatgctggaa gtagaaggtg
    22501 taaacggcaa tatcgatatt cgaaacggga acatcttaga ttctattatc gaaacagtaa
    22561 atggtacagt gacatttggt gcgacagcag aaaacctttc tgtgtctttg gtaaatggtg
    22621 atgtacgtct tacgattaaa gaagataatt tgaaaaaagt agaagcaagt tcagtgaacg
    22681 gaaatgtcaa agtcgcttta ccagatggca tcggtttgga aggacatgct aaaacaagtc
    22741 ttggaagtat caacagccgt ctgtccaatt acgaagtagt tcgtgagaaa aaagaacgga
    22801 caaaccaaat gttgcaattc cgtcgtgttt cagatggtga aatcgcgcaa gtgcaacttt
    22861 caacgacaac aggaagtatc tatttaaaag atacagataa ataaagaggt tgtgaaagag
    22921 atgttcagcc ttgagcaata agaagcgatt ttgtaaaata gcttccccta ttttacaaaa
    22981 ttgtgcctta ttgccgatag tctatctctt gaacaccgtt taccaagaga ttatgaacct
    23041 gccaattatc gccgagtcac aaggaataat ttcagaaaaa gctcgtcacg ttttatgaat
    23101 atggtaattt gtgaccgaga attttgcaga aagacaccga ttaagaagaa ggttgtcaca
    23161 acgattttct tcatttaaaa tagtagaaca gaggtgagac atgatgaaaa aaaagttgac
    23221 aaaatctaat aaaaatgttg ttttgacagg gactttagca ggaataggag aatacatcgg
    23281 gattgatccg actgttatcc gtgtgcttta tgtattcctg agcttagttg cttttggaag
    23341 tccaattatt ttatacattt tattagcttt gattatccca tctgcgcgtt ctgcacgaac
    23401 aaactatgga cataacaatg catattatga aaaaaacaat tatcgtgaac aaactcgtga
    23461 acaaacaaaa aaacgcaaag aagcggaaaa aatcgatgat gacgaatgga gtgactttta
    23521 atgtcgtact ttcaacgtat tttagttaat acgttaacat tcatatcatt ggctgttctt
    23581 tttcctgcga tggtccatgt gagcagtatt tggacggccg ttcttgccag cttcgtattg
    23641 tctgtgttga atatgtttgt caaaccgatt ttgacgatac tgtccctgcc ctttacatta
    23701 gtcacattgg gattgtttac gtttgttatc aatgcagcga ttcttaaaat gacctccttt
    23761 tttgtgggag cagaacattt tagtttttct tcattcggtg cagcgttgtt agtagctatc
    23821 ttgatgtcgc ttgtcaatgt aattgtcagc gaacataaca gtagaaaata aaagaaagct
    23881 gtgccgaact atcggcgcag tttttttatt tagaatcagg aatgcgtttt ctattacatt
    23941 ggtaattaaa taaaaacgtt tttttta
//
LOCUS       DAJSMI010000024        23818 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJSMI010000024 DAJSMI010000000
VERSION     DAJSMI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.024"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            375..1814
                     /locus_tag="ON430_000812"
     CDS             375..1814
                     /locus_tag="ON430_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3218746.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2109..2327
                     /locus_tag="ON430_000813"
                     /pseudo
     CDS             2109..2327
                     /locus_tag="ON430_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2576..2791
                     /locus_tag="ON430_000814"
     CDS             2576..2791
                     /locus_tag="ON430_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218747.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3008..3264)
                     /locus_tag="ON430_000815"
                     /pseudo
     CDS             complement(3008..3264)
                     /locus_tag="ON430_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3451..4362)
                     /locus_tag="ON430_000816"
     CDS             complement(3451..4362)
                     /locus_tag="ON430_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3218748.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4381..5385)
                     /locus_tag="ON430_000817"
     CDS             complement(4381..5385)
                     /locus_tag="ON430_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR3218749.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5382..7505)
                     /locus_tag="ON430_000818"
     CDS             complement(5382..7505)
                     /locus_tag="ON430_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3218750.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7510..9957)
                     /locus_tag="ON430_000819"
     CDS             complement(7510..9957)
                     /locus_tag="ON430_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3218751.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9944..10333)
                     /locus_tag="ON430_000820"
     CDS             complement(9944..10333)
                     /locus_tag="ON430_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3218752.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10393..10896)
                     /locus_tag="ON430_000821"
     CDS             complement(10393..10896)
                     /locus_tag="ON430_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR3218753.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10909..11133)
                     /locus_tag="ON430_000822"
     CDS             complement(10909..11133)
                     /locus_tag="ON430_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218754.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(11237..13147)
                     /gene="ltrA"
                     /locus_tag="ON430_000823"
     CDS             complement(11237..13147)
                     /gene="ltrA"
                     /locus_tag="ON430_000823"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3218755.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(13893..14030)
                     /locus_tag="ON430_000824"
     CDS             complement(13893..14030)
                     /locus_tag="ON430_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR3218756.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(14027..15211)
                     /locus_tag="ON430_000825"
     CDS             complement(14027..15211)
                     /locus_tag="ON430_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3218757.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(15470..15760)
                     /locus_tag="ON430_000826"
     CDS             complement(15470..15760)
                     /locus_tag="ON430_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218758.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(15750..16892)
                     /gene="dcm"
                     /locus_tag="ON430_000827"
                     /pseudo
     CDS             complement(15750..16892)
                     /gene="dcm"
                     /locus_tag="ON430_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(16968..17132)
                     /locus_tag="ON430_000828"
     CDS             complement(16968..17132)
                     /locus_tag="ON430_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218759.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(17125..17799)
                     /locus_tag="ON430_000829"
     CDS             complement(17125..17799)
                     /locus_tag="ON430_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3218760.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(17877..19217)
                     /locus_tag="ON430_000830"
     CDS             complement(17877..19217)
                     /locus_tag="ON430_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR3218761.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(19300..19635)
                     /locus_tag="ON430_000831"
     CDS             complement(19300..19635)
                     /locus_tag="ON430_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCR3218762.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(19645..20019)
                     /locus_tag="ON430_000832"
     CDS             complement(19645..20019)
                     /locus_tag="ON430_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3218763.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(20032..20346)
                     /locus_tag="ON430_000833"
     CDS             complement(20032..20346)
                     /locus_tag="ON430_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3218764.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(20459..23686)
                     /gene="fss3"
                     /locus_tag="ON430_000834"
     CDS             complement(20459..23686)
                     /gene="fss3"
                     /locus_tag="ON430_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCR3218765.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         6663 a         5144 c         4108 g         7903 t
ORIGIN      
        1 tcctatggaa ttgaatatca tagcaatgta tttgaaaagc aaattggaaa acaaataggt
       61 gcaacaccat aaatatgaaa aaattgggaa aaagtagaat ggaaatttgc ttacgtctca
      121 cgagatagct cacactatta atctgaagaa caatgaagtc gacttttaga aattgctaga
      181 agaaagtaaa gcgaattatc actagttact atagagtaag ttcaaataaa gcatctccaa
      241 cttacatggt tacatgagga tatcaaatcg aaattactca agcttcctat ggctagagtc
      301 gcgagaagtt gttgggcagt agacataggt tatattgttc ttagctacac gatcattaga
      361 aaggaaatcc tattatgtat agttatcaag aagatgcaaa agtccaaaaa aatcgctggt
      421 ggattttgat tgctatatct atgtttacct tcatgtccac attggatggc agtattgtca
      481 atattgcact acctaccatc tcagatgata tgggcatacc aatgaatcaa gcggaatggg
      541 tagtgtccgt ttatctgatg attgtctgtt cttgtttgtt gctgtttggt aaaattggcg
      601 atagccgtgg gaaaatcaaa gtatttcgga ttggtacgct gatctttgtc ttgggatcgc
      661 tgttgtgtgg gttcaatcat tcgctaggta ttttattgtt cgcacgggtg gtacaagcca
      721 ttggtgctag tatgactatg gctacaaatg ccggaatcat cacggaagtc tttcccatac
      781 atgaacgtgg tcgggcattg ggagcaatag gtgcgtttgt ttcattaggc tcgattgctg
      841 gaccaggtct tggcggcttg attttgtccc aattttcttg ggcgtacatt ttttggatca
      901 atgtaccggt gggaatcgta acgatgttga ttgctgaaaa attcttacca aaagatatca
      961 ccatgagcgg ccatacaatc gatggagggg gctttgctgc cattgcggct acgattatca
     1021 gcttttttgg agcaatcttt ttggggcaag aaagtggctt tttccatcca gtcccccttg
     1081 tactgttcgt aatttcagtg attgcctttg tcagcttttt aaaagttgaa aagaaagcag
     1141 cctctccatt gatttctttc ggtattttta agaacaagat ttttaccatg agcttgatta
     1201 cagccttgtt gattttttct tccaacttct ttgtcaatgt agtgattcct ttttatctgc
     1261 aaaatgctcg cgggttgagt cctagctatg ctgggctgtt gatgatggtc tttcctttga
     1321 tgatggtcgt cggggcgcct ttaagcggct acctgaccga ccgggttggc ccggagattt
     1381 tggtattagt aggcttgggc ttattgtcgg taacccaggt catgtatatg tttttgtcag
     1441 tggactctcc tttgtggcta tacattgtcg ctactgccat catgggtgcc ggcaatgcct
     1501 tgttccagtc ccccaataat acgatggtta tgagctcggt cactatcgaa aacttgggac
     1561 tggcaggctc gatgaattcc tttgcccgaa atttggggat ggtcagcggg atttcgcttt
     1621 ctacgacggt cttatatcaa gcgatgtctg ctaagatggg ccagcaagta actaactata
     1681 tcgcaaatcg gccggatgtc tttttatatg gaatgaagat taccttttta ggcagctttg
     1741 ttttgtgttt tgttgccttt ttcctgacta tctggcgtat caaaaaacaa ggaggtattc
     1801 agaaaaatgt ctgaaaaaac cactgttgaa gaatttttgg aggcctgcgt atgggtgagt
     1861 gggatttcca tggatgatgg aagatgaagt gaacttctaa aagccgaaaa actcaagtat
     1921 tatcttcaaa atactagtgc tcaatgagat gcttgatttg atcacaagaa gactgcgaaa
     1981 gccgctggca accgcaagct aggtatccca ccgatggact acgttttaga ggctactggt
     2041 tatgaatacg gagccaacgc tctcatctgc ataagaatac atcacccaga ttatgtaaag
     2101 attttcgata cttctgtcaa aaaatcatta agatagataa atgatttccg tctagtcaaa
     2161 ttggttcaga atgtagacac acagatggta agaagtcact cacaattcga gaatggactt
     2221 gtcctatttg ccgtatacat cataatcgaa acgtcaacga tagtatcaat attttgacca
     2281 agggctatga atacaagcat tgccttagaa aacatcaaga accttaggaa caacggggat
     2341 agcttggtta ataagagata ccgctgttta ggcttgcaag tatactcttt tatcaagaat
     2401 tccgtggctt tagtcatgag aggcttaata ctatgaaaat tttctttagc atttttgatt
     2461 taggttcctt ttctaattat aataagatga ccactacagt aacctactga aatatataaa
     2521 cagccactgt agtaccattt tcaaagtagt ctatgagagg agaatcttca ttcttatgaa
     2581 cgctggaatt gcggatatga atttaataaa aaaaaccctt aatgatttta cttccaattc
     2641 aatatcaaaa ggtactggta tcaatttaag cacaataaaa aagctgaaaa gtggtgaaag
     2701 atcagtggaa aagctgaatc ttttagatgc tataaaaata acggaatttg ctatgaaaaa
     2761 tggaaaagct gagattgaaa tatggagata attatcgtct agtgaattac ttgccactta
     2821 gctgaattta acgatccttg gtgcttgaag ggggagattc ttgggaatag agcatacttg
     2881 caagcctatg tctttactaa caacgatatc tcttattaac caagctatcc ccgtagttct
     2941 aaggctttta cttttatcta agcaataatc gtattcaaag gaaatttttg acccttagaa
     3001 agtctcattt tatcagacgt gagcttaggc attgctgaat tttgttaatt cggttttagg
     3061 ttgtgctttg agcataatat gaaagtattc tttatcacga tttcattcat ctaaggtaat
     3121 gtgatgtgat tctgaaattc tttcaaaggc agtttttgca taagctgaca ttacatcatc
     3181 aatgacatgt ttacaatgtt tcactactaa aataaggggg taaccaagaa gaaaaactga
     3241 atgattattt gtatctaatt ccatggtata tcaaccattt cctatataga ctgattatga
     3301 catggattaa aataaaaagg cactatgtct taattgtcgg ttttcgagtt accgaatggc
     3361 attcatcacc cacctataga ggatgggcga cttctgcctg aaataagtta cactacttat
     3421 aacttctaat aaaattagat tgagtcatta ttatttaaca tcaacgattt tccagtttcc
     3481 atttttttgt aatactaatt tgtactgaaa gtagctagtg gtttttgata cattatcaag
     3541 gtattttatc tttgtactca ctagaaaatt ttttccgttt tgtagataaa ctggattctc
     3601 taactccgaa aactttaagt caatattgat tggtcgcatc gcaccatttt caacataata
     3661 ctccaattct ttttcagaag ctgatggata aagtttaaaa aatgtttcta aaaaatcggt
     3721 gatttcttcc gttgttttgg catcaatttc tgaatcgttt tctacttgtt ttgcttcata
     3781 ttttgccttg tctggctttc caatcattgt tggcaaacta gttactaaat gattcctgtt
     3841 ttcatcttca aagattgtca ctcgatatgc agaagaaatt gattgcgtct tttttccctc
     3901 tgtaatatgt tgctccacta aaaatgaaac gacaaattct tccgagtgtt tttcaacatc
     3961 tagaattttt acagattgaa cttcggaact cgtgggaata tctgctcgaa ccatatcttg
     4021 gattaaagcg agaccgtttt ctgtaaggta ctgttcgaga ttactcattc tcttttcaat
     4081 tgcttctttg ttatttttcc atgaaaaata ttctttcgca aaatcctctg taaaactttc
     4141 aacgccagaa gtattcacta ccttctcttt tataaccact ttttcatgag ttgtatgttg
     4201 atcaatggcg gtgaaatttt tataaacgcc aaaagcaaga ctacaagaca ataaaagcca
     4261 aagggcaagc accatttttc gatgcttacc cacggataca actcgttctt ttttcttttt
     4321 tgggctcttt tccttttgat tccgttcaat ttttatcttc attctttttc ctcttttctc
     4381 ttatttgtat cggccagctc cgataagatg ctgccgccaa tagttttccg ttagattcgt
     4441 ccaccccact ggatctcctg catgaaacat gcgattctcc cctgcgtaaa tgcccacatg
     4501 ggtcacataa gtaccggcat cataggttga atgaaagaaa actaaatcac ctggtttggc
     4561 atcttttaga tcaatatgag tcatggcatc atattgatcc tgagccaccc gaggaagttt
     4621 caggtcaact ttggcataac accattgggt caagccacta caatcaaacc ccgtttctgg
     4681 acttgagcca ccaaacacat aggccgttcc ttcatactta aatgcctcat caaaaatttc
     4741 ttggactatt ttgtcatcaa actgagttgt agttaggtat tgcttcacta ataaacagta
     4801 aaacatattg ccataattat accgccaccc accattttcc ttaatcgcga ttgggttggg
     4861 gtaatcgact ttttgaccac ctgatttttc ctttgaaaaa gattcagcta aagcaaaact
     4921 atgcttcttc ccgtttttcc cgacatgatc tagatacccg ccaccatagt tataggcctg
     4981 aataatacta tcttgatcac accctagttc tttggaacgc ttaaccaaag acgcaaaata
     5041 gagacagcct tgtttgatgg atgcctcggt acttaatgaa ttaggtggta atccggctga
     5101 ctcagaactt tgcataacgt cttccattgt gccaccagat tccacttgaa taatcgccaa
     5161 aagaattgga atatactctg ttatatccga ttctttggca tacttttcca ccattgcacg
     5221 gtgtttcaat acttcttctg agacactagt tccgccagga gaagtgccag aactaccatt
     5281 tccctcactg tcttcaccaa acaacaaacc cacacacatc aaaataccca taaaagagat
     5341 ggtcagaaaa gttaatgaaa taacacccac tttcttcaag ttcatttttt ctcacctgac
     5401 cttgattttg gacgatggtt cattcttttt tgtacttttt gattggtctg ctttggcaga
     5461 gtggacctct gtttcacttg ttggaaagaa tttttcttac ttgctgattg gcttttccct
     5521 tttgaaatga tttcttgact agttagtttt cgtttgattt cgggattcag atttgctctc
     5581 acaatccgct ttggtgcttc ttttttcagc atcatatctt tctgaggttg agctgcttta
     5641 tctattggaa tattcgccct tctttttccc tgacgatttg ttgcttcact cagtatcttg
     5701 cgtttttcgt ccattttcgc atttcttcgt tgtcgttgtt tttctcgcaa atccactcga
     5761 tccgcttttt cttggaacag tccgcgcttg aattcttctg gtgctttttt cgacttttcg
     5821 attcctttat ggagattgta tttcaaattc gttggcgtat tccttacctg atcctttact
     5881 tgtttagtct tatctttcac caaagcttgg gtatccaaaa ctttgccgat ttttctccca
     5941 gccaaattca ttcgggattg tttcttatta tttgaaactg gattctcctt agttaagtca
     6001 gatagtattt cgtgatcgtt cggtaatcgt gaattctttc gtagagaaga accggatggc
     6061 ttaaatttgg attttgattg ttcctttgcc aacttatttc cgactgctgc accaccacca
     6121 aaagccaaag ctcgtcccac atttcgattg agttgccgca attttcgatt cattaacatt
     6181 tgcggttttc gcatgacgcg tcttcccatt gactggctat cattactctg gaggttgaac
     6241 atactcataa tgtcgcccaa tttgaaatag ataccggcaa aggtcacaat ttgcagaaaa
     6301 gcaaccatga aaaatggata ggttgccgaa atattgaaaa acatcgtgga gatgctaaat
     6361 gcagtcgtaa tcaccaaagt aacacccgct cgcatcataa tcgtattgaa taaacgaata
     6421 atcgccttct ttcctaaact ttcataagtc ggcaacatgg acaataagaa actaattggc
     6481 agaaacatcg caaagataat gaagagaatt tgcgagaaca acataatccc tgttagaaga
     6541 aaaacaaaga aggaaatgcc aatattgaac aataccaaaa agaccacggt ccctaaacgg
     6601 ttcatggtct tggtaatact gagattcgca ttgtcattgt cttcaatctc tgccttgacc
     6661 gcttcttccc gatctttgcc tttgttttca tctggactca cagatagaat cttgttgacc
     6721 cggtcttcac caatttcttc tatgttggaa tcatcaaatt gcagtagtaa ccaaggttgt
     6781 tggacttgaa tcgaaaacaa actatcccga atcaaatcca cactatcttt tccttggctt
     6841 tctgaattgg gtacaacgat ctcggttcca agggtaaggg ccgcttcact aacatctgag
     6901 ctaaagtcat tgatttttgt gatataggta ggcgcataag caatgaacga acccgatagt
     6961 agaaaaatca tcacaaaatt aatcacggct cgaactgctt tcgtggtttc tcgttttaat
     7021 aatccggtat acgccacata cacccccaga gctaaaatca tcaataatag aaacccaaaa
     7081 taaaatccgg aggtctggag tccattttca gtaataccag ccaatgtttg gatattcttt
     7141 ccaatacttt cagccgtatc cgaaatgaaa tctaatttgt aagcctcttg aaccacataa
     7201 cccgtggcgt tcgacaaata caaactcacc gtccaaatga aattagtaat ggcataaaga
     7261 ccatacatca cgcttttccc taagccatca ccccaattcc aaggcaacca atcccaagag
     7321 ctatccacaa agaaatctag ttgatagttg ttcagtgaat atttggaata tagatttcct
     7381 gcttcaactg tatcatcaac tagccctgtc gcctcggcta ccgtaccaac taaactcaat
     7441 agaactatca gagtgagtat gctaataaaa acgattccca caatccgaaa aagtttcttt
     7501 ttcatcccct cactccattc tctcttgaac aggtggacgg gtatcaaagg catggaagag
     7561 ttcttcaaag actggatgta cctgaaccac gcctacccga ccatacaaat cctgcatcaa
     7621 gcattggcca ttttccaaat ctcgtaggcg tttttgattg ctttcatctt ctcgatccaa
     7681 gccaaagaat tccaaggttt tcttgatttc atgaatatcg gtactgcgaa acgcaaactt
     7741 caacccgata ttattcttca agcgttcatc cagtaaatca tcactatttt gcgtgacaaa
     7801 gtaaaccccg gcattcatag cccgcccagc acgaacgagc ttattcgata atgttttccc
     7861 ttgcgctact tgaagaaaac tccacgcctc atctaagtct acaattttga agatttctcg
     7921 attactatgg ataaagtcca aggcaaaagt ggaaatgacg attagcatgg aaacactaag
     7981 caattccatg gtggtatatt cttcaaaagt ggtttctgca tctggtaaaa ccaaatccgc
     8041 tacttgaatg atatttaact gcctatctaa actaatcgac tgggtaatcg aaccatctga
     8101 gaaaagcaaa tgggcaaaat cataatcggt aaaggactcg atgtgatccg cgatattttc
     8161 cgcaactggt gagccttctt ttcgaagctc attaattact tgtaataatc cccgttgatt
     8221 gctttgggta accgtccgaa tggccttacg caacactgga aacttctccc catctcgact
     8281 agaaattcct gttaaaaagg tcagaatatc aattgctaga ctttcagaat ccttcttttt
     8341 ctccataatc acataaggat caagtaatcc ttgatttttt ggcttactag ttaagttgag
     8401 aatattgatt tcatccgcaa tttctggcaa ggtttctttc cagccacccc gttccccttt
     8461 tggatcgaca atcacagctt gtccaccaaa aaggacggcg taataaacta agagattgtt
     8521 actaaaggac tttccgccac cgagtgaacc gaggaatgcc gctgctagcg cattggtaac
     8581 tgaacccttg accccttgtg aggccaatgc cggtttcagg taaacattgc gccccgtatc
     8641 tacgttataa ccaaagtaaa tgccttccaa ctcccctaac atctgagtgg cgccaaaacc
     8701 aagtccggcg agaaaatcag acgtaacata ctggatataa tcattcatgt atcgcttact
     8761 agaaggtaag aattcttcat gtagccccat catatcgcca aaaggccgga ctagttttac
     8821 attggtatca tcataaaaat ctagcacttc atcacaccgg cgctttagtt catctactga
     8881 ttcggcactt actcgaacca catagctcag tttgtacatg gattctttgg actgatccaa
     8941 ggtagtttcc aattcatcta cggaatccaa tgcatctaag acattggaat ttgtttcatt
     9001 gtcagattga taggcatggt tgtccaagtc ttttaattcc tttttcttat tgcgaacggt
     9061 agcaagtgcc tttttgttcg tcacaatctc cacattcata ctagtgtcaa ttgggaatgt
     9121 aaattgttgt tgctggtaat agaaaagttc tgaggaggga aactccattt cccccacaat
     9181 tgtattaatt gttaagtacg ctacatatga ttcatgattc tcatgttcca tgcataagta
     9241 ccggggcttt tcttcaatca aacaacgact gggacgtaat aagtcgtaac gtttcaccaa
     9301 agtttccgac ttcagctttt tctttggtag ctgaaattca tattcttcaa atggtgtacc
     9361 cttttcgcca tagatatgtt caatcaaata catcagatca ctcggtgtga ctcgtctgac
     9421 tttaaatctt cgagccaatt tactttccat tagtttttca agcttggtat atcggcgaat
     9481 ttcttcattg gataaggaaa caaaatcgcc catcaaatga tgattcactc catagacaaa
     9541 ttcttgaaag ccggagaaga aagatttctt gagattcttt agattgacct cttcatctgt
     9601 cgcaatcagt ttgaagccaa taaaaaagcg ataatcaatc tgactatcgc caatcatgga
     9661 tactaatgcc tctgtctgta aatcaattcg ttgtttcgct acttctgtta atcgcccggt
     9721 aatttccttt tttgatcgtt cttgcgtggc acgaatacta ctttctgtgg caatttgtaa
     9781 ggcatgaatc ttgccttcac gattttgcgc aatcaattga cgaaagttat cgtggacttg
     9841 atatttctgt tcaggtgaca aaaaggagta attgtaaggt attaactcat agtaggcaaa
     9901 acattctccc tcttgattaa agacaagatt attttcaata tacttaatcg ggtacatagt
     9961 tcacactcct taccagagta atgggttcgt cccatcgttt cttctgttct ttgactttct
    10021 ttcctgcgaa agtgacctta aatcgtaagt gatagctaat aaaagatcgt aaaaatccca
    10081 atggtttctt accgtcaaac gttttttgcg acatgaacca agtaactgcc accggtatac
    10141 ctaagtattt taaaaaggcc ccctctatca tagatagtgg aggcacatgg gcaaatacga
    10201 tgacaaacag caaggataaa acaaaccaag ccatctggtt aaaagtcaca ggaaaaggca
    10261 gttgaaagtc attaattgca taaatgacct tttcaactgc ccaaatactg gtgtagcttt
    10321 ttattttctt catcaaatca ttcctttaaa aagaaggata gtttagaaat caactaccct
    10381 tcaataattt tgttaatatg gatactcaaa aacacctcga ctcgttacca aataattgcc
    10441 tccaatctcc aagtctctac cacacgcatc aaaatcaata tacgtcttca agtgttctgg
    10501 tacttctcca aaatcaccag cttctacaat caagaaatat gctacatcac tcatatcatc
    10561 gcaatctgga taatagataa tatcatccac atgctctacc atctcctcaa aagaattgaa
    10621 gaaagcatgt tgaatctcag aagcaacttc accaactggc gttccttcaa gttcctccgc
    10681 taagccacac aaccgattaa tttcctcaat tggcgtatac tcatcaatct caaagggaag
    10741 ttcataatca tgaattgcat attcctcgta ttcgtcattt aacccaattc gttctttcac
    10801 ttcatcaaaa tcgacaggtg gggtaaacca cgcccccacc aattcacctt cgttatattt
    10861 cccgaggttc gcaatataaa cacgcatctg ttccatcgtg cttccccctt acgcaccgac
    10921 aattcggtta aacaaactga gtaagacatc cttcacccca gaagcattaa agaccaatcc
    10981 gaccgcaatc aaagcaatca ccaaaaagcc aatcagtttg gaaaactccc gcttaaatcc
    11041 taaaaacaaa ccaatgacca caattgccag taagactaag gattgggcat tggttaaaaa
    11101 ccattgatat atggataggt aagttcctga tattataaat atctcaaact tttcccccca
    11161 ttcacacgga gcgtgcgact ttcaccgcac tccgcgttcc atcgtgttta tattgaaaag
    11221 tatttatgta gtagacctat atcaagttta attcttgatt gccagctaat actctatatt
    11281 tatttagtct tatgagctgg actttattta gcttcaatag agctatatat ttttgtattg
    11341 tttcttctgt tttagcgtgt attagacggt gtaccgcctc ttttaccaaa ataagatttt
    11401 tataatcgtc atttccacca gctccagttg gtacaatatg atgacaatgt atttcattag
    11461 cttgttgaaa ttcttccaga gttatagcac attttccatg ttgagcagaa aatagcgaaa
    11521 ttcgattgtc tgcgtattct attgtgcgat tttccaaaga taatttcatt aactggtaca
    11581 tcagtaattt atcaacactt agattggtgt ggataaattt tcttccctct agagtgtagc
    11641 aattcgctaa atacaccttt gccattggtt ttctgtgttg gatatatcca acagggaaaa
    11701 taggttcgcc ctcgccagca acataccgaa gcattttaga tttaccatat cttgttgcct
    11761 cctttgtcgt aagttgtctg cctgtttttc tcaatctgcc ccttttgttt gtacctaatc
    11821 tgttagtgaa gatagccatt acagctctat tcagtttcat acaatccaga tttacattcg
    11881 ttgcaatctg ataataattt tgcataccta ataccattga attataaagt cttgtttctt
    11941 ctaattccgt tttccctttt gagggagaag caattttctt tgcctgtgat accagcttgt
    12001 ctttttggtt tttgaattgt ttatcactta tatgtgattt aacaactaac ttctttcttc
    12061 tcggtatcat tttaatcttg aaaccaagaa agtcggagta tctttttcta acatttacaa
    12121 ttcttgtttt ttcttgtgat acttccaatt ttaatcgttg ttcaatccat tgagtgattg
    12181 ctattttggc tttttcagca ctttccttgt agcggcagaa aactctaaaa tcatctgcat
    12241 aacgaaccat atacatttcc tttaactttg ttttcttcat ttctttgtaa gcgttgcttt
    12301 tacatgggct accgctttta ttgatatgaa ccacatagtt ttttgtaaca ggattttcct
    12361 gccattggct ttctacccaa tggtctaact cattaagaac aatgtttgct agtagtgggg
    12421 atattattcc cccttgcgga gttcctttct ctggatatgt tattgtaccg ttagtcattt
    12481 tgattggagc ttttaatatc ctttttaaaa tgaatatcaa gtgcttgtcc cgtattccca
    12541 tagcccatat ctgttttatc agcttggaat ggttcacgtt gtcaaagaaa cccttaatat
    12601 caaattcaat cacataatgt agctggctga tttgtaggcg gttataggtt gcttttattg
    12661 cattttcaac tgacctatta ggtctaaaac catagctgtt ttcactaaat ttagcttcgc
    12721 agattggttc taatacttgt tttatacatt gttgtataag cctgtcccac atacaaggaa
    12781 tacctagagg tctggtttta ccatttggtt ttggtatttc ttttcgcctc actggcttag
    12841 gtcgataccc atgtggactg cctgcaacaa tgtatcttac ttttttgaca actgtttctg
    12901 ctggcaattt gccaatatct ttaattattg taccgtctgt tcctgctgtt ttactgcctg
    12961 catttgcttt gatatttcga tatgccagta atatattttc tctcgatagg ataatatcca
    13021 taagatgagt gaaaatatct ccattcttac ttttggcata taaatcatca aaggtgtttt
    13081 gcatttcgta atattcagcg tgtcttaaat cttctacaca caatgtttca tttttatttt
    13141 taggcataag gcttcactcc tttacttgaa attgagtgag cttttttatc ttactcgtaa
    13201 tccttattga aacaacataa tacttttcta atatatacac gacttgaggg cattcctcca
    13261 ttctcattac aagaatttct taggttcgcc ctctgctttt cagcattagt tcaactgctt
    13321 attgttcttc gtcagtttat tctcttgatg agaacccaat acctttcctc gttccgataa
    13381 tcttatctat acatatatat ccttaggtca ctagctataa gcctgctggc ttgataccgc
    13441 ctgtaacgtt atatggcatt tcataaccta tattcttact ttgccacacc agctacgatt
    13501 tctcgtacct ttgcatttct acaaagttca aatttagacc cgtacattcg ctgtttcgtc
    13561 agttccttat agaacattct aaccatagat attttataga cccccgacct gttggatacg
    13621 ctacccacct ctggatagtt ttcgatagaa gatatatgtt catcaactat tacggtatct
    13681 ttcagccgtc tttaccgagc ttcaaacatt ctgagtttcc tcatacaatg cttgtcggag
    13741 gctcagggca ggcgtttcag agcgttactt catcattcca cctgtcagat tatcagttct
    13801 cctaataacc aaactttttc gttcttttat tagtgcctta gcttttcact aaggaacgag
    13861 tcgcacataa attttgtccg aaattcatct tgtcactttc ctttcttttc cattgctcga
    13921 tccgctgctg cacttgcatt catcaagcac atcacagtaa caccaagcat actgccacca
    13981 aataccaaaa aaatatcttt tacaattccc atgacgattc ctcttttcat ataatcaaat
    14041 tttcaattgc catactttgt tgctccaaaa tctttttatg tcggtctgat aagcgtgcat
    14101 tctgtaccat atcctggata taggttgtcc gattcaatcg atccaactcc ttagccattt
    14161 tccacgtagg ggcgacttgt cggcctagcc aattcaaggt tcgttcaatg gtatacggtt
    14221 ttggctcgga agttaattga atctctttcc gattccgtcc aataaaacgc ccccattgtt
    14281 cattcaaggg ccagttggtt cgccttttac cttctacttt atccgcaaat cgcaagtaac
    14341 gattgataat ggaaaacgtg gtgctctcaa tgctacgacc ttttaacaaa tcttgaatcg
    14401 catgaaaagc acggtcattt ttcagtcgaa tctcaaaacg atttttcgtc tcagtttggt
    14461 ctaatggaat ccctaacttg atatattgtt catagtcttt ttcatacaaa cagaaataaa
    14521 cctcactctt gagactgccg atatataagg tattgcccat gccgtccttt tcatcagcct
    14581 ttaaaagttc cccggaacga taacttttaa aggtacgaaa caaggagata cattcttcct
    14641 tctgacattt tttcgttagc tcaggtatat ccaatagccc cactcgatca ttgattgcca
    14701 agtccaagcg tttcatcacg ccaccggcct ttagacaatc ctcgaaaaag tcgtaccagc
    14761 tacgcttttg agcgagtaaa aaagtttcaa attgtcggca acctcgcccc tttaattcta
    14821 aaagcacacc tttatcttct tcatgagaca acatcaccac aatatcaccc atgacatact
    14881 gttcttgata ggagtaaaag gcataatctt catggagcat atactcaaat ttcaaatgga
    14941 gaatttcttg aataattttt cgtgcatccg ttgtgggaaa gcgaatcctt acatagtcaa
    15001 acagtaacgt caaaggcaaa tcgggattaa agctttccag ttggtcaaaa attttcctca
    15061 tggtttttat agatgcggtt acgccaccat tttctatctg atttaagtac ggccgactaa
    15121 gtccacaagc cgtagccaat ttattttgtg aaacgccgta ttcgttgcgt cggtctttta
    15181 atagccgata atctaaattc cgttgtgcca agttctcacc tccaaattca aggtgttaac
    15241 tttttcaaat gagttaacac ctatacaaat tacttactat ctttgataca acaagcattc
    15301 ttaatggttg cttacttatt ttttgtgttt ttgtaccccc ctgttagata cgggggtgag
    15361 acatcatggt cggctaacgc cgtccatgaa caggcacagc accacggagg atttctgctt
    15421 cgcgaatcct ccgcggtcag cgcctaacag tcagttgtgt tggtttaggt tactcatctg
    15481 taataccatt tacttccaac tctgctttca tttctaacaa gtattgacga atatctggtt
    15541 cttttgcttg gtccgcaatc cgctcaaatt ctgcaattac ttgatttatt tcaattcgag
    15601 catccgtctc ctcattcatt aaatatggat cgccagcgac tttgacacca ttcttgcgaa
    15661 tatcaaattt taaccgtcgc aattgttctt ctaattctgg gtctgactga atctttccaa
    15721 tcacccaata aatttctcca atcaactcat cataaaccat gtacatccaa ctcttttaat
    15781 cttctggcaa tatcaaaaat gaccggaact gttacagagt ttccggcttg cttatacaac
    15841 tgactatctg aattgacttg agaatctcga tcaaaagccc aatcgggaaa gccctgaagt
    15901 cgccaacact ctctaggtgt tagcttccga atttgatact gatccgtgac gatcgcttgt
    15961 tggtctcctg ttagtaaggt atgaacactc tgttttccca gacgacctcg gcgacttgtg
    16021 gaattaggaa aagccagatt cacactgtct tctggcttca cctcttgata ttctgtttca
    16081 ttttttcgaa tcatgatttt tgtaccacct tgtttcgttg acaaggtggg ggcggttcca
    16141 gtaacttcat aaactcgatt accttgttca aactttcccg gtaatagtcc tgctacagag
    16201 attccatccc ttgctggcag tgcgattttg attccttcat cacctgttaa ggttggcgct
    16261 acgccatctg aaccatagac ttgtccaccc attcctcgat tggaaggatt gatatttacc
    16321 aaatttctga tgttttgatc agatgttgaa gacggtccaa tgataggaaa tacttgttcg
    16381 gtacatgcgt ctcgagaatg tccgataagg aatatccgct cccggctttg gggaatgtag
    16441 gcctttgagt taagcaccta ccactccacg tcatacccca atccatccag cgttcggagg
    16501 atcgtctcga acgtagcccc tccttcgtga ttgagcaatc ctcggacgtt ctcaaggaat
    16561 aaaagcttag gtcggagaag agtggtgaac cgagcgattt caaagaataa agtacctcga
    16621 gtatcggaaa atctttgccg ctttcccgca attgaaaaag cttggcacgg aaatccgccg
    16681 caaaggatgt ccactggtcc gagggattga ataaactcat ctgatacact tgtgatgtca
    16741 tgcatttcca cctccttact tgtatcatga attgctttat aactacgtct agcaaattta
    16801 tcaatttcac aaaaaccgat acaatgatgg ctcgcctgtt ccatacctaa acgaaagcca
    16861 ccaataccgg cgaataaatc taaaaatctc atacattacc tcattcattg caaagaaaaa
    16921 agccaagaac attttgctct tggctcatac attattaatt tgtcctacta ttttttattt
    16981 aatccaacta atttccgttg tttttcattg gcctgatcgg ctacatattt aatcaccgtt
    17041 ttgaactctt ttgcagtagc attttccgat aaatccgcaa tgctcttatt gtatttcttt
    17101 acaattttcg ttacttgttc tttctcagtc atgtaattga acctccaaat ctttcctact
    17161 ataccctagt ggtaaaaata attctagatt atccgtagca taatgaactt cggaaatatt
    17221 attcgtataa tcttttatta tgatatgaag caatacatct ggatgtttac tctttacttt
    17281 tgcaatgttc tctcttgatt tgaaaaaagc gttaataaat gcctttttcg gcacatgacg
    17341 tccctctgca atttcacgtt cttgtgtaaa tttccaagcg attttagggt cttgataaac
    17401 atagaaaatc gtcactcgaa aatgtttttt caacgcccgc gagatatttt tttcagcact
    17461 taatattgca aaagttgcat ccagaataaa agaatagcca tgatctaaga catatctaaa
    17521 tgcctgctct accaaccaag aagatgcttt ttgaaagaga ctagagtttt ttccattata
    17581 atcaggaaac tgggtgcgaa agtaatccgc atcaataatc acatggttat cataattctc
    17641 agctaatcca atggcaacct ctgattttcc agccccagga ctccctgcca taaaaactgc
    17701 gtctcgtcca acttcggggg atttcccttt gataatataa gaaataaact tatccttatt
    17761 tgcttttgca tactctaaat aagaatcatt actttccata ttaattatcc cctttcttcc
    17821 tactatataa aagtattata cgttacttga agtataaagt cagtattgaa gatcactcaa
    17881 tcattcatct tttctaattt actaattgaa tctaagaaat catacccttt tggtacaagt
    17941 ggcgtataaa actcactaat cacactgcca cctgtatcca cataacctcg acctttgatg
    18001 tgcttcataa agaagttttt ttcgacctcc ccaaacatca tggaatatcc gagttcactc
    18061 atacgaccca aagccacgcg aaagttgaat tgatcgcgga ttccgtctcc taaatatttc
    18121 gcatccggcc tttggcaagc caaaatcaga aagaaaccag attgacgacc caacatcacg
    18181 atttgtttga gcttattcaa aatcaccgca ctttctttcg tcgttaacat ctccatataa
    18241 gccacgtatt catcaaagat taaaaagttg ggtggaagtc caagataggc ataattctct
    18301 cctgttttgt agttagacat ttctttcatt gccttgctac gagccatcat acgttcatag
    18361 aaatcttcca cacaagcaga aatttcttcc ttttgtgaat aaacatgagg catcaccgta
    18421 cctaaatcag ctaaatctgc atttttggga tcgaggataa acaattctgc atctgacttc
    18481 aatagtgctt cgataatggt gaggaggaaa taggtcttcc ctccacccgt gccaccagcg
    18541 attaacatat gtggtaaaga atcataagcc catacttgat ttttcatcaa tcgcagagtc
    18601 ccattttctg ccactacttc gtctatccca attcgattgg cgatcatatc atacagtaaa
    18661 gtatactcca cataggagtc tttgagttcc ttttctacta actcacaata cagtccgctt
    18721 tctaacttct tttccaattt taaaagctga tcttggtatt tcccaagtga aatttgaacg
    18781 cgaatagaca gtaatccatc tttcattcga taatagattt tggggaagta actgatagtc
    18841 tctctggttc ggctactatt caaatctttg aaaaagcctt cactttgagt ttgtttcact
    18901 tcataccaat ggttctctag aatcatccga gctagctttt gtcgatgcca caagcttcgc
    18961 caatgatcaa gaaaaaagtg aatataaagc aatatcatag ctcctgaaaa agcgagactt
    19021 ccaagcagac atactaaatc ttttatagac caaacgattt caatagtaga aagtgatagc
    19081 ttagaaatat caaattgtct aaactcttga aaataaaaga cgctcatcca accgatgaac
    19141 agagcgagta gccagccaag aacaaagtga taaaccaagt gctgatctcc tgcgcgtatg
    19201 cgatgtcctt tgtacatttt gatggtttcc acctccaatc gaaaaagccc aataggattt
    19261 cattaggcaa tttcaaaaag ttatgctagt catttactgc tacgaggagg ggagttgttt
    19321 cgtttcaacc aattgattta aaaagccttc aatagcatcc ggcatctcca caccaatttc
    19381 ttccaaaatg gattctgcct gccgacataa gttttcattt acttcgacaa ctataaagaa
    19441 agactcttct tctcctaacc agtctagtac atcagagtct tcttcactgt cgctattttc
    19501 tccaagtaaa ccttgatgta taatcagaaa cattttttca tcagaggtta atgctttaac
    19561 gagttgttcc tcagtcagct cgccttttcc atattgatcg agacaatcta aaatatgttc
    19621 aacattaatt tccataatct tcacctattt ctttctttca ttttggttcg taggatttcc
    19681 ttgattcggc tgccgcttca agactaaatc atccgcctta atgaaccaat taacggtagt
    19741 tccttctcga aaaacataat tggcaacggt atccacgatt ggattgacca actctacctc
    19801 tgcatcataa gcaaactcgc gtaagggtac attagctggg atactaactt gaatcattcg
    19861 cccttgtgca gaggatttta aattataggt tcgttctttt acctcattac caattgttcc
    19921 atcctcattt tgcttccgta cttctcggcg taatgcggaa aaacgcaact tcccaaatgt
    19981 taaacctgaa tctacgacaa taccttctgt taatctcatc ttcttttcct cctatgcttt
    20041 cactaaatca tcaacataca acacgtaatc agtaaaccca cgattctcaa tctcatagcc
    20101 tttggcaacc aaatggggat tcacggcttt tagcggttgg tcttgatcaa aatccttttc
    20161 accggcttcc gcagctacta ccacctcaat atcatcggcc cgttgcttac tggaatacaa
    20221 atgataactg cgaccaatca ccgtagtatt ccgtccatat ccttctgtta taatttctcc
    20281 ttcaccggca taacttaact ttccaaaggt ttcagccata tctggcacga caaatttcaa
    20341 ttccatcttc ttttcctctt ttcattgtta gttttggata caaaaatagc gcgactactt
    20401 ttttaggagg cggtcgcgca cgttggttct taggatttac ttgtatcatc tcatcacctt
    20461 agttccggcg attccagaaa taatagcccg ccgtcgcaaa aatcaaagta aaaccaatga
    20521 acagtaaaac attggaattt ttctccccgg tttgagggaa ggtcttcact gtgggactag
    20581 attcttgaga gccacttgga acagttggtg tttccggttg tttcggcgta cttagtatgg
    20641 ttggaacttc ttttggtgtt aaggtttgag atttttcttt caaatcttca ttgtgttttc
    20701 cattgatatt cccatcttta tcgtaattga tttccgcaaa agtaaacttg gttccttctg
    20761 gatattttcc ggtatctact tttttcgcaa gcacagtttt ggtgaattct ttatcattta
    20821 cttcataatc aatcttgcca gatttccaaa tttctttgtt tgtaccatcc ggtaataaag
    20881 catacagaat tgtttcgaaa gcttcttttg agccatccag tacatcatga gtaatcgaca
    20941 catcatcaaa catatccacc acatcaccat gagtaaaggt ctgagaaccg ttttctaggt
    21001 gggcctttgt ttggatagaa acatggtgtt ccactgtaca actgacagtt tgggcttggt
    21061 tattcaagct aacatccttc gctactggct tatcgcctgc ttcaaaagct tccttatttt
    21121 catacacata gttgaataag acaatagttt tatttaaaac ttgttcggcg gttaatttat
    21181 gctgaatttt ccaagttcct gttttctctt tgttcgccaa cgttgcgctt gtttctgcaa
    21241 tcaccactgg tttggctttt tcatcttttt cttgggcagc ttttgtcgca tccacatcaa
    21301 tggcttgcgc taccacatac cagtcttctt tgatttcatg tagattgtaa ctcaatttat
    21361 caactagctc ggtagaatcc agagaagtta attctttgtt tccgtctttc caagtcgcaa
    21421 gagaagttaa actatcttct tcctcaggca aatcatagag cattaaacgg ttcaaactaa
    21481 cagaaaatgc tttgttcgtc agcttctcgt aaggaacggt caccgtttta attggttgtt
    21541 tttggtcttg ttcggtaatg gtaaagacat actcactctt cacaaagtct tctttgtttt
    21601 ccttaaaagt agaacggatt tctaatggcg taatcttttg aaaaccttct ggggcttcta
    21661 cttcttcaag taaataatcc ccataaggaa gattttcaaa cttgccataa ccatcaaacc
    21721 ctaattgctc gttataagct gtggtcgctt catcttcggc acctgtgatt tcattggtcc
    21781 cttccaatgg cgacacttta aaggtcagat cattgaagcc agtttcggta gtgcccgcag
    21841 ctgatccagc aaatttaaag aaatcaaagc caaagcgaat cacttgttct tttgccgtaa
    21901 catttcgggt aatcacggca tttttttcgc tatcatccac ctttttgatg gatacaggat
    21961 actttgtttc atcaaggcta tatccttcgg gcgcttttgt ttcttgccaa taatactcgt
    22021 tgatagctaa atgtttaacg gcaacttggt tcttttcgtc taaagtcaaa gtgaccgttt
    22081 catcagaacc tttcgttccc ttcgctaact ctggtttaaa agcctcactc catttaacag
    22141 cttttccatc cttagcagta aacaaggtgt attcagcacc ctcaaagact gccttacctt
    22201 gtgctttatc cccagtatct ttgtcttctt tcgtcaaagt ggtttcgcca gtgacttctt
    22261 gattttgccc ttttacgtta ctggtaacaa gggctacggt ttgattggca tacttcaatt
    22321 cgacttttac aggtttgaac gtattcacga acccatgact agctttagtt tcggtcacat
    22381 aataggttcc caattccaac gcattagcaa tctcttttgg tgtttccgca tgaccgtttt
    22441 catccgtggt catttcttga acgatttcac cggttggact gtctttgcga atggcaaacg
    22501 tattaccagc taaggaataa ttgtcattcc aaagatcact ccctgtttca actccgcttt
    22561 tatccagaat gatttgccct ttttcccgtg cgttttttga agtcacgtaa atggtttcgc
    22621 ctgctttaat ggtggtagtc atgggggtgg tatcaatcgt ataaggcgcc ggtactgatt
    22681 tttcggtaat ggttactttt gtaccatgag gaatctcatc caatgtagca atgccttctt
    22741 tgtcagtagt cacgtctttt gcaggtaaag tttttccaaa gtctaaatgg aaaaccgttc
    22801 ctggtacaac atcacccgat tctttatcga cttttttgat ctttaaagaa ccctccgttt
    22861 ccacatcaat tttgattgtg taggtattag gtttatcaat cgccccggcc attaaagtct
    22921 gttggccagc ctttttatag gcgacaggtg ttccggtacc agcagatttc ttaagcgtca
    22981 gcacaccact ttcttttgaa ttggcatttg gggtgattat caactgatta ccattcacac
    23041 gataatcaat gtttgcggca ttttccacaa cctcatcgaa ctctgacaaa ttcagattat
    23101 tcgtatccgt cacagtagtc gattgcccta atactgtttt agccgtacta ttatggaagg
    23161 ttggtttctt ctgataatcg tcaatggctt gatttatttt tgcctcaatg gctgcgatat
    23221 ttacggcact accatctgat cgttttattg agtgaagggt atacccatta acttcttgcc
    23281 agatcatctt ctgtgccaca atatgagtat ctgcatctgt acctactttt ttccataaaa
    23341 cggaaactaa tttggcttta tcggacatat ctggtaacgg gtttttctca tagccaggag
    23401 tgaactcatt gtaaatcggg actcctggtt caatacagaa aatatcttgt ttaaagccgt
    23461 tgtacactgc ataatgcgga acagtgactt ctgacattgg acgaccatca ctaaaagttc
    23521 cttcaacagc atattgatgt gctaaatcta gttctacggt taccgtttgt ggatgggtaa
    23581 tttcatcagc aaaagtaatc gtaggagcaa ttgctccaac aattgcttga acagccaaac
    23641 tcaaaatagt taatacacat aatttctttt tttgtttttt atacatgaaa tgttctcctt
    23701 tcaaattaag cacaaaaaaa taagatgctc gcttgaacat ctttgtttat aaagtctttt
    23761 taaaattttt attgactgaa tagaatggat ttgagaatga atgagcctaa aaatttaa
//
LOCUS       DAJSMI010000025        23576 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJSMI010000025 DAJSMI010000000
VERSION     DAJSMI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.025"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ON430_000835"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ON430_000835"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR3218766.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ON430_000836"
     CDS             complement(1172..1867)
                     /locus_tag="ON430_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCR3218767.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ON430_000837"
     CDS             complement(1880..2596)
                     /locus_tag="ON430_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCR3218768.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="ON430_000838"
     CDS             2926..3531
                     /locus_tag="ON430_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCR3218769.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIDSTEE"
     gene            3856..4179
                     /locus_tag="ON430_000839"
     CDS             3856..4179
                     /locus_tag="ON430_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCR3218770.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="ON430_000840"
     CDS             4361..5731
                     /locus_tag="ON430_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCR3218771.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="ON430_000841"
     CDS             complement(5861..7162)
                     /locus_tag="ON430_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR3218772.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9350)
                     /locus_tag="ON430_000842"
     CDS             complement(7392..9350)
                     /locus_tag="ON430_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR3218773.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="ON430_000843"
     CDS             complement(9527..9793)
                     /locus_tag="ON430_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3218774.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="ON430_000844"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="ON430_000844"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR3218775.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="ON430_000845"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="ON430_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR3218776.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="ON430_000846"
     CDS             13137..13700
                     /locus_tag="ON430_000846"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR3218777.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="ON430_000847"
     CDS             complement(13953..14474)
                     /locus_tag="ON430_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCR3218778.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="ON430_000848"
     CDS             complement(14477..15319)
                     /locus_tag="ON430_000848"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCR3218779.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="ON430_000849"
     CDS             complement(15312..16037)
                     /locus_tag="ON430_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR3218780.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="ON430_000850"
     CDS             16213..16509
                     /locus_tag="ON430_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218781.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="ON430_000851"
     CDS             17077..18669
                     /locus_tag="ON430_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3218782.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="ON430_000852"
     CDS             18682..19422
                     /locus_tag="ON430_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3218783.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="ON430_000853"
     CDS             19590..20939
                     /locus_tag="ON430_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3218784.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="ON430_000854"
     CDS             complement(21067..21657)
                     /locus_tag="ON430_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR3218785.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="ON430_000855"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="ON430_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR3218786.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6754 a         4746 c         4489 g         7587 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcgattcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagtctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa aaaact
//
LOCUS       DAJSMI010000026        23520 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJSMI010000026 DAJSMI010000000
VERSION     DAJSMI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.026"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            265..2031
                     /locus_tag="ON430_000856"
     CDS             265..2031
                     /locus_tag="ON430_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCR3218787.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2133..3392
                     /locus_tag="ON430_000857"
     CDS             2133..3392
                     /locus_tag="ON430_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3218788.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3539..4840
                     /locus_tag="ON430_000858"
     CDS             3539..4840
                     /locus_tag="ON430_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3218789.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4843..5694
                     /locus_tag="ON430_000859"
     CDS             4843..5694
                     /locus_tag="ON430_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3218790.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5707..6522
                     /locus_tag="ON430_000860"
     CDS             5707..6522
                     /locus_tag="ON430_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCR3218791.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6673..7839
                     /locus_tag="ON430_000861"
     CDS             6673..7839
                     /locus_tag="ON430_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3218792.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8069..8716
                     /locus_tag="ON430_000862"
     CDS             8069..8716
                     /locus_tag="ON430_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR3218793.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8800..9744
                     /locus_tag="ON430_000863"
     CDS             8800..9744
                     /locus_tag="ON430_000863"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCR3218794.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9879..11330
                     /locus_tag="ON430_000864"
     CDS             9879..11330
                     /locus_tag="ON430_000864"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCR3218795.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11351..12187
                     /locus_tag="ON430_000865"
     CDS             11351..12187
                     /locus_tag="ON430_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR3218796.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12187..12846
                     /locus_tag="ON430_000866"
     CDS             12187..12846
                     /locus_tag="ON430_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3218797.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12972..13661
                     /locus_tag="ON430_000867"
     CDS             12972..13661
                     /locus_tag="ON430_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218798.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13667..14623
                     /locus_tag="ON430_000868"
     CDS             13667..14623
                     /locus_tag="ON430_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCR3218799.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14667..15179
                     /locus_tag="ON430_000869"
     CDS             14667..15179
                     /locus_tag="ON430_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3218800.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15435..15983)
                     /locus_tag="ON430_000870"
     CDS             complement(15435..15983)
                     /locus_tag="ON430_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3218801.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16072..17253)
                     /locus_tag="ON430_000871"
     CDS             complement(16072..17253)
                     /locus_tag="ON430_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR3218802.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17489..18997)
                     /locus_tag="ON430_000872"
     CDS             complement(17489..18997)
                     /locus_tag="ON430_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCR3218803.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19156..19347
                     /locus_tag="ON430_000873"
     CDS             19156..19347
                     /locus_tag="ON430_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218804.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19550..20467
                     /locus_tag="ON430_000874"
     CDS             19550..20467
                     /locus_tag="ON430_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCR3218805.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20460..21533
                     /locus_tag="ON430_000875"
     CDS             20460..21533
                     /locus_tag="ON430_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218806.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21681..22199
                     /locus_tag="ON430_000876"
     CDS             21681..22199
                     /locus_tag="ON430_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218807.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22180..22572
                     /locus_tag="ON430_000877"
     CDS             22180..22572
                     /locus_tag="ON430_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCR3218808.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22648..>23520
                     /locus_tag="ON430_000878"
     CDS             22648..>23520
                     /locus_tag="ON430_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCR3218809.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLP"
BASE COUNT         7734 a         4174 c         4711 g         6901 t
ORIGIN      
        1 atatacttct acgttaaaaa ctgttaaaaa acttttactt tagaaaattg gagaaattga
       61 tcgcttaaga taaattcagg acactaaata gtacaggaag tagattaaaa cttttaaaag
      121 ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa aaaactgtta
      181 ccgataacat ttttgaaagc gttgtctttt gtttctctat cacttatagt gatgacgtca
      241 tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg accggaaagt
      301 gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac taggaaaaat
      361 gatatcaaaa aagtatatgt gctttgtgga gatccatata ccataacaac tgaaaaatgg
      421 tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga ttattgggaa
      481 attgaagtga cgagcgaaac aaggcgctta cagtatgcgt tccatgttgt tggggaagat
      541 aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa tgtgattact
      601 gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt cactattcca
      661 gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc aaacggggac
      721 aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga ccgagaagac
      781 ttcttcgggg gagatttgca aggtgtgagg gatcatttgg attacttgac ggaccttggc
      841 gtcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa atacgatacg
      901 attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa aaacttgatt
      961 gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa tcatatgggt
     1021 gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat ctataaagac
     1081 tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt accagaaact
     1141 gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa actaaataca
     1201 gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat aaaagaattc
     1261 gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa
     1321 ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga gatttggcat
     1381 tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta tgcattcaca
     1441 gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga
     1501 atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt caacttatta
     1561 gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga attgatgaag
     1621 tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta cgggacagaa
     1681 atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg ggagaaggag
     1741 gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag gaaacagttg
     1801 tctaagatta tttctgaagg ttctactcag tggctaatag tatatgatcg tgaagataag
     1861 ctctatttca cacgggaact agagggacag atcatctatg tttactttaa tcagtcaaaa
     1921 gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg tcagttgtta
     1981 gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg aaaactgtta
     2041 ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa taagggtgta
     2101 acagcacgtt gaatacacag gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg
     2161 gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc aatggtaact
     2221 cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt gattctggat
     2281 ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca
     2341 agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat gggcctgctg
     2401 gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta ggacagcaag
     2461 gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca gacaaagccc
     2521 aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc cttgttttgt
     2581 actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg gagaatctat
     2641 ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga tttttagcta
     2701 aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc tatgtatttg
     2761 gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa
     2821 tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa
     2881 gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca atcattgacg
     2941 gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta gccaaaattc
     3001 ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga tgggtagtca
     3061 gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta acgaatcagg
     3121 aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg
     3181 aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa tatgaaacag
     3241 ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga
     3301 tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa
     3361 caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc atttttataa
     3421 aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat tatcttgctc
     3481 ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg gcattcagat
     3541 gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt cacttattcc
     3601 agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt tcgccatatt
     3661 cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag gattggttac
     3721 tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag gcactttgca
     3781 attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag acgcttatcg
     3841 agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg aagtcataca
     3901 taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc ttctcatgat
     3961 ttttacgatc attttcccag tcctagttac attgttcact gcatttacta attatgactt
     4021 caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact tttttagtat
     4081 cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg
     4141 gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca
     4201 ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac catgggcggt
     4261 cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg taggtgccat
     4321 caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca gtgcaatctc
     4381 ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg gctggttagg
     4441 atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag aagacttata
     4501 tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc
     4561 tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga atttcaataa
     4621 tttctccatg atctatctgt ttaacaatgg tggtcccgga agcgtaggcg gaggagctgg
     4681 ttctactgat attcttattt cctggatcta taaacttacg actggaactt ctccgcagta
     4741 ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg tgtcattgct
     4801 ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa atttcattcg
     4861 agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct tttaatactg
     4921 tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa gtcaggaaat
     4981 attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt tactcggcta
     5041 ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc tgtcctaaca
     5101 atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg ctatcgtttt
     5161 atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc gactatggca
     5221 gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa gtactggttc
     5281 ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat gaaaggatac
     5341 tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc agggcatttc
     5401 aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt tcaagcatta
     5461 tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct tcgaacacct
     5521 gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac aaaccaaaga
     5581 gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct attattcttc
     5641 tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg ataggagcag
     5701 agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc aactcaagtg
     5761 tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt atttttagtt
     5821 tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc gattccatta
     5881 gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa attttctgaa
     5941 cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt gacccctgaa
     6001 attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac ttttattgat
     6061 tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg ccgctatacg
     6121 atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga tttccaacga
     6181 tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc ttatagttta
     6241 agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc ttcctttttc
     6301 ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga gagtgcaaat
     6361 ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg gtttatccac
     6421 tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct gcttttatgg
     6481 gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta agcgatttat
     6541 gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt tctgaaaaaa
     6601 ttaaacttct tagttgattc ttttcttttt tagtattaac gtaatagggt agttttttta
     6661 aggaggaaaa tcatgttaga gaaaacttta tacaatcaat tattttcacg ttctttctcg
     6721 ctccctgtcg aagtaaatta ttgggacggt acaacgaaac aatacggaga tacggacact
     6781 cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat tacaagtaac
     6841 gcttctttgg cactaggaga agcctatatg gatcatcgaa tcgagatcga gggaagtatc
     6901 caagcattga tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg aaaagattat
     6961 ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga agatatccat
     7021 gcgcattatg acttaggtaa cgacttttat aaactttggc tagatccaac tttgacttat
     7081 tcttgtgctt atttcaatac acctgaagat acgttggaac aagcacaaat caataaagtc
     7141 catcatatct tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga tatcggttgt
     7201 ggctggggaa cattgatgtt cactgctacc aaagagtata atgtcaaggc aactgggatc
     7261 acactaagtg aagaacaata cgatttgatt accaaaagaa taaaagaaga acatttagaa
     7321 gataaatgcc gtgtattatt aatggattac cgcgaactaa aaggcgaaac gtttgatcat
     7381 atcacaagtg taggtatgtt cgaacatgta ggttctgaaa atcttgaagg ttatttcaaa
     7441 gtggtaaaag atttattgaa accaaaagga acagctttga tccatggaat cagccgacaa
     7501 caaggcggtg cgacaaatgc ttggatcaat aaatatattt tccctggcgg ttatgtccct
     7561 ggtgttactg aattagttga taatattaca aaaaacgatt tgcaactgat tgatttggaa
     7621 agcttacgtc gtgactacca attgacatta gaacattgga caaagaattt tcatcaagtc
     7681 caagatcaag tcacgaaaga aatgggtgat tccttctatc gaatgtggga tctatattta
     7741 caagcttgtg cagcttcatt tgaatcaagt aatatcgatg tgattcaata tttgcttgtt
     7801 caacctggaa acaatgacat cccaatgcat cgtgcataat tgaggcaaaa gtactgttta
     7861 aagactattt aaaaagaagc taagttttat gatgctccag aagtgaactt ttttgtctaa
     7921 cttctaggat tgtctctaaa gcttagtttc tttttcttta tattttcata ataaaagttt
     7981 ggttctttaa tttttaaaca tgacagaaaa ttttttcttc gttatacttg taatagaata
     8041 ctattttata acaaagggga tcgaatcaat gaaaaaaact gttatttata ctagtttatt
     8101 tgcagctttg tttttagctg cttgtcaatc aaataccaat caaggcacat ctgggaatac
     8161 gtcttccagt agcgaacagc gctcttcaca ggtatcggac tcctcaacaa aagcaagcac
     8221 atcttcgtct agccagacaa aaagttctag tgaagcaagt actcagtcag atatcaaagt
     8281 gtctgcagct cgtgcgatcg aattgttcca agataaatac ccagatactg caattactag
     8341 tttggaatta gatagtgatt ggggatctta cttctacaag atagaaggtg tcgatgatca
     8401 aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa atggagacag agaatccaga
     8461 aactctagat cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc tggatgttga
     8521 taaattgatc acgattgagg aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac
     8581 ggcaacagat tgggatttgg ataaagattt aggtactact tattgggaag tcaaagtaaa
     8641 agacggcaat caaagtacat cagtcaagat caatagccaa acaggcgaag tactgtcaac
     8701 tgaacaagat gattaagaat ttcttcggaa aacaagagtt gatttttctt ttagtcatga
     8761 tacactagac aaaataactt gtttgaagga gcgatttact tgagtttaga aacgaccttt
     8821 gccaatcata tctttgccaa tcctttgatg aatgcatctg gtgtccactg catgactacc
     8881 caggaattag atgaactggc tcattcagaa gcaggagcat ttatcactaa gagctgtacg
     8941 atcaatgaac gaaaaggaaa tcccgaacca cgctattttg atgttccttt agggagtatc
     9001 aattctatgg gacttccaaa cttaggtttt tcttattatc tagagtatgc tcttgcgtat
     9061 gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt ccattgccgg gatgagtgtc
     9121 caggaaaact tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa
     9181 ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac tagcttacga ttttgaggcc
     9241 acttatgaaa cactgaaaga agtattcagc atcttttcta agccgctagg aatcaaattg
     9301 cctccttatt ttgattttgc tcattttgat cagatggctg atatcttgaa tcagtttcca
     9361 ttgacctatg tcaatgctat caacagtgtg ggcaatggtt tgtatatcga tacagaacaa
     9421 gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca ttggcggaga gtatatcaag
     9481 ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc
     9541 attggtacag gtgggattcg gacaggacaa gatgcatttg aacatctgct atgtggcgca
     9601 agcatgttac agattggtac agagcttcat aaagaagggc cggaaatttt ttctagaatc
     9661 ataaaagaat taacgcagat tatgtcagaa aaaggatata cgtcaattga cgaatttaaa
     9721 ggcaaattac gcaccatttc atagcaaaga gacgaaggtc atgattgatc ttcgtctttt
     9781 tttataaaaa acactcgtct gttcattcct gtgcaagcgt actcattgta tgttagacga
     9841 acttgtggga aaataacaga ggcaaaaagg aggataatat gaatgatgca acagaattag
     9901 caaaagagct taaagaaaaa gaatttagtt ttcaagaact agttacggat atacagaaaa
     9961 aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta
    10021 attcccacga gctattgatt ccccagtcag aaaaagggcc attggctggt atcccatttc
    10081 ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc tacttcagga tctcgtttat
    10141 ttgaaacaca ccgagcttct catacatcta attatgtgcg gcaagcagaa gcgataggtc
    10201 tagttccttt tggacaaacc aatgcgccag agtttggttt caaaaatatt acagacccag
    10261 tcatctatgg cccagcaaga aacccttgga accttgaatg tacaccgggc ggatcaagtg
    10321 gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt agctggtgct agtgatggcg
    10381 gtggttctat tagaataccc gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag
    10441 ggacgatgcc agttggtcca tatgcttgga gaggatggca aggagctgct attgatttcg
    10501 gcttgacagt ttctatgaga gacacggagg ctttatttta tgggatgaga agtatccatt
    10561 ccggtgcgcc atatcaagta tcgccagttg agtggcaaac acatccaaga aaaaaacggt
    10621 tgaagatagc tttttgtgtt gattcaccga tccactcaca gattagtgaa gaggcagtac
    10681 ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg gcatgatttg acagagattc
    10741 cttacccact agatggtcgt aaactgatca cgtcttatta tcagatgaat ggcgcagaaa
    10801 cagcagcaat gatccgctcg attgaaacag gcatccagcg agctgtcaga aaagaagaac
    10861 ttgaacccat ttcttggact ttgttccaat atggtcagaa gctctctgcc gctacttaca
    10921 tcgattctct gcatgtatgg gatgaagcag caattgtcat ggaaaaccta catcaagaat
    10981 acgatctatt cctttcacca acgactgcat ttaccgcccc taaaatcagc gaagatttgc
    11041 aaagtgatgc gataagagca cagatggaac acatcagtga ttgttctgaa gtagaaggtt
    11101 tagacgttgt ttcttccatg tttgaggaga gcttgaagat cacaccttat acacagttgg
    11161 ctaatctgac tggacaacca gcaatcagct tgcctactca tttgacaaaa gaaaacatgc
    11221 ctctaggaat ccaatttatg gctgccagag gaagagaaga tttgctattt caaatcggct
    11281 atctatttga agcaaatcat cagttccatt taccaaatcc atggaaatag cagaaacaag
    11341 gagaagaaga atgaagaaaa aaagattgtt tgctctaata actagtgcct tttttatttt
    11401 aagcgcttgc ggtacagctg ccgctaatca ggaagataac agctggagta atgtagaaaa
    11461 aaagaaagaa ttagtagtag cgacttctgg cacactgttt cccgcatctt attataatga
    11521 tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa gaggtggcta aacggttaaa
    11581 tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa atcacttctg tttcaaaaag
    11641 agaatcagat tttgctgcga acgattttgg tatcacaaaa gaaagagaaa aaaaattctc
    11701 cctttcagag actttcaaat attcatttga cagtatgatc gtacgaaaaa aagatgattc
    11761 aggaatccac tcacttgaag atcttaaagg gaaaaaagca gctggcgaac ccaatactag
    11821 ctatatgcgt ttagcggaaa aacacggagc taaattagtc acatatgaca atgccacgaa
    11881 tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat gttattctaa atgattatta
    11941 tctgcagaaa atggccgtat ctgctttacc agatatccct gtaaaaatac tggagaacgt
    12001 ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa
    12061 gaaaatcgat ggcgtattaa aagagatgaa agaagatggg acgacaaaga aaatatctga
    12121 aaaatatttt caaactgatg tttcagtcaa accgaaagaa acgatggctg agtcggtgag
    12181 tgagtgatgt actttcctct aattgatttt tccctcatgt ggacttcact tcctttcgta
    12241 ctgcaaggct tggtatacac attagggatt ggcttcgtgt cgtttctttt aggaaatttg
    12301 gtagggttgc ttttgacagt tttaggtctt ttggattggc tgcctctgaa tgtatttatt
    12361 cgtttttatt tatcgttctt tagaggcatt cccgcactag tcctgctgtt tctgttatat
    12421 ttcgggttac cttatcaatt aagtgcctta acggcttcag tcatctgctt tacgattacc
    12481 agcagtgcat ttattggtga aatctatcga ggatcactag caggcgtttc ttcaggtcag
    12541 tgggacgctg gtttagcttc tggtttttct ttctttgggg tattgaaata tatcgttttg
    12601 ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg tagctatgga tctagtgaaa
    12661 ggtacttcgt tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc aaagatcata
    12721 ggcggtcgga cattcgatta tttttctctc tatattttag ttgcactgat ttattgggtc
    12781 atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa aacgttctat ccatcagtca
    12841 atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat ttctattttt tgtttcttgt
    12901 atattctgcc tagtatttta aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg
    12961 aggtagaaaa catgaagacc ccgctaacca tcgaaataga atcctcgtta taccaatatt
    13021 gcatcgagca aggcggcttg gttgtagaag aagtcacgat gcctgatgac caaggaattg
    13081 tagatacgct tagctgcttg acaaaatttg atggaagccg agaatggcga tgctatgagc
    13141 tgaaagtgac aaaagcagat tttcgctcta cagccaaact gtcatttgtt ggacattata
    13201 attattttgt tttaccaagt aaactatatg aagaagtaaa aactgaaatc cctacaggaa
    13261 taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc tctggtacat
    13321 ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt accggaagaa gctttgacaa
    13381 accgcttcat ggcttcttta ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg
    13441 taaaatattt tccaaaagat caattgtaca aagaattgaa aagacgttca gaaaatcaag
    13501 atccattttc taacgaacga tattatgaac ggtttttaga ggaaatagct agtcaggcaa
    13561 tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac
    13621 aacagcagat ccgtcggttg cctacagagc cattggagtg aaaaatatgt actatcctta
    13681 tttaagagga aaacaatttg acctattagc attaaaagaa gcactgtcgc gaggattgct
    13741 ctccaataaa atccaaccag tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt
    13801 gatcgaatta tttcaaaaaa agcaccatcc aatcgctgtg atccaaaatc cccaagtagg
    13861 acaattcaaa ttattcgatc agcatgtgca cagttggaca gtcaaagaaa atagctctgt
    13921 cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa gtactggatt cacctccagc
    13981 gtttcttgtt ttcgatggac agcactaccc aaaagataca gaggtgtgga aacagttggc
    14041 aggagttgat tcgaaatttt tgataccaga tactagtcgc tttcgcatct ggctgcccga
    14101 aaacaaaatt gtgatccgtg actcttttca aacgagaaag catgttgaaa gttatgcaga
    14161 taaaacagat gactttttta gtgatgacta tctttttttc cacgcagacg gatatattgg
    14221 attttctgat ttcacgatcg aaggtagccg ttatttcgat aaagggttcc ctagccgagc
    14281 gcttgctatc catctaacgt atattgatgc ttacggcaat atacgcgtga aacattttgt
    14341 ctctgacagt aacgactctg ctaaagacca agcattgaag tttcttgaag caggagataa
    14401 aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg attacttcag gaatgattga
    14461 attattggct ctttaccagc aacaaaaatt ccctggactg ggagtgttga aaaagtggtc
    14521 acttatgcat catttggaac ttattagtca cctgctagat catccaaaag attggcttaa
    14581 atattattca aaagttcagg aactatatga agtgatccaa tgattagata atgtaaatga
    14641 aaaaggaata gaaaagagga aactggatgt atattcggca aacaaggaaa gaagaagtag
    14701 aagaagtggt agaaatcatt aatgccgcag cggatttcat tgcttcgcaa ggctctcctc
    14761 aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag
    14821 aaagctatgt attaatagtg gaagatcaaa ttgctggaac agctgcatta gtctcaggcg
    14881 tggatcccgt ttacacaaaa atagatggta gttggaaaga atcaactggt ccttatttat
    14941 cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca aaaccgttct
    15001 tagaacattt gttgacgatt gcagccagca aaaatatcca agatatccgt atcgatactt
    15061 atccagctaa tgaaccgatg gaaaagacca tctattctgc tggattcact tatcaaggga
    15121 tgattgaatt tccttttgaa cacggcgaaa gaaaagcata tcaaataatc atcaattgac
    15181 tataaaatca tctgagcaaa gataaagaca ttaacactac tgaaaaaaac aatcatatga
    15241 tagccaagag gttgtaacag aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa
    15301 tgcttctcaa atttttgtgg aattttagcg tattttacga aatagctgct ttctgaaact
    15361 cccttcatcg agaggtcgtg acatagcttt tgtcacgatc tctcggctta tcctacgagt
    15421 attattcgat acatttatat caataatcct ttttttattt tatctgcagc ttcttggaac
    15481 gctccatcgt ttgacacgac taaaagtgag gggatagctt tcaaacgttt caactgtgct
    15541 aaatcttcta cataaaccgc cagtctggct gaaatctgtt cagatgaatc tccacgtttt
    15601 ttcaggcgcg attgcaatgt ttctttagag atcgtaataa agataggacg gactaccgtc
    15661 ccgaaatact catataaatg ccaaaaacca tttgctgtca aaacggcgta acaatttcct
    15721 actgtcagtt tctttttaat ttctgcttta ctggaaccat atttatgttg agcatattgg
    15781 tcccactctg caaactcatt tcccttgatc atctgattga atgtattgtc agaaacaaaa
    15841 taataatcct cttgatcttt ttccccaggt cttggtgatc gattcgtgta agtaatgatc
    15901 ttttgttccg gctgaaaaat agcttctgcg agtgaagttt ttccagaacc agaaggaccg
    15961 ataaatacat agcaataatt cattgtcatt tctcctttga tgattgaaca agtgaaaaag
    16021 ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc ttttttgtat tttataccag
    16081 caagccagtc acaatagcac ttaaaaagct gactaaagtc gctgttagca aaattttcaa
    16141 tccgtgtttt gcgacaagat ttcctttttc ttcatttaat cccttcatcg caccagaaat
    16201 gatcccgata gaagagaagt tggcaaatga aacgaggaag acagatataa ttgctgttgt
    16261 acgctcagta aaatggaaat taccatttgc taattctgtc atagcaacaa attcatttgt
    16321 aaccagttta gtcgccatga tactaccggc atccactgct tctttccatg ggattccgct
    16381 gatgaaggca agaggggcaa agatgtaacc taggatctct tggaaagtga tgccgaatat
    16441 cccgtggaag atagcattga tcatggcaat caaagcaaca aaaccgataa gcatagcagc
    16501 aaccgtgatc gccacatgga aaccatcaag aatgtattct cccaacattt ggaaaaatgt
    16561 ctgttctttg ttttcttcaa tgatcagctc atcttctttt tcttctaata catagggatt
    16621 tacaattgaa gccagaatat aaccgccaaa taagttcaaa acaagggcag tgatcacata
    16681 tttagaatcg atcagtgcca tataagatcc gacgatcgac atagaaactg tagacatagc
    16741 cgatacactc atggtgaaca aacgtttctc agataggaag ggtaattcct tttttatgga
    16801 aataaaaact tccgattgtc ctaaaatagc agaagctact ccattataag attccaattt
    16861 gcccatcccg ttgattttac ttaatcctag accaacagct ttcataaata atggaagaat
    16921 cttgatatag cgtagaatac cgataatagc agaaataaat acaattggca tcaatacgga
    16981 aagaaagaaa ggtgcacttc ctttatttgc tacaccggca aaaacaaagt caacaccttg
    17041 cccagcaaaa gcaagaagat gatcaaacat ttttgccaat acagaaacaa ccgctgtacc
    17101 aaaagttgta cgtaataata cgaaaccaag aacaagctgt aaaacgaaca tcacaattag
    17161 tggtttcaat ttgattgctt ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac
    17221 aaataaacca ataataccta ataaataact caaataatca ctcctattcc aaattccgtt
    17281 ttattttacc tgttttgcaa ataaaatact aatctttagt gaaattttca ttactttttt
    17341 agagtgaaac gcattcattt tccattagaa aataaagcga acaaaataaa aggtttaaaa
    17401 agtgtaacgt cttagtaaag tctataaaaa aacgaaacta tcgattaagc taatagtttc
    17461 gttttcaaca tttataagta aactttttct attggtaata tagattttcg gaagggtaga
    17521 caggtgcgca ggtaatatcg attcctagtt ttttcaatgt ttgttcatca tttttattga
    17581 ccatcacagt agagtgggct tgtgctccct caagttcagc tagtttatca taagcaactt
    17641 gagcgattgg attcgttact gcactgattg ataaagcgat caagacttca ttgatgctta
    17701 gtgcatccaa agaggaatga agtgtctttg atttcaatcc ttgtattgtt tcaagaacta
    17761 atggtgacaa taaaggaatg tcatctgcga tatgggctaa atgttttact gcatttaaga
    17821 taactgccgc tgacgcatcc atcaatgaac ttgtacgccc ggtaatgatg acttcatctg
    17881 gtaattcgat tgcaatcaca gcagaaggat cttttgtagg actttgcgct tgaatcttat
    17941 ctgcatagtc atgggcaggg actaccacgt ttcggtcttc aggacgtaaa ccgacttctt
    18001 ccataatcag tttgattcga ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc
    18061 attctgtttg gaaataacga cggatgattt cttgtttaga ggcttcttgg acaacggtat
    18121 catccacgat cccaaatcct acacgattca cacccatgtc agtaggagat tgataaacag
    18181 attctttacc agtaattttt tcaataatac gtttgattac aggaaatgtc tcaacatccc
    18241 gattatagtt cacagctact ttattgtagg catcaaagtg gaaggaatcg atcatattga
    18301 catcttttag atcgactgtg gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa
    18361 cattccatac tgggaaagtt tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt
    18421 catgatacag ttggttcaag caagtagcga gctttccact tcctgggcca ggagctgtca
    18481 ctacaacgat tggtttagtt gtttcaatat actcattttt tccaaaacca tcttctgaga
    18541 cgattttttc tacgttgact ggatagtctt cgatttctga gtgtttatag accttgatgt
    18601 ttcgtctttc tagtttattg ataaagactt tcgtggcagg ctgtccacta tagcgagtga
    18661 taacgacact gttgattaat aagccataac cacgtaattc atcgattaac cgcaagatat
    18721 ccatatcata agtaatccca taatcaccac ggatcttatt acgttcaata tctccagcat
    18781 agacgcaaat cagtatttca gcttgatctt tcaatttttg cagcaatttg attttcgcat
    18841 cttcttcaaa tcctggaagt acacgtttcg catgcttgtc gtcaactaac ttcccaccaa
    18901 attctaaata taatttgtca tagtggttca ctcgttccaa aatatacgct gactgctctt
    18961 caatatattt ttctgaatca aaaccaattt ttttcactag gttccctcct taagtactaa
    19021 agcatttttt catttttttg aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac
    19081 aaagaaaatg gtttaaacca acttgtctag atagttattc attggtatga tagtttttga
    19141 taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa acgcatataa acactatatc
    19201 cgttcgattt ttaccggtta tgaaagaatg cttgaagatc ccgaattgga acaattgacg
    19261 tttacttatc taaacgaaga aacgcagcta actcgtgatg agcatcagcg cattcatttc
    19321 acaacaagag atctgcctag taaataaaaa ataagataaa ggtcatcgga ttcgattctg
    19381 tcctatcaga tggaaagact agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc
    19441 ctgttttatg gagcattgcc cgctcttttt tataacaatt ttttacagaa aatggtacta
    19501 ttgaagtatt aataagtgaa aaacagatga gtaagaggag gcaagtagta tgaaagagcc
    19561 agatacaaat gcaaattatg agccagacga actagaggag acgaacgcgg atcaggatac
    19621 aggacaagaa tcgtcttctt tttacgatat caaagaaaaa gcaagcatga atctttccca
    19681 attattagaa gacttacagg cttttgaaaa agctgtccgc gaagaagata tccctgagat
    19741 ctaccgttta tacaatggcc gattgaatga agaattaaaa agaagctcaa atgaaaatca
    19801 cgagatcgat caattgctga tgcgtaagat ccatgatcgt tttttacagg aatttccttt
    19861 tatggaacaa gtagaaaaca tctcgccaac gatgagctat tacaaaatcg gcacttatta
    19921 ccatgatcgg cccacggtcg gtattgatgc aagtctgcca gaaatttttg ttttgcctaa
    19981 gatcgatgaa gagtgggaga aatattccca accggagtca acggactatg acaaaaaaat
    20041 caatgagcta gacgcaaagg tgatcactgc tcagacagaa attgaacgtt tagatgaaca
    20101 aataaaagaa atcaatcgac aaatgactga tttgaacgac aataaaggat tcttgaatcg
    20161 taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag aagcaagtac tatctaatga
    20221 aaaattaggc tggcttcctt atattgagac gcctgaaaca atccagcagc aaaaagaagc
    20281 attgaagcaa gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag
    20341 gcaaatcaga cgctatttcg gtagcaaaga gggattcggt caagcaatcc atgaattctt
    20401 gatgaattat cttggcaatg agaatcctaa taatcaatct aatgaaggag gttccgagta
    20461 tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga acaaacagaa gatcatgaag
    20521 aaacgaacac ttcattggaa gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc
    20581 ctatgctaga ggaaacaaaa atacatgaaa aagaaaaagt aactccgacg aatacgaata
    20641 ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag
    20701 agcaagatca tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt
    20761 tacgtatgag aaaagatttg atcgaatcag attttccaca attattagaa tcatatgatc
    20821 gttttatatt gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg atcgaatact
    20881 tcacgtatga attactgaaa ccgttgaatg atattggtct ggaacggtca aattcagctt
    20941 atgacacatg gaaaacaaga catttttcga tcaattatac gttgaatgga caaaacgaat
    21001 tagtcttttc ctttgtatta ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc
    21061 tcctaaacgt atcaccagag acaatggaaa tcaatgtcca agatgaccgg gtgctttctt
    21121 tgatccgaca ctggtcagtg gatcgagtat tctctgcagg gcagatcacg atattcaatc
    21181 acaaattgaa tcaattatta gcccatgcaa gaacacttgg ttttaccgtt aatcagacat
    21241 tactagacaa tacgaagcca cttcgtttga atcttcagag cgaatttgaa ttgacgaatg
    21301 aagtattgga tgatattttt atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga
    21361 tcaacgaaac tacttatcaa gtacgattag ataaaggaca gtctttaacg ataacaacaa
    21421 acgaaaaaga tcaaacagtc ttgtcgatct catctggaga ctacccacga tctgtcattg
    21481 atttctttat caattacgaa ttcttggtcc cattaatggt gagaaagtcc tgattggata
    21541 agttggatta ccagatgtca ttcagataat tctgttccaa aggacttgcg gcagttatcc
    21601 agaataattg tcacaagtct tttctgtatg tactaaatgg acgtgaataa cttgctgatg
    21661 aaggagggat ttcattagga atgtccacaa ataaaatagc acagcttagc ttattgtctg
    21721 ctgcttgtgt agcaggtagg atcgcttttc aatttattcc gaatttccag ccaatgactg
    21781 ctattttttt atttatagta ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc
    21841 tttctattgc gatcagcagt ttttattttg gtgttggacc atgggtcatc ggccaatgga
    21901 tcagctatac tgttgttctg agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa
    21961 ggaatctatg gttaaaggga gtctgttttt ttctggcggg catggtttac ggaataggta
    22021 tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc ttggatctat taccttcaag
    22081 gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt gttctttctt gtttttctgc
    22141 cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt
    22201 ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt gcagtacaac ctatacacaa
    22261 tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa
    22321 gaaaatggtc aaaacttttc ggaaaaagaa gtggtcttca aaaaaggcga tgatttactg
    22381 agtattttga aacgaaattt tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa
    22441 gggcactcac aggatgagaa aaatcaactc tattggatgt ttactgtgga cgggaaaagt
    22501 gctactactg gtgccaaaga gatcaaattg aatgatggac aatcggtcat tttcaatttg
    22561 tctaaactat aagtttcata aaaaactgtt aaagggcgaa tattgccata attcagtgag
    22621 ttcagttgat gaaagaagga aaataacatg gaaaatttta aaaaaagcgg aatgttttcc
    22681 gtaattgctg ctctaggggc taatattcta gttgctatca gcaaatttgt cggctatgca
    22741 atcagtggca gtgccgccat gctaaacgaa agcatccaca gtatcgttga ttgcagcaat
    22801 cagattttgc tgttattcgg tgataaacaa gccaataaag gacagagcga gttgcatcaa
    22861 tttggtgaag gacgagcaaa atactttttc agtacaatcg tcgcaatgat gcttttcttc
    22921 ggcggtgggg cacttggggt gatggaagct tttgaaaagt tgacgcatcc atcccatgaa
    22981 gtgggtaatc cgatcattgt tatagcaata ctgttatttg gtttggtaat cgaaggaagt
    23041 tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata ctgaaaaact ttccacgatg
    23101 cgctttttgc gcgaaagtcg acacagtgaa atactgatta tttttacgga agacttgtgt
    23161 gctgtaatcg gtttggtctt ggctttagca ggaacaatat tgacttatgc aaccgggatt
    23221 gctgcatttg atgcgatcag cggattgctg atcggtttct tgctgatggg agccgccatt
    23281 ttcctagcaa aagaatttta tagtctgatc atcggtgaaa gcgtgacagc aagcgattta
    23341 tcaaaaatca agttagcttt tgaacgtcct gagatcagtc ggctgatcga tataaaaaca
    23401 gttcatttaa gtccaacgga gattttagtt gcagcaaaag cagatatcgt tggtaacaaa
    23461 gagaatgaag cttactcggt catcaatgat attgaaaaaa atatgcgtca gtctcttcct
//
LOCUS       DAJSMI010000027        23104 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJSMI010000027 DAJSMI010000000
VERSION     DAJSMI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.027"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..201
                     /locus_tag="ON430_000879"
     CDS             <1..201
                     /locus_tag="ON430_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218810.1"
                     /translation="VLINKNDKNQGFLQFYGAAYAVLGVMGILTAFFNQRFIALVFLL
                     IVIIVSATFSIRFAKKIAEPKQ"
     gene            274..753
                     /locus_tag="ON430_000880"
     CDS             274..753
                     /locus_tag="ON430_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3218811.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            976..1119
                     /locus_tag="ON430_000881"
     CDS             976..1119
                     /locus_tag="ON430_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR3218812.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            1697..3280
                     /locus_tag="ON430_000882"
     CDS             1697..3280
                     /locus_tag="ON430_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3218813.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(3717..4901)
                     /locus_tag="ON430_000883"
     CDS             complement(3717..4901)
                     /locus_tag="ON430_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR3218814.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(4926..5933)
                     /locus_tag="ON430_000884"
     CDS             complement(4926..5933)
                     /locus_tag="ON430_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3218815.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(6105..6464)
                     /locus_tag="ON430_000885"
     CDS             complement(6105..6464)
                     /locus_tag="ON430_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218816.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(6436..6807)
                     /locus_tag="ON430_000886"
     CDS             complement(6436..6807)
                     /locus_tag="ON430_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCR3218817.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(6824..7126)
                     /locus_tag="ON430_000887"
     CDS             complement(6824..7126)
                     /locus_tag="ON430_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218818.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(7143..7613)
                     /locus_tag="ON430_000888"
     CDS             complement(7143..7613)
                     /locus_tag="ON430_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCR3218819.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(7588..7866)
                     /locus_tag="ON430_000889"
     CDS             complement(7588..7866)
                     /locus_tag="ON430_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR3218820.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(7863..8912)
                     /locus_tag="ON430_000890"
     CDS             complement(7863..8912)
                     /locus_tag="ON430_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCR3218821.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(8869..9798)
                     /gene="tadA"
                     /locus_tag="ON430_000891"
     CDS             complement(8869..9798)
                     /gene="tadA"
                     /locus_tag="ON430_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR3218822.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            9930..11684
                     /gene="ade"
                     /locus_tag="ON430_000892"
     CDS             9930..11684
                     /gene="ade"
                     /locus_tag="ON430_000892"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR3218823.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(11782..12444)
                     /gene="trmB"
                     /locus_tag="ON430_000893"
     CDS             complement(11782..12444)
                     /gene="trmB"
                     /locus_tag="ON430_000893"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR3218824.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12514..13296)
                     /locus_tag="ON430_000894"
     CDS             complement(12514..13296)
                     /locus_tag="ON430_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCR3218825.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(13500..14711)
                     /locus_tag="ON430_000895"
     CDS             complement(13500..14711)
                     /locus_tag="ON430_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218826.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(14704..15441)
                     /locus_tag="ON430_000896"
     CDS             complement(14704..15441)
                     /locus_tag="ON430_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218827.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            15579..16013
                     /locus_tag="ON430_000897"
     CDS             15579..16013
                     /locus_tag="ON430_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCR3218828.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            16006..16350
                     /locus_tag="ON430_000898"
     CDS             16006..16350
                     /locus_tag="ON430_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3218829.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            16530..17540
                     /locus_tag="ON430_000899"
     CDS             16530..17540
                     /locus_tag="ON430_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3218830.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(17609..18553)
                     /locus_tag="ON430_000900"
     CDS             complement(17609..18553)
                     /locus_tag="ON430_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3218831.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(18537..21257)
                     /locus_tag="ON430_000901"
     CDS             complement(18537..21257)
                     /locus_tag="ON430_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3218832.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(21254..22474)
                     /locus_tag="ON430_000902"
     CDS             complement(21254..22474)
                     /locus_tag="ON430_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCR3218833.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(22755..>23104)
                     /locus_tag="ON430_000903"
     CDS             complement(22755..>23104)
                     /locus_tag="ON430_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HCR3218834.1"
                     /translation="FIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGV
                     VFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDK
                     DGVSQAFERILTI"
BASE COUNT         7114 a         4669 c         3996 g         7325 t
ORIGIN      
        1 gtattgatca acaaaaatga taaaaatcaa ggattcctac aattttacgg agctgcatat
       61 gctgttttag gcgtaatggg tattttaaca gcttttttca atcaacgctt tattgccttg
      121 gtctttttac tgatcgtaat tattgtttct gccaccttca gtatccgatt tgcaaaaaaa
      181 attgctgaac caaagcagta aataattctc tttttcccta aagtgttcta caataaaaga
      241 gtacaatacg attgacttga ggagtgaatg cttatgttac aacaatacaa aaaaatcatg
      301 gttgccgtag atggttctga tgaagctgaa ttagctttta aaaaggcggt caatgtagct
      361 atccgaaata acggagaact tctattagca catgtcattg acacacgttc tttccaaacc
      421 gtttcatcct ttgatggaat gcttgcagaa caagctactg aaatggcaaa gcaaacattg
      481 gcagattatg aaagcaatgc gaagaaagct ggcttgaata atgtaacctc tgtcgtagaa
      541 tatggctcac caaaacaaat tattgccaga gaaatcccag aagataatca agtagatttg
      601 attatgttag gagccacagg attgaatgct gtagaacgtt tatttattgg ttctgtttct
      661 gaatatgtta ttagaaacgc agcttgcgat gtgcttgttg tccgaactga tttagaaaac
      721 caattgccag ctaaagacca caccaaagct taacacgtag acgacctgcc aactatcggc
      781 aggttttttt gcacaaaaaa ccgtgataaa aacaaaagac gcggcaagca ctcatcggtc
      841 tttataatgt ttaaaataaa gggaaaacag tcaatgagtt caagtaaaat aaaaaaaagt
      901 gaacaccagg gcgattagtc gccctggtgt ggtttaaaac gacctaagca tgccaaagct
      961 tattggttcg tcaatgtgga agtttccgat gaatctgata cgaaaaaata tgaagtatcg
     1021 atggcggata ccgattttat cgccaaacta aaattttatt tcgggttcgg gtaccgcgag
     1081 aaggcaagtg ctacattcgg taaatcattc aagaattgat gaaaggctac cgcttgccat
     1141 tgcaaccaca acgatactcc tgaacgcgat gttggtgaga tttccaaaga gaagagctgt
     1201 ttaatgcaac ggtattaagc aaatggcaaa aggaagtcat gtagacaaag ttttttatca
     1261 aacaccatat gatttcgcct gaacgctggt ttagctcaga atacaatgat gtggtccacg
     1321 agacattacc ctcagtcaga taaaaaaagc tctctgacag aattacacat cgataaatat
     1381 taagtaaatg tcagcgctag ttgaactttc tgacactttc aggtacactg gaaaagacaa
     1441 ctacacgaag gagggaaaat actatgatgc gcatgactaa aataacaagt aatgaaaatt
     1501 accaacgata ccatatcgca tttagccatg caattggttt tacagttgct gttgacggga
     1561 caagtttgtc caaaacagtt gaattcacgt aaaatcaatg gccatctcaa ggtatttttc
     1621 tcatgataat ctgagtttaa ataacacagg ccatggataa ccatggcctt tttgagtggt
     1681 ctcaaggaga gagatcatga tcatacaagc aatttctatt aaaaaaacaa tcggcgggaa
     1741 tccattattt gaggatttat cctgtcaaat tgaagctggt gaaaaaattg ccttagtcgg
     1801 tatcaacgga actggcaaat caaccttttt acaaatcttg accggcagag aaggtgtcga
     1861 cagcggtgtt atcagtcgca aaaaaggctt aaagatcggg acggtcgaac aagaacttac
     1921 agtttccgaa gcgaccgtca accactactt gctccattct gccgctgaga tacaggacct
     1981 caaacagcaa atgagtcgct acgaagcatt gatgacagaa ccagatacaa acttagagaa
     2041 gtgtctggcc cgttacggtg agctgcagca tcgctttgaa gaactaggcg gttacgtatt
     2101 agaagaccgg attgcagcga tcttgcaagg cttagggatt ccacaccgtc aggaagcacg
     2161 gttaacagaa cttagcggag gcgaacgagt taaagtcgct ttagccaaaa ttttagcagc
     2221 cgatgccgat ttacttttac tcgatgaacc gaccaatcat ttagatctta acagtattcg
     2281 ctggttggaa aactatttac agaatacaaa aaaatcggtt ctggtcgttt ctcatgatcg
     2341 gcaatttctc gatcaagtca ccacaaaaac atgggaactg gaagaaggtg cattgattga
     2401 gtatcccgga aactacagcc gttttcgagt cttgaaagaa gctcgcttgg ctgagctgac
     2461 aaaaaattac gaactgcagc aaaaagaagt ccagcggttg aaagtcatga ttcgtcgttt
     2521 tcgtcagtgg gcacatgaag gagacaatga gtccttcttc aaaaaagcca aagaactgga
     2581 acgccgcctg gcgaaactga cactcgtcaa accgccacct cccccgaaaa atcggctgca
     2641 aagcttgtct aatggtggaa aatctggcaa agaagttttc atcattcaaa acttgcacca
     2701 gcagtacgct gatcaagtac tattcaaaga cagcagtttt gcagtctatc gtggagatca
     2761 tttagccatc atcggcgaca atggtgcagg aaaatcaacc ttactcaaat tattactcgg
     2821 tgaggaaaag ccttacggtg gcaccatcaa attaggtagt agcttgcaaa tcggttattt
     2881 gccgcagcag ctgcagtttg ccgatcccga tagccgtttg ctggcttacg ccatcacatt
     2941 gactggaaac gaagaagtcg ctcgacggca attagccaaa agtggctttt atcaaagcga
     3001 tgtgggtaaa cggatcaaag atttatccgg tggtgaaaaa atccgtttgg cgttgctgaa
     3061 actcttttta gaaaaaatca acgtcctgat tttggatgaa ccaaccaatc acctcgacag
     3121 ctatgctcga gaagaaattg aagaaatgct gcaagactat caaggaacgc tcctagcagt
     3181 cagccatgac cgctactttt tgcagcaaca cttccaagaa gcactcgtta ttgatcagga
     3241 gcagatcagc cgccagccgc tcggccaact gattgactag gacgtttttt cgcaacaaaa
     3301 aacttgattt tcatacctgt gctttcacga ttcaggcaaa gtacaggtgg aaaatcaagt
     3361 ttttattatt cgatcaatga atgtttcgga tcggttttct agctaaacat aaagcgaaga
     3421 ataaagtcga cagcccacga ggctgatcta aaaatgtttt ttctttttac atatgtaatg
     3481 tcccttataa agatgaatga aaataaaata ttgggatttt cctgaaaatc aataaataaa
     3541 atgccagtct tttccctttt gaccactttt ccaagcaaaa ttccctaaga agatcctgta
     3601 aaaaagaagg gaaattgaag ttaaattacc attttgcggt cttctccgca aaaaaaacac
     3661 atgcaagaat tccctcttgc atgcgtttca tcttttatcc taacgaatgc tttggcttag
     3721 ccgcgcagac gctccacatc acgggcaatc attagttcct catctgttgg gatcactaat
     3781 actcttactt ttgattcttt tgtagagata tctacttctt caccacgcaa ttcattcaac
     3841 gctggatcga tttcacaacc aaaccaagac atacctttga tgatttcact acgtacatgt
     3901 ccgtcattct cgccgatacc agcagtaaag acaattgcat caacaccatt catcgttgtc
     3961 acataaccgc cgatgtattt tcggatgcgg tccacgaaaa tgtcataagc taaacgaata
     4021 tgttcttttt catagttctt ttcaagatca cgcatatcac tagacaatcc tgttaggcct
     4081 agcagaccag atttcttgtt caaaacattg atcatttcat caatatcaat ctctaatttt
     4141 tccattaaat atgggagaac agctggatcg atatcacctg aacgtgtacc cattgtcacc
     4201 ccagctaatg gtgtgaatcc catagaagta tccatggatt tacctccatc gactgctgtg
     4261 atcgatacac catttcctaa gtggcaagtg atgattttta gttcttcgat cggtttgttc
     4321 aataattttg ccgcacgttc cgacacataa cgatgacttg tgccatggaa gccatatttt
     4381 cttactttga aatcttcata ataagacacc ggtaagctat ataaataatt ttgctttggc
     4441 attgttgagt ggaaagatgt atcgaataca gccacactca aaatgtcagg taaaatcttt
     4501 ttaaacgctt tgatccccat taagtttgca ggattatgca aaggagcaaa ttctgctaat
     4561 tcagcaatgc ggtccattac tttctcatcg atcactactg aatccccata agtttctcct
     4621 ccgtgaacaa cacggtgccc tactccggtg atttcatcat aagagcttaa aatttgcagt
     4681 tcaatcaact gatctaacag catttttact gctacttcgt ggttttcgat atccatgatt
     4741 tgttcgaatt tttgattatc gccgtacttg atggtaaaaa tagaatcttg taaaccgata
     4801 cgttcgacga tccctttcgc aactacggtt tcttctggca tttgatataa ttgccatttc
     4861 aaacttgaac tcccagcatt gattgcaatt gttttagaca tatgactctc cttctcgttg
     4921 aatgattaca gagcagatga tttccagttt ttaaactcgt tgaaaaattc cgtcaccttc
     4981 tctggttctt ttaatgaagc aagtttcaca agcaagactt cttttacttg ttgtgcttgt
     5041 ggtccttttt tctgtaagat caaaatactt ttttgcgatt gtttattacg gaacaattca
     5101 tctggcaatt gaatcattcc ttgaagataa ccttcttctt taaaccattt tttgatttct
     5161 tcactttgat ccgtttctaa aaatccactc ggcatcaaaa acaaaccaaa accatccggc
     5221 ttcacatatt tcattgattg ctctagcaat aaatgatgtg cataactatg gccttcttcg
     5281 gtacttgtta aaaactcttt tgctttttcg tcattcggat aataaccgat tggcaaatcg
     5341 ctgattgcaa aatcgacagg atctattaat aattcctgca acccgtcttg atgaaagtat
     5401 tgaacatttg cttgtgtcaa atcacttgta gaagctgcga cagccaacaa tgtatcatca
     5461 atatccacac caattccttc tgtttggtac cccgcattcg ataggtttaa aagaactgtt
     5521 aataacaagt tgcccattcc tgccgcaata tccagaatct taaccggtgc ttttttattt
     5581 gtgtatagtt gttcaataag aaaaacaaat aaaaaaccga tactatcagg agtgagttga
     5641 tggttcgcct gcaaatgttc tgtctggctg cccttcaata aaagtaattg agacagtctg
     5701 cgccattctt ctggctcgaa agaaagtttc ttcaactctt cataaagagc tgtgatccgt
     5761 tgcgttgtct cttttgtcgg cactccatcc accacacgta cttgataatc atctattaaa
     5821 ttttctgcat tttcgacata ggcttctaaa aaggaagttc ccaatgcatt ctgtaaaagc
     5881 tgaatagctt ccaggttttg attaaaccct tgttccattt tttctggtaa catggtgcac
     5941 ctcatttcta gatatattat aacaattttt tcacctgttt aattctatcg aacaattgac
     6001 aaaatttcaa tcgttttcac tgttttttat ttgtgaaacg gcaaacattt ttttattaaa
     6061 aacagtgtta taacagaaaa gagggcgttt tcacgaacat tccatcattc tgtactagta
     6121 tctgtatcat tttttacttc ttctgtgaac cgaaagataa acggtgagac ttccacctct
     6181 aagaataggg actgttgtac ccactgatat gttacagatc cagttgtgta gtaaaaaact
     6241 ccttccgaac tttcggcaag cgtagactgt tccgataaag ccatctgctt cattattttt
     6301 gctatataat aatttttgat tcgtatatta agatccattg acaaacggta gctttctaat
     6361 gctgccatca ataaaaacgt acataaaaga aataaaagca agacagaaaa taaaatagag
     6421 cccttataac attttctact gatcgttttt tgtccatatg gcatatccat cttctccatt
     6481 ttcaaagtgt acataaaaag aaattgtgtg ttctttttcc aataattgaa gctcagtgat
     6541 acttgttaac ataggttgat gtccgccgga agttcttctt ttacgtatca ttgcctgata
     6601 tctttcgatg atatactgtt tatcctcgat cgtgtagtgc agttgctgat ttttcacaga
     6661 gacattgtga ctgctcttga gttcttcttc taattgtgct aaaaatatta gccactcttt
     6721 ttgatcttta cgctgcaaaa attcacttac ttggatggtt tgctgaatca aaagattgaa
     6781 tgtgagcaat agactgctca atatcatcag tccaattaga cattcaatca gcgtaaatgc
     6841 tggaattact tgttttttca atactgatcg cttctttctc catattgaca aacacttttt
     6901 cgattactcc attattatcg acaatttgga tatcgtatgg gatcatttct tcccttcgct
     6961 ctggtctttt gtgatacatc cgctgcgtct tgatatcttc ataaatcatt cgaataaata
     7021 gcagttcctc cccacttttc ttttctgcct gaagcaaaaa cagctggcca aagctcacta
     7081 agtagacaca gacagaagcg atcataagcg ctgtaaggga ctccaacaaa acaaatcctg
     7141 ttttattcaa ttctttttgt atagcgacca ctccctatct ggaattgata cgtaatcgtc
     7201 cgctcttttt cttcccaata aaattgataa gcttttagtg aactttcatt cccggttcta
     7261 gcagcaaaag taatagaagc atggcgtttc aaggtaatct catcaggaat atctaatact
     7321 tcccaattta ttttatctgg attcggaaca tgaaaaatga tttgattctc ttcactattc
     7381 caataaaagc ctgtttggat ctgattcaca atagcgattt tttgcgtaga atagattcgt
     7441 ttttcaaatt ggccgaaaaa ttgatagact gccaattcat tcttccatgc agaaaatgcg
     7501 agaactggaa aagaaaggat cgttgtaagt aataaaatca gtaagagtga ttcaaacaac
     7561 gtatatcctg atgctaaaac cttaccttca aggccgctga tagatttctg catgttctcg
     7621 tttgatatag ccttcattca acaattcctc cacagtagga actcttccat tattttgaag
     7681 cgcatagagc tctttttgtg tgtctaccaa ctggatgatc gcttcacgac tttcttggtc
     7741 tacatgattc ctataacgcg acaaattagg gacgaacaac aacactaata cactgatgat
     7801 cagtaagacg attagcattt cgatcaatgt aaaaccgcta taataagata tttttctctt
     7861 tttcatggca aaacctcctc tatgcttccg taaacaggta ataaaacagc ggcataaact
     7921 aataaaatca aagccgctac gcccaaaaaa atgattggct gaataaaatg catccagtga
     7981 tgaagacgtt tgaaaagctg ttttcttgtc cattcgctgt aaaaaatcag ttctttacct
     8041 aaacttccct ttgcctctcc ctgtaaaaca attttactga attcttctga tagaaaacga
     8101 taattctgga actgctcagc caatgacata cctgcctcca atccttgaat caacttagag
     8161 gcaagatgct gaatcagact ttcttgtttc gtttccttta agcagactat gatttgcctt
     8221 aattcgaaac cttcatgaaa aagctttcca atctctagtg acaaataagc cgattgatac
     8281 aaggaatata tcggtccaat caataattgt ctgcttatcc attcactctg atcaacagga
     8341 gacaacttca tcagacgata ccttactagt aaagccaata gagtaaccgc taggatgaat
     8401 agtagaacat accaatgaaa gacttgtatc agctgaatac cccaatgctc ttgcatgacc
     8461 attcctgaac caataagctg aggcaaaacg aacaaccgca tactaaacaa gatacctagc
     8521 aaaaaggtga gcaatagcat cggatatgca agcagctttt tcaaatcatt gcgaaatcct
     8581 tcaacaagcc gaaactgttt ggcaatttcc aacaaagttg tcggcagatt cccatgacgt
     8641 tcagctaact cgatctgtac cagttgctct gctccatatc ctatttgcga aagtacctca
     8701 ctaaacgctt gcccttcctt tagctgatgt tggatatttg ccaaaataac aggtggaaac
     8761 gaacggatcg tgagtaataa ataaagggat tcttgcagac taaaaccaga taaaagcagt
     8821 tctcccatgg tttgtgcaaa ctggttttgc tggcttcttg ttaatccttt agaatagttc
     8881 tgactgccaa atattttcct gagcttcttc ataactttcc tcccatgatt tttttactga
     8941 ttcttcctcc ataaattgat aactccataa aacagaagga tggtgattgg catcttccaa
     9001 aagttcttga taaatgacac cagctaaaca ttcagatagc tcttcttttt tctttgtcag
     9061 ttcatggatt ctgaccaatg taccttcaag attgcgtgca tgtacggttg caaaaacccg
     9121 gtgccctgtt agtgctgctc ttaccgcagc atgtgcagtt gattcatctc ttatctcgcc
     9181 aatgattagc aagtctggac gatgccgtaa tgcgagtctc aaaagtgtat cataagtctg
     9241 tgctattttc ggattgatct gtagctgcag aaaaaacggt tcttctatct ctacaggatc
     9301 ttcgatagta atcacttgca atttttcttc caaagccaac cgatacatta gagaagtctt
     9361 gcctgatccg actggtccgc tgaaaaggta taatccacgt ctgacggttt tctgtaaaat
     9421 agttgcaaaa tctcgagaac taaagcaacg atggggtgtt tcaaaagtac cgtataatag
     9481 ccgaatcact aaactctctc gttgaaggta atctcctaca gaagacaacc gcagacgaac
     9541 ttcctgtttt tctaaagaat aagtaaatgc acctaactga gcttttcttt tttctccgat
     9601 atccatctgt cccaaatact taaatcttga gatcagttgc tgtccatctg ccgaagagac
     9661 tttcttccag aatgttcgtt tttttccata gcgaaaatag atgtaaaata cttctgaagc
     9721 tggcaatata tatacgtctt gggcatgttt ggcaaaactt tcacttatca tttgatccga
     9781 taatcgtttt atttccatta ttctcccctc cacaaaaaac atacgtcttt ttgatctttt
     9841 tgtttcaaat aaaatgaatt tacaaaaaaa tattggcaaa aaccgaattt atgttatact
     9901 tataaaaatt tatcttggga ggcactccat tggaaacaac attaaggaaa tatatcgaag
     9961 tagcaagtaa aaaaaagcct gctgacctag tgatcaagca tgctaagatc gtcaacgtct
    10021 ttacgaaaga aatcatggaa aaagatgtag caatctgcga aggaatgatc gtaggaatcg
    10081 gtgaatacga aggaaaaagc gtacataatg ctaatgggca atatctagtg ccaggattca
    10141 tcgatggcca tgtgcatatc gaaagctcac ttttatcacc aagcgaattt tcaaaagtat
    10201 ccttgcttca tggagttact actgtagtga cagatcccca tgaaatagga aacattgctg
    10261 ggagtacagg tcttgagttt atgatagaag atgcaagaca aacactcatg aatatctttt
    10321 ttatgctacc ttcttgtgta cctgtaaccc cttttgagac caatggtgca aatttaaatg
    10381 caaaaaaatt agcccctttt ttacaaaaac ccgaagtttt aggcttagct gaagtcatga
    10441 actatcaagc agtagcaaat aatgaaacag acatcctaga aaagatccaa ttgatgcacc
    10501 agcataagaa aaaaattgac gggcatgcag ctggtatcgg catggaagag ttaaatgttt
    10561 atcctgcagc aggtattcgg acggatcatg aagctacaac tgcaaaagaa gctaaagaac
    10621 gtttggatct tggtatgtat ttaatggttc gagaagggac cgttgcaaaa gatctggcat
    10681 cgctactacc ggcgatcaca cctgaaaatt caagaagatg cttctttgtc accgatgata
    10741 aactgatcaa tgatttagta aaagaaggat caatcgatca tatcgtccgt caagctattg
    10801 cacttggaat cgatccatta caagccattc agatggcaac attgaatgca gcagaatgtt
    10861 ttgatttgaa aacactaggt gcgattgccc ctgggtatca agctgatttc ttcttaacag
    10921 acgatcttac caagctgcca attcatgaag ttttctataa gggaacatta gttgttcaat
    10981 ctgggcaact ccaagaccat ttatttgctg atcattcaaa ttcatatacg tgttgtctgc
    11041 caaaactaaa cgctcaacca ttaaatccac attcgttaga gctgcttctt tcttcttcac
    11101 aagcccatgt gatcgaaatt atcccgaata gtctagtgac taaagatttg gtagaaaaag
    11161 tggaccgaca aggcgaacga ttcattcctt caatcgaaaa agaccaatta aagattgcgg
    11221 tgatcgagcg tcaccatcaa acgggaaaca ttggattagg catagtgaaa ggatttcagc
    11281 tgaaacaagg agcgatagcg acaacgattg cccatgactc gcataatcta gttgtcgttg
    11341 ggacaaacga cgaggatatg ctgtatgctg ctaatctttt gattcaaaaa ggtggcggga
    11401 tgaccgttgt caacaaacag caggaaatag cttgtgtttc tcttcctata ggcggaatca
    11461 tgacacagga acctttttcg atcgtctatg aacagttgaa tgaattaata actaaagcac
    11521 atcaactagg agcagctaaa acctttgatc cttttttgac actttctttc ttaacattat
    11581 ctgtcatacc cgaattgaag atcaccgaca aaggattatt ttcattttct cgcttccaat
    11641 tgattccacc ttgtggaaaa accgatgaaa taaatacatt ttaaatcaaa taaatcccag
    11701 aaaatacatt acatctgtaa tgttcatttt ctgggattta tttatggata ttaatgtttt
    11761 tatgaaagca gaaagttagt ttcacagact tttctttttg gtcttatctt gaaattgagc
    11821 ttctactcga taaatacgtt gaccttttga agaaaatttt tcttcatact ccgtcataat
    11881 attgccttca aattgacttt gatgtaaatc taaccatacc tgtttgagga tcatgccata
    11941 ttgagaaaaa cttgccagag aatactcaaa caatccttga ttatctgttt tgaaatggat
    12001 ttctccattt ggacgaagaa tctgttcatc tacagcaaga aaagtcttat atgtcaatcg
    12061 ccgtttttca tgacgttttt ttggccatgg atcggaaaaa ttcagataga tttgatctac
    12121 ttcgctgtca gcgaaatact ctgtcaacgc tgatccatcc acatgtagaa gtttcaggtt
    12181 aggaagcggt tgttcgatca acttgtctag tgcaatcgaa acaacactga cctgcatttc
    12241 tactccaata taattgattt caggatgggc ttttgccatt ccagtgatga actggccttt
    12301 ccccatacca atctcaatgt ggattggatg atcgttttca aacaattcat tccattttcc
    12361 cttccacaat gttggatcac taattacaaa attcgggtga gccgctaaca tttccgctgc
    12421 tcccggacga tttcggacac gcattattta tctccattcc tttcctctaa ttaaaaaggt
    12481 ctggccaaat ctccgcagag tttgggccaa acctcagcca ctgaaaattc gttttaattg
    12541 taaaatttct tcgttcatcc gtcgatgatc ttcacgtttg caatatctgg tgatttcttg
    12601 gagcaaattc atggccgcat accaatgaaa tttttcaagc atttcgtcag tcgggcgtat
    12661 gccataactg atcagccact gattccagct agacagtggc acataatttc ctaaaatcgt
    12721 tgcaatatcc accgcagggt ctgcaaacat gattgaatcc caatcgacta gatataaata
    12781 atttttacat actaaccaat tacgatggtt tacatctccg tgtactactg ataagttttc
    12841 cttatctatt gacgggatct gctcacgaag aaaacgatgg acaatttgca gaaattgatt
    12901 tttctggagt tcttccggta attctttttc atatttttgc agcatcttct ccggcgtcat
    12961 gacacgacca ccgatacggg tcagcatggt tttaagagaa tttgaatgat gtaaatggta
    13021 caaaacatca atcacgtcgt ttctttgacc aatctcatta ggagtcagca agcgaccttc
    13081 cagccactct tgtgctgtca aagtatcgcc attccccgtt cgttttgtcc aaacaagttt
    13141 tggtgtaatt ccttcccttg aaagagcagc caacatcgga gtcgtgtttc gtttaataaa
    13201 gactttgtcc tcatcacgca tgcctatgta tgtttttcct gtgtcgcctt tgatgggacg
    13261 taaccgccac tcttggtcta actgaaactc catcatgcat tctcccgtcc tcttactctt
    13321 tgttttgcag tacttattat tctagtcgcc tgaacactac acgtcaagca catatacgtt
    13381 ctttttcatt taattgccat tcaaaaaacc agcttttctc aaaaaaaata gaacgaacag
    13441 cattcttact gtcaacgctc tatttttcat tctcattttt accctacaca gctttgtttt
    13501 cagtctgaca tcttcttcaa acgagttggt acatatagtt taacaaagag catagtaaca
    13561 atcaaataag ccgctagcgg aacccatcgc tgctgcccat ctaatacgac tgcagcaatc
    13621 aaaccaaaaa taaccgacgc gataattaat acccatgtga gtagatactg aatcgcctgc
    13681 ttcttctgat tcttatggat tggataaagc tgaacgagaa ccatatagcg gaactggttc
    13741 gctaaaggta acagttggaa accgatcaaa tataaaaata agaaaccgat aatcagacta
    13801 aaataaaagt cgactactcc ggcaatcaat aacccaccta taattactaa tcggaaataa
    13861 agaccactaa aatcagaacc tcttgccaat cttcttgcaa acaagtaaag ataagtgttt
    13921 tttgattctc ttttaatatg agtcaatata ggatccaaat agcgtcttct tttgatcttt
    13981 gccgtaattt caggaacatc tgtgaataaa ttgataaatt gatagatctg atgcaaccta
    14041 tgttcttcta catgcaccat tttctcccaa tccaatcctt tttcttgttg cttccacaaa
    14101 aaccagtaat atagacttgc ctgtactaat gcacctgcta ttcctgccca tattagccaa
    14161 taaaatgata gaaacaaaaa gatcagagaa gaaaaaaacc agcctaattt ccaataacga
    14221 ataacttgtt gatctagtcg aaacaattct gctctcttga tttgcagatg actgaatttc
    14281 aaactccata atatgatcag atagacgaaa aacatcgaaa aggattgctc cgtactaacc
    14341 accactaatg gcatggccaa tccgcatgcc aatatcaaaa agatcaacgg aaaaatgcaa
    14401 gaatagcgaa aagctcgttc caaataggtt tgcatttctt tttctttagg aagtaaaaaa
    14461 acaatatctg ctgcctctgc aaaagtagcc atcttaccca cgaataaaca gagcatccaa
    14521 aatacagaga ttatgagccc tccaattgca aacggtggct gcagggtttt caaccaatta
    14581 gaataatata atcccacacc gccaatcaag aaaagacaaa ctaaaacaaa atgatcgttc
    14641 aatacataac gcatatattt catcattttc ttttgatgtc ttgctaaacg tttttgaaaa
    14701 aaactactca tgtgcctgct cctttgttaa agcaatatag atttcgtcca aagaagaatc
    14761 cggcagctga aattcttttc tcaacatctc aagagaaccg tctgctcgta tctctccgtt
    14821 atgtaagacc acaaaacgat cacaatattt ttctgcagta gctaaaatat gcgtagacat
    14881 caaaataccg gctccttctt ttttcatttc taccatcaat tcaagcaatg cattgatagc
    14941 tagtggatcc aagcctaaaa atggttcgtc gatgatatat aaagaaggct cgatcaaaaa
    15001 ggcgcataag atcatcactt tctgtttcat tccctttgaa aaattagctg ggaaccagtc
    15061 aagacgatta gataaccgaa aagtctccaa caacttttct gctcttttca ttgctatatc
    15121 cataggtata tcgtaggcca tcgcagtgac ttcgatatgt tctctcaatg tcaattcttc
    15181 atataaagaa ggagtctctg gaatataccc aatttgtttg cgatattctt ccgtattttt
    15241 ctttaaggat agatgattga tttctatcgt tccttttgaa gcttccagca gaccgataat
    15301 gtgcttgatg gtcgtacttt tccctgctcc gttcagtcca atcaacccta ctatctctcc
    15361 aggctgtaca tcgaaactga tatcttttaa cacagggaga tgtccgtacc cccctgtgac
    15421 atgatcgata attaaactca ttctttttcc tccgccattt atttttattt attataccac
    15481 gaagaaaatg acggtatttt gtttttttcc ttacaatagc tttaacttat ggtaaagtta
    15541 agctgaagta tttattcttt atagaaagga tgttttatat ggaaaactgt attttttgca
    15601 aaatcatcaa tcaagaaatc ccaagctata aaatatatga agacgacaag gtctatgctt
    15661 ttttagacat ctctcaagca acgaaaggac acacattagt tgtgcctaaa cgtcatgtag
    15721 cagatatttt tgaatatgat cccgaacttg caggagaggt tttttcgcgt gtgcctaaga
    15781 ttgctcaagc attggaaaaa gcttttccag aaatgaaagg tcttaatatc ttgaataaca
    15841 accgtgaact agcctatcaa tctgtttttc attcacatat ccatttagtc cctcgctaca
    15901 gcaaagagga tgatttttct attcactttg tcaatcatca agatagttat ggatcggaag
    15961 aactaaaagc tattcaagaa acaatcgtaa agcaggtgtc ctgcgatgat taaaaacttt
    16021 ttcaaaggac tgttatttgg agctgctgct ggcactgtcg gaggtctgtt attggcgccc
    16081 aggagcggga atgaaacaag aaagaaactg atcgaagaat tggaagaagc aacaactctg
    16141 acaaatgatt taaataacag cctgaatcat tttaaaagcg cattgatcga aacaaaagaa
    16201 acagctgaat ctgtgattcc tgctttccaa gaagcaatca aaaaagatat cgaagatttt
    16261 aaattccaag ccgaaccacg tatcacacaa atcaatgaac aagtagaaaa actaaaagct
    16321 gatctgccag agtatcctga acaaatataa taatgattat tttctcaaca gatttaacta
    16381 tataagtgag aaaagtttgc gtatttccta ggaatgtgat gaaattttga attttgtttg
    16441 aaactaccac gacaacgttt ttaaaggaaa tgggatatgg tatagttaat atataaaaat
    16501 aataactaat tacaggagtg caaattttaa tgaaaaaaaa atcaattatt ttggcagcta
    16561 ctagtgcgtt ggctgtttta actctagcag cttgttcagg cgatactaat aaagatatcg
    16621 caacgatgaa aggcggaacg atcactgtat ctgatttcta tgatgaagca aaacttgaat
    16681 catcgaacca atccttagta caacggatga tcatttataa agtattcaat aacaaatatg
    16741 gcgataaagt aaccgataag caagtggatg cagaatacga caaacaagcg aaatcattag
    16801 gtgatacttt tgaatcacaa ttagaagctg ctggatatac gaaagatact tataaagaat
    16861 atattcgtaa caatttagct tttgaagctg gcttgaaagc tcacgtagat attacagatg
    16921 acgatttgaa aactgcttgg aaatctttcc atccagaagt agaagcacaa atcatcaaat
    16981 tatcaagtga agatgaagca aaagatgtga aaaaatcagc ggatgatgga gatgatttct
    17041 ctaaattagc caaagacaaa tcaactgata cagagacaaa agaagatggc ggtaaagtta
    17101 aattcgactc aacgacaaca actattcctg cagaagtaaa agaagcagct ttcaaattga
    17161 aagacggtga gatctccgat gttattacta caacaaatcc tacttcttat gcgacagaat
    17221 actacgttgt caaaatggtg aaaaaccaaa ataaaggcaa tgacatggat aaatacaaag
    17281 atcaactaaa agatatcgct actgaaacta aactaagtga caatgcattc acaactaaag
    17341 taatcggtga agaattaaaa gatgcaaatg taaaaatcaa agatgatgcc tttgaaaatg
    17401 tgttgagtgc attcacaaca acttcatcaa gtacgaaaga ctcaagtgaa actacagctt
    17461 caacaaagag ttctgacaca aaatcaactg attcaacaaa agaatcttca actgaagaaa
    17521 caactgattc atcaaaataa aagaaaacaa aaaaagcgaa gacttatgtc tcgctttttt
    17581 tgttttgaat gaaaaaaata aatttttgct ataaatcttt tggtacatag aaacttcggt
    17641 tgtccatacc gaaaatccga tcggtgtatt ttcctggttc tgtttgacga atcgaagtca
    17701 acatcatctg catcgaagca tcgatattgt cgatttgatg taatatttct gcttccatta
    17761 ttcttggacg tacaggtgag ccatattcta ataagccatg gtgtgctaaa accatatgac
    17821 gtaatacgag aacatcttcg tcagcttcgc tgattttcaa cgcaagacag gctttagtta
    17881 tttcttcgtc aatcaatacc agatgaccga tcaaatttcc tgcaagcgta tattctgtcg
    17941 acataggacc ggaaagttca atcacctttc ctaaatcatg caaaatgata ccagcataca
    18001 acaatgctcg attcagctca ggatattcat cggcgattga tttccctaag tgaagcattg
    18061 tcaaagtatg ataagctaat ccactggcaa atgcatgatg gtttcttttt gctgccggaa
    18121 actcaaaaaa ttgtttttga tatttattaa gtagatagcg aacaatacga ttccaatgcg
    18181 cattggtgat ctcaaacaac aattgattga tttcttcttc catttcttct ttcttcaaag
    18241 gggctctttc catgtataaa ctaggctctc ctggctcatc tacccttgtt aagcgcatat
    18301 gaagaatctt tacttgaggg tttccttgat aaacctctct tttaccgttt agaaaaacga
    18361 ctgtgccggc tgtaaattta ttgatttcct cctccgaagc atcccagtat ttaccatcga
    18421 tagtacctga agtatcttgg aaagtaaaag caataaattt ttttccgttt ttagcgattc
    18481 ttacatctgc acttttgatc aggacaaatt gttcaaactg ctcatcgatt actaaatcac
    18541 gaattttttt cattatttgc tccttctaat tgttttacga tttggttcat ctcatggtaa
    18601 tagcttacca tttctttatc agaagaaaaa caaattattt gatacttttc tccaaattta
    18661 gctaaaagtt cagctaattg atactttcgc tgatgatcat aatgaagcca gccatcatca
    18721 atgatgatcg ggcatatcga acggttagct tctaatgaga ggtaagcaaa tctcatcgcc
    18781 ataatcagct gatctttcgt acctgtagat aactcataga tgggaaattc tgtatatccg
    18841 tctgttaaag tcagtgtatc atttttcagt aatatcttct gataatttcc ttttgttaaa
    18901 atacctaaat aatcacctgc tgctcttaac aactggggta attgtctttc actcatctct
    18961 gttgaaagat cacctaagaa tgtcccggcg atttcaagtg ctccccattc ctccagtaat
    19021 tcctgtaatt ttgctttttg ccggctagct tgttgataaa gttcgtccaa tgtgccgtct
    19081 ctttgcaact cttctacttg aagttggagt cgttgccgat tttcacttgc ttctttttct
    19141 tcttgctgga gttgggattg cttttctttc agttggcgca gtcttacaga taatttctct
    19201 tttgtcagct cttcaggaaa tagtggagta agcatttccg ataattcatt gtatcttttc
    19261 atctgctgtt cggcttgttt ggctttttgt aaaaagacag gtacttccga aggataagat
    19321 aagcctgctt gagtcaattg ttcgctgtat tcttgtatcc atactttttg tttctttttc
    19381 aattgattga tttcttgctt caataaagta ttttgctgat agcttctggc aaatttcatc
    19441 tgttccatct cttgtataaa acgggaaagc aataccattt tttctgaaat tgtcttatct
    19501 tgtatcggta accattcttt aacaaactca aatttttggt cgatttgtcg tagtttttct
    19561 tgtatttcct gttctctttt atgtttccgc tcaaatacgc catataattt ctgataatct
    19621 atgacttcct gtccatattc tttcatgagt ttgagcgtat ccatctttcc taagtgccga
    19681 tgatccgcaa aacgatatac ttcttgctga agaatctgtt tttcttctcc agttctcttt
    19741 tgtttttctt ctgcttcagc taattgagct aaggctagat caagttgtcc tagtttttct
    19801 tgccacattc cttttatctc agagaaatca tttttagttt taatttttgg ttgccttttg
    19861 tagaagaaat agccaatcat gcctgcacca attagtaaaa ggatcacttt caagggcatt
    19921 ggtacaaata tacctgcaat gattattgct ccgccagcca tcagcatata cggtttagat
    19981 gaagagactg gtgctatctc tgactgattt tgtaataatg ctggataggt ctcttccaag
    20041 tcattcactt ctgcttcaat catctcttgc tgttctttag ctatttggag acgcacagaa
    20101 atctggcttt ctagatcagc aatccttctt tcttcagcca gaagatgctt ccattcttct
    20161 tcatataaaa attcttccgg taacggattt ttctgccaat gccacctttg ttccaaaatc
    20221 ttcaattctg aatttgtttc tctagtctca atttgtaatc tttgccattc ttcgtttaat
    20281 tgttccgctg taaattgaag ttgctggaga tcttttagtt cctgctcctg ctctaaataa
    20341 aaataatatc tggctgactg ctgatccatc ccgctatgct tttccagtgt tgcttctaat
    20401 tcttccaaac gctcatttgt ttgctgatac cgtttggaga aagactccag atctaaaaga
    20461 agttgctcat caggaagacc tgtagtgctg ttttttttga gagattgcca ttcttcatat
    20521 aaagaaaaat taagttcttg ctgtctggct aatgaaaatt gtttgttcgc ctcttttaat
    20581 tgtgccaata actgctgctg ttgcgactcg tattcctgtt cttgttggat caacttgcca
    20641 aaataagctt cctgttcttg tttttgatga attttttctt gcagctcttt ccattcattt
    20701 aatttttgat tgattagtaa tttttggcct tttttcttga ataattgttg tgctttctga
    20761 taatattctt gccgcttttg gaatacttga gaactgcccg tgatgcctaa agaaatcagc
    20821 gcatcatgta gctctttttc tctcaaatga tctagttcca tcaactgctc ttgctggaaa
    20881 gtaaatacct gctggaataa ctctttcgtc aaaggtgcga tcagtttttc tagtaactca
    20941 tcacttccca cttgatcccc cagccacacc ttcgattttc cgcgattttg ctgcttgtag
    21001 cgctcgacag caaattcacc atatacagga tgtttaagcc atattttgcc gccataggct
    21061 gagccgtctt ttggtgtata atcctttttt ttacggcctt ttgaagggaa accaaataat
    21121 attgcttgaa taaattgata aatcgttgat tttcctgctt cgtttccccc atagatcagc
    21181 tgattgcctg aataaaagtc aaaacttttt tgacgccatt ttccaaaagc agcaatctcg
    21241 atccttaaaa tcttcattcc tgatcctcct caaataaaaa atcttgatta agcagctgct
    21301 tggctttttc aagtgtttct tcttgatatt ccggcagttc gctcaatatt ctagccgctt
    21361 cttgatgact atatacttca tttaaaattt gttggaaatc ttgaggtgtc tgatagtatt
    21421 gaaataactg ttccatcaat ttagcagaag cagcaagtgg ggtcttcgta taaaaagtat
    21481 tctctatgag agaaatacgc caaataaaga aatcatttat cctatctgag aattcctcta
    21541 acaaatagtc taatagctct cctgattgta tccgttccaa aacatctgta ttgagatggt
    21601 cataatcttt taattttatc tcaattaaac taaatccatc ctctactttt gaaagctgct
    21661 gacgaatgtg aacaatgacc tcttttgtcg ttcttgcttt cctcaatgat atttcctttg
    21721 tcttccaata gacctctgct actttaatcg gatgtatttg gcaacttcct ttacttaatt
    21781 caactagtag aatagatgta gaagcttgtt cctttttagt atgaccttga ggcgcacctg
    21841 gataaacaat tgtttgcttt tcatttaaga ccgttggcac atggatatgc cctaaagccc
    21901 aataatcgta tcccttttct tgcatctttg agggttggaa aggagcatag tttccttttt
    21961 gagagacgcc aggttccccg tggtacaacc caatatggta atctgtgagt tctctagaag
    22021 gaaattccat cactttgtct ttttgaatcc acggttgacg ataactaaat ccactgagag
    22081 agacagtttc tccacttttt atcgttattt tttttgtctc taccgtttcg gatgtaaata
    22141 aatggacatt tttaggaaag gagaaccaat accgctcttt ttgatagaaa tcatgatttc
    22201 cgaatattat aaatacaggt atatggttct tttctaaccg tttcatctgt tctgaaaaat
    22261 gtttctgtat ttttaatgaa ggacgatttt gatgaaaatt atctcctgca agtaaaacaa
    22321 aatcaacgtt atttattatt gcctgttcaa tgatatttga taaaacggtc agattcgtct
    22381 tcaatagttt ttcctgtaca gttttatcaa gagttgtcag tccttcaaat gaccgatcca
    22441 tatgtaaatc tgccgcatgg ataaatttca ccattaaaaa tttcctcctt tttttctttt
    22501 atcataccat tttcaatgtt ttttttcatc aataaccgaa aatttgttcg tattatgcaa
    22561 agaaattacg ataaatggcc gacttcaaaa aataagttta ttcgaaaaaa agattgtgac
    22621 atgatttttg tcacaatcct aaatatctat gttatattcg cttcgtttga cactgaagaa
    22681 gtactatttg tactggaatt tatgctccat ttttccgtat ttagaattct atgttctata
    22741 ctaccttaat tagtttatat ggtaagaatt ctttcaaatg cttgagatac tccgtcttta
    22801 tcatttgaaa cagtgacata atcagctttt ttcatgattt cttgatgact atttcccatt
    22861 gcaaaactaa tatctgcact ttcaaacata gataagtcat ttaaactatc tccgatcgcg
    22921 attctcaaag gtttctttaa attgtagatt gattccaaac gatcaaacac cacaccttta
    22981 gatacatttt gaggtacgat ttcaatattt acaggtgccg attcagtaac agacacattt
    23041 agcatctttt tcaaatggtt aaatatatta gctttcaatt gacgattagg aataaaaata
    23101 aaaa
//
LOCUS       DAJSMI010000028        22626 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJSMI010000028 DAJSMI010000000
VERSION     DAJSMI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.028"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     regulatory      60..139
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            137..1330
                     /locus_tag="ON430_000904"
     CDS             137..1330
                     /locus_tag="ON430_000904"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR3218835.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1460..2932
                     /locus_tag="ON430_000905"
     CDS             1460..2932
                     /locus_tag="ON430_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3218836.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3015..3803
                     /locus_tag="ON430_000906"
     CDS             3015..3803
                     /locus_tag="ON430_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR3218837.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3870..4538)
                     /locus_tag="ON430_000907"
     CDS             complement(3870..4538)
                     /locus_tag="ON430_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCR3218838.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGIKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4653..6365
                     /locus_tag="ON430_000908"
     CDS             4653..6365
                     /locus_tag="ON430_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218839.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6368..8137
                     /locus_tag="ON430_000909"
     CDS             6368..8137
                     /locus_tag="ON430_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218840.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8342..8908)
                     /locus_tag="ON430_000910"
     CDS             complement(8342..8908)
                     /locus_tag="ON430_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3218841.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(8908..9864)
                     /locus_tag="ON430_000911"
     CDS             complement(8908..9864)
                     /locus_tag="ON430_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR3218842.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(9864..10520)
                     /locus_tag="ON430_000912"
     CDS             complement(9864..10520)
                     /locus_tag="ON430_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR3218843.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            10890..12908
                     /locus_tag="ON430_000913"
     CDS             10890..12908
                     /locus_tag="ON430_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3218844.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFSARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    12978..13184
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13335..15749
                     /locus_tag="ON430_000914"
     CDS             13335..15749
                     /locus_tag="ON430_000914"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR3218845.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16097..17650
                     /locus_tag="ON430_000915"
     CDS             16097..17650
                     /locus_tag="ON430_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR3218846.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18031..19815)
                     /locus_tag="ON430_000916"
     CDS             complement(18031..19815)
                     /locus_tag="ON430_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218847.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(19830..21551)
                     /locus_tag="ON430_000917"
     CDS             complement(19830..21551)
                     /locus_tag="ON430_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218848.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            21651..22412
                     /locus_tag="ON430_000918"
     CDS             21651..22412
                     /locus_tag="ON430_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3218849.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7191 a         4066 c         4681 g         6688 t
ORIGIN      
        1 ttttttaagc aaaaaaatga aaaaatacga gcagaaaact gttttatctg ctatacttgg
       61 ttcgttgcaa gttcccgaaa ggatccgcag gacggagatg ccttgtaacc gaatttaact
      121 agggggaatc attataatgg tagaaagaca cttatttaca tcagaatctg tttctgaagg
      181 acatccagat aaaattgctg accaaatcag tgatgcaatc ttggatgcaa ttttaaaaca
      241 agatccaaca gcacgagtag cgtgtgaaac gtctgtaaca actggtcttg ttttagtatt
      301 tggagaaata tctacgacgg catatgtaga tattcaaaaa gtcgtacggg aaacaataaa
      361 agaaattggt tatacacgtg caaaattcgg atttgatgga gatacagcag ctgtattggt
      421 tgcgattgat gaacaatctc ctgatattgc tcaaggagtt gatgaagcac ttgagatccg
      481 ggatgaagat aaaaaagacg tattagatga gattggtgct ggtgaccaag gtttaatgtt
      541 tgggtttgct gtagatgaaa caccagaatt gatgccttta ccaatcgctt tgagtcatcg
      601 tctggtacga cgcttggcag atttgcgtaa atcaaatgaa ttaacgtatt tacgtccaga
      661 tgcaaaatct caagtaacgg ttgaatatga tgatcaagga caaccggaac gcgtagatac
      721 aatcgttatt tcaacacagc atgatgatgc agtagataat gaaacaattc gtcatgatgt
      781 catcgaaaaa gtagtgaagg aagttattcc agctgaatta ttagatgatc aaacgaaata
      841 ttatatcaat ccgactggcc gatttgtcat tggtggtcct caaggggatg ccggattaac
      901 aggaagaaaa atcattgttg atacgtatgg cggttatgct cgtcatggtg gcggtgcttt
      961 ttctggtaaa gatgcgacaa aagttgaccg ttctgccagc tatgctgcac gttatattgc
     1021 taaaaacatt gttgcagcag ggcttgctcg caaagcagaa gtacagctag cttatgcgat
     1081 tggtgttgct caacctgttt cgatctcaat caatactttc ggtactggaa cggttccgga
     1141 agaagaactg attgctgcag tgagagaaaa cttcgatctt cggccagcag gaatcatcga
     1201 gatgcttgat ttgcgtcgtc cgatttacaa acaaacagct gcttacggtc attttggtcg
     1261 tacagatgta gatttgcctt gggaacatac ggacaaagta gatgcgttga aagcaagttt
     1321 agcaaaataa gttatattca gctggaacga tcgccaagtg gtggttgttc cagctttttt
     1381 tatggcagtt aacctcttga aaaagatagt aattatttat ctatttttag atcctgtcat
     1441 aatgaaaggg aggaataaga tgaaaaaaga aacaaatgtt actttggtca cagtgagtat
     1501 tttcattgca acgttcatga cggctattga aggtacaatc gtaacaaccg ctatgccaac
     1561 gattgtcggt tcattacatg gaatggaaat catgaactgg gtattttcga tttatttgct
     1621 gacaaacgct atgcttactc cgatttacgg aaaacttgcg gataaagtgg gacggaaacc
     1681 aatctttatt tttgggacat tattgtttat tatcggttcg ttattttgcg gtttatctca
     1741 aagcatgatt gggttgattg ctgctcgtgc gattcaaggg atgggtgctg gggcaatgat
     1801 gccagtagct ttgacgatca tcggcgatct ttattctatt gaaaagcgag caaaagtttt
     1861 aggtttgaat agttcagcct gggggattgc cagtgttttt ggaccgcttg cgggaggatt
     1921 catcgttgaa acgatcagct ggcactggat cttttttatc aatgtgccga tcggaattct
     1981 attaattatt ttgatttggc tatatttaaa tgaaccaaaa atcatacatg agtctaaacc
     2041 aatggatatt tttggtagtt ttagtttgat ggccatgctt gtctcattgc tgttagggtt
     2101 ccaattatta ggagacgaag gaatgagtcc gcttgtttct gctttgctgc taggtagtat
     2161 cgtaatgtta ggtatttttg tttttgccga aaaaagagct aaagatcctg tcatcatgat
     2221 gcatttattc aaaaatccac tatttgttgt ggtcaacttg gctgcagcat tggctagtgg
     2281 ttttttgatg ggaatcgatg tttatatccc tatgtggatg caaggtgtat tagggctgaa
     2341 tgcagcaatt ggtgggctgg tattagcacc attatcacta gtctggatgg tcggttcttt
     2401 ccttgcaagc aagtggatga ctcaaatgaa aactcattgg gtcttgagaa ttggactact
     2461 aatcgcagct attgggggcg cttggttgta tcttatgcct ttcagaagta actatttatg
     2521 gttttttgct atatcaacgg tcatcggtat aggcttaggt gtgaccatga ctacctcgac
     2581 tgtcagtgca caaaatagtg tatctcgtga ccagcttggt gtagcaactt cttttaatac
     2641 gttagttcga acaattggtc aaacagtcat ggtttctatt tttggtttat tgataaatgg
     2701 ggtaactaaa cgagaacttg cagcagcaaa tttaacaaat gatgcagata tcatgaataa
     2761 actggtgaat ccacaaacgg ctaaatatat tgatgcggat attttgttac cattaagaaa
     2821 aattctttat gatgggttac attccgttta tctagttggc ttgttactcg tgatcattgc
     2881 tttggtctta acgcttttcg ttcatgaaaa aagtggtaaa ataaaacagt agtttcggtt
     2941 aagatatgta cgtagccgaa tcattcaaat ttaaagttat tcaaaaataa cttcttacta
     3001 aggaaggcga aaatatggca tttattcaag caaatattta ttcgaatgtg ttagaaatgg
     3061 aagtcaatct aaatgtgata ctaccgcaaa caacaaaaaa ggtgattggc accagttcag
     3121 agcaagagac aacggatata cctgttttat atttacttca tggaatgggt ggcaatcata
     3181 gtgtatggga aagacgtacg tcgattgaac gttatgtagc aaactacgga ttagctgtca
     3241 tcatgccatc tactgatctt ggctggtaca cagatacagt ttatgacatg aagtattgga
     3301 cattcattgc agaagagcta cctgagattt gtcatgaatt attcccgcaa ttaacaagaa
     3361 aaagagaaaa aacatatgcc gctggattat cgatgggcgg atatggcgcg ttaaaactag
     3421 gccttgcaaa atcagaaaat ttcgcagcag taatctctct ttcaggcgct gtttctgtag
     3481 gaaatcgaat ggatgatctt ttgatggttc gtaaagcatc tttttgggaa gggatctttg
     3541 gaccattgga tcaaatcgaa ggttcaaaaa atgatccgca atacttattg aaagaactag
     3601 taaaaagcgg aaaaccagtt ccaagatttt atatggcttg tggggaatct gattttcttt
     3661 atcaggcaaa tcaggaaatg gtatctgcta tgcaggaaca aggcttggcg gtcacctttg
     3721 aacaaggacc tggcgatcat aactggatct tctgggacca atggattcaa cgtgcattgg
     3781 cgtggctaat ggaagaaaaa taaaaaaaga gtcttaaaaa gatagctcct atagagatca
     3841 caaacatagt gatctctata ggagcttttt tactgctcgt tttctcttct tttattttgt
     3901 tggcataagc cttcaatata ggtttgtgca ctcgttagaa agagtgcttt ttcattcaat
     3961 tctgcatggg actgcatact ttctttaatt ccctctaatt gattttcaac tacataatta
     4021 cgcataaata gaactgtttc tttcaaaaat tcatttccgt ccataatttt ttgtcgaagc
     4081 tgctgttcat gctgctgatc cattaactgt ccaatcaata aattgtggag agaggcaatg
     4141 gcctgatgac tttgggtgac tgaaaatcct tcacgaacaa gtaattggat catttgctcc
     4201 aaatgttcta atcgactcgg aaaagcgggc acagtattga tttctaattc tgccccacaa
     4261 gggaaacgtg tataaaggtc atagtaattt tccatatact gcagcaactg ttcattccaa
     4321 ggcaaagttg gatctggaag cgctaattct tcttccattg tttcagccat tttttgcaag
     4381 agctgttgtt tgtttttgaa ataccaatat aaagcaggtg cttgaacatg aattctgttt
     4441 gcgatgtttc gcatggaaag ctgcgaaaga gcttgtgttt cagccaggat atcaaaggcg
     4501 gcctgaatga tgattgcttg agaaagcttt atttccattt tatcaacctc gtgtattgca
     4561 ttatcttttt aaacatggta aattgttagt cggtctaatt ttacagtgtt aaattagtcc
     4621 tgtcaattta aacaaagggg tgtaatcaac gaatgaaatt aatcttacga catgcgaaaa
     4681 aatataaagt agctgttttt atttccttgc tgtccgtagc agtcatggtt gcagcagcat
     4741 tatggcaacc taagttacta cagcaagtat tggaagctat tattacagaa gataatgatg
     4801 aaatgaaaac aattggtatt tacctgatca gtctggcttt gctaggatta gccgcaggtg
     4861 taacgaacac gattttctct gcaaaagtag ctcaaggcgt aagtgcagat atccgagaag
     4921 aagcatttcg aaagatccag acattttcat ttggaaatat cgaacagttc tctgctggaa
     4981 atcttgttgt tcgtctaaca aatgatatca cgcaaatcca aaatttagtg atgatttctt
     5041 tgcaatcact ttttcgaatt ccattcttat ttattggtgc cttcatttta gctatgatca
     5101 cgatgccgca attatggtgg atcatcgtag cgcttatcgt tacggtattt ctgattacgg
     5161 cactttcatt tacaaggatg ggcaagcact tcatgatcat tcaaaaactg attgatcgtg
     5221 tgaatagcat tgcaaaagaa aacttactag ggattcgtgt agtgaaatct tttgtacaag
     5281 aagaaaacca attgaagtca ttcagcaaag taagtgagga cttaacaaaa cacaacatca
     5341 tagtcggtac attattttct gtgatgatcc cgtccttcat gctggctgcc aatttggctg
     5401 tggttggggc gatctttttt gtgagcgatc ttgttaaaga tgatcctacg ttaattggtg
     5461 gaattgcttc attcatgaac tacttgatgc agattatgat ggcaatcatt attggcggaa
     5521 tgatgatgat gatgacttca agagcagccg tatcgctgaa gcggattgcg gaaattttag
     5581 atgctgaacc agatcttact tatttagacg ttccagaaca agagttgacg ggttctgtca
     5641 cttttgacca tgtttctttt cgttatcctg gtgaggatac agatacgctt aaagatatca
     5701 gcttttctat caaacctgga gaaatggttg gtatcgtcgg ggcgactggt gccgggaagt
     5761 caacactagc acaattgatc cctcgtctat ttgatccgac agaaggcaaa gttgaagtag
     5821 gtggtgtaga tcttcgtcaa gtaaatgaaa agagtttaag acaaacggtt tcctttgttt
     5881 tgcaaaaagc aattttattc tcaggaacga ttgctcaaaa cttgcgccaa gggaaaaaga
     5941 atgcaacaga aaaagagatg gaacatgctt cttctattgc tcaagcaaaa gaattcatcg
     6001 aaaagttatc tgatcgttac gaagcaccaa tagaagaacg tagcagtaac ttttctggtg
     6061 gtcaaaagca acgtttatcc attactcgtg gcgttatcgg taatccaaag atcttgatcc
     6121 tagatgacag tacgagtgca ctggatgctc gctctgaaaa gctggtccga gaagcactcg
     6181 atcgagattt gaagggaaca acgacgattg tcatcgcaca aaaaattgct tctgttgtca
     6241 aagcagaccg catcttagta ttggatgaag gcagattagt cggcgaagga acccatgagg
     6301 aattggtagc tggaaaccgt gtctatcagg aaatctttga aactcaaaaa ggaacggagg
     6361 agtaagtatg actgatctaa taaaagcaag taaatttttc tatcactatt tgaagcggta
     6421 caaactctcc tttttcttta tctttatcac agtgatcatt gctacctatc tacaagtaaa
     6481 ggcacctcaa tacatcgggg aagcttttca agaattagct aattacgtag gaggcttgat
     6541 gcaaggcgta gacgataaaa gcaaatttgt ttctgtaata tggaaactct tgctgtttta
     6601 tgtactagct agtgcagcca actttatcta tagtattttg ttcacacaag tagtagggaa
     6661 gtcaacgaac cgaatgcgta tcggtctatt caacaaattg gaaaaattga cgatccgttt
     6721 ctttgattct catcaagacg gtgaaatttt aagtcgtttt accagtgact tagataatat
     6781 tcaaaatagt ttgaaccaag ctttgcttca agtcatcacg aatgctgttt tacttgtcgg
     6841 gatcttgatc atgatgttcc gccaaaatgt agagttagct tgggctacaa ttgcttccac
     6901 tccagtagcc gttttgatag ctgtcttgat tatccgcaaa gcacggaaat atgtggatct
     6961 tcagcaagat gaagtgggga aactaaatgg ttatatggat gaaaaaatca gcggacaacg
     7021 tgtcatcatc acaaatggct tacaagaaga aacgattgaa ggattcttag agcataatca
     7081 aagtgttcgc gaagcaacct tcaaaggaca agcttattct ggtctactat ttccgatgat
     7141 gcaaggaatg tcactggtca atacagcgat agttatcttt ttcggcggct ggttagcatt
     7201 aaatggtgat ttggaacgtt cggtggcatt aggcttagtt gtgacattcg tccagtattc
     7261 acaacaatat taccaaccac tgatgcagat ttcatctgga tacagcatga tccaattagc
     7321 gattacaggg gcaagacgat tgaatgaaat atttgatgaa aaagatgaag tcaaaccaga
     7381 aaacggtaaa gcatttgatg gtgttgaaaa aggtcttgcg ttgcagcacg tagattttgg
     7441 ttattctcca gaaaaactag tcttgaaaga tgtctcgatc aatgtggaca aaggagaaat
     7501 ggttgctctt gttggtccaa caggatcggg gaaaacgacg atcatgaatc tgctaaaccg
     7561 tttttacgat gtcaatgatg gttcagtagt catcgatgga atagatatcc gagattatga
     7621 tcttgatgca ttacgtagac atgtaggaat cgttttgcaa gaatctgtat tgttctccgg
     7681 aacgatcaaa gacaatatca cattcggcaa accggacgct tcagatgaag aagtcatcaa
     7741 tgcagcaaaa caagcgaata ttcatgactt catcatgacg ttagaaaaag gttatgatac
     7801 agagatatcc gaagaaaata acttattcag tacaggtcaa aagcagttga tcagtattgc
     7861 tcgaacgatc atcacaaatc catctttatt gattttggat gaagcaacaa gtaatgtaga
     7921 cacggtaaca gaagcaagga tccaaaaagc aatggatgaa gcaatcaaag gaagaaccag
     7981 ctttgtgatt gctcaccggc tgaaaacaat cttgaatgca gatagaatcg ttgtcttgcg
     8041 tgatggggaa gtcattgaag aaggcagtca tcacgagtta ttagcagaaa atggatttta
     8101 tgcggaactt tatcataatc aatttgtatt tgaataaaga ggttgtgaaa aagctgtcct
     8161 gcatcaagta ataagaacag ccaatcaaaa acagcttctc atgtttttga tttggttgga
     8221 cttattaccg cagatataag cttttgagca ccgtgaataa agaggttatg aaatcaacgc
     8281 tctaacctga gtattagaga aaaaaacgca aaacatatca tttatgtttt gcgtttttta
     8341 tttatctttt tttctcaata caatataaaa taggcggctg gttcttttgg ttgataaacc
     8401 gataattcag cacattatat gtttcctgtg gcagtttgga acaaaaagaa tcaaccatat
     8461 ccagttcttt ttctccacct tcatgaccat agtagacgac aaggatcaat cgtccgcgag
     8521 gcacaagtcg tttcaatatt tcttccatag ctgtgcgtgt agtctctggc atagtgatga
     8581 ttgctttatc actttttggg agatacccta gattgaaaat ccctgctttg agattttgtt
     8641 gttcagctat cacttctcct aacgtttcgt ggccttgtag aaacaatgag acacgttctt
     8701 ctaactgacg ctgttctagc ctttcacgag tagcatcgat tgcttgttgc tggatatcaa
     8761 aactatagac atgaccagtt tttccgatgt gttctgctag aaaaaccgta tcgtggccat
     8821 tgcccatcgt tgcatctacg acgtgatccc ctggttgcaa gatttcttgc agtaatgtat
     8881 ggctgaaatg caaagcagtt tgcaacatca gacgctcacc tttttccgta catcatattt
     8941 tccttggaag ctatctctgc gaagaagttc ttcatcaatt gcattcaaga cttcccattt
     9001 tttcaagctc cacattggtc caatcaagga atcccacggc gcatcccctg tcaaacgatg
     9061 gatgatgatc tcttttggta tcatttccag ctggtcgcaa atgacattga catagtcagg
     9121 acggctcatt aattgaaggc gaccttcatg atagtcccgc aacattctcg ttttgcgcat
     9181 cagatgcatc aaatggagct tgattccttg gatatctgaa tctaaaatgg tgcgtctgac
     9241 attttctttc atcatttcta aactttcgcc tggtaatccg ttgatcaaat gcgtacaaac
     9301 acggatatta tgtttgcgta atttagccac gccatctaaa taagtttgat aatcatgtgc
     9361 tcggttgatc agttttgaag tttcttcaaa agttgtttgc aatccaagtt ctacccataa
     9421 atacatccgc tcgttcagtt cagcaaggta atcaactact tcatctggca gacaatccgg
     9481 tcgtgtaccg actgataatc ctacgacacc tggtaaattg actacttgct caaatcgttc
     9541 tttgattatt tcaactggcg catgggtgtt tgtaaaattt tggaaataaa cgatgtattg
     9601 ttggacatga ggccattttt gatgcatcat gtcgatttct ttctggaatt ggattggaag
     9661 agggtcttca ggtgcaacga tcatgtctcc cgaaccagat acactgcaaa aagtacaacc
     9721 gccatgggca acagtcccat ctcgattcgg acagtcaaat cctccatcga tcggcacctt
     9781 gaaaattttc ccgccaaact catttcgtaa agcgtaattc caagaatgat atcttttatt
     9841 tccttctgaa taaggaaaag tcattaaaag tccttctttc tgtaagagtt atctatttgt
     9901 tttctttttt ctctatacac tggataagtc aggcacagcc aagaaagact gagtaagtag
     9961 ccgccgatga tatcagtagg aaaatgaacg cccagataaa tgcggcttat cccgacactt
    10021 actataataa acatcatcag caattgaagc aatgtgcgta aggttctttt ttgaacaaaa
    10081 gctggaagca gaaatagtaa agaaccgtaa agaatcatac tgcctgctgc atgtccacta
    10141 gggaaactgt aacttgtttc aactactaag tgcgccaatg aaggacgctc tcgcatgaaa
    10201 aatagtttga tcaccgggtt gatgattcca gcaattacaa taaatccaga aacgaaccaa
    10261 aatgtttctg cccttttttt cttataccac atccataaag ccgcacaagc aaataaaatg
    10321 atgacggtaa gaggattacc gaatttagtc ataaagagtt ggtagctgtt cagatcagga
    10381 taagaagctc tgacaatgcc tgtaaaccac tgatcgaaac cagaaagcca cgacagatgg
    10441 aactttacga tccagcccaa aatgaaaaac aggacaagaa agaagctacc agccacctga
    10501 aatgatgttt tatatttcat acaattcctc ctttgaagca atgacttatt atagcatttt
    10561 tggtccagcg aaaaaaacgg taaagagata aaaccaatgt atgattcctc tttgaataac
    10621 caatattctt ataaaaaaaa taactgtaat cggaaagaca ttttttggta atgcaaatga
    10681 aatgttagtg aaataaaatc agtgtttttc gaaatccttt taaacaagaa cgtagacgta
    10741 ggaagaactt gaaaatgaac agaagttgtt cattacggta aattacaata tgattacaaa
    10801 aaacaacaaa gctcttaaat aacttttaat aaatattaag agaagttata attattaata
    10861 atagataaat tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa gaacggagac
    10921 gtttagaaca gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc gtgggaactg
    10981 cgatggtagg ctgttctgtc gctgccccat tagtacagcc ggttcctgtt caagcggatg
    11041 aaacacctgt tcagttcagc gcaagaatca atacagcggc atttatcaca gaaattgcta
    11101 cttatgctca accaatcgct caagccaacg atctatatgc atcagtgatg atcgcacaag
    11161 cagttgtaga aagcggttgg ggcagtagtg cgttatctca agcaccttat tataatcttt
    11221 ttgggataaa aggcagctat caaggacaaa ccgtctacat ggatacgctt gaatttttaa
    11281 ataatcaatg ggtaaataaa aaggagcctt tccgacaata tccttctttt gctgaatctt
    11341 tcaatgacaa tgcctatgta cttagaaata cttcatttgg caacggttat tactatgccg
    11401 gtacttggaa gagcaacacg aaatcctata cagatgcgac agcttgtttg acagggcgtt
    11461 atgcgactga tcctggttac gcaggcaaat taaataatat catcgcaact tacggattga
    11521 cccaatatga tacaccagct agcggaaacg caggcggtgg agcgactaca ggtggcaata
    11581 atactggaag tgcaggtagt actggcggtt caagcaattc tggtaataca aactcaggtt
    11641 cagcaacttc gaccacttat actgtccaat ctggtgattc tgtttgggga atctcaaata
    11701 aattcggtat cacaatggat caattgatcc aatggaacaa catccaaaac aatttcattt
    11761 atccaggaca aaaactgaca atcaaaggta gccaggaaaa tggctcttcg acgaataatt
    11821 ccggtaacaa taccaattct tcaggaaatg ctggtacatc aaacggagga caaacaactg
    11881 gtgcgaaata cactgttcaa tctggtgatt ctgtttggaa gatctcaaat gatcacggaa
    11941 ttacgatggc tcaattgatt gaatggaaca atatcaagaa caatttcgtt tatccaggac
    12001 aacagctaat cgtatcaaat ggcggttctt ctaatgctgg agcaaatact ggctcaaata
    12061 ctgggaatac aagtggctct agttcgtcca gctcaagttc ttctaataca gcttcaggaa
    12121 cgaaatatac agttaaagcc ggagactctg tttggagcgt ctcaaataaa tacggaatta
    12181 cgatggctca gctaatccaa tggaacaata tccaaaataa ctttatctac ccaggacaac
    12241 aactgactgt ttcaaacagt ggatcaacaa acagcacgtc aggtaataca ggttcgtcaa
    12301 ccaatacaaa taacagcagt tcagttaata ctggcagcag ttcgtctcaa ggcacttata
    12361 ccgtgaaagc aggtgaatct gtttggagcg tggcaaataa aaacggcatc acgatggatc
    12421 agttaatcga atggaataat atcaaaaaca acttcattta tccaggacag caattgatcg
    12481 ttaaaggtgg aacttcggca aatacaaata caggttcaac tacttctgca gcgaaaccaa
    12541 atacaccgaa tacgtctgct acgacaagta catcatccac tggtaatacg atgtataccg
    12601 tgaaagcggg cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg atggatcagt
    12661 taatcgaatg gaataatatc aaaaataatt tcatctatcc aggacagcaa gtgattgtga
    12721 agaaaggttc atctcaatca acaaaccaac aatcagttac tggttcgaaa acctatactg
    12781 tgaaatctgg agaatctgtt tggagtgttg ctgacagtca tggaattaca atggctcaac
    12841 taatcgaatg gaacaatatt aagaacaact ttatttatcc aggtcaaacg ttgattgtag
    12901 ctaaataaaa tagctgttcg aagaaataac gattgaattc cacaaaaata tttgttacta
    12961 taaggctact taaatatgac aacacttgaa aaggaagtag taaaaagact gaagtttcag
    13021 agagcgcatg gctggtgaga atgtgcacgg atgcttttga actcgccttt gagttgccct
    13081 tgctgaatac ccgagtaagc aagcggcggt gacggccgtt acaacgttcg aggccttttc
    13141 actaaggtaa aattaggtgg taccgcgtag attacgtcct ataaaaagag caatcttttt
    13201 ataggatttt tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg aaataaaaat
    13261 aaacagattc tgccggtttt ttcgttatat ttataataga aacaagtgtt ttcaatagaa
    13321 caggaggaca gcaaatgagt tacaatcata aagaaattga gaaaaaatgg caaaaatact
    13381 gggcaaaaac gaatactttc aacacccatg atgatcctga aaagccaaaa ttttacgcac
    13441 tggatatgtt tccctatcca tccggtcaag ggctacatgt agggcatcct gaaggttata
    13501 cggcaacgga tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg ctgcatccaa
    13561 tggggtggga tgcattcggt ctgcctgctg aacaatatgc gctagataca ggaaacgatc
    13621 cagctgagtt tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat tctttaggat
    13681 ttagttatga ctggaatcgc gaagtgaata caactgatcc ggagtattat aaatggacac
    13741 aatggatctt tacgaagctt tatgaaaaag gtctagctta tgaagcagaa gtagcggtaa
    13801 actgggtacc tgaattagga acagttattt caaatgaaga agtgatcgat gggaaaagtg
    13861 aacgtggtgg ctatgatgta gtcagaaaac caatgcgcca atggatgctg aaaatcactg
    13921 cttatgctga tcgcttattg gatgatctag aattagttga ttggccagaa agcattaaag
    13981 aaatgcaaag aaattggatc ggtcgttcag taggagcaaa tattgaattt aaagtggctg
    14041 gtacggataa atcctatacc gtatttacga cacgaccaga tactctattc ggtgcaactt
    14101 actcggttct tgcacctgaa ctagatcttg ttagagaaat tacgacccct gaacaaaaag
    14161 ctgctgtaga agcttatatc gaagagacag ctaaaaaatc agatctaaag agaacggatc
    14221 ttgcaaaaga aaaaacagga gtatttactg gtgcttatgc aatcaatcca gttaatggca
    14281 aggaaatccc aatctggatc gccgattatg tattatcttc atacggcaca ggagccatca
    14341 tggctgttcc agcacatgat gaacgtgact ttgaattcgc tcaaactttc ggactagaaa
    14401 ttcttccagt tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa gatggtacgc
    14461 acatcaactc tgaatttcta gacggcatga acaaacaaga agcaatcgat aaaatgaatg
    14521 aatggctaga agaaaatggc gtagggaaaa aagaagtcag ctatcgctta cgtgactggc
    14581 tattctcccg tcaacgttac tggggtgaac caatcccaat catccattgg gaggacggaa
    14641 cagtcacagc tgtcccagaa gaagagctac cattacggtt gcctaagaca aaaaacatca
    14701 agccaagcgg tactggagaa tctccattag caaatatcga ggagtgggtc aatgtcgttg
    14761 atcctgtaac aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa tgggcaggaa
    14821 gctcatggta ttacctgcgt tatatcgacc ctcataacaa aaaagaacta gcagattatg
    14881 aaaagttgaa gcgttggcta ccagttgaca tctacattgg tggagcagaa catgcggtac
    14941 ttcatttact atatgcacga ttctggcata aattcttata cgatatcggt gttgttccta
    15001 caaaagaacc attccaaaaa ctatacaatc aaggaatgat cttaggcgaa aacaacgaaa
    15061 aaatgtcaaa atctcgtggt aatgtagtca atcctgatga tgtggtcgaa acttacggtg
    15121 cagatacact gcgcatgtac gaaatgttca tggggccatt agatgcttcg attgcttgga
    15181 gcgaaaatgg cttagaagga agccgtaaat tcttggatcg tgtatggcgt ttgatcgttg
    15241 atgagaacaa taaaatgcgt gaccgtatca ctacattgaa tgatggcaag cttgataaag
    15301 tgtatcatca aacagtgaaa aaagtgacag aagactatga aaatcttcat ttcaatacag
    15361 caatctccca attgatggtc tttatcaatg aagcctataa agtggacgct ctgccttatg
    15421 aatatatcga aggatttgtt caactactcg caccgattgc tcctcacatg ggtgaagaac
    15481 tatgggcaat ccttggaaat gatggtggta tttcttacgc accatggcca acatacgatg
    15541 aagcagcact tgttgaagac gaagtagaag ttgttttcca agtaaacggt aaagttcgtg
    15601 cgaaaagcaa tgttccccga gacttaggaa aagatgagct agaaaaagca gcattagcca
    15661 atgaaatagt gcaagaatat attgaaggca aaacagtcag aaaagttatt gcggtaccaa
    15721 acaagttagt caatatcgtt gctaattaat tggctctttg tcaataagga ctgatgagtt
    15781 gtgcaaaatc aaatctgggc cagaatgaac ttcattctgt ctcagatttt ttgttatttc
    15841 aaattgatta attgattgat caaaaaggtt ttgattttca tgttctcaaa cacgttcaat
    15901 gtctttttct tctgaatggt cttgtaagag atctctgtaa tcatcaaatt tttgtctatt
    15961 aatcttagta ttttttgatg tataaactac ctgaaattga gagtacatac caaattcata
    16021 aattttttat tagtgggact agtgattgta tggattttga cgcataatga atgaagtatg
    16081 agaaaaggag agacaaatgg atcaagaact atttattatc gaagtattat cagctaaaca
    16141 aaaaattatc gagaaagcga aaaaaagagc gaaaagaata atgttcaagc ttttaagctt
    16201 agtatttttc tctggtttgc tttttttcgg agctgtatcg ttcctatatc gccatatgag
    16261 tgcagtagtc cgttcagctg aagggatgca aaaattaaat aatttgtatc acttttcgtg
    16321 gggagttggc ggaataacaa ttgttgtgct tattgttttt cttctactta tttggctccg
    16381 ttaccatcaa ttgtcgcgtc atttcgatca atggcttagt caaagagcac aacagttttt
    16441 acataataaa ccaacttctc aagatgatga ctatctttat tttgagaaga cttcgacgag
    16501 tcaagctttt cggatacaaa aaagtgattg ccagttattg ctgagatcac ttgatgattt
    16561 acctatctac ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc atattttttt
    16621 actagaaaac gaacccagat gggaaaaaag ccagtcttcg cctggtggat acctgacaac
    16681 taagatgagt acattagtca ctggtttttt cctcattctt ttagctggaa caatctatta
    16741 tgggagatac actcagccaa aaatgtcttc tcatataaat gctgcttctc acatgacaat
    16801 gaactcaact aacgatacgc atatacaaaa gacaaatgaa gggttgttag ctgatcctaa
    16861 gcaaaaggta aatttgacaa cacaaaagat aaatgatctc aaacttgata cagagacgca
    16921 ctctctctat atgacaacag atagtggtgc taattgggta tttgtgccga ttgattcgca
    16981 atggttgcgt tttggcgaat acaccctgac aacggggctg attcctgaag gttattggat
    17041 ggacaacaca tatgatattt ccgccgattt ctcatggttt atctattctt ctggggatga
    17101 tgtacgtctg ctgacttcaa gagataatgg taaaacatgg caaacgtcta ttatcaccca
    17161 atcagttcag ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg gtctatttat
    17221 ctgtacatca gccagtggga tgtctgcaga aactttgtac atttacacta ctatggatgg
    17281 cggaatcact tggataaaaa gtggaagtac aatgatcaat cagccagtac aaaatgtcag
    17341 cttcgttaca acttctttag gttttgtttc gacacgtgaa agtttgtttt atacaaacaa
    17401 cggaggacgc tcattcaaag aatcagttct gtcgattccg gaaaactatc agacaggggg
    17461 gatagatctt tttcaatcac caaatgaagt tactcaaata agcgcgaata cgttagagac
    17521 aaaattttat ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg cctgcctctt
    17581 ccgttcaact gataacgggg aaacatggca attcgttcag caactatcgc aagtagaaca
    17641 aggagattaa agaagagaga ttgtgagcct gacgttcagc accgagtcac aagaaacaaa
    17701 ttcaaaaaac agcttctcat atttgttgaa tagtgtaact cgtgattcgg agttgacaag
    17761 agcaacaaga agcgattttg aagtatagct tctcgtattt tacaagatcg tgtcttattg
    17821 ccgataggtt gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac aagtcttttg
    17881 tcactactta ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag tattattctt
    17941 tgtgtttcta gtggcaccac tagtacattt aaaaaagcta tcgatcatgg actaatcata
    18001 catgatcgat agctttttag ataatgtttg ttacgctatc tgaaattgac tattataaag
    18061 actggcataa agcgttggtg attggagtag ttcagaatgt gttccttttt cgacgatatc
    18121 gccattcttc attacaagga tcatgtctgc attttcaatc gttgacaaac ggtgtgcaat
    18181 cacaaaactt gtcctatctt ttgtcacagc ttccattgct tgctgaatca atgcttctgt
    18241 tcgtgtatct acgctagaag ttgcttcatc taaaataacg acaggaggat tcgctagaat
    18301 gactcgagca attgtcaata attgttgctg tccttgagat aacgtgccgt tttcgctaga
    18361 aatgatcgta tcatatccgt ctggcagtgt tcgaataaag tgatcacatt gtgcgatttc
    18421 agccgcttcg ataatttgtt ctcttgtggc atccattttc ccgtaggcga tattatcagc
    18481 taccgttcct tcaaacagcc aggtattttg taaaaccatc ccaaaagaac gacgtagttc
    18541 tccacgtgaa agcttggtaa tatcaagacc gtcgaattcg atctttcctt tattcagttc
    18601 ataaaaacgc atcagcagat tgactaaagt cgtttttcct gcaccagttg gaccaacgat
    18661 cgctaccgtt tgttttggct ttactgaaaa atcgacttga ttcatcaata ttttatctga
    18721 tgtgtaacca aactgtacat ttttgaaagc tatttttcct ttcggttgtt ctaaaagtct
    18781 tgcatctgga aggtctggaa tttcatctgt ttcatctaat atctcaaata tcctatcgat
    18841 tgctgccatt gcggattgga tcgagttgat cacataagaa gcggtagaaa ttggttcaga
    18901 aatctgattg atgtactgaa gataagcttg taagaagccg atcgtcacac cacctgatag
    18961 aacaagcata gcaccgaata ccgcacttat gatgaaggcg agttgattaa tgaaacgaat
    19021 ggccggatag atagcaaagt tcataaattg agcacgttta aaagcgttgt attgcttttt
    19081 atttacttcg tgtatagcag cttctgcatg agcttgctga ttgaatgttt tgaccactag
    19141 attaccagct aagatttctt ccattcgatt atttaaaaca cccagttcat actgattctg
    19201 ttcagaaatc tgtttgtttt tgttcgcaat cttagttgta acaattgaac tgccaacgat
    19261 cagcaataag acgagcaaag taagcttgct ttcgatataa aacagcatca gaccagcagc
    19321 agctattgtt acgacagaag taaaaaattg attgatacct gtcaataaca cttgagaaag
    19381 ttgattgaga ccggtagttg tccgactaat gatgtcacct acttgatgat tgtcaaaaaa
    19441 cgacatcggt aatgttttga atttttttgt gatttctttt cgcaccctca atgtgatctt
    19501 ttcgcttaac gctgccattg tccgttcctg aataaatgaa gtgatgctgc ttaagactga
    19561 acagccgatc agcaataaga cagggaacaa caatgcttgt ttgatatcac tgaaagaaag
    19621 ctgtccaata cctgtttttc ttatttctga taagagatca tcgatcccga ttcccatgat
    19681 caagggcatg gctacgacta ataggttccc gaataaactg catagaaaca atccgtaaaa
    19741 atatttatgt tcaggtttga tcattttcca aaaacgtatc aaagcacgcg tgttcaacgt
    19801 ttttttcttt tccatcgtat cccttctttc tatattcctt gtgattttgc aaactcctga
    19861 taagtttggc aattagacag tagttcttca tgagtacctt gtccaacgat ttttccttca
    19921 tctaaaacaa tgatgttatc tgcttgttgg attgtactga ggcgttgagc aacaatcacg
    19981 agagttttct ccgacatgtg tttatgaagg gctgctctta atgctgcatc tgttttatag
    20041 tctaaagcgg agaaactatc atcgaataca tagatctctg ctggtttgat caacgctcga
    20101 gcaatacata tccgctgttt ctggccccct gaataattag aaccgccttg agcaacaaag
    20161 ctgtccaatc cgtcaggaag ggtgtccact agttcttcta attgagcgat cttgatcgct
    20221 ttagacaact cttctgttga ggcatattca tttcccatct gcaagttgct taaaattgtc
    20281 ccgctgaata agaaagcctt ctgagggaca taactgatgt gggagcggat ctgttcttga
    20341 gtcatttgag taagaggaat accaccaagg gttacttttc ctatcgtagg gtcactaagt
    20401 ctcaagagaa gtttcgccaa actacttttt ccagatccgg tcccgccaac gatcgctgtt
    20461 gtttttcctt taggtattgt gaaatcaata tcttctagaa ctggatcaga gatttcagta
    20521 tatttaaatg tgacatgttc aaaagcagct gcatttgtct gatcaaaggt tggttcagct
    20581 actgttttga tcgtctcttc ctcaatttcg atctctgctt gcagtatttc ttctatccgt
    20641 cgtaatgagg caaacgattt agggataatg accaatacca tagctgcgat catcaagtaa
    20701 gcaagtgtca gcatagagta ttcgactaca gcagtcacga caccgatttg taatgctcct
    20761 tctgctacaa gatttccgcc aaaccacaaa atagaagcat aaacgattcc catgagcaaa
    20821 aaagcaattg gagtgaccca agcaaatatt cggttcactt tgatcatttg attagcatag
    20881 tcagcaaagc tcttattcgt tcgcttttct tcatatgatt gattatcaaa ggcacggatc
    20941 atattaatcc cagtaaagaa ttctctcaat gtgatggtga tccgatccat ttttggctgg
    21001 atactcaaag ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca tacataaaaa
    21061 acaacagaga tcaatgtaac ccatgttagt aatagagaat aacgaaacat catgagaatg
    21121 gtgaaaacgc aaataaccgg tgctggtaag atcatttggc aaaataaaac aatcatttgc
    21181 tggacattat cgacatcatt cgtcattctt gtcaaaagag atccagaact aaaacgactg
    21241 atatccttca ttgacagctg ttggaattta cggaaatatt tctcccgcgt ttggaatcca
    21301 aacctagcgg aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat tgttcctaga
    21361 aacgctacaa gcagcatttc aactccaatt ttttttataa ctgaggcttc gccacttgct
    21421 atacctgtgt cgatcagctt tgctactaat tttggtacac ctaatgtacc ggcgatttgt
    21481 aaacagatca tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa gaatgttttt
    21541 aagatagaca tattattcct ccttttttcc tgatactttt cgattctata ccttcaagta
    21601 acttgaaggt caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa
    21661 tgtatacgat tggccaagta tcggaactat ttgatttgcc aaaatcaacg atccgctatt
    21721 gggatgaaca aggattaatc cgttcaagta gacaagaaga gaatgattat cgattgtttg
    21781 atatcgatga tatttttatg ctttatgata tcgtttttta tagaaaatta gatataccga
    21841 tcaaacagat gaagaattta tatggaaaaa cattgacgga gttatatgag acacttgatg
    21901 aaacagaaag aagaatccat aaagaactgg tggtgatgaa acaaaaacaa aaagagataa
    21961 gagagcaaaa aaaacagttg aaattgatga tcgatacaaa cgaggaagaa tttcctgtag
    22021 aagagatccc ttttgactgt atgatctcaa cggagtttga agatattgtg gaaatcaaaa
    22081 aatttctacc taactattca agctttggga tgatgagtat gccagcttcc agttcacaga
    22141 cgatgtatgg attttttatc gaccctagtg aagtacattt atttactcaa gacgtgattt
    22201 gggagaaaaa agatacggct gtttatcgtc gattcttatt gaaatccgag atgaaccatg
    22261 cggaacgtaa taatatttta gaaatcagag aaagaatgta tgaaaaagga tggaaaacag
    22321 gagaagtgat cggccagtat ctattgacga atacggatga aaacaatatc agaactgaat
    22381 attatcatgc atggatagaa atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa
    22441 tgatggttgt tttagttata aagaaaaatt ttctggttcg attacgggtt taatgtgggg
    22501 gattattagt cgtggataga tagaatttta gttttttaga ggaaatatgt ctttaaaaat
    22561 ggaatggttg caattcttag cgatatatac gatgtaattg tcctcttaaa aaagtatgga
    22621 aaaata
//
LOCUS       DAJSMI010000029        21928 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJSMI010000029 DAJSMI010000000
VERSION     DAJSMI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.029"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            296..1198
                     /locus_tag="ON430_000919"
     CDS             296..1198
                     /locus_tag="ON430_000919"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR3218850.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            1411..3186
                     /locus_tag="ON430_000920"
     CDS             1411..3186
                     /locus_tag="ON430_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCR3218851.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3252..4838)
                     /locus_tag="ON430_000921"
     CDS             complement(3252..4838)
                     /locus_tag="ON430_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR3218852.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            5089..5313
                     /locus_tag="ON430_000922"
     CDS             5089..5313
                     /locus_tag="ON430_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCR3218853.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            5455..7206
                     /locus_tag="ON430_000923"
     CDS             5455..7206
                     /locus_tag="ON430_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3218854.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            7206..8990
                     /locus_tag="ON430_000924"
     CDS             7206..8990
                     /locus_tag="ON430_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218855.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            9316..10167
                     /locus_tag="ON430_000925"
     CDS             9316..10167
                     /locus_tag="ON430_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3218856.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            10221..11972
                     /locus_tag="ON430_000926"
     CDS             10221..11972
                     /locus_tag="ON430_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3218857.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            11976..12146
                     /locus_tag="ON430_000927"
     CDS             11976..12146
                     /locus_tag="ON430_000927"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3218858.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(12186..13088)
                     /locus_tag="ON430_000928"
     CDS             complement(12186..13088)
                     /locus_tag="ON430_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3218859.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            13319..14722
                     /locus_tag="ON430_000929"
     CDS             13319..14722
                     /locus_tag="ON430_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3218860.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALNDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            14722..16155
                     /locus_tag="ON430_000930"
     CDS             14722..16155
                     /locus_tag="ON430_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3218861.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            16300..17226
                     /gene="trxB"
                     /locus_tag="ON430_000931"
     CDS             16300..17226
                     /gene="trxB"
                     /locus_tag="ON430_000931"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR3218862.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            17440..19167
                     /locus_tag="ON430_000932"
     CDS             17440..19167
                     /locus_tag="ON430_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCR3218863.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            19182..19532
                     /locus_tag="ON430_000933"
     CDS             19182..19532
                     /locus_tag="ON430_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR3218864.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(19624..20484)
                     /locus_tag="ON430_000934"
     CDS             complement(19624..20484)
                     /locus_tag="ON430_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCR3218865.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            20576..21004
                     /locus_tag="ON430_000935"
     CDS             20576..21004
                     /locus_tag="ON430_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3218866.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            21166..21342
                     /locus_tag="ON430_000936"
     CDS             21166..21342
                     /locus_tag="ON430_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR3218867.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            21369..21815
                     /locus_tag="ON430_000937"
     CDS             21369..21815
                     /locus_tag="ON430_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCR3218868.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7139 a         3818 c         4681 g         6290 t
ORIGIN      
        1 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
       61 aataatagaa caaaaaaaca gacaaggctg tttttggcag gaatttcttt tttaaaagag
      121 ttaaaacgtg agaaaatgat ctttattata aaagagttct taagcttttc tttagagaaa
      181 atttcagtta ctatggagaa cagggcaaag aaaaatcaat cattttcttt gtatatcatc
      241 ttaccaatat ataggaattg ttagtaacaa ttgcctttca aggaggaatc catccatgtt
      301 attaggctca cacgtcagca tgagcggaaa gaaaatgctg ttaggagcag cagaagaagc
      361 agccagttat ggatcttcga cttttatgat ctatacaggc gcccctcaaa acactagaag
      421 aaaagccatc gaagagatga atattgatgc cggaaaagcg tacatggcag aaaaaggtct
      481 aacaaatatc gtcgttcatg ccccttatat cattaatttg gggaatacga tcaaaccaga
      541 aatgtttcct tttgccgtcc aatttctgag agaagagatt gttcgtgcgg aagctttagg
      601 cgctactcaa attacacttc acccaggtgc tcatgtaggg gcaggaacag aagccggaat
      661 tgctagaatt attgaaggct tgaatgaagt tttgacaaaa gaacagacgg cacaaatcgc
      721 tttggagaca atggcaggta aaggtacgga aatcggtcga acatttgagg aattagcgca
      781 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgaca cctgtcatac
      841 gaatgatgcc ggttataata ttaaagaaga cttttcaggt gtaatggaag agtttgataa
      901 gatcatcggg ttagatcggc ttaaagtact ccatatcaat gattctaaaa atccgcaagg
      961 ttcgcataaa gaccggcacg ccaatattgg ttttggaaca atcggattcg acacgctgaa
     1021 taagatcgtc catgatccaa tgtttgacaa tatttcgaaa atcttggaaa ctccctatgt
     1081 aggcaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatcgcca tgttcaaagc
     1141 gcaaacattc gatccagaag tgtttcctga gttattagca gaagaagtga tactctaaga
     1201 ttttctatct gacagcagca aaaaaggacc aggcacattg ccttgtcctt ttttgtttaa
     1261 gaaacaaagg agtacttttt gcagtctttt tcatcatgca tcttctcccc cctcttttta
     1321 aatttagaca aacaaggtat actggtagaa aagtaaaaaa acgaaaagtt tcacaagcaa
     1381 gtggcaaaac gtgattggaa ggacgaaaag atgctgaaca ctttaaaacc cttgatcaac
     1441 caactaaaaa agaaacatac atactttatt cttggcatag ccctattatt tacaattatt
     1501 cgtttgtatt tatatagtaa agcaatctat ggaacagaca tgtcggcaac ctatgatgac
     1561 cagctgctga ttcgctatgc tcaaaatatt ttggacggta aatggctagg ggagtatacg
     1621 actacaacac tttccaaagg aatctcctac agcttatttt tagtacttgc acataaactg
     1681 catctttctt atgcagtttt gttcggcatg ttcagtattt ttgccagtat attggttgca
     1741 ctcgcgttta aaccgattgt gaagaacaag tatcttcttt taagtatcta tttttatttt
     1801 ttgttttctc cgattagttt tacacatgaa tattccactc gaacatatcg taatgccctc
     1861 gtgattccgg ctgttgagat cattatcgct tgtttattag ctatttatta ccgcaaatac
     1921 ggttctagca gaaaattgct gccatggttt ttagggcttt ctgtcgcttt tcctttcttt
     1981 tggttcattc gcgaagattc tatctggctt cttcctttta ctgtagtagc tttgggtatc
     2041 agtttcgtcc agatcttgct agggcaagac ttttctttca aagaagtaaa gaagttaaaa
     2101 aaacgactct ttcttctctc gaaacgggaa tggctgaaac tttcattgtt tttacttcct
     2161 atagttggct tttccatcgt ctatcaatgg atcgaatata aaaatgagac aacatacggt
     2221 attgcaacca tcaatgaccg ttccagtgga gaatttggac gtttgatgaa acaactgatc
     2281 cgaatagatg atgaaacgaa cttgaataaa gaaaacagtg agatatgggt ctcacatgct
     2341 gcactaaata aagcattagc cgcatctcct acattccata ctgtttctac ccgtattgac
     2401 gaattatata cgactcacgc gtggactcaa ggtggaaaag taaaagaatt acccggagat
     2461 atcatctttt gggcattgcg agatgtggta agcgaatctg gttattacca aaacaatgca
     2521 aaagaaacca atgatttttg gaaaaaagtc aaccaagaat tagaagaagc ctatcacaag
     2581 ggaacaatca aaaagaaaaa agagttctat ctgatgggat caggagatgg aaagacgatg
     2641 agtgatctgc caatcgtatt cgatttcctt caatcaggat acgcttatgc atggcgatat
     2701 aaagatttcc atcaaggagg aaattatagt ttcggaacgc aagaacaagt caaacaggct
     2761 caagaattat taaaccttcc gctaatggag aattggatcg atagtaatga accgagaaca
     2821 cctaatttcc aattaacaaa atcagcaaaa gtcgcgaatg tcatcataaa aatttaccaa
     2881 gttctggcta ttcccgtgct gattgcttca atagcaggga tattacttat gatactagga
     2941 accgtatttg caaaagctga tcgtgcctat tatagttcct ctttgattgt ggtcaccgga
     3001 ttaatactga cacaattggt ttttcttttc ggtgtctcat ggttttgttc ttatgcgcca
     3061 gaaaaaaagg actatttttt aagtgtctac actggtgcag gcgtgccatt aacgcaattc
     3121 atcgtagtaa caagcatgct agttttcagt aaattaaatc tgaaaaaagc tttatcttct
     3181 aaataaaaag acccttggta gctagatttt taacctaact atcaagggtc tttgtgtgga
     3241 aaaatcagtt tttattcgat ttcttctacc gtttcctgca aaagctgttc atattttttg
     3301 tttcctttat ataaatcacc aatcgtccat gccaacagga gaaggacaag aacaatgatt
     3361 acataagcga tgtgatcagc caatgctagt tgagcggaag tggcttgctc gccgaagaaa
     3421 ctttcgatct gtcctggtaa tcctcgtaag ttcaagtaag ttagactaat cacagagaac
     3481 cagccaagaa gttttacgat gaatgaattt ttaaatcggc tacccatctc cgcttggctg
     3541 ttggtcatca tcagtaaagg aatcatcgaa aacggcaacg caaaagccaa gaacacctgg
     3601 gattcattca ttaagttatt caaagcggca tgttcttcga tcgttccttt tgtacttgta
     3661 aacaaaacac aaatcaatac aggaatcaca gaaagcaatc gtgtgaccag tcttcttagc
     3721 caaatcggga tacgcatatg gatgaatcct tccatgatga cttgaccggt caacgtacca
     3781 gtaatagtag agttctggcc agaagcaaga agtgctacag caaataaagt ggacaagata
     3841 ccagattttg ctacagcaat caaaatacca ttgctcatag ctgatggatc agataaagct
     3901 tcatacagac caaaaaagga aggatcttgg acagaaccgg ttttgaagac agcgacaccc
     3961 atgatcaata gcagtgcatt gactagaaaa gccattgaca actggatatt agaatcccaa
     4021 gtagaaaaac gtacagcttg tgctacatct tcttcgtttt ggtgatcgat cttccgagtt
     4081 tgtgaaactg ctgaatgaag atataagtta tgcggcatga ctgttgcacc aataatccct
     4141 aaagctccag tcagcggagt ttcgccgccg atgaccggtg agtctgagaa tgtatgactg
     4201 ttagggataa agccagcaaa taaactgtgc caatctgggt tagacaagcc aacttgatag
     4261 acaaaaacga ataaaatgac gaaaattaag cagacaacca gtgcttcgat cttacgaaac
     4321 ccgacttttg tcagtagtaa gagaacgaaa acatctaata cagtaataaa gacggcaatc
     4381 accaatggaa tatcaaaaag taagtagagg gcgattgctg caccaatgac ttctgcaata
     4441 tctgttgcca taatggctaa ttcagtcaag atccataaaa tgacacctaa tgtcttactt
     4501 gtgcgtgctc ggattgcctg tgccaaatcc atttggctca cgattcctaa ttttgctgcc
     4561 atatactgta aaagcatagc aatcagacta gacatcaaaa taactgacat caataaatat
     4621 tggaaatttt gtcctccggt gatcgaagta gaccagttac ccgggtccat atacccaact
     4681 gctactaacg caccaggtcc agagtaagca agaagtgttt taaaaaaact tttccccttt
     4741 ggtacatcaa ctgttccatt tacttcagct aaagataaac catttgtatg ttcaataagt
     4801 cgatgcttat gaaattgttt attggattga ttattcaaga aaaaaccacc tttcctattt
     4861 atctacgatt acattctaca cctcaaaaag aaaaaattaa agaaaaagat tcggtacgcc
     4921 taattttttt tcatggcgcg tcttgctaga tgacgaaaga aattttgttc tcctctaaaa
     4981 aaaccactat tttttgcatg aaactaaaga atacgccttt cttttttcaa taaactttag
     5041 taaaataagt cattgagagc aattcggaaa tagaaaggag agatttatat gttatcaaca
     5101 ggtattgtcg tacttattgc tattattgct ttgctaatcg gcgcagtagg tggctttttc
     5161 ctagcgcgta aatatatgaa agactattta gaaaaaaatc ctcccgttaa tgaggacatg
     5221 ttgcgatcaa tgatgatgtc aatggggcaa aaaccatctg agaagaaaat tcgccagatg
     5281 atgcagcaaa tgaagaacca agggaaaaaa taggttctga cccaacgact ttttacgggc
     5341 tgattttttc agtccgtttt ttatttcgta atttgttaga ctattcagat tattcagcaa
     5401 aaaagacaag cacagacaat cctaggctct tgataaagac aaaggaggag taaaatgtcg
     5461 atttttagaa aattaggctg gttttttcgt caggaaaaga aacattattt aattggggtt
     5521 ttctcgctat tcgcagtggc cttggtacag ttagttcctc caaaagtgat tgggatcatc
     5581 attgatgaga tcgccgataa aaatttatca ataaagatca ttttcgggtg gatcttccta
     5641 ttgattatcg ctgctttttt acaatatctt tttcgttata tctggcggac gaatatttgg
     5701 ggaagtgcag ctcgattgga gaaagaactt cggcagcagt tattcaaaca ttttacagaa
     5761 atggacaatc tcttttacca aaaatatcgg actggggatt tgatggctca cgcaacgaat
     5821 gatttgaatg cgatccagaa tgtagcagga gctggtatct tgacctttgc agattcagtg
     5881 attacgggcg gaacaacgat cattgcgatg attttgttta ttgattggcg cttaacactg
     5941 attgcgctga ttcctctgcc tttgctggcc gtcacttcac gagtgctggg gtcaaaactc
     6001 catgatgctt ttcgtgattc gcaagaagct ttttctagta tcaatgataa gacacaagaa
     6061 agtatcactg ggatcaaagt cattaaaaca tttggacaag aaaaagaaga tctagcggat
     6121 tttgaagaaa agattgaaga tgcaattatt aaaaacaaac gagtcaattt tttagactcc
     6181 ttatttgatc cattcattac cttgattatc ggtctgtctt atgttgcaac aattatttta
     6241 ggtggaaagt atgtcttgga aggcacgatc tcgatcgggc agatggtctc atttattagt
     6301 tatatcggta tgcttgtttg gccgatgttt gcgattggtc gattgttcaa cgtattggaa
     6361 agaggaaatg cttcttatga tcgcgtcaat gaacttttgc atgaaaagac gcatatcgtg
     6421 gaacaactgg gtgcagtgca gaccccggca aaaggcgagc tgaatgtagc tatttcctct
     6481 tttaattatc cgaaagaaga atcaccagca ttagaacaaa tcaagtttgc gatccaagaa
     6541 ggagaaacat taggtattgt tgggaaaact ggtgcgggaa aaacgacgat catcaaatta
     6601 ctgatgcgtg aatacgatga ttatcaaggg aaaatccaat ttggcggacg ttcgatcaaa
     6661 gaatattcat tgaatgcttt attaggatca atcggctatg tgcctcagga tcacttcttg
     6721 ttttcgatga ccattcgaga taatatccgt ttttctaatc caaaactttc agaagaagaa
     6781 gtagagcatg cggcttcttt agcatttatc aacaatgaaa tcaaggaatt accaaaaggt
     6841 tatgatacct tagttggaga acgtggagtc tctttatctg gcggacagaa acaaaggatt
     6901 tcgattgctc gagcattgat caaaaatcct gaattattaa tattagatga tgcactctct
     6961 gcagtagatg ccaaaacaga agaagctatc ttgcataatt taaaagaaat aagaaaagag
     7021 aaaacaacta tcattgcagc tcaccgttta agtagcgtga tgcatgcaaa agaaatcatt
     7081 gttgtcgatc aagggaaaat cattgaacga ggaacccatc atgagctgct tgcactaggc
     7141 ggctggtatg aacaaatgtg ggagaaacag caattagaag cgaagataga aggaggcgag
     7201 gcctgatgga tgaaaattat caatcggaat ggacaaaatc gattccactt aaagaacaga
     7261 aaaagatcat caaacgactg atgaagttcg ccaaaccttt tagaagaacg ttcatcgttg
     7321 ctatcttatt tgcgtttgct ttgtctgtga tcaatgtcct attgccaagg atcatccaaa
     7381 cttttatgga tgatcatctg gctaaacagt cggctacgac acaagttatt ttgttttttg
     7441 ctggtatgta cttatttgga gtcatagtaa aaagtatcat ttggtttttc caatggtatt
     7501 tgtattccat ggcctcattg aagacctatc agtatattag ggtaaaatta tttgaaaagc
     7561 tgcatacact aggtatgcgt tattttgatc agacaccggc aggatcaacc gtttctcgag
     7621 tgacgaatga cacagaaacg ctatttgaat tttggtacgt ctttttgatg gtattgactg
     7681 ggatctttgc agtcgtttct tcattccttg ccatgtttca aatcaatgga gaaattgcct
     7741 tgtggacttt ggtgtttttg ccgattttag gaatcgtcat ctggtattac cagaaattca
     7801 gttctcgtat ttaccgaaat atgcgggaaa aattaagtca gctgaacacg aaattgaatg
     7861 aatcgatttc cggcatgcgt attatccagc agtttcgcca agaagctcgt ttagctaaag
     7921 aatttgaaca agtgaacaat gagtatttag acacgcgtta tgcgatgatc aaaacgaatt
     7981 cgattctgtt aagtccgatc atcaattttc tatatgcatt ggcgattgcg ttggtgttga
     8041 ctttgtttgg gatcgatgcg ttgcattctc ccgtagaagt tggactgatc tatgcattta
     8101 cgacctatgt ccagggattt tttaatccaa tgtctcaaat gatggacttt ttgagcatct
     8161 tcacagatgg gatggttgct ggtagtcgta tcttgaagat tatggatacg gaagaatatg
     8221 caccacaaca aaaccctgga gcagatcaaa tgattacttc aggtaagatt gagtttcgca
     8281 atgtaagctt ttcttatgat ggagagcaca atgtattaaa caatatctct tttgttgcta
     8341 atccgggaga gaccgttgca ttagtgggac acaccggaag cggaaaaagc tcgatcatca
     8401 acgtattgat gcggttttac gaattttatg aaggtcagat attgattgat gatcatgaca
     8461 ttcgagaata tccaatggaa gagttacgta agaaaatggg gctggtcttg caagatgctt
     8521 tcttgtttta tggggatatc gcaggaaata ttcggatgat gaatccagct atcacagacg
     8581 aacagatacg ccatgcggct gagtttgttc aagcaaacaa attcatcgat gaattaccaa
     8641 aaggatatca ctcaaaagtg atcgaacatg gtgccagcta ttctagtgga caacgacagc
     8701 tgatcacttt tgcgcgaaca atcgtgactg atcctaaaat tctagtattg gatgaagcaa
     8761 cagcaaatat cgatactgaa acagaaggat tgatccaaga aggattggca aaaatgcgtc
     8821 aaggacggac aacgattgcg attgcccatc ggctgtcaac gattcgagat gcagaattga
     8881 ttttagtttt agacaaaggt catatagtag aacgcgggaa tcatgaaaca cttctagcgc
     8941 aaaatggttt gtatgcagat atgtacaaac tgcaaagcag tggtatataa aagatgttgt
     9001 gacagtagaa ttttgaccta agcactagag tgaaaataaa acatagcttc tcatattttt
     9061 catctgtgag agttagtgtc gtagtcacca gatttaaaaa aagattcgac agctagtcag
     9121 aagtgcctaa tagccactaa gaaagcgttg acaaaagcat ttgctttcga taatataaag
     9181 aaaacgatac aacttggata gtgattgcct gatgcaagct aaacctgatg agtctcccat
     9241 ggtttcatgg acggcttttt gggtttttct ttttgttttg gatacagccc tatacatcat
     9301 ggaaaggagc aaaacatgct gattgagaaa gtactgaata acaacgtcgt aataaccagg
     9361 gatgaaagaa atcaggaaat gatcgtgatg ggaaaaggtt tggcattcaa aaagaaagtg
     9421 ggtgacccga tttttgactc atgcatcgac aaaaaatttc agttgacaaa tgacgggctt
     9481 tcacaacagt tccaagaatt ggcacaagcg attcctcttg agtacttaga aatttcttgt
     9541 gaaatcattg aatatacaaa agaggctcta caagtccagt tgaatgattc tgtgtatatt
     9601 gcattgacgg atcatttgta tacggctatc gaacgtcaca aaatgggaat atttgtacca
     9661 aatgtattgc tgtatgatat caaacaacta tttcctaaag agtatgccat cgggaaaaaa
     9721 acagtgaaga aaatctcgga taaataccac atcaagttat cagaagatga agccggattt
     9781 atcgcattgc atttagtcaa tgcacaaggc attcaaagcc agcaagacaa tacctacctg
     9841 tttactataa caatacagga tattctaaat atcgtccgct attactataa gattacattt
     9901 gatgaagatt cagtccattt ttttcgattc gtcacacact tacgtttttt tctgacacgc
     9961 gtcctagatg gagaaaaatc agaagtggga gaagtttctg aggaattgtt tcaggttatt
    10021 caaaaaaaat ataaggatac gtttggttgc gttgagaaaa tagcagactt tttagccgag
    10081 acgttcaact accagatgtc cacggacgaa aaaatgtatt taacgatcca tactgcgcgt
    10141 ttagtgcatg agaatcgttg taaataaaaa aaggaggata aattaacaag aacgtatgaa
    10201 ataagggtag tgacattcag ttggctaaac cttccactaa taaattggag ggaatagaaa
    10261 tgggaaaata ccaagaatta gcgagaaaaa tcgtagaaaa tgtcggcggg aaggaaaata
    10321 tcaatggttt gacacattgt atcacaaggc ttcgtttcaa attaaaaaac gaagaaaaag
    10381 caaatgatga gatcttgaaa aatatggacg gtattgtaac ggtaatgcga gcgggagggc
    10441 agtatcaagt tgtcattggg aatcatgtgc cagtcgtgtt tgaagaggtt ataaaagcag
    10501 gaaaccttac ttttgatgag gctgtttcaa cagaaaaaat gagaccgttt gatatgctta
    10561 ttgatggaat atcgggctgt ttccagccat tccttgctat cctagctgct ggcgggatga
    10621 ttcgtggact gacagcattc cttgtcttct taggcgcgtt tgaccgcggt tctggcacat
    10681 ttgttatgtt tgataatatc ggtgattctg tattccaatt tatgcctgtt attatcggtt
    10741 taacagctgc ccgtaaattt aaagtaaatg aattcgtagg gatgttgctt ggcgcagcat
    10801 taatgaatcc tagcttgtcg ctggaagctt tgagtggtgc agcagaagca ccactaacga
    10861 ccatattttc tggcactatt tttgaagcac ctatttatca aacagtattc ggcattccct
    10921 ggattgcgcg taactacgct tcttctgtca ttcccattat ctttattgtg ttgttagcta
    10981 gccaaattca gaaaccaatt aaaaagttag tacctgagat gatcgcgaat ttcttcgttc
    11041 cattcttcac cgttcttatt acaatgcctt taggtttttt actcgttgga ccagtattca
    11101 cattcgcaac tgatatttta atggctggat ttgaaacact tttagcactt tcgccagtta
    11161 tttatggagc aatcgtaggc ttcttctggc aaattctggt gatgtttggt ctccattggg
    11221 caatcgtacc aatgggattg atgcaatttt ctgtgaatgg ttggcaaaat attatgacac
    11281 ctgttgcagt agtgtctttt ggacaaactg cagcacttac cgcactctac tttaagctta
    11341 gaaatccaaa agacaaagca attgcaatac cagccattgt ttctggtatt gtggggatta
    11401 cagagccagc tatttatggg tttacattac cacgtaaaaa aatatttatt tttacctgtg
    11461 tcggtggtgc gttagggggc gcgtatagtg ggttaatgaa tttaacttct tggaatcaag
    11521 gtggactagg catctttaca attcccaact atattagacc agatggtgat ttaacagatg
    11581 tgattaatgt attgattggg atcgccattg cgatggtggt atcctttacg ctgacttttt
    11641 tcttttggaa ggatgaagca ggtgaaacag atgatataca gaaaaaatca ggtaaagaaa
    11701 ttgtaaaaac accgatacaa ggacaaatcg ctccactaaa tgcagcaaag gatgcagctt
    11761 ttgcacaagg aacactaggt cgaggaatct tgatctaccc tgaaaaagga gaagtacgtg
    11821 ccccttttga cggaacagtc atgaccctgt tcccgacgaa acaggcaatt ggaatggttt
    11881 ctgaaacagg gctggaattg ctgattcatg tcggattgga tactgtgcag ttggaaggga
    11941 aatattttga aagcttagtg cagcaaggat aaagagtgaa acaaggcgat ctgttgctac
    12001 gcttcgacaa aaagaaaatc gaaaaggaag gatacagttt agaaacacct gtgatcgtca
    12061 ccaattacac ggattatttg gatattttgg aaaaccaagg ggagacggtc gggccagacg
    12121 atgcacttat caccgcactg acctgaacgg ttaggtgaag aatggactag gcttaactat
    12181 tctttttatt ccacttcttt attttcaaaa ttgaaaggct ctttcatgat ggctgagctg
    12241 atagggcgtt tatcgaattt ttgaccgaca gaaattagat ggactaaatt ctgttgataa
    12301 ttttctgcaa aaacacaact atataacacg tctagaagat aacaaattga tgtattgatc
    12361 gtaaaattac caatttttga atacagtcgc tcgcgtgtcg taagtcgcaa agcggctgta
    12421 ctcattgcag atagcgttga atcaccgata ctggtcaaag ctaaaattgg aacatgattt
    12481 tgtttcaata tttctgcggt acgaaggatc acattgtttt cacccgtata agaaatcaga
    12541 atcccgcaca tatcactcgt gcaattgtac gcttcataga atgattcacc aaaagtggtg
    12601 acaatcgtga catccatttt gatgcgcttc attttcagca tgaaatcttg agaaatcaaa
    12661 gtattcgagt tactggcgaa aatcacgatt ttttttgctt gaagcattag ttgtttggct
    12721 ttttgcagtt ggtcatgatg aatgagagaa agtgtatcat gaatcgtcat ctgttctaaa
    12781 gaagccattt tatttgcgat cgtcataata ccgtctgtgg tggaaaaagg aagattcgca
    12841 tctacgtctt gaaaattccc ttcaagatag gctgtttcag ctaaaaaagc attacgaaaa
    12901 ctgacccagc catcaaaacc catttttttt gctacacgga tgagtgtaga cgggtggaca
    12961 tagcatcgct cagcgatctc ttggatggtc atttcgccaa gtgctggaga atcgcttaag
    13021 agaaactgga tcacttcatt ttctgaagga gaaaaggaga tttctttgat tttttcacgt
    13081 aataacatgt agttaccttc ttcttatttt tttacgtaaa ttataacttt tttgaaaatg
    13141 ttttgtaaac ggatttatac tatataactt aaaatagact ttttttgaca agatatttac
    13201 aaaaatccac tagaaaacag taacttttgt aaatgttact ggaaagaaac ggatttctat
    13261 tgtttgtttt gtttacaaaa aagataatga aagtgtaaaa aagtaaagga gtgagaacat
    13321 ggcaaaagga atcaaaatcg ctacgattgg tggaggcagc agttacaccc ctgagttgat
    13381 ggaaggattt atcaaaagat atgaggaatt accgatacga gaaatctggc tcgtagatgt
    13441 agaagaagga aaagaaaagc ttgagatcgt tagtcagatg tctcagcgta tgtgggatgc
    13501 ttctccgtat gaagtcaaaa tctatgcaac tctggatcgg gaagaagcat tgaatgatgc
    13561 ggattttgtt acgactcagt ttcgtgtcgg tcaattagat gcacgaatca aagacgagcg
    13621 gatccctagt tattatggga tgattgggca agaaacaaat ggtgctggcg ggatattcaa
    13681 agcttttcgt accgttccag tggtcttggc aatcgttgaa gacatgaagc gtttatgtcc
    13741 aaatgcttgg ctgatcaatt tcgcgaaccc aagtgggatg gtgacagaag cagttatcag
    13801 atatggcaaa tgggataaag tgattggatt atgcaatgtg ccagtaatgg cgatgatgac
    13861 agaaccagca atgcttggca agaaaaaaga agagctgatc tacaagtttg ccggtctaaa
    13921 tcatttccat tggcacaaag tagcagataa taaaggaaat gaccgcacgg cagaattgat
    13981 tgatcgttta tacgatgaag acaatggatt gccgaaaaat atctttgata tcccgttttt
    14041 caaagaacaa ttaaaacaaa tgaatatgat cccttgtggc taccaccgct attactaccg
    14101 tgaagaagaa atgctggaac atgctttaga agaatatcaa acgattggta caagagcgca
    14161 gcaagtgaaa gaaacagaag cagagctatt tgaattatac aaagatccag aattagacca
    14221 taaacctgaa caattgcaaa aacgaggtgg cgcttattat tcagacgctg cctgtgaaac
    14281 gattgcttca atctatggaa ataaagaaac acagatcgtg gtatctacaa agaatgatgg
    14341 agctgttcct gacctgccgg cagattgtgt agtagaagtc actgcctatg ttggcgcaca
    14401 aggagcgaga aatgtagcgt ttggttctct tcctgctgct gaaagaggct ggttgcaagt
    14461 aatgaagaat atggaattgc tgacgatcga agcagctgtg actggtgatt atgggacagc
    14521 tttacaggcg tttacgatca atccgcttat acgatcagga aagacagcaa aacggatcat
    14581 ggatgagttg tttattgcac accagcagta tttaccgcaa ttcaaggaaa caatcagccg
    14641 attaaaagaa gaaggaatcg aagttcaaga tgaactggca agagaattat caggtaatac
    14701 acatataatg gaggaagtat aatgggattc agaaaagatt ttttatgggg cggtgctaca
    14761 gcagccaatc aatgtgaggg cggttatgat gaaggtggac gcggactagc aaacgtagat
    14821 gttgtgccaa tcggaaaaga ccgttttcct gttatcaccg gaaagaaaaa aatgtttgaa
    14881 tttgatgagg atcattttta tccagcaaaa attgcaatcg atatgtatca tcgttataaa
    14941 gaagatattg ctttgtttgc agaaatgggg ttcaagactt atcgaatgtc catcgcctgg
    15001 agtcgtattt ttccaatggg ggatgaaaaa gagccgaatg aagctggctt gaaattttat
    15061 gaggatgtct ttaaagaatg ccaaaagcac ggaatcgagc cacttgtgac gattacgcat
    15121 tttgattgtc caatgcattt gatcaaagaa tacggtgcgt ggcgttccag agaactcgtt
    15181 ggtttttatg aaaatctgtg tcgagtcatc ttcaatcgat ataaaggact tgtcaaatac
    15241 tggctgacat tcaatgagat caatatgatc cttcatgcac cattcatggg agcaggactg
    15301 tactttgaag aaggagaaaa cgaagaacaa gtcaaatatc aagcggcaca tcatgagttg
    15361 gttgcaagtg ccatcgcaac gaagattgct catgaagtag atccggagaa caaagtaggc
    15421 tgtatgctgg cagcaggagc atattatcca tacacttgca atcctgaaga tgtttgggct
    15481 tctagaaaat cagatcgaga aaattatttc ttcattgatg ttcagtcacg aggagaatac
    15541 ccagcttatg cattgaaaga aatggaacga aatggcattc agatcgaaat ggaaccaggg
    15601 gatgccgcat tgctgaaaga acatactgtt gattttatct ctttttctta ttattcttcc
    15661 cgagtttcga ctaccgacaa cgaactcttg gaacagactg caggaaatat ctttgcttct
    15721 gtcaaaaatc catatctgca agcaagtgaa tgggggtggc aaattgatcc attaggcttg
    15781 agaataacga tgaatgatat ttatgatcgt tatcaaaaac cattatttat tgtagaaaac
    15841 ggcttaggtg cagtcgatac gccagatgag aatggttatg tggaagatga ttaccgaatc
    15901 gattacctgg cagcgcatat ccaggcgatg aaggatgcag tagaactaga cggagtcgat
    15961 ctattaggct atacgacttg gggatgtatt gatttagtat cagctggtac cggtgagatg
    16021 aagaaaagat acggattcat ttatgtcgat cgtgataacc aaggaaatgg cacattagcc
    16081 cgttcaaaga aaaaatcatt caactggtat aaaaaagtaa ttgattcaaa tggagaaaat
    16141 ctttcgaacg attgatcaat gatttataaa agtctattgt ttttcagcgg atttttcgag
    16201 tataataaag ctattcgaga aacgcaagca gaacaataga ctttttttcg cattggctgt
    16261 aaagtatgct tacttgatta agtaaaggag aatctacata tgtatgatgt aatcgttatt
    16321 ggcgcaggtc ctgctgggat gactgctgct ttatatgctt cacgttcaaa cttatccgta
    16381 ttgatgatcg aacaaggtgc ccctggcggc caaatgaaca atacggcaga aatcgaaaat
    16441 tatcctggat tcgattcgat catgggcccc gatctagctt ataagatgta tgaaagtgtc
    16501 tcaagatttg ggacagaaaa tgcttatgga atcgttcaag atatcaagga tcacggcagc
    16561 cataaagaag tcatctgtga agataaaagt tacgaaggaa aaacagtcat tatcgcaaca
    16621 ggctgtatcc accgtaaatt aggcgtacct ggcgaggaag aatttgctgg acgaggcgtt
    16681 tcttactgtg cagtctgtga tggtgcgttt ttccgtaaca aacgtctagt agtcatcggg
    16741 ggtggggact cagcagtaga agaagccatc tatttgacac aatttgcttc tgaagtagtg
    16801 atcgtccacc gtagagatga attacgtgca caaaagatca tccaagaccg tgcatttgca
    16861 aatgaaaaaa tttcctttat gtgggataca gtcgttgacg aaatcgttgg aaacgaaatg
    16921 gtagtgacag gtgtgaaagc acgtaatgtc aaaaccggca atgaatttga tttagcagca
    16981 gacggagtat tcatctatgt tggactggat ccattaactg caccttttag agaagcaggt
    17041 ttgacgaacg aagctggctg gattccaaca gacaacgaaa tgaaaacgaa tatgccaggc
    17101 gtttttgcca tcggagatgt tcgtgaaaaa gatttgcgac aaatcactac agctgttggt
    17161 gacggcggta ttgctggaca acaagtgtat aaatacttgg aatctttaga agaaacaact
    17221 gcataagttt aaaaagagta gaagaacggg ttcaattgtt cttttactct tttttttcgc
    17281 gtgaaattta ctgaatctga tttatgcgaa cacgtccaac accttttgat aaaagaatcc
    17341 tttcttgatt taagcgtttt ttggtcctca tactttcata tctaataagt gaatgctata
    17401 cttattaaga accatatttc agaaagaggt gttattttca tgtcttggga acaagtgtat
    17461 gaacaatggg tacatgaaga aaatttagaa gaaaacttaa aaaaacaatt aaatgatctg
    17521 ggtgaagatc ctgaaaaact tgaagatgct ttttatgcac cgttggaatt cggtactgca
    17581 ggcatgcggg gaatcttagg acctgggatc aatcgaatga acatctatac ggtaagacaa
    17641 gcaacagaag gactagcacg tttcatggat acacaaggtg aagaagcaaa acgccgaggt
    17701 gtcgctattg cttatgactc acgccatcag tcaccagaat ttgcaatgga agcagccaaa
    17761 acactggcac atcacaatat cccgtcttat gtattcgaaa gtctgcgtcc aacaccagag
    17821 ctttcttttg cagttcgtca tttgaagacg tttgcgggaa tcatggtaac tgcatctcac
    17881 aacccagcgg cttacaatgg atataaagtc tacggagaag atggcggaca aatgcctcct
    17941 gcagacgcag atgcattgac gaaatatgtc cgtgaagtca gcaatccgct aaaagtcgaa
    18001 gtattatctg acgaagaagc taaacatagc gggttgatca cgatcatcgg agaagaagtg
    18061 gacgctgctt atctagaaga aatcaaagca gtgacgattg atcgtgaatt ggtagaaaca
    18121 atggggaaag atttgaaact tgtgtacacg ccacttcatg gaacagggaa aatgttgggc
    18181 gaacgtgcgt tgaaacaagc tggattcgaa caattcgtac tagttcccga acaagctgtg
    18241 gcagatccag atttcactac ggtcaaatcg ccaaatccag aagaacattc agcttttgaa
    18301 tatgctatac gacttggaga aaaagaagga gctgacttgc tgattgcaac agatcctgat
    18361 gcagaccgtt taggcgcagc ggttcgtttg ccagatggtt cttatgaagt attgactggg
    18421 aaccaaatcg gggcattgtt tgctcaatat attttggaag cacatcaatc aagaggcact
    18481 ttaccagaaa acgcagcggt attgaaatca atcgtttcca gcgaattgcc tacagcaatt
    18541 gcggaacact acggtgctac catgttcaat gtgttgacag ggtttaaatt cattgctgaa
    18601 aaaatccaac aattcgaaga agaccattca catactttta tgtttggatt cgaagaaagt
    18661 tatggttatt tagttaaacc ttttgttcgc gataaagacg cgatccaagc attgctttta
    18721 ttagcagaag tagcagctta ctataaaaaa caaggcaaaa cgttgtatga cggcttgcaa
    18781 acgatttttg aaacctatgg ctattttgca gaaaaaacga tttctgtgac aatgagcgga
    18841 caagaaggtt ctgcgaaaat ctctgctttg atggaaaaat tccgtaaaga agcaccgaca
    18901 gaatttgccg gagcaaaagt tattcaaaca gaagacttta aagaattgac gagaacagac
    18961 cgtgaaggaa acgtagaaaa actaacaaca ccgccatccg acgtattgaa atatacattg
    19021 gaagaaaatg gctggattgc tatccgccct tcaggaactg agccgaaaat caagttttat
    19081 attggtgtaa aaggaagctc tacagaagac gcagccgaaa aagtagcctc ttatgaagcg
    19141 attatcaaaa agatgacaga agaataaaaa acttgagact gatggaaaaa gtggtaaact
    19201 ttagaagtca gtcttttttc aagttggagg tgaatggatt gccagacacg caaaaatcaa
    19261 aagtagagaa agtcagtcaa ttattcaaaa tgttgagtga tcccactcgt cttaaaattc
    19321 tgctatatct gaaagacggg gaacaaaatg tgacagcaat cacccaagca gtggaaatgg
    19381 agcagtctgc tgtatcgcat caactacgtt tattaagaga aaatcatgtc gtcaagtctc
    19441 atcgcgaggg aaaagcgatt ttgtatagtc ttgatgattc tcatgtactg gatattttga
    19501 accagacact caaacatgtg gaacatcaat aacgataggt gcaaatagat aaacactgga
    19561 gtgtgacaac gaaatttctt cgttgtcaca ctccagtgtt ttttaagaaa aagacaaaaa
    19621 tgattaatcg cttttttctg ttgtttcagt tccataataa ctatgatctt ctaggtctaa
    19681 agcattcaaa ataagcgaag ccgtttcttc aatagataaa gatgccacat tgatcactac
    19741 acatcctagt ttttcataaa gatcattcgc aaaatcgagt tcagctctaa ttttttcgat
    19801 atcggaataa gcagtatcag ggtttaatcc gtaagagcgc atccgttctt tacggatatt
    19861 atttaaaata tctgggttat tcgttagtcc cacgattttt ttaggatcga tctcccaaag
    19921 ctgtttggga atatgtgctt gtgggatcaa aggtaaattg gcaactttta aatttttgtt
    19981 agctaaaaac aagctcaaag gcgttttgga agttcgggat acgcctagca aaacgacatc
    20041 tgcttctaaa aaaccacgag gatctttccc atcatcgtat tttacagcaa attccattgc
    20101 tttgatccgt ttgaaatagt tctcattcaa taaatggagg gcaccaggtt gccgaattgg
    20161 cttgataccg gttaaacgtt caatttctga tatcggtggc gtcatcacat caaaactaaa
    20221 taattgatgt tcttcacaaa aagaacgtgt tatctcgatc agttcttgat tgattaatgt
    20281 atgcaggaca agtgcttttt cttctaatgc atcttgcaat gcctgcttta atgttttttc
    20341 atctgtcacg aacgttctgc ggaataggtt gaactcaacg gtaggatact gagccatagt
    20401 agcttgcgct agttttgtcg cagtttctcc agcagagtcg gagatgacaa aaaaagtaac
    20461 gatttctgta gaatcggaag acatggataa aacctcactt gcattttttt tatgtttatt
    20521 ataccatact ctagaatgcg tacttcgtaa tcattttgat ttggaggaat aagtcatgaa
    20581 aaaacagaca ttgatcgaac aatcattaga aacattgaaa aataacggtt ttaaatatac
    20641 taaaaaaaga gaagcattgt taacttattt agtgaagcgt aaccgctacg tatctgctaa
    20701 agaagttttt gactatatga atcaggaatt caaaggggta agttatgata cgatttatcg
    20761 gaatctccat gattttgcag agatcgattt actggaagaa accgatttaa atggagaaat
    20821 gaagtttcgt tttcattgct gtcaggggga aatcggccat caccatcatt ttatctgtac
    20881 agtgtgcgga acaacgaaag aaatccatat gtgtccgatg aattatttcg aagaacagct
    20941 aggtggatgc acgatcgaag ggcatagatt cgagattttc ggaagatgtg aaaattgtca
    21001 ataagggttt aaataaaata acatttgata attttaaaaa aaagcaagat ttccttgacg
    21061 gtcaaaacag cattagatat aatatttaca ggacagtctt gttttttgag atatatttca
    21121 ataacaatta cggttacttt ttaagctcgg agggagggaa ttaacatgtc aaaaacagtc
    21181 gttcgcaaaa acgaatcact tgacgatgct cttcgtcgct tcaaacgttc cgtgtcaaaa
    21241 gcgggtactt tacaagaatc tcgtaaacgt gaattctacg aaaaaccaag tgtaagacgc
    21301 aagaaaaaat ctgaagcagc tagaaaacgc aaaaaattct agttctgtga tgaattggag
    21361 ttggtagtgt gtcactacta agtagattaa acgatgacat gaaagcagcg atgaaagcga
    21421 aagataagga atctttacaa gtcatccgaa tgatcaagtc atctatacaa aacgagcaga
    21481 ttaaagaagg tcacgacttg acagaagaag aagaactaac ggtgttgtct cgcgaaatga
    21541 aacagcgacg tgattctttg catgagtttg aagaagcagg acgtgacgat ttagctgaaa
    21601 aagtaaaaag tgaaattgta atagtcgaaa aatatatgcc ggaacaactt tccgatgaag
    21661 aaattcgaca acttgttcaa gaagcaatca ctcaaactgg tgcttcatca atgaaagaat
    21721 ttggcaaagt tatgggtgca attatgccta aagtcaaagg aaaagcagat ggaaaccaag
    21781 tgaatgcgat tgtgaaagaa ctattacaat cttaatttta tcatttaaac tgcggacagt
    21841 gattgtccgc agttttttgt gttcgtaaaa aaagaactaa aaaacagcta gaagaaagaa
    21901 tcttattctt tcttctagct gttttttt
//
LOCUS       DAJSMI010000030        21380 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJSMI010000030 DAJSMI010000000
VERSION     DAJSMI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.030"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(390..1781)
                     /locus_tag="ON430_000938"
     CDS             complement(390..1781)
                     /locus_tag="ON430_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCR3218869.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1994..2380)
                     /locus_tag="ON430_000939"
     CDS             complement(1994..2380)
                     /locus_tag="ON430_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCR3218870.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2436..3059)
                     /locus_tag="ON430_000940"
     CDS             complement(2436..3059)
                     /locus_tag="ON430_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCR3218871.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3123..3791)
                     /locus_tag="ON430_000941"
     CDS             complement(3123..3791)
                     /locus_tag="ON430_000941"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR3218872.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3803..4243)
                     /locus_tag="ON430_000942"
     CDS             complement(3803..4243)
                     /locus_tag="ON430_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218873.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4302..4973)
                     /locus_tag="ON430_000943"
                     /pseudo
     CDS             complement(4302..4973)
                     /locus_tag="ON430_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5158..5556)
                     /locus_tag="ON430_000944"
     CDS             complement(5158..5556)
                     /locus_tag="ON430_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR3218874.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5553..6326)
                     /locus_tag="ON430_000945"
     CDS             complement(5553..6326)
                     /locus_tag="ON430_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR3218875.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6323..7006)
                     /locus_tag="ON430_000946"
     CDS             complement(6323..7006)
                     /locus_tag="ON430_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR3218876.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7173..8060)
                     /locus_tag="ON430_000947"
     CDS             complement(7173..8060)
                     /locus_tag="ON430_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3218877.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8057..8926)
                     /locus_tag="ON430_000948"
     CDS             complement(8057..8926)
                     /locus_tag="ON430_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3218878.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9063..10349)
                     /locus_tag="ON430_000949"
     CDS             complement(9063..10349)
                     /locus_tag="ON430_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3218879.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10622..11200
                     /locus_tag="ON430_000950"
     CDS             10622..11200
                     /locus_tag="ON430_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3218880.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11200..12129
                     /locus_tag="ON430_000951"
     CDS             11200..12129
                     /locus_tag="ON430_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3218881.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12126..13862
                     /locus_tag="ON430_000952"
     CDS             12126..13862
                     /locus_tag="ON430_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR3218882.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13925..14761)
                     /gene="pheA"
                     /locus_tag="ON430_000953"
     CDS             complement(13925..14761)
                     /gene="pheA"
                     /locus_tag="ON430_000953"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCR3218883.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14758..15267)
                     /locus_tag="ON430_000954"
     CDS             complement(14758..15267)
                     /locus_tag="ON430_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCR3218884.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15260..16555)
                     /gene="aroA"
                     /locus_tag="ON430_000955"
     CDS             complement(15260..16555)
                     /gene="aroA"
                     /locus_tag="ON430_000955"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR3218885.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTNTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16568..17659)
                     /locus_tag="ON430_000956"
     CDS             complement(16568..17659)
                     /locus_tag="ON430_000956"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCR3218886.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17676..18842)
                     /gene="aroC"
                     /locus_tag="ON430_000957"
     CDS             complement(17676..18842)
                     /gene="aroC"
                     /locus_tag="ON430_000957"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR3218887.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18844..19908)
                     /gene="aroB"
                     /locus_tag="ON430_000958"
     CDS             complement(18844..19908)
                     /gene="aroB"
                     /locus_tag="ON430_000958"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR3218888.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19941..20963)
                     /gene="aroF"
                     /locus_tag="ON430_000959"
     CDS             complement(19941..20963)
                     /gene="aroF"
                     /locus_tag="ON430_000959"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCR3218889.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         6176 a         4688 c         3738 g         6778 t
ORIGIN      
        1 acaagtcaca acctctggta aacggtgttc tcctgacaac tcctcggtca caagacacaa
       61 tattcaacaa acatgagaag ctattttttg aaattgctct ttgtgactca gagtgaaacg
      121 gcaggctcac gacctctttc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagct
      181 caaccaaacg taaaatatga ggaactattt ttgtttggct gttcttatta cttgatgcag
      241 ggcagctttt tcacaacctc ttattcacgg tgtttcagaa gtagcttctc ggaaataagc
      301 cgaaatttca caaaaatttg agaaactatt tttaggaaac ttcttcttat ttcttaaagc
      361 tgaccacttc tatcacaacc tctttttgac tatgcattca catcgatcac tttagaatca
      421 tcattgatga catcgcgatc taaatctttg atgcgtgcac ttgtcacgat accagctacc
      481 atgctaccgc tgacgtttac tgctgtacgt gccatatcga ttaatggttc aacagagata
      541 actaacccaa cgattgctac tggcaagttc attgccccta aaacgatcag tgaagcaaat
      601 gttgcgcccc caccgactcc agctacacca aaagaactga ttgttacaat tgccacaagc
      661 atcaaaataa attccaaact aaagacattc actccagctg ctggtgcgac gatcgttgcc
      721 aacatagctg gataaacgcc agcacagccg ttttgtccaa tagacaaacc aaaacttgct
      781 gagaaattag ctgttgcatc atctactcct aaagcttttg tctgagtctc gatattcaat
      841 ggcaaagctc cagcacttga acgtgaagta aacgcaaagc tcaataccgg aaaggctttt
      901 ttgaaatagt tgattggatt gacttttgca ccaatcaaaa tcagtgagtg gacaatcaaa
      961 acagcaagca atgctacgta agaagcaacg acaaatttac ctaaattgac gatagctgca
     1021 aaatcacttg ttgccaaaac gtttgtcatc aaagcaaata ctccataagg tgtcaaacgc
     1081 aaaacaagcg tcacgatccg cataatgatt ttatacagac tgtcgactaa atctgaaaag
     1141 aatgctgctt cttttggcgc ttttcttttc acgcctagat aagcgattcc gacaaatgcc
     1201 gcgaaaataa cgactccaat cgtacttgtt gcacgactac ctgctaaatc agcaaaaacg
     1261 tttttaggga taaaggataa aatctgctgt ggaatcgata agttttcaac tgtttcctga
     1321 cgtgttgcca gttcagagat tcgtgctgtt tctgcagctc cttgtgtgaa tttagctcct
     1381 tggagtccga acagcattgt tgaaccaatt ccgatcaatg ctgcaatagc tgttgttcca
     1441 agtaaagaag tcaatacagt cgcactaatt ttccctagct tttcagaaac tttcattttt
     1501 gtgaatgcac caatgatcga aataaacact aaaggcataa ctagcatttg caagaatgat
     1561 acatatcctt gacctacaat cccgatccat tccattgatt gagtaacgac tttgtcgtct
     1621 acaccaaaga tcaattgaag cagaccgcca aagacgatac caactcctaa tgctgtaaag
     1681 acgcgtgttg aaaattttgt atggcgcttt tgcatacgat agaacacata taataatgcc
     1741 gcaaaaacta gaaccactaa aactgtgatg agtgttgtca tttttctcct cctcatatta
     1801 ttaatgacaa gtccatcgaa ggaagtaaag gttcccgaga atgattcttc aaccatcctc
     1861 ctgaaaaaat ttttttcagt gacttttata ttttagacga tttttatcga aatgacattc
     1921 tttttgctta caattagtaa taaaaaaaca tacctgtgat agctttaatc tatcgcaggt
     1981 atgtttttac tgtctattct tgtttttctg attgaaaatt caattttacc ccaggctgat
     2041 ctaaggcgat ctctgtcacc tcataagaca ttcggcgtac gatatcgaat aacggtgcta
     2101 ctagctcgtc cacttcttct tgtgtgacat cattttgacc ttcaattttc cgattgagga
     2161 tatgattgat ctgactcaca cttccagata aaacatatgt atcaaacact aaacggaact
     2221 ctaagcgtgc tcctacgatg ctatgttctt tcggatagtt ttcgatcgtt tcttttaaag
     2281 gcgagaagcc tactttcaaa tctgtttcta cttcttggtt cggcaatctc atatcataat
     2341 gaaatgcttc tacaacttct ttttgtcttc tgatttccat ataattttat cactccttta
     2401 tgaaaattat aacatatctc ctttgaaagg ggatactact ttttaatgat gctctgcaag
     2461 gtaaggattt cattcagatt tcctaccaca taatctgccg ggatctcacc cagaaaatga
     2521 tcgatgtcgt aaagacaagt agcgactcct gcatttttcc ctgcttcgat atctaaacga
     2581 cgatcgccga tcatcatcgt gtctgttctt tctaaatgaa aagtatcgat caaataattt
     2641 aaagatgctg gatcaggttt acgaggaaag tcctgatcga tccctactac ttcctcaatc
     2701 agatgtgcca atcgatgttc ttttaacagg ccccatgttg attctgtcaa acggtgggtc
     2761 aggatgaaat gacgaccgcc gttgtctttt agcatttcca gtacttgttt cgtttctgga
     2821 aatggacggg aaacttccgt actttctttt tcatatcggt gaaataattc attaaactct
     2881 ctggcattca agtttgattc attgatcaaa taactagtag aatacttttt cattttgaag
     2941 tagatttctt ttttgtccat actgatacca aaatctttta gtgcttgcca tgcaccatct
     3001 accattgcgg gatacgtatc aaacaatgta ccatcaaagt cccatatata atttttcatg
     3061 ttccttttgt tccattcctt tgcttctttt ttctatctct cacttttaga taaaggtgtt
     3121 ctttaaagta tttttttgtc cagaattgtc aaatcatctg ctaatgtata ctggattggt
     3181 tgtgcatttg ggatatcgat cgtatccatt tgatcttcag gaacatcttc taaatatttt
     3241 gtcagtgctc ggatgctatt accatgaccc acaactagta aatcttgccg atcttttagt
     3301 tttggtgcta gatgatcttg ccataaggga atgatccgat tcactgtcat ttccagactt
     3361 tctccgtatg ggatcaaatg cggatccaag tgatcatatc tgcggtcaaa tctattttct
     3421 gcggttaacg gcggaacttc cgtatatcct ctgcgccatg tttttacttg atcttttccg
     3481 tatatttctt tcatcttatc tttgttttta cccacaagat ccccataatg ccgttcattt
     3541 agacgccatg tttttattat aggaagccat aactgaccca tttcttctag tgcgacttgt
     3601 ccggtaagaa tcgcacgttt caaaacagag gtataaacgg catcgaattg aagtccggct
     3661 tcaagcattt tttgaccggc aactgctgct tgttcttttc ctttttctgt taaggcgaca
     3721 tctagccatc ctgtccagta attttcaaaa tttgcttcgc tttcgccatg acgtaataat
     3781 actaatttca ttcttttctc ccttaaacat tcgtttcgaa ttttatacca catgttttgc
     3841 atgtcacacg tattcgtcct tttcctttag gtgcacgctg cttttgatga caattcgggc
     3901 actcaaaaaa agtataactt tttcgatcca ttttgacttg ctttttgttt tttatctgat
     3961 gatatttttc tttgactcta tcaagatatt tcaaataacg ttgattctca tttagtctgg
     4021 ggtagatttt ttttgatagg aaacgaaata tcatccatcc gaaaaagacg atgaacaaca
     4081 attgtccgat tctatagggc aaccaacgac ttagcaaact aaagacaagc gcagcgatca
     4141 atagaccgcg attcaactga tcaaatttcg catatcttcc ctgcataagt ttgtgatagt
     4201 tctgctgaaa tcgtattagc ctttctaacc atgtttgcat catattatct cctccatcat
     4261 ctaatattta ttatgacaaa aaaatccaaa aagaaaaagc cttaattggc cttttccatt
     4321 gcaacttggt gaaggacatt ccgatttctg gtaaagtcaa tcacaatcac tttcttttta
     4381 tcttctctgt tgactactcg aaatgtgtcc gtcaatggaa gaaacagctt cttagatggt
     4441 agctgttgca tacgtataaa acgatggatc atcgtaatca attgaggaaa ttgaatagac
     4501 gtgtgaagca gacctggata ttttttcaac agctgtgtcg ttttgatcag attccgtttt
     4561 ttactgatca cttttaaaaa gctttttaga atcttttgct gacgtctaaa aatagaaaaa
     4621 gaattttcgt catctatttg ataacgcatt tgtttttgca gttctgctgc agtcagatgg
     4681 atcttcccat cgtttgtatc gatcattaaa ccttctggaa acagttcaac cactgctcga
     4741 tcccaagatt caagagaaag ctcgatataa tcttttactg gtaattccaa ttctacctaa
     4801 aaaaagtgca aaacagccga cagcccttct gcttgataga tttctcctaa tgttagcccc
     4861 tctagatgga actctggtga aaaatgcagc aatgttttat tttcctgcaa ttgaacatat
     4921 agaaatgtag atcgctcaat cagagtatct tttaaaacaa ctaaaaaatt catttttctc
     4981 cccctttttt catcattttt aaaatagcat attttgaaaa gaaagtctga tgcttagagt
     5041 agaatttcat aaaaatttac tatttcataa gtattcacga gaaaaataca aacaaaaaag
     5101 cggaaataac gctcatccgc cttcttgttt gtgtttattt cctcttcaaa cgcttgtcta
     5161 tgattcagac ttttctctat cgttattttc atctaaaagt tcttgcattc cgataatttc
     5221 tcgatattga tgaatcttag tgataccaat gactttgtct acatcaatac taaaacaaat
     5281 accattacct ggaatatcga tgttgatgcc ttgaccaatc gcatccatta ctttattcgt
     5341 caaactatcg ggtactaatg tcaacacaat atctttttct ggcgaaatgt ccaaacctaa
     5401 aaatttccct gaatcatgta caccagaacc tcttccatgt aagatcgttc cgcctgctgc
     5461 acctgcttct tttgaaaagt cgataacatc ttcgcccatt cctccgtcaa cgatcgtcac
     5521 aatcatgtga agattcaatg ctacaagatc tttcatttgc tttcccccaa tttttgcaat
     5581 ctttcttttt cgattctgtc tttttcatct tttcgtttat aaatatatcc tagtactaaa
     5641 gtggataata ttggtgtcaa tgcaatcatt gcaaccacac caaacccgtc aatgatcgga
     5701 tctcttcctt caatttgtgc agcgattgcc acgctcattg ataaaataaa tgtcgaacac
     5761 atcgggcccg ttgctactcc accgttatca aaagccattg ctaaaaagat cttgtccact
     5821 tttcgtccta aaataaatgc tagcccgtat cctgggatca agaaataata aagagagaat
     5881 ccgacgagca aacgccacat agacaatcct actgctgcgc cgatcccaat tgaaattacc
     5941 atcagcatga ttttcgatcg gattctcccc tcgctcaatt cttcaacctg tttgatcatt
     6001 acatgaatcg ccggctcagc aaaccctact aagaacccca ttaaaaagcc aaggggaacc
     6061 agtatataat tgtaaggcaa tcccgcaata gcactgccta aatgctgtcc taccggtaca
     6121 taagccatat tgactccgtg caggaaaagg atcaacccaa tagtcgttat caagaaacct
     6181 ttcataatgt caaagaaacg ctttttgcgc atacgaaaac taatgatatt cattaaaaca
     6241 aataggatta cgataggtgc cactgccaaa atgacttcca tggtaacgat ttcgattcca
     6301 tctatcactt gctgccagaa actcattgga agatcacccc caaaatcatc acggcaagaa
     6361 tcggccctaa cgaagcaata ccgaccattc caaaactgtc attttcattt ttcttttgtt
     6421 tgatcatact agtcatccct ccggcaaggg ccaagataaa tggaacagtt actggaccag
     6481 tagtcactcc ccccgaatca aaagcaatcg gcataaattc ggaactggtg aaaaatgagg
     6541 cgatcaaaac gcctatgtag cctatagcca tcaattgata ataggatagc ttgaatacga
     6601 cccgaagcag cgcaaaggct agaaatactc cagtgccgac gctgacgatc ccaatcagca
     6661 acactctgcc aatctctccg tcagatatat catatacttg ttgtcccaaa acttgcacgg
     6721 aaggttcagc aatcgtgatc acgatcccga tagcaaatcc caaactgacc aaaacggtca
     6781 aacttttctt tttgatcata tatttcccta ctagatctcc gacttccatc atagaataat
     6841 ctgctccaaa caaaaagagc gtcattccga tcatcatgat tgccgcacca accagaaatg
     6901 aagcgaggtc ttctccttct aacggtgcaa aaacaaacgt taaaatgaca atcaggacag
     6961 tcatcggcaa tatagcagca atcacttctt taaaattttt tagcatgtat cctcctcctt
     7021 tactctttaa gtttacctta aaatgaaaga gttttcgagc acaaacttaa aacgttatca
     7081 tttaattttt ctcaacataa aataacacac gtcccaaaag tatcttttta cttggaaaaa
     7141 tgactttgaa ggaacgcgct tattgtgttt ttttatttta cagagccgat cattccttga
     7201 acgaattgct tttgcaaaac aaaaaatacg atggcagttg gtaaagtagc gatgactaat
     7261 ccagccatga tcattccata gtctggtgta taggaagcac ccatagcaga taaaacaagc
     7321 ggaaccgtcc gtttatcagg agattgcaaa gctactaagg gccataaata attgttccag
     7381 ctactcatga atgtgatgat tgcagccgcg gcataggtat ttttagccgt cggcatatac
     7441 actctaaaaa aaatacttag ttcttttaga ccgtctaatc gagcggcttc tactagatct
     7501 tttggaaaag atttaacatt ttgtcgaaag aagaagatca aaaaagcagt gctaacagat
     7561 ggcaggatga caaccaagaa actgttgatt cccaaaaccg ttccgttcaa ttgtgaaaac
     7621 attcgatata aagggatcat caatgccgca aaaggaacca tcatcgatag cagtagaata
     7681 ttgaaaatta ggtcttttcg tttactttga taaatctcaa atccatatcc tgccattgac
     7741 gaaatcagca atgcaaacac agtggtcacc aaagcgatca tcgctgaatt ccaaagggaa
     7801 cgagtaaaat tcagctcatt ggaaaacaaa ttttgaaaat tgatgatcaa gttactgcca
     7861 atctttattt ttcccgatgt gatatccacg ggtgtattcg tcattcccaa aaccatccag
     7921 acaaaaggga aaatggaaat aaatgatagg atgcttaatg ctgtatactt taatactgac
     7981 ccaattgtcg aacgaagttc taatgcttgt tttttgacgt tggggcgttc gattgtttct
     8041 ttttctacca tatttttcat cgactttctc ctcctacttt cgtttggatt atcgacaata
     8101 tcacaataaa ggaaacaata acaaaggaaa ctgctgccgc atatccaaaa ttaggcgtga
     8161 atttataact caaattatag atgtattgag atagggtggt cgttgcattt gctggtcccc
     8221 cgccagtaat attttgtact tcatcaaaaa gctgcaatgt cccgatcgtc gaagtaatgg
     8281 aagtaaacaa aatgattggt tttaaatttg ggatcgtcac tttgataaac tgctgccatt
     8341 tagaagcacc gtcaatagat gctgcttcat aaatctccga atcaatattt tgcatgcctg
     8401 aaaggaaaaa aatcatattg tatccagtcc aacgccaagt gatcgaaaga atgatcagaa
     8461 ctctcgccca aattggatga gtcaaccagc caatcggtgt gtcaatcata ccaatgccca
     8521 acaacaagtt gtttacaagg ccagattgag aaaatagact tttcatgatc agagaataac
     8581 tgaccatcga agtgatacat ggaagaaaga tagcggttcg aaacaaccct ttatatttca
     8641 actttttatc atttaaaagg ttggaaataa aaagtgctag aaacaacatg atcggtactt
     8701 gtacaagtaa atacaggaat gtattaaaaa aagctttttt aaaagttgca tctgtcaaca
     8761 tccgctgata attacctaac ccgttaaatg tcatattgac agggggacca ctttgaaaag
     8821 acgtgatcaa agccaaaacc atcggaataa aaaccatgat tgatatcaaa atgactggca
     8881 agattaaaaa agcatgcccg tttttgaata aatctttctg cttcattctt ttccctccaa
     8941 ttctatcatt gattatctac atgaaaagac aagaaagaca atatctgtaa ggcagacgtg
     9001 atcttttttg tcttttcgtt tgtctgatga aaaagcgaag aaattcactt ttttaccacc
     9061 gatcaattag ccaattgtgc ttctacttgt ttttgtgtat ctgctagtac cttgtctgtc
     9121 tcttctccat tgatgattcg atgaagagct tcagctacga ctgcttcaat tgcataggtc
     9181 tcatgtccgt aattcacttc tggtatttca gatgtccatg ttgaaaaatc gtcaaagatc
     9241 ttttgaccac tgtaaaattc agaagcttct tgataagctt cttgttcatt cgcgctaagc
     9301 atcgtagata ctagtccgat ttctttcgca agagtgccca tcagttcttc attcgaagca
     9361 aacgttgctt ctaggaaatc tttcgctgaa tcttctccag ctactccttt gatcacatac
     9421 cagccagcgc cgccaaggtt agaagcttgc gctttttgca gattttctgg aagtgcaggt
     9481 attggtgcaa tcttccattt tccagattgg ttttctgctc cttggatcgt tgaagaatac
     9541 caagcgccag ttgggctaga agctactgct cctgattgta tcgcagttac tccagcattc
     9601 cagtcagagg tttgttcgac tagatcttct ttcaaaagag tggcaaacaa ttcaagacca
     9661 tactttaaag atgcattgtc ttggatcgtg actgtctccc catcttcaga tgtataccat
     9721 tctcctgcct gttgcataat catccttact cgaccaaggt cagaaggatt gacttcagcg
     9781 attttctttc ccgtcttttc tttgacatct cgcgctactt gaatatactc gtcccacttc
     9841 agattctcca tatcctcttc tgtataacct gcttctgcca tcagatccgt acgatagaag
     9901 tttgctgtta cgcccgaatc gaaaggaaca ccgtatattt tatctcctac tttgtttacc
     9961 gcaaatttat acggtgcaaa atgatcttcc tttacaatag cagacaaatt agcgaatgca
    10021 tctggataag aagtcaaata gccttgtatc ctataatctt caatcaagac gatattgggc
    10081 aatccttctg tattcccgct cgctaaagcg gtgttcagtt tctgaacgat atcatcctga
    10141 gacatcgtaa caacttctat ctctgtttct tcgttctcat aaactttttt tgcttcattc
    10201 actgctttga tattgaatgt ctcatcccac gcccagacag tgactttttc ttctttgtct
    10261 gctgtactac tagcacttcc gcatcctgtt aagaaacctg atgctgccgc taaacaaacg
    10321 actccagcaa ttaactttct gatgttcatt tttatccgct ccttttccat ccattagttg
    10381 tttacgtttg ctacattcat cataccaaaa tttgaaaacg gtttctttat tattcagata
    10441 cttcaaaaat ttctcctaaa ataataaaaa aatacaaatc aactatgaca aaaggaataa
    10501 tcttagcatc tagaccaaag atcgaaatgg tgaggtttct ttaaaataaa aatatccttg
    10561 cattttctcc tagaaataag tatttttaaa ttacaagact attcttaagg cgggtaaaaa
    10621 aatgccaata cgcgtaattc ttatcgatga tgaacagacg attttagatg gaatgaaaca
    10681 gttgatcaat tggtctgagc acgggtttca attaataggg actttttcca gtccgctgaa
    10741 agcgcttgat ttttctttgg tacatgcaat cgatatcgtg gtcacagatg taatgatgcc
    10801 tgaattaaat ggaatcgaat tatcgcggct attaaaacag caacaacctg aaacacagat
    10861 ccttatcctc tctagctatg atgaatttga tatggttaaa gattctttta aagaaggcgt
    10921 cgctgattat cttttaaagc ctcgattgag tcctgatttt tttctaaatt ccttaactgc
    10981 cgtttcacaa aaaatcaaaa aaaagcccaa caaaagccca atcaccaatc gttatgagca
    11041 gatctgcgag aatctttcac agcagattgc tggacaaact cgtccattgc ttgaccctct
    11101 tctttttgaa cacaccttgt ttttctgtta tataccgaaa aacggccatt cagttcaaaa
    11161 caaggctcta aacaaaaaca gtcgatcacc cattcattga tggatagcga cgattcgttt
    11221 actatttatc ctgtttctac cttttcggaa gaatccggct atgtaatcaa tactgattcc
    11281 aaagatacac ttaggaagtt tgttcaaaag tttcgtcaag aagtttttcc aggtgatttt
    11341 tttgttgttt ctgattcttt tgcaatccaa gatatcaatg aaatattcca agagatcaga
    11401 aattgcacca aaggacaacg tttctatcat aagggaaaga accttgctta tcagcatgaa
    11461 cttcttccgt tattaaccaa ccacgagcag caagcaagta gttatttaac aaaaatcgtt
    11521 cataaagact acgctttagc tgtcaaagaa cttgagaatt tcttcacgac gatattggaa
    11581 cagcctattg atccagctta tttgaaacat gaggcaatca atcgtttcta tgcattactc
    11641 aacgctttgg ctgacgagca tgttgggaat gaagaacttt ctttgctcaa gatcgccctt
    11701 ccttctttat tagccgatgc tgagtatttc gaagattttt ctattcttgt caaagaaaca
    11761 cttgagaaac tcacccactt ttctgcaaat gcaaaaaaag acaatttcga tatcttgact
    11821 ttgatctacg agttcgtcca ggaaaattat gagaaagaaa tcaatttaca acttttatcc
    11881 gaaaaatatc atttttcata tagctatctc tcagccatct ttactgaaaa atttggtatc
    11941 aacttttcaa aatatctaaa aaaagtccgt atcagtaaag ccaagctctt tttgacggaa
    12001 acacggctta ctttgacgga aatctgtgag aaagtcggct ataccgaact gggttatttc
    12061 tccagagttt ttaaagaaga gacaggaatg acaccttctc aataccgtaa aggaactttt
    12121 gtaaaatgag aaaaaaaatc aaagagcatg aactctttgc caaacttctg ttcattttgt
    12181 tcatcggtct ttttctgcaa gccatcgtca tttctttgtt tatttatcat cgatcaaggg
    12241 atgcctatat ccagctgttc aatcaatcga atgatgtggt actaaaaaag attcaatctg
    12301 aatttgaaag tttgaatgat acaatcgaga atacattagc cgcttttgat tctaatccag
    12361 ctgtcaaaag ctatttttcc aatgatccag cccaacatat ggaacagctt cagcaactaa
    12421 gaacaatcca aaaaatgaat gactctcttt caaaaatcca tccaatgatc gattacgatg
    12481 tattgatctt tggagaaaat ggaagaactt ttgtcggaaa cgacatgctc actgccgtat
    12541 ctgccgattc tttttttcaa tctgcgattg ctcagagagt gaatgaacgt gctgctgata
    12601 cacaaatgct ttttgcacac cacggattga ctcttcgcga taagaaagca cccagcgtct
    12661 tctttgtcaa aaaactgaaa aatacattga atcacgttta cggatatgct gttttatcga
    12721 tctcatccaa gcaactagcg aatctttttc aatctgtagt aaatcctgag atatcgaaga
    12781 tctccttcat gaacgaagaa cagacgatca tcgcatcaaa tgaagaaaat acaattggga
    12841 gccgatcaat gattttcaaa aaactaacag taggtgaaac aacgatttca aatggacagc
    12901 agttgacccg tcttcctctg taccgccagg attgtacatt actcagtcaa gtcgatcttc
    12961 attctttatc taaccaaatg ggcgtgattt ttccgattat tttgtataat atcgtgacac
    13021 ttgcttttgc tggctcattg gtatttatgt atttggatcg acacacaaaa tcgatctacc
    13081 gcttgattga ttcgctaaaa cggctagagg aagatggctt aaaagctact gttcctgtac
    13141 aaggaagcca tgaagtccaa ttgctaggga atacgatcaa tcaattgcta agtaatgctc
    13201 agcatcatta tgaacagacg atccaagacg aaaagaagaa acgttcgctt gaaatccaaa
    13261 ctatgcaagc acagatacag ccccatttta tttataatac gctgactagc ttgaaatttt
    13321 tgatttggca gaaaaaaaca gatgaagcag ttcaaggaat cgacagtttc attactttac
    13381 taagaagtac gattgggaaa aaagaagaag tgattccagt caaggaagaa atcaaaagcg
    13441 tacaaagtta catcgatctt ttgagtatgc gatatggcga aagaatctcg gctaaaataa
    13501 tgattcctga tgaactgcag tcattatcta tgccgaatat gatccttcaa ccaattatcg
    13561 aaaacgccta tcttcatgct tttcacaata aaacgcaagg attcatcacg gtgtacggca
    13621 aaacaaaaaa tcagcttctt ttattcgaag tgattgataa cggcgatggc ttcgatactg
    13681 aaaataccaa acaaaaaaat gatcattttt caggaatcgg catcagccat gtagatgagc
    13741 ggatacgcct aatgtatggc gatgcatatg gcttaacgat ccaatcagtg attgggacag
    13801 gaacgactgt aacgatttgt ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat
    13861 aaagaatagc caagcgctga tttttttatt ttaagttaaa taaatcaatc agtattcttg
    13921 actttcatct acgcgtctct tctactgtca gcactggata acagcccaat atttgacttt
    13981 ctcctccgag catcttgatt tcttggagcg cattctctac taatgtcatt ggacggtcca
    14041 acaacaaatc gatcacgaaa aaatattctc ccaagctagt tttcaatggt cgggattcaa
    14101 tcttacttaa gttgatttcc cgccagccaa aagctgccag cattttatga agcatccctg
    14161 gccgattagc aggtagtgtc aagatgacac tcatttttac tggagctgac tgctggctgg
    14221 tcatctttcg gtcaccgacg atccaaaaac gtgtctggtt caactcatta tcttggatat
    14281 tctcagctaa gatctccaat ccaacatgct gtgctgtttc taatgaagca atcgctgctg
    14341 cgtcttcttt cggatgttct gctacataca ttgctgcagc agttgttgat tctgttgcta
    14401 ctaaaggaac attaggataa tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg
    14461 gatgtgaaag gatttttgta atctttgttg ctggattgcc taaaagctgc tgcttgatag
    14521 gaagaacgat ttcactttta acttcgactt ctggatgttt ggaaagaaga tcgattgtgt
    14581 gatgaacagc tccttccaat gaattctcta caggaacgac agccagatcg acttgtttgc
    14641 gaaacaaagc gcgcaaacaa acagggatgg aagcatacgc agtcaattgt tctgtaggaa
    14701 atagctgaca agccgcctga taagtgaatg aactttctgg ccctagatag ctgacgttca
    14761 tatgcttttg atcctttcta taatggtatc gatgatcttt tctggtgttt gattggtcgt
    14821 gttgattcga tgtgtcgcac tttcttcata ccaagaaagt cgttgtgcca ataatgcttt
    14881 tatttcttct cttgtttttc caagagctaa aggtcgaata gacgtttcat cttgttggat
    14941 acgatcaact agacagtccg cttctgcctc taagtaaaga acaatcggct gtgtttttaa
    15001 aaaacgccga ttttctgcac tttggacaat ccctccacca gtcgcgataa ttttgtcttc
    15061 atttgacagc tttcttaaaa tatctgactc cacttttcga aagctttttt caccgtaata
    15121 gtgaaaatat tcggcaatcg acaattggat atgttcctca atctttgtat ctaaatctac
    15181 atactctttt tgtaaacatt gagctaaccc tttaccgatt gttgtttttc cagcgcccat
    15241 gaaaccaatc agcaaaatgc tagccatgag cttctcctcc tgacacaagt tctgctaaat
    15301 cgtcaaaaaa tgccggataa gagattgaaa cagcttcagc tccttctagt tcgcaaggtc
    15361 catcagctat caaagcagct acttgcagca tcataccgat tcgatgatcg cctctgctgg
    15421 acacttttgc gccatgcaga ggtgtcggcc catggatgat cagaccgtct gctgtcgctt
    15481 ttatctttgc tcccattttc tgtaattctt ctgccaccgc atcaattctg ttggtttctt
    15541 ttacttttaa ttcttctgcg tctttgatga tcgtgattcc ttctgcttga gtagccacta
    15601 aagctagaat gggcaattca tcaatcagac gcggaatgat ttctccacca atcgttgctt
    15661 tcttcaaatg actcgtcttg acacttaaat cagccgattg gttctgttca ttgtgattcg
    15721 tctgggtaat cgctgcgccc atctcctcca acacatctaa gataccagtt cttgttggat
    15781 tgaccccgac attttttaac aacagttggc tttccggaac aagaagtcct gctgctagaa
    15841 agaaagcagc tgatgaaata tcacctggaa cagttatctg ttgaccagtt aatttttgcg
    15901 gtccggtcac tatgatcgtt ttatcttcca ctgtgatcct tccgccaaat tgacgaatca
    15961 tttcttccgt atggttacgg gaacgttctt tttcaacgat tacggatgtg ccttctgctt
    16021 gtaatgccgc aaacagcaaa gcagatttga cttgcgcact ggctacaggc atattgtatc
    16081 ggataggtga taaagagcgg gttcctgtca cagtgattgg tagatactgg tcattttctt
    16141 ctccttgcaa gtcagcgccc atctcgcgta aaggttccat cactcgcccc attggacgtt
    16201 tagacaacga agcatccccg aacagagtat tcgtaaaagg ctgtccagcc aaaataccca
    16261 tcaataaacg tatggtcgta ccggagtttc ccatgtcaat tgattcggtt ggttggacaa
    16321 atgagtggct accgcgacca tgaatcttga tcacctcttc ctcttcttcg atctcgacac
    16381 ctaattgttg gaaggcatgt aaagtactca gacaatcttc tgcgcgcaaa aaattttgta
    16441 tagtcgtcgt tccttcagca attgctccaa acatgatgct gcgatgggaa attgatttat
    16501 ctgctggtat cctaacagtc cctcttaatc catgtatttg ttgtaataat tgcatttatc
    16561 cagcctccta tttgatccag caatgataac tggtattaga cataatgatt ctttttgctt
    16621 gtaacaaatc ctgttggttt ttaaaactga tttgcagtat tcctaaaata tcttccctgg
    16681 tctcttggat cttcaaatta atcaaagaaa ctcccgcttt tccgatcagt ccagttactt
    16741 ctgcaataac gccaggttca tctggcacat cgataaataa atcaaaaaag gcaggaatcg
    16801 cgccttgctt atggacaggc aactggtctc gactttcttt tgcccgattg aaaaaagaaa
    16861 agatttccga ttggttctca gtcatgatcc attctgctat ttgtgtcatt tcttgttgcc
    16921 aatctgccaa tcgttccaat aatatctgct tattgctcaa caaaatatct gtccacatga
    16981 caggatctga ggaagcgatc ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg
    17041 ctcgcggata ctcttgattg aattgttctg cctgattgac taagccagct gccagaatat
    17101 gtggaaaatg acttagcatt cctgttatac ggtcatgttc tcttgctgta agagttacat
    17161 attttgcttt ggttccgcaa aatagttctt gcagtttttc aacatctttt ttcgtctttt
    17221 ccaaaggtgt aaagatgtaa taggcatttt caaatagttt agcatctgct gcctgtatac
    17281 ctgatttatg agaaccagcc attggatgtc caccgacaaa ccgaagattt ttctgctcag
    17341 cggatgtcac gacttgctgc ttcgtactgc tgacatccgt aatgatgacg tgatcagaca
    17401 agttgattct ttcaaggaca tcaagataag ctaaggttgt acggacggga acggctaata
    17461 agataacgtc tgcttgttct gcacctgttt caaaatcact tgcaacgtaa tcaacgatct
    17521 gctgttctac tgcttgctca cgggtttttg actgactatc ccaaccaact actatcagtt
    17581 ctggattggt ctgacgaata caaagtgcca tagaagcacc aatcagtcca agccccacga
    17641 taaaaacatg ttgtgccatc ttatttgccc cctttttagt aatgtttcaa gtagtcacgg
    17701 tactccttca ctgcagcttt caactcaaca agcgaatcac ttgggaattt tgacaatagc
    17761 tctgtcgcta ccacagtggc tacaacattt tccgcaacga cgctagctgc aggaactgct
    17821 gtactatccg aacgttctac acttgctttg taaggttctt ttgtatcgat atcaacactt
    17881 tgaagtggct tgtacaatgt aggaatgggt ttcatgaccc cgcgaatgac aagtggttcg
    17941 ccattggtca ttccgccttc gaagccgcct agattattgg tacgtcggaa atagcctctt
    18001 tcttcttccc ataggatctc atccatcact tctgagcctg gtaatgaacc agaagtgaat
    18061 ccgaccccaa attctacgcc tttaaaggca ttgatactaa ccacagcttg tgcgattttg
    18121 gcatctagct tcgtgtccca ttgtacgtag cttcctaatc cagctggaac gcctccaacg
    18181 agcacttcta caattcctcc gatcgtatct ccatttttct ttgtctgatc gatcaaatct
    18241 tttattcttt gctcaactgc tggatcaaca actcgtactt ctgacgcttc agaaatttct
    18301 ttgatttctt gaatcgagat atttccaggt atttctgctt ggattccacc aagacaaatc
    18361 acgtgtcctg ctacgatgat gtcaagttct tccagaattt tcttggcaat tgcgccgatt
    18421 gctacacgca tggtcgtctc tcttgcagag gaccgttcaa gcacattccg aagatctcga
    18481 tgatggtatt tcataccgcc taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc
    18541 cgcagtgatt ggtctttttc aggcacttct gctacagaca tgacagttgt ccaatttttc
    18601 caatctttat tttctaccac taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg
    18661 ccagacgtga tccgaacacg atctttttcg atcttcatcc ttcctcctct gccgtaacca
    18721 gcttgtctgc gagcgagttc gtgattgatt tcttcttctg acagaggtaa tcctgctggt
    18781 agtccttcaa taatggctgt taattctggt ccatgggatt ctcccgcagt catataacgc
    18841 atcttacagt cctcctaagt aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc
    18901 ttgcccaatc ttttctagca agatgatatt caattggctt cctcttgctt tcttatcatg
    18961 ggttatcgcg tggaataatt catctggatg acttagatcg tacgtaatcg gcagatggaa
    19021 tttttgaagt atcctttcta aagcagctgt cgatccagtt ggagacaagc cgattttttc
    19081 tgcatgagaa gtgatggcta ccattccaat agctactcct tctccatgac tgactacccc
    19141 atatcctgct gtattttcaa ttgcatggcc aattgtatga ccaaaattta aaataagtcg
    19201 atttccttgg tcaaagacat cttcttccac tacttttcgt ttgatttcca acgtagcagt
    19261 aatgattgga acagcatgtt ccagaagatc tttttcgtct ttcagctcgt tcagctgttt
    19321 ccaaagcgtt gcatctgcta tagctgctga tttgatgatt tcagcgattc cttctcgtaa
    19381 acgacgtacc ggcaaagttt ttagaacctc aggatcaatc aatacaccag ctggctggct
    19441 aaatgttccg acaaggtttt tcgctttgtt tgtattgact gctgtcttcc cgccaatact
    19501 agaatctact tgtgcaagta gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa
    19561 cgtcgaggcc acgaatgctg ctagatcacc caccattcct ccaccaagtg caagtatccc
    19621 atcgctgcgg gtaaaatcat tatctgctaa aaaatcgtat aactcagctg cttgttctaa
    19681 agatttactg gtctcacccg ccggtacgat tgaaaggact ggttcaaaat tagcttgtct
    19741 caattggaac atgatttttt ctgcatatag tggagcaacg tttgtatcag tgatcacagc
    19801 tactttttga ggtctccaaa tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt
    19861 ttcaatatga atatcgtatt tttggttatc aacatgtaca gtaagcattc gtttcacttc
    19921 tttcttttaa aaagctggaa ttaatcgagt gtctgaatct tttccattgc ttctttcatg
    19981 atcgcaactt ctttcatcat gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct
    20041 gaccaagcat ttgcaggatc aggatggatc tctacgatca tgccatctgc tccgcttgcc
    20101 acacccgcac gagccattgg tggtacaagt tcccaaattc ctgtaccatg acttggatca
    20161 acaatcacag ggaaatgagt taattttttc atcaatggga cagcacttaa atcaaacgta
    20221 ttacgtgtcg ctgtttcgta agtccgaatc ccacgttcga taaaaatgac aggagatttg
    20281 tcttcgactg caatgtattc tgcagcatta agccattcat tgatcgtacc ggaaattcca
    20341 cgtttcaatc caacaggttt acctgtttta ccgacagcag atagcagttt aaaattttgc
    20401 atattacgtg cgccaatctg taggatatcg ctgtactcag ccaccatatc gatatgccct
    20461 tcatccatca cttcagtaat cactttcatt ccaaattcat cagccgcttg acggatgtat
    20521 ttcaatcctt cttcttctaa accttggaaa gcatatggag aagtacgtgg tttgaaggca
    20581 ccacctcgca gaatttttgc gcccccggct ttggccattc gcgcacattc tctgatttgg
    20641 tctaatcctt cgatagaaca aggaccagcc atcgtcacga aactgccgtc accaattttt
    20701 actccgtcta catccacgac tgtattgcca ggatggaatt ctcgagaagt caatttgtat
    20761 gtgtttaaaa tacgcacagc attttctact ccttcatagc gtgtaaatgc tccttcttgt
    20821 aaacttcgcg tatcaccttt tagtccaatc actactcgtt ctgttccttg atcgattgag
    20881 actttgaatc cttctttttc gatatgatcg acgacttttt ttatttgttc ttccgttgct
    20941 tcttttttca taattactat cattgttttt cccgctttct tttgtttact tatctatctt
    21001 ggctgatttt ctctactttt tcaaggtggt tttacattaa ttcctactaa atgaccgtgt
    21061 cctgttgttc tcattttcgc tccttctttc ctacttatga cccagataat caacaaaaaa
    21121 agatgtccct gtaggaaatt gcctctacag tgaacatctt caacgagcct taaaaagcgc
    21181 tgcaaaaata taaaatagcc actatagatg aatttctcta cgtggctgta agtcgtgttt
    21241 aaaaatcttt agccatcata gatacaaacc tggtttgttt gtacccacga agtcatgaat
    21301 acaaacctat ctaaaatagc taaagtaata cgtattcagt tttgtaagtt ttctagattt
    21361 cataaaaaat cgctccaaac
//
LOCUS       DAJSMI010000031        21372 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJSMI010000031 DAJSMI010000000
VERSION     DAJSMI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.031"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(160..1281)
                     /locus_tag="ON430_000960"
     CDS             complement(160..1281)
                     /locus_tag="ON430_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3218890.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1407..2348
                     /locus_tag="ON430_000961"
     CDS             1407..2348
                     /locus_tag="ON430_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3218891.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(2498..3394)
                     /locus_tag="ON430_000962"
     CDS             complement(2498..3394)
                     /locus_tag="ON430_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR3218892.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            3663..5003
                     /locus_tag="ON430_000963"
     CDS             3663..5003
                     /locus_tag="ON430_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3218893.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            5221..5664
                     /locus_tag="ON430_000964"
     CDS             5221..5664
                     /locus_tag="ON430_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR3218894.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(6168..7586)
                     /gene="nhaC"
                     /locus_tag="ON430_000965"
     CDS             complement(6168..7586)
                     /gene="nhaC"
                     /locus_tag="ON430_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR3218895.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(7989..9212)
                     /locus_tag="ON430_000966"
     CDS             complement(7989..9212)
                     /locus_tag="ON430_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCR3218896.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(9243..10082)
                     /locus_tag="ON430_000967"
     CDS             complement(9243..10082)
                     /locus_tag="ON430_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR3218897.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(10099..10779)
                     /locus_tag="ON430_000968"
     CDS             complement(10099..10779)
                     /locus_tag="ON430_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218898.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(10781..11854)
                     /locus_tag="ON430_000969"
     CDS             complement(10781..11854)
                     /locus_tag="ON430_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3218899.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(11910..12148)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(12191..12424)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(12501..13172)
                     /locus_tag="ON430_000970"
     CDS             complement(12501..13172)
                     /locus_tag="ON430_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3218900.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(13259..15937)
                     /gene="mgtA"
                     /locus_tag="ON430_000971"
     CDS             complement(13259..15937)
                     /gene="mgtA"
                     /locus_tag="ON430_000971"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR3218901.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            16596..17033
                     /gene="ndk"
                     /locus_tag="ON430_000972"
     CDS             16596..17033
                     /gene="ndk"
                     /locus_tag="ON430_000972"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR3218902.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(17055..17921)
                     /locus_tag="ON430_000973"
     CDS             complement(17055..17921)
                     /locus_tag="ON430_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3218903.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(18081..20174)
                     /locus_tag="ON430_000974"
     CDS             complement(18081..20174)
                     /locus_tag="ON430_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3218904.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(20731..21354)
                     /locus_tag="ON430_000975"
     CDS             complement(20731..21354)
                     /locus_tag="ON430_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218905.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         6192 a         4414 c         3759 g         7007 t
ORIGIN      
        1 aaccaagcca atcccgaaaa tctgcacact gcttccttgt ctccacaagg tacagtctga
       61 atgcagcttt catcaatcga cttaattttt caatgcacgg atccctcact ttgtcctata
      121 tttgaaatta cgatagcata aagaaaatga ttttgtcaac tacttcggga ggatggtgaa
      181 aaatctgcta agtgtctgtc ctaagatttt tgttttttct aatgtttgcc tgtgttccat
      241 tgcttcttct aaacctattg gacccaaatg aatacttaag acaattggga gaaaatcaga
      301 aagtccttct agctcttggt ctctacttcc gcaaatagcg attattggtt ttcgatattt
      361 ttttgcaagc actgctacac cgtatggaac ttttccgtgg atcgtttgct gatcgatccg
      421 cccttctcct gtaatgatga catctgcttg ttcgatcttt ttttccaatc cagtgatttg
      481 gctgactaaa tcgaaaccag aaaccatctt cgctcccaac aaaaaaaggg ctccaccaat
      541 accgcctgct gctccacttc ctggaatatc atctaaatcc acctcccaga aatcttttat
      601 ttgctgagca atctttcctg cttgtttatc taagaaagct agcgtcgtat ctattcctcc
      661 tttttgtctt ccaaattgcc aagcagctcc ttgttgacct gtatatggat tggtaacatc
      721 tgcagcgata atcaatttta ctttagaaac aagaacgttt cttcgatcaa aatgaagttt
      781 tttagtagat aataaaggat ttccttctgt gtacccttct aaagaagcac ccaaggattg
      841 gagtaatcct agaccaccat cgctacaccc gcttcctcct aaagtgacaa taatttgttc
      901 tacttcttga agcagtgcat ctttaataag ttctcctaag gcataactgg aagcaagtcg
      961 gatagtttga tccgttggtt tatcaagtag atctaacccc aatgcttgtg cagattcgat
     1021 gactgcggtg ttttttcctt gccaagttgt caataaatag ctgatttttc gctgacggaa
     1081 taagagatcc atcccatcaa tggtacgcca tgttcccctg agcgcgtaat agattgcttc
     1141 tgcagtattt tctccgccat ctgcaatcgg cacattgatt tttcttcctt tacgatcttc
     1201 ccagccattt agaactgcct gatttagttc aaatgaagaa gcacttcctt taaatgaatc
     1261 aattgccgcc aaaatatcca tctgtatccc tcactttctt cttaagtaag acactttttt
     1321 tcattttttt caaacaaaac aatgctattt tcttccattt ttctatatac taaaagggaa
     1381 ctcattgaat aaaggagaga aaaaacatga aaatcgcaat agctggggcc ggagcaatgg
     1441 gcagccgaat cggattgatg ctacatcaaa gcggaaatga ggtcctcttg atcgatcgtt
     1501 ggcctgctca cattgaagcc attcgtacaa atgggttgat tgctgacttt aacggtaaag
     1561 aagtggtcgc aaaactgcca atctattcac cagaagaaat catcgaaagc aacgaacacg
     1621 tggacttgat cgtagcatta acaaaagcca atcaattaga tgatatgttt tgttcgatcc
     1681 aatccattat tactgacaat acttatgttt tgtgtttact aaatggatta ggtcatgaag
     1741 atgtactgga aaaatatgtg ccaaagaaaa atatcttatt tgggatcacg atgtggacag
     1801 ctggtctagc tggtcccgga aaagtcacat tgttaggtga tggagaaatc gaactagaga
     1861 acttggaacc agaaggcgaa gcatttacaa aaaaagttgt agaagtcttt caagaagcaa
     1921 atttaaatcc gatctatagc cataacgttc gttattccat ttggcgaaaa gcttgtgtaa
     1981 atggaacatt gaatggttta tgcacgattt tggattgtaa tattgctgaa ctaggcgcgc
     2041 aaaaagcggc agagagcatg gttcgaacaa tcgtatctga atttgcttct atcgcagcaa
     2101 aagaaggaat catactagat caagaagaag tgtaccaaca tattgcttct acttatgatc
     2161 ctgacaacat cgggttgcat tacccatcta tgtaccaaga cctgatcaaa aaccatcgtt
     2221 tgactgaaat cgactacatc aatggtgcca tttggcgaaa aggacaaaaa tatgatattg
     2281 ctacaccata ttgtgccttt ttgacccaat tagtccatgc aaaagaagga atccttggcg
     2341 cagaataaaa ggctctctga tgagaatcag ctgaatcagc agctggttct ctttttttaa
     2401 gcccaataaa agatcaaaaa gaaaacacaa ctctcatgat gccataagaa ttgtgcttgt
     2461 ttgtttcagt acataatctc ttcaaaatgt aggtttttta tttgttttca acagaggaga
     2521 ggtcatattc gtattgctca taaatagtgg aatcgttagt gataaatcgt tttgcccaat
     2581 tttctgcatc ataaaattcc aatagctctg ataggattgg cttaaatggt gtatgcagaa
     2641 attccggtaa ttcatctaaa tcacgttctc gaagcaaaaa aatattttca ggacgataaa
     2701 aatgataatg agcaatcgct tgattatccg tcactagttt cttttgaaaa tacatggatt
     2761 caaatgtccg caaagtcact ccttgctgac cctcccgcaa cagttccaga atcccgcgcg
     2821 attcccctgt ttttttcagg tactcttcat aagtaagata tccaggttgt tcattccctc
     2881 gatgtggaag aatgaaatag ttggtcgtaa catctctttt tataaattcc tgcttatagc
     2941 tttctgctcg ttcttttctc ccattattcg ttgcaccaaa aaatagatcc gtctttggtt
     3001 cgctagaaga aaaattcata tgtttcgaat aaaatgaact attatgtttc agcccaagtt
     3061 cttttgcttc caaaaaatca aaatgataaa tctcatcgat tgccggttca tcttgtgcct
     3121 gcttcagcat cgcctcgtgt tctgctttga agtgattcca aaaaaataac acaatttttg
     3181 cattggtaaa tgggcgcacg cgtcgagcca tctccatatc aaatccataa tctaaaaaaa
     3241 cgatcagctg ataatttgcc aactgttgtc tagctgattt aatgaattcc gactgtagta
     3301 aatcttttcc ggaccaaaca tctaaagagc ggatggaagg caaaaaataa agtgatttat
     3361 tttttgcaac tagcagtacg tcatttttgt tcatcttttc cgcctcactt tctctgtcac
     3421 tttttcctat gatagcctgt ttaaatagta aaaagccagt aaaacgtttc ctatatttgt
     3481 atgaaaattt aacatttgta tgaaaatatg atgaaataaa tcattcttct attaaaattc
     3541 gcctaaaagt tttctgaata gttgtaattg tctgaaaatt tgttatcatt agtcattgtt
     3601 aaatattgct atttattctt aatataaatt ttgattgtga atttaaaaga aagaggtcat
     3661 ttatggaaga taaaaaattg aaaaaacaac ttagctccag acatatcacc atgttagctt
     3721 taggtggagc aatcggtgca ggtctattta aaggaagtgg cgaagcgatt gggatcgccg
     3781 gcccttctgt attgattgct tttttgattg gtgggctgat tttatatatc gtgatgaaag
     3841 gtctgggaaa aatcgtttta agcggtggcg acacgcatca cggattatct ggtttgatac
     3901 gcccttatct aggtgctcat tctgctgatt ttactgattg ggtctactgg tctatgtgga
     3961 ttatcaatat catcgctgaa gctgttgcag ctgcttcctt tttgcagttg tggttcccgc
     4021 acattccagc ctgggtattt gtttttatgt tggctgtttt aactacgatc atcaatcttt
     4081 attcggtacg gttattcgca gaaacagaat attggctggc ttttgctaaa atcagtgtca
     4141 ttatcttact gatcattttt gctacttatc ttgtcggtca gcaaatgttg ggcacaggcg
     4201 ttttccctac ccttcagcaa ttgactgacc atggtgggtt tactcctcat ggtatgaagg
     4261 gaatcatcag ttctttgctt gtagttatct attcttacgg cggttctgaa ctgattgcta
     4321 tcacggtaag cgaagcagat gatcctaaac gtgcgattcc taaagcaatc aaaggtgtca
     4381 tgggaagaat catttccttt tacatcattc ctttgttttt gcttttgatt ctttttcctt
     4441 ggaatacttt agcaggcacg aatgtcagtc cttttgtgat ggtatttgaa aaaatgaata
     4501 tcccatttgc agcggatatc gtcaattttg tgattgtcct tgctttgttc tcatctatca
     4561 attcgggtgt gtatgcatct tcaagaattt tgttcttccg tttgaaggat cgaaaaggtt
     4621 cctctagaaa actagctgtt ttaaacaagc atcaagtacc gcaacgtgct gttttatttt
     4681 gtaccagtgt tctttatctt ggtgtagctc tttcttattt tgtcggtgac aaattatttg
     4741 gctatctggc aggttccctt tcgtatacag tattattgat ctggatcatt atcagtgcgg
     4801 catcctttgt actggcacta agaaaaggaa cattatggga taaaggaatc agcttttttg
     4861 cattagctgt cttaggattg attttccttg gtatcctgtt cacgaactcc attggggtaa
     4921 ctgtattgac tggcctgctt tacctgttca tttatttcag ttatcaaaaa aagaatgatg
     4981 cgttcgtatt gacgaatgaa tgaaatcgat agaaaatttt attttattta tgtggaatag
     5041 atggagctat tgaagaaagg agttgtgaca aaagtcttgt tgcaactcct ttttccgaat
     5101 aaacagtctc atgacctctt ggtaaacggt gttcaaaaac cctctgtgca aaataattga
     5161 ttgttctcca tgctcatgac atgatagaag aaaggaatga aaaaaaggag tttgttttaa
     5221 atgaaaaagt attggaagtt tttgttgatg atcggtgttt caacggtagt tatgtttggt
     5281 atgatgtact tgaatgtcta tgcgctcgat catatcttct tcagtcgtac acgagtattc
     5341 atggcgttga tgatgggtgc agtcatggca gtcatcatgc tgttgttcat gtggaaaatg
     5401 tatgaaaaca aaaagataaa tgttatgatc ttagccgcta gtcttcttgt ttttgccggt
     5461 tccttgttta tggtacgaag ccaaactact gtcaacgaca cagcttggat gaaggccatg
     5521 attcctcatc attctatcgc tatcctggca agtaaacgag ctgatttaaa agatcctgaa
     5581 gtaaaaaaat tagcggaaga aatcattcag gcacaagaag aagagatcga caaaatgaaa
     5641 aaaatgatca aagaaatgga atgaccatta tccgtccaaa taaaaaaaga ggatgttcca
     5701 gaagttatca gcttcaagaa ataagaagaa gctacttctg gaacgccgtg aataaggggt
     5761 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata gcttctccta
     5821 tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacaccg tttattaaga
     5881 ggttgtaaaa tcagcgttct gacctgagta ttagagagac aaatggaaaa atagttcctc
     5941 atattttgcc attttttgag ctaataccga gggtcagctg attgaacacc gtttatcgag
     6001 aggttgtgaa aaagtcgctt agtttcgcgt attaagaaaa attttgtgaa atggctttcc
     6061 acatttttgt ataaatttgg cttaataccg agaaactggc ttttgaatac cgtttattcg
     6121 taaaagggat tgtgacaaaa gtcttgtcac aatccctttt tatgttttta ttgttctaat
     6181 tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt tttttgagat tccccaacct
     6241 gtgaaggcac tgaccatcga aaacacagga gaaagcaaac tgaaaaatac gaatggcaag
     6301 taagtgagtg taggaatacc aaatgtactt gctacgaaac ttccggcaat tccccaaggc
     6361 actaagtagt tgattactgt tccaccatct tctagcgttc tacctaatac agttggatca
     6421 aatcctgctt tttcataagt ttctttaaat gcattaccag gcagaataac agataggaat
     6481 tgttcaccaa taaaaatatt tactccgata cttgtcatca atgttgtaaa aatcaaattt
     6541 ggtgtgttcg tcatctttct tgatactcgt tccatcacgg ttgagatcaa gccaaattcg
     6601 atcaacaatc cacctaatga caaagttaga ataatcaatg acacagtagg catcatactt
     6661 tcgattcctc cgcggctaag cagtgaatcg atttgttggt ttccagtatt tgaaacaaac
     6721 cctgattcga tgaccccagc caacttcgtt aaggaagttt gtgggttttg aataaaaatc
     6781 attcctactg ctacagcgat attcaacaaa atcgttaaaa ttgccggcac ctttttccat
     6841 gcacacagta acattaaaaa taaaggaatc agtgaccaga ttgaaatagt aaagtttgct
     6901 tctagaacat cagtcacttc acgaatagat gaaagacttg cggctttact tgtgtgtcct
     6961 aaaatcatga ataaaaagaa agaaacaaca aatgccggga cagttgacca catcatatgc
     7021 ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg ctagatttgt tgattcggat
     7081 aatggagaca tcttatctcc aaaaacggca cctgaaacaa tagctccaac aaccagcgct
     7141 ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt tgacataaca
     7201 gtaaaggcac tccctacagc gctacctacg attgcacaca caacaaatac agaagggaca
     7261 aaccactgga cactgatcaa tttgaaccca atgaccatta gagttggaat gatccctgct
     7321 tgtatccata cagcaatcaa tgaaccgact aaaataaaaa taaagattgg gatgattcct
     7381 ggtttgatgc cattgatgat tccttcgtgc agccaatctg tcgggtatct ttttatcact
     7441 gcatacaaca tgaccattcc gatcgctgct aagatcgtga tattaggtga gacctttaat
     7501 ccgacaacgc caatcgcaat gcctaaaaca ataaccatta atacaagtgt tgcttctaaa
     7561 aaacttggtg ttcctttttt tcccatgata atctctcctt ttttagttgt tcttatgttc
     7621 taaaaaagtt aagatatcgg attactccgc cgcaattcat ccgcgattcc tcctttataa
     7681 aaataaaaag acctgtttta aactaaggac gaatgactcc gcggtaccac cttaattaaa
     7741 aacagatctg tttttcactt tcaaactttt attcaaatgt atggactttc catgtaattc
     7801 gtcaattagg cttgatcggt tcacagcgac caccgacttt ctgaacaaaa cacctaaccg
     7861 ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc ataagatcat ttgtcaatga
     7921 aaaagaaaat attttttcat ttttctgaaa attattttta taactttcac acacaagctt
     7981 attttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc ttgatgttct
     8041 tgcttgattc tttctaatag ctttggctct ttcaacagat caagagctgt attggcgagt
     8101 agttctgctg caatcgcaat agaagcaagg cctttgtcac ttttagcagc agctttgaat
     8161 tcttctgtgt gtccggcaat atatgtctca gagattgcta ccgtaggctg gattgttggt
     8221 atgacttggc tgacatttcc tacatcagaa gaaccgagac ttgttctttg ttccgtttct
     8281 atttcttcat ctggaacacc tgcttctttt gcatgctgga agaataagtc atcaaaagaa
     8341 ggagtaacga tgacatcatc aactgcattt tggaacaatc caaactcaaa agtcgtgccc
     8401 gtacttatcg cagcacctcg tacgatattt tccacttttt catatacttt atctaatgtt
     8461 tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat gacgttggca
     8521 gctactcctc cgtctgtaat gatcccatgg atattgacat cttttggcaa ttgcaagcgt
     8581 agagcgttga ttccattaaa cacttggatc aatgcttcca aagcatttac tcctttttca
     8641 ggtgctgctg ctgcatggga agcgacaccg taaaatttga tgtcgactgg atcattcgct
     8701 aaagattctg tcgttttccc ataacgaaaa gctggatgga cgcataacgc tacatcgaca
     8761 tcctcaaaaa aaccttctcg aacaaagctg cctttagctg atccgttctc gcccccttct
     8821 tcccctgggg tgccatatac acggatttct ccaccagttt catcaatgaa ttgcttcact
     8881 atgctggcgg ctaatacgga gtaagtacca aacaaattat gcccgcatgc atgaccaata
     8941 cctggtaaag catcgaattc tgccaaaaag gcaattgtgg gaccgggttt attggaaata
     9001 taacgtgcat caaaggctgt tcggtgtcca gctacttctt tttctaccca aaacctttct
     9061 ttttctaatt gtttaatcaa cgctgctgag gaataaaact catagttact tacttcagga
     9121 tgttcgtgga tatctagagc aagtgcttga tattctggta aacgatattg gattagatcc
     9181 ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc tagtcatttt
     9241 tcttattttc tcccgaatgt ttcccatgca ggcacgcttg atccttttga tgttttctcg
     9301 attactttga ctgtgtcttc tgtttgataa gcgtctacta ttttttgata cgttttattg
     9361 ttttcatctt cttttcttgc aacgatgata ttcacataag gacgtgcatg gtcatccact
     9421 ggttctaaga agatggcatc agaagcagga ttaaatccag catctacagc tacaccgcta
     9481 ttgatcactg aaatgtctac atctgacaaa gcacgtgctg tttgggaagc atctaattca
     9541 gaaatgtcta gattttttct attctcggta atatcagaaa cagtgggcgc ttgtcctttt
     9601 gccttatcca ctttgatcaa tccagcagtt tgcaataaca gaagagctct gccgccattt
     9661 gtaacatcat tagggattgc tacttttgct ccgtctttga tatcagctac atccttcaaa
     9721 gtgttagaat aaattcccag cggtgcattg acagtatttc cgatcgatac taaatcagtt
     9781 ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt caaatcaatc
     9841 gatccatctg ctaaagccac atttggttga ttgtaatctg taaatttcac gtattccaaa
     9901 ttaatgttct cctttttcaa acggtctttc acgttgtccc atacatctgt atcttctcca
     9961 ataatcccta gttttacgat agtttctttt ttgtcagttg tatctgcttt tccttggcta
    10021 caccctgcca accaaataat tgctacaaat gtaatgacta caaaacgaat aatttttttc
    10081 attttttctc ctccactttc aatgtgtaat ttttttaatc agttgattac tgataaattg
    10141 acttaaaaaa actaagatta gaatcaataa tgtagccaca atcgttacat ctgtttggaa
    10201 tcgattatac cctctcgata tagcaagatt ccctaatccg cctgcaccta ctgctccagc
    10261 catggtagtc agtccgatca cgttgatgat agtcaacgcc gagacccgaa tgataccagg
    10321 caaaccttct aacaaataca cacgtaaaat gataccgatt ggactggttc ccatcgcttc
    10381 tgccgcttcg atcaccccag gatcaacttc tagcagtgcg ttctcgattt gacgagcaaa
    10441 aaacggaaca actccaaaaa tcaatggcac taaagctgct ttcgcaccaa tcgtcgttcc
    10501 aactaagaaa cgagtagtca atgctaaaag agaaagcaag ataatgaaag ggatggaacg
    10561 aatgatattg atcagcttat caagtatttc aaatagcaga cggttagcta gtaatccatt
    10621 agggcgtgtc actactaacg tcacacctaa gatgattcca atcatcccgc caatgaatgc
    10681 tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg acaaattcat ccggaatcaa
    10741 ataaacattg ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct ccttcgatca
    10801 ctttgatacg actatctaaa tcgttttttt gtaaaatatt tatttttaca ccagatttga
    10861 tcaaataatc caatgctttt ttccgctgtt tggtttctcc agctaaagta acgatcaaac
    10921 tccctaacgg cgtttcttgt aataactcta cattgccata taaaatattc gctgttactt
    10981 gatatttgct gtacagatga gcgattagag gttcgttcgt ctgatttcca atataactta
    11041 attccaccag ccatttattg gcgatctgat cggcaaaagc agaatgggca atgatcgttt
    11101 ctaatgcttg gtcaagatga gtagctgttc gaataaaatc tttggtcaat tcttcttcag
    11161 gattgctgaa gatctggatg ctgctacctt tttcaacgat tcttccatct tccataaccg
    11221 ctaccttgtt gcagatttct ttgacaactt gcatctcatg agtgatcagg acgattgtta
    11281 aacctaactg gcgattcaat ttttttaaca atgcaaggat ctgaagggtc gttttcggat
    11341 caagtgcact ggttgcttca tcacataata ataccttagg ttcattcgcc aaagctcgtg
    11401 cgattgctac tcgttgtttt tgtccccccg acagttgttt aggataagca gatgcttttt
    11461 cttccaaacc gacaagttcc agtagttcat tcactttttg acgacgttcc tgtttcgatt
    11521 ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt tccattagat
    11581 tgaaatgctg aaagatcata ccgatggcct ttctttcttt tcgcaattct ttgggagaga
    11641 gctgactcag attcgtctga ttgacaatga cttctcctgc tgttggtttc tgcagcaaat
    11701 tcatcacacg aactaacgta cttttccctg cacctgagta accgacgatt ccataaacat
    11761 cgcctttttc aatcgttaaa tctacagaat ctactgcctg tatctctttt tctttttgtt
    11821 tgaaacatac agaaacatct cgcaattcaa tcattaaaat tcctccttat ctaaaaaaag
    11881 cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt gttaccacct
    11941 catgttcaag ctgtgttcac acacaactcc tcttcaagta cgatcatact ctagcaagat
    12001 atcggtcgca gccgtcatcg ccttacatct atgctcggga tgaattgctc gatggccatt
    12061 ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa aggttcttct
    12121 atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt actaacataa
    12181 actatgtcag taaaaggacg acgtatcgcg gtaccacctt ttttcggaag tttccttccc
    12241 tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga acagttccga
    12301 atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc ttacttgact
    12361 cttctcacca gccagagcct ctctgcaaag tttccaaact tactttcctt ttcaacgaaa
    12421 tcgatatttt gttgttggga ttaatctaca tgaggaaaac tgaaaagtca agtacgaaat
    12481 tcaaattatt ttttctatat ttatttttct gtttttttat ttaacatttc aataacagtt
    12541 cgaagatctt ttccttccgc aagaagtttc aatttttcct tttcttcgac ggtataattt
    12601 cggacaaaac tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga agcagctgtt
    12661 gctcctacta aagaatcttc cactgcaaag gctgaactag cttctaatcc tgttcgttcc
    12721 aaagctgtca gataaggaat cggtgaaggc ttgttctccg acacttcgtc tccgaatgta
    12781 aacgtgtcca agtaatcagc taatttaaag tgatccaaga tatctgttgc tcttttcgta
    12841 acagtggtcg tagcaatgcc tagtttcata tgacgttctt ttaagtctgc caatatttct
    12901 tttgcatatg gttttaagtg gatggctccg cttgtcagct gttgataaaa atattcttca
    12961 cgcttcgctc ggacttcctg tactttttca gggctacctg ctgctttcac taaaatatca
    13021 aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttcttttgg gatatggata
    13081 tcaaattgct gaaatcccca aagccagcca tcacgatacg ttttttctgt gtcaactaac
    13141 aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc caaaacaata
    13201 cacatagttt agcacgactg gtccaaaaag aacagaagca aagaaccagc cttctcgttt
    13261 ataagagttc ttggaatcga cggatataga ttttcttcac agctgtcaca agcgccagat
    13321 aagcgatgac tgtagcaaat aaccataacc agtaattgcc aggaagtggc atcaagccta
    13381 attcagcacc aaaacttgta aaaggaagga cagaaccgac aataattcct aatgtagtga
    13441 taacaaacat ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt gtacgcagtg
    13501 catggagcac taaggtttgt gtccatagtg attcgacaaa ccatcctgca tggaataatg
    13561 cgatgaaaat cactcgctgt gaactatcca atgtatggaa acttccgccc actacagcag
    13621 gacaaataac aaaatacatc aacaagtaag tagtaatatc aaatatcgaa cttgttggac
    13681 caaagtaaac catgaattcc ccaatactag atgcattcca tttttttggc tcgtccaaat
    13741 attctttatc catccgatcc catggcaagg aaatgcagga aacatcatag atcagattca
    13801 aaaacaaaag ttgtaaaggc agcattggta aaaatggcag gaacgtactt gcaatcagca
    13861 cggaaaacat attgccaaaa ttagaacttg ctgtagcctt gatatatttc atgatattcc
    13921 caaaagtttc tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct ttttctaaaa
    13981 ggatcacgtc tgcagattct ttcgcaatat ccacagctgt atctacagaa ataccgatat
    14041 cagccacttt cattgcagga gcgtcattga taccatctcc taaaaatccg acagtatgac
    14101 catttttacg taaagctgtc gtcaaacgtg ctttttgctg aggattcaat tttacgaata
    14161 cttcatggtc ttcagctact tgtgctaatt catgatcact catttccgag attttctcac
    14221 cggtaaccaa ttcattgatc tctagaccta cttgacggca cactgaacgt gtaactaatt
    14281 cgttgtcccc agtcagaact tttacagcta caccatgttc tttcagagct ttcaacgctt
    14341 gtttcgttgt ttcttttggt ggatcaagga aagcaagata accaatcaaa accatgtctg
    14401 actcatcttt gactgaaaat tctccaacga cgcttggatt ggtcttttgt gcgacaccaa
    14461 tcactcgtaa gccatcttca ttaagtgact gtacttttgc aagtacggct tcttttcgtt
    14521 cttttgttaa tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg ttcagcattt
    14581 cttcaatcgc gccttttgtg atcatttgtg ttttaccagc tggatcagca accacaacac
    14641 tcatacgacg acgttcaaaa tcaaaaggaa tttcatccac tttttgataa ttgatcttat
    14701 ctgtatctaa agtttctgtt gcttcatcaa tgatagccac atctaacaaa tttttcagcc
    14761 ctgtttgata gtagctattt agatacgcat ggcgcaaaac acgatcatct tcatttcctt
    14821 cacaatccat atgatattca agaatgattt tatcttgtgt caatgtcccc gttttatccg
    14881 tacataacac atcgattgca ccaaagttct ggatggcatt caaatttttg atcactgttc
    14941 cttttttggc catcacactt gctcctttga ccaaattcgt cgtaacgatc attggaagca
    15001 tttcaggtgt tagaccaact gctacagata gtccaaaaag aaaagcttct agccaatccc
    15061 cttttgtaag cccattgatg acgataactg ctggagccat caatgacata aatttgatca
    15121 gtagcatgga tgttttatgg atgcctaatt caaagcttga ttgaattggt ttttcagata
    15181 gcgtttctgc tacatgacca aatagtgttt catttccaac agaaacaatg attccttccg
    15241 cacttccact gatgacatta cttcccataa agacaagatt ttcgtaactt gtttcgcttt
    15301 gctgatcgtc ttcacatact ttcgcatgct tttctactgg atagctttcc cctgtcaatg
    15361 ctgcttggga aatgaataaa tcctttgcgg acaataaacg catatccgct ggtatcatat
    15421 cccctgctga aagccgaaca agatctccac agacgatttc ttccatagga atctctgtgt
    15481 actttccatt acgttttaca gctgccgtta ctttcacaag tgatttcagt ttttcagccg
    15541 cttgattgga acgtacggac tgaatcaacg tcattgtccc gctgacgata accatagtga
    15601 agataatggc tacaccgaaa agatctttat ctccaggagc tgctaataca tattccgtga
    15661 tgaatgaaat aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg tatgctttca
    15721 atatttctac aatgaatggt gtttttttgc catatgaaat cgcattttca ccgtaatttt
    15781 ctcttgaacg ttcgatttgt tcttctgtca atccatttgc tgatgcatgg aatagttcaa
    15841 ataattcttg tttcgttttt ccagcaaaag ctgcatagat tttttgtgta tctttctttt
    15901 tttccatctc aatctctcct ttgtgtccaa aagacagctg ttctcgctgc ttttggtctt
    15961 tagctattca tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag atagataata
    16021 gccctggcct ttcctctaga tccttgtttt tcgaatgact gtcatccatt tgttctcatc
    16081 tcctttacta agtaatttta attcatgact tccaatcatt tttcgaatag ttatttttcc
    16141 aattagacca cctccttttt atttttcacc gatggggatt tgcggcaaca aaaaaacctc
    16201 tcgccaaata ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc tcagatctca
    16261 tcgttgagtt ttgacactgt acaacgtaaa aagcagatac ctgcttttag ctaccttatg
    16321 aaaagcctta tctcgacaat tcctgttcta cccattggcg tctctcgacg tttttgggca
    16381 gtagcctatg tttgtatagg agcctcacct aacaaatctt attcggtttt ccttcgttaa
    16441 tataacaact aaaaaagagg atgtcaaatc aattttccat gtttttcata ctttttttaa
    16501 aatacacacg ttatattcct gtctcacgcg gttttatttg tctcattttc cacctcccgc
    16561 tataatagaa atatagtagg aaacgaggtg ctgttatgga aagaacactt gttattatca
    16621 agccagacgg cgtgagacgc catttagtcg gaagcatcat ccaacgtttt gaagcaaagg
    16681 gactggccat tgctgaaatg aagtttgaaa ctatgactcc tgaattagca aaagaacatt
    16741 atcagcactt gactgagcgc agtttttttg atgaactgat cgattacatg acttccggtc
    16801 ctgtcgttta tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt aagatggttg
    16861 gagcaacaaa agctgccgat gctgttccag gtacgatccg aggagactac gcgctgccgg
    16921 ggactgaaaa catcattcac gcttctgatt cccgtgatgc tgcggtaaaa gagatcgccc
    16981 gctttttccc gccattcgat ataaacaaac cgtctgaaaa agcggcgaag tgatacaaat
    17041 tctaaactgc tggtctagtt gctgggttca gcaatggcca gcagtttttt ctctaaggac
    17101 ctccgttgat tctttcctaa catatattcg agcactgtga agaattcttg gtagtctttc
    17161 gtcttttctt cccctaaagc agcagaaatc aatctttgat aataatctgc catcaatgtt
    17221 tcatagatca ctgtacttct ttctttatgt gctaagctcc gtttcatatc ttcattcaca
    17281 ctacgtgcct ttttcaaccg accattttct atataaaacg aagtcaggtt cattaaaaaa
    17341 gtaaagagat actgctcatg attgttcaac tgtgctaatc gtcgcattct ttttttgatt
    17401 ttcaaataat tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc agctagaata
    17461 gatagttcaa attgaccata gtctggtatc tgcatcaagt attcttcaat gattcggcat
    17521 tcataacgta aaaattcaaa ttctgtctca gatacttctg ttttttcgac catgtgaaga
    17581 ttcaaaacca aataatacag atcttctgtt ttcttaaaat gcctacggtt attgctgatc
    17641 tctttataag gatcaaagct gtcttcttgt acccgatcta gcattcgtct ttttttcttt
    17701 aactttattg gttcatccag ctggtttcgc atccattcat attctgcaag tgtcatgttc
    17761 attcgctcta aaaatgatct caatgttaca aatgacattt ttttcactga atttgtctca
    17821 atcccgacta gagaagtctt aaaacagatt ccttccgcta attgggactg tgtcaacttc
    17881 ttgttgactc gaagtttctt gatcaattca ccatcataca taatagttct cctttttaat
    17941 ttaaattttt ccacattcaa taaatagata cgttaaaaag gaaaaataca cgaaaaaaat
    18001 aaaaaaatgt ttttttcata aaaaatacca accttcaagc attagaatgt tgtctaacta
    18061 ctgaaggttg gtttgattgt ttattgatta tttttttgct gttcaacgtc tgttcttgtt
    18121 actggatata agttggttgt tgttttatta ccattttttg aaaagacact tgtcgaatgt
    18181 tcgccttttt gcttttcgat tgcttcatta cgttccgctt catttttata gtcatatttt
    18241 ttcggatcaa tcgtctcaaa tccgtttggc acataaaacc gcaagagatt ttcctgattc
    18301 agcttatctg aaagacttaa cgcagtcttg actttttcct gatctttgga gatttcctca
    18361 tcgattcctg ctgctttcgt atcgatgatt tctcctgtct gattattgta agcttttcct
    18421 cctaggacgg tgtatttggg tgtcacaaag ttcccatttc gaaaagcaac tacttgatca
    18481 tgctgagaag ataataagtc tgtaccaaaa tgaagataat ctttatcatc gattcccaat
    18541 agatgtagta atgtcggcaa aacatcgatt tctccaccgt attcatgatt gactgtccct
    18601 ttagtataac caggcatatg gatcatcaat ggcactcgtt gcatctgtgc gttgtcaaat
    18661 tcatcccatg tatctggatc tttaccaaga gcgcttgcta aatctttatt atctgtattc
    18721 gagatcccaa aatgatcccc gtaaatcaca aagattgaat tttggtaaat accagatttt
    18781 ttcaaatacg taaaaaattg ttccaatgat tgatccagat aatgtgccgt tcgaacataa
    18841 ttatctactg tgctattgcc agtattcaaa gatggaaagt cgaaattctt gtcatccgta
    18901 taataaggcg tgtggttggt tacggaaaga aatttcgtgt aaaacggctg ttgtagatgc
    18961 tcaagatatt gggcagattc tctgaataaa tctttatcta atatcccata acctaacgtt
    19021 tcgtcggatt catcaaaata agatcgatca aagaaattgt cataacctaa gtttttatat
    19081 acatgatctc tattccaaaa actagctaca ttcccatgga aaacagcgct tgtatagcct
    19141 tgcttttgtc caaggatcgc tggtgcggct tgaaagacat tgtctgaccc aagttctgtg
    19201 aataatgagc cttgaggtaa accaaatgtg ccagtttcca acatattttc agcatcactt
    19261 gtctttccct gtcctacttc atgaaagaaa ttgtcaaagc tgatcgttgc ttgattctgg
    19321 aaaatactat ttaaaaacgg tgtgacttct tgtccgtcta ccttcatatt aatcaaaaac
    19381 tgctggaaac tttctaaatg gagaacgatc acatttttcc ctttagcgat cccaaatgtt
    19441 tcgggatttg gctctgcata atgtttcttt gtataatcta aaacttcatc aatcccatta
    19501 ctgcttgcat gcgcgcgaac actgctagtc attcctgttt tgattccgtc ataaatagta
    19561 tacgcatcga ttcctagata tttgacaata tatgaacggt caaatgttcg ctgcagcaat
    19621 tgtggacggt tggcttcgct cagcgctaga tttcctgaaa aaataagccc tgaaagacag
    19681 gtaacagcga tagccatcgg tttgccgaca gggttgcttt tgataggtat tttcttgacc
    19741 ttcaaccaaa ccagtagccc aatgaacaca gcgatatcca gccagtagat gacatcatgg
    19801 ggcttcatca aagaaaagga gcttcctgac aatccttgcg acactttcga aaagcctaat
    19861 acagatttga ctgtaatgaa atctgtgaat tcccgataaa aaataatatt caagtacaac
    19921 aaaagcgtat ttaaaatatc aatgatcaaa agaatagcta acgctggctt tggcttttta
    19981 atatacaaag aaaggccaaa aaacaataag gtagtcgcaa ttgggttgat ccacaagata
    20041 aagtactgga tcgtaccttc cacacctaat gaaaaatctg catagtaggc aatgatcgtc
    20101 ttcagccaga aaaagaaaac agctgtcaaa acgattgcta aacgattttg gcacaatttt
    20161 ttaagttgtg tcacgtatat cacttctctc tattcacatt ttttcttatt ttgctcctga
    20221 aaaatgaaaa tttcctaaaa aagttgttat cttaagattt ttttattctt tctaaagatt
    20281 attccgtcca cttttcacag cataaaaccg ctgcgatgta agcgattaaa ctaatgaaga
    20341 gataaaagtc ttgtggtgac cgcttcactc cacggtattc aaaagccagt ttctcggtat
    20401 tcgtacaaaa aaagaggctg tgacaaaatt cttgtcgcag ctccttattt cttgatgcag
    20461 gacagctttt tcacaacttc ttccttaaac ggtgttctcc tgccaactcc tcggccacaa
    20521 gtcacaatat ttataaaaca tgagaagctg ttttttgaaa ttgttccttg tggctcagag
    20581 ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac cttcggtatt
    20641 agctcaaaaa acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg
    20701 tcaaaacgct gatttcacaa cctctgtttt tcattgtcca taggattgat attgtccttg
    20761 tgatgactca ttcgtaataa ttgaggcttg attttcttca cttcccgtta tcgtttgtcc
    20821 actgccagaa tctgaaggac ttgttcctgt cgactcgttt ttttgagaag agacagttgt
    20881 tttcttgact tgttcatttg tctttttcca ttccgctaaa cgatcggcga ccactttttt
    20941 gtctactttt tcagaataag cggtgatcac ttttccattt ttcaaacggg ctactttgtc
    21001 gccatcatga gtcaaaagca aaacggtcaa atgcttgtct ttaaattgat attgtctgag
    21061 agaaatcgca ctaagtacgt catcgatatt aataaattga ttttcttctt ttccttttgg
    21121 caaataaatg tccaattcgc ctttttcgta attggcagtt attttatact ccgcccacca
    21181 ataagttgag aattcttttt tgaaattatc tactgattct ttgcttgcta cgattttttc
    21241 tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa aatgctgcgg ataaaataag
    21301 cagtaagaaa aaaccgaata accaaaagag aagaaacggt ctttttttcc tcatgctttc
    21361 ctccactcct at
//
LOCUS       DAJSMI010000032        21351 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJSMI010000032 DAJSMI010000000
VERSION     DAJSMI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.032"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..196)
                     /locus_tag="ON430_000976"
     rRNA            complement(<1..196)
                     /locus_tag="ON430_000976"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            937..1626
                     /locus_tag="ON430_000977"
     CDS             937..1626
                     /locus_tag="ON430_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3218906.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1613..2821
                     /locus_tag="ON430_000978"
     CDS             1613..2821
                     /locus_tag="ON430_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3218907.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            3115..4386
                     /gene="serS"
                     /locus_tag="ON430_000979"
     CDS             3115..4386
                     /gene="serS"
                     /locus_tag="ON430_000979"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR3218908.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4935..6419)
                     /gene="guaB"
                     /locus_tag="ON430_000980"
     CDS             complement(4935..6419)
                     /gene="guaB"
                     /locus_tag="ON430_000980"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR3218909.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANTLLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6924..7619)
                     /locus_tag="ON430_000981"
     CDS             complement(6924..7619)
                     /locus_tag="ON430_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCR3218910.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7709..8809)
                     /gene="ychF"
                     /locus_tag="ON430_000982"
     CDS             complement(7709..8809)
                     /gene="ychF"
                     /locus_tag="ON430_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR3218911.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8818..9009)
                     /locus_tag="ON430_000983"
     CDS             complement(8818..9009)
                     /locus_tag="ON430_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCR3218912.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(9030..9920)
                     /locus_tag="ON430_000984"
     CDS             complement(9030..9920)
                     /locus_tag="ON430_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR3218913.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9907..10674)
                     /locus_tag="ON430_000985"
     CDS             complement(9907..10674)
                     /locus_tag="ON430_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3218914.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10686..11399)
                     /gene="rsmG"
                     /locus_tag="ON430_000986"
     CDS             complement(10686..11399)
                     /gene="rsmG"
                     /locus_tag="ON430_000986"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR3218915.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11631..13532)
                     /gene="mnmG"
                     /locus_tag="ON430_000987"
     CDS             complement(11631..13532)
                     /gene="mnmG"
                     /locus_tag="ON430_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR3218916.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13549..14946)
                     /gene="mnmE"
                     /locus_tag="ON430_000988"
     CDS             complement(13549..14946)
                     /gene="mnmE"
                     /locus_tag="ON430_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR3218917.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            15245..15628
                     /locus_tag="ON430_000989"
     CDS             15245..15628
                     /locus_tag="ON430_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218918.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15796..16554)
                     /locus_tag="ON430_000990"
     CDS             complement(15796..16554)
                     /locus_tag="ON430_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCR3218919.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16572..17390)
                     /locus_tag="ON430_000991"
     CDS             complement(16572..17390)
                     /locus_tag="ON430_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR3218920.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17387..17743)
                     /gene="rnpA"
                     /locus_tag="ON430_000992"
     CDS             complement(17387..17743)
                     /gene="rnpA"
                     /locus_tag="ON430_000992"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR3218921.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17861..17995)
                     /gene="rpmH"
                     /locus_tag="ON430_000993"
     CDS             complement(17861..17995)
                     /gene="rpmH"
                     /locus_tag="ON430_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR3218922.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18518..19852
                     /gene="dnaA"
                     /locus_tag="ON430_000994"
     CDS             18518..19852
                     /gene="dnaA"
                     /locus_tag="ON430_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR3218923.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            20050..21180
                     /gene="dnaN"
                     /locus_tag="ON430_000995"
     CDS             20050..21180
                     /gene="dnaN"
                     /locus_tag="ON430_000995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR3218924.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         6088 a         4346 c         3916 g         7001 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactcc tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aatgtttgtt gtcttgaatt gaatcaagac gccctacaca
      301 tttggtttgt cttactttgt tcagttttca aaggtctact tcatcaggag tgtttcccga
      361 ttcgtttttg gctgccgtag caacttttat atcatatcag agagacaagt aattgtcaac
      421 tcttttttaa aagttttttc gatttcgttt ttcgaaatct tccagcaact catttatcat
      481 aacttatcgt ttgttatttg tcaagaactt ttttgaagtt tttttcgata gaatcaagct
      541 tcgcaagtgg ttctttttta acgactcagt tatcttatca tgtcatttcc aacatgtcaa
      601 caacttattt tttattattt aataagttat tggtgttctt ttgaacaaat aataatatat
      661 caacatcaca tgtgaaagtc aataagaaaa tgcttattta tcaacatttc tagcttacat
      721 gatcattggt tagttctggt cctttttcgt tcgtctttat aactccgaga gacgtaaaat
      781 ccttttgttt tcagtttaat gttcatgttc ttgtcttatt cacaccaact agatgtctca
      841 ccttctaaaa ttttccttaa caaagtgctg cttttatcta tttctgcttt tttttgctag
      901 aataatagta acaatatgac tctggaggat ttatttatga aaattttgat tgctgacgat
      961 gataaagaaa tcgttgaatt attaagtatc tatgttcaca atgaggggta tgaagcagta
     1021 aaggcttatg atgggaaaga agcattatct aaaatcagaa caatccctga tattgaatta
     1081 cttattttag atatcatgat gcctgagatg gatgggatgc aagtagtgaa agagctacga
     1141 aaagaatccc aaataccaat cattatgctg acagcaaaaa ctactgatat ggataagatc
     1201 aaaggactgg tcgctggtgc agatgattat gtgaccaagc catttaatcc gttagaagta
     1261 atggctagag taaagtcttt attacggcgt acaaaaatgc agattgcccc agatcagcct
     1321 gatgtattag aagtaaatgc attaatcatc aataaagact ctcatgaagt taaaacaatc
     1381 gatgggaaag aaatccagtt aacagctttg gaattcggta tcctctactt gcttgctagt
     1441 catcctaatc gagtatttag cgcggatgaa atttttgagc gtgtttggaa acaagaaagt
     1501 attgtctctg ctaaaactgt catggttcac gttagtcatt taagagataa gattgaagaa
     1561 gcaactggtg gtgaaaaagt cattcaaacc gtttggggag tgggctataa aattgacgca
     1621 agatagtaaa cgagcaactg aaaaacgccg tcggatcaat ttgacttcaa aagaaatcag
     1681 cgaattgtta gcggaaggaa tcatcacgat tatccttttg cttctattaa atgtttctat
     1741 tctggttgtt atcagttcgg tgatcaacag ttcaccttct ttgacgaatg cgatttggga
     1801 ttcaaagaat atttttgcag aacgattgaa cactgatttg ttctggaacg gccggaattt
     1861 tattattccc tttttctttc tattagatat tggtgttttg tattggcgct tgattcgaag
     1921 atatcgccaa atgcaattgc ggcatatcat cagcgaactt cactacatcg caaacggaaa
     1981 ttatgatcat cgtatccctt ttgaactgag tggtgactta agccgagtgg taacaagtat
     2041 caatggattg gtggatagta ccgtagcagc gattgaagac gaacgtaaaa tcgaaaaatc
     2101 aaaagatgaa ttgatcacga atgtcagtca tgatatccgt accccactca cgtccatcat
     2161 tggataccta ggactgatag aagatggcca gtatcactca gaagaagatc ttttaaagta
     2221 tacgcatact gcttatatca aagcaaagca gatgaaatct ttagtggacg acttatttga
     2281 atacactaag gtacgccagc ctgctgtccc ggtgaatttt tctgcctttg acatgatcca
     2341 gttgatcgaa cagttagcag ctgattttga gttggaagct tctaaaaaaa atatccaaat
     2401 acttgttcaa tcaaaagtag actcccttat catggatggc gataccgaaa aacttgtacg
     2461 tgtcttcaat aatcttttga cgaatgcgtt gaaatacgga aaaggagcaa cgaagatcgt
     2521 aatcgaagta gaacgtatcg gttcagaagt tgttgcaaca gtcaaaaata acggagcaat
     2581 gatcccacaa caggcaatcg ataatctatt cgaccgtttt tatcgtgtag aagaatctcg
     2641 ttcacaagca actggaggaa ctggacttgg tttagcaatc gctcaaagca ttgtggcttt
     2701 gcacggcgga tacatttatg caaaatcaga taagcaatgg acttctttca tcatgcacct
     2761 tccaatcaag aaaaatgaaa aattacctat tgatacacag gaagttattg acgaaagctg
     2821 aaatattcgc tattcttatt acaattatta gaataaatga aaaacacaga acccagtaca
     2881 ctttattctt tttcagagag ctgatggcag gtgtaaatca gcaaaggata atttggaagt
     2941 tccagttcgt ttacgtgccc tttcgaaata agaaaggtcg gttcaacagc cgttatgtgt
     3001 tcaacaagtg cagctgacgc tgaacttggg tggtaccgcg aatttcagac tgattcgtcc
     3061 caagaataat gggatggatc agtctttttt tattttcaaa aaggaggaaa tcatatgtta
     3121 gatgtaaaaa tgatgcgaca aaatattgaa gaagtacaag aaaaattgat gactcggggc
     3181 gtaaaaaaag aaacattagc tgaattcatg gaactagatg aaaatcgtcg taagctacta
     3241 gtcaaaagtg aagaattaaa aaaatatcga aatgatgttt ctgctgagat cgcccaattg
     3301 aagcgtgcaa aagaagatgc ttctgctaaa atcgctgaaa tgaaagaagt cggtggcaac
     3361 atcaaagcat tggatactga aattgctgaa gtggatgaaa aattgaaaca aattgcaact
     3421 actttgccaa accttcccca tgattctgtg ccagttggaa cagatgaaga ggacaatgta
     3481 gaaatccgtc gttggagcga accacgtaca tttgcttttg aaccaaaacc acactgggaa
     3541 attgcagaaa atcttgatat tttagatttt gaacgtggcg caaaagtagc cggtagccgt
     3601 tttgtctatt acaaaggatt aggtgctcga ttagaacgtg ctgtctacaa cttcatgctt
     3661 gatcagcata tttacgaaca cggctatact gaaatgatca ctccttatat cgtcaacagt
     3721 aaagcgatgt ttggtacggg acaatttcca aaattcaaag aagatgtttt ccaattggag
     3781 aatactgatc taacattaat cccaacagct gaagtaccat taactaacta ttataatgat
     3841 gaaattttag atggtgcaga tttacctatt tatttcactg cactaagccc tgcttttcgt
     3901 tctgaagccg gaagtgctgg tcgggataca cgaggactga ttcgtcttca ccaattcaat
     3961 aaagtagaaa tggtcaaatt ttcggatgta gagcattctt acgaagaatt ggaaaaaatg
     4021 acagctgatg cagaagatat cttgaaaaaa ttgcatttgc cttatcgtgt gatggcctta
     4081 tcaactggag acatgggctt ttctgcagct aaaacttatg acttagaagt atggatacct
     4141 gcacaagata cttatcgtga aatcagttct tgttctaact gtgaagattt ccaagcacgc
     4201 cgcgcaatga tccgttatcg cgataatgat ggaaaaatcc actatactca tactctgaat
     4261 ggttcaggac ttgcagttgg ccgtacagta gctgccattt tggaaaacta ccaaaatgaa
     4321 gatggttctg tcacagttcc agaagcgctc gttccttata tggggaatct gaaagttatt
     4381 aaataaagag gctgtgaaaa agccgcttag tttcgagtat taagaaaaat tttgtgaaaa
     4441 tggctttcca catttttgca taaattggac ttaataccga gaaactggct tttgaacacc
     4501 atttatcgag aggttgtgaa atcagcgaat taacctgagt attagagaaa aaaatggaaa
     4561 aatagtccct catattttac cgtttgttga actaataccg agggtcagct gattgaacac
     4621 cgtttagtgg agaggtcgta agcctacccc ccagctccta gttacaagga caaattcaaa
     4681 aaacagctct tcatatttta tgaatgttgt acttgtgatt cggaggtggc aagggaacac
     4741 tgtttattca aaaagaggct gggacagaag cgtttaactc caagaaataa gaagaaattc
     4801 acgaaaattg cttttcaaat ttttgtgaat ttcttcttat ttccgaagga gttgcttcta
     4861 cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt gttctttact tttgtcccac
     4921 gctctttaat gtttttaaga ttcaactgaa tagtttggtg cttcttttgt gatttgtaca
     4981 tcatgagggt gtgattcttt taatccactg ccgctcattt ggataaattg cgcttcatca
     5041 cgtaattgct gtagattggc tgccccaaca taacccattc ctgaacgcaa accgccgatc
     5101 atttggaaaa taatatctgc aacacttcct ttataagcaa cccgaccttc gattccttct
     5161 ggtactaatt tatttgcttc attgacacta ccttggaagt aacggtcttt tgaacctttt
     5221 tccatcgcgc ctaatgagcc catacctcgg tatgttttga atcgacgtcc ttggaagatt
     5281 tcgaattcac ctggtgattc atctgtccct gccaacatac ttcctagcat cactgcatgt
     5341 ccgccggcag ctaatgcttt gacgatgtca ccagaatatt tgatccctcc gtctgcaata
     5401 atcgctttgc catattgacg agcaactgat gctgcatcat aaatagctgt gagctgaggt
     5461 acacctacac cggctaccac acgtgtcgta caaatcgatc ctggtccgat accaactttt
     5521 acgacatcta cacctgcatc atataaagct tttgtcgctt cagccgtcgc aacgtttcct
     5581 gcaatcaatg ttgcatctgc aaaagttgaa cggatttctt gaatttttcg gatcacacct
     5641 gcactatgtc catgagctgt atcgatgatg attgcatctg cccctgcttc taataaagta
     5701 ttcgcacgct caaatgtatc gcttgttaca ccaacggctg ctgctacaag caatcgacca
     5761 tgttcatctt ttgctgcatt tggaaattcg atcactttct cgatgtcttt gatcgtgatc
     5821 aatccgctta aacgaccttc gttatctacg attggtaatt tttcgatttt atgtttttgc
     5881 aaaatctttt ctgcatcttt tagagaggta cctactgggg cagtcactaa gttgtctttt
     5941 gtcatcacat cagcaatcgg catgtgataa tctgttacaa aacgcatgtc gcggtttgta
     6001 attatcccta ccaattttct gttttcaagt gtttcaacga ttggcacacc actgatacga
     6061 tatttgctca tcaattcttc tgcgtctgct acaagatgct gcggcgttaa gaagaacgga
     6121 tcaatgatta cgccactttc agaacgtttg acttttctta cttcatccgc ttgctggctg
     6181 atcgtcatgt ttttatggat cacacctagt ccgccttgtc ttgccatagc aatggccatc
     6241 ttgctgtcgg taacagtatc catacttgca ctgatgattg gaatattcaa cttaatattt
     6301 ttagctaatt gtacgctcat atccacttcg tttggtaaca cgtgactttc tgctggtatc
     6361 aataagacat catcaaaagt aaatcctttt ttcgtgaact ttgtttccca gttggacatt
     6421 tttagtacca ctcctctaat ttagtttatc tattaaaata attgattttt tcatttaagt
     6481 caatgatcta cttggaatca agttagcttt ctcatagaat accaatataa agcgtttaca
     6541 tctgtgtgta ttaaaagaga ttgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc
     6601 aatcaaaaac agcttctcat gtttttgaat tggttgggtt tattaccgca gatgcaaact
     6661 tttgaacacc gtttagtgga gaggctgtga aaaagctgct tagtttcgag tagtaagaaa
     6721 aattttgtga aaatggcttt ccacattttt gcataaattg gacttaatac cgagaaactg
     6781 gcttttgaac accgtttatc gtaaaaaggg actgtgacaa gacttttgtc acagtccctt
     6841 actattattt aactatggat aaacaaaatg aggttcttta ctacccctag atatctagca
     6901 aaatagctga tctttttgct agtttatctt cttgggacgg ataaacttcc aataatcccg
     6961 tattttttct tcaagtagta acggattgct aaagcagttg ccccgataat gataactact
     7021 actggatcta atactggatt gataactggt ggcaatagtg ttgtcgcaga aaacagtacg
     7081 acccaaagaa gtgtcgttcc acctaggata agcaatgtct tacccattcc tggtttcttg
     7141 cttcgatctg ctccgggata ttcatattga tagatgtatt tgtacatcat ataaaagacc
     7201 cagccaccga ttgcagaacc tagaacgagt gtagaaaagc cataagctgg cgttgttcca
     7261 cgtgaaaaca atccggtaat cgcaatcatg atacttaata gaccaagcaa cagcaacgta
     7321 ttatctaacc acatcaataa aggggtggat gaatttttta cttctgctgg cttattgatg
     7381 attgcttccg ttctttcaga gactgtccca aacaattgac gggctgttct tccgcctttt
     7441 tgttctgcaa ctaaagtagg cagaatgtca tgaagggctt ttactttttc ttcttcagac
     7501 aaattcgctg cttctaaaga ttttttcaaa tcaaaaatgt actgttgatt tcttttggtt
     7561 aattttgttt ctaaactatt gttttctttt acgattgctc gtaattgttc tggttccatt
     7621 atctaatagt cctccttagc cgttcctttt ctcaacggtc gtcacaaaag acattctacc
     7681 atattctaca gctagtttga tagccctttt acacattaaa gcggaataac attacgtcgc
     7741 cgtcttgtac gacataatct tttccttcta atcgaacacg tccagcttct tttgctgcat
     7801 gcatgtttcc gtaatgatcc aagtcttcaa aagagacagt ttctgcacgg ataaatccgc
     7861 gttcgaaatc tgaatggatg attcctgctg cttgtggtgc tttgattcct tttttgaatg
     7921 tccaagcacg tacttcttgt tccccagcag taaagtaagt agctaagcct aacaaatcat
     7981 aagcagcacg gatcaactga tccaaacctg attcttcgat tcctaaagct tctaaaaatt
     8041 ccgctttgtc ttcttcatct aattctgcga tttcttcttc tgcacgagca ctgaccacga
     8101 tcacttctgc attttcttct gcagcaaagt tgcgcacttc ttgtacataa tgattcgcgt
     8161 ctgcatctgc tacgtcatct tctgagacat tggctacata aagaattggt tttgtcgtca
     8221 ataaaaatag tgatttaacg attttttgtt cttcttcagt aaattcgatc gttcgggcag
     8281 attttccttc ttccagtaca ggtttgattt tatccagtac agctagttct gccactgcat
     8341 ctttatcttt ggttttagca acttttgcta cacgagtata gcgtttgttg atagattcta
     8401 agtctgctaa cactagttcc aagttgatcg tatcaatatc ttctagtgga tcaacacgtc
     8461 cttctacgtg agtaatatta tcatcatcaa aacagcgaac aacgtgacaa atagcgtcta
     8521 cttggcggat atggcttaag aattgatttc ccaaaccttc tcctttactt gcgcctttta
     8581 cgattccagc aatatctgta aattcaaaag tcgtcggaac agtttttttc ggatgtacaa
     8641 gttctgttaa gcgttgcaaa cgccagtcag gtacttctac catccctacg ttggggtcga
     8701 tcgtcgcaaa gggatagttt gctgcttctg ctcctgcttt tgtgatcgca ttaaacaaag
     8761 ttgattttcc tacgttgggc aacccaacaa ttccagcagt caatgccata aatttattca
     8821 ctctttctct gcattttttt caattacttt ttttaatttc ttttcaaaat ctcgacgggt
     8881 catcatgacg atatgtccgc aattcataca tctgattttg atatctgcgc ccattcgaat
     8941 gacttcccag cgattcgtct ggcaagcgtg aggttttttc atttcaacga tatcgtgcaa
     9001 gtcgtacata aaaatagcct cctttttgtt tattcttcat caaattgaat attcaagata
     9061 tcaagaattc gagtcaaatc tttttgagaa agatattcaa tctcgatctt acctttccca
     9121 tttttttctt ggatagctac acttgtacca aatttatcca tcaaacgatc ttcgctttct
     9181 cggatataat atggtttttc ctttgctagc cttgggattt ttttcttttc ttttccatca
     9241 ttttgattta actcagaaac aagctgttcc agttggcgga cagttaaatt ttcagttact
     9301 gcacgattgg ctaatttcaa gatcaatgtt ttatttttta aacctaacag cgtacgggct
     9361 tgtcccatag ataaacgctg atcttgtacc atttccttga caagatccgg taaagaaagc
     9421 aagcgcaaat aattggcgat ataaggtctg ctctttccta aacgactagc tacttctgct
     9481 tgtgttagtt tcaggttttt catcagcatc tcatatgctt ctgcttcttc taacggattc
     9541 agatcttctc gctgcaagtt ttccagcact gctacttgca tcatcgcttc ttcgtcaaag
     9601 tctctaacga tagcagggat cgtttctttt tcagctagtt tggacgctcg aaaacgtcgc
     9661 tcccctgcaa taatctcata tccttttact gcggaacggc gtacgatgat gggttgaaag
     9721 acacctgatt gttcaataga attagctagt tcctgcaaag atacttcttc aaatgtttta
     9781 cggggctgat acggatttgg tcgcagctca ttcaatggta gctggacaac ggtatcatct
     9841 tttacatcca ccgtctctaa gttttctaaa tcttgaaaca gtgcatcgat tcctcggcct
     9901 aatcctttac ttttcttcac gacttaccac ttcctttgcc aatgcttggt agacttcagc
     9961 tcctcgagaa cgtggatcgt agtcgatgat cggttttcca tgactaggtg cttctgataa
    10021 acgaatattt cttggaatga tcgtttcgta aactttttcg cggaagtatt tacgtacttc
    10081 ttctacgact tcatttccca gatttgttcg tgcatcatac attgtcagta agactccctc
    10141 gatctctaat tctggattaa agtgtttttg gactaaacga accgtattca gtaattggct
    10201 taatccttcc aatgcgtaat actcacactg aacaggtatc aaaatcgaat cacttgctgt
    10261 aaatgaattg attgtcagat ggccaagaga aggcgggcaa tcgatcaaaa tataatcata
    10321 ctgactgctt acttctgcta aagatccttt taatcttgat tctcttgcca tcattgacgt
    10381 caattcgatc tctgctcctg ctaattggag agtcgcaggc acaatactca aattttcgtg
    10441 ttcagtgatc aaagttgcct catcaattgg caattcattg actagaacat catagatgtc
    10501 tcttgtcaca tcaggttttc tgatcccgac accactggta gcatttcctt gtgcatccat
    10561 atccactagc aatacttttt tgccaaggct agcaagacaa gcacctagat tcaccgtcgt
    10621 cgttgttttg ccgacgccac ctttttggtt cgcaacagat attatttgtg ccatctcttc
    10681 tcctcctatt tgattggctg cttattaggc agtccgggct ttcttgggta tttctttggg
    10741 gttggtttct ttttttgaat aacgatgata tgccgttgat cagagacatt cggcaaggta
    10801 accgcctctt ctttgaggaa tttaccacct aataaagcaa tcgcttgttt tgcttctgtc
    10861 aattcctctt cactttttgc tgctttcaat gcataaaaat agccttcttg tttagccaag
    10921 ggcaaacaca attccgctaa cacattcaaa cgagcaaccg cacgagcagt gacaaggtca
    10981 aatgatgcac gaaagtgagg attttgccca aatgtttctg cccggtcatg atataaggaa
    11041 gcatcgattc ctaataatcg ggttaactca gataagaagg ttattcgttt attcagagaa
    11101 tcaacgatcg ttatctgcag ttctggaaag gcaattttta aaggtatgct aggaaaccct
    11161 gcccccgaac caacatcgca taaagataaa ggttgcgtaa agtctacttc aaatgctaat
    11221 gtaagtgaat cgtaaaaatg cttcagatat acgtcttttt catccgtaat agctgtaagg
    11281 ttcatctttt cgttccattc gactaatgtc cgataataaa tagcgaattg attcatttga
    11341 tgatcggtaa gctctatccc tttttctgcc aaggattggc gaaattcttc tggtatcata
    11401 aatattcctt tcattgtgaa acagcaattg tttcacagat tctctcccta ctttaacgca
    11461 aaatctctag acatgcaatc cttccatatg gattcttaaa aatttgaaaa gtaagaaaag
    11521 ccgatagact ttttctttct agtatgactc ataaaatata cacagaagag agaactagct
    11581 ttgagcaatt tctgcaaaaa ctagttctct cttctattta ttattacgta ttacgctggt
    11641 tcactgactt tcgcaatctt cccttgttcg atatagacca ttaggatact taggtctgca
    11701 gggtttacgc cactgattcg gctagcttgg gcaattgttt ctggttggat tttctgcaat
    11761 ttttgttttg cttctgttgc taatccgttg attgctgcgt agtcgatgtt ttctggaatc
    11821 tttttcgctt ccatgcgttt taatttttct actttttcca tggctttttt gatgtagcct
    11881 tcatatttga tttggatttc tacttgttcg atttcttttc gtgtcaattc ttcattttct
    11941 ggaataaacc gtagaagttc tgcataagta atctcaggac gtcttaaaaa gtcgcttgct
    12001 agtacaccat cttttaatgg tgccgcatta atcgttgcta aaaagtcttg gacttcttct
    12061 gttggtttga ttcgtacatt catcaaacgt ttgatttctt gttcaactgc ttcttttttc
    12121 ttcaaataat tcttgtattg atcttctttt actaagccta cacggtgacc catttctgtt
    12181 aatcgcaaat ctgcattatc atgacgtaaa agcagacggt attcagcacg tgaagtcagc
    12241 aaacgataag gttcgttcgt tccttttgtt accaaatcat cgatcatgac accgatgtaa
    12301 ccgtcacttc gtttgatcac taaaggttct tttccctgaa ctttcaaagc tgcattgata
    12361 ccggcaatca gcccttgtcc agccgcttct tcataaccgc ttgtaccatt tgtttgtcca
    12421 gctgtataaa gattttcaac gactttagtt tctaatgtcg gacggagctg gtgtggctct
    12481 actacgtcat attcgatagc atatcctgta cgcatcattt ctactttttc taatccagca
    12541 atagaatgaa gcatgttgac ttgaacatcc tctggtaaag aagtagataa accttgaaca
    12601 tagacttctt ccgtatttaa gccttctggt tccaaaaata attgatgacg tggtttgtct
    12661 gcaaaacgca cgatcttatc ttcgatggat ggacaatagc gagcgcctac cccttcaacg
    12721 atccctgtaa acattggggc acgatgtaag ttgtctcgaa taatttggtg agtcgtctca
    12781 cctgtatagg tcagccagca agattcctga tctagtttat aggcactatc tggtgtgctg
    12841 aagctaaaat gattcggctc tttgtcacca ggctgctctt ctgtaacaga atagtcgatc
    12901 gtacttgatt ttactcgagg cggtgttcct gttttgaacc gttcgatagc aaagccaagt
    12961 tcttttagat gattcgctag tccaatagaa ggttgagagt tattcggacc tgatgaatat
    13021 ttcaattcac cgataatgat ttctccgcga agtgctgttc cggctgtaat aacaactgct
    13081 ttggcccggt agctcgctcc tgtggacgtc actactccat gacaaacacc atcttcgacg
    13141 atcagttctt cgacgattcc ttgtcttagc gtcaaatttg gttctttttc gatcgtatgt
    13201 ttcatttctg ttgaataagc atgtttgtct gcttgtgctc gcaatgctcg gacagctggg
    13261 ccttttcctg tgttcaacat acgcatttgg atataggttt tatcgatatt cttacccatt
    13321 tctccgccta acgcatcgat ttctcgaacg acaacgcctt tagctggtcc gcctacagaa
    13381 ggattacatg gcatgaatgc taccatgtcc aaatttattg tcagtaataa tgtttttgat
    13441 cccatacgtg cagcagcaag tgcagcttct gaaccggcgt gtcctgcgcc aacaacgatc
    13501 acgtcatacg catctgcttc ataatgttcc aatcgtttgt tcctcctttt attttcctaa
    13561 acaaaattga ctgaataatt gagtgattag ctcatcttgt acactgtcgc cgacaacttc
    13621 tcctagatag tcccagcaac gcgtcatatc tatttgaacg agatccacag gcattcctga
    13681 atcaatacct gcaatgactt cttcaagtga gagggaagct ttttctaaca acgcgatatg
    13741 gcgggtatta gaaacataag tagcgtctct ttctcctgtt tctccgctga agaataaatc
    13801 agaaatagct gattccaaac gatctagtcc gtcgttttta gctacggaaa ttgaaaaaac
    13861 tggttcattt tcgatcaatt tcttcaattt ttcttgttcc agctgtgctg gcaaatctgt
    13921 tttatttaga agaatgatcc gtttcaatcc acttgtcgct tccaataatt gttcatcttc
    13981 tgctgtcagt ggttcgcttt ggtttagaac aagtaagatc aagtctgctt ctgctaatgc
    14041 ttttcggctt ctttcgaccc cgatccgttc caccacatct tctgtttccc gaattccagc
    14101 tgtatcgatt agcttgagcg gtaccccccg aacattgaca tactcttcga ttacatctcg
    14161 agtcgttcct gcaatatccg taacgattgc tttttcttct cttaacaaat ggtttaacaa
    14221 acttgatttg ccgacatttg gccggccgat gattgccgta cttaaccctt cccgcagaac
    14281 cttcccttgc ttggatgtag caagtagcgc ttggattctt tgttggatca tctgcgcttt
    14341 ttcaagcaat aacttcgtag tcaattcttc gacatcatca tattctggat aatcaatatt
    14401 tacttctact tgtgctaatg tctctaaaat ctcttgtctc aatgagcgga tcaaagcaga
    14461 cagattgccg tctaactgat taagagctag tcccattgct ttgtctgttt ttgcccgaat
    14521 caaatccatc acagcttctg cctgtgaaag gtcaacacga ccattcaaga atgctcgttt
    14581 tgtgaattcg cctggctcag ctaatcttgc accttctctt aacaatagct gcaaaatttg
    14641 attgactaca acgatcccac cgtgacaatt gatttctact acatcttccc gcgtaaatgt
    14701 ctttggtgct ctcatcacgg agaccatgac ttcatcgaca agctgattgc tcttgggatc
    14761 aacgatatgt ccataatgga tcgtatgact tggtacttct gataatcgtt tgtttccaga
    14821 ttgataaact ttatctgcta gttgcacagc ttgatcgcca cttaaacgaa cgatactgat
    14881 tgcaccttct ccaggtgggg tagaaattgc cgcaatcgta tcaaattcta aagtaatttc
    14941 agccattttt tcacttcctt tttcattttg cgcacaaaaa aagtgcccac tccacccttt
    15001 atgcacaata aacggtgaac ttcgcacttt gactgcttgt tcttgattaa attctggtca
    15061 tagattatca cacttgctat aaggaatcaa gtgtttaaaa caattagatg aagttgagcc
    15121 tgttctgctt ccattacctt atatttaatt gtccttggat gtctctttgc gttctttggt
    15181 ttattcgaag agaagatact gtataaacaa ggctttatct ttcttaaaaa aaacggtaca
    15241 ctaaatggta gcgctatcta aactatacac ttttgaaagg agaaaatatg tgagcccgtt
    15301 cagatctgct gcattggaag aagaaagtga ccctcggatc aacgatgtcg ttttatctac
    15361 agggaatatc aaccagaaat atatcgtccg cgatatcgtt tttgttgtgg aatgcttaga
    15421 agctgatttg tttgacccat cttttcccaa agatgacctt ttcttaacaa cgaagaggaa
    15481 attaaagacg aaggtacttg aatatggtgc gaatgcagta atcaactgtc attttgagta
    15541 tcagcaaaaa aaacgcgacg gaaagtattt tctggaactt tttgcttatg gaacagtgat
    15601 ccaatttatc caaacaacga ttggttaaat gaaacagaca aaaaaagcgg atcaaaactg
    15661 aagacagttt cgatccgctt ttttctatca aagttagtga ctacttcatc gtatactctg
    15721 ataagaatga tttctagtaa aatatacttg gtttgaaaaa ctttgtcaac gtgatttatt
    15781 caaaaagtaa tggaatcaat aatatttttt agcaggttct actactaaat aacgataagg
    15841 ttcctcacct tccgaatgtg tttttacatg ttcatctttg cttaatgcag catggatttg
    15901 tttacgttca aatgcaggca ttggttctaa gaaaacaggc tgccctgtcc gttttacttt
    15961 ttcagccgtg cgatcagcta aacgctgaat gatctcttgg cgcttttcac ggtaattgcc
    16021 tacattgact actacagaca gcttatttct tgctaaacga tggatgaaga cttgtgccag
    16081 atattgcaat gcattcagtg tttttccatg tttcccaatc agtatgcctt gcttttgtgt
    16141 ttctaaatgg aaaacaacca tattatttga ttctctttcc attttgacaa gagctggagc
    16201 atcaagctgt tttgaaattt cagtaagata aactgccaat tccgttaaag ctgcttcatc
    16261 acctagctca cttacttctt ctgttttttc ttcaagaatc gtttcgttga ctacctcgat
    16321 ctcagctgtt ggttcttctt ccaaaatttc ttctacagtc tgttctactg tttcagaaac
    16381 tttttcgctg atacttggct ggatcgaaag ccgtgcattc tttcgaccca ttcctaggaa
    16441 accttttttt ccttcttcca gtatttcgat ttcgacttgg tctttggcca aacccaaatc
    16501 agccagacct agttggacag cttcgtctat tgttgcagct tcataaatcg gcattagcat
    16561 tacctcccct attatttccg ttttttctta ggattcattg ctttcttaag agcacgttct
    16621 ctttcgcgga ttctccgtgc ttcttcttca cgctcttttc gaattttgaa agggttgttg
    16681 atcagcagcg tttgtacaac ttggaaggca ttggaaacta cccagtataa tgaaagaccg
    16741 ctggctaaat tgacacccat cactaaaatc attacaggca tcacgaaatt catgatcttc
    16801 attgatgcat tcgattcaat ttgactcatg ctagataaat acgtacttgc aaaagtaaag
    16861 attgctgcta aaatcggcaa aataaatgtt ggatccgcgt tccctaagtt caaccataag
    16921 aaatgccctt gtttcaactc tggtacacgt gaaatagatt gccataacgc catcaagatt
    16981 ggcatttgta ctaataaagg aagacaacca gcatatggat tgacgccatt ttctgcatat
    17041 aaacgctgct gctcttctct taacttactt tgtgtttccg gatctcttga actatattgt
    17101 tgctgcagtg cttttaattt cggttgtaat tcttgcgttt tacgcatgct ctttgtttgg
    17161 aaatgcatca acggcaatag gatcacgcgg atgatcagtg taaataaaat gatccctatc
    17221 cctgcattac caaatgacaa cgccttgatc gcctcagcaa agtaataaac aatataacga
    17281 tcccaaattc ctgtactctg tgcgctgact tccccagttc cgcaaccgga taagacgatg
    17341 accaaactaa cgataccggc catcaaaagt aaacgtttgt atttcttcac ttactctttt
    17401 cctctctgtc gattattttt gcaagtttta agacgtggac taaattggag cgaacctcat
    17461 cataagtcaa ccccttcaca cttggtctag caatcacaat aaaatccaac tccggatcaa
    17521 tttcttcttt tatagaataa actgctgctc ggatttttcg tttgacttca ttgcggttta
    17581 ccgcgttgcc gacctttttt cctacagata aaccaactcg aaaatgaggc tgtcccggtt
    17641 tttccaaacg gtaaacgacg aatttgcggt tagcaaaaga atctcccgta tggaacacgg
    17701 cttgaaactc tcgttctttt tttactcgat acgcttttct cattagtttt ctttcctttt
    17761 ctatctctca cttacgctca taatcatacc ataattacga ttgtcctttc atcatttccc
    17821 taaaaaagag tataaaaaaa accactgaaa ccccagtggc ctaagctgaa agaacttttc
    17881 ttcctttgcg acgacggcta gctaacacac gacgtccatt tttagtactc atacgtttac
    17941 ggaatccgtg aactttttga cgtttacgtt tatttggttg gtatgttctt ttcatatatt
    18001 ccacctccaa aatgttctta tctattttat aaacacagac atactttgat agtataacga
    18061 caaagtagtg attttgtcaa tataaaatag aagatcgatt gttaaaactt atccacaaga
    18121 ttattttttt ctatgggaat cctatgtgga aaaagttatg cacagggatt atccacagat
    18181 aataaaaggc tggttttaca cagaaaacac acgtgtaaaa aaagtaatcc acaatcaggc
    18241 gatttcctgt gtattccgtc tcctaactat tggtatatct gtatttttta tccacagacg
    18301 caagaggaaa tctattggtt tttacgagtt ttccacctgt gcattagtgg tgtggaaaac
    18361 tttttttgac aactgtgttt ttctcttttt tctttttgtc gattgtgtgg ataattgtaa
    18421 ttaaaaaatt tttttcttcg gtttctgtgg aaaactattg aaaaaaatgc taaattagta
    18481 ctagttattc actttttaat gaaggaggag gccttatatg gtatccctcg atgctttatg
    18541 gaatgaatta aaagcaacat accaaaagga tttatcgcct gctagctata atacatggat
    18601 cgaaacagcg aaccctcgaa cccttgatca aaaccagctc gttgttgagg ttcctagcaa
    18661 gattcataaa gaatattggg aaaaaaacct ggcgacaaaa atcgttgaag tcggttatat
    18721 gttatcagga aatgagatta tccctcgctt tattactggc gaagaagcag agcaagaaga
    18781 agttatagaa gaaaaaaatc caaaagttgt ggcacctagt ccgctaaaaa aagccatgct
    18841 gaaccctaaa tatacctttg atacgtttgt cattggtaaa ggaaaccaga tggctcatgc
    18901 agcagcgctc gttgtagcag aagatccagg ttctatttat aacccgctct tcttttatgg
    18961 aggcgtggga cttggtaaga ctcacttgat gcatgcaatt ggtcatcaaa tgctgcaaag
    19021 tcagcctaat gccaaagtaa aatatgtaag cagtgaaact tttgctaatg atttcatcaa
    19081 ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa tatcgaaatg ttgatttact
    19141 gctagtggat gacattcaat tttttgcaga aaaagaagct acgcaagaag agtttttcca
    19201 tactttcaat gccttgtaca atgaagggaa acaaatcgta ctaaccagtg atcggttgcc
    19261 aaacgagatt ccgaaactgc aagaacgcct tgtttctcgc tttgcatggg ggctgtctgt
    19321 tgatattaca ccgccggatc ttgaaacaag aacagcgatt ttacgtaaaa aagcggcggc
    19381 tgaacgtcta gagatcccag atgacacact aagctacatc gcaggacaaa ttgattctaa
    19441 cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct tttgctgcga tgaatagtga
    19501 agatatttcg actagtctag ctgcagacgc actgaaaacg ttaaaatcag gaaaaggaca
    19561 tcctcaattg tcaatcctgc agatccaaga agaagtggca aagtattatc acatccaatt
    19621 gaaagatctc aaaggaaaga aacgcgtcaa atctattgtt gttcctagac aaatcgctat
    19681 gtatttggca cgtgagctca cagataattc tttaccgaaa atcggagctg aattcggtgg
    19741 caaggatcat acaacggtta tccatgctca tgaaaaaatc cagcagctga tggattctag
    19801 tgtcagtatg caaaatgaag tatcagaaat caaaaacttt ttactaaatt aatttgtcga
    19861 ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa cgttcaaaag ttatacacac
    19921 tttttccaca gctgtttttt cgtttctctc ttacgttttt tgagttttcc acagaatcaa
    19981 catgccctat tacttttatt attcttttta actaataata aataaacaaa cattcaaagg
    20041 agatacatca tgaaagttac tttaaaccga gctagcttta tgcaggaatt gcaaactgtt
    20101 caacgagcta tttcaagcaa aaccacgatc cctattttga caggtgtaaa aatcacactg
    20161 acacaagaag gtttgacttt gacggggagc aacgctgata tatcaattga aacttttttg
    20221 tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta ctggttccat tgttttacaa
    20281 gcacgtttct ttagcgaaat cattcggaga cttcctgaag aaacatttac tttagaagtt
    20341 ttagaaaata aacaagtagc gatcacttct ggaaaagcga attttatcgt aaatggatta
    20401 gatgcagata actatcctca tcttcctgtt gtcgaaagcc ataaccagat gaaattacct
    20461 gtacacgtat tgactaaact aatcaacgaa acagtttttg ctgtctcaca acatgagagt
    20521 cgtccaatct tgacaggtgt gcatttcatt ttatctgata attctttatt agctgtagct
    20581 actgattctc accgtctaag tcaacgcgtg attccagtag aacaagcggc tgatcatttt
    20641 gatattgtta ttcctggaaa aagtttgatc gaattatctc gctcattaac aaatgaagaa
    20701 gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt tcaaaacaga aacgatgtat
    20761 ttctattccc gtttgctaga aggaaactat ccagatacca atcgtttgat tccttcaagt
    20821 tttaatacag aggttgaatt ttctgttcct agctttttag cggcgatcga acgggcttct
    20881 ttactttctc atgaaggccg taacaacatc gttcgtttat ctattcgtcc agatgcggtt
    20941 gttttatacg gaaattcgcc agaaatcggg aaagtagaag aaagcttgag ttatacagca
    21001 agcagtggcg atccattaga tatttctttc aatccggatt atatgaaagc agcgttgcgt
    21061 gcatttggag atatgagcat caaagtgaaa tttatctctg ctattcgtcc atttacattg
    21121 gaaccaacgg aagatggcgt tcaatttatt cagctgatta ctcctgtacg tacaaattaa
    21181 caatgatttt caatgttctt gaaaatttaa ttgaaaagaa atagaaaaaa taaaatttta
    21241 aacagaaggc cctaaaatct cttttaaggc tttctgttta tttttgttta aatcaccttt
    21301 tttagattaa atcgtcttat atggaaaaat atatggataa atttgtttta t
//
LOCUS       DAJSMI010000033        20690 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJSMI010000033 DAJSMI010000000
VERSION     DAJSMI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.033"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            155..1861
                     /gene="efbA"
                     /locus_tag="ON430_000996"
     CDS             155..1861
                     /gene="efbA"
                     /locus_tag="ON430_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCR3218925.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2019..2921)
                     /locus_tag="ON430_000997"
     CDS             complement(2019..2921)
                     /locus_tag="ON430_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3218926.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2918..3610)
                     /locus_tag="ON430_000998"
     CDS             complement(2918..3610)
                     /locus_tag="ON430_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCR3218927.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3653..4294)
                     /locus_tag="ON430_000999"
     CDS             complement(3653..4294)
                     /locus_tag="ON430_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3218928.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4332..4646)
                     /locus_tag="ON430_001000"
     CDS             complement(4332..4646)
                     /locus_tag="ON430_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218929.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4634..5986)
                     /locus_tag="ON430_001001"
                     /pseudo
     CDS             complement(4634..5986)
                     /locus_tag="ON430_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5983..6915)
                     /locus_tag="ON430_001002"
     CDS             complement(5983..6915)
                     /locus_tag="ON430_001002"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCR3218930.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6912..7688)
                     /locus_tag="ON430_001003"
     CDS             complement(6912..7688)
                     /locus_tag="ON430_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCR3218931.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7742..10927)
                     /gene="carB"
                     /locus_tag="ON430_001004"
     CDS             complement(7742..10927)
                     /gene="carB"
                     /locus_tag="ON430_001004"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR3218932.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10932..12008)
                     /gene="carA"
                     /locus_tag="ON430_001005"
     CDS             complement(10932..12008)
                     /gene="carA"
                     /locus_tag="ON430_001005"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR3218933.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12013..13293)
                     /locus_tag="ON430_001006"
     CDS             complement(12013..13293)
                     /locus_tag="ON430_001006"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR3218934.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13312..14238)
                     /locus_tag="ON430_001007"
     CDS             complement(13312..14238)
                     /locus_tag="ON430_001007"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCR3218935.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14251..15534)
                     /locus_tag="ON430_001008"
     CDS             complement(14251..15534)
                     /locus_tag="ON430_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR3218936.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="ON430_001009"
     CDS             complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="ON430_001009"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCR3218937.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16322..17233)
                     /locus_tag="ON430_001010"
     CDS             complement(16322..17233)
                     /locus_tag="ON430_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3218938.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17230..17694)
                     /locus_tag="ON430_001011"
     CDS             complement(17230..17694)
                     /locus_tag="ON430_001011"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR3218939.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17699..18397)
                     /locus_tag="ON430_001012"
     CDS             complement(17699..18397)
                     /locus_tag="ON430_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3218940.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18384..18770)
                     /locus_tag="ON430_001013"
     CDS             complement(18384..18770)
                     /locus_tag="ON430_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218941.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18917..20587)
                     /locus_tag="ON430_001014"
     CDS             complement(18917..20587)
                     /locus_tag="ON430_001014"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCR3218942.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
BASE COUNT         5760 a         4686 c         3658 g         6586 t
ORIGIN      
        1 cctaaaaatt tggatttcaa cgatagaaac actgtttttt atgtaattag tccaagattt
       61 ttctagactt ttgctttact attcggccaa attggttttt cttctttttt tcgttataat
      121 gaatatactt atgaaaagaa atgaggaatg aaccatgtcc tttgatggtg tatttaccca
      181 cgcgatggtc aacgaattgc gtgaaacgtt actatctggc cggatctcga aaatccatca
      241 gccttatgaa aatgaaatcg tgctggtgat tcgttcacgc ggcaaaaacc atcgattatt
      301 attatctgcg cacccaagtt atgctcgtgt acagattacc caaatcgatt atcagaatcc
      361 tgataatccg cctaacttcg tgatgatgct tcgaaaatat ttggatggcg ctattttaga
      421 agacatcgaa caaatcgaaa atgatcgagt gatccatttc cactttgcta aacgaaacga
      481 gttgggtgat ctacaaaata tcatattgat cgttgaatta atgggacgtc atagtacgat
      541 cgtcttagtt aatcgagaaa ctggaaaaat actggatgcg atcaaacaca ttggaagctc
      601 tcaaaacact tatcgttctt tgcttcctgg tgtagaatat gttgctccgc caaaacaaga
      661 ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt cccaaactga
      721 actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata tcgctcaaga
      781 gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg acgaattttt
      841 ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata aagatttttt
      901 cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag cttatccaac
      961 actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc gggcaaaaca
     1021 acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata aaaataaatt
     1081 gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc gtagaaatgg
     1141 cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag ttgttttacc
     1201 taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat tgacaccaaa
     1261 tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg ttaagctgat
     1321 tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg tcttatctca
     1381 actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga cttcagaagg
     1441 atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc agcctgatca
     1501 atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc aaaatgacca
     1561 gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa atatacccgg
     1621 ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag aagctgccga
     1681 gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg atcttgtaca
     1741 ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt atgaaaacca
     1801 aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac aaaacaggta
     1861 atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa ttagttataa
     1921 attgtttgct acaaaaagag gagttgtgac aaaattctgt cacaacccct cttttttgat
     1981 caacggtgtt caataaacag cgttctcatc tgcttttctt agctctgctg gtccagtaac
     2041 cgagtaagct ctttgataaa tctttgagcg atttcactga gcggacgttt cgccgaataa
     2101 atgatataaa attcatttag ttcgccttct gccaaaggga ttaaccgcaa gtgctcgcga
     2161 atccctccaa tcacaatacc agatcctgaa gcgtaagcat cagtttcaag aacgatattc
     2221 atcaaagtac ctcgatcact cgtataaatc actgtttctt gatcagggat ctcgataagg
     2281 tcttcataaa aatacgggaa attatttcca tcctgtgtaa aacgaacttg aggataagat
     2341 aacaattctt ctttggtgat ctctgcgtgt tcagctaatg gatgtccttg ccttaaaaaa
     2401 atatgcgttt tgaattttcc aatgctgcgg ccacttagct ctccttgttg taaatatctt
     2461 ctgataccag cttgattatg ctcattcata taaataatgc cgatctcact gtgctgctct
     2521 ttgacttctt cgatcactct cgctgttgtc gtctccatga tccgaaattg tttgatctcg
     2581 tctttgaatt cctcgagtag ctgtgcggtc aaaggaccta agaaatcata atgctgggca
     2641 gcgatcgaaa aattgtctct cttttcactc gttagatagt gagtttctaa tgtatcaagc
     2701 tggaccaata ttttctcggc acgatttaag aagtcttgcc cttcttctgt taaatatgcc
     2761 cctcgattcg ttcgagtaaa cagcgtgatc cctaactcgg tttctaattc tttgatacca
     2821 gctgaaagac ttggctggga gacaaacatt ttctttgccg cttctctgaa actaccattg
     2881 tttgctacag cgaccacgta tttcatctgc tgaatattca ttgctcaaac tcctcgaaat
     2941 aataaatttc tcttccattc aagggctgga ttggtcggtt gttcggtcga gcatactgac
     3001 catcgaaggg gagaagaaac tcttggttca gttttcctac atgatccatt acaaaccatt
     3061 ggattggccc gctggtaggc ggagttgtca atgagccaat ataatggaaa tggctttttt
     3121 gctccggaaa gaacacatcc ggatttaccc aatgctcatg tttttctaag ttccaacttt
     3181 ccccattttc ttgattatac agccaacctt cctgtaccat tagctggtac atgacaccca
     3241 caactagatt ctcttttttg ctgttcatat gaacaaaatg aaattcaata tcttcctgtt
     3301 ttccgttgat gatatgttca gaaggcaagt ggaaatgaat atctgtcaaa taatagcgtt
     3361 ctttcttgaa ctctacataa ctgagttggt caaaaggtac taaatgaatc gtgtttttga
     3421 attccttgtc agtaaaccgt tgcttttgat aatgaaagga aagcgtgtct ccttctattt
     3481 tggtttcttc ccccttggtc aaatgaatag gcgactgcaa tggaaattcc gcacctctcg
     3541 caaaccaatc acataaactc gcccaatttt ccggaccttt gtctccagaa tagccccatt
     3601 cgacatccat attctttttc aactatactt ccccctgcat ctcatatcat ttttattttt
     3661 cttctttctt tacaatcgtt gagacggttt ctaaaaacgc ctcttttcca taaaattcct
     3721 ggttcaatag ttccggaaat atctctttaa agaaatactg aacagaaggc cgatgttcaa
     3781 agcttttaat cgtagtcact gcctcaatga acatctcctt ataaactttt tccgggttat
     3841 ttttgatgat ttcatcaaaa gattcataaa gaaggtcaac cttatctgaa atggctaata
     3901 gttctccttc tacggtttca tcttttcctt cgaacaaccg acggcgataa acttcttgga
     3961 actcagctgg aatctcttgg ttgatgaatt cgtccgtcat cttttcttca actgtttgca
     4021 gcatccctct taactctttg cttgcgtatt tcaccggcgt tttgatatca ccaataaaac
     4081 gctctgtgta gtcatgattc aatgattttt catagagcag tttccaatcg atcggttgtc
     4141 cattcgcttc ttcaatgtct cctagaactt gcgcaataga tgctacacga tacgaatgtg
     4201 ctgcaactgt atgctctgta tatttaaaaa atccaggtgc ccttgtgatc gtctctaaat
     4261 tgttcaatcc taaaataaat tcattcaacc ccataaaagt tacctccctt tgagatttct
     4321 ttttccgaat atcatatcat taaataactg atattcgcaa catttccgct ctttaccatt
     4381 tttgaaacag ctcccttacc aaaaaataga gaaaatctat atataataga aaaaagagaa
     4441 gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa aaaaatcaaa
     4501 aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct cgcaatcagt
     4561 ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat ttctctatct
     4621 tcatcaagcg cgcttaaatc tgccaatttt caggatcttt tttccattct tttagcagag
     4681 ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat acagaataat
     4741 tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct tttggcaact
     4801 catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt gctgcttcag
     4861 ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc atcttctgac
     4921 cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc ttacttcgaa
     4981 tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg attcctgctg
     5041 ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg cttgctaatc
     5101 cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta tcacagtaaa
     5161 ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa aatactgctt
     5221 caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt tagctccatt
     5281 ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt ggtcgaccta
     5341 taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact cttgtctgat
     5401 ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat tcgtctcctg
     5461 ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct aatcctgctt
     5521 ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg attagttgat
     5581 cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga agtagcggac
     5641 gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc atcatacgaa
     5701 taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat ccccgcattg
     5761 ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca ttatgaccag
     5821 cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc attccgattt
     5881 tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc tcttgcctag
     5941 ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt cacgttcctc
     6001 tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca ttcgcacagg
     6061 gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg tccctatagc
     6121 tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct gcacgccgcc
     6181 catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac gaatagcgac
     6241 aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg gttttcttgt
     6301 cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat ctgcccctcc
     6361 agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca atttgacata
     6421 gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa catcagaatc
     6481 tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca gctcgattgc
     6541 tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt cttcacaagc
     6601 accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg gaagaagttt
     6661 atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca tgccagatgg
     6721 tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg ttgccttgac
     6781 catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc caaaaccgaa
     6841 acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt tgaccgctaa
     6901 aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc gtcacaaact
     6961 ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc atagcaagcc
     7021 cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg cgaagaaaac
     7081 agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc tgtaggttcc
     7141 gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt tccatcgtca
     7201 gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac gacttcatgt
     7261 gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg ttttgataac
     7321 tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc tcctgtatga
     7381 agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg gtctccggca
     7441 gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat acggcaagta
     7501 tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa gtctgcacgc
     7561 ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag atcacctttc
     7621 aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc catcatttct
     7681 tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca tgtgctcgcc
     7741 gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc gcatctgctg
     7801 tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg atgatgaaac
     7861 cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact tgtgttttac
     7921 catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt ttcgctactg
     7981 tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt cctatcagac
     8041 tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt gtttcgtccg
     8101 caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct tcgaatgcct
     8161 tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc gatttcattt
     8221 caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca ggtgctttaa
     8281 catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc tttagtttct
     8341 ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc ttacttaaga
     8401 atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca ttttcatgga
     8461 tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag cggatcgtgt
     8521 aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac acagccattg
     8581 aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg atgagtactg
     8641 tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa cggtccacta
     8701 gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct aaatctcgct
     8761 ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga acaagaactg
     8821 gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca gtatccccta
     8881 aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga ttttccgcac
     8941 gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc aaaggttcgg
     9001 ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc tgttctagct
     9061 cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta tccgaaatag
     9121 aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg tatcctgcct
     9181 gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct tgtccgatac
     9241 ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa ctttcatttt
     9301 catattcata ggaactataa aaataaggtg tttttgattc gaattccgct gcacaagtgt
     9361 caaccatctt atatacagga atcaaatcat tttcttttct catctggcga acggcttctt
     9421 cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt ttcgctgttt
     9481 tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct acgatatgca
     9541 acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct tctatggtgt
     9601 accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct cgaaccactt
     9661 tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata tggtgtgtac
     9721 cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga ccgatagcca
     9781 tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt tcaaacttat
     9841 caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca aattctgcaa
     9901 aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc gctattttag
     9961 cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat gaacgagaaa
    10021 cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga gcgagttgga
    10081 cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca tcacgaagca
    10141 tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa tcacctgtat
    10201 ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta tcagccgaat
    10261 ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca atcaaacatt
    10321 gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct tcttcatttt
    10381 cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg acaggatagc
    10441 cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca gactcgggga
    10501 ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct gcttgatcga
    10561 tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata cctgatgctg
    10621 ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg agagcatctg
    10681 gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg atatatactt
    10741 gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact aaaaccactt
    10801 catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca aattctgcgg
    10861 cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata tctgtacgtt
    10921 tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc atcaaataaa
    10981 tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact gaaagcggga
    11041 taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg agttacgatg
    11101 agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg agaagtaaaa
    11161 tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg tccgaatttc
    11221 attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag acaaatacca
    11281 aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg cacatctttt
    11341 ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa aatcgtctcg
    11401 gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa ttctcgtaaa
    11461 atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc tggacttgga
    11521 taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt tgctttcagc
    11581 tgatcaaatg catgttcgaa ggaatctcca gcatcaacta tactagcttt catcgttccg
    11641 acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc aggaattcct
    11701 ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc tacacgtgca
    11761 tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc atctctattt
    11821 accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt aaaactttga
    11881 tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc tccgaagaca
    11941 ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa tataagcaaa
    12001 cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa aagtcatttg
    12061 tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg ataaaaagtc
    12121 ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag cgggggcacc
    12181 aaccgttagc gttcctgcat tcaaaccaaa aatctcagcc ggtttaactg ccaaccaatt
    12241 gatcacttgt tccaacgtga atcgaccagt ttctacaaaa tgagtataga tcagttgaaa
    12301 cgctgtttcg cttcctacga taccaaatgg tgatttcatg aaactttggc ttttttcttc
    12361 taatccatgg ggggcatggt ctgtagcgat gcaatcgatc gtaccgtcaa gcaagccttc
    12421 gatcaaagct tctctgtcct cccgtcctct tagcggcggg ttcattttcc aaaagccgaa
    12481 atcttcggga atatcttcgt cgatcaaaat aagatgatgg ggtgaaactt ctgctgttac
    12541 gtggattcct gctttttttg cgtctctgat tacacggaca ctttctttag tagaaacatg
    12601 gcaaacatgg taatgaacac cagttgcttc agctagcaac agatctctag caatttgaga
    12661 agactcggtt acgcttaaga tcccgggaag accaagttct tctgccttct tgccagcgtg
    12721 cataacgccg ccaaataata aagattcatc ttctgtatgg gctactaggg ctttattgtt
    12781 ttttgcagct tctttcatag ccagatacat cgtcccagct gtttggacac ctacaccgtc
    12841 atttgtgaaa gcaaaagcac cttcttcaat cagtgcttct tgatcaacta atttctcact
    12901 tcgcagattt tctgtgattg gcgcatactg caataccttc acaactgcat cttttctaat
    12961 gatgtcatat acttgtctca gcttttctgc cgtatcaggt acaggattta aattcggcat
    13021 cgcgcataca gtagtgaaac cgccacgagc agctgagcga gtcccagctt cgatcgtctc
    13081 cttgtatgta aatcctggct ctctcaaatg aacatggacg tccactaaac caggtgtgat
    13141 caattgtccc tttgcatcaa acacttcatc aaattcggct tctgagaatc ctgtaccgat
    13201 tgctttgatt cgcccatctt ctatccaaat ttctgctggt gtcgtcatat tttttcgtgt
    13261 attgatctga ccgtttttaa tgagtgtttt cattttttca tcctcctata attacgcttt
    13321 tccatttagc actgcttcta agattgccat tctgacgaat actccattgc tcatttgctg
    13381 aacgatccgc gactgtaatc cttccactaa tgaatcagct aattctacat cacggttaac
    13441 tggtgctggg tgcataatga ttgcatgttt ttgcaacttc ttcgcacgct caactgttaa
    13501 cccatgttcc tgatggtagc cttcttttga gaaactttct tttccatcat ggcgttcgtg
    13561 ttgcacccga agcatcatca tgacatcaac ttgatctagt agttcatcca atggcatata
    13621 atgtccgtac acttcgaatt catcgtcgta ccattctcta ggacctgaga agaatacttg
    13681 tgcgcctaga cgttttaaca tctgcatatt ggatttagct acgcgagagt gagtgatatc
    13741 acctacgatg gcaactttca gaccttcaaa atgaccaaat tcttcataaa tggtcatcaa
    13801 atcaagcaag cattgggttg gatgctgtcc gcttccgtct cctccattga taatcgaaca
    13861 ctggattgtt ttactttgta tcagttcgtc ataatagttc tcatcaccat gacggataac
    13921 cgctacgtct acgcctaatg ctgacatcgt cagtactgta tcgtatagtg tttccccttt
    13981 ttggacagag cttgtgcttg cttcaaattc aatcacttct agccctagtt ttttctccgc
    14041 tacatcgaag cttttatgtg tgcgagtact attttcgaaa aagaggtttg tagcaaagta
    14101 ctggcgcttt tgcggagtcc aattagcacc ttgcttgaat tcctgccctc tgcggatcaa
    14161 gcccataact tcttgatcag taagtgcctc aacggtcaat aaatgcttta agctgatacg
    14221 ttctgatttg atgatcataa tgtttcctcc ttagttttct tcgcttcgtg aagtttctgg
    14281 taagataaga tttaaaatga tgccaagtac agttgctaat gccattgccg acaatgtaaa
    14341 tgtgcctact tcaaagacga gtccgccaat tcctactact aagataactg aagcaatcaa
    14401 cagatttttc tttttatcaa aatcgatttt gttctcgatt aaaatcttca ttccacttgc
    14461 ggcaattact ccaaataaga taaaactgat tcctgagatg actggtccag ggatacttaa
    14521 aatcaaagcg cttaattttc ctacgaatcc taaaatgact gcaaatactg cagctccacc
    14581 gatcacaaac acgctatgga cacgtgtgat agccagaaca ccaatgtttt caccgtaact
    14641 tgttacagga ggacctccga caaaggctgc cacgatttgt gctaatccat cacctgtcaa
    14701 cgtcttatga agacccggtt cttcaaagaa attacgttta gttagtttat tcaataccat
    14761 aagatggccg atatgctctg tcatcgtcac aaaagcgatg ggtgccattg ttgtgattgc
    14821 tccaagatat agctttggtt catattgtac aaagggaatc tcaaagttag gaacagcaaa
    14881 ccaggcagcg tctacgattg gcttagtatc cacgattcca aacagcaagg ctacgatgta
    14941 tccgctaata atacctagta aaatcggaat caatccgaga aaacctctta gccacatgtt
    15001 gaagaaaatt gttaatccta atgtgatcag cgctacagca atatacttga aatcatacgt
    15061 gccctgatta tacatagcat ttgttgctgc atttgccgca agtcccaacc cgataaccat
    15121 gatgactgga ccgacgacga ttggaggcaa gattttatcc agccatgctg aaccgatctt
    15181 tttgatgatc agcgcaacaa tcaaatagac taatccagtt gtgactgctc cttgtgcaat
    15241 cgctggataa ccatctgttt tcatcaaaag ctgcatcgct gcaatgaaag cgaagctgct
    15301 tcctaagtac gcaggtatct ttcctttagt agtaaataag tacaccaatg tacctagtcc
    15361 tgaactgact agcgcaatac ctggatcgat acctactaag atggggacta acacggttgc
    15421 tccgaacatc gtgaacaagt gttgtaaact taatcctacc caatgtcctg gggttgggcg
    15481 atcgtggata tctaacacca cgtcatcatt atgaaatcct tttttctccg acatgtttct
    15541 ctctcctcta atcttcctct ttcaaaatca tgatgcgatc ttgtccatca agctcttgca
    15601 tctctactaa aatctcttcc gcccgagatg ttggaatgtt cttcccaaca taatctgcac
    15661 ggattggcaa ttctcgatgc ccacgatcga ccaggactgc taatgaaatt tttcttggtc
    15721 tgccaaaatc cattactgcg tccattgctg ctcgaatcgt tcgtcctgta tacaaaacat
    15781 catcaatcaa aataacttct tttccttcta aggaaactgg gatgtcagaa gaatgaagtt
    15841 ccggttcttc tggattttct tttttgtcat ctcggtataa agtgatatcc agttctccta
    15901 caggaatatc gatatcttct agctgcttca ggcgttcggc aattcttgat gcaatgtaga
    15961 ttcctcgtgt tttgatacct accaatacga tatcttggat actatggttt ctctcgataa
    16021 tctcataagt aattcgagtc aatgctcgct tcatggttac ctgatctact acttcttttg
    16081 cttgcatttt cattccccct acttttttgc ataaaaaaac tcctgcccta agaggacagg
    16141 aggaatgttt agactgagcg tatactgata cactgatagc tatactatgt caatgagcaa
    16201 tctacttatg gcgcaccata cgtatccttg ctcccaccct tcttagcctc acgggactaa
    16261 acttaaagga attctattaa gttttatgct ttcgttgtta attactatac cgttatctaa
    16321 attaaaatgc aatgtttttt cttaattttt ctaaagtttt ttcaaaaact tctggaagag
    16381 gtgcttcaaa gatcatcttt tcgtttgtag tcgggtgcgt aaaacctaac atttgcgcat
    16441 ggagaaactg accattgcct ttcaaagttt tcttcggtcc gtatactgga tctccagcta
    16501 acggataacc gatatacttc atatgcacac ggatctggtg tgttcttccg gtttctagtt
    16561 ttaattcaac cagagtaaat ccttcgaaac gttccaatac agtaaaatgt gtgacagccg
    16621 acttcccatc ttctcttatc gcctgcattt tccggttttc cttcgaacgg ccgattggcg
    16681 cattgatggt tcctttttcg tgaggaatct ctccatggac taaagcaaca tatttgcgca
    16741 aagaggtctt atcttttaat tgctgagcta atgattcgtg tgcctggtca tttttggcaa
    16801 ccattagcag tccggaagta tctttgtcga ttcggtgaac aatacctgga cgaataacgc
    16861 cgttgatcgt agaaagatcc ttgatatggt acatcagcgc attgaccatt gtgccatgcg
    16921 tatgacctgc tgaaggatga acgaccattc cttgaggttt gtttatcacc gctacatctt
    16981 catcttcgta aacgatcgtc aacgggatat tttctgggac cacatctaat gtttctggtt
    17041 cggggatcaa gacagtaatc tcgtctcctt gttttacttt gtaatttgct ttgactggct
    17101 gtccattgac agaaaccgct ccatttttca gccacagctg aatttgcgaa cgactgtaat
    17161 cggtgaataa attagtcaat actttatcga tcctaccaga ttcatggtga atcgtcgttt
    17221 taagttctgt catttttttc tttgatcctt ttcatctaaa ataatataaa ttaacataca
    17281 aatcactcca catacaagtg tcacatctgc tacgttaaaa atcggaaagc tcataaaatc
    17341 tgtctggaac atatctacta catagccaag acgaatacgg tcgataaaat tgccaatcgc
    17401 accagctaga atcaaactca atccaatcat gaaccagcga ttaccttttt cacgattttt
    17461 aatcatcaat gtgagaatca caacgactgc aatgagtgtc actatagtaa agaaccacat
    17521 cttcccttct agcatactcc atgcggctcc agtgtttcga atatgattca gtgacaaaat
    17581 acctggtata agatttttcg tctctccaag cgctagatta tcagtgatcc accatttgac
    17641 caactgatcc actcctacta gcaaagcgct gaatatccaa taaataatta acaaaaaatc
    17701 aatccttttc tatttgttta taaatatcac gaggtcgtac gatcccgagc agattaccat
    17761 cgggattccc atctttagtg ataagtacgg cttccaaagt cccttgctca cgaaacatcg
    17821 cttcaacctc gtaaatatgt gttttagctg gaacaaaatg ataatttgtg tactttccgt
    17881 tttggatgat caaatccaac gctcgtttat ttgttaaatc aaccggacct ttttggcttt
    17941 cttttgctag ccaaaacccg atattacgta aagtaatcaa tgctacaaat tttcctttat
    18001 tgtataacgg aaattgtgaa tacctttttt ctgctactat ctttaacagt tcaagtaacg
    18061 gaagatcttg ttcaaaacct gttacacgtt ttgcaaatcg aggcagcacg gtttcaggct
    18121 gaatcagttc ttgttcgatc agctggattc gcttgactgt ccaatcattt ggctctgcaa
    18181 tgacaaaatc gactgcgatc cgatcatgga cgatcgcatt ccttaactga gcaagctgta
    18241 aaagatcatc ttcgtatttt ctgatcatta attgttttct ttgagctagg cgtctgacaa
    18301 gttcggtaaa tcccatattt cgtgcatttc ccatctcgtc tctcatccat ttttcgattc
    18361 gattgaaact gattaaaaaa agatcagagt ttgccataat tttcctcctt gatcaaatat
    18421 gcccggtttt ttatatagaa aaagaacgca atcaacacaa taactgcaaa aaaactgaaa
    18481 aataaatttt gtaggaaaac ggcccccaac ccagttgcta gaaataaaaa cggatagatt
    18541 tgaagaatca agtaattcat tgtgatcaca taagcaggct ttgcttcttt cactgtataa
    18601 acggggcttt ttagccaata aaaggtgacc tgcatcgtaa agggcacttc cagatcaagc
    18661 gttatctttt gttcatgact gagaacactg cgtttttcat tgttgattct tagctgaatc
    18721 gagctaccca taccgtaaaa accggttttt cttataaatg tcgttttcat cataccctcc
    18781 tattatgctt tagtatagct caaaatacaa taaagaaaaa gaatgatcct agtgtaaaca
    18841 aaattaattc cctatcgtct aaaaaaagaa gctgtagtca aaatacagct ccttcgatgg
    18901 tagattataa tcaagcttaa aacaatccca acacttctcc gtcatttgtc acgtccatat
    18961 tcaatgcagc aggtttttta ggcaaaccag gcatcgtcat aacatctcca gtcaaggcta
    19021 ctaaaaatcc tgcccctaat tttgggatga tttcgcggat cgtgatggtg aaatcttccg
    19081 gtgcacctag cagtgaagga tcatcagaaa atgaatactg tgtttttgcc atacaaatcg
    19141 gcagtttgtc ccatccattc ttcgtaaact cttctatttg tttttgcgct tttttgctga
    19201 aaacaacacc atttcctccg taaattttct tgacgattgt ttctgtcttt tcttgtatcg
    19261 tttcttctag gcggtataat ggcttgtaat tagcttcttt tctatcaatc aatcgtacta
    19321 cagcttttgc taaatcaacg ccgccttctg ctccatctgc ccatacactc gctctcttgg
    19381 caagaatccc ctgattttca cacaagtgct ccaacagtgt gagttcattg tctgtatctg
    19441 ttacgaattc attgattgcg acgatcacag gtagctgata ttgttccata ttacgtatat
    19501 ggcgctttaa atttgcaaaa cctactttca aagcatcaag attctcattt ttaagttcat
    19561 cttttttcat tccgccatgc attttcaatg cacggacagt ggcgacaatc accacagcat
    19621 ccggcgcttt tttcaagtta ggtactttga tatctaaaaa tttctctccg ccaagatctg
    19681 caccaaatcc tgcctctgtg acaacgtaat ctgctaatct caatgctgtc ttggtagcta
    19741 aaatactatt gcagccatga gcgatattgg caaacggtcc cccgtggaca aaagctggtg
    19801 ttccgtaaat tgtttgtaca agattcggct tgattgcatc tttcagcagt aatgccagag
    19861 cgccttctgc tttcaagtct ccagcagtca caggctgacg gtcaaatgta tagccgacaa
    19921 cgatccttgc aagacgagcc tttaaatctt caaggtctgc cgcaagacac aagatagcca
    19981 tgatttcgct ggcaactgtg atatcaaacc catcttctct tggaacacct tggattggcc
    20041 cacccaagcc aacaattact ttacgcagtt cacgatcatt gagatcgact acccgtttcc
    20101 aaatcactcg gcgggcatcg atgttcaatt catttccttg atgaatatga ttatctaaaa
    20161 gagcagataa tgcattatta gctgttgtga tagcatgcat gtcaccagta aaatgcaagt
    20221 tgatatcttc cataggcaat acctgtgcat atcctccgcc tgcagcacca cctttgattc
    20281 ccatcacagg accaagtgag ggttctcgca atgcaataac agatttttta ttgattttat
    20341 tgagcgcgtc tcctaaccct acagtgatcg ttgactttcc ttcgccagct ggtgttggat
    20401 tgatggaagt aactaagatc aaatgcccct cagcgttatc ctttgatcga ttgattccag
    20461 aaaaattgat tttcgcctta tatttcccat acagttccaa atcgtcttcc ttaagatcta
    20521 atttactagc cacttcaaca atcggttcta gctctgtttc ttgtgaaatt tgcagatcat
    20581 tcttcatttg attacccctt ttcacgaact ttattcaaca aaatatcttc tttgttaaga
    20641 tatgccaatt ataccttttt ttattcagaa aaaaaacata aaattacagg
//
LOCUS       DAJSMI010000034        20441 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJSMI010000034 DAJSMI010000000
VERSION     DAJSMI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.034"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            214..498
                     /locus_tag="ON430_001015"
     CDS             214..498
                     /locus_tag="ON430_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCR3218943.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="ON430_001016"
     CDS             complement(779..1198)
                     /locus_tag="ON430_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCR3218944.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="ON430_001017"
     CDS             complement(1200..1784)
                     /locus_tag="ON430_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR3218945.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ON430_001018"
     CDS             complement(1831..3306)
                     /locus_tag="ON430_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCR3218946.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ON430_001019"
     CDS             3487..3798
                     /locus_tag="ON430_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218947.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="ON430_001020"
     CDS             complement(3913..6249)
                     /locus_tag="ON430_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3218948.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="ON430_001021"
     CDS             6391..7059
                     /locus_tag="ON430_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3218949.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="ON430_001022"
     CDS             7062..8105
                     /locus_tag="ON430_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3218950.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="ON430_001023"
     CDS             complement(8342..9223)
                     /locus_tag="ON430_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3218951.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="ON430_001024"
     CDS             9382..10110
                     /locus_tag="ON430_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR3218952.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="ON430_001025"
     CDS             10266..10583
                     /locus_tag="ON430_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3218953.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="ON430_001026"
     CDS             10576..11184
                     /locus_tag="ON430_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCR3218954.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="ON430_001027"
     CDS             11239..12030
                     /locus_tag="ON430_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCR3218955.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="ON430_001028"
     CDS             12168..12656
                     /locus_tag="ON430_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCR3218956.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="ON430_001029"
     CDS             12707..13579
                     /locus_tag="ON430_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR3218957.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="ON430_001030"
     CDS             13755..14117
                     /locus_tag="ON430_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3218958.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="ON430_001031"
     CDS             14126..14878
                     /locus_tag="ON430_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3218959.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIDQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="ON430_001032"
     CDS             14892..16694
                     /locus_tag="ON430_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3218960.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="ON430_001033"
     CDS             16971..18011
                     /locus_tag="ON430_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3218961.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="ON430_001034"
     CDS             complement(18063..20207)
                     /locus_tag="ON430_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3218962.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         6263 a         3586 c         4232 g         6360 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccatat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatcgatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatgc tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt t
//
LOCUS       DAJSMI010000035        20241 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJSMI010000035 DAJSMI010000000
VERSION     DAJSMI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.035"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(37..1596)
                     /locus_tag="ON430_001035"
     CDS             complement(37..1596)
                     /locus_tag="ON430_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCR3218963.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1751..3985
                     /gene="pcrA"
                     /locus_tag="ON430_001036"
     CDS             1751..3985
                     /gene="pcrA"
                     /locus_tag="ON430_001036"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCR3218964.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4002..6038
                     /gene="ligA"
                     /locus_tag="ON430_001037"
     CDS             4002..6038
                     /gene="ligA"
                     /locus_tag="ON430_001037"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR3218965.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6144..6449
                     /gene="gatC"
                     /locus_tag="ON430_001038"
     CDS             6144..6449
                     /gene="gatC"
                     /locus_tag="ON430_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCR3218966.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6449..7921
                     /gene="gatA"
                     /locus_tag="ON430_001039"
     CDS             6449..7921
                     /gene="gatA"
                     /locus_tag="ON430_001039"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCR3218967.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7918..9348
                     /gene="gatB"
                     /locus_tag="ON430_001040"
     CDS             7918..9348
                     /gene="gatB"
                     /locus_tag="ON430_001040"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCR3218968.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9358..10413
                     /locus_tag="ON430_001041"
     CDS             9358..10413
                     /locus_tag="ON430_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR3218969.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10524..11897
                     /gene="rlmD"
                     /locus_tag="ON430_001042"
     CDS             10524..11897
                     /gene="rlmD"
                     /locus_tag="ON430_001042"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR3218970.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11900..12028
                     /locus_tag="ON430_001043"
     CDS             11900..12028
                     /locus_tag="ON430_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR3218971.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12468..12656
                     /locus_tag="ON430_001044"
     CDS             12468..12656
                     /locus_tag="ON430_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR3218972.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12734..13747
                     /locus_tag="ON430_001045"
     CDS             12734..13747
                     /locus_tag="ON430_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3218973.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13814..14848)
                     /locus_tag="ON430_001046"
     CDS             complement(13814..14848)
                     /locus_tag="ON430_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3218974.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            14963..17188
                     /locus_tag="ON430_001047"
     CDS             14963..17188
                     /locus_tag="ON430_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR3218975.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17206..19089
                     /locus_tag="ON430_001048"
     CDS             17206..19089
                     /locus_tag="ON430_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCR3218976.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19070..20044
                     /locus_tag="ON430_001049"
     CDS             19070..20044
                     /locus_tag="ON430_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCR3218977.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6826 a         3711 c         4338 g         5366 t
ORIGIN      
        1 atgaatagtt gttgtaaaat agtttttcca tgttcgttaa aatgattgct tcaaatttgt
       61 gatagaagat ttagcctcga tttcctgctt gattcttggc aagtttgctt catcagtcaa
      121 gataaccaaa atatctccta cattcatgat cgtatcaccg tgagtcaaga tttcctgatt
      181 tcctctgcgt atagagatta gcagcatatt tttaggccaa ttgaagtctc gtaccattaa
      241 tccgtctaaa gaactctctg ccgttactgg aaaatcaaaa gttgtccttg caccttttag
      301 tgaggcggtc tgagtatttc caagtagacg ttccaataat gattcgtaaa ttggattacc
      361 gccgagaagg tcattgacga tataagcaac aagagcagcc aatcctaaag gcatcagatg
      421 attaagagag ccaaccattt ctgtcactaa aataattgct gtcagcggtg cttttccaat
      481 cgctgtgaaa taaccagcca tggcaataac gacaaaatcc ttgatgtaga ttggatccat
      541 cccgaaatca accataatgt ttccataaaa gctaccgatt aaagccccca atgtcaatat
      601 cggaaggaaa atcccccctg gaagaccccc accgtaagaa atcatggaaa aaacgaatcg
      661 aagaacaaac aaaccgataa tcataatgat tggcaaggaa gcatgtccaa aagctaaaac
      721 gatttgattg cctccgccta gagtactagg ccataaataa ccaattggca aaatcagcaa
      781 gaaaggaacg aacccgtgaa aataagctgg taaaggcaat cggttgtaaa attttggcaa
      841 agccagcaag acattctgat aaataaaacc taagatgcct agaagtactc ctaagatcac
      901 taaggaacca taatatttca acggcaaaga aggaaggtca cctagatata aaacaggtct
      961 caagccgaaa aagtaaagag agacgaagtt agcagtgatt gctgaagcaa atgatgtcag
     1021 ccaaacaagt ggagaaaagg agtgatggat ttcttctaaa acaaagagta atcctgcgat
     1081 aggagcattg aatgctgctg caagtccgct acttgcaccg ctagaaatta aaatcttttc
     1141 ttctgaagat ggactccgaa acaattgtcc agctccttgt ccaatggatg cgccaagctg
     1201 tatagaaggt ccttctctac ctagaaaaag gcccgcacca actgaaagta caccgccaat
     1261 gaactttttc cataaaacag agaaccagtt cattgataat tccccgcgaa gctgtccctc
     1321 tacttgtgga atcccgcttc ccttgatatc cggttcattt ttgaccagat aacccacgaa
     1381 aaatgctgca aggatgcagc aaatagctag gccaattaag taaatcggct gttcccgact
     1441 catttcatag atacttcgaa ctaaatcgct tagcttttcg atagataatc gaaacaggct
     1501 aacgacgatt cctgtcagca gtccaacaaa tattcctttt agaataaaat atatctgagt
     1561 cttatctaat ttcttgattt ttcctttttt ttccattcaa acgcctcgct ttcttttaat
     1621 ttagcgcgaa atcaacgaat tgacaaggaa aaatccataa aaagaaacaa atgattttga
     1681 actagctagt ttaatgaaag ccaagaaaat gctataatta acaagattga taaaagaaag
     1741 gatgttgtta atgtcatcca aagctgaatt attaaacggg atgaaccccc gtcagaaaga
     1801 agctgttctt cacactgacg gaccattact gctaatggca ggtgcaggaa gtggaaagac
     1861 gagagtgctg acacaccgca ttgcctattt gattgaggaa aaagaagtca atccttggaa
     1921 catcctagcg attactttta ccaataaggc tgcaaaagaa atgaaagaac gtgtcaatgc
     1981 gattctcgca tctggaggcg aagatgtctg ggtatcgact tttcactcga tgtgtgtgcg
     2041 tattttaaga agagatgttg attttatcgg gtataaccgg aactttacaa tcatcgattc
     2101 ttctgaacag ctgaccttga tgaaacggat actaaaagaa ctgaacattg atccgaaaaa
     2161 atatgatcct cgaagtatat taggaacaat ctctcaagca aaaaacagcc tgcagacccc
     2221 tcaggatttt gcaaaaatgc aaggcagtta ttacgaagag attgcggcaa aatgttatgc
     2281 tgcttaccaa aaagagcttc aatataatca atgtatggac tttgacgacc tgatcatgaa
     2341 tacgattcga ttatttgaag aacatcctga ttcactcacg tattatcaaa acaaattcca
     2401 ttatattcat gtagatgagt accaagatac aaatcacgca cagtatacgc tagtcaattt
     2461 attggcaggt agatttagga acctgtgcgt cgtgggagat gcagatcaaa gtatttacgg
     2521 ctggcgcggt gcagatatgc agaacatctt ggattttgaa aaagattatc cagatgctgc
     2581 agtaattttg ctagaacaaa actatcgctc cacgaaaaac atccttagcg cagcaaacca
     2641 agtcattgaa aacaatagca atcgaaaacc aaaaaacttg tggacagaaa acaaagaagg
     2701 aaacaagatc acttactacc gtgcagataa tgaacgagac gaaacccgtt ttatcgtaga
     2761 ccggatgcaa gaagagattc gaagcaatca tcggaattac ggcgactttg ccatcttgta
     2821 ccggacgaac gcacaatctc gtgtaatgga agaaactctg ttgaaagcaa atattcctta
     2881 taaaatggtt ggagggcaca agttctacga tcgtaaagaa atcaaagata ttttagcgta
     2941 tttgaatgtg ctggtaaatc ctcaagattc aatcagtttt gagcgtatcg ttaattcgcc
     3001 caaacgaggc atcggaccag gatctatcga aaaactacgc agttttgctt ctcttcatga
     3061 atggccactg cttgaagcag cgcaaaatgt ggatctggca aatatcggtg gaaaagcagg
     3121 acagcaatta ggtgcatttg gagagatgat ccaagaagtc actcagatga tcccatactt
     3181 gacagtgaca gaattgacga aagaggtttt ggatcgtagc ggatatctag aagacttaaa
     3241 aatccaaaat acattagaag cacaagcacg catcgaaaac ttggaggaat ttttaacggt
     3301 cacacaagaa ttcgacaaac aattcgaaca gcaaaatgaa gaagacgctg atgcaccaga
     3361 agaaaaacta acggtcttct taaatgatct ggcacttgtt tccgacattg acaatttaga
     3421 agaagatgct tcccaagtga cattgatgac tttgcatgca gccaaaggat tagaatttcc
     3481 agttgtattc ctgattggtc tagaagaggg ggtttttcct ttatctcgag cattgatgga
     3541 agaaagcgaa ctggaggaag aacgccgtct cgcatatgtg gggatcacgc gagcagaaga
     3601 agcgttgtat ttaacaaatg ctttctccag aacattatat ggacggacgc aatataaccg
     3661 accttctcgt tttgtagaag aaatcgatca ggagttactg gaaatcgaag gcatgcgtcc
     3721 tacgcctaaa aaaacgcctg tattcgctaa aaaaacagca tatagctata aacagccaga
     3781 gacagcggtt gttccctcta aaagtgctac tggaggggaa aataacagct ggaaacctgg
     3841 agataaagtc aaacacaaaa aatggggtct aggaacagtc gtccgagtaa gcgggacgtc
     3901 gaaggatcta gaactagatg tggctttccc aagtcaagga gtaaaacgtc ttttggctgc
     3961 ctttgcgcca attgaaaaag cttagtagtg gagggtaaat gatggaacat gaaatgacga
     4021 ttgacgaagc tgctaaacga gcagaagaac ttcgtacaag actgaaccaa tggtctcgcg
     4081 aatactacgt agaagataaa ccaacagtgg aagactatgt ttatgataaa gaatacgcag
     4141 aattagtagc aatcgaggaa caatatcctg atttgatcac gtctgattct cccactcaac
     4201 gagtaggcgg aaaagtatta gaaggttttg aaaaagtcac tcatgatatt ccgctttaca
     4261 gtttgaacga cgttttcagc aaagaagagc tgatagcttt tgatcaacgt gtacaaaaag
     4321 cagtgggacg tgtagttgac tattgctgtg aactaaaaat agatggactt tccgtttctt
     4381 tgcgttatga agatggaaat ttcgtgagag gtgcgactcg cggggatgga actgtaggag
     4441 aaaacatcac tgaaaaccta aaaactgtcc ggtcggtccc aatcaaatta aaagaaccaa
     4501 tgaacatcga agtaagaggc gaatgtttca tgccaaaacg gtcatttgtc caattgaacc
     4561 aagacagaga agcagaggga aaagatatct ttgcgaatcc tcggaacgcc gcagctggtt
     4621 ctttgcgtca gttagattct aaaatcacag cgaaaagaaa tctggacact tttttataca
     4681 cagtagctga tttcggtcct atgcaagcaa aaacccagta tgatgcgcta gaagagctag
     4741 aaaaaattgg attccacacg aatcgggaaa aacgtctatg tcattcgatt gatgaagtgt
     4801 ggtcatacat tgaagaatat catgacaaac gtgtggattt gccctatgaa atcgatggaa
     4861 tcgtcatcaa agtgaatgaa ttctcgctac aagatcaact tggttttact atcaaagcac
     4921 cgagatgggc agcagcttac aagtttccgc cagaagaagt tgaaacgcta atcgaaaaca
     4981 tcgaatggac agtcggtcga acaggggtag taacgccaac agctatcatg acaccagtcc
     5041 gagtagcagg aacgacagta agccgtgcca gcctgcataa cggtgattat attaagttga
     5101 aggatatccg cttgaaagat acagttctga tttataaagc gggagacatc atcccagaag
     5161 tttcgcaagt cgtgttagat aaacgaccta aagacagcga agaataccaa ttgcctacac
     5221 attgtccagt ttgtggaagt gaacttgtcc atttggatga agaagtagcc ttacggtgca
     5281 tcaatcctaa atgccctgct caaatgaaag aaggtttgaa ccactttgtt tcaagaaatg
     5341 cgatgaatat cgatgggttg gggccgcgcg tactagaaca gatgtatgat aaaaaactag
     5401 tagcagatgt tgctgatctt tacaaattga cagaagaaga gttattgacg ttggacaaaa
     5461 taaaagaaaa atcagcaaat aatattttga cagcaatcga caacagcaaa gataattcgg
     5521 tcgagcggtt gattttcggg ttaggaatcc gacatgtcgg agcgaaagca gcaaaaattt
     5581 tagcagaaca tttcggtgat ctagaaacat tgagtaaaag tgactatgag tcgattattg
     5641 cgcttgatac gatcggtgat atcattgccg acagtgtagt cacttatttc agtaacgaag
     5701 aagtccatga attgatgaat gaattaaaac aagcaggcgt caatttcgaa tacaaagggc
     5761 ttcgaaatgc ccaattgcaa gaagtcgagt ctccttttaa agaaaaaaca gtagtcctta
     5821 ctggtaaact aacacgattt actcgtgaag aagcaaaaga gacgatcgaa aatcttggcg
     5881 ggaaagtgac tggtagcgta tcgaaaaaaa cggatatcgt ggtagcagga gaagatgctg
     5941 gcagcaagct gaccaaagca caagaattag gaatcgaagt ttggacagaa gatcaaatgg
     6001 cagatgcatt ggctaaaagt cgctcagtag aagaatgaaa aatgagtttt ttcagacaaa
     6061 tttcaataaa caatttcaga cacggcactc ttgtgttaaa atagagagca aagtttttaa
     6121 ctcgtttaga aagaaggggt agtatggcga ttagtgaaga acaagtgaaa cacgttgcca
     6181 aattagcaaa acttgcgttc gcagatgaag aacttgcaag ttttaccgac caattaggta
     6241 aaatcatcga tatggttgaa atgctggaag aagtagatac ggaaggtgtt ccatttacgt
     6301 cgaatgtggt tgaaacagta aacgtgatgc gacaagatcg ggcgcaagct ggatggaacc
     6361 gcgatgaatt attgaaaaat gtgccggaaa cagaagacgg ctttatcaaa gtacccgcaa
     6421 tcatcgataa tggggaggct ggcgcataat gacagaatta cacaatcgtt ctctagaaga
     6481 actgcatcat atgcttgtat cgaaagaaat cactgtacaa gatttgacaa aaacaacatt
     6541 tgaacgaatc aaagaaacag agcctgaggt cgaggcattc attacattga acgaagaaaa
     6601 agcattggaa caggcaaaag ctttagatga aaaagggatt gatgaaagca acgtccttgc
     6661 tggtattcca attggtatca aggataatat cgtaacaaaa gatatcttga caaccgctgc
     6721 atctaagatc ctttacaatt ttgaaccaat ttatgatgca acggtcatgg ataaaatcta
     6781 tcaagcagat atgatccctg ttggtaagct aaacatggat gaatttgcta tgggtggaag
     6841 tacagaaact tcttatttca agaaaacaaa aaatgcttgg gacccaacaa aagtacctgg
     6901 tggctcttct ggtggttctg cagcagcagt tgcagctgga caaattccag tttctttagg
     6961 tagtgatact gggggaagta tccgccaacc agcagctttt aacggcatcg tagggatgaa
     7021 acctacttat ggccgtgtct cacgttttgg tttgattgcc tttgcttcat ctcttgacca
     7081 aatcggacca atgacccgta cggtgaaaga taatgcatta gcattgacag ctatcagcgg
     7141 ttatgacgaa aaagacggta cttcttcagg cgtttcagtt cctgattttg cagagggact
     7201 gacaggcgac atcaaaggaa tgaagattgc tttacctaaa gaatacttag gcgaaggtgt
     7261 ggcccctggc gtaaaagaag ctgtattgaa agcagcagaa acattccgtt cattaggtgc
     7321 tgttgtggaa gaagtcagct tgcctcattc aaaatacggt gtggctgttt attatatcat
     7381 tgcctcatca gaagcaagtt ccaatttgca acgattcgat ggtatccgct acggttatcg
     7441 ctctgaaaat gtgaaatcat tagatgatgt atatgtcaac tcacgttcag aaggattcgg
     7501 tgaagaagta aaacgccgta tcatgttagg tacattctcc ttaagcgcgg gttattatga
     7561 cgcttacttc aaaaaagcag gacaagtccg tacattgatc aaacgagatt ttgaaaaagt
     7621 ctttgcagat tatgacttga tcatcgggcc atcttcacca acagttgcat tcggaatcgg
     7681 tgagaacatt aacgatccaa tcacaatgta catgagtgac atcttaacga tccctgtcaa
     7741 cttggctgga cttccaggta tgtctgtacc ggcaggttta tcagaaggat taccagttgg
     7801 attgcagatc atcggtaatt attttgatga aaaaacgatg tatcaagcgg catatgcgtt
     7861 tgaacaagca acagaattcc ataaacaaca accagctatt ttaggaggga aagacgaatg
     7921 aattttgaaa cagtcattgg actagaagtc cacgtcgagt taaaaaccaa ctcaaaaatt
     7981 ttctctccag cgccagctca ttttggtgca gaaccaaaca gcaatacaaa cgtgatcgac
     8041 tggggttatc caggcgtgct gccagtaatg aataaagctg cgctggaatt cgggatgaaa
     8101 gcagctttgg cattaaactg tgaaatctct aaagatactc attttgaccg gaaaaattac
     8161 ttttacccag ataatccaaa agcatatcaa atttcccaat ttgatcaacc aatcggtcat
     8221 gacggatgga tcgaaatcga agtagaaggt aaaaagaaaa aaatccggat cgaacgtgtc
     8281 catttagaag aagatgctgg aaaaaacatg catggtattg gcggttattc ttacgttgat
     8341 ttgaaccgac aaggcacgcc tttgatcgaa atcgtttctg aagctgatat gcgttcacca
     8401 gaagaagcat acgcttatct agaagcattg cgatcaatta tccaatttac agaagtcagc
     8461 gatgtgaaaa tggaagaagg ctctatgcgc tgtgatgcca atatttctct tcgcccttat
     8521 ggacaagaag agttcggtac gaaagcagaa ttgaaaaact tgaactcttt atctttcgtg
     8581 aaaaaaggtt tagcttttga agaaaaacgt caagcaaaag ttcttctttc aggaggagaa
     8641 atccaacaag aaacacgccg ttttgacgaa acaacgaata aaacagtttt gatgcgtgtc
     8701 aaagaagggt ctagtgatta tcgttacttc ccagagcctg atatcccgaa attcgtgatc
     8761 gatgacgaat ggattcaaaa agtaaaagct agtttgcctg agatgccagc ttctcgtcgc
     8821 gaacgctata tccgtgagtt tggacttcca gagtatgacg caatggtatt gacacttaca
     8881 aaagaaatgt ctgatttttt tgaagctgtt ttggaaaatg gtgcagatgc caagcaagca
     8941 tctaactggt tgatgggtga agtttctgct tacttgaaca gtgagaaact ggaattagct
     9001 gagacaaaat tgacacctga gaacttagca ggaatgattc gtttgatcgc cgatggaacg
     9061 atcagctcaa aaatcgccaa aaaagtcttc cgtgaattga tcacaaatgg cggagaagcc
     9121 caagaagtag ttgaaaaaaa tggttgggtg caattatctg atccgaacaa actactgcca
     9181 atcattaatg aaattttgga taacaaccaa caatctgtcg atgatttcaa aaatggaaaa
     9241 gatcgtgcgg ttggcttcct agtcggtcaa atcatgaaag caactaaagg acaagcaaat
     9301 cctggtgtcg tgaataagct acttaaagat gaattagcga aacgatagag gctgaaaatg
     9361 aaaaaagcac gcgtaattta taaccccacc tcgggaaaag aattattgaa aaaaaatttg
     9421 gcggatatcc tacaggcatt agaagaagca ggatttgaag ccagcgccta tgcaactaca
     9481 ccagaaccag attcagccaa gaatgaagca tgtagagtcg cagaactagg ttttgatcta
     9541 gtagtggcag ctggtggcga tggcacaatc aacgaagtgg ttaatggaat cgctccgctt
     9601 gaacaccgtc ctaaaatggc catcatccca gctggtacaa cgaacgacta tgctcgagca
     9661 ttaaaaattc ctagagataa tattaaagca gcagcagaag tgataaaaaa acaccaaacc
     9721 gtaaaaatgg acatcggcct ttctgataac agttacttca tcaatatcgc tgcaggtggc
     9781 tatttgacag aattgactta tgaagttcca tcagaactaa agagtatttt tggttatctt
     9841 gcttatcttg cgaaaggagc agagatgctc cctcgagtaa agccaatcaa aatgcgcttg
     9901 aagtatgatg aaggggaata tatcggaaat gcttcgatgt ttttcctagg gctgacgaac
     9961 tctgttggag gattcgaaca gatcgcgcca gatgccaaat tggatgatgg gaagttttct
    10021 ttaatcattg tgaagacagc aaatatcttt gagatcttgc atattgcagc actcatgctg
    10081 aatggcggtc gacatgtcga tgatccaaga ataatctata ccaaaacaag cagtttgttc
    10141 gttgaaacag aagatgatat caatcgacca gtaatgatca acttagatgg tgaatacggc
    10201 ggggaagcac cgatgcattt ccaaaatctt catcaacaca ttgagttctt tgccaatacc
    10261 gatcagattc ctgatgaagc aatcacaggt acagatgaag aagaattaga ggttgtcagc
    10321 aaagaattcg taaaagaagt cgaacgttta acggatgaag atatcgacgg tgacggaaaa
    10381 atagcagacg aaacacctaa tcaaaagaat taatcaaaag ccggtgctga acgtagtaag
    10441 gtcagtgccg gttttagttg tgtaattagc tgatttatag tagagttcag taggattgaa
    10501 aaataggctt aaaggagcaa aatatgacag aacagcaaat agtaaagaaa aacgaacgat
    10561 atacagtcga aatcgtcgac ttgagttatg aaggaatggg ggtagcaaaa atcgatggtt
    10621 atccattatt tatcgaaaat gcactccctg gagaagaagt agaaatacta gtagtaaaag
    10681 caggcaataa gtttggatat ggaaaagtcg aaacatttat tacacaatca cctgatcgtc
    10741 agcctgtaga aaacaatgca ttacttcgca taggtatcgc acctttagcc catttgaagt
    10801 atgaacaaca gcttgtcttc aaacaaaaac aagtagagca tgtaatggcg aagattgcta
    10861 agatgccaga agttcctgta cttccaacgg tcggaatggc agaaccattc ggctaccgta
    10921 ataaagcaca gatccctgtt cgccgtatcg atggacagtt agccaccggt ttttacaaaa
    10981 agaatagcca tgatctagtc cctatcgaag atttttatat ccaagatcct gtcatcgatc
    11041 aagcaatcaa agtagtcaga gatatcttgc aacggttcca agtacgagga tataatgaag
    11101 aaaaaaatga aggacagctt cgtcacatca ttatccgtcg tggtcattat tcccatgaaa
    11161 tgatggtcgt tttagttact cgtaaagaaa aattctttaa atccaaggaa attgctgaag
    11221 ctattcatga agaattgcca gaagtggttt cggtcatcca aaatatcaat gaagaaaaaa
    11281 caaatgtgat tttaggagac aaagaaaaag ttttatatgg cagagactat atcgaagatc
    11341 agctattagg aaaaacctat cgaatctcag caaagtcatt ttatcaagta aatactcaac
    11401 aagcagagta tctgtaccaa acagccatcg agtttgccga gttagataaa aaagacattg
    11461 tgatcgacgc ttattcagga ataggtacga ttggcttgtc tttagcagac agagtggata
    11521 aggtgtatgg gatggaagtt attccagaag caattgaaga tgcacagttc aatgctttaa
    11581 ataataaaat cgaaaatgtt cattatgaag tcggaaaagc agaaaaagtt atgaaaaaat
    11641 ggcaagaaaa agggatcaaa ccatctgtga tcgtagttga ccctccacga aaaggattag
    11701 atcatcattt tattgaatca gcaatcgaaa tgtcaccaga acgaatcgtc tatatctcat
    11761 gtaatccggc aacttttgca agagatgcta agttatttgc agaagcaggt tatgccgtga
    11821 agaaagttca gcctgtagat cttttcccgc aaacccatca tatcgaatta gtcgcttcat
    11881 tcgttaaagg agaataaaaa tggaccaaaa agaaaaaatg aaagaattga tcaaaaagaa
    11941 acaaggtgga ggccgatcca aacaaatgga aacacctaag aatgatcgta aaaacatgag
    12001 aaaaggccct aaaatcttta ataaatagac gtgctaaaga aagactaaga agtgttaact
    12061 tttgttattg tttcttagtc tttttagtta gtctacatta ttttggcgat ttgccaataa
    12121 ggactgttga gttgtgcaaa atcaaatctg ggtcagaatg aacgtcattc tgtctcagat
    12181 ttttcgttat ttcaatttga ttaactgatt gatcaaaaag atttttattt tcatattctc
    12241 aaatgattca acactaaata tacatgtttg acgcggaaat tattattcga ttttggcggc
    12301 atcagggggg atgacgacgt agcagaccca ttattttact caagaaaatg tccgtgccta
    12361 ttatttagaa tacgatagtg aacgtgctgg gagttttgtt cttttagaga aagtctctga
    12421 agataaatta gttgtcttag attttgactg taacgaaaaa tggcgaaatg gaagagcacc
    12481 agcaagtgat tgaccggatc tacgaagcga cacgatatct gcctttggat cgtttgtgct
    12541 taagtcctca atgtggattt gcttcaacag aagaagaaaa tgttttgttt gaagaacaat
    12601 ggaaaaaatt tcttttgtca aaagcattgc agaagaagtt tggggaaaat cattaaatca
    12661 tctaggagag cttgatagga aataacacta tggtataatc agaatgctta atactaacta
    12721 ctgagggagc cttatgaaaa agaaaattct taaatttatc ggatggttct tcggaatcat
    12781 tcttgggatc gcgtttgcaa tctttcttgc cttccagctt tcccccagac ctggagcatt
    12841 cttgatcaac catatgtttt caaatactgt tcaaatcact gataaaaaaa cgttcgatca
    12901 agcagaaaaa aacgtggcga aaaaaacaga tttgacgtat gagtccaagt ataaagagaa
    12961 tacttatgac atctactatc caaaaaacag caaggggcct gttcctgttc tattttgggt
    13021 acatggtgga ggattcgtcg gcggagataa atcgggtgtc aaagaatttg cgacaaaatt
    13081 agtttcagac gccaacatcg cagtggttgc gatgaattat gaattagcgc ctgattcaga
    13141 ataccctaat caagttcttc aagtgaatga actgatcaaa gatttattag ctgaagaaaa
    13201 agacgacaaa ttgctagaca tggaacaatt attttttggt ggagatagtg caggttcaca
    13261 aatcgcattg caatatgcag ctatacaaac gaatgatgcg tatgctaaac aaatgaaaat
    13321 caaacagcta cttccaaaag atagtctgaa aggaaccatt tcttattgcg gacctgttga
    13381 tttgaagcta acagcacagc agcattcgga tgatcgattt atgaagtttt tcgtcaaaac
    13441 cgttgcgtgg tcgttgattg gaacaaaaga ttggaaaacg agtgagcaat tgaaagaagc
    13501 tagtgtggct gaccatgttt ctaaggaatt tccgccaacg tatataactg acggaaatgc
    13561 atactcattc caggagcaag gtcaagcatt agaaaatcgg ttgacagaat taaaggtacc
    13621 cgtgcaatcc ttgttttata cagatacaaa aaaagagatt acccatgaat accaatttga
    13681 ttacacgcta aaagaatcgc aaaattgtta ccagcagaca ttggattttg tgaataagta
    13741 taagtaagag gttgtgaaaa agccgtttcg ttttgagtat gaagaaacga agcggctttt
    13801 cacaacctct ctttcaatac ctttttgttc ttgggataaa actttcccta acaatcaatc
    13861 gtgcaggata agtcaaaatg attggttcgt cccgtatacc gtcaattcgc tccaatgcca
    13921 ttcgaacagc tgcttgccca attgatttcg cattgatttg aacactggtc aagccagggg
    13981 agaggtttgc gattgtctcg atattatcaa agcttgctac aacaatctct tctccaattt
    14041 ggatattttt ttgtttcaat gcccgataga caccaattgc cattggatcg ctcccgacaa
    14101 gaatggcagt aggaagaggg gattcatttt ctaatagatt agtcgcaagt acctcacctt
    14161 gtttttctgt ccagccacct agatgataag acatatattt ttctaagtga tgatcgatca
    14221 taaaatgctg ataggcacgc atcctctttt catttataga aatctgcttc ccaccgaatt
    14281 catccatatc gactttatat cccccgatat aggctattct tttatgacca ttttctaaga
    14341 acaattccag tacggtttta gtcatccgtt caagatctgc atacaccata tcgacctctt
    14401 gctggatgtc agggttatcc acgacgacta tttgcatgtt ttgttttaac aaggcggtaa
    14461 tcgattcttt cgtaacagtc ccaacaacga tcaccgcacc taactgatct agatcttccc
    14521 attgtttagg attatcagat agattataga ttcgattcat ccctaaatgc agacgtcgag
    14581 cttcttcttc catccctact cgcaaatgtt tgtaatagat atcctgttct tcgctcgctt
    14641 tgccgatcgt agagatcaca ccaatcgaag gcattctctt tttgatatat ttacgagtac
    14701 tgatgtcata atccaacgca gcagctgcct ctactatccg ttgccgagta gtctgagcca
    14761 catggatcgt ctgatcgtta ttcaacacac gggaaactgt agcaggagag acatttgcct
    14821 ttcgtgcgac atctctaatt ccaaccattt ttacacactc ctcaaaaaaa taaaaaattt
    14881 ttattgacaa aattgtttag taaacagtat acaggaatta taaaaaaaag taaacggttt
    14941 ccgttttgtt gggagtgaaa tgatggatat tacgcataga aaaacagata aaaatttcat
    15001 cttatttgat gaaaagagcc gagtcttcca tttacgaaat acatttcttt cttatctgat
    15061 aaagatagaa gaaagcaatg tattggcgca tatttatttt ggcaaaccag tcaagcagta
    15121 caaagacaat aaaaattatc cccgaagaga tcgtggattt tcaggaaatg ttcccttaaa
    15181 tcctgaccgg tctctgtcga aagatacact accgcaagaa tactcttctc atggcagcat
    15241 ggactttcga acgcccgcat cgatcattca gcgcaagaat ggatcagact tactggatct
    15301 gagatatgac agccattata ttacagatgg gaagccagag atcgaaggct tgcctcaaac
    15361 ttatgtgatg gacaagtcag aggcacaaac gttggtgatt tcacttaagg atagagaaac
    15421 agcgatttat ttcgatttgt tctataccat atttactgat agagcagtga tcacacgatc
    15481 tgtaaagata agaaatgaaa ccggagaaac aatcaaacta gaaaaagcgg ctagtttcca
    15541 gctagacttt gcccatacta ggcgatttga tgaagtcatt gctttgccag gagcacatgt
    15601 caatgaaaga cagatttcac ggcaatctgt tttatctgga acaaaagtat ttgaaagccg
    15661 tcgaggaacg agcagtcacc atatgaataa ctttattgct ttagttcatc atcatacgac
    15721 agaaaatact ggagaagcga tcggacttca atttgtttat tccggcaacc attctttcga
    15781 acttgaaaaa gatcagatca atcaattgag agtcgtcggt gggatcaaca gccatcgttt
    15841 ttcgtgggaa ttgaatgcag gccagtcttt tcaaacacct gaaatgatcc tttcttactc
    15901 aagtcaggga ttgaacaaaa tgagtcagat ccaccatgag ttgctacgtg aaagaatcgc
    15961 aagaggtcgt catcaatttg ctgaacggcc gattctggtc aataactggg aagccactta
    16021 ctttgacttc aatagtgaaa aaatcaaggc gatcattgat gaagcaaagg agctaggcat
    16081 cgaaatgttc gtattagatg atggctggtt tggaaaaaga gatgcggaca attcttcatt
    16141 aggagactgg ttcgagtatg aaggcaagtt gacaaatgga ttacgtgaaa tagcagatta
    16201 tgctcatagc aaaggattga aatttggttt atggtttgaa cctgaaatga tctcggttga
    16261 ttctgaactg tatcgaacac atcctgattt tcttatgcag gagccaggta gaatgccttc
    16321 tgcttccaga tcacaacatg tactggattt tacaaggtta gatgttcggc agacgattga
    16381 gaaacaaatg agaaagattt tagatacgat tcctcttgac tacatcaaat gggatatgaa
    16441 ccgttcgctt tcagatgtct attcgattac ccttgaccca cagagacaag gagaagtagc
    16501 gcatcgttat atgctcgggc tttatgaact acttgaacat ttgacaactg attatccgga
    16561 aatcttatgg gaaggctgtt ctggtggagg tggacggttt gatgccgggt ttatttatta
    16621 tatgccgcag tcatggacaa gtgataacac ggatgcagtg gagcgtatga aaatccaata
    16681 tggaacaagc cttgcttatc cgatttcgtc catcacagct catgtatcag ctgtaccaaa
    16741 tcatcagacc gacagatcta cttcgttgaa aacaagagga gaaacagcta tgagcgctgt
    16801 ttttggttat gaactggatt tgacaaagct gtctcctgaa gaaaaaaagc aagtaaaaga
    16861 ccaaattata tcctatcaaa cgattcgtcc ggtgatccag tatggccatt attatcgttt
    16921 ggcgagtcct tttgaagaaa atattgccgc atggatgttt gtgtctccta aacaagatga
    16981 agctattgtg tttttaggga ggatactagc ttcagctcaa ccagcgttcc atgaagtata
    17041 tctgatgggg ttagatgatg aggcacttta tcaggaacag acctcgaagc ggatattttc
    17101 gggggccgaa ttgatgacag ttggacttta cttccccgat tttcaaggtg atttccaaac
    17161 agaactgctt catttcaaaa agttatgaga gagaaggaaa aaagtatgaa agcaaatata
    17221 aaatggttag atgatccaca agtttttaga gtgaaccaat caaatgcaca tagcgatcat
    17281 gtttgttatg caacaaggga agaagtagaa accacatcct cccttaaaca gtctttaaat
    17341 ggatcatggc gtttttgtta ttcgccaaat ccttcggtac gaccaaaaac attttataaa
    17401 aaagattttg atgcctgttc ttttgatgaa atcactgttc ctagtcatat tgaattagca
    17461 aattatgaca agatccatta catcaatacg atgtatccgt gggagggaca tgtatttcgc
    17521 cgtccccctt tcacgacaag ccctgagaaa gaggcggaag ggatcttttc agctgctaaa
    17581 gacaatccgg ttggttcgta tttgacgact tttcgattag aagaaggtct aaagggaaaa
    17641 caaatctcga ttcattttgc tggtgtagaa caagcaatgt ttatttggtt gaatggtcat
    17701 tttatcgggt atgccgaaga ttcgttcact ccgtctgaat ttgatttaac accttatttg
    17761 gaagaagaaa atattctagc tgtggaggtt tacaaaagaa gtacagctgc attcttggaa
    17821 gatcaggact ttttccgatt ttttgggatc ttcagagatg tcttcttagt tgctcgagga
    17881 caaaaccatt tagaagatct ttggatcaaa cctcgcttag atgacaaatg tcaaaatggc
    17941 aaacttgaag tagatttgtc ttttgagcaa gtagccaaaa acagtcagct gaatgtagcg
    18001 atactagatc ctgatgggaa ggaaatctat caacagcaat atccgttaaa agagcaaatg
    18061 acgattcgca tcgatggatt tgaaaatgtg gcactgtggg ggcattttca gcccaattta
    18121 tataagcttg aattggagac gatagacgaa gaaaaaagag taacggagta tattgactat
    18181 tccttcggtt ttcgtaaaat cgaaatcaaa gataaaatca tctacttcaa tggaaaacgg
    18241 atgattatca acggggtaaa tcgacatgag tggcatccga aaaaaggacg cgccatcact
    18301 atagaagata tggaaaaaga tttggctatc ataaaaaatg caaagatcaa cggcgtacgg
    18361 acatctcatt accccaacca gatcacttgg tattatttat gcgaccaagc aggaatccat
    18421 gtgatggcgg aaacgaacct cgaatcccat ggctcatggc aaaaaatggg cgcatttgag
    18481 ccttcttaca acgtaccagg tgcagtacct cagtggcagg cggctgttct tgatcgagca
    18541 aaaacgaatt tcgaaacatt aaaaaaccat ccaagtattc ttttttggtc tcttggaaat
    18601 gaatcttatg cggaagaaaa cattcgacaa atgaacgatt acttcaaaaa agtagacccc
    18661 gatcgacttg tccattatga agggaatttt ccaaatccag cattcgaaga ttcgatttca
    18721 gatgtaaaaa gctgcatgta tgcgccacca gaagagatca ttgactacct tgaaaataaa
    18781 gcggaaaaac cgttcatctt atgtgaatat atgcatgaca tggggaattc gttaggtgga
    18841 atggattctt acatgacgtt gcttgatcgt tatgaaatgt accaaggagg ctatatatgg
    18901 gattttaaag atcaggcgat ccaagtgact gatccaatca caggcagaga agtgatgcgc
    18961 tatggcggtg actttgacga taaaccaagt gactatgaat tctcagggaa tgggatcgtt
    19021 tttgcagatg gacaagaaaa acccgccatg caggaggtaa gatattatta tgaaaaatac
    19081 agtaaataaa agtcatatgg atacggaaaa agttgcaatc gtcttcggcg actgtacatt
    19141 aggtgtcaaa tcggggaata cgcattatat tttttcttat acaagaggcg gactggaatc
    19201 gctcaataaa aacggaaaag aatggctata ccgggaaaca cttccagttt tttggcgagc
    19261 aacgaccgat aatgacagag gtagcggatt tggatttaaa tccggtatgt ggttaggagc
    19321 cggattgtat ccaaaagtca aagaaataga agtactggtg gacaataaaa agatcgattt
    19381 gccgattgct ccagtaaata atcaatattc aagtaatgaa tacgctgaaa cggttgaaat
    19441 catttttaca ctggaaatca acacagtacc aaaaacccaa gtgacaattt cctaccaaat
    19501 cgctggagag ggtaaaatgc ttgtcaatat gcattattat ggacaaaaag gattaccgga
    19561 attacccgca ttgggtttaa ggttcattat gccaacgaaa gcaaaatttt ttgagtatga
    19621 aggcctatca ggggaaacat atccggatcg gatggctggt ggtgtcgaag gtatttataa
    19681 agtggcagga ctccccgttt ccccatacct tgttccccaa gaatgcggca tgcatatgga
    19741 aacgaaatgg gtagaactaa caagaaatca aacgttggat aatacggata aagatcaaga
    19801 agatttttcg ttgcgatttg aacagcagga tacgccattt gctttttccg ctttaccatt
    19861 caaagcagaa gaactggaac aggcaacaca tatagaagaa ctaccgccag aaagacgaac
    19921 ggttcttacg atttttgcaa aagtaagagg agtcgggggt atcaatagct ggggagcgga
    19981 tgttgagtca ccttaccgaa tcagcagcga agaggaccat gaaatcggct ttattattca
    20041 gtagcaatct ttagattatt atttgaaaaa cggagtttac aagacaaagc tgctttattc
    20101 tatatgtcat gaacaagaga gtaaaaaagt tctcagatac ttttgttcat gactattttc
    20161 ttcaataaaa ccaattgtta tttttctgaa aaacaaagtt aactatcaga aaattaaaaa
    20221 aagtttcata aaaatgattg a
//
LOCUS       DAJSMI010000036        19995 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJSMI010000036 DAJSMI010000000
VERSION     DAJSMI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.036"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            4..816
                     /locus_tag="ON430_001050"
     CDS             4..816
                     /locus_tag="ON430_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCR3218978.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ON430_001051"
     CDS             813..1613
                     /locus_tag="ON430_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR3218979.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ON430_001052"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ON430_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCR3218980.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ON430_001053"
     CDS             3110..4819
                     /locus_tag="ON430_001053"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR3218981.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="ON430_001054"
     CDS             5031..9383
                     /locus_tag="ON430_001054"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCR3218982.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="ON430_001055"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="ON430_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR3218983.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="ON430_001056"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="ON430_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR3218984.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="ON430_001057"
     CDS             11221..11514
                     /locus_tag="ON430_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCR3218985.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="ON430_001058"
     CDS             11511..11822
                     /locus_tag="ON430_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCR3218986.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="ON430_001059"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="ON430_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR3218987.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="ON430_001060"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="ON430_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR3218988.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="ON430_001061"
     CDS             complement(14623..14844)
                     /locus_tag="ON430_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3218989.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="ON430_001062"
     CDS             15292..17655
                     /locus_tag="ON430_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HCR3218990.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="ON430_001063"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="ON430_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR3218991.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="ON430_001064"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="ON430_001064"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCR3218992.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT         6862 a         3511 c         4389 g         5233 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcattta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg caactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcattaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgatc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg acagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaaa cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgcct atttgtattg gaaaagccac
    18181 aaaagttatc gagtatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaagaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aagatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtgaaac gattcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggcaaagt tgtcagtatc tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtact
    19021 gggatttttt gacggcgtgc atattggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catcgattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgaggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacagttc atttgcctaa
    19621 agaaggtgtt tatgtatcgg aaataaaaat tggggaaact tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagaat tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcagta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaaaa aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aatttcaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagc
//
LOCUS       DAJSMI010000037        19859 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJSMI010000037 DAJSMI010000000
VERSION     DAJSMI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.037"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            108..656
                     /gene="aac(6')"
                     /locus_tag="ON430_001065"
     CDS             108..656
                     /gene="aac(6')"
                     /locus_tag="ON430_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR3218993.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            911..1714
                     /locus_tag="ON430_001066"
                     /pseudo
     CDS             911..1714
                     /locus_tag="ON430_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1704..2657
                     /locus_tag="ON430_001067"
     CDS             1704..2657
                     /locus_tag="ON430_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3218994.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            2669..3679
                     /locus_tag="ON430_001068"
     CDS             2669..3679
                     /locus_tag="ON430_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3218995.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(3964..4785)
                     /locus_tag="ON430_001069"
     CDS             complement(3964..4785)
                     /locus_tag="ON430_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3218996.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4882..5160)
                     /locus_tag="ON430_001070"
     CDS             complement(4882..5160)
                     /locus_tag="ON430_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218997.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            5428..7437
                     /gene="metG"
                     /locus_tag="ON430_001071"
     CDS             5428..7437
                     /gene="metG"
                     /locus_tag="ON430_001071"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR3218998.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(7916..8503)
                     /locus_tag="ON430_001072"
     CDS             complement(7916..8503)
                     /locus_tag="ON430_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3218999.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            8678..10000
                     /locus_tag="ON430_001073"
     CDS             8678..10000
                     /locus_tag="ON430_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3219000.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            10155..10931
                     /locus_tag="ON430_001074"
     CDS             10155..10931
                     /locus_tag="ON430_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR3219001.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            10924..11493
                     /gene="rnmV"
                     /locus_tag="ON430_001075"
     CDS             10924..11493
                     /gene="rnmV"
                     /locus_tag="ON430_001075"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCR3219002.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            11486..12370
                     /gene="rsmA"
                     /locus_tag="ON430_001076"
     CDS             11486..12370
                     /gene="rsmA"
                     /locus_tag="ON430_001076"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR3219003.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            12821..13450
                     /locus_tag="ON430_001077"
     CDS             12821..13450
                     /locus_tag="ON430_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219004.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(13574..14683)
                     /gene="ugpC"
                     /locus_tag="ON430_001078"
     CDS             complement(13574..14683)
                     /gene="ugpC"
                     /locus_tag="ON430_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCR3219005.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            14892..15314
                     /gene="mgsA"
                     /locus_tag="ON430_001079"
     CDS             14892..15314
                     /gene="mgsA"
                     /locus_tag="ON430_001079"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR3219006.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            15637..16173
                     /locus_tag="ON430_001080"
     CDS             15637..16173
                     /locus_tag="ON430_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR3219007.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(16213..17976)
                     /locus_tag="ON430_001081"
     CDS             complement(16213..17976)
                     /locus_tag="ON430_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3219008.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(17979..19694)
                     /locus_tag="ON430_001082"
     CDS             complement(17979..19694)
                     /locus_tag="ON430_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3219009.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         6434 a         3635 c         4012 g         5778 t
ORIGIN      
        1 ataatgaaag aatagagata ctgattggta gctaaagatt tttgagcgag aacatttcgt
       61 tttttcaaaa ggactgttag aataagcaag aacaaggggg aagacgtatg ataatcagtg
      121 aatttgaccg taataatcca gtattgaaag atcagctttc tgatttactg agactgactt
      181 ggccggaaga atatggagac agctcggcag aagaagtaga agaaatgatg aatccagaac
      241 gaatcgcggt agcagcggta gaccaagatg agttagtagg atttattggt gcaatccctc
      301 aatacggtat cacaggttgg gaattgcatc cattagttgt agaaagctcc cgacgaaaga
      361 accaaatagg tactcgatta gtcaattact tagaaaaaga agtagcttcc agaggaggaa
      421 tcacgattta tttaggtacg gatgatttag accatggaac aacgttaagt caaacggacc
      481 tgtatgagca tacatttgat aaagtggctt ccatccagaa ccttcgtgaa catccgtatg
      541 aattctatga aaaattaggt tataaaatcg taggtgtctt accaaatgca aatggctggg
      601 acaaaccgga tatttggatg gcaaaaacga ttattcctcg accaaattct caataataaa
      661 aagaggttgt gacagaagta atcagcttca agaaataaga aggaatttac gaaaattact
      721 tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag ctacttttgg aacaccgtgt
      781 accagaggtt gtgaaaaagt cgcttagttt cgagtagtaa gaaaaatttt gtgaaaatgg
      841 ctttctacat ttttgcataa atttggctta ataccgagaa actggctttt gaacaccgtt
      901 tattcagata agggacatag cgatgatttt ccaagatcca atgacctctt tagatccgac
      961 gatgcccatt gggaagcaag tcgctgagtc ccttagaaag cataataaag tttcaaaaaa
     1021 agagggacaa aaagctgcat tagaattgct taaattagtt ggaatacccg aagctgagaa
     1081 acgtatcaac agttatccgc atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc
     1141 gattgcactg gtatgttatc cggaaatcct gattgctgat gaacctacaa cagcgcttga
     1201 tgtgactatt caagcacaga ttttggagct gctgaaagaa attcagacaa aaatcgatac
     1261 atcaattatc tttatcactc atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc
     1321 tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg gaagaaattt tctacaaccc
     1381 gcaacatccc tatacttggg gactgcttgg ttcaatgccg acattagata gtgcgaacga
     1441 tcgattgtat gctattcctg gttctccacc ggatttattg aacccgccaa aaggagacgc
     1501 attctatcca agaaatgaat tcgcgatgaa aatcgatgca gaacaagaac caccattttt
     1561 tgaactatca aaaacacaca aggcagcaac ttggctactt gctccgcagg cacccaaagt
     1621 gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt aaagagaaac aaaaacatac
     1681 ttacggtcaa ggaggaattg ctgatgccaa ataaactact tgaagttaaa ggattgaaac
     1741 agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt aaatgacatc agctttcaca
     1801 tttatgaagg agaaacattc ggtttagttg gagaatctgg aagcggaaaa tccactactg
     1861 gccgtacgat cattcgcttg aatgaaccta ctgatggtga gatattattc gacggtcaag
     1921 atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga
     1981 tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa agtcagagat atcattgccg
     2041 aaggaattga tgttaatgga ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg
     2101 atctattgag aactgttgga ttgaatccaa gccacggtac tcgttatcct catgaatttt
     2161 caggtgggca acgccagcgt attggtattg cacgagcatt ggcagtaaaa ccgcgtttta
     2221 tcatttgtga tgaaccaatt tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt
     2281 tgttgcaaga tctccaaaaa gaacatcaat tgacctatct atttattgca catgatttgt
     2341 cgatggttaa acatattagt gaccgaatcg gtgtaatgca taacggcttg ttattagaga
     2401 tgggaacaag tgatgaaatc tataatcacg gtgttcatcc ttatacagaa agtttgttat
     2461 ctgctattcc tctaccagat ccagaccatg agagaaaacg cagaagaatc aaatatcaac
     2521 ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct
     2581 atgcaactga gcaggaagtc ccatattacg caaaaaaact aaaacgacaa aaggaatctc
     2641 ttctggttgc taactaggag ggacatagat gctaaaagct tttaaattcc gtatgtatcc
     2701 gacagaagaa caaaaacagc aattgattcg tacttttgga tgtgctcgat ttacctataa
     2761 ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca ggagtagcca atttttcgct
     2821 cacacccgct acattaaaaa aagaataccc attcttaaaa gaagtagaca gtcttgcttt
     2881 agcaaatgca cagcttaatt tggatcgggc ttttcgcaat tatttcaagg gacgtgccag
     2941 cttcccaaag cttaaaacga aaaaaagtat gtggcaatct tatacgacca acaaccaaac
     3001 ccgtacgatc tatctggaaa atggctactt aaagttacca aaacaaaaag aactaatcaa
     3061 gataaatcag catcggccag ttgaaggatc gattcgctcc gctacgattt ctgctcgata
     3121 taatgaagaa ttttatgtcg cactactttg tgatgtttcg ccagtaaaaa aagaatcttt
     3181 agctaaatgg atcgggatcg cctatcatcc gaagacattg atccaaacat ctcgacctct
     3241 agaagtaact ttaccaaaat ttcaccagac agaagaaaag ctgcaacatg cacagcggaa
     3301 attgaatgtg aaagtccgct ccgctcacca tagaaagatc aggttagatc aggcaagcaa
     3361 ttatcagaaa caaaagagaa aagtaatgga tctttatctg aaacaaaaga accagcgaga
     3421 agattatctt gaacaattat ctgggaaatt ggtcaaacaa tatgactact tatttgtaga
     3481 atcttttcca aaagaagagg cacatgctga tttttctatt cacgactggc ataaattaat
     3541 aacaaaattg cgttataagt cccaatggta taataaaaaa tttcttttga tcaataccga
     3601 cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca
     3661 ttcggttatc aagggataaa aaatgaatga aactgatagg agcgttaggg ttagccttgt
     3721 taataaaaag tcttaatgac ttttgtttct aggaattttt cttttgaaga aaagctcact
     3781 aattatgaca aaccaataaa acaatagcag aaaaagttcg tacgagcttt ttctgctatt
     3841 gttaattttt tattcagaaa gtttacgaat acatcaaaat tctgaaacaa aaaacgtaac
     3901 tcacaaagag ttgtttttgc gttttttctt gaatgattag gtctgaatct tttgctcaca
     3961 accttattca tggtgccccg gcttgagctg atagattttt gaaggacggc cgattcctac
     4021 aggacgttca ccgatctcat cgaaatattg gagcatagct ttcttaaaat tggaatgatc
     4081 gatcgtttta taatcgatcc ctaagaattt agcaaatact tttcgagctt ccgtaatcgt
     4141 gaaatcttta cctaaaactt gtagaacttg cggctcgtgt tccattttat tgactacacg
     4201 gttgaatgct ttcaagatga tttgactatg gtcaaaagcc aaagtatctt tgccagaaga
     4261 ctctccagtg actaaatcaa gcgagatagc cacttctccg ctagataaat ggatcttgtt
     4321 actatgacgt tctagtgtga accagcgtac ttccttggca tcatctccgg cgattagcgg
     4381 ttcttctcca ataaaggcta agtagctgac tgtgacgacc cagcctcgcg gatctcgatt
     4441 aggcgtactg aatgtatgca gttgctcaat attttctttt gaaataacca caccagtttc
     4501 ttcctttgtt tctcttaaga cgctttctcc agttgactca tccttttgga caaagccacc
     4561 aggcaatgcc catgaatttc gataaggatg cccttttctt tggatcaaca aaactttcag
     4621 ctgatcttct tctttgttat agcacatcag tacgatatcg acagtcaaag agggcttgtc
     4681 atattctgga cgttcctgtt tttgatacca gttaagaaat tcttcttcac ttgctttatg
     4741 ttcataatat aatttttctt cttctttaga agcaaaacgg ttcatctgac cacctccatt
     4801 gacagtaaaa gtacttttta ccttattgta gcagactctg aaaaaaaacc aaggatttac
     4861 ttgaaaaatc taggttatca tttaaatatg aaaaataaat ggaagtatga gattgaataa
     4921 aaagaaaagc agataaaacg gcaatacttt ataccgctct tcacgaatgg caaagaagcc
     4981 acaaataacg atatatacgt caatgataaa acgcaaaatc caagctgctg tcataaaact
     5041 gttcaaatgc ccatggaggt taaaataacc atacatacga atataaaaat agctgaatat
     5101 taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa aaaagatgtt tcttctgcat
     5161 gattgttcca gtcccttact aagatagata gtctttatta taccacgtgt gaggggagaa
     5221 ataaaaagcc gttttttagt agatatgaaa caaaacaaaa acccgcattt attcaaccta
     5281 ttcatccttt taaaggatag attgaacgta gatagaagtc gtttatccag tctttttcat
     5341 tttgaaaaaa agctaacagc atgctttttt tatggtatac tagcaaggaa acgaattcgc
     5401 atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg aaacttttta tatcactacg
     5461 ccaatctatt atccaagcgg caaacttcat atcggcaact cgtatactac gattgcatgt
     5521 gacgcaattg ctcgttataa acggttgatg ggctttgatg tgttttatct cactggagtg
     5581 gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac taggtgtaaa accgcaagaa
     5641 tatgtagata aaatggcggc tgatgtcaag aaattatgga aaacgcttga tatcagttat
     5701 gataaattca ttcgcacgac agatgattac cataaagcag ctgttcaaaa aatatttgat
     5761 cgtttattag aacagggaga tatttactta ggtgaatatg aaggctggta ctctgtttct
     5821 gacgaagaat tctttacaga aacacaactt gcagaagtgt acaaagatga cgaaggtaac
     5881 gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat tggtaaaaga agaatcatac
     5941 ttcttccgca tgagcaaata cgcagaccgt ctattggaat actatgaaga acatcccgaa
     6001 ttcatccagc cagaatctcg taaaaacgaa atgatcaaca actttatcaa accaggatta
     6061 gaggatcttg cagtttcacg aacaactttt acttggggag taccagtcaa gagtgatccg
     6121 aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt atattactgc actaggatat
     6181 ggctccgaag atgaaagtct attcgaaaaa tactggcctg cagatgttca gatggtcggt
     6241 aaagaaatcg ttcgctttca tacgatctat tggccaatca tgctgatggc ccttgatttg
     6301 cctctaccta aaaaagtatt tgggcatggc tggctattga tgaaagacgg aaaaatgtct
     6361 aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg aacgttatgg tttagatgca
     6421 ctccgttatt atttgcttcg tgctgttccg tttgggtcag atggtgtctt tacgccagaa
     6481 gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc tagggaactt attgaatcga
     6541 acgattgcta tgatcaacaa atattgtgat ggactcgttc ctgactatgc ctcacttgtc
     6601 actccatttg atagtgaact gtctacgacg gctgctaacg ttgtcggacg ttaccatgat
     6661 gcaatggaaa aaatggaatt caatacagca ttggctgaaa tctgggtgct gatttctcgt
     6721 gcaaacaaat acattgatga aacagaacca tgggtattag caaaagacga agaaaagaaa
     6781 gctgaattga atagtgtcat gatccattta gctgaatctt tgcgtatcgc agcgatatta
     6841 cttcagccaa tcatgacaga aacgccagag aagatcttca atcaactagg attggactct
     6901 gaaacaatga atcttgaagg attacacttt ggcgaatttc cttcaggaac aaaagtagtt
     6961 gcaaaaggta caccaatctt cccacgttta gatatggaag aagaagttgc ttttattcaa
     7021 gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag tcaaatggga tccagaagaa
     7081 acagaacttg tttcaacgaa agaaaaacaa atcaaatttg atgtatttga aaaagtagaa
     7141 ctaaaagtag cagaagtcat caactgtcaa aaagtagaag gggcagataa gttgctgcaa
     7201 ttccgactag atgctggaga cagccaagac cgtcaaatct tgtcaggaat cgctgaattt
     7261 tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca ttgtagcaaa tctgaaacca
     7321 cgaaagatgc gtggacaaat cagtcaagga atgatccttt ctgcagaagc accagatggc
     7381 tctttacaag taatcgaagc accaaaatcc atgccaaatg gctcagaaat cgcatagaaa
     7441 ttcaaagggg gtatgacact agttgtgtca tacccccttt ttccgaaatt tgagaagcaa
     7501 ttttcgtaaa ttccttctta ttacttgaag ccaatcatat aatacagaaa cagagaacta
     7561 tccaaaataa atcctagaaa aagaagtacg agcttcgctg atttttgtta atttagaata
     7621 atactaatag gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc gggcagattt
     7681 taattgattc atctggtact cttttttatt gtccttacat tcataaagtt tgaatgcaat
     7741 gactttatta catctatatt tatatctaac agtagcgttg atgtgttaat tataagttct
     7801 atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata tagataaaaa
     7861 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
     7921 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
     7981 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
     8041 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
     8101 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
     8161 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
     8221 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
     8281 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
     8341 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
     8401 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
     8461 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
     8521 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
     8581 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
     8641 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
     8701 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
     8761 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
     8821 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
     8881 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
     8941 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
     9001 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
     9061 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
     9121 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
     9181 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
     9241 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
     9301 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
     9361 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
     9421 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
     9481 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
     9541 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
     9601 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
     9661 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
     9721 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
     9781 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
     9841 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
     9901 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
     9961 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
    10021 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
    10081 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
    10141 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
    10201 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
    10261 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
    10321 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
    10381 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
    10441 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
    10501 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
    10561 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
    10621 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
    10681 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
    10741 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
    10801 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
    10861 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
    10921 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
    10981 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
    11041 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
    11101 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
    11161 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
    11221 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
    11281 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
    11341 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
    11401 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
    11461 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
    11521 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
    11581 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
    11641 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
    11701 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
    11761 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
    11821 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
    11881 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
    11941 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
    12001 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
    12061 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
    12121 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
    12181 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
    12241 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
    12301 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
    12361 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
    12421 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
    12481 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
    12541 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
    12601 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
    12661 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
    12721 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
    12781 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
    12841 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
    12901 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
    12961 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
    13021 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
    13081 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
    13141 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
    13201 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
    13261 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
    13321 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
    13381 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
    13441 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
    13501 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
    13561 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
    13621 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
    13681 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
    13741 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
    13801 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
    13861 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
    13921 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
    13981 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
    14041 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
    14101 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
    14161 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
    14221 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
    14281 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
    14341 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
    14401 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
    14461 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
    14521 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
    14581 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
    14641 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
    14701 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
    14761 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
    14821 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
    14881 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
    14941 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
    15001 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
    15061 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
    15121 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
    15181 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
    15241 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
    15301 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
    15361 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
    15421 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
    15481 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
    15541 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
    15601 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
    15661 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
    15721 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
    15781 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
    15841 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
    15901 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
    15961 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
    16021 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
    16081 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
    16141 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
    16201 gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc
    16261 tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa
    16321 tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc
    16381 ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct
    16441 ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa
    16501 cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt
    16561 ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc
    16621 tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag
    16681 ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg
    16741 gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa
    16801 attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg
    16861 ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac
    16921 atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa
    16981 atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc
    17041 ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga
    17101 gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa
    17161 taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg
    17221 gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag
    17281 cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt
    17341 accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa
    17401 atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca
    17461 aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat
    17521 gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc
    17581 catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag
    17641 tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc
    17701 taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc
    17761 accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat
    17821 ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat
    17881 tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg
    17941 gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg
    18001 acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca
    18061 atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg
    18121 gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct
    18181 gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc
    18241 aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc
    18301 atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt
    18361 ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca
    18421 cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga
    18481 tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca
    18541 tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg
    18601 attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa
    18661 tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg
    18721 ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc
    18781 cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt
    18841 gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta
    18901 ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc
    18961 aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct
    19021 agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg
    19081 atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct
    19141 tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa
    19201 ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc
    19261 actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct
    19321 agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga
    19381 ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta
    19441 ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca
    19501 atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt
    19561 tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct
    19621 tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt
    19681 aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta
    19741 ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga
    19801 aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaaagaa
//
LOCUS       DAJSMI010000038        19491 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJSMI010000038 DAJSMI010000000
VERSION     DAJSMI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.038"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            133..3660
                     /locus_tag="ON430_001083"
     CDS             133..3660
                     /locus_tag="ON430_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCR3219010.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ON430_001084"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ON430_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCR3219011.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ON430_001085"
     CDS             7448..7789
                     /locus_tag="ON430_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3219012.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ON430_001086"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ON430_001086"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR3219013.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="ON430_001087"
     CDS             9146..11566
                     /locus_tag="ON430_001087"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR3219014.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ON430_001088"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ON430_001088"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR3219015.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ON430_001089"
     CDS             13000..13737
                     /locus_tag="ON430_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3219016.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ON430_001090"
     CDS             13751..14566
                     /locus_tag="ON430_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR3219017.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ON430_001091"
     CDS             14579..15226
                     /locus_tag="ON430_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3219018.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ON430_001092"
     CDS             15242..15898
                     /locus_tag="ON430_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3219019.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ON430_001093"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ON430_001093"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR3219020.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ON430_001094"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ON430_001094"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR3219021.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ON430_001095"
     CDS             18240..18758
                     /locus_tag="ON430_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3219022.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="ON430_001096"
     CDS             18865..19365
                     /locus_tag="ON430_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3219023.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6557 a         3346 c         4184 g         5404 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga a
//
LOCUS       DAJSMI010000039        18821 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJSMI010000039 DAJSMI010000000
VERSION     DAJSMI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.039"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..207
                     /locus_tag="ON430_001097"
     CDS             <1..207
                     /locus_tag="ON430_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR3219024.1"
                     /translation="TNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVIPENWRK
                     SLQNDQLLKTISQRFCDTVLSQRI"
     gene            276..542
                     /locus_tag="ON430_001098"
     CDS             276..542
                     /locus_tag="ON430_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3219025.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            532..801
                     /locus_tag="ON430_001099"
     CDS             532..801
                     /locus_tag="ON430_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3219026.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            1489..2256
                     /locus_tag="ON430_001100"
     CDS             1489..2256
                     /locus_tag="ON430_001100"
                     /inference="COORDINATES: protein motif:HMM:NF025424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219027.1"
                     /translation="MKNGTIKRMADLKSALREENQKKQLSIFDETLGYAAIDFRIDHQ
                     QKGERSISWVTLIAAPSVKMKTVGEFLERLEVYPDQTVVATVGLEGGYSPVHLAMDGL
                     GKETWLVLCDEIAYHNFFSPPEHSELEVEFDLKGAVKEHLKLQLETSKNPTETRLAGW
                     LEQQEDSELLIGVLKEEKSLSELVQFCIQKASASKGKQSAMMVSDEVVFQWVREYYTT
                     DIEMKNSSSVKGTMQTTPTPQVIETERKEEEQQISLF"
     gene            2262..3587
                     /locus_tag="ON430_001101"
     CDS             2262..3587
                     /locus_tag="ON430_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HCR3219028.1"
                     /translation="MKIAYDYPFKPLTPPKGFFKWCSSQITGYRWANKERTIVSTTRP
                     EAPIISKRLTKASNLNRSERCYPFALVLSSKTRVEIQSFGIWVGIENGVETYEIARTN
                     LECFCNDVHYQQHYFRNKWYGGLLNHGYMTGSYTNTQFYPNKWREKLAKNSPLKYCNL
                     KDISWYHLAHAYKYRREIEFLKLIHADKFADDMLFSAMHVIRDRVRGKSIDYRTVNMK
                     WLRKHKGILKNSTITFCEMRLKEAIRKCHGKPIAGIEEILTVEDLKRIPTCVGLTSFQ
                     KWAVAQDLDFGMYVDYLNMLDELGIGVDKENAMPKEFRAAHDEKAKLVGVKENKEYEK
                     EFNERVLQLKGLEKRLGDVQIVAPKEMGEMLVEGQNLRHCVGGQYYIRRHAKGETTIL
                     FVRKADKLDEPYLTLEMRNHRIVQLRGKRNQAAPPEIEQIIEKWVKTAN"
     gene            4239..4451
                     /locus_tag="ON430_001102"
     CDS             4239..4451
                     /locus_tag="ON430_001102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219029.1"
                     /translation="MDALRKLEVTLYNKQWFTLIGIEACLKNQDGFSAEEVLDQIQGC
                     LEEYKHVEGAMMEEYQQQNFAKLETV"
     gene            4549..4776
                     /locus_tag="ON430_001103"
     CDS             4549..4776
                     /locus_tag="ON430_001103"
                     /inference="COORDINATES: protein motif:HMM:NF037117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219030.1"
                     /translation="MRKTISLTSNNIKVSGHIGTWYVYASRVYHGRRLFLVEHETYGD
                     HAANLILDKTGNCVMEDVWNGWEDYEVYIES"
     gene            4909..5382
                     /locus_tag="ON430_001104"
     CDS             4909..5382
                     /locus_tag="ON430_001104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219031.1"
                     /translation="MKTGGFVMICRYCNKKVLKLEGGQYHCANCELDFFENQLIEFRS
                     GLYREPTVETDPFFFQYTTTKELLQMTYVELLSRLKLARGVYRELRKKVYGHNSQADD
                     LDRENLKAVTSAKLVIENIMIEKHGYYPASVFESVIDKEVLKNKKQMREVEKKHG"
     gene            5375..5626
                     /locus_tag="ON430_001105"
     CDS             5375..5626
                     /locus_tag="ON430_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219032.1"
                     /translation="MVKFEDQPKQGYTGIRTLETIASVFEDIQASGDMGQGEKNLRYL
                     QLMNELEEDHGAMIVNPTKTDLNLPAVALYRKVSQARVL"
     gene            5651..5959
                     /locus_tag="ON430_001106"
     CDS             5651..5959
                     /locus_tag="ON430_001106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219033.1"
                     /translation="MDKSKEIIFMPDYLVKDHTVLVLDSANNLHGFLSIESTDDHFVF
                     TNDIDLLKTVPKIIAEELATDIRASEGDYIPVTNKDTGEVSELGSDDLQIVFSAVEWC
                     "
     gene            6033..6497
                     /locus_tag="ON430_001107"
     CDS             6033..6497
                     /locus_tag="ON430_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219034.1"
                     /translation="MNEPDMTNQFRVGEVILQRMIDRKEFEDIYRSVTRKNTVDAYYF
                     DAGRRIVTVFAYYLGSISGSSVDEERIIGELMALMATMERDSIPGVVVWDKISKLPGT
                     PEHSMQLASEKAIEDTFTFLLAAAKAEEPYYLNGGTETIVSMIAAEVEQDCR"
     gene            6516..6944
                     /locus_tag="ON430_001108"
     CDS             6516..6944
                     /locus_tag="ON430_001108"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219035.1"
                     /translation="MLKCYGEAELLEMLKRNEFEEDSPIRSVEDLLGHLDQASPMMWV
                     TVYVPEVQSVYGIHRETLISTGQSHRSTSKPVALLEATGYQTSEHVFVSELAALLQME
                     VVPKNREIFELQLFERKRGKYMLRRWQMIGDQPVLIAQRG"
     gene            complement(7114..7980)
                     /locus_tag="ON430_001109"
     CDS             complement(7114..7980)
                     /locus_tag="ON430_001109"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCR3219036.1"
                     /translation="MITLKNHKLELLQFTDIHIGQAPFNEEDQKTFAMIDQTLAKTTA
                     DLIVITGDLIWSDGVIEPTKGLEALAEIFNKYPIPLAITYGNHDSEETIDRHDLHELE
                     KKLFNHLALKANQFFDPNQKECFTIEIKDDNQLVNVLYFIDSGANALIDYESYDWVSL
                     EQIKWYDETFAKYQKINHTKDLLFLHIPLPEYLQAGERIVEGRFWEMNPRISAPKLNT
                     GLFSHLLENNHLLGTFCGHDHDNNFEGIYLGQRLIYGNVTGYNCYGDLPRGYRKITLK
                     PDQMTTSIETFE"
     gene            8161..9108
                     /locus_tag="ON430_001110"
     CDS             8161..9108
                     /locus_tag="ON430_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003729435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3219037.1"
                     /translation="MKQDSYSVKDANVILSKLILGSSDYLKLDNKDKVDRMFTRYVEE
                     GGRTFDTARHYRESEAVIGSWLKDKKREDYTIITKGCHPVREAPDTPRVNPESILADL
                     TTSLEMLATDYVDVLLLHRDDPSQPVGPLMETLSQLVDEKKIRSFGVSNWELPRIIEA
                     KKYCEDNGLNPLSFNSPNFSLAKVNEPRWENCVTADDLMIAWHEETDFPLISWSAQAE
                     AFFGNRFSPDDVNDPEMAEFVAVYFNDLNWKRLVICEELAKEKGVKPIQISLAYVLSQ
                     TFPSYATIGPEEEWQLLDSVEAMNIQLSAEEIMRLKKGE"
     gene            9114..9878
                     /locus_tag="ON430_001111"
     CDS             9114..9878
                     /locus_tag="ON430_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3219038.1"
                     /translation="MDKTKIAAQMYSVRKELEKDPENTFKSLKEIGFEAVQLDGMRGH
                     KETEIKALVDKYGFKIAGMHIKHDRFFNDRDGIISESLLFGCKTIYDKYIDDEEQHTK
                     GYIQTKQQLVTTQATLGKLGFRIGLHCPEYDFNQTVEGRTILDYITDPVFGTAVYAEP
                     DTYWISVAGKDPLEEFRKFSGRAPIVHLKDYIKGFAPEDMDNNLTELGNGDVDFKAIV
                     EWGEKSGVEYYCIEQDYSKIGIFNSLAKSFDHLMSI"
     gene            9895..11148
                     /locus_tag="ON430_001112"
     CDS             9895..11148
                     /locus_tag="ON430_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3219039.1"
                     /translation="MKKNSRIVMMGVALVSGLVLAGCGNGSASNSDKVELSINYYNGA
                     ISKKTMDNAKKEFSDYKLSFKQIPANDDFDTKLKASLNSKSAPDITAINSNISDYLPY
                     ADKFVNLLDYDSETLAADFVEWKWDSCFTNDKKTQIAMPIDIGPTALFYNVDAFAKAG
                     LPTNDQEISKALKDEESLMAAAKQMKEKADMPFFQSSLSLLQSKNRTMTEHIYDENGK
                     LTYANGQLKEIWDYAVKAEKSGYTLGTKGNSADGANSVQKGMFGGMIEASWGINDLAE
                     NGVSPKQWMIAKSPFTASNYGGSYLAVIKTSSHPKEAAEVVKYLTNEDSQRANYEELA
                     LFPSNTKVYDDSFKAAKNELFGDEEFNHYFIDAANELEYVPSDPRESAAFKCFEDQII
                     LVAEQGKDPEKAWNDAVKKVEEMSK"
     gene            11231..12154
                     /locus_tag="ON430_001113"
     CDS             11231..12154
                     /locus_tag="ON430_001113"
                     /inference="COORDINATES: protein motif:HMM:NF012738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3219040.1"
                     /translation="MEIGEMMNSESKSKKKFSFDKVAPYVFVSPFYILFLIFGLFPVI
                     FSGVLAFSKWDGLGDIQFVGLDNFTRLLRDADFYLSVKNTLIIWAVGTFPMLFLALIL
                     AFLVNLKFIKHKELLKSIFFLPNITSVVAVTILFGLIFSNLDGLANALLQFLGFEPIK
                     WVTSPFWVRIVIALINIWMYLGYNMIIYLTGITKIPNDYYEAAVIDGATNMQIFWNIT
                     IPQLKPIILFTVMMSTIGGLQVFSEAQVLVPQGATPEGGAMTIVYYLYNTAFTNNEYG
                     YGSAVAWALVLIILVFSLINWYLTTYRIKGE"
     gene            12157..12981
                     /locus_tag="ON430_001114"
     CDS             12157..12981
                     /locus_tag="ON430_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3219041.1"
                     /translation="MSNRIFKILVRAFLAIGVFLSIFPFYWMIVMASKDNSEIYHIPP
                     DLMLGSELMNNIKTVFTETTFFSSLLNTLFVALTSTLLILFFDSLAGFSFAKLNFKGK
                     AFLFTFLLGTMMIPGQLNIIPSFYLMDKFGWVGSYKALIIPGMANAFGIFWIKQYCTD
                     AIPDSLLESAKLDGCTLFKAYYKVVLPIMKPALAFLALYSFMGAWNDYMWPLIILNDE
                     SKYTLMLALTQLQGLYKTNYPLVITGTLLATLPLLLVFTIFSKQLISGITEGAVKQ"
     gene            complement(13094..13381)
                     /locus_tag="ON430_001115"
     CDS             complement(13094..13381)
                     /locus_tag="ON430_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3219042.1"
                     /translation="MKEKNPYYRLKYVRIYKRRGKKRAIITIARMMLTAIYHMLSTGE
                     VWNPTDLYKIDMPDNLVQKQKDKAIKQATKLLISEGLLPDDFVRKDLEPAI"
     gene            13717..14307
                     /locus_tag="ON430_001116"
     CDS             13717..14307
                     /locus_tag="ON430_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219043.1"
                     /translation="MEVNMKEKFIALQAKLMDLILLSGLILIGAMTIIGFFYGIVAAF
                     VYLETKSEDKWQVLKKRQTILISLLLEMSAVFVAGIFFLNARLLSQLQGGATIIISFF
                     LAVCTLILIPLYFILLHKLAVGTIFTKETFLNSVKVILIGFPTWFTQIILMIALSVLT
                     YLFPALSIITVGYLITRTNKIINVQKMKLASLQEVY"
     gene            14307..16316
                     /locus_tag="ON430_001117"
     CDS             14307..16316
                     /locus_tag="ON430_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3219044.1"
                     /translation="MKLSQREIDLLKLALDGEEETTVPQLKKQLKLSVHQIRYAIDKV
                     NRFLIEKKLNQIFIEKDRLVIEQRRKIKKEIDFFISHSTPEQFKFTAEQIEYFISLKL
                     LLSDQRLPINYFVETLSTSRTSVVNVLDRITQELAEEEIELSNVPRKGYMIEEYSFKR
                     FSYFITILKKMINIREIYSFYYQDNLYSKAGDLVFFDLFDLDILFDSLGETIQYSKQM
                     ASEIDDTNFLFLTILNYKYRELINAFEMTDRTEKLQMISSFISEIKQNNPNQMENNNL
                     AKRLIRYINRELADVFEVKDFKVASNALLYHIQRFIFRQENGIPFRDIDSEVLVKDHK
                     NLYYVIFAAIRSFQNGLFESVEAPEAVLITLYYINEIEKNAPKVFKAPRILIICGEGR
                     AISSVLKIKISNLIDSKWIDTISVFEFETPLIDHYDLIITTVRLPEVESDKVLYSDNV
                     FSESFFEQIKLKLSVGGYSFATQRLGKLSQIMNAIVEEVNDDINMNRLEAKIIKILAN
                     NLLQNNRPNEQKFIFDASMLKYHQAAQTWQNAIRISGQELLAKHSIEEQYVNNIISNI
                     ENFGPYMIIAPGVLLAHAGEQDGVLNNGFSMHVFHEPITFPGENVFPVSVIMTIAVKD
                     RTSYHVVERIVQWVLDEETIFSLADEKNRQRIEQMIRNILMMQEV"
     gene            16318..16629
                     /locus_tag="ON430_001118"
     CDS             16318..16629
                     /locus_tag="ON430_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3219045.1"
                     /translation="MTMTTDEISFALISKAGTAFSMMIEALGDAREKDFEKAEEKKQE
                     AEALMNEAHNLQTNLIVNEVNGDDNQINALLIHAQDTLMNTILMSTIVDEFLNLYKAG
                     E"
     gene            16631..16966
                     /locus_tag="ON430_001119"
     CDS             16631..16966
                     /locus_tag="ON430_001119"
                     /inference="COORDINATES: protein motif:HMM:NF014368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3219046.1"
                     /translation="MEKLSICLACNLGSSTSILVNKMKQVAAGSQKLSSFDLMIDAIP
                     AGNISTEQVKDYQIIMLGPQIGHRRNEIEQKVASINIPVTVIDAADYGSMNAANILKE
                     AIYQIKTAN"
     gene            16979..18214
                     /locus_tag="ON430_001120"
     CDS             16979..18214
                     /locus_tag="ON430_001120"
                     /inference="COORDINATES: protein motif:HMM:NF014437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3219047.1"
                     /translation="MTLDYINQVVGKIQTKMIPTLGKIEGNQVFKSMKKTFLYLNYVL
                     LISSVLAILKLVNEKFIHQQQIADLTSKLLLLLMDNFGWFFLGILCFLMFQEKGKFHY
                     YLCSAALYLMFSAKDLNLLSASKIETLFLALLALLVSFVLVSGYQLVLRGIKKIIKTP
                     MEFLDNLLLMIYFSVIFMVIYQILSQLKGIHLENILSWLNIDNPMMVFVIVFLEMFLW
                     YMGINGYGILAPIVLLFAISNLNANFQAIATGEAPQFIFTPNFWDYFLSVTGSGITGA
                     IVILSLLSKKTTLKNLGRTAASGTLFSVSEPIVFGIPVVMNPYFFIPFVIGTPILGVI
                     QWYVFKLGWVSLPTYFVADLPLPFSSFLATMDWRSLILTAATIALATLMYWPFYKAFE
                     KSYVEKNNDDKYQDLDLDF"
     gene            18230..>18821
                     /locus_tag="ON430_001121"
     CDS             18230..>18821
                     /locus_tag="ON430_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3219048.1"
                     /translation="MLKSFFKIFCGNFILGLAYAKLMVPNEIINGGVTSLSLIFSNLT
                     SLNITLFTNVITVTLLAVSLLFLGKFFFFSSVVSSVSYLLFFNLFYRLPFSLHTNVVV
                     DFLLAVLLIAIGYYCCLSENSSTAGLDVFAVILNKKIPKISVAVFLRYINIAVLVLGL
                     FSYGIKAVILGVLFSFSFTKVMDLLMEKGADPRKFFH"
BASE COUNT         5852 a         3235 c         4107 g         5627 t
ORIGIN      
        1 acgaatagtt atcaagcagc catattgact gcggtcaatt taggagggga tactgatact
       61 actgctgccg taactggtgc tcttgcgggt attctttacg ggggtgaagt gatccctgaa
      121 aattggagaa aatcgcttca aaatgaccag cttcttaaaa cgatcagtca gagattttgc
      181 gataccgtgc tatctcaaag aatttgaata atggatatga tgcaaatctt gtttgtacta
      241 acgttgtatt gacaaaaagg attttattta gtacaatgtt agtacaaatg atgaacggag
      301 gtagaatcat gtctcaatca accattacat ttaggattcc cgacgatgaa aaagaactgg
      361 tattagaata tgcaaaagca cacaattctt cattgaccga actttatcga aatgctgtac
      421 tggataaaat cgaagatgaa atcgatctac ggactttacg gagtgcaatt gaagtttcca
      481 aagagaaaaa agagagtggt atttctcaag aggaaatgga gaaactgcta aatgaattat
      541 aaagtcatct acatgccgca agcccagaag caattaaaaa aaatggacag aaaccaagct
      601 aaaataattg tcgcctggat cagaaagaat ttagaaaaca cgactgaccc gcgtcagcac
      661 ggaaaaggtt tgactggaaa taagagtggg gaatggcgct atcgaattgg aaactatcga
      721 atattggcta atatttgtga ttctgaaatt gaaatccaga tattctcaat tggacataga
      781 agtacaatat ataaaagata accgtttttg gaacgaagta gagtttcgaa agtcaatgta
      841 taatcttttg aattttgcat aataccacag gtagaaacta cccgatacta tcggttggtt
      901 tctgcttttt tatttggtct tgaagaagaa aacgagtgat ctgtggcaaa gaaagaaaca
      961 aaaaaaggac aaaactgttg cttcgcctgc ggtgcagtcg cccttggatc aaagcgcgtc
     1021 tgttttcttt tggctagtgg caaggtctaa ctgcttggtg gctacgtgcc ttcgccagtc
     1081 gcagctgcct tggttggctg cgtgcctccg ccaagtcggc cgacctcacc atttttgcct
     1141 acgcaaaaag gccaaaagga tttaaaggaa tccgtcgaag catagctcca acttctccgg
     1201 atcaaaaagc ggtaggaacc cgtgccgggt tcctctattt gaattttttg gttttgcgct
     1261 acatttttac cattgcagac gccgactagc tacgaaatag aatgagcctc caggctgcgc
     1321 ctactcattc tatttctcgc aagtggcttc gccaggcggc aagggcaagc aatgtgctac
     1381 gcacccttgc cgcctggctg ctgcatggta aaagcttcgc aacaaaacgc aaaaaattca
     1441 gcgtttggct actgctgtgt agcgcaaaga ccaaaaggag cgaaaaccat gaaaaacggt
     1501 actatcaaac gaatggcaga cttgaagtct gcactgaggg aagaaaacca gaaaaaacag
     1561 ctctcgattt ttgatgaaac gctcggatat gcggcaattg attttcgtat cgaccaccaa
     1621 caaaaaggtg aacgaagtat ttcgtgggtc acgttgattg cggcaccttc ggtcaaaatg
     1681 aagactgtgg gagagttttt ggaacgatta gaggtctatc ctgaccaaac agtagttgcg
     1741 acagtcggac tggaaggtgg ctactcaccc gtccatttag caatggacgg tctagggaaa
     1801 gaaacatggc tcgttctctg tgatgaaatt gcctaccaca attttttctc accacctgaa
     1861 cactcggaac tggaagttga attcgatttg aaaggggcag tgaaagagca cctaaaatta
     1921 caactagaaa cgtctaaaaa cccaactgaa acaaggcttg cgggttggtt agagcaacaa
     1981 gaagattctg agttattaat tggtgtctta aaagaagaaa agtccctatc cgaattggtt
     2041 caattttgta ttcagaaagc ttcagcaagc aaaggaaagc agtccgctat gatggtaagt
     2101 gatgaggttg tttttcagtg ggtaagggaa tattacacga ctgatattga aatgaaaaat
     2161 tcaagttcag tgaagggaac catgcagacc acaccaacac ctcaagtgat tgaaacagaa
     2221 cgaaaggagg aagaacagca gattagtctg ttctaactac tatgaaaatc gcatacgatt
     2281 atccatttaa accactcacg ccaccgaaag ggtttttcaa gtggtgtagt tcgcaaatta
     2341 cgggctatcg ctgggcgaac aaagaacgga caattgtttc gacaacacga ccagaagccc
     2401 cgattatttc caagcgattg acaaaagctt caaatttgaa tagatcagaa cgctgttacc
     2461 cctttgcgct cgtgttaagt agcaaaactc gtgttgaaat tcaatctttc ggcatttggg
     2521 taggaattga gaatggtgtc gaaacctatg aaattgctcg aacgaacctt gaatgtttct
     2581 gcaatgatgt acattatcag caacattatt ttagaaataa gtggtatgga gggttgctga
     2641 atcatggtta catgactggc tcatacacga atacgcagtt ctatcccaac aagtggcgag
     2701 aaaaactggc aaaaaattca ccgctaaaat attgtaatct caaagatatt tcttggtacc
     2761 atctcgccca cgcctataaa tatcggcggg agattgagtt cttaaaactc attcatgcgg
     2821 ataaatttgc ggacgacatg ttattttcgg ctatgcacgt tatacgggat agggtacgag
     2881 ggaaaagtat cgactatcga acggttaaca tgaaatggct tcggaaacac aaaggaattt
     2941 tgaaaaactc tacaattacg ttctgtgaaa tgcgattaaa ggaagctatc agaaagtgtc
     3001 atggaaaacc aatagctgga atcgaagaaa ttctgacggt ggaagacttg aaacgtatac
     3061 caacatgcgt tgggctaacc agctttcaaa aatgggccgt tgcacaagac cttgactttg
     3121 gcatgtatgt ggactacctc aacatgttag acgaactcgg aatcggtgta gataaggaaa
     3181 acgccatgcc aaaggaattc agagcagctc atgatgaaaa agcaaaactg gtaggcgtta
     3241 aagaaaataa agaatatgaa aaagaattta atgagcgagt attgcaattg aaagggcttg
     3301 aaaaacgtct aggtgatgtt caaattgttg caccaaaaga aatgggcgag atgttggtgg
     3361 aagggcagaa ccttcgccat tgtgtcggtg gtcagtatta tattagaaga catgcgaagg
     3421 gagaaacaac gattctcttt gttcggaaag cggacaagtt agatgaaccc tatctcacct
     3481 tagaaatgag gaatcaccgt attgttcaat tgcgtgggaa gagaaaccaa gcggcacctc
     3541 ctgaaatcga acagataata gaaaaatggg ttaaaaccgc aaactgataa gacgtttacc
     3601 agagtgatga aaagcacagc attctggtaa gccgcttatc gggtaaaact ggcgcttcgc
     3661 ctgcggtgca gttgcccttg gctcaaaacg cgacagtctt cttttgccta gtgacagaat
     3721 ctaaccgctt gatggctacg tgccttcgcc agtcgcagtt gctttggttg gctgcgttcc
     3781 tccgccaagt cagccgattc agtcattttt gccagggcaa aaaagcaaaa ggattgaaag
     3841 gtcttgctcg aagcatagct ccgacttctc gcaagacggc ggggaaatca cccgcctgaa
     3901 aattccgagc gaattttgtt ttgttcgtca atcgccgctc ctattccggt ttcattttgt
     3961 gtgcaaaggt tgtttgctgt ttgtggttat agatcaaacc cggtggcagg taaagggtat
     4021 acaagcagca aaatccgccg ccctttacct gccaccgggc ccgatctaga gcataaccac
     4081 agcaaacgac ctaagcacac tggaaaaaat gaacccatga caaataggaa caaagggcac
     4141 gactggacaa accaaaattg gaatttcttt aaagattcca gctccgccct agctcctaaa
     4201 aaaatgggtt tcaaaccaac aaaaaaggag ttttttacat ggatgcttta agaaaattgg
     4261 aagtcacact ttacaacaaa caatggttta cactgattgg gattgaggct tgtttgaaaa
     4321 atcaggatgg tttttccgct gaagaggttt tggaccaaat acagggttgt ctggaagagt
     4381 acaaacatgt tgagggggca atgatggaag aatatcagca acaaaatttt gctaagttgg
     4441 aaaccgtgtg ataccagctg aacaatcgga aatgcaaggc atgtaattcg gatgacgacc
     4501 tggtccttgc caggttgttt tccggaatca ttgaaagggt gttgaattat gcgaaaaact
     4561 atttcattaa catcgaacaa tatcaaggta agcgggcata taggtacgtg gtatgtatac
     4621 gccagtcgag tgtatcacgg acgtcgttta tttcttgtag aacatgaaac ctacggggat
     4681 cacgctgcca acttgatact ggataaaacg ggaaactgtg tcatggaaga tgtttggaat
     4741 gggtgggaag attatgaggt gtatattgaa agctgaaggg aaagagtagg caccagtctg
     4801 atcttaggat gacctaagct gagagtcttc agcttgggta tgttcttgta ggctaattct
     4861 tggtaaaaaa gaagagcagc attgtttatt tagcatgata tactatttgt gaaaacgggg
     4921 ggatttgtga tgatttgtcg atattgtaat aaaaaggttt tgaagctaga agggggccag
     4981 tatcactgtg ccaattgtga gctggatttt tttgagaacc aactgattga gttcaggtct
     5041 ggattgtatc gagaaccgac cgtcgaaaca gatccgttct ttttccaata cacgacgaca
     5101 aaagaattat tgcagatgac ctatgtagaa ttgctgtctc ggctgaagct ggcgagaggc
     5161 gtctatcgtg agttgaggaa aaaagtttat ggccacaata gccaagcaga tgatctggat
     5221 cgcgagaacc tgaaggcagt cacttcagca aagcttgtca tcgaaaatat tatgattgaa
     5281 aaacatggct attatcccgc cagcgttttt gagtctgtta tagataaaga agttttgaaa
     5341 aataaaaaac aaatgcgaga ggtggaaaaa aagcatggtt aaatttgagg atcaaccgaa
     5401 gcaggggtat acaggtatcc ggacgctaga aacgattgcg agtgtatttg aggatattca
     5461 ggccagcggt gacatgggac agggcgagaa gaatttgcga tacttgcagc tgatgaatga
     5521 gttggaagag gaccacggag ctatgattgt gaatccgact aaaacggatc tgaatttgcc
     5581 tgcggtcgca ttataccgga aggtttctca agctcgagta ttataggcgg aacttgagag
     5641 gagtttacga atggataaat cgaaagaaat catctttatg ccagactatt tagtaaaaga
     5701 tcatacggtg ctagttttag attcagcaaa caaccttcac ggttttttga gtatagaatc
     5761 aaccgacgat cattttgttt ttacaaatga tatagacctg ctcaaaaccg tcccgaaaat
     5821 tattgcggaa gaacttgcaa ctgatattcg cgcttctgaa ggcgactata ttccagtaac
     5881 aaataaagat actggcgaag tatctgaatt gggttcagac gacctccaaa tcgtattttc
     5941 tgcggtagag tggtgctaaa ggtagcggct gagtctaacg atcatgatga atgcatggat
     6001 tcaactaata ggagaaaaga aggtagaaat taatgaacga gccggatatg acgaatcagt
     6061 ttcgagttgg ggaagtcatt cttcagcgaa tgattgatcg gaaagaattc gaagacattt
     6121 atcgctcagt gacccgaaag aacacggtgg acgcctacta tttcgatgca gggcgtagaa
     6181 tcgtaacagt atttgcttat tacttgggaa gtatctctgg ttcttcagtc gatgaggaaa
     6241 gaatcattgg ggagcttatg gctttgatgg caacaatgga gcgcgacagt attcctggtg
     6301 ttgttgtttg ggataaaatt tcaaaacttc caggcacacc ggaacattcc atgcagcttg
     6361 cttcagaaaa agcaatcgaa gacaccttta cttttttact tgcagctgca aaggctgaag
     6421 agccgtatta tctcaatggt gggacggaaa ctatcgtatc aatgattgct gctgaagtag
     6481 aacaggattg taggtagcag caggaggcag aaaatatgtt gaaatgctat ggtgaagctg
     6541 agctactaga aatgcttaag agaaacgagt ttgaagagga ttcaccgatt cggagtgttg
     6601 aagatctgct tggacacctg gatcaggcca gtccaatgat gtgggtgacg gtgtatgtac
     6661 cagaagttca aagtgtgtac ggtattcatc gagagacgtt gatttctacg ggacaaagcc
     6721 ataggagtac atctaagcca gttgctttgt tagaggcgac aggttatcaa acaagtgaac
     6781 atgtctttgt ttctgaatta gcggcgcttt tacagatgga ggtagtcccc aagaatcgag
     6841 aaatctttga attacagctg tttgagcgaa aacgggggaa gtacatgttg cgtcgctggc
     6901 aaatgattgg ggatcagcca gtactaattg cccaacgtgg ctaattatta tttaaagatg
     6961 agtgttgtgt aagctgaggt gacctttatt ggatgaaaag caacaaaaac gacaactact
     7021 aattaaatta gaatcagtcc taaaatgaat gactatttta ggactgattt tgctacataa
     7081 aaaacagcta gcagattact agctgttctt tctttattca aaagtttcga tggaagtcgt
     7141 catttgatcc ggttttaagg tgatcttacg gtagccccgt ggcaaatcgc cataacagtt
     7201 ataaccggtg acatttccgt aaatcaatcg ttggcccaga tagatgcctt cgaaattatt
     7261 atcatgatca tgaccgcaaa aagtgccgag taaatggttg ttttctagta aatggctgaa
     7321 taagccggta ttaagctttg gtgccgaaat tcgcggattc atttcccaaa aacgcccctc
     7381 tacgatccgt tcgccggctt gtagatattc aggtaatgga atgtggagga acaatagatc
     7441 ttttgtatga ttaatctttt gatatttcgc gaaagtttcg tcgtaccact tgatttgttc
     7501 taaagaaacc cagtcgtagc tctcataatc aattaaggca ttggcgccgg aatcgataaa
     7561 ataaagaaca ttaactaatt gattatcatc tttaatttca attgtaaaac attctttttg
     7621 atttggatca aagaattgat tggcttttaa agccagatga ttgaagagtt ttttctctaa
     7681 ttcgtggaga tcgtgacgat caatggtttc ttcggagtcg tgattgccgt aagtgattgc
     7741 caatggaatc gggtatttat tgaaaatttc ggctaaagct tctaaccctt ttgtaggttc
     7801 aataacacca tctgaccaaa ttaagtcgcc ggttatcacg atcaaatcag cggttgtctt
     7861 cgctaaggtt tgatcgatca tggcaaaagt tttttgatct tcttcattaa aaggagcttg
     7921 accgatgtgg atatcggtaa attgcaatag ttcaagtttg tgatttttta aagtaatcaa
     7981 gggtaacatc ctttcttaat acgttattaa aataaaccct atcaaaaaat aatgcaaacg
     8041 ctttaataac tttttttaag attaaaaaaa taaaatatgt cattcaagac gttgaaagcg
     8101 attttagaat ttgctattct ttgggtgtgt tgaagagagc aaaaagaaag gggcttcaga
     8161 atgaaacaag attcttattc agttaaggat gcaaacgtta ttttatcaaa attgatcttg
     8221 ggttccagtg attatttgaa gttggacaac aaggacaagg tcgataggat gttcactaga
     8281 tacgtagaag aaggtggacg aacttttgac actgccagac attaccgtga aagtgaggcg
     8341 gtgattggca gttggttaaa ggataaaaaa cgtgaggatt acactattat cactaaaggt
     8401 tgtcatccag tgcgggaagc accagatacg ccgcgtgtta atccagaaag tatcttagca
     8461 gacttgacta ctagtttaga aatgttggca actgattatg tcgatgtgct tttactgcac
     8521 cgagatgatc ctagccagcc ggtcggtcca ttaatggaaa ccttaagcca attggttgat
     8581 gaaaagaaaa tccgatcttt tggtgtttct aactgggagt taccacgaat tattgaggca
     8641 aaaaaatatt gtgaagataa cggattaaat cctttgtcat tcaatagtcc aaattttagt
     8701 ttagccaaag ttaatgagcc tcgttgggaa aactgtgtga cagcagacga tctgatgatt
     8761 gcttggcatg aagaaactga ttttcctttg atttcttggt cggcacaagc agaagcattc
     8821 tttggaaatc gcttctcacc ggatgatgtt aacgatccgg agatggcaga gtttgttgct
     8881 gtttatttta atgacttgaa ttggaagcgc ttagtgattt gtgaagagtt ggcaaaagaa
     8941 aaaggtgtca agccaatcca aatttctctg gcctacgtgt taagtcaaac tttcccctca
     9001 tatgccacaa ttggtccgga ggaagaatgg cagttacttg attcagttga agcgatgaat
     9061 attcaattat ctgctgaaga aatcatgcgt ctgaagaaag gagaataagc cagttggata
     9121 aaacaaagat tgcggcccaa atgtattccg tccgtaaaga actggaaaaa gatccagaaa
     9181 atacttttaa atcattgaag gaaattggtt ttgaagccgt tcagcttgat ggtatgcggg
     9241 ggcacaaaga aactgaaatc aaagcattag ttgataagta tggtttcaaa attgccggga
     9301 tgcacattaa acatgatcgt tttttcaatg atcgagacgg aattatttct gaatcattat
     9361 tgtttggttg taaaacaatc tacgataaat atatcgatga tgaagagcaa catacgaaag
     9421 gctatatcca aacgaaacaa caattggtca cgactcaagc gactcttgga aaactcggtt
     9481 ttagaatcgg ccttcactgt ccggaatatg attttaatca aacagtcgaa gggcggacca
     9541 ttttggatta tattacagac ccagtttttg ggacggcggt ctatgcggag ccagatactt
     9601 attggatctc ggtagctgga aaagatcccc tagaagaatt tagaaaattc tctggtcggg
     9661 caccgattgt tcatttgaaa gattacatca aaggttttgc accggaagat atggataata
     9721 acttgacgga attaggtaac ggagacgtcg attttaaagc cattgttgaa tggggcgaaa
     9781 aaagtggtgt tgagtactac tgtatcgaac aagattattc caaaatcggt atttttaaca
     9841 gtttagcgaa aagttttgat cacttaatgt caatttaatc ttaggagcga gaagatgaag
     9901 aaaaatagtc gtattgtcat gatgggtgtc gcattggtaa gtggtttggt attagcaggt
     9961 tgtggaaatg gcagtgcctc aaatagtgat aaagtggaat tatctatcaa ctattacaat
    10021 ggtgcgatca gtaaaaaaac catggataat gctaaaaaag aattttctga ctacaaattg
    10081 agttttaaac aaattcctgc caatgatgat tttgatacaa agctaaaagc cagcttaaat
    10141 tcaaaatcgg caccagatat cacagcaatc aacagtaata tttctgatta cctgccatat
    10201 gctgataaat tcgtaaacct attggattac gatagtgaaa cattagccgc tgactttgtt
    10261 gaatggaagt gggattcttg ttttacaaat gacaaaaaaa cgcagattgc catgccaatt
    10321 gatattggcc caacagcttt attctataat gtagacgctt tcgcaaaagc tggtttgcca
    10381 acgaacgatc aagaaatcag taaagcattg aaagatgagg aaagcttgat ggctgctgcc
    10441 aaacaaatga aagaaaaagc cgatatgcct ttcttccaat cttctctttc attgttgcaa
    10501 tctaaaaatc gcacgatgac tgaacatatt tacgatgaaa atggcaaatt gacctacgct
    10561 aacggtcaat taaaagaaat ctgggattac gctgttaaag cagaaaaaag cggttacact
    10621 ttgggaacaa aaggaaatag tgctgatggc gccaactccg ttcaaaaagg catgtttggt
    10681 ggcatgatcg aagcttcttg ggggatcaat gacttagctg aaaatggcgt ttcaccaaaa
    10741 caatggatga tcgcaaaatc tccatttacc gcgtctaact acggtggttc atatttggcg
    10801 gtgatcaaaa cttcaagcca tccaaaagaa gctgcggaag ttgtgaaata tttgacgaat
    10861 gaagacagcc aacgggctaa ctacgaagaa ttggctttat tcccatcaaa tactaaagtt
    10921 tatgatgatt ccttcaaagc agcgaaaaat gaattattcg gtgatgaaga atttaatcac
    10981 tactttatcg atgccgctaa cgaattggaa tatgtaccat cagatccacg tgaaagtgct
    11041 gctttcaaat gtttcgaaga ccaaatcatt ttggtagctg aacaaggcaa agatcctgaa
    11101 aaagcttgga acgatgccgt gaagaaagta gaagagatgt ctaaataatc agaatgtgat
    11161 ttcgggatat gacatcgatc aaattttaaa aattggtggg tgtcatagac ccgtaaatgg
    11221 aggtctaagg atggagattg gtgagatgat gaattcagaa tcgaaatcaa aaaagaaatt
    11281 tagttttgat aaagttgccc cttacgtttt tgtatccccg ttttatattt tgtttttgat
    11341 ctttggtctg tttccagtta ttttctcagg cgtcctggcc tttagtaaat gggatggctt
    11401 aggggatatc caatttgtcg gtttggataa ttttacccgt ttattgcggg atgctgactt
    11461 ttatttatca gtcaaaaata cgctaatcat ctgggcagtt gggacattcc cgatgttgtt
    11521 tttggcacta atattagctt tcttagtaaa tttgaaattt atcaaacata aagagttact
    11581 aaaatcgata tttttcttac caaatatcac ttctgttgta gcggtgacaa ttttatttgg
    11641 cttgattttc tcaaatctgg atggcttagc caatgcgcta ttacagtttt taggatttga
    11701 accaattaaa tgggttacgt caccattttg ggttcgaatc gtgattgctt tgatcaatat
    11761 ttggatgtac ttaggctata acatgatcat ttatctaaca gggattacaa aaattccaaa
    11821 tgattactat gaagctgccg ttattgatgg cgcaaccaat atgcagattt tttggaacat
    11881 tacgatacct caattaaaac cgattatctt gtttactgtg atgatgtcaa ccattggtgg
    11941 tttgcaagtc ttctctgaag cacaagtatt agtaccccag ggggcgactc ctgaaggtgg
    12001 ggcgatgacc atcgtttact acttgtataa tacggcgttt acaaacaatg agtacggata
    12061 tggatcagca gttgcttggg cattagtctt gatcattctg gtcttttcgt tgatcaactg
    12121 gtacttgacg acttatcgaa tcaaggggga ataaacatga gcaatcggat ttttaaaata
    12181 cttgttagag ccttcttagc gattggtgtc tttctttcga tcttcccatt ttattggatg
    12241 attgtaatgg ccagtaaaga taatagtgaa atctatcata ttccaccaga tttgatgcta
    12301 gggtctgaac tgatgaataa tatcaaaacg gtttttactg aaacaacatt ctttagctcg
    12361 ttgcttaata ctttatttgt ggcactaact tcgacactgc tgattttgtt ctttgattcc
    12421 ttagcgggtt tttcatttgc caagctgaac tttaaaggga aggcctttct ctttactttc
    12481 ttattgggaa ccatgatgat tccgggtcag ctgaatatta ttccttcatt ttatttgatg
    12541 gataaatttg gttgggttgg ttcttacaag gcgttgatta ttccagggat ggcaaatgcc
    12601 tttggtattt tctggatcaa gcagtattgt accgatgcca tccccgacag tttgttggaa
    12661 tcagcaaaat tagatggttg tacacttttt aaagcctatt acaaagttgt attgccaatt
    12721 atgaaaccag ctttagcatt tttagcactc tactctttta tgggtgcgtg gaatgactat
    12781 atgtggccat tgattatctt aaacgatgaa tcaaaataca cgttgatgtt ggctttgaca
    12841 caactacaag gactttataa aacaaattat ccattggtaa ttactggtac gttactggca
    12901 actttgccac tactcttagt ctttactatt ttcagtaagc aattgatttc tggtatcaca
    12961 gaaggtgccg ttaagcaata gaaagaggta ttagtgtgaa aacaaaacca cttagtatcg
    13021 gagatacacc caagaataga gcgcatggca aataaacgtc aaaaaccgca ggtctgacga
    13081 ttatgaaagt tatttagatg gctggctcta agtcttttct aacaaaatca tctggcaata
    13141 aaccttctga aatcagtaat ttggtcgctt gtttaattgc tttatctttc tgtttttgaa
    13201 caagattgtc tggcatatca atcttatata agtcagtcgg attccaaacc tcaccagttg
    13261 aaagcatgtg gtagatagct gtgagcatca ttcgagcaat cgtgatgata gcccgtttct
    13321 taccacgtcg tttgtaaatg cgcacgtact tcaaacggta gtacggattc ttttctttca
    13381 ccaaaacggc tgcgtgtgta catcaatacc acaacaaacg ggataaacga ctttcatgat
    13441 tcttcgcccc tttctaaaag tagcggagag gcagtgactg gtctgggcag catttaacag
    13501 gatttgttaa aacaatgatt agtgtgcgga cttctatagt tccgctcatt tgtgcttgaa
    13561 cgccccgacc ttacactggt taatatgctg gttcaaacaa tcgaggtttc ctacaactca
    13621 cctcaccgtg ctttgtagta tgcctctcct aattctatta taaacggaaa tccgcgtctt
    13681 cattactatt tgactcgagg agcggagcga gtcgagatgg aggttaatat gaaggaaaaa
    13741 tttatcgcct tacaagcaaa attaatggac ttgattttat tgagtggttt gatcttgatc
    13801 ggggcaatga cgataattgg tttcttttat ggaatcgttg cggcgtttgt ttatctagaa
    13861 actaaaagtg aagataaatg gcaagtttta aaaaaacgtc agacaatctt gattagtctc
    13921 ttattagaaa tgtcagcagt ttttgtagcc ggtatctttt tcctaaatgc gcgtttgctt
    13981 tcgcagttgc aaggcggtgc aacaattatc attagtttct ttttagccgt ttgtaccttg
    14041 atcttaattc cactgtattt cattttattg cacaagctag cagttggaac aatttttacg
    14101 aaggaaacat ttcttaatag tgtcaaggtc atcttaatcg gttttcctac ctggtttacc
    14161 caaattatct taatgatcgc gctgagtgtt ttgacttatc tgtttccggc tttatcgatc
    14221 attacagttg ggtacttgat tacccgcaca aataagatca ttaatgtgca aaaaatgaaa
    14281 ttagctagtt tgcaggaggt ctattaatga aattgagtca acgggaaatt gatcttttaa
    14341 agctggcttt agatggtgaa gaagaaacga ctgtgccgca actaaaaaaa caattgaagc
    14401 tctcagtcca tcaaattcgc tatgctattg ataaagtgaa ccgcttttta attgaaaaaa
    14461 agttgaatca aatttttatt gaaaaagatc ggctggtcat tgaacaacga cgaaaaatca
    14521 aaaaagaaat tgattttttt atatctcact cgacgccaga gcagtttaaa tttaccgcag
    14581 agcaaattga atattttatc tcattgaaac tgctgctaag tgatcaacgt ttgccaatca
    14641 attattttgt agaaacatta tcgacttccc ggacttctgt ggtaaatgtt ttggaccgaa
    14701 tcacacagga actggctgaa gaagaaatcg aattatcgaa tgtaccacga aaaggttaca
    14761 tgattgagga gtatagtttt aaacgatttt cctatttcat cacgattttg aaaaaaatga
    14821 tcaatattcg tgaaatctat agtttctact atcaagataa tttgtattca aaagctggcg
    14881 acttagtatt ttttgacctc tttgatttgg atatcctctt tgattcttta ggggagacga
    14941 ttcagtattc aaaacagatg gccagtgaaa ttgatgatac gaacttttta tttttaacga
    15001 ttttgaacta taagtatcgt gagttgatca atgcctttga gatgactgat cgaacggaaa
    15061 aattacaaat gatttcaagt tttattagtg aaatcaaaca aaataatccg aatcaaatgg
    15121 aaaacaacaa tctagccaag cgattgattc gctatatcaa ccgtgaatta gccgatgttt
    15181 ttgaagtaaa ggattttaaa gtggcctcta acgcactact atatcatatt caacgcttta
    15241 tttttagaca ggaaaatggc attccttttc gcgatattga tagcgaagtt ctagtaaaag
    15301 accacaagaa tttgtattac gtgatttttg cggcaatccg ttcttttcaa aatgggttgt
    15361 ttgaaagtgt agaagcacct gaggcggtat tgatcacatt gtattacatc aatgaaattg
    15421 aaaagaatgc accaaaagtt tttaaagcgc cgcgaattct gattatttgt ggtgaaggcc
    15481 gtgcaatttc cagcgtgtta aaaatcaaaa tcagtaattt gatcgattcg aagtggatcg
    15541 atacgatttc agtttttgaa tttgagacac ccttgatcga tcattatgat ttgatcatta
    15601 ctacggttcg gttgccggaa gtcgagtcag acaaagttct atactcggac aatgtcttct
    15661 ccgaaagttt ctttgaacaa attaaattga aactatcggt aggtgggtac tcatttgcga
    15721 cccaacgcct tggcaagctt tcacaaatca tgaatgcaat cgtcgaagag gttaacgatg
    15781 atatcaatat gaatcgtctt gaagcaaaga ttatcaaaat tttggcgaat aatttgctgc
    15841 aaaataatcg tcccaatgaa caaaaattta tctttgacgc cagcatgttg aaatatcatc
    15901 aagcagcgca aacatggcaa aatgcgattc gcattagcgg gcaggaactt ttggcaaagc
    15961 actcgattga agaacaatat gttaataata ttatcagcaa tattgaaaat tttggacctt
    16021 atatgatcat tgcaccgggt gtcttgctgg ctcatgctgg tgaacaagat ggcgtgctta
    16081 ataatggctt tagtatgcat gtttttcatg aaccgattac ttttccaggt gaaaatgttt
    16141 ttccggtaag tgtgatcatg acaattgccg taaaagatcg cacatcttat cacgttgtcg
    16201 aaaggatcgt gcaatgggtc ttagatgaag aaacgatttt ttcgttagct gatgagaaaa
    16261 acagacaacg gatcgaacaa atgatccgaa atattttgat gatgcaagag gtgtaacatg
    16321 acaatgacaa cggatgaaat atctttcgcc ttgatttcta aggctggtac agctttttca
    16381 atgatgatcg aggcattggg agatgcccgc gaaaaggatt ttgaaaaagc agaagaaaag
    16441 aaacaagaag ccgaggcttt gatgaatgaa gcccataatt tacaaacgaa tttgatcgta
    16501 aatgaagtca atggcgatga taatcaaatc aatgccttat tgattcacgc ccaagacaca
    16561 ttaatgaata caatcttgat gtcgacgatt gtcgatgaat ttcttaattt atataaggca
    16621 ggagaataaa atggagaaat tatcaatttg cttagcgtgt aatttagggt catccaccag
    16681 tatcttggtg aataaaatga aacaagtggc agccgggagt caaaaattgt cttcttttga
    16741 tttaatgatt gacgccattc cagcaggaaa tatcagcacg gaacaagtga aggattatca
    16801 aattattatg ttgggaccac aaatcggaca caggcgcaat gaaattgagc aaaaagtagc
    16861 tagtatcaat attccggtaa cagtcatcga tgcagctgac tatggcagta tgaatgccgc
    16921 taatattttg aaagaagcaa tttatcaaat caaaactgct aattaaggat ggatagccgt
    16981 gactttagat tatatcaatc aggtagttgg aaaaatccaa acaaaaatga ttccgacttt
    17041 aggtaaaatc gaaggaaatc aagtttttaa aagtatgaaa aaaacttttt tgtaccttaa
    17101 ttatgtctta ttgatttcct cggttttggc tattttgaaa cttgtaaatg aaaagtttat
    17161 ccaccaacaa cagattgctg atttgacgtc taagttgttg ttattactga tggataattt
    17221 tggctggttt tttctaggga ttctttgctt tttgatgttt caggaaaaag gaaagtttca
    17281 ctactatcta tgcagtgcgg ctttgtatct catgttttca gcaaaagatc tgaatttatt
    17341 atcagcctct aaaattgaaa cactattttt agcattgttg gctctgttag ttagctttgt
    17401 tctggtgagt ggttatcaat tagtgctgcg gggaattaaa aagatcatca agacaccaat
    17461 ggaattttta gataatttgt tactgatgat ttatttcagc gtgattttca tggttatcta
    17521 tcaaatattg tctcagctta aaggaattca tttagaaaat attttgagtt ggttaaacat
    17581 tgataaccca atgatggttt ttgtcattgt ttttcttgaa atgtttcttt ggtacatggg
    17641 catcaatggc tatggcatat tagcgccaat tgttttacta tttgccatta gtaatttgaa
    17701 tgcaaacttt caagcaatcg ccacgggcga ggcaccgcag tttattttca cgcctaattt
    17761 ttgggattat tttctaagtg ttactggatc aggaattact ggcgccattg tgattttatc
    17821 tttgttgagt aagaaaacaa cattaaagaa tctcggtcga acagccgcta gtggaacctt
    17881 gttttcagtt tccgaaccaa ttgtttttgg gataccggtg gtgatgaatc catacttttt
    17941 tatccccttt gtgattggga ctccgatttt aggggttatt caatggtatg ttttcaaact
    18001 tggttgggtt tcattaccga cttattttgt ggcagattta ccactaccat tttcgagctt
    18061 cttggcaacg atggattggc gttctttgat tttgaccgcc gcaaccattg ccttagcaac
    18121 tttgatgtac tggccgtttt ataaagcatt tgagaaaagt tatgtagaaa aaaataatga
    18181 tgataagtat caggatctag atttggattt ttagaaaaga ggaataaaat tgctaaaatc
    18241 tttttttaaa atattttgcg gaaactttat tttgggctta gcatacgcca aactgatggt
    18301 tccaaatgag atcattaatg gtggtgtgac gagtctttcg ttgatattta gtaatctgac
    18361 ttccctcaat atcaccttat ttacaaatgt catcaccgtg acattactgg cagtttcatt
    18421 gctcttttta gggaaatttt tctttttctc ttccgtcgtt agcagtgttt cttatttgct
    18481 gtttttcaat cttttctacc gcttgccatt ctcactacat acaaatgttg tggttgattt
    18541 tcttttggca gtattgctga ttgccatcgg ttattattgc tgcttgtcag aaaattcttc
    18601 tacagcaggt ttagatgtat ttgcggttat tttgaataaa aaaatcccca aaatctcagt
    18661 ggctgttttc ttgaggtata ttaatatcgc agttttagtt ttggggctgt tttcatatgg
    18721 aatcaaggcc gtcattttag gggttttatt tagttttagt tttactaaag taatggatct
    18781 tttgatggaa aaaggtgcgg atccccgcaa attttttcac a
//
LOCUS       DAJSMI010000040        18638 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJSMI010000040 DAJSMI010000000
VERSION     DAJSMI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.040"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(335..1627)
                     /locus_tag="ON430_001122"
     CDS             complement(335..1627)
                     /locus_tag="ON430_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3219049.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1624..2247)
                     /locus_tag="ON430_001123"
     CDS             complement(1624..2247)
                     /locus_tag="ON430_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219050.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2250..3086)
                     /locus_tag="ON430_001124"
     CDS             complement(2250..3086)
                     /locus_tag="ON430_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3219051.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(3103..3990)
                     /locus_tag="ON430_001125"
     CDS             complement(3103..3990)
                     /locus_tag="ON430_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3219052.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(4006..5289)
                     /locus_tag="ON430_001126"
     CDS             complement(4006..5289)
                     /locus_tag="ON430_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3219053.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(5972..6655)
                     /locus_tag="ON430_001127"
     CDS             complement(5972..6655)
                     /locus_tag="ON430_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3219054.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6673..7290)
                     /locus_tag="ON430_001128"
     CDS             complement(6673..7290)
                     /locus_tag="ON430_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3219055.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7300..8124)
                     /locus_tag="ON430_001129"
     CDS             complement(7300..8124)
                     /locus_tag="ON430_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR3219056.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(8117..8677)
                     /locus_tag="ON430_001130"
     CDS             complement(8117..8677)
                     /locus_tag="ON430_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219057.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8851..9807
                     /locus_tag="ON430_001131"
     CDS             8851..9807
                     /locus_tag="ON430_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3219058.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(10112..11068)
                     /locus_tag="ON430_001132"
     CDS             complement(10112..11068)
                     /locus_tag="ON430_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCR3219059.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11172..11774
                     /locus_tag="ON430_001133"
     CDS             11172..11774
                     /locus_tag="ON430_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3219060.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11862..13361)
                     /locus_tag="ON430_001134"
     CDS             complement(11862..13361)
                     /locus_tag="ON430_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR3219061.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13377..13901)
                     /locus_tag="ON430_001135"
     CDS             complement(13377..13901)
                     /locus_tag="ON430_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR3219062.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14199..14948
                     /locus_tag="ON430_001136"
     CDS             14199..14948
                     /locus_tag="ON430_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR3219063.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14939..15724
                     /locus_tag="ON430_001137"
     CDS             14939..15724
                     /locus_tag="ON430_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCR3219064.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15693..16409
                     /locus_tag="ON430_001138"
     CDS             15693..16409
                     /locus_tag="ON430_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3219065.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(16511..17545)
                     /locus_tag="ON430_001139"
     CDS             complement(16511..17545)
                     /locus_tag="ON430_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCR3219066.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(17549..18607)
                     /locus_tag="ON430_001140"
     CDS             complement(17549..18607)
                     /locus_tag="ON430_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCR3219067.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         5577 a         3925 c         3271 g         5865 t
ORIGIN      
        1 actgttttgc acgtgaaggc agtttgccat atttgaataa aaaatgttgt ttcaaatatt
       61 gaaaacagtc taacactact ttctgaaact gacaacgtgg tttacttatt tttttgtaaa
      121 atctaatgat ttttaatgca ttattccttt ttgttcgggt ttaaatagtt atttttattt
      181 tctcctatct aaaaaagcag cccacataaa aagaaggcat gacaaaaagc atgtcatagc
      241 ccctttttcc gaataaatag tgttcaaaag acagcctatc ggcaataagg caagatttta
      301 taaactacgg gaagtaattt ccgtaaatct attcttattc ttttaagctg acaagctcgt
      361 caatcactac tgcttttcct gaagcaatag aatgatttgc tgcgattcct gtcaaaatcg
      421 acaaaatacc atctacgtga gttgctgccc gattccacgg atcttcttct ttttcttcac
      481 caaatagatc atctaataaa cgcggatctc ctccgccatg tccgcccgct tcttgttcga
      541 cgattacctc atatggctca ccgaacatcg gatagacatg gatggactga agaacggctg
      601 ctccttcatc ctttttatct ccacctgcat tgatgtaaga cttttcgatc actttcattt
      661 cgatccgtcc tttgctgcca ttgaaagaaa cattgaatcc ttcccacggc atatacgcgt
      721 ttagcgaata cgtcaaaatc gcaccagttt catatttgac cgttacgcct agtgtatctt
      781 caatagagat atcatcggaa aatacactgc ggtcacgaat atatccactg tctttttctg
      841 cttctaaata catttctcgc aattcctcat tggctgacag gtctatagca aatggatcag
      901 ctttagctag ttcgcttcca tagcatcgac tgtaaaatat ttctactccg cgtttctccg
      961 cattatgcat cccataaaag tttaatccac cgaatgcaaa taccttttgg ggacggctgt
     1021 tcaaccaaaa attcactaga tcaaaatgat gggtagattt atgaacaaga agcccgccgc
     1081 tattgtgctt atttcgatgc catcttctga aataatctgc cccgtgttgc gtatttaaca
     1141 gccattcgaa gtggatagaa aacacatcac caatcacatc actttctatc agttccttta
     1201 tcttcgtgtg atacggtgca taacgataat taaaagtgac tcgcaggctt cttcccgtct
     1261 gtttttggac cgttattatt tcttgagctt tttcctcatc gatagtcaga ggtttttcgc
     1321 aaataacatc atatcctttt ttcattgcac gaatgatata atcatgatgc gttcgatcta
     1381 ctgacgtaat aatgacttca gccggccgta cgtcatccag tagttgctca aattgtgatg
     1441 gcatacatgt ctttactttt ttctgtccat acttgttcac aagtatccga tttgcataat
     1501 ccatccttgt ttgagataca tcacaaaaac cgactagttc acaagtttga tgatacgtag
     1561 aggcaattgc ctcataaaaa aaacgcgctc tcccaccaag tccgacttgt acatattttt
     1621 tcttcattta aagtcattct ccttttcttt taagcaaaat actgtgacaa atggcagccc
     1681 tggatacagt aatgcagtga gtaaaaaatc atgctgtata caccagaaca gtccatacaa
     1741 aaggcataaa taccacagaa accccttgac gttttttgac aacagccaga tattcctttt
     1801 gatcaaataa acaagactat ctgctttatc ttctttgcag atatcaatta gcgtactaaa
     1861 aaaactatgg gatacaaaaa gcaacagaca caatagaaaa ggaatcagat accaagctcc
     1921 aatctttccc actaataaat accaatcaac tgcaaaaata atgacaaaca gctgaaacaa
     1981 cgtaatgatg aaacacatcc gtttgatttt tcctttcctc caacatacct ttttttctgc
     2041 caagtaatgg acagatagat aataagaagg atacatcatc aaaaaataaa tcagatagac
     2101 aacacgagca gacagcacta atcctaaagt gaacaaacat cctagaacta aagcgaaaag
     2161 agcattactg ataagactgg ttaaaaaaat ccgacagaca tagtagttga gcttgtaaag
     2221 aaacatctct tctattggct ttttcatatt cagcccttca gaccgcttgt actgatcccc
     2281 tggacaagat atttattgaa gaataagaaa atcagaaaaa ccggtagcag cgacaacgta
     2341 gacatcgcaa acatactcgc gtaagtcgta ctagatccag cgtctgcaaa taatttgatg
     2401 gcaatcgata ccgtgtaaga tctcggcttg tttaaataaa tcaatggtcc catgaagtcg
     2461 tcccatttcc aataaaactg gatgatgatc gtcgtgatga tcgaaggttt cagcaaagga
     2521 aaaataattt tggtaaagac actatacttg ctgcatccgt caataatcgc tgattcatct
     2581 aactctttgg ggatgctgac catgaattgc atgatctggt aaatgaaaaa tgcttgtcca
     2641 aaaaaatgtg gtaaaatcag cggcacatac gtcccaacca aattcagccg attgtaaata
     2701 ataaactgcg gaatcaaaat cacttgtgca ggaatcatca tcgttactaa catgacgacg
     2761 aaccagaatc tttttccttt gaaatcgatt ctcgataatg cgtaagcaat acaagtcgag
     2821 ctcagaactg tccctatcgt cgaaactgca gagatgacaa acgaattcct aaagaaagta
     2881 aaaaatgaaa ttccagcaaa ccccgcccat ccttttgcgt aattttccca ttgaaaagtc
     2941 tctggaatca aattcagcga accactcata atctcttgat ccgttttaaa agaacctgcg
     3001 atcatccata agaccggata aatcatgact agacctaata gcaaaataaa accgtattga
     3061 aaccatttcg ttactgtatt attcatttct tcctcctctc attcacacaa cgacccattt
     3121 ttttgatgat ttgaaaatca gaaaggtgac agctcccata atcaacagca tgatccacga
     3181 aatagcacta gcatacccca tagaattaaa agaaaaagct tggttataca cataaagagc
     3241 ataaaagttc gtggcatcat tcggaccacc ttgggtaatg ataaatgcct gcgtaaaagt
     3301 cataaacgcc gcgattgtct gcataaccaa gttatacaaa atgatcggcg aaagaagagg
     3361 taacgtgatc ttgtaaaacg tctgccattt gttcgctcca tcgatctctg ccgcttcata
     3421 atacgagcct gggatctctt tgatacctgc agcaaagatg atcattgccg aaccgaattg
     3481 ccaagcagac atcaaaacaa gagggatcat gaccagtttt tgatcgctaa accaggaaag
     3541 cgcaggtgcg cctaatcgtt ccaggaaaat attcactaac ccgtttttag aaaacaactc
     3601 ccgccaaact agagcgatag aaatactgcc accgatcaaa ctaggcacat aatacaaaga
     3661 acgaaagaaa gtcgttcctt tgatattcct cgttaaaaga taagcaatca atagaccaaa
     3721 gatcagtttc acaggtacag atgcaaatac atagagaaaa gtgactcgga tggagttcaa
     3781 aaaacgggca tcactcagca tctttatata attatccaag ccgatccatg agactggatt
     3841 tcccaaacga taatcggtga acgaataata aagtgacaag atcatcggta tcactgttat
     3901 caatagaaat cctaggatga atggagaagc aaatacgtaa ccgaccacat tgtcttgatc
     3961 caagaaccgt tttagttttt cttttttcat gccttcctcc tgctgctatc ctagaatgtt
     4021 tgacgcaaac tcataaagac tctctgctgc ttcttctgct gttacttctt cataaatgac
     4081 tttttcaact aaaagattgt attggtcttg aatttccgga ttcaaagcac tatcaaagac
     4141 attcacttcg ccgttgtcca tatcggaaat catttctaaa taatcaaatg tcgcttgcaa
     4201 agttgcatca tctgcacgag cgagctgttc tcgaatattc gacatgattg gtacaccacg
     4261 ttctcctttt aaaatttcat tggcttcttc actgttttga aagaaatcaa taaacttagc
     4321 agcttcttct ttatgggctg agctttttgc catactcagc atttgcgaag aacgtaaagg
     4381 gataccagat tctccatctg cttttctgcg aggtggatta atcaactgaa tctccatatc
     4441 ttcaggagcc cctgacaata tttctgtcaa ctggttactt gatagaaaca ccattgccgc
     4501 ttctctatcc ctaaccgcct gaaaagacag atccgtcgta cttgcagctc caccacctgg
     4561 ttctggatgg gcccctttgt ctaccagatt tttacgcagc tggaaaaaat ctgttagata
     4621 gtctggattt tcaaatccca agctgttaga tagatcttcc cgattataaa aattcaatcc
     4681 tttctcaact tgtggtattt gaataataca gccagctata aaatcttcga atcttgaaat
     4741 agcatagatt cctaattctt cagtgatttg atagctgatt tgttcaaatt catcccatgt
     4801 ccaatttgaa gctggttcag ggatatttaa ttgttcaaat atatttttat tgtaagcaat
     4861 cgcaaacgaa ttcaccccgt tagacagtcc aactaattgc ccgtcgtaag tcgtcgttgc
     4921 taaataggat tcatctgcat tagaaatatc aattatccct tgctccacat attcatctaa
     4981 tggttcaatc tgattgatgt attgaggaaa ttgattgttc agttggaaaa tgtcccagat
     5041 atcatctgat gctgctagtg tacctaattt tgtgtaatat ccttcataat cataaaattc
     5101 ataatcgatc gtcacatggg gatttttttc ttcataaagc tcgattactt ttactgttgc
     5161 atcgtgtcgg acttgcgaac cccaccaagc catgttcagc gtgatttttt tgtcatcttc
     5221 ttgcccacaa gccgtcaaga agaataacat aacacttaaa gctaagatga ttctcattct
     5281 tttcttcatt ctctgcacct ctacttccat tgttattttt caatatactg tatttttata
     5341 cagaacatat ttattaaacg atctttttca aggggaataa aaaccaagac ttgccttatc
     5401 gttctgccta ggatgaacaa ctcaatactt tcaagaaaaa ttagatagtg ctgattcttc
     5461 tttcatcgct aaaaacactt cttgcttcaa tgatgggagg ataattacgc aaagaaaaaa
     5521 ggatatcaga caactgccca atatccttga catagctaaa taagcatgct tcttcgtgtg
     5581 cgctcttccc taaaaaacag aaaaataact agtaattctg tatgaaatta ggtacaatat
     5641 acacgtcgat gcacaagcat tgactatggc aacttgattg gtcagttccc gctgactttt
     5701 caagccccga caatttagct gctactaaat tgtcgggtgc cttttttagt ttttattcgt
     5761 ttttcattct atagaaagcg cttcaataaa tcaagacatt tttttgttag aatgctaata
     5821 ataacaaaag gttttaactc ttcttatgta cattacaggt aaaaaaacaa aaaaatttct
     5881 cttttttttg aattctgaac aaatagtact ggagtacaaa agacgaggaa gagccactat
     5941 ctgctcttcc tcgtcttttg attctcacct gttattcatg attctttttc tcagtttccc
     6001 gatatttttc cattttacga ctaaaaagct ctccgctact tggtggcgta tatgtgatgg
     6061 catttgctcc tgcttgaatg gtctcagcaa tactttcttc cgttggacca cctgtcgcaa
     6121 tgataggaag ttcaggatat tttttacgaa aatggcgtac tgcttctgca gtttctttac
     6181 ctgcactgat attgataatc gaaataccag ctgctaactt ttcatctatt tctgtatatt
     6241 tcgacgttac cgtactgata actggaatat caactactcg acaaacttct tttactgtct
     6301 ctaaaggcgt aggaccgttc aaaacgaccc ctaacgaacc ttgcgcttct gcgaacatgc
     6361 tcatgtagga agaacgagca ccttgtgtca aaccaccacc tacaccggag aaaactggaa
     6421 tgtcagctgc ctcaataatg cttttagtaa tcgctggatg aggtgtaaaa ggatacacag
     6481 caattactgc attcgcattt gtattacgta tgatcgcaat atccgtagta aagatgatcg
     6541 aacggatcaa ttttccaaat atcttgatcc cgcttgcttc taaaatgatt tccggtactc
     6601 ttacgatatc ttttctcaat tccgtcatga tttctggcgt aaattgttct gccatcagcc
     6661 acttctcctt tgctatctcc ggtcatactg attcaattga tacgtctcga tgacctggat
     6721 gattttttgc gcataaccgg ggtctgtcgc ataacctgca tcttgcaatg cttgtgcggc
     6781 ttcctgatag ttggtggccg taatcacttt ttcatacagc gcaggattcc aatctactcc
     6841 ttggacgaac agttgtgtat ggtcatccat ggactgttcc catgaatcat acactttgaa
     6901 gtctccttgg atagttatcc attcttcgtt aacaaattcc tttgtttcca ggcttacttt
     6961 cttctgatcc ccataagcct tgatcccgaa aagattattg tatttgctag ccaaggtact
     7021 ttttccccaa tttgattcta atatagcttg tcctaaaatg atactaggaa gcactccata
     7081 accatcttgt agttcttggg catgcggaac aagccgttca ataaattgct gatgggattg
     7141 cgcttcattt tcttgctgat tatcagtaaa aggagaagac agattcttca aggaaaaaac
     7201 aaaggctaag agtatcactg ctacaccggc aaccactgct ggccaacgga tattcttgcg
     7261 cttttgcttt ttccactttt ttttcgccat gatcaattcc tacttttcat ttaattcatt
     7321 cagatattct tctagcgtca attggtgttt ggtgatatac tcagcgtttt ctttaccgac
     7381 ataacgtaag tgccaaggtt catatgtgat cttagttata tcttcgtgtc cttttggata
     7441 acgaacgata aatccatact gcgcagcatt atctgcgatc catttagctc caggctgatc
     7501 tccgtaactt gcatccaata cagtagaagg ataactgttg taccagtttt gatcgacgac
     7561 atcgactgcc agacctgtgt ggtgttcgct gtatcctggc tccgtaatcg tttctttagt
     7621 aatctttgtc gcctcctctt ctgatacacc ttgacgactc atcacatctt ggacattttg
     7681 ttcgaaaact tgttgttggg atgatacaga ccggtaagct gagaccatca ctaatggaaa
     7741 tccagctgct tcagcagcct tggcaaatgc ttcatattct ggagcaatgc gtttgtcgat
     7801 caagtatccg ttacttaatg atgccaattg agtagactca tcaatttctt tttctaattt
     7861 gtgatctgga ccaaccaaaa ctagcttcca atcgtctgtt ttaacatctg gcaattgttt
     7921 ggacgtacga tcttttgttt ctttgtcctt taccttgctg ctggttgttt gactgttctt
     7981 ttctttacta gcagttgttt tggtgcttgt tcgttcgact gcttgattgc cggcttgaac
     8041 atgcgaatca tcatcgctaa aaataacata gcccattgct gcgatcataa tcacaactgc
     8101 tccaaatcct aaaaatttat tcacttgaaa aatcctttcg ttgttcttca cgttccccat
     8161 tcgcctcatg aacgtaagtt tcttccagat tttcattcac ccaatcgaga agttcgattt
     8221 tttctccttc gatctgtttt tgaacatctg ctggcagacg aaaattcaaa atatcatcat
     8281 agccccattc atcatattga atacttactt tcaagtcaag gtatctacca gcctgctgat
     8341 tatttttcaa agggatcagc tcgacttttg ctgctttgct gctaagccat ttttgcgcga
     8401 tgcgtgtttt gatttgcttg tactctgcaa cggcaacgtt tcgtttttcg ggaaaaataa
     8461 tcgtttgacg caaaatcttt tgcgttagca tccattcata atcagtgaaa agatttttta
     8521 ctttctgcat cgcttcagat ggcagatttt tctcgccatc tttccatttt tgccattgtt
     8581 ctggactgat cagcaagtat tcatctaaaa aatttttttc agtttcaaac tgttctaata
     8641 atacactcat aataagatca atatatactt gatgcacgtt tatcccctcc ttttaactat
     8701 tctacttaag ttttgatcaa aaagcacctc ttaaatcgct gctttcattt tttttaatgt
     8761 ttcaagtcct ctcctcttta ttcttcgtga tttttgttat aataatagac agacaagaaa
     8821 ctcaatccaa ctatgaagga gcgttttcag atgaaaaaat atcaatgggg aatcgttggt
     8881 cttggtacca tcgcacatga gtttgccgaa agcttcaatc aagagacgag cgaactaaca
     8941 gcagttgctt ccagaacttc agaaaaagcg gaaaacttcg ctcaccgtta caatattcca
     9001 aaagcttacg gaagctatca agaaatgtta gacgatgctg aaatcgacat cgtgtacatt
     9061 gctgtaccaa atagacagca tatcgaccat atcttggcag cattggaagc aggcaaacac
     9121 gttctatgtg aaaaagccat cacgatgaat aaaaaagaac tggctgacgc tatgcgctta
     9181 gcagaagaaa agaatttgat cttagcagaa gcgatgacaa tcttcaacat gccactttat
     9241 cagcagttac gctcaataat ggatacagga aaactggggg ctttaaaaat gatccaagca
     9301 cctttcggca gttacaaaga accagaccct aaaaaccggt tcttcaatcc agaattagct
     9361 ggaggagctc tgttggatat cggtacgtat gctgtatctt tcgctcgttt ctttttgagc
     9421 tctcaacctg aagtagttgc ctctacaatg gttccttttg aaacaggcgt ggacgaacaa
     9481 tccgtcacta tcttgcgtaa caaagaaaat gaacttgctg ctgtcagctt gacttttcag
     9541 gcaaaaatgc caaaagtcgg tatcgttgcc tttgagaacg gttatgtgac gattgctgat
     9601 tacccgcgtg ctgatcgtgc ggagatctta tttacagatg gtacaaagga attcatcgaa
     9661 agcgggaata catctgaagc aatgaattac gaaataaaga atatggtaca catgattgaa
     9721 ggtgacttgc ccaatcgctc tctcttcttg acaaaagatg tcatcgaaat cttggatcaa
     9781 atgcaagttc tatggaaaaa tatgtaattt taaaaggttg tgaaaaagct gttctgcatc
     9841 aagtaataag aacagccaaa caaaaatagc tcctcctatt tttcgtttgg ctggacttat
     9901 taccgcagag gaaagctttt gaacaccgtt taagaaagag gttgtgagcc taccgttcag
     9961 ctccgagtca caaggagcaa tttcaaaaaa cagcttctca tgtttgttga atattgtgac
    10021 ttgtgaccga ggagttgaca ggagaacacc gtttaaagaa gaggttgtga aatcagcttt
    10081 ttgacctgaa tattagctga attcacaacc tctatttttg gattttttgt tgggagaatt
    10141 ctgccttgat ttcaataaaa cttttaaaag actgcaaaaa ctggaacaat tctgctcgat
    10201 cctcgaattc agggcgacta gtcggcagag gcatttgccg atacaataca taaacttctt
    10261 caatacgtgt taaaatttgt ctgccatcat ttgactctga aaatgtttct gcagtataaa
    10321 tcaataaacc atagatatgt tttccataat aagctggttg ctggattctc tctagatttt
    10381 ccagcatgtc tttaatcaca ttggcctgtg ctcttctcat agagaagtaa gtttcgtagt
    10441 atagcggctg tctgagccag tagttttctt gatgttctct ggcatcttct tggctttcgc
    10501 gaatgaatgt cagtaaatgt tcgcatttga tctgcacatc ttctttattt tctgataaaa
    10561 gaaattctgc catttgtctc aaaatatttc gaaattcttt ttcgatctgt atttggtttt
    10621 ccttcagctg cttcgtacga tctggcatgt aaacatttgc aagcaaagca aatactaatc
    10681 caatcgccat taatcccgct tcattgccga tcagctgcca agacatgttt tcctccacta
    10741 aataatgagt gaccaaaacc gaatttacta ctatcccttc ggttaatttg aagcgagagg
    10801 caagcgggat gaacaggaat aaaaaaagac tgaagccaaa aatagtatac ccaacagcaa
    10861 aaaaactgaa cagtgctgtt gccgtagcta atataaaggc tgcgattcga ttcatgcccg
    10921 tacgcaatgt agagcgcttg gtatttccta cacttaataa tgcaatgatc cctgcagctg
    10981 gccaatacaa cagcgataag ctacccgctg tgaccatcga aaagaagaca ctgatcactg
    11041 ttttgactgt tcgcattcct atacgcattt acttccccct tttttatttt aattatactt
    11101 gaataatgaa aaactagcta tcagacaaaa gtttaggtat gatagtaaaa agaccaaaaa
    11161 ggagaatctc tatgcaattt ggccgttttc gactaggtat gcgtacgatc aaaagtgcct
    11221 tagctgtttt tttatgtatc ttgttttttc atttgactca acgaggaatc ccgatgattg
    11281 ctgcattatc tgctgttttt tctttgcgtc aggacttgac tacaaccgtt tcttttggaa
    11341 agtctaggat catcggcaat acattaggtg gtacattagc tatcatttat ttttttgtca
    11401 aaagctattt tcacaatgat tttttggtag aattgatctt gcttcctatt ctagttatta
    11461 ttgtgatcgt cgtgtcagat ggtattaaca ataattccgg tatcatttcc gcaatagcta
    11521 cgttgttgct gattgcgttg agcatccctc aaggcgaatc cgctttgtat gctatccaac
    11581 gtgtactaga tacttttata gggacattca ttgcaatcgg tatcaatttc tttcttcgtc
    11641 ctcctgaaac agaaaagaag gaagaactat cagaagattt ggtcgaactc caaaagaaag
    11701 aacgattgct aagaacaaat ttacaagaga tagaagctaa gatccaaaaa caaaagaaaa
    11761 atagcgagga ctaattttct tttgcaataa aataaaaaaa tggagagaag aactttaaaa
    11821 agtcttcctc catttttgat tgaacggtga agtgtactac tttattcttc cttcatcgag
    11881 ttttgttctg cctcttttgg cgcttcatac atatttactt ggctagtagt cccacgtgtc
    11941 gacaaattct ctacgatttc cttcaagtca agtcctgttg tcgcttttag tgtttcttga
    12001 gtagaagaaa gcaaatttgt ggcataattt gtcactcggt tcgctccact ggtttctcct
    12061 gaactgcttc ctgtatcgac aacagagatt ttttcgatat ttcctagcgg ctgtgctgct
    12121 tcgcgcatca attgcggtag catatcgata accatactca agacagctgc ttcgccatat
    12181 tccttgaagg cattggcgat cttttgtttt gcttcggctt ctgcttttcc ttttgctagt
    12241 gctgcttcgg cttctgcttg tccagctaaa cgtgtcttat tggcttccgc ttctgccagt
    12301 gcttccacac ggaatcgttc tgcttcggct tcagtcactt ctttgacttt ttgagcttgt
    12361 gcttcttgtt ctttagcgta ccgatcggca tctgcttttt tcttcacttc cgaatcatat
    12421 tgcttttcac gacgtgtgat ttctttttct tccagttcga tttgtttttg acgttctacg
    12481 attttaatct gcatttcctg ttcgattact tgttgttgtg ctcgggcact ttctaaattg
    12541 taggcttggt ctgcttttgc tttagcaaca tcttgttctt gtttgtaggt tgctaatttc
    12601 agttcttttt cttttaatga ttctgcgatc tctgtttgtc gctgtaattc agctgcttgt
    12661 gattcttttt ctgcttgagc ttttttgata cgcgtttctt ttagagcttc cgcttctgct
    12721 atatctgcat ctcttttgac ttgggcgatt cttggtttcc ctaaagagtc aagataaccg
    12781 tttttgtctc tgacctcttt gatagtgaaa gaaacgatga tcaaccccat ttttgcaagg
    12841 tctacagaag cgacttcttg tacgctttga ctgaacttat cgcgattttg atagatttct
    12901 tctacagtca tagaaccaag aatcgagcgc aagtgtcctt ctaatacttc tcttgcttca
    12961 ttttccagtt cttctcttgt cttgcctaaa aactgttctg ctgctgtagc aatctcttct
    13021 acagatgaac cgattttgat gatcgaagta ccatcacaca tcaccggtac gccttgttcc
    13081 gtatacactt caggggtgga gacgtccaat ttactcgata atagactgat ccgattcgaa
    13141 cgttgaaaaa ctggcaagac gaatgcgcca cctccacgca caattttgat tttgttattg
    13201 ctttcatcaa tgtgcacatt tttgcttcct aaataacttc cactgatgat caacgcttca
    13261 tccggttttg ctgtctggta cttcgctaca aagacgatca gcaacatcag taaaatcaaa
    13321 gctgcgattg cgatcgcgat aaaaacaact ggtatttcca tttaacacac gctcctttaa
    13381 gcttttatca tgctttcata aggcatgaca taacagattc tttccttgat ctcaatgatc
    13441 aatactttcg ttccccgctc agctgtctgt tcttgggggc tgtaaaaaca agctggacga
    13501 ctaatggaac cggtaacact tttcaactga atttctccca tacctcgtac agggatcggt
    13561 gtgatcacaa cagctacttg tccttccaga gttttttcag aagacgacat cgttgattcg
    13621 gcatgtttca tcggcatcaa tatatagaaa ttaagcaaaa ataccaacac tatcgatatg
    13681 cctccactag caataaataa aaataaagat gattgatctg ttaaacgctc acctagaaaa
    13741 ccaaataagc ttgtgaaagt taaccaaggg ataataagca tcggatcaag cgggccatca
    13801 aaatcgaata catctccaaa aataatcaat aggactgcca atcctgcaca aatcagcaat
    13861 gtataccaat aaatcgtctc aagtgcgatt ccccctatca tatacacgcc tccttccatt
    13921 tctacgtcta tcataccaat gtttgaaaat tctgtatagc ttcttttacc gactcagtgg
    13981 tgttttttat aggaaggttg tgtttatcgt tgacaatttg ttaaattttt gttttcatgt
    14041 taaaattttc acaataaatt ctttcgaggt agcctatgtt tccttttttt aagaaaaaac
    14101 cattaaagct ccagaacacg atcatccttg tcgtttgttt ctccacattc gtttctttga
    14161 ttgtttctgc catattgatc aggaatttta ttctttcgat gaaccgatct agtgtgtcag
    14221 ataaaatctc tagcattgct aaaatcgttg ccaatgataa actcatccaa aaaaagctgc
    14281 agactggtga cccgacacat gcgattcaag gaataacaaa tgaaatcatg attgcatcga
    14341 atttagattt tatcgttgtc atggaccctg aaggcattcg atattctcac gcagacccaa
    14401 gttacatcgg tcttcctttc tcaagtcatg aagatgtgaa aaaagtgatt ggaggacaag
    14461 aatttttctc tgaaaaagaa ggaatgttag ggttcggctt ccgctatttc gtccctattt
    14521 attatgatca agagattatt ggtgcagttt gtgttggctt aacaaacaac acgattgaca
    14581 atcaagtcaa agatggacaa aaaatgattg ttttttcatt gattattgga cttagcgtag
    14641 gcatcatagg cgccgttttt cttgcccaaa aaatgaaaaa agtactgctt ggacttgaac
    14701 cacaagaaat agcagaacag ttaagtgaaa aaacgattat cgaaaatgaa gtagatgaag
    14761 gaatcttagc catctcttct gataaaacta tcctcttgat caatacacct gcaaaaaaca
    14821 ttttctacca aacagagcag ctattggatt tcgaagtagg aaaaaaatca gtgataatct
    14881 atatgacgtt ttgtttaaag agacgattga gcatcaaacg accgttcgga ctcaatccat
    14941 gtatttaaat gggatagaaa ctatttctag cgttacacct ctttttagca atgaccgttt
    15001 tttaggagca gtcgcaactt tacgaaatcg atcagaagta atgaaattaa tggaggagct
    15061 tggggggacg aaacaatata taaactccat gagagctcaa acccatgaat tcatgaataa
    15121 acttcatgtc atttctggtt tgattgattt gaagaagtat gaagaagtca cccattttat
    15181 ccagcagcta aatcaaaatt ataaaaaaga gattggccaa gtctcaagtc tgatcaaagt
    15241 acctgcaatt gccggttttt tattaggcaa aataaacgaa gccaaagaac aatctgttga
    15301 aatgctgtta gaaagcgatt cttttatccc taaaatggag atggatgact ccatccattc
    15361 acttttgcaa gtgttaggga acttacttga taatgcaaaa gaagccgtgc tacaaaaaca
    15421 tgaaagaaac ggacaggtcc ggattcattt aaactatgac acagaagctg aagtattcat
    15481 cgtgaccgtc tgcgatgacg gtgtcgggat atcagaagaa atctcagaaa aaatatttac
    15541 gctaggtttt tcgacgaagg gaatcaatcg tgggtttgga ttgaatctca tccattcaat
    15601 cgtcaaacgc cacggtggat ttttagaatt ttccaaacaa acaaatggcg gtacaatcgt
    15661 ctatctagaa ttaccaaaaa aggaagatca aaatgatgac aaatgtcctg ataatcgaag
    15721 atgatccaat ggttgcagaa ttgaatcgta cttttgtgga aagcatccct aatatgaacg
    15781 taatcgaaag tttcagaaat gtagaagaag gaaaaaaata tttcagacaa aaaaacgcag
    15841 atctgctgct gcttgatgta tacatgcctg gcgaaagtgg tatccatttt ttatcttggc
    15901 ttagacagca aggaaatgat atcccagtga ttttgatcac tgctgcagat gatatcgaaa
    15961 ccataaagaa tgtgatccgc tacggtgcct tagattattt gatcaaaccg ttcactttgc
    16021 aacgttttca gctggcgatc gaaaagtatc ttgctttctc tcagacaatc caaacgactg
    16081 aaaaagccga tcagaaattg atcgatcaag tattcaacca caccactccc attcacgaaa
    16141 taccatcatt catcgatttg cctaaaggtc tgtcaaaact gactttaaaa aaaataagtg
    16201 catttattct aaaagaagat actatttttt caacaaaaga actggctcaa aaaagctcac
    16261 tgtcacgtat atccgtcaag aaatatttga attttttagt agaacaagat atattgaacg
    16321 aaagaatcaa atatttggaa atcggccggc cgctaacctg ttatgaattg acaaagaatg
    16381 ggcggaaaaa gctcacagat tactgttgag tcatcgaatt atcccataca aaatagtcca
    16441 agtatacaga agtaacaact gtttacttgg actattttta aacattttat tgacgaatat
    16501 ccatgttttc ttatcttaat tgacgggctt taggaagaga tacctcattt ttcgtttttc
    16561 cagtttctat tatttcacga aagttctcga aacttgtcga aatcatattt gataatgcct
    16621 catctgtatt tgaacctaca tgcggggtta caagaactct agggtacatt tctactaatt
    16681 gctggatagc agggtctgga atcgtctccc atggcttgaa ggatcggaaa aaaacctctg
    16741 tttcatttgc aaaaacatcg gttgcaaaac cagctaaatg attgcttttc aaagctctta
    16801 aaatagcttc attatcttga agctctccac gggctgtatt aatcaagatg ctaccttttt
    16861 tcatttgagc taaaaatcct tcattgatca tttgatcgtt ttcacctggg atatgaggta
    16921 catgaatact gacaatatcg cttttggcta ataactcttc aagcgagtgg aacgtcaata
    16981 cttttttagc agcttctgtc tgatgaatat cgtagccgat cactttggct cctagtccct
    17041 taaacaaaac agcttccgtc aaccctatcc tgcctgttcc tatgatccct actgtgcaat
    17101 ttctgatttc acgactaaac atttgctcat ccactcgaaa atctttgaaa gatgttcgga
    17161 tcgtcgtata cgcagtattt ctcaaaagca tcatggcaaa agtcaaagat aactctgcta
    17221 tcgcatttgg cgaatacgat ggtacacggg ctactgtcat accaaaatca gctgccgctt
    17281 gtagatcgat atgattgact cccacagtcc gtgtaaaaac ataacgaatc ccatattcct
    17341 gcatttttgc aagattggtc tcatctgcta tacaatttcc cctcaacaat acagcatcat
    17401 ggtcctttgc tgacacgata ttgtcatgtg tcaacagttc ttcataaaga ctgagatcat
    17461 aatggtaaat attcaatttt tcaaaaaaat ctatctcgtt cggacgaaca ccgtaacatg
    17521 cgattctcat actttcttcc tccatttctt atgaaaataa acctatgcca tgaatcattt
    17581 gtaatacaac gatccataca ggtgtcagta ccacagcaaa aatagttgac agtaaggatg
    17641 cattggaggc taaaagtgcc tccctatcaa aactgatagc ataggctgct gccactgttg
    17701 cagctggtgt agccatcatg ataacgattg ttgccagtgc ttcaaaattg actggtagaa
    17761 tattcgttgc cgtcaaaact gctagaatca cgatattcaa tgcaggtaca aataccactt
    17821 tgactagact gtaataccat gaagtttttt cagatgcagc ttttttgaag cttacttcac
    17881 ctaatgttgc gccgattgct agccacgcca atggtgaact taaaccagct aaataatcca
    17941 tcggctgaaa tagccacggt gcagtctggt caattcgcaa aaacgctact tggcttatct
    18001 ctccttctgc acctgtaact gacacttgtg gcagataccc ttggaacagc caaatgaaaa
    18061 ggccggcaaa agtagcgata atgattggat tgacgaacat cgctttgata tttttagctt
    18121 ccatcttcaa gccactcatt tttatgtatc cataagaata caaaaagatt cggtagccaa
    18181 tattgaaaat tgaggaatac atcacgccga caggaccata aatcgcactg acgataggcg
    18241 ttccgaaaaa ggtcgtagaa ccaaagatcg tcaggacacg caacgttgtt tgcttatccc
    18301 ctttatagct taagaaaaaa ggttttgaaa ctagaataag aataatataa ataactaatc
    18361 cccaaatcaa gacattgatt ccttgcatca gtaattctgt attgatatct tgcatgaaag
    18421 ctcgaaatgc cagtgctggc aaagccactg ttaagacgac tttcgataga atttttccaa
    18481 cagattcaga aaaaatcccc ttttttctac aaacaaaccc taataatata ataaaaactg
    18541 ttgaagtgat tgcactgaca atcgacatat ctgtcaaagt cgatcgaaaa atgtcaaata
    18601 agttcatctt tctcctccta attatgttca tttattca
//
LOCUS       DAJSMI010000041        18568 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJSMI010000041 DAJSMI010000000
VERSION     DAJSMI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.041"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..210)
                     /locus_tag="ON430_001141"
     CDS             complement(<1..210)
                     /locus_tag="ON430_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3219068.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPL"
     gene            476..820
                     /locus_tag="ON430_001142"
     CDS             476..820
                     /locus_tag="ON430_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR3219069.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            821..1240
                     /locus_tag="ON430_001143"
     CDS             821..1240
                     /locus_tag="ON430_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR3219070.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1332..1682)
                     /locus_tag="ON430_001144"
     CDS             complement(1332..1682)
                     /locus_tag="ON430_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219071.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1648..2979)
                     /locus_tag="ON430_001145"
     CDS             complement(1648..2979)
                     /locus_tag="ON430_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCR3219072.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3235..3801
                     /locus_tag="ON430_001146"
     CDS             3235..3801
                     /locus_tag="ON430_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3219073.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3946..4033
                     /locus_tag="ON430_001147"
     tRNA            3946..4033
                     /locus_tag="ON430_001147"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3978..3980,aa:Ser,seq:cga)
     gene            4367..5341
                     /gene="bsh"
                     /locus_tag="ON430_001148"
     CDS             4367..5341
                     /gene="bsh"
                     /locus_tag="ON430_001148"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR3219074.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5666..7561
                     /gene="asnB"
                     /locus_tag="ON430_001149"
     CDS             5666..7561
                     /gene="asnB"
                     /locus_tag="ON430_001149"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCR3219075.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7756..8202)
                     /locus_tag="ON430_001150"
     CDS             complement(7756..8202)
                     /locus_tag="ON430_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR3219076.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8430..8579
                     /gene="rpmG"
                     /locus_tag="ON430_001151"
     CDS             8430..8579
                     /gene="rpmG"
                     /locus_tag="ON430_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3219077.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8667..9185
                     /locus_tag="ON430_001152"
     CDS             8667..9185
                     /locus_tag="ON430_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3219078.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9262..10218)
                     /locus_tag="ON430_001153"
     CDS             complement(9262..10218)
                     /locus_tag="ON430_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3219079.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10384..11190
                     /locus_tag="ON430_001154"
     CDS             10384..11190
                     /locus_tag="ON430_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3219080.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11187..12176
                     /locus_tag="ON430_001155"
     CDS             11187..12176
                     /locus_tag="ON430_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR3219081.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12173..13177
                     /locus_tag="ON430_001156"
     CDS             12173..13177
                     /locus_tag="ON430_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR3219082.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13309..13851
                     /locus_tag="ON430_001157"
     CDS             13309..13851
                     /locus_tag="ON430_001157"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR3219083.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13871..14569
                     /locus_tag="ON430_001158"
     CDS             13871..14569
                     /locus_tag="ON430_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR3219084.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14590..14802
                     /locus_tag="ON430_001159"
     CDS             14590..14802
                     /locus_tag="ON430_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCR3219085.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14802..15764
                     /locus_tag="ON430_001160"
     CDS             14802..15764
                     /locus_tag="ON430_001160"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCR3219086.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15782..16177
                     /locus_tag="ON430_001161"
     CDS             15782..16177
                     /locus_tag="ON430_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3219087.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16340..16705
                     /locus_tag="ON430_001162"
     CDS             16340..16705
                     /locus_tag="ON430_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCR3219088.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16702..18513
                     /locus_tag="ON430_001163"
     CDS             16702..18513
                     /locus_tag="ON430_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR3219089.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
BASE COUNT         5961 a         3132 c         3838 g         5637 t
ORIGIN      
        1 aagcggacgt ctaccacagt ttgatttgtt tttcttatac tgttgataat attctaaggc
       61 tgattttcct tgcttgaaga acagatatac tttatggatc gtttgtcttg aacgattcaa
      121 gcaatgggca gttttagcaa cagattgatt cattttgaaa taagactcta ttattacaag
      181 ttcatccgtt gtaagatggg tataggtcat ttgtactcac tccttataat tctttggtcg
      241 gaactatttt gagtgtagca caaatgactt ttttagttgt ctagcttaat tttacaatcg
      301 gcgaataaaa aaagcgttag caactatgct taaaaagcat atcgactgaa taaactaagc
      361 attggacagg ctgaaagact acatttaaaa tgaaatgcga agaaacaata gaaaataaag
      421 ttgccttaga accatagttg aatcacctaa cagtcgtgaa gggagaataa gagaaatggc
      481 agaaaaacaa accgcaggac gagatcaatt aggagatttt gcaccaaaat ttgcagaatt
      541 gaatgatgat gttttatttg gacaagtatg gtccagagaa gaattatctg ctcatcagcg
      601 tagtatcatc accatatctg cattaatttc aggaggaaat tttgaacaat tgccagctca
      661 tctgaagatt ggcaagcaaa atggcattac tagtgaagaa atagcagaaa tcattaccca
      721 tttagctttt tatgtaggat ggccaaaagc ttggtcagct tttagtttag caaaagaaat
      781 ctatttagaa gaaaatagcg aagataagaa agaggcgtaa atgacgacaa aacacgaaga
      841 aatacacaat ggcgtgattt ttccagcagg tgaaaaaaac gaagcttacg ctcaagtatt
      901 taccgggcaa agctatctga atatgttagt gaatgatccc gaagtttctg ttggtgtagg
      961 taatgtaact tttgaaccag gttgtcgcaa tcactggcat gtccataaag acggctatca
     1021 aatcttactt gtgaccggag gagaaggctg gtatcaggaa gaaggaaagc cagcaagacg
     1081 attaatggct ggagatgtcg tcgtaacgca caaagggatt aaacattggc atggtgcgac
     1141 aaaagacagt tggtttgaac atttggcgat cactgctggt aattcagagt ttttagaacc
     1201 tgtaacagat gaagtgtatg aaggattgga aaatcaataa tgtcaagctt gaacagctcc
     1261 tgaaagtaga ttttaatcga actttcagga gctgcttttt tgattcagtc ttttataaat
     1321 agaaaatagg gttaataata ggaatgagaa gcacgtcgaa agatcttaat attgataatc
     1381 ggctgggcat acatgatttc cacgtcatca atcaaactac ctaacagatg ataatcttcc
     1441 cgatcatgtc ggggattacc taatagttct tgataataga tttccatact agggtcccga
     1501 ggattgttca gcaaatcagc aagtacatct aaatcttctg gctgagtttc tgccgtaccc
     1561 tgtatctcta tgaaaagccc ttcctcagca aattctactc caatacgtat ttcagttatt
     1621 tcatgggaat ataaataagt aagaagctca tcgatgattc tttttgataa ttctttttcc
     1681 aagtgtcttt ttttcctttc tgacagcggt aaagttatct agtatcggta caaggatacc
     1741 tgcaacgaaa ccagttgaaa aaccactgtt atatagattt agtcctccgt gcaaagtgct
     1801 gacgttatgg acaagagcaa tatgtagtac accagctatt acaccgtaaa aactgccgta
     1861 aaaaccgctt atgggcgcga gacctgtccc aaacacagct gcaacaagca tgccagtact
     1921 ggtgatctca tgaaaagttc caaacaaaga agcaataaat attccgacta gtatcggaaa
     1981 actgtttttt aaatgacaac caaaagcact gaaacccacc gcagataaaa tagcaccgat
     2041 gacaggtcca tttaactgac cgccaaccag caaaacataa gaagacagca tcagaccaac
     2101 taagcctatg ttgatcaaag tagcaccaat accagaatta gcaatgaaat cagttgtcaa
     2161 ttttccagat gaatcgaaaa tttctcgaat ccccaaaagc cgaaaggaat tataatagaa
     2221 accaattccg actgtcagcg aaaaaaagcc aaaaataatc caaatgagag gaaagtgata
     2281 ttcattgaaa accaatgtat tttcgacgat tgaatagctg aataacctca aaacagcagt
     2341 aaaagtcatc gcaatcaacc cagaggtgaa tccaatatta taaagtgtga atccacgatg
     2401 gaaaactaaa gtttgagcag ctaagggcgg aagaagtaga ccaattgcta tgccagctaa
     2461 attccccaat aaaataccta tcagtaaagg aaaacgcata ccaaaagtga tataactaac
     2521 gaccggtgat aatgcgctac caaacagtcc gatcatgatg tactgagaaa aaggtttatt
     2581 gacaaataca gcgtatagat acacacctaa aatgattgag atagaattat aaaggttttt
     2641 gccaaaaaaa gagaaaccgg taactgttag aatcgaagca aataagggac cgtttaaaat
     2701 caccttgtgc cgataagcta aaagcacaga aagtaaggtc aaaatcccta catttatgaa
     2761 tgcgcttccg aaaccgccta aagcaaaata atctgtcaaa agattgcttg gcgaggtaag
     2821 aatagttagc aatccactcc aattatcagt aggagtatta gaaagcaagc caataaaaag
     2881 aaaaaaaaca cctgtaaaac caagataaaa atactttctt ttctgtgaaa cgattcgttt
     2941 ttgaggatga atataaataa cgtcagtttc gttttccatt acacaccttc tttttcattt
     3001 tctttaacta taacttgaaa tgaaagattt ggacaactat catttaggtc aaaactaaaa
     3061 atgtaaggga acccattgaa tttaaccaaa tttttttaat tttgcaaaaa aaagttaaag
     3121 aagactgaaa aaaaatttaa aatataacgt tttgagtaaa atgtttgcaa aaaatgctaa
     3181 aaatagggta caataaaaca acatatactg cagtaaagaa gtgggggagt tttaatgaga
     3241 agaaaagtct ataccaagga tcaaattcta aaagctgctt atgatgttgt tgcaaaggaa
     3301 gggtttaaag gatttactgc gagaaatatt gctaaaaaga tggggatttc gacacagccg
     3361 atttatctgg aattcaaaaa catggaagat ttaaaaaata cgttgctgga ttctatcttt
     3421 gaacaactaa aagaagatgt tttccctatt gaacgtactg gtgataaaat cattgatctc
     3481 ggtttgaact atatctactt tgcaaaaaag catcatagtc tttacatggc attgtacatt
     3541 gatgaacatg gggacggaaa gaaaatgcat gatttttcat acaattattt ccgtgaaata
     3601 gttaaagaag atgaaagata caatcaatta tcggatgaat atgtcgatgc tctgcatact
     3661 ggtacatggg tagcagtgac tggcttagcg tcattgatgt catcaaacat catgcatcca
     3721 acagaagaac aaatcattgc actgatcaaa cggacaattg actcgatttt agcattagat
     3781 aatcctgaaa taatcagata gtgttctatc agtatgaatt tgatcggtaa gtccgttggc
     3841 tgcaaactgt caatggactt ttttgctaaa aaaagcgatt tctgttattt atctattaga
     3901 aataggcttg acatttcgtg aaaaaagttt aaaaatagta cttacggaag gttggcagag
     3961 tggtaatgca ccggactcga aatccggcga accggctaat accggcgcgc aggttcaaat
     4021 cctgtacctt ccttatatca acgattagag gttgtgaaga agctgtcctg catcaagtaa
     4081 taagaacggc caaacaaaaa tagcttttcc tattttttcg tttgattggg cttattaccg
     4141 cagatgcaag cttttgaaca ccgtttgttc ggaataaggg gctgtgacaa gacttttgtc
     4201 acagcccctt attgttgtac ttttaaccta caatattttt tgattccccc tgagtactat
     4261 tttttataaa tgctagtaaa tcaatcttct ttggcctttt tttgataaaa aaaatactag
     4321 atgtaagata aaagtgtaaa aacgatttgt tttaggacgg gaaacgatgt gtaccgcaat
     4381 cacttatgca acgaaagacc attattttgg aaggaatttt gattatgaga tgtcttataa
     4441 tgaagtggtc actattactc caagaaatta tcgtttcgat tttagaaagg taaaaaattt
     4501 agataaacat tatgcaatga taggaatagc agctggggta tccaactatc cactctatta
     4561 tgaggctaca aatgaaaaag ggttgagtat ggcaggactg aattttcctg gcaatgctga
     4621 ttataaagaa ttacaagaag gaaaagataa cgttgcacca tttgagttta ttccatggat
     4681 actagggcaa tgctcaacca tagacgaagc taaagaatta ttggctacta tcaatctagt
     4741 caatatcgat tttagtgaaa aactaccctt gtcaccttta cactggttat tagctgataa
     4801 agaaaaatcg atagtaattg aaagtatgaa agatggactt catttatatg ataatcctgt
     4861 cggtgtgttg acaaataatc cgccatttga ttatcaatta tttaatctga acaattatcg
     4921 ctcgctatca aatggaacac cagaaaatca tttttcaaac cagatcagct tgaatgttta
     4981 tagtcgtggg atgggaggat tgggcttacc tggagatctc tcttctgttt ctcgatttgt
     5041 aaaagccaca tttactaaaa tgaatgctgc atcaggtgat tcagaatctg aaagtatcag
     5101 tcaatttttt catatactag gctcagtcga gcaacaaaaa ggagtatgcg atactggaga
     5161 aagaaaatac gaatatacga tttattcttc ttgctgtaac gttgataaag ggatttatta
     5221 ttatcgtacg tatgaagaca gtcaaattac tgctgtaaat atgaataaag aagacttgga
     5281 tcgtcatgaa ttggtcagtt atccagtcat aaaagagcaa cagataaact atgtcaatta
     5341 attaatgaat agatccgttt ggtatcatat tttctgatac agtcataatt agacacgatc
     5401 tttaggtcgt gtcttttttt gctggttttt ttggaaatag catccactaa tattttttta
     5461 acacgaagag aataaggtag cctcaaaaaa gaaaacatgg taaattagat attgggactt
     5521 tattttctgt aaaaacagaa actttcatct tttgttttca cgtgacgaaa ttgtaagaga
     5581 gaaaatattc gtccgctatt tattctatga taagatgcat atgaatgaaa taaacagtaa
     5641 ttttatatta ggatgtgaaa gttaaatgtg cggtattgta ggttttgtta acgacaagga
     5701 caacaaaaaa acaattatta atacaatgat ggatcgaatc gttcaccgtg gacctaatag
     5761 ttcaggagaa tatatcgata aacatgttgc tttaggattc agaagattaa gtattattga
     5821 cttagagggc ggtactcagc ctatttacaa tgaagatcgg acaaagatca ttatttttaa
     5881 tggcgaaatc tataattacc agcctttgag agaagaattg attgcagctg gccacgtttt
     5941 tcaaacacat gcagatacag aagtcctttt gcatggttac gaagaatggg gaacagaatt
     6001 attgcaaaag atccgcggaa tgtttgcttt tgctatttgg gataatgaaa agaatgagtt
     6061 attcggtgca agagaccact ttgggatcaa accatattat tatgcagaaa tgaacggaac
     6121 atttatgttt ggttcagaaa tcaaaagttt tctgcctcat cctgatttta acaaagaatt
     6181 gaacaaagaa gcgttgaaac cttatatgac tttccaatat tctccattaa acggcgaaac
     6241 attcttcaaa gatgtctatc gtttgcctga aggccattat tatacgtata aagatggaaa
     6301 aatagacatt cagcaatatt gggatgcaga ttttgaaaca aaagaaacgc attcccgaca
     6361 agaatggatc gaaaaaatcg acgaaaccgt tcaagcttct attgaagcac atacggtcag
     6421 tgatgtagaa gttggttctt tcttatccag cggggttgat tccagttatg taacctctgt
     6481 attgaaacca gatcattctt tctcaatcgg gtttgatgat aaaacctata atgaagcgat
     6541 tgaagccaga aaactaaccg aactattgga cttagataat acagcagcag tgatcgacgg
     6601 agacatgtcg ttcaaagcat tccctttgat ccagtatcac ttagatgaac ctgattcaaa
     6661 tccttcttgc gtacctttgt actttttagc aaatcttgct tcacaaagtg tgcgggtagt
     6721 ccaatctgga gaaggggcgg atgaactgtt tgcgggatac caaacgtatg gattccatac
     6781 gaattcaaaa ttcatccgtg tgattgctca aggattgaaa aaattaccaa aaggaacacg
     6841 ctataattta ggacgtaaaa tcggaaaaat gaaaaatttc catggacgga tccatttata
     6901 tgaatcactt gctcctgcaa aagaatattt tatcggtcat gctcgagtgt tcgaagaatc
     6961 agaagcatca gaagtgttaa caccaaaata tcaaacagca ccatctgtgg atgaaatcat
     7021 gactgttcat tacgagaaaa cagaaggaat caaagatgaa gtcaataaga tgcaatatgt
     7081 cgatcttcat caatggatgc caaaagatat cttgttaaag gcagataaac tttctatggc
     7141 aagttctttg gaagtccgag tacctttatt ggatatcgaa gtcatgaaat tggctcaaca
     7201 aataccaagt aagtttttat tgaatcaaaa caacacaaaa gatattttca gacaagctgc
     7261 gaacaaacat cttccggaag aatggtcgaa tcgtgtgaaa ttaggattcc ctgtgccaat
     7321 caaagcttgg ttgaaagaag aacacggcta cgaacaagtc aaagcattat tcgaagcaga
     7381 ttttgcaaaa gaattttttg atcaagaaaa aatcatgaag ttgctagatg accatcacga
     7441 aggacgtaaa gaagagcaaa gaaaaatctg gacaatcttc agtttcttga catggtataa
     7501 agtctacttt gtagatgaaa cgattccgca agcagaagca atcgactatg tgactgtttg
     7561 atgaaaatca aatatcaata aaaaagaggt tttctcctac cgtttaacac tgagtgataa
     7621 gaaacaattt caaaaaacag ctcttcccgt tttatagata ttatgactta tggttcggag
     7681 ttggcagagg aacaccattg tttcgaacat aagggggttg tgacacgatt ttcgtcacaa
     7741 cccctttaca tatgttcaat aatcgatttc tttttctttt gtcccatctt ttttataaat
     7801 gaacaaagtt gattctttgt tttgtgctac ttgctttcct ttttcgattg cttctttttt
     7861 tgtatcaaaa tggttgcttg ccttttttgc ccctttagat ttcacgatcc attgttcttc
     7921 ctcatattct acgatcacat ctgaatcaag cagttttcca gcacgaggat tcgtatcgtg
     7981 ttcatccgtc ttgcttggat ttttttcttt ttcgaaagtt cgtttttctg attcactagc
     8041 atcttcatac catttttttg cttgatcaat ggcaattggg atcgcacggt tatcagggta
     8101 cccttcatct aataatgcat ttgcgatgtc gattgctttt tttcttgtca atttatctaa
     8161 gtttttcatt gatgcaggaa agtccttcat gttccatggc atattagtat ccccctttat
     8221 taaaaaacat aatctatata aacaagtgta agggtattta tacaaaaaga aaaagatttt
     8281 gtttgagaag taaaaccgtt gtatttatcg tcaagtagtt ttacttaaca aaatcgtgaa
     8341 aaacactagc aatctagaaa aaatcatggt aagataatca agtctgagag tcatcttaga
     8401 ggattcttgt aaaaatggga gggaaaaaca tgcgcgtaaa catcacttta gagtgtactt
     8461 cttgtaaaga acgtaactat ctaacaagca aaaacaaacg taacaatcct gatcgtttgg
     8521 aaaaacaaaa atattgccca cgcgaacgca aagttacttt gcaccgtgaa acaaaataat
     8581 gtttttaaag ttctcaattc cttgctggtc aaagaattga gacttttttt atctcaaaac
     8641 atctaaaatc ataaggagcg gataatatgt ttatttccat gtgggcacaa gataaaaatg
     8701 gtctgatcgg caaaaatggt ttgttaccat ggcgattgcc aaatgatatg cgtttctttc
     8761 gcgaacacac aatggatcga attttagtga tgggaagaaa gacctatgaa ggaatggggg
     8821 atctttcact tccttatcgc catatcatcg tgttgacaac gcaaccaaat tttcgcacga
     8881 aagaaaatgc ggaagtaatg cattcgatcg atgaactttt gtcgtatagt caaacgatct
     8941 cagaagatat ctatgtttcg ggtggtagtc ggatttttca agaacttctt ccttacactg
     9001 gtcggatctg gcgaacattg atcgatagca cgtttgaagg cgatacgtat attgggaata
     9061 ttgatttttc agacttcgcc cttatagagg agtatgaggg gataacggat caagaaaatc
     9121 tatatgccca tcgttttcaa aaatgggaaa gagtaaaaaa tagggttgct tcaaaagaag
     9181 aataaaatct atgcgtctag tttgttaata agaaaaaaag catctcacga ctaatcgtga
     9241 agatgctttt tttagttaaa atcaatttag tttttctttg atatcattga ccatttcctg
     9301 actggcaata ggtccgttat atagccaact agattcaggt ccgaattgca gtacatgatt
     9361 gtctgtcact gcttttagat tcttccagct tggttcattg aacatggcag catctttgtc
     9421 actgttcaca aggatgatgt agtcagcatc taattcagct agcttttcag aggaaacagc
     9481 agaccagtca gaagtggctt ttttggagac ctcttccact agatcaggaa caccaaattc
     9541 cagatcgtcg tatacgattc ttccacttga ccgatttttg ctgaccataa aggctgagtt
     9601 gttggtcacc cacaacactg cagctgtctt gttctcgatt ttatctgcta gttcttctcg
     9661 agttttcttg atggattttt gataatcgga aatgattttt tctgcctctt tttcttttgc
     9721 caaagctttg ccgacatctt ttagttgttc ttcccatgtg acatctgttc cgttttttac
     9781 aacataagtt ggcgcgattt tgctatattg ttcgtatttc ccgccttcga cagtagcgga
     9841 agaagaaatc aacagcaaat caggctcaaa gcttaacact ttctcataag gaaggtcata
     9901 agaaatcgtt gggacatcct ttaaatcatc ttgcagataa tcttgaatgg aaccactgcc
     9961 aacagtccac tgggcgatag gtttttcccc tagtgcaatc agatagtctt ctaagtaact
    10021 gccgatgatc cgtttgggcg cgtttggaat ctctacttta tgtccaagtg tatctgtgac
    10081 tgtatgagta ctattggatg aagtcgtcga attggcagtt gtagctgttt ctttatctga
    10141 agagggggag caagcgctca gtactccagc cagcagcaat atgctagata atgtgaatac
    10201 ttttgttttt aatttcattt ctttcctcct aacataattg agaatctttc tcattggtta
    10261 ttattcctga tatttcgaac gctgtcaacc gcttcatttg gctatagata tgaaagtagc
    10321 gcttgacgaa atgttgaaac agcgtcataa ttgagaatga ttttcagcga aaggatgggt
    10381 gaaatgacga gattaagagc agctgacatt gcagccggct acgaaaacaa aaaaatcatt
    10441 gaaaacatgt ctgttacgat tcctgaaggg aaaatcacta gtttgattgg gccaaatgga
    10501 agtggaaaat ctactttatt gaaaagtttt acccgtatac tacctgtgaa atcaggaaag
    10561 atcattgtca ataactctga tatctctaca tacagttcaa aagaacttgc aaaaaaaatt
    10621 gcattattag cacaaactag tgaacaccct acaggtatga cagtagaaga agtagtatct
    10681 tacggacgat acccttatca aaaactgttc agtggcatgg acacgcaaga tcatgaagca
    10741 attgagtggg cgttggatgc tacaggtcta gctggatttc gtatgcggga tatcgcaacg
    10801 ctgtctggtg gacaaaccca gcgtgtctgg atcgctatgg cgcttgctca ggaaacagat
    10861 atcttgatct tggatgaacc gacaacattt ttagatccgg cacaccaatt agagatccta
    10921 cagttattga ctgaaatcaa tcaaatgaaa caaacaacga tcttgatgtc gattcatgac
    10981 attaatcatg cttcacgttt ttcagattat ttattagcaa tgaaaaacgg taagttgatc
    11041 acagaaggta cacctgaaga agtgatcaca aaagagtgta tgaaaaaaat atttgagatt
    11101 gatgcaacga ttgtcacgct tccaagaaca aataaaccag tttttctaac ctatgatttg
    11161 ttgccaaaag agcaggagga tcaattatga ggactatgaa gaaaaataga ctcctgcttt
    11221 tggtctgttt tgttcttttg attatttttt ttgttgctct ttcaaatggc gcagcgactg
    11281 tgggaaaagc agatttttgg caggcgttgt ttgattttga tagatcaaaa caaagtcacc
    11341 aaatcatacg aaatattcgt ttgcctcgtg tggcgggagc atttcttgta gggagttgtt
    11401 ttgcattgag cggtgcattg atgcaaggag tgacaagaaa tcctttagca gattcaggct
    11461 tactaggaat caacgcgggt gcttcgttgg caatggccct tagttttgct ttttggccta
    11521 aagcttctga tttttctgtg tttctaatgt cattggcagg ttcttttgct gtaactttgc
    11581 tcgtatttgg ttttttgcag ttttcgaaaa tgggcatgaa tcctttacag ctgattttag
    11641 ctggcgtagc tattagttct ttttttactg ctatcagcca agcacttacc caattcttta
    11701 atctgcagca ggatcttgct ttttggtttg taggcggagc tgccaatatc acatggaaac
    11761 aattagcagt attgtttcct ttatatatac ttgctgtttt ggtttctttt tcattaggaa
    11821 aagcaatatc tttgattcat ttgagtgaaa cacatgcatt agctcttgga agtcatccta
    11881 aacgaacgcg gttgattgtt ttctgtctag ttgcagtgtt agcagccatt gccgtttcgt
    11941 tagtcggacc tgtttcattt attgggctgt tggttccgcc agttgttcgc ggaatggttg
    12001 gaacggacta tcgttatgtt ctacctgtct cttttttagt tggcggtgga ttggttatgt
    12061 tggcggattt tacagcgagg atgatcaatc ccccttttga aacacctttt ggattagtca
    12121 ttgcattgat tggcgttccg tttttactga tacaagtaag gagggaacaa cggtgaaaaa
    12181 aatatattgg acgttctatt tcttgctttt tgtacttgtc atcctgtcta taggtagttt
    12241 aatgattggt acgcctttta tttacctgga tcagcttttt gccacgttca tagggaaaga
    12301 atcatcttct ttggcgctga ttgttagaga atttcgagtt cctcgattac tgatcagttt
    12361 attagcagga gcttgtcttg gcgtttctgg ttatatcttg caaggagtaa caagaaatga
    12421 attggcagat tctagtatat tgggcattaa tgccggagca ggatttttag tgatgcttta
    12481 tttaggtttc ttttcgcact attcgctgcc gtttctattg ctagttgtag cctttctagg
    12541 tggtgtttta gctgcttgct gtgtctatct tgcggcctat gatcgaaatg gctttttggg
    12601 tatgaatcga atcttattgt ccggtattgc cgttaatgcc ggcctttctg cattaacatt
    12661 gctatgcacg atccaattat ctaaggaaaa ttatggattt gtgaatgcat ggttagcggg
    12721 atcaatctgg ggtgcgagct ggccttatgt ttgggcattg cttccatggg cggttatctt
    12781 aattccgcta ggaggacttt atgcacagag gattgtattg ctatcatttg gtcaagaacg
    12841 cgcgcaagga ttaggcctta acgtggctaa aagtcaaaaa atccttttgt tgttagctgt
    12901 tgctttggca agcggtagtg tagcggtgac aggtagttta tcttttgtag gactactagc
    12961 tcctcatgct gcgaagctag tcgtacgtaa agaaaataac tggacctttg ttctgagcgg
    13021 tttgttcggt tccatttttc tgcttagtgc agatatggtt gctagagtga ttctactaag
    13081 tggggaagta cctacaggaa ttttgattgc cctttttggt gcgccatatt ttctgtatgc
    13141 actttttatc agacaaaaac acgcgctgaa cagttgaaat tcattaaaaa aggaaggcca
    13201 atcatttcta ggtcttcttt ttattctatt tatgcattta tttgaaagtt cgattgctat
    13261 ttttcttttt ttcctatacc attttatgag agaaggaagg gtgagttttt ggaccaattg
    13321 aaagtaagat tacgccggaa aggaatcgaa aagctgaaaa atctggccaa aagtggcaag
    13381 aaaaaggaaa aagaacagcg aatctataaa cagctattcg ccagcgagta ctggaagtca
    13441 gcaaaagtga tagggacgac tttatcgaat gaatttgaac tggatacaca gccggttatc
    13501 caacgagcag aaaaagaaca aaagaaaata gttgtaccta aaactttgcc gaaacgtcag
    13561 atggcgtttt atgaaatgga tgaacagtcc gttcttgtac gttcgagttt tggtgttctt
    13621 gagccacaat cagagaagct ctatttgccg gagatgatcg atctcctcat tgtaccaggt
    13681 attatctaca atagagccgg ttatcgaatc ggttttggcg gtggttatta cgaccggtat
    13741 ttaaataatt ttcataaaaa aacatgtagc cttgtttttc gtgaacaatt gaatgaacaa
    13801 tggcaaccgg agagctttga tcagaagatc caacaattgt ttattgacta aaatgaaatg
    13861 ggaggttttc atgaattatc aacagcaaat aaaattgcgt gcatggttaa gacgaccgtt
    13921 atggacctat gcttttttag ggatacagac agcggtcttc ctcatgatgg aattatttcc
    13981 gcgacttgag atcccttatt atgccggaat gtatggtcct tatcttgttc acttcaatga
    14041 atggtggcgt ttagtgacac caatcttcat ccattttgga ctgatgcatt tcgtaatgaa
    14101 ctcactgata ctgtatttta tggggcagca aatcgaggca atctatggcc attggcgttt
    14161 tttcttgatt tatatgttca gcgggataat gggaaataca gcaagctttg cttttaacga
    14221 agcgaatgtt ctgtctggtg gtgcaagtac ttcgatattc ggtttgttcg gtgcgttgtt
    14281 tatcttaggg tttcatttta aacataaccc agcgatccag cagctagtca gacattttct
    14341 tttatttatt gtgttgacat ttgtttttgg tcttctggat acctctgtag atgtttgggg
    14401 acatgtcggt gggctaattg gcggattagt gttagggaat gttttgggtc taccgaaaca
    14461 acagacaagc tattcgatac accaaaggat tctttctaca ctagtttttg tatttctttt
    14521 tgtaatatgt attttactgg gtttaaaaaa atatggttta cttgtataat atgaaggaag
    14581 tgcttaaaga tggagagctt atatgatgta cagcagctgt tcaaaaaatt tggcatcttt
    14641 atctatgtgg gtgcacgcat ctatgacatt gaattaatga ctatcgaatt gcgtaatctg
    14701 tatgatagcc gtttgattga tcatgataca tacatgacag cgtggcgcat attaaaaaga
    14761 gagcaccgaa ttgaagaaag ccgaaagaaa ggaaatttgt aatggacaag aagattattg
    14821 ggattgattt aggtggaaca acagcaaaat ttgcaatttt aacaccagaa ggagagattc
    14881 agcaaaaatg gagcatcgat accaatattt tggatgatgg taagcatatc gttcctgaaa
    14941 tcattgaatc aatcaatcat cgtttgaatt tatataatat gaaagccgaa gactttatcg
    15001 gaatcggcat gggtacccca ggaagcgtgg acagcgaagc tggaacggtt attggtgcgt
    15061 ataacttaaa ctggacagaa gttcaatttg tgaaaaaact gatcgaggca ggaacaggaa
    15121 tcaaattcgc tatcgataat gacgcgaatg ttgctgcttt aggtgaacga tggaaaggtg
    15181 ctggagaaaa cgatccagac gttgtattcg ttactctagg aacaggtgtt ggcggaggta
    15241 tcgttgctgg cggaaatttg attcacggcg tgagcggagc tggaggagaa atcggacata
    15301 tcactgtgga tcctgaaggg ttcgaatgta cttgcggtaa acgcggatgt ttagaaactg
    15361 tatctagcgc tacaggtgtg gtacgcctag gtcgtttctt ggcagaagaa tacgcagggg
    15421 attcaaaact gaaagcaatg ctagacaacg gagaagaagt aacaagtaaa gatatctttg
    15481 aaatggctca agaagacgat ccgtttgcat tgatggttgt tgatcgcgta tgcttctact
    15541 taggtttagc ttgcggaaac ttggggaata cattaaatcc gtcaagtatc gtgttaggtg
    15601 gaggagtatc ggcagcagga gaatttttac gtagccgtgt agagaaatat tttaaagaat
    15661 ttactttccc gcaagtaaga gaatcaacaa aaatcaaatt agcagagctt ggtaatgaag
    15721 ctggtgtcat tggtgctgcc tcattagcct tgaaatttgc ttaaaaaaac ggaggcatta
    15781 catggtttta tgggtaatta atatcatttt gctattgatt gtgcttgcaa tcatcctttg
    15841 ggaagtctat ctacgagtaa tggccaaacg ttcagcaacg actttgacag aagaagaatt
    15901 tcgagaagga atgagaaaag cacaagtaat cgatgttcgc gaaaaagatg tgtttgatgc
    15961 tgggcatatt ttaggtgcaa gaaacatccc ttacactgtt ttgaaagatt cattaggatc
    16021 tattcgaaaa gaccagcctg tctatattta cgatcaaaag aaaagtttga gcatccgtac
    16081 agcaaataaa ttacgtaaag caggttatca agagatttat ttacttaagg gtggatatga
    16141 cggatggtct ggaaaaatca aaagtaaaaa agtataaaac agatgattgt atgacagctt
    16201 taaaaaccga atcaatcatt agattttgac caagatcaca tgaacggcgc aatggatagt
    16261 tgtgccgttc atttttttta ttttgtgcta ttatctacat atataagcta agcgaaaagg
    16321 tagaaagaag gaaacaacta tgatcaaatt aaaacgtgtt taccttgatg ctgaaaaaga
    16381 ggatggctat agaatactcg tggatagatt ctggccacga ggaatttcaa aagaaaaagc
    16441 gcagttagat ctgtggttga aagaaattgc acctagtaat gaagcacgca aaacttttga
    16501 ccatgtacca gaacgatttg cttcatttgc aaagaaatac cagcaagaat tgattttcga
    16561 agaagaaaag aagcaagcta tttttaaact cagtcaactt atgaaagaac atccagtcat
    16621 tacttttgtt tatggtgcaa aaaatgaaca tcagaaccat gctgttttat tgaaaaaatt
    16681 tatcgaaatg gaggcatgca aatgaaaata gggatcattg gtgccggacc tagaggatta
    16741 agtgtagcag aaaggattct ccataataat caggacagac attctcttga actttttctt
    16801 ttcgatccca atggtcctgg aggtcgagtt tggcggttga atcagccacc ggagttatta
    16861 atgaattctg tttctcaaca agtgacgctt tttacagatg aaacattgtc cagtggagga
    16921 ttggtaacac agggacccaa cttgtttcaa tggagtaaag gacctgctac agattatatc
    16981 gaacagacgg taaccaaaaa taagcaacta attatagaag aaatcaatcg tctggaaaaa
    17041 gatcagcaat ctactcgatg tttatacgga ctctatcaac gctggttttt ctcaaagttg
    17101 caagaagaat ttccagcttc tattcagttt gtttattctt tagtaaaaaa agtaagcaaa
    17161 agaaaagatg gttatctttt acagacagat cagcaaagct attctatcga tcagcttgtt
    17221 ttagcaagcg gtcactggga aaatgatgag acagaagaag aaagagtgtg ggtagactac
    17281 gcaaagaaaa atgttttact gtatcagctt ccttctaatc ctgcagatgt agatctttcg
    17341 aagataccgc ctaaagaaaa tgtcttttta agaggtctag gcttaagttt ttttgattat
    17401 gtcggattgt ttacagtatc aagaggaggg cgcttcgaga acgaaaaggg aaaactggtc
    17461 tatcatccat caggcaagga accgattgta tacagtggat caagaagggg gctcccttat
    17521 taccctcgag gaagaaatca aaagcaaggc ggagcaatgg catggccacg gttactaaca
    17581 aaagaaaacc tggagcaatg gcatcagtcg ggatttttga ctggcgaaac attttttgac
    17641 tatctgaaaa aagacgttga gttgttttat tacaagaaaa tcatcgaaga atatcacttg
    17701 ccgattccaa agaaaaattt tgagcatgat tttctctccc tctcttcaga ggagtgtctc
    17761 ggaaagcatc cagcactaat cccttataga tgggattggt cgtatttaga aactcctctt
    17821 gcctatcaga atgagccctt ttcagaagct agccgaatat ttattgaagg gcagataaaa
    17881 gaagcggaaa aaggcaattg cacaggagca tttgcttcaa catttgatgc attaaaagac
    17941 tggcgtgatc caatccggca ggctattgaa tggcacgttt tttctgctaa ggaatacaaa
    18001 gaaatactat ggggatggtt tactcatcta aatagctttt taacgatcgg tccgccaatc
    18061 attcggacga aagaattagc agcactgata gatgcgggta tttttcattt gatcgaaccg
    18121 ccatttgata ttcagcaaaa gcacaaccgc tatgttattt ctctacaaga taaagaaatc
    18181 agcagtcgct atttgattga agctcgattg ccacaaacaa gcttttctcg ttcaagtaat
    18241 cctgtcataa aaaatttgaa ggaaaacaaa cttgttcgcc ctttttcatt ttcagataat
    18301 acaggaagtt atcattcaga agctatagat gttgagcgag gtacgaatcg cgtgattgat
    18361 gcttcagggg aaatccagtc tgacttttat tgctatggaa tacctgttga aggtgtggat
    18421 tggctaactg cagcagtagc tagaccttat acagatccat ggaatctgag acaggcagat
    18481 aaaatagcac aacttatatt gactgacaaa taaaaatacg catgatgact tatctgtttt
    18541 ttagaaagtc ttcatgcgta tttttaaa
//
LOCUS       DAJSMI010000042        18510 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJSMI010000042 DAJSMI010000000
VERSION     DAJSMI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.042"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            81..515
                     /locus_tag="ON430_001164"
     CDS             81..515
                     /locus_tag="ON430_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3219090.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(593..1126)
                     /locus_tag="ON430_001165"
     CDS             complement(593..1126)
                     /locus_tag="ON430_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCR3219091.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1220..2398)
                     /gene="mutY"
                     /locus_tag="ON430_001166"
     CDS             complement(1220..2398)
                     /gene="mutY"
                     /locus_tag="ON430_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR3219092.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2391..3188)
                     /gene="recX"
                     /locus_tag="ON430_001167"
     CDS             complement(2391..3188)
                     /gene="recX"
                     /locus_tag="ON430_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR3219093.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3295..4677
                     /gene="rlmD"
                     /locus_tag="ON430_001168"
     CDS             3295..4677
                     /gene="rlmD"
                     /locus_tag="ON430_001168"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR3219094.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4851..5429)
                     /locus_tag="ON430_001169"
     CDS             complement(4851..5429)
                     /locus_tag="ON430_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCR3219095.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5751..6221
                     /locus_tag="ON430_001170"
     CDS             5751..6221
                     /locus_tag="ON430_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCR3219096.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6329..7555)
                     /locus_tag="ON430_001171"
     CDS             complement(6329..7555)
                     /locus_tag="ON430_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3219097.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8309..8509)
                     /locus_tag="ON430_001172"
     CDS             complement(8309..8509)
                     /locus_tag="ON430_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3219098.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8643..9251)
                     /locus_tag="ON430_001173"
     CDS             complement(8643..9251)
                     /locus_tag="ON430_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCR3219099.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9336..9806)
                     /locus_tag="ON430_001174"
     CDS             complement(9336..9806)
                     /locus_tag="ON430_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR3219100.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9854..10768)
                     /locus_tag="ON430_001175"
     CDS             complement(9854..10768)
                     /locus_tag="ON430_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3219101.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10914..11717
                     /locus_tag="ON430_001176"
     CDS             10914..11717
                     /locus_tag="ON430_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCR3219102.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11895..12842
                     /locus_tag="ON430_001177"
     CDS             11895..12842
                     /locus_tag="ON430_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR3219103.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12910..13203)
                     /locus_tag="ON430_001178"
     CDS             complement(12910..13203)
                     /locus_tag="ON430_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCR3219104.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13231..13896)
                     /locus_tag="ON430_001179"
     CDS             complement(13231..13896)
                     /locus_tag="ON430_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCR3219105.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(14036..14668)
                     /locus_tag="ON430_001180"
     CDS             complement(14036..14668)
                     /locus_tag="ON430_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3219106.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14675..15742)
                     /locus_tag="ON430_001181"
     CDS             complement(14675..15742)
                     /locus_tag="ON430_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3219107.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15739..16470)
                     /locus_tag="ON430_001182"
     CDS             complement(15739..16470)
                     /locus_tag="ON430_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCR3219108.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16639..17118)
                     /gene="greA"
                     /locus_tag="ON430_001183"
     CDS             complement(16639..17118)
                     /gene="greA"
                     /locus_tag="ON430_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR3219109.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17254..18429)
                     /gene="mltG"
                     /locus_tag="ON430_001184"
     CDS             complement(17254..18429)
                     /gene="mltG"
                     /locus_tag="ON430_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCR3219110.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5504 a         3740 c         2979 g         6287 t
ORIGIN      
        1 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
       61 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
      121 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
      181 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
      241 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
      301 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
      361 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
      421 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
      481 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
      541 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
      601 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
      661 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
      721 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
      781 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
      841 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
      901 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
      961 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     1021 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     1081 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     1141 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     1201 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     1261 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     1321 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     1381 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     1441 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     1501 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     1561 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     1621 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     1681 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     1741 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     1801 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     1861 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     1921 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     1981 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     2041 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     2101 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     2161 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     2221 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     2281 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     2341 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     2401 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     2461 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     2521 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     2581 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     2641 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     2701 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     2761 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     2821 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     2881 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     2941 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     3001 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     3061 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     3121 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     3181 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     3241 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     3301 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     3361 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     3421 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     3481 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     3541 ggtggctgtc aattacaaca cttagcttat caagctcaat tggattttaa aaaggatctg
     3601 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     3661 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     3721 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     3781 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     3841 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     3901 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     3961 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     4021 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     4081 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     4141 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     4201 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     4261 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     4321 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     4381 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     4441 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     4501 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     4561 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     4621 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     4681 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
     4741 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
     4801 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
     4861 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
     4921 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
     4981 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
     5041 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
     5101 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
     5161 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
     5221 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
     5281 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
     5341 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
     5401 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
     5461 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
     5521 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
     5581 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
     5641 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
     5701 ttgctgtgag ccttcatggt aaactaatat tatagaaagg agttagtaaa atgaagatga
     5761 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
     5821 ggttgcttag cggaaatccc ggaaaatatt actattcttt gcaacttcca ccatttgcac
     5881 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
     5941 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
     6001 tgattcagtt catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt
     6061 tcattgcagc aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt
     6121 tgtggctgca tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat
     6181 tcgcaactct attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc
     6241 tgtaatttaa cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg
     6301 cactctttat taaccaaact atttttttct agtacccact tgttgttcca tactcattat
     6361 tctggtcata accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat
     6421 cttgacttgg tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat
     6481 cttggtactg atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat
     6541 tcgatccatc accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag
     6601 cattctcatt tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca
     6661 gttcattttg gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga
     6721 tcatttgtcc ttcgccttgc aactggtctt gtttgattgt ttcaaacgcc ggggtgtagt
     6781 tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg
     6841 cttttaaaat ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt
     6901 tcgtcactac ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc
     6961 gcattcgaga ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat
     7021 ggacaccatc caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga
     7081 tcaaatcttc catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca
     7141 gttgctcaat tgaatccatg gccatttcga caccaccaaa tgcgtaggca tgatttaatt
     7201 tatctaccgt tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg
     7261 ttgatttttt ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt
     7321 ccgttcttcc taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt
     7381 catcaatgct tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt
     7441 tgtttactgt ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa
     7501 tcagcaaaac gaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac
     7561 acctctgttt cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac
     7621 tttttaatca taagaaaact ttaaaaaaca tttttgtgta acgattatta ttttttaaaa
     7681 tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac
     7741 gcagttcgca atcgttttat ctttttttat gtattttttc cagaaaaagc gaacaacaag
     7801 gtattttcca aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca
     7861 gtctttgcct accgttcaga ttatcattcg atttcacgac aatttcttcc tatgactggt
     7921 ccttttgtta aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa
     7981 tgactcgaac tagtgattgc attaccatca tcttttaatg gatagatatg ctcctcttca
     8041 aagtaaaacg attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag
     8101 tggtttcaca aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt
     8161 actaacgaag aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg
     8221 catttactta gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta
     8281 agaagtcgtg tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc
     8341 cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc
     8401 cgtctccttg aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa
     8461 acccaaatcc tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatatc
     8521 ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct
     8581 agcaagcttt cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat
     8641 ggttattttg ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat
     8701 gctgcaatat aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg
     8761 tccaaaccta gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag
     8821 gtaatagcta attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca
     8881 gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc
     8941 gcttctttta tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa
     9001 aatgaatggt tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga
     9061 atagcgtcca tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc
     9121 tcaattgctg aatttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc
     9181 atcgtttctt cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat
     9241 gtgtaagtca taaaaaaatt cctcctcaat gttttcttaa taatgtctac attacaaaga
     9301 gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct
     9361 acttcttctt caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc
     9421 gcaaggataa caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca
     9481 ttgatcaaca ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag
     9541 aacttaccgc attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa
     9601 agtttgttcg ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag
     9661 gccatactta aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta
     9721 gccaaaccac caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat
     9781 ccttttaact gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat
     9841 catactctat atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct
     9901 ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag
     9961 aaattatacg aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat
    10021 gtccgtcgaa aataattatc ttttcgctat actgccaaat atggacatga tgaacatcct
    10081 ttaccccatc gattgcttca atttcttgtt ccattttttc atagtcgata gcaggtgcac
    10141 tttgtatcaa aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata
    10201 tcgaaatgat cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa
    10261 ttgctccgat aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag
    10321 attttatatt caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg
    10381 cgagcaatcc aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag
    10441 tgaaccgctt gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt
    10501 tgatgaaagc actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt
    10561 cttttcgggc cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt
    10621 tatggaaagc atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg
    10681 ataaagtgat caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg
    10741 gctgtgtagg cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa
    10801 taacattttt ttgatatttc accaatcatt cagctgtttc cttaaagcac ttgtatttca
    10861 cttgatactt cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa
    10921 caaaacaatt aattatcagt tcctttatac gattcaaaga cctaaaggag gcgagaaatg
    10981 tcccattttg gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa
    11041 tctcttttca agtttttcaa gtcttaaaca acattcaatt ggatgggcaa aagattgcta
    11101 ctcgcattcc tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat
    11161 tcatttacca aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac
    11221 aggacatctc tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac
    11281 ttatcatagc tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag
    11341 aacttcccta caccaatgaa tctttaaaaa acttaacagg taagcagctc cgttcttttt
    11401 taagtgaagc atctatacca ctttggataa aagtcctgac atttatattt tctatttatc
    11461 ctagttttct gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg
    11521 cacgctttcg actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa
    11581 tttcattgcc tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacta
    11641 gcgccttcat tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa
    11701 aaatatcgat caattaagaa ataaagacaa ctcgttattt ctacttcaat agaccctttt
    11761 agaggttgtg acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca
    11821 aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag
    11881 gcgggaaatt aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat
    11941 ttatcgaaga aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag
    12001 aggatctaca aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat
    12061 tagcctcttc taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc
    12121 ttgagacttt ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat
    12181 cgattacgga gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac
    12241 aaaaggcaca gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag
    12301 ggaaaaaaat actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca
    12361 aagcattaga catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg
    12421 aaaagacttt ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca
    12481 atatcttacc tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact
    12541 ttgatccaaa atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc
    12601 tggtgcaggc tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag
    12661 agcctcttcc acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca
    12721 ttgcaggttt gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga
    12781 aaagctactt tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt
    12841 aatagctgaa aacaatattt tcaaaagaaa aaagagaagg tgacaaaact tgtgtcaccc
    12901 tctctttttt tattgttttt taaagtgata ggttggctcc tccaggttgg gatcataatc
    12961 aacaagtaaa tcatatcctt taaagaacca ttcatctgct tcctcgataa aaaaaagtgt
    13021 cccatcaatt tccactttct ttacaggatg ttctggctgt tcaactgaca ttccaatcga
    13081 aaaaccatca tgaacctctg ttttgccgta gacttttcca aaaaaacgta ttccgtaagt
    13141 ttctggcaga tcaatttctg agtcaaacca cttttgtgct tttggcgtta ccgttaattt
    13201 catcccaaca acctactttc tcttttcttt ctattgattc atcttatcgt ctagaatatc
    13261 cacatcgcct gtctctccaa taaccagtag attatcattt tcacgaacat attcatctgc
    13321 tgcaggagaa gcgataaccg tttgatctac tcttcgtatg gcgactactg ttaagtcaaa
    13381 acgttgcctg aaatttaatt cagctaatgt tttgttgaaa aatttaggat tggagactcg
    13441 gatttctgct aaagaatatt tatctgacaa ctctaggtaa tctaaaatat tctttgacac
    13501 taaattatgc gcaatcctta ttcccatgtc tcgctcggga tggacgacgc gatcagctcc
    13561 gatcttttca agtactctgg catgatactc attttgggct tttgctaaaa tatttggtac
    13621 gcccatttcc tttaccatca gagtaactaa gatactggct tgaatatctt ctccaatagc
    13681 gacaattaca tgatcgaaat ttcttatgcc taatgatcga agtgtcattt catcctgtgc
    13741 atttgccaca acagcatgag tggctatatt catatattca ttgacccgat cttcattact
    13801 atcaatagcc agtacttctt gaccagcttc gatcagtgtt cggcagatac ttccgccaaa
    13861 tcgtccaaga cctacaattg caaaattttg tttcaccgtt ttcctccttt agtatcttct
    13921 tttctcagta taacaaagcc tttacaaaaa caaaagcaag catcattatt gtaaaaaagc
    13981 caagaaaaat aatagttatt ttctcagctt ttgattatcc tctttatcat cgtttttatt
    14041 tcaccaaacc atgtttgaat gcatagatag cggcttgtgt acggtcttcc acttctagct
    14101 tagccaatat attggaaaca tgtgttttta ctgttttcag tgtaataaac aattcgtccg
    14161 ctatttcctg attacttttt ccttcagaaa tcagcattaa aatttcattt tcccgattcg
    14221 ttagttcttc atgtaataca ggctcatttc gccgactcat tttttccatc atttttgttg
    14281 tcacttctgg ttctaatact cgctcgccac gttgcgttgc ccttatagca tcagcaattt
    14341 catgtgcggt agaggtcttc aacaaatacc ctgctgcacc tgcttcaatg gcaggataaa
    14401 ctttttcgtc atcaataaaa ctggttacga taatgatttt agcttctggc caatctttta
    14461 agattgcttt tgttgattcg ataccatcca tttcttccat gactaagtcc attaaaatga
    14521 cgtccggtcg aagtgtcatc gccttctcat agccttgtcg tccattttca gcttctccaa
    14581 ttacttcaat gtcttcttga atagataaat aagaagagac gcctaagcgc accatttcat
    14641 ggtcatctac taataaaact tttatcatcc ttatctatgc ctccttcatc aaagggacct
    14701 tgatttctac acttgtacct tgacctttaa aactaatgat ttttactgtt ccaccaatac
    14761 cagctactcg ttctttgata ttgttcaatc cataacttcc cgttttcgtt tcattcatat
    14821 taaaccctgt tccgtcgtca ataatacgta acaaaagatt attgtctatt ttgtgcaaat
    14881 atacctctaa ttcattggct ttagcatgtc ttaatgtatt tgataacaat tcttgaacga
    14941 ttcggaacaa gtgatcctca atggaactag atagttttac atcttctaca tcccatttca
    15001 gcgaaatctg gattttattt tgcaattcct tcaataactg ctcgatacct tgctttaaac
    15061 ttttctcttc taaattgact ggacgtagat gaagaagcag cgcacgcatc tcagactggg
    15121 aagcgttgat gatctctgct accattttca actgcttgcg aaacatttct ggtgtctcgc
    15181 ttttttctgc ttgctccgtc aatgccgaca tcatcatcat tgctgcaaac aattgttgcg
    15241 aaactgaatc atgcagctca cgggctaatc gatgtctttc taattctaaa atttcttctt
    15301 tggtttgtcc atctacatat tggggacggc tcgttacaat ttgtaattca ctagatactt
    15361 caatcatttt ttcttttatc ttagtgattt ccttatcaat atctttgatg tataaatcgt
    15421 ccgatgcact tgggatcgga agaagcaaca atttggattc ataattgcca gaagaaagtg
    15481 cacgcagctt ttcttcgatt ttcccgtatt ttgtcttttg aataagtgat aataatagaa
    15541 agacaattaa tcctacacct aaggaaatgg ctaacaaatg gaagataagc ggaacatata
    15601 ataaacgtgc acgcagtaat tctccccacc aatgactttg attgctggca taaaaataag
    15661 tgaaaagtga aaatagaata ataaggaaag ccgcaatccc cgaataaaca gctagcatcg
    15721 ctctggatat ttttcccatc atattcgtat cacctcgaca tctccaatca aggtattagt
    15781 aataatcttc aatctacgag ggttttcatc ataatcactg ctgtagattt tgatttgttc
    15841 atttttcaaa gaaaattgct cttcttcaaa caagacattt cctactaatg tcgcatgctc
    15901 taatcggatg gctacgccca aaggtactaa gatccgtgtt ctgcctatcc cttttcttac
    15961 aatcacaata ttatcatctt ttggcaaaag cgtattgccc aaatctatga tagtatcacc
    16021 agaaatgacg gcgatgttga tatcgtccca ttcatagacg tcgtttccaa tacgttggtt
    16081 accgaataat tgctgtcttt tccgctcatt attatgtgtt tttaattgct ctgtttggat
    16141 catcatgatc tgtttctttc gccaaaaagc atttttggtt aaatctatcc ctgaaatctc
    16201 tacacctttc agaccaataa ataaaacggc aaatacagcc atcatccata aagcaggact
    16261 gttgactaag ctaaaaaaaa tgaccaaact tcctaatatc aattgaaaat tttgaaattt
    16321 agtacgagga tgtttgcgca tagctagata tatattgaaa ataccgaata tcactaaaag
    16381 caaaagtcca gtattgttta cgatttgcca aacagcaaat aacagcagta gtgcttcaac
    16441 taccacaaaa aaacgccaag aactattcat cgctctttct ccctcctgac acattctcat
    16501 ttcatcatac ctattttttt aactgttggc tataggactt tagatgtaag atcgtttgtt
    16561 gaaatctaaa atacacaaag agaaaaagat ctagagtgta tcatcagtaa gatgaactct
    16621 ctagatcttt cagtcagact acgcttgttc tactttgacg attttaacta acatatcgcc
    16681 acctggagta gcaatagtta cttcatcatt tttacgtttg ccaatcaatg cttgcgcaat
    16741 aggagaatca ttggaaattt tgccagaaaa cggatctgct tcagcgcttc cgacaatcgt
    16801 gtactcttct tcttctccat ctggtagttc gatgaatgta actgttttac caattgatac
    16861 ctcatctgca tctacaccgt cgttatcgat gatttgagca aaacgaatca tattttcaat
    16921 tgttgtgatt cttccttcaa caaatgcttg ttcatctttc gctgattcat attcagagtt
    16981 ttcggaaaga tctccgaaac cgcgggcaat tttgattcgt tcaatgattt ctccgcgttt
    17041 gactgttttt agttcttcta attctttttc taatttttct ttaccttcta gagtcattgg
    17101 ataaactttc tctaccatgt gtaacgtctc cttttttcga taaaataccg caaaaggtaa
    17161 tcttttgagg aatatagtcc aaaaaagatt accacgcttt tcttcaaatg taaatgtttt
    17221 tctaaaaatg atcaatgtct agagcctgtt tttctaagaa ttatttacat atttttccac
    17281 taattccata tgttcttcat aagttttagc gaaataaaca tttccagtgc tcgtatctgc
    17341 tacgaaataa taatagtcat tttctgctgg ttctaatact gccttgattg cctcttcgct
    17401 tggattgtca aaaggtccag gtccatagcc ggtattggta tacaaattat atggtgaatc
    17461 tacagctgta tcttcatacg tcactaattc tttgtgttca cctaatgcgt ataaaacaga
    17521 gatatctgac tgaagtggca tatttgcttt gattcggtta aagaagactt gtgcaatgtt
    17581 tttacgatca ttttctttta cgccttcttt ttcaactaga gaagctagag ttaaaacttc
    17641 ttgtacagtc aaatttttct gcttgatagc tgaataatag ttttgcatga ccgtattcgt
    17701 cttgttcacc atttcaatca ctagatcctt aagcgaagta ctcgtgtaat aatcatatgt
    17761 ggctgggaat aagtaacctt ccaaacggta ttttgtgttc tcagctttag atgcactttc
    17821 caacagttct gggaattttt ggtgcaactc gttaaagaaa gtctcatcat tcactaaatc
    17881 taaaaattct ttcttgtctt taccagttac cttcgctacc cgttcagcaa tctgatcgat
    17941 atcgtaccct tctggaatgg caattttccc atctgcaact tttgttggct cagaagtacc
    18001 gccttcttgc aactgtttgc caatctcgtc aagagtcata ttaggcgcta actgataata
    18061 gccagcttga aagccagtta aatttttaaa ttttgtataa taattgaaaa caatcccgct
    18121 tttaataata ttgtccttct ccaaaatctc accaatctgc ttgttagaag agccactcgg
    18181 gatctcgact tggactaatt ggtcatctga tttatctaaa ggcttcaacc cgctatctac
    18241 ataacgatac acggtaaatc caagaaatcc gccaataatc aataatgtaa gagcaatcac
    18301 taaaatgatc ttcctcacaa tcttatcttc ttttttacgc agatttctcc gcttgtcgat
    18361 tatatctgct gtagctgcct tactggcttc ttttttatct ttacttgggt gttgtttgtt
    18421 tcctgacaaa acaactcctc cttactatca agttcgtaac tttattcatt atacatgata
    18481 tttgaattgg cgtgaatgat tttatttaaa
//
LOCUS       DAJSMI010000043        18504 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJSMI010000043 DAJSMI010000000
VERSION     DAJSMI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.043"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            99..746
                     /locus_tag="ON430_001185"
     CDS             99..746
                     /locus_tag="ON430_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR3219111.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ON430_001186"
     CDS             1139..3784
                     /locus_tag="ON430_001186"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR3219112.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ON430_001187"
     CDS             4094..5407
                     /locus_tag="ON430_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR3219113.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ON430_001188"
     CDS             5397..6050
                     /locus_tag="ON430_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3219114.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ON430_001189"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ON430_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR3219115.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ON430_001190"
     CDS             6947..7147
                     /locus_tag="ON430_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3219116.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ON430_001191"
     CDS             complement(7401..9080)
                     /locus_tag="ON430_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR3219117.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ON430_001192"
     CDS             complement(9082..9294)
                     /locus_tag="ON430_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCR3219118.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ON430_001193"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ON430_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCR3219119.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ON430_001194"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ON430_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCR3219120.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ON430_001195"
     CDS             13269..13466
                     /locus_tag="ON430_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219121.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ON430_001196"
     CDS             13619..14104
                     /locus_tag="ON430_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR3219122.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ON430_001197"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ON430_001197"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR3219123.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ON430_001198"
     CDS             14981..15508
                     /locus_tag="ON430_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219124.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ON430_001199"
     CDS             15599..16336
                     /locus_tag="ON430_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR3219125.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ON430_001200"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ON430_001200"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR3219126.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ON430_001201"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ON430_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR3219127.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3200 c         4022 g         5298 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAJSMI010000044        18345 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJSMI010000044 DAJSMI010000000
VERSION     DAJSMI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.044"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..146
                     /locus_tag="ON430_001202"
     CDS             <1..146
                     /locus_tag="ON430_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3219128.1"
                     /translation="RYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPILE
                     KEI"
     gene            complement(183..866)
                     /locus_tag="ON430_001203"
     CDS             complement(183..866)
                     /locus_tag="ON430_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219129.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            1070..3106
                     /locus_tag="ON430_001204"
     CDS             1070..3106
                     /locus_tag="ON430_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCR3219130.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEQPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYKVTRVSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFGE
                     DLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFANNGTLVYP
                     KLIADKETKDKKNVIGETALQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEIK
                     EKQDVKGKENSFLFAFNPDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3215..4366)
                     /locus_tag="ON430_001205"
     CDS             complement(3215..4366)
                     /locus_tag="ON430_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCR3219131.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4520..5050)
                     /locus_tag="ON430_001206"
     CDS             complement(4520..5050)
                     /locus_tag="ON430_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR3219132.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5149..5499)
                     /locus_tag="ON430_001207"
     CDS             complement(5149..5499)
                     /locus_tag="ON430_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCR3219133.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5656..7185)
                     /gene="ahpF"
                     /locus_tag="ON430_001208"
     CDS             complement(5656..7185)
                     /gene="ahpF"
                     /locus_tag="ON430_001208"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR3219134.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7195..7758)
                     /gene="ahpC"
                     /locus_tag="ON430_001209"
     CDS             complement(7195..7758)
                     /gene="ahpC"
                     /locus_tag="ON430_001209"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCR3219135.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7902..8564)
                     /locus_tag="ON430_001210"
     CDS             complement(7902..8564)
                     /locus_tag="ON430_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3219136.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8748..10394
                     /locus_tag="ON430_001211"
     CDS             8748..10394
                     /locus_tag="ON430_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR3219137.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10385..11467
                     /gene="uxuA"
                     /locus_tag="ON430_001212"
     CDS             10385..11467
                     /gene="uxuA"
                     /locus_tag="ON430_001212"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3219138.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11575..13128)
                     /locus_tag="ON430_001213"
     CDS             complement(11575..13128)
                     /locus_tag="ON430_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3219139.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            13276..14565
                     /locus_tag="ON430_001214"
     CDS             13276..14565
                     /locus_tag="ON430_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3219140.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(14691..15770)
                     /locus_tag="ON430_001215"
     CDS             complement(14691..15770)
                     /locus_tag="ON430_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3219141.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15879..16319)
                     /locus_tag="ON430_001216"
     CDS             complement(15879..16319)
                     /locus_tag="ON430_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3219142.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16376..16786)
                     /locus_tag="ON430_001217"
     CDS             complement(16376..16786)
                     /locus_tag="ON430_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3219143.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16967..17401)
                     /locus_tag="ON430_001218"
     CDS             complement(16967..17401)
                     /locus_tag="ON430_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3219144.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17501..18328)
                     /locus_tag="ON430_001219"
     CDS             complement(17501..18328)
                     /locus_tag="ON430_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3219145.1"
                     /translation="MWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGILLEAVLG
                     FDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITSDRLLSVI
                     FGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSLNLFVFG
                     IEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIGRGLTLLE
                     AKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFTYHPIE"
BASE COUNT         6319 a         3413 c         3278 g         5335 t
ORIGIN      
        1 aacgttattt acgtttgact gtttatgaaa aaaatcatgc aggtattcgt ttttatgaac
       61 gtcatggatt taaaaaaatc gggatcaaac attttccttt aggaaaacaa gatcgtattt
      121 gtccgatttt agaaaaagaa atctaaaaaa acgtcctcac ttttgatgct gaggacgttt
      181 ttttagactg gtaatggaac cataattcgc tttccttttt gataaatcgc taattccgtc
      241 acaccatttg cttttaacag agcaaaaaga tcatcgaaat ggaaacggaa atgttcaagg
      301 taatgcccat ctgatccaac actaaacaag gtaccaccac gtgaaagata taaatcgatg
      361 acatattcat ataacgctaa attgtcatac aagtaagcgc tcttagcatt taattcgaat
      421 gccaatttat ttttgattac ttgatctaga atcggtaaaa actgcgcttc atattgttta
      481 aattcagcaa cggatagacc aaatacccga aaaccatagt caaaatgagc aaagacatct
      541 gcttctataa caactagtgc ttctgaaagt tgtgcaaaat actgaggaat cacaatagca
      601 ggatccatat ctttgacttc gtcatctaaa taatcaaatt gcccattatg atgcacactg
      661 agtaatttta aatcgtaatc tttatcggct aaatagtcga tgatccgatc cttttcagaa
      721 gcgatgtaac cgatttcgat gccttttaaa acacgattgc caaaacgctt atttaagcta
      781 tcgattgttt gactgtacat ttcataatcg gggatatcat cgactttagt aacaggattg
      841 gctaagtcaa aatgttcagt tgtcacgacg taaccatcgt agtttgttaa atagtcctca
      901 tttcttggga gttttgagac tactatatgc gtttttttgt tgatttacca acatgtttag
      961 acattttcct ctaggaaaaa attaactagc accacgggtt ttattatgtt agaataaaca
     1021 ggtataaata gtgaaataaa ggaatgacaa gcaagagaag gaggaaaaaa tgaaaagaag
     1081 tgacaagcac ggcaaaaatc gaacaggcgc ttatattgcc ggcgcagtga tcttaatagc
     1141 agctgcgggt ggcggttatt tttactacca gcactatcaa gaaacccaag cagtagaagc
     1201 tggagaaaag acagttgagc aatttgtcca agctttgaac aagggagatt ataacaaagc
     1261 tgcagaaatg acatcaaaaa aggcggcaaa taaaagtgca ttatctgaaa aagagatctt
     1321 agataaatac cagaatatat acggtgctgc tgatgtcaaa ggacttcaga tatcgaatct
     1381 aaaagtagat aaaaaagacg attctactta cagtttttca tataaagcaa agatgaatac
     1441 ctcattaggt gaattgaaag atctttctta taaaggaaca ttagacagaa atgatggtca
     1501 aaccacgatc aactggcaac ccaacttggt ttttccagaa atggaaggaa atgacaaagt
     1561 aagcctgacc acacaagaag cagcaagagg gaatattata gatcgaaatg gtgaaccatt
     1621 agcaacaaca ggcaaactaa aacaattagg agtcgttcca agtaaacttg gagatggagg
     1681 cgaaaaaaca gccaatatca aagcgattgc ttcctccttc gacttaacag aagatgctat
     1741 caatcaggcg atttcacaaa gctgggtaca acccgattac tttgtcccat tgaaaatcat
     1801 tgatggagca acgccagaac ttccagctgg agctaccatt caagaagtag acggcagata
     1861 ttatcctttg ggtgaagcag ctgctcagct gattggttac gtgggagata tcacagcaga
     1921 agatattgat aagaatccag aattaagcag taatggaaaa atcggtcgct caggtttgga
     1981 gatggctttt gataaggatc ttcgtgggac aacaggtggg aaattaagca ttacagatgc
     2041 agacggtgta gagaaaaaag ttctgatcga acatgaagtt caaaatggca aagatatcaa
     2101 attgacaatc gatgcgaagg cacaaaaaac agcttttgac agtctaggag gaaaagctgg
     2161 atcaactgtt gcgacaacgc caaaaaccgg tgatcttctt gcgcttgcta gctctccaag
     2221 ctatgatcca aacaaaatga caaacgggat ctcacaagaa gattacaaag cttatgaaga
     2281 aaatcctgaa cagccattca tcagccgatt tgcgacaggt tatgctcctg gatctacgtt
     2341 taaaatgatt acagcagcaa tcggtctcga caacggcact atcgatccaa atgaagtgtt
     2401 gacgatcaac gggcttaaat ggcaaaaaga cagttcttgg ggatcttata aagtaacgcg
     2461 tgtcagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     2521 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     2581 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     2641 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     2701 aattaatcct atccagcaag cagcaatgta ttctgttttt gccaacaatg gcacacttgt
     2761 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcggcgaaac
     2821 agcattacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
     2881 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
     2941 agaaaaacag gatgtaaagg ggaaagagaa cagtttcttg tttgctttca atcctgataa
     3001 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
     3061 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
     3121 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
     3181 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
     3241 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
     3301 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
     3361 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
     3421 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     3481 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     3541 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     3601 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     3661 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     3721 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     3781 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     3841 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     3901 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     3961 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     4021 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     4081 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     4141 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
     4201 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     4261 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     4321 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     4381 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     4441 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
     4501 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
     4561 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
     4621 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
     4681 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
     4741 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
     4801 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
     4861 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
     4921 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
     4981 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
     5041 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
     5101 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
     5161 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
     5221 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
     5281 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
     5341 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
     5401 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
     5461 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
     5521 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
     5581 aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt
     5641 tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc
     5701 tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc
     5761 gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt
     5821 catttcgatc gttctatcta gccaatccgt attagggagt aagccgacta gaatgaatac
     5881 tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc
     5941 tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc
     6001 aagagagact agtttatcct gtaagatttg atctgctttt aattctggta agaattctag
     6061 aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc
     6121 accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata
     6181 agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc
     6241 acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt
     6301 aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg
     6361 gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt
     6421 accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac
     6481 aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc
     6541 accgccaatc actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg
     6601 gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc
     6661 atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat
     6721 cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc
     6781 ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt
     6841 ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg cccgtccact
     6901 aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa
     6961 aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc
     7021 tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag
     7081 tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag
     7141 tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat
     7201 ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg
     7261 acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc
     7321 actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg
     7381 atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat
     7441 acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc
     7501 tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc
     7561 aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca
     7621 aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt
     7681 ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt
     7741 tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat aattattgta
     7801 atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa
     7861 cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg
     7921 ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt
     7981 tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt
     8041 tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct
     8101 aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa
     8161 caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact
     8221 gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg
     8281 gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct
     8341 gcagaaacaa gattaatttt tgaaacatac gtaccacttt gaggtatcgt atacacgagt
     8401 tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt
     8461 tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt
     8521 ttacgaatcg cttgataagc ttgagattgc aagctgcttt tcatctttat tcctccaaaa
     8581 tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt
     8641 aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa
     8701 aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa
     8761 acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg
     8821 accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg
     8881 gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat
     8941 tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc
     9001 aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac
     9061 gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg
     9121 ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg
     9181 aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata
     9241 taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgcg ctactttata
     9301 aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa
     9361 acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt
     9421 atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga
     9481 cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag
     9541 agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg
     9601 tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga
     9661 aattcgaaaa agctggagta ttcttgactg atcgtgcaac ggtagataaa gcagatacga
     9721 tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac
     9781 tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg
     9841 aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga
     9901 tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct gatatgcctc
     9961 agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa
    10021 aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg
    10081 ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc
    10141 cagacccact attgacagac tttcagcata agctttccga tattcaactg ggtacgcaag
    10201 atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg
    10261 tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag
    10321 caggaccgaa tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt
    10381 tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga
    10441 aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg
    10501 agaggtatgg cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt
    10561 aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc
    10621 ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat
    10681 agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta
    10741 ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc
    10801 aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttgaattgc cgggatggga
    10861 acctgagcgc ttagctcata tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga
    10921 aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt
    10981 aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg gattacctcg
    11041 aatcgtaaag aatcgagaag atttagattg gttgtgccgc atagtagaca gcccttataa
    11101 cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat
    11161 tttagcagaa ttttgtcaaa gagatcgaat cccatttgca catgttcgaa atattaaatt
    11221 tttagctaat cgagatttct atgaggcacc gcataaatcc gaatacggtt cattagacat
    11281 gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca
    11341 tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt
    11401 aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga
    11461 taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga
    11521 tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga
    11581 agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat
    11641 atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa
    11701 aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat
    11761 gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc
    11821 tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac
    11881 aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg
    11941 cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat
    12001 ataaaattgt tcaatggcgg tttggacttc tttcaacacg ctatgttctt gataaagcat
    12061 gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt
    12121 tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac
    12181 agtacaaaag gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt
    12241 taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg
    12301 agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat
    12361 atctacagag ggatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac
    12421 gtctttcaca gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt
    12481 atctatcata ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat
    12541 aaaagccact acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt
    12601 ccaataaata tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct
    12661 gccattatta gaaaaggaga tagacaaacc gtacaatgct aaaaatgcat taatcttttt
    12721 cattttcgta tacaaataag aggcactata gtttgtttgc tgacttaaac tgataatcga
    12781 atttgtctga cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta
    12841 tcttccaaat agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa
    12901 aaaaggtttt tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc
    12961 taattctttg atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata
    13021 ttgtgtagta ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt
    13081 gctttttttc ttcaagagaa acgttttaat cgcttcttga gtttgcatga aagaggctcc
    13141 tttttactta aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa
    13201 aaaacagtaa acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca
    13261 aggagtgaga gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc
    13321 ttatcgctta ctataaatca gacagtccac acagaggaga tagaaacagc caaatggaca
    13381 gaaaccaagt gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat
    13441 tcaggagtcg caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat
    13501 tcaggagact tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccgct
    13561 ttgtcgattg gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc
    13621 ttcaccgtta cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca
    13681 gatggactac cgaacgatca tcatcattta acgccgtata gtcagcggca gacaattgag
    13741 caagattcag ctatcgactc aggaggagat cttgttgaag aaccaacaga taaaccagaa
    13801 aatgaaaata agccagaagt accgcctaca gaaaatccag acggagaaca gaaaccggaa
    13861 atagaacctg gagaagaacc cgatacagaa actcaaccag agccagacaa tgaatcaaaa
    13921 cctgaaataa cccctggaga aaaaccagat gtagacccag aagaaaaacc ggatgtgact
    13981 ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat
    14041 aatgaaactg aaaatccaga aaaaccagag gtagccccag aagaaaaacc agatgtaact
    14101 ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat
    14161 aatgaaactg aaaatccaga aaaactagag gtagatccag aagaaaaacc agatgtgact
    14221 ccagaaccag atacggacgc aagagaccaa ggaataccag aaaaaataaa caaaaaaaca
    14281 attcaagagg atggaaaaaa agagagtaaa aaatcaaatc tagcaattct aaaaataaat
    14341 gaagagcagc tgaataaaaa atcacgaata tttgatagcg cccaatcagc tgaaacctta
    14401 aaaagtagca aggatacaac ctttgctagt cctgaaacga aaaataaaca gctaccaaaa
    14461 tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca
    14521 acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc
    14581 tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa cgaccccaaa ataccagatt
    14641 gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt ttaagggata
    14701 tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag
    14761 gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt
    14821 aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc
    14881 agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct
    14941 cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct
    15001 gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag
    15061 ccaaatacag ccaaggcgat tgtagtgagc aacgtgttta aaagagcgat aacaaattga
    15121 gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga
    15181 aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt
    15241 tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct
    15301 atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg
    15361 tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc
    15421 tcgtaaaaat caataacgga ctggatcata tcaaatgttt gatgaataag catcgggata
    15481 tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa
    15541 acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa
    15601 gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa
    15661 acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa
    15721 acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg
    15781 aatatgaaaa taagaaagag aaagagcaaa aagaaaaacc gctaaatgac gttatcttat
    15841 gagttggttt tcctcataaa atcgttcatc tagcagtttc agcccttttt ctggtcttga
    15901 tgaaaattat ctagcatttt tcgggtcaga tgttgcagtg tatccaattc ttcctgttca
    15961 agtggaagat aagacttgat cttttgtgga ataccttctg cttgtttttg caacgcagca
    16021 ccttgaggag tcagcgtaac atttacgatt ctttcatcac ccgtttcacg ctgacgatta
    16081 atataatttt tttctgctaa cttttttagt aagggagtca gcgtaccaga atccagacac
    16141 aaatcatttc ctagctcttt cactgaagtc tttttctttt cccataaaac taacatcaca
    16201 agatactggg tataggtaag attcagcggc ttgaggaggg aagtgtagtg tctaatgatt
    16261 tccttagagg cggcatataa aggaaaacat acttgattaa ctaaatatag atcttccatt
    16321 tttccacctc cgctgtttta ctatagtgta tcatagcgga tttacacaaa aaagtctatt
    16381 cactaactgc tttcagagta acttctatat ttccagctgt agcttttgaa tacggacaca
    16441 ctttatgcgc tgcctctaat aattcttgcg tttcttccag agaaagacct tcgatagatc
    16501 cttcaatctc tacacctaag acaaagctcg tttcactttc tgaataaagg gaaacttgtg
    16561 cactgacagt agaagcccca gagatatttt tttgctgttt gatcaattct aatgcactgt
    16621 taaaacatgc actgaaacca gctgcaaaca attgttcagg gttggttgca ttttcgacgc
    16681 gtttaccagg agctgcaata ttcaattcga atgtacgatc gggattatga acttctccag
    16741 agcgtccgcc tgtgttgatc attttcgtag aatacatttt tttcataaaa ttaccacctt
    16801 taattatatt ttatacaatt gaattttaca caatataatt gtggaaagca aagaaatgga
    16861 ttggcataag ataaataaga gaaagcacat gaaacagacg cgctttctcg aatagcaaac
    16921 atatacaaaa aaatatcaga gacaaaaact aatctcatca taaacattac atatatttct
    16981 caaaaagttg ttttctttct tcaaaactta cttcacgatg gacctgatga agcatccata
    17041 aataaccaaa aggatctctg aacattgagt tagtcacacc aaagtccatc atttctgtca
    17101 tttcttgaat ctctacacag ccatttcgta atgctttatt atgtgtgtcc tcgatatcag
    17161 agacgatgat attgaaccag atcgtatttg gatgttcagc agtaggagca gtcaaaccaa
    17221 gagacgtgtt ttcatccagc atatggaatc tcgtgccata caaggtaaaa atgacttcat
    17281 tattgcctct agggtaatct gttgcctcaa tcagttgtat ttcatcaaag attgtttgat
    17341 acagttctag tgctttcaaa ctatcattta atacaaaatc tatttccgtt cctttatgca
    17401 taaagatctc ctctcctttt cagtcgaaca gttactagat tcttttatta taccagttaa
    17461 agaaaacgca ttcaaaaaaa tagcggattt acttaccaac tcattcgatt ggatgataag
    17521 taaatccgcg tcccattact tctgctacgc tgctgacaat gacaaaggca cttggatcga
    17581 tttcttctat caatggtttc attcgggaaa attcatgatc agtcacaacg atcaataaaa
    17641 cctcttttga ctttcgatta tacccgcctt ttgcctctag cagggtaagg ccacgtccaa
    17701 tctcagcact taagcgaagg cggatgtctt ccaaataatg ttcactcatg accataacag
    17761 atttctttcg attgatacct gtttcgatat aagtcatgac aattgaagtg attgcaatag
    17821 ataaaatagc ataaagaaac tcttctattc caaaaacaaa cagattaaga gaaacaacga
    17881 atgcgtctgt aaaaaataag ccaatcgaag tattcaagtg gaaatatttt ttaaaaatga
    17941 ggggagggat cgtcgtaccg ccgctagaag agttgttttt atataaaatc gcaactccta
    18001 aagcaaaaat cgcacttcca aaaatgacag acaacaaccg atctgaagta atcattgttt
    18061 ctggaactat tgcaatgatt aaaggaaaaa acaagcttcc gaaaagtact ttaaaaaagg
    18121 gttttttacc taaaaataga agagctagta ccaacataac gatattcaac actaaaataa
    18181 ctatagctcg gtcaaatcct aaaacagctt ccagtaatat tcctagaccg ctgacacctc
    18241 cagcagcaat atgatgaggc cctaaaaaca tattgatagc gactcctaat atgaagaggg
    18301 caaaaataat cttaatgtat gtccatataa attgtctcgt ttctt
//
LOCUS       DAJSMI010000045        18098 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJSMI010000045 DAJSMI010000000
VERSION     DAJSMI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.045"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            59..2119
                     /locus_tag="ON430_001220"
     CDS             59..2119
                     /locus_tag="ON430_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCR3219146.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2202..2771
                     /locus_tag="ON430_001221"
     CDS             2202..2771
                     /locus_tag="ON430_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3219147.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2946..3752)
                     /locus_tag="ON430_001222"
     CDS             complement(2946..3752)
                     /locus_tag="ON430_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219148.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3890..5404
                     /locus_tag="ON430_001223"
     CDS             3890..5404
                     /locus_tag="ON430_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCR3219149.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5807..6790)
                     /locus_tag="ON430_001224"
     CDS             complement(5807..6790)
                     /locus_tag="ON430_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCR3219150.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6795..7442)
                     /locus_tag="ON430_001225"
     CDS             complement(6795..7442)
                     /locus_tag="ON430_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3219151.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7527..8159)
                     /locus_tag="ON430_001226"
     CDS             complement(7527..8159)
                     /locus_tag="ON430_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3219152.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8175..8726)
                     /gene="coaC"
                     /locus_tag="ON430_001227"
     CDS             complement(8175..8726)
                     /gene="coaC"
                     /locus_tag="ON430_001227"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCR3219153.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8723..9460)
                     /locus_tag="ON430_001228"
     CDS             complement(8723..9460)
                     /locus_tag="ON430_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCR3219154.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9457..10446)
                     /locus_tag="ON430_001229"
     CDS             complement(9457..10446)
                     /locus_tag="ON430_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR3219155.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10704..11246
                     /locus_tag="ON430_001230"
     CDS             10704..11246
                     /locus_tag="ON430_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3219156.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11263..12348
                     /locus_tag="ON430_001231"
     CDS             11263..12348
                     /locus_tag="ON430_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3219157.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12360..13166
                     /locus_tag="ON430_001232"
     CDS             12360..13166
                     /locus_tag="ON430_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3219158.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13167..13994
                     /locus_tag="ON430_001233"
     CDS             13167..13994
                     /locus_tag="ON430_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3219159.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            13991..15064
                     /locus_tag="ON430_001234"
     CDS             13991..15064
                     /locus_tag="ON430_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3219160.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15492..>18098
                     /locus_tag="ON430_001235"
     CDS             15492..>18098
                     /locus_tag="ON430_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3219161.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIF"
BASE COUNT         5834 a         3211 c         3709 g         5344 t
ORIGIN      
        1 tcatcttatg aaaattacta cttcatgttt agacaagagt agggaggact aagaataaat
       61 gtgttcaaaa agacgttcaa aacaactaaa taaacacgct tgtcgtttag ataaggaacg
      121 gtcgtgttct gaaaaacact acgttaagtt tatgatgcgc acgatgctga tcagtttatc
      181 tgtaggtgtc tatatgcatg tacccgttca ggcaacgaca caaaccatca aggtcgtacg
      241 tacctctgtt tctgagacca ctgattcccc tatcactatt actcatactt tcactgatgc
      301 aaatggaaaa cagctacttc ctgcattgac ttcctataat aaagtcagtt atcctccaac
      361 gataaaccac tactggttaa ttcctaacaa aacattatgg acatcggaaa ccggaagcag
      421 tgtgccgctt tctatctttt ccgatatgac tgatcctaca aaaggtgatc agttaaaaca
      481 gatcactgat attttgaatc atgctttgac agatataaag gacggtggaa gcgcacaagt
      541 ttcttatgta tatgaaaaag attattcaca gtttgaaaga aagaaaatcg atgttctggc
      601 agggacaaaa gtttcgatat ctgatctggt agaaaggctg aagaacgcag atggaacaca
      661 aggagatatg tccaaagtaa tttcaaacaa tgggaatatc gtggatacga ctaatacagg
      721 aacatctact atttccttgt cctattttga tccaattgct ttaaaaacaa tgactgcatc
      781 tgctgtgatc catgttcgtg ctgatcaaac agctatcaaa ggaaaagatc ttcacttaag
      841 aacaggagag agttacgccc ctaaagatct tgttgaatat gtaacaaaaa gcgatggaac
      901 ttcaggaaat aaagaagaaa tcaaaatgaa cgggacagta gatactacaa ctccgggaga
      961 atatcccgtt gtattaactt atatagactc gaaaacatca aatgtggcag aaacaacagc
     1021 tgtagtttat gtagaagcat ctgctgcttc atcgactgtc atcactcatt ttatagatag
     1081 tcaaacaaat aaagaacttc aagcagaaac acatatgagt agtgtaaatc gtattcctga
     1141 tttgcgccta cctgctgaat tttctgatcg agtgccaaat atagatttat cgacttatga
     1201 acaaaatggg aaagtcactt ctatttctga ttggatgcaa gcaaacggat tagacagaaa
     1261 aagtcactgg tcggagatcc ttgaagtact tcactcagaa ctgatgaata ataggaagca
     1321 atcaggaggg gaaattacct ttacttatgt ttatatacca gacaatcgtt cattagaagg
     1381 tcatgatctt attgttcatc caaacgaaaa aataaacaaa gaagagttga tccaatcagc
     1441 aacaaattcc ttaggtaaac ccgtaccgtt aaaggatgta tcggttacaa tcaatgaagt
     1501 accattggag gatggttatt tgaccaaaca aacaggtgat tttacgatta tgtatagtta
     1561 tcatgacccg tattcactcc aagaactcca tgctcagtca ctattacatg ttttaccatt
     1621 aaacaaacag atagatcaaa aagaacaaaa aagacaagaa gaaagcttgt cagaaaaaaa
     1681 acaacaaatg gctcatgcag tttctccaac agggaaagta aaacaaacaa gaagcctaga
     1741 gttagcaaga atatctagaa aaatctcaga atctaataaa acgataaggc aagaagctca
     1801 tttggcccca caagtttctg gattggaaaa tgaaatttta gagcctaaaa taaaacaaca
     1861 atcagaaaaa gaatttgttg ctaaacagtc ggaagatcag aaggaatcag atgaccagaa
     1921 ggaaacgaag aaaaaacgct tgtcaactat tgattccacc gatgagcatc tgcagccgca
     1981 agtaccactt cctagtccgg gaacacccgc acctggtgga ggtattcctt cttctgcttt
     2041 aggagatttc atgcgaggta tggcaggaac atgggtatac gctaatcgaa atgaatttga
     2101 cgcagatgac tggttctgat ttatatctct atctgttata gtcaaataag tagctgagtc
     2161 atatgttatt tggcataatg gagacgagga gggatagaaa aatgttagat aaaacgttac
     2221 cttacgcaga aatctggatg acaagaccac ttcaacaaaa tgttccaatc acgcctttag
     2281 cggaaggttt tgaatttcga ttttatcaaa aagaagatga atctgcttgg gcggagattg
     2341 agacttccgt tggggaattt caaacgacag atgaagcact tgagtatttt caacaaaact
     2401 ttgctcctta tcctgaagaa cttgaaagaa ggatgttttt tgtagagaca aaaaaaggag
     2461 aaaaagtagc aacctgtacc gcttggcgaa aaaaatcaag agagcaagtg tatccggttt
     2521 ttcattggtt agcagtgaaa ccagattacc aaggaaaagg gctcgcaaaa gcattgactg
     2581 ccagagtctt gcaagaattt cctaccctcc atacggacgg accaatctat cttcacacac
     2641 agacatggag ccatcaggcg attgctcttt ataaaaaaat gggttttact tttattgctg
     2701 aaaatctaga cggcacacct aatccagatt atgaaaaagt tatgcaggtt ttaagtaaat
     2761 tagataaata acgaaatggt gaaataagtc ctcagtattg ggataaaaaa cgaaatagtc
     2821 attcctcata tcttactttt ttgagttaat actatcggat aactaatggc atgccataca
     2881 ttcaaaaaag aaggggcagt gacaaaagta ttgtcactgc cccttctctt gttttttagc
     2941 atgttttatc gtttgatttt tttaggaata aaattcttga tgaacgtaaa tttttggtaa
     3001 tcgtttcgac tatagaggta accaaataca ctgaaaacaa ctgctccaat aaaattaaca
     3061 aataaatcct tcatagtatc catgattccc acgtctaagt aaccattttg gatgattgtt
     3121 ttatctgttt taccatctgc attttgactt tcaatgattg tccgagtgat atctggaaca
     3181 tgatagacag tgtttccgtc tttacttaac tccacactat tgatttgctg gatgatcgta
     3241 tctttttgca tatcgaattg cagtaattta tctgctgaaa attccacaaa ctcccacata
     3301 acaccaactg tcatcgaaaa acagaaggaa acaactgcta gtaataaagg agtcaagttc
     3361 atggtttttg tatttttatt gagcaaataa acaagggaaa agccgacacc cccagctaaa
     3421 aatccgctga atgcatgtag aaaagtatcc cacattgaaa aatagccata aaaatcactt
     3481 aactctccta aaactgttga actaaaaata aaagataaca aaatgatttc tagcatattt
     3541 ggaatctgta tctttaatat cttttccaga aaataaggga tttcaaataa aattaatgtc
     3601 agtaataaaa gcattgtatt tccccagtgt tggttccaga cctgatccac tagcataata
     3661 acgacgatga tacgtaataa aatatagatt ccgtttgtga ttctacctgc agtttttgta
     3721 ctgttcgtaa aaaaggtttt caaatgattc ataaaataac tccttcatgt atatccttaa
     3781 ttatactaag aagcgtgtgt ttaccaaagc tatatgactt aaagaaaaac agagttgata
     3841 ttgtgaaaaa aatcacttaa tatagtataa ggagaaaagg gggatacaaa tgaaaaaatt
     3901 agttgctggt ttattaacag ccgttgcact tcttgctatg agcgcatgcg gcgcagatca
     3961 gaaaaataca gcgcagacaa gttctagtac gaaggaatct gccgaagtat catcaggcgc
     4021 ttctgaacaa gtatatacag atccaaaaga aatgaaagaa tcttatgatg tcgtgattat
     4081 tggagctggt ggtgcaggga tggcagcagc tatcgaagca aaagatgctg ggttgaatcc
     4141 agtaattttg gaaaagatgc ctgttgccgg aggaaacaca tctaaatcat caagcggtat
     4201 gaatgcttca gaaacaaaat ttcaaaaaga agaaggaatc aaagactcga atgatgcctt
     4261 ctatgaagaa acgctaaaag gtggtggagg aacgaatgat aaagaaatgc ttcgatactt
     4321 tgtcgatcac tctgctgatg cggtcgattg gctagatcaa aacggtatca aactggacaa
     4381 cttaacgatc actggaggga tgagtgaaaa gaggacacat cgtccgtctg atggctcggc
     4441 tattggtgga tacttagtgg aggggttatt gaataacgtc catgagcggg aaatccctat
     4501 ttttgtcaac gcagatgtga cagacattaa gaaaaatggg gaaacggtca atgaagtgac
     4561 tgtacatgtt caaggagaag aaccaaaaga ggtcaccgga aaagcaatcg tagttaccac
     4621 aggcggtttt ggtgccagca aagaatttat tgaaaaatat cgtcccgatt tagctgatta
     4681 tattacgaca aatcaagaag gatcaactgg agatggtatc accatgattg aaaaattagg
     4741 tggacaaacc gttgatatgg ataaaataca aattcatcca actgtgcagc aggatgaggg
     4801 ggtcttgatt ggtgaggttg ttcgcggaga aggagcaatc ttggtcgatc aagaggggaa
     4861 aagatttgtc aatgaaatgg atacaaggga taaagtatct gcagcaatta cagcattgcc
     4921 tgaaaaatca gcttatttga tttttgacca aggcgtgcgt gatcgtgcca cagctattga
     4981 attttatgac caaaaaggat atgtcacaac tggaaagaca atggaagaat tagcagaaaa
     5041 aatcgattta ccaaaagaaa cactagcaaa aacagttgat ggatggaata cggatgttgc
     5101 ggagaaaaaa gatgctcaat tcaaccgtgc cacagccatg gaacatgact tatcgtctcc
     5161 gaattattat gcgatcaaaa tcgcaccagg aatccattac acgatgggcg gtgtgaaaat
     5221 caatacgaac actgaagtat tagataaaga aaaccagcca attagggggt tatatgctgc
     5281 tggtgaagtc acaggtggct tacatggaaa caaccggatc ggtggaaatt cagttgcgga
     5341 gatcattatt tttgggcgac aagcgggcat acaagcagct cgatttgctt cggaaaatga
     5401 atgaaataga acgtaaataa aaagaggttg tgaaatcagc gttttgagct aataccgagg
     5461 gtcagctgat tgaacactgt ttagtgaaga ggttgtgaaa aagctgccct gcatcaagta
     5521 ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggttgga cttattaccg
     5581 cagatgcaag cttttgaaca ccgtttattc ggaaaaagag cgtgggacaa aagtaaagaa
     5641 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagaaa ttccttcgga
     5701 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt
     5761 ggagttaaac gcttctgtcc cagcctcttt tttgatcagc atgattttat tcagttatct
     5821 tcaaaatgat ttttccgatg tttttgtttg cttccatata ttcatgcgct tgtttgactg
     5881 cgctgaattg gaatgtacgg tcaatgattg gccgaatctt gccattttct agaaaaggcc
     5941 cagctttttg cataaaagtt tgtgcgagct ctgctttata ctgatcactt ctaggtgtca
     6001 ataatgtacc aagcagttgg acacgtttcc caatcaatac accaaggtct atctcaggca
     6061 cgatggtccc acctagcata ccaatcaaga cccaacgtcc atcaacggct atagaagcta
     6121 aattcttttc ccagtaagaa gcgccgatga aatccaaaat aacatcgact ccccgacctt
     6181 cagtgatttt tcgtacctct tcttgaaagt cttgtttttt atagttgatc aattcatctg
     6241 caccaagttc tttacaaaag gcgagttttt cttctgatcc tgcagtaacg atgattttcg
     6301 cgtgagaaag ctgtctagct aattggatcg ctgctgttcc cacaccactt gctcctgcat
     6361 ggatcaagac agtttcgcct tcttgtaatt ttccaagcca gtaaagtgtt tgataagcag
     6421 tcaaaaaaac ttctgagatt ccagcagcag aaacatagtc tagcgtatca gggagaagga
     6481 ttgcacgatc ggcaggcatc acagcatatt cggcataacc acctagattg actaatccgt
     6541 atacttttgt cccaggagta aaagtggaga tatcactttt attctcaact acctctccag
     6601 ctacttcgac tcccaaaata gggtagggag gtttcaaaga gactgattct cttttcataa
     6661 tatctgttcg attgacagct gctgtatgga ctttgatcag taattcgcct tgttttggtt
     6721 taggaacagg cacttcaatt tgttctaata catctacaga accaggatgc ttgattgaaa
     6781 tggcacgcat ggatctattc ctcctttgcc cattcttgga atgctgtatt caactctttg
     6841 ctaccttcag aaagtactag agtcgtacga gaaatatccg tttcttccac tagagcggca
     6901 agtacagcag caactgtaga gcgagtgatc gtttttgatg ccttctcatt aggcttagtt
     6961 aattgaatgg aagtaacttc ttcatcgtta gtcaatgtga ctggttgaag aatgacaaaa
     7021 tctaagttag ttcgtttcag ccattcatct gcataatgtt tagtaatata ataatctgtc
     7081 atggactcag gccattttgc tggttcatct gcataaacag cactgaccat cagatagcga
     7141 gagacatgcg cattttcagc tgcgatcact gttttgattg cgccatccaa gtctacttgc
     7201 agaagattct ttccttgaga gccggcagca aaaatcaata catcgatgcc tttgaatgct
     7261 tcggtcattt tttccacgga ccaagttaaa tcaaaagaga catacgaaac attctctttt
     7321 tttactaatg tttgggaagc aacatctctc acccctgcaa aaatttcatg ggaacttgga
     7381 gctaaatctt ttatcaggtg tcggccaatt tgtccgtttg cacctacaac aaaaattttc
     7441 atttggatca tcctttcgac gtcaaatctt tccattcaat agtaacacca aaagagaaaa
     7501 aaatcagaga tcggctacga tgactatcag ttagtaagag aagggtgctt gaagtatctt
     7561 aacaagacat tgcatactgc tgcagtcgca agacctgcga cgattccttc aggtatacca
     7621 cttgtaaaaa taaccgctag aatagctgaa taaacagcat ttaaatttgt tccgatgact
     7681 ttggcataat cttgctggaa taaaaagtag atcatattca tcactaaaat cgtattggtc
     7741 aatgaaccgg aaagtccagc taagaataaa gaaacggaat ctgctttttg tttcattagt
     7801 ttgcgcagac ctttataaac gaaataagga accaccccaa tcaagatgcg aggaataaac
     7861 gcgatcagca aggctttcca gcttccatgt tctgttccga tgactgggat aaacggagaa
     7921 aagacaaaag acaaaggtgt ttggataact gtgctacgga tcatgctgat cagtccaaat
     7981 gtaccgccaa gaaaagcacc tagtcttggt cctaaaacga tcgaagcaat gattacagga
     8041 atatgcatgg ttgttgcatt gattggaccg atagggataa aacctaaagg cgtaacagct
     8101 aataaaatca aaatagctaa aaacatcgca gttaatgtaa agttttttgt atttttcata
     8161 agtactcctt ttttctaagc atttctttca ttaagagtca gcatgacctg ttcgatgatc
     8221 gtctgaatat cagctaaggc accttttcca aaatctccac aagctaaaag agattctctt
     8281 ggttcaattt cctgatagcc gacagatctc aatgtttcca gattacgctg agtgatcggg
     8341 ttttgataca tattcgtgtt catggcaggg gcaatcaatt ttggtgttcc cactggaagc
     8401 gccattgcga ctgttgaaag aagatcgtct gcaatgccgt ttgctagctt gccgataatg
     8461 tttgctgtag caggcgcgat gagaaacaag tcagcttgtt ttgctagttc gatatgattg
     8521 attacctgcg gatttgattc ttgcatgacg tctgtatgga ctgcgcgctt cgacagagat
     8581 tgcaaggtta atggcgtgat aaaatgtgtg ctgcttttag acataatcac atctacctgg
     8641 gcacctaatt tgacgaattg acttgtaaga tcagcgcttt tgtaagcaga aatacttcca
     8701 gtaacgccta gtaagatctt tttcattttg attcctccag tctaattttc tcagtaatca
     8761 atgcagcaat ctcagattta ctttgagctt cttccactgt tccatcttta gataagagat
     8821 agccgtggtg tgtctcgcca tggatttccg tcaaatcatt tgcgaaaata aaatcagttt
     8881 tgttatttat cagactattt tttgcgactg ccagaagaga ttcctttggt acatctacta
     8941 atagtttgaa accaatgatt tttgcttttg gctgccattc acgtagataa gaaatgattt
     9001 ttggtgtttt cttcaaaacg agcacaagat gctcggtgtt agaagagatc tttgaaacag
     9061 tggtatctgt ctgttcattt tccgcaaacc aattgtccag ttcttcttgc gttgaagaag
     9121 tgggtaggtt ttttgccagc tgttcttctg aaaaactaaa agcaggagta aaatcactga
     9181 ctgccatact gtggataatc gcatcgtagt gttctttttt cgttagttct tctaattgta
     9241 aaaataagtc cagtgcagat tcgatcgtat aaatcgttat attggagtgt cttttcggtt
     9301 tcagtgcttg tttcgtagtc acgtaatcga ttgtggtatc cggattggca gctaaataat
     9361 cagctaatgc ttgccctaat cgtcctgtcg aatgatttgt aatagcacgt acttgatcga
     9421 ttttttcagt cgttcctcca gctgtgatta gaatcttcat ttatggtcac ctacatttac
     9481 tagtgttctt ccttggtgtg ttccagcaag tagtttagat gctgtttcag gcaattgatc
     9541 cagagtaatc tcttggacga ccagttcatc tgcaatctgc aaatccgcca tctgttgcca
     9601 taaagaaaga cgttgatcta ttggtacatt gacagaatca attccaatca actggatgtt
     9661 tcgcagaata aaaggcaaga cagtcgcgtt tagtttgatt cccccagcat tgccacataa
     9721 agctactgca ccgttataag agatcaatgg aagaagactg cttagctgct cgccacctac
     9781 tgtgtcgatg acatagtcga tttgctgttt tcctaaagct tttgttgtct ctggcagaaa
     9841 ttcatcaggt cgtacgactt gcaaagcacc tagacttttt agccatgtga cagactcttc
     9901 ttttctggaa aaggcaatga ttgactggaa tcctagttta tgtaacaggg ctatcgctgt
     9961 actgcctaca ccgccagaag caccagtaac gacaactttt ttatcggagg tcataccttg
    10021 acggattaaa gcgttcacac acaacatagc agtaaaccct gctgtaccaa ggatcatcgc
    10081 ttgacgcagc gtcatatttt ttggtagagg aactaaccag tctcctggta ctttttgata
    10141 ttggctataa ccgcctggat gggtgacgcc aagcccaaaa ccagtagcaa gtacaagatc
    10201 ttcttttgaa aaacgtggat tgttactttg gacgacagtt ccggataaat caatccctgg
    10261 agtcatcgga tatgttcgta tcacctgacc agactcgctc attgccaatg catctttata
    10321 attgacatca gaataggcaa tttttaccaa gacttcattt tcagccaact cagtaaatgg
    10381 cagatcactg attgctgtat ggaaaacagg ttcctttatt acctgaaaag atcgaaaagc
    10441 gttcattcgt gagacactcc ttttaaaatt ttcttttatc ataccataaa gcctactctt
    10501 ttattcttgt tcttccgtaa atttagacaa aaattaggtg aaaaataaag aatcaaacaa
    10561 ctagaattta ttccaaaaga taaagaaata ttcagcaaaa aaaattttac aaagggttgc
    10621 ctttttcaat aaatttcgta tactaatacg agtggtagta aacaggaagt ttactattag
    10681 aaaacttcga ttcggaggaa attatggaaa ttggtgagaa gttaagaaat ttgcgcatcc
    10741 aaaagaattt gacacaagaa gaactaggcg aacgcacaga tttgtcaaaa gggtatattt
    10801 cccagttgga acgagattta agttcaccat caatggaaac attcttttct attcttgaag
    10861 tcttaggtgt gactccagaa gaattttttc atcaagaagc agcgaatctg caagtggttt
    10921 attcaaaaga agatcatacg gtttatttcg atgaagaaaa cgggtatgaa ttggaatggc
    10981 tcgtggctga ttcgaatgaa aaagaaatgg agcctgtctt gctgactttt gaaggtgcag
    11041 gagaatataa gacgtttgaa ccttcattat ctgaaacgtt tatttttgta ttagaagggg
    11101 aattagagtt aagcttagga gagacggtct atgtggcaaa agcaggacag tcgatctatt
    11161 atcaagcgac aaaacaacat caattaagga atcatctcaa aaaacgaaca aaagtattga
    11221 ttgttgcaac gcaatcatat ttgtaatata gggggtaaaa acgtggggaa accaatcatt
    11281 tcatttgatc aagtagtgaa acgctatgat gatgaaacag tattaaaaaa agtaagtttt
    11341 gagatcgaac aaggaaaatt ttatacactg cttggtcctt ccggctgtgg taaaacaacg
    11401 atcctacgta ttatagctgg attcaccgaa gctacagaag gggacatcta ttttgaaggg
    11461 aaacggttga atgatttgcc agctaataag cgacaagtaa atacagtctt ccaagattat
    11521 gcactttttc ctcatatgaa tgtttttgat aacgttgctt ttggattgaa gatcaagaaa
    11581 atgtccaaat cagatatcga aaagaaggta aaagatgctt tgcgtatggt ccagcttcct
    11641 ggatacgaaa aacgggagat tagtgaaatg tctggtggtc aaagacagcg tgtagcgatt
    11701 gcacgagcaa ttgtaaatga accgaaagta ttgctattag atgaaccgtt atcggcgctt
    11761 gatttgaaac tacgtacaga tatgcagtat gaactgcggg atctgcaaca acgattagga
    11821 atcacgttta tttttgttac tcatgaccaa gaagaagcgt tggcaatgag tgatgagatt
    11881 tttgtcatga ataaagggaa aatcgtccaa agtggtacac ccgtagatat ttatgatgag
    11941 ccaatcaatc atttcgttgc tgattttgtt ggggagagca atatcgtgga cggtgtaatg
    12001 atcgaagata atctagttga gtttgttggt aagcaatttg aatgtgtcga tggcgggatg
    12061 cgtctaaatg aaccagttga agttgtttta cgcccagagg atttgacgat cacgacacca
    12121 gataaaggaa aactagtcgt aacggtcgat actcagctgt ttcgtggtgt ccattatgaa
    12181 atcatctgtt atgatgaaca gcaaaatgaa tggatggtcc attcaacgaa aaaagctaaa
    12241 gaaggaagca aagtaggatt ggcctttgag ccagaagata tccatgtcat gcgtttcaac
    12301 gagtctgaag aagaattcga tgcacgtctt gacagctacg aagaataggg gggacaagaa
    12361 tgaaaacaat gcgcaggatc tattccgtcc cttatatttt gtggctcggt ttattcgtga
    12421 tcgcgcctgt attaatgatc atttatcaat ctttttttga tatgaatggg caattcacgc
    12481 tgaataatta tctatcttat tttacgtcgg ggacatcgct ttctatgacg atcaattctg
    12541 tatggtatgc atttttgatc acgtttttca cgctgatcat cagttacccg actgcttatt
    12601 ttttaacaaa actgaagcac aagcaattat ggctgatgct gattattttg cctacatggg
    12661 tgaatctgct gcttaaagct tacgcattta ttgggatttt cagtgtgcac ggcagcgtga
    12721 atcatttctt tgattttatc gggatcgggc cgaaacagat attattcaca gacttcagtt
    12781 ttctgttcgt ggcaacgtat atcgagattc catttatgat tatgccaatt ttcaatgcat
    12841 tggaagagat gaatccttct ttgatcagtg ctagtagaga tttaggcgcg aatagctttg
    12901 agaccttccg acgagtgatt tttccgttat cattaaatgg tgtgaaaagt ggtgtccaag
    12961 cagtatttat tccttctctt tctttattca tgctgactcg tctgattggc ggaaatcggg
    13021 tgatcacttt aggaacggct atcgaacagc atttcttggt tacacaaaac tgggggatgg
    13081 gttctacaat cggtgtgatt ttaatcattg ctatgttcat cgtgatgctc ctgacaggcg
    13141 agaagaaaaa aggaggtcgc tcataaatga aaaaatttaa atggtcgtat ctttatttag
    13201 ggatcgtctt tttattctta tatgcaccga tcttctatct gattttttat tccttcaatg
    13261 aaggaggaac gatgaacagc tttacaggat ttacttggga aaattatgaa gctgtttttg
    13321 aagatacacg tttgatcatt atcgtattga atacgttcat gctggctttc ttatctgcat
    13381 tgatcgccac gatcatcggg acatttgggg ctatgggaat ctattacacg aaaaaacgac
    13441 gtgcacggac agcgttgttg agcatgaata acatcttatt agtctctcct gatgttatta
    13501 tcggtgctag tttcttgatt ttctttacac tactaggatt cggattaggt tttcagagtg
    13561 tattgctttc ccatattgct ttcagcattc caatcgtcgt cttgatggtc ttgccaaaat
    13621 tgcaggaaat gaacgattca atggtcgatg cggctagaga ccttggtgct aataatttcc
    13681 aagtagtcaa aaatatcatt ttgccttatc tttctccagg aatcatagct ggatatttta
    13741 tggcatttac ctattctctt gatgattttg cggttacatt tttcgtcaca ggaaatggct
    13801 ttacgacgct ctcagtagag atctattctc gtgcaagaca aggaatcagt ttagaaatca
    13861 atgcgctaag tgcgttagtt ttcttgttct ccatgatatt ggtcattgga tactacttta
    13921 ttagtaaaga aaaccgtcca aaacggctga agaaaaacag aagagtacgc gagggggtgg
    13981 cacggctgcg atgaaaaaat tacaatctct gtttttaggt attctcatca ttattgcggt
    14041 gctgctgctt ggtgttcgtc aacttgaaaa aacaagtggc atgagtggag caaaggtact
    14101 taccatctat aactggggag attatatcga tcctagtttg atcacgaaat ttgaaaaaga
    14161 atatggatac aaagtgaatt atgaaacatt tgattctaat gaagcgatgt tcacaaaaat
    14221 ccagcaaggt gggacaaact acgacatcgc tataccaagt gaatatatga tccaaaaaat
    14281 gatcaaagaa aaactagtac ttccattaga tcattcgaag atcaaaggat taaataatat
    14341 tgatccacga tttttagact tggattttga tccagataat cagtattcga ttccatattt
    14401 ttggggaact ttagggatcg tatataacga taaattcttt gatgcaaatc aaattaagca
    14461 ttgggatgat ttgtggaagc cggaattaaa agacagtttg atgctgattg acggtgcacg
    14521 agaagttatg gggctttctt tgaatagttt aggctattct ttgaatagta agaatatggc
    14581 acagctgaca gaagcagcaa ataaattgaa tcatttaaca ccaaatgtca aagcaatcgt
    14641 ggcagatgag atcaaaatgt atatgatcaa tgaagaagcc tccgtggcag tgacattttc
    14701 tggcgaagct gccgatatga tgtgggaaaa tgaacattta cattatgtga tcccaccaga
    14761 aggatccaat ctttggtttg ataatatcgt tatgccaaaa accgtgaaaa ataaagaagg
    14821 tgcctatgat ttcatcaact ttatgctaga accggaaaat gcggcacaaa atgcagagta
    14881 tattggttat tctacaccaa atgaagaagc aaaagcgtta ttgccaaaag acatttcgtc
    14941 ggacgaacaa ttctatccat ctgatgacac gatctcccat cttgaagtct acaaagatct
    15001 aggatcgaaa tacctaggca tctataatga cctattctta gaattcaaga tgtataggag
    15061 ataatggcaa aactcagtaa gaatggggac ttatcaacgt tctaaagagc taaaaatcgt
    15121 cctttttcta tctgagtttt ccaatcttct accaaaagcg gatgaaacgc cgcttttttt
    15181 gtgttatttt attttttcta ccacgttttt ctaccagaga aaagtggaca aaaccattgt
    15241 aaaatagggc ccgagacggg ctagtaaatc taagattgaa aaagttacag aaggataaag
    15301 taattggaaa ctatgcaaat atacttaacc cttctgtaat ctattaataa aactaatata
    15361 tgaatccagt tcaatatttg tagctatgaa aaaacataaa aatcatatta gataaaaact
    15421 aaattggtag ataaattatt ttgtcagata tctgaggcaa atattcgact atttgtgttt
    15481 caaagtaaaa attggaatac aaatatagaa tagacatatt gcagccgata gtttattatg
    15541 cttttagaca cattaaactg gaggaaaaaa tgaaaaagag cgctaaagat cgagtttttg
    15601 atgagctgat acgactaaca gggaatatgt cttctgtcac tgccgagaaa atttcgaaga
    15661 atctaggtat ctctcgtcag aatgccagcc attatttgac gagattagtg gaagataaaa
    15721 aggtcgagaa attaccggga aagcctgttt tgtggaagcc tttagatgaa tatgcggttg
    15781 tagataatac ggagcaaata aacgaagcct ttcattctgt agttggtcat gacggaagtc
    15841 tgagagaggt cattcaaaaa tgtatagccg ctgtcaaata tccgcctaac ggactcagtg
    15901 tattaataaa tggagccacg ggtgttggaa aaagtttttt agccacaaaa atttttgaat
    15961 acgctatcca tgagcaaatt attgaaaaag atgcaccatt tgctatctta aactgtgccg
    16021 attatgcgga caatcccgaa ctgctttcgg caacactgtt tggatacaag aagggggcct
    16081 ttacgggggc tgaaaaggat accgatggtt tattggcaac ggccaacaac ggctatcttt
    16141 ttctcgatga ggtccatcgc ctatcgaaag aaaatcaaga aaagctcttt ctatttattg
    16201 atactggaaa ctatcgtcca gtcggtgaaa atgtaaattg gcattctgct aaggttcgtt
    16261 ttattttcgc cacgacggaa aaaggtgaaa attatttatt agacaccttt gatcggcgta
    16321 ttcaaattag tgttatgttg ccaacttttg aagatcgccc cattcgagaa cgattagagc
    16381 tgattcaatt attttttcaa aatgaagcgg atgttttaca aaaagatatt attgtttcaa
    16441 gagaagcctt gtctttaatg ttatctcatc cctttagtgg gaatattggt aagctgaaga
    16501 atatcattaa aatcagttgt gcggatgtgt atagtcgaac acaagaagaa ccattaacga
    16561 ttgggaaaaa tgagatcttg attcagttaa atatgctgga atccgaagta gaaagcttgc
    16621 cgattgctag tttattgata aagaaaaatc aaaagataaa aacggatcga atcaaaccgt
    16681 ttgattatga ttcgattttg gaaaaaatta ctcagctgat aaatgaaggt aatttcaatg
    16741 aaaatttttc ttcgataaag aatgaagtac agcatttggt gcatcacatt gataaggatt
    16801 cttttttatc aatgagtaga aaattgatga ttcagatgtt tagaaaggtc tgggtatccg
    16861 ttttaggaaa aacgtacgga ttgacgacga gtggacctgt tgcagaaatt attagcagat
    16921 cctatgtcat ttccccaagc tataagacag acttaaccgg gataaatcgt acattagctg
    16981 caaatctacc tcggacgact tacttatgta atcagttctt ggagcattta ccaccgctct
    17041 cagaaaagga cgagaacttt ctaagactgc ttattgtcgt gactttatct gattatgtag
    17101 atgaatccat cgagctgaag ggcctgttgc ttgctcacgg agatagtacg gctagcagca
    17161 ttcaagcagt agtgaatcaa ttatgcggca attatgtttt tgaagcgttg gatatgccag
    17221 tagagagtaa tgtgtcagag attgtcgaat cgacaaggaa tttcgtgaaa cggcaagtga
    17281 atacagatag tctagtgctg atcgtagata tgggctcttt aaatcaaatt tacacacaga
    17341 tcaagaacga tttaaaaggg gagttacttc ttctgaataa tttgacgact tccattgcac
    17401 ttgatattgg cttgaagatg acgatgaatg cgccatttaa acagattgcg gaggatgcag
    17461 cagacaagta ttcaatcaat gttcaatact ttgaaggctt ttctcaaagt agcaacatta
    17521 tcatttcctg tatgtcgggt ttggggattt cggacaaatt gaaagatgtt tttcgtcatc
    17581 atttatcgga tgaatccatc gaagttttca cgaaggacta cagagaacta cgtgaattaa
    17641 tcgaccaaaa tgatgaaact tattttgaca agacaaaatt ggtcatcacg acttcggatc
    17701 ttccacgttc cttttcaatt cctaatatta atgtttatga catcttagac ggtgatggag
    17761 cagccaattt ggagcatatt ttgagtcctg aaatgaagcc ggcagaattt aaccgcttga
    17821 ttcaggagct agtaaaattc ttttcgttgg aaggaattgc ggaccgtttg aactttctga
    17881 acccaacgat cgtgatgagt gaagtggaaa cagtcatttc taaatatgaa aattactatc
    17941 atttcacttt aaatggccgt gtaaaactaa atctctatat gcatacggca ttgatgatgg
    18001 aacgtttatt cttggctcgg acagaaaaat ctgaagaacc gaattatgat ttgaaaccag
    18061 atgaagatga attttattat gtttctagaa gtattttt
//
LOCUS       DAJSMI010000046        17254 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJSMI010000046 DAJSMI010000000
VERSION     DAJSMI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.046"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(23..634)
                     /gene="rpsD"
                     /locus_tag="ON430_001236"
     CDS             complement(23..634)
                     /gene="rpsD"
                     /locus_tag="ON430_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR3219162.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            896..1747
                     /locus_tag="ON430_001237"
     CDS             896..1747
                     /locus_tag="ON430_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR3219163.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            1775..2410
                     /locus_tag="ON430_001238"
     CDS             1775..2410
                     /locus_tag="ON430_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCR3219164.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            2430..3203
                     /locus_tag="ON430_001239"
     CDS             2430..3203
                     /locus_tag="ON430_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3219165.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            3611..4408
                     /locus_tag="ON430_001240"
     CDS             3611..4408
                     /locus_tag="ON430_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCR3219166.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            4386..4799
                     /locus_tag="ON430_001241"
     CDS             4386..4799
                     /locus_tag="ON430_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3219167.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            4783..7428
                     /gene="mutS"
                     /locus_tag="ON430_001242"
     CDS             4783..7428
                     /gene="mutS"
                     /locus_tag="ON430_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR3219168.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            7446..9554
                     /gene="mutL"
                     /locus_tag="ON430_001243"
     CDS             7446..9554
                     /gene="mutL"
                     /locus_tag="ON430_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR3219169.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            9576..10136
                     /gene="maf"
                     /locus_tag="ON430_001244"
     CDS             9576..10136
                     /gene="maf"
                     /locus_tag="ON430_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCR3219170.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(10221..12209)
                     /gene="treP"
                     /locus_tag="ON430_001245"
     CDS             complement(10221..12209)
                     /gene="treP"
                     /locus_tag="ON430_001245"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCR3219171.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            12432..13145
                     /gene="treR"
                     /locus_tag="ON430_001246"
     CDS             12432..13145
                     /gene="treR"
                     /locus_tag="ON430_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCR3219172.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            13222..13668
                     /gene="msrB"
                     /locus_tag="ON430_001247"
     CDS             13222..13668
                     /gene="msrB"
                     /locus_tag="ON430_001247"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR3219173.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            13838..14440
                     /gene="ruvA"
                     /locus_tag="ON430_001248"
     CDS             13838..14440
                     /gene="ruvA"
                     /locus_tag="ON430_001248"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR3219174.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            14453..15454
                     /gene="ruvB"
                     /locus_tag="ON430_001249"
     CDS             14453..15454
                     /gene="ruvB"
                     /locus_tag="ON430_001249"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR3219175.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            15483..16061
                     /locus_tag="ON430_001250"
     CDS             15483..16061
                     /locus_tag="ON430_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3219176.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            16113..16595
                     /locus_tag="ON430_001251"
     CDS             16113..16595
                     /locus_tag="ON430_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCR3219177.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            16712..17086
                     /locus_tag="ON430_001252"
     CDS             16712..17086
                     /locus_tag="ON430_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219178.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         5726 a         3096 c         3773 g         4659 t
ORIGIN      
        1 tttttcaaga aagcttttga aattataagc tttggttgta gtattcaacg acaagagctt
       61 cgtcgatttc tggatatagt tcgtcacgtt ctggtaaacg agtcaatgaa ccttccaatt
      121 tttcagcgtc aaagcttacg aatgctgggc gtccaacagt tgcttctaca gcttctttta
      181 ctgttacgat gttttgtgat ttttcacgaa ctgagatcac ttgacccact tctacgtggt
      241 atgaagggat atcaacgcgt ttgccatcaa ctaaaatgtg accatggtta actaattgac
      301 gtgcttgacg acgagtcgtt gcaagtccta aacggtaaac aacattgtct aaacgttgtt
      361 ctagtaagat catgaagtta acaccatgtt taccttcttt gattttgcta gcttttacga
      421 acaagttacg gaattgacgt tcgttcattc cgtacatgtg acgaagtttt tgtttttcag
      481 ttaattgcat gccgtattct gattgtttgc cacggctatt aggtccatgt tgacctggtg
      541 cgtaagggcg acgtgctaat tcttttcctg tgcctgacaa tgagatgcct aagcgacgag
      601 aaattttcca tgatggtcct gtataacgag acattaaaat ttcctccaat gaatataagt
      661 ttttggagta aaataatccg ttgaaaaatt cataatcata tagtttgttc ttcaatcttc
      721 acctttgcag ccgcggttac acaatcgaac ccgagacggg gcaacaaact agggatgagt
      781 tattattttt ctgctgcatt attttacaca aattacagta tatgatagat catccacgaa
      841 tgtcaagata attagtacat aaaaacgctt tacaaaccta ggctgtcttt ataaaatgag
      901 agggtatgtg caaatggaaa aaagcgaagg aagtttaagg agaaaatcaa tgaatccggt
      961 atcaccatta tttgagaaaa tcgatgcttc aattgaaaag aaggcgaatc tttttgaaaa
     1021 cagcttttca cgttacgctg ttcgagcggt tctcgcctgt ttgtttttga ctttaggaac
     1081 ggctgtggca tttgcgatcg cgatgaaggg ggaagatatc gcgcatggtc taggaaagat
     1141 tttatacgca tttatgttca gctggggtct ggtcatgatt ctatacatga acgccgaatt
     1201 aggaacatcg aatatgcttt atatgaccgt gggtgttttt cggcagagac ttgctttaag
     1261 taaggcagcc aagattctat ttgcctgtat ttttttcaat atgattggcg gtatattatg
     1321 tggatttcta atttctatga ctacgccatt tcaagattta acgttggaca actttatgtt
     1381 agaatcaata gcagtaaaac tagcaaaacc ttcagcaacg attttggtgg aagggatgtt
     1441 tgcgaatatc gttgtcaata ccgctgtact gatcagcatg cggatgaaag aggatgtcgg
     1501 aaaagtgatc acagtcgtat tcatcatttt catttttgct tttttaggat atgaacacgt
     1561 gatagcgaat tttcctgcct ttacgcttac ttactttgct tctcacggac agatggatgg
     1621 aatgacagtc agcaatgtgt tacataattt gttttttgct ttggcaggaa attatattgg
     1681 tggcggttta gtaatgggac tgggatatgc ttggctgaat cagtcaaaat catcctatgt
     1741 ggactaaaaa gtcgttcatt aaaggggaag tgccatggaa gttttagttt ttccaaaaaa
     1801 tgaggaaaaa gcgttacaaa tggaaaagta catgaaaaat cttttttcct ttgctggagt
     1861 acctaagccg gaacgcgctc ttttacaaca accgttactg gatgagagca aaaatttatc
     1921 tatttctgaa gttttagctt ttgttcatta ttattatgaa caaatcgaaa gagagtatca
     1981 gtatgtagcg atcgatttag tccagaaaaa catcaaacgg cttacatttg aagacttagt
     2041 cgtattgttg ccactagttc agaaaaaaga atggtgggac agcatcgact cttggagaaa
     2101 agtatacagc acatggtgta aagcctaccc agagcagcgt gaagaagtct ttcaattgtt
     2161 tgaaaaaaag gaagattttt ggctacgtcg aatagcaatc acgttgcaat taggttataa
     2221 acagcaaaca gacacaggac tattgatccg agcaatagac gaagatcgtc aaacaaagga
     2281 attctttatc caaaaagcaa ttggttgggc attacgcgat tatagtaaga ccgatgccga
     2341 ttgggtaaaa agacagctga caacagattt gagtaagtta gctgttcgag aggcaagtaa
     2401 gtatttataa gaaagcgaga cgacaagaga tgagacgcaa attatttgct tttgatattg
     2461 atggaacgtt gttagattca aacaaacaag ctttagatag cacaagagaa gcattggaaa
     2521 aattacgcaa agccggtcat ctggtcacga ttgctacagg gcggagccga tttcatgcgc
     2581 aagagatcat tcgcgatctt gattttacga actacatttt atgtaatgga gcggccggtt
     2641 ttttagacca tgaacaagta tataagaatt tactagatcg cgaccagttg gatgcgttcg
     2701 ttcaagaagc gcatcatgag cagatcgata cagcttttgt cagcttagac aatatcaagc
     2761 gttcttcttc caatgatgta acggtgatgg aagaagcgat gcattctttc ggtgctgtct
     2821 tgcctgaatt agatcagttt tttgcagagg aacaagaaat ataccaagcg ttagcatttt
     2881 tcgatcaaac atatgatggg aagttctctt catatgataa aattcgattt gtccgctggc
     2941 atgagaacag tgtagatgtc gtacctaaaa atggttctaa agcagcgaca attctgcact
     3001 tagcagaaca agtaggcatc cctcatgaag acatcatcag ctttggcgat ggacaaaacg
     3061 accgtgaaat gttgcgcatg tcaggaattg ggatagctat ggggaatgct gtgccagaag
     3121 tacaagcaga agctaagatg gtcactgata caaatgatca ggatggaatt tggaaggcat
     3181 tgaaagagtt aagcgtgatt taaaagaggt tgtgaaaaag ctgttctgca tcaagtaata
     3241 agcccagcca aacaaaaata gctcctccta tttttcgttt ttctgggctt attaccgcag
     3301 atgcaagctt tcgaacaccg tttaaggaag aggtcgtgac agaagtggtc agtttcaaga
     3361 aataagaagg gatttacgaa aattgcttct caaatttttg taaaattacg gcttatttct
     3421 gaagaagtag atgaaacaac gtttattcgg gaaatgggac tgtgacaaga tgtttgtcat
     3481 agtcccattt ttttatttta tttttcaaaa ctaagaaata ccaggaaaga gttccttttg
     3541 tttgaccttt tctttcttat tgaaaacaac tataatatag agtgatgtta aaagagaaga
     3601 ggaatttagt gtgcgggtat tgtttattgg tgatgtagta ggttcaatgg gacgagagat
     3661 gattactgaa tatcttccta gattgaagaa gaagtaccgt ccgcaagtaa cgattgtaaa
     3721 tggtgagaat gccgcttctg gacgtggaat cacagaaaaa atctataaaa aatttttgca
     3781 agacggcgta gatgtcgtga cgatggggaa tcatacgtgg gacaaccgcg gaatctttga
     3841 gtttgtaaat gaagctaaaa agatggttcg tccggcaaat tttccggaag ggacgcctgg
     3901 acaagggatg gtctttgtca aagtgaacca agtcgaactg gcagtgatca acatgcaggc
     3961 tcgttctttt atggtggact tggatgatcc ttttcgtaaa atgaatgagc tggtagaaga
     4021 agccagaaaa cgtacgccga tcatttttgt agattttcat ggagaaacaa caagtgaaaa
     4081 acaagcaatg ggctggtttt tagatgggaa agtttcagca gtggtcggta cacatacaca
     4141 cgttcagaca aatgatgcgc gaattttgcc aagaggaaca gcgtatctga cagacgttgg
     4201 gatgacaggt ccctatgatg gtattttggg tatgcgcaga gaacctgtaa tcgaaaagtt
     4261 tctgactgca ttaccaaaac gttttgaagt tgtggaacaa ggccgatcga ttttaagcgg
     4321 ctgtatcttg gaattggatg atacgacagg tcatgcaaaa gaaatccagc tgattcagat
     4381 caacgatgat cgaccgttca tggagtagaa agagaggcag ttttttgcag caggagccgg
     4441 aaattcaaaa agaagtaagg aaactgacaa aacttttaag agagaatgaa acgatcattc
     4501 gctacaaaga acttgaagaa aagatccagc aaaaccagta tttagctgag ttgagagaaa
     4561 aaatcaaaca agcgcaaaaa gatgctgttc attttgccca ctatgacaaa ccagcagcag
     4621 aaaaagaagc aatcaaacaa gcggatcaat tcatgcaaga atttgatcag catccgcttg
     4681 ttgtagctta tagaaaacag ctgttggaag cagatgatct gcttcatcat ttaacaacta
     4741 tgatacaaga agaaatcaat ggacaaatag aggaggaaaa acatgcctca aaaaactaaa
     4801 aatactccta tgatggaaca atatctaagc atcaaggccc aataccaaga tgcttttttg
     4861 ttctatcgtt taggagactt ttatgaacta ttttacgaag atgcgatcaa agcgtcacag
     4921 cttttggaat taacgttgac gagcagaaat cgaaatgcag aagaacctat tcctatgtgc
     4981 ggtgtacctc accatgcagc acaaggatat atcgatgcct tgattgaaaa aggctataaa
     5041 gtagcgattt gcgagcaggt agaagatcca aagacgacta aagggatggt caagcgcgaa
     5101 gtgatccagc tagtcactcc tggtacagtc atggacagca aaggattgtc ggcaaaagac
     5161 aacaattttt taacagctgt agttagtcaa ggaaatgaat ttggctttgc ttatgccgat
     5221 ttaagtacgg gagaattgaa gacggctcgg ttgcatgatg aagaagctgt tttaaatgaa
     5281 gcctctgcat tgcagacaaa agaactcgta ttaggcagtg agatcactga tacccttcgt
     5341 gaacagctaa gcgggcgttt agggctagtc ttttctcaac aaaatgagat ggaagaaaac
     5401 gcggaattta gctttttgac aagcgaacta gtcgatccgc tggaaaaaga agcaaccggc
     5461 aaactattga cttatttatc ggtgacacaa aagcggagtt tagctcatat ccaaaaagca
     5521 gtagaatacc aaccagatca ttttttgaaa atggattatt attcaaaatt caacctagaa
     5581 ttaagccaat ctattcggac tgggaaaaag cacggcactc ttctatggtt gttagatgaa
     5641 acaaaaacag caatgggtgg ccgtttacta aaacaatggc tagaccgtcc gcttatccag
     5701 gcaaaacaaa ttaaagcaag acaagaaatg gtcgcttctt tgctagacag ctattttgaa
     5761 cgtatcgatt tgcagtcagc attaacgaaa gtctatgatc tggaacgcct agcaggtcga
     5821 gtagctttcg gtaatgtcaa tgggcgagac ttgatccagc tgaaaacttc tttagaacaa
     5881 gtgccaatga tcagagggtt gattgaagga atcgatcaag gtcaatggca aagtctgctt
     5941 tcagaaatgc agccgatgga ccatctcgtc caattgatcg accaagctat ccaagatgat
     6001 ccgccgttac aaataacgga aggaaacatc atcaaagatg gattcaatga gcagctcgat
     6061 atgtaccgtt ctgctatgag aaacggaaag aaatggttgg cagaacttga agcaaaagag
     6121 cgtcaagaaa caggaatcaa gaacttaaaa atcggattta accgcgtttt cggttattat
     6181 atagaaatta caaaagcaaa tcttggaaat gcagaactgg agaagtatga acgaaaacaa
     6241 actttagcaa atgcagagcg tttcatcaca ccagaactaa aagaattgga gacacaaatc
     6301 ctcgaagcag aagaaaaatc tgttgattta gaatatcagt tgtttttagc tgtgagagaa
     6361 gaagtcaaaa aggctatcca gccgctacaa gtacttgcaa aagcaatcag tgcagtagat
     6421 gtgctacaaa gttttgcaac gatcagcgaa cgctatcagt atgttcggcc agaacttgtc
     6481 agtgacaagc atcagcttct gatcaaagac ggacgacatc ctgtagtaga aaaagtatta
     6541 ggacatcagg aatatatccc taacagcgta gaaatggcag aagacgaaat gatcttgctg
     6601 attacaggac cgaatatgtc tggtaaaagt acgtatatgc gacagttggc attaactgtt
     6661 ctgatggcgc aaatgggctg tttcgttccg gcaaaacaag cagttttacc tatttttgat
     6721 cggattttta cccgaattgg tgcaagtgac gacttaattg ctggacaaag tacttttatg
     6781 gtagagatga tggaagccaa tcaagccctg cgttatgcta cgccaaacag tttgattttg
     6841 tttgacgaac tagggcgagg aacagcaacc tatgacggaa tggcgttggc ccaagcaatc
     6901 atcgaatata tccatcgtca tgtacaagca aaaacactct tttctactca ttatcatgaa
     6961 ctgacggtac ttgagaaaga acttccgcag cttaaaaatg tccatgttgg agccgttgaa
     7021 aaagacggag aagttgtctt cttgcataaa atgatggatg gacctgcgga taaaagctac
     7081 ggcatacatg tggcaaagat cgcaggattg ccttccaatc tactagaacg agcagcagat
     7141 atcctgcata cgctagaaaa tggcgataac ggacaccata ctgttccagc tgaagtagaa
     7201 gagaaaacta aaacagccaa acaggagcaa ataaaaccac ctgcagaaaa agtaaatcaa
     7261 gaccatatac aagaccgtgt aaaagaagaa aaccaacagc tttctttatt cagtgaatta
     7321 tccgaaaatg aaacagaagt cattgaatca ctgaagcaaa taaatttact agaaatgacg
     7381 ccaatggatg cactaaacac attatatgaa ttacaaaaac aactgtagga agaaggcgga
     7441 tatcgatggc caaaatccaa gaattatctg aacgtttagc taatcagatc gctgcgggag
     7501 aagtagtgga gcgaccagct tccgttgtga aagaattagt ggaaaatgca attgacgccg
     7561 gcagtactca gattgatatc ttagttgaag aagcgggact gcggacgatc caagtgattg
     7621 ataacggaga aggcattttg gcagacgacg tagagaatgc cttcaagcgg catgcgacaa
     7681 gtaagatcca taatcgcgac gatctttttc gcatacgaac gctgggcttt cgaggggaag
     7741 ctttgccaag tattgcctct gtttctgaaa tgactctgga aacggcgaat caggaagaaa
     7801 aacaaggcac ctatctgtcg ctgaaaggcg gagaagttat tgagcatcga ccagcaccac
     7861 tgcgtcaagg aacgaagatt accgtcagca atctgttttt caatacgcca gcccgcttga
     7921 aatacgtgaa aacactgcac acagaattag cgaacatcgg ggacatcgtc aatcgactgg
     7981 cattgagtca tccatcgatt gcttttcgtt tagtacatga tggaaacaaa atgctggcaa
     8041 ctgctggaaa tggcgatctg aaacaaacaa ttgcgggaat ctacggatta tctaccgcaa
     8101 aaaaaatgct gaaaatcgaa ggaaaagatc tagattttga gttatttggc tatgtttcat
     8161 tgcctgaggt gactcgtgcc agtaggaatt acttatcgac gatcatcaat ggacgtttta
     8221 tcaagaactt ttccttgaat aaagcaatcg ttgcaggtta tgggtcaaaa ttaatggtcg
     8281 gtcgtttccc tattgctgta ttggaaatca aaatggaccc gcttttagta gatgtaaatg
     8341 ttcatccgac gaagcaggaa gtccggctgt cgaaggaaaa agaactaaca gagctaatta
     8401 gtaacgccat tcaaaaagca ttgtctcaag aaaatctgat tccaagtgca gcggataatc
     8461 tacggtttaa gaaaaaagta cctgatcagc ctaaaacaga acaaatcgaa atcgatttgt
     8521 cctacgcttt tgaagaagaa aaacctaaaa agccaagtag tttgaatttt gatcgagaga
     8581 caggtacctt tttcgtagaa gaactaaaga acacatttgt tgacagaatt gttgataagt
     8641 ctgtggaaaa tgttgaaaac tattctggtt tttctgaaga aaacaccact tatccagaaa
     8701 aaacagcgaa aacggtggat aagtttggtg aaaatgttga taactttgta gaaaaaacag
     8761 tggaaaaatc tgaaaagaaa gaatttccca ttgtggataa catggagaaa acaaatgaag
     8821 aacaagcaga taaatccgta gaagaggatg aaaagattta cgaggaagaa ttgacactcc
     8881 atcctgaatt tgatttcact caaggaaacg acaaaacatt agaacaagcc atcaagaaat
     8941 tagaaacgga aaaaccagta caacgttttc ctcagttgga atattttgga cagatgcacg
     9001 gaacgtatct ttttgcccaa agtagcgatg ggttgtacat cattgatcag catgctgccc
     9061 aagagcgcat taagtatgaa tatttccggg aaaaaattgg ggaagtatct gatgatctgc
     9121 aagaattgct tgttccattt gtgttagact atccaaacag tgatgctttg aaattaaaag
     9181 agcaaaaaca cttgctagaa gatgcaggga tttatttaga agaatttggt caaaacagct
     9241 ttattgtacg ggcccatcct acttggtatc ccaatggaga agaagaatcg attattcggg
     9301 agatgatcga tatgctgctg acaactggaa gtgtgagtgt aaaaaaattc cgtgaagcaa
     9361 cagcaatcat gatgagttgc aaacgatcga ttaaagccaa ccattatttg aatgaagcac
     9421 aagcacgtgt gctgctgaag gatctggcac gatgcgaaaa tcctttcaat tgtccccatg
     9481 gccgtccagt attgattcac tttaccaatt cggatatgga aaggatgttc aaacgaatcc
     9541 aagacccgca ttaagtcaaa gagaagaggg aaaacatgaa gattgtatta gcatcccaat
     9601 caccaagaag aaaagagtta ttggctcata tcgtttcaga attcgagatt gaacctgctg
     9661 atatcgatga aacgccattt ccagacgaac agcccgtcga ttatgttcgt agaatggctg
     9721 aagaaaaagc acgaactgtc tgggaacaag atgaaaaaga agaccattta gtgattgcca
     9781 gtgacacgac tgttgttctc aatcaagaga tcatgggtaa accagaagat ctggctgagg
     9841 ctgaattcat gctgaaaaaa ctaagcgggg aaacacatac tgtatataca gctgttgttt
     9901 taaaaacaaa ggaacgagaa gaacggatac tagcagaagc acatgtcact ttttatcctt
     9961 tgacagacga agagatcaaa cgctacttgg agacggggga ttatgcggat aaggcagggg
    10021 catacggcat ccaaaatgct gcgagtgtat tcgtcaaaga aatttctgga gattattaca
    10081 gtatcgtagg atttccgatt ggtgctgtca atcaggcact gaaaacattc atttaaacaa
    10141 aaaagcggac tatctgattt tcgattgaaa atccatgata gcccgttttt tttattgaaa
    10201 agattactta tctactctga ctaagatttt acttcagctt taccgactgc ttcgcccggg
    10261 cgaacaagtc ctgattggtc aagagaaagt cctgcaactt tttcattatt ggtcaaagca
    10321 acaataatgt cagttttctt gccagcttgt tctatttctg gcaaatccat ttcagcaatc
    10381 agggtctctg gtgtgacaag gtatccttct ttcacgaata cattgaaagg aacacccttt
    10441 agttcaacag tatctaatcc catgtgaaca agtacttcca gcccctcatt agaaagaata
    10501 ccaatggcat gttttgtttc aaagacactc gtcactttac cgtttactgg tgcatagact
    10561 ttgccgtttg acggttcgac agcataaccg tcacccatca ttttttgtga gaaaactggg
    10621 tcatttactt cagttatttc cttgatggtc cctgcagcga ctgcgaataa tgtttctttt
    10681 gttgcaactg ccgtaccagt tgctgaaaca ggttcgaaag actgtggcgt tgctgttgcg
    10741 ccatttgctg tttgaagttg tacatcagca gcattttcag gaacagcagg atcaatctta
    10801 ttcaaaattc cctgacgtct aaagatgatc gtcaagccaa atggaatgat gacagcaatg
    10861 atcattgcga tgatgaaagg tacccatgtt tctgcttgga tagctagtat tcctggcaaa
    10921 ccgcctactc cgatcgcgtt agctcgaaca cccatcaagt tagcaaacat gccagctaat
    10981 ccagagccga ccattgcagc gacgaaagga tagacgtatt tcaaattgat tccgaacata
    11041 gcaggttcag ttacgcctaa gtaacaagaa atcattgctg gaatcgagat ttgttcttct
    11101 tttttattcc cacgatgtaa gaagatgatg gctaagactg cagagccttg ggcgatattt
    11161 gataaagcaa tcatcggcca taaattcgtt gatccgaaat ctgcaatcag ctgcatatcg
    11221 atggcattgg tcatatggtg gagaccagta atcactaaag gagcatacag gaatccgaat
    11281 acggcactaa acaaccagct gatagaagac gtcaatccag tatttacgat atttgagatc
    11341 cagcttccga ctgtccagcc gataggacct aaaataacgt gtgcagctaa aacagttggt
    11401 aacaatgaaa acaatggaac aaaaatcatc gagatcgact gagggatata tttgcggaag
    11461 aagatttcta gatacgctaa cataaaccct gccagcatgg ctggaataac ttgcgcttga
    11521 taaccaatca tctggacttg agcaaaacca aaatcccaaa atggaatatc tgctgcggct
    11581 gtggaagcta cactataagc atttaataat tgcggagaaa ctaacgtaat tcctaaaacg
    11641 attcctaata tttgtgtagt acccatcttt tttgcaatac tccaagtgat acctactgga
    11701 agaaaatgga aaattgcttc acccggtaac cacaaaaagg cattgacacc attccaaaac
    11761 ttcgagtgtt caacgattgt tccaccaaga ctttcaaact ggataccttc aagtacattt
    11821 ctgaatccaa ggatcaatcc accgacaatg attgcaggaa tcagtggtgt aaagatttct
    11881 gctaaaacgg caatagcacg ttgaacggga tgaaggtttt gttttgcagc tgcttttcct
    11941 tgttctttgg aaaccccttc aactccagat actgcaacga agtcgttata aaaagtagaa
    12001 acatcgtttc ctatgatgac ttggaactgt cctgcattcg tgaacatccc ttttacgcta
    12061 ggtatatctt cgatcgcttt ttcgtccgct tttcctggat cattcaggac aaaacgcata
    12121 cgagtcgcac aatgactaac tgcagcaata ttttcttttc cgccgatatc atgtaacagc
    12181 ttctcggcat ctgcttgata ttttgccata tcaataaacc cctttctctt aacttgtcta
    12241 tgcaagttgt ttcttcattt actatacttt ttttcggaga gaattgcaag cgttttatta
    12301 aaaagtacga gagaaaatga tatcgtttta aaaagcttaa aataagcttt tcttaaaaat
    12361 aggaacattg aaatacaaaa agagctgtag tatagtgaag aaatgatgga agaacgagtg
    12421 agggaaaaaa catgaataag ttcaatgaaa tttttttaga tttggaacag aaaattttag
    12481 cgaaagaata tcctccgcat tcgcttttac ccagtgagaa ccaattgatc aaaatttatg
    12541 gtgtttcacg agaaacaatt agaaaagcgc tgaatctttt gactacggaa ggttatattc
    12601 aaaaaaaaca aggaaaaggt tcgatcgtac tggatcggaa tcgcttcgac tttcctattt
    12661 ctggattgac tagctataaa gagctacagg agacccaacg tattccaagt gaaacgatca
    12721 tccatacgtt agaagaaaca gaagtgacaa aagcattgaa cgagatcact ggctgggaga
    12781 tcggtgcgcc tgtttggcat ttgatacgcg aaagaaaaat caatggcgaa gtagttatat
    12841 tagacaccga ttatctatta aaggaaatcg ttcctatttt aactccagca caagcacaag
    12901 gatcgatcta tgaatatttt gaaaatgagc tttctttgac gattgcttac gcgcaaaagg
    12961 agatcacagt agaagaagta acggaaatca tgaaaacagc gatggatctt cacgatgaca
    13021 cttatgcagt agtagtcaga agcctcgttc atctggaaga tacccgctgt ttcgagtatt
    13081 ctgagtcggt tcaccgatta gataaattcc gtttcgttga gtttgcccga cgccgaaaag
    13141 tgtaaatagt tcaaaagaaa gcaaattgtt ggcatctgtc cactttggcg ctatgatagg
    13201 atcacaaagg gggcattttt aatggataaa ccaataagac cagaaaaaga agaactgaaa
    13261 gaaaagttat cacccatggc ttatgcagtc acacaagaaa atgcaacaga acgaccattt
    13321 agcgggaaat atgatgattt ttacgaaaag gggatctatg tagatatcgt cagcggtgaa
    13381 ccgttattca gctctgcaga aaaatatgat gcaggttgtg gctggccatc attcagcaaa
    13441 ccaataacga aaagacaagt gcgcgaaaaa gccgattttt ctcatggcat gcatcgaata
    13501 gaagttcgaa gcaaagaagc agattctcat ctcggacatg tgtttaccga tggaccagta
    13561 gatcaaggtg gtctacgtta ctgcatcaat tctgcctcac tgaaattcat tccatatgaa
    13621 aagatggatg aattaggtta tggggaattc aaatcacttg tagaataatg gaaatacaca
    13681 aagagcggta caatacgggg atttactcga tcgtccgctc tttttttatt caagcgattg
    13741 ctatttgaaa gaaacgctcc ctagactcct tttttcacaa ggtgaaatgt gctacaataa
    13801 agggaacgta tgtgtgcaaa agaaagagga ctagaatatg tatgaatatc tgacaggtgt
    13861 ggttaccttt atcaacccct attacatagt agtagaaacc aatggtatcg gctaccagat
    13921 cgcactagga aatccttacc gctacagtag caaactaaat caaaaaataa aaatttatgt
    13981 ccatcaggta atacgtgaag atgcgcatct tctgtatgga tttgattcgc tagaagaaaa
    14041 gcaactgttt ttgcgattag taagtgtttc aggaatcggt ccgaaaagtg cccttgcaat
    14101 catggcaagc gaagaccact ccggtttgat tcaagcagta gaatccagtg atgtcactta
    14161 tttgacgaaa tttccaggag tagggaaaaa gacagcacaa caaatgatct tggatctaaa
    14221 aggaaaattc ggtgagctga gtatcgatac accttttagt ctatttgatg aagcaaacac
    14281 aaaagacgct acagctctat ctgaagcaat ggaagctctc tcggctcttg gttacagtga
    14341 tcgagaaatc aaacgtgtag aaaaacaatt aaaagaagta gataatcaga caacggatga
    14401 gtatctacgc caagcactca agctaatgat gaaaaaataa ggaggagacc aaatgacaga
    14461 tgaatacttg ttatcaccag aatctggcga aaatgaactg agtttggaaa aatctcttcg
    14521 accacagctg ctttctcaat atatcggaca gagaaaagtc aaacaagaac ttcagattta
    14581 catcgaagct gccagaaatc gggaagaacc tttagatcat gttctgttgt acgggcctcc
    14641 tggattagga aaaacgacga tggccatggt cattgctaat gagatgaccg taaatattcg
    14701 tacgacgagt ggccctgcta tcgaacgtcc gggtgactta gtagcaatct tgaatgaact
    14761 ggaagcaggg gatgtcctgt ttatcgatga gatccatcga ttacctagag tggtagaaga
    14821 gatgctttat tcagctatgg aagatttcta tgtagatatc atggtaggac aagggccaac
    14881 cgcacatccg gttcatttcc ctcttcctcc ctttactttg attggcgcga cgacacgggc
    14941 aggtatgctt tctgcacctt tacgagatcg atttggcatc atttctcata tggaatatta
    15001 tgacgaacag gatctgcagg agatcgtttt acgttcttcg gatatttttc aaacagagat
    15061 cattgaagaa ggggcattgg aaattgctcg ccgatcacga ggaacgccta gaatcgctaa
    15121 tcgcttgctc aaacgggttc gtgattttgc gcaagtccaa ggagacggga aaatcgatcg
    15181 tcctatagcg gatcaggcac ttactttgct acaggtcgat caggctggat tggattatgt
    15241 tgaccagaaa ttgttgcgaa caatgatcga gttatatggc ggcggaccag tagggttaag
    15301 tacgctttca gtaaacatag gagaagagcg tgagacggta gaagatatgt atgaaccgta
    15361 tctgatccaa aaaggatttt taaagcgtac acctagaggg cgaattgcta ctgcttatgc
    15421 ctatgaacat tttggttacg actatcatat atgatttttg cttttagatt ggaggaaaat
    15481 gaatgagtga tacaacacgt acgaaacaat tgatcaaaaa cgtattgatt gatttgtgcc
    15541 aaacgcaatc ttttcgaaaa atcagcgtcc aaaaaatcgc aaacgaagca aaaatcaatc
    15601 gacaaacatt ttattatcat tttacagata aatacgattt gcttcgttgg gtctattgtg
    15661 aggatgcatt gcattattta gatccacaat gtctttcact tgataattgg gaagagcaag
    15721 cattgaaaat gctgaaagca attgctgaga agaaagaatt ttatgcaaat actgtgcttt
    15781 ctgacccaga aattttacaa aaagagtttt ctcaactgat ccaaaaatca tttatcaaga
    15841 tttttgaaca aatggatgaa gaaagacaac tttctgcaag agacaaacta ttttatgctc
    15901 gttttttttc ttacggttgc agtggtgtat tagtcaattg gatcacgaat gaatatgaag
    15961 aaacaccgat ggaaatcgct atgcagcttt tccgtttagc caaggatacg gaattatatt
    16021 cttatcgtct ttatgcaaaa gaagagttgg aagaacagta atagaaaaat tttttgttgg
    16081 aaaagcaata atcgctaaaa ggagatgggg aaatgacaaa aattttattt gtttgtttgg
    16141 gaaatatttg ccgttcaccg atggcagaag gcttgctgag aaaacgaata gcattagaag
    16201 gaagggaagc agaatttttt gtggattctg ctgctacaag cacgtatgaa gttggaaaga
    16261 caccgcaccc tggaacgaaa aaaatattaa accaagaaaa agtagatatg acgaatatgg
    16321 ttgctcggca aatcacaccc cacgattttg aaacttttga ttggatcatt ggcatggatc
    16381 aagaaaatgt agaagaactg aaacgaagag caccacgctc agcgcagggg aaaatccatt
    16441 tgtttttaag tagcattcct ggaaaagaaa aagaaaatgt tcccgaccca tactatacca
    16501 ataactttga tgagacatat cacttgatca cagaaggcct gaatcattgg tggaatattt
    16561 ggattgatga aaataaaata acaaacaatt tgtaaagtta acgctttctt tttaaggcga
    16621 tgttagagca tttccttgtt tttttgtaaa atgttaggta aaataaaatt aattaagtaa
    16681 cactttctta gctaaaggag gagtgcattc gatgaacgaa gagacagtat cgccaatgaa
    16741 gaagcttacg aagttagtta ctcatacttc gtatcaactt tttgattctt ttgtaaatga
    16801 cactgaagaa catgctaaga atgtacgatt gcaaggaaag tttattcttg agctgcaaaa
    16861 agctgctact gaacatagtc tagttatctt acaagtaaga gcaaatcgtt tgagtagcaa
    16921 gtatgaaacg atctcaggct gggtggcgac aaaaacggtc gaaaaagacc gagtgatgat
    16981 ccgcctacaa aacgaagagc agcagatccg gatggttcct gtagaacaaa ttgaaaaaat
    17041 aacaatcctt tcaccagcag gggaacagga acggttatca aaataattta taccagatag
    17101 aaaagcacgt atgagcttgt accatcaatc attactaatc aaaaataacc gaatgcttta
    17161 agctgatatc agtaaaacgg aacgttttag gatgagtatc atagcatgac agcattcggt
    17221 tgattagttg aatcgattgg atggtacaag tttt
//
LOCUS       DAJSMI010000047        17248 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJSMI010000047 DAJSMI010000000
VERSION     DAJSMI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.047"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            1..369
                     /locus_tag="ON430_001253"
     CDS             1..369
                     /locus_tag="ON430_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR3219179.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ON430_001254"
     CDS             549..1490
                     /locus_tag="ON430_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR3219180.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ON430_001255"
     CDS             1559..2290
                     /locus_tag="ON430_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR3219181.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ON430_001256"
     CDS             2434..4614
                     /locus_tag="ON430_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR3219182.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ON430_001257"
     CDS             4634..5116
                     /locus_tag="ON430_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCR3219183.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ON430_001258"
     CDS             5132..5893
                     /locus_tag="ON430_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR3219184.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="ON430_001259"
     CDS             6024..7358
                     /locus_tag="ON430_001259"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR3219185.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7786..8271
                     /locus_tag="ON430_001260"
     CDS             7786..8271
                     /locus_tag="ON430_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCR3219186.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8264..8947
                     /locus_tag="ON430_001261"
     CDS             8264..8947
                     /locus_tag="ON430_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR3219187.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9073..11718
                     /gene="polA"
                     /locus_tag="ON430_001262"
     CDS             9073..11718
                     /gene="polA"
                     /locus_tag="ON430_001262"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR3219188.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11763..12599
                     /gene="mutM"
                     /locus_tag="ON430_001263"
     CDS             11763..12599
                     /gene="mutM"
                     /locus_tag="ON430_001263"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCR3219189.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12563..13192
                     /locus_tag="ON430_001264"
     CDS             12563..13192
                     /locus_tag="ON430_001264"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR3219190.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13244..13576
                     /locus_tag="ON430_001265"
     CDS             13244..13576
                     /locus_tag="ON430_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR3219191.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13888..14379
                     /gene="nrdR"
                     /locus_tag="ON430_001266"
     CDS             13888..14379
                     /gene="nrdR"
                     /locus_tag="ON430_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCR3219192.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14402..15775
                     /locus_tag="ON430_001267"
     CDS             14402..15775
                     /locus_tag="ON430_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR3219193.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15775..16701
                     /gene="dnaI"
                     /locus_tag="ON430_001268"
     CDS             15775..16701
                     /gene="dnaI"
                     /locus_tag="ON430_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCR3219194.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16830..>17248
                     /locus_tag="ON430_001269"
     CDS             16830..>17248
                     /locus_tag="ON430_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219195.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSI"
BASE COUNT         5868 a         3027 c         3805 g         4548 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttcccgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt tttcttggct gttcttatta cttgatgcag ggcagctttt tcacgacctc
     7561 agataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa tgaaaaatat
     7621 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     7681 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7741 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7801 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     7861 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7921 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7981 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     8041 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     8101 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     8161 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     8221 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     8281 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     8341 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     8401 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     8461 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8521 ctactctctt ttgaatggca taacattagc ggttactctt gcatactacg atattggaac
     8581 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8641 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8701 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     8761 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     8821 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8881 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8941 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     9001 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     9061 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9121 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     9181 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     9241 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     9301 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     9361 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     9421 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9481 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9541 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9601 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9661 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9721 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9781 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9841 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9901 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9961 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
    10021 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
    10081 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10141 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    10201 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    10261 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    10321 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    10381 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    10441 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagataa aaaccaagaa
    10501 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10561 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10621 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10681 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10741 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10801 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10861 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10921 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10981 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    11041 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    11101 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    11161 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    11221 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    11281 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    11341 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    11401 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    11461 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11521 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11581 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11641 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11701 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11761 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11821 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11881 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11941 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    12001 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    12061 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    12121 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    12181 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    12241 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat
    12301 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    12361 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    12421 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12481 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12541 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12601 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12661 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12721 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12781 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12841 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12901 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12961 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    13021 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    13081 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    13141 aaaacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    13201 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    13261 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    13321 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    13381 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    13441 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13501 tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13561 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13621 ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc
    13681 tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat
    13741 tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg
    13801 tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat
    13861 aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca
    13921 cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt
    13981 gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta
    14041 atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc
    14101 cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc
    14161 gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat
    14221 gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat
    14281 cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca
    14341 aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc
    14401 cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc
    14461 tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg
    14521 agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg
    14581 tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta
    14641 tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag
    14701 tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt
    14761 caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa
    14821 gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa
    14881 aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat
    14941 cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc
    15001 gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga
    15061 tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac
    15121 acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga
    15181 actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt
    15241 tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct
    15301 tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc
    15361 agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca
    15421 atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa
    15481 taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa
    15541 aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca
    15601 atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa
    15661 gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc
    15721 agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa
    15781 gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct
    15841 gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa
    15901 gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa
    15961 gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag
    16021 ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca
    16081 cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt
    16141 caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa
    16201 gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat
    16261 ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta
    16321 gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa
    16381 caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt
    16441 ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc
    16501 ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt
    16561 acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta
    16621 aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt
    16681 cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa
    16741 aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt
    16801 aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag
    16861 ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg
    16921 attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca
    16981 tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa acgcttcagt
    17041 tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca
    17101 atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga
    17161 aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc
    17221 caccgcaagt tcctggagat tcgattat
//
LOCUS       DAJSMI010000048        16867 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJSMI010000048 DAJSMI010000000
VERSION     DAJSMI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.048"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(296..1024)
                     /locus_tag="ON430_001270"
     CDS             complement(296..1024)
                     /locus_tag="ON430_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR3219196.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ON430_001271"
     CDS             complement(1002..2576)
                     /locus_tag="ON430_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR3219197.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ON430_001272"
     CDS             2805..3743
                     /locus_tag="ON430_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3219198.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ON430_001273"
     CDS             3755..4666
                     /locus_tag="ON430_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3219199.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ON430_001274"
     CDS             4690..6162
                     /locus_tag="ON430_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3219200.1"
                     /translation="MKKNMQKYLIGGVSVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6214..6825
                     /locus_tag="ON430_001275"
     CDS             6214..6825
                     /locus_tag="ON430_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3219201.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6855..8768
                     /locus_tag="ON430_001276"
     CDS             6855..8768
                     /locus_tag="ON430_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HCR3219202.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8896..11877
                     /locus_tag="ON430_001277"
     CDS             8896..11877
                     /locus_tag="ON430_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HCR3219203.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11874..13535
                     /locus_tag="ON430_001278"
     CDS             11874..13535
                     /locus_tag="ON430_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HCR3219204.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13575..14507
                     /locus_tag="ON430_001279"
     CDS             13575..14507
                     /locus_tag="ON430_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCR3219205.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14867..16432)
                     /gene="guaA"
                     /locus_tag="ON430_001280"
     CDS             complement(14867..16432)
                     /gene="guaA"
                     /locus_tag="ON430_001280"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR3219206.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(16487..>16867)
                     /locus_tag="ON430_001281"
     CDS             complement(16487..>16867)
                     /locus_tag="ON430_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3219207.1"
                     /translation="YHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGT
                     EGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRI
                     AFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         5373 a         2774 c         3399 g         5321 t
ORIGIN      
        1 atttaaataa ctacatccat ctattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt acaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgtttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgacga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttta ctaaatgaaa
     6181 agaatagact aagcagttag tggggggatt caaatgaacg aaaacaaatg gaccaaagga
     6241 tttaattggg tttttcaatt gttattacta aactttactt attttatact aatttgttta
     6301 ggatttggaa ttttaggttt tttccctgct acgatagctt tgattgctgg cgttgtgaat
     6361 cgaaaaaaat atggcttgca agagtctgtg aagttcatga gtaaaatcta taagaaaaat
     6421 tttgttagat ataatctata ttgctggtca gcaactttgt tgttggccgg caatattact
     6481 acgatctggc tttgttggca gcaaagcgca ccattgtttt atttattaag tacattatta
     6541 tcggtaatta cactgttcct agtctttgca atgttgaatt gggtcgtgat caaaagtctt
     6601 gggttgtgga tgatgggaga ccgactgatt gactatttgc ttttcacagc aacgatgttg
     6661 ccaacggtag tcatgcaagg cttggtgctt ggaatttatt tccgaatatt gatgattttg
     6721 cctgtgattt ttctgtttgg cggaacagct gtaacggtaa tattgattag taaattaact
     6781 gaaagagcgt tagcaaagtt gccttgtcag atacaatcgc actaattttt tcacaaaaag
     6841 gagtatttct ttctatgaag ttaactgata aacaagtagc taatatagcc tttatccagc
     6901 aacttgagat tccattgaca caaaaagatg aagaacaaat agacttgatt tttactgaca
     6961 caaaggctaa tgtgcgacaa atcaagatta ctaaaaatga gcagcaacga atctgcttgt
     7021 cttattcaca tgaagacttg ctgtatcgag gattgtttat ctgccttcca cgttttttag
     7081 ctcaaggagg agagatgcca tacattgaag aaacctcgat cattgagacc agttattcat
     7141 tagatgcttc tcgtaatggc gtgatgaata aagagactct aaaaaaattc atttcttact
     7201 tagcgttgat ggggtacaac acattgtata tttacacaga agatacctat gaagtagaag
     7261 accttccata ttttggttac cttcgcggag cgtatacaaa agaagaagtg aatgaaattg
     7321 ttgaatttgc ttctggtttt ggcatagaag tagttccatc gatccagacc cttgctcact
     7381 tgacccaatt tttgaagtgg tatgctcaac aagaactgat ggatgatcaa aatactctac
     7441 tgatcggtga agaaaaggtt tatgcggcaa tcgaaaagat gattattgcc tgcaaagaaa
     7501 tgtatcagac agagcgtatt catttgggaa tggatgaagc ctatcaagca ggtctgggac
     7561 gatatttaag cctgtatggg tattgtgatc ggacaacttt aatgattcaa catatggacc
     7621 gtgtgttatc aattgttgat aaatataaga tgaaaccaat gatttggagt gatttcattt
     7681 ataagctatt agatgaaacc ggtaaagata ggtattacaa tcctgatttt aaactcaaag
     7741 cattagcagc taaaggatta ccagcaggat taacctatgt tcattgggac tatggttcag
     7801 atgaagtttc ctcctatgag aaagtaattg ataaccattt gaaattttgt gacattgatc
     7861 attacgtttt agctagtggt gcccatatct gggggagact agccccgaat catgggaaaa
     7921 cagaagcaat tgtttctgca gctgtaaaag cttctaaaaa taaaggtgta cgttctgtga
     7981 tgttaactac ttggggagat gaagggcagg aaacagagca ttggcattcc ttaattgggg
     8041 cattaaaatt agcagattat gtctattctg aagaagagtg gaatgatttt gaggaaatct
     8101 acgatcgttt attaggaaca ggtagttatc aattaatgtc cgaattacga tatttcgatg
     8161 aagtggatac cattacaaat aacaacttac aaatggcttc cgtgagtaaa ctgctacttt
     8221 ggcaagatcc gttagttggt atatatgact ttcatgtagc cgctcataac aaaattgcca
     8281 ctagaacaat tggtacttat tatcatgagt tagctcagaa attagatatg aataaaaatt
     8341 ggaatccaaa acaaccaatt ttaacaacta ttcgtcaacg atatatactt ttagctgaaa
     8401 ttttagcaca aaaggcagaa ctaggcatct atttacaaca ctgctgtctg gtagaagacc
     8461 ttgcaggatt aatggaatta gcgaatcggt gtgaagcact agccaatcga gtaaatgaat
     8521 tggcatctat tcatgaaata atttggtatc aaacatacaa aacctatggt tgggaagtat
     8581 tagaacaacg atatgctgca ttagctcatc gtctgaaaac cgttgcgcgt cgtattaaaa
     8641 atcatcttca ggggcaagat gccctagagg agcttttgga agaacgatta ccgttttgtc
     8701 aatatcaagt gccgcttgaa atttctggct ttaattatcg gcaaagctcg gtgtcaggct
     8761 acaattaacg aaggaatctt cccgttgaac ggaccacttg ctcatctaat gggaaagtgg
     8821 ttcgttttct attcataagt agcttaccac taactctttt attaagaaga gaaaaaaagt
     8881 aggaagtgag aaaatatgtt atcggactat tatcaaaatc tgtcagttat aagtgtcaat
     8941 acagaaccat tacgtaatta ttatattcct tatgcaagta aagaagaagc aaaacaagag
     9001 ttagaaaggg aaaaaacgag ccagtttatc gatctcaatg gtacatggga ttttcagtat
     9061 tatccttctg tccaacagtt accggaaaat ttttttcaag aggcctttac tgaaagttat
     9121 gatagaattt ctgttcctgg agtttggcaa acagagggat ttgacttacc tcagtatatc
     9181 aatgtatctt atccaattcc ttttgatccg ccttatgtac ctaatgatac cccttgtggt
     9241 gtatatcggc gtcagtttca aattgagcaa acagatgcac gcttttactt aaatctagaa
     9301 ggagcagata gtaattgttt tgtttggata aatagaaact ttgtaggcta tgaccaagtg
     9361 agtcatagta catctgaatt tgatgtgacc aagtatttac aggttggggt taatgaaatt
     9421 caaattctag tgatgaagtg gtgtgatggt acctactttg aagatcagga taagtggcga
     9481 atgagcggtc tatttcgtcc agtgtatctt cttcaacgac caactatgca tctgacaagt
     9541 tatcaactac ggacgttgac tgatcctaaa aaaggaagcg gaacattaat ggtttctttt
     9601 caacgattta ctcctattga agtatcgtta cctatttctt acgaattatt ccatcctgat
     9661 ggacgtctca tagcgaaagg ggagtgttac tcggaacaac aaataaattt ggatgttgaa
     9721 gattgccttt tttggacagc agaaacgcct aaattatata cattactttt aacatgtaat
     9781 ggtgaatatt ttaaagaaaa agttggcttt cgagcactga cgatcaagaa tcgtaagtta
     9841 ttgctgaacg gaacacctat taaattatat ggagtcaact atcatgacgt gaatcagcaa
     9901 ggcacgccag tgatgacccg agagcagtta cgtgcagatt tgttattaat gaaaaaatta
     9961 aatgtgaata ctgtacgaac ggcacattac cctaaatcac cagattttta tgaattggcg
    10021 gatgagattg gtctgtatgt aatcaatgaa gcagatttgg agtgtcatgg tgtggttaat
    10081 ctatatgata ctgaagcgaa ttatcaacta attgctaatg atccgacatt cgaaaagccg
    10141 attatggacc gagtgatccg ttctattctt ccttatcaaa atcgaagctc tatcattatg
    10201 tggtctatgg ggaatgaatc tggttatgga gtaaattttg aaaaagctca agctttagca
    10261 cggaaattgg atgattcacg gttgattcat aatgaacgct cgattgaacc agttattgac
    10321 caagtgaatg attttagtaa cttggatgtt attagtcaaa tgtatccccc actggaagat
    10381 gttcatcgtc attgtcaaag aagtgatact gaaaaacctt tttttatgtg tgaatatagc
    10441 cacgctatgg gcaacggtcc tggagatctt aaaacgtact tcgatttgat tcaacagtat
    10501 gattcctttc ttggcggttg tgtctgggag tggactgatc atgcaatcaa tagaggaact
    10561 ttagaaacgc catcttattt atacggtggt gattttggtg aaaaatatca cgacggcaat
    10621 ttttgcgtag atggtttatt gaccccagat cggcaattaa cagtaggtgc attggagtac
    10681 cgtaatattc attcgcctat tcaactgatt gctgtagatg aggagctgca agaagtaacc
    10741 ttgagcaata taactgcttt tacagccggt tcagcattta acctttctct tgaatggtat
    10801 atagatggaa aaaaaacagt ttatgaagta atgaatcctt tcatacttga gcctggcaaa
    10861 agttgttcat tgcccatcac agtacctaca acgattcatg gtgctcatac ggttgctgtt
    10921 tcgctatctg tttataaaaa tgatgagctt tatggacagc aacagctctt tcttaaaaaa
    10981 gaaaaaatga gattgccaag aactgctcat cataatgatg caccacaaat ttcagtaaaa
    11041 ggcacagagc tgataatcaa aggagaatcc tttatttata tatatcaatt agcaaaagct
    11101 gcgttcactt ctgtaaagat caatcaaaag gaacttttgg aacgtcctac tcaattttca
    11161 atttggcgtg caccaacgga taatgatcgt aacttagtaa acaaatggaa agcagctgga
    11221 tatgctgaaa tgtctttaga aatcagaaaa aaagactgga aattcgtaga tgatcatctc
    11281 ctgatcgaaa cgtcttatcg aggagttatt ccaggattac agccaatttt tacaatgcac
    11341 atacagttga aaataaatgg tgcaggcgta atcaaccaaa aagtatcagt ggagaaaaat
    11401 ccgatttttc cagatttccc tcgctttggg atggactggc cgttgaatga tactttttcg
    11461 aaggtttctt attgtggcta tgggccgtat gaaagttatc ctgataagca ctatgctgcg
    11521 atgtttggta tttatgagat ggagattgag gaatttgatc agccatatat caaaccacaa
    11581 gaatatggta accgctggca atgtgattgg gtatcaatca aaaatcatca aggatctacc
    11641 atgacggttt atggaaactc taatttttct gctctgcctt atagtcaagc tcaattaacc
    11701 gaaaagtcac accattatga attaataaaa gaaaatagaa cttggttcaa tttaagttat
    11761 aaacaaaatg gtatcggctc acacagttgc ggtccttatt tagaagatcg tttccgtttt
    11821 atggaagata cttttgactg gaatatagtg attactttca aaggagaaaa tagatgaaag
    11881 aaacaaagat caatcaagtc aaaaaaaaat tatctcgtta tcaagaagtt gtttatcgaa
    11941 accattgctt aaaatggtca aaaaataagc tgaatgttcg ttttaatgga ttattttatc
    12001 gtttcgaaca gttagaagaa gtctggaaac cggtaacgaa tttccaatca tgtcctgtac
    12061 agctcataaa tgatccaaaa gaaacatacg atttatgtat aacacacctt tataaccagc
    12121 atttgaaaaa tgacggattt tatttaaagg ttactaatga caatcaactt acgattgaat
    12181 cagcaaacct tcgagggttt caatatgcta tggaagcctt attagagagt tttttttcgg
    12241 taggagatca attattatta ccggaattga ttctgaaaca tgaaccatca gtgaaaattc
    12301 gaggaatcat tgaagggttt tatggtatcc cctggacaca tgaaatgcgc ctctctcttt
    12361 tttcatatat gaaagataac caattgaata cttttatgta tgcccctaaa gatgatgaat
    12421 tattacgtaa aaaatggcgt gaactctacg atgcgcaaga attagacaaa tttaaggagc
    12481 ttttatctgc tgctgaggct agcaatatcg atttttggta cttaattagt cctggaaatg
    12541 atattgatat tacgtgtgaa gaagacattc aagttcttct gaaaaagtta gaacaactga
    12601 ttgagcttgg cgtatttcaa tttggtctat taatggatga tattgactat caattaaaag
    12661 gtgctgctaa acgccgtttt cgagagccag cattcgctca tgcctattta gtgaatcgag
    12721 tagaggaata tcttagcact cggttgtcca atcatgaatt agtcatttgt cctacagaat
    12781 atgataatcg tcatggttct cgctatttag caacgttaag tcaagaaata ccagaacaat
    12841 taccgttctt ttggactggc ccaagcacgt tagcggcgtc aatttctaca gaagagctgc
    12901 aggaaatggc ttctgtctat caacggccga tgctgatttg ggataatatt ccggtaaatg
    12961 attatttaga ggataaagaa ctattgttca tgagtccata tgagaatcga acaccgaatc
    13021 tttctaagga gagataccag gttacaggag tagtttcgaa tccaatggct caattagaag
    13081 cttcaaagtt tacgatcaat agtatggcta attacttatg gaactgtgag cgctttgatc
    13141 ctttggaaac ttggacgtca gtcgttgaaa aagtagttgg aagtaaacta caaccagctt
    13201 atcttacctt aaccaatgct tttccaaatc attacacaag cagtctgctt tcagatgaag
    13261 aattactgct agccaaagac ccagaatggg tcacaaaaga aatggctgct ttagtccaag
    13321 cagtaaaatg gatccagcaa gaacaacata catctagatt gagaacagaa ttggagcctt
    13381 ggttacaagc aatcacagaa agttggactt tctggcaaga atggcaatta aaaaaagatc
    13441 caaaagaagc agaagaatgg ttggctaaga aaaaaacaca ccgcatagga cgtgatttag
    13501 taactgccca tttagagcaa attatcaaaa gttagatatc ttaaaaaata aaaagcaaga
    13561 aaaaggagaa caaaatggtc aagagattaa ttagtgcaaa ctacagtgat gtcaagaaaa
    13621 tgacgtcaaa agagttgaag caatccatca aagcgagtga aggacgggtg attgtttgtg
    13681 aaacggttgc taatactctt ccacaatctg gagaaattac gaatgcagaa gttgcgtcag
    13741 catttggtgc agatttgatt cttttaaatg gtttcgattg ttatcgtcct gcagtttttg
    13801 gactaccaaa tgtgacgatt gagaaggttt ttgatctaca gcaaacgatt gaaaacccga
    13861 ttcctaaatt aaaagaacta gtcggtcgtc ctgtgggaat taatttagaa cccattgccc
    13921 ctaaaacaaa tatgatgagt gagaaagtag aaatcagcca aggaagagca agctctgttg
    13981 aaacgataca aaaagcagaa gaactaggag tcgattttat ttgctttacc ggtaatccag
    14041 gcactggagt aaccaattta gaaattacta aatccgtaaa aatggcaaaa aagtattttt
    14101 cgggattgat tattgctggg aaaatgcata gcgccggctc agaagagcca gttgtgaata
    14161 aagaggcgat tatttcttat gcggaagcag gcgcagatat tttactagtt ccttcagtag
    14221 ggtctatccc aggcttttta gagcaagacc taattgaagc ggttcggttg gcaaaaaaat
    14281 atgatttgtt aacgatgtct gcaatcggta ctagtcagga aagtgcccga ccagaaacat
    14341 tgcggcaatt agccattatg aataaaagtt gtggtgttga tttacaacat attggagatg
    14401 ctggatacgg gggattggca ccagcagaaa atattttcga aacctcggtt gcaattagag
    14461 gaatgcgaca taccatcaac tgcgttagcc gctcgatcaa tcgctaacaa aattaaataa
    14521 aaaatggtta aattgattat ttaagtggga acatacttga gatatcagct aacttctgtc
    14581 attttaaaaa aagggactgt gacagaagtg aaaatagaca aaaaatatcc gaacaagaga
    14641 ctaggatagc agaaaaacta tcgttttgca acgaggacca actggaacgt aggagcaatt
    14701 ctcttattgt tcggatattt cgcttgttca aggacttatg tctgtagtgc ccttttttgg
    14761 taggaaaaat ggtagggaac catatcattt ttaatgaaaa tcaagcattt caaaagttga
    14821 aaaacgttga tttatcggca ttccccatcc atattatatt tacgtcttat tcccactcca
    14881 ctgttgcagg tggtttgctg gtaatatcat acacgatacg gttaacgtgt gcgacttcat
    14941 ttacgatacg gacactgatt ttttgcaaga cttcccaagg gatacgggca aagtcagcgg
    15001 tcatgccgtc aatagaagtg acagcacgga tacctacagt gtagtcatac gtacggccat
    15061 ctcccataac cccaacagag cgaatgcctg gtaggacggt gaagtattgc cagatgtcgc
    15121 gatctaaacc ggcattggcg atttcttcgc gcaagatagc gtctgattca cggacgattt
    15181 ctaatttttc ttctgtcaat tcaccaagga cacgaatacc taagcctggt ccagggaatg
    15241 gttggcgcca aacgatgctg tcaggcatgc ctaattgggt acctaattca cgtacttcgt
    15301 ctttgaacaa tgtgttcaat ggctcgatca gcttgaattg catgtcttca ggtaatccac
    15361 ctacattgtg atgtgatttg atggtttggg cagtttcagt tcctgattcg atcacatcgg
    15421 tataaagggt tccttgtgca aggaaagaaa cgccttcttc accggcaagt ttggttgctt
    15481 catcatcgaa gacatataca aattcatttc caatgatttt acgtttttct tctggatcag
    15541 acacgccagc aagttttgat aagaaacgtt ctttggcatc gacttttata atgttcaagc
    15601 caaattttcc gccaagactt tccataactt gttctgcttc acctttacgc aacaatacgt
    15661 gatcaacgaa gatacatgtt aattgatcgc cgatcgcttt ttgtaaaaga acaccaacaa
    15721 cagaggaatc tactccgcct gaaaggccta ataaaacttt tttgtcaccg acttgttcac
    15781 gaatttttgc gatctccatg tcgatgaagt tatccattgt ccagtcacca gtacattggc
    15841 agacatctag tgtaaaatgg cgcaacaagt catttccata ttcggaatgg cgaacttctg
    15901 catggaattg gattccgtac attttgcgtt ctgcattttg gatcgatgcg atggggcaat
    15961 ctttgctggt agcaacgaca tcgaaatcag tcggtgcttg ggtaactagg tcaccatgac
    16021 tcatccaaac ggtttgtttt tcaggagtgt tttggaaaag ggccgcttct gcagaagtga
    16081 tttccaattc tgcttttccg tattcacggt ttttggctgg tttaactttt ccgcctaatt
    16141 tataagtaat caactgcata ccgtaacaga ttcctaaaac tgggatcccc aaatcaaaaa
    16201 tcttttcatc aataccaaag gccttctcat cataaacact gtttggtccg ccagaaagga
    16261 tgattccttt agggttcatt gcctttactt cttctgcggt aatgcggtgg ctcatcaact
    16321 ctgagaaaac accgaactca cgaatacggc gagtgatcag ttggttatac tgactaccgt
    16381 aatcaagtac gataattttt ggctcttcgt caaatcgtgt tggtgaagac aaatttgaat
    16441 aaaacttagt ccgatatgcg gcgcgttctg tgcgccgcat atttttttag ttttcaaaga
    16501 tcagaccttg ttgtaaatag atccgtgccc gaaagttata aaagttgcga taaccaaacg
    16561 caattcgttt aatcagcttg atcttgttat tcagcccctc agtgacgccg tttgagtggg
    16621 acaccttaaa ggcatttgaa atacccgatt ggtagcgatg gaagatcgtg aatttctttt
    16681 taaacgactg aggtaacgta gatgaaattg ttttaagggt ttcttgaaag ccttccgtac
    16741 ctcgtttaac gaaataatag cgtaataatt gacaaaaatg atacgcagtt cgcaattctt
    16801 cgttgtagct taacagctca tcaacgattt ctgtttgact aattggtcgt ttaaacagtc
    16861 gatgata
//
LOCUS       DAJSMI010000049        16849 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJSMI010000049 DAJSMI010000000
VERSION     DAJSMI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.049"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(359..3064)
                     /locus_tag="ON430_001282"
     CDS             complement(359..3064)
                     /locus_tag="ON430_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCR3219208.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3370..4317)
                     /locus_tag="ON430_001283"
     CDS             complement(3370..4317)
                     /locus_tag="ON430_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR3219209.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADRADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4507..4776
                     /gene="rpsN"
                     /locus_tag="ON430_001284"
     CDS             4507..4776
                     /gene="rpsN"
                     /locus_tag="ON430_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR3219210.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(4952..5314)
                     /locus_tag="ON430_001285"
     CDS             complement(4952..5314)
                     /locus_tag="ON430_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3219211.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5325..6446)
                     /locus_tag="ON430_001286"
     CDS             complement(5325..6446)
                     /locus_tag="ON430_001286"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR3219212.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6462..6812)
                     /locus_tag="ON430_001287"
     CDS             complement(6462..6812)
                     /locus_tag="ON430_001287"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR3219213.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(6942..8453)
                     /locus_tag="ON430_001288"
     CDS             complement(6942..8453)
                     /locus_tag="ON430_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3219214.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8775..10127)
                     /locus_tag="ON430_001289"
     CDS             complement(8775..10127)
                     /locus_tag="ON430_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR3219215.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10280..11356)
                     /locus_tag="ON430_001290"
     CDS             complement(10280..11356)
                     /locus_tag="ON430_001290"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR3219216.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11552..13297
                     /locus_tag="ON430_001291"
     CDS             11552..13297
                     /locus_tag="ON430_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCR3219217.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13577..14014)
                     /locus_tag="ON430_001292"
     CDS             complement(13577..14014)
                     /locus_tag="ON430_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR3219218.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(13998..15371)
                     /locus_tag="ON430_001293"
     CDS             complement(13998..15371)
                     /locus_tag="ON430_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219219.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15693..16181)
                     /locus_tag="ON430_001294"
     CDS             complement(15693..16181)
                     /locus_tag="ON430_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3219220.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(16203..16805)
                     /locus_tag="ON430_001295"
     CDS             complement(16203..16805)
                     /locus_tag="ON430_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3219221.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4730 a         3608 c         3065 g         5446 t
ORIGIN      
        1 aatcaagttc tatccttttt gatatcttaa cttttggaag agagatgtaa ggctgctttc
       61 tacccatcat tcttactact cgaagcatag cgcagttgtc acaacctctt gataaacggt
      121 gtttaaaagc ttgcatctgc ggtaataagc ccaaccaaat taaaaacata agaagctgtt
      181 tttatttggt cgttcttatt acttgatgca gagcagcttt tttacaacct cttggtacac
      241 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaaa cggcaaaata tgaggaacta
      301 tttttccatt tttttctcta atactcaggt caaaacactg atttcacaac ctcttttttt
      361 aatgaaaaat atggctctca tgcgccgctt ctttgaattt cgtcattttt tcttcgatga
      421 acggatagat tgccgttcct acgattccaa agacaacaaa caaaccgatc atgatccaga
      481 ttgctttcgt agcatttggc caataaattc ctcctgcaga ttcccgtaac aagtttaccg
      541 catgagtaaa tggcaagaac ggattgatca tttggaagaa tttccctgag acttggatcg
      601 gatagttccc gcctccgcca gagatcgaca gtactaagat gataatagcg atccctttac
      661 cgacattatc gaataatgca gccaagacat agacgatcat catgaacgct aaggcgatca
      721 agacagcaaa tgacacgctg tagaccggat tttggacatc tactcctagt aagaaaatat
      781 tccctaatgt aacgatcaag ctttgcgcaa tcgccattgt caaaaatgtc agcatacgag
      841 caacgaattg ttctcgtttc gtaaaattgt ttcggtcttt ctttcctaaa tagtattctg
      901 tcgtagcaac acttgaaaga agaacagcgc cgacccacag acatagtgca gtataaaacg
      961 gggtgctagc tgaaccgtta tttgcgattg gataaagcgc attggtttgt agctcaacag
     1021 gttgtgtaaa gaagtcactt tcttttgttg cgtctagttt caataatttg atgacttcgc
     1081 ctagatcgac ttcttgttcg ccttttcgga tcgcgtctgc tgctttttgg ataccagctt
     1141 tgatatttgg ccagtcattg ttgatcaagt ccgcagcagc gttcacagct gtttcgactt
     1201 ctggcatttt ttcatttact gttgtcaatg taccggtgat ttcatttttt agtgctggcc
     1261 aatcattttg gataaaatca gcggccaatc ctaatttttg ctgaatgatc ggtaattcat
     1321 tgttgtatag gtctgcccct tcattgatgc cattgacgat ggtatccata tttccattca
     1381 gcattgtatt ggcatcatgg atttcttgtc caattgcagg catttctttt tgatattttt
     1441 ctaaaattgc cactgcattc gttacagttg ctttcgttga gctgagaagc gaatcaaagt
     1501 cgatctggtc cgctttgctc agtgcgcctt gtgcagtccc aatcgtatcg atgacttggt
     1561 ctagtagaga agtgacagtg gaactgattt gacctgtatt gataccagca atgatgccat
     1621 tgatattatt aacaacggca tcaatttgat ctagcaattc atcaatagcc ttcgtgtctt
     1681 cattttgaac agctgtgttc aaagcagata ggcggttttg cagattgctt agcaagggtt
     1741 ttaattgatt tagctgatca ataattggct gtaatttcgt atttccagca cttgtttgca
     1801 atttagtcag gaactcgacc agtttatcga ttgcattttg ttgttgagat attccgtcaa
     1861 taaagccgtt caatacattg ctgatgtttt tcttttcatc atcagtcagc ttaccatctt
     1921 caatagctgt tcttacagta tctattaacg aacctgtcgt tgttgccact tgtccaattg
     1981 acttcaatgt aacatctaca ctgtttgtga tgcttggtat cgcatctttc agctgagagg
     2041 cacctgattt tgtttggttt gctagatcac ttgcctgatt ttctaaattt ttcacatctg
     2101 gcaagatatc ttgtacttgt tggatgatcg tcaagccttg ttttgcttca tcgattcctt
     2161 ggttcattgt ctcttggatc gaagcaaaat cgccgtcgat ctctgctagt tgcttaccgg
     2221 cattttggat ctctgggatt ttttcttgca aatcaaggat gatttttgct tgatctttca
     2281 actcaggcat tttgtcattc aatgcaacga ctttttcacc catctcatct actttaggga
     2341 tgtaatccac gaattcattt gctttattca gtttttcctt gatttcaggt agttgacttt
     2401 gtaattcgag gacttgtttt gtgtagccgt cgatcgtgtc caaattttca tctgttgata
     2461 aaatcatatc tttaacttta ttgatactta ctaggttcgt atcgatgtcg taacctatct
     2521 cattgaatac tttcaataaa gtcgaactgg ctgttttgat aaattcattt gtgatttgcg
     2581 attggatcga acttgctcct ttatcggtga ttttcggtgc aatcgcattg attttttcat
     2641 ttaccgtata ttcgattttt ggtttcttga tatcaccact agtaaaactc agcagatctt
     2701 ctgagaaatc ctttggcaag taaatacctg cgtaatactt tccggaacgg actccgtctt
     2761 ctaattgttc ttttgaatca acgaattgcc atcctaattg tttattttta tgaagcgatt
     2821 cgatgacttg ctccccaatg gccacttctt taccttgaaa ctctgctggc ttgtctgcac
     2881 tgtaaacagc aattggcagc tctccagtat ttccatatgg atcccataat gctttgatgt
     2941 taaaccacgc atatagcgaa gggatgatca tgatcgcgat gatcagaagt gttgcaatcg
     3001 ggtttttgaa aatccgtttc cagtcaagca aatataatcg aagcgtattt ttgattgact
     3061 gcatagttct cccccttctt cttttttcag ctgaattact ttaccatgta tttcatactt
     3121 ttttcaatcg gcttttttca aaaaagtcga atttttggac atatcacggc aattgtctca
     3181 ttgtaatctc ttacatattg agaaataatt aaggattaca gcctttactt tatcatgaaa
     3241 taaaagggaa agataatttt atgccaaagt ttttatctat tatacgtata cgcaaaaaag
     3301 ctaaaacatt ttttgtcttt gcccctacaa aaatgtttca gcttttttcg ttttttattc
     3361 ctgtatttct tatttttcga atgacaggat gattttccca gcaggaggat gtccttcaac
     3421 tagctgatgt gcttggatga catgcttaag tgaagcagac agattcattg aaataaaaac
     3481 atgaaaggct cctttttggt aggctttgat tatgccagca aaggcttctg catctagata
     3541 ttcctttctt ggaacaaacg attcataaaa gccttccttt ttttgccgca aatctagatc
     3601 tgggaggtca tttaatgcaa catacctgcc gcctggtttc atgatctgga tgccggtttc
     3661 tcctttgata cttcctttcg tcgcatcgat tacaaggtct gcccgatcag caaattgcag
     3721 acctggattt gttttatcat aagcagcaaa tgctgaagct cctaattttt ttaccttttc
     3781 ctcattttta ctggatgcac tggttaagat tcggattccc ttttcatgga gaagctggat
     3841 cagacttgac ccgaccgcac ctgatgctcc tagtatcatt acagtatctg tcggctggat
     3901 ttccgtcaaa tggttgatta gattataagc cgtaattcct ggcgtgacca ttccggctgc
     3961 ttcttcccaa ctcattttat ctggaatttt tgctactttt gctgatggca acacgacttc
     4021 ttctccgtaa gtcccgccga ctgcatggac agctactcga tcacctacat gaacagttgt
     4081 gacatctgaa ccaatctctg tcacaatacc agcgccatca ttacctggta cataagggaa
     4141 tttcaatgtt cgtatctctt tcatttctcc taaacgcaag gcaacatcat aaggattcac
     4201 actgaatgct tttatttcta cacgaacatg tccagaaccg acttctctgg gatgagcatc
     4261 gatttcttca aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt
     4321 caccatctcc ctttttcttt aagtttacgt ttatgtcttt attttgacaa aaaaaatgtc
     4381 ttttcgtttt ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa
     4441 attatttatc taaaagcgta aagattacga tttacgctaa taaaaaataa ttggaggaat
     4501 ttgttgatgg ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa
     4561 cgctatgctg ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg cttgcgtaaa
     4621 cttcctttag atgctcgtcc agaacgattg aaaaatcgtg atttaatcga tggacgccct
     4681 cgcggataca tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt agcacataaa
     4741 ggtttgattc caggagtaaa aaaagccagc tggtaatcca agtctggctg taatgtaaag
     4801 gaggagggcg catggaccac tccttggatc atcaaaaaag atacgttcgc ttcttctagc
     4861 agaaatgctg gtactagaag cagacgtatc tttttttacg cacagctggt ccttttttat
     4921 atgtaaaggc tgcatggtta cttcgctata tttatttagt aacaggagtc agtgcttcga
     4981 tacccacact aattttcaat gctcggtcaa cttctgccat aatgggtcga tccaaatggc
     5041 agaccttttc tttcaatctt gatttatcga tcgttctgat ctgctccaac aggataacgg
     5101 agtctctttc tattcctgtt ccagtagctt tgatcccaat gtgagtcgga agcttaggtt
     5161 tagccatttt tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat
     5221 tattttgaat aaccaaaact gggcgtgttc cgccttgctc cgatcccacg actggagaaa
     5281 gatcagcaaa ataaatatct cctcttttta ccataggatc ctccttattc ttgatattct
     5341 cttggtatcc gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc taattgatct
     5401 gctatatcct gcatagtgat ttctttgtct gcatttttgc cgataagcgt cacttttgtt
     5461 ccgacaggaa attcttgagg caatcggatc atcagttgat ccatacatac acgcccaatc
     5521 gtttcacagt agttcccttc taccaataga gaaaaaccct gcatctttcg tggccagcca
     5581 tctgcataac cgatcggtat cgttccgatc cactctgctt caggagtgat atatgtttct
     5641 ccatagccga ttccttcacc tgctgggagt tttttgactt gaatcaattc agaaaccagc
     5701 tcaagtgctg gctgcaatgg ataaacttcc ggtagtgcgt gcccagaggg attaagtccg
     5761 tacattgcta ctccataacg aatcatattg ccgattgttt catcatgcca taaagccgtt
     5821 gcactattgc tgacatgtac atatcgagga agttcgggaa gttttttcag cacttctata
     5881 aagcgttctt tttgcagatt ccaatatgta tcatctgcct gatctgctgt agaaaaatgg
     5941 gtaaataaac cttcccattc taatacacga ctttctttga tgaatgcagc tgcctctttt
     6001 acttcttcag gtgtacaaaa tccgatacga cccatcccag tgtctgcttt tagatgaatc
     6061 gaaagaggcg tctctagctt acttttctct aaaacagcag ctgctgcttg caaccattcc
     6121 cgtgtacctg cagttactga taaatcatga gcaacagcta cagtcagtgc gtcgtaagga
     6181 accatattca agataaggat gggctgagta aatccagctt ctctcaactc gactccttca
     6241 tctaaatttg aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc agctgtttca
     6301 atagctccat gaccgtatcc gttcgctttt actactgcaa acaattcttt tccttgcgga
     6361 agtcttgcaa cttcatttgc tacattttca gttatagctt ttttatgtat cactgctttc
     6421 gttgggcggt gccaagcgac aaccatcgtt tttccttctt tctagtcttc caaaataatt
     6481 tgtgcaaaag ctgtttcatt tgtatgagta atactgaccc atacattgcc ttcatgaggt
     6541 gaacgcgtca caacaggctg accattttca ttttttaaaa tctcgacatc ttgaaaggta
     6601 acttttccga tcccagttcc ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg
     6661 cctaaaaatt ctacttggcg atgaaaaggc aaggactgga atagtttcat ttcatcagag
     6721 gtcaagattc gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat cctaggaagt
     6781 tcgactgcat cgatcccaat tccttttatc atatgctttt ctcttttcaa taaatttaga
     6841 acactctcat tctaccaaat tataaccatt ttgccacaaa taagtccaca aataacaaaa
     6901 atgaaagttt ctcttaaaaa agagaaactt tcattttatg tttaatcgtt atttgaacgg
     6961 attacaaatc cgcgtttttt atcattgtga cgtttaccgc catctttttg gctgttatta
     7021 tttttgctat aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta
     7081 cggttgtttc cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat
     7141 gggcgttctg gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt
     7201 agcaaaagtg caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc
     7261 aggtatttat ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct
     7321 aattgacctt tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc
     7381 gtcaagtttt caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac
     7441 ataccgcctt ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct
     7501 tgagggatat catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca
     7561 acgtctgtcg ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt
     7621 cgtttttgtt gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat
     7681 ccgcgtgcca attcgtctac acggcgtttt gtacgtccga agacgattgt taattcaggt
     7741 gtttgaacat caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca
     7801 tagtattgat cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga
     7861 tttttcatga atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt
     7921 aatgtttgtc tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc
     7981 atattcaaca tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat
     8041 gtgtggcggt taatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga
     8101 tctttcaagc cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt
     8161 acttttttgt cgcgccctaa acgatataat tcttcttgtg tttggatagc taattcacgt
     8221 gttggtgcga taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt
     8281 ggtaatccaa atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt
     8341 ccagctaatg ctagtggaat cgtttctgct tggataggcg ttgcttcttc aaaaccagcg
     8401 cgttcgactg atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc
     8461 ctcctatatg gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt
     8521 caaagtgtat tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct
     8581 gtggcaccac cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc
     8641 gttcagcttt atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca
     8701 gtccaatttc tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc
     8761 aagtttacag atttttattt catttgctga agttgttcta ccacttcaaa taagcccatg
     8821 ctgttgctgc cttttagcat aatactatct gttgctgcaa gattttgttt gatagcatca
     8881 atcaatttat tcttttcttt ttcaaaatag aacacaggta gttgggggta tttttggttc
     8941 aattcttctt ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca
     9001 ggatcgatat gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc
     9061 atatctgcta acacagcgat tctgcgtcct tttgttggca gatttttaaa agtatctaat
     9121 accagtccca tggctgttgg atttgcattg tagacatcgc tcaaaatagc tgcaccattt
     9181 cctgccgtta gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt
     9241 atctcaggta tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt
     9301 acattataac ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt
     9361 tgtgcttttc cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt
     9421 gtgactgttt gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca
     9481 acgagtaatc cgtctgatac aagtccgtca gtgatttcca ttttggcttt agcgattcct
     9541 tctcgcgacc caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt
     9601 ggttgagcca taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaaa
     9661 acgatctttt ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg
     9721 ttgtaatttc cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga
     9781 tctttcgtgg tggttttacc attgcttcct gtaattgcaa cgacttctgc gcctactttt
     9841 tccaagtaat aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact
     9901 ggaaaatttt ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt
     9961 gtgatcgctt gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa
    10021 cttccttcgg ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag
    10081 cattcgctgc ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct
    10141 aaaaaaggtt gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc
    10201 aagtaaattt ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca
    10261 gcctcttctt tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc
    10321 gattcattcc taactggatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt
    10381 cccataagag tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca
    10441 ggaataattc atttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta
    10501 acatcgtgta agctaacttc gcgtactctt gcgctttttg ataaacttct tctggcactt
    10561 cggctggaat ctgcatttcg attttattat tgatatattt tgcttcataa tcatagaatg
    10621 ctacgtcttt tacgacttca ccaggcaaag tcgtccgaac gtcttcattt cctaatacag
    10681 caacttcgat ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat
    10741 aggctgttgc taaagcattt tgcagctctt ctcggttttc tgcttttgta atgccgacac
    10801 tagaacccat attcgccggt ttgacaaaca tcggataaag caaagaacct tcacattgat
    10861 caaatacttt tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt
    10921 gcggcacacc agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac
    10981 tggtcaatac gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg
    11041 ttccatcttc cccgtttggc ccatgtaaaa ctggaaaaac gatggctcct tcttctttga
    11101 tttcgcccgg attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc
    11161 atgaaagatg caagacatct ttgctggcag gtttttctgt tagtaatgga cctttgaccc
    11221 attgtccttc ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat
    11281 ttaaaactga aaatgcggaa agaatggaca cttcatgctc tgcgctgcgt ccgccatata
    11341 gtaaagtaat cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc
    11401 atattttagc acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagccagctt
    11461 ctaaaagaac gagcgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa
    11521 aaagttatga taaagaaaag gagggatgat gatggcgcaa acaaatccac gcagccgctt
    11581 ggcagaaatc attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa
    11641 aaatcccgaa caggtaaaaa aagcctttga agaactaggc cccaccttca tcaagatagg
    11701 acagatgctt tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac
    11761 attacaagat agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga
    11821 actttctctt tcattatcag atatattcga tgatttttca aaatcgcctt ttgcctctgc
    11881 ttctatgggg caggctcatc gtgccaagct gaagaacggt gacgcggtag tcgttaagat
    11941 ccaacaccct aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat
    12001 ccctttgatc aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga
    12061 agtaaaacgt tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca
    12121 attctatcaa aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt
    12181 ttgttcgaaa aaagtgatcg tgatggaaga aatgtctggt aaaaacttga atcatttaat
    12241 gaacgccgaa aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca
    12301 agaagttgca aagcttttag ttgagaactt tatgaaacag gtgtttgatg atggtttttt
    12361 ccatgctgat cctcatccag ggaatctgct attccatgta ctaacaaaag aagaacagac
    12421 acaagctagc cgaaaaactg aaacagtcca tgaaaaagag ttcggttcct ttgcttttcg
    12481 agcctccact tctgcagaag accctgttgc gccatatacc atcaactaca tcgattttgg
    12541 tatgatgggg catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct
    12601 gtatacgaaa gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg
    12661 ctcgtttgat gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga
    12721 ttctcctatt gatgaaatca acttgcaaga agtattcaca catgtcgtga cgatttgtca
    12781 tcaaaataat ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttgggacatt
    12841 agaaggggtg atccgcgtcc tcgatcctga agtttccctc atggaagtcg cttcgccttt
    12901 tgctcaacat tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt
    12961 agacttattg gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt
    13021 agaaatgtgg acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt
    13081 attgagcagg atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt
    13141 gatcgtaggg tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc
    13201 cttattaggg atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga
    13261 tgcattgatc caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat
    13321 cagcttcaag aaataagaag gggttttaca taaattgttt ctcaaatttt tgtgaaaatt
    13381 cggcttattt ttcgaagagg ctacttctga aacaccgaga ataaaaaatg tgaaaaagct
    13441 gttctgcacc cagtaataag aacaatcaaa aagggaacat ggaacacatt tatcctgttt
    13501 ccctgttccc tttttgatgt attcgagcat aaataaaata ccttcgttac ctactatctt
    13561 ctgtacttgg taaaccttag tcaattgcct tttttaacgc ttgacgaaaa gacatatgat
    13621 ctttctccgt aaagggtttt ggaccctttg tccttatgcc agcttttctt aactttccac
    13681 tatgataacg ttgttctaga agcgtgccga tattcgcttc gtcaaattct tttccagagt
    13741 gatggaacac tcgcacaccc ttcggcaata caagtgaagc taaaccataa tcttgcgtga
    13801 tcaaaaagtc attcttctta accataccga cgattttata atccgcacta tcagctccgc
    13861 ggtctacgta gacgatttgg acatgattag cgtattcttt ggtcgtataa tggtccacac
    13921 tggtcacgat aaccacgtca agaccatagg attctcctaa ttttatgaca tcttctttta
    13981 ctggagaacc atctccatca atgaatagcc tcattctttt tcgccttctt ccataaatga
    14041 taaacagtta ctaatccgct cagtaaaaga ccaacattga tccattttgc caagataagc
    14101 agttgtacac tttctatcgg cactgattca tggttcagcg tgatcaataa tagatggatt
    14161 ggctctccca tttcttttgc taaaacaaac aaaagcgtca tcaataaaaa ctggtggatc
    14221 acactccctt tattttgagt agtatacttg acaacgatcc ggcgatccat tggaatagct
    14281 gtatcagttt gataatattc cttatttagc cagctgcctg ttactacgtg ccagaaacta
    14341 agcagaaaga cacctatcgc aaaaccattt ggaatcaaga ggaccaataa agaagaactc
    14401 agtccaagca attgcatagc caatggagac catgttaaac gtttcaaaat ccatggtccg
    14461 actatttttg ctgcaatcaa gcctgctgca taaggcaaat acataaaaat cgaaagtttt
    14521 tctaatccgt acagtatgcc aacataaaaa ctgctaaata aaaaaaggaa attccctagc
    14581 attccattta gaaaagtaaa cagatttagc cagcttggca acttccattc cttcttctgg
    14641 tgtcctaaaa caaacacgaa caagatgaac agcagcaaga aaccataaat agcaagatca
    14701 aaaagccttt catcgaataa caatcgggca tttctcaaaa gaaacaataa gccaaagaat
    14761 atcaaaaata aaacaagctc tcgcgtagct accagatttc ttttgatgtc tttgaaatcc
    14821 agttcgtaac gcggataatg actaaccgta tggtaggcca tgacgtaaaa aagtgtataa
    14881 atagtaaagc ttgctgcaat ctgagtcggt gcaggcaact caatcgcttg atacaggaga
    14941 aatagccaga tagctgcaaa taagtacttt ttcccatcca tattgataaa accttgcgtc
    15001 ttttcgtgaa aattgaccgt ggtattagca ggtacaagcc atgctgcact cagccctagc
    15061 aaaacacttg aaaaataaaa taaaggaaaa taaaatacgc ctaatattcc aagcaatgaa
    15121 ccagcaccac caatcagcag agcgatcatc agcaacgtgt aactatctag accaaatcgt
    15181 agactgttga ctaaaaatag tccagtcatc cgaaaggcat aaaacagcgt aaatggcaaa
    15241 atcgtcagcc aattatgcga gtctgttagt cccaaaaata aaagataagg aataaaaata
    15301 accgtgttac ttaagataaa gggcgttgtt tgtgtaaaat tacgcccaaa tcgtttgagt
    15361 ttttcgaaca tgacaatggc tccttaaaca gtcttataac taatctgctc ttcgatttta
    15421 gcattaaagg ggaaataaac caattttaca gaatatattc ttgtaaaaaa aaatacagaa
    15481 agaagcataa gcttctttct gcatttttac ggtacttgat tactcaagca gacaagccct
    15541 tctccacaaa agtcttgccc gacacccctg catccggcag gtgataaaga tagaaatcaa
    15601 ttatcctgtg cctccaaaat caattaggag ctctgtaaac cagagtcctt ctcgacccgt
    15661 cgcataaatt tactttaaca cagttcctat gtttatgcga gagctttttt cattttataa
    15721 tcgaccattt gaaatccttg accgatatct gaaataagtt cttctgtaat ctcaaatcct
    15781 tcatgcttat aaacctcgat tgcttgatga ttcccctgat tcactcgcaa aaagatctct
    15841 ctttcctttg tttgcgcagc aagttcttca taccagcgaa aaactgaaga agtcaatcct
    15901 tgaccacgta ctttatttag caaatataat ttacttaaat aaatttttcc atttatctct
    15961 tcataagcag tgtaaccgac agtcttttct tcgcttttta ataaaaagta ggacacacct
    16021 tctgtcagtt ctttttgaat ctgttttttt gactgataag ttttcagcat gtaatccact
    16081 tgtttttgac caatgatcgg cacaaaagct tccggccata cttcttgtat gactgccatc
    16141 agctcatcca gttcattttc tgttactttt attttttcca tacacccacg tcctttttta
    16201 cattataaat gaccttgcgc ttgatggaat aaaaagagca tgatcatgct atacacaccc
    16261 accgaccatg gtttcaagaa aatcacaatc tgcataaatt ccctaaaaga taatttcgtc
    16321 aaaccagcta ctagacagat caagtcgtct ggcgcaaagg gaagcagcaa ggtaacaact
    16381 agtacttttt taatatcttt cgtttttctt gtcagcaatt catcaaattt ctgatacttt
    16441 tttggcgata aaataagctg gacaaaggct acgccatagt aacggacaag caaaaatccg
    16501 attgcttccc caatcactag gccaatacaa ctatataaaa gcccaaatcc attgccaaac
    16561 atcagcatgc ccgctactga ggagatccct cccggcaaaa taggaatgat cacttggatc
    16621 acttgcaaca gaacaaatat catcactgca tacttgccaa attgctggat aaacgcttgc
    16681 aaagacgtgc tcgatcgaaa aatcccatga tgataagcat aaagaatcaa cacaaagaaa
    16741 aaaaacagac caataatggg catgatctga agacattttt gtaaaactga tcctttttta
    16801 gacatctata ttccccacat cttttttcat ttattaaaac tgcctcctt
//
LOCUS       DAJSMI010000050        16796 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJSMI010000050 DAJSMI010000000
VERSION     DAJSMI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.050"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            259..1695
                     /locus_tag="ON430_001296"
     CDS             259..1695
                     /locus_tag="ON430_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR3219222.1"
                     /translation="MEACARFFIKENCCFLFQSPLSEEWLRIFHKNGYQGTTQLNKKL
                     VYHTALVLGGGGAHGAYQIGVWQALKEHDINFEIITGTSVGALNGALILQGDMKKAVN
                     LWKKLSTRQVLALPEMAAVEDLRERFYRERRQMTKTALIEGGVSSAPLEKMVKDNLDL
                     SLLKHNPKIRLYTVSTKLPELAEVVTDIQQTKTEEIPDWILASASFYPAMAYRKIGKN
                     KYADGGYRNKIPIDIAINKGATEAFVVDVQGPGPAKKIRMPDIFIHWKCQTLWTLGSF
                     LLFDSQRNQLNLQLGYLEMKKRLGYYYGNWYTFHSVKPARTYWRGFLSYLTKEIQLEL
                     SFFKSIKFWQKLRNLYKNRVVPETCGLAMLELLAKKHFLLPNEIYQVDMMIQMICQQD
                     IKSMPDDLLAQIGQLSTEEWRRYRKYQQKIQNERTKTVYFDYLLQGKRKDRLQHELLK
                     QPVDTLLILYLYYLKEEQPWHKNFHMKS"
     gene            1665..1883
                     /locus_tag="ON430_001297"
     CDS             1665..1883
                     /locus_tag="ON430_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCR3219223.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            1903..2793
                     /locus_tag="ON430_001298"
     CDS             1903..2793
                     /locus_tag="ON430_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR3219224.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2811..3095
                     /locus_tag="ON430_001299"
     CDS             2811..3095
                     /locus_tag="ON430_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219225.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            3582..4196
                     /gene="gmk"
                     /locus_tag="ON430_001300"
     CDS             3582..4196
                     /gene="gmk"
                     /locus_tag="ON430_001300"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR3219226.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4201..4506
                     /locus_tag="ON430_001301"
     CDS             4201..4506
                     /locus_tag="ON430_001301"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR3219227.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            4759..7167
                     /gene="priA"
                     /locus_tag="ON430_001302"
     CDS             4759..7167
                     /gene="priA"
                     /locus_tag="ON430_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR3219228.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7182..7673
                     /gene="def"
                     /locus_tag="ON430_001303"
     CDS             7182..7673
                     /gene="def"
                     /locus_tag="ON430_001303"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR3219229.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            7666..8604
                     /locus_tag="ON430_001304"
     CDS             7666..8604
                     /locus_tag="ON430_001304"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR3219230.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            8604..9962
                     /gene="rsmB"
                     /locus_tag="ON430_001305"
     CDS             8604..9962
                     /gene="rsmB"
                     /locus_tag="ON430_001305"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR3219231.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            9975..10715
                     /locus_tag="ON430_001306"
     CDS             9975..10715
                     /locus_tag="ON430_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCR3219232.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            10712..12781
                     /gene="pknB"
                     /locus_tag="ON430_001307"
     CDS             10712..12781
                     /gene="pknB"
                     /locus_tag="ON430_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCR3219233.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            12946..13845
                     /gene="rsgA"
                     /locus_tag="ON430_001308"
     CDS             12946..13845
                     /gene="rsgA"
                     /locus_tag="ON430_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCR3219234.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            13858..14508
                     /locus_tag="ON430_001309"
     CDS             13858..14508
                     /locus_tag="ON430_001309"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR3219235.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            14509..15147
                     /locus_tag="ON430_001310"
     CDS             14509..15147
                     /locus_tag="ON430_001310"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCR3219236.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15302..16156
                     /gene="mreC"
                     /locus_tag="ON430_001311"
     CDS             15302..16156
                     /gene="mreC"
                     /locus_tag="ON430_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR3219237.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVASVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16160..16669
                     /gene="mreD"
                     /locus_tag="ON430_001312"
     CDS             16160..16669
                     /gene="mreD"
                     /locus_tag="ON430_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR3219238.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
BASE COUNT         5627 a         3032 c         3674 g         4463 t
ORIGIN      
        1 ataaaaaaag tttacttttc acagaaaatg tcgctgtatt cgcctatcca gcaagcaaaa
       61 caaccccttc cattttttca aagtcatcaa gaaagtagag acgctttttg gcgttctgca
      121 aaaaagcaat gtgcttttaa agcagtatcg aaaaaatata ccgtctattt tactattgct
      181 aaaagtagag gagatgttat ttttaataat ctgttgattg aaggtgaaac ttttgaatgg
      241 aaaaaatttt ttcggtatat ggaagcttgc gctcgttttt ttattaaaga aaactgttgt
      301 tttttatttc aatctcctct atcagaagaa tggttgcgta tttttcataa aaacggctat
      361 caaggaacca cacaattaaa taaaaagctg gtctatcata cagcacttgt tttaggtgga
      421 ggaggagcac acggtgctta tcagattggt gtgtggcagg cgctgaagga acatgatatc
      481 aactttgaga ttatcacagg tacctcggta ggtgcgttga acggcgcatt gattttacaa
      541 ggagatatga aaaaagctgt gaatttatgg aaaaagctgt cgacaagaca agtgcttgcg
      601 ttaccagaaa tggctgcagt tgaagacctt cgtgaacgct tctatcgaga aagacgccaa
      661 atgacgaaaa cagcactaat tgaaggcggg gtttcttctg ctccattgga aaagatggtc
      721 aaagacaatt tggatttgtc cttactcaaa cataatccca aaatacgttt atatacggtg
      781 tctactaaac tgccagaact agcggaagta gtgactgata tccaacagac aaaaacagaa
      841 gaaataccag attggatact cgcctctgct tcattttatc ccgctatggc ttatcgaaag
      901 atcggaaaaa ataaatatgc agacggtggg tatcgcaaca aaatacctat tgatattgcg
      961 attaacaaag gagcaacaga ggcatttgtc gtagatgtgc aagggccagg cccagcgaaa
     1021 aaaattcgta tgccagacat ttttatccat tggaaatgtc aaacattgtg gacactaggc
     1081 agctttctgc tttttgatag ccaaaggaac caattaaatt tacaattagg ctatctggaa
     1141 atgaaaaaac gattaggcta ttactatggg aactggtata catttcattc tgttaaacct
     1201 gcaagaacat actggcgagg atttttgtct tacttaacaa aagaaattca gttagaatta
     1261 tcttttttca aaagcataaa attttggcaa aaactgcgta atttatataa gaacagggtc
     1321 gtaccagaga catgcggatt ggcgatgcta gaacttttag caaaaaaaca ttttttactg
     1381 cccaatgaaa tctatcaagt agacatgatg attcaaatga tctgccaaca agatataaaa
     1441 agtatgccgg atgatttatt ggcacaaatt ggtcaattaa gtactgaaga atggcgaagg
     1501 tatcgaaaat accagcaaaa aatacaaaat gagcgcacga aaactgttta ttttgattat
     1561 ctgctacaag gaaaacgcaa ggatcgtttg cagcatgaat tgcttaagca accagttgac
     1621 acattactga tcctatattt atattatctc aaggaggaac aaccatggca caagaatttt
     1681 catatgaaat cgtagaagaa atcgctatat tgtcagaaaa caacaagggg tggcgtaaag
     1741 aactgaacct agtcagctgg aatggtcgcc cgccaaaatt cgatcttcga gattgggcgc
     1801 cagaccatga aaaaatgggg aaaggcctga cattgacgaa cgaagagttt gaacaactgc
     1861 aaaaagcaat agaaaatatg taatgaggaa ggaccctgtc gaatgaaaag tatgactggt
     1921 tttggcaaag ccatccgcga aacaaaagcg tatcagttag atattgaaat aaaaagtgtc
     1981 aaccagcgtt ttctagatgt tcaaatacga agtcctaaaa tcctgaactt tgttgaaaac
     2041 gattttcgtc agcttattaa acagcagtta agccgcggtc gaatcgaagt atttgtgaat
     2101 ctagcttata ttggcaaaaa cgaaaaacag atcgtcatca actgggaatt gattgattca
     2161 cttatcactc aattgacaga cggaattcaa caacgatacg gtagtcaaaa tcaagtgtca
     2221 attagtcgga tggcggaacg cttcgcgctg gacgaaaact tcgtaacggt agaagaaaaa
     2281 ccggaagaga gcatggaaga attggcacag ttagctctag atacattgaa aagcgcactt
     2341 gattctattg aggcgagtcg agaaaaagaa ggtcaagctt tagctttagt gttgaaaaag
     2401 aatactgaag aattcaaaga agtactaaaa aaactcgata gttttgtaga aatctatgaa
     2461 aaggaatttc aaataaggta tcaaaaaaag ctggaggaat ttcttggagc gaaagtcgat
     2521 gagcaacggt tgttgacgga gctagccatc ttgttggaac gcggagatat tcacgaggaa
     2581 ctggatcgtc tggcgatcca tgtacaaaaa ctagatgaac ttatcgaagc acaatgtcct
     2641 gtaggtcggg aattagattt cctgatccaa gaaatgaatc gggaaatcaa cacgatcggt
     2701 tccaaatcaa atgcaatcga aattaaaaat caagtcgtcc agtctaagac gatcttagaa
     2761 aaaatcagag aacaagtaca aaatattgaa tgacgaaaga ggtagagata atgaaatatt
     2821 atgacgtaac gttccacgaa ttaagtggaa aaactgtggt taaacgggat atcccttctg
     2881 aaaaaaacgg ctttgacgta tggaaagatg cctgtgcaga ctataatgag aacgaactgt
     2941 ttattttgat caacgacggt gcttacgtga cgatgaatcg gaaatttatt gttcgaattg
     3001 acacagaaga ggtagaagat ccgacagaaa aagcacgttc tcgaaaagat gagatcatgg
     3061 gtgtagtgaa tactttgtcc aatatgggct tttagcaagg atgctgaaca aagaaataca
     3121 ataaataatc agaattggat ttcttcgctt agcttctttg ctctaggaat acatttaaag
     3181 gttgtgacaa aattcgtgtc acaatctttt tttattttga ataagtgatg ttcaaaaacc
     3241 attttctcgg tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt
     3301 ttcttcatac tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc
     3361 ttgcatctgc gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc
     3421 tgttcttatt acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt
     3481 ttaccaattt ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa
     3541 taaacgataa gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat
     3601 taatcgtgtt gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg
     3661 agagtgagga taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag
     3721 gggaggtcga aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg
     3781 aagctggaga gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt
     3841 catatgtcaa caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag
     3901 gagcaaaaca agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag
     3961 atttagcgga attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg
     4021 aagaacgaat gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg
     4081 ttgtaaatga tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac
     4141 attttcgagt agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt
     4201 atgatgttaa aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta
     4261 gtgattttag caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat
     4321 tcttttgatt cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta
     4381 gtcaatgatc cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt
     4441 caagcacaaa aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa
     4501 atgtaataaa gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt
     4561 ttcaaacatt cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag
     4621 ttcttttttt tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa
     4681 tccatcgatc tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa
     4741 aaaaggagga aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg
     4801 cagaccgatc agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc
     4861 gggatgcgag tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg
     4921 gctatccaaa aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta
     4981 gaccttgctc cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt
     5041 acttacgcat tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa
     5101 tatgataaat tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag
     5161 cgagaagtga tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc
     5221 tggcgccaag agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa
     5281 acgattcgtt tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa
     5341 ttacggcaaa atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa
     5401 gaagaaccaa ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga
     5461 aaagaaaagg gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac
     5521 cgagtatttg accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa
     5581 caaatcatca cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact
     5641 ggcagcggga aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa
     5701 acggctatta tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa
     5761 ggacgcttcg gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat
     5821 gatgaatggc gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct
     5881 atttttgccc cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt
     5941 tacaaacaag atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa
     6001 tatcatcatt gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga
     6061 gctcaaaaag gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag
     6121 cttccagcta ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact
     6181 ttttctgcta atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc
     6241 ctactgctca atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt
     6301 cttccatgtc ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc
     6361 tgccattatt gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat
     6421 aaaatccgct actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat
     6481 ccgcaagcta ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa
     6541 cagattttac aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt
     6601 gctaaaggtt tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc
     6661 ctgaatttac cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca
     6721 ggacgagcag ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa
     6781 cattacgcca tagaattggc taaaaaacaa aattatgagc agttttatca gcaggaaatg
     6841 catgtacggc atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc
     6901 catcctgaag aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa
     6961 gtgttaagcc ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa
     7021 aaccgatatt attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt
     7081 ttggatgaaa ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt
     7141 gacaacgaac cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga
     7201 tccaccctaa tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga
     7261 caatcgcttt attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg
     7321 ctgctcctca agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga
     7381 aatttgaact gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag
     7441 gttgtctcag tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc
     7501 gttattatga tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg
     7561 ccttccagca tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc
     7621 agatcccgga agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta
     7681 tttatgggca cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac
     7741 gagatccagg cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg
     7801 ccgacaccgg taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa
     7861 atctccggtt cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact
     7921 gcggcatttg gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc
     7981 aatgtccatg cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc
     8041 atgaatgggg agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca
     8101 ggcggaatct atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg
     8161 tttgaaaagc ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg
     8221 gatggacaaa aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca
     8281 cgggaacaag aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc
     8341 ggaatgcgtc cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta
     8401 tcggttgaag tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac
     8461 aaaaaaaatc tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag
     8521 ccagctggta aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt
     8581 cagattggtc agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac
     8641 gatatatggc attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt
     8701 tgttgaatga gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag
     8761 aattgatcta tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga
     8821 tcgctaaagc aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc
     8881 aattagaatt tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg
     8941 caaaagtaaa aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc
     9001 agcgtcaagg agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca
     9061 aaatcagcct gcctctatgg atgactaaac gactggtgga tcaaatcgga tacgaagaaa
     9121 ctgaaaagct cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac
     9181 gccgactttc cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa
     9241 aaagccaagt cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat
     9301 tgttccacga tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta
     9361 tgcaaatcga gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga
     9421 cgcatattgc ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac
     9481 acgaacacaa agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg
     9541 tctacgctca aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg
     9601 atcggatttt agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata
     9661 ttcgttataa aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt
     9721 tagaaagctg cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga
     9781 tcttaaaaga agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg
     9841 agaggattga tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga
     9901 ctttgtatcc gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt
     9961 aatgaggtga aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa
    10021 atcaagacta tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag
    10081 acgggatggg tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag
    10141 gcgaagcttg ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc
    10201 aatcgattca agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata
    10261 acggtatggg aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca
    10321 atgttgggga tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg
    10381 atcattcttt agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga
    10441 accatccaag gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag
    10501 atgtttctac tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga
    10561 cgaacatgct gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc
    10621 ataaagtaac tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg
    10681 ttcttcttat cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg
    10741 ccgttatcaa atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca
    10801 cgatttaatc ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga
    10861 ccagactgcg attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc
    10921 aaacattgtt agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga
    10981 atacgtcaaa ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga
    11041 aacagcagta aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca
    11101 aatcatccac cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa
    11161 aatcacggat ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac
    11221 gatgctagga tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa
    11281 gcagtcagat atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc
    11341 gtttgatggt gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc
    11401 tataaaagca cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac
    11461 ggctaaagaa cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac
    11521 agtattatca ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga
    11581 aacaaaagtt atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat
    11641 gcctctgcca aaagacaagc cagaaaacga aaccttacca gaggaggaag aagttctgcc
    11701 tgatcctaaa aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt
    11761 tttaggaatc ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc
    11821 tgatgtttct gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga
    11881 agtagcaaca aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa
    11941 gacagacccg gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag
    12001 tagcggcacg aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga
    12061 agcaagtttg aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag
    12121 ttcagaggta gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga
    12181 tcctaaaaca gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag
    12241 tagtcatgta ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa
    12301 tatcaataat attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat
    12361 cagtcaggat ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt
    12421 aagccaagga acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca
    12481 aagttattta gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa
    12541 cacggtggat aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa
    12601 aggaatggtt gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga
    12661 aaaagactct tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc
    12721 aaaagaagaa tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata
    12781 agtgctgaat aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt
    12841 gtgtttctat ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat
    12901 aacacctact ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa
    12961 ggacaaatca gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat
    13021 caaacaagag gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag
    13081 gtgatctttg agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa
    13141 aatcaattag ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc
    13201 gtagagcctg cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag
    13261 tcgatcgaac cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc
    13321 gaagagatca agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg
    13381 ggtgatacga cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag
    13441 tctggagcag gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca
    13501 ggagagattt ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt
    13561 cctatctatg gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca
    13621 attgaagcag gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc
    13681 aaatttagag agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa
    13741 acaggtggta tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa
    13801 aaaagaaaac caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg
    13861 aagatagcac catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta
    13921 gtggaaaaag gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca
    13981 aatatcacat tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta
    14041 gacgtccatc tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga
    14101 gcagatatca ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg
    14161 acaaaaagct taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg
    14221 atcaaacatg ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc
    14281 ggcggacaaa gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt
    14341 agagagaaca attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca
    14401 aaaatctgta aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc
    14461 gaaaatccag tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta
    14521 ttagctgctg gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc
    14581 tatgtcggga tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg
    14641 gcagtgggtg attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc
    14701 aaagagtata cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta
    14761 aaaacatttg aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga
    14821 ttggatcatc ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc
    14881 catcagctta ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat
    14941 atagtaagac agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg
    15001 aatcttacat taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca
    15061 tcttatgcaa gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg
    15121 attgcagtta ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg
    15181 ctaaaaatga cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt
    15241 tgtttcacgg catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt
    15301 tgtgaagaag ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt
    15361 ggtgacgata ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt
    15421 ccaatccatc gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg
    15481 gtgggtacga aatggagtag cttctgtcca taatcttttt tcaacttatg ccgaaaatga
    15541 gcggctaaaa gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga
    15601 gagagaaatc gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga
    15661 aaaagtaacg gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt
    15721 agacaagggt tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt
    15781 gattggccga gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc
    15841 taatgaaagc tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg
    15901 tttgcttaaa aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga
    15961 tacggatatc aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc
    16021 taacttgccg atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga
    16081 agtttatgtg aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg
    16141 gctagcggag gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt
    16201 tttgttcttt ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa
    16261 aggcacctat atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc
    16321 gtttgaaaaa cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta
    16381 ctatattggc gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta
    16441 tgtaatgaaa gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt
    16501 tgtcacgggt tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt
    16561 aaataacacg tattttatta caaggttttt aggacctaca ctgctgttga acatgattat
    16621 atttgtatta ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt
    16681 atttttaggt cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc
    16741 ggctttttca tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgtt
//
LOCUS       DAJSMI010000051        16783 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJSMI010000051 DAJSMI010000000
VERSION     DAJSMI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.051"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(29..574)
                     /locus_tag="ON430_001313"
     CDS             complement(29..574)
                     /locus_tag="ON430_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3219239.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(586..1278)
                     /locus_tag="ON430_001314"
     CDS             complement(586..1278)
                     /locus_tag="ON430_001314"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR3219240.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1297..1581)
                     /locus_tag="ON430_001315"
     CDS             complement(1297..1581)
                     /locus_tag="ON430_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219241.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1594..2157)
                     /locus_tag="ON430_001316"
     CDS             complement(1594..2157)
                     /locus_tag="ON430_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3219242.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2385..3047
                     /locus_tag="ON430_001317"
     CDS             2385..3047
                     /locus_tag="ON430_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCR3219243.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3171..4367
                     /locus_tag="ON430_001318"
     CDS             3171..4367
                     /locus_tag="ON430_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCR3219244.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4468..5844)
                     /locus_tag="ON430_001319"
     CDS             complement(4468..5844)
                     /locus_tag="ON430_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3219245.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPKNGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5881..6747)
                     /locus_tag="ON430_001320"
     CDS             complement(5881..6747)
                     /locus_tag="ON430_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCR3219246.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6836..7861)
                     /locus_tag="ON430_001321"
     CDS             complement(6836..7861)
                     /locus_tag="ON430_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3219247.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7879..8553)
                     /locus_tag="ON430_001322"
     CDS             complement(7879..8553)
                     /locus_tag="ON430_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3219248.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8737..9630)
                     /locus_tag="ON430_001323"
     CDS             complement(8737..9630)
                     /locus_tag="ON430_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3219249.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9704..11401)
                     /locus_tag="ON430_001324"
     CDS             complement(9704..11401)
                     /locus_tag="ON430_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3219250.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11394..12707)
                     /locus_tag="ON430_001325"
     CDS             complement(11394..12707)
                     /locus_tag="ON430_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR3219251.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMIDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12707..14635)
                     /locus_tag="ON430_001326"
     CDS             complement(12707..14635)
                     /locus_tag="ON430_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219252.1"
                     /translation="MAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETKMPILGDE
                     TGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYTSGSRVGG
                     PEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNINKLIEGSN
                     MPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGE
                     KVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLYDNAIGTI
                     QLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIR
                     KVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEY
                     EVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLE
                     DELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASD
                     LLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLKVEISYYP
                     NEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILLAAFFMAL
                     AGAAYGLFVRQSKKEGAR"
     gene            complement(14762..16734)
                     /locus_tag="ON430_001327"
                     /pseudo
     CDS             complement(14762..16734)
                     /locus_tag="ON430_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339119.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
BASE COUNT         4734 a         3780 c         2897 g         5372 t
ORIGIN      
        1 ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa
       61 aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggca tctttgaaaa
      121 tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg
      181 tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat
      241 ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct
      301 catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca
      361 cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat
      421 caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct
      481 ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat
      541 catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt
      601 gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa
      661 gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat
      721 attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc
      781 agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt
      841 aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact
      901 gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc ctgggttgta
      961 gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc
     1021 tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc
     1081 gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg
     1141 tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc
     1201 ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc
     1261 gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa
     1321 aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag agttccattt
     1381 ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc
     1441 tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg
     1501 ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt
     1561 aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca atagttccca
     1621 gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg
     1681 cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg
     1741 attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc
     1801 tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc
     1861 ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc
     1921 agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag
     1981 acgatcttgt tcgtcaaaag caatgatccc gacaccgcca ggatgaaata ccagttctct
     2041 cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc
     2101 gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc
     2161 ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa
     2221 actccttatt agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc
     2281 tttagtccga tactctttct agtagtccgt atgctaaaat aagtatgtaa tcgaataacg
     2341 aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt
     2401 ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt
     2461 ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc
     2521 gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact
     2581 atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa
     2641 caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa
     2701 tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag
     2761 aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg
     2821 atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg
     2881 aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt
     2941 atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac
     3001 agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat
     3061 aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta
     3121 gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata
     3181 aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca
     3241 acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc
     3301 tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac
     3361 aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg
     3421 gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag
     3481 cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag
     3541 ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg
     3601 tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca
     3661 agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag
     3721 aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg
     3781 gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag
     3841 aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc
     3901 gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac
     3961 cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta
     4021 tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc
     4081 aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga
     4141 aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa
     4201 tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga
     4261 cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac
     4321 acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct
     4381 tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta
     4441 tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc
     4501 atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac
     4561 agtttgcaga cgaagcatca atacaataca agtcaagatg gagacgattg gcaatagcgg
     4621 aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag
     4681 taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa
     4741 tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc
     4801 ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc
     4861 cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag
     4921 tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct
     4981 cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa
     5041 gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct
     5101 tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc
     5161 caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat
     5221 gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat
     5281 gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag
     5341 cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc
     5401 atttttcgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa
     5461 aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc
     5521 cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata
     5581 ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga
     5641 caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg
     5701 attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag
     5761 catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat
     5821 ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga
     5881 ttaaactgtt ttcatatacc ctaaaaggat accacctaaa atgactaaaa ggcaaccaac
     5941 aataacataa atgagctctt ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa
     6001 agtagagata atgatcccca tttgtgaaag tgagaagcca acagctaaac caagtgattt
     6061 gactgttaga agcatgcaga tgtttcctaa gccccataat aatccacaga tcatgttttt
     6121 ccacacaaac tgatcgactt taactttacg gaaagcaaag aagcttgcgc ctaagatcat
     6181 tccgatactt tgaggtaaga taattgacat cgcatccaaa ttcgcccagt tgacgatgat
     6241 ggtatataca ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc
     6301 aagcatttta ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc
     6361 accgcttaca agaagagcta aagcaataat accgtataca taatctttcg tttgtgtcca
     6421 ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa
     6481 tggtagccct acagaaacac ccatgtactt cattccatgg aactgtccat tttgtccaac
     6541 actccaagct aagccggata aaaatccgat caagaaaatc caaggggtga ttacaggact
     6601 aacaacgaaa aagacaacta aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg
     6661 ttgattggca tcgccaccaa ttttaccact tactaaacca atactacccc atgccaacat
     6721 agggactaat gctattaaaa tttccataac caatctcctt ttatttcaca tgataagaaa
     6781 cttatcagcg aaaacgttaa caacacaagc actttttttc gtttctaacg attttctaac
     6841 gtttttcatt ataaaaattt aagattggga aaaataaagt cattgttgtc ccttttcctt
     6901 cagttgattc agccgtcaaa tggataccta atttttcggc cagatttttt gctaaataaa
     6961 gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt tctcccagta aatcccttat
     7021 caaagatccg tttcaagtct tccgctggga taccgatccc tgaatcttta actgaaaggc
     7081 tgactccttg cgcttccttt tctatgctca caatgatgtt tccgtgatcc ggcgtatatt
     7141 tgatggcatt actaagcaat tggttgaaga taaaagcgat ccatttagca tcggtcaaaa
     7201 cagtatgatc ctcaccaacg atctcgtagc gaatattttt ttggataaaa taatttgcct
     7261 gactgcgtat cacaggctga atgattcctt ttagtccata ttcttggatg aggtagtccc
     7321 gcgagaaact gtcgagtctt gcataataca aaacttgttc tacgtattca tcgatttttg
     7381 ataattcatt ttctagtaat gtatactttt gatcgtcaat atcgaactcg atcgaacgaa
     7441 ctagcaattt agaagcggaa agtggcactt tgatttcatg gacccatgag tcaatataat
     7501 ctttttggtc ttgttgatta ctgattgctg attgcattac ttcttggtgt tcacggatca
     7561 gctgattgat atattcttct accagttttt cttcttctgt cctcccacca ctcaaataat
     7621 tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca
     7681 agtaaccacc cagcagataa aaaatcagaa agacgccctc aatcaaagct aaataagcaa
     7741 ttgtgctcaa atcaactttg atgtttggcg ccagccacat cacaaagatc gtcaatgcaa
     7801 taaaaaacag ccatcccaag atcaatgtcc actgatcttt caaatattta aaaaaattca
     7861 tcttaaccgc tcatcctttc agggaacgat atatccctgt cctacttttg tttcgatata
     7921 tccttggata cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt
     7981 attgtcatct acaaatcgtt cgtcatccca cagtgctcgc agcaattttt cccgacttaa
     8041 gatctttcca ttgtgcttca tcaaaataaa taatagacga tattcatttt tgctcaaatc
     8101 aatgatctct ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg
     8161 gctcatcatt tcactagata attctgtgta gttgtaagaa cggcgaagta atgcattgat
     8221 ttttgcaatc agtacatcga tttggaaatg ttttgtcaca aaatcatctg cccccatgtt
     8281 catagccatg atcatatcca tattcgtatt cctgctggag ataaaaatga tcggtacttt
     8341 cgatacttca cgtatttttt gacaccaata atagccatcc aaaacgggaa gattaatatc
     8401 tagtaataca agctgtggtt cttctctttc aaagtctgag aacacttgat tgaaatcggt
     8461 cgttccaaag gtttcaaatt gccatttctc aagttcctcc atgatcaatt gtcgaatgat
     8521 ctcttcgtct tcgactacca tgatttttgc cataaaaaat ccctccatct ttcttcttgc
     8581 tactacaata tcaaagaagc ccttgtgaaa caagttggct tacctggaaa atgtcatttt
     8641 agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag
     8701 cttaaattta ttatgttatt cttaataatt ggtctattat ctgttatttt tctttcgttg
     8761 tttttgtctt ttaaattttc gataacacca ataaccgaaa agcgttagca ctagcaatat
     8821 acctagaagg agtataagta atcgataaag atggtatttt tttgtctgtt ctatctttga
     8881 agagacttct tctgtaaagg gaactcgtct tcctgtcact agtaatcggt gagtgttcac
     8941 ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc ttttctctaa ttgctaatgt
     9001 atttgttttt gttggaagga caacaatttt ctcaacgact tcataagcga gcatccgacc
     9061 atacacttca agatagaatc ggtcatcttg ctccatttct tcaagatccg taaagagttt
     9121 tttctcagca ataccgctat gggcggtaat cacggaatgt gtactctctc ctccagttgg
     9181 aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc
     9241 aaataaaggc aagctaacat tgattttcgg aatatagatg gctccgatca tatgttgttc
     9301 gtaatattct tttcctggat tttggacatt tctaaccgct tgctcaaaag gatctttgac
     9361 ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc tgatttttct tttcatgcac
     9421 tgctaattgt tgctcatgtt tttcttcttt atcgcgttca agctgctttt gataattctc
     9481 gatccttctt tgatccaaat aattgctgag agcatctgct aaaaatggat atgtaaaaac
     9541 tgcagctccg gcaagaaaca tcactgccat aaataattgt aataaaagac tgatttgggt
     9601 ttgaggtcta ttacctattt tctttttcat tttcttcacc ttcttattta aaaaaactgc
     9661 agcaaccttt ataaaaccga ttgctgcagc ttatttttgt tgcttatact tcagacttca
     9721 ttttagattt acggtaccag ctgtacgctc caatcatcaa acttattccg attagtaaga
     9781 atgccaagag cccgttccca ccagtagcag gaagtgaccc gcgtttcata ttcggtactc
     9841 tttctggccc tggattttct actcctacga tctgaatttc ttcattatag cttccataag
     9901 agatgataaa ttcagtaaag gctgcctcac ctgttggaag tacataacct tctggcgcca
     9961 tggtctcttt catttgatag gttccataat ctaacccttg aacctcaaag tatccatttc
    10021 catctgcgac aaattctgtt gcttgtccgc ttgttgccca ctcaattcta tctgctcctg
    10081 attcataaac agctaaaggc gactcccctt tataaaatat tgcgtattca gaacggtctt
    10141 cattccacaa ttcgaatctt gcatctttaa gcaattcact gcttgatgca tcatattttt
    10201 caaactttct tccacctgtt gtgactttct ctggcggctc tactccttct tcataatcaa
    10261 attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca tccgcttcta
    10321 gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt gcatctgcta
    10381 atctacctaa agtatctact ttggctactc cattttcttc tgtcaaaatc gtcacgacaa
    10441 acccgttgct tactacttct actatataat cttgtccttt tgttaaaatc gtctcgccgg
    10501 ctcgcacttc tatttcctga tcataataag ttagaccaac tgtaggcgta tctgtgattt
    10561 tgtaattttt gataacagcg cttccgtttt caaggacaga atcgatgaaa taaggaatag
    10621 gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag atcaatataa gaaatcgttt
    10681 cttgttctcc agcttcatta ataatcgtta cttgtctaag gtcgctttcc tctacattca
    10741 tctgtttatc tgcttctctg atttcgtttt tcggatacaa atgcacatct gtattgtatg
    10801 tgtctttcca tgtagaacca tcccacacgc catccaccat atcaggcatc atcactggca
    10861 tcataacaac cattggagac gcgattctgg aaatatgagc tggcgaataa gtttctaaga
    10921 aaagatagac tttatctcta gaagaaggtg cttcttccgt cacttggacg gtagagaata
    10981 ccgcttctcc agcttcattc gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa
    11041 accaactttc tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt
    11101 cttctcgttc cggtgttaaa ggattatctc gaatcaaatc gtaatatact tccgttacgt
    11161 caaacaaatc aaaatcgact cctggaatat tttcttcacc aaaatccgta gaaaccaatc
    11221 cgctattcgg tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca
    11281 cttcacttgg tttttgtgcg acttcttcgg ctttgactag ttttccgttc ccgaaaatcc
    11341 ctattcccgc agtaacgagt aacagtacac tcattcccgt gacaatcatt tttttattca
    11401 tttttttctc cccgttcttt ttttattaat aaaacagcac ctaacaataa aagaattcct
    11461 agtaaagaaa tcaacgttct tgcttcattc gtttgcggga atacggacca ttttcctgat
    11521 tgacctggat tgcttggacc actcggctct ttcggtttgt ccgttgtttc ttttacggtc
    11581 tcgttataaa cacgtggtgt tgcggattca taggcagaga ctggaacttt cccggattct
    11641 aattcagcca tctcctgtcc gttaactact acatattgtg gttggccttt tgcatccacc
    11701 caaaattcag ggatgatgac ctcgattctc ttacttgctt catccatctc gtaaccttcc
    11761 acacctttta attcttcaaa ataataggtg ccagaaggca agaaacgttg tcctgtcgta
    11821 acaaggccct ctttatccga aatgaatttg cttacatttt tgtcattcaa aggatcattg
    11881 tctgctggtt tgatccattg atttttcaag tcattagcag gagccatatc taaataatat
    11941 ttcccccctt ctatcatctg atatagagca aaaacagcac cttctaatgg ttttcctttt
    12001 tcagttgttc ctctttgacg tccatacttg aagaagtatg gatctcttag ataaccgaca
    12061 tttttaggat agatatgaat ctcttgcagt tcctcggttg attctgtcgg gtgataaatt
    12121 ggcaaaatag cagcaattgg gatagctgtc tgatcgatat caacatccaa tccaacttct
    12181 ggatcaatcg attcttcgaa aatcaaataa actgcatccc gatcttggtt tttcttaggt
    12241 gctttgatac gtgcaatccc gtctcctaat ccgccaaccg tatctttccc agttgttacc
    12301 gaagtcccta tttggacaag cctttcttct gtgattagct gacggatatt gtttctgttc
    12361 gtttcagcga tttgctggac gaattcttta ggagtcatgt tttcagcagc ttttttttca
    12421 tgataataat cgatcatgtc gtaaatggca aatgtcgctc catttagtgg caccgtctgt
    12481 gaaatgatat ctgctgtttc ccctgcttcc tcagagatag ccaagccatc attttcatgg
    12541 tattcaaatt gatcgttata atcgatatct cgaatcatcc gtttatgcaa cacgaattcc
    12601 acgacatctt cccctgattc ttctgccgac atcgcatgtg gaagaaggac tgctgcaagc
    12661 ggtagtaaaa caagtaattg aagcatcaaa cacaacagct ttttcattac cttgctccct
    12721 cctttttaga ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc atgaagaatg
    12781 cagcaagcag gatatagaat aatcgtccgg atcctcctgt agcaggcaac ggattttcag
    12841 gatcgttccc gacactaagc tgtaatctga ttcgatttgt ctccaaaagt tcatattcta
    12901 actcttgctg tagatcaggc gtctctgctt cttcgtttgg ataatacgaa atctcaactt
    12961 ttagtgtacc cgcttctttt cctggaacta cccgatcatt ttctatgtca ggttcttctc
    13021 gataactaat ttctaaaata aagtaacctg gaagttttct atagctgtca ggtgacttca
    13081 cttccttgat ccgatagacc ccttccaaca agtctgaagc attgaatagg gaagaatcgt
    13141 ttggatctgg tgccaagcct ccctcgatga tttcatactc tcctgcacta ttttgtttct
    13201 caattgaaaa ttctgcgcct tcaagacgtc ggctattcat actgtcaatt ttcaaaagtt
    13261 ccaaatcgat tggcgctagt tcatcttcta aggttacttg tagtacacca tcgatgattt
    13321 catagccttc cggcaaggga gcagtttcgt cttcgatgac cgtatcttca atgataaact
    13381 taccttcttc attcagtgca aactggaaca cttgatcaga tagccgatac ccatttggtg
    13441 cgacagattc cgagacctca tattcaccaa tagacagatg cagaggttgg tcgtaattta
    13501 aatcgacata cttttctact cctacggcac gagaatagaa gtatccgatc ccaggcaaac
    13561 gtttcgattc ttctggttct aacggattac cagtgtatgc tcgatacagc gggaaggaag
    13621 aatcccccac tttcctgata tcgaaacctg caccatctaa tgtatggcct tcatggtcta
    13681 attttctaac tcggatctcc cgataatcaa acgtattgta agcagtgaat gctacccata
    13741 aacgatcctc ttcatctgtt gtatacgtga gatattgcca attttcttca cctgtttgct
    13801 tgagttggat agtccctata gcattatcgt aaagtcgcaa gatccatgtg tcattttcat
    13861 caggttcttt gaaaccttct gggttctggc tctcaatgat tttgtattct gcatagttgg
    13921 tagtttcttc actatcctta taaacaaatg aatctggctc tccgattgga tgaataactt
    13981 tctcaccatc cacgattaca tatttcccgt cttcatctac tgaatagaac gtgagtctcc
    14041 ctttctcttc ctctacttcg ttcacaccag aaagcccttt cgctactgga tatccgattt
    14101 ccagtccgtt accatctgtt tcataaagtt caaactctgc gccattgatt ggcatattac
    14161 tgccttcaat tagtttattg atattcatgt ggattggctt gaattcattt cgcatttcca
    14221 gtttgacata caactcatca tactctgtgc cttctatgtt aggctcttgt acaacagaaa
    14281 gcttgtgata ttctccttca taataggaat tcggatcttt accttccggt ccgccgactc
    14341 tcgaaccaga tgtatattca tctacaagct cgtagtgacc taacatctca tcttcgttgg
    14401 ctgcatattt tttgatttgg aattgatacg tctcatctac taactgataa cgtggtggtc
    14461 cagcgatctc tctgatccaa aatatgtcgc tgactgctcc agtttcatct cctaaaatcg
    14521 gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt tttacttgtt tcgattggtt
    14581 cggcttcccc actctctgga ttttggtcat ataatgcaaa ttcgactcca gccaacggct
    14641 cgccattttg acccttcttg ctgacttgga aattacggcc tttatgttcg taacgaatgg
    14701 aagaagatgg gaaatacatc cgtccagtag tgatatcatt ctcataatcc tccaggttct
    14761 gaatataggc catattgttc actgtgcaaa aatttccgtc ttgagctccc gcatcaagtt
    14821 ttacttggta cttgatcctg accatttccc cttcacctaa agaaagtaga agagatatcc
    14881 tccaaggctg taacaatgtc attcttgttg ttcgcattgt aatatcgcgg tgttacgcca
    14941 tcttcttcta aatcagcaat atttttcaat acatttcgtc caatagcatc tagtcgttgt
    15001 ctcccgctaa cgtttccatc gattcctaaa ccaatggaga agatacttgg cgggttctct
    15061 tgatacttgt ttttcagcca atgatgataa gcaactgtgt tgatcgcaga tgacttttct
    15121 tgcgaagtag gactgccatt cgtattgtta ttagaagaac catcaccaac ccagtgagtc
    15181 gctttattat ctccttcttt tacctctcca taacgatagt gtacatttac ttcatcttct
    15241 ggacggttaa agtcagatgc ataggtaact ggatgagtaa accctgcatt agaagtattc
    15301 ccagaaaact cctcaaaatt tctgaacaaa acgtcattat cttcagcaat cttatcttcc
    15361 atcacagtcc agttgctcca agctccatta gtgctcgtac gataacttga ctgaatcggt
    15421 gcataactga aggtcggtgt cccatctccc acaacgatta ggattttttc tggatttcta
    15481 ttttctccgc cattgtcttt atacaatgtt tcatagccat ttttaagccc tagtgtaagc
    15541 ggagttcctc cgattggtgt acgatttaat gctgtattct cacttcccgt caaacgattg
    15601 atatcatttg tcaatggaaa gatgtccgaa ctgatttgct catggttatt gatagctgta
    15661 ctattatggt aaaaattcac cataccgatt cgaatatcca gactcgggtc agagagcaga
    15721 tctttactca tttcgtttac tgcctcaatc aacgcagcat ttttagtttg actattggtc
    15781 ccagcggtta attcactcat actcgctgac ttatctagaa caaaaacgat atcgattgga
    15841 ctgatttcct ttttctcgcc cccgatgaca tctagataga cattgtaaag ggttgttgca
    15901 tcttcaggat gtagatcaga tgctgtgctg cttggcagaa caggttctac ccattttttg
    15961 atgtacgccc cgttataatc caagtaactg ttttcaaaat tccgttcatt tccccaaagc
    16021 tgaacggttt gagctggaac gtttctatct tcttcgcctg cagttgatcc attattagta
    16081 gcatagtcgt aatttcgcca gttcgctccc ccgtttgtat aatcactatc atcgatcaag
    16141 ttctctccgt catatggcga gcctttgtct ccttgatagc gttcattgct ttgtgtccaa
    16201 tttcctaaca cttttgtata gtcattcgtt gtaaacttca tctattcttt catcaaccac
    16261 cacttgaaga ggtagttacc cttcgttcac atcggggatc ttttctgttt caaaaatcaa
    16321 tgtgccggtt tctgcttctt ttgaataaac ttttccgaca taccattctt gaccatcgat
    16381 cgtctcgact tttaggtctt ctccatcttc atcataggag gatagacctg ttccacgaac
    16441 gttttttacg gtaccaatcc cggcggctgc cgcatcgatt gctaaacgta ttctaccttc
    16501 attggcactt tccttttttt caaaagccag atgccattca agggtttctt ctttttcaat
    16561 atacttccct tcaatttttg catagtcctc atctaagaat acctcatcct cctttgtttc
    16621 tgttgactca gcagctacat ccagggtcgt catatgatag actgactgga aatatggtcc
    16681 aaatagcaaa ctgacagtta ctaactgcaa aagcttcaat tttaaccctt tcacatcctt
    16741 atactccttt caaaaatatt tgtcttcttt cacaacaatc aac
//
LOCUS       DAJSMI010000052        16780 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJSMI010000052 DAJSMI010000000
VERSION     DAJSMI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.052"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(153..1658)
                     /locus_tag="ON430_001328"
     CDS             complement(153..1658)
                     /locus_tag="ON430_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3219253.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1655..2341)
                     /locus_tag="ON430_001329"
     CDS             complement(1655..2341)
                     /locus_tag="ON430_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3219254.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="ON430_001330"
     CDS             complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="ON430_001330"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR3219255.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4125..4304)
                     /gene="rpmF"
                     /locus_tag="ON430_001331"
     CDS             complement(4125..4304)
                     /gene="rpmF"
                     /locus_tag="ON430_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR3219256.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4362..4931)
                     /locus_tag="ON430_001332"
     CDS             complement(4362..4931)
                     /locus_tag="ON430_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCR3219257.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5117..6019
                     /locus_tag="ON430_001333"
     CDS             5117..6019
                     /locus_tag="ON430_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3219258.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6054..7838)
                     /gene="pyk"
                     /locus_tag="ON430_001334"
     CDS             complement(6054..7838)
                     /gene="pyk"
                     /locus_tag="ON430_001334"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR3219259.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(7912..8874)
                     /gene="pfkA"
                     /locus_tag="ON430_001335"
     CDS             complement(7912..8874)
                     /gene="pfkA"
                     /locus_tag="ON430_001335"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR3219260.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9042..12356)
                     /gene="dnaE"
                     /locus_tag="ON430_001336"
     CDS             complement(9042..12356)
                     /gene="dnaE"
                     /locus_tag="ON430_001336"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR3219261.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12587..12772
                     /locus_tag="ON430_001337"
     CDS             12587..12772
                     /locus_tag="ON430_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCR3219262.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12834..14030)
                     /locus_tag="ON430_001338"
     CDS             complement(12834..14030)
                     /locus_tag="ON430_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3219263.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTDALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14200..15636)
                     /locus_tag="ON430_001339"
     CDS             complement(14200..15636)
                     /locus_tag="ON430_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR3219264.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            15929..16738
                     /locus_tag="ON430_001340"
     CDS             15929..16738
                     /locus_tag="ON430_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3219265.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4915 a         3527 c         2944 g         5394 t
ORIGIN      
        1 catctaaaag atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt
       61 tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt
      121 ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga
      181 ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta
      241 tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta
      301 gctcttgctt tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt
      361 attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag
      421 atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt
      481 aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct
      541 aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact
      601 tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag
      661 tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat
      721 gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct
      781 tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt
      841 tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc
      901 aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata
      961 gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa
     1021 cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc
     1081 ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt
     1141 tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt
     1201 gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt
     1261 ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc
     1321 tcagctataa aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg
     1381 acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt
     1441 tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt
     1501 ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta
     1561 aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct
     1621 ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc
     1681 cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac
     1741 gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta
     1801 gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt
     1861 attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt
     1921 cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa
     1981 tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt
     2041 tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc
     2101 ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta
     2161 aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg
     2221 ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca
     2281 actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca
     2341 tttattattt tatcctttct attctactgg ctttttaaaa actctatcat tataccgata
     2401 atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag
     2461 tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac
     2521 agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga
     2581 cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca
     2641 gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta
     2701 cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga
     2761 tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat
     2821 ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga
     2881 tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc
     2941 cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt
     3001 cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta
     3061 cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa
     3121 gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag
     3181 ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca
     3241 gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa
     3301 agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt
     3361 cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc
     3421 cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa
     3481 tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg
     3541 aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc
     3601 cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt
     3661 caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg
     3721 ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt
     3781 cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg
     3841 ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg
     3901 ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa
     3961 ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat
     4021 tgcaaactaa agaacaacat attttttatt taccgactac aaaaaaataa acctcaaggc
     4081 tccatggtta gccttgaggt tttacatttt ctaattaaaa tgtattaagc ttcttttgac
     4141 attacgtcct taccatcata gtaaccacaa tttgcacata cgtgatggct ttttttcatt
     4201 tcaccacagt ttggacattc gtttaaccct ttgattgtca atttgtagtg tgtacgacgt
     4261 tttgcttttt tagcttttga tgttcttcta gctggtactg ccattttgct acacctcctt
     4321 gtaaagtctt gtatctaaat tagacacaat atattccaat cttacttgtt atcgtcgtct
     4381 tttgatttag aatcaaaaag ttcagatagt tttgcaagac gaggatcaat tttattctcg
     4441 gcttcttcca tttttctgcg ctggtattct tcttcggaaa caacttccca atcattgcct
     4501 tttggaagtt ctgttgctgc ttcctcttct tttgtcaata cccgcattgg aatcgccaat
     4561 aagatgttgt cttcaatcga ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc
     4621 tcatctgcag acatttgttc atctctactt tggaattgtt cttctgtcat gaatacttcg
     4681 tctacagaaa aatccatggg cagttcaaca ggttctagtg aacgtgttga aggaactgtc
     4741 aaagtcgtct gcacagtgta atgaacgata tagcctttct tatcaactga gaccaaacct
     4801 tcgacttcca ctggcgcaac atcaagtatt aaattgtctc gcttcatgag agcggctttc
     4861 aagtcgagcg tctcatgaaa agttaacggt gtctcctggt attttctcaa ttccaataaa
     4921 gaccatttca tttttcatca ctcctaagca acaaaagcaa ttatacaggg caaaaagcat
     4981 gttgtcaatg aaattttctt gacaccttgc agttcataaa aaaacagaaa aatctttatt
     5041 agaaagcctc tgtttgctat catcctcaga atcatttaca ataatcatta ttccattaaa
     5101 ggagcggatg atcttcatga acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca
     5161 aaaaaatttt acgaaagtgg ctaattattt ccgtgtgagc caacctacta ttacatatgc
     5221 attacaacgt cttgaaaaag agtttgacac cgtacttatt acaagagatc gatcgcatca
     5281 cgaattgatc gtcacgcctt ctggaaagca gctgtttctc catgcacaaa ccattctcca
     5341 agagatttct actgcaagag ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg
     5401 catgcctcct attatcggca atgaatactt tcctaaactg tcaagttact ttatcaagca
     5461 tgaattgatg aatcaaattg agattaccga tggaggctct cgggacttat acgggatgat
     5521 ccgacaaggg aggatcgatt tagctgtatt aggctcaaca cagccaatcg tggatcaata
     5581 tttagaaaca gagttacttt tggacaaacg tttcatgatc gtcgtgccac ctacacatcc
     5641 tctttccaag caggatactg tctctttcaa agatatagca gatgaacagt ttgtactatt
     5701 gaacgaacat tatgtccacc ccgcagcatt cactaagctt tctaaacaaa cacatactca
     5761 tccaaaagtt gtttatcaaa gcaatgactt gacgatttta aagagtatgg tccgagaaaa
     5821 tgtgggtatc ggttttttgg cagagattgc tgtccatcct gaagatcatt tagtttgcct
     5881 ccctctttca gatgaagttc agcctcgctt cttgatttca ttggcaaaac gaacacagca
     5941 gatcgtcacg cctcttcaag aacaagtagt ccaagttatc catgaattcg ttacagcaca
     6001 aaaaaacagc tcttcgtaaa agaagagctg tttttatttt ttattaagct aaattagata
     6061 gctgttgttt cgccacggta aacgatgccg cgacgtgaat caacggtaat caattcacct
     6121 tctgtaattg ttgttgttgc gcctttagca ccaacgatta caggaatatc ttgtgcaatt
     6181 cctacaactg cagcgtgaga agtcaatccg ccttcttcaa cgataacagc agctgctttt
     6241 tcgattgcag gcatatattc tttgtcagtt gatggtacaa ctagaaccat tccgtctttt
     6301 gcttttttta cagcttcttc agcagatgaa gcaataactg catttgcaac gacagatttt
     6361 tgtccaacgc cttgagcttc taataattta gaaccaatca tttgaatttt catcacgtta
     6421 gttgttccgc gttcaccaac tggtacacca gcagtgatca agattaaatc gccttctgat
     6481 gcgaaaccta attcaacagc ttttttagct gctagttcaa acatttcatc agttgttgat
     6541 ggtttttcag caactgttgg gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt
     6601 tcgtcaaaag taacagccaa gatatctgca tctggacgat attttgaaat catttttgca
     6661 gtgtatcctg attcagttgc agcaacgatt gttttcacgc ctagattttt tgcagcacga
     6721 gctacagata aaccgattgt ttcagttaca tctgttttat cgaattcgtt caattggaat
     6781 gtacctaatt ctgataatgc attttcagct tcttggtcga tacgtgccat tgttgaaaca
     6841 gcttcaactg ggtagtcccc gtttgcagat tcaccagata gcattgttgc atctgtgcca
     6901 tcaaatacag cgttagcaac gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc
     6961 attgattcta acatttgtgt agcagtgata actgggatac cagcagcatt acattttttg
     7021 ataatgcgtt tttgaaccat tggtactagt tcagctggga tttcaacacc catatctccg
     7081 cgggcaatca taacaccgtc agaaactttg atgatttcgt caatgttgtc gataccttct
     7141 tgagattcga ttttagggaa gatttgaaca tgagtcatgt ctttttcttc taagatttcg
     7201 cggatatcta gaacgtcttg tgctttacgt acaaaacttg cagcgataaa gtcgatatcg
     7261 ttgtctaaac caaaacgaat atcgtcagca tcttttggag taatacctgg caaactaata
     7321 gaaacgcctg gtgcatttac ccctttacgt gatcctagca tacctgcatt tttaacttct
     7381 acgactaatt cacggtttgc ttcgtctttt tcaatgattt gcatatcgat caaaccatca
     7441 tcaaataaaa tgtgtccgcc aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg
     7501 attttttctt ttgaacctac atgttcagga tccatagaga tgcgtgtttg atctcctact
     7561 tcaaatttga tgtatcctgc acgaccaaaa tcagcttctg ttgtgccttg aacagttgta
     7621 cggatttcag ctccttttgt atctaaaaga ataccgacat cttttcctgt ctttttaact
     7681 gcttcacgga ccatagtcat acgtgataat tgttcttcat ggtcaccatg agaaaaatta
     7741 aagcggaaga catttgcccc agattcaatc aattgagaaa taatttctac tgagttactt
     7801 gctggtccta atgtactaac gattttggtt tttttcattt aaaaaactct cccattcaat
     7861 tttgtttatt taaagctgta cacagcttaa atagcttggt ttctttcact cttaaaatga
     7921 aagtgaatta tttagatcat atagtgataa gtctggttta tgtttgttgt tttcaagtgt
     7981 atcaatgatg tcagacgcaa cgatttcgtt gtcatggata ccgacacata atccaccttt
     8041 tccttcttga agcaattcga cagcgtaagc accgaattta cttgccaaca cgcggtcgcg
     8101 tgcgcttggt gctccaccac ggacaacgtg tcccaagata gatacacgtg tatgatagtc
     8161 accaaattct gaaagtttct cagcgaattc atttccgccc attacacctt cagccaagat
     8221 gatcaagcaa tgtttcttcc cgcgatcgcg gccttcttgg atttttttag caactgaagc
     8281 catatcaaaa tcatgttctg ggatgatgat ttcatccgct ccgccagcca cacctgacca
     8341 taaagcgatg tctcctgcat tgcgtcccat tacttcgata acgaatgtac gaacatgaga
     8401 agtcgctgtg tcacgaatac ggtcaattga ttctagtact gtattgattg ctgtatcaaa
     8461 accgattgtg aaatcagtcc caggaatatc attatcgatt gttccaggaa tacctactgc
     8521 tgggaaaccg cgttttgtca aagccattgc gccatgataa gagccgtctc ctccgataac
     8581 cactagacct tcgattccga attttttcaa ttgttcgatt ccttttaatt gaccttcttc
     8641 agttgcaaat tctggataac gtgcagaata caagaatgta ccaccacgtt ggatcttgtc
     8701 acctacatct gccacatcaa gacgacgaat atctccagca actaaaccgg caaaaccata
     8761 attgatcccg tatacttcca gtccttcgta aataccttta cgaacaacgg cacgaacagc
     8821 tgcattcatt ccaggtgcgt ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa
     8881 cattcacctc attaaaatac tttatcctaa gtatgactta ggtttacaac catttttgcg
     8941 ttaaccacga ctaacattct aacattattt ttctcacttg tcttgattta cggataaaaa
     9001 aatgaaaaaa aatgaaagca agtagtattt tttaccgtaa gttatttgaa aaccacatta
     9061 tcttcgccaa gcagataaat aagttgccgc tttatactct tgctgtcatc cacccaataa
     9121 gcatctgaca gaactacttt tcgttgcgtt ttttcgtgat acataactac tggtatatat
     9181 cctgggtgtt tctgaaaaat cacttgtaat tgtttcatta tctcgggatc ttcccgttct
     9241 tctgtaatac gcaaatagca agtttgtgaa ggatactggg cttctaactg gttaggatca
     9301 gcaaagcttt ctccaattag ttgcagttcc tgattatatt tgctgatttc gctccgccct
     9361 tgaacaaaga tcactcgatc aacggcaaac aaattacgat atttacgata aagagttgga
     9421 aaaactgtga cagaaatttc tccactactg tcggttcctt caacaaaggc catcagttct
     9481 cctttttttg tccgaatttc tctaacttcc ttgatcatca ctaataaatt gactggttga
     9541 ttagggacga tatctgtaat cagctgaact tgtttagcta aacgaagacg atcaaaccct
     9601 tctgcaggat gacctgacaa ataaatacct aagtactcct cttccagctt caagcgttct
     9661 tctagtgtat agtcagcgac ttcttcttct tttaatgcca tgattcccag caagtcaaga
     9721 cttccaccac tgtaaacgat attttggatt tttccttcga gatcaagcat caattgtttc
     9781 ctgttaggag ataattcatc aaaaactcct actgctgtca gtgattgcag taactctaat
     9841 ttcagccaac gcttatcgat ccgtatgagg aattgatcca ctgactggaa cgggccattt
     9901 tgttttcttt cgtttaatat atcttctaca aaatcacgcc gaataccttt tatcgctgtc
     9961 agtccgaatc tgatttcttc cttattgacc aactcaaaac tggaattact ttgattaatg
    10021 gacggcgagg aaagtttgag tttattttta cgtgcttctg caatatattc tttcaatttt
    10081 gatgtgttat gccgaacaga attcatcagg ctggtaaaga aagcacctgg atgatgggct
    10141 tttagatacg ccatttggaa tcccacgaat gaataagcaa atgcatgtga ccgattgaat
    10201 ccatagtttg caaatttttc gatatattca tatacggtcg ctgctgtttt ttccgaatat
    10261 ccctgttgta atgctcctga tacaaaatgc gacttttctt catctaatac gtcttttttc
    10321 tttttactca ctgcccgtcg cagaatatct gactgcccta atgtaaatcc agccatagtc
    10381 gaggcgatct gcatgatttg ttcctgatag acgatcactc catatgtgtt tttcaaaatc
    10441 gggatcagtg aatcatctgg gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag
    10501 gtatctatat tttgcatagg gcctggtcga tacaatgcat tgactgcggc aatatcttcg
    10561 atgttttctg gatctaatct tcgtaaaacg ttccggatac cagcagattc aaattgaaat
    10621 actcctgagg tttcgcctcg ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc
    10681 tcttttaatc ggatctcata cccttctact cgcttaacag ctttcaaagt aaaatcaata
    10741 atcgataagt tgcgtagtcc taaaaagtcc attttcaaaa gaccgattgc ttctacctca
    10801 ttcatcgtat actgggtcag aaaagcttca tcagaaccgc gttggagcgg aactagttca
    10861 agtagattct tatcgctgat aactacacct gctgcgtgag tcgaaacatg tcttggcaat
    10921 ccttctaaaa tcactgcagt tttgaataaa agttggtttt tttcgcttgc ctgaactaat
    10981 tctactaatt gatgcgaagt tttataagct tgggtcaatg tgattttcaa ttgattgggt
    11041 attgcttttg accagcggtt tgcttcactt tgcgataaac caaacactct agctgcatct
    11101 ctaagaacca tttttgctgc cattgtgcca aatgttgcga tttgtgccat atgatattgt
    11161 ccatactttt ctcgaacata attcaatact tcttcacgtc gattgtcagg aatatccaaa
    11221 tcgatatctg gcatcgtaaa gcgttcgggg tttaaaaagc gttcgaacag caaatcatat
    11281 tctaatggat ccacatctgt gatttctaac acataggcta ctaatgaacc cgcagcagaa
    11341 ccacgacctg caccggtaac gattttgttt ctatgagcaa aagccattac atcccatacg
    11401 atcaagaaat aatcatcgaa tcccatttga tggatgacag ataactcgta gtccaaacgt
    11461 tcttggtatt cctttgtata gtcagtgatt cggcgttcca cttgcgaata gcataactct
    11521 cttaaatatt ctccagcttg ttttccatta ggaactggat aatgcggaag taatttctga
    11581 tggaactgga attcaaatga aatcgattca gctaaacgct gggcgcctat tacagcttgg
    11641 gaaagacccg ctttttcgaa tcgtttgatc atttcttgtt ctgaaagaag atagacattt
    11701 gctgttagtg gtaaagatgc agataaattt tctaaaggta tttggcttcc ctctttgata
    11761 tgtttcatca cttcaagtgc aaaaagttct tcttgttgta aactagcgat gggatgtaat
    11821 gcacaaagcg aataattttc ttttatcaca tactgatacc attcaggcaa ttgatcaggc
    11881 gtgttttctg tcaagccgat ataaaaattt ctagtaaaaa cattttcaag taaaaactgc
    11941 caatgaccga aaaagttatc ctctttattg aaagcctcag aaacagaatg atttgccgga
    12001 acaacaaacg ctagatggtc ggtttctgtc atttctgcaa gttccaattt ttcttggatc
    12061 atttttcggc tggaaagttt catcagctgc tgataaccta tttcatcttt tgcgtacgca
    12121 agcaagccat aatttttttc ttcataagta taactaagat gtaatccaat gattggctgt
    12181 actccttctt ctacacaaat acgatagaat tcaggtgcac cgtataagac atctttatcc
    12241 gttatggcta atgaagtgta gccttgtttt tttgcttctt gtacatattc tcgaatacgt
    12301 atcgtacttt gtaaaagaga ataagaagta attgtataaa gttgtgggaa aaacatcttg
    12361 cattccgcct tttttttcgt taaaattcac tagcgttaaa aggtttgatc cttttacttt
    12421 cattaagata gcaggcgctg aagttgatta ttccacttca tttgcttgct tgtaatctgt
    12481 aggaatgagt tctttcgcaa gaactcaaat tttattgaga tttggtctcg tttctttaca
    12541 ttcaaagaaa atctggtaaa attaagatag aaaagaggtg ttctttatgc gatatatcgt
    12601 tacattattc tgggctgtcg ttctaggtca agtagtcggt tatattggtg ctgcattaac
    12661 aagcggaaca tatgatttta ctttgacaac aatcatttca ttcattgcgg gtgtcattat
    12721 ccttttgatt ggtgcagttg cgccaagaaa agaaacctct gcccattcgt aaaaaaacag
    12781 ccaagagctt gagatttctc aagctctttt ttattttcta ctcctcacca ccattataat
    12841 ctgctgcgaa gaactttacg aaagttgaat aaagaaagaa gaatatttga cagaacaaac
    12901 aaacttgtca caataaaaac agcactgtaa ttataagcat gcgcgactgt cgatcctagc
    12961 atgggaccta aaacttgtcc aaagttgcta aacatctgat tgtagctata gattcggctg
    13021 actccttctt gaggcgtaat cttactgatc agtgtattga cagaaggcat caaagcgtca
    13081 gttgaaaagc ctaaaataaa gcgaagtata cctaattgga gcggtgtagt gacgaatgcc
    13141 ataggtaaat aacagcaaaa tgataaaatg agtcctccaa ttagaacttt ttgattccca
    13201 atcttatccc ctagttttcc tagtttcggt gaagaaaaga cagcggaaac accagctatc
    13261 gaaacaatca agccgctgac aaaaaggata ttttctgtac ttccgctcaa ttcacgaata
    13321 tataaggtca aaatagggct gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg
    13381 aagagaccga tcaaaataga gagatgatcc atttttgaaa aaatttcttt ggtgcttatc
    13441 agatcttttt tctcgattgg atggaaatct tctttgacta agaaaatggt caataccgtt
    13501 gtgatcatca acaaagcccc tgtgatcaag aatacatttt ccataccaaa ccactgagcc
    13561 agtgcaccgc ccattgaagg tccgatcaga tttccagcaa tcgctccggt agacaatgtt
    13621 cctaacgccc atccgctctt ttctctaggt gcctgtgaag caatcatcgc tgtggcattt
    13681 ggaatatatc cagacaaaat cccattccaa aaccgcatga tcaaaagcca atacgcgttt
    13741 ggtacaaaag ccaaagatcc cattgttaat gtcatccctg ctgctgcacg gatcatcatg
    13801 agttttcggc cttttcgatc agctagattt ccccagattg gtgcaaccac ggcagatgca
    13861 aaagcagtca cagagattgc tagtcccgaa aacaattcga tttgatcttt tggtgttcct
    13921 agttgctcaa cataaactgg tatgaatggc ataactaaac taatacttgc accagtgaaa
    13981 aaacagccaa tccacgcaat cactaaattt tttctccaat taatctccat ctatcatccc
    14041 tctttcgtct cttttatcat acctattttc gaataaacaa gcaacgatct cgccagtaaa
    14101 aagagaaaaa tcccgcaaag gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa
    14161 gagatcagaa caaatactgt tccaatccct tctctttagt taaattgttg ctagaatgat
    14221 gaagttgata atgaataaaa tatcaacgat ccacaaagca acagaaactt gattgtattt
    14281 tcctttgatt actttcacaa ttgtatagaa aataaatcct gctgcaatcc cataggaaat
    14341 gctatagcac aatcccataa agatcgatgc aaagaaggcc ggtaaagctt cttccatatc
    14401 tagccatttg atgtctgcaa aagaagccat catcattact ccaactaaaa tcaatgctgg
    14461 cgcagttgct tgatttggta caatggcgat cagtggagag aataaactac ttaatgcgaa
    14521 tagcactgcc actactactg aagtcaaacc agtacgtcca ccggccccga tcccagctgc
    14581 agattcgaca taagttgttg tattcgatgt accaaagata gcccccacag aagtagcgat
    14641 cgcatcggca aatagtgctt tatccatttt tgttttgaac ccacgaccat cttccaacgc
    14701 gatctcgtct tcttttgaga aaatacctgt gcggcgtcct gtaccgataa atgttccgat
    14761 cgtatcgaaa gtatcagata aactgaatgc gatgatcgtc atcaatactt gaggaatctt
    14821 ggaagaatca ctgaataaag attgcatgcc ttcagaacca aatgctgcac cgaaagttgt
    14881 tcctaattct ttaaatgaat tacctaacga attcgtttgc caatcaatgg aacttaaatc
    14941 aacaacaccc ataaaaatac caaggactgt ggtcgctacg ataccgataa gaatagcccc
    15001 acggacattt tttacaacta aaatggctgt cagtaccaaa ccaatcacag ccaataagat
    15061 aggagcgttg tcaaagttgg ctaacgcagg gacgattcca ccatttatcg taacatttgt
    15121 cgcttttccg ccttcaacga cagagctcga aatagctgac tggtctgctg aaaaagataa
    15181 gaatcctgca tttttgattc cgacatacgc aacgaaaata ccgatcccgc caccaatcgc
    15241 atgttgaaga ctttcgggaa tcgcccgaat aatcattttt cgaatatttg tgacagtaat
    15301 aaaaatattg attaatccgc aaataaacac cattgccaag gcttgttgcc aagaataacc
    15361 aagcccaaaa acgaccgtaa aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc
    15421 atatggtaca tttgcgaaca atcccattac cagcgtacca ataatcgatg caatgattgt
    15481 cgctagaaat actgcttgga agggcatacc tgatgctgac aaaatcgtcg gatttacgaa
    15541 taagatataa ctcatggcaa agaatgtcgt tactcccgcc attatttcag ttgatacatt
    15601 tgtattgttt ttctttaaac cgaaaaactt ttccataaaa agttccactc cctgaatttt
    15661 tttctgatgg aaaactataa taataaaaaa taagtaaaat agggcaaatc catcgaacaa
    15721 tttcaaatta tatagacgtt tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa
    15781 ataatactta acgttcgtgt ttagcttatg tttagctatt tatatagcca taactgtagt
    15841 ttttgttagg aatagctgat tgctcttgtt aattacctaa aatatgctag actaaaacaa
    15901 atgctagttg aggaaggaag gacccttatt gaataaaaaa ttaattgcca tcgatctgga
    15961 cggaactacc ctcaatcaag attcgatgat tacatctaaa acaaaagaga ctctgaaaaa
    16021 agcaatcaat gccggtcatc atgtcagtat tgctacagga cgcccattcc gaatgagcca
    16081 tcagttttac caacaattag aattaacaac accgatggtt aattttaacg gcgcattagt
    16141 acacatccct aatcagcact gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc
    16201 ttttgaaatc ttatcgcaaa aaaagcagct aaatttagat tttatcgctg ctgaaaatcg
    16261 tgatactttt tttattgaca gttttgattt ctttgatgaa aaaatttttg cttcttctcg
    16321 tccaggagag aagaatttgc tttctcctaa aaatttgacg acaaatccta catctttgct
    16381 tgtccgaaca gacaaacgtt ttgcaggagc tgtatctgct gaattaacac gtcaatttgg
    16441 cagttatgtc gatgttcgga catggggtgg acctactgcc atcttagaga ttgtttcaaa
    16501 aggaatccaa aaagcaaaag gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa
    16561 agacgtcatt gcctttggtg acgagcataa tgatttggaa ttgttggatt atgcaggttg
    16621 gggagtagct atggctaacg gtactgacca actcaaaggc attgcaaatg atgtcactcc
    16681 tttgagcaat caggaagacg gtttagctgt ttatctggaa aaactactta aactataata
    16741 gagaattaga ttccacaaaa aagaggttgt gacaattttt
//
LOCUS       DAJSMI010000053        16362 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJSMI010000053 DAJSMI010000000
VERSION     DAJSMI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.053"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="ON430_001341"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="ON430_001341"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCR3219266.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="ON430_001342"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="ON430_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCR3219267.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="ON430_001343"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="ON430_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCR3219268.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="ON430_001344"
     CDS             2857..3732
                     /locus_tag="ON430_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCR3219269.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ON430_001345"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ON430_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR3219270.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="ON430_001346"
     CDS             4629..5063
                     /locus_tag="ON430_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR3219271.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="ON430_001347"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="ON430_001347"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR3219272.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="ON430_001348"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="ON430_001348"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR3219273.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="ON430_001349"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="ON430_001349"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR3219274.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="ON430_001350"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="ON430_001350"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCR3219275.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="ON430_001351"
     CDS             13334..14260
                     /locus_tag="ON430_001351"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCR3219276.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="ON430_001352"
     CDS             14442..15335
                     /locus_tag="ON430_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCR3219277.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="ON430_001353"
     CDS             15469..15594
                     /locus_tag="ON430_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3219278.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="ON430_001354"
     CDS             complement(15728..16360)
                     /locus_tag="ON430_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219279.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5811 a         2788 c         3492 g         4271 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aa
//
LOCUS       DAJSMI010000054        16295 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJSMI010000054 DAJSMI010000000
VERSION     DAJSMI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.054"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(36..899)
                     /locus_tag="ON430_001355"
     CDS             complement(36..899)
                     /locus_tag="ON430_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3219280.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1095..3194)
                     /locus_tag="ON430_001356"
     CDS             complement(1095..3194)
                     /locus_tag="ON430_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR3219281.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3414..4523)
                     /gene="rpoD"
                     /locus_tag="ON430_001357"
     CDS             complement(3414..4523)
                     /gene="rpoD"
                     /locus_tag="ON430_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR3219282.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4547..6388)
                     /locus_tag="ON430_001358"
     CDS             complement(4547..6388)
                     /locus_tag="ON430_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR3219283.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6570..8885)
                     /locus_tag="ON430_001359"
     CDS             complement(6570..8885)
                     /locus_tag="ON430_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3219284.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9070..9930)
                     /locus_tag="ON430_001360"
     CDS             complement(9070..9930)
                     /locus_tag="ON430_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCR3219285.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(9989..10621)
                     /locus_tag="ON430_001361"
     CDS             complement(9989..10621)
                     /locus_tag="ON430_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCR3219286.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            10788..11549
                     /locus_tag="ON430_001362"
     CDS             10788..11549
                     /locus_tag="ON430_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3219287.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11575..14166
                     /gene="mprF"
                     /locus_tag="ON430_001363"
     CDS             11575..14166
                     /gene="mprF"
                     /locus_tag="ON430_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR3219288.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14354..15541)
                     /gene="tuf"
                     /locus_tag="ON430_001364"
     CDS             complement(14354..15541)
                     /gene="tuf"
                     /locus_tag="ON430_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3219289.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            15909..16265
                     /locus_tag="ON430_001365"
     CDS             15909..16265
                     /locus_tag="ON430_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3219290.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4457 a         3379 c         2874 g         5585 t
ORIGIN      
        1 ttataagcta gttaagaaaa acatgcaagg gattctcaat tagatttttt gactagtttt
       61 gtcacaccgt tttcttgtgt aatcaatcct tgtttcaaaa gattacctaa tgcgcgctta
      121 aatgctcctt tgctgatacc aaaagctttc atgatttctt ctggatccga tttatctgta
      181 aagttgattt gctgatctgc agaacgttct aaaatggtta aaatcatttg tgcatcatct
      241 gatatagctt catatgctct tggcttcaat gaaagattca gtacaccgtc tggtcggaca
      301 ccaatcactc gaccttttat ctgctcgcct agacgaggtt cattatatcg ttcagaagga
      361 tggataaacc caagatagaa atcttccgtt aaaacgtacg tacctgttaa tttcaagcgg
      421 tatgctgttc ctatcacatc tttgtttttc atttcttctg ttccatattt gcttaaagat
      481 ttgaatattt tttcatctgc taaactgccc caaatacggt cttttgcatc aactctaagg
      541 gaaatcatca agcgatcccc ttttttaggc catagctctg tcattgtcgg caattcatca
      601 agagatacga caaagtcttt atctggaaga ccgatatcga caaacgcacc taaatctttt
      661 cgtgtagcag taacgattcc aaaagcgtaa tgaccgatac ggcttttagg aatttgtgta
      721 gtgatccgat tttcatgttt ttgattttgg taaccgaatc cttcaactgc ttcaccgatt
      781 ttgtgttcac cttctgattt gcttaagcca aaggtttgtc cgtttttttg gacaaagtag
      841 tttttttcat tttcatcaat aataatacct gtaaatatag ttgctaataa atcgttcatt
      901 tttttctccc tctatagtga aaggttttct taatacattt ctttgctatc ataacataat
      961 gttatctact tgaatagctg agccttggtt tggaaaggtg aaaaaaggag tatgacaaca
     1021 gttgtgtcat actccccttt ttcgaataaa cggtgttaaa cggcaggctc acagcctctt
     1081 tcatttgcct aaaattaatt gctcccattt cctccgaacc atccagtttt acagttgatg
     1141 gttacgatgt aaaatggcaa agcactttct ccaatgcctt tcatcaatgc agattttgtt
     1201 gaagtggcat atagattata atagccttcc ccattttcga ttctggcttt ttctaatctg
     1261 taacctcctt caacgatata tccacgttgg atacatgtag caatcacttt gctttggaca
     1321 cctggtgccc acgtccatgc tggattagat gcatcggcaa tatcacttgc tctggcagat
     1381 ttgattggct gattcgctga gtttgaagag gaaggggtcg aggatgccgc ttcttgctgt
     1441 gctgcttgtg cgaccgcagc ttgttgagcg gcttgagctg cagctgctgc ttgagcggct
     1501 tgttttgctt gttcttcagc tgcttttttc gcttcttctg cttttttctt ttcctcttct
     1561 tcttttttct ctttatcagt gagcatctta tctacttgtt tcaattgttc attcaactga
     1621 gcagatagat ctgtattttt cactttttct actgcttctt ttgccgtctt gtattggtcg
     1681 cgtgttgctg aatccactac ttttccgtct ttaaagacta tagctgtttt ttctttagct
     1741 gtttgcaatt cagcatattg atttttagct tctgtttgtg cttgttgaat caattttcca
     1801 aaatctgtgt tatccgtttc gattgcttgg atctctttat ctgctttcaa cttgatgtca
     1861 tctgctagat gatcatcgac gatggcaggt gctatgaacc actcattcac tttttggata
     1921 tctcggatct tagattggat atcttggtaa gttgtttcta actctgaata tcctttttca
     1981 ttcttaagac tggataaatc attttttagt tttgataaat cttgatgaat catatctgca
     2041 cgaataaatt gatggtgatc atctgtataa aaggcagcaa gtttctcatg aagttcttcc
     2101 atttgttgct gtctttttgc ttctgctgct acttcggctt ctactttttg atgatggtca
     2161 taataagccc agcttcctcc accgagtaga agtactgctg ctaaaacgag ataaggtact
     2221 ttttttgatt ttttcttttg cgctttatct ggatctgttt tttctaagtt tgattcttga
     2281 tgtgtttttt cagtatctgg cacgattgtt attgatttcg cttctgcttc aaacagctta
     2341 ggctggttct cttctttttc tatttttgct tcaagatcag taccctcttt gcttacagat
     2401 tgattttcag catcggattc actatttatt tcatcgggct ctgttgactt tttgtctgtg
     2461 ttttcgagta tttctttttt ctctgcttcc ggcagtttat tcgttttttc cttttctaat
     2521 tgtttttcag taaatacttc agttttttct gtgatatccg tttttacgtc ttttattttt
     2581 actgtttctt tacctgtggc gttttctcta tcagagtctt tttcagtttc ttgttccttt
     2641 tctggctcta ctttgttttc ctgtgcaatt ttttcactga tcattttgag cagctctttc
     2701 gcgtgctctt cttttgtatc ttcacgatgt gctttgatat aagcggatag tatcgaattt
     2761 tcttcctcat tatcgacgat ttctttcagt tcagctactc ttcttttttg ttttgattca
     2821 agcgtttctt cttgattctt tcctgattct tccgaagtct catgaacagt tgctttcttc
     2881 tgttcatttt tctctatatt agttacttga tcgtgagcag gtggttcttc gtcttctggt
     2941 tctgcttttt cgaataattc cattacagat tctagaggta aatctttcaa ttccgaccat
     3001 tcgattggat cgtttaattc tgtatcagga aagactgcat cttcagttga tttatcctgc
     3061 atagctgcat ctgtatttgt ttcttcaatt gattctttta tttgctgatc ttctgcagtt
     3121 atttgatgac cgcagtctgg acagatagta ttctgctcca tttttttcga tccgcaattt
     3181 ggacattttt tcatcactta gcgatcggct cctttactag gtttacgcta ccatttaatc
     3241 tatcatattc tatacgtcac gccaatagct tatttttagg atttacttaa aaataatgaa
     3301 tgaatttcct tcctatagaa aaaaaagctt gagactgcgt agtctcaagc tttttttctt
     3361 ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca cgcttattct
     3421 aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa tgctttagcc
     3481 tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc ttccaatgtt
     3541 ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc ccgatcagtc
     3601 aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc gtgttctgct
     3661 ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc ttcttcaccg
     3721 attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg gactttttct
     3781 gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc aaggtcttgc
     3841 agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg aactggaata
     3901 cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat ccaccatgta
     3961 gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc cttcatcagt
     4021 cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac ataacgttta
     4081 gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc ttcttggtcg
     4141 ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag ggagacacgg
     4201 ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt tggcgcagaa
     4261 agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct gtgaattgat
     4321 ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg gatcaatttg
     4381 tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa atcatcatat
     4441 acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc ttcatacgtt
     4501 tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat ttggcttgtt
     4561 ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc tgtttgtttc
     4621 cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca gctaagccag
     4681 atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct tctgccaccg
     4741 gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa aagtctaaaa
     4801 actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa aaataaactt
     4861 ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta cggacagaaa
     4921 cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc gtcaaaggtc
     4981 tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc tgaggttctt
     5041 gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc gtctctctgc
     5101 cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt ggtgaaacaa
     5161 ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt tcattatcta
     5221 aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg cgcccatgag
     5281 ccactaattc ttggaaagat gcttctccat acttacggag atattcatca ggatcaagct
     5341 tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc aattcaatcc
     5401 ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc aattctttcg
     5461 tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata gaagctaatc
     5521 cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg aacaacaaaa
     5581 tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa acttgtcttt
     5641 tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt tcctgccctt
     5701 tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa ttacgaagtg
     5761 gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc gtgacaaaca
     5821 atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact tgctgaagaa
     5881 aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct aaagacaacc
     5941 cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat agcatgtgat
     6001 gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca gattcaggat
     6061 tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt tcgaagtctg
     6121 ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga aaagtaaaca
     6181 cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct gcaacagaaa
     6241 aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta ccagactttt
     6301 tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt actgtttcga
     6361 taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg atatcttttg
     6421 ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa ctgaaatcag
     6481 tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat tgctttttca
     6541 agtataacac atttctatct cattaagcat caatttgcta ctcttttcag taactgattt
     6601 tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat gaatacaaag
     6661 aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg gaacagcatt
     6721 gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa tgggttgaag
     6781 ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta atacatcaaa
     6841 gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac cgcaatgata
     6901 ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg caaataacgg
     6961 taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata cccttcaatc
     7021 gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc tgtaacggca
     7081 catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc tgagactttc
     7141 gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt agtaaaatca
     7201 gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc tgccatagca
     7261 atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc tgtcattgca
     7321 accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg cggactcact
     7381 cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc agtaatttga
     7441 gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat attggagaca
     7501 gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg aagataagct
     7561 agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc aaccggctca
     7621 acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa cataagtacg
     7681 cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc ttccaccaaa
     7741 cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc gccccatttg
     7801 cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag ctcaaaataa
     7861 tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata cgtccccata
     7921 atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg gatactcatt
     7981 ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg ggccagcgtt
     8041 tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt tacccctgtc
     8101 gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa caaagctgct
     8161 gcagatgcaa taacagaaat tttgatagaa gcatcgcgga tgaacagtgc ttccaaaaat
     8221 agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt gcgaatcgaa
     8281 tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa gcggacagca
     8341 taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt caaaaagctt
     8401 ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc tgtcaaaaga
     8461 gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat ctgttctcct
     8521 gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga ttgttcttta
     8581 ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg tacaagattt
     8641 cttgcatgta tttcttgata aattccaata acgatattga cgacaataat tgctaaatac
     8701 gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa gttcagaaaa
     8761 ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga tgcatcttcc
     8821 tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg tgtaagtcca
     8881 ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg tttaagtcta
     8941 gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa cacacgaatg
     9001 attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt ttttattatc
     9061 aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta gtgctgtacc
     9121 ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg cgtaatcggt
     9181 aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga atagcttgct
     9241 atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac aaagaatccc
     9301 accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt cagccatcgc
     9361 tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga cgatcatacc
     9421 caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga cttgcccgtc
     9481 aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa caaccatgat
     9541 gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg caggaacagt
     9601 tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac ctaataaaaa
     9661 actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa caggcagaaa
     9721 aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa tcagccgctc
     9781 atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga taaatcctga
     9841 accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt accccacttc
     9901 ttgattttcc attttttgcc ttttttccac tatttgcgca ctccttcttt catttaattt
     9961 tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag gtctctatta
    10021 ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta tgatagagat
    10081 agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc ccttcctcat
    10141 ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct actgtgtttt
    10201 ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat gcttggacag
    10261 ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc aaatatttga
    10321 caccttgatc aatactgtca gctggattat caatgatctc agcttctcca tgcagacttt
    10381 ccgagctctg catgagatct gtcccttttc cttttgattc ggtcaatatt atcgccttga
    10441 tcaaattctc gtagttttcc atatgataat tttttgcagc tgttgttatt tctatttgat
    10501 agctttccac tcgttttacc gaacgataag ttcttcctcc taaaaacaac accgcaccaa
    10561 taaccgtcag aatgatcatg taaaaaagaa tacggataat agataatcgt tttcgcttca
    10621 tttcatcacc tagttatttt gacatctttt tttatcttac tatttcttta tcttcttggc
    10681 tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat gaattttcct gtattttttg
    10741 acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg actgatataa
    10801 tcgttatact ttacacagta ggtgattatg tgaaaaaaga aaagaaatat gcaaaaatgc
    10861 cagatgggag cgaaatttac tatgaaaaga gcggtcaagg cttccctctc tttttactgc
    10921 atggcaatga cggcagcggc cgcttttttt cagaacaggt ccctgtactc gaacgctact
    10981 ataccgtgta tctagtagat agccgaggac atggtcgttc gacaaacgaa gcatctatgt
    11041 tgaattttca gttaatggcc gaggatctga atacgatcat gttgctggaa aaaatcgatc
    11101 aggcagattt tcttggattt agtgatggag caaatctagc actggtattt gccagcagct
    11161 tccctaaaaa agttcatcga ttgattttaa attctggaaa cacgcttgtt aaaggcgttc
    11221 gtttttctgc cagagtcatc agtaatattc attatgcatg ggtatggtta ctctctttat
    11281 ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact tttgctgcat gacatcgggc
    11341 ttacagaaaa tgatttaaaa aaaatcaact cccccacttt gatcatcgtg ggaaaaaaag
    11401 atgtaatcaa gctaaaacat tctttgtata tagctaaaac tataccaaaa gcttcctttg
    11461 tgcttgtgaa agaacaagga catgaattag ctagaaaaga tcctgaacgc ttcaatcgag
    11521 aagttcttca atttctttct gaaacataac tattaggaat agaaggtgat ttatttgtta
    11581 aaaaatacca tacaatggct gaaaaaccac ctaggactgt tcaaaacaat ctttttgatt
    11641 tctgtcattg tcattattgt cggtgaattg atgtccatcg gtaaaactct atctatccag
    11701 caattaggag aaacgtttgc cactatacct gtatggaaaa gcggactaat gctattgatt
    11761 ggtcttatct ctgtgttacc tatggttggt tatgatatta ttttgaatcg gatgcttgaa
    11821 caaaagcaga atcctcgtta cctgtttgag acaagctggc tgatcaatac gatcaacaat
    11881 attgccggtt tcggcgggtt tgttagtgtg gggcttcgct ctgaactata tggacaaaag
    11941 aaagagagca gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt gatggctgga
    12001 ctttctattt acagtctgct ttctttttta cttgtagtct tcacgccagt tgctcctttt
    12061 ttgaagcaat attggatatg gttgatcggc ggcggtctat attttcctgt cgtcttttta
    12121 tttactactt tcaaaaagaa aggattcctt ggtggtacct cacttaaaac tcaaggacag
    12181 ctattgctag tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct ttctgttggt
    12241 tatttaatgg aaatccggat tgatctgtgg caaacaattc cattattcat tgctgcttca
    12301 gtgataggaa tcgtgtctat gattccagga gaaattggga gtttcgatgt aatgatgatt
    12361 atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat cttgctctat
    12421 cgcctgttct actacatcat tccgtttttg attggaatcg tctttttctt caaaaatatc
    12481 ggttccactt ttgatcagcg ttattctggt atccctaaac aattagcaac agaaattgcg
    12541 cataaaatcg tggttgtctt attatacttt tctggaatca tgctagtact ttctgccact
    12601 atcccgcaag cttttacaga gtttcgctgg cttcatagtt tgaacccatt gaaatttcat
    12661 tttattatcc aatttcctag tatactattg ggatttcttt taatcattat gggcagaggg
    12721 atcgcagcac gtgtgaaacg agcatatttg cctacaatct tcttgattgt tctagcttta
    12781 ctttatgttt tattaagtga ttttagtttc acaccggtgg ttttcttgtc tattctgctt
    12841 ctgatcattc ttgcttctaa aaacgaatta tttcgggaac aattgatcta ttcatgggag
    12901 tggcgtacga tcgatggaat cattattggt gcactgtcct tgctttacat cgttattggg
    12961 gtatacaatc tgccagattt ccctcatcgg cgtcaccatt ttatttcctt tttcttattt
    13021 ccttctgaaa agatttggtt ttcgggattg ttggccatca tcgctgttag tttcatgatc
    13081 gttctatttg ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc ctttaatgaa
    13141 gaaaaggccc tgaagatcct tacaacttat ggaggaaact ccgatagtca attgatcttc
    13201 ttaaaagata agcggatgtt tgcctatgaa aaagacggtg aaccaactgt tcttctccaa
    13261 ttcgcttgct tcaataataa atgtattgta atgggtgatc cttctggtaa gaaagaagat
    13321 tttccagaag caatcgaagc ttttatcgaa gaaacagatc gactatgcta tttaccagtt
    13381 ttctacgaaa caagcgaaga gattgttatg attttgcatg aattcggcta cgatttcatt
    13441 aagatggggg aagaagctta tgtagacctg aatagtttta caacttcagg gaaaaaaatg
    13501 aagggcactc gtgctgtctt gaatcggatc gaacgagaag gatttacatt tgatgtctta
    13561 caaccaccat tttctactga gcaaatgagt actttcaaaa atatctctga taattggttg
    13621 ggttcacgaa aggaaaaagg gttttcttta ggctttttct ctgaagatta tctacaaaga
    13681 gcgcctattg cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc aacgatcatg
    13741 cctacttaca cgaacaataa agtaggaacg attgatctga tgcgatatga ttctaaaaaa
    13801 gcaccttctg gcagtatgga tttcttgttc ttgcatttgt ttgcctatat gaaagaagaa
    13861 catatccaat ggttcaattt agggatggcc cctctttcaa atgtcggaac ttccagaaag
    13921 agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca tttttattct
    13981 ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc acgttatact
    14041 ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat tattgataat
    14101 gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat acgaagaaag
    14161 tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga gactgaagta
    14221 aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa agagcggtcc
    14281 aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta ccaaaatatt
    14341 cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact gtccgtccgc
    14401 cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc aagtcaacat
    14461 caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat gtgattgttc
    14521 ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag aatggagtat
    14581 gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt gtatgtggag
    14641 taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt tggatccccc
    14701 gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta cggaacattt
    14761 ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc tctacctcat
    14821 caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct cgacctgtaa
    14881 ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt tctggtgttg
    14941 ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca tctggatcgc
    15001 cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct gggaaaccat
    15061 attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct tcatcatcga
    15121 ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg cgtgacaata
    15181 aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact aagattgcac
    15241 catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt cctggtgcat
    15301 caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta ttgatcgtaa
    15361 tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga ggatttgcta
    15421 ggcctttttt gcctaatact gtagtgatcg cagcagttaa agtagttttc ccatggtcaa
    15481 cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt tcttttgcca
    15541 taatttacaa acctcctaaa taatatagat cactgaaaaa tttcagctct caccttttta
    15601 ttatagtctt tttttgacta aatcaaaaag aatacgtccg acttactttt tgtaagattc
    15661 gaactaagct tattatacgt caatattggt caaagtcaac taagagcact cgtctgtctt
    15721 atacgcgaaa aaagtacctt ttataaataa aaaaatgtgt tttttgagaa aaacttcaag
    15781 atagtcctta actttctttt gaagcaataa gaacaaaatg aggaaaagtt taaaattcag
    15841 ctgttgccaa caactttcaa tagacgaaat cttctatttc gttaataata aggaaagagg
    15901 tgaagtacat gaaaaaatta attggcgtac ttgtcgtttt aggcatattt ctcggcggaa
    15961 gttttgcagc ttatcattat ttttacggcg gcgaagctta ttatacaaaa atcacgacga
    16021 atggcgaaat gtcagctacc caagcagata acggagagaa attcacgact tatacctaca
    16081 agcaagcggc ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc gaagaaagag
    16141 aacatccatt aaagatgaat gcgtatctta aactcaaagt caatcctcgt aaaggtgtat
    16201 taagctggga agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa aaaatcgata
    16261 attagtaaat ggtcttaaaa ttttattaga taaaa
//
LOCUS       DAJSMI010000055        16167 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJSMI010000055 DAJSMI010000000
VERSION     DAJSMI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.055"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            221..889
                     /locus_tag="ON430_001366"
     CDS             221..889
                     /locus_tag="ON430_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR3219291.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1089..1475)
                     /locus_tag="ON430_001367"
     CDS             complement(1089..1475)
                     /locus_tag="ON430_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219292.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1447..2829)
                     /locus_tag="ON430_001368"
     CDS             complement(1447..2829)
                     /locus_tag="ON430_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3219293.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2985..4172)
                     /locus_tag="ON430_001369"
     CDS             complement(2985..4172)
                     /locus_tag="ON430_001369"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR3219294.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4179..5210)
                     /locus_tag="ON430_001370"
     CDS             complement(4179..5210)
                     /locus_tag="ON430_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3219295.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5226..6680)
                     /locus_tag="ON430_001371"
     CDS             complement(5226..6680)
                     /locus_tag="ON430_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3219296.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6855..7760
                     /locus_tag="ON430_001372"
     CDS             6855..7760
                     /locus_tag="ON430_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3219297.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7851..8528
                     /locus_tag="ON430_001373"
     CDS             7851..8528
                     /locus_tag="ON430_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3219298.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8525..8695
                     /locus_tag="ON430_001374"
     CDS             8525..8695
                     /locus_tag="ON430_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219299.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8809..9705
                     /locus_tag="ON430_001375"
     CDS             8809..9705
                     /locus_tag="ON430_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3219300.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9760..11130)
                     /locus_tag="ON430_001376"
     CDS             complement(9760..11130)
                     /locus_tag="ON430_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3219301.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11368..12114
                     /locus_tag="ON430_001377"
     CDS             11368..12114
                     /locus_tag="ON430_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3219302.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12111..12950
                     /locus_tag="ON430_001378"
     CDS             12111..12950
                     /locus_tag="ON430_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3219303.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12951..13901
                     /locus_tag="ON430_001379"
     CDS             12951..13901
                     /locus_tag="ON430_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR3219304.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14042..14794
                     /locus_tag="ON430_001380"
     CDS             14042..14794
                     /locus_tag="ON430_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219305.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14813..15964
                     /gene="queG"
                     /locus_tag="ON430_001381"
     CDS             14813..15964
                     /gene="queG"
                     /locus_tag="ON430_001381"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR3219306.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5251 a         3010 c         3243 g         4663 t
ORIGIN      
        1 aatttcgtaa aacaaccgta acggttgtgt aaaaatatca ttttagagca taaaaaaaag
       61 gagtggattt ctccactcca aaaaagtcta acttttgggg gtcactacag tgtcacagtc
      121 ctttttttag taaaaggata catttcttcc ttgtatattc catgcgtgtt cgtgagaaag
      181 catatataat ttaattaaca gatagttagg gggagaaaaa atgatttttt caattacaat
      241 gaaactggtg ttcgggttga tcggtttatt attagtggta cgtttgcttg ggaagaaatc
      301 catgtcagaa atcacaccgt ttgatttagt ctatacgctt gttttaggtg ggatcttaga
      361 agaatcaacc tatgatgaca atgtccatgt agggcatgtc ctatttgcca ttgcgctttg
      421 ggcagtaatg atttatggta tcgaacgtat cgtccaaaaa aatgaaaagg tcaatcgttg
      481 ggtcaaaggc gagccatctg ttctaatcaa agacggcgtc atcaacatga cagagttgac
      541 aaataaccat atcgagatgg agcagttgcg agcaatctta agacagcaag aatgcttttt
      601 tcttgaaaat gccaaacatg ttatcctaga gaatgccggt caaatgagtg tattgaaaaa
      661 atcagatgaa gacaaagctt tatcgatcct gcttgtagat gaagggcaaa ttcagcataa
      721 agtgcttcag tccaatcaat tgactgaagc atggcttatg gagaacttga agaaagaagg
      781 ctatgcagat gtaaaacagc tgatttatgt tgaatggtca gaagaaaaag ggctctacat
      841 actaaataaa gaagacgtca tgcatgaatc ttaccgaata gatggataga tagacagagg
      901 ttgtgaaatc agcgccttgg cttgttactc aaaactaagc gactttttca caacctctga
      961 tatctaatat acgttgttcc agaagttgct tcttcgaaaa ataagccaaa attccacaaa
     1021 aatttaagaa gcaattttcg aaaaatcctt tcttattttt tgaagttgat cacttctgtc
     1081 acaacctctc atgtcacaaa ccattttttt gtttccttta actcaatata ccgtacgtct
     1141 tcccacaata tcggctgatg gtcaatcata aagtaatctt cataggcttc accaatgaag
     1201 tgcccttcga cctcatccaa gagtcttcca aattcatctt ttaaattgag ctgaatgatt
     1261 actgctttac gagataaaaa ggcttctgtc agtatactat cgatttcttg caatgtcatt
     1321 tgttgtttag gaagaggctg tttgcgtgct tcttcttcat tttttttgat accgttgact
     1381 agttcttcca tcgcaaaagc agtcccccat tttaatccaa atggccgatc tcgatactcg
     1441 ttatagtcaa tgaatttttt tgctgtccgt ctcatctttt ccaccttcta atccactcat
     1501 accacctgca tgcccgccaa cgagagaact acgtgcgatg gcgcgtcctc cttgcaagag
     1561 actatgcgca tggatgattg attgaaagcc gtattttttt ctaatcgtat cgacaacaaa
     1621 atcaactttc gcttcattta tctgcttttc aggatcttca aaaaggttaa gctgtaaacc
     1681 tgtcgcgtat acgaggtcgg aacagttcac gcctacacgc cgaatatcct ggtagtggta
     1741 atgctggtgg aaaatggtca ataaagcatc tgctagatct ctgctagcat ttgtcgcttc
     1801 gattttcatt tgctggtgaa acccggtctt gccatttgga tcaagatagc ctaaagagta
     1861 tcctacccat aagctgacta cttgtgtttt tgctgcagat cgtctcaagc gagtgccgac
     1921 ttggtcggcc atttctttga taacgatctc gatttcttct cgtcttgtat aatctctatc
     1981 taggatttga ctatttccga tactttttga ttttggtgta tattcttgtc ccaaaaaact
     2041 tcgatcgatg ccccaagcat gggcataaag ctgagtaccg atgaccccta gcttgctttt
     2101 caagtgataa taatccgcgt gagctaaatc ataaacagaa tgtataccca tttgtttcag
     2161 acgttttgct gttcgtttgc cgattcccca aaagtcagtg atatctgaga ttttccataa
     2221 cttttctggt acatcttcat aacgccattc agcaaacatt tgctttccgt cttttgagcc
     2281 attgtctaag gctagtttag caagtagcgg gttatcgcca attcctatag tcgtgtagat
     2341 tccggtctgc tcaaacacat ctttatggat cagtttagcc atgtcgtacg catttgtttt
     2401 accaaagagt gccatgctat ttgttacatc aaggaagctt tcatcgacgg aatagacatg
     2461 atggttcgct tcgtctgcat attttttata gatagaattg atttctctgt tcttttccat
     2521 ataataggcc atccttgggg caacaatata taaatcactt ggatagggga agggaagatc
     2581 tctggcacgg ctgatattgg tgatgccgta agcttttttt gctgcaggac tggaagccaa
     2641 gattaatccg ctaccccgtg tttctgtcga atcggacgga taggacatga ctactaattt
     2701 agttgtaagg ggatcaagat ccttttcaac gcactctacg gaggcgtaaa aagatttgca
     2761 gtcaatacat aaaatatctc ttgaaggttc tttttcataa acaaataccg gattttgatt
     2821 cgcccacata acaatcacct cttcaacaca attagaacac acgttctctt tccgtgtcaa
     2881 taaaaaacag caattttcaa atacgagcaa aaggctcaca tttggaaatt gctgagaggt
     2941 tatttttatc tatttgtttt tagctgagat cgttgatttt tttatcaaac atgaacagct
     3001 tctggatcga tctttgttgt cccatttcca atgtgtgcaa cataaaattc cggagcatag
     3061 ccgacaactt tcatgtattc atcatagaca ttgtttttga aggcaggtat attttcttct
     3121 ttgacaagag caatcgcaca gccaccaaat ccagctcctg tcattcgtgc acctaagaca
     3181 ccttcttgtt tttgagcagc atctactagt gtatctaatt cgatcccagt tacttcataa
     3241 tcatagcgaa gagagtggtg agaatcattt aacagttgac caaatgcttc taagttccca
     3301 gcttctaatt cagcttttgc cttcaaagtc cgctgatttt cagaaacggc atggcgagct
     3361 ctgcgtatca atgtatcatc gccgatttgg tcaatagttg attcaaattc ttcttcatct
     3421 aagtctccta aagaagagat aggcaaagtt ttttgcaaac gtgccaaggc ttcttcgcat
     3481 tcgctacggc gttcattgta ttttgaatct gccaattccc gccgcttatt cgtattcatg
     3541 atgacgattg cataaccatc tagtttcact ggaaccattt cgtatttcag cgtatttgta
     3601 tctaataaga ttgcatgatc tagctcaccg aagccgatgg caaactgatc catgatacct
     3661 gaattgacac cgatgaattc attttctact tttttacctg tttggactag ttccaagcga
     3721 tctgttttta aatcaaacag atcttctaat acaacaccta ctaataattc taatgaagcg
     3781 ctactggaaa gtcctgcacc attagggatt gttccttcca ctaataactc aaacccatga
     3841 tcgattgagt gtccagcttc ttttaattta aggaccattc cttttacgta atttgcccag
     3901 tttgcttttt tatcatattc tagctcatct aaagtaaaac taatgacacc gatatcgtca
     3961 aagtttgtgg aataaactaa tattttatta tcgtctcgag gtgctgctac accttgcgtt
     4021 ccataagtga tggaagcggg gaagacatgg cctccattgt aatctgtgtg ttcaccaatc
     4081 aaattgatgc gtcctggtga gaagtattgt gtagtttttt taggtccgaa tgtttcggca
     4141 aattttgtgt ttaagcttct tttgatttcc atgatgagtt attcctttct tgttttgatt
     4201 ttgaattctg tcactgtttc aaatactgat ccggctttta aatgaatcga accaaatgct
     4261 gagaagcgct ctgcacctgg agctgtttga gtctcaaatg tgatgccacc gtgatgtgcc
     4321 agttttcggt tgcgcatttc tggtgtttct gtgccgaaat ttgcagtgaa aataaccaca
     4381 ctagaagcat ctgtagcaat atctacttgg attcttttat cggggctagt caagcgagcg
     4441 gcttctttac ccaagccggt ttcttttaag aaaaatggat gatcgattcc gtcaacaaga
     4501 tttttttgat ccaaatcact agcaaaaacg gacgctaatt ttttaggtat ttgaaaatcg
     4561 aaagcacttc ctgcagcatt ttcttttacc cctataggaa tactatctgt tcgcaaagga
     4621 gcataagctt cgctattgag ccaaagctca tgttgatcga ttgactgaga agcatctccc
     4681 gtcaagttga agtaaacatg atttgtcgga ttgaataaag tatcttggtc actggtgccg
     4741 cgagaggtca ctcgccagat attatctttt gtcaaagtat agcggatttc cacatccatt
     4801 gtacctggaa aaccatgttc tccgtcggga cttgtagtag taaaaatgac agaagcttca
     4861 ttttcgccat tcaaaatgac ataagaccat ttttttaatt caaatccagg agcaccacca
     4921 tgcagattat gtccagtttt tggatctgtg gctaattggt aagtttttcc attgagagaa
     4981 aagtggccat tttcgattct ccctgcggtg cgaccgatag aagcaccgat atacggatct
     5041 ttttcgatat attcttcagc ggtgtcaaat cccaaaacta attctcgatc atttgatttt
     5101 aaactgacga ttctagcacc taaatcagag atagaaagtg ctagcttgtt tttgttctcc
     5161 aaggttatca agtgataacc ttggccaaaa tctttttctg taatcttcat tttcaagatc
     5221 ctttcttaat gggaaggaac tactgcatcg tgaagttcat cttccattgt atcagctgga
     5281 gccaatttgt tttccaattt ctcaacgatt tgtgcatgtt ttttctctgt caatgtaatc
     5341 ttccaagcaa aaataatagc ggagaaaatc atcaaagcag caggagcata gaatgcataa
     5401 gctttgaaag tcgttacgcc atgagcagtg atatcagcag cagtagcatt accagtcatc
     5461 ccagaagcga tagcgacaaa ccctacgatc ccgttagaca aagcaccagc gattttatct
     5521 agtaaagggc gaattgatag agtaacagct tcgttacgga taccattttt ccattgacca
     5581 tattcaacag agtctgtgat cgtcatcaaa gcggataaga aaatcaattg ttgagggaag
     5641 tagaacaata ctagcccgat gataacgatt ggcaaacttg aacctgcgat cgtaaagaaa
     5701 aagtaagaag aaatcatcat tgcgatccct ccaaggtaaa cgtagcgacg agagatgacg
     5761 cgtgtaagca ttgggaatag tggaacagct actaaaccaa gtaaggcagc aactagacca
     5821 acgatccaaa attcagttgg ttggccaagt acatatttga agaagtagaa taatacggca
     5881 gttgttgcta cattggcaat cgcataagcg atataagcaa gggcaagcca catcaactga
     5941 tcattttgag caactgcttt gaacacatct ttgatcccag ctttttcatt ttgctgacgg
     6001 atcgcagtat cgacttcttt tgtgcccaag gcagtgataa tcgcagtacc gcctgaaata
     6061 acagcagcaa tcacaccgaa ccagaaccag cctgaagcgc cttcaatatg tttatgagta
     6121 acaagatagg taaagaaagt aacgataggg acaacgacta gagtagtacc atttgcacct
     6181 agagaagagc cgaaacgagc aaacgtagca aatttttcac gttcctttgt ttccgtactc
     6241 aaagctggaa tcattgacca aaaagcgata tccttaaatg aataaaaact atccaataag
     6301 atgaaaacga ttgcaaataa tatgataaat aaagtcgtat tatgagtagc taagccaaag
     6361 aaattggtaa agattgctac caagagtacg gaactaacaa cgccgccgat aactaaccat
     6421 ggtttgaatt taccgtattt tgtacgagta ttatcgatga tgccaccaat caaaggatca
     6481 aaaataattt caactaagcg gatacctaca actaagcttg taactgtagc aatcattttt
     6541 gcattcgttg ccttatctgt tcctgcgaac atctggcttg tcacgaatac cataaaataa
     6601 gtgcttaatg taacatagaa tgtgtcgtgt ccaaaagcgc ctaaagcgta ggacacacgt
     6661 tggatcattt tgcttttcat aatctgtatt cctctcctgt tcaatatata atactaagta
     6721 aaccggttac aacgaataac aaagatcttt taatgactta aacttaacaa gaaatcgctt
     6781 cctttttaat atatttctgt gacttttatt taactttttg ttgcattata ctaaaggagt
     6841 aaggagggat agacgtggag caatcgattt ttttaaaaaa ggaacatcaa caagtagaaa
     6901 aagaattgta ttttgatttt tgtggtttct ctaaaacgat gccgtttcat tcttttggtc
     6961 ctgctattcg caatgattac atcctgcatg tcgtgatgga aggcaaagga tcttatcatg
     7021 tgaaaaatca acaattccaa ttgaaaaaag gtgatctgtt cttgattcgt ccaggagact
     7081 cgacgtttta cttatcagat ggagaagatc cgtggatgta ttgctggctt tcatttggtg
     7141 ggagtgctgc agaagaaatc attcggcagt cgcttttcag agaagaccaa tacacgatgg
     7201 tctcttctga catacaaaaa tatatcgaca ttattttaga atgcatgaaa gatacggaag
     7261 aaaccttgga aaatgaatta cagttaacag cattaaccta ccgtttttta tcagtccttt
     7321 taaaagatgg cggaaaggtc agcggtggtg tacaaaaggt tcattcctct ttagctgttg
     7381 aaacagtcaa atacatcgag gcacattatg ctgaaaaatt gtctgttgag gaaatcgccc
     7441 agcatttagc ggtcaatcgg agccatttgt cgcgagtatt caaaaatcat atgggtacca
     7501 gtatcaagga atatttgatc ggggtacgga tcaatcgagg ggcgtttctt ctttctctga
     7561 cggatgaatc cgtggaaagt attgcctatc aagtagggtt caatagtcta gtcgtattta
     7621 gccgaatgtt caaaaagagt accggagaaa cggctacgaa ctatcgtaaa cgtatgaaaa
     7681 acgaaaattt taagaatatt gctttggata ccttgaaaaa acagttggaa aaacaaaccg
     7741 cagtttcttg gacaacctga ttagtctggt taaaatgaca agcatcggca aaagaaactt
     7801 gtggcggtta tgctacactg taaagcgaag actaaaaagg aggaaaaggg atgcgtacac
     7861 ttatcatcat tagccatcca acgatcgaag aatctttaaa tcagcaattt ttcaaagaag
     7921 cttctgctgg tttaccagct acttggcatc atttggaatc aagttatcca gacggaaaaa
     7981 tagatataga gactgaacta cagctcttgg cccagcatga tcggatcatt ttccagtttc
     8041 cgttttactg gtatagttct cctgctcatt taaaaatttg gcaagataat gtcttggaac
     8101 gagcagcaca tgtattaaaa gggaaagaac taggaatcgt attgacaaca ggtgtggcgg
     8161 aaaaagaata ccaagctgga ggaaaagaag aatatacgat ttccgaattt ttgaggccgt
     8221 atcagcgaat cgcaaataaa ttccatatga cctatcttcc gccattcgtt ttagcacaat
     8281 ttatgtattt atcccaggaa aaacgttggg agaagctaat tgcttatcag cagtatctct
     8341 cattggaagg caaaccatcg ctgactcagc ggattgactg gtttattcaa agagtacagg
     8401 aaaatcagaa aatgcaggaa gaagaatctg aaaaacaagc atatatcgta gaagcactga
     8461 ctgaggcaaa agagcagatc gaagacttga cctttacact gcaagaaatg aaaggaactt
     8521 cattatgaca aatgattggc tgatccaaca attagatgaa ttaagcgaaa aatcagaata
     8581 tcaagaccgt gcgctcttat atgaaataaa gaaactgatg gctgagatag acgaacgaac
     8641 aatgcagctg caaggagaaa tcgacggaca atcttggaat catctcaact ggtgaaattt
     8701 tcttataaat aacaaagata aaaatgtgca cagcgacaat tatttgctac aataagaata
     8761 tgaagcgttt gataacgcta ttatacataa aaattggtgg tgaaaaaaat gtatgatgaa
     8821 aatgagtggg gagaaaatgt tcggatgacc cgaagaagac agcaaaaaca ggcagctcgt
     8881 aagcggatga tcatgattgg ggcagcagtt gccgtgtttt tgatcatagc aggaggagtt
     8941 gcgggtctat attttcatgg aaaaaattcg tcagaagcga aaagtacgac cataacggaa
     9001 acaacgacaa agaaaacaga gacgaaacaa acaagtgagt catcaactaa aacaaaagag
     9061 acacaaactt ctgaagaaaa aacagcaaat ggtttgccag agcaacttca agcaatcgta
     9121 aatgattatt cgaaaaagat cgaagaaaag acgcctgcat tgattgaaga ataccagaca
     9181 gaaattcagg gaaatcaaga gggaatagct ggtttatcag cagttgctaa ccaaaaagca
     9241 cgtgagcttc aagcaatctc cgacgaaggg atcagaaaat tacgtgcagc gtatcaggca
     9301 gcagaaaata aagacggtgt agacttagat actttgatca atcaattatc ggctaattat
     9361 acgaatcatg tcgctaagat ttccgatatt tatttgcaga ctagcgcctc tcttcaagca
     9421 gagtcaacaa gcacacaaga cacaacatct tctgattctg agacagcaga gtcggtagaa
     9481 gcttctgaaa tgcaagctcg aacaacacaa gacacaacag attcctctga aactaatacc
     9541 aacagcgcga cttcacaggc tgcgacaacc gtagttttgg aaggagaagg tccaaatcaa
     9601 atagccgaac gaactggcgt accagtagaa acgatcttgt cgctgaatgg gatgagcatg
     9661 gagaattatt tcctgactcc cggtgagacg ctaaaattga attgataaaa gcaataaaaa
     9721 agaacgaagc ttccactaag gtgaagcttc gttctttttt tattttccgg ttcttttttc
     9781 gcgtacaaag taatggatcg tcatgtaaag caagaaaacg agtccaataa tcaatgagat
     9841 tctcgtttca ggattgataa acatgaaaat caatacgata gctaaaaaag caagtgtcac
     9901 ataattcgta taaggagata gtggcatttt aaaaggatga tcatgcattt tatctgcgtg
     9961 cttctttttg aagcgaatct cgctgatcaa aatcactacc caaggaacca taccaggcaa
    10021 aatacttgag ctgtatacga aaacgaaaat attttctgac tggttgatga acattggtag
    10081 aagataattt aagatcagac caattaagat tccacctgag attgctacga caggataaaa
    10141 aggcgcaccg tgacgagata gtttcagaaa ttttttagac atctttccat ttatggcaag
    10201 tgtatagacc atacgacttg cgctgaaaat ccctgaattg caacctgata atgccgctgt
    10261 aatcacgaca aaattaatca gtcctgcagc aaatgtaatg ccgactttag aaaaagtttg
    10321 tacaaaaggt gaaccaatct cactcaattg attccacgga tagatactca caatgataaa
    10381 aatagcacca atataaaaaa tcaagattcg accgatagtg gactggacag cttcaatgat
    10441 cgtcttttta ggattctctg cttcaccagc agtcattccg atcagttcga ttccttgata
    10501 ggaagcaaca acgatcgata atgcaaagaa aaatcctttg acgcctccag tgaagaatcc
    10561 accatttttc caaagatttg aaatccctac tgcctgacca tgattaccaa atccaaataa
    10621 gattacccca agacctgcaa tgatcatcaa aatgatcgtt atgactttga tgatcgaaaa
    10681 ccaaaattct aattcgccaa acgccttgac agaaataaga tttgccagca taagaaacaa
    10741 gacgacaatg attccgggaa taattgccgg caaatcaggc caccaataat tcatataaga
    10801 tcctacagca atcacttcac ttattcctac gacaatatat tgaaaaatat tactccaagc
    10861 ggtcaagtag ccagccacag gatggatgta ctcgctcgcg taatttgcaa aagaaccagt
    10921 cgaaggatca acgtaaagca tttcgcctaa agcacgcata atcaaataca aaaagaatcc
    10981 tgcaatcgca taggctccca tcactgaggg ccctgtccaa cgtatggttg aggaggcacc
    11041 cataaacaga ccgacaccga tcgttccacc tagtgcgatc atttggatat gacgggcact
    11101 aagacttcga gttaactttt cttcttccat acaaaaactc ctttcgttgt tgagtcaaca
    11161 agaaaaaata atcaaacgtt tcaacacatg tttttcatta ttaggttagc cttattgaac
    11221 tcttgatgtg attctacaga aaattttcat aaaatgcgag gaaaattcga aaaaaacttt
    11281 ttcatcattc tacttgaaaa tgctttccat tttagttaaa ctatatttaa ataaaaagtt
    11341 aggtaaacct aaaaaggagt gtaagatttg gaacgtacgg ccattgtaat tcaagacctt
    11401 tcagccgctt atcaagggaa aactgttttg aaaactatca acttgacgat aaatccacaa
    11461 aaaattaccg gtattatcgg accaaacggt gcaggaaaat caacatttat gaaatctttg
    11521 ctagagttga taccagctac tactggtaaa gtcacctttt ctggacagtc ggtaaactca
    11581 gtccgtaaaa aaatagccta cgtggaacag cgaagtgaat tggatctttc ctttccaatt
    11641 gatgtattag gcgttgtact tttaggaaca tatccatctt tacgaattgg acaaagacct
    11701 gggaaaaaag aaaaagaacg tgcaagacaa gctttgaaaa aagtagggat ggaagaatat
    11761 gccaaaagac agatcagcga actatcgggt ggacagcttc agagagtttt tattgcaaga
    11821 gctctagccc aaggagcaga atggatcttt ttagatgaac cattcgtagg gattgatgcg
    11881 ttaagtgaac gaaagatctt tgacatcttg caggaattga agaattcagg aaaaacgatt
    11941 ttgatcgtcc atcatgatct tcataaagta gaagaatatt tcgatgaggt tattctttta
    12001 aataaacagc tgatcgcttc cggtccagta caagaagtct ttacatcgaa gaaccttcaa
    12061 ttggcctatg gtgagatcat cagacattta gtaaaaggag gagagaaaaa atgatccaat
    12121 cctttattga tggattaatg aattatcaat tcctgcaata cgctttggtc acttccatgc
    12181 ttgtaggact tgcttctgga gtcatcggtt cttttattat tttacgaggg atgtctttga
    12241 tgggagacgc tatctctcac gcagttcttc caggtgtagc catttcttat atgtttggtt
    12301 ttagttacat ctttggagcc actgcttttg ggatgctaac agcagcagtg attggtttcg
    12361 tgactcagcg gagccgattg aaaaatgata cagcgattgg aattgtcttt agctcctttt
    12421 ttgcattagg gattatcttg atttcatatg cccagagtgc gacagatttg tatcatattt
    12481 tatttgggaa tgtcttagct gttagggaaa gcgatttgtt gctgacagca ctcgttagtg
    12541 gaatcgtctt gatatttgtc tttttctttt ataaagaatt gaagatcaca tcattcgatc
    12601 cgacaatggc aaaagcttat gggctgaaca catcgctgat ccattatctt ttgatgttct
    12661 ttttgacatt agtggctgta gtcagtttac agacagtagg aacaatcttg gtgattgcca
    12721 tgttgatcac accagccgcc acggcttact tgctaacgaa ccatttactg aaaatgatca
    12781 ttacagctgc aggaatcggt atgctaagtg cagttgtcgg tgtgtttttc agttatagtt
    12841 acaattggcc atcaggagct acgatcgtgt tagcatgtac cgcatttttt atccttgctt
    12901 ttttattttc tccaacaaaa ggaattttat ttcagaaagg aagcagatag atgaaaacaa
    12961 aaaaatcact ctttttaatc ttggcagtta gtttcttggt attagccggt tgcggaaagc
    13021 aggcttctga ccaagcggat gaaggaagta aagaaaagtt gtcagtcgtg gctaccaatt
    13081 cgatcttggc ggacatggca aaagaggtcg gaacagatca aatagatatc cacagtatcg
    13141 taccagtcgg aacagatccg catgaatatg aagtactacc agaagacatc aaaaaggcaa
    13201 gcgatgcaga tgttatttta tacaacggtt tgaatcttga aacaggtaac agctggttcg
    13261 ataacttgat ggaaacggct aaaaaagaag aaggaaaaga ttattttgca gttagcaaaa
    13321 atgtagaacc tctttattta actagcggtg aagaacatac aaaagcagat ccccatgctt
    13381 ggctagacct atctaacgga ataaaatatg tggaggaaat cgcacgtata ttctctgaaa
    13441 aagatgcaga aaatgcgaca ctctataaaa aaaatgcaga agcatatgtg gaaaaactaa
    13501 aagaattaga tacacaagcg aaggaaagtt ttgcttctat cgaagagaac aaaaaattat
    13561 tagtaacaag tgaaggggca ttcaagtatt tttcacgagc atatgatttg ccagcagctt
    13621 atatatggga gatcaataca gaaagtcaag gtacgcctga tcaaatgaaa gcgatcattg
    13681 atcagataag agcaaaagaa gtaccggttt tattcgtgga aaccagtgta gactcaagaa
    13741 gcatggaacg ggtagcaaaa gaaacgggtt tgaaaatcta tgataaactg ttcactgatt
    13801 ccatagcaaa agaaggagaa caaggagatt cttactatca gatgatgaaa tggaatattg
    13861 aaacgatcca tgaaggtttg tcacaaacaa aagaaagctg aaaagtgcaa ggtctagctt
    13921 gaggagcaga ccttgcattt tttataagag atatgaaaaa aaattatgct gtttttcgtc
    13981 gtacagatac cttttttgaa gaaattccgt tatagttaga gtaaggaagg aaggtgtggc
    14041 tatgaatttt acacgtaaag ataaatatac gattaaagaa gaagtctttc gattgatcag
    14101 ccagcaggaa aataccgaca aggatcaaga ccttcaattt cttgaagcag aattagctaa
    14161 attattcata gcaaatcaaa atgagacaac agaaccagat gaagagctgt acataggcaa
    14221 atggttgttt gatgaatttc agcctttaga tgaaaatacc ggtatcggtt attttgtgtt
    14281 cctttctcca gtcggcttca cacctggcgt attagttaaa caaaacgacg aaataaccgt
    14341 caaagcatgg cctgcattat tttcagtcat caaagcagaa ggagtaatca aaaagatcca
    14401 gtcggcctat tataaaagtc aatttattgc caacagcaaa ttaatgaata tgttttataa
    14461 tcgtctagta gaaattgcta ggcctgggaa aatcccgcaa acattcacgt acacacagca
    14521 aaaaaatctc tatgaagaag tattgtattc ccgcgatctg tctgagtttt cgccatggga
    14581 agaggtaagg ctccatgaac tgttaagtac aaaaccagtt tcattgaatt tttttaatgc
    14641 attagataac aaaagaaaat taatttcaac aaattattgg gaagtatttg ctgcaagaaa
    14701 aaaatggatg gaagaaaaga agattgccga tcagcagatc cagtttgtcc agctgaaaaa
    14761 agaaaaacta tattatgggt tatacagccg ttaacagcaa gcgaggaaga ccatgacatt
    14821 aaaagaagaa atcatacaag aaagcaaacg attaggaatt gacaaaatcg ggttcaccac
    14881 ggcggaacct tttgattcat taaaagaatc gttggaagaa caaaaagcag ctggccatac
    14941 ttctggcttt gaacatccaa atatcgatga gcgtctttat ccagaaatga catttgatca
    15001 gccgaaatcg atcatttcca ttgcacttgc gtatcctact aggatccatg aagaagtgcc
    15061 tagggatgaa aaacgcgggc agtttgctcg tgcttcttgg gggatcgatt accatgatat
    15121 actcagagac cgtttgaacc gattgatcga attcatccaa acaagagcag aaaaatggca
    15181 aaaagaagag gaatggcgtc tagctccgca agtagatact ggagaactga tcgatgtcgc
    15241 tgtagcacag cgagctggtt taggatttat tggacgaaac ggattgttga tcactgaaga
    15301 gttcggttca tttgtctatc ttggtgagat cgttactaat attgtatttg agcctgataa
    15361 accaggagaa tttggttgcg gtgactgtat gcgctgcgtg actgcttgtc cgacaaaagc
    15421 attgcttggt gacggaagaa tgaatgccca gcgatgtttg tcttatcaaa ctcagacaaa
    15481 agggatgatg ccggaagaat atcgtaaaaa aatgcgaaat gtcatttatg gatgtgatat
    15541 ctgccagtta gtctgccctt acaatcaagg aaaagatttt cattttcatg aagagatgga
    15601 accaaaagta gacgaaatat atccaaaatt aaagccaatg ctttcgatgt ctaataaaga
    15661 cttcaaacag caatttgggc atttagctgg ttcatggcgt gggaagaaac ctttgcagag
    15721 aaacgcattg attgctttag ctaatctagg tgatcgaacg gcattgcctg agatttcaga
    15781 atgtttaaca gatgtccgcc ctgtcatcag aggaacagca gcttgggcta ttggacgtct
    15841 aggaacaaaa gaaccggaaa aatggcttga tgtattagat gaaatattag ctaaagaaac
    15901 agaagaagtc gttataaacg aattggaaca tgcccagaaa ttattgcgga gaaagttaaa
    15961 ataaaaaaat agttaacatt attcaaagtt tttgtccagt aattgttgta acaattactg
    16021 gtttttttgt attttttaca tttttttaag agaatttgat aaaaatcaaa aatttttttg
    16081 ctttttttta tggattattg cagtgaatgt tacagaatct gcgtttttca ttcaaaaata
    16141 aaaaaacata gaaaaacgat tactttg
//
LOCUS       DAJSMI010000056        16027 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJSMI010000056 DAJSMI010000000
VERSION     DAJSMI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.056"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            100..1602
                     /gene="eat(A)"
                     /locus_tag="ON430_001382"
     CDS             100..1602
                     /gene="eat(A)"
                     /locus_tag="ON430_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR3219307.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1630..2196
                     /locus_tag="ON430_001383"
     CDS             1630..2196
                     /locus_tag="ON430_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3219308.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            2407..3468
                     /locus_tag="ON430_001384"
     CDS             2407..3468
                     /locus_tag="ON430_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3219309.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3471..4367
                     /gene="murQ"
                     /locus_tag="ON430_001385"
     CDS             3471..4367
                     /gene="murQ"
                     /locus_tag="ON430_001385"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR3219310.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4381..6363
                     /locus_tag="ON430_001386"
     CDS             4381..6363
                     /locus_tag="ON430_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3219311.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6428..6874)
                     /gene="nrdI"
                     /locus_tag="ON430_001387"
     CDS             complement(6428..6874)
                     /gene="nrdI"
                     /locus_tag="ON430_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR3219312.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            7085..8509
                     /locus_tag="ON430_001388"
     CDS             7085..8509
                     /locus_tag="ON430_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3219313.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(8690..9463)
                     /locus_tag="ON430_001389"
     CDS             complement(8690..9463)
                     /locus_tag="ON430_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3219314.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(9460..10365)
                     /locus_tag="ON430_001390"
     CDS             complement(9460..10365)
                     /locus_tag="ON430_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3219315.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            10531..11349
                     /gene="purR"
                     /locus_tag="ON430_001391"
     CDS             10531..11349
                     /gene="purR"
                     /locus_tag="ON430_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCR3219316.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            11852..13225
                     /gene="glmU"
                     /locus_tag="ON430_001392"
     CDS             11852..13225
                     /gene="glmU"
                     /locus_tag="ON430_001392"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR3219317.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            13319..14290
                     /locus_tag="ON430_001393"
     CDS             13319..14290
                     /locus_tag="ON430_001393"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR3219318.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      14296..14398
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            14464..15033
                     /gene="thiT"
                     /locus_tag="ON430_001394"
     CDS             14464..15033
                     /gene="thiT"
                     /locus_tag="ON430_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCR3219319.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(15114..15887)
                     /locus_tag="ON430_001395"
     CDS             complement(15114..15887)
                     /locus_tag="ON430_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219320.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         5147 a         2833 c         3445 g         4602 t
ORIGIN      
        1 tttttcgatg tatgggtctt tttgtgtgta tccaaccctt gcatcctctt tagccagttc
       61 tgaaaatagg actggtcttt ttttacgagg tgatggaaaa tgtctaaaat cgaaataaaa
      121 aatctgacat tcggctacga cagccaaggc acattattat ttgaacaagc aaatctaaat
      181 tttgacacac aatggaaact aggacttatc ggacgaaacg gtcgaggaaa gacaacttta
      241 ctgaatattc tacaaaacaa actaccttat caagggcaag taatccatca gcaagaattt
      301 gcctatttcc cgcaacagac aaaagataaa gaacgtttaa cctattacgt gttaaatgat
      361 attacggatt ttgagatatg ggaaatcgaa agagagctcc aattgatgca aacagatcct
      421 gaaatcttat ggagagaatt cagcacacta tcggggggag agaagacaaa agtcctactg
      481 gcacttttat ttgtggatga cactcatttc ccgttaatcg atgaaccaac gaatcatttg
      541 gatatctctg gtagaaaaca agtagcggct tatttgaaaa agaaaaaaca aggcttcatc
      601 gtggtcagcc atgaccgggg atttatcgat gaagtagtgg accatgtttt agcaatcgaa
      661 aaaagtcaac tggaacttta tcaagggaat ttctctatct atgaagaaca gaaaaaactt
      721 cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa aagaagtcag taggctaaag
      781 aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag aaggagataa aacaaagaaa
      841 caagtcggat tcatcgatac tgaatctaga cgagtgaata aaggagcagt gggtgctgat
      901 gctgcacgga cgatgaaacg atccaaagca atcgtgaatc ggatggagac ccagatcagc
      961 gagaaagaaa aactattaaa agatatcgaa tatatcgatt ctttgacgat gaatagccaa
     1021 gcgtctcacc ataagcgact tttaagcgta gaagatcttc aattagggta tgaaaatctg
     1081 ttattcgagc caattcattt tacaatcgag cctcatcagc gggtggcgat ttcaggtcct
     1141 aacggtgcag gaaagtcatc cattatccat tatcttctgg gggcattcaa cggcaaggtt
     1201 ataggagaaa aataccagcc aaaacatctg agcattagtt atgcaagcca aaattatgaa
     1261 gacaatcgag gaacgttggc ggaatttgca gagaaaaacc aagtagacta ccaagcattt
     1321 ttgaacaacc tccgaaagct tgggatggaa agagatgttt ttcataacaa gatcgagcag
     1381 atgagtatgg gccaacggaa aaaagtggaa ttggctaaat ctttatcaca gccagctgaa
     1441 ctatatacat gggatgaacc attgaattat ttggatgtct tcaatcaaga acaattagaa
     1501 caactgatct tgaacgtgaa acctgccatg ttactagtgg aacatgatca aaccttcctg
     1561 gataaagtat ctactgagat tatttctctt gagagaatct aacccttgta atctcaagaa
     1621 caacaaggta tggttaaggg gaactttgcc aacgaaggag agaatcacat gctttatgta
     1681 acgagacacg gagaaacaac ttggaacgca caaggattag tttgcggacg ggcagatgtg
     1741 ccgctgactg aaaaaggaca gatgcaggca cagaaactag cagaaaaggt agtagatttg
     1801 cctgttccaa tcacgaaaat catccattcc cctcttcaac gagcaagaga tacagcacaa
     1861 gcagttgctg atcgtctcag cttgccgctg accgttgatg aacgattggt agaaatggat
     1921 tttggggatt atgatggact gccaagcaaa gatgagaact ttcaaaaagc acgtttagcg
     1981 ttcgctgtac gattcccaaa tggggagtca gtcttagatg tgtatgcacg gatcgttcct
     2041 ttattgaaag aatgcatcga agacgaagaa aatgtctatc tgttagtttg ccataatgcc
     2101 ttgataagag tgatcaatgc ttattttcat ccgatgccta atgaggggtt ctttacattc
     2161 atggtagata atacagaatt ggtttcttat gagtaattat gtttttgtgt tatttaaata
     2221 aaatatttca aggcactgct tttgcttagc agtgcctttt ttaggttatg acaattcttt
     2281 tgataatata aactcaacct ttagaaatga aatttaatta ttttaataaa agtattgaaa
     2341 ttttgtttct ttagatatat actctagtca tagaaatgaa gacggataca gaaaaagagg
     2401 gataatatgt ttggcttttc aatatttatg aacgattctt tgtcagaaga gaagaaacag
     2461 tatatccgcc aaatggcatc caatggcttt gtcggtattt tcacttccat gcatatacca
     2521 gaagatgatg tttcggcata tcaaaagcga ttgatcgaac taggaagcga agcgaaaaag
     2581 aataaactag aattgatggt cgatatttca ggagaggcat tggaacgagc gggtttttca
     2641 ttagaagatc taacgccttt gaaagcaatt ggtgtaacag gtttaagaat ggattatcat
     2701 atttccaatc agcagattgc cgactggtca catcaattga aaatcagttt gaatgctagc
     2761 acgatcacgc ctaaagatat cgatgaattg aaagaagcag aagctgattt ctcacagatt
     2821 gaagcttggc ataattatta tccacgtccc gaaacagggc tggacaaaga atggtatcag
     2881 aaaaaaaatc aatggctcaa aattcaaggt ctccttgtcc aaggttttgt accaggagat
     2941 acagagttga gaggcccact atatcaagga ttgccaactt tggaagaaca tcgagggatc
     3001 catcctcttg caggcgctct agatctatta gcaagtaaca cggatattgt ttacatcgga
     3061 gatgcaggtc tgtcagaaaa cgttcaggag caatttgctt ctttccaaaa agaacagaca
     3121 gtcttgctcc atacggaacc tgtagatgaa gaattttacg aatatatatt agggaagcat
     3181 acgaatcggc aagacgatgc aagagatgtc atacgaagcg tagatgcacg ttttcgagag
     3241 attccgccta tcccagcaag aaatacagct acgcgtatga aagggtctat tactttagat
     3301 aacgaaaagt atttgcgcta tatgggtgag atccaattaa ccaaatatga tttgccagcg
     3361 gatgaaaaag tgaatgtagt agctaaagtt atcaaagaag agctaccatt gatcaaccaa
     3421 atcaaagctg gtatgaacta tcaatttata agaaaagaag gaagataaaa atgagcaaaa
     3481 tcaatttaga gaacctaaca acggaacgca gaaaccaaga aacttttgga ttagatgaga
     3541 tgagtatttc tcaagccgtt caactaatga acaaagaaga tcagaaagtg gccgctgcgg
     3601 ttgaaaaaga actgacaagt atcgaaccag tgattgctaa cacgattgag tcctttaaaa
     3661 aaggtggacg tctgatttat atgggagccg gaacaagtgg aagactaggt gtgttagatg
     3721 ctgctgaatg cgtaccgaca ttcggtgtag aaccggaaat ggtcataggt ctaattgctg
     3781 gtggagaaga agcaatgact gtagctgtcg aaggggccga agattcttat gagctagggc
     3841 aagaagatct aatcaagctg aatttgacaa aaaatgatat agttattggt atcgcagcta
     3901 gcggtcgtac accatatgtg atcggtggat tagattacgc aaaatcgatc ggtgctgtca
     3961 caggaacgat ttcttgcaat aaacaagccg aaatcagtaa acacgcggat ttcccaatcg
     4021 aagtcgatgc aggtccagaa tttttgacag gctctactcg attgaagtca ggaacagcac
     4081 aaaaacttat cttgaatatg atttctacta tcagtatgat tggtataggt aaagtattca
     4141 ataacttgat ggtcgatgtc aaaccaacaa atgaaaaact aatcgaacgc tcaaaacgaa
     4201 tcatcatgca agcgacagaa gctgattacg aaacagctga aaagtacttt gaagaagcag
     4261 atcaaaatgt taagctggct atcgtcatga ttttgacaaa tagtgataaa gaggaagctc
     4321 aagcaaaatt agctgaagca aacggatttg taaaagggac cttatgaggg ggaaaagacc
     4381 atggcaaaaa acgaactgac tttggaagaa ctggcaaaac aaatttataa aggggcaggc
     4441 ggcatgggca atgtagaaag cattgtccac tgcatgactc gtgtgcgtat gaagatcatc
     4501 aatgatgatc ttgtaaatgt ctctgaatta aaagcgattc caggagtgct tggtgtagta
     4561 gaagatgaac agcttcaagt catcatcgga cctgggaaag tgaacaaagt agcacagcaa
     4621 atggttgatt tggcaggcgt gaaattagga gaaaaacttt cttctgtaag tacagctaca
     4681 gcaaattcat cagcatctgg aaaagaccgt gtaaacgaac gggcacaaga aatgaaagcg
     4741 gcacaaaaat cgaaacaaaa accatctaaa atcaaatcgg tattaaaaga catttctaat
     4801 atctttgtgc caatgattcc tgcatttgtc ggtactggga tcgtggcagg gattgccgca
     4861 gttttagcaa acttagtcac agccggaaca cttgatgcag caacatggca acaatatatc
     4921 gatgtgatga acatcttgaa aaacgggatg tttagctacc tagttatcta tacaggtatc
     4981 aatgctgcac aagtttttgg tgcaacacca actcttggtg gagttatcgg ggccgttgtg
     5041 atgctgacag ggatgaatcc ggaagcacca ctgaaaaact tatttactgg tgaagctctt
     5101 gcagctggac aaggtgggat cctaggtgtc atcttcgctg tttggctatt gtcaatcgtt
     5161 gagaaaaaac tgcataaagt agtacctgat gcggtagata tcatcgttac accaactctt
     5221 tcattgattg ccatcggtct gattgaaatt ttcttcatca tgcctttagc aggtttcatt
     5281 tctgatggca tggtcggtgg gatcaactgg gtacttggcg ttggtggagc atttgccggc
     5341 ttcgttctag gcacattatt cttgccgatg gttatgttcg gactgcatca aattttgact
     5401 ccaatccatg tgcaaatgat tgatgaaact ggtcgtacgc tattgcttcc aattcttgcg
     5461 atggctggtg caggacaagt gggtgcagca ttggcattat ggattcgctg caaaaaagat
     5521 aaagaattaa cagaaatgat caaaggagct ttaccagtcg ggatcctagg aatcggcgaa
     5581 ccattgattt acggagtaac attacctcta ggacgcccat ttattactgc ttgtatcggt
     5641 ggaggtatcg gcggggcagt gatcggaatg atcggaaatg tcggggctat cgctattgga
     5701 ccttcaggag ccgctttgat cccattgatc tctgacggta aatggttagg ctacatcctt
     5761 ggattattgg cagcttatgc tggtggcttc gttgctacat tcttcttcgg tattcctaaa
     5821 gaccgtttag aaaaagaaga attagcgact gaaacagcca cagtaaatga agtgcctgaa
     5881 aaagtagtac ctcaaacttc agctgaaagt gtgactatcc tctcatcagt tgctgacgga
     5941 acagttgttt ctttagataa agcaagtgat ccagtttttg cacaaaagat gatgggtgaa
     6001 ggctactttg tagaaccaga aaacggacat atctattctc cagttactgg ggtagtcagc
     6061 tctgtcttcc caacgaaaca cgctattggg atcacaacgc caaatggttt agaaatcttg
     6121 cttcatatgg gaatcaacac agttgactta ggcggtacgc catttgatgt gaaagtaaca
     6181 gaagggcagc aagtgacacc tgatacgctt gtagcagaag ctgacttaga agcgatcaaa
     6241 gcagcaggca aagaaacatc catgatcgtc ttggtaacca atatggatcg tgtaaaaaac
     6301 tttgtcttgg aaaaaacagg taaagcaaaa gcaaaagcac ctgtgatgga tgtcaaagct
     6361 taatatgata gaaacaatag aaacgaaaga agaaactgaa atctattttt cagcttcttc
     6421 ttttttatta tttattgttt tctgctaaaa tctgataaat tcgtgtcaca tcagatggtg
     6481 ttccccgtaa ttcataatct gccaagaaag gaaagccaaa tgtttgtgca tattgtttgg
     6541 ctgttagaca atactgatta ttgaagtttt tatttccgct accgactaca ccaagacaaa
     6601 gtttatgatt gtcttcatgt tccagatatt cacgcatcgt ttcggtcaag atttctgtat
     6661 caccgttatc taaaccattt ccgccgtcta aataagtagg aacgaaagta aaaaatggtt
     6721 ctgtttcttt cgtaaaatca ctattttcat ggatttcttt taaagtaact tcaggattga
     6781 caggatcttc tgcatgcatt ttttcagcat attctgctag atgtttggca aaggcacgcg
     6841 tatttcctga aatcgatata tataaaatgt tcatggatca tcctccaatt ctttcctttt
     6901 agtatagcat cttttttaag ttttttggtg gtgtaatttg gaaagggact aggcgctttg
     6961 cttgtataat gagaaaaaag ccggtagaat tatatttcta ttaaagtttt tattaattaa
     7021 gtaaaaataa aaaaggtttg ttatactttt tgataaaagc ggttttattt tggaggatgc
     7081 ttacatggat acagagcaac agaaaaaagg aattggttta attccacttg ctgccttagt
     7141 aattggttca gcgattggag gtggcgtatt tggtattatg acggacatct ccggatcggc
     7201 tggcggccca gctattttca gttgggtact cgtaggaaca gcaatgttga tgctctcctt
     7261 gtcgatcaat aatatcaata aaaaacgtcc agatttaaaa ggcgggatct ttagttacgc
     7321 agaagaaggt tttggccgtt tcgctggttt tatcagcggt tggggttact ggctcacatg
     7381 ctggttaggc aacgtagctt ttgcaacatt gttgatgagc gcattaggat atttcttccc
     7441 agtatttgaa ggtgggcaaa atctggcatc gatcgtgatg agtacagtct ttttatgggg
     7501 atatgcatgg ctagtgaatc ggggagtcga aaatgctagt ttcgtcaatg ctatcgtcac
     7561 attctgtaaa ttagtgccat tatttttatt tattgtagta gctatcagca cgttttcgat
     7621 ccaacaattc attgataact tttctagcga cattatattg aatgcgaatg gacagccgat
     7681 cccgttttcc agtcaagtat tgagttgcat catggtgatg ctttgggtat ttgttggtat
     7741 tgaaggagcc tctgtcgtag gttcacgagc aaaaaaacaa tcagatgttg ggaaagcaac
     7801 gattctaggg atattcggct tgattattat ttacgtcttg gtttccatgc ttccatatgg
     7861 gacaatgacg atgaatcagc tgcaaacgat caaggcgcag cctgctatgg gctatgtgtt
     7921 tgaaatgatg gttggtaaat ggggagcagt cgtgatcaac ggcggtttga tcatttcttt
     7981 agttggtgtt tggctctcat ggacgatctt accgattgaa acaatgggaa acatggctga
     8041 agacggactt ttgcctaaag catggggaaa actcaacaaa aatggggcac caacttatgc
     8101 tatcgtattg acgacgttat gtacgaatgt ctttttactt tcattattat tcactgacag
     8161 tgcttataac tttgcttaca cacttggtac agcagctatt ttcttcacgt ggcttttctt
     8221 aggattgtac caaatgaaac tttcttatca gcggaaagaa tgggtccaat ttttagtagg
     8281 gcttttagca agtgcctttc aagtatgggc tatgctagtc gtagccttta atgaagtaat
     8341 gcttgttctt gttcttttct tgccaggcat ttatttttac ctcaaagcac gtaaggagca
     8401 aaacgagcct gtacaacata tccacttaga taagatttta cttataggtg tgacgatggt
     8461 cggagtgatt gcaatggttt tatttgccac tgggatgctt cacgtataga agtataaaag
     8521 aagtataaag agaggttgtg agcctgtcgt ttattcaaaa acatactagg aaaggagctg
     8581 tgacacaaat gttgtcacag ctcctttttc agaataaacg gtgtttaaga gacagccaat
     8641 cggcaataag gctgaacgtc tctttcacag cctctttttt gatgagtcat catacatttt
     8701 ttcgcttaaa tacgagatat cctaatgcta agaacaaaac gatgtaagct aaattaccga
     8761 atagcatttg attagtagaa agatgagtca attgcttcat gatctcttcg tttccaattt
     8821 ggttttgcag attcaacata ctgatcggat tccatttgat ccactcccat ttttggatga
     8881 gtgcaataag gataccagat atgattgacg aagcaaaata aaagacgatt cctgctgaga
     8941 tggatgcgcc ggaactattg ataaagcaag caatcatcaa cactaaactt aaaatcagcc
     9001 ataaaccgat ataactaccc aaagtataag tgaacaagct ttggatcagt gtttgaccag
     9061 tagaaacctt ctccgtgaag gaaatcgaag ggaaaagaat cagtttcatc aacacagtga
     9121 caataatagc taaaaggtat aaataaacag aataaataac taatgtgatc catttgctta
     9181 ctaatatttc tcctcgagaa tactttcgat aaaggagatt tttcaatgta ccatattgtg
     9241 cttccatcga aatgatcgtg cttgcagctg caatcatgat aaagacaatc caagatgtgg
     9301 cactaaaaag ctgagcagtc atttcaactg gatcaatgat cgtcgtgtat tttttggcta
     9361 gaagtgctgt tcctatgagc aaaacaacta atagaacaga taaaacagca gttgactttt
     9421 tcttgatcaa tttaaataat tcttgtttta caagcaatgt catttttctt cttccccctt
     9481 tactgaatcc agaatttcca atagagaaga ttccaaatca ttcgtcagat gctggacgtc
     9541 tgtcacatgg atattttgtg cagacaatag ctggatcacg gttcctaata attcttcgtt
     9601 ttcttttaaa agcttgattt tatcaggatc ttccaaagta tatccagctt ctgtcaagat
     9661 cgttcgagca gctagatcat ttgttgtaga gataacgatg aaatcttgcg ctgtttggtt
     9721 caattcagcc attgtacatt gtttgatgat ctttccttga ttgatcagga tcagctcatc
     9781 gaccattttt tcaagctcgc tgagtaagtg actggagatc aagaaagagg tgccctgagt
     9841 agccaagtcg gtaatgattt ttcgcacatc tcttgttgct tgtggatcca aaccgttcat
     9901 cggctcatct aaaataacga tagacggatg gttcagtaag gcaagagcaa ttcccaattt
     9961 ttgtttcatt cccaacgagt aggatttcgc ttttttgtga atgtactttt ccatcttgag
    10021 ctgattcacg atagtgtcga tagcagatac atcgttggtt tcagagaata acttcagatg
    10081 atcatagcct gttaagaatg gatagatacc cgggtattcg atcaatgctc ctacttgttc
    10141 caacccttta tgactagagg gagaaatctc attttcacca atacagattg ttccatctga
    10201 gtaatggatc agtcctaaaa tagctttcat gatcgttgtt tttccagcgc cattaggacc
    10261 tactaatcca acgattttgc caggggataa gctaaaactg acatcgctga gggcttgttt
    10321 gtgaccaaag ttcttggata attcttggac cgaaagactt ttcaaaaata ttcactcctt
    10381 ttctatagat agtacaagca tacacctgat gagtggagaa gtcgctaggt cattggactt
    10441 atttcttatt ttttcataaa atgctcgaat ttttgaagaa aaacttatat aatgtaaaac
    10501 gataaaattt cttaaaagga gttggaatag gtgaaagtac gtcgcagtga acgtctagtt
    10561 gacatgacac agtatttatt gaatcacccg catgaattga tttccttaac aagctttgcc
    10621 gaacgttatg aatctgctaa atcttctatc agtgaagatt tgggaatcat caagaaaaca
    10681 tttaaagaac gagggtatgg aaccttggaa acaatcccgg gtgctgcagg tggtgtattg
    10741 ttcatccctg aaatttcata tgaagaggcg aaaaaatata ttgaatctct agcagaacgt
    10801 ctatcagaac aagaccgtct gcttccagga ggatatgttt atctctctga tttgttagga
    10861 gaacctgaac tgctgcgtca agttggaaga atcattgctt ctaaatacct tggtgaaaaa
    10921 atcgatgctg taatgactgt agccacaaaa ggcgtaccta ttgcccaagc tgtgtcctat
    10981 tatctgaatg cgccattcgt tattgtacgc cgggactcaa aaataacaga aggatcaaca
    11041 gtaagtgtca actatgtgtc tggatcttct gaaagaattg aaaagatgga actgtctaag
    11101 agaagtttga aacgtggttc acgtgtcctt gtagtggatg atttcatgaa aggcggcgga
    11161 acagtcaacg gaatgaagag cttgattgaa gaattcgaat cagaacttgt tggcatcact
    11221 gtatttgccg aatcaaaatt caacggtcat cgcgccatcg atgattatac gtcactgctt
    11281 tatgtcaaag atgtagatac tcaaacgaaa aatattacgg ttgtgccagg aaattatttt
    11341 tcagaataag aggttgtgaa aaagccgctt agtttcgagt agtaagaaaa attttgtgaa
    11401 aatggctttc cacatttttt cataaatttg gcttaatacc gagaaactgg cttttgaaca
    11461 ccgtttatct agaggttgtg aaatcagcgt cctgtcctga gtattagctc aataaacgga
    11521 aaaattgctc ctcatatttt gccgtttatt gagctaatac cgaaggtcag ctgattgaaa
    11581 aacgtttaat gaagaggttg taagcctacc gtttaactct aagtcacaag aaacgatttc
    11641 aaaaaacagc ttttcatgtt ttatgagtag tgtgacttgt gaccgaggag ttggcaggag
    11701 agcaccggtt accaaataga taggcccgtt atggtctatc tattttttta tccagaaaac
    11761 gctgggataa aagaaaatca cttgttttgt tgaaaaaatg ggtatactgg gataaactga
    11821 aatcagcaaa ctatatttag gagtgacgca gttggacgca cgttatgcaa ttattttggc
    11881 ggcagggaaa ggtacccgca tgaagtcaaa attatataaa gttttacatc ccgtttctgg
    11941 taaacctatg gtagaacata tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat
    12001 tacgatcgtt ggacacggag cagaacaagt taaagcacaa ctaggagaac gcagtaaata
    12061 tgcgttacaa gcagaacaat tgggaacagg acatgctgtt ttgcaagctg cttctttctt
    12121 ggaaggaaaa aaaggaacaa cgttagttat cagtggcgat acaccattat tgactactga
    12181 aacgctgaac aatctttttg aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac
    12241 ggcacaagca gaaaacccaa caggatatgg acgaatcatc cgtgaccata ttgggatcgt
    12301 agaaaaaatc gtggaacaaa aagatgcaac accagaagaa gctcttgtac aagaaatcaa
    12361 tacaggaact tactgttttg acaacgaagc tttgttcgat gcgttaagca aagttggaac
    12421 aaacaatgct caaggtgaat actatctaac agatatcatc gaaatcttaa aagaagaagg
    12481 ccatacagtt gctgcttatc aaacggatga ttttgaagaa tcaatgggag tcaatgatcg
    12541 gatcgcgtta gctaaagcaa atgagatcat gagaaagcga attaaccaga tgcatatggt
    12601 caatggcgta agctttgtcg attcagcaac gacatacatc gatgcaggcg tagaaatcgg
    12661 tccagacaca ttgatcgaag caggtgtaca aatccaagga aatacagtca ttggttctga
    12721 ttgtgtcatc gggtcacatt caaaaatcgt agacagtagg attgaggatc acgtcgtcat
    12781 tgaaaactca gtcattgaaa gcagccatgt caagaaacat gcagatgtgg gaccttatgc
    12841 acatttacga ccaaaagctg aaattggcga aaacgtccat atcggaaact ttgtggaagt
    12901 gaaaaatgct caaatcggca aaggaactaa agtcggtcac ttgacttatg ttggtgatgc
    12961 tacgctggga gaagaaatca atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa
    13021 gaacaaacat catacgactg tcggcgacca cagctttatt ggttctagca cgaatatcat
    13081 cggacctgtc gaggtagcta aaaacagttc gattgctgcc gggtcaacaa ttacagacaa
    13141 tattccagaa tatgcgctgg caattgcgag agcaagacaa gtcaataaag aaggatatgc
    13201 aaaaaaactc ccttatctaa actaagactt ttgcaaaatc acttgattta tttaaaaaat
    13261 atgactacta tttattaggt agaattctag agttgtgaaa aagaaagtgg aggtccccat
    13321 gtcaaaacat tactttgacc caagattaaa aatttttgcg ttaaattcca atcgcccatt
    13381 agcagagaaa attgcagcag cagtcggcgt cgagttagga aaatcatctg ttactcaatt
    13441 tagtgatgga gaaattcaag taaacattga agaaagtatt cgtggatcgc atgtctatgt
    13501 tattcaatca acgagcagcc cagtcaatga taacttgatg gagttgctga tcatgattga
    13561 cgcactgaaa cgagcaagtg caaaaacaat caacgtagtc atgccttatt atggctatgc
    13621 gcgtcaagac cgtaaagcac gtgcacggga accaatcaca gcgaaattag tagcaaacat
    13681 gattcaaaaa gctggtgcaa caagaatgct gactttggac cttcatgcag tccaaatcca
    13741 aggtttcttt gatattccag tcgatcattt gatgggtgcg ccacttattg ccaattattt
    13801 cttagaaaaa ggaatcaaag gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt
    13861 cacacgggca cgtaaattag cggaattttt gaaatctccg attgcgatca tagacaaacg
    13921 ccgtccaaaa gcaaatgttg cagaagtaat gaatatcatt ggacaagtca aaggcaaaac
    13981 ttgtgtactg atcgatgata tgatcgatac agcaggtacg atcacattgg ctgccaatgc
    14041 tttgaaagaa gctggtgcaa ctagtgtgta tgcttcatgt acacatccag tattatccgg
    14101 ccctgcttta caaagaatag aagattcagc tatcgagcgt ctagttgtta cagactcgat
    14161 ctatcttcca gaagatcgta aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat
    14221 gggagatgca atcaaacgta tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa
    14281 aaataaataa taaataaact cacaaagggg agtccaattg ggctgagatt gaatctattt
    14341 ctaaaccctt cgtacctgta tcggttatgc gagcgtagga attgtgaata aacagctgtt
    14401 tgactatttt cgaaagctgc ttcctccttt gtgacttttt tattcaaagg aggaagtttt
    14461 tttatgggaa aaaatagagt ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg
    14521 ttgtcgttga ttccgatcca gatcggcagc agtttttcta tttcattagg gcagattcca
    14581 ttgactcttt ttgcgttaag aagagggaca aaggcaggga tgctggcagg tttcatttgg
    14641 ggcatcctac attttccgtt gggacaagtt tattatttaa gtgtcgttca agtgttgatt
    14701 gagtacccga ttgcctatac ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa
    14761 aaaggctatg tagacgataa tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg
    14821 attggcgcac tttccagata tttttggcat tttattgcag gagtggtttt ctggggagcg
    14881 tatgcactat gggggatgaa ttcttggctc ttttctttcg taatgaatgg tgccagcggg
    14941 ttgagtacag ctattgtaac gatcatcgtt ttattaggga tccaacaaac gactccttcg
    15001 atttttgtgc cagataaacg acaggagctg taaataaaaa agaagcaaga aacttgagac
    15061 agaatcatgt cctagtttct tgtttccttt ttattttttg ataagaaata gatctacttc
    15121 aatggcagat aagaaagatc ggcatcttct accaattgcc agtgtcgatc ggtgacagag
    15181 tagattaaag tagcgatgat ttcctcttct cgataccggg tgccttttaa tttccaaata
    15241 taatcttcaa tcacatagat tgggatacga tatttaccat cgatcaaacc aacttgatca
    15301 aaagaaaaaa tgatcccttc ctgcatcaaa ttacgcattt ctttcaataa ttccacactg
    15361 attttttcaa caggtgtttc ttttttgaaa aaagattttt ttacattgat cttatctgac
    15421 aagatcgaac ttaatttact agcaaaaatc ctataaaatt catctaaata gcgataataa
    15481 atccgattca gctgatcatt aagatcgaag tatacaaaat tattctgtag tttataaaaa
    15541 aacggcgaat gaagatgtgt tttcatatga ccaaaatata ataattctga aatttctaaa
    15601 ggtgtcaatt ctttcagcat cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat
    15661 ccacgcctgt tacgagaaga ggaaaagaaa cgctgaatat tttctgtacc acggattact
    15721 ttcagccctg tatgggattc atattctcca gcagaagcag atggatcaag caagagcaag
    15781 ttttgaggaa aatgcacgat actgtttgca aagtcagtag cagtcagccc ttttgttaaa
    15841 acagcattac ttgtggtatc aatatgtaca taaactgact cagtcatcat gttcctcctc
    15901 ttatttcctc ttaatctctt ttattttaca acatttgcat taaaaaagct tgtttctttt
    15961 attttaaaat tccattacaa aatcacgttt tcattgaaaa cactctcatt ttcaattaat
    16021 ttttgtg
//
LOCUS       DAJSMI010000057        15813 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJSMI010000057 DAJSMI010000000
VERSION     DAJSMI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.057"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(48..350)
                     /locus_tag="ON430_001396"
     CDS             complement(48..350)
                     /locus_tag="ON430_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219321.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(570..936)
                     /gene="ssrA"
                     /locus_tag="ON430_001397"
     tmRNA           complement(570..936)
                     /gene="ssrA"
                     /locus_tag="ON430_001397"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1107..1880)
                     /locus_tag="ON430_001398"
     CDS             complement(1107..1880)
                     /locus_tag="ON430_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3219322.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2024..2374
                     /locus_tag="ON430_001399"
     CDS             2024..2374
                     /locus_tag="ON430_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCR3219323.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2355..3071
                     /locus_tag="ON430_001400"
     CDS             2355..3071
                     /locus_tag="ON430_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3219324.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3071..4519
                     /locus_tag="ON430_001401"
     CDS             3071..4519
                     /locus_tag="ON430_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3219325.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4589..5098)
                     /locus_tag="ON430_001402"
     CDS             complement(4589..5098)
                     /locus_tag="ON430_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219326.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5088..5813)
                     /locus_tag="ON430_001403"
     CDS             complement(5088..5813)
                     /locus_tag="ON430_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219327.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6036..8156)
                     /locus_tag="ON430_001404"
     CDS             complement(6036..8156)
                     /locus_tag="ON430_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3219328.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8386..9564
                     /locus_tag="ON430_001405"
     CDS             8386..9564
                     /locus_tag="ON430_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCR3219329.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9580..10338
                     /gene="aroD"
                     /locus_tag="ON430_001406"
     CDS             9580..10338
                     /gene="aroD"
                     /locus_tag="ON430_001406"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR3219330.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10361..10846
                     /gene="ybaK"
                     /locus_tag="ON430_001407"
     CDS             10361..10846
                     /gene="ybaK"
                     /locus_tag="ON430_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCR3219331.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10917..12170)
                     /locus_tag="ON430_001408"
     CDS             complement(10917..12170)
                     /locus_tag="ON430_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3219332.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12287..13636
                     /gene="gdhA"
                     /locus_tag="ON430_001409"
     CDS             12287..13636
                     /gene="gdhA"
                     /locus_tag="ON430_001409"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR3219333.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13748..15094
                     /locus_tag="ON430_001410"
     CDS             13748..15094
                     /locus_tag="ON430_001410"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR3219334.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15402..15788)
                     /locus_tag="ON430_001411"
     CDS             complement(15402..15788)
                     /locus_tag="ON430_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3219335.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5169 a         2829 c         3179 g         4636 t
ORIGIN      
        1 tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta atctttatac
       61 ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc gctccgtgcg
      121 tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat tctggttttt
      181 gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa taagctatct
      241 tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt gacaaatcta
      301 ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat aatgttcacc
      361 tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat tgataatatg
      421 gatggaacat atatattaaa aagtgttctt tttgaatgct attttgaatg ccattttcat
      481 tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac ccttataaaa taaggattta
      541 agcatctagc gaaatctgct gaaatctcac aatggagacg gcgggagtcg aacccgcgtc
      601 caaacacatc gccacttaaa aatctacgtt catagtcaac tcatttaagg attcacttta
      661 cagcttgccg agtgacaggc attctgtatc gctagtctga tgttctcttt ttcaacttac
      721 agacggaaag ttgaaacgta tcccactaaa gttaggaccc ttactcgagc acatgggcga
      781 tgccgagagg atctacgcta actgttttta ggcagctaaa gcgtaagagt tgttttcgtt
      841 tttagcagtt atatttaact gtaacgtttt tacgtagacg taacctacga aacgcaattc
      901 aagctcgact gtgcctgtcg aatccgtaac gtccccgtta gctaacaagg gttacgtgta
      961 tttttcatac aaaattagta tagcatatat tgggataaat tcaataaaaa agcgaatcat
     1021 atcccattat ttatataata actgcccctt tcttaagaaa aaataagcga ttttcttaag
     1081 aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa aatacttcaa tgcgtggaca
     1141 attccgcctt ctgtattttt ctttgtgata aaggtagaaa gttcttttaa tccgtcatag
     1201 gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca aggcaaaatc attcggacca
     1261 tcgccaaatc caaaagtagg aactttttcc agctgaagtt ctttttgcag acgtttgaca
     1321 cctgcccctt ttgagattcc tttcttcact gtatcgatcg agtaaggacc attccgataa
     1381 aaagttagct caggaaaatg ttcgtgatag tattcgtcac ctgattgaga taagatcagc
     1441 aacatattta ctgtgttttc tttaaagcct aaaggatcaa tttccggaac atctgaatga
     1501 atgaagtcgt aagcattgat cattgtttgg ctatgaccgg tacaccagat tttctgatcg
     1561 ttatagtaag aaagttcatg accgttcaac tgtacatgat catacatttt ttgacattct
     1621 tctgaagaaa atgtttgaga atagatttct tcgccgtcta ctcgaatgaa agaaccattc
     1681 atggtgaccg cagaagtaat gcccgcatct tccatgatag agtggatttc tgcttctgta
     1741 cgtcctgtag cgatgacggg gagaacattg ttttctttta atgcgttcat agcttttgcc
     1801 acttcgggtg tgatttttga tttcgcatct agtaatgttc catcaagatc aaagaaagtg
     1861 attgcacgat aatttgacac aaataaaact ccttttttat tggaataagt ttatcttaac
     1921 aaagaatgaa caaaagacaa acctttcgtg aagaattcct gaacatattg gttcagtcaa
     1981 caatttagag tatactataa gaagagaatt taggagggaa agaatgattt ttttcagtat
     2041 tggtatctta ttcttaataa ttggcagtct ttttcggatt ctgccctcaa aaggaaatct
     2101 gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca gacgctcatt ggcggctggc
     2161 tcaaaagaca agcggaaact ggttttttct tatgggctta ctgatggcat tgatcggcta
     2221 ttatttaaag acatctgggc atacgaatta ttttctgatt gaaatgcttc tgctagtatt
     2281 tccaattatg cctattttta ttatgacaga aaagaaatta cagaaatatg acctggaaac
     2341 tggagggaac gataatgaat attttaatga ttgaagataa tcaatctgtt tctgaaatga
     2401 tgcagatgtt tttcttaaat gaaggttggg aagcaacttt caagtatgat ggaaaagaag
     2461 ggctcgatgc ctttttagaa aacccacaac attgggatat gattacacta gacctgaatt
     2521 tgcccacact ggacggtatg ctagtaggaa gagagatccg caaagtttca agtactgtgc
     2581 cgattatcat gctgacagct agagattctg aaagcgatca ggtgatcggc ttagagatgg
     2641 gggcagatga ttatgtcaca aaacctttca gtcctctgac gcttattgct cgaatgaaag
     2701 ctttgcatcg ccgtagtgag ttggcagaag caagagaaga agcagtagtt tcagatgatc
     2761 attttgatat cgagactagt catttcaaga tgaatacgaa aacacgagaa gcgtatctgg
     2821 acaaccggcc aattgaagga ctgacgccta aagagttcga tctattgttt actttagcaa
     2881 aaaaaccacg tcaagtattc tctcgggagc agttgcttga attagtctgg gattatcagt
     2941 actttggtga tgaacggaca gtggacgccc atatcaagaa attacgacaa aaaatcgaaa
     3001 aggtcggacc acaagtcatc caaactgttt ggggagtcgg ctacaaattc gatgattctg
     3061 gtgttgccta atgcgttatt tgtatcagca actcttagct ttctgcggaa tggtcgcgtt
     3121 gatcttattg atcgttggag tttcttttac acaactgaca aaacaaacga ttgaagaaaa
     3181 caattaccag caactgtttg gatatgctga atcagttgaa aagacaacgc aaacctatgc
     3241 cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa aatgcattgc tgctgacaga
     3301 gcaagtactg acagaacaaa atgtgaactt tatatttatc gataaaaacg aacgtgtcat
     3361 ttatccgact aatacagctt tactgaattt taccttaaca gctgatcaat ggaaaaatct
     3421 gaggaatgga cgtcaagaaa aattcacatc gaataaaaat attttaggac aagcacaggc
     3481 gacctcttac gcacttgtgc catttaattt gaatcatgaa ttctatggag cacttgtagt
     3541 tagtcaacct gcgcgaaata tcgataacag cgttcgtcca attacactga acctgttcaa
     3601 aggatttatt ttttccagta tcgttgcagt tattgccagc tatgcttttg cagcattcca
     3661 agttaagcgt atcaatcggt taaggaatgc gacgaaagaa gtgacgaatg gtaattttga
     3721 tgtacagctc cccgtacatg acaaagatga atttgatgag ttagcagatg atttcaataa
     3781 aatgacgaat tcattgaaag aatctcaagc agaaattgaa gaacaagaaa atcgtcgaag
     3841 acaatttatg gcagatgctt ctcacgagat gagaacacct cttacgacaa tcaacggact
     3901 tctggaaggg ttagaataca atgcaatccc tgaaaatcag cgagaaaacg cgattaaatt
     3961 gatgaaaaat gaaacagagc gtctgatccg tttggtgaat gaaaacttgg attatgaaaa
     4021 aatccgtacg aatcaaatca gtatggtcat taaaaaattc aatggaacag aaacattgaa
     4081 aaatattgtt gcacagttag aagctaaagc agaagcagca ggagatacat tgacattgaa
     4141 agccgttgat gatattgatg tttacgctga ttatgaccga tttgtccaaa tcatggtcaa
     4201 tattattcaa aacgcgatac agtttactga aaacggccaa atcacgatta ctttagaaaa
     4261 aggctatctt gaaacgatca tcacgattga agatacggga attggaatgt ctgaacaaca
     4321 aatgaaaagt atctgggatc gttattataa agtagatcct tcaagaaaaa atacaaaata
     4381 tggtgaatca ggattaggtt taccaatcgt gcagcaactt gtacgcttgc ataaaggaaa
     4441 attagaagta gaaagcgaac taggaaaagg aacaaaattc gctgttcgtt tgcctgacgt
     4501 agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat catgaatcga tcttctatac
     4561 cgcttgtttt tactataaaa tgattgattt agcccgcggg ctctaaacga tcaatataag
     4621 gactttgagc aatagtagca tcatgagtaa agagtaagat accttgattt tcttcatgag
     4681 ctaaataaga aagcaagtgg agtagctctt ggatctccaa tacactaagt tcatcaaatg
     4741 tatcttcaat aacaatattg tttcgcttgg aaagcaaggc atgaagtaat tgcagcttta
     4801 ctttttcaaa agacgtcaac tcagctgccg cacaattaag caaggaagga gagatatcaa
     4861 gtttttcttg ataggccaaa cgatcttgtt tgatttcttt ttccggtaca tccaacagga
     4921 catggtcttt aagattcaga taaggaaaca tcggccaatt tttttggatg atcgcaaaat
     4981 tatccagttt tgcaacaaga acctcttttt tcaagtgctc caatatttta tgctgttttt
     5041 cgagaggaca caaaataacg gtcaagtgtt cagcatgaat caaatattca ggtttcatta
     5101 cgtccaggcc tcctactttg caaatgatgg ataaaaaaag tgaaagggaa tagactaaga
     5161 atgagacagc atccactgag cccaataaga gtttgggcaa atcccttaag tatatctgtg
     5221 aaaaaaagat cattttggaa atcgatggta aaaaaagtct gttgatccat cggaattgta
     5281 agtccgctta ccgaagtcaa gatatcacgg atatctggca gttcagtagt ctttaagaca
     5341 agaaaattga tcctttgaag aagcgcttca taaactgact ggaagcttac gatccacaat
     5401 aaaaccaaga tacaacaaag aaagagagga aagaacagct gccagaaaat aaaaaggaat
     5461 aatttggttt tagaaagacc agtcaggtgc caattgatca attcctgctt gttttgcctg
     5521 actgaatagt aaaagaaaaa gataaaaagg aaggcaaata gaaaaaataa actgaatatg
     5581 ccttttatat aaaatcggct agctaattct aaagccggaa gaagctctgt agatgcagaa
     5641 gcgccaataa atgaaagccg atcttggact tgattggcta aagccgattg caaatggtaa
     5701 agagtcaggc atgctatcaa taaaaaagca gaaaagcttg aaaataaaca atagagtatt
     5761 gtctgctttt tgtaataaat taatcgagca gtagcatagc ggattatttt catacttttc
     5821 acctctataa aaagtataga aaatgaatat gaataaagta tgaaattcct ttagtgaaat
     5881 cacgaattaa aaccagaaaa tattttgtaa aaagcaagag gttgtgaaga agctgcccta
     5941 ttctattacc gtagatgcaa gcttttgaac actgtttatt cgaaaagagg agcagcgaca
     6001 ataattttgt cgctgctcct tttaaattta ttttatcatt cggaagaact tgtggtcgtt
     6061 ccgtgaagtt cttggtacgt ttgcgtttta taaagatcaa cagttgattg tccgttgtgt
     6121 tcactaaaga cactagttga cttgtcacca agagattttt cagcttcttt taatttctct
     6181 aagccattct tgtagtcata atcactggta tcaattgggt ctaatccact attcgtatag
     6241 aaccgaagaa gatcaccatt attgatttgg tcagaaacag ctaactgttt atcaactttt
     6301 tctttcagtg aatcaatttc attctgtact tcctcagtag gttcagtaat agcttcacca
     6361 gtttcgttac tatagatcgt accaccgtag taagtatatt ttggtgtaac aaaatcacca
     6421 tcacggaaag aaacaagctg ttcatgatct ttcgagaata gatcttgtcc taactgaata
     6481 taattttttg tatctacacc tagtaagtga agtaatgtag gcaaggcatc aacttgtccg
     6541 ccgtaagtgt gattgattcc gccctgtgtt tgtccaggga tatggatcat ataagggaca
     6601 cgctgcattt gtgcattgtc gtagtttgtc caagtagaac ttgtttttcc aactaattca
     6661 gctagatttt tatttcttga attcgagaca ccgtaatggt caccgtaaag gacgatgact
     6721 gaattatcat acaagccgct ttctttcaaa taattgaaga attcttgtac ggcggtatct
     6781 aaataattgg ctgtcgcaaa gtaaccgttg atcgtttcat catttgtatt ggctaacggg
     6841 aatcctgctt cgtcattggt gaattttgaa taaggataat ggttagatac agcaatgaat
     6901 tttgaataaa atggttgttg cagacgttcc aagtattgta cggattgttg gaaaaatggt
     6961 ttatcatgca acccgtattg gaacgaattg tcatcattaa catcataata agaagaatca
     7021 aagaaataat catatcctaa attcttgtat gtctcgtttc gattccagaa tgtaccagcg
     7081 ttcccatgga ataccgcact aatgtaacct tgtgtttgac ttaaaatatc aggtgcagct
     7141 tggaaagtat tttttccgcc taattgtgtg aacaaggagc cttgattcaa gccaaacaat
     7201 gaattttcca tcagcgtttc cgcatcgctt gtttttcctg cttttacttg atggaaaaag
     7261 ttgtcaaaac tgaacgtgct gttgctgtgg taaagactat tgatgaaagg tgtaacttca
     7321 tgctggagac cattctcatc tgtcaattta taatcgatca ggaattgctg gaagctttct
     7381 aaatggatat aaatcacatt ttttcctcta gcgagcccga acaaatcgtc atttggtgct
     7441 gcataatgtt cttttacata attttctact tctttcatat cattgggact tgcttctgcc
     7501 cgcacttggt ttgtctggta agtttgaaca ccgtcataaa ccatgaaagc attcaagcca
     7561 agatatttga ccaaataatc acgagagaat gttcgagtca gcaattctgg acgatcagct
     7621 tccgccataa aaagatttcc tgagaaaatc ataactgcta atgtagagat cgcaaaagcc
     7681 attcttgctc ggatcgggcg agggtccaac ttgatttttt ttgcaatcag cagaacagtc
     7741 aagataataa aatcgatcca atagatcaca tcatatgggc gaaacagtcg aatggcactt
     7801 tctcccagac cactcgctac tttcccagca cctaaaatcg tgttgatcgt aatgaagtcc
     7861 gtgaattctc gataataagt gacattcgaa aaaacaagca atgtcaaaag aaaataaatg
     7921 accatcatcg tgatataagc aactctaggg cgtctgatat aaagagcaat tccaagtaat
     7981 aacaatgtag ttgcaatagg gttgataaac aagataaagt attgaatgcc actttcgatt
     8041 ccgagatgga aatcaactaa ataggcaaaa atcgttttca accaaagtaa aacgactagt
     8101 aaagaaaaaa atccgagcct cttattcaaa aaggcaggtg tttttataca tttcaagata
     8161 atcttccttt ctgcacttct taatcacaga agtgtcctga aaccatgtta cacagctcac
     8221 ttattctcat ttattttact agtatgacaa tatagtgtca attgaaacca gaagtttatg
     8281 ctgtttcatt aaggttttct ttaagcttca tgaaaaaatg agcagtaaga caagtcctat
     8341 gttttttgtt atacttggga agagaaagct aaagcaggtg aaggaatgaa agtaaaagta
     8401 aaaaaaagag caagcaatcg attgaaaaaa ggatatccac tgatccaaaa agaagacctt
     8461 gctcatcccc aaaaagaaac cacgcaatgg gtggaattag ttgattcgca aaaccaattt
     8521 atcgcaaaag gatatctagg aaagcagaat aagggaatcg gttgggtatt gagccagcag
     8581 aacatcccat ttgatcaatc tttttttgaa gaattttttg tgaagtcaaa agaaaaaaga
     8641 actttctttt tttcagatga ttctactacc gcttttcgta tattcaatgg agaaggagac
     8701 gggatcggcg gtctgattgt tgattattat gcaggttatg ctgttttttc ttggtataac
     8761 gaaacgttat atgcacatcg tgaattattg attcaagctt ttcgtgaaat ctacccggaa
     8821 atcataggag gctatgagaa aattcgattt gaatcagtaa atctgcctga gagccagttc
     8881 atttacggaa aagaggcacc ggaacccttg atcgtaaaag aaaacggagt aaaatttgcc
     8941 gtttatttaa atgaaggatt aatgactggg atctttttag accaaaaaga agtacgggga
     9001 tcattagcag aaggtttcgc ttctgggaaa acagttttga acatgtttag ttataccggc
     9061 gccttttctg ttgctgccgc aatgggaggg gcaatcgaaa caacaagtgt cgatctagca
     9121 aaacgcagtt taaaaaaaac acaggaacag tttgaagtga atggtttgga tgtcgatcag
     9181 caaaaaatca ttgttatgga cgtattcgag tatttcaaat acgccaaaag aaaacaattt
     9241 ttatatgaca tgattgtatt ggatccacca agttttgcca gaaataagaa aaaagtcttt
     9301 cgcgtagcaa aagattatgg ggaactggtc aaggatagtc tggatatttt agcagaagac
     9361 gggatactta ttgcgtctac aaatgctgcg aatgtctcga tagaacaatt ccaaacaatg
     9421 attgaagagg aattccatgc caaaaatgtt tcttttgaac aacttgcttt atatcgtctg
     9481 ccaaaagatt tccagacgac ggataccttt ttagaaggca attatctgaa agtgtttgtt
     9541 tatcaaatta aaaataacca ttagaaggag cgatgcagaa tggttcgggt aaagggattg
     9601 aatataggaa gcggtagacc aaagatttgt gcgcctctta tcggggccgt ccgagaggaa
     9661 gtattacgtg aagcagttct tgcacgtgaa gcaggggccg atttagtcga atggcgagtc
     9721 gatcattatc gtgaagtttt ttcttttgat gaagtagcag aaacattggt tcttatccat
     9781 gagatgcttg atggactgcc gttattattt acgtttcgta ccttagctga aggcggagaa
     9841 caagacatat cgatccgtca gtatcgcgac ctttatgaac atgtgataca cacaggatta
     9901 gtagatatgg tcgatatcga gttgttcaaa gtagaaagtc taggaaaaag actgatagag
     9961 gaaatcaagt ctttaaacat tcctttaatt atcagtagtc atgattttaa agaaacacca
    10021 gcagaccctg tcttgcttta ccgattgaat gttatggaac attttggagc cgatatcgga
    10081 aaactggcag taactcccaa taatgaacga gatgttttgc ggttgatgga gttgactaga
    10141 cgggcaaatg cttttgtctc catgcctatt attacgatgt ccatgggaaa tcttggtaaa
    10201 atttctcgtc tggttggcgg aacgacgggt tccgttatga catttggttc cttaagcagc
    10261 gattctactt ctgctccagg acaattaccg gtcaaagcat tgaagcaaat tattcaatta
    10321 ttaaatgaac cgttgtagaa caagtttgag gtgatatttt ttggcaaaga aaaaaatagt
    10381 aaaaacgaat gcaatccgta tggtcgaaca aaaaaagatt ccctatacag agcacgaata
    10441 tgagtgggac gaaagccacc taagtgcgtc atcggtcgct gaacagcttc cagaaagcca
    10501 atctcgtata tttaaaacac tggttgctgt gggcaatgtg acggggcctc ttgttgctgt
    10561 gataccggga gaagccgaat tgaatctaaa aaaattagca aaagtttcag ggaataaaaa
    10621 agtagaaatg ctccatttaa aagatttgga agcaacgacc ggatacatac gtggtggctg
    10681 ctcgcctata ggtatgaaaa aactatttcc tacttattta gatcaaattg cagaaagcta
    10741 tgaacaaatc attgtctcgg ctggcagaag agggctacag atggagttag cacctcaaga
    10801 tatctgtgca ttaacgagcg gacaatttgc agatataaaa caataaatca tacgtataaa
    10861 agaaagaagg aggccgggat gttgtttgtc cagtctcctt ctttcttttt tatacattat
    10921 gctctttgcc cggcattcat tcctgctaca tgccctgtag cgaaagccgc tgtgatatta
    10981 aagccgccag tataaccatt gacatccagt aattctcccg caaaatacag accatcaatg
    11041 attttactct ccaatgtttt aggataaatt tcctttagag aaacccctcc gccagttaca
    11101 aatgatttct caataggaaa agttttgttg attgatagct taaactcttt gcatagttga
    11161 acaaaatctt gaatttgttt ttgagaggct tgttgtcctg tcagatgatc catttcaagc
    11221 cgctcaagaa agaaaactaa caatcgctca ggcaatatac catgccaagc attgaccaag
    11281 ttctttttgg ttgctttact tttttctgtc aatatatgaa taagctcttg tttggtttgt
    11341 gtagggaaac aatccagaga aagtgtcact ggttctcccg ttttctctaa ttctttattg
    11401 acaaaacttg aacaacgcag tgcagccgga ccagaaatac cgaaatgggt gaaaagtaaa
    11461 tccatcgtat gttcagtcaa tacacgacct tttttattta aaacgcgtaa agtgatgtct
    11521 tgtaaagaaa gcccttgaag tgttttctcc tgaatatacg gttcatccga tatcaagggg
    11581 gactctgtcg cataaagagg cgtcaccgtg tgacccactc gcttcacgat cttgtaccca
    11641 tctccagtag aaccagtaga tggataagtc cgtccaccag ttgttaagat aatggcagga
    11701 gcatggaact cttcgaattc cgtgcgtata ccgtaaattt tatgttcttt gtggatcaat
    11761 ttctctactc gagtagagaa tagaagcgtc acatctagtt ctttcagacg attgagcaat
    11821 gcttcgataa tcgtttttga tttatttgtt acaggaaaca tccgtccatg atcctcttct
    11881 tttaaatgta cgccttgtga ttcaaagaag ttcatgatat cgaaattatt ccattgcgca
    11941 aacgtactat ataaaaactt tccatttcca ggaatatgtg caatcaaatc atcaacagga
    12001 cgattgttcg taacattgca ccgaccacca cctgtcatca gtagtttttt tcctgctttt
    12061 ttgttttttt cgatcaagag tacgcgtgcg ccttgttcag ctgcactgat cgctgccatc
    12121 atcccgcttg ttccagcgcc gactacgatc acatcataca gtttttccat tccctcacct
    12181 caaggaaagt gtagcatatc tgaagaaaga aaacagaaaa aaatgttttg agccaaaaga
    12241 gaaaatgagc taaaatgagg gaagataagc aaaggagcgt ggaacgatga caaacgcaac
    12301 agaatatgtt caagcaattc aagaaaagct acatcaaaag gatcaagggc aagaagaatt
    12361 tttacaagca atcgatgaat tcatgcccac ggttatgtct tttttggaca ctcatcccga
    12421 atatatcgaa aagaatatct tagaactttt aacggaacca gaaagagtta ttcaatttcg
    12481 agtgccttgg caagatgatg aaggaaactg gcgagtaaat agagggtacc gtatccaata
    12541 caactcagct atcggccctt ataaaggtgg attgcgcttc catcctagtg tcaatctaag
    12601 tatcttgaaa tttttggctt ttgaacagat tttcaagaat agtttaaccg gtctgccaat
    12661 cggtggagga aaaggcggaa gtgacttcga tcctaaagga aaatctgata atgagatcat
    12721 gcgtttttgt caaagtttca tgctggaatt gtcaaaacat atcggcccat cgattgatgt
    12781 tcctgcggga gatatcggtg taggagctag agaaatcggc tatttgttcg gcgaatataa
    12841 acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa cctttggatt tttggggaag
    12901 taagatacgt acggaagcta ctggttatgg attagtgtat tatgtcaaac atctattgaa
    12961 cgaagaaaaa gattcattta atcaaaaaac agtttttgtt tccggaagcg ggaatgtagc
    13021 catttatgca atcgaaaaag tgcaagagct aggcgggaaa gttgttacat gttcagattc
    13081 tagcgggtac atctatgatc cagaaggaat agatgtgaaa ctattgaaag aagtcaaaga
    13141 aatcaataga caacgtttga cggaatacgc aaaccaacgt ccttctgcta tttatcatgc
    13201 tggagaatct gtttggacat tgaaagaaaa agcggatatc gctttgccat gtgctactca
    13261 aaatgaaata gacgaaagct tggccgaaat cttagtagaa aatgggatca aaatcgtagc
    13321 agaaggcgct aatatgccat gtacagtaaa agctgtttct gttcttcatg aagcaggcgt
    13381 gtggtattgt ccaggaaaag ctgctaatgc tggaggagta gcagtatctg ctttagaaat
    13441 gagtcagaac gtccagcgat tgacttggga aagagagcaa gtggatgagc agctggatca
    13501 aatcatggaa acgatttacc atacgtgtcg ccaaacagca gccgagtacg gcgacgaaaa
    13561 aaatctgctt cttggagcaa atgttgctgg atttgaaaaa gttgccaaag caatggcagc
    13621 agaaggttta gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt ttgtttattt
    13681 gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt agagtaaacc tataaggagg
    13741 agaacaaatg gcacacattc attttgacta ttcaaaagta gcaccgtttg tcaacgaaca
    13801 agagttaggg tacatgcaaa gccaagtaac agcagcccac aacgaattac gggaaggtac
    13861 tggcgcagga agcgacttcc gcggctggat cgatttacca acgaactatg acaaagaaga
    13921 atttgcacgt atcaaagaag cagctaaaaa aatccaatct gattctgaag tattagttgt
    13981 tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg atcgacttct tgaatcacac
    14041 attcttcaat ttattagata gcaacacaag aaaagcacca caaatcttct ttgccggaaa
    14101 ttcaatcagc tcaacatata ttgctgactt gatcgaagta atcggtgatc gcgacttttc
    14161 agtaaacgtt atttcaaaat ctggaacaac tactgaacca gcgattgcgt tccgtgtatt
    14221 caaagagctt cttgttaaga aatatggaca agaagaagca aacaaacgta tctacgctac
    14281 aacagataaa gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt gggaaacttt
    14341 cgtgattcct gatgacatcg gcggacgttt ctctgtatta actgctgttg gtttgttacc
    14401 aatcgctgtt agtggtgcag atatcgatgc attgatgcaa ggtgccgctg atgcaagcaa
    14461 agcatactca agcgacaaac tagaagaaaa cgaagcttac caatacgcag caatgcgcaa
    14521 cattctttac cgtaaaggca aagtaactga gttgttgatc aactatgaac caggaatgca
    14581 atacttctca gaatggtgga aacaattgta tggtgaatct gaaggaaaag accaaaaagg
    14641 catctatcct tcaagcgcaa acttctcaac tgatttgcac tctttaggac aattcatcca
    14701 agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc gtaaatcaat
    14761 cacaattcct gaacaagcag aagatttaga cggtctaggc tacttgcaag gaaaagaagt
    14821 ggacttcgta aatacaaaag catttgaagg aacattgctt gctcatacag acggtgacgt
    14881 gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat acattgggtt atacaatgta
    14941 cttctttgaa attgctgtag gtatttcagg ttacttgaat ggaatcaacc cattcgacca
    15001 accaggtgta gaagcttaca agaaaaacat gttcgcactt ctaggcaaac caggctttga
    15061 agatcttgct aaagaattga acgaacgcct gtaagataag ggtttttcgc tagatttaaa
    15121 taatcagtga tagaaacatg ttcgctttaa aatgaaaact ggtacccatt taggtactca
    15181 aacatactgc atcgtttatt cagaataagg ggctgtgaca agacgtttgt cacagtccct
    15241 tgtttgtata gaaatcgatt gattgccttg ctctttagtt tgttttagga ctttaagaac
    15301 agtgaggctg acttcgaagg ttaattctgt ttttttgttt tctagaatca gtaagcaaaa
    15361 aagcactcac ttcgagtgct tttttgcgat atattcttag cttaagatag gcctaatttt
    15421 tcttttacag atgagggtac cttatcgctg gtaattactt ttttctctat gatccgcttt
    15481 tcgcttgctt tcaactgcaa gatcgttcct tcggggtaag cagaaccatt ggcagagtca
    15541 ccagaagtaa tgacatcgaa atctaactgt cttttttcac cgttttcagc ataagcatct
    15601 acgttgtatt taaaacctct tccaattact tccccattat ctgctttgat atcagtttct
    15661 gcaggcattg gagctttgac taccgcataa taatctttac ctacataagt agactgatag
    15721 tagtcccaac ctttccatcc tataaaagca atcaatatga taacgatcaa ccctaaaatt
    15781 tttttcatga aagatctcct tttttatttt tat
//
LOCUS       DAJSMI010000058        15564 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJSMI010000058 DAJSMI010000000
VERSION     DAJSMI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.058"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(251..1462)
                     /locus_tag="ON430_001412"
     CDS             complement(251..1462)
                     /locus_tag="ON430_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3219336.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
     gene            complement(1580..2215)
                     /locus_tag="ON430_001413"
     CDS             complement(1580..2215)
                     /locus_tag="ON430_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCR3219337.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2298..2720)
                     /locus_tag="ON430_001414"
     CDS             complement(2298..2720)
                     /locus_tag="ON430_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR3219338.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(2738..3058)
                     /locus_tag="ON430_001415"
     CDS             complement(2738..3058)
                     /locus_tag="ON430_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3219339.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3351..3542
                     /locus_tag="ON430_001416"
     CDS             3351..3542
                     /locus_tag="ON430_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219340.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            3556..3807
                     /locus_tag="ON430_001417"
     CDS             3556..3807
                     /locus_tag="ON430_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3219341.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            3783..3959
                     /locus_tag="ON430_001418"
     CDS             3783..3959
                     /locus_tag="ON430_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219342.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGNRQALIGTIVYMVWIFIGLDE
                     AEYRAKKRHERQQA"
     gene            4019..4336
                     /locus_tag="ON430_001419"
     CDS             4019..4336
                     /locus_tag="ON430_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219343.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            4269..4748
                     /locus_tag="ON430_001420"
     CDS             4269..4748
                     /locus_tag="ON430_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HCR3219344.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPMPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            4769..5509
                     /locus_tag="ON430_001421"
     CDS             4769..5509
                     /locus_tag="ON430_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3219345.1"
                     /translation="MVKKIKAEDLSVEKGTYMIYANPGMGKTYSLGFLPGKSLVLDVD
                     GSSSTLAKHPNKENIEVWELDSSDIWQEWLDTISDLVANKSSYEKQFDNICVDNISEL
                     FKAQLEDLGKKGKNSGVPSQADYQRTDFMNLRGLRALNNLDCRIVLTAWETTDTYTEP
                     NGQFFTRSMPDIRSKILNNFLGLCDVVGRLVIKKDDDGNETRGLILKPTSNVYAKNRL
                     DERSGCLVEQLVVRAGGEPNVSTPTVSD"
     gene            5481..7004
                     /locus_tag="ON430_001422"
     CDS             5481..7004
                     /locus_tag="ON430_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3219346.1"
                     /translation="MYQLRPYQIKLVQEARKHLSQGKKGVLIQSPPGSGKSVVIAEIV
                     RLATRKGGTVLFLAHRRELLDNIRETLEQNEVDLSKVIILSAVMAKNRLNKLPKLSLI
                     ITDEGHHGKAKTYMDIYNHFKEIPRLGFTATPYRLNGEGFTDIYEEMVEGPSIQWLID
                     HHNLAPYRWYSIPLIDRSKVDFKNMSREAESSARLFESDATIQGDIVKNYKKYADGQQ
                     AIVYAPTIQVSKMIVKWFNDNGIYAVHADGKTPTKERDDIMANFKSKKITILSNVDLI
                     SEGFNVPDVGVIILCRPTQSIVLHLQQSMRGMRYRENKTSIVLDHVGNGANLGLPTDE
                     FEWSLSARKKKSNGSSSEAPRMTCSNCGQQFLLKSLLKIENKPHCPFCLQEIVSEEKE
                     NSVTFDEAVQMVELNAENAKLARLSRKKFSKKQSLELNYAIAKAKVSFEGKGNPLFKM
                     FGSLTAYQGQHYSIDQLEELSLIKDVSMESILRAYKWALEKLNSKQEEPEWVKNTFY"
     gene            7022..7528
                     /locus_tag="ON430_001423"
     CDS             7022..7528
                     /locus_tag="ON430_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HCR3219347.1"
                     /translation="MTAFKVDYNEAQDFGAVPDGDYEVVIFNVTEDASKGGTEFINFD
                     MVIRNDIKQPRQNSHLFHRVWKSKETGKYNRGMIMSLAKSFGLPDGKEYQSFENFLED
                     FAMRPAKVKVKNEQSEYNGKTYDNTNIKKFETTKFPELQHQWKKNNAEKSVNSSPAFD
                     ISDNDLPF"
     gene            7528..9864
                     /locus_tag="ON430_001424"
     CDS             7528..9864
                     /locus_tag="ON430_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR3219348.1"
                     /translation="MNNYERIPLELRELNQWGIYKRSWNEQRGKWSKKPYDPFTGELG
                     SSTNESKWSDFKTALSAVSTFNADGLAFYFKPPYIGIDLDDIGDDLERYLQGDVENNL
                     VYVFMNSTKTYSEISMSGKGIHIIGKAAIPGERRRKGNVEMYTEGRFFAITGNFFGNN
                     EEINEIPEIQMNFLYKRYLENETVIKQDFSKSNWSDGNDLSVNEIIQTAVNSSTGNRF
                     RLFMDGGWEKIYDSQSEADLAFANDLAFWTAGDFQKMDEIFRMSSLFRDKYDQKRGKT
                     TYGIGLLNKAISENTNHYTGKKTADDYFLSIPGITVDETKPTKFYSYDDTGNAERYLD
                     LFGSFTKYSYVNKCWYFYNGKNWEQDNIGAVRKWVDQTIEIFKNEPVSIPKDATEDEE
                     KAYIEAKEKHLRRSRNNAGKEAMTRELKHQVAILPDEFDSDDMLFNAQNGYLDLSNGI
                     LYEHDISKMFTRISNAEYTDKSDCPRWKLFLEQIFDNNTELIRYIQKAVGYSMSASIR
                     EQVMFILFGNGRNGKSVFLDIISEIMGSYAMGMQASTLMVKQGGSSGHNEDIARLNGA
                     RLVTSSEPNEGVRMDEGLIKQLTGGDKVSASYKGGHMFDYKPKYKIWLATNHKPIIRG
                     NDDGIWRRLPLIPFTVQIPLDKVDKNLKEKLMRELPGIFNWAVEGCLMWQREGLNPPA
                     DIQRATMEYRKEMDIIGAFIDECCETGPGYSIGATDLFKAYDKWARDMNEHPFSQTQF
                     GKKAADRFEKSKSKGKVVYRGIDLKKEFREFNVLVPGL"
     gene            10179..10496
                     /locus_tag="ON430_001425"
     CDS             10179..10496
                     /locus_tag="ON430_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCR3219349.1"
                     /translation="MTAEIEIQNAIRRELPKYGHFVYRGNVGKVKTIDGRWFDTGLPK
                     GWPDLFGWTKEGKFFAIEVKNEKGKLRPDQVKFGDFLQKQPVLYGVARSVEEALKIVE
                     ESS"
     gene            10493..10654
                     /locus_tag="ON430_001426"
     CDS             10493..10654
                     /locus_tag="ON430_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3219350.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            10657..10956
                     /locus_tag="ON430_001427"
     CDS             10657..10956
                     /locus_tag="ON430_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219351.1"
                     /translation="MDKKATMKRIVELTKSESWQEDKEIVAEVQRIGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            10956..11273
                     /locus_tag="ON430_001428"
     CDS             10956..11273
                     /locus_tag="ON430_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCR3219352.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYKE
                     DSK"
     gene            11270..11686
                     /locus_tag="ON430_001429"
     CDS             11270..11686
                     /locus_tag="ON430_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219353.1"
                     /translation="MIPKFRAWDKKTETIQEIESISFKEKKLVIDQKSVTWFNSDYIR
                     NFGEVELMQSTGLKDKNGVEIFEWDIVLVNVSNGFDHLVNEKTVVQESEFHSGLIYKS
                     LASGMEYRVFKHKKMDYEYEVIGNIYENSELLEEQR"
     gene            11683..12243
                     /locus_tag="ON430_001430"
     CDS             11683..12243
                     /locus_tag="ON430_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR3219354.1"
                     /translation="MNKQELINELKKISLEKQHKALTHPESKNYYEGMVMAYSQVVHM
                     VNTVLKLDEPQKPVVPKFVAEWFEENKDDLEFEIWSLCVNSYCSTTGQEMMNWIQQSE
                     NKPIETLIRMKEGYEVEKEPLYEVIIGDLYLIKKFNNRNDFCFDTSCSLCAWEKSAYQ
                     LTEAEIKAIDERYWPFAVPVEEVAEG"
     gene            12240..12413
                     /locus_tag="ON430_001431"
     CDS             12240..12413
                     /locus_tag="ON430_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219355.1"
                     /translation="MSRPLMLHIPDGTKAIAISIVAEQKDGGLALKTKGIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            12410..12778
                     /locus_tag="ON430_001432"
     CDS             12410..12778
                     /locus_tag="ON430_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219356.1"
                     /translation="MNEEIKKSIRYIDRALSQLEPLRIESHKGMSVYPNQYDKMNADL
                     RNAKLALINIKNNKGCQYCKHSEPLNDTSNSNFATAIECEENGYYIATEYDDGDYADN
                     GYSAYIQYCPMCGRKLEVAE"
     gene            12775..13080
                     /locus_tag="ON430_001433"
     CDS             12775..13080
                     /locus_tag="ON430_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219357.1"
                     /translation="MNVQVYLKRNLARFPRSWDTNSHPSEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            13104..13307
                     /locus_tag="ON430_001434"
     CDS             13104..13307
                     /locus_tag="ON430_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HCR3219358.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            13304..13621
                     /locus_tag="ON430_001435"
     CDS             13304..13621
                     /locus_tag="ON430_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219359.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMQTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVVRLVDMEVINNDCKWE
                     EMR"
     gene            13618..13761
                     /locus_tag="ON430_001436"
     CDS             13618..13761
                     /locus_tag="ON430_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219360.1"
                     /translation="MKELILNKENILDLLDCVNAWILLYGYENMRPEMKELASKLSEA
                     IEV"
     gene            13958..14374
                     /locus_tag="ON430_001437"
     CDS             13958..14374
                     /locus_tag="ON430_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR3219361.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLIQYR"
     gene            15090..15257
                     /locus_tag="ON430_001438"
     CDS             15090..15257
                     /locus_tag="ON430_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219362.1"
                     /translation="MYRPQYLEQKYEVITVQNGNSEIVRKYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            15283..>15564
                     /locus_tag="ON430_001439"
     CDS             15283..>15564
                     /locus_tag="ON430_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3219363.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSSKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTA"
BASE COUNT         5406 a         2387 c         3217 g         4554 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttatata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta atagtttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ctaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gtaaagttcc gtcttgtttt gtatcaaata
      541 taaaatagtg tcttaatttc tttatattta atttccaacg aattttttta ttctctttga
      601 tcaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagagt ttcttccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggttttat atcgattaga gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1261 tgaataaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctattcgctt ttatagtttt gctttttctt tttgccgagc
     1381 catcaggttt gtaccctaat acagctctta gtctatatgt ttcattgtct acttgttcta
     1441 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1501 ataaagaagc ctattgtgta ggtttgtttt ttcttagaac acgctcgctt tggtcggtgg
     1561 ggcgtgtttt ttatactaat tagttatgaa gagcaattga aactgtctct gttccaaagt
     1621 aacctgtttg tgcttgcaat acattatttt gtgcatttgc ttgggcttca gagatatcaa
     1681 aaactatttt accagtttgt tgcatatcag gatttaagct ttccataaag aaagagttag
     1741 tgatattacc attttcatct tgatttgccg acatagatgc agccgaatca gcttcgaaag
     1801 tttttccatc tgcttttaat ttgaagaatg agctatcaac agtcacagcc ttatcacctg
     1861 cattttttac agataagtca actactaaga aagtgtcttt tgcgttagta ggaagtacag
     1921 atgggccaac ttgttttact acttcaacgg agtttacttt gtattccatt ttccctacag
     1981 caacgtcatc accgatttta taagtctttt cttcttttgt tgtagtttct tttgtagttt
     2041 ctttattagt acttgttgat gttgcagtag ctgaatcttt gttttcaccg ccacttagag
     2101 caccaccaat accaaaaaac aaaagtacta ttaataccca aaaccatacc cgtttataaa
     2161 acggtttttt aaccttatac attttcccat cttgacccat aacttttttt gccatttaaa
     2221 tatccctcac ttcttgttat aatatatttg tgatctcaga aatgaggtat gagtccgtgt
     2281 tgcagcacgg gcttttttta ctgtgcataa gagtattttt tcttgaaata tgactggcaa
     2341 acattaaaac attctgttct taacttatta ttgatagagt agaattccat gaaattttct
     2401 aatttgaact gagattcatc agtcaattcg ttctcaataa agatatttag taaaatcata
     2461 attgcgattc tatcagcttc agcttcgaat tttgaatgaa aagttgtaga gttatcgtac
     2521 agtactgaat attcaaaatg tgaagcaatg aaatgaccga gctcatgggc taaatgaaaa
     2581 gcttcagaac tgtcttcgtg tagtttttca ttcaaaaata ctattcttgg ttttggataa
     2641 taaaaacctg gttcttccat ttccatatag attaacttta aattatactc actcagcatt
     2701 tctttcaact ttaaatacat acaacccatc actccaacta ttcattttcc tctaaagctt
     2761 tagcaattgc aatcgcttta cgcattgtct ccttagatat ttcttttcca tcaaaagaaa
     2821 atacagtatc atcttctgat aaatccacat gtttaggcgt ttctttttct tctctaccta
     2881 gaagatagtc tacagagaca ttgaaatagt cagctacctt ttgcaaatct cttgctttgg
     2941 gttctgttgt tttccatcga tagaaaagat tttcgctaaa tcctaattct aaagcgactt
     3001 ctttcatgct tttatctctt tggctagcta atgattttat cctttcaaac agtgtcataa
     3061 taatgttctc caatcctaaa caaataaaag tacacaaaat gtaaattata ctgttgacat
     3121 aagtataagt ttgtattatt atttgtttgt aagttaattt gatagaaaaa aagcaaacta
     3181 aaaatacacc ttatagcatt aagtttggcg accgattgca taataaaggg tttgttgtag
     3241 gcttatttaa ctatgtttat atagtatacg attgtatact attagtcaat agtttatacg
     3301 ttttttctat cattttgact tacaaatcta tatgtaaggg gagtgaatag atgtcaaatc
     3361 tagataacgg ccgtgctgca atcaagaagt ttatgcgaga aaacggagta actatggaag
     3421 atttggctac tgcatatgga tacacacgtg ttcgaatgca gcaaattatt gatggccatt
     3481 ggagtggacc aaaagctaat aaaactatct tagaaatcat ccgtgattac agaattcgat
     3541 aggaggaatt aatcaatgga acaactagct ttagttaacc tctcagccct caaagcttct
     3601 ttggcagaat ctgagattgc aaatgaagtg tgggatacaa agcaagctgc ggaatatttg
     3661 aaaaccacga cacgcacgct aactaaagat gccgaatctg gaaagattcc agcagcgaaa
     3721 gttggcagag agtggaggtt tagtagtatc gctttgtatc agtatctaaa aggaggaaaa
     3781 acatgggcaa attcaacaga gcactagtat tcagcgcacc gctaatcatc tacgctttag
     3841 ggctttgggg aaatagacaa gcgttgatag gaacgattgt gtacatggtt tggattttta
     3901 tagggcttga tgaagctgag tacagagcga aaaaaagaca cgaacgccag caagcatgat
     3961 cgtgtcaagg aaataaacta tctttctata ttttaccaca gaaaggaatg aaccgtaaat
     4021 gctaattgca acggatacac tagaccgcat ctttttaaaa gacgaataca aactgcgcaa
     4081 tatagatgcg tcaggaattt tagtatttga tctttatgac aatggaaaaa ttggtatcta
     4141 tcaagcaagt gatattgaag aaacaaacct tgctttcgag caaattgatg attctgtgga
     4201 attggattta gatgaggcaa tcctagcttt tgaacaaatt gcaaaattat taaaggaggc
     4261 acaaaagaat ggcaactctt taccaactca gcgagtcata tatcaaagtc ctggaactgg
     4321 cagaacaatt ggatgaagaa attcttcgtg atactctcga ttcgattaat gaagcgatcg
     4381 agtataaagc agaaaactta gcaaaaatag ttaaagaagt agaagggaaa gcagagttaa
     4441 tcgatagtga aattaaacgt ctacaggaac gtaagacatc gcttttgaac aatgctaaga
     4501 gtatcaaaca ctatttacaa gaggaaatgg aaaagactgg taaaacgaag attaaaggcg
     4561 aattattcaa cattggaatt caaaataatc cagtatcggt caatgtaatc aatgagaact
     4621 taattccaaa aggattcttt acccctatgc ctcccaaatt ggataaaaag caattgaagg
     4681 aggaactgaa gcatggagat attcctggtg ctgaactcgt ccaaacaaaa ggtttgagga
     4741 taagatgatg caagtggagg tatgagtaat ggtaaaaaaa ataaaagccg aagatctatc
     4801 agttgaaaaa ggaacttaca tgatttacgc aaatcctggt atgggaaaaa cttattcttt
     4861 aggatttctt ccaggtaaat cattggtatt agatgttgac ggatcatcat cgacattggc
     4921 aaagcatcct aacaaagaaa atattgaagt gtgggaatta gattcttctg atatttggca
     4981 agaatggctt gatactattt cagacttagt cgcaaataaa tcttcctacg aaaagcaatt
     5041 cgacaatatt tgtgtagata acatttctga attgttcaaa gcgcaattgg aagatttagg
     5101 caagaaagga aaaaatagtg gtgttccttc tcaagccgac tatcaaagaa cagattttat
     5161 gaatttgcga ggtcttagag cattgaataa tttagattgc cgaatagtcc tgactgcatg
     5221 ggaaacaaca gacacatata cagaacctaa tggacaattt tttacaagat caatgccaga
     5281 tataagatca aaaattttaa ataatttttt aggcttatgt gatgtagttg gcagacttgt
     5341 aataaaaaaa gatgacgatg gtaatgaaac aagaggctta atactaaagc ctactagtaa
     5401 cgtttatgca aaaaacagat tagatgaacg tagtgggtgt ttggtggaac aattggttgt
     5461 tagagcaggc ggtgaaccga atgtatcaac tccgaccgta tcagattaag ctagttcaag
     5521 aagccagaaa acatctatct caagggaaaa agggagtatt gatccaatcg ccaccaggaa
     5581 gtggcaaatc agttgtaatt gcagaaattg ttcgattagc aacaagaaaa ggtggcacag
     5641 tactatttct tgcccacagg agagagttac ttgataacat tcgagaaact cttgaacaaa
     5701 atgaagtaga tttatcaaaa gtcataatat tgtcagctgt aatggctaaa aacaggttaa
     5761 ataaattgcc aaagctgtct ctgataatta cggatgaagg gcatcatggt aaagcgaaaa
     5821 cttatatgga tatttataac cattttaaag aaattcctag actaggtttt actgctacgc
     5881 catacagact caacggagaa ggatttacag atatatacga agaaatggta gaaggaccat
     5941 ccatacagtg gttgattgat catcacaatc tagcgcctta tcgttggtac tctattcctt
     6001 tgattgatcg ttctaaagta gattttaaaa atatgtcacg tgaagctgaa agttctgcgc
     6061 gattattcga gtcagatgcc acaattcaag gtgacattgt taaaaattat aaaaaatatg
     6121 cggatggtca acaagcaatc gtttatgctc caacgattca ggtaagcaag atgattgtta
     6181 aatggtttaa cgacaatgga atatatgcgg ttcatgcaga tgggaaaaca cctaccaaag
     6241 aacgtgatga tattatggca aatttcaaat cgaaaaaaat cactatatta tcaaatgtcg
     6301 atttaatcag cgaaggattt aatgtaccag atgttggagt gattatcctt tgcaggccta
     6361 cgcaatcaat cgtcttacat ttacaacagt ctatgcgagg catgagatat cgagaaaata
     6421 agacttcgat tgtgttagat catgttggga acggagctaa cttaggttta cctactgatg
     6481 aatttgaatg gtcgttaagc gctaggaaaa agaagagtaa tggaagcagc agcgaagcgc
     6541 ctagaatgac ttgctcaaat tgtggacagc agtttcttct gaagagctta ttaaagatag
     6601 aaaataaacc acattgcccg ttctgcttac aagaaatagt aagtgaagaa aaagaaaatt
     6661 ccgttacttt tgatgaagcg gttcaaatgg tggaattgaa tgcagaaaat gccaagctag
     6721 cgcgactttc aagaaagaaa ttttcaaaaa aacaatcttt agaattaaat tatgcgattg
     6781 caaaagcaaa ggtaagtttt gaaggtaaag ggaatccgct ttttaaaatg tttggctcac
     6841 tcactgctta tcaaggacaa cattactcca tcgatcaatt agaagaatta tcgttgatca
     6901 aagatgtatc aatggaatca attttaagag cttataaatg ggctttggaa aaactcaatt
     6961 caaaacaaga agaacccgaa tgggtaaaaa atacatttta ttaaggaaga ggtaattaat
     7021 tatgacagca tttaaagtag attacaatga agcacaagat tttggagcag taccagacgg
     7081 agattatgag gtagttattt tcaacgttac ggaagatgcc tcaaaaggcg gcacagagtt
     7141 tattaatttt gatatggtca tcagaaatga tattaaacaa ccacgacaaa acagtcatct
     7201 tttccataga gtttggaaaa gtaaagaaac tggcaaatat aatcgtggga tgatcatgtc
     7261 gctagcaaaa tcatttggcc ttcctgatgg aaaggaatat caatcattcg aaaatttcct
     7321 tgaagatttt gctatgagac cagcgaaagt aaaagtaaag aacgaacaat cggagtacaa
     7381 cgggaaaacg tatgacaata caaatattaa aaaattcgaa accactaagt ttccagaact
     7441 ccaacatcaa tggaagaaaa acaacgctga aaaatctgta aattcctcac cagcatttga
     7501 tatttcagat aatgatcttc cattctaatg aataattacg agcgtattcc cttagagttg
     7561 cgtgagttaa atcagtgggg gatttataaa cgctcatgga acgaacaacg agggaaatgg
     7621 agtaagaaac cttatgatcc gtttactgga gaattaggga gcagtacaaa tgagagcaag
     7681 tggtctgatt tcaagaccgc tctctcagct gtttcaactt ttaatgcaga tggcctagct
     7741 ttttatttta aaccacctta tataggtatt gatttagatg atataggtga tgatttagaa
     7801 aggtatcttc aaggcgatgt agaaaataac ttagtttacg tttttatgaa ttctacaaaa
     7861 acatattcag aaatatcaat gagtggcaag ggaatccata ttataggtaa agcagcaatc
     7921 cctggagaaa gacgtagaaa agggaatgtc gaaatgtata ccgaaggtag gttctttgct
     7981 attacaggta acttctttgg taataatgaa gaaattaatg agatacctga aatccaaatg
     8041 aatttcttat acaagcgata tttagaaaat gaaacagtaa taaaacaaga tttttctaaa
     8101 agtaattggt cagatggaaa tgacttatct gtcaacgaga tcatacaaac agcggtgaat
     8161 tcttccacag gtaatcgttt tagattattc atggatggag gatgggaaaa aatatatgat
     8221 agccaatctg aagcagattt agcatttgca aatgatttag ctttttggac tgctggagat
     8281 tttcaaaaga tggatgaaat attccgaatg tcttcgttgt ttcgagataa atatgatcag
     8341 aaacgcggaa agacaactta tggaataggg cttttaaata aagccatatc tgagaatacc
     8401 aatcattata ctggcaaaaa aacagctgat gattattttc tttccatccc aggtatcact
     8461 gtggacgaaa ctaaaccgac taagttttat agctatgatg atacaggaaa tgcagaaaga
     8521 tatcttgatt tgtttggatc atttacaaaa tacagttacg taaataaatg ctggtatttt
     8581 tataacggta aaaactggga acaagacaat atcggtgccg ttcgaaaatg ggtagatcag
     8641 actatcgaga tattcaaaaa cgaacctgtt tcgataccta aagatgcgac tgaagatgaa
     8701 gaaaaagcct atattgaagc aaaagagaaa catttaagaa ggtctagaaa caatgctgga
     8761 aaagaagcta tgacacgtga gttgaaacac caagtagcga tacttcctga tgaatttgat
     8821 agtgatgata tgctgttcaa cgctcaaaac ggttatttag atctttctaa tggcatttta
     8881 tacgaacacg atatttctaa aatgttcact cgaatttcta acgctgagta tacggataaa
     8941 agtgattgtc cacgttggaa gctgtttttg gaacagatat ttgacaataa cactgaattg
     9001 attcgctata ttcaaaaagc tgttgggtat tctatgagcg catcgatcag agaacaagtc
     9061 atgttcatac ttttcggtaa tggaagaaat ggtaaatctg ttttcttaga tatcatttct
     9121 gaaataatgg gaagttatgc catgggaatg caggcatcaa cattgatggt taaacaaggt
     9181 ggtagcagcg gccataacga agatatcgca cgattaaacg gcgcacgtct agtaacttcc
     9241 tcggagccaa atgaaggcgt aagaatggat gaaggtttaa tcaaacaact aactggcgga
     9301 gataaagtgt cagcatctta taaaggaggc cacatgtttg actataaacc taaatataag
     9361 atttggcttg caactaatca taagccaatc atcagaggaa atgatgatgg tatttggcga
     9421 agattgccat taattccttt cactgtacaa attccattgg ataaagtgga taagaatttg
     9481 aaagaaaaat tgatgcgcga attgccagga atattcaatt gggcagtaga aggatgtttg
     9541 atgtggcaaa gagaagggtt gaatccaccg gccgatattc agagagctac aatggaatat
     9601 cgaaaagaaa tggatattat aggtgctttt atagatgaat gttgcgaaac aggacctggt
     9661 tattcaatcg gggcaacgga tctatttaaa gcatacgata aatgggcaag agatatgaat
     9721 gaacatccat tcagtcagac ccagtttggt aagaaagctg cggacaggtt tgagaaatca
     9781 aaatcaaaag gaaaagttgt ttatcgaggg attgatttaa aaaaagagtt tagagaattt
     9841 aacgtattag ttcctggatt gtgaaacaaa aaagtttcac aaatgtgtgt aggtagacag
     9901 ttgggtagat agtaagtaga tagtttttta caaactgtct acccgataaa acctttgggg
     9961 ctgtaaggct cattactgtt aggtagatag tagggatagt ttatatatat agtaaataaa
    10021 ataataaaaa taaggaaata tataaaaata cagaagcaac ctagaaataa ctgtctactg
    10081 tctacccgaa ctggttaata cttagagcca caagggatag agcgattata aactgtctac
    10141 ccatgtatat atactatcta cccgagcggg aggcgcttat gacagcagaa attgaaatac
    10201 aaaatgccat tcgaagagaa ttaccaaaat atggtcattt tgtttataga ggcaatgtgg
    10261 gcaaagtgaa gacgattgat ggcagatggt ttgatactgg attgcccaaa ggatggccag
    10321 atttatttgg atggactaaa gagggaaaat ttttcgccat tgaagtgaag aacgaaaaag
    10381 gaaagttgag accagaccaa gtgaagtttg gtgatttttt gcaaaagcaa ccagtcctat
    10441 atggtgttgc acgatcagtg gaagaagcat tgaaaattgt ggaggaatca tcatgacaac
    10501 agaagaagtg attcaaatgc gaattcgaag cattcaacgt gaaattgacg atctagaacg
    10561 aacaaaggca gtgatggtca atgaaacggc gagaaaggca atcgatttgc acatagagaa
    10621 tttaagaagg gaaattcgta gattggagga ataaacgtgg ataagaaagc aacaatgaaa
    10681 cgaattgttg aattaaccaa gtcagaatct tggcaagaag acaaagaaat agttgcagaa
    10741 gtccaaagga tcggcaaatc aatgtggact gaaaagccca aacggagaac gccaagaaaa
    10801 attgcaatct ggcatggtga tcgaattcta gtaacaggta ctgctgaaca gttatctgaa
    10861 attactggat taagcaaaaa catcatctgg gatagagcta ggagcttatg gattgattca
    10921 aaaggacgac agtttaggta tgtggaggag aaataatgga tctcattaca caatatagtg
    10981 atatcatcct caagaaaatc atgatgaaga ttcagaaaga caaaaaatca aaagaacgag
    11041 cggaattagt taagttggaa atggctgaaa caggagcagg agtgcgaagt agcaggcatt
    11101 ggaaagcagc agcaaacatt gaattttatt acaacgaaat tcaaaaaggg ttcgatcaga
    11161 tgcgtgagct ggatcggcaa acaaattgga gcaagaaact tcatcaagat cgtttcaaat
    11221 ttgtagagaa atataaagag atattagacg aatacaagga ggacagcaaa tgataccgaa
    11281 gtttagagcg tgggataaaa aaacagaaac tatacaagag atagaatcaa tatctttcaa
    11341 agaaaaaaag ctagtgattg atcaaaaatc tgtcacttgg ttcaactcgg attatattag
    11401 aaactttggc gaagttgaac tcatgcaatc cacaggactg aaagataaga atggtgtgga
    11461 gatatttgaa tgggatatag ttcttgtcaa tgtaagtaat ggttttgatc atttagttaa
    11521 tgaaaaaacg gttgtacaag aatccgagtt tcattctgga ttgatttata aatcattagc
    11581 tagtgggatg gaatatagag tattcaaaca caaaaagatg gattatgaat acgaagtcat
    11641 cggaaatata tacgagaata gcgagttatt ggaggaacag cgatgaataa acaggaattg
    11701 attaatgaac taaagaaaat ttcactagaa aaacagcaca aagccctgac gcatccagaa
    11761 agcaaaaatt actatgaggg catggtgatg gcttattctc aagtcgttca tatggttaat
    11821 acggtgttaa aactagacga accgcagaaa ccagttgtgc cgaagtttgt ggcggagtgg
    11881 tttgaggaga ataaggatga tttagagttt gaaatttgga gcttgtgtgt aaattcttac
    11941 tgttctacta ctggacaaga gatgatgaat tggattcaac aatctgaaaa caaaccaatt
    12001 gaaaccctta tccgcatgaa agaaggttac gaggtcgaga aagagccgtt gtatgaagtt
    12061 attattggtg acttatatct tatcaagaaa tttaataaca gaaatgattt ctgttttgat
    12121 actagctgct cgctgtgtgc ttgggaaaaa tctgcttatc agctaacaga agcggaaatc
    12181 aaagcaattg atgaaagata ctggccattt gctgtgccag tggaagaggt ggcggaagga
    12241 tgagcagacc tctaatgctc catataccag acggaacaaa ggcaattgca attagcattg
    12301 ttgctgaaca aaaagatggc ggtttggcgt tgaaaacaaa aggtattgat accaaacaga
    12361 ttttagaagg cagagatgtc gagatagaga tcgatgagga ggaagcggaa tgaacgagga
    12421 aataaaaaaa tctattcgtt acattgatag agcgttatcg caactcgaac ccttgcgaat
    12481 tgaaagtcat aaaggaatga gtgtctaccc aaatcagtat gacaaaatga acgctgattt
    12541 acgtaatgca aagctggcct taattaacat taaaaataac aaaggctgcc agtattgcaa
    12601 acacagcgaa ccgttgaatg ataccagcaa ctctaacttt gcaactgcaa ttgagtgtga
    12661 ggaaaatgga tactacattg caactgagta tgacgatggc gattatgcag ataatggcta
    12721 ttctgcttat atccaatatt gccctatgtg cggtcgaaaa ttggaggtag cggaatgaat
    12781 gttcaagttt atttaaaaag aaatttggcc aggtttcctc gaagctggga tacaaattcc
    12841 cacccatccg aggcagaaga agagagctat ctaatgtctg ctaacatgat agaaattaca
    12901 agagatgatg cggatgagtt tgataaaaaa agatcatggt actcggtttc gaatccaatg
    12961 tatacggctg taatagaaga ttatcatata tccgacagat ttattctggt tgatctagaa
    13021 agacctaaga aacgaattaa acagtataga cgttgcgggt tttcaacaaa gaaatggtga
    13081 aaacaaaaca gaggtgactc agaatgaaca acagacatcg cagaataaca aaactaagaa
    13141 aacaggaact gaatgtacta aagacaaagt ttgaaaaaga atacggagtt tctgcagaag
    13201 aaacatataa agtggcaagt cagtgtgttg ctgatgcaag tgatgctatt cgtaagtttg
    13261 gaatttcgat attaagtgat gatcgtaaat gggaggaaaa agaatgaaac taaaagacgg
    13321 attttacgct agtagccacg gtatcggcgg tttaatgctg gatatgcaga caaagaaccc
    13381 taaaacacgt aagaaaccaa aattcaaagt cggtgacatg gttcgctgcg aagcagaagg
    13441 gttcatctat ccatttcgtg gatatgtaga acatgtctat aatcactcag cgatcattcg
    13501 cattgaaaac acgatggaat gcgacaagtt attagcgaaa agcaaagaga atttagcagt
    13561 ggtgagattg gtggatatgg aggttataaa caatgattgt aaatgggagg aaatgagatg
    13621 aaggaattaa ttttaaataa agaaaatatt cttgacttat tagattgcgt gaacgcttgg
    13681 attctgctgt atggctatga aaacatgcgt cctgaaatga aagaattagc ttctaaatta
    13741 tcggaagcga ttgaagttta gatttagcaa aggaggatac agaatgttag taaaaattat
    13801 tgtaggagtt atcttttgga ttatgacttt gatgctaggt tactttatcg gaaaagaaga
    13861 aggtcgtaaa gaaggcaaat aaaaaagccg gatcgctccg actaacataa taaaacagac
    13921 aagtttatta tatcacataa aggagcggtt tgacttgatg caattgttac gagaggtaga
    13981 tttcaaacag acaagatgta atgcgagaga tgtgctgaag aactttcggc gtttggagcg
    14041 tatggcaggt cgctctttga tagatattaa gtcgccgatt ataacggata tgccgaaggc
    14101 gccgaagcac ggcaataagg cagaggacgc gatcattcag atgatggata tagaagcaga
    14161 gagagatgcg attctagcgg ctttgatggc tcttagtctg attagtcgtc agatactcta
    14221 ctacagcttc tgcgacgtaa ataagcattc taattatgaa ataggtcaat tgatacgagg
    14281 atacggagag aagaatgtag agaagttgaa atccatcgca ttgattgaat ttgctgaagc
    14341 atataaaaaa ggcgtattaa ttcagtatcg ttgattttgt agggtttttg tagggatagt
    14401 gtagggtttt tgagcggttt aacgtgatat tatggtagtg tcgaaagatt aggaaacagg
    14461 acttcgacaa aataaaatgt aagggaggaa atctccctca tcgtttaatt aagcttcgat
    14521 agacagcagc ggaaatatta agaataagga tgtgaatttt aactccttct aaattgttct
    14581 tattatctat catccgttgc tgtctattgt cattatgtca ctgtggcgga aagggtagac
    14641 gcttaaaaat aaggtcaata cgtcgaggga tagccttaac gttttatgat ttgaccatgc
    14701 aatgttcgat tcattgccag tgacttaagg aacctacgga aacaattcat cttatcggat
    14761 gccgatgaat tggctgacta gtcgggatgc tactagcagt tagaaggcat aaaatactag
    14821 cgcagacgtg cgccactctc aggtgtaggt taggagagaa acattagttg gggttattag
    14881 gaatacgata acctgcttgc gacaaagctt tgtactgtcg cgttggtcat gaacagagac
    14941 ggtattctgt ttcagtattc gttagcaacc gagggatgtg gcagtggtga ggtgcaggaa
    15001 gtatcagact tgtttgtgtg caagttacta ttacatatta gatcactctt tgagtggtct
    15061 ttttattttg cacaaaggag gtaacaacaa tgtatagacc acaatactta gaacagaagt
    15121 atgaagtaat cactgttcaa aatggtaaca gtgagatagt acgaaagtat agaagaccaa
    15181 taaagagcga tacatataaa cgaaaggaaa acaatgaagt tattccattg tatggcaaaa
    15241 gaatagctaa gcattaaata agattgcgaa aggagacggg acatgactga ggaattctat
    15301 agatggctat tacagttgat aagagaagat cgtttggtta agttctatca gtcttctaaa
    15361 tggcgcaggc ttagagagaa agcgatgaaa cgagatcact atgaatgcca agagtgtaga
    15421 agactaggta agtatcatag agtagagaac gttcatcata taaaggaagt caaggataga
    15481 cctgacttag ctttagattt agataatctt atttgtttat gtgttgaaca tcataatgaa
    15541 gttcatggca gatatcttac agca
//
LOCUS       DAJSMI010000059        15481 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJSMI010000059 DAJSMI010000000
VERSION     DAJSMI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.059"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(270..1766)
                     /gene="lysS"
                     /locus_tag="ON430_001440"
     CDS             complement(270..1766)
                     /gene="lysS"
                     /locus_tag="ON430_001440"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR3219364.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="ON430_001441"
     CDS             complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="ON430_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR3219365.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="ON430_001442"
     CDS             complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="ON430_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR3219366.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="ON430_001443"
     CDS             complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="ON430_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR3219367.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="ON430_001444"
     CDS             complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="ON430_001444"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR3219368.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="ON430_001445"
     CDS             complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="ON430_001445"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR3219369.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8297..8767)
                     /locus_tag="ON430_001446"
     CDS             complement(8297..8767)
                     /locus_tag="ON430_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCR3219370.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8813..9238)
                     /locus_tag="ON430_001447"
     CDS             complement(8813..9238)
                     /locus_tag="ON430_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCR3219371.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9305..9574)
                     /locus_tag="ON430_001448"
     CDS             complement(9305..9574)
                     /locus_tag="ON430_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCR3219372.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9585..11180)
                     /locus_tag="ON430_001449"
     CDS             complement(9585..11180)
                     /locus_tag="ON430_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3219373.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="ON430_001450"
     CDS             complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="ON430_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR3219374.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14733..15293)
                     /locus_tag="ON430_001451"
     CDS             complement(14733..15293)
                     /locus_tag="ON430_001451"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR3219375.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         4013 a         3477 c         2729 g         5262 t
ORIGIN      
        1 ttttgttatg tttttgaata aatattttct tctgtctttt ggacaagtaa taatatacca
       61 actaaattta gcttggtcaa cgaatttttt gtattttttc aagttttttt caataaacga
      121 ttattcataa cttttctttc tttatcgttc gttttcgtct tctttaatgt ctagaatagt
      181 cggcattagc ttttgaactt tcgttattat ttattccttt ctacacagca aaagctgcct
      241 aaggaattcc ttagacagct tttattcgat tatctcattg ttgggaatag caaaacatcc
      301 cgaattgatt gtgcatcagt tagcaacatt actaatctgt cgatcccgat gcctagacca
      361 ccagttggcg gcataccgta ttctaaggct tccaagaagt cttcgtctac accgtgagct
      421 tcgtcatttc ctagttcacg ttctttttct tgcgcttcga atctttctct ttgatcaatt
      481 ggatcgttca gttctgtaaa ggcattagca aattcgcgcc ccactatgaa tagttcgaat
      541 cgatctgtga atcgaggatc ttctggattt ttcttcgcta aaggcgaaac ttcgactgga
      601 tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca
      661 ttgataacat gtccgacacc catagcttcg gttacttcta cattgtgttt ttcggctaaa
      721 gctaatgctt cttccaatgt catttcttgc cagaagtcta caccagtttg ttctttgatt
      781 gcatctacca tatggatgcg tttaaagtca ctttctaagt cgacagcttg tccatcatat
      841 gtgatttttg ctgttcctag tactttttca gctgcgttac ggataatacc ttctgtcaga
      901 ttcattacat cattgaaatc agtatatgct gtgtatgctt ctaacatcgt aaattctgga
      961 ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc cgatttcata aactttttcc
     1021 attccgccaa caattagtcg ttttaaatga agttctagcg caatacgcaa atataaatca
     1081 atgtccagtg cattatgatg cgtgataaat gggcgtgcgg cagctccacc tgcctcattg
     1141 tgcaatactg gtgtttctac ttcaatatag ccattgccat ctaaatagcg acggatttcg
     1201 ctgataattt ggcttcgttt cataaatcgg tcaaagcttt cgcgattact gattaaatcc
     1261 aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc catggtattt atctggcagt
     1321 ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg
     1381 tctgttttca tgatttgtcc agttacacca aagaaatctc caaggtctgc atgtttgaat
     1441 acttcatagg cctcatcacc tacttggtct tttcttacat aaatttgaat ttggccttca
     1501 cgatcttgta gatgcgcaaa tccagctttc ccttttcctc ggcgagtcat catacgacca
     1561 gctacactag cggtcaatcc catttctgct agttcttctt ttgttcttgg atcaaatagt
     1621 tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca
     1681 tcttcacgca attgttccat tttttgacga cgtacaagca tttgatcatt taaatcttct
     1741 acttgatgtt gttgttcctg tgtcacaaaa gttcctccat tctcatgtta atctctttcc
     1801 tataatgcca atgaaacaca aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc
     1861 acgaaaaact tagaaatcac aacgtttgtt tcaatagtac tttttccgct ttttctacaa
     1921 attgatccag caggtcattg atttcttgct gtttttctgt ctgattgatt gctactttca
     1981 ctttcgctgc tcgaggaatc ccttttaaat aataagccgc atgtgttctg aattctctac
     2041 aagctacttt ttctcctttg agatctgcta agcgttgtaa atgaagttta gctgtagcaa
     2101 ttttttcggc tggtgttggt tcaggaatca gttctcctgt ttcgagataa tgctgtgtgc
     2161 ggtgaatcat ccatggattg cccagcgctg cacgaccaat catcacaccg tctgcaccga
     2221 catagtcaag catccatttt gcatcttctg gcgttttgac atccccgttc cccataaatg
     2281 ggatcgtcaa atgttttttc acttctttca atacttccca gtccgctttt ccttcataca
     2341 tctgaacccg tgtacgtcca tgcatagcga tggcacttgc tccagctgat tgtgctgcta
     2401 atgcattttc tacagcaaag acgtgctctt catcccaacc aatacgcatc ttgattgtta
     2461 ctgggatatt tactgctgaa gaaacagctt ggaccatttc gtatactttc tcaggatcta
     2521 aaagccattt tgctcctgct tctgctttga ttactttatt tactggacag cccatattga
     2581 tatctattat atcggcagct gtgttttctt cgacaaattg agcagcttca actagactat
     2641 ctttatttcc gccaaatatc tgtaaactta atggatgttc tgtttcttca atgtggagca
     2701 tctctaaagt ttttttgttt ctgaaatgaa taccttggtc actgatcatt tcacatacga
     2761 ctaagcccgc accaaattct ttgacagtga ctcgaaatgc tgaatttgtt attccagcca
     2821 tcggtgcaac aacgacacga ttagggattt ccacattgcc aattttccat gtgtgttcca
     2881 cttaaatccc tcctaaggct tctttcttca atccatctaa ttcttcttcg ctatacttgt
     2941 atttgttatt acagaaagaa caaaccgctt ctgcaccgtg atcttcgtct atcattgctt
     3001 ggatttcttc tgcacctaac gcgataattg cttctgcaaa tttttctttt gaacagtcac
     3061 atttgaattg aacaggcatt ttttccaaaa tgttcaattc ttctgtacca agcaatcctt
     3121 tcaatatttc ttctggtgtc tgtccttcat ctaagagggt agagattctt ggtgtcgttt
     3181 ttaattgttc ttcgatcttg ctgattgttt cttcatcggc acctggcatg atttggatca
     3241 tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac caatacactc agtccaacag
     3301 ctgaagggat ttgttctgag atagcaagat agtaagtaaa atcttcagcg atttctccag
     3361 aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa
     3421 acattccttg tgttccaact gctccccgca catcgatttt accttcacta tttagaggaa
     3481 gactgagatg cggatttttg atgtaacctt tcacatctcc ctttccattt gcatctacta
     3541 caattcctgt tgctggacca tcgccttgga ttcgaacagt tagtttgtct tctcctttga
     3601 gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc caaagcagct gaggaggtgt
     3661 gccaagtatc atgacgtctt tgtgcctccg ctacggtatt tgttgcttga acggcgtatg
     3721 ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata atctttcatt tgttatgtac
     3781 tcctttttcc gtttttaaac tcatagtgaa tcatactagc aactactgct tttttcaact
     3841 ttggaatgct tcttttaaaa ataattcaga atcatctaga aaacaggaca tcaccctatt
     3901 tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat
     3961 atcagatcaa ttgacctata tttttttatt tgtattgatc gtctgaatga ttatcttctg
     4021 attgttgaga atctgaatca tgttctgctt tattttcctc attcagttca tgttttgctt
     4081 cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt
     4141 ttgcttcttc aaaagttttc gcttcttttt cgcttggata atcatttgac tcagtacctt
     4201 caggcatcac accgtgttca aacaatgact tgattgcttt ggcatccaat gtttcgaatt
     4261 ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg agcttcgatg atttctcgag
     4321 ctttatcatg agcttccatc aagatttttc gtacttcttg atcaatctcg aaagcgactt
     4381 gttccgagta agcttttgtt tgaccatagt cgcggccaac aaacacttga tggtttcctt
     4441 catattgaac aggacctaaa cgatcactca ttccatattc tgttaccatg cttcgtgcta
     4501 gagctgtcgc ttgctcaaag tcattggaag caccagttga ttggacattg aagatgattt
     4561 cttcagctgt acgtccacca agtaagccta cgatttgttc aaacatatct tctttcgtca
     4621 tcaagaactg atcttctttt ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga
     4681 tgatcgttac tttgtggacg acccgagcgc gactcaatac taatccgaca atcgtatgac
     4741 cagcttcgtg ataagcaacc atttcgcgtt cttttttgct gattacgcga tcttttttag
     4801 ctggacctgc aatcacgcga tcttccgctt catcgatatc agaagcatcg attttctttt
     4861 tatttcttct agcagctact aacgctgctt cattcaagac attttctaaa tctgctccgg
     4921 caaaacctgg tgtctgttga gccacaactt tcaagtcaac gtcatctgcc atcggtttat
     4981 tccgtgcatg gactcgcaag attgcttcac gaccttttac atctggacgt ccaactaaaa
     5041 tctggcggtc aaaacggcct ggacgtagta acgctgggtc tagtacatca gaacggttgg
     5101 ttgcagcaat gacgattact ccttcatttc cgtcgaagcc atccatttct acaagcaatt
     5161 ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat accggcacca cgttgacgac
     5221 ctaccgcatc aatttcatca atgaaaatga tagctggtgc gtttttcttc gctgtttcaa
     5281 acaaatcacg aacacgactt gctccaacac cgacaaacat ttctacgaaa tcagaacccg
     5341 agatcgagta aaacggtact ccagcttctc cggcaacagc ttttgctaat aaggttttac
     5401 ctgttcctgg aggtccttct aaaagtacac ctgccgggat acgggcacct aattcaacaa
     5461 atcggcgagg atctttcaga aattcaacaa cttcaactag ttcttgtttt tcttcttccg
     5521 cacccgctac atcagaaaaa cgtacgcgat tagctttttt gtctgcttct ttggcttttg
     5581 atttaccaaa gttcatgaca cggccgccac cgccaccgcc gccgccttgt tgtcccatca
     5641 tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc
     5701 atataccgct gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt
     5761 ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag
     5821 tcgtatctgt tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt
     5881 tgtattcacc agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct
     5941 tgcctgcttg tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt
     6001 tattaccaaa aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca
     6061 aggcattttt catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat
     6121 ttaatttaac agttttttat caatcctgac cattctattg taccataatt taattgagat
     6181 acagcattaa tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg
     6241 atatgcttct gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc
     6301 aacataatct gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat
     6361 tttgactgat tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc
     6421 gctgtcaatg atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc
     6481 tttgacgatt tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat
     6541 gaaatccaat tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc
     6601 tccttttaag acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt
     6661 gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc
     6721 cttttccaac atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg
     6781 tagtgtattt tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag
     6841 ctatatatat ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt
     6901 actttctgat cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg
     6961 cggtctcctg cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca
     7021 tgagaaaata gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc
     7081 agaccaatcc attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc
     7141 tggtcttctg tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac
     7201 tctcttttca gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc
     7261 cactggtcta aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat
     7321 gctttgtcga aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc
     7381 atttctgcag aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt
     7441 cccatatatt tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa
     7501 tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga
     7561 tttctctgga tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc
     7621 gctgcataag tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc
     7681 gtagcaaatg gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg
     7741 attttcatta acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca
     7801 tattgtcctt cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt
     7861 tctatggcgg atttggctgg atcttcccaa atacttacat ataaagggag attcttctct
     7921 tcacaatatg agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg
     7981 atgtggacga tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc
     8041 aaaaccatcg aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag
     8101 aatccttgtt cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg
     8161 cctttttcaa cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa
     8221 cgtctaaaac gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat
     8281 cattatttgc ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt
     8341 ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat
     8401 cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac
     8461 gatttcggtt gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag
     8521 attcagcagg ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta
     8581 ctgaaataac tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta
     8641 catagccatt tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa
     8701 taaacgcacc aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga
     8761 ttgacataaa aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg
     8821 cagttgtaga actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta
     8881 atataggata aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag
     8941 ctacatactc atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt
     9001 tgacttcttt catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt
     9061 gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa
     9121 ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat
     9181 actttttctt tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt
     9241 ggattcgctc caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc
     9301 taatctattt ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg
     9361 catcttcttt tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc
     9421 caaactggac tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac
     9481 cgttgatttg aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga
     9541 ttcgggaaat ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt
     9601 ttatttaatc gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac
     9661 agacgaagac gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg
     9721 ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca
     9781 taatacagca gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa
     9841 tgccgttctt tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc
     9901 gtcacaccta atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc
     9961 agaaaagaag tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg
    10021 cgaaacgagt tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca
    10081 atggaaaaaa caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa
    10141 ttgatataag ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc
    10201 aatctaagat agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga
    10261 cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt
    10321 actaaaggct gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt
    10381 ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc
    10441 aatccactat aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt
    10501 gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt
    10561 tcatagtagt gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct
    10621 gccgtccctg tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca
    10681 atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag
    10741 aatcttcctt ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag
    10801 gaaactactt ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg
    10861 ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga
    10921 ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg
    10981 aactgcggaa gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc
    11041 tgataaacat aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca
    11101 cttaatactt ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc
    11161 cgcatttctt tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac
    11221 gcaacgcttt gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg
    11281 ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg
    11341 tcgctgtcag tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta
    11401 gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat
    11461 ccattttgat ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac
    11521 ggtcgagcag atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc
    11581 gtttatagat ctcgattttc tgccgttgat ctgccacgta tgtttcaggt agatacgcat
    11641 cgacaccgag gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac
    11701 gagaaacagc ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc
    11761 catgctgctg agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa
    11821 tcttgaatcc agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga
    11881 cttcgttaag gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc
    11941 ttcctacccg ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa
    12001 cgaacaacgt atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa
    12061 gaacatcata ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca
    12121 tttgcccatg agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt
    12181 tttgttcgat cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt
    12241 ctcgatggat tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg
    12301 gataacgatt cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca
    12361 tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta
    12421 actgtttcaa tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta
    12481 gacccaaatc gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat
    12541 caacttgacc tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac
    12601 gacttaatag cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt
    12661 gctgctgtgc caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg
    12721 ctttaaaggc cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta
    12781 gtcgatccat tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact
    12841 ggtcatcagt ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag
    12901 gtccaaaagc atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag
    12961 cgatatcctc gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta
    13021 gcttattgat gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt
    13081 gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt
    13141 gaacgccatc tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat
    13201 ggacaacgta atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta
    13261 ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag
    13321 ctactatttt ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta
    13381 cctgtcctct gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa
    13441 gcatttgttc tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt
    13501 tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt
    13561 gcatcgtccg atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt
    13621 ggaagagtga gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg
    13681 ctgaaaatgt ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg
    13741 ccgcttccct ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta
    13801 gtaaactatt tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg
    13861 agtagaattt tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga
    13921 agtaatcttg caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta
    13981 gcattttttg catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac
    14041 tcgttgggga aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa
    14101 aataccgcat agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg
    14161 ttaacgggta aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca
    14221 tctcttctcg tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct
    14281 catcaccaat atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt
    14341 tgtgcaaaga tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa
    14401 gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca
    14461 cttcatccac tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg
    14521 ctaactgatt cgcataatac aaatttggca tcagaataat catgttttta tttgttttct
    14581 taaacgctcg agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc
    14641 taggcatttt ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta
    14701 attgaataat atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg
    14761 aacgaatgcc cttctatgag atattcgact gctttaacag attgacacat gctttcttct
    14821 atcaaaggct gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc
    14881 ccttctggcc tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa
    14941 ataatacttt tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt
    15001 cccactgcca gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa
    15061 taagtcatta gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact
    15121 aaaaggatct tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt
    15181 ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt
    15241 tttgtatatt ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt
    15301 ttccacctaa ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca
    15361 tcgagctttt ttctttttta tacttgtcaa tgcatagtat aacacaaaac aaggcgtttc
    15421 ggctaaaaga gaatcgttaa taaatgataa ataaattaaa aatttcttca aagtttaatg
    15481 a
//
LOCUS       DAJSMI010000060        15279 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJSMI010000060 DAJSMI010000000
VERSION     DAJSMI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.060"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(84..626)
                     /locus_tag="ON430_001452"
     CDS             complement(84..626)
                     /locus_tag="ON430_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCR3219376.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            761..1150
                     /locus_tag="ON430_001453"
     CDS             761..1150
                     /locus_tag="ON430_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219377.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1154..1918
                     /locus_tag="ON430_001454"
     CDS             1154..1918
                     /locus_tag="ON430_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3219378.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2047..2994
                     /locus_tag="ON430_001455"
     CDS             2047..2994
                     /locus_tag="ON430_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3219379.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3124..3864
                     /locus_tag="ON430_001456"
     CDS             3124..3864
                     /locus_tag="ON430_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219380.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4155..4823)
                     /locus_tag="ON430_001457"
     CDS             complement(4155..4823)
                     /locus_tag="ON430_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3219381.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4957..5244
                     /locus_tag="ON430_001458"
     CDS             4957..5244
                     /locus_tag="ON430_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219382.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5660..7198
                     /locus_tag="ON430_001459"
     CDS             5660..7198
                     /locus_tag="ON430_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCR3219383.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7195..8160
                     /locus_tag="ON430_001460"
     CDS             7195..8160
                     /locus_tag="ON430_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3219384.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8147..9469
                     /locus_tag="ON430_001461"
     CDS             8147..9469
                     /locus_tag="ON430_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3219385.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9532..9636
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9710..10453
                     /locus_tag="ON430_001462"
     CDS             9710..10453
                     /locus_tag="ON430_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3219386.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10468..11460
                     /locus_tag="ON430_001463"
     CDS             10468..11460
                     /locus_tag="ON430_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3219387.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11472..12209
                     /locus_tag="ON430_001464"
     CDS             11472..12209
                     /locus_tag="ON430_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3219388.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12199..12921
                     /locus_tag="ON430_001465"
     CDS             12199..12921
                     /locus_tag="ON430_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR3219389.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13005..13643)
                     /locus_tag="ON430_001466"
     CDS             complement(13005..13643)
                     /locus_tag="ON430_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3219390.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            13978..14412
                     /locus_tag="ON430_001467"
     CDS             13978..14412
                     /locus_tag="ON430_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3219391.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            14435..14755
                     /locus_tag="ON430_001468"
     CDS             14435..14755
                     /locus_tag="ON430_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219392.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            14896..15087
                     /locus_tag="ON430_001469"
     CDS             14896..15087
                     /locus_tag="ON430_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219393.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(15158..>15279)
                     /locus_tag="ON430_001470"
     CDS             complement(15158..>15279)
                     /locus_tag="ON430_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCR3219394.1"
                     /translation="IFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
BASE COUNT         5128 a         2572 c         3173 g         4406 t
ORIGIN      
        1 tgttttttaa tcaaaataat aaataaaaac tgtctaacat ttaaaattta atttagatat
       61 tagacagttt ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt tttagtccag
      121 aaaccacctg aaatatattt tggctcataa gaaatgataa aagcacgctc ttcgatttct
      181 ttgatcaggc gatacagttc acgttcgctt tttctaggag aaagtatttc gagaactagc
      241 cgctcacctt ccagccccat tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt
      301 atacccggca aacttttatc ttctgcgcta tttgctggca aaatcgttgt gaccatgatg
      361 tatcctaatg ccaatttgtc ttcaatcaaa attccgacac tgatacctac tgcgtaacct
      421 aacgcataga caaataaatt cagtggatta tctaaacgat ctaaaacaag ggataagccg
      481 acgatataaa tcgtgatctc gaccatactg acaagtggag caatcagtcg atagcctttc
      541 attgtcaaca tgaaacgtaa cgtgttcaga gtgatataag caaaattaat gaggaagatc
      601 atccccaaca tttttggatc aatcatttgt aaaactcctt tcatttatat aacaacaata
      661 tcaaaaaaaa tcgtaagtga agaaaaattg aaactttata catacaaaaa aatgataaaa
      721 tgattataat agaattataa agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt
      781 gttttgtgcc agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat
      841 tcaacagcca attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat
      901 tttaagcgtt tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag
      961 ttttttgaat ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg
     1021 ttctctgtgc ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa
     1081 cctgttccag acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat
     1141 aggtgagtaa caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt
     1201 agaagttgtt ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg
     1261 atggcagaca agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat
     1321 tcgtgtgatc ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat
     1381 tccatcctat acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat
     1441 caagcattta gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat
     1501 ggggggaatg acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg
     1561 tttgatgggt tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga
     1621 atataattat gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga
     1681 tttgtcattg cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc
     1741 tgacgaaaaa atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc
     1801 ttacggaaaa gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaatacc
     1861 actaatggaa gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt
     1921 gaaacggaaa atcactcatg acgatagagg attttccgtt ttttattttg ggctataata
     1981 aactctgtta ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa
     2041 gagagcatgc ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc
     2101 tctcgagtca tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca
     2161 gcaatggctg aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg
     2221 tcgcagatca tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg
     2281 aatttgctga tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt
     2341 acaaatgatg ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa
     2401 aaagacttcg attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct
     2461 gaaggatatg attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat
     2521 gccttagaaa gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt
     2581 gaaatttcaa gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa
     2641 atcttgcggt ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca
     2701 gattttacag ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta
     2761 agagggatta taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat
     2821 ggggtatttt tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata
     2881 cgtatgggtg aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt
     2941 caaggttatc gtcgtttttt tcctgaacta gttatcagag agacaacgaa ttaagtctta
     3001 ttactatata tatatttatg aaattcatcg ctattttctt aaaataatca gcgcattgct
     3061 actgaaaaca atgaatctgt gctattattg agaaaagttc catttttgag aaggatggtt
     3121 tgaatgaaag aaatcagacg aaaacatttc cgttttcctg cgtacgatga tgagttaggc
     3181 gtaaaattaa ccaacgacaa gaagcgagta ttatttgacg gacaagaaga tcttttgact
     3241 gaacagaatc agtccccgtt tgaacagatg aaagaatttc atttcacgaa cacgaacacc
     3301 gagaaaatcc gtcgcaagcg tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa
     3361 aatttggaaa agcataaagg aaaattaccg gattataccg gccattacca atcagaaatc
     3421 acatcgacag gacgaaaaaa actttttggt gattcctctc gttctgtcag acaaagtgaa
     3481 acaaggacag agtccaagca tactacacga aacgcatctg ttgacacgaa ggtgcgggag
     3541 cgttcttatt ttgttcctaa atatgtaccc gcatcgatta tcccagaccc tagagaaagc
     3601 aaaatttctg aggatcattt aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg
     3661 ttcgatacag aaccgacgcc gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca
     3721 ttcaatcgaa caaaaacgtt agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa
     3781 agccatggta tcttagaacg ctcattacaa ggattgatcg atgatcaagg aaactctttg
     3841 ggagaaaata gctattttaa ataagtgata tacaaattta tattcggcta cagttggaaa
     3901 ggaaacgctg cggcgtttcc ttattttata ctctatccga aagtttatat cgtatcctca
     3961 aatttttttg agtttaagcg aattttttct cccaataagt aagaaaataa tggcaaaata
     4021 aaaaaacaaa ctattttcgc agatcgtttg caaaaatagt ttattttttt ataaagaaga
     4081 ttttactgaa taatatcatt ttagtttaca taatgatatt tctgtaaatt aaataaagta
     4141 gtgaaaaata agaatcagtg ctctaactgt tcaagaggat caagtggttc aaatgtttct
     4201 gtagtctgtt ttgcttccaa ttggggcaaa acagatccaa tttccagata cttcacttta
     4261 ttcaatgtat aagatagaat agaacagcta agtaatgtat acagaaaatg tgtgagatcc
     4321 aaataaagca aggagacaac taaaatacca aaagtaatca agaaaagact tttgccaatc
     4381 ttaatatgaa aatatttttc aatggcttga taaatgatat ctgttccgcc tgtgcttccg
     4441 ccgaatcgaa agacgagacc gcaaccaatg ccagaaagaa tacctgtcat caaactattg
     4501 atcagtagac tatctacagc aaaatgtata tgaaggctct cccataaatg taaaaagaaa
     4561 actagcgcaa atgttccgac gatcgtccgg agaaaagcgt gttttccaaa aagaaacaat
     4621 gagataagca ataaaggtaa attaaaaagc agtgaagtga cagaaggagc gaatccaaaa
     4681 agattatgca aaagcaaagc aataccagta accccaccat ctgccagctt agatggaata
     4741 ttgattcctg tcacagctaa tccgtagatt cctgcgccta aaatgattcc gcacaattct
     4801 gtaaataaat ttaatttttt catttctagc ctcttttctt ttgaaaattt tcagcaactt
     4861 ctttattata ctgacttata ctcttacttc aagaggaaaa tgaaaaaaaa ttatttttgg
     4921 ctgtttcttt ttatttatca aataaggagt ggaaaaatgc gagcagcaga cttattgaca
     4981 attcttacaa aatcacccaa aaaaacagag tatcaaggag tttttttggc tcaagaacaa
     5041 aaactggtgc ctgtgacaat ggtccagata aataaagcaa atcaatttat ctttttattt
     5101 gaacacggca aagcaccact tgctatgaaa gacatctttc tttttttgat gaagaaccgt
     5161 gagaaagctg tttattatca gtcaaaagaa gaactcattc ctctttatgg agtgaaagag
     5221 atagaaaata aagtggtgat ataagtaaaa ataagaaaac aagacgttta tgatgtcaaa
     5281 acaaccattt tttacatttt tggcaaaaaa gtgtcatgat acagtataat attcgttgaa
     5341 caggccaata aaggtgttct cccccacgag atttctactt tattggctgt ttctttttat
     5401 taaaataaaa gttgtgtgtg taaaaaagat atagctgatt ccaagtgaca atttcgtata
     5461 gatttttatg gtattcgtag tgaaaaaacg agaaccaaaa agttgatccg actttgttgc
     5521 ttggattctt tttttgtcag catatttaga ggtatttttt tctttttttt gttcgtgctg
     5581 taaggttaag gcaagacaag ttagttggga aaaaggaaag aaatgtttat acttaaagaa
     5641 taacagaaag gaaggtcgca tgaataattt tatacaaaca ttaagtgaaa gaaaaggcga
     5701 gttgctggaa gctgcatttc aacatctgtc catttctctc atttctttgc tgattgcagc
     5761 actgattgct attcctttag cgatttgggc agccaatcat aaaaaaatgg cagaagtctt
     5821 attgcagatc acgggtgtgc tccaaacgat tccttcactt gccttattag gtttacttat
     5881 cccatttgta gggatcggaa ctgttccagc attgattgca cttgttattt acgctttgct
     5941 tccgattttc caaaacacgt atattggtct agctgaaatt gacccgtcta ttgaagaagc
     6001 agcggtagca tttggtatgt ccagaatgag acgcttgctg aaagtagagc ttcccatcgc
     6061 attacctgtc attatctcag gtatccgaac tgccttagtt ttaatcatcg gtacggctac
     6121 tttggcagca ttgatcggag caggtggatt agggacattt attttgttag gtattgaccg
     6181 aaatacacct gttttgacat tgattggtgc gatcagttct gccttgcttg caattatatt
     6241 cagcggactg attcgctggt tgcagcataa gaaacctcga tattccatca ttactttagc
     6301 agcagtggtg tgtttatttg gcggcgtttc tttatatcag tctgccttgt ttaaaaatga
     6361 aaccatcacg attgctggaa aattaggagc ggaacctgaa atcctgatcc aaatgtataa
     6421 agaattgatt gaagacaaca caaaaacaga agttgaatta aaaccgaatt ttggcaagac
     6481 gagttttttg ttcaatgcac tggatcaagg tcaaatcgat atctatcctg agttcacagg
     6541 tactgtccta gaaagcttgg tgaaggtacc tgaagcaatg aaaagtgctg atttgacaaa
     6601 agaaaaaaca tatgacgaag cagaagaatt attgaataag caattcgata tgacattatt
     6661 aaaaccaatg gcttaccaaa atacgtatgc cttggcagtc aagagatctt ttgcgaacga
     6721 acatcaactt aaaaagattt ccgatttggc agctatctct gatcagatca aagcgggatt
     6781 cacactggaa tttattgatc gttcagatgg atataaagga atccaagaag catatggctt
     6841 gaatttttca agtgtacaaa gcatggagcc aagtttgcgt taccaagcaa tcaataatgg
     6901 agatgtcaat attgtggatg cctattccac agatagccag ctcaaggagt acgatctagt
     6961 gactctagaa gatgatcgag ggttattccc agcttatcaa ggcgcaccgc tgatgaaaaa
     7021 agattttgct aaagaacatc ctgaaatcgt taaagcactg aataaattaa gcggaaaaat
     7081 aacagaggat caaatgattg atatgaatta ccaagtcaat gtcgaaaaga aaaagccgac
     7141 agaagtagca cgaatcttcc tgaaaaatga aggattagta aaggaggaag atcagtgact
     7201 aaattaatcg agttcaaaca tgtgcaaaaa aagtatgatg gaaagtatgt catcgatgac
     7261 ctaaatctgg caatcaaaaa aggagaaatc tttgttttgg ttggtccttc cggtagtgga
     7321 aaaacaacta ctttgaaaat gatcaatgga ttatctaaac catcagcagg agacatctac
     7381 ttcaaaggaa aatcgttaaa tgaatataat ctgcagaaaa tgcggtggaa tatgggatat
     7441 gtgctgcagc aaatcgcttt gttcccgacc atgacagtca agcaaaatat tgaagtcatc
     7501 cctgaaatgt taggttggga aaagcagaaa agagcagacc gtgtagatga acttcttcaa
     7561 aaagttggct tatctcctga tatttatcga gatcgtatgc ctagagagtt gtctggagga
     7621 gaacagcaac ggatcgggat catccgagct attgctgcaa gcccagatgt gattttgatg
     7681 gacgagccat ttagcgcatt agatccgatt tcaagaaact cattacaaga attggttttg
     7741 tccttacacg aagaattggg cacgaccatc gttttcgtta cgcacaatat ggaagaagca
     7801 atcaagctag gtgatcgtat tgcatttatg aaggatggag aaattatcca atgtgacaca
     7861 ccagagcagt tattgatgaa tcctaaaaac gactacgttc ggcatttctt tgatgaaccg
     7921 aaacagacga aagagtggcg agtagaagat ttagtcgtca atggatattt cttaaatgaa
     7981 ataccagaaa atgctcgtca acaagtttgc tttgatacgc ctatgaaaga agtatattcg
     8041 cttctttcgg tgtatccaag cattgtgata gtagaaaagc aaagaagtat tgggtcgctg
     8101 acaagtaaag aaatctttgc ttttttaagt cgagaggagg aaggaaatga aaacatatga
     8161 tgcaatcatc atcggttcag gtgtgagcgg cttaagtgct gcttatggat tgaaagaagc
     8221 aggtaaaaca gtactagtgg tcgaagaaga tctatggggc gggacttgtc ctaaccgtgg
     8281 atgtgatcct aaaaaagtct tgttaagtgc agtagaggca cgtaatcggg taaaacaatt
     8341 gagtggaaaa ggttttaacg agatcccaac tgccaactgg gaagaacttc aaaaattcaa
     8401 acgaacattt acggatccag tcccagaaag ccgaaaaaaa caattagcag aagctgaaat
     8461 cgatcatctt tcaggaaccg ctcgtttttt ggatgattca tctattgaag tcaacgaaga
     8521 agtcttccat gcggattact tagttttggc aaccgggcaa agacctacga tcttaccagt
     8581 ggagggaaag gaatacttga aaactagtgc agacttttta tcattgcctg tactgcctaa
     8641 agaaatcatt tttatcggtg gtggttatat tgcttttgag ctagctacga ttgccaatgc
     8701 tgcagggagc aaagtaacca ttgtccatca taatcaacgg ccattaaaag aatttgaggc
     8761 gtctttggta gaggaagccg tccatcaaat ggaagcatct ggcattcagt ttgcttttgg
     8821 tgttgaaacc caaaagatca tttcagaagg tactcgctat cgtttagtcg gaaaagagac
     8881 ggaattagta gcagatatga ttttttgtgc gactgggaga cagccgaata cagaatcttt
     8941 agcacttgag caagcgaata tcgtctttga taaacacgga attgcagtca atgattatct
     9001 gcaaacgagc aatccaaaaa tttttgcgtg tggagatatt gtttcaagga aaaccccaaa
     9061 attgacacca gtcgccactt ttgaaggaaa ttatgtagct aagcggataa cggatgcaac
     9121 aagcgaacct atcaaatatc cgattatacc tacgatcgtc tacgccagtc ctaaacttgc
     9181 tgaagtagga gttacaaaaa gtcacgcctc ttcgtcagat caagtggtag agatggatct
     9241 taccagttgg ttcacctatc atcgtgtcaa tgaaccagtt gctaaggctg aattgacctt
     9301 tgaccagcaa aattatttga ttggtgctgc agtgattagc gagcaagcag acgaattgat
     9361 cgatgatttg acactcgtca tcaatcaaaa attgactaaa aaagagttgg atagctatat
     9421 catggggtat ccgacattgg caagtgatct ctcctattta ttgaaataat cagcatgata
     9481 actaaagcgc ttgttgcttt ttccttcttg tgctacactt taaaaaatga atacctgttt
     9541 ctggggatgt cgtagacgac atgagagtaa gttgtgaact tagacccatt gaacctgtca
     9601 gttaatactg gtgaagggag aaacatagcc gacgcttgaa gcgggttact atgtcgtgcc
     9661 catttcaagc gttttttgtt attcatatta gaagaaagga ggtaacaaaa tgaagaaaaa
     9721 tctttatcca gcagtcagta tggcattcat tttaattatt tggcagattg ttgtcatagt
     9781 ttggaaaata cctgccttta ttttacccgg acctgctgct gtattttctg cattagcggc
     9841 agatgcacaa gtattacttt cccattctgt cacgacatta aatgaagcag tgcttggttt
     9901 ggtaatagct agcgttcttt cttttattac tgccttggca atggattatt ggaagttttt
     9961 agaaatgtct ttttatccgt tactagtgat ttcacaaatg ttgccaatca tggtgttagg
    10021 gccattactt accctatggt ttggtttcgg tatgcttcca aaagtgatcc ttgttgtctt
    10081 gataagctat tttccgatcg tagtttcttt ttcggataca ttgaggaaag tatcaaaaga
    10141 gcaaatcatc tttttgaaaa cgatgggggc agatacaggg aagatttatc gaatatataa
    10201 aataccagcg ggtttatccg gcttcttttc cggtttaaaa gtggctgcta cttattgtgt
    10261 cagcggagca gtcgttggag aatggctaag cgctcaagca ggcttagggt attacatgat
    10321 acgcgtaaaa aacagctacc agatcgataa agtttttgct gcgatcattt gtgtcattct
    10381 attgagttta ttgctgaatg gcgcttgttc attattgaaa aaagggtatt atcgtatgct
    10441 ttatattttt taagagaagg ggatcaaatg aaaaaattat ggcttttatt accgctactg
    10501 ttgcttttat ccgcttgtgg cactgcaaaa gaaacaagta gcaaacaaga aataaaggat
    10561 ttgaaaaaag tgacacttgt tttggattat gtaccgaata ctaatcatac tggcatctac
    10621 ctagcaaaag aaaaaggcta ttataaagag gctggtttaa atgtccaaat catcgaacca
    10681 ggagataatt cgacaagtat tggtctagta ggtgcggaca aagcacaatt tggtgtaagt
    10741 tatcaagaag atgtcactta tgctcacgca gatggacaga atataccagt caaagcaatt
    10801 gctacagtga tcaaacataa tacttctgga ttcgccaccc tatcagacag taacatccat
    10861 tcaccaaaag attttgaagg aaaaacatat gcaggttggc aaagtccaag tgaagaagct
    10921 gtcttaaaag ctgtgatgga aaaagacggc ggggactttt caaaactgac aatggtagga
    10981 agcaatggag aagggccaga aagcttagga aaatcctcag atatccaatg gtactttgaa
    11041 ggttgggata tgatcaaagc aaaagaagcg ggtattgaac taaactatat cccattgaaa
    11101 gaattagatg aacggttaga ttactatact ccggtaatta ttacgaatga tcaactgatc
    11161 aaaagtgatc cagaattggt acagtctttt atggatgcca cgaaaaaagg ctatcaggaa
    11221 gctattaagg atccaaatga cagtgctaaa ctgttgcaaa aatatgcaaa agaaaatgat
    11281 cggacgtttt tagaagaatc acaagcattc ttgtcaaaaa attatacgga tgatcctgag
    11341 aattggggat tgatggaaga aaaagtttgg cgcaattata cagcttttat gcaggaaaac
    11401 ggattgatca agcaagatgt accttcgcaa gaactattca ctaatcagtt tataaaatag
    11461 gagtgataga tatgtcaatc caaatcagcc acttgaatgt agtcattgaa aacctgaaaa
    11521 tattagaaga catccatttc actatcccta aagatacctt tacatcgatt gtcgcgccaa
    11581 gcggagccgg taagtcaaca ttgctgaaaa ctttaacagg agtccagcct atgacttctg
    11641 gaacgatcaa agtagacgat caaaaagtaa ctggtctcca tacagcattt agttatatgc
    11701 cgcaagaaga tatgttactt ccttggctga gcgtctatca aaacgttacg ttatatcaaa
    11761 aaatcaatca tctaacaatc aatgaagaac aagtaaggga ttatctggat atctttgggt
    11821 taaacggcta tgaacatttt ttgccggaac aattatctgg cgggatgaag cagcgaacgg
    11881 ccttgttacg aacaatcatg aatccttctt cttacttgct tcttgatgaa ccatttggag
    11941 cgttagatgc tatgaccaga gggcagatgc aagactggat gttgaagctg cctaaaaaag
    12001 caaagcggac tactttactt gtgacgcatg atatcgaaga agcaatttat ttatcagaca
    12061 ggattcttgt gctttccgcc cgtccggcac atgtgattgc tgagattcaa gtgcctgaaa
    12121 aacgacggtc acgtgaatgg ctactgcagc aatcagagtt gaagcaaacg atttatcagt
    12181 tattggcagg tgaattagat gttaagtgat gctcattgtc atttagatgg gagtcagagt
    12241 ttagcactcc ttcagcagga acacacgatt ttgacgatca tcaattgtga ttcgccagaa
    12301 gaatggaagg aaaacagaca gcttgcagca agtaagactc aagccttaag ttacggtatc
    12361 catccttgga aagcagatag ctatacgttt gaacaagttg agcctttttt gaaaaaagca
    12421 aggatcatcg gtgaaatagg gttagataat atttggacga atgtaccgat gactacacaa
    12481 aaaaaagtat ttgagcgaca gcttgctttt gcagccataa atgagaaacc tgttgtactg
    12541 cacacaaaag gatgcgaaaa agagatctta aattatatcc aagcttaccc taatcactat
    12601 ttgatccatt ggtattcttc tttggactat caacaagact atattgattt aggctgttat
    12661 ttcagcattg gtcttgattt gaaaaagaat ccagcagtct ggcaactagc taaagcagtc
    12721 ccaatcgacc acttacttat tgaaacggat ggactacatg cagcagagtg ggctttaggg
    12781 gggatttttt cagaaaaaga ttatgtctca ctgcttgaag aacagttaaa gacaatcgct
    12841 gagatcaaag agctatcagt tgaagaagtt aaaaaaaata cttatcaaaa cttggaacgt
    12901 ttcattagat taggtaaata aaaaagagat gtgacacaac tagtgtcgca cctctttttg
    12961 gctttctttg tataaaagta aactgtttga ttaataaatg aatcttagta ccaaccgttt
    13021 gcttcccaga atgctttcgc cgcttcccaa gaaccataac gtgaagtaac atattgttct
    13081 gctactcttt cttggtttgc agcagagtag tcaccattta agtaagaaga atctaattgg
    13141 taacggccga tgtaacgtcc gttcgttgct gtgtaagaac cgcttgattc tttttgtgcg
    13201 atccattctt ttgcagagct tgtacttgct ggagtagcag cagtttctgt tacagcagga
    13261 gcaggttgtg tttcagcaac aggcgctgct tgtgtttccg tttgaaccgt ttcttgtaca
    13321 acaggttgtt cagcttgaac aggttcagct tggactggtg cttcttgaac ggctgcttga
    13381 ggttcagcag ctgcttgtgc agagccttct gttgggattg ttaattgttg tcctgagtag
    13441 atcaggttaa tatcagaaat tgaatttgat tctgcaatgg cattgatcaa ggagttatca
    13501 ccaacgtatt tttgagaaat cgttgataga gtatcacctg attgaacggt ataagcttcg
    13561 tccgcatgag cagtcgttcc catgaagaat gcagcgccgg cagccaaagt tgttccaaaa
    13621 agtaaagttt taagtgatgt catgaaaaat ctcctttatt cgttaaaaag ttttatttgt
    13681 tgtgttcaac gaggaatact ttaacacgtg aaaatatttc ataggtaaaa gaagcatgac
    13741 aatttatgac aaatccacgt tttttgtaac agaatgatgt tcgctcgatg tgaaaaatga
    13801 tgaggaattt cacataatgc agaaataatg ggattagaac aggtgttctg atgagaaaag
    13861 aaatgtataa ataacttgaa agaaattgct tttgttcatc attttattta catattgcgt
    13921 atattttttg tatttttgct tgagatgtta caataaaaga aaaaggaggc ggttccaatg
    13981 agtaaaagat accagaacat catggtagca gttgacggat caagacaatc gatcaaagcg
    14041 ttcgctgaag cccttgactt agcaaaggac aatgaagccc acttatttat cgtgtcaatc
    14101 atcaacaaag ttgagctgac ccatagtgcc tatgcctttt caaaaatcta tgcagatgaa
    14161 aaacaaagaa cagaagtagc catgctgaaa aaaatcaatg atgccaaaga gtacggcatt
    14221 gctgagatcc acgcaatagt tgaaactgga gatccaagaa atttgattgc caatgtcatc
    14281 cctggacaag aaaatattga tttgatcgtt atgggagcga caggaaaagg tgctatacaa
    14341 caagcattag ttggatcgac cgcttcttat gtcgtatcac atgccccttg cagtgtgttg
    14401 gtcgtgaagt aatgagataa gggaggaata gaaaatggtt cgtcctcaag aagtccgtgc
    14461 gccaaaagaa aaaatagaaa ttttagcaat tattgaagac ggcacacaaa caaagaaggg
    14521 atactcgatc gctttagtaa agtggaaagg aaaaaaaggt gttgctctga gatgggatgg
    14581 agataaccaa caagataaag gattccctgt cacagctaac ggttatcatc cagcgtggtt
    14641 tgttctgcct gataaattta tggaattata cagctatgat tatgcaagag ccgcagcatc
    14701 aatcaaagca ttagataaat taacagaaga gcagaatgaa atggaggaaa aataaaaaag
    14761 acgaaccaat cagcctttag aaaactgatt agttcgagga gtaaaaatga aaaaaggtta
    14821 tttgggatga aactagaata atgcgtataa cttaaataaa acttaaagca ataaaatggc
    14881 aagagggtga ggcaaatgga agcaggaaaa gtattacagg aagtaaaagc actatgtgca
    14941 gataaaaaat atgaagaagc gaaaagcctg atcgaaaaga acaaagaaaa tttggaagaa
    15001 aagtacccga tcgcacaaga atttatagaa ttaaaacaag gaacgatcct tcaccgattc
    15061 aaacattttt ttggtataac agaatagaaa aaacgattaa agatcggcag aaatttgctg
    15121 catctttaat cgttttcttt ttaaacatgc gagggattca attctgattc ttatataaaa
    15181 aatagatcca aataataaga aaaattactg aaagcgtcaa ttcgatgatg tgtgttatta
    15241 atacagacgt tgtttcccaa tgcctaaaag aaaaaattt
//
LOCUS       DAJSMI010000061        15266 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJSMI010000061 DAJSMI010000000
VERSION     DAJSMI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.061"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(101..481)
                     /gene="yajC"
                     /locus_tag="ON430_001471"
     CDS             complement(101..481)
                     /gene="yajC"
                     /locus_tag="ON430_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR3219395.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(578..1723)
                     /gene="tgt"
                     /locus_tag="ON430_001472"
     CDS             complement(578..1723)
                     /gene="tgt"
                     /locus_tag="ON430_001472"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR3219396.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1941..2639
                     /locus_tag="ON430_001473"
     CDS             1941..2639
                     /locus_tag="ON430_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR3219397.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2807..5446)
                     /locus_tag="ON430_001474"
     CDS             complement(2807..5446)
                     /locus_tag="ON430_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3219398.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6065..7096)
                     /gene="queA"
                     /locus_tag="ON430_001475"
     CDS             complement(6065..7096)
                     /gene="queA"
                     /locus_tag="ON430_001475"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR3219399.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7289..8464
                     /locus_tag="ON430_001476"
     CDS             7289..8464
                     /locus_tag="ON430_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3219400.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8468..9109
                     /locus_tag="ON430_001477"
     CDS             8468..9109
                     /locus_tag="ON430_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3219401.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9106..10023
                     /locus_tag="ON430_001478"
     CDS             9106..10023
                     /locus_tag="ON430_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3219402.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10020..10691
                     /locus_tag="ON430_001479"
     CDS             10020..10691
                     /locus_tag="ON430_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3219403.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11232..11720)
                     /locus_tag="ON430_001480"
     CDS             complement(11232..11720)
                     /locus_tag="ON430_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCR3219404.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11921..12187)
                     /locus_tag="ON430_001481"
     CDS             complement(11921..12187)
                     /locus_tag="ON430_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR3219405.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12187..12450)
                     /locus_tag="ON430_001482"
     CDS             complement(12187..12450)
                     /locus_tag="ON430_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3219406.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12926..13111
                     /locus_tag="ON430_001483"
     CDS             12926..13111
                     /locus_tag="ON430_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3219407.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13253..15076)
                     /locus_tag="ON430_001484"
     CDS             complement(13253..15076)
                     /locus_tag="ON430_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR3219408.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         4443 a         3264 c         2869 g         4690 t
ORIGIN      
        1 ttgagcaata tatcccatgc tcttatctct ttcttttttg aataaaaaat gaggtaggac
       61 atctttctgc cctacctcgt ctttaaataa gctttgatac ttatttttct tccttctctt
      121 ctgtctcttc cagcgtttct gttgtttcag tttttacttc aactgtctca gctggttctt
      181 ctgttgtttc aacagtagtc gttgcatcat ttgttacgac agtccctggt ttgaccgttt
      241 tgattgctgc acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca actgtccgtt
      301 tgtcattgtc gatttctgaa acaactccat gtaatccgcc gatcgttacg actgaatcac
      361 ctggtttcat gctatctaac aagttttgac gttcttgctg ttgtttcttt tgcgaacgtg
      421 acataaaaaa ccacattgct aatagcgcga taaacatcac aagcattggt aatgttccca
      481 tttttttcac ctcactaatt ctgtatctac aaaaaacact ttagcagaaa aatgctgata
      541 acactagttt tttacttaat ttttaaaaga ttttccttta gaagcttttt gcgttttctt
      601 tattgaaccc gtattcttca aaaaaggctt ctctaaattc aagcaaatta tcatccatga
      661 ttgcttgacg tacttgtttc attagattca acaagaagta taaattgtgg taagaagtca
      721 aacgaatccc aaatgtttca tcacatttga tcaaatggcg gatataggca cgtgtatagt
      781 ttttacatgt ataacaatca catttctcat caattggacg gaaatcatgg gcatatttcg
      841 catttttcac tactagacgg ccttgagaag tcatacatgt gccatttcga gcgattcgag
      901 taggcaagac acagtcaaac atgtctactc cacggataac accatcgatc aatgaatcag
      961 cagcaccgac acccatcaag taacgtggtt tgttttcggg gatcaacggt gtagtgaatt
     1021 ctaatacacg attcatttct gatttaggtt ccccgacaga taacccgccg attgagtaac
     1081 cagggaaatc catcccgact agatctttcg cgctttgacg acgaagatct tcaaaccctg
     1141 ctccttggat gattccaaat aatccttgac ggtctggatt agcatgagct ttcaatcctc
     1201 gttctgccca tcgagacgta cgttctactg attttttcac gtagtcgtaa ctttcatcga
     1261 atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc caatttattt tggatgttga
     1321 ttgctttttc tggtgataaa aacatacgcg aaccgttcaa gtgatttttg aaatgcacgc
     1381 cttcttcttc gatattccgc atatcactta atgaaaaaac ttgaaagccg cctgaatctg
     1441 ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc gccagcttct tctactaagt
     1501 cttcaccagg gcgtagccat aaatgatatg tattacttaa aataacgcca gcacccattt
     1561 ctttcaactc ttctggggac attgttttta ctgtagctaa tgttcctact ggcatgaaca
     1621 ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg ccagtatgtt
     1681 tttctttctt gattaaacga tagcgaatgg caggttgcgt cattcatttc cctactttct
     1741 atataaaata tcttgacaac tcccaccatc ataatcgttt caagtgcttt ttgcaagttc
     1801 cctcggcttc cttctacttt atttaatatt ctttttttta tccttttttt atgaataaca
     1861 aaaaaaagaa aaacattatg acagaaatat tttttgatac atggtatatt ggtgttatta
     1921 aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa gcaaaagaga
     1981 gtctaaaagg acgttgggga caagctgttt tactaaatct aatccctaca ttgattggta
     2041 ttgctactat tcttcttatt atgattccta ttatcttgtt tgccaccacc atatatgatg
     2101 gatcagcgag cgtctcagaa gcgacaactt caggaggcgg tagttcagga ggcggtggcg
     2161 gaattatttc cagcatcatt agcgctttgt tcatgagcgg tatttcttgg acttatcttg
     2221 atattttacg tggacaaaaa gatcagatcg aaccattcaa agatgcattt cgtggattca
     2281 aaggtttgtt tatcggcgga gtgatcagct tagctgtgct gatctcgatc tttacaacat
     2341 tatggggttt gctattagtt attcctggaa tcattaaaag ctattcttat tctcaaagct
     2401 attttattta ctacgatatc gtccaagaaa caggacaaac acctaaagta ctggatacga
     2461 tcactgcaag tcgaaaattg atggatggtt ataaaggaca acttttctgg ttagatcttt
     2521 catttatcgg ctggcatatc ttagcattag caactgctgg tattggttat ctatggctca
     2581 gcccatacat ttctgcaaca aaagcagcat tttatgaaca attgccaaaa gatatttaaa
     2641 agagagtgtg acttgtgacc gagggggtgg caggagaaca ccgtttatcg agaggcagta
     2701 aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca acattcatgt cacacctcct
     2761 ttttgtatag ttccagtaaa aagaaaatca acttagaaac agttaatcat tcctcttctt
     2821 ctgcttccgg taatcgatgg aacttgatca gttcattcac cgcgattggt acgatagata
     2881 aaatccctac caagaaccag tgattcaaac tgatgtgtac taggccgaat agttcttgcg
     2941 taaatggcag caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc atttctacca
     3001 aagctttatt ggtcgctaaa ttgactttga agatcgattg gctgctgcgt acattgaaca
     3061 catgcagaat cgatgaataa gctaagatca agaatgcaac agtttgaccg acatgctgac
     3121 ttgcgtctac ccatgggctg atagtatcga cataactacc taaataataa ccaaacaagg
     3181 ttacgatggt gaagacccct gcatttatgg caattttttg caataaacct cgagcaaaga
     3241 tcccttcatt tttcggaatg ggtggctgat ccatgatgcc gtgttccgct ggctctcttc
     3301 ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac aaacagtagt tgcacagctg
     3361 taaatggtgc tccccatcct aacagcatcg caatcaaaac aataaagatc tccgaaatat
     3421 tacagcttag caaaaagttg atagctttac gaatattctg ataaaccgaa cgtccttgac
     3481 tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa aatcatatct gcggcacttt
     3541 gcgccacatc tgttcctgtg atccccatgg cacacccaac gttacttgct ttcaacgctg
     3601 gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc acccgtacgt tgccaagatt
     3661 tgacgatccg gatcttgtct tctggcgtta cccgagcata gacagagaga gctttgacac
     3721 gcgcatctaa ttcttcgtct gataattgat gcagttctga tcctgaaagt gcttctgacg
     3781 gatcattcaa gatacctaat tctttcgcta tggcactggc agtcaccaca tgatctccag
     3841 tgatcatgac tgttttgatg ccagcctttt tcgctcgctt gattgctcct ttactttctg
     3901 gacgtggtgg atcgatcatt ccgatcagac caatcaaacg taagtctttt tccagtgctt
     3961 cagaagaaat atcttgcggt tcttcgtcat aagtagcata accaacagca atcactctca
     4021 gtgcccgttt accaaagcga tcattaacga tcgccgcttg ttctacatcg cctgaactaa
     4081 agcgcggagc aagaacatcg aatgctcctt tggtgattga gatgtatttt tcgcccattt
     4141 ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt actcgtggat
     4201 atttttcttc taattctgcc tttgtatgat aattctcttc aacagccctt acgatggcta
     4261 cttctgttgg attacctttg ataacaaggt cgtcatcttc ttgctcgacc gtcacatctg
     4321 tacatagtgc agccattttc aatatttcca tcgcgctgtt cgtcattgaa tcttcgatat
     4381 tcgttacttc atcttctttg ctccataccc ggcggacgcg catttcattt tgtgtcagcg
     4441 ttcctgtttt gtctgagcag ataacattag cggttcctag cgtttcaact gctggcaact
     4501 gtcggatgat cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt gtcaacgtaa
     4561 caataacagt caaggtttcc ggtacagctg caacagccaa ggaaatcgaa gtcatgaaca
     4621 tctcaaggag tggttctcct tgcagttcac caatcacaaa tactaaagcc gcagccgcta
     4681 aagcgatcat actgattcgt ttgcctagct gattcaatcg gacctgtaaa ggtgtttttt
     4741 gcatagtatt ttcgttcaaa agatccgcga tcttacccat ttctgtagcc atgccaatgg
     4801 cagtgacgat cgctttccca cgacctgcag caaccgtaca tcctttaaat accatattaa
     4861 gttggtcacc taatgactca tcttcttcag caataaatgc tgcatctttt tcaactgctt
     4921 cactttctcc agtcaacgcc gattcttcta cacgtaattg tgatgcttcg atcaaacggg
     4981 catcggcaga aatagcatca ccggactcta aaacaagaat atcacccact accagttgct
     5041 ctgactcaac agtggtcact ttgccgtctc gtatgacagt tgccgtttgt ttattcatat
     5101 cttgcaagga ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc aataccgagt
     5161 tgatcacaac gatagcgatg atcagcagtc cttcaaataa atccccatgt tctgtagcaa
     5221 atgcggtata aaaggaaatc accgctgcta caagaaggat gatcgttgta aaatcggaca
     5281 agcttcgaaa gaattttttg atcatcgatt ctttcggcgc ctcatcgaat ttattcaatc
     5341 cctgcttttt tcgccgttca tccacttctt gtgaagacaa cccagtatct ggatctgctt
     5401 ctgttacctt ctttacttct tctattgtct gcttgtattc ttccatccta ttcctccttt
     5461 aaaatcttcg tacaaaaaaa ggtacttatt ttactataac atcctttttt tatttacttc
     5521 aaataaaggg cttatcgatt atttgttaag aaagcgtaaa atttaaagtg cttatatttg
     5581 caaacgctac caaaggatgc tattatatag atgtaaggaa ggaggatacc atcaaataga
     5641 cagaagaatt tctcgataga acagtgtaag caaacaaagt tgttagaagt acaggtttca
     5701 cccagacatg cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga tgtttggatc
     5761 acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg taaacgtctg
     5821 aaaattgaat aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa acaaaatttt
     5881 tgtttaacat gaagaaattc aacaccgtgc tttgcatgta tgatggaaag ttacaatcat
     5941 ttctgtttat ttgaatcctt ccgaggtaca agaaagccga gcttatcgag agctcggctt
     6001 tttctttgtc ataaaatctt tgtaaagctt ttttgttatt caaaactagc ggcttttcat
     6061 aaccttattt cacgaacatc gcatctccaa aactaaagaa acgatagcgc tcttcaatcg
     6121 catgctggta agcagataaa gtcaattctc ttcctgcaaa ggcactaact aacatgacta
     6181 aagtcgattt tggcaaatgg aaattagttg agaaagcttc taccactttg aattcatagc
     6241 caggcgtaat gaaaatatca gtccatccgc tgtctgcttt gatttctcca ttaaatttcg
     6301 ttccaatcgt ttctaatgtt cgaattgaag ttgttccgac agccacgatt ttcccgccat
     6361 ttttacgtac ttggttcaat cgctcagccg cttcttccgt caaacggtag aattcactat
     6421 gcatatggtg ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct aaccctacat
     6481 gtagtgtcag atagactaat tcaacaccct ttgcttcgat ctcatcaagt aattctttcg
     6541 taaaatgcaa tccagctgtt ggcgctgcag cagaaccatt ttctttcgca tagacagttt
     6601 gatagcgatc tgggtcttcc agacgttctt tgatatatgg aggcaatggc atttctccca
     6661 atgattcaag aatctctaga aaaattcctt catactcaaa gtcaatgatc cgtccgccgt
     6721 gttccaattc ttctttcact actgcttgca atctgccatc accaaaagag attcttgtcc
     6781 caactttggc acgtttcgca ggtttgatca atgtctccca cgtatctcct tgtgtattcg
     6841 ttagaagaag gacttctaaa tgcgcaccag tatctggttt ttctccatat aaacgagcag
     6901 gcagaactcg tgtattattc ataactagcg catcaccagg ctgtaactct tcaatgattt
     6961 catggaaatg tttatcttcc atctctccag ttttccgatc caaaacaagt aaacgagaat
     7021 gatcgcggtc ttttaatggt gtttgtgcga ttaattcttc cggcaagtca aaatcaaaat
     7081 cttctgttgt taacatatcg tcactcactt tctatcatgt gcttcatcat tctatcatgt
     7141 ttttcgttct gttggcaaaa tcagcacttt ttcacttcta ttttttgttt tcctaaataa
     7201 aaaaactacc ctatttgtat tttttcattg ttttaggcat actagtaata gatagttctt
     7261 cattgaaaat agaaaaagag gtggcaccat gattgaattt caaaatgtgt caaaaattta
     7321 taaaggtgga aaagtcgctg tagaggacgt caatctttca tttgaaaagg gtgagttcat
     7381 ttgttttatc ggtacgagcg gaagcggaaa aacgacgacg atgcggatga tcaaccgaat
     7441 gactgatcca acacaaggga aaattttgat tgatggaaaa tccatccaag aaattgatcc
     7501 agtggaattg cgacgtcaga tcggctatgt tatccaaagt attggattga tgcctcacat
     7561 gacgatccgt gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc aagaagaacg
     7621 gaataaaaca gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga tgttagatcg
     7681 ctacccacat gaattatctg gtggtcagca gcagcgtatt ggagtcgttc gtgcccttgc
     7741 agcgaatcaa gatatcatct tgatggacga gccattcggt gcgcttgatc caatcactcg
     7801 tgattccttg caagaccttg tcaaagattt acaagagcgt ttgggaaaaa cgatcgtatt
     7861 tgtaacccat gacatggatg aagcgattaa actagcaagc cgtatcgcca tcatgagtga
     7921 aggccgtgtg atccaatttg atacaccaga aaatatttta cgtcatcctg caaacgattt
     7981 cgtagaagag ttgattggag aagatcgcct actacaagcc aagccaaatg cgacaagagt
     8041 cggcgaagtt atgctaaata cagcaatcac gattacaccg gaaaaatcat tacaagaagc
     8101 aatcaaactg atgagggaaa aacgtgtaga tacgttgtta gttaccgata attcgaatgt
     8161 attaaaagga tttatcgatg tagaaacgtt gaataaaaaa cgaggaaaag tatccagtgt
     8221 cggcgacatt ttgaataaac atgtttttta cgtaaaagaa tccgcctatc ttcgtgatac
     8281 cttgcaacgc attttaaaac gcgggctgaa atacgtgccg gtcgttgatg aacagaacaa
     8341 agtcgtaggt atcctgactc gtgcatcatt agtggatatc gtctacgatg tgatttgggg
     8401 agaagatccc gcaactactg atgttgcaga gtcagctact cctgaagaat ctaaggaggt
     8461 ctgacctatg cagcagtttt ttcatcagca cggttcagaa attctctcaa aaggttttga
     8521 acatctttat atttcaggac ttgctttgat cattggtatc atcattgctg taccgcttgg
     8581 tgtcttactg acacgttttc caaaagctgc cacgattgtg attggcttaa ccagtgcctt
     8641 gcagacaatc ccttctcttg ctttattggc attgatgatc ccgctatttg gcgtaggtaa
     8701 attacctgcg attatcgcac tctttattta ttcattattg ccgattttac gtaatacgta
     8761 cattgggatg aaaaatgtcg attggaatta tcgggatgtg gcaaaaggca tggggatgac
     8821 tgagttccaa tccatttttt ccgttgaatt gcccattgct atgccaacga tcatggctgg
     8881 tattcgttta gctgccgttt acgtgattgc atgggctaca cttgcctctt atatcggcgc
     8941 tggtggttta ggcgatttga tttttagtgg actgaataat taccaaccag atctcatttt
     9001 ggcaggtacg atccctgtaa caattttagc actattggct gattggcttc taggtctctt
     9061 ggaacaccgg ctgacaccga tcgcacttag agaggagaat gaagaatgaa acgtatacta
     9121 aaaatgacca tggttctttt ctctcttctt atacttgcga gttgttcctt ccctggtctt
     9181 gccagcaata cagatgaaga caccatttcg atcacaggtg gaatcacttc agaagcacag
     9241 atcttagcaa gtctagttgc cggaatggtt gaacattata cagataaaaa taccgcaatc
     9301 atcaacaatc tggctactac aactatcaac catcaagcga tgatgaacgg agatgcttca
     9361 atttcagcgg ctcgctacac aggaacagat ttaacgacta cattgaacct tcctcctgaa
     9421 aaagatccga aaaaagcgtt tgctaccgtg aaagatgaat ttgagaaaag atacggtcaa
     9481 acgtggttcc cgtcctatgg ttttgaaaat acgtatgtct ttcttgttcg aaaagacacc
     9541 gcacaaaaat accatctatc taaagtcagc gatctgaaaa atgtagcgga tgaattagta
     9601 gctggtgtag atacttcttg gatcaatcgt aaaggagacg gttatgacgg cttccaagaa
     9661 acttatggct tctccttcaa ttcgatatta cctatgcaga ttggacttgt ttatgacgca
     9721 gtcgaagcag gaaaaatgga tattgttctt ggctactcta cggatggacg gatcgccagt
     9781 tatgatctag tcatgttgga agatgatcgt caattcttcc caccatatga tgctgctcca
     9841 gttgtagaca acaagatatt agctgatacg cctcatttaa aagaagcctt aaataagtta
     9901 ggtcatacca tcagtacgga aaaaatgcag gaattgaact acgaagcaga taataatcta
     9961 gtggaaccat cagtagtagc acaacgtttc ttacaagaaa accactattt tgaagggaag
    10021 tgaagcaatt ggataaaatg aatgtctttc aacaattttt ctattatttc caggaaaatg
    10081 gctcctatat ctttgcacaa tttatccgtc acttcctgat ttctatctat ggtgtcctct
    10141 ttgcagcagt tgtcgggatt ccggttggga tcatgatttc taggagacgt aaattagcaa
    10201 actgggtgat tcgtttggcg aatatcatcc agacgatccc ttctctagcc atgatctcca
    10261 ttttaatcat tggattaggc ctgggagtca atgtcgtgat tgttactgta tttttgtatt
    10321 ccctcttacc gatcatcaag aatacgtata ccgggatgat ccaagtagac aaaaacatcc
    10381 ttgatgtcgg caaaggcatg gggatgactg ctagacaacg actattcatg gtagagttac
    10441 cactctctgt ttctgttatc atggcaggca taagaaacgc gttagttgtt gccatcggga
    10501 taaccgcgat cggtgcattc gtcggtgctg gtggtcttgg agatatcatt atccgaggaa
    10561 ccaatgcaac agagggcaca tcgattattt tagcaggtgc attgccaact gcattaatgg
    10621 cgatcatcac ggactggctt ttaggtattt tggaaagacg gctagatcca gccagccgta
    10681 catcacgctg atgttattgt aaaaaacaaa agcttctgcg accatttgtt ggtcgcaaag
    10741 gcttttttag ttctgtcatt tttaaggcaa taatccactc tgattcttcc ctccttatgg
    10801 gcaaagagtt gcttttctta gctatttatc cattccgtat actccaactt catactgcca
    10861 acgggttcat atctgcatcc accgactaag tttttttctt atccgctaag gcattttgcc
    10921 aatccatttt cccatcactc gtcatggaat tcataccaca tgatctctaa atactcgtct
    10981 gcttgttctt tgaattgggc atcaattctt acatcctttg gcgtggcttt catttaagcc
    11041 ttttccatca gtcctcgatt gttcgatgcc ttcagctgca tcatcaaaac tggtgactgc
    11101 cgtatcggta acttcgatca tcttttatac aatcgggaac ttgccatcct catccttggc
    11161 taaaaacata tctagccatt ccagcttctc gccatgggag gtttgcggtt cctaaaaccg
    11221 gtacacgatt cttatggact gcatccgtcg caactagact tggcgatacg atgatccttc
    11281 accagaagag cacccccgta aaccaattga tcgatatatt gccaataaga aaagacattg
    11341 gcgtaaaaga tatcgatccc tcgtgatgca ttgccgttga tactgctatt catggccgaa
    11401 agcgccacga ctttaacttc ttccgtttga gtgacattca tgttcggcaa tgtcgctttg
    11461 gatacccgct ctgctaaagg aacgacactg gtattgtatt tcgtatctgt atcttttcca
    11521 aaagaccatg ccaacgaatc ctctgggaac caataagacg actccggttg attgtccatt
    11581 gcttttttca acatactttt ggctgtttct cgataaacga catttccaat tgtcttcgcc
    11641 tcactttggc gataatcgat ggtcgttgcc actgccaaca ctaacaacga tgctccaatt
    11701 ttactgattt tccttgtcac acttcttcct ccttgtttta cataaacccg tcaacattcc
    11761 attcctaaac tcaaacaatg acccttcaaa ggtggagata taggaacaaa ttaaagaaat
    11821 catgcaataa atccaagaag gttggtttta gaacaaaaaa agagtctcct ttttggggag
    11881 actctttgtt aactatctaa aaaggttggc tcttctcact tcactcataa tgtgtccagg
    11941 cagaataaat ttctacaact ttattttcct catcaacttt gtaaaccact cgatgctgtc
    12001 tattcagcct gcgtgagtat ctgccttcat gtgtaggtct cagcttttca aaagactgcg
    12061 tgggcatata tggatcttcc ttcaatgtct gaatcacttc ttcaaattgt ttctttaaat
    12121 tcgtttgctt gatttttctt aaatccactt tcgcagagtt tttgattgcg actgtatagt
    12181 tactcatcaa agagcttccc aatcaatatc atcgatattc gtaaagctac tgtcgtcttt
    12241 ttcgcggtct cgtactttat ctaaagtacc tgtttgctca agaaaaaggg tttcttgaat
    12301 cgctctccag tcttccagcc caatcaatac agcattgttt ccactacgat cactaataat
    12361 ctcgatttca gtatgatttt catttacctc tttcaataac tgataaaaat ttttacgagc
    12421 agtgctagga ttcatcgttg tattagccat tgtctctcct cctttttctg atataattat
    12481 agcgtacttg attaagtacg tcaaaaaaat acaataaagc acttaaattc agcaaacttt
    12541 tcctcttttt tacatagaga aaaagacccc tttttaggga ggatctttgt ccacgctcac
    12601 ttcttactcc tttacgacta gcggtagcca aacgtaaagt aattggtaac aacgtatcat
    12661 ctttagcaaa ggtcgaatcc aaagcctgtg agtctatgca acaacaccaa tacacgaagg
    12721 ttcctcgttg cagtgtgact tgattgatat cttgaataac ctgaggatac gcacgaaaga
    12781 tttgtaacgg ttattcaaaa ctgattttgg ccgtcaagtc ttctactgcc tcaaaaatga
    12841 ttgcctcttt cgtctcctgt cattcatgtt cactatcgat ttgatacttg cttagaccgt
    12901 tgggtttgtg aatcaccaat taaaaatggc actgagttat ggtaacaaaa aatggtcact
    12961 tgacgaggct gagattccta cttatgaaga cacatggcac ttaagagtcg aggtaaagga
    13021 agaagctgaa aatataggag gctgtacatt atttggcaaa ggattcggaa attatgatca
    13081 agacatcaaa atcaaaatgt tttacagcta aataggaaga ataataacat ttcatacgct
    13141 gttcccatct ttaatggctt atagagggca cctttagctg agaataagac aaaacagacc
    13201 ctctagcttc aattaagagc tagagggtct gtttttgatt ttaaaacttg gtttatttct
    13261 tcaaatggta gctataggtg ctgacaacga tattttcacg ccaagacaat cgcaatctta
    13321 accgcaaact tgtttggtta tggagaatat tctgccagcg tctgtttggt acaaattgag
    13381 gaatcaacac agtagttgta tagttttgtt tcgctgcatt tttacttacg atatctacat
    13441 aacggataat tggattttca atcgagcgat aagaagagtg gacgattgaa aaacggacat
    13501 ctgggaaatg ctttttaaat tctgcttcga tttctttttc tttttcgaca tcttcgtcta
    13561 gcgaaacatg catcgcaacg acataatcac cgatcgaacg tgcgtaattc aacgcaccga
    13621 tatttacccg tgtcacattc ccgactaata caagaacggt attcccgtca taatcatgta
    13681 gttttgcatc attttctaaa cgaagctgtt ctgctacttt tttataatga tcatggatct
    13741 tataaaaaat gaacataacg attggcataa tgatgaagaa aggccaaatg tcccctaacc
    13801 gatagacaaa caaaatcgca atgattgcat atgagatgaa cgcacccgta atattggcaa
    13861 tcgatttgct taaccatctt tttccttctt ttttccattt gacgaccatc cctgtttgag
    13921 ataaagcaaa tggaatgaac acgccgatag agtataacgg aatcaagcgt tctgttgagc
    13981 catggaaaat aaacagcaat acgatcgaac ctagcgctag tgtgataatc ccattggaat
    14041 agcctagtcg atccccacga tcttgataca tgtgaggcat gaatttatct tttgccagat
    14101 tgtatgcaag aactggaaaa gcagaaaaac cggtgttagc agcaactgct aaaatcagcg
    14161 ctgtagcaaa ttgaaggacg taatacaaaa taccatgtcc aaagacagct ttaccaattt
    14221 gagacaaaac agtgacttct ttttcaggaa cgatcccata ccaatagttg ataaatgtga
    14281 tccccacgaa gaagaaacct aagatcaatg ccatcatcgt caatgtagca gcggcatttt
    14341 ttgctcttgg cttcttaaag aatggtacag cattactaat cgcctccacc ccagttaaag
    14401 aggaagaacc agaagaaaat gctcgtaaaa tcaaagcgat agaaacaccc ggcacagccg
    14461 ctcctggaag tgcagtagct tgcaatggca cagcacctgt cacgatatta tataaaccaa
    14521 caacgatcag taacgagatc acaaggataa atgtgtacac cggaaataat aggaacgatg
    14581 cagattccct cagcccacga agattcagca tcatcagtag caaaacgatt gtcacagaaa
    14641 tagcgacttg atgaccgtag agtgctggca ccgctgatgt gatcgcttca gcacctgctg
    14701 agacagatac tgctactgtc aacatgtagt cgatcaataa agagccaccg gaaatcaagc
    14761 cagcattctt tccaagattc tcactgctga cgacatatgc tcccccgcca tgaggataag
    14821 catggatgat ttgacgatat gatagcgtta atgaaatcaa taaaataata acaaaagcgg
    14881 caatgggtag cgaataccag atggctgcag cagacaaagc gactaaaacg acaacgattt
    14941 gttccgtacc atatgcaata gatgataacg catcagacga caataatgcc aaagcagcaa
    15001 atcgggttaa tttgtgctca tcattttcag cagattttaa aggcttacct actaacaacc
    15061 tttttaagta atccaatttc taaactcctt tacacacaat tttacttcaa gttttatagt
    15121 cgaaaattta tagtaaacaa gatgttatag tacgctcact gaccttaaaa gtcaataaac
    15181 tttaagaaaa taattggaaa aaaacagaaa aatatcaagc gctttttcaa acgctttaat
    15241 acagcaaaaa tctaacattt ttttat
//
LOCUS       DAJSMI010000062        14548 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJSMI010000062 DAJSMI010000000
VERSION     DAJSMI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.062"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(108..302)
                     /gene="ssrS"
                     /locus_tag="ON430_001485"
     ncRNA           complement(108..302)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ON430_001485"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(374..1660)
                     /locus_tag="ON430_001486"
     CDS             complement(374..1660)
                     /locus_tag="ON430_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCR3219409.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            1798..2469
                     /locus_tag="ON430_001487"
     CDS             1798..2469
                     /locus_tag="ON430_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCR3219410.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            2435..2923
                     /locus_tag="ON430_001488"
     CDS             2435..2923
                     /locus_tag="ON430_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCR3219411.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            2936..3883
                     /gene="prmA"
                     /locus_tag="ON430_001489"
     CDS             2936..3883
                     /gene="prmA"
                     /locus_tag="ON430_001489"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR3219412.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            3884..4648
                     /locus_tag="ON430_001490"
     CDS             3884..4648
                     /locus_tag="ON430_001490"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR3219413.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            4881..7094
                     /locus_tag="ON430_001491"
     CDS             4881..7094
                     /locus_tag="ON430_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR3219414.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            7105..7551
                     /locus_tag="ON430_001492"
     CDS             7105..7551
                     /locus_tag="ON430_001492"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR3219415.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            7722..8981
                     /locus_tag="ON430_001493"
     CDS             7722..8981
                     /locus_tag="ON430_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCR3219416.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9070..10017)
                     /locus_tag="ON430_001494"
     CDS             complement(9070..10017)
                     /locus_tag="ON430_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3219417.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            10411..11703
                     /locus_tag="ON430_001495"
     CDS             10411..11703
                     /locus_tag="ON430_001495"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR3219418.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            11718..13487
                     /gene="aspS"
                     /locus_tag="ON430_001496"
     CDS             11718..13487
                     /gene="aspS"
                     /locus_tag="ON430_001496"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR3219419.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            13596..14471
                     /locus_tag="ON430_001497"
     CDS             13596..14471
                     /locus_tag="ON430_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3219420.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
BASE COUNT         4764 a         2631 c         3164 g         3989 t
ORIGIN      
        1 agtggcgata atcattaaaa tcctaatcat gaacaaatcc tccaaaataa gcaacaaata
       61 gaaaaagcaa tttgccaaga gcaaattgct ttattcttta aaagatggta atctccgtta
      121 atgccgttgt ttgtcctgta gtattgatcc cgcgaattca agcaggtggg tcccatggtc
      181 gtagcttcga actaccaaat gataaggcat gttaccagca gtgacacaag acgggtccca
      241 agatatcaat aaaaatgttc ggtcaacaca caaagaggac aattcgaaca tcacagattt
      301 cccacctcta tagtagcata tgaatttaaa aagagcaatg agagagctcg cagaaacaga
      361 accgttttct tgtctagttt tttggaggat gctttttcca ctcttttata cggtaatact
      421 gttgacccag agcctgttca tatttccctg tatcttttgg ctgataatac tgagcattct
      481 tcagtttatc tggtaaatat tgctgattga cccaagcctg atcaaaatga tgaggatact
      541 gataacccac gccacggttg agtgatttcg cacctttgta atggctatct ctcaaatgat
      601 caggaacatc tccagctttg ccctctcgaa tatcagcaag tgctgcatct aatgccatgt
      661 aggcagaatt ggattttggc gacaagcaaa gatcgacgac gacgtctgct agtgggatac
      721 gtgcctcagg caatcccaat ttttcagcag ctaatacagc gttcactgta cgagccgcag
      781 cagcaggatt acctaaacct atatcttcat aaccgataac catcagacgc cggcaaatac
      841 ttgccagatc acctgcctca actaaacggg cgagataatg aagtgcagca tccacatcac
      901 ttccacgaat cgatttttga aaagccgaaa taacgtcata atgagcatct ccatttttgt
      961 catgggtaag agcttttctt tggacacatt cttcaatgat cgacagtgta agatgaatcc
     1021 ttccttcttt atctgaagga gtcgatagtg tagccagttc cagcccattc aatgcactcc
     1081 gcaaatcgcc gttcgttgca cgcgaaaggt gaagaagagc atcttcgtct aactggattg
     1141 cttgttgtcc aaggccgcgt tctttatccc ttaaagcatg ttcgactgcc aactggatgt
     1201 cttgttctgt taatggtttg acttcaaaga tctgtgtacg gctgcggatc gctggattga
     1261 tggtaatata aggattctca gtagtcgctc cgatcaagat gattcggcca ctttctaaat
     1321 gaggaagcag aaaatcctgt tttgttttat ctaatcggtg tacttcatcc aataagagga
     1381 tcactgtacc actcattttt gcttcttccg ctacaacttg tagatccttt tttgaatctg
     1441 tagcggcgtt tagcatgcga aatgcataat tagtagagcc ggcgatcgca cttgcgatac
     1501 tcgtttttcc agtaccagga ggtccatata gaatcatgga agagagcatg cgagcatcga
     1561 ccatgcggcg tatgattttt ccttccccga ctagatgctg ctgaccaacg acttcatcga
     1621 tcgttcgagg tctcatgcga taagccaatg gctgttgcat aggattttcc tccttcttgt
     1681 ttttacgtcc cttaattata tcatatttag caagatgcct agaaagaaca gccttctgtt
     1741 gttttgtttt catagcttat ccaactaata ttgtgtatga taaagtaggt gagaaaaatg
     1801 acggataaac aaaagataga tgaatttttt tggaataaaa cgacgccaga agtggcacgg
     1861 gatttattgg ggatgtattt ggagcatgac acaccagaag gaagattagc tggatatatt
     1921 gtagacgcgg aagcatacct aggtccagaa gacgaggccg cccatagcta tggtttaaga
     1981 agaacaccgc gtgtgcgggc aatgtatgaa aaaccaggta cgatctacct ttacacgatg
     2041 catactcatc ggattttgaa catcattact cagccagaag ggattcctca aggagtaatg
     2101 atacgtgcaa tcgaacccgc cgcaatgatt gatcaaatga gtaaaaatcg tggcggaaaa
     2161 actggtccag atatcagcaa cggtccggga aaattagtgg aagcattagc aattcctcaa
     2221 gaactttatg gtcagtctat agcagatagt tcgttacgct tagtttttga gaaaaaaaag
     2281 acaccaaaaa agatcaatgc tttgcctaga atcggtatcc caaacaaagg tgtatggaca
     2341 gaaaagccgt tgcgttttgt tgtgtcggga aatccttata tctctttaca aagaaaaaac
     2401 caaatcgaga aagattgggg ttggaggaaa gaaaatgaaa aagaaggatc tattaacata
     2461 tttagatgaa ataattgaaa aacaagtgac agactatgat gtagctttag attgggatac
     2521 aaaaaatcat acgatcgaga tcgttgttcg cttgtttgca gaaaataaag cacaatttga
     2581 gattgatgat gcagaaggga tcgtttcgca agaaccaatt atcgaatttg aagacggcgt
     2641 acttcttttt aatcctcaga aatctgtttt tgatgaacgc aattatctag cagtaatccc
     2701 ttatgaagga aaaaaagggt tggcaaaatc agtagctgat tctttagtgg aatatctaaa
     2761 tgaagtatta gctcaagggc aaagcgattt gcttgatttt ttggatgaag acgatgacga
     2821 agctgtattc gagcttcact gggataatca gcaactagca gaattagtag aagtaaaaca
     2881 gcaaaatgaa gcagatactt atttaccgta cccaagttat taaggagtga cagatatgaa
     2941 atggaatgaa gtaaaagtag aaactgaaag tgaagcagtc gaagcagttg ctaatatttt
     3001 gatggaggca ggggcgagtg gtgtcgcgat tgaagattct ttagatgttg aacattttca
     3061 gtcggatcaa tatggtgaga ttttagataa aaatgatttt acatcgctga aagaaggcgc
     3121 actagtcatg gcttattttc cggaaacgac tttcttaccg gaaatcttgc cttttatcaa
     3181 agatcgtatc acacaattac ctgagtttgg tctagctatt ggaaaaaata aagtaacagt
     3241 tagtgaagta gaggaaagca actgggcaac agcctggaag aaatactatc atccggtgcg
     3301 tattacgcga atgctgacgg ttgcgccaag ctgggaaaac taccagacag aagatcctct
     3361 agaaaaaatt atcacactag atcctggtat ggcatttggc acaggcactc atcccacgac
     3421 ttccttgaca ttgacagcac tagaaacagt cttgcgtggt ggtgaaacat tgctagatgt
     3481 gggaactggg tcgggcgtgc taagtattgc agcaaaatat cttggtgcaa aagatgtcta
     3541 tgcctttgat ttagacgaag tagccgttcg ttcggcgaaa gaaaatatgg atatgaatga
     3601 aatagccaaa gatgtccatg tatcagctaa cgatcttttg acaggaatcg agattgaaag
     3661 tgatgtaatc gttgcgaaca ttttagcaga tatcatctta ttgatgatcc cagatgcttg
     3721 gcgtttactg aagcaaacag gcacgctgat cgtttcagga atcattgaag agaagaagca
     3781 aatggttctg gatgccatgt tagaacaagg gtttatcgtg gatcaaattt tccagcaaaa
     3841 agactggtat gcaattattt tgaaaaaacc agaggtggaa tagatgcagc ggtatttttt
     3901 aaaagaatca tatgaatcaa aagactattt tgttttagaa ggggaaaact atcaccacat
     3961 tgtacgggtt atgaggatgg aacccgagga tgaagttttt ttggcattca atgatcaaat
     4021 agccattgtt gcagaaatca cggagattac tgaagaggct gtttatctga aagaaattaa
     4081 aaaagaagaa aatcaaaagg aactgccaag ttcgattacc atagcgagtg gctatcctaa
     4141 aggggaaaag ctggattgga tcgttcagaa atccactgaa ttaggagcgc aagcttttct
     4201 tggtttcccg gcatcagcct ctgtcgttaa atgggatgca aaaaaacgta tgaaacgaca
     4261 acaacggctt gaaaagattg ctcaagaggc agcggaacaa tcccatcggc agatcgtgcc
     4321 atcagtagag ttattggaaa cgaaacaaga tttatttgag cggctagatg attatgacgt
     4381 ggtcttggtt gcttatgagg aatcagcaaa gcaaggcgaa aaatcaaatg cagccaaaat
     4441 tcttagtgaa acaaaaccag atagtcgaat cttagctgtc tttggaccag aaggtggatt
     4501 atcccgcgat gaagtcgatt attttgtaga acacaaaggg atactttgtg gattaggacc
     4561 aagaatcctt cgtacagaaa cagcacctct ttatttatta agtgctgcta gctatcaatg
     4621 ggaattaatg aatccttctt tcagctaacg ttcttcatta ataaaagagt atcgacagaa
     4681 agaattgaaa aaatagtcaa caatttgtat aatagaaaca attatttata caatggatac
     4741 gaaaaatcgt ttgaaaaagc ttttaaaaag cgaacatttc cgtaaaaaaa cgtataactg
     4801 cacggatttt gggacaatta tcaaacagat atttgtcccg attcccgttt taccgaaaaa
     4861 agaaaggggg atcgattcct gtgacaaaag aagaaatttt aacgggaccc ggtgtcataa
     4921 agctcgttag tcaatacatg ggtcccgaac atgtagcatt tgttgagaaa gcatgcgaat
     4981 acgcgactgc tgcacatgac ggacagttta gaaaatcagg cgaaccctac attattcatc
     5041 ctatccaagt cgcaggtata ttagcagatt taaaaatgga tccccataca gtggctacag
     5101 gcttcttaca tgatgttgtt gaagatacag aaatcacttt agaagatctg agagaagaat
     5161 ttggcgatga cgttgctatg ttagtggacg gcgtaaccaa attagggaaa ataaaatata
     5221 aatcccacga agagcagcta gcagaaaacc accgaaagat gctgctagca atggctcaag
     5281 atttacgagt tatcatggtc aaattagctg atagactaca taacatgcgt acgttgaagc
     5341 acctgcgaga agataagcaa agaaggatcg ctcaggaaac tttagaaatc tatgcgcctc
     5401 ttgcacatcg tctagggatc agccggatca aatgggaatt agaagataca gcacttcgtt
     5461 atctaaatcc aaaacagtat taccgtatcg tccacttgat gcagacgaag agagaagaac
     5521 gtgaaaaata cgtaagtggt actgttgaag atattcgaat agcgacagaa gagctgggga
     5581 tttttgcaga aatctatgga cggccaaaac acatttattc aatctatcgt aaaatgaaag
     5641 atcagaaaaa gcagttcaac gaaatttatg acttgctagc tattcgagtg atcgtagatt
     5701 caatcaagga ttgttatgct gtattaggag caatccacac aaaatggaaa ccaatgcccg
     5761 gcagatttaa ggattatatt gccatgccaa aagctaatat gtaccaatct ttgcatacta
     5821 ccgtgatcgg tccggcagga aatccggtag aaattcaaat ccgaacacag gaaatgcatg
     5881 aaatcgctga attcggggtt gctgcacact gggcctataa agaaggaaaa aatgaaaaag
     5941 tagaaccaga tggtatgacg aaacaattaa gctggttcca tgagatactc gaacttcaag
     6001 acgaaagcta tgatgcttct gaatttatgg aaggcgtaaa aggagatatc tttagtgata
     6061 aagtctacgt cttcacacca aaaggagacg ttactgagtt accaaaagga tccggaccat
     6121 tggactttgc atacagtatc catacagata tcggtaacaa aaccactggt gcaaaagtaa
     6181 atggcaaaat ggtgcagctt gattacaaat tgaaaaacgg agatatcatt gagatcatga
     6241 cttctccaaa ttcatttggc ccaagtcgcg actggttgaa attagttgct actagcaaag
     6301 caagaaataa gatcaaacgt ttcttcaaag cccaagatcg agaagaaaat gtgatcaaag
     6361 gccacgaatc cgtggtcaaa tgtattacag atctaggatt tacgcctaaa gatattttga
     6421 cgaagaacaa actgcaagaa gcactcgatc gttttaatta tcaaacagaa gatgatctct
     6481 atgcagctgt agggtatgga gaagttagcc ccttgacgat ggccaatcgt ctgactgaaa
     6541 aagaacgtaa agaacaaaaa atcgagcagc aaaagcaaga agcagaagaa atcatgaatc
     6601 agccgaaaaa agaacctgac aaaatgaaag tacgtcatga aggtggcgtt gtcattcaag
     6661 gggtagaaaa cttactaatt cgtatcagtc gctgctgtaa tccgattcct ggtgatgata
     6721 tcgttggtta tatcactaaa ggcagaggga tatccattca tcgtcgagat tgtccgaatg
     6781 ttcagcctga caaaccaaat gtagcagaac gtttgattga agtcgaatgg gaagatacat
     6841 cgaatacacg aaaagagtat gatgcagatt tggaaattta cggctataat cgttcaggct
     6901 tattgaatga tgtacttcaa acagtcaatg cgctaacgaa aaatctcaac agcgttgaag
     6961 cacggacgaa taaagataaa atggcgacga tccatttgac ggttggtatc cagaatttat
     7021 cccacctaaa gagtatcgtg gataaaatca aagcagtacc tgatgtctac agtgtacgcc
     7081 ggacgaatgg ataggaggac agatatgaga gcagttgttc aacgggtaag taaagctgct
     7141 gtgtcagtcg atcagcaaga aattggaaaa attggctacg gcctacttgt tttattaggc
     7201 attcatgaaa ccgatacgca aaaagatgtg gactatctgg taaaaaaaat tgcacaaatg
     7261 cgtatttttg aagatgaaca aggaaaaatg aatattagtg tagaagatgt gggaggacaa
     7321 attttaagta tctctcaatt cacactgttt gcggatacga aaaaaggaaa tcgaccaagc
     7381 tttgtggaag ccgcacgacc agaaacagct attcctttat acgaatcatt caatgaagga
     7441 atcagaagtc gcggcatcac agtcgctacc ggacaattcg gagcagatat gtcggtttct
     7501 ctcgtcaatg atggacctgt aacgatcatt attgattcta aaaacaaata aagtaatctg
     7561 gttttcacta gtaaaaacta tgaaacacgt cgcttaagtt agactgataa actctaactt
     7621 aagtggcttt tttgtctgca aaaagcgctt cttttgtcag cagtcggaaa aaaagttaag
     7681 cttaaattat acaagttatt ttttatagga ggttgctgaa gatgaaagaa gtaccagtag
     7741 tagatatcaa aatcacagat ttaaaaaaat tgtatcaaaa aggatacaac atgttggagg
     7801 agttacgcca tgaagcggac gctcccgtcg tcaaagcaaa gattttcaat aaagaagcta
     7861 tcacgatcta cggatcatca gcagcaaaag tattttatga tccaaggaat ttcaaacgaa
     7921 aaggtgctat gccaaaatta gtattgaaga cactttttgg tcaaggtgga gtacagactt
     7981 tggatggagc tgctcatcac catcggaaaa atatttttat ggatctaatg acaccggaaa
     8041 gaatggaaga ttatcatcgt atcttagata agaatctgac acaagcactt gaagcacagc
     8101 atggtcaatt tgaactattt gatttaagta agatggtttt ctttacatct atttgcgagt
     8161 gggcaggtat caatttatca gcgatttcta aagatgaggt cgaaaaatta gcagaatatc
     8221 aaatctctat gatcagcgga acgtttactt ctccgattga tcacataaaa ggtgtcgaaa
     8281 atcgcaaaaa atcagaaaaa tgggcgcaag gcttgattga agaagctcga caaaatcctg
     8341 tggctggaaa agaaaatgtg gcattatatg cttttgccaa tgccacagat ttagatggtc
     8401 agcttttacc tctggaagtg gcagctgttg aattattgaa catcatccgt ccaacggttg
     8461 ctctaactgt gtgggcggca ttgatgggac atgcactttt cagtagacca gatttgtatc
     8521 agcaattaaa aaatgacttt tcgacgttac aagatccgtt tattcaagaa atgagacgct
     8581 attatccttt cttcccaatg ctacctgcga tttcgctgaa agaagtagaa gtagatggtt
     8641 atcgtatacc agaaggcagt tgggttattt tagatttata tggaacagac catgatgaac
     8701 gaacagttga agcgccagat tcctttatga tcaaacggta tgtagggaaa gcaaaagata
     8761 tttcctataa agaagaatat gagatgattg cacaaggcgg gggaaatttt cgacaaatgc
     8821 atcgctgtgc aggagaatgg atcactcttc atagtctacg tgtgttttcc gatcagctag
     8881 taaataagtt tgagttctct gtacctgaac aagattggac gattccattc aatcagtttc
     8941 cgacttatcc gaatagccga gcgctacttt acaagaatta gattagcata aacttatcga
     9001 aaaaaaggaa gatcaaacag tgctgatctt ccttttttca tattactata aattgataag
     9061 gtcaattgat caaaatccac gaaatgcttc atcaatggtt tgataatctt cattggataa
     9121 atgcaaatct aaagcttgga cattgtcaga tacttgctct ggacgcttag ctccaggtat
     9181 aactacagac actcgtggat tcttcatgta ccaagctaaa acaagttgcg caggagtggc
     9241 ttggtatctt ttagcaatgg ggcgaagtac atcgacagcg gttaaaattt ctctgaaacg
     9301 ttctccttgg aaatctggat ttctttttcg aggatctcct tcaccaaatt gtttttcctc
     9361 accaagttca tattttcctg tcagcaaacc ggaagcaaga ggaaaatagg gaacaaaact
     9421 gattttgttt ttttcaagat aagggaataa ttctttttca gcctgccgat ggatcaaact
     9481 gtatttatct tctactacat cgacatatcc atctgcattc gcttctttca attgttctaa
     9541 cgtgaagttt gacacaccga ctgctcggat ttttcctgct tcttttaatt cgtgcaatgt
     9601 agcaactgat tcattttttg gtgtgctttc atccggaaag tggatataaa aaatgtcgat
     9661 gtaatcagtt tgcagacgtt ttagtgcatc ttccaccgat tgtttgagaa actcaggaga
     9721 attatcaaat gttcgccctt tatttggtac atgagcagct tttgtagcga tgacgacacg
     9781 tgagcgatca tattcttttt ccttcagtac ctcgccaatc agttcttcgg aacggccgtt
     9841 tccgtaagca taagcggtat cgatcaaatt gatcccgcta ttcaaagctg tacgaactac
     9901 ttgttttcct gtctcatctt ccaaaccgtc aaaaagatta tgtcctccaa cggcatttgc
     9961 tcctaatcca agctgttctg cgtagacttg tgaatgtccg attcttattt ctgccatagc
    10021 tgattcctcc tttttgtcta gtctaaaaca aaataccaaa aagctgaact aaaacgtttt
    10081 tgtgagggga aatagtcaga tatctcttga ctttgagact ggtttttagt aggttataag
    10141 taatatcgaa tgtcaatgaa agagcgagta agtttttgaa actttacaag agaggatcat
    10201 cgaggctgaa aatgatctaa agtttcatta gcctaaagac actctggaga tggacgagtg
    10261 aaaagccgtc ccgcgtacga gcgttattcg taagaggaaa gagaaatctt tcaaaattag
    10321 gtggtaccgt gtatgaattt cattcaccct tgttcatgat gaacaagggt gattttttta
    10381 tttacatagc taagaaaggg agagaaaact atgagtttcc aaagaccaaa aggaacaaat
    10441 gatattttgc caggcgaatc agaaaaatgg cagtttgtcg aagaaacagc acgtttatta
    10501 ttcagagatt atcagtataa tgaaatgcgt acaccgattt ttgaacacta tgaagtgata
    10561 tcccgaagtg taggggatac aacggatatc gtttcaaaag agatgtatga tttttatgac
    10621 aaaggggaac gccatgtgac gcttcgacca gaaggtacag caccaatcgt tcgttctttt
    10681 gttgagcata aattgttcgg cccagagtat gcgaaaccgt tcaaaacttt ttatatggga
    10741 ccaatgtttc gctacgaacg ccctcaaaaa ggacggttac gtcaattcca tcaaatcgga
    10801 gtagaagcat ttggatcaga aaaccctgca actgatgtgg agagcatggt gatggcactt
    10861 gatttcttca aacagttggg gatcacgcaa atccggctag tcatcaattc attaggtgat
    10921 aaagaaacgc gtcaagccta tcgtcaagca ttgatcgact atcttttacc ttttgaatca
    10981 gagctgagtg aagactctaa acgccgcctt catgaaaatc cattacgcgt attggacagt
    11041 aaagacaaac gagatcaaaa atttgtggca gaagctccat ctatcttaga ttatctaagc
    11101 gagcctgctc aaaaacattt tgataccgtt gtttccatgc tggatgcatt ggctgttcct
    11161 tatgaaatcg acagtaatat ggttcgcgga ctagattatt acacacatac gatttttgaa
    11221 atcatgagtg acgcaccaaa aatgggggca caagcaacga tttgcgcagg cggacgttat
    11281 gacaatctag tggaagaact aggtggtcct tcaacacctg gcttcggatt tgcaatgggt
    11341 atcgagcgat tactcttgac tatggaggca gaggaagtag ttatccctgc atttaatgag
    11401 ttggatgctt acgtggtcgc actcggagac gaaacaaata tcgaagcatt gaaagtggtc
    11461 caagcgattc gaaactttgg tttttcagct gaccgcgact tcatgaaccg taaagcaaaa
    11521 gcgcaattta agacagcaga taaagcgaat gccaaattag tattggtgat cggtgaagat
    11581 gagcttgcta gtcaaacgat taatgtcaaa gcaatggcaa gccgaaaaga aaaaagtttt
    11641 ccattagaag agatatatga acattttgat gaagtctatg atgaaatgac tttattcgag
    11701 taggaggaaa aaaataaatg gcacaaagaa cgatctattg cggattagtt tccgaacaac
    11761 aattagaaca aacagttaca ttaaaaggat gggtacaaaa aagacgagac cttgggggag
    11821 taatctttat cgacctacgt gatcgtgaag gaatcgttca aatcgtcttt aatcctgaaa
    11881 tcaacaaaga agcatgggaa atcgcggata aatgtcgcag tgaatacgtg atcgaagtga
    11941 ctggggaagt tagaagacgc ggagaaaatg cagtaaatcc taaaatggcc actggtgaat
    12001 tcgaagtgat tgcaagtgaa atcgtgattt tgaatcaagc aaaaaccccg ccatttcaaa
    12061 ttgaagatga tcaagcaatc agtgacgaaa tacgtttgaa atatcgttat ttggatctgc
    12121 gtcgtccaaa aatgactaaa aacatcaaat taagaaatgc gacaacaaaa gcgattcgtc
    12181 attatttaga tgataatgac tttctcgata tcgaaacacc ttacctaggg aaatctacac
    12241 cagaaggcgc aagagactac ctagttcctt cacgtgtcca tgctggacat ttctatgcgt
    12301 tgccgcaatc tcctcaattg tttaaacaat tgctgatgaa tgctggattc gaccgttatt
    12361 atcaaatcgt tcgttgtttc cgcgatgaag atttacgtgg agaccgtcag cctgaattta
    12421 cgcaagtaga tattgaaaca acgttcttaa cagcagaaga aatccaaact tacacagagg
    12481 gattgattgc gaaggtaatg aaagaagttc gcggtatcga agtcacattg ccatttcctc
    12541 gtatgaccta tgatgaagca atggctcgct acggcagcga caaaccggat acacgttttg
    12601 atatggaatt gatcgatcta agcgatacag tcaaagaagt agaattcaaa gtcttccaaa
    12661 tggctcttga aaacggcggt gtcgtgaaag cattgaatgc taaaggcgct gcagatcgct
    12721 actcacgaaa agacatggat cagttaggtc aatatgtagg acaattcgga gctaaggggc
    12781 ttgcatggct gaaagtagaa gaagacggat tgaaaggtcc aattgctaaa ttcatgggag
    12841 aagcaacaga agcaatcatt aaagcaacag atgcaaaacc aggtgacttg ctgatgtttg
    12901 gcgcagataa aagtgaaatc gtcgctgctg cattaggtgc tatccggaca cgtttaggaa
    12961 aagaattagg actaattgat gaatcgaaat tcaatttctt atgggtaact gattggccac
    13021 aatttgaatt ttcagaagaa gaaggtcgat atgtctctgc tcaccatcca ttcacgatgc
    13081 caaaagaaga agatatcccg ttattggctg aagaccctgc aaaagtctat gcagaagctt
    13141 atgatatcgt cttgaacggt tatgaactag gcggaggttc tatccgtatt catactcgtg
    13201 agcttcaaga aaaagtacta gaaactctag gctttacaaa agaacaaatg gaagaacaat
    13261 ttggtttctt gttaacagct ttagattacg gattccctcc acatggcggt attgcattag
    13321 gattggaccg ttttgcaatg ttattagccg gagaagataa tattcgtgaa gtcattgcct
    13381 ttcctaaaaa cggaaaagca gcagatgtga tgagtgatgc accaagtatg gtttcgcctt
    13441 tacaattgtt cgaattgaat attgatgtaa cgaatataga agaatagccg ataaaaaggt
    13501 aaaaaggatt tgtcgaatac gcttcttttg acaaccttga tttaattgaa aaacacttta
    13561 taatgaagcc ataacatata aagcggggct tttccatgaa aaaaacattc gaaaattggg
    13621 cttatcatga ttacacgacc aagctttcgg tatctatcgt ttatgcgttg atcgcatcag
    13681 tcgcattgaa tttcttttat cagccaggac atatttattc aagtggtatt actggtctag
    13741 ctcagatttt gacgactttg tcaaaatcac tgataggatt cgaagtaccg gtatcaacaa
    13801 ctttgtattt actgaatata cctttgtttg tattggcgtg gttaaaaatc gggaagaagt
    13861 ttacaatttt cacttttatc acagtcactt tgacctccct ttgtatccat attttaccgg
    13921 aaacagtcct atctaccgat ccaatcatct gtgcgttgtt tgggggggct gtgatgggac
    13981 taggtattgg gctagcgttg agaaacggcc tatcatcggg cggtctagat atcgtgagta
    14041 tcacgattcg taaaaaaaca ggaaagtcga ttggttctat ttcgatctac ttcaatgcat
    14101 tgatcgtttt tgttgccgga attctatttg gctggcaata tatgttttac agtacgttgt
    14161 ctattttcgt cagtggaaaa gtaactgatg tcgtttatac taaacaaaag aagatgcagg
    14221 taatgatcgt aacgaaacat ccagaaaaag tagtaaatga aatccaaaac tgtatgcgta
    14281 gagggatcac gatcatcaat gaagcagaag gtgcttataa gcatgataag cagacagtat
    14341 tgatgacgat cgttacacga tacgaactgc ctctacttag agaagcaatg aaagcttctg
    14401 atccacaagc ttttgtaagt attgcagaaa acgtccagat acttggtcgt ttctatgagg
    14461 atggcattta gatcgagttt gacaaaaatt catgaaatac tgctgtagac agacgttcgt
    14521 ttgtctgcag tttttttcta ccatagta
//
LOCUS       DAJSMI010000063        14253 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJSMI010000063 DAJSMI010000000
VERSION     DAJSMI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.063"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            200..2671
                     /locus_tag="ON430_001498"
     CDS             200..2671
                     /locus_tag="ON430_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3219421.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2774..4510)
                     /locus_tag="ON430_001499"
     CDS             complement(2774..4510)
                     /locus_tag="ON430_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCR3219422.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4507..5211)
                     /locus_tag="ON430_001500"
     CDS             complement(4507..5211)
                     /locus_tag="ON430_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3219423.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5342..5845
                     /locus_tag="ON430_001501"
     CDS             5342..5845
                     /locus_tag="ON430_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCR3219424.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5805..6143
                     /locus_tag="ON430_001502"
     CDS             5805..6143
                     /locus_tag="ON430_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCR3219425.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6164..7582
                     /gene="ffh"
                     /locus_tag="ON430_001503"
     CDS             6164..7582
                     /gene="ffh"
                     /locus_tag="ON430_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR3219426.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7694..8272
                     /locus_tag="ON430_001504"
     CDS             7694..8272
                     /locus_tag="ON430_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR3219427.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8276..8797
                     /locus_tag="ON430_001505"
     CDS             8276..8797
                     /locus_tag="ON430_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3219428.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8876..9430)
                     /locus_tag="ON430_001506"
     CDS             complement(8876..9430)
                     /locus_tag="ON430_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3219429.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9683..9958
                     /gene="rpsP"
                     /locus_tag="ON430_001507"
     CDS             9683..9958
                     /gene="rpsP"
                     /locus_tag="ON430_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR3219430.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9970..10221
                     /locus_tag="ON430_001508"
     CDS             9970..10221
                     /locus_tag="ON430_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCR3219431.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10346..11137
                     /locus_tag="ON430_001509"
     CDS             10346..11137
                     /locus_tag="ON430_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219432.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11307..11828
                     /gene="rimM"
                     /locus_tag="ON430_001510"
     CDS             11307..11828
                     /gene="rimM"
                     /locus_tag="ON430_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR3219433.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11818..12579
                     /gene="trmD"
                     /locus_tag="ON430_001511"
     CDS             11818..12579
                     /gene="trmD"
                     /locus_tag="ON430_001511"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR3219434.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12715..13062
                     /gene="rplS"
                     /locus_tag="ON430_001512"
     CDS             12715..13062
                     /gene="rplS"
                     /locus_tag="ON430_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR3219435.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13320..13613
                     /locus_tag="ON430_001513"
     CDS             13320..13613
                     /locus_tag="ON430_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCR3219436.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4771 a         2573 c         2904 g         4005 t
ORIGIN      
        1 aacgaagaaa aagtgttagt agaaaatcgc cttcgatttt ccaccaacac aaaatattat
       61 agaacccttt ttttttgcta tatgacagga atgaaaggtg tttgcaagct taatgtgagg
      121 aacactttcc atatctgttc tatactggat aggagaagtt tggaaatatc cagaaaacga
      181 aatcaatcga ggtagccaca tgaagaataa atggataaat cgaagtaaaa aaaagaaagt
      241 aacacaaaga tcatcagata aagtcatccg tctttttcca tcgtataaag aaggattaac
      301 ggcagatcaa gtcaaggaac gaatagaaaa aggtgcagca aataattcag tcgatcccac
      361 attcaaaaca aatcagcaaa tcgtattgga aaacatattt acgtatttca atttgatctt
      421 tcttatctta gccattttgc tttgtttagt agaatcctat aagaacttga cttttttacc
      481 agttatcata gccaatacag gaatcgccat ttttcaggaa attcgatcca aaaagatctt
      541 ggacgaacta aatgttttaa atacagcaaa agtcaatgtc atacgagacg ggatagaaca
      601 aacaatagat atagaagacc ttgtaataga tgacattgtt attttagaga ctggatatca
      661 aattccagca gatgcagagg tcgtggaagg gaaactgcaa gttaatgagg ctttattgac
      721 aggcgaagca gatgaaataa ccaaagaaat aggtgatcca ttaatgtccg gcagtttcat
      781 tgtttccgga aaagcttacg cccgattaga caaagttgga gcagattcgt atatttccca
      841 gctgacagtc aaagccaaga caatgggaga aggagaacag tcggagatga ttgcttctct
      901 taatcaattg atcaaatgga ttgggatcat cattattcct atcggtatcc tgcttttttc
      961 tcaaagttac ttttttaatg gaaacacgat caaagaaagt gtcgttgcaa tggaagcagc
     1021 attgatcggt atgatacctg aaggactgta cttgctgacg acgattgcat tagcattaag
     1081 tgcgactaaa ctagcaaagc aaagagttct tttacacaat atgaaaagca ttgagacatt
     1141 ggcaagggta aatgtccttt gtgtagataa aacaggaacg atcacagaaa ataaaatgtc
     1201 tgttcaaaaa atgatcgtct ctaaaaaaca agaaataatc agtcagtcag atcaattgga
     1261 agcatgcatt gctgactatg caaaagcgat ggcaggagac aatgcaacaa tggaagctgt
     1321 taaagcatat tttacaaaaa ccactgatca atcctatcac catgttattc ctttttcttc
     1381 tgtccaaaaa ttcagcagtg tagcgttggc agacaaggtg tatgttttag gagcgcccga
     1441 aatggttttg cgcgatcaat tgccggaata tgcggaagag tttgtttctt tcgctgaaaa
     1501 aggctatcgc gtgttggtct ttggtgtgta cgatgggatt cttgaagaac cagtattgac
     1561 agaagcagtc acaccattag gctatattct gattgctaat cctatcagaa aagaagcaag
     1621 agccacattt gattacttca aaaaacagca agtagccatc aaagtgattt ctggagataa
     1681 tccgctcacc gtatccaatg tagctgtcca agcagggatt tcacatgctg atcagtacgt
     1741 tgatgcatct aaattatcag aagaagaata tgaagcagct gtcgaaaaat atacagtttt
     1801 tggacgcgtc aagccagaac aaaagaaaat cttcgttcaa ttgataaagc gtaacaatac
     1861 agtagctatg acaggtgatg gcgtgaatga tattctagcc atgaaagaag cagactgcag
     1921 tatagcgatg gcttccggaa atgaagccgc catgcaggcc tcacaagtgg tcttacttga
     1981 ttctgacttt tcacgcatgc cagaagttgt tgcagaagga cggcgcgttg tgaacaatat
     2041 tgagcgatca gccagcttgt ttttagtgaa gaatattttc tcctttttgc tctcactgtt
     2101 ttcagtgatc tttgctctga cctatccttt agaaccatca caaatcacat tgattagttt
     2161 gtttactatc ggacttccat ctttcttgct tgctctagaa gaaaacaaaa agcgaatcag
     2221 agggaaattc atcatgaatg tgatggaaaa agctgtgcca ggtggactaa cagatatgat
     2281 cgtagtcggt gcgttggtga tctgcggggt gactttgaat ctaaataaaa cagatgtttc
     2341 tacagcatct accatgcttt tgattgcagt agggttcctc gttctctata aaatctgttc
     2401 accgctgaat aaattcagaa gtcagatcat tcttttctgt gctagcggta actttttttc
     2461 ggtcatcttt ttgcataagc tgttttcaat caccggtatc tcagctgtat cacttttgct
     2521 gttgagcatt ctatttttct cggcagattc gatttttagg catttgacca cattggtaga
     2581 atatttgttt gaacgtaaaa atgaaaaaga gcaaaagccg tttagtcgcg tagctaaaaa
     2641 atttctatcg atatttaaac gagaaaattg acgaaaagta tctcaaaaaa gagtaaaaaa
     2701 gaacaaccac tcgtgtatct ttcgaagcag gatgagtcat ccaactttaa aacgcacgag
     2761 cagttgctct tttttacatt ttcggaatgg taacggtgaa agtcgaacct gttccaagat
     2821 gactgtctac agtaatcgta ccattaagga gttgcacgta gtcttttaca atcgctaacc
     2881 ctaatccggt cccgcctgaa tgtcggcttc gcgccttgtc tacgcggtaa aaacgctcaa
     2941 agatccgttc ctgatcctca atgtcgatac ctataccaaa gtcttggaca gaaaacgata
     3001 gatcatctgt tgcctgatag cggataataa tctcgctgtc gctttttgaa tattggacag
     3061 cattttcaat cagatttttg ataattggat aaaggatatc tgttttagta gtaaatggtt
     3121 cgttctttcc aatcaatcga atcgtcaacc gtttcttttc gatgattggc tgataatttt
     3181 gaacgatttg ctggaaataa ggttctgtat aaattgtctg gatctcgtaa gaattctcgc
     3241 catcttttga cagctggatg atttcccgaa tcagtttatc taaccggatc gcatcttttt
     3301 gcatgatctc aagaaaagaa gtcagagttt gcgggtcatc ttttgcacca tccagtaaag
     3361 tctcggtaaa accaatcagt gaagtcacag gagtttttaa ttcgtgagag acgttgccaa
     3421 cgaaatcctt ttgtagcttt tctaaacgtc gcactttcgt taagtcatac gccaccccta
     3481 gaatctgtcc ggtaccttct gaattagtaa agtagcgcaa actcaagtct aatgtctgcc
     3541 gaccttctgt aagggtgatc tcctgatgga tcagcggatg ctcaggagtc acttgatgaa
     3601 tcagctgaat gagttttggc tcttgaatga cttctgtgta ccgctgttca ggttgatagc
     3661 ttaccacgtt taaatgtagc tgcatctttg gattaagcag tagtaaacga gaatcagcat
     3721 catcaatcag gaatacgcca atcatcagtt cgttcagtaa cgtatataat tgctcttctg
     3781 tggtagtaca cgcccgatac atcttactca tttgttcgct caacgcatta atcgtctgat
     3841 acaattcttc ccattgatcg ggtgtttgca tgattacttc cgcctgatta ggattttgca
     3901 ccattttttt tagtacgggc agcacggttt tcaatggctg gtttttttga tagatcagat
     3961 aatagatcat tcctgcaaga acaaggaaga aagacagaaa gaagaaaaag acccaacggc
     4021 gaaaactctc agttctcggt aaaaaaacgc ttgtcggttc tgcgatacgt aaaatggctt
     4081 ctaactggcc actttttttg actggtaatg cgacatataa aagatcctca ttcaaagtag
     4141 tacttttgcg caaagcgcta cctaggttcc caccattcaa tacggctttg atttcgggac
     4201 gggagtttcg ctgttcattc aatgtttcat tgtttgtgtc aaataagatc tttccagtag
     4261 catccattaa agtaatccgt tcgttcgact gatggacgaa ttcttccaaa gcagttttgt
     4321 tttgcgctgc ttggaggttt tcgacatcta attgccggat cagcagttgt gctttttttg
     4381 tcaggtagct ttcttgctgt tccacgatct gctgttgaaa ataatgggaa atcaattgcc
     4441 agccaccaaa aaacaaacta actaaaagaa taagaaagaa gagacgttca taaatttttt
     4501 tccatttcat cgtttgggtt cctgaaactt atagccaaat cctctaatag tcaccaagta
     4561 ctttgggtgt tttggatctc gctcgatttt ttctcgcaaa tgactaacgt gtacatctac
     4621 gattcggctt tgtcctgcga agtcaaaatt ccaaatgcgg tctagcaatg tgtctcggtc
     4681 gatcacacgg tcttttcgtt tcatgaaata aacaagtaat tcaaattctt tcggtgttag
     4741 ttcaatcaga tggtcgtcta cggtcacttg ataattggtc aaatcagcag tgatttgtcc
     4801 aatcgataga tattcaggct cagcttcatc ttttgattgc ttacgaggtt caattcgtct
     4861 gaatattgct ttcatacggg ctaatacttc gcgaggacta aatggtttgg tcaaatagtc
     4921 gtcagcacct atttctaggc caatgatccg atccacttgg tcatctttag ccgtcagcat
     4981 caaaatcggt gtttcatttt tttcttgacg cagccgctga gtgatctcca tgccatccat
     5041 cgttggaagc atcacatcta aaatgatgaa atcaaatgat tgttctaatg ctaattctaa
     5101 tccttcgcct ccatctgtgg cactggttac cttgtagcct tctttttcca aattgaacgt
     5161 caagagcgtt acaatagaag gttcatcatc aacaactagt atttttttca ttcaaatctc
     5221 ctttaaatag gtttcattct gtttatattt tagcatagaa tcagtacaaa gggctatttt
     5281 acaaaagaaa taaatggtat aataggaagg cactttgctg aatagaaagg aagagcagtc
     5341 aatgattgga atcagtgcat gtttaggcgg tctggcctgt cgctatgatg gacagaaaaa
     5401 agaagtacca gaattgatgg aactagttga aactggagaa gctgtcatgg tctgtccaga
     5461 agtgatcggc ggattaccaa ttcctagaga tcccgctgaa atcattggtg gaaatggatt
     5521 cgatgtgtgg aaagatcaag caaaagtatg gacgacaagc ggcgaagatg taacggaagc
     5581 gtacaaaaat ggcgcgatca aagcctatca aaaattgcag gaaaaacaca ttgatctagt
     5641 catcatgaag gaaaaaagcc cttcatgcgg aacccattcg atttatgacg gaagtttttc
     5701 cggaataaaa aaaaatggtg ccggagtggc tactgcttat tttattcagc aaaaaatgac
     5761 tgtgctctct gatgaagaat ggctgaaaac tagaggtgag cagaatggaa atcgaaaaga
     5821 ccaatcgaat gaacgcgctc tttgaatttt attcgacctt attgacagaa aaacaaatga
     5881 actacatgga attatattat gcagatgatt tttcattagg tgagatcgcg gaagaatacg
     5941 aagtaagccg acaagcagta tatgacaata tcaagcggac aagcaagatc ttggaggatt
     6001 atgaaaaaaa actccatctt ttttctaatt atattgttcg agaacaggta ttggaaaata
     6061 tgactgcata tattacagat aagtacccgg aagataaaaa actacttgaa tatgtacagc
     6121 aaattcaagc aatcgaagaa taaataaaag gaatgaagaa aatatggcat ttgaaagctt
     6181 aactgaacgt ctccagcagg cgatgggaaa aataagaaaa aaaggaaaag tatcagaagc
     6241 tgacgtaaaa gaaatgatgc gggaaatccg ccttgcctta ctagaagccg atgttaactt
     6301 gcaagtagtc aaagatttta ctaagcgcgt acgcgaacgt gcggtagggg cagaagtgct
     6361 ggaaagttta tcacctgcac aacaaatcgt aaaaatcgtt gatgaagaat tgacgatcac
     6421 acttggttct gaaacagcag agttaaataa atcaccaaaa ataccaactg ttatcatgat
     6481 ggctggtctt caaggggcag gtaaaacgac ctttactggt aaattagcca attatttaaa
     6541 gaaaaatgaa aatgctcgtc cgctcctaat cgcaggggac gtatatcgac cagctgcgat
     6601 cgaccaattg aaagtattag gtcaacagct agacgttcct gtatttgata tgggaactga
     6661 agtcagcccg gtagagatcg tccgtcaagg gatggagtta gcaaaagaaa agaaaaacga
     6721 ctatgtattg atcgatacgg caggacgtct gcatatcgat gaaacattga tggatgagtt
     6781 gaaacaaatc aaagaattga cgcaaccaaa tgagattttg cttgttgtcg atgccatgac
     6841 tggtcaagat gccgtcaacg tagctgatag cttcaaccag caattaggta ttacaggggt
     6901 cgtcatcact aaacttgatg gggatactcg aggcggggca gcactatcga tccgctcagt
     6961 gacaggcgca ccaatcaaat tcatcggttc tggtgaaaaa ttgactgact tagaagtatt
     7021 ccacccagat cgtatggcta gccgtatcct tggtatgggg gatatgctga cattgattga
     7081 aaaagcgcag caagactacg acgagaaaaa agcggaagaa cttgcacaga aaatgcggga
     7141 aaattcattc gatttcaatg actttattga acagcttgat caagtgatgg gtatgggccc
     7201 actggaagat ctaatcaaaa tgatcccagg aatgaatcaa gtccctggaa tcgaaaacgt
     7261 caaggtagat ccaaaagatg ttgaacgcaa aaaagcgatg gtttattcta tgacgccagc
     7321 agaaagagca aatcctgatc tgttgaaccc aagccgtcgt cgccgaattg ctgccggttc
     7381 tggaaacagt gttgttgaag tgaatcgaat gatcaaacaa ttcaaggaat caagaaaaat
     7441 gatgcagcaa atgtccaaag gggatatgaa catcccaggt atggaccaaa tgtttggttc
     7501 aggcgtcaaa ggaaaactag gcaaaatggc gatgaatcga atgatcaaga aaaataagaa
     7561 gaaaaagaaa aaacggaaat aagatgaaaa aaacagcttc ggtacagact gaaaatgtct
     7621 actgccggag cttttctatc agcaattttt attctttact aatacaatta ctctaaacga
     7681 agaggtggca cagatgagat gtccgtggag tgaacaaaca gaaagtatgc ggcaatatca
     7741 tgatactgtc tggggtgttc ctgaatatga tgaccaaaag ctgttccgaa aattgatgct
     7801 tgatattaat caagccggat tgagctggca gacgatttta aataaaagtg aagcattcga
     7861 tgaagcatat gatcgattcg atatcaaaaa agtcgcaaat tatggagaaa caaaaatcag
     7921 tgaactaatg gaaaacaaag gaattattcg taaccgaaga aaaatcgaag cagccgtcca
     7981 aaacgccaaa gcagtcttga aaattcaaga agaaatcggc agtttttcag agtatctttg
     8041 gtcgttttct aatggaaaaa tcgttgattc ttcttatcat tcacaagaag aagtcccaac
     8101 aacaagtcct ctttcagatg aattagcaaa agatctaaag aaaagaggat ttaaatttat
     8161 cggaagcaca acaatctatg cttttttaga agctgtcggg atcatcaatg atcaccttga
     8221 cagttgtttc cgcaaagaag aggtcgtacg tatcggcaga aagggcgaat aaagcatgcg
     8281 aaaaatcata ttttatggtg ctataagttt agatggctat ttggcaacga aaacggatga
     8341 tcttcaatgg ctttttgaca ctccaacagg taagcaaact acttatgaag ctttttatga
     8401 aacaatcgat acgaccatca tgggcagaaa aacttatcag gaagcaaaaa aatatttgga
     8461 gaccgaagca atttatccag agaaaaagaa tttcgttttt tccacccacg cacaattaca
     8521 attgccagat gctgaggtcg taaacgaaga tcctgttgct tttctaaaaa atctacaaat
     8581 gacagaagga aaagatatct gggttgtggg tggcggtcga ttgatcaaac cgctattgga
     8641 acatcaaatg attgatgaat ggtatatcca gattacccct gtattattag gggacgggat
     8701 tcctttattc caaaaaggaa catatgaagc gcgttttgaa atgctagaca cgacacgatt
     8761 cggtgaattt atcgaattgc attatgtacg aaagtagaag taaataaaat aaaaaaggca
     8821 actatatgca gaaatgtcct gtacatagtt gcctttttta ttaccgttat gctttttact
     8881 ctgttaattt gttagaaaaa tctagatacg catctaaaat cgtttggaag aaacttaatg
     8941 ttccttcaac gatattacca ttttcatcaa ttagattcgc aacgtttccg atatatgctt
     9001 ccggttgctg caaggttggc acattcaaaa agacaagtga ttggcgtaga tggtggtttg
     9061 cgccaaagcc gccgatccca cctggtgatg cagtaacgac taaacctggt ttatgatccc
     9121 aaacactgtg tccataaggt cttgagccta catctaaagc atttttcaat actgctggca
     9181 cagaacggtt gtattcagga gtaacgaaaa cgacaccgtc tagtcccctc acttcctcac
     9241 gaaaacgagt ccattcagca ggagcctgtt ctggagtttc gagatcctca ttgtaaaatg
     9301 gcagatcatc gatttttaca aatacacttt cgtacccttc aggaagcaat ttcgcaaaag
     9361 cttctgctac ttttttgttg taagaatctt ttcttaaact accaataaaa aatccaattt
     9421 tttttgtcat acgatttttc ctccttcaaa ctatctataa taatcttaca aaagataagt
     9481 aactggagca aataaaaaca cttactgaca aaagctacat agaagataga ttacttattt
     9541 ttaacaaaaa agaagtctaa aatcttctat tttctaaaaa aaatcgaatt taatcaagct
     9601 gtaaagaaaa aaagctttac agggtaaaaa aaaactgtta cactacatct tgtgaataaa
     9661 acaatggagg tggaaataaa taatggcagt taaaatccgt ttaaaacgta tgggttctaa
     9721 aaagagtccg ttttaccgta tcgtcgtagc tgattcacgt tctcctcgtg atggacgttt
     9781 catcgaaact gtaggtactt acaatccttt gaaagaccct gcagaagtag ttttaaaaga
     9841 agacttagtt cttgactggt tatcaaaagg tgcacaacct tctgacactg tacgtaacat
     9901 cctttcaaaa gaaggcgtta tgcaaaaaca ccacgaagct aaattctcaa agaaataagg
     9961 tgaccggata tgaaagattt aagcgactta gtcttaacaa tcgttcgtcc gctagtcact
    10021 tatcctgatc aagtcaagtt agacgtcgta gagtcgaaag acttttacga gtacaatcta
    10081 acagtggctc ctgaagatat tggccgtatc attggaaaac aagggcgtgt tgctaaagca
    10141 atccgcacaa tcgtttacgg tgttcggatc aatgcaccga aaaaagtgcg tttgaatatc
    10201 atcgatgata aggaaaagta aataaacgtg ctgtactcga attttcgagt gcagcttttt
    10261 tgtttttttt attaattgaa gaaatgatta tttggcaaaa caagcaagaa agcgtatcct
    10321 tataggagga gggagtgaac agccaatgag aaagaaaatg ttatttattc catttgctct
    10381 attgttaagc gcttgtagtt cggttactca agaatcagac aatacacatc tatcaagcag
    10441 tatatctgaa actgttacca gtccagaaaa aagtagtacc aaaacggctt ccaccactga
    10501 acaaacaatg gctacctcaa ataatgagaa aaagactgct ttagaccagt tgaaagaaca
    10561 acagccaaat gttcccatgc ctctagatgt accagtctct tcaggctatc tcaatatcgc
    10621 agcgacacat acaaagcaag gttattctat tttatattac agaacagatc gtcccctcgg
    10681 gttgaatgct gacgaattga atcaagaaac gcctattgca acctatctat atcaatatgg
    10741 gtttgcttct agtcaagaaa caatccaagt tttgcagcca tttgagatcg acacaaatgg
    10801 tcagcaagtg gacttaggtt ctcgaatcac agggtatcag caaggtgcag caggttcaag
    10861 ttttcttgaa tggcaagaag gaaactggcg tattcgaatc cgtgggaata atatagaagg
    10921 ccaagatccc ttactattag ctaaagaaat cgtagcttat ttggaagaaa acagtctacc
    10981 agcacctgaa caatttggta aaattactgt tgatatgggt gatacgacga atcgagccgt
    11041 agaagtaagc tggcaagaac cgaaaaatgc ctatacgatc actcatcaag atcctatgtc
    11101 tgcgttgaag atggcagttt ctatgaagag gttgtgaaat cagcgcgctg atctgagccc
    11161 atcatttaac tccaagtcac aggaagtaat caaaaatagc acgccgcatt tttgcataat
    11221 tttggcttaa taccttaaaa gaaaactaga attgcctctt cacacatgct aaaataaaac
    11281 agacaaaaaa gaaatgggga tataagttga ctgaatactt gaatgtagga aaaatcgtaa
    11341 acacacaagg tatcaaaggc gaagtccgtg ttatctcaac aacggatttc ccagaagaac
    11401 gttacaaaaa aggaacggtg ctaacactat tccaagacgg aaaagcacct gtggaattaa
    11461 cagtcaaaag tcatcgcaaa cataaaaatt ttgacttatt gagttttgaa ggacatcctt
    11521 ctatcaatga tgtggaaaaa tatcgggatg gcatattaaa agtgtcaaaa gacaactctg
    11581 tagatctagc tgaaaatgaa ttctattacc atgagatcat cggtctgcat gtattagaag
    11641 atggaactga gttaggtaaa gtaaaagaaa tcttgtcacc aggtgccaat gatgtttggg
    11701 tcgttcaacg tccgaaaaaa gctgatatat tgcttccata tatcgcttct gtcgtcaaag
    11761 aagtcgattt agaagctgga gtggtccgtg tagaaattcc agaaggactg attgacgatg
    11821 aagattgatg tattgacttt atttccaaga atgtttgaag gtcctatggg ggaatccatc
    11881 atcggtaagg ctgtagataa aggattatta gatatcaata tctctaactt ccgcgattat
    11941 tcagataaca aacatcagac agtagatgat tatccatacg gcggtggagc aggtatgctg
    12001 ctcaaagtcc agcccatcta tgataacatc caagccattg aaaaagctgc accggatgtc
    12061 aaaaaaagag taattcttct tgacccagct ggtaaacgat tcgatcagaa aatggcagaa
    12121 gaattttcac aagaagaaca cttagtattc atttgtggtc attatgaggg atatgatgaa
    12181 cgtatccgca cattagtgac agatgaagtt tccttaggag attatgtcct tactggggga
    12241 gaattaggtg cgatggtcat gatcgatgca acagttcggc ttttgccaga tgtgttaggg
    12301 aatcagactt cagctcaaac ggattcttat tcaaccggtt tattggaaca tccccaatat
    12361 actcgcccgg cagaatataa agggatgaaa gtaccagaag tattgacaaa tggaaatcat
    12421 aaattgattg aagaatggca attaaaagaa tcactaagaa ggacctatca acgtcggcct
    12481 gatcttttgg aaacacttga atggacacct caaatgacga aatttttaga agaaatcaag
    12541 aaagaagaac aagaaaaaac agcagatgaa gccaagtgac tttttctaaa atcttcttta
    12601 cagtaggttg ataatatgtt agtattttat ggtgagtgca aaagcactta actattacga
    12661 tattccgctg tgagaaaagc tcataagaat atttggaata aggagaagaa aaccatgaat
    12721 ccattaatcg aagaattaac aaaagaacaa ttacgttctg acatcccagc gttccgccct
    12781 ggtgacactg tgcgtgttca cgcaaaagtt gttgaaggta ctcgtgaacg tatccagtta
    12841 tttgaaggtg ttgttatcaa acgccgcggt gctggaatca gcgaaactta tacagtacgt
    12901 aaagtttcta acggagtggg cgttgaacgt acattcccat tgcatacacc acgtgttgca
    12961 aaaatcgaag tagttcgcta cggtaaagta cgtcgtgcaa aattgtacta cctacgtgca
    13021 ttacacggaa aagctgctcg tatcaaagaa atccgtcgtt aatcattgtt atcagactga
    13081 gaaagaaccc ttggtattca agggttcttt tttcttagca tgtaatttga acaccctgat
    13141 ccagtttttt ctttaattat tggtagggga gaataatgga ttagaataaa tctacctata
    13201 agtagtagta aaaaaaagag cactatgata atatctttct taagagtaac cgctttaata
    13261 aaaaatccag aatattctaa cataaatgga gtattcttag aatcaaaagg agaaagaaga
    13321 tggtttatac ttatacggag caagaaaaga tgaaagtttt agacacattc atgaatgatg
    13381 gaaaagtaaa acaagtacca agttcaacaa aaaagaaata catcctattg aaagaagtct
    13441 tgaaacgctt tgaccatgga gtgacttata cagaaacaga ggttaattcg atactgctga
    13501 acgtattttc tagtggcgat tatgtcgaac aaagacgtta tctaatcaca tttggctttt
    13561 tcaaaagaag ttcagacggt cgggcatatc agatgatggg aattgaaaat tagaagtttt
    13621 aatttatact cattgaaaaa agttcaagta tgcgatattg gttctacacc gcatacttga
    13681 actttttttg tcatctgtat ctataataca aagccaagaa cgttattcaa aatatggata
    13741 gctatgataa aagctaatga gcgattcgca gttaaataag caatcgtcag taaaatcgtt
    13801 ccaggagcgt acatgaaaaa atcaattgga tgcgttacat gaataaagat aattctcttt
    13861 tatttgtacg aataaatacc aaaacgtacc tcctaaaatt agattttgga cagatgccct
    13921 ttaaaaagat aacaagattt cgttcttgaa acaatatata tgcttttatt ccaatgaggt
    13981 tgtgaaaaca gtattttgtc ctgagcgtta gaaaattgaa aatggggaat agcaactcgt
    14041 actttaccat tttttaagtg aatacaagat cagctaattg aacaccgttt attcgtgatg
    14101 agggacacaa cactgcagtc gacacatttc tcttttatgt tcaaagataa caaatattta
    14161 aaacaaagaa aaatagtaga taatcccgtc ctaactcttt tattcttccc tcatagcttc
    14221 caaatttaag caatacttca attaatcaag ttt
//
LOCUS       DAJSMI010000064        13978 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJSMI010000064 DAJSMI010000000
VERSION     DAJSMI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.064"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            25..258
                     /locus_tag="ON430_001514"
     CDS             25..258
                     /locus_tag="ON430_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCR3219437.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(357..788)
                     /locus_tag="ON430_001515"
     CDS             complement(357..788)
                     /locus_tag="ON430_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCR3219438.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            867..1280
                     /locus_tag="ON430_001516"
     CDS             867..1280
                     /locus_tag="ON430_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCR3219439.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(1318..2466)
                     /locus_tag="ON430_001517"
     CDS             complement(1318..2466)
                     /locus_tag="ON430_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3219440.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(2588..3184)
                     /locus_tag="ON430_001518"
     CDS             complement(2588..3184)
                     /locus_tag="ON430_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3219441.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(3384..6026)
                     /gene="alaS"
                     /locus_tag="ON430_001519"
     CDS             complement(3384..6026)
                     /gene="alaS"
                     /locus_tag="ON430_001519"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR3219442.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            6415..6846
                     /locus_tag="ON430_001520"
     CDS             6415..6846
                     /locus_tag="ON430_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3219443.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(6891..8240)
                     /locus_tag="ON430_001521"
     CDS             complement(6891..8240)
                     /locus_tag="ON430_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3219444.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(8310..9473)
                     /locus_tag="ON430_001522"
     CDS             complement(8310..9473)
                     /locus_tag="ON430_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR3219445.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            9602..9925
                     /locus_tag="ON430_001523"
     CDS             9602..9925
                     /locus_tag="ON430_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219446.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(10108..11457)
                     /locus_tag="ON430_001524"
     CDS             complement(10108..11457)
                     /locus_tag="ON430_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3219447.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(11526..12479)
                     /locus_tag="ON430_001525"
     CDS             complement(11526..12479)
                     /locus_tag="ON430_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR3219448.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(12581..13771)
                     /locus_tag="ON430_001526"
     CDS             complement(12581..13771)
                     /locus_tag="ON430_001526"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR3219449.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         4091 a         2907 c         2429 g         4551 t
ORIGIN      
        1 gactttagaa aggtcgtgag tgctttgacc acaggaattg taaaatggtt tgacaacaaa
       61 aaaggatacg gctttatcag ttatgatgac acagaagaaa tttttgtaca ttttactgcg
      121 attgaagaag agggattcaa aactttggaa gaaaatcaag tggtcgaatt tgagatcatc
      181 gaagggaatc gaggaacaca agctgcccat gtcaagaaag gcaacgttca gactaccgat
      241 actagtatgc atgaatagat aaacaaatag agtgagacgc taaacgggat gaaccgtacg
      301 tctcactctt ttcttttcct gtaaaaattc gtttttttag gttctgattt ttaaaattag
      361 ctttttcttt ttttcgtata aggataagga attgattctg atttgggtcc gtttttttca
      421 aaatgatcaa aaaggactac ttgatctttt tttcgcatca tgaaaacaga tggggctttc
      481 tcatcttgca acatcatttg caccccagta ttagttggcg tcaaagatcg aatctgtccg
      541 acagatattt ctcggtcttt ccaaaaagca gcataacgta taatcacaga gtttgatttt
      601 aaaataaaag aacgccgcaa acctagaaat agaaaaacaa gaaaaatacc taaaacaaaa
      661 ttgcttttcc aataaggacc tgtgttttct aaagaaagaa tcagactaaa aaacagaatg
      721 atcaaggtca atgaccaata gatgatcgta tgtgccagtt caggctgcca tcgaatattt
      781 tttttcattc tctaacctct caccgattca gtaatgatat aatcttacca taacattagt
      841 ttgaacaaaa aggagaatct ctttttatgt taaaagtata cattgatgcc tctacaaaag
      901 gcaaccctgg tcctagtgga ggtggaatct tgattgtcta tcaaggtaaa caagaacagc
      961 tatctatccc gctttctata gggacaaacc atcaagcaga gttcgaagtc tttttaaaaa
     1021 ctttagaatt tctaaagaaa aacgacttac agaatgaaac gattttctgt ttttcagaca
     1081 gcaaaacact agtttctacg gtcgacaaaa acgcaacgaa gaacgagctc ttccttccct
     1141 accttcaaag aatccaagaa cttctatccg aattttcttt attgatcctg caatggttgc
     1201 ccgaaaacaa aaataaagga gcagataatc ttgcacgtca ggctctccat aaacaattaa
     1261 aagaaaaaaa gactctttaa attaaaaact ttaaggagtc tttttatagt ttctcattta
     1321 atataaaaga taattgtgat caaacgtaat ttccaattgg taatctccga ttttttcttc
     1381 tatttgacta cgtaagattc tttctagttc tgtatcaaca aactggaatt tctcagggac
     1441 cactacatca aattgcagcg tttttccttg atgaattact ctaaaatcat gaatattaag
     1501 ccctgtacct gtttcaagaa gaatttgctt taactgccgc aagatattat agtatttttc
     1561 gttatcaaca ggcattggat ccaagtgaca gacaagttcc acatccaaag actttttaaa
     1621 gtctttttct atcagatcaa taatcttatg agccacattc aaattcattg attcatctac
     1681 ttcgatgtgg actgatgcaa agcgtttatt gggtccatag ttatgcacca ataaatcatg
     1741 atagccaagt atatcatcgc aagcatttaa atgctcttcc atttcttcaa tctctgctgg
     1801 cgttgggcga cttcctaata gttcgtatac aaaatttcga agcatcgtca atccactata
     1861 aaggatatat aatgctaaag caaaacctat atatccatca atacgccatc cagttaccca
     1921 ttcgattgct gctgaaagca atacagccag tgtagtaaag acatcattat aactatctgt
     1981 tgctgtagct ctcaatgttt cagaatcaat ggattgcgca ataacctgat acattttgcc
     2041 ttgtgcaaat ttcaaaaaga tggaaatgat taggacaaaa aagacgatgg gtgttaaaga
     2101 gacattttct ggatggatga ttttttcagc agaagactct aaaaattgaa atcctacata
     2161 agtaatgatg atcgaaacaa ataacccact gatatattcg aagcgttcgt gtccataagg
     2221 atgttcctga tctgctggct ttgcagcaat tttaaatccg atcaaagtca atatggatga
     2281 agcagtatct gatagactgt tcactgcatc agccataatg gagacacttc ctgaaaaaag
     2341 cccgatcatt agcttagaga caaacaagat cagattcgta accaatccta agatacccgc
     2401 tagtactcct ttttttgtcc gctgttgcgg tatttgtttc tttttttctt cttgtttctt
     2461 agccaagttt tttacccctt ctagtttgct tgttgctcag gtaaaaagat ggagaggacg
     2521 attgttttcc tctcctctct tcctttttct atctatcgtg cgtcctatta ctattaaaag
     2581 atggtcatta ttctggtttc aacggtgttg ctgcaaataa cgcttttgct agtaatggtg
     2641 ttatgattcc cgcacctatt gcttctggta tcccattgat tgctgcaacc cctgctaagg
     2701 ttttaaataa aagagcagag tctacaccat aggcatctgc cgttgcacct gtataaaata
     2761 atcccatgaa tcctaacact aagactgtat tgaccaatga acccagtacc cctgcaaagg
     2821 ctgagctgat cgttattgat ttattttttt gataaatcca gtggaaaacc aagcctgcta
     2881 ctaagccaac aagtactctg ggaactaccg agataatcgg attcgtgaac actaatgtat
     2941 caaatggcgt tgttggcatg gcataggcac gaacaattgt agcgattccc caaaacaagc
     3001 caatcaccat gccatctttt gggcctaata ctactgcagc aataattact gtgatatgga
     3061 taattgttaa actaatgaat cctaatggaa taaaccccag ccaaggaacc atcgcttgta
     3121 cgatgatgat tgctaacaag atcgcacgta agactagtcg aaaggtctta tttctactat
     3181 tcattttttc accccttttt atttatacta atcgtaaaaa tttctgtcac tttcgtcaac
     3241 taaagaccat ccgaatccga ccatttgatt agatgcgcca tccaattgaa aaaagtatga
     3301 gggcaagact tgtttgtctt cgcccccata atcatgtttc gttcaatgtt tgaacttttg
     3361 tttttctgtc aaacgtaaac gccctattgt tgcgcaaccc attctttcgc agctttaagt
     3421 gcatctggaa taccagcagg atttttcccg ccagcttgtg ccatgtctgg acgtccacca
     3481 ccgccaccgc ctactttcgg tgcgattgct ttaatcaagt cgccagcttt tagtccagct
     3541 tcatttgatt ctttcgtcat tgccgctagc agactgactt ttccttcttg ttcgtttgct
     3601 aaaacaagaa tatctgaaat atttttttgt ttccattggt cagctaattg acgcagctga
     3661 ttcatatctt tcacttttac ttgtgctgca atgattgttt ttccattgat cgtttctgga
     3721 tctttgaata catcaccagc ttgttgattc gctaacttgc tagctaattg ttcattttcc
     3781 ttttgtagct ctcttacttg ctgttgcaat tgttccgctt ttgaaacaac ttctttcaat
     3841 tgaggtgatt taacaattgt tgctacttgt tttagttgtt tttcttcttc ttcaagtagt
     3901 tcgtacgctt ctttacttgt tacagcctcg attcgtcgca cgcctgcacc aattcctgat
     3961 tcagaaacga ttttgaagat tccgatgttt tctgtatttg ctacatgtgt gcctccgcac
     4021 agttcgattg accagccgcc aatatttact actcgaacgt catgtccgta tttttcacca
     4081 aacaaagcca ttgcacccat gtttttggct gtatcgatat ctgtttcaat tgtttcaact
     4141 gggatagctt cccagatttt ttcgttgacg attctttcca tttcagctaa ttcatctgaa
     4201 gtgatttgac caaagtgggt aaagtcgaat cgtaaatgtc caggagcgac taaagaacca
     4261 gcttgatttg catgctcgcc aagtacttct ttcaatgcct tgtgcagtag atgggttgct
     4321 gtatggtttt tgatgatccg attgcgcatc gattgatcga tcaatagatg ataggtttgt
     4381 ccttcactca ttggtgcttt tacttctacg gtgtgcataa attgaccatt aggcgctttt
     4441 tgaacatctg taacaactgc tacgatttcg cctacttcat tcatgatcca tccttgatcc
     4501 gctacttgcc cacccatttc tgcatagaaa ggtgtttgat caaagatcat ttgagcttca
     4561 ccttctgcga cttcattcaa aagagactca cctttaacga tgaccaataa ttgactatct
     4621 gattcttgtt ctgtgtatcc aatgaattta ctttctactt tgatatctgt caataaagca
     4681 gattgtaccc ccattgattt ttcagtactg cgggcagagc gagcacgttc gcgttgtgca
     4741 tccatttctt tttcaaaacc agcatgatca actttcagtc cttcatcttc tgccacctct
     4801 tccgttaatt ctaccgggaa gccatatgtg tcgtatagtt tgaagatgtc ttttcctgct
     4861 agagtcgtcc cgttttcgtt ttttacttct tggatcactt gatttaagat ttccaaacct
     4921 tcattgattg tttcattgaa acgttcttct tctgtacgga taactttttc aataaattct
     4981 ttttgttgaa gtacttcagg ataataactt accatgattt ccccaacaac agggactaaa
     5041 cgatataaga atgcttgttc aatacctaat tttttcccat gcatgacagc acgacgaagc
     5101 aaacggcgta agacataccc tctcccttca ttcgatggca atgcaccgtc gccaatagca
     5161 aatgaaaggg cgcgaatatg atctgcgatt actttgtaag aaatatctgt tactgggttt
     5221 tcaccatatt ttacttgttt gcttagcttt tctacggcgt ggataatagg cataaacaag
     5281 tctgtctcaa agttcgttga cgcatcttga acgattgaga ccatgcgttc caatcccatc
     5341 cccgtatcaa tgtttttatg aggaagtggt tcaaatgtgt tatcaggctt atggttgaat
     5401 tctgaaaata ctaagttcca gatttctaaa taacgttcat tttctcctcc tggatagttt
     5461 tcaggatcgt cttctgctaa atcgttataa gcttctccac ggtcatagaa aatttccgta
     5521 tccggaccac atgggcctgc accgatatcc cagaagttgt cttctacatc aataatgtga
     5581 tctagtggca ggccaacttc ttcatgccag atgcgttttg cttctgtatc ttttggataa
     5641 actgttacat aaagtttttc tggatcgaat gccatccatt ttggatcagt caaaaattcc
     5701 catgcccagt ggatggcttc atttttgaaa tagtcaccaa tcgaaaaatt acccagcatc
     5761 tcaaacatcg tatggtgccg tgctgtttta ccgacatttt cgatatcatt tgttcgaata
     5821 gatttttggg catttgtgat tcttggattt tctggcacaa ctgaaccatc aaaatatttt
     5881 ttcaatgttg caacaccaga gttgatccac aataaggttg gatcgttcac ggggatcaaa
     5941 gacgcactag gttcaatcgt atgtccttta gattggaaga aatcaagaaa catttgtcga
     6001 acttgtgcac tacttaattg tttcatgtca ttacctgctt tctataaatt aaatacaaaa
     6061 aaacaccatc acagccaagg acgaaaactc gcggtaccac cttgattgca atgatgtgta
     6121 atcattacct cttaaactcg ataacgctga gctgcgtcgg tatttctacc aaaatagaaa
     6181 aagggagcaa tttgattagt gactattctt ttttcagcca agaaagaatt ttctgtgagt
     6241 cttaaaaaaa caaatcatat ccttttcgta cttaattagt atacgcattc tattgatcac
     6301 tgtcaatatc tcaattttta ttttcatttc cttttggtca tactccacga tttttggtac
     6361 gataagaata aaggaatcaa tacatctctg ctattcatga caaggaggaa tatgatgaaa
     6421 aaaatagttg gagcggatga atggatacgc gcagctagct gctttttgag atatcaagtt
     6481 tttgtattag aacaagggat tcctgcagaa ctagaattcg acggaaaaga tatcccgcaa
     6541 caaacctatt atctatttct tgatcaaaaa agaccagtag ctactttgcg ttatgaaaag
     6601 aaaacaccta cttgcttcaa gccggaacga ttttgcgtag caaaagaata ccgaggaaaa
     6661 gggttaggcg gtcagttgct tgaagctgct gaaaaacggg cggtttcaga aggttgtcag
     6721 actagttatg tagtagctga aatgacggct gtagattttt atgaaaaaca agggtatcat
     6781 acttgcacag aaccttttat tgaagatgaa gtactttgta tcggaatgaa aaaaagtcta
     6841 gcttaaaaac aagagattgt gacacgcctt ttgtcacaac ctcttgtttt ttatttatgt
     6901 gaatttttct tatttttacg agcagtacgg ctttgctgtc tgcgttcgat tttgcgttct
     6961 tttttatttt tttctgcaat tgcccaatcg atttttttct tataaccagg cttgatcttt
     7021 ttctttttct tcttcacaag accaatcagc gtgggatcta atacatcttt tgatttttct
     7081 cgtttggcac gtctattacg atcgtatgtt ttgacgattt ccccgttttt gatttctttt
     7141 ggttcaaatg agacgcccaa tttttctatc gcatcaatgg cttgctcatc atttggcgaa
     7201 tataacgtaa tggccgttcc ttttaagcca ttacgtccag tacgtccgac acgatggata
     7261 aagaagtcta gatcttctgg aatttctgca ttaataacat gagagacacc ttcgatatcg
     7321 atccctctag cagctaaatc agtcgctaca acatattgat actccagatt ctgtacttgt
     7381 cgcatcactc gttttctctc tcttggggtg atgtctccat gaatctttgc tactttcaag
     7441 ccttggtttt tcaagtaatc ggcaatttca tccacatgct gttttgtatt ggcaaatacg
     7501 attgctaaat aaggatgacc aattgtcagc agttgataaa tcatttgatt ctcatcttta
     7561 ccttttgttg aaatcagcca attttcaatg tcttcagaaa taactgcttt tggtttgatt
     7621 tcttcaatga ttggattttc catgtatttt cgtaagaatg gacgaagttt ttctggaata
     7681 gtggcagaaa aaactaaaaa ctgcagcttt tctggtaatc gacttgcaat ttggtctact
     7741 tcatttaaaa aacccatatc caatgtcata tccgcttcat cgacaacaaa tgcaaatgcg
     7801 gtgtgaatct tcagcgcttg ttcattcatc atgtctaata ttctaccagg cgtgcctact
     7861 acaacctgtg gctgttggtg atgcagacga tttaattgac gctgtttgtc tgtaccgccg
     7921 acaaaattcg agacacggat ctccggtgta gaaaatttag cgatttgtgt agctgcttca
     7981 tagatctgtg cagctagttc cctacttgga gctgtaataa caacttgtac ttcctctttt
     8041 tctgcattga ttttttcaaa caaaggcaac aaaaacgtat gtgtttttcc tgtaccagtt
     8101 tgagattgcc caatgatatt ttttcctttt gcaattaaag gaatcaaacg ttcctggacc
     8161 tctgtgggct gtttgaagcc ctttgcggtt aacgcttcat tgataaacgg tttgaagcgg
     8221 aatatatcaa aattattcat tcgtgcacct cttctcttta aattatcacg actttggcat
     8281 tataacacag acaaagcgtt tttgctcagc taatttcgag aatcatcatc ctttgtttgg
     8341 ccaatcgtgc taacagtata tgtgtgacta gctttttcat aaaacagaat ccgttttttt
     8401 ctcaaaaatg atagcaagat atttaaaata aaaatacctt gtagtaacaa gacaccgaga
     8461 gaaatgaata ctgcactcat ctgcaacacc gtattcgttg tcgaattcat gatttgctga
     8521 agcaactgcc ccaaccagct taaaccataa aaaagaaagt acagataccc gtagcggatc
     8581 atcaatgctt ttaaagaaat tcgttcgcgg tcttcttcca ccaaacggat acgcacaatt
     8641 tttttgccaa gtgtccatcc attggttata aacggtacaa tcataaagta tacccctact
     8701 tctaataaaa gccaccagat atgagaagta actgcccctg aatcgaacgc aattttaaag
     8761 aaaatcgccg ctaccaaatt aataagagat aaaaatgtcc agtcgatcaa ataagcgacc
     8821 aagcgtcgag tcaatgagac agtctttcct ttttcataag aaagttcatc cattcgtctt
     8881 cttgtaggaa gcataaatgt aaataacggc gtaacgacaa aaccaatgat tccacccaat
     8941 gtatttgtca ctaaatcatt cacatcaaat aagcggtatg gtcttgggta gataaaataa
     9001 agtccactta gctgcgtcaa ttcaaaaaat agagaaaggc agaaactagc aatcatcgtt
     9061 ttcataaaag aaagccggaa ataatatctt aaataaatgc caaatggtat gagtaataaa
     9121 acattaaaag ctggttccaa aaatacactc tgcttcattg ccggcaggta ggtactaggt
     9181 tcagacaaga ccaataccgt gttgtctctg aaatgttgcc atgaagcaaa taaatgcagc
     9241 tccatctttg gccctgtcat ttgggcaacc gtctcctttg ccggaagtgg taagatcacc
     9301 aaaaaatacg cacacaataa ataaaaaacg aaagaataca aaataactgc ccttgttact
     9361 agaaatgttc catactttcg atattcataa ataaaaaaaa cagatgagat cgctaaggct
     9421 aaaaatggaa aaacaatcag cgcaaaacga atcggttcga tataacttgc catatgactc
     9481 ctccttgatt ctttgtatct ctcttatctt agcagtcctt tgctgtttca agcaagtaac
     9541 gaggctatta tttctcattt ctttcaaagt aaagtaagat aaacacaaag ggggtgtcga
     9601 gatgctgaat atgtactttg tttttggcgt acctattttt ttactgttcc tttatgctac
     9661 gatcgcttat gtaagaaaaa gaactacaat tcattattta ggatttattc tcctgattat
     9721 ttcgggtttc atgctagtct tcaatcttca gacttggcag caagcacttc ttgaaatgga
     9781 taaaatgact ccacatgctt tgtctaaagt gcttggctac cctgtctatc tgatttggct
     9841 tcccattttc atttctggtt gcttggtttt attaaatatt tatcgcggcg tacgccgaat
     9901 agttcagtta cgcaaatcaa agtaaaagac agaaatcggt tgagcccgat ttctgtcttt
     9961 tactttgcta tttcatcgta gacaaaacga catttacgtg atcaaaatta acgagtactt
    10021 ttgacatttc ttccacgtac tcctcattta aataaaatat tttgtttggc agagaagtgc
    10081 agttctaata aaattcgaat gttttagcta tctacgtttt aaatagagat aaatcgggat
    10141 accagcaagt gtcaatacta tccccatcga agctaaaaag gtctgagtga taagcgtgct
    10201 tgatacgata aaagtccctc caatgattgc tactagtgga ataaacggat acattggaac
    10261 tttgtatggt cgaaacaaat caggttcttt tttacgaaga agaatgacac caacaaagac
    10321 catcgtataa aaaatccaaa tgacaaagat cagcatatcc gttagtgtat caaaaccacc
    10381 gatcatcatc atgccaatag cgatgactag ctctaagata cctgcggcta caggaacttt
    10441 agttttatca tgaagtttag caaagagctt gctaaaaggc aggttgtttt cttttgccat
    10501 aacataaggg agacgcattc cagtaagggt atagccattg atggttccat aaacagaaat
    10561 caagatacca actgtgacca gtcttccacc gaagccgcca aaaatcattt ttgcaacgtc
    10621 acttgccgca ttgctgtttc ctgcgactcc gtcgatagat gcggttctta agaacaccgc
    10681 atttactaat aaataaacga tcatgattcc gatgattcct aaagaaattg cttttggcaa
    10741 atctttcgcc ggctttttca attctcccga aatatttcct acgtgtatcc aaccgtcgta
    10801 tgcgaacatc gttgctagca agcccgctcc caaggctgag aaaaatgaaa gattttctcc
    10861 tgcttggatc ggaaacagtt ggaagtctac tccttcttgt cggaacaaac cgaaaatgac
    10921 gatcacaaac aaaggaataa gcttgcagac aagcgtaatc gactgaaaag cccctccagc
    10981 tttagaacct aaaaaattga tcaacaggat cgatacagca gctgtaacag ctacgggaac
    11041 aatcattgac tgggaaagac caaatagatt gacgaactgt gtaccgaaaa tgatcgacaa
    11101 ggctgctaca tttgctggaa aataaatcac gacttgcgcc cagcccaata aaaaagctgc
    11161 tgtatttccg taaatccgtt cgatatattt aatcatccct cctgtttctg gaatagcagc
    11221 tgccagttca gctcctgtta gtcctgcaca gacagaaatc ataccaccga gaaaccaaga
    11281 aaacatatgc aaactggcgg atcctgtaac ttctgctacg cttgcggctt taaaaaatac
    11341 ccctgcaccg atcactgtgc ccataacagt cgataaagca gtaaaaaatc ccatcgtacg
    11401 ttttaattca ttttctattt ttacttcttt tactggcgtg gcttgatatt cttccatccc
    11461 ttgtcccctt tcataccata gccaatccca aaagtataaa aaactttttg gatgtggttc
    11521 ttgttttata ccgtctctac caatttttct ttcagttttt ccaacatatc ctttgtcatc
    11581 acatctaagt cgtatttcgg cgaaaagccc cattcctctt gcgcacaaga aacatctagt
    11641 gaatttggcc aagattctgc tattgactga cgtacaggat ccacatcata atccatttca
    11701 aaatctggta tgattttttg aatagatgct ttgatctctt ccgggtcaaa agacatcgcc
    11761 gatatattga aggcatttcg atgaaccaat cgatcaggat ctgcattcat gagttttata
    11821 atggcatcga tcgcatctgg catatacatc atatccattg atgtaccttg atcaatgaaa
    11881 ctagtgtact ttccttcttt gattgctgaa taataaatat cgactgcgta gtctgtcgta
    11941 ccgcctcctg gcaaggttgc ataagaaatc aatccaggga aacggacacc acgagtatct
    12001 acgccatatt tcacataata ataatcacat agtagttctc cagctacctt cgttacccca
    12061 tacatggttg taggacgctg aagagtatct tgtggggtat gatcttttgg agtatttgga
    12121 ccaaacgcgc cgattgaact tggcgtaaaa aatttcagct ggaattctct agcgacttcc
    12181 aatgcattga ccagcccagt cacgttcaaa ttccaggcta attgtggttt tgcttctgca
    12241 acagctgaca aaagagcagc caaatgaatc aatgtgtcca cttgatattg ttctgctatg
    12301 ctgcgcattt tttcgtaatc cattacatct aggatctcaa agatatcgtt tttctcaatg
    12361 acttcaggca tgcgtatatc tgtcgcaatg acattttcac tgcctagttc ttgacgtagc
    12421 cgtgtgatca actctgaacc aatttgtcct aaacatcctg tcaccaatac ttttttcatg
    12481 atgatcctcc ctgatctttt ttactactat cttcagatga ttaacaatat atgcgtgaac
    12541 atgttctcta attttaaata gacgaaaaaa ctaaatttag ttaaataatt tccatttctt
    12601 ttcctatttt ttgataaact gctactgctt catcaagcat ttccctcgta tgttccgcag
    12661 tcggcatatt tctgatcctg cctgtcccaa gaggcacagt tggataaacg attggtttag
    12721 cataaatccc atgttcgatc agcttcttag agaaagtctg agtcactttt tcgtctccca
    12781 aaatgactgg agtaataggt gtttcagaca taccgatgtt atagcctact tttttcaatt
    12841 cttctttaaa ataattagca ttttcccaaa ctttttcgac aagttccgga tgttcttgca
    12901 tcaaagtaat cgaagccaat gcagctgcag ctgcacctgg agttagcgaa gtagaaaata
    12961 ggaatggccg acttctagct ttcaaccagt cgattagtgt tttcgatcca gctacataac
    13021 ccccaacaac acctatagct ttcgataacg tgcccatttg catatcgatt ttgtcgctta
    13081 atccaaaatg cttgacggtt cctgcacctt ttccagttac gcctgagccg tgcgcatcat
    13141 cgacgtaggt gatcagtcca tatttttcaa caatcggaat aatttccggc aatcgtgcaa
    13201 tgtctccatc catggagaaa acaccatctg tgatatacat gattttttta tatttcttac
    13261 tttctatcgc ttcctttgct tttttctcaa ggtctttcat atcttgatgt ttaattcgga
    13321 tgatttttgc tccggataag cggcaaccgt caataataga tgcatggttt aattcatccg
    13381 ataagatagc atcttctttt gtcattactg cagagatagc gcccatattg cagttgaatc
    13441 cagattgaaa ggcgattgct gcttctgtcc ctttgaattc tgcgattttt tcttctaatt
    13501 gctgatggat tttcagcgaa ccattgatcg ttcggactgc tcctgctcct acaccataag
    13561 tttcggttgc ttcaatacat gcttttttca attcttcgcg attggcaaat ccaagataat
    13621 tgttggatgc tagattgatc atcttttttc cgtctacgat gatacaagca ccattttcac
    13681 tttctagtat gtcgatcgta ttgtataacc ccttttcttt cagttgatcc aattctttct
    13741 ctaaatacat tgttagttct ttggaagaca ttgtctccac ctccatattc atctgtcttg
    13801 cagaacatac caaacatata tcgtgtcatt catctacaaa gcaagtatgc tatatttttg
    13861 ataacgctta cactgattat gagaattatt taaccacatt ttttatttac agtatctttt
    13921 tactttcaaa tgacttatat agtatgaaca attacctttt agataatgat ctatttta
//
LOCUS       DAJSMI010000065        13855 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJSMI010000065 DAJSMI010000000
VERSION     DAJSMI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.065"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..171)
                     /locus_tag="ON430_001527"
     CDS             complement(<1..171)
                     /locus_tag="ON430_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3219450.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFL"
     gene            complement(650..1369)
                     /locus_tag="ON430_001528"
     CDS             complement(650..1369)
                     /locus_tag="ON430_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR3219451.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1418..3064)
                     /locus_tag="ON430_001529"
     CDS             complement(1418..3064)
                     /locus_tag="ON430_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3219452.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3243..3833
                     /locus_tag="ON430_001530"
     CDS             3243..3833
                     /locus_tag="ON430_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3219453.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3925..5430
                     /locus_tag="ON430_001531"
     CDS             3925..5430
                     /locus_tag="ON430_001531"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCR3219454.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5514..6866)
                     /gene="celB"
                     /locus_tag="ON430_001532"
     CDS             complement(5514..6866)
                     /gene="celB"
                     /locus_tag="ON430_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR3219455.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6866..7384)
                     /locus_tag="ON430_001533"
     CDS             complement(6866..7384)
                     /locus_tag="ON430_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219456.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7400..7726)
                     /locus_tag="ON430_001534"
     CDS             complement(7400..7726)
                     /locus_tag="ON430_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCR3219457.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7739..9718)
                     /locus_tag="ON430_001535"
     CDS             complement(7739..9718)
                     /locus_tag="ON430_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3219458.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9739..10053)
                     /locus_tag="ON430_001536"
     CDS             complement(9739..10053)
                     /locus_tag="ON430_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCR3219459.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10221..10514
                     /locus_tag="ON430_001537"
     CDS             10221..10514
                     /locus_tag="ON430_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR3219460.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10592..11704)
                     /locus_tag="ON430_001538"
     CDS             complement(10592..11704)
                     /locus_tag="ON430_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3219461.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11902..12081
                     /locus_tag="ON430_001539"
     CDS             11902..12081
                     /locus_tag="ON430_001539"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219462.1"
                     /translation="MMITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKL
                     MVVKNKKKETIVHFE"
     gene            12091..12270
                     /locus_tag="ON430_001540"
     CDS             12091..12270
                     /locus_tag="ON430_001540"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219463.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12464..12634
                     /locus_tag="ON430_001541"
     CDS             12464..12634
                     /locus_tag="ON430_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3219464.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13058..13798)
                     /locus_tag="ON430_001542"
     CDS             complement(13058..13798)
                     /locus_tag="ON430_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3219465.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4329 a         2824 c         2372 g         4330 t
ORIGIN      
        1 caagaaccca tctgctgaaa tatgaaaatt tttgaatgct acgacattcg ccattacagg
       61 tatgtaaaag aaaaaaatca tccaaagcat acccggtaca gccatcagga caagtgcttt
      121 ataacgccat agattcataa aaaaatgatt gaaccccttt cgttttttca tctaaaaacc
      181 cctttcgatt cattcgcaac gtaattatag cgctttcaat aatgagaata aataaacaaa
      241 gtatagggaa aatgaaacaa agtataggag aggttgtgaa atcagcgttt tggcctgagt
      301 attagagaaa aaaacgggaa aatagttcct catattttgc cgttttttga gctaataccg
      361 agggtcagct gattgaacac cgtttatcag aggttgtgaa ttcagcgttt tggcctgagt
      421 attagggaga tcgtgtcctt actatctaac tctgagtcac aaggaacaat ggggactgta
      481 acaaaagtct tgtcacagtc ccttattccg aacaaacggt gttccagaag tagcttctac
      541 ggaaataagc cgaaatttca caaaaaagcc atccgaaagt cagacggaca agtctgcttt
      601 tcagatggcg ctacaactac tctgcttatt cttttagcta tccattcaat tatttttctc
      661 tcacttgttc tagctcttct ttggtcatgg gtcgatattg ccctttattc aaatctttag
      721 gcagccaaaa ttttcccata cggattcttt ttaaatataa gacttctttt cctactgctt
      781 tgaccattcg cttaacttgg tgaaactttc cttcatgcaa aatcagacgg ataattgaag
      841 tttctgtctc ttgatctact tgatcaattt ggagttttgc cggcaatgtc tgctcatcgc
      901 catcaataat cagtccttct gcaaataaac ggatatcttc ttctgtcatt ataccgctaa
      961 cctcagctag atattctttc tccacatgtt ttttaggtga tagtaactga tgggcaaaca
     1021 ccccgtcatt cgttaaaatc aagaggccct ctgtgtcttt atctaatcgg ccgactggaa
     1081 aaagatcttc ccgataatcc tggtcttcca ataaatcgat tacggtttga tcatgattat
     1141 cctgagtagc agaaatgact cctgcaggtt tatgcaaaat gtagtaaaaa tctttttgat
     1201 acgtaatgat ttctcctaaa taagtaaccg catcttcgaa ttcatttact tgcgctttat
     1261 cgcttttcac cacttgtcca ttgacttcga tcgttccttt tttgattaat cctttgactt
     1321 ctttgcggct gccaagtccc atatctgcta aaaacttatc taaacgcatg cgtttccccc
     1381 atttcaaaag agcatcaatc tagctcttct gattcactta ttttattttt aatttatgtc
     1441 gtaaacgcgc tattcctggt cctagtagtc tttctgccaa gcggattttc aaagctaaat
     1501 aaccatacac taaagcccct atacctgcta ctacaagaat caacaataga gactggaatt
     1561 tgctatcttc gtttaaaaaa gcaccaaaaa tcatctttgt gatccacgaa gccaaaagca
     1621 taatcaaggt aagaataaag atcagcaatg tcctgcgcca gacaaaactt ggtttaaagc
     1681 gggctacttc atggattctc tttaagatca aggtacaaga cacagcaaat ccgatcattg
     1741 tcgccaataa agggccgtat acttcaaata aacgaatggc tgggtattga agaaggagtt
     1801 tcactagaaa cccaataacc aaataattaa ttgccgcctt gttttcaaac attccttgca
     1861 gcatgttgga aactagcata tatagtccta ggaagagtcc tacaaaactt gcttggatca
     1921 gtacattact tcctagagaa tccggtgtat aaaataatgt atttagcgga taggcaagta
     1981 acataacacc aaatgtcgcc ggaaacataa aaaatgaaaa caactgtaaa tttccgcttg
     2041 tcaattttgc cagacctact cgatctttca acgtaacagc ttctgtaatc aacggtaacc
     2101 cagtagacgc gatcgatgtt gccaatgcaa taactaccat cgtcaactta tctggattgg
     2161 cactaaaaat cgaaaaaaga tccagtaact gtgcattgga atactcggtg gatgcagaca
     2221 tgatcttcat aaaagtgaac tgatcgacta atttgaagat cgttacccct gaacccacaa
     2281 taataaacgg aatcgcttct ttaacggtat ctaacaacaa ttccttcgtt gcaatattta
     2341 ttttgttttc gctgtagtga ataaacgccg atgtatacgc ttgatgtttc ttcaaaaaat
     2401 acaaaaggac aagaatactt gctagcatac cgataaatgc cgcgaaagtt gattgcgtga
     2461 ccgccgtcac atagtcacca tccatgactt tcatgatgat gaatgtagcc aatagcatat
     2521 aaaaaatacg tgccacctgt tctacaatct gcgacagcgc ataaggcatc atttcttgat
     2581 tgccttgaaa ataccctcgt atcacactca tacaaggaaa aaccaataca gcgatactca
     2641 aagaacgcat cgttggaatc agttcttctc cgccaccaga agcatgagct agccaaggag
     2701 aggcgatgta catgaacaaa gcaaacaaca ggcctaaccc gcccatcatc tgtaatgcac
     2761 ggataaataa tcgtcgtgaa gtgccatatt cattcaatga attatagcgt gcagtttgtt
     2821 ttgcaatcgc agcaggtatt cctgctgtag agatcagtag aaacaatgca tatatattgt
     2881 atcccatatt gaataatcca ttggcagcct tagcattttc gcccatccaa gcataccagg
     2941 gaatgatata gacagcgcct agcaatcttg aaatgatatt actggcggtc atccaagcag
     3001 agcctcgtgc cattttttct tgcggtgtta attgatctaa tgctggtttt ctttgattat
     3061 ccatcggtca gattccaact ttctacaaaa cattactatc attttaattg gtaaaacaga
     3121 aactggcaat cgattttata taacttttat aagaacaatt gtttttttga attcggtttc
     3181 gtttttttgg taaagtaaag aaatatacag ccttttatgg ctaacattta taggagatat
     3241 caatgacaca agaaataaaa aaactacccg ttatattttt aggtcttttc tttatcacag
     3301 ttggattgaa ttggttcttg ctccctcacg atatagcttc cgccggagtg ggcgccatcg
     3361 gacatttagt tgaaacggcc ttttctatcc aacgctcagt tactgtttgg agcatcaatc
     3421 ttatgatgct gcttctagcc gctgtcctat taggaaaatc agtatttttt aagactgtag
     3481 cgggaagtct gctttttcct gttgttttag agattgttcc aaaagtggaa atgatctcct
     3541 cacatttctt ttcactgatt tcaggaagtc ttttgttcag tcttggggtt tacacattgt
     3601 acacagtcgg ggcctcaaat ggcggtataa cgataccgcc gattattttc cacaaatttt
     3661 ttcggcttcc aatgccgcaa ggacttttac ttaccaatag tcttatcgtc tttttgaatt
     3721 attatgtttt tggattgttg gagacactat tcgttctcct atctattaca gtcagttcat
     3781 tgtttatgaa aatactcatc cgcttgtccc ctgtgtcaag aaaatttgaa taaagctgga
     3841 ttttgtcata gcggaaacaa acgatatatt aaaaagagtg tgctataata aaccaagaaa
     3901 ttgaataaag cgagggatca atcaatgagc ttctctttag aagacatccg tcatttacta
     3961 ttaaaagaaa atctattaaa agaatttgtt tcaacaagag gctggcatct tgatgtacca
     4021 gaaaaagctg aatttcttca gttaagttat gattcaagaa aagctgatgc ttctactttg
     4081 tttttctgta aaggaatgaa tttcaaagaa gaatatttgg attcagcaat cgaacagggt
     4141 attcaatatt atgtatcaga acaaccatac gaaaattcag cagtaggaat catcgtaacg
     4201 gatatccgta aagcaatggc gcttcttgct atggcttttt acgactatcc gcaaaataaa
     4261 ttgaaagtga tcggattcac tggaacaaaa ggtaaaacaa ctgctgcgta ctttacaaaa
     4321 gcaatcttgg atcacacaac aaataaaaaa acagcattgc tttctactat gaatacaacg
     4381 ttagatggca aaacatattt caaatcccat ttgacaacgc ctgagtcatt agatttatat
     4441 cgtatgatgt cagaagcagt tgaaaatggt atgactcatc tagtgatgga agtttcttca
     4501 caagcttaca aaacacaacg cgtatacggc ttgactttag atgtcggaat cttcttgaat
     4561 atctcgcctg accatatcag tccgatcgaa catccgacat ttgatgacta tttctattgc
     4621 aagcgacaat tgatcttgaa ttcaaaaaca gttgttttaa atcatgaaag tgactatttt
     4681 gatttactta gggaaacagc cgatctattc cagattccaa cgatcactta tggtcgttcg
     4741 gaagaaagca attatcaagt gatccgttca gataaaggta ctcacggttt tactttatct
     4801 tctcatgaag atcgcttagc tatctgcgat actgcttatg atattttgct ggctggagga
     4861 ttcaaccaag aaaatgcagc cagtgcgatc attgccgcag cactaacagg tgcctccagt
     4921 caagatgcac aagacggatt aaaagaagct cgtgtaccag gccgaatgga tcaattgatc
     4981 caatccaatg gagcgcatgt ctatgtggac tatgcacata attatctaag cttgaaaaca
     5041 ttgctgaaat ttgccaaaaa tgaacatcca gatggccgtg taattgttgt attaggcagc
     5101 ccaggaaaca aagcaatctc ccgtcgacat gattttggaa aagtgctatc agaaacagct
     5161 gacatcgcct ttttgacggc agatgaccca gcatttgaag atccgaaaaa aatcgcggaa
     5221 gaaatcaatg aagcaattac taatccagat ttgatcgttc attatgaaat ggatcgtcct
     5281 gaagcgattc gacgcgcttt agcagaaagc gctccgcaag attcagtcgt tattgcggga
     5341 aaaggcgtgg atccctatca aaaaatcaat ggtgtagatg aaccttatga aggggattat
     5401 gcaatcgtga agcggttgat tgaagaataa tggtatttat atactagtgg tttcccattt
     5461 ctaagaaaaa aagggactgt gattttttgt cacagttcct tttttcttag aaatcatttt
     5521 aacttttcac tagctaattc tgctgccttt tctctttctt catttaataa tttattatca
     5581 tagaatttga taaatgggaa ccagataatg aatgcgatga ttgcacaaat gactgctaac
     5641 acagcagctt tccaatcacc accactacca acaaatgctc cgatcccgac tggtgaaggc
     5701 cacggcatct gtgcaagcat cggtttgacg atttccagcc gaatggcaaa ataagcgagt
     5761 gtagcagaag ccatcggagc taagaaaaac ggaaccgcta aatagggatt gtagacgatt
     5821 ggcatcccga aaatgattgg ttcattgata ttgaataatc ctggtacaag tgaggctttt
     5881 cctaacactt tcagctgatc agatttcgcg aggaatgcaa taaagatgat caatcctaaa
     5941 gtcgcacctg atccaccaac ggttacataa gaattgttga attctcctgc caacggataa
     6001 tttgcgcctt gtgcgttagc tgccatatta gctaatacga tcggggtcaa gaaagaagtg
     6061 atgatattcg ctccatggat ccctacgatc cacaatgcat ggatcaagaa ataaatcacc
     6121 atgacaccaa gccatgtatt cgtcaactgc gtaacaaacc caaatgggat agcaatcatt
     6181 ttgaagatat ccgttcctag tgcaactaat aatccattga tgatcatcac cacaaacgca
     6241 atgacaaatg ttgggatcag agcagtgaaa gaacgagaaa ctcctggcgg aacaacatct
     6301 ggcattttta tgacccaatt tctcttgaca cacatacaat acaattcgac cgcaataatc
     6361 gacataataa tagctgtaaa gattcctgaa gttcctaaac gttcgacaaa agagcccatc
     6421 ctaataccat ttactacagt ttctgtctca ttgatagaag caatcaagct gattttacca
     6481 tcagtcatga tcaattcagg gatacacata aagaatgcaa aaacagatag taatgccccc
     6541 gtgagcggat taacatttaa tttttgttca tctcgttcaa ttgacgttaa ttcataagca
     6601 aacacaatgg caaagtaaat agataaaatc cccatcgtca ttgtattgac gatcatgtac
     6661 aagtcactaa ctttgaaaaa agttgcatta aaaaatcctt ctaaaaatgg gaacggtaat
     6721 ggcaaaatat ttaaaaccaa aaacatcgag cctacaatcg tgaaaggaat agaagccatc
     6781 cccgctgcca tgacagcccg aacgatttta agttgcgcaa ttttccccat aggtcccatc
     6841 aggtatttct gcaaaaaatc aaacattagt tattctcctt tcctaaatat aacgcttttt
     6901 catattgttt aatacggata tactgttggt cttggttgag tgaaattttc tttaaacgat
     6961 actgtataaa tgcactggca aaaataccgc ttacaacaag aatgaaaaca aataactgtc
     7021 ctccattgtt atttgccata aaaggataca agaatgtaaa tcctgaggta aacaacactg
     7081 gaatcactaa taaattaaag acactctgga acaaaaaata ccactttgtc catggaacgt
     7141 tcgtttggtg ttttcgataa agctttactt gttctcctac tgcaggcaaa atagctaaaa
     7201 tcagaatcat cggtaaacta cttcctaaac ttttagctga tagaagcaga atcatccatt
     7261 gcatgttcat gaaaaagaaa aaggcagtca ggtaacgtac gattagataa cgattgaaat
     7321 acatgttttt caaactaagt tgcttttttt ctaattcgat tttccttttt actgtatcct
     7381 gcacaatgat ccttcctttc tatttatttt tttgatacaa ataattcatt tcctgtgcaa
     7441 cttccaataa tgtcatcgtc gtcatcagat gatcttgtgc atggaccatg ataatttcca
     7501 tttctatttt tgtcccgctg gcatattctt tcagaagttg agtttgtgac tgatgcgcat
     7561 gaagaatctc ttcatttgct tgtcgcaact tctcttctac ttcttcaaac atctctttac
     7621 gcatcgagcg aaatgcttca tgtatcagtg tccgtgcatt tccgctatgg aaaatgatat
     7681 caaaagaagt cacttgtagt gtttcactgt ttattttttc agccatctgt tttcctcctt
     7741 attcgatcag tttgataaat ttactgctga aattctcaaa aataggttca gctaagattt
     7801 cctgctgtac ttttagctgg tcaactaacc taacgattgc tttagtcacg accgtaattc
     7861 cttcattttc gacataagaa ggcgaaacta aaaaaacaaa atgaatttct tgatattgat
     7921 cccactgcat cccagaagga atcaatgcaa cggctacctt tgtttcgatt ccaactggaa
     7981 cagcgggatg tgggactgcc actgtatcac tgaatatgat ttgtcccata ttttcacgtt
     8041 gctggatctg ctttcgcatt tctcttatat atccttcttc ttcatttatg ctcagctttt
     8101 gcagcaaatc atgaagaatt tcttcctttt tcggttcgta cgtataagtc ttgaaaaatt
     8161 tttcagacaa ctgttcggtg taaatctgct ttttttgctg tagagaaaat gctggtatcg
     8221 gtctatctga ggaatgatag ggtatagatt ctccaactac ctttcgtatt ttacgaacat
     8281 catcttcatt caaaaaaata ctgacatgta aaactggaat tttgaaaaac attgtcgaaa
     8341 gatcaataga agaaataatc aggtcgattc cttttaaagt tgtttcattg atctcataat
     8401 agccttttac acttaccact gtaatatttt taccgaattc actgactacg cgattcttta
     8461 atagttgggc actgccgtaa cccgttgcac agataatcaa tgcatgtagt ttatgcgcag
     8521 ctctttcttt ttccaatgcc gccatgagat gcaaagccaa ataggcccat tcatcttcat
     8581 tgatggtata gtttttcaaa gccggcatgt tgctgaaatg cctttttact gcgtcaaaga
     8641 ctttgggatt ttctttcttt atctcctcag tcaatgggtt ctcaatgcga atacctcggc
     8701 tcaatcgaat catcatcggt gacaaatgat cgagtagtcc attgaaaagc tggagatcct
     8761 ctactaatga aaaatgaaat tcctgagaca atcttttcag aactttcgct aattcctttg
     8821 atagttcctg atcattttga ttttctttgt gattcgactt agccatgaga tgtaatgtca
     8881 aataagcaat ttcttcttcg ggaaacgata cacctgcgat tacctcaata cgttgaacga
     8941 tccgctgcgc cacttgatac tctactcgct tagaaacttc ttgttcgatg cctaattcct
     9001 tgatttcaaa cccttctcgg agccgtttga tccccaaagc caagtgtaaa gtcagattct
     9061 gtatgataaa atccgaaact tttaacttag cttctcttgt ttcatcgaga atgataatga
     9121 ctagctcttc aaaactgatg tctgggaaaa aacggctttg ccctaaatat tctttcaatg
     9181 aatttccata atggtttcca aaaaaagtat ccatgataaa atgccgtcga tttctttctt
     9241 ccccttcgat ccatgtccct tgaccatgct tagaaacgat ttttagtgaa tatggaatca
     9301 atcgctcctt gatttcttgc atgtcctttg tcaaactgga acgactgata taaagctctt
     9361 ctgccaaatc atccataaaa agaatggcat cttccaaaag caattggttc aatatatact
     9421 tttgtcgatc ttcggactca taaaatcctt gcggttctct agcatatcca ggtactattt
     9481 cttgccgact caaaaacaag tcaaatgtca gtctttgtaa aatatgcagt tggtagccat
     9541 aacctggttt agcaatgatc ttacctccgt ttttttctac taactctttg atccgctgca
     9601 agtaattacg gattgtccga tcagataata acaattcaga agctaaacgt tgactggtga
     9661 catactcctt cttgtgatca atcaaatatc tcaacagcaa tttttccttc tgtttcatta
     9721 tcatccactc ctattccatc atatgttctc ttcgatcacc tctgctaact tctggatgcc
     9781 tgtagggatt gggacatacg cttggaaagg gatactaaca acaggctttc caacagattc
     9841 tcctaatttt ttgaatttat ccaaaaacat cgtcgtttgc gggctgatca gaaataaatc
     9901 aaaatcacta ttcttaatca tattatcgcc ttctgtagct gaaactgcat ctacgagtac
     9961 ctcttctctt ttttgcttaa agaaatccgt tgtctttgag gccatcatcg aggatgacat
    10021 tcctgccgca caaataatca atgctttttt cataaatcaa tcactccttt tatttttata
    10081 aaccaagcat atcttgtaag cgttttctat tcaaaatata tatttccgta gcattgcgga
    10141 aaaaatgtgt atcatatctt gaataataat ttctggcacc gtgatatact agtgatagaa
    10201 ttttttcagg aggggattta gtgagtatca cagtgaatat tttgtataca agtacaaatg
    10261 gacaagccag aaaatttgca gaagaaatgg tcgaaagaaa cattgtcaaa gcgattcgtg
    10321 cagaagaagg aaacgagaaa tatgactatt actttccact agaagatcca gaatctcttt
    10381 tactgatcga ccgttggaaa gatgaagaag caatcgaaaa gcatcacaaa tccgaaatga
    10441 tggctcaaat aaacgaactc cgtaaaaagt ataaattaaa aatgaaagtc gaacgtttca
    10501 ctgatttacc gtaaaaagat acacatggaa tgatagcaat ttatcatcca tgtgtatctt
    10561 cactttttcc ttatttttta ttcgatctca tctaatgatt caaaccgtgt ttttctctta
    10621 tttttcggat aagtatttca gtcaccccaa tatacatgat tcctaaacat accccaagga
    10681 tattctcaaa aatgcggatc gttactgctc ctggaacccc gaataaactg gcagtgattg
    10741 ccaaagcacc aaaagaatta aaaactgttt tatatctata ggtagaacaa acacctaaag
    10801 ctagaccacc taatataccg acccagttaa aaggaataaa ttggctgata ccaataaata
    10861 aaacacatcc aatcaatgag ccaatgattc ggtctactgc tcgttctttg aaaccaatcg
    10921 acatcaatcc atagctagat acaatagaag aaaatgcaaa ccctgcccac atcaatcgct
    10981 gaaaagggac atactctcct gcaacgatca aaagactaat gccaaatgca tagatgagca
    11041 tccaaatttt ttcctgcgaa tacatgcctt tcaaaaaaat ctctttaaac aagcttttgt
    11101 ctttgttctt ttctcgatgt tttctgtaaa ggagaactga aaaccaacaa aaaaacaaaa
    11161 ataataagct acttgtttgt gtaaagaaat tcttgttcaa cgaatcttta ggtaacgaat
    11221 aaacaataaa gagatatgaa aagccgtaca aagaagcatt ccccattttg ggatcactag
    11281 cggttgccat taatatgctg cttagtaaaa taaaatgaat caacatgccc ataactgacc
    11341 atttgatcag ctggacatac ggtgctacta atagtgagaa catgacgatt gctaaaccta
    11401 tgatcgaatg ggaaatctta taaccaaagt caacaaaacg aatactcatc aacatacaga
    11461 ataaaaccac agcgtatggt ttatgcttcg attcaaataa tgttgtaatt ataatgataa
    11521 aacaaatggc aaacaatacg atgaataatg atcgtaaaaa caatgaacaa atatacatgc
    11581 acttttcttt tttagaagct gcttctcgta ttttttgttt taaaataaac ggatctagtt
    11641 gtagagactg ataaaaatcc acctgttgtc gtcctttctt tttactttta ttttttacag
    11701 tcatctatga caggttgtaa atactatgga acatcatatg gcgttgtcat tttcttgtca
    11761 actatttttc ctattcatgt ttttataaaa aaacatatcg ttttcttgta tgattaaatc
    11821 accacaaaat aaacaactga gagaacgata tgctttatga atggttctat caaaaaaatt
    11881 ctgagatcaa tagacaaaaa tttgatgatt acagaggtct cttacgaaac cttacagaag
    11941 aaaaaaacat tgatcgttgc tgcagttctc ttccctgctc catgtgtctg tagaaattgt
    12001 ggttctgctg tggtagatgg aaataggaaa ttaatggtgg tgaaaaacaa gaagaaggaa
    12061 accattgtcc actttgaata atataatcat atgccaatgg ttatacgcct aaaaaagcag
    12121 cgctatacct gtaaaaactg tcgaagccat tggaacgctc aaagttattt tatccgtcca
    12181 aggcactcga tttcaaatca tgtcagacat aaaatcactt ctttactgac tgaaaaagtg
    12241 tcgttatttt ttatttcgaa aagttgttag gtatctttga caaccgttat tcgcacattg
    12301 aaagagttga gaagctattt accaaagtct tccaaaagaa ttctgtcttg agtgttgagg
    12361 gttgtgaatt tcgttctcac gtttccgttg aggataaact gagttttatt tccgcggatg
    12421 gcgagacaga aaaattaatc gagaaaattt acctacacaa aaaattcctc gattaacaaa
    12481 ctatttcctt agctgcacta atcctaaaga aatagaattc ttagttacag acatgaacgc
    12541 cacctacttt cagctcacca aacatgttct accaaatgcg aaattggtga ttgatcgatt
    12601 acttggtttt tccacgcctt tcaaggaggc gtaaccttta ttcacgagtt ggttgaagct
    12661 ttccgagaca aaagacccga cttgtttttc tccttattga caaagagcca ttaaaactag
    12721 agaaaaaatt acaactgatc gctgattatt tgtagaaaga aaatcaaagt attacccaat
    12781 aaacttgagt cattaggcaa aaaacactgc gaaactcaag aatgattttc gcagtgtttt
    12841 tgtatatttt ttattaacta gtattactac aaaggtttat ttttcacttc tcaacgcagc
    12901 acaaaattgt ctttacttat ttactggttg ggttcaacct tctctactcc gatactaata
    12961 tttgtttgta taccccttct aaaatcggta gtttccattc aattgatggg tactagttga
    13021 ttttaaaaac tatgcttatt caatttttta ttttagctca tatatttcgt ttctttctaa
    13081 attttatact gatgaaaaca agtagcacac ccaatattga caatataaac tggctaatga
    13141 tggtttctcc agtttttgga agatgcttat ttccactgct tgttggattg tttcctctta
    13201 tatctgagct atcagcaact tttctattct cttttgttaa aaagatcgcc acttcttgtt
    13261 ctttttcgat cttgaaagtt actggtgtcg catccaattc ataccccgtt ggcgcttgag
    13321 tttctaccag ctgatatgct cctggtgcta agccatctat cgccagtttt ccatctgctc
    13381 ctgtggttag acctgactgc agaacggttc cattggcatc ttgtaactca aaaacagctc
    13441 cttgcaggac ttctccgctt tggtcatcga tctttgtcaa cacgacaccg cctggtgtaa
    13501 gtcgattttc tttcgtcaat tctaccaccg ctgtctgact tctttcgatc tcaaactcga
    13561 ttggtgtcgc atccaattca taccccgttg gcgcttgggt ttctaccagc tgatacactc
    13621 ctggtgctaa gccgtcaatc gccagttttc catctgctcc tgtggttaga cctgactgca
    13681 gaacggttcc attggcatct tgtaactcaa aaacagctcc ttgcaggact tccccgcttt
    13741 ggtcatcgat ctttgtcaac acgacaccgc ctggtgtaag tcggtttgct ttcgtcaatt
    13801 ctaccaccgc tgtctgactt ctttcgatct caaactcgat cggtgtcgca tctaa
//
LOCUS       DAJSMI010000066        13808 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJSMI010000066 DAJSMI010000000
VERSION     DAJSMI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.066"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ON430_001543"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ON430_001543"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR3219466.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ON430_001544"
     CDS             complement(2261..2512)
                     /locus_tag="ON430_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCR3219467.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ON430_001545"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ON430_001545"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCR3219468.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ON430_001546"
     CDS             3462..3680
                     /locus_tag="ON430_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219469.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="ON430_001547"
     CDS             complement(3969..4481)
                     /locus_tag="ON430_001547"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR3219470.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ON430_001548"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ON430_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR3219471.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7377)
                     /locus_tag="ON430_001549"
     CDS             complement(6934..7377)
                     /locus_tag="ON430_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCR3219472.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="ON430_001550"
     CDS             complement(7378..8166)
                     /locus_tag="ON430_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3219473.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /locus_tag="ON430_001551"
     CDS             complement(8163..9104)
                     /locus_tag="ON430_001551"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCR3219474.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="ON430_001552"
     CDS             complement(9281..9868)
                     /locus_tag="ON430_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCR3219475.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11396)
                     /gene="obgE"
                     /locus_tag="ON430_001553"
     CDS             complement(10089..11396)
                     /gene="obgE"
                     /locus_tag="ON430_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCR3219476.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="ON430_001554"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="ON430_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR3219477.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="ON430_001555"
     CDS             complement(12785..13720)
                     /locus_tag="ON430_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3219478.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4469 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataac
//
LOCUS       DAJSMI010000067        13664 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJSMI010000067 DAJSMI010000000
VERSION     DAJSMI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.067"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            160..498
                     /locus_tag="ON430_001556"
     CDS             160..498
                     /locus_tag="ON430_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219479.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(703..942)
                     /locus_tag="ON430_001557"
     CDS             complement(703..942)
                     /locus_tag="ON430_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR3219480.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(918..1265)
                     /locus_tag="ON430_001558"
     CDS             complement(918..1265)
                     /locus_tag="ON430_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR3219481.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(1997..2578)
                     /locus_tag="ON430_001559"
     CDS             complement(1997..2578)
                     /locus_tag="ON430_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3219482.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2761..3387)
                     /locus_tag="ON430_001560"
     CDS             complement(2761..3387)
                     /locus_tag="ON430_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3219483.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3409..4740)
                     /locus_tag="ON430_001561"
     CDS             complement(3409..4740)
                     /locus_tag="ON430_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCR3219484.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4895..5296)
                     /locus_tag="ON430_001562"
     CDS             complement(4895..5296)
                     /locus_tag="ON430_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3219485.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5382..5588)
                     /locus_tag="ON430_001563"
     CDS             complement(5382..5588)
                     /locus_tag="ON430_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219486.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5683..7185)
                     /locus_tag="ON430_001564"
     CDS             complement(5683..7185)
                     /locus_tag="ON430_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCR3219487.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7203..7658)
                     /gene="argR"
                     /locus_tag="ON430_001565"
     CDS             complement(7203..7658)
                     /gene="argR"
                     /locus_tag="ON430_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR3219488.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7756..8373)
                     /locus_tag="ON430_001566"
     CDS             complement(7756..8373)
                     /locus_tag="ON430_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR3219489.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8594..9967
                     /locus_tag="ON430_001567"
     CDS             8594..9967
                     /locus_tag="ON430_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3219490.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10042..11154
                     /locus_tag="ON430_001568"
     CDS             10042..11154
                     /locus_tag="ON430_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR3219491.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQHLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11226..12864)
                     /locus_tag="ON430_001569"
                     /pseudo
     CDS             complement(11226..12864)
                     /locus_tag="ON430_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
     gene            complement(13032..13640)
                     /locus_tag="ON430_001570"
     CDS             complement(13032..13640)
                     /locus_tag="ON430_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3219492.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3684 a         2882 c         2446 g         4652 t
ORIGIN      
        1 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
       61 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
      121 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
      181 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
      241 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
      301 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
      361 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
      421 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
      481 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
      541 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
      601 tatatgcagc ttatgagcat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
      661 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
      721 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
      781 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
      841 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
      901 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
      961 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     1021 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     1081 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     1141 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
     1201 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     1261 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     1321 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     1381 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     1441 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     1501 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     1561 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     1621 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     1681 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
     1741 acctctccac taaacgatgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
     1801 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
     1861 caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac
     1921 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
     1981 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
     2041 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
     2101 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt
     2161 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
     2221 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
     2281 agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
     2341 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
     2401 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
     2461 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
     2521 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
     2581 atattctcct ttttcatttc tcatagtcct atattataca gtaaactcat cattttttct
     2641 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
     2701 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
     2761 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
     2821 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
     2881 cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc
     2941 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
     3001 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
     3061 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
     3121 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
     3181 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
     3241 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
     3301 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
     3361 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
     3421 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
     3481 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
     3541 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
     3601 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
     3661 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
     3721 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
     3781 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
     3841 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
     3901 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
     3961 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
     4021 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
     4081 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
     4141 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
     4201 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
     4261 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
     4321 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
     4381 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
     4441 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
     4501 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
     4561 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
     4621 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
     4681 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
     4741 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
     4801 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
     4861 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
     4921 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
     4981 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
     5041 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
     5101 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
     5161 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
     5221 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
     5281 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
     5341 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
     5401 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
     5461 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
     5521 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
     5581 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
     5641 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
     5701 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
     5761 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
     5821 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
     5881 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
     5941 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
     6001 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
     6061 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
     6121 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
     6181 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
     6241 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
     6301 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
     6361 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
     6421 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
     6481 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
     6541 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
     6601 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
     6661 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
     6721 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
     6781 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
     6841 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
     6901 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
     6961 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
     7021 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
     7081 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
     7141 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
     7201 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
     7261 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
     7321 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
     7381 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
     7441 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
     7501 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
     7561 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
     7621 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
     7681 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
     7741 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
     7801 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
     7861 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata
     7921 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
     7981 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
     8041 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
     8101 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
     8161 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
     8221 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
     8281 gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt tcatactata
     8341 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
     8401 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
     8461 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
     8521 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
     8581 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
     8641 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
     8701 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
     8761 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
     8821 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
     8881 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
     8941 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
     9001 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
     9061 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat
     9121 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
     9181 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
     9241 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
     9301 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
     9361 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
     9421 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
     9481 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
     9541 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
     9601 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
     9661 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
     9721 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
     9781 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
     9841 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
     9901 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
     9961 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
    10021 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
    10081 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
    10141 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
    10201 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
    10261 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
    10321 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
    10381 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
    10441 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
    10501 cttggtcagc atttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
    10561 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
    10621 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
    10681 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
    10741 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
    10801 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
    10861 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
    10921 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc
    10981 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
    11041 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg tcattggcct
    11101 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
    11161 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
    11221 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag
    11281 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc
    11341 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
    11401 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
    11461 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
    11521 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
    11581 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
    11641 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
    11701 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
    11761 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
    11821 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
    11881 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
    11941 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    12001 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    12061 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    12121 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    12181 cccaatgccc aagcaagcgt caaagtcgct tttttttaat tctccaaagt aacgtaatcc
    12241 tagcacagcc gctgtattat ggagagcatc aaacaaaatg agtccatttg gaaatgtttc
    12301 atgtgtccat tcgggatcac aaaagatata aagatctccc tcatcaaagg aaacagactt
    12361 ttgataagtc tctaagatct tcgaattcgc tggctgaaaa ttcagcaaat aagaatacaa
    12421 attgttctca aatccttctg gaatcagcaa atgactttgc acagaaaatt cttcagatag
    12481 gccaacgatt acttttgtat ggtaaaactg gcgcttgcga ctttgataca cagcttctct
    12541 taaaattttc tcgtatttgc tttgttcctt gtctaagtcc tcaaagatcc gccgagcact
    12601 tgctgttctt cccacaatgc ttccatcatt gaatactagt gtcctaacat cagctggtaa
    12661 atctagttct tcagcatgtg agattgttaa atctgtgatg atcgttcctg gactcccttt
    12721 tgctagttga taagctgtct tcctatcaaa aacttcatga acattattac cgtaaaatgt
    12781 cgtttctaca atcgatcgaa tactggataa cagtggattt ccacttgtga tttcgtttaa
    12841 ttcgagactc tcttttgtac tcatggctcc tcctcattcc ctaaacaaaa aaacgttttc
    12901 tttattatac attattcgag tttttatttg tactgctgcg ctgtgggaag caaacgagtt
    12961 atttttcaca caaaaaaacc gagaacttga ataaacaagt ttctcggttt catcattttc
    13021 cttttatcgg cttagtacca gccgtttgct aaccagaatg ttttagcagc atcccatgaa
    13081 ccataacgag atgttacata ttgttctgct acacgttctt gattggcagc tgaatagtcg
    13141 ccgtttaagt atgaagcatc taattgataa cggccgatgt aacgtccgtt agtagctgtg
    13201 tatgaaccgc ctgattcttt ttgtgcgatc cattcttttg cagagcttga agttggcgct
    13261 gttgcagcag ctggggcagc aggagctact tcaactgttt caacaggtgc aacgtatgta
    13321 tgttcttgcg ttgctgtttc agttgtagat gctgtatttt ctgttgtttt gttttctgtt
    13381 gttgctgaag aatcatttga agttgggatt gttaattgtt caccaacata aatgcggttg
    13441 atgtttgcga tcgaattttt ttcagcaatt gcatcgatca ttgagttgtc tcctgcaaat
    13501 ttttgtgaga tcttagataa tgaatcacct gattttactg tataaacttc atcagcgtga
    13561 gcatttgtag ttcctacaaa cattgcagta gcaccagcag caaaagttgt acctaaaaca
    13621 attttcttaa atgaattcat attaaactcc tttttttctc cgtt
//
LOCUS       DAJSMI010000068        13662 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJSMI010000068 DAJSMI010000000
VERSION     DAJSMI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.068"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            149..1576
                     /locus_tag="ON430_001571"
     CDS             149..1576
                     /locus_tag="ON430_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3219493.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1668..2033)
                     /locus_tag="ON430_001572"
     CDS             complement(1668..2033)
                     /locus_tag="ON430_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR3219494.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2030..2395)
                     /locus_tag="ON430_001573"
     CDS             complement(2030..2395)
                     /locus_tag="ON430_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR3219495.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2587..2835)
                     /locus_tag="ON430_001574"
     CDS             complement(2587..2835)
                     /locus_tag="ON430_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219496.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2834..2962
                     /locus_tag="ON430_001575"
     CDS             2834..2962
                     /locus_tag="ON430_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCR3219497.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3019..4713
                     /locus_tag="ON430_001576"
     CDS             3019..4713
                     /locus_tag="ON430_001576"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCR3219498.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4717..4836
                     /locus_tag="ON430_001577"
                     /pseudo
     CDS             4717..4836
                     /locus_tag="ON430_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5487..6620
                     /locus_tag="ON430_001578"
     CDS             5487..6620
                     /locus_tag="ON430_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR3219499.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6651..6971
                     /locus_tag="ON430_001579"
     CDS             6651..6971
                     /locus_tag="ON430_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCR3219500.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(7000..7464)
                     /locus_tag="ON430_001580"
     CDS             complement(7000..7464)
                     /locus_tag="ON430_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3219501.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7595..8844)
                     /locus_tag="ON430_001581"
                     /pseudo
     CDS             complement(7595..8844)
                     /locus_tag="ON430_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8873..9190)
                     /locus_tag="ON430_001582"
     CDS             complement(8873..9190)
                     /locus_tag="ON430_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3219502.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9216..9536)
                     /locus_tag="ON430_001583"
     CDS             complement(9216..9536)
                     /locus_tag="ON430_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3219503.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(10000..11415)
                     /locus_tag="ON430_001584"
     CDS             complement(10000..11415)
                     /locus_tag="ON430_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3219504.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11432..12748)
                     /locus_tag="ON430_001585"
     CDS             complement(11432..12748)
                     /locus_tag="ON430_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3219505.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12898..13596)
                     /locus_tag="ON430_001586"
     CDS             complement(12898..13596)
                     /locus_tag="ON430_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR3219506.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4329 a         2779 c         2348 g         4206 t
ORIGIN      
        1 attatctgtt ttccaaattt tataacataa aaaacctttt ttaacggcat ttatatgctt
       61 tttcagaaat taactgatga tttcaaaaag aaaacatgtc ataatatttc ccatattgtg
      121 caaaaaaata caaggcggag gctctactat gattgaagaa tatattgaga aaaatatttt
      181 aagacagctg tttttatgtg ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct
      241 ctccaatctc ctgcatgttt gcaaaaccac tttactcaat gatatcaata acataaaaaa
      301 agaatttgaa gaacagatcg cttatacaca tcgggaaaag gaccgttaca ctttatattt
      361 ttcagaacat atacctcgat gcaagatcat gcagcaattg tctcaaaatt cgttgttttt
      421 aaaaacttgt ctgctttatt tggaagaaga tgaacctgat tatcttcaat taactgaatg
      481 tgaattcatc tctgtcagca aagcttattc attaaaaaaa caagtattag cttattttaa
      541 tgactgcggg atcgaaatcg accgctactc ccctcgattc actgaaatgg aaagacgtct
      601 gctgcttctt aatgtgtcgt atcgtttagg cggttttaat tcttgggagc tacctgaatc
      661 gttttttgaa cgtgccgatc gtttcattga atccgtaacg gaaaatagtg gcagatttta
      721 tgataaagaa aataaagaaa tcttatcgat tggttttgct atcagtttcc tacgccagca
      781 agtgtgtgct gtgacaatag attcaaaatt tatcgaagaa atcaaaaaac gtcctattta
      841 caattatgtc gaatcagcat gggaaaacac tgatttccaa acctattata aaaaagaaga
      901 attcgccttt attttgacac tattcaatct ttgtaattat ggtttccatt cttatcagtt
      961 gattgcggaa gactttcaac agctacacca agtgtttatt gacaatactc ccgaaataaa
     1021 agaactagtt gctacctttg aatctcactt caatcaagaa ttattcggaa accaaccgtt
     1081 cgaacgtgcg ctgattcatc tgatgcgttc ggcttgggat aactatcaat tattcatgcc
     1141 agaaaaattt tatcttttaa atgaggaaca gacaaattta tataaagaag tccagacaat
     1201 tttttccagc tggtccagcc agttacctta tgatctacgg ttgaatccta attgcatgcg
     1261 ggcttttgtg atcgaattga gcggaattct ccgtctgaca aaagaacatc tgactatcta
     1321 tatcgtcaca aattcggatg ttcattatct gatttatcgg gaagccttgg aagctgtaac
     1381 tactttcgat tttcaagttg caccaacaat ttattcaagt atatctgata taaagaagta
     1441 tgcgcagcag tcctcaaacc gggtattatg tgaacgtaca ttatatacac cagatgcggt
     1501 ccaatatgaa aatattattc ctatttcgat caatacgatc gatcgagcta tcatttcagc
     1561 tgtgcaaaac aaataagggg ctgtgacaaa aatcatgtca cagcctctta tttgttgaag
     1621 atgcccattt ctgtaacaat ctcttgatac acgtcaggct cacaacctca cttattttgg
     1681 acatctacgg gtccgatctc tttatttaaa aaataagaaa tgatggttcg gataactacc
     1741 aacgccgcta gttggaaaat atcctgcaag gtaggtttga taatcgattc gatgatccca
     1801 gctgaaacaa gaatttccaa acttagaagt atatagcctc ccaacatctt tttaatagca
     1861 ttgttctcgc tgactcggtc gctttgcact tttgtatgga caaataaata tttcaaaaaa
     1921 tccttgattg atagacaaac accccaaacc aataaacaga tagcaaagat atcaagtccc
     1981 agtacaagtt tttcgaaata tggaagaaga aactgcatca actctgtcgt catcctacct
     2041 ctcctcttct ttgtttttct ttgtcttcta tttcctgatt caagaaataa gaaatgactg
     2101 ttcgaatgat aataataagt gcaagcttca aaatatctgt ccaagtcggc ttgataactg
     2161 actcgatgat atcagcaacg actagtactt ccaagcttaa caaaatataa cttgctagca
     2221 gttttcttgt atctgcatta cgagtattac gttctgcata gtttttcttg acatgagacc
     2281 aatcaaaaag tttgaataga acaagaacga tcccgacaat taagataccg atagaacaaa
     2341 tatttaaaat agcaatgatc cattctagaa attcaattaa atttgagcga aacatatacg
     2401 ctacctactt tcttttaatt tgttcctatt cttttattgt aaaagaaagt taccttgttc
     2461 gtctatttaa aagttgttct aatgataacg gttcgcttaa atttttcttt ttcactctcc
     2521 catttttctc cctccatcca taaatcgtgg aagtatccaa aacaacgtag caagttaaaa
     2581 taaggcttag tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc
     2641 caaatttcta aaaatagaac catacacctc ctcaaatctt ttcttcttac ctaccctatt
     2701 tttatttaca ggaaaatcag acacttcttc ttttttgtca aaatttcctt tttcttctcg
     2761 tcctatcatt tggcattcta ataaaaacga actatccttg gatatagaaa gagaaaacaa
     2821 ggaggcgttt ttcatgagta aaaaattaat caaagtcggc atcggtttag gtttgcttgc
     2881 ccttggcgct gcatatctag gaaaaaagac aggactattc gaagatgaca gtcatttata
     2941 tgatgaattc gaatcaatct agcatcctat ttagttgaat cactacggac atacttggaa
     3001 gacattggag gaatcacaat gagaaaaagt aaagcaataa tgattggtgc cggtattgcc
     3061 aatatggcag cggctgttta tttgattcaa gaaggcaaat ggaaagggga tcagatcacc
     3121 ttttattcat tggatgatca cggttcaaat gatggtgcac cgaccgacac tgtagtggat
     3181 gaatattgga ataaaaacca tccattagaa aaccaaaaag gctatattgc tcgaggcggt
     3241 cgcatgctta attaccgtac ctatgtagat ttgatggatc tgttaagtcg aataccttct
     3301 gttactgagc cagggatgac tgctgaagaa gatacgagag attttgatgc aaaacaccga
     3361 acctttgaca aagcacgttt gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg
     3421 ggattcaata ataaagaccg tattcttctg actaaactga ttgctatgcc tgattcagaa
     3481 gaagaaaaac tagacaatat cactatcgaa gaatatttca aagatgatcc acactttttt
     3541 gaaacaaact tctggtttat gtgggaaaca acatttgctt tcagaacacg tagttctgct
     3601 caagaattgc gtcgctatat gcatcaaatg atttatgaat tcactcaaat cgaacatcta
     3661 gtgggcgtca atcgaacacg ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag
     3721 tacttgaaag aacaaggatg caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc
     3781 aaagaaacac cgatgcaaga tgaaatcact gtgactggtt tgaaaattac caatacactg
     3841 actcaagaag aagaatacgt tgtggtcgat gaagatacag cagttatttt cactaatggg
     3901 tccattacag attcagctac tttaggagat tacgaaacac cagctgtaga aaatatggat
     3961 tatggtgcag cttctagtct ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat
     4021 cctgacaaat tctttgctga tcgagatgca agcgaatggg tcagctttac cctgacgacg
     4081 aaaaaccatt tgttgttgaa tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc
     4141 ttgaattcat ttatttcaac cacaccgatc acgccgttag gccaaaaaga tgtcaatatg
     4201 tctatcgtcg tgcatcatca accgcatttt acgtctcaga aaccaaatga aacagttatt
     4261 tggggttatt tcctttatcc aagacgacgt ggcgaatttg tggataaaca gtatattaaa
     4321 atgaatggaa aagaaatgct agaagaactg attggacaac tttcaaaagt agatcctgga
     4381 ccagtgaata ttcgtgaaaa agaaacagaa attttcgaca gcgtaataaa taatatccct
     4441 gtctatatgc cttatgcttc cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa
     4501 gtcattccta aacattcgac caatctggcc tttacaggag aatttgtcga gcagccttat
     4561 cagatgatct ttactgaaca aagtgctgta cgttctggag aaatcgctgc tttccatttt
     4621 gcgggtattc ctatgtcccg tcttgtcaaa acaccgcgtt acgacaaaga tattcctact
     4681 ctagctcgtg ccgcaaaaaa aatgtttgaa taatcaagag gttgtgaaaa agctgccctg
     4741 catcaagtaa taagaacaac caggctaaaa cagcttctta tgtttttgat ttggttggac
     4801 ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttgtga aaaagctgcc
     4861 ctgcatcaag taataagaac acccaagcta aaactgcttc ttatgttttt gacttggttg
     4921 gacttattac cgcagatgca agcttttgaa caccatttac caagaggtta tgaaaaagct
     4981 gccctgcatc aagtaataag agcagccaag ctaaaacagc ttcttatgtt tttgacttgg
     5041 ttagacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa
     5101 ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat
     5161 aaatttgact taataccgag aaactgatcc cttctgtcat aacctctttt tatttatgtc
     5221 tgtttctttt tgaaaacaac tattttttca acgtttactc tcttgccaat ccaacatcat
     5281 ctaacggata caactatttc aatgattacc attacactaa aaaatcggaa acacacctct
     5341 ccgaacacaa atacataaga ctatagaatg tttcatttaa gaaaaaaagc gtttcattat
     5401 caaaaaggta agatctcctt taaaatgaaa ttatctgtag tactaataat gcagacaatt
     5461 ttatgaatct gaaaggagca tctccaatga aagcagtcac ttggcaagga aaacagaaaa
     5521 tggaaatcag aaacgtagat gatccaaaaa tattagaacc aacagatgca atcatccgta
     5581 tcacagcaac agccatttgc ggctccgatc tgcatcttta ccatcatggt gaagcagttt
     5641 tggaaaaagg gtatgttgta ggacacgaac caatgggaat cgttgaagaa gtgggatctc
     5701 aagttcaaac attgaaaaaa gggcaacgtg tggttatccc ttttaatatt agttgtggtc
     5761 attgtcattt ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat ccagatcagt
     5821 tatatggcgg gcttttcgga tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg
     5881 aatatctaag agttccttat gcagactcta cttcatttgt tgtaccagat aatgatcttc
     5941 ctgatgaaaa agttttattt ctatcagata ttttgcccac agcttattgg agcgttgaaa
     6001 acgcaggagt aaaaaaaggc gatacagttg tgattctagg ttctggtcca gtcggtctat
     6061 tcgctcaaaa attcgctgtt atggctggag cagagcgcgt aatcgcagta gatccagtac
     6121 agcaccgtct agataaagcg gctaattaca atggggttga aacttatttc ttagaagaca
     6181 cggcacaagc tggaacagaa ttgtatgaac tggctaaagg tggcgcagat gtcattatcg
     6241 attgtgtagg aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct
     6301 tacaaagcgg cacaatctct ccattacaga tggcatcgca agctgtcaaa aaatttggaa
     6361 cagtccaagt aactggtgtt tatatgactc cagcttcttc ttacccgctg caagaatttt
     6421 ttatgcggga tatcgctgtc aagcatggac aggcaccaat tatccatcta atgccaaaaa
     6481 tttacgcaat gattgcagaa ggactattcg atcctacaca aatcattact cattcgctgc
     6541 cactatcaga agcagcacaa gcatatgaga tctttgacaa aaaagaagat aaaaatataa
     6601 aagttgtttt gaaaccttga attcagtaaa aaatacaggt gattttcttt atgcccaaaa
     6661 tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga agatatcgaa atacgaggac
     6721 gtcgcttccg cttctatttc caaaatcgtt acacattgat ctcactggct gtcgatttat
     6781 taaccgggat tttctatatt tttggaagcc ttgcttcctt aactaaaatc cccgatcgat
     6841 atggcaccta tttttatttg gctggtgcta ttttcttgac gattcgtcct attctcagaa
     6901 tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa
     6961 aagaggcatg agaatttttc aaatctcatg cctctttttt caccctatcg tatcactttg
     7021 atacagacga gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg tccactcgta
     7081 tcgttcataa aacgtggtat gatctgtaat taagtaaagt ttttcgtacc caagttgatg
     7141 catttctttt ctgatttccg ctaatatccg cttggctatc ccctgcttac gcttttccgg
     7201 ttcaacaaac agggcacaaa gatttggtgt cagatcaggg cggttatgga agtcgttttc
     7261 aatgacgccc gctcctccga cgatggtttc tttttcatct aagaccacat accattgagg
     7321 aatactttcc ttattagcga ttccagtacg aatactttcc tcatacacaa cagcaggaac
     7381 tccccatttt gatgaaaacc aattcgcgct ttgttttact aattctgggt gttctcttaa
     7441 tttgatccag ttatatgtaa ccaatgttta aacctccgac taaaataaaa cgtttacaaa
     7501 aattatacaa gtctttatga aaattagcaa tgacaacaat agtgatgtaa aaaaaaaacg
     7561 gaaaacaatc acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc
     7621 tttcttggat cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat
     7681 tgacagcggc tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg
     7741 gtggagttac ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa
     7801 agtaagcaac actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt
     7861 tcaacacgat aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa
     7921 tcgacaagtt tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta
     7981 acagtaaacc taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt
     8041 tgttcacgat aaacggtgcg ctatgcccag ctgcgattgc attaacgttt gcttcaatgg
     8101 ctggcgcatt gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga
     8161 accataagaa tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc
     8221 ccataaatgg ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg
     8281 cagcaaaaat tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg
     8341 aagcaaatgc ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt
     8401 catttccgct taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa
     8461 acaaaccgtt tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa
     8521 tcacacctgc ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagaaagc
     8581 aatcaagaca gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca
     8641 tgattcacca ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt
     8701 gatcattact gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg
     8761 aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc
     8821 cataaatgca acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt
     8881 tcaatcgacg atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca
     8941 tcagatgatc ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt
     9001 cttgtgtcag catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt
     9061 tgattttttc atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg
     9121 catcgctttt tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt
     9181 gatcttccat ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct
     9241 tggtcgagta ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa
     9301 ggaatgcctt taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt
     9361 cctaaaagta aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat
     9421 acagcaaaga tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact
     9481 aggagacttg tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta
     9541 aacacgttcc tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag
     9601 cgctttaaaa agttgatatc atcacattta aggtctatac caagttgcat acacactaaa
     9661 aaagaaagcg cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc
     9721 acaaaaaaag ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa
     9781 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt
     9841 gctattctta ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca
     9901 atcagctgac cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat
     9961 aaccagtaaa tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg
    10021 caaatccaat tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt
    10081 tctgcttcgt tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt
    10141 cattggtgac acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc
    10201 ttctgttgct ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc
    10261 ttgcacgatt cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc
    10321 tgttatcagc caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat
    10381 gattttcgga tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa
    10441 gacttcataa tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc
    10501 gcgaacgcgt ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat
    10561 cgtttctaaa tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag
    10621 gaactcttct gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa
    10681 atcatacata taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc
    10741 aggatttaaa atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc
    10801 tttagccgtt gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc
    10861 atgcggccag cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa
    10921 tgtaaaccag ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc
    10981 ataaaaatca actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg
    11041 gacttcataa tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat
    11101 catttcactt atatgctgag catattcttc atctactata cagttctcat aatcagtaaa
    11161 aaaacgagcc caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc
    11221 cacatcctgc tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg
    11281 ccaaacaaaa tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt
    11341 tcctttccaa ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg
    11401 aatagtaatt ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg
    11461 ctacttttac aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa
    11521 taactgcacc gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc
    11581 aagggaccat tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg
    11641 ttccagtgat aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg
    11701 gcataccaaa aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg
    11761 tgatttgttt catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg
    11821 tgactccaga cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg
    11881 gtacttcttt tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg
    11941 gttcccgtat cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg
    12001 caataaacat caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt
    12061 gtacgacatt gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc
    12121 ccgcagccgc gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag
    12181 aaacattcgt tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc
    12241 agcaataaag ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat
    12301 ctgtatattg tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt
    12361 cataaacagt tggattaaca gataaataag acatcactgc gatgatacca gggaatacac
    12421 catccacttt attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca
    12481 agaaacttag tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac
    12541 gcaagaaact tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg
    12601 tgccaaaagc acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca
    12661 tgatatacct taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat
    12721 ccataaaact cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt
    12781 tgtctattag tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc
    12841 aaatataaaa caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca
    12901 attagtttct ttttctccta ttattttctg taattctctc cgatttattg ccatatggac
    12961 agtcattgca tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt
    13021 atgaatatcg atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc
    13081 aattgattca ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc
    13141 aatcatcgta tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga
    13201 agtttcaatc ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc
    13261 cgctgccaaa cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat
    13321 catccgatcc tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt
    13381 atcactgaca aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc
    13441 ccgcacagct tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc
    13501 attcggcagt ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc
    13561 agtagttact tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga
    13621 tgtatttttt cgtaattatt ataacacgtt tgaatcatct ga
//
LOCUS       DAJSMI010000069        13211 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJSMI010000069 DAJSMI010000000
VERSION     DAJSMI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.069"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(141..213)
                     /locus_tag="ON430_001587"
     tRNA            complement(141..213)
                     /locus_tag="ON430_001587"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Lys,seq:ctt)
     gene            594..1163
                     /locus_tag="ON430_001588"
     CDS             594..1163
                     /locus_tag="ON430_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCR3219507.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1150..2010
                     /locus_tag="ON430_001589"
     CDS             1150..2010
                     /locus_tag="ON430_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3219508.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2200..2904
                     /locus_tag="ON430_001590"
     CDS             2200..2904
                     /locus_tag="ON430_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3219509.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2909..4744
                     /gene="walK"
                     /locus_tag="ON430_001591"
     CDS             2909..4744
                     /gene="walK"
                     /locus_tag="ON430_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCR3219510.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4741..6057
                     /locus_tag="ON430_001592"
     CDS             4741..6057
                     /locus_tag="ON430_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219511.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKVKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6058..6927
                     /locus_tag="ON430_001593"
     CDS             6058..6927
                     /locus_tag="ON430_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCR3219512.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6982..7791
                     /locus_tag="ON430_001594"
     CDS             6982..7791
                     /locus_tag="ON430_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3219513.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7894..8523)
                     /locus_tag="ON430_001595"
     CDS             complement(7894..8523)
                     /locus_tag="ON430_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR3219514.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8693..9985
                     /locus_tag="ON430_001596"
     CDS             8693..9985
                     /locus_tag="ON430_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3219515.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10061..10723)
                     /locus_tag="ON430_001597"
     CDS             complement(10061..10723)
                     /locus_tag="ON430_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3219516.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFGVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10948..11232
                     /gene="groES"
                     /locus_tag="ON430_001598"
     CDS             10948..11232
                     /gene="groES"
                     /locus_tag="ON430_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR3219517.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11283..12908
                     /gene="groL"
                     /locus_tag="ON430_001599"
     CDS             11283..12908
                     /gene="groL"
                     /locus_tag="ON430_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR3219518.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4400 a         2274 c         2838 g         3699 t
ORIGIN      
        1 tttattgtta ttaaaaatta aaaaactaaa atacataccc gaatttcata cccgaaatat
       61 aaaaaatgtc atcacgtaca tctttttcga aactaccgaa gtagccaaag atgtactgat
      121 gacgtgggtt tggcacccaa tgggccgtga ggggctcgaa cccgcgaccc gctgattaag
      181 agtcagctgc tctaccaact gagctaacgg cccgtacaaa aagtacttaa taatagtagc
      241 actaagaatt ttaaaatgca agactacatt taaagaaaat cagaaaaatc tttacttcct
      301 tataggaaat cattcataga ttattcacat ttgctcgcta ttatataagc ataccaacaa
      361 acaaagttct tgcaagagaa caaaccctaa cacttttttc attttttact cctttcccgc
      421 cgtctccccg gcgggtctcc tgttcttaac gataaaagac aagaaggctt agccttcttg
      481 ttttttgttt tcatggaatc ccgctaatca aatcacctag ttaaagcaac tagattgaat
      541 gtttatcata agtctgatat catgtttatg aaatgaaaaa aggagcggtt atgatggaaa
      601 aaatgcgtca gcaattaatg gagtggggga aggaactaga aacattccgt ctgcctcact
      661 gggaagaact ccctgactta gaactttata tggaccaagt cagaacgtta gttgatcgtt
      721 atctttcacc tgtgatacaa ggagaaaagc atccattgct tacctcttct atggtaaaca
      781 attacgttaa actaggtttg ataccagcac cagttaaaaa aagatataac aaagaacacg
      841 tagcattttt acttgcaatc actacgttga aacaagtatt gactattcct gaaatcaaag
      901 aagggatatt gttccaagga aaaacagtag gaattcgtga agcctataat ctattttgcg
      961 atgaacaaga agcagctgtg tggatggtca gccaattagc acaaggtaaa tctcatccgc
     1021 aaaaattcga ccaggcaacc cctgtagaat atattgctgt aagagcggca actttatcgt
     1081 ttgcaatgaa acttcttgcg gaaaaaacga ttgttctaga aaccgaatat ttaaaagagg
     1141 agaaaacaaa tgaaaaacaa taaaatcgca attttagtag attcaggtac ggatgtgcca
     1201 gaagaattga taaaaaaata cgagatgtat atgatcccat tgaaaattat ttataaagat
     1261 caggtatata cagataaaat tgacattacc tctgaagaga tctatcaacg tttgccagag
     1321 gaaatccctg gtacatcttt gccggatggt gagtcgatca cagaaatctt tgagcaaatc
     1381 aaaaaagacg gttatgaaaa ggtacttgcg gtaacaattt ccagtggatt gagcgggaca
     1441 tataatgtgg ttcgtttaat tgcacaacag caagaagaat tggaagcata tgtactagat
     1501 acgaaaaata ttggtattgg tgcaggtttt tctgcgatcc aagcagcaaa atggctggaa
     1561 gaaggagtag aatgggatca actgatcagt aatctgaatg agttagtcga aagaacaaaa
     1621 gttttcttta atgttgcgac attggaatat cttcaaaaag gtggacggat cggtttagtt
     1681 gcttctattg taggaaccgc tttaaagctt aacccaatca tctcatgtaa tgaagaaggg
     1741 atttattaca cagtaggaaa agctagggga cgtaaaaaaa gtttggataa aacggttgct
     1801 tacgtcaaag aaaaaatcgg taatgcaaag gtcttcaact tagctgttgc ccatggggat
     1861 gcgaaagaag aagcaattga aatggaagcg agacttaaaa gagagttccc acaagcaaac
     1921 caaatctact ttggccagat ttctccggca ttagtagtcc acactggtcc gggattattg
     1981 ggtgtcggtg tacaagtttt agaaggataa atcaaaaaaa ggaaacactg aggtgaaaat
     2041 cagtgttttc cttttttatt gaagttagaa caaaaaataa taaagtattt tgcactttca
     2101 ttttaatttt ctcttttgtt ttataggcaa agatgaaaca aaagcagaaa tacggtataa
     2161 tgagaaggta tgacaataat atataagagg tggctcttta tgaaaaaagt tttagttgtc
     2221 gatgatgaga agccgatttc ggatatcgtc aaattcaatt tagccaaaga aggctatgat
     2281 gtttatactg catatgatgg ggaagaagcc ttggaaaaag ttgctgaggt agaacctgat
     2341 ttgattttac ttgatttgat gctacctaaa atggacggct tagaagttgc tcgtgaggta
     2401 cgcaaaactt atgatatgcc gatcattatg gtaacagcca aagattctga aattgataaa
     2461 gtcctaggct tagagttagg cgccgatgat tacgtgacca agccattttc caaccgagaa
     2521 ttagttgcac gtgtcaaagc aaatcttcgt cgcggtgcca cggctgcgaa ggaaccagaa
     2581 gaggcagcac ctgcggaatt gacgattggt gatttgacga tccatccaga agcttacatg
     2641 gtgacgaaac gaggagaaac aatcgagtta acacatcgtg aattcgaact actcttttac
     2701 ttggcgaagc accttggaca agtgatgaca agagaacatt tattacaaac ggtttggggt
     2761 tatgattatt ttggagatgt acgaacagtg gatgtaactg tacgtcgttt gcgtgaaaag
     2821 atcgaagata atccaagcca tccaaattat ttagtgacac gtcgtggtgt cggttactat
     2881 ttgcgtaacc cagaacagga gtaattggat gaaaagaaaa gtccgctttt ttcgatcagt
     2941 caattttaag atcgctatta cctttattct gattttattg atctcgatcg aaattattgg
     3001 tgcctacttt atacgtggtt tagaacgttc cacgatcaat acatttatta aagatatgaa
     3061 tcaaacggtg gaatcattag caacaacaat cagcccggaa ttgaatcgaa aagataatgc
     3121 agatgatgaa gaggtaaatg cgaatatcaa aagatttatt gaaaacagtg cgactagcga
     3181 tatcatcgaa atacgggtcg ttgacgaaaa agggatcatc cgaggaacaa ccgatgtcaa
     3241 tgaacaaagc gctgtgggaa agaaaaatga ttatgttgat atcaatgatt tcacgactaa
     3301 acgttatgtt gctttagata acgacgataa acgggtgaat atcaatgttc aaccgatcct
     3361 gtccccgacc ggcgatgccg ttatcggggc tctttacgtc aaaagtaata tcgaacaaaa
     3421 atattcggaa atcaataaca cagcagtcat tttctttact gcctcactaa ttgcggcatt
     3481 tatttcaatg atcgtatctg ttttagttgc tcgttcgatt acacagccga tcggtgaaat
     3541 gagagaacaa gccattcgaa tcgctagagg agactacagt cggaaagtca aagtgcatgg
     3601 acaagatgaa cttggacagc tggctgatac cttcaatcag ttagctgaac gtattgaaga
     3661 agctcaagat acaatggaag ctgaacgcaa tcgtttggat agcgtgctat cccatatgac
     3721 agacggcgtc attgcgacag atcgcagggg taaagtcatt acgatcaatg atatggctgt
     3781 ttccttgcta gatatcaaaa acgaggaagc aatcggacaa tcgattttga cacttttaga
     3841 tatcgaagaa gaatatacgt tgcggaaatt attagaagat ccaaacgaaa tggtacttga
     3901 ccgttcaacc agtacattgg aaagtgatca gatgatcttg cgagtagact tttctatgat
     3961 ccgtcgcgaa tcaggtttta tcagcggatt agtagctgtc ttgcatgatg tgacagaaca
     4021 agaaaaaaca gaacgggaac gtcgtgaatt cgtctctaat gtttcccatg aacttcgtac
     4081 accgttgaca agtatgcgta gttatatcga agcattgagc gaaggtgcgt ggaaagacga
     4141 ggaagtagca ccaaattttt tgaaggtaac gctagatgaa acagaccgaa tgattcgaat
     4201 gatcaatgac ctcttaaatc tatctcgcat ggatacaggt aatacccagc ttcaattaga
     4261 atatgtcaat ttcaacgagt tagtgaattt tgttcttgac cggtttgata tgatggtggg
     4321 aaatcaggaa aaaaattata agatccgtcg tgaatttacg caaagagatc tttgggtcga
     4381 agtagataca gataaaatca tccaagtagt cgataatatc atgaataacg cgatcaagta
     4441 ttcaccagat gggggaacga ttacttgtcg cttattagaa acgcataata acgtggtttt
     4501 aagtatcacc gatcaaggat tggggattcc taaaaaagat ctgaaccgag tgttcgagcg
     4561 gttctatcga gtagataaag cacgtgcaag agcacaaggt gggacaggac ttggattagc
     4621 tatttctaga gaagtgatta aggcgcatcg aggcgctatt tgggcagaga gcaaggaagg
     4681 aaaagggtcg actttttata tctcgctgcc atatgaacct tatgaggagg aatggtggga
     4741 atgagaatct ctgaaaaagt cgttcgtata ggactcattt tgatggttgc gttaagcata
     4801 tacttctctt atgcaatctg gctgagccca gcaggaaaaa gttcaatcaa cgtagatgag
     4861 acaaacactc aaatgatcga gtctcaaagc aatcgaaaaa cttccgaagt ctttcttccg
     4921 cttcatgtta catggcacca ttcgggaatg atccaagaaa ccaacagtga aaatctcgtg
     4981 taccgattgc aattgatcat cgataaagca agatttggaa agctttctga agtagtttca
     5041 ggagacaaat cacagtttga acaggtgaaa aatctgaact ctggttttga gttggcctat
     5101 aatgcgcctt ttttgctaag agaatacaag gaagcatttg atttagattt agatgtgtcg
     5161 aacttggcaa aagaaagcga aaatatgtat tttactagaa tacaattcga tcaaggaaat
     5221 aaaaaaatcc gatttttgaa ttattcgaac catctcgtat atgaagcaaa tatttcgttg
     5281 aattggacag atatcaataa agaattaaca gcaaaagaca caaaatggac agaaatgaca
     5341 acagaaccat cgacgatcga cacgcaatat gctacgaaac attcgatcaa actgaaaaag
     5401 tatagttata tcctgtcaca gcaaccctat acacttttta gaaatgcttt tttccagaaa
     5461 ccagatagcg tgaaaaataa tgatgaaagc aatgaattga ttttttacgg tgggaatgaa
     5521 acaatgtcta tccattcgga aacgcaagta gttgattttc gtggagagct gccagagaaa
     5581 aaagaaaatg ctagtgtatt ttctgaaagt ttcccctata ttagcaaatt aggcagttct
     5641 ttagggaacc tcagatattt tgaccgcagt gacaatacga tcgattatcg gatattcgtg
     5701 gaaggcttcc cggtttttgg gacagacagc agaggacaga ttggtgtgga agttggcggt
     5761 gaaacgaatg gttctgtgca agtgaatatc caaaccagtt tgaatacgat tcaagtgccg
     5821 ataccttcag acgaagaagt agaacttgca agtacgaatg aaatcatcca gcagttaaaa
     5881 gttaagggag cagatagcaa caagattggt tcagtgatcg ttggctatac atggcaaaat
     5941 gtcaaagaaa cgaatcgtgt cgttgatctc ttacctgaat ggtatgtgaa atataaagat
     6001 aactggtatt cagctaacga ccttctggaa aaattacctg gattggaggc gaattaattg
     6061 gatttcaaaa aaatcgaatg gatatttttc gtcgcttttt tagggttgaa tgtgttttta
     6121 ttcagcagtt atcatgacgc ggtataccaa gaaaacaatg tcattcgctc taatcagact
     6181 gaatcaatcc aacaacgtct ggctagcgac gatatcactt acacggggaa gatttcgtct
     6241 gagaacaagc aaggctatta tcttagcgcg gagcagacca atcttagcac ttcattagcg
     6301 aattatcgca aggctggtaa aagcggcttg ctggcaagtg gtgtgttttt agacggtgat
     6361 gttttgacga aatctttatc cagttcagat gctgaaaaga acgtgatcga tccagataaa
     6421 attagtagtt ccttggaaga ttttttatct aacaaagaca atattttatt cggtaatgat
     6481 tatgtgtaca cgaaaaactt ttctcattta gaagaagaac cactagaaat ccaagcaagc
     6541 caagagtatg aaggcatccc attcaatgat gatacagcaa aaatcgttct ttctttagaa
     6601 aaaaacgata atgattatag catcacgaaa tacatgcaga cgcatttatc tgatatcgag
     6661 cagctgcgcg aaaagacgga actgtatacg gaagaggaag caatcaatac attgtacatc
     6721 aataacaaaa taccaagagg atcaaaaatt ttatggcgtc agctagctta ttcctgtatt
     6781 ctgaaagtcc gggaaaaaaa tgtgtatgtt cctgtttggc atgtagcgat cgaaacaagc
     6841 gataaagtgg tccaaattga gagtgtcaac gctttcagca atacgatcgt gacaaacaat
     6901 actgtaccaa aagtggaaga tcaataaaaa agcgagtata atgaaaaaag tttacaaagt
     6961 aagaagggaa gtaggagtca aatggctgaa gaaaaaccag cttttaaaat cagtatccta
     7021 gcaagtggaa gttcgggtaa ttcactttat atcgaaagtg aaaaaaaacg gcttttggtc
     7081 gatgcaggac ttagcggtaa aaaaatcact tccttgatgg ctcaaattga tcggacaccg
     7141 gaccaattag atggaatact tgtgacgcat gaacatcgag atcatataca tggcgtgggt
     7201 gtcttggctc gtaagtatca tttggatgtt tttgccaatc agcagacttg ggaagcgatg
     7261 gaaccactga ttggaaatgt accagtggaa caaaaacatc tatttgagat ggggaaagtc
     7321 agaacattcg gcgatatcga tattgagagt ttcggcgtct cccatgacgc agcggctccg
     7381 caattttatc gtttccataa agaaaatcgg tcattcgtca tgttgacgga caccggatat
     7441 tgcagtgatt atatgcgagg tgtcattgaa aatgccgatg gttatttgat ggaaagtaat
     7501 catgatcttg agatgttacg catgggacct tatccgtgga atttaaaaca gcgtatttta
     7561 ggagatagag gacatctttc caacgaagac ggtgcacttg tcatgactga tgtgatcggt
     7621 aatcggacca aacgaatcta tcttggccat cttagcaagg aaaacaatat gaaagaactt
     7681 gctcatttaa caatggaaaa tgttttgaaa gaaaaagatt ttggcgttgg acatgatttt
     7741 gaaatctatg atacagatcc agatgaagca actgagctat tttcgattta aacagttcaa
     7801 tcaataaaaa tataaaaagc agaagataaa atcaatcagg gttggtttta tcttctattt
     7861 ttattttttt agcatattaa aaaaataacc gtattatcga ctgactacac tgatgtgctg
     7921 cttttcatgt ttctcatcca gtatctcatc tactaatgca atcgcgtaat ctgccatgct
     7981 gatcgtactg tcgccagcag cgtttctttg taaataatca tcacttaaca aataagtacc
     8041 tgtacgtttg ccatgtggat caaataaaga agctggactg atataagtcc aatgtagatt
     8101 tttagattgt ttcaatttta acaatccttc gtacatattg aaagctgttg gatagaaggg
     8161 tgcattttta ggtacttggt caatcatacg agtcgtcttt tctgtatcta agaataacga
     8221 agaggcaccc ccaacgacaa ttaagcgtgt atcggttcct tcaagcatct caatcaaatg
     8281 ggacaaagaa gtttgatgca tttcttcttt tcctttggga gcgttgaaag catcaacgac
     8341 aacatcaaaa gatgcaagat cagtagttgt caactcaaat aaatcctttt gtataaacgg
     8401 aacatctgat ggcaattttt ctggatgacg cacgattgcc gtcacgtcta aatctctgga
     8461 caatgcttcg tctaaaataa atgaacctgc atggccagta gctccaatga gtgcaatttt
     8521 cataaaaaac ctcctttttt attcctgtcc attgtaagca gttgctgaat attcgtcaaa
     8581 gaatccgatt tttgaaaagc tcagtgcgca ttcctttcat ggttcgtgat cattgggtat
     8641 agttaaatta agaaaactga ttgataaaaa tagaagttga aggagataag aaatgaaaga
     8701 gtggttagat cgtgcctttt taccaatgat aacatctgtt gtgaatctta gacccttaac
     8761 tgctttgcgt aatggaatga cttacacgat cccaataatt attgttggct cgctgttttt
     8821 cttatttgct cattttcctg tggattacat cgctcagtgg atagacaaaa caggattttc
     8881 tatgtatttt acacaagtat accaggctac atttgatgtg atagcctttt ggatcgtttt
     8941 tgccattgca tacgcttatg caaaagaaga agacatcgat cgcttaccag tcggcataac
     9001 agccttgtct gcttatctac tgcttattca gccactaaat actcagatag atactgtctg
     9061 gacaggtgct gaagggatac taggagcagt tacaactggg ctcatcatcg gatggggcta
     9121 tgcacgttta gtaagacttg cgaaagggtg gaagcaaaga gagaagatac ctgaaaatgt
     9181 ttggcagtct tttacttcat tgattccaat cattgttata gtcactttta cgcttatcgg
     9241 ttcagcggtt tttcaacagt tgttcagaat gactgttgtt caaggagtat ggacatatgt
     9301 ccagcttccc attcaatcgt tcatagacac gttgtttggt gtgcttttat taggtttttt
     9361 tgttccgttt ttatggttat ttggtcttca tggatctgcg atggtcaatg ggttggttag
     9421 tcccgtcttg caagcgaatt cattagcgaa tgcagaaatc ttagcctcag gacaagagtt
     9481 gacggtggca aatggcggac atatcgttac tcagcaattt ctcgatcagt tcatgacggt
     9541 tacgggtgct ggattgacgt taggggctgt attttttatg acggtttttg cgaaatcgaa
     9601 gaaatacaga gaattaggaa atcttgctct tttaccagcc tttttcaaca tcaatgaatc
     9661 aattattttt tccacaccga tcgtgatgaa cccgatgatg gctgttcctt ttattttcgc
     9721 accggtcgtt tcaggattga ttacctattc cgctttatat tttggattcg ttcctttatt
     9781 tacagcagtc caagtccctt ggacaacacc gccgatcctc tcgggtttct tagtaggcgg
     9841 agtgcctgca gcgattttac aaggtatcgt gttgtcaatt agttttttca tttatttccc
     9901 atttttgaaa aaaatagatt ctgcaaactg caaaaaggaa agacagacaa aaaatgatgg
     9961 agcagcagaa aagatgaacg actaaaaaac aaaaatactc ctgtcgcttt agattgggaa
    10021 aatcaccaat ctaggatgac aggagtattt atagtcatgt ttatactagc cctttttgga
    10081 cagctaattg aacaagaaca actaatgtgt tcattcctac gtgtgtgatc atcgatgtcc
    10141 agatccgacc agttgctttg tactctaggc tgaagaagaa acctaggaaa aagtagatta
    10201 gcagatgacc atcattatga gcaaacgcaa acagcaaaga gctgatcact gctccgaccc
    10261 caaaatttga atatcgactg atgatcccca gtatactgcg gcggaatacg aactcttcca
    10321 tgatgggtcc agcaacagtt gtcgccaaaa taaaagcggg tgcttccatg atcatctgga
    10381 tgatattttg tgtattctcg gaaggggttg ctttgccaaa caatacagat tctaacatga
    10441 cagcgatgct ttgcagaatc aatgcgacaa agattccaac caatccataa ataacaatct
    10501 ttggagcaga agcttttttt accgctgatt cgatcgctaa gggttcacgt tgcttaaaat
    10561 aaaaaatagc taggacaacg gcgcctagta tatacatgac tgcagttgct gtgatcaatt
    10621 cagacgtaga ttttacaagt cctatcgaag ctaggatcaa gggtgagata aaaattagac
    10681 catagcaaaa aatgctgagg aagctatatt tttttaaaga catgtaattc ctccttttaa
    10741 gggataacaa gcatgcaagg caagcttata tcgattgatt tcaattatat cataatttca
    10801 ataagcagct gtcagaataa aatttctttt ctttgattta aaagcttgca aaaaagaggg
    10861 agaatgagta ttattaaaaa tgtaggttag cactcgaagg ttaagagtgc taacagatta
    10921 aaaaaagagt tatcggaggg atttatcgtg ttaaaaccat taggtgatcg cgtcattatc
    10981 gaagtcgcaa aagaagaaga aaaaactgtc ggaggaatcg ttttagcttc tgctgctaaa
    11041 gagaaacccc aaactgggac agttgttgca gtcggtgaag gccgtttgtt agaaaacggt
    11101 gaaaaagttc ctgccgctgt aaaagcaggc gaccaagtta tgttcgaaaa atacgccggt
    11161 acagaagtaa aatacgaagg aaaagaatat ctgatcgtcg caggaaaaga tatcatggcg
    11221 attgttgagt aaatttggaa gataaaaaaa taatcaaaaa taatcaatga ggtgaatcaa
    11281 tcatggcaaa agaattgaaa tttgcagaag acgcacgcgc agctatgtta cgcggggtag
    11341 ataaattagc agatacagtg aaagtaacat taggacctaa aggccgaaat gtcgtactag
    11401 aaaaatctta cggctctcca ttgatcacaa atgatggtgt gacgatcgca aaagaaatcg
    11461 aattagaaga tcattttgaa aacatgggtg caaaacttgt atcagaagta gcatcaaaaa
    11521 caaatgatat tgcaggagac ggtacaacga cagcaactgt tctgacacaa gcaatcgttc
    11581 gcgaaggttt aaaaaacgta actgcaggtg ctaatcctct aggcattcgt cgcgggatcg
    11641 aattggcaac aaaagcagca gtcgaagaat tgcataatat ctcaactgtt gttgattcaa
    11701 aagaagccat tgcacaagta gcagctgttt cttcgggttc tgataaagtt ggacatttga
    11761 ttgctgatgc aatggaaaaa gttggaaacg acggtgtcat cacaatcgaa gaatcaaaag
    11821 ggatcgaaac agaactagat gttgtagaag gtatgcagtt tgatcgtggt tacttatcac
    11881 aatatatggt aacagataac gacaaaatgg aagctgttct agaaaaccca tatatcttga
    11941 tcacagacaa aaaaatctct aacatccaag atattctgcc attgttagaa caaatcttgc
    12001 aacaatcacg tccattgttg attattgctg acgatgtaga tggtgaagca ttgccaactc
    12061 ttgtattgaa caaaatccgt ggaacattca acgtagtagc tgtaaaagct cctggtttcg
    12121 gcgatcgtcg taaagcaatg cttgaagata tcgctatttt gacaggtggt acagtaatta
    12181 ctgacgatct aggtttagaa ttgaaagatg taacaatcga aaacttagga aatgcttcta
    12241 aagttgttgt agataaagac aatacaacga tcgtagaagg ttctggagaa aaagaagcaa
    12301 tcgaagctcg tgtgcaattg atcaaaaacc aaattgctga aacaacttct gattttgacc
    12361 gcgaaaaatt acaagaacgc ttagctaaat tagcaggtgg tgtagctgtt gttaaagttg
    12421 gtgcagctac tgaaacagaa ttgaaagaat taaaattacg cattgaagat gcattgaatg
    12481 ctacacgtgc cgcagtagaa gaaggaatgg tctcaggtgg tggtacagct ctagtgaacg
    12541 ttatcagcaa agtttctgct gtagaagcag aaggagacgt ggcaacaggt atcaagatcg
    12601 ttgttcgtgc tttagaagaa ccaattcgtc aaatcgctga aaacgctggt tatgaaggat
    12661 cagtgattgt tgataaattg aagaacgtgg aactaggcac tggtttcaat gctgctactg
    12721 gagaatgggt aaacatggtt gaagcaggta tcgtagaccc aactaaagta acacgttctg
    12781 ccttgcaaaa tgccgcttcc gtttctgcct tgttattaac gacagaagca gtcgttgcag
    12841 ataaaccaga accagcagcg ccagcagctc ctgcaatgga tccatcaatg atgggcggta
    12901 tgatgtaaga aaaagcaact tgcttatcta caactgatca ttagtgaaag acagataaaa
    12961 agaggctgga acagaagcgt ttaactccaa gaaataagaa gaaattcacg gaaattgctt
    13021 ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt ccgctgttta
    13081 tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtcccacgct cttttttgtg
    13141 tgtaattata atgaaaatag ttatttttaa tagttataat attttatcta ttaaaagtaa
    13201 atatccattc a
//
LOCUS       DAJSMI010000070        13102 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJSMI010000070 DAJSMI010000000
VERSION     DAJSMI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.070"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..102)
                     /locus_tag="ON430_001600"
     CDS             complement(<1..102)
                     /locus_tag="ON430_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR3219519.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKF"
     gene            complement(350..1186)
                     /locus_tag="ON430_001601"
     CDS             complement(350..1186)
                     /locus_tag="ON430_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCR3219520.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1197..2126)
                     /locus_tag="ON430_001602"
     CDS             complement(1197..2126)
                     /locus_tag="ON430_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR3219521.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2318..2671
                     /locus_tag="ON430_001603"
     CDS             2318..2671
                     /locus_tag="ON430_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3219522.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2745..3401)
                     /locus_tag="ON430_001604"
     CDS             complement(2745..3401)
                     /locus_tag="ON430_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR3219523.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3627..5561
                     /locus_tag="ON430_001605"
     CDS             3627..5561
                     /locus_tag="ON430_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3219524.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5647..6870)
                     /locus_tag="ON430_001606"
     CDS             complement(5647..6870)
                     /locus_tag="ON430_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3219525.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6864..8762)
                     /locus_tag="ON430_001607"
     CDS             complement(6864..8762)
                     /locus_tag="ON430_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCR3219526.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(8756..9874)
                     /locus_tag="ON430_001608"
     CDS             complement(8756..9874)
                     /locus_tag="ON430_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3219527.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(9957..10790)
                     /locus_tag="ON430_001609"
     CDS             complement(9957..10790)
                     /locus_tag="ON430_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3219528.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(10783..11700)
                     /locus_tag="ON430_001610"
     CDS             complement(10783..11700)
                     /locus_tag="ON430_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3219529.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(11723..11902)
                     /locus_tag="ON430_001611"
     CDS             complement(11723..11902)
                     /locus_tag="ON430_001611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219530.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(11927..13051)
                     /locus_tag="ON430_001612"
     CDS             complement(11927..13051)
                     /locus_tag="ON430_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3219531.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4097 a         2637 c         2164 g         4204 t
ORIGIN      
        1 aaatttccaa ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc
       61 aaaaaccaaa ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg
      121 atattttact atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg
      181 taattctctt cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg
      241 ctgcttctaa aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg
      301 acgtccttgc gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac
      361 gatacacata ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg
      421 ggatttcacg cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc
      481 attcatcatt tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg
      541 aatctgccac acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt
      601 tcggtgtttg gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt
      661 aattataaat tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct
      721 tatccccata cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat
      781 aaatactggt atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt
      841 tacttactgg gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg
      901 gaaacaacgt ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat
      961 aaggttttga caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt
     1021 ttttgaccca caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa
     1081 tagctaagta gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt
     1141 acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa
     1201 ataaataatg tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag
     1261 cgtcctgtaa aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag
     1321 accaaaaagg cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac
     1381 gcatctgctt ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct
     1441 aaaatctttg ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca
     1501 gctgcgatta atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa
     1561 tgtgtcaaca catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt
     1621 tgttcttctt catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct
     1681 ttcaatacag cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa
     1741 aagctgccta tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg
     1801 gaaaaaatca cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga
     1861 actggtacgc cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa
     1921 ggaaagaaaa atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc
     1981 catctcttca cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct
     2041 aataatacga aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa
     2101 accgaatttg gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct
     2161 ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc
     2221 ttatttttta ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct
     2281 catacatatc acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac
     2341 tgaagttcaa gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa
     2401 ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat
     2461 tccggcactt catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct
     2521 gactgcgcca gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt
     2581 catcgacttt aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct
     2641 ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa aagcgattga
     2701 acaagtgaaa gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt
     2761 tcggaatgcg catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc
     2821 aaatcaacat tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc
     2881 aagattcctt tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg
     2941 ggtatcgtta aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc
     3001 attggataaa cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa
     3061 gcacaactga tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc
     3121 aagttcccta caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca
     3181 aaaaagtgca taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa
     3241 taagaaaaat ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt
     3301 gaagacactt ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc
     3361 agtcttttag ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt
     3421 gtctaattac atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca
     3481 ttgctcagat attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc
     3541 gcagtataat ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga
     3601 attcacaaag tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc
     3661 attttggtgc agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg
     3721 tcgctttagt tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa
     3781 ttgaagcacc tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg
     3841 ctcaggacac aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg
     3901 aacaagttag acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta
     3961 cgccagacac acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt
     4021 tttcagatcg aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta
     4081 aattcgattc ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac
     4141 gtttagcttt agctcgcatg cttttgcaac accctgacat tttgattcta gatgaaccaa
     4201 cgaaccattt agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg
     4261 gtgcattatt gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct
     4321 atgaattaag cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt
     4381 tgaaagcaga acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc
     4441 ataaattaga agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc
     4501 aaagccgcag aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa
     4561 aacaagcaaa ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg
     4621 aagatgccgc tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc
     4681 gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga
     4741 aatcaatcat tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca
     4801 ccatcggcta ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag
     4861 aattgtggga tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt
     4921 tcttattttc tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag
     4981 cacgggttgc tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac
     5041 caacaaacca tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg
     5101 acggcacact gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag
     5161 tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag
     5221 agaaaaagca ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg
     5281 ctcctgctcc cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc
     5341 gtagccttca acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga
     5401 tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca
     5461 atgagctgaa ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat
     5521 gggaagaact aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa
     5581 accattcatg ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta
     5641 cctttttcac caataagaag accaattact agtcattctc actaaagctt ctaaataata
     5701 gtaatcaccc caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga
     5761 ctgtgttaaa aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat
     5821 aattttccaa acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc
     5881 tattaattct aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct
     5941 gctagaatca tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat
     6001 aaaataattg gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata
     6061 acttaatgca aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact
     6121 ataaccttga tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt
     6181 atgataagtt gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg
     6241 agcagctttt ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag
     6301 attcatgcaa caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca
     6361 agcttgtata actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat
     6421 tgaagcttcc ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga
     6481 atacaaaaaa ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa
     6541 atctatatct gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa
     6601 caaccaatta attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata
     6661 acgtcctttt ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat
     6721 taacctctcc gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa
     6781 ttctttataa cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt
     6841 atcaagcatt tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt
     6901 tggatagtta gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct
     6961 aaactataat cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt
     7021 ccaaatgtac cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg
     7081 ggcaaaccca taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata
     7141 cctgaaaagc cagcttcact caccctatag ttttccttgt cttcttctat gattattcct
     7201 tctttctcag gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct
     7261 aattctactt tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt
     7321 tttgcctgta aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct
     7381 cgtacattat ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg
     7441 gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct
     7501 ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa
     7561 tatttttcat tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat
     7621 gtatgtccat tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca
     7681 cgaataggca taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac
     7741 aataaaaaaa tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat
     7801 gtcaattgag ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt
     7861 tgcatccacc atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc
     7921 aaaagatcgg cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg
     7981 cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa
     8041 aaatcaatag ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta
     8101 taaatcaaac cataataatg aaatgcaaaa ggattataat aatcttggcg acctcttgaa
     8161 ctgtctcgat accagccatt ttgaagataa caattttcta tattatcaaa tgcttctttc
     8221 agtgctgatt ctttaactgg ataaccaaat ttttggataa ttgttccgac aattatctta
     8281 aaaaattgcc aattaccatc tgcacattca tattcaaaaa tttgcgagaa ccaatgaata
     8341 atctggcttt tttctcctgt atctaatttt ttccataata attgttcatg atgatataac
     8401 gctaataaca ttgcgggcat ttctaccata cgctggtctc tttcatgtat ttctccccaa
     8461 taatcgggat gggaaggatc tgttccagct tttattcctg atagaattaa tttcaaccat
     8521 cgttcatcta attggtcaat tgcgggcccc gctccccata aaacccgaga gaatccttcc
     8581 attcctgcaa ttttatttcc atatcctacc ccttgatgta tataatcaat ttgtgcgcca
     8641 cttgctcgat aaaacggctg gagtggttga tttatctttt taaataagtc aaagtaatcc
     8701 tgtctggtcc ttaatagtgg tactggatgt ttcattgtat tttctcctct ttttactacc
     8761 aaatagctaa ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa taatcagaat
     8821 agatcagtgg cacattataa gtcagttctc tatggtatgc ttctgctccg cctccaatga
     8881 tcccatcttc atccatattc caatttgcat agtgcttttc tgtcttttgg agtatattga
     8941 tagcagcttc atgatacatt tttgatcgta cagaggaaac atatcctgct aattccagaa
     9001 gaccgcatgc agcgcatact ccagcactgg tatcaatatt cgaatctttt acagcaaaat
     9061 caatgagagg aatataacca gttttttcga ttttctgaat aaaatttact gctatctttt
     9121 ctgctatagc tagatagtac ggattttttg tgtaacgata acttaacgcg aatccatata
     9181 aagcccatgc atttcctctg ctccaacaag aattctcatt gtatccttgc ccgccaagta
     9241 accgaatata tttccctgat tctgcatcaa attctgcgat atgcccggtt gatccatctt
     9301 tcctcactag atacttagct gttgtatctg catgctgccg agcaatttta gagaacctcg
     9361 gatcaccagt ttccctcgaa gcccaataca gtaatggtaa attcatcata ctatcaataa
     9421 tcatccaacc aggtttatgg tcattccatg ctactaaata gttccctgaa atattatagc
     9481 gtccagcaag taccgtagct gcatgtaacc ctagttgtcg actatgtttg ttttgagact
     9541 gttgataatg tgcaatagcc gatggtaaga ataaaaaacc tacatcatga tgaacgtctg
     9601 aaaaatggta cagggcagat tctaataatt gttcttgttg tattgctgcc tctttgaact
     9661 tttctcgttt tgtcttgtga aagagctgcc ataaaatacc actccaaaaa ccattggtcc
     9721 accagtcgat tcctctgtcc tttattacgt ctgtatattt tccgttgtaa gaaatatatg
     9781 gaattttatt ccccattctg tctaattccg catctatctt ttcagaagct acattcaaaa
     9841 tttttcttga ccattccttc gtctgttcat tcatggatat cccgccattc ttttaatttt
     9901 tatgaatgtg gagttgctcc tagaacgaac tagatggtta acattcgtta ttttttttac
     9961 ttatttgcag ctccaatgtc attctcctta aatttagact gcgcccacac ataaatcaag
    10021 accattggga tcactgaagt tgctaaaatt agcataattg cgccataatt aattggctca
    10081 cctgtactag aaaccgtcat atttttgata attaagggaa ccgtatattt atctttatct
    10141 gaaagcatga ctaatggaaa tagatattga ttccaacttc ccataaagat aagtaaacca
    10201 ctagtaataa tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat cacccactca
    10261 ttggcaccat cgattctggc agcctccatc agtgctgtag gaaattgact gctatattgt
    10321 cgcatcataa acactgaagt tgtactggca atcgaaggga tgatcacacc tgcataagta
    10381 ttcgataatc ctaaacgact cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc
    10441 accatcctag atagcataac tgtattgaac aacagtttat tccccataaa acgatacttt
    10501 gctaatgcat aacccgccat tgtagaactg gctgtagcta aaactgtact gattacagca
    10561 ataacaatcg aattccaaat aatcaaggga taattatatc tcgccatgat ggcttgccag
    10621 ttttttaata gcatatcccc aaacttgaaa ttgattccgc cacttaaaat ttcttgatta
    10681 ctttgcgttg aagctacaat catatataaa tatggcatca aaaatattaa ggcaccaaac
    10741 acaataatga cgtacgtaaa aattctgctt tgtatctttt gtttagtcat ttttatcacc
    10801 tccgaattta aattgaatag ccgataatat aacagataca aacaccatcg tccaggcaat
    10861 agcagaggct tttccaaaat caaccatttc aaaacttgtt ttatataaat tgactcctac
    10921 gaccaaagct gaattttctg gtccattgga agtcccaaag aatatatttg ggatattgaa
    10981 attacccaag ccattgatag tggctaaaac tgtggccatt aaaatgattg gctttagcat
    11041 ggggatcgtt aataaaaaga attgccgaaa tttcgatgct ccatctattt cagcactttc
    11101 gtatatctct ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt tatatcctac
    11161 actaagccaa atcatcgtga tgatcattga tattcgtatc caaaaaggat ttgttaacca
    11221 ctgtacaccg ttaattccta gcgcattcaa aaatccattg aataacccag tcggattgaa
    11281 aaaagtcatg aatacggaag ctacagccac cgttgatgta atggttggaa tataatagac
    11341 agttctaaaa aatccttttc cttttaactg tgctgaattc aataaaacag caaagtaaat
    11401 agctaatagc atcgttattg gaattgatcc tgccatgata atcaaaacat tcgtgtaaga
    11461 tttaataaac aatggatctc ggaagatagc tagataattg tcaaatccaa caaattcaaa
    11521 tgagtagcca attttcataa aactaaaagc aaacgagata atgatgggaa tcaaaatcgt
    11581 cacaataaag aaaataacaa aaggtgtgac caatccataa gcactttttt tatgagacgt
    11641 atctgttgct tttttcttgc tgttctttat tgtcttgctt ggtaacgaag attgcatcat
    11701 cgttaagtcc tcctaatatt ttttaatcac cagttaattt tacgccgtac ttatgagcaa
    11761 aattttctgc ttcttttaga tatgaatttt caaaatcacc tgtttgccaa tagttatatg
    11821 cagcggtagt caaataagac gtagcatctg aataagtggc tggtaaaaca gtatttgatg
    11881 tattttcatt gattttattc aatagttcat agtatttttg tcctccttag tagtcaaact
    11941 ttttctcagc tgcttctgtt tgataggcca atgagttaga tgtagccacg cccagctcca
    12001 gtgcgttggc taagcattct tcatctgtca gcataaatgt aagaaattgt tgtgccactt
    12061 gtggattttt ggcatcttta gggacataaa aacttgatcc tcctgatact gcaccttttc
    12121 ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca gatgcatcag
    12181 gaaaattcaa agacatattc gtagcatacc agccgccagc aacaaacatt gcagattctt
    12241 gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac aatatctgag tcgatcattt
    12301 ctttgattaa ctttgcagca tgtttcgctt ctttagtatt cagacggata tttccttctt
    12361 catcaagcaa agattgattc tgctgggcta gcatgttgat aaacaattct tggtccccag
    12421 attctggcaa ggcaattcca aaactaccag ttttttcttt taatgctttt gccacctgga
    12481 tatattcttc ccaagaactg atttcttcaa aatctacacc tatttcagca aatttttttg
    12541 gttgataata acaaagtgct ggggtaaaat cactagccca aggaataatc gtttcattac
    12601 tcgtttgttg acgcaaaata ttcaattttt gatcataaaa ctgatcgcca tatttgtcat
    12661 aaaatccata ttcatcagca ctataaaaag catcttgaaa tttttccagc acacctgctg
    12721 ctgaagtgtc tgccatggag ataatatccg gcatatcttc gtgtgttacc aattttgggg
    12781 tgatcatttg tactgttgct gttcccgaag ctacattcac aatttttaag tcaatatttg
    12841 ctccagtttt gcggttatag atttttgcag cattttcaac atagtttgtc agtttgccgt
    12901 ttgcgacata tacttctaat gtagtggctt tttttatcat tggatctgcg atgtttgctg
    12961 ttacttcttc gttagttttt ccacatgcac ttaagctgaa taatagggta atgtttagca
    13021 aagctatcac caatttgtat ctttttttca tgtctatccc ttctttcttt tcaagttctt
    13081 atataaaacg ctttcactta tg
//
LOCUS       DAJSMI010000071        13037 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJSMI010000071 DAJSMI010000000
VERSION     DAJSMI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.071"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(37..1488)
                     /gene="cls"
                     /locus_tag="ON430_001613"
     CDS             complement(37..1488)
                     /gene="cls"
                     /locus_tag="ON430_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR3219532.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1829..4495
                     /locus_tag="ON430_001614"
     CDS             1829..4495
                     /locus_tag="ON430_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3219533.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4859..5611
                     /locus_tag="ON430_001615"
     CDS             4859..5611
                     /locus_tag="ON430_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3219534.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5633..6787)
                     /locus_tag="ON430_001616"
     CDS             complement(5633..6787)
                     /locus_tag="ON430_001616"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCR3219535.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            6972..9413
                     /locus_tag="ON430_001617"
     CDS             6972..9413
                     /locus_tag="ON430_001617"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCR3219536.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9743..10447)
                     /locus_tag="ON430_001618"
     CDS             complement(9743..10447)
                     /locus_tag="ON430_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219537.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10634..10879)
                     /locus_tag="ON430_001619"
     CDS             complement(10634..10879)
                     /locus_tag="ON430_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219538.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(10896..11747)
                     /locus_tag="ON430_001620"
     CDS             complement(10896..11747)
                     /locus_tag="ON430_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3219539.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            11913..12917
                     /locus_tag="ON430_001621"
     CDS             11913..12917
                     /locus_tag="ON430_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCR3219540.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4176 a         2311 c         2712 g         3838 t
ORIGIN      
        1 aatcttagta tatttttctc tacttgaata aaaaaattat aggattggag aaaataatcg
       61 agaaatttgt tgcttgaata atagccagtt agacatttcc ttgactgttt cagttgtcat
      121 ggatgaacaa tgtgtctcat cttctttgaa ctgagtagca agttcttcta aaaactcagg
      181 atcgtaaatg acagcacttg tttcaaaatt caatcggtag ctacgaatat cttgatttgc
      241 tgaacctacc atgcagattt catcatccat tatcaatgtt tttgcatgga ggaagccgtt
      301 ctcatagaca aggatttgta cattttcctt gatcagctgc cgagcataat attgtgttgc
      361 tcgataaata aaaggatgat ccggtttgtt gggaatcatg attttcacat ctacaccaga
      421 ggcagttgcg atttttaaag cagcaatgac actttcatca ggaacaaggt aaggtgtctg
      481 tatccaaaca cgttttttag cagaagtaat caatttgata aatgaaagct tgatttgttc
      541 cctttcgtta ttcggtccgc ttgaaacaag ctggatggat gtatcagcaa gcttagattc
      601 atctcgatct gcttttttaa agaaataatt caaatgatag ccgacctttt tttcttcagg
      661 taccgagacg ttccagtctg ttaagaagcg aagctggagc aaagaagaag cggcaccgaa
      721 aatccgtata tgcgtatcgc gccaatagcc gaattttttt gttgtattta catattgatt
      781 ggcaatattg aaaccaccgg tataactaat ctttccatcg atcacaacga tttttcggtg
      841 atcatggtaa ttcaatcgga aacgaagtaa tgccctttgt gaggtaacaa atgtatggac
      901 atgtccacca tttttgatta gacgattgaa atcttttgct tttgtgccat gagagccaaa
      961 tgcatcatat aatattcgaa cttccacgcc ttctgcggct ttttcttcta aaacatgtaa
     1021 gacttgctgg ccgatattat ctgtcacaaa tgcgtaatat tcgatatgga tcgaatgttt
     1081 ggcttttttt agatcttgaa gcagtgaatc caatttctct tgtccgtctg tgagaagagt
     1141 gacagaattc attcttgtca gcggcatacg atttaatgaa gagaagaagt caacaaattg
     1201 ctgtttgtct ttatcgccca tgtcaggatc gtaatgttca atggtatctc ctctaaaaga
     1261 ctgaaagttt tctaattctt tcaagtcact ttgtcggaga tagaattttt tcttatccgt
     1321 taatccacgt ccgaaaaata aatataatac aaagcccacc ccaggaaggg caaacaatac
     1381 taagagccat gcccaaatgg ctgctacatc tcggggtttg atcaaaatag ccaccaaagc
     1441 aataagtgca tttaatagat aaagggcggt tataatacta gataccacaa aaatgttcct
     1501 ccttacatac aataacatta gtatagcatg atagaagaaa gaagcctacc aaggtgaata
     1561 tacttttcac tcctttcatg aaaatgtagg taaatcgggc taaagtagtt gctttttgta
     1621 ggttaataag gtaggatatt agtacagttg aatatctcct taggggagta accggcaacg
     1681 tttgttgcga taatatcaac agtaagttaa ccaaatggtt ttctggtatt gtatgaaatg
     1741 gtgagaccta agacaaatgc gtatttgtct taggtctctt ttttttcaga caggactagg
     1801 gccaagatca aaaaatgggg gatgaaattt ggaaaacaag gaacaagcac aacaagcggt
     1861 ctataaagaa acgatcgact cgttgatgca gcaaacgcag agctcaaccg atggattgag
     1921 caccacgcaa gcgaaggaaa aattagaaag agatggcttg aaccagctaa aagaaaagcc
     1981 gaagaagcca acttggaaat tgttcttaga aactttcaaa gatgcaatgg ttatcgtatt
     2041 gctgattgtc gcactgattc agatggtcat gggtgactac atcgaattct tagttatttt
     2101 tgcagtattg atgttgaatt ctataatcag tgttgtccaa acaaaaaaag cagaaggttc
     2161 tttagaagcg ttgaaaaacc tttcagcgcc cgatgctcga gtgattcgtg atggtaagga
     2221 aaaaacgatt ccggcaaacg aacttgtggt aggagatatc gtgttgttag acgcaggcga
     2281 ttatgtacct gctgatggac gtttactaga tgcgggatca ttaaaagtag atgaaggtat
     2341 gctgacagga gagtcgactt ctgcagaaaa agaagtaacg gatatcacaa gtacggtacc
     2401 aatcggagat cgtacaaata tggtattcag tggaacgatc gtaacttacg gacgcggtaa
     2461 attcttagtg acttcaactg ggaataatac ggagatcgga caagttgctg gccttttgga
     2521 atcaacaact gagcaagtca ctccgttaca acgaggtctg gataaattca gtaaaaaact
     2581 aagtttggct attttggcat tgtctttatt gattttaggt atccaactgt ttcgtattta
     2641 tcttggagaa ggtacaggag atatgactgc agatatcgtc agcgcagtga tgtttgccgt
     2701 agccgtagcc gtagctgcta ttccagaagc attacagtct atcgtaacaa tcgtgttatc
     2761 tttgggaacg aataaaatgg caaaacgtca tgccattatt cggaagcttc cagcagttga
     2821 aacacttggt tcgacaagta tcatctgtac agataagact ggtacattga cacaaaacaa
     2881 aatgacagtt gttgactact ttttaggtaa tggcgatact ggggatttca caaatgaccc
     2941 ttcaaaatgg acagctgacg aacgtcgctt gatcgaaatc agtgttttag cgaatgatgc
     3001 atcgatcagt gaagacggta ctaaattggg cgaccctacg gaagtagcgt ttatcgactt
     3061 tagtgaaaaa ttaaaccagc cttatcagga aatccgaaac aactacccaa gacaagcaga
     3121 acttcctttt gactcagatc gaaaattaat gtctactgga cacacaattg ataacaaaaa
     3181 ttacttgttt gttaaaggcg gaccagatat cgtcttccaa cgcagtaaaa aagtattgct
     3241 aaatggtgaa gtcgttccga tgacggatga attgatgaaa aaattccaag agcaaaatga
     3301 agctttttca acacgcgcat tacgtgtact agcttttgct taccgtccac ttgataattt
     3361 tgatttatca tttgaagatg aagacgattt gatccttgtt ggtttgcttg ctatgatcga
     3421 tcctccacgt gctgaagtga ctcaggcggt aacagaagca agaagtgcag gaatcaaaac
     3481 aatcatgatt actggtgacc acaaaacaac agctgttgct atcgcaaaac aaatcgggat
     3541 ttttgaagaa ggaaacctgg ctttgaccgg gacggaacta gatgaattga cagaagatca
     3601 actaaacgaa gttttagaaa aagtcactgt ttatgcacgt gtttcacctg agaataaaat
     3661 tcgtatcgtt cgtgcttggc aaaataaagg tcatatctca gctatgacag gtgatggtgt
     3721 aaatgacgca ccagcattga aacaagcaga catcggaatt gctatgggaa cgggtacaga
     3781 tgtggcaaaa gatgcagcag cgatggtatt gacggatgat aactttgctt ctattgtcag
     3841 cgcagtagaa gtaggacgta atgtgtttga taatatcaag aaagctattt cttatctatt
     3901 tgcaggaaat ttaggtgcga tcattgcgat catctttgcc ttagttgctg actggtccaa
     3961 tccatttaca gcattacaac tgttgttcat caatttagtc aatgattcac tgccagcaat
     4021 tgcattaggt acagaaaaag ctgaacctgc tacgatgaac cgcccgccaa gagatccaaa
     4081 ttcaggaatc tttacggaca aaacattgat ttctgtaacg tatcgtggtg tgctgattgg
     4141 gattgctgtt atctttgctc aatggcttgg catgcagcat tcacctgaat tcggtgtagc
     4201 catgtcattt tcaacattga tttggtgtcg gacattgcaa acattcccag cacgttcaaa
     4261 cacacaaaca gctatccaag cagggttctt ctctaacaga agtgtcttac tagctgtagt
     4321 tgtatgtagc gcgctttact gcttaacaat gatcccagga ctgaaagaag tcttctctat
     4381 cccatcagca tttggattga cagaagtctt tacatgtatg ggaatcgcac ttgtgacaat
     4441 tgttttgatg gaaatcacta aattgatcat tgtacgagta caaggaaata aataaaacga
     4501 atcaacgagg ttgtgagcct gccgtttaat tctgagtcac aaggtgcaat ttcaacaaac
     4561 agcttctcat gtttgttgaa tattgtgact tgtgaccgaa gagttggcag gagaacaccg
     4621 tttaaggaag aagttgtaaa atcagcattt tgagctaata ctgagggtca tctgattgga
     4681 cactgtttat tcggaaagag ggactttgac acgactttgg tcacagtccc tttttttatt
     4741 tatgatagac ggaaagccaa tcattttatt attcatcaaa agagatagaa tagataaaag
     4801 aggagaatga tttattccga aaagtgaaaa catataacaa aaaattagag gaaatcaaat
     4861 gaatgaaaaa gaatgggatg aatttgctga agaatattat gaaatccagc aagaatcgca
     4921 agtaactatt gctgaagatg taaaaaatta tctgaaaacg gagaaagtcc tgcctgctga
     4981 aacagcagca gacgttgctg gaggatctgg cagatttctt tccttagcaa aagaaattaa
     5041 tcgctatgat ttgatcgatt tttcttcagg aatgctagaa aatgcaaaaa aagaagcaga
     5101 aaaaaatgag ttgccaaatg tttcctttat caaacagtcg ttagaagaat ttatccaatc
     5161 aaatgagaca tatgaaatgg tttttacagc agcaaatcca gcattggtac atcttattca
     5221 attagaaaaa ttgatgaaaa agtcaagcaa atggtgcgtg ataatcagag tagtggaatc
     5281 aatcgatgat gttttttatc ctatcgaaaa gcaactagga atgatagaag aagatcctaa
     5341 tacatcgcca gttgtaatgg atcaatttga aacatactta attgatgaag gattcaaagt
     5401 gaagaaaaaa gattttactt attttacaca ggaatcaatt actgaagaat tgatgaagct
     5461 atactatgac gcgtacgcaa aagatcctgt tgtgaccagg tacattcgca aactattcag
     5521 tgaaaaacga gaaatcctta gtacgactaa gctgacctat agactgctcc tttggtccgc
     5581 agataaaaaa agaacctttc acgactcgtg aagggttctt tttatgattt tattatattt
     5641 tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct gaagtatatt
     5701 ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat tcgtcaacag
     5761 tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga tattcttgat
     5821 agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg tagctgaata
     5881 gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa gttagcccca
     5941 ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg ctttcttcat
     6001 agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg ctttgcaatg
     6061 ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa tcttcgagtc
     6121 ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg atatacgtag
     6181 aattagataa aggaccatca acaacaggaa attcgctata atctggacgc cagaaatcat
     6241 agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc gggttttgag
     6301 tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt gccaagccgt
     6361 aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt ttgacatatt
     6421 ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact tcaaaagaac
     6481 gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct tttgaatgat
     6541 caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct ttttctgtta
     6601 cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca cgattgactg
     6661 ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca cggctttctg
     6721 ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga tcaatcccta
     6781 ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag gtatcataaa
     6841 aagattaaaa gaaatataaa aaagagaata tttaaggatt gttaaaattt aatcttagaa
     6901 aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata tttttgtagg
     6961 aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg ataggaaagt
     7021 atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg actaaaggat
     7081 tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt ggaaatgtcc
     7141 tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc ggtctccctg
     7201 tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa gcagtgatct
     7261 tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc ggaacagaga
     7321 gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa tatggtgaac
     7381 caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca catatgggat
     7441 taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag gatcgctatg
     7501 ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt tctgaggaga
     7561 tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca gttcgtggaa
     7621 atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa ggaactgtaa
     7681 cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt gcatccaaag
     7741 aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta gcggaggtcg
     7801 gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa ttgacagata
     7861 gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca tttgcagctt
     7921 cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat atatatggag
     7981 gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg actacattag
     8041 cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt attggtggag
     8101 gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag gatgtgcaaa
     8161 aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata gaaaaaaatg
     8221 tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa gagctctccg
     8281 atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc gcgcagaatt
     8341 ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca tctgtcattg
     8401 cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca cctcagcggc
     8461 tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg atcaatgcag
     8521 ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct tcaatcgtga
     8581 aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt ttccatgcat
     8641 acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac atgatcaact
     8701 cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa gagattctat
     8761 tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt gaaatcccat
     8821 tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa atccaacagg
     8881 caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa gggatcatga
     8941 atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt agcgcttcga
     9001 tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg cagatcaaag
     9061 gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta ggcggagctt
     9121 ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat tcagccaagg
     9181 aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct ttgcgtgcat
     9241 tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca ttagcgatct
     9301 caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt gaagcagaaa
     9361 agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa taaaaaagca
     9421 atatctttag aagaataaag caagatttac agttatttta tttactgcag atcttgcttt
     9481 tttctctatc agaaaatatg tttattatta gaatttttta tagttaaggt taggcatctt
     9541 aagaaatcag gcaactccaa gatatcttca ggtagaagag aagtcgccta acattgtagg
     9601 atcataggtg atttgttgga caggtcaagc tgatatttga gcctgatgtt agaaggaatt
     9661 tctttttact tgctgtagaa attattatat gactaaaaag gctgggattt taaatcccag
     9721 ccttttatga caaatagaat ttttatcgat cagtaatcgc tgtcgctgca aattgttcga
     9781 tcaaaggaat caaagtcaat ttgactttgt atttccgttc gagtgctttt tcgataacga
     9841 actcattttc agtagtcata gaaatagttg ctaatttacg aggttcatca gcattttttt
     9901 cattaataac tacttcatgt tcattttgtt ctgtgtaaaa tttaggatac tcagcaacgg
     9961 cttttgaaaa atcagcaaaa cttaaagcgg cattcgtatc agttgagcgt gtattttcag
    10021 ttgtgtccgc ttttggtttt tccgtttttt cttctggcac actgactgtt tcttctgtaa
    10081 ctggtgtcgc aacagcttgc gcagttggtt catcgttttt caatcgttga tcattccagt
    10141 aagacacgat ttcaataaga agattgtcac ggaatcgttc gaaaatcccg ttttctcctg
    10201 aacgaattct ctttaataca gtattgaagt tgtcatcgat tggcacgttg atccgtgtat
    10261 cttcatttgt tttcccagct aatccgatcg ttaagatttg acggtttttg ggatcaagca
    10321 tgacttgtaa cggcaaacca taacgtcgca agctctcttc ttctagagaa cggaataatt
    10381 gtcctcttac accatccaat gaaggacgga attgatttaa taatttttgt ttctgttttt
    10441 tcttcatatg ttctctcctt cgctcatacc ctttcgatta taagaaaaaa acagccaaat
    10501 gaaaaggagt aatgcaaaaa aacttgcgta tacgcaaatt acaacagaaa aatttgcgta
    10561 ttttcccgtt ttttgaaaga aaaataaaaa atggaacgga ttatgtatat atacataatc
    10621 cgttccattc tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa
    10681 tgacttcatg ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt
    10741 cttcgatgat ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat
    10801 cgatcaaaaa gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca
    10861 agcgttcgat tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct
    10921 tttgctttgc cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat
    10981 tcaaaatcaa ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga
    11041 ggaaggaatt cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt
    11101 gcttcagcga tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta
    11161 tatgcttcga ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg
    11221 cctggtgcaa ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta
    11281 tctagatggt ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac
    11341 agttcttcaa atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca
    11401 acagggtttg gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat
    11461 gattcttcaa aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt
    11521 gtcaagaata tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct
    11581 tcattcttgt atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta
    11641 acagaggaaa cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg
    11701 atttcgtaac catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct
    11761 tttttattcg ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt
    11821 ctactcttga aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt
    11881 ttccgtaaac taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc
    11941 tgcatattat tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga
    12001 caaaagaata tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa
    12061 caaaaactgg acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag
    12121 aaaattttgt ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa
    12181 gtgaagaagc acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt
    12241 acttatcttt attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg
    12301 aagcagggat cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa
    12361 aagcagatga agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa
    12421 aatatgagtt ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc
    12481 ctgcaccttc tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa
    12541 aaaaatttga gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata
    12601 cgatagaaga aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa
    12661 acatcacggc aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag
    12721 cacctcctgt aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca
    12781 agctatcaaa cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga
    12841 ttgaatttgt gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag
    12901 tattgaatcg tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta
    12961 aatgaattgt gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtgaga
    13021 caaaagtaaa gaacact
//
LOCUS       DAJSMI010000072        12829 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJSMI010000072 DAJSMI010000000
VERSION     DAJSMI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.072"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            79..2244
                     /gene="acm"
                     /locus_tag="ON430_001622"
     CDS             79..2244
                     /gene="acm"
                     /locus_tag="ON430_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCR3219541.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2516..3001)
                     /locus_tag="ON430_001623"
     CDS             complement(2516..3001)
                     /locus_tag="ON430_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCR3219542.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3091..3436
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3481..4122
                     /locus_tag="ON430_001624"
     CDS             3481..4122
                     /locus_tag="ON430_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3219543.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4141..4776
                     /locus_tag="ON430_001625"
     CDS             4141..4776
                     /locus_tag="ON430_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3219544.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4794..5609
                     /locus_tag="ON430_001626"
     CDS             4794..5609
                     /locus_tag="ON430_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3219545.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5928..7364
                     /locus_tag="ON430_001627"
     CDS             5928..7364
                     /locus_tag="ON430_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3219546.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7553..10000
                     /locus_tag="ON430_001628"
     CDS             7553..10000
                     /locus_tag="ON430_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR3219547.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10230..11297
                     /gene="rlmN"
                     /locus_tag="ON430_001629"
     CDS             10230..11297
                     /gene="rlmN"
                     /locus_tag="ON430_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR3219548.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11490..12593)
                     /locus_tag="ON430_001630"
     CDS             complement(11490..12593)
                     /locus_tag="ON430_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3219549.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4379 a         2155 c         2733 g         3562 t
ORIGIN      
        1 gggaggaatt gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa
       61 ataggggaga tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt
      121 attactaata taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc
      181 agcagtaatg tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata
      241 aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg
      301 atcaattggc caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta
      361 atgattgaaa ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg
      421 tttaatgaaa atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag
      481 gccagaaacg taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt
      541 gataaaacag ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt
      601 tattataaaa caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat
      661 attaacaata acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt
      721 ggacaaagat tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag
      781 gtttatagag gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt
      841 aatttttcag taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt
      901 aggaaaataa tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag
      961 aatcattcgg aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc
     1021 aatcatacag ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa
     1081 ttgaagattt tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa
     1141 ttaagacgag cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat
     1201 gaacagggag aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa
     1261 gaagcaccca attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata
     1321 aacgaaaatg atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac
     1381 atcacagcaa ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa
     1441 ttattcagag caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta
     1501 gacaatggaa taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac
     1561 gaatatacgt ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt
     1621 tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc
     1681 tcaggacaaa aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc
     1741 acagtaaatc tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat
     1801 gattggcgct atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat
     1861 actgtaacgg aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa
     1921 aacagttata caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg
     1981 aaaaattttc cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg
     2041 caaatatttg ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga
     2101 gctgtaccta aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa
     2161 aaatctagtt ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc
     2221 ttacaaaaat ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa
     2281 tcaaaagaat aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt
     2341 ttttcgtaga atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt
     2401 tcagcgttca tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt
     2461 gtgctaaata aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca
     2521 aattttcata aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat
     2581 ccataatcaa cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa
     2641 tagcagcatc gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag
     2701 tcactgtacc tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata
     2761 agcgcttttc ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca
     2821 agatgacaag atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg
     2881 gaccaggtag ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt
     2941 gaaaagagat acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca
     3001 tcgcgcatca ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa
     3061 taatcatgta aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt
     3121 aggtgttttt agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat
     3181 cttggagtcg attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg
     3241 gtcgttgcta gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg
     3301 gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa
     3361 cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt
     3421 ataatacgtc ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag
     3481 atggagtatt tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa
     3541 ttatttttat ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg
     3601 tctttgcatt ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga
     3661 acaccgctgt tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt
     3721 tttgatcgtt tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca
     3781 gagatctttc gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt
     3841 ttgcaattaa gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt
     3901 gttttacctt ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat
     3961 atattaggtt taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact
     4021 ttactgcctt tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc
     4081 gtaatgaaac aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat
     4141 atgattgaat tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg
     4201 aatctaatca ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa
     4261 accacgcttt tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta
     4321 gacggtgtac cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta
     4381 ttccaagact tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca
     4441 aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct
     4501 tcattaggga ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa
     4561 cagcgggtag cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa
     4621 ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg
     4681 aaagaagatg gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc
     4741 ggtgatcaat tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa
     4801 aaaccagttt tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca
     4861 aaaaaacttc agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt
     4921 tagacgatac atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg
     4981 atattgattt agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca
     5041 ttgactggtc aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg
     5101 gctattccaa aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa
     5161 acgatcaagt gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata
     5221 ataagagatt aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag
     5281 aagtattaaa agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag
     5341 cattgatcga tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag
     5401 actattattt gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg
     5461 agagtgagga ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca
     5521 atgaagccct agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt
     5581 ttggcgaaga tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca
     5641 cagtcccttt ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa
     5701 tggcaggctc acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc
     5761 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta
     5821 agcgactttt tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt
     5881 tattctaaaa gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag
     5941 aacttacctc atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag
     6001 aaggagcttg gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac
     6061 ctggaaagac atttaaagga acgaacggtg atttggcagt agaccactat catcgttata
     6121 aagaagatat tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt
     6181 ggacacggat ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg
     6241 agagattgat cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt
     6301 gggatcttcc acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg
     6361 aagatttcac tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact
     6421 ggattagttt gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc
     6481 cgccaggtgt gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa
     6541 atgcatctgt catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct
     6601 ttgcgtatac gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa
     6661 atgcagaaga cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga
     6721 ttgctgccat gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg
     6781 cagaattgct agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga
     6841 cgaatgcatt caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa
     6901 aaggttcatc agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg
     6961 tcgaacgaac aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc
     7021 gtatcacaag ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg
     7081 acaagttgac agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc
     7141 acgtcaaagc catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct
     7201 ggtcttatac agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat
     7261 atgttgatca agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt
     7321 actggtacca aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac
     7381 aagaaggtac ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga
     7441 aaaactgtaa aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta
     7501 gttcccttac ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca
     7561 aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca
     7621 gcaggaacaa atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg
     7681 ctttttaaaa ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg
     7741 aaatacttac caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa
     7801 gaatacagag ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt
     7861 cggtatctta ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa
     7921 tatcatgggg acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa
     7981 taacgttggt ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc
     8041 acagcctgga agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag
     8101 taatctggaa ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga
     8161 attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat
     8221 gaaacagcgc gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa
     8281 agaagctgca gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga
     8341 tgaatggcag gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag
     8401 ttttcaagaa aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca
     8461 gaccaataca gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga
     8521 atttgagatt gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct
     8581 tgattctata ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg
     8641 cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt
     8701 aggaccatcc gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat
     8761 tgtgaactta acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc
     8821 accgattcca ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt
     8881 ttcctttaga gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc
     8941 tttaggacgt gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt
     9001 actcgtggct ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag
     9061 tatattaatg cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt
     9121 tgaattgaat atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag
     9181 aaaagcagcg caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt
     9241 cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt
     9301 ggaaaacgga acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc
     9361 tgatttgatg atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat
     9421 ggctcgggca gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat
     9481 cacaggaatc atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac
     9541 agattctcgg acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat
     9601 gctattcttg ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga
     9661 ccacgaagta gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga
     9721 gaaaatgatg ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga
     9781 gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct
     9841 ccaaagaaga tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc
     9901 tcatggagtt gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga
     9961 agaaacatca gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt
    10021 gaaaaacgaa taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt
    10081 tttccatctt ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt
    10141 tctattcgat ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc
    10201 gaaactttga gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga
    10261 atgaagaatt aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag
    10321 tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga
    10381 aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg
    10441 tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga
    10501 ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag
    10561 gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga
    10621 ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc
    10681 ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg
    10741 acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca attggtgctc
    10801 ggcacattac agtttccact agtggattag cacacaaaat caaggaattc gctaataacg
    10861 gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga
    10921 tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt
    10981 tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata
    11041 gaccagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg
    11101 tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag
    11161 ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta
    11221 aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa
    11281 aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt
    11341 ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata
    11401 aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca
    11461 gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt
    11521 tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg
    11581 atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct
    11641 aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc
    11701 ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt
    11761 acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta
    11821 ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc
    11881 aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg
    11941 tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt
    12001 ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta
    12061 gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct
    12121 tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt
    12181 ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc
    12241 gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca
    12301 gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca
    12361 tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg
    12421 acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca
    12481 gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt
    12541 tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt
    12601 tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct
    12661 gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac
    12721 cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa
    12781 taatgaattt ttgttcctct tagtatttct accgattccc tttgctttt
//
LOCUS       DAJSMI010000073        12401 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJSMI010000073 DAJSMI010000000
VERSION     DAJSMI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.073"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            77..1960
                     /locus_tag="ON430_001631"
     CDS             77..1960
                     /locus_tag="ON430_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR3219550.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2035..2319)
                     /locus_tag="ON430_001632"
     CDS             complement(2035..2319)
                     /locus_tag="ON430_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3219551.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2309..3043)
                     /locus_tag="ON430_001633"
     CDS             complement(2309..3043)
                     /locus_tag="ON430_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCR3219552.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3172..3795
                     /locus_tag="ON430_001634"
     CDS             3172..3795
                     /locus_tag="ON430_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCR3219553.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3987..5108
                     /locus_tag="ON430_001635"
     CDS             3987..5108
                     /locus_tag="ON430_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCR3219554.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5115..8243
                     /locus_tag="ON430_001636"
     CDS             5115..8243
                     /locus_tag="ON430_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCR3219555.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8262..11456
                     /locus_tag="ON430_001637"
     CDS             8262..11456
                     /locus_tag="ON430_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCR3219556.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11602..12309)
                     /locus_tag="ON430_001638"
     CDS             complement(11602..12309)
                     /locus_tag="ON430_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3219557.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKGKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4225 a         2198 c         2624 g         3354 t
ORIGIN      
        1 acgattctac ttgctatttt cacttaatac ccgtatatta cgaatgaaaa aagtttatga
       61 aaagttggaa tattgaatgg aaaaacaaaa aaggttaaaa aacagccgct acatcactgg
      121 gtttgatggg ctccgttcat tagctgtggt gggtgttatc ttctatcacc ttctgccaac
      181 tagtatgaaa ggcggctatt tgggcgttcc gatttttttc gtcgtatctg gttatttgat
      241 cacggatctg ctgcggcagg aatgggaaca aaatggaaaa atcaatattt ggcaatttta
      301 tgtcagaaga atgaagagac tctatccggc acttgttttt atgcttgtga cagcttcggc
      361 ttatatcact ttgtttcagc gagggctgtt aaacaatctt cgaggagttg tggtcagcag
      421 cttactgtat gtcaacaact ggtggcaaat caataacggt ttatcttatt tcgatcgatt
      481 tgctaatgag tcaccgttta cgcatatctg gtctttagca gtagaaggtc aaaactattt
      541 ggtttggccg atcttgtttg tattgttgat ggtatttgtt cgtaaaagaa aatggatctt
      601 ttatactgtg ttaggtacat ccttcctctc cgccatcttg atgatgatct tgtatacacc
      661 aggaggcgat ccaacccgtg tttattacgg taccgacact cgtctgtttt ctatatggtt
      721 gggaagtgcg cttgcgtttg tttggccaag tacgcgttta aagaaaaaca tcccgaaaca
      781 agcgaaaaat attctgaata tcgctggagg aatctcgctt ttggcactga ttctttgctt
      841 cttcttttta gatgatcatt acaatttcat ctattacgga ggaatgctcc tagtgagtct
      901 gttatgcgta gtgctagttg cagtgacagc acaccccgga gcaagtttga atcgatggct
      961 gacaaatcca gtatttactt ggattggcaa acgaagctat ggcatttatc tgtatcagtt
     1021 tccagttatg attttttatg aagcaaaggt gactgatatt gccgatcatg ttttgttgca
     1081 tactttagtg gaaatcacat tgattctaat catcagtgaa ctttcttatc gatatgtgga
     1141 gcgtccgcta gcacgttttg attatggtcg tacattccaa gtgatgaaag gatggttcag
     1201 caaaccgatc atcagtaaga gcaaaccatg ggttgttcca ggcacattac ttgtagcaat
     1261 tgcactcgtt ggtttcgtta cggcaccgaa aaacgctgta acagccgatc aaaaacaact
     1321 gcaggcaaaa atcgctgaaa gtaaaaaatt agcagaagaa accagaaaca attcagaaaa
     1381 caccactagt tcggttgacc aagcgatcct tgataagtac gagttgtcca aggcacaagg
     1441 gaataaggca caaaaattag aactaacggc atttggtgat tcagttatgt tggatgctgc
     1501 aacggatcta caagaacttt atccaaaagt agtggtagat ggagatgtcg gacgacaatt
     1561 atatgccagt gtaccgtaca ttgaaaaatt aaaggaacaa gatttgttga aagacactgt
     1621 tctgatcgga ctaggaacga atggctcatt ctcggaagct cagtttgata gtgtgatgga
     1681 tgcaatcggt gatcgaaaag tatactgggt caatgtccga gtaccgacga agcgctggca
     1741 aaatgacgtg aatgcgatgc ttgaacagat gacgaaaaaa tacgacaata tgacgctgat
     1801 tgattggtac agctacagta atgaccatag tgactggttt tatgatgatc aagtccatcc
     1861 aaatcctgaa gggatgacac attacattca cttagtcagc gaagccttgc taggaggaag
     1921 ttcaagtacg aacagcagtg agacaaacgt gacagaatga tagctacaaa aaacttcagg
     1981 caaaaacatt tgtttttgcc tgaagttttt ttatatttac cgtgttttta acattcatat
     2041 agattcagga agcaaaacgg ttgagtgtgt ctgtaaataa cgttcttttt gttttcgggt
     2101 gagttttttg aatgcagctt ctgcttttgt tgcttcgctt cttgaagaaa atacttctgc
     2161 atggatcatc ttaacaggtc ttcttttttg aagacgagta tatttagcac cagtaccatt
     2221 gttatgttcc tctaatcgtc tagaaagatc ggttgtatat ccaccataaa atgtctgatc
     2281 tttacagaat aatacgtaaa aataatgttc atttgccata gagcatctca cgaatctctg
     2341 cagtgtattc tccatccata ttgtaagtga tcaatggtgg agcgactttg aatccgtcta
     2401 atttgccttc tttgattcct tcaactaaca aaatatttgc ctcttttcca ggttttgggt
     2461 aaacaaattg gatcctttta ggtgcaattc gatagtcttc catgacctga atgatttcta
     2521 agaaccgatc aggacgatgg accatagtaa agcgtccatt tgtttttagt agcttacttg
     2581 aaatatcgat cacttcttct aacgtcgtat gaatctcatg acgggcgatt gccaggtgtt
     2641 tattagggtt cgttttattt gttggcagcc ctttgaaata aggtggatta cagacgacta
     2701 agtcacaaga attatgtcga acgacagata agctgtcttt cagatcgatg gtgtggacag
     2761 tcatttgttc atttaaatgg ttaagcgcga tactacgtct tgccatgtct gctagacgag
     2821 gttgaagttc gatagcgtcg atagccgcct gggttcgttt acttaaaaaa agtccaactg
     2881 cgccatttcc tgcacaaagg tcaacaatct tccctttttt aggtacacgg gaaaagtgag
     2941 caagcagcac agcatctaaa gaaaaagaaa acactttgga actttggata atcttgatat
     3001 cttctgcgta taattggtcg attcgttcgt cttcgtgaag catggattcc ctccaaaatg
     3061 aaaaaaatag tcgtctgtat tatactattt ttattaaaaa gaaccaaggt ggcttgtcaa
     3121 acctctattt tttatgcata ctatgaccat aagaagggaa aggaagagac tatgttttat
     3181 cgatttatgc gcggggtagt tcgagtacta ttagccttag ttaatggaaa tgcccgctat
     3241 gagaataaag aagttttacc gcaagatgag aattacatac tagttgctcc ccatcgtact
     3301 tggtgggacc cattatatct ggcggtagct gcagcaccca aggaattcag ctttatggca
     3361 aaagaagaac tgttcaaaaa tccagttctg cgatttatat taactcgatc caatgctttt
     3421 ccagttaaaa gagaaaatcc aggaccaagc gtcattaaaa cacctgtaaa gattttgaaa
     3481 tcgacaaatt tgagtttgat catgtttccc agtgggacaa gacatgctac agaacttaaa
     3541 ggcggaatgg cattgatttc cagaatgtct aaagcgaaaa tcgtgccagc tgtatatcaa
     3601 ggtccgttga cacttgggga tctgttcaag cgtcagagag tgacagtgcg tttcggcgaa
     3661 ccaatcgact tatcagatat caagaaaacg gataaagaag gtatggcgga gatcgaacga
     3721 cgaacacaag cagcttttga caaattggac aaagaagtga accctgattt caaatatgaa
     3781 gtcaaaaaaa aataaaatga taagtttatg cgtgggcggt cttttgaatc tactgtcctg
     3841 ttcagttcag actttttcaa tgaaaacgag ggagagacgt caagtctgcc ctctttttgc
     3901 gttccaatat gtaaaataga agacaggtgt catgtaatgg atcaaagtgt tataataatt
     3961 agaaggacat tttttgaggt gattgaatgc gttttttaca tacagccgat tggcatatcg
     4021 gaaaaaaact acaaggttat gacttgattg aagaccagcg tcatgtacta tcttctatac
     4081 tagatatcgc gaaagaagaa aaagtagagg caatcgtgat tgccggagac ttatatgata
     4141 ggagtgtccc agctgttgat gccgtagaat tatacaatga attgatggtc gattggaatt
     4201 taaaggaaaa gatcccgatt tttgctatat caggaaatca cgatagcagt acgagacttt
     4261 cagcgggtac gccatggttc agttattcga actatttttt gcgaacacag ctagcagaag
     4321 catttgaacc agtcgagttt aaaaacgtac aatttttctt tcttccttac tttgaaccaa
     4381 ttgcagcgag attatatttt gaagatgaca atatccgaac gatcaagcaa gcggtagcca
     4441 aggtgataga taaaatgcag gagctatttg attcagaaaa aaaacaagta ttgattagcc
     4501 atttttttgt aatgggaagt gtaaaatctg attcggaaac gaaaatcgaa gtaggcgggt
     4561 tggattcgat ccctggtgaa ctacttacac cgtttgatta tgtagcacta ggacatttgc
     4621 ataatcaagc tgctttgaga caggataatg ctcgctatag cggttcccca ttgaaattct
     4681 ctttatctga gatcaaccaa aaaaaaggcg tatggatcgt tgacctgaca acaggcctgg
     4741 agttgaattt tagggagatc gaacccctgc atgatattga agaaataaca gcgagtttca
     4801 aagaattgct ggatcctgcc ttttatcagt ccgtcaatcg ggagaattac atctatatct
     4861 atctcactga tagaacgatc atcccgaata tgatgaacca gcttcggcaa atatatcctc
     4921 gtattttggg gattgaacgg gcaaacggtc gagcagaaca aacaaagcgc aaacgaaaag
     4981 tactagaaat gaaagatcct caaaaattag cagaagcatt ttttagtgaa atgacggaag
     5041 agcagctaac tgcaaaccaa gaaaaatggc tcggagaaac gttgcaagaa atcatggaaa
     5101 tggaatgatc aagaatgaaa ccgaaaaaat tacaacttaa aaattttggt ccttttatca
     5161 atgagaccgt agatttttca cgtttgacag aagcaccgct ttttttgatc agcgggaaga
     5221 caggagctgg caaaacgacg atttttgatg ggatcacctt cgcacttttt ggtgaaactt
     5281 ccggaagact tcgctctgga aaagagatgc gttctttgtt tgctacacca gacgaagaaa
     5341 caagcgtgac tttttctttt gaacatcagc agatgagcta tgaaatcgaa cgaaaaccag
     5401 aacaagtttt agcaaaacga aaaggaaacg gaacaaaaaa acaggcagcc aaagtaacgt
     5461 tgaccatttt tgatgggaaa ggaaaagaaa taaaacaaat cacaaaacgt acagaagcag
     5521 atcagttgat cagagaactg atgaatctcg atgcgaaaca gttttctcaa atcgttttac
     5581 taccccaagg agaattccgt aattttttgg tttcatcaag cagtgataaa gagactgttt
     5641 tacgtcattt attcggtact caatttttcc aacagttcaa tgagcgatta aaagaaaaag
     5701 caaaacaaca gcaacaaaaa ttggatcatt tagagcaaga gctgcatctt ctgcaaaaaa
     5761 ggtttgtttt agtcaaagaa gaggaaccag cagcagggtt tgaagccgtt ttagcccaat
     5821 ggacaaacca tcaggcaata ctcgaaaaac aaatcacgga agaaaaagaa gctctaacgg
     5881 aacttcagga aaaacaaaag caactagaaa attcctacta tcgttttgaa agcttgaaaa
     5941 aaagttatga agaaaaaacg atattaataa acaagcaaaa ggaattagct gctcaaaaag
     6001 acgaaataga ggaaaagaaa cgatggattg ggtattatga atttacgcaa cgattagtcg
     6061 aaccaatcca gcgcctacaa gaaataaaag ttgaaaaaat agaactgtca caaaaaagta
     6121 gtgagcaaac aaagaaacta gcgatgacta cgcagaatta tgaagagtgg caaaaaaatg
     6181 aagcaaaccg cgtacgccga caaacaaaaa tcaaagaagc tgctcaaaaa ctgcaaaaca
     6241 aacagatgat ggtgccaatt gtcgaatcct tacgtagtaa aaaacaagac gcagaacaat
     6301 taacaatttc attggataaa gaagaattga atgccgagaa gctgtatcaa aatctcgcta
     6361 tctatcagga aagaactcga attcttcaag aaaatttact ggatcaggaa aaactgcaag
     6421 aagatcgtct tgcttttgga cagttaaaat atctagatga aaagcagcaa gaaatagaaa
     6481 aacaaaaagc aaaagacact gggtcgctcg cacagataag agaagaactc aaaaaagcaa
     6541 ctgaagaaat aacaaaaaat agtcagctat tgaaagaaca gacagaacaa gcagaacagc
     6601 tgaaaagcca atgggcaaag cagcaaattg ctcgtcttca gctttttttg cgtccagggg
     6661 agccatgtct tgtttgtgga tcaacggaac accccataca acaattaaaa cacgaacagc
     6721 caaataagga agatatctta aaagtagaac aacaactggc agaagctgag tctgctgtag
     6781 aaagaactca gcaaatcatt gccaaaagtg aagcacatga aaataatctt caacaacaat
     6841 tatcagattt agaacaaaaa atcatggcaa cgaatataca attggctgaa caagaagaac
     6901 acttgcaaca ggctttgaaa aaggcatttc ctgagataac agaacaaaaa acaaaaacaa
     6961 ctcttgtagt aatcgaacaa tctttagata gacgaaaaag caagatggcc aatgcaagaa
     7021 tagagctgga agaaataaga caacaagcag aaaagctcga acaacaatta gctgtctaca
     7081 aacagcagca gacagaaaaa aataatcagc tccagcaact gcaaggagaa atcaaaagtt
     7141 tagaacaaca attgcaagat acggatagta actgtttaga agaggagata cgacgacttg
     7201 aaaaacagtt agaagaagac tcgaatcaac tagctgttga gcagaaagca ggagtgcaat
     7261 tgttgcaaga tcttcatacg cttcggacac agcttgatct tcaacatgaa caaatagaaa
     7321 aaatagatga aaaaatagaa aaggaacaag aaagaatagc taaaaagtta gaaagccagt
     7381 cgtatttttc aacaattgaa gaaatctgtg catttgcaca acagcaagca caatataaag
     7441 aaaaccaacg tacagttact tcttatcatg aagagcaatt agtgacagct gaccggctgc
     7501 gtcaattaga aaatatccag ctaactgaga cattcccaga tacagaagag atcagccaac
     7561 agttagaaga aataagacgt atgatcgaaa taaagcaaga aaagatatac ggtttacaag
     7621 aacaaaaaat tagtaatcaa aaaatctacg atgaatgtct ttcgatttat cacacaagcc
     7681 agacgaaatt agatgagctt acgcaattac agcagctttc gcaaacattc aacggagaaa
     7741 atcctaagaa gactagtttg gaacggtatg tgcttcaggt ttatcttcaa gaagtgttac
     7801 aagtagcaaa tgatcatctc caacgtttaa cgaaaaatcg ttatcaattt gagctagcgg
     7861 ataccattgg cagttatcga gggaaaaccg gattagaaat caatatctat gatgatcagg
     7921 caggtatgtc tcgaagtgcg cataccctgt ctggcggtga gagttttatt gcagcacttt
     7981 ctttagcatt agctcttgcc gatgtgatcc agacacaagc tggtggtgta gcaatcgatg
     8041 cattgtttat tgatgaagga tttgggtcat tagatgaaga agctttggaa atggcaatgg
     8101 aagctttaga aacgatcgaa agtgaaggaa gaatgatcgg catcattagt catgtaagag
     8161 aattgaaaga acgagtatta caacaaataa gaattgatac agaaggtagc ggacaaagca
     8221 aaatacgcta tcaacttgga taaaaaggga gattggtaac tatgaaatgg agtattccag
     8281 aaaaagtcat tgcacgtgga cgtcaatact tgcaagaagg ccgagtgtta tcggttgtcc
     8341 ctgaccaaga agaaaacgtg tggcatgcag aagtcttggg cagtgaattg tatatggttg
     8401 atttggatgg tacagcaaaa gagatagatt actgccaatg tccctattgg gaggaacata
     8461 aatattgtaa gcatacagtt gctgtggaac tttatttacg gtcgaaaaat ctatctcgta
     8521 tcatgaaaaa agaccagcca atgctagaac cggtaaaagc aacaagtgag ggagaaatca
     8581 ttgcaaaagg tttttctcgt ctaaaaacac aaaacaatcc tcaagcggtg caacctttga
     8641 ttattgaata ccaagtagaa acgattgaga ctaatcagta tcacccagag ctttctattt
     8701 tggcacttta cttgcgtatc ggttcattgg catcaccgaa aaaaacatat atcgtaaaaa
     8761 acatctatga ttttttacaa gcttacacag aaaaagaaac ttacactgtt aataaacagt
     8821 accagtttcg tctagataaa cgggcgtttc aaaaagaaga tcaaaaaata ctcacgcatc
     8881 ttgcaggaag tgctcagacg catcaattat taggtacgaa cggcttacaa gtcaaaggaa
     8941 aactagataa aagatatctg cttctcccag tagaacaagc ccgcttcctg ttagagcaga
     9001 tgaatcagac tacacgtttg catctgcaga taaatgaaca aaacgatcaa ggaatacaat
     9061 tttccgatca aaacaaacct ttatcattca aagtggaaaa atttggagaa gattatttgc
     9121 tgcaagcatt gaatgatttt gattttttct ttatgcatta tcagtgggga gttatccaag
     9181 ggacatttta ttctctgacg aaatatcaac aaacggttta tcaaacttgg aaacaattga
     9241 ttcgccgatt ggagaaacca gaagtagtgt tcaagaaaaa agaattaccg tctttgttta
     9301 aagagattat ccctctgctg tcagagatcg gcgacgtagc tattgcccct gcagtagaag
     9361 aggaagtagc agatatcccc gtcgaattta ttttcttttt ccgaaaagca aaaggaaaaa
     9421 tccaagctcg agtcgatttc gtctatggcg aagtcatcta ttcgactgac ccgaagcatg
     9481 aggtctcgac agaacaggct tttcaggtgt tacgtgacac agtagaagag aaacgagtac
     9541 ttgatctatt cagaatgtat cgctaccaga aaaatgaaac tggttatgaa cgtccattgc
     9601 caagcgggga agaactatat gcctttttcc gcacagaact atctgtcttc cgtcagtttg
     9661 gtgaagtcag acttggaaga aaattgcgag agttgtattt agatgcgcat aaacatcaac
     9721 cgaaaatcga agtaaccgag accggttcat ggctcgatat tcgattcgat gtgacaggga
     9781 tagaggagca agaaatcgat catgtcctgc aaagcttgct gagaaatgat gctttttata
     9841 cattagaaaa tggtcaggtt ctttcttttg attcagaaga atttcaacaa acaagccaag
     9901 tgttgcaaca attacgggaa tcgatacgta cagaagaagg aacgatccat gtacctaaaa
     9961 atcaaggatt gatcattcaa aatcaattgg aaaaaagcaa tgctactttt agtgaatcgt
    10021 tccaaacgat ggtacaagat ctcattcatc cagaaagata ccaggcacag cttcccaaag
    10081 gattgaatgc aacgatgaga gattatcaaa agcaaggttt tcgatggttg aagatgttag
    10141 gtcattatca gtttggcggt attttagccg atgaaatggg acttgggaaa acactacaga
    10201 caattgcctt tttactttcg gaaaaagaag aacggaaaag cttctctgct ttgatcgtag
    10261 cgcctgcgag cttaatttac aactggcagg cagaggtgag aaaattcgcc ccttctttaa
    10321 gtatccaagt aatcaatggg aataaaaaag aacgtgaaga attactggct aaagacacag
    10381 atatccgagt gacttcttat gccagcttaa gacaggatct agctaattat cagtcgcaaa
    10441 aaattgatta tttgattctt gatgaggcac aaatggtcaa aaatagcagt acgaaaactg
    10501 cacaagcatt gagagaatta gctgtacctc agcggtttgc actgagtggg acaccaatcg
    10561 agaataattt agaagaattg tggtcattgt ttgcaacgat tatgccagga tttttcccga
    10621 ctaaaacaaa attcagggaa cttgccgcag aagaaattgc gcaaatgatt cgaccgttta
    10681 ttttacggcg agataaacaa actgtgttga aagacttgcc ggaaaaaaca gagatgaatc
    10741 tatattctgc acttacagaa gaacaaaaaa ctgtttattt agcttacttg cgtcaaatgc
    10801 gtgaagaaat cacatcaatg gattctgaag cattcaagaa aaaccggatc gggatacttg
    10861 ctggtctgac aagactgcga caaatttgct gcgatcctcg tctgtttatt gaagattacc
    10921 aaggcggttc cggcaagctc gaacaagtaa aagatctact ggtagctgcc aaagagaaca
    10981 aacgaagagt tttgctgttt tctcaattta cagggatgct gactatcttg caagaagagt
    11041 tagccgagct agggatctcg acattttatt taagaggcag tacgaaacca caagatcggt
    11101 taagtatggt agacgcattt aatgctggcg aaaaagatgt ttttctgatt tccttaaagg
    11161 ctgggggaac tggattgaat ctcacgggag cagatacggt catcttgtat gacctttggt
    11221 ggaatcctgc cattgaagaa caagctgctg gacgtgcaca tcggatcgga caaaaaaatg
    11281 tggtagaagt gtggcgtatg atctctgaag gaacgattga ggaacgcatg gattcactcc
    11341 agcaagaaaa acgcgaactg ttccaaaaag tgatccaagg taatgaagaa cagttgacga
    11401 aactgactga agaagacatc cgcatgattt taagtgtagg agaaatcgag gagtaagaga
    11461 ctcgaaaaag cgttcagtaa tgggtaagaa agcctttcta aagaagcgat tgtttatttg
    11521 atttaaaaaa tagcaaggag gctgggattc cattttatcc cagcttcctt gctatcattt
    11581 ttttatttct atttttaaag gttatacaaa gattgtcttc attgtctcta acagctgatc
    11641 aaaagaagaa atctcgatat cgcaaattgg agtctgttca attttgcgtt ctcgatggtt
    11701 gaaccaaagc gcttgccagt tagcactttt tgcacctaga acatcattgt cgtaattatc
    11761 gccaacgtat aaggtctctt ctggaagcat atgaaattct ttctcagcta gctgaaaaat
    11821 ctctttttct ggtttttgga aacctgttgc ttgagaaatg agcatattgc gggaaggaat
    11881 ccaattgttc aattgaagtt gcttcagttt ttttgtctga tggtcagtcg gcccatttgt
    11941 gataattcct aatgggacat tctttccctt caagaaatcg agggtttttt tgacctcctc
    12001 atgcaagcag atattatcaa gttcttcctc gtaaattttt tgaaataaga ggccatcttc
    12061 ttccgtaata tgagggtagt ctaaatcttt tagtgattgg ctgatccgat gtgctcgcat
    12121 atattctagt gtccaatctc ctgccattac ttttgggaag ttttcatcac tatgatggcg
    12181 gaaccggata tatagtggat gcatatctgc atctgaaaca agcggaacga ctcttttgac
    12241 cgcgtttcga aatggctgct gctgatcata catcgtatca tcaacatcaa acacaactga
    12301 tttaatcaaa attttacacg cctttcggtt ttcattgatt ttttcacaaa tccttgacta
    12361 ttgtaacaga aaaaattgtc taaaaaagct gataaataaa g
//
LOCUS       DAJSMI010000074        12309 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJSMI010000074 DAJSMI010000000
VERSION     DAJSMI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.074"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            27..3116
                     /locus_tag="ON430_001639"
     CDS             27..3116
                     /locus_tag="ON430_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR3219558.1"
                     /translation="MSLFYLKEKLEARIEELGKYRFSKIVELSDWLVVEDETKKAKYP
                     PKDFSSAATFSVGDTWKGRDFYLWVKKEIEMPDVDDLYLYLNFGHTGGGYNSGFESLM
                     FINGKPYQGVDSNHKEVKIADEFRGKKVEVALKLWSGLEGGGPEQIQVHQFKQAELSQ
                     MMVPVDELFYYADAINKTVKELGQDNPIQYKLSHVLNHAINQINWIEPGSQTFYTSVA
                     KAVSELSEGIAAIPKDEMITVTAVGHTHIDVAWLWRLKHTREKAARSFSTVLRLMEEY
                     PEYIFLQTQPQLYKYIKEDYPEIYAKIKERVAEGRWEADGSMWLEADCNIPSGESLTR
                     QILHGAKFIKEEFNQDIHYLWLPDVFGYSWALPQILTKSGIDTFMTTKISWNQFNRMP
                     HDTFKWKGMDGSEVLTHFITTPEPGKENSWESNWYYTYNGELEPETVLGVYNAYRDKN
                     INDELLISYGFGDGGGGVNRDMLEKRRKLDKIPGLPKVQTGKAVDYFDRLQETFKNTD
                     EYVHTWDGELYLEYHRGTYTSQAYVKKANRQSELLLRQLEILFSLMEMKGQEAYPKVE
                     IFNMWETVLRNQFHDIIPGSSIKEVYQDHRIEMEGLFKEAQSLLAPLQSVGDSQTVCN
                     FANWQRSILAELPFLLDNQVYVDEAGEVIETYANGEVLYGLISNVPALGQKMIHVTEK
                     QEMAQIPTVAVVDSNQIETDFYLIQWNKSGQLTSVFDKELKREVLSGTGNVLQLFEDK
                     PMNFDAWDIDIYYQEKFQELAAQEIIVKPANALYQCVEFIYRFGESTIKQEMRLYPHT
                     RRIDFVTDIDWQERQQLLKAKFDVAVRATYATYDIQYGNVRRANNWNTSWEMAKFETV
                     AHQWMDMSQRDFGVSILNDSKYGCDVKDQTMRLSLLKGAIHPDPTADMGQHHFTYSLY
                     PHRGDFIEGKTIQEAWEINEKPLVLNGSIGTLPQITTTQDATIVIDTIKKAEDGNGWI
                     IRAHEYAGTDQTVDLTIEGLTWWRETDLLERNIGEVKDKQPSLTFAPYEIKTIRFGK"
     gene            3085..4452
                     /locus_tag="ON430_001640"
     CDS             3085..4452
                     /locus_tag="ON430_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCR3219559.1"
                     /translation="MKLKQFVLGNEGRALEITAEFCQKLKSNYEGTPKYKLAELGVVR
                     NGVVESAENVSAKSQNPFVFSIEVPTLKIPQQYHSGRCWLFAIVNLLNQQISQKLEIA
                     EFELSQNYLYFYDKLEKANFFYKNIIETADLELGSRELDFLLSKPQEDGGQWDMLVSL
                     IEKYGIVPSYSMPETENSRSSADMNKYLNAKLRLDAKKLRQMVAEDCSQKDIDEVTSQ
                     MLAEVYRMLAICIGAPPEKIDMKYRDDAGNYHAGLNLTPKEFANKYLLMNLKDYIPII
                     HAPSKNKVYNQTYTVAMMGNIIEGQPIKYLNVDMATLKALTIKQLKDHEAVWFGSDIG
                     QYTDRKLGIMATDIYAVDNLFDVDFTSNKQDRLEYCQSLLTHAMVISGVDLQNDQPTI
                     WKVQNSWGEEIGYKGFFMMTDAWLEEYVYQVVINKKYLTKEQLAKYQEEPVILPPWDP
                     MGSLA"
     gene            4527..5345
                     /gene="iolB"
                     /locus_tag="ON430_001641"
     CDS             4527..5345
                     /gene="iolB"
                     /locus_tag="ON430_001641"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HCR3219560.1"
                     /translation="MNLLQKPILFPVNEKVQLIQYITGENSVLEHVELSVYDVEAGGE
                     VSYHEAALESCVVVLSGQVSVAILNGETYPKLGTRTSVFDKIPTHSVYIGKNRSYKVT
                     AETKARILIAASKTDQSLPDRLIKPEDVLLESRGAYQNKRYVQTILSDQDTISDKLLV
                     VEVYTEGGNFSSYPPHKHDQNRLPEESFLEETYYHEINPPQGFVFQRIYSDDRLLDQT
                     MSCSNQDVVIVPKGYHPVGVPDGYTSYYLNIMAGPTKMWRFYDDPDHEWIKNRS"
     gene            5395..6372
                     /gene="iolC"
                     /locus_tag="ON430_001642"
     CDS             5395..6372
                     /gene="iolC"
                     /locus_tag="ON430_001642"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003573134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HCR3219561.1"
                     /translation="MKKFAITALGRACIDLNSVEINQPMEETKTFKKYVGGSPANIAI
                     GLAKLGETVGFIGKLSNDQHGRFIKDFMSKAGVDSSQMIFDQEGHKSGLAFTEIKSPS
                     ECSILMYREDVADLYLTPDEVSEEYIEASEMLLISGTALAQSPSREAALLAVEYAHKH
                     DVKVIFELDYRPYTWGSTAETAVYYTLLAQKAHIVIGTRDEFDVLENSVGGNNQQTVE
                     RLFQYTPELILIKHGVDGSFAYEKDGTITRGYAYKAQVLKTFGAGDSYAAAFLFALMQ
                     GKMIENALQYASASSALVVSRHSSSEAMPSVSEIEAVIAEQSWGEKVHA"
     gene            6365..8281
                     /gene="iolD"
                     /locus_tag="ON430_001643"
     CDS             6365..8281
                     /gene="iolD"
                     /locus_tag="ON430_001643"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HCR3219562.1"
                     /translation="MPKTLRLTTAQALVKYLNQQFLIVDDQKLPFVKGIFHIFGHGNV
                     LGLGQALSQNPGHLEIYQGKNEQGMGQAAVSFAKEKLRRQIFAVTTSVGPGAANLVTV
                     AGTALANNLPVLLLPGDTFASRQPDPVLQQVEHFHGQGITTNDALKPLSRYWDRIERP
                     EQLMSALTRAFEVLTDPVTAGPVTICLPQDVEGEAYDYPEDFFNEKVHYFDRRLATDR
                     ELSEAVKLIERSLKPVIIVGGGAKYSEAGSALKRFSEKHKIPLVETQAGKSTVVADFP
                     LNFGGVGVTGGAPANRLVKEADLVIGLGTRYTDFTTASKTAFDKDQTTFLNINVSRMQ
                     SYKMNGVSVVADAKTALLQIESELDDLYQTQYGDLLTSVTRDWQNERERLANLNYHDA
                     DFEMEIKNHFGAEKLANYAQQLTTELTQTAALIKINQSLPLDSIVVGAAGSLPGDMQR
                     LWKAKTVNSYNMEYGYSTMGYEVAGAYGAKIAHPDKEVFAFVGDGSFHMLHSELLSSI
                     QYGKKINVLLFDNSGYGCINNLQMGNGMPSFGTELTDYHEEIMTVDFAKIAEAYGAVA
                     YNVGNMSELEKSLAEIAESDRSTLIHIKVLPKTMTDGYDSWWNTGIAEVTADGSTEKV
                     LAEKEEKLAGAFQY"
     gene            8303..9457
                     /locus_tag="ON430_001644"
     CDS             8303..9457
                     /locus_tag="ON430_001644"
                     /inference="COORDINATES: protein motif:HMM:NF012678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR3219563.1"
                     /translation="MYQLTLPQKIIYGDQAIKETGKIAGNLGLKTLIITDKLIVSLNL
                     LEGVLASLKEENLTWKIFDGVDREPTDQIVAEALAFYQQEDYDSIIAVGGGSCIDTAK
                     AVAVLINNSGSYAEFASGQLIAQNPLPLIAIPTTAGTGSEVTDVTVITDTQKDIKYMM
                     KQAAFLSYAAIVDPQMSRTSPASVTAATGLDALCHALESYISKKAQPITRLFSLSAIK
                     LIMENLPIAYSEPENLIARENLAQAAMEAGIAFSNASVTLIHGMSRPVGALYHIPHGI
                     SNAILLEAVIDYTKPVIISELAGIAKYLNPEKEGTDQELAECFFQELQDLIEKLAVPN
                     YASFGIEKAAFIASLDKMANDAIASGSPNNNPLVPTIEEIKMIYLASWQE"
     gene            9480..9911
                     /locus_tag="ON430_001645"
     CDS             9480..9911
                     /locus_tag="ON430_001645"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3219564.1"
                     /translation="MEIKTDLNSEDSSQLAEFLLMVLDQQQEHFFPDYQTIDKSVALA
                     AYSGTELVGGLLGKISDQSLNVSLLAIDPKHQKDGLGSQLIEEVEKIAAKNQVINLLL
                     STRSYQALAFYQKNNFEIYGELKDMPFRGVDTYYLVKRLMA"
     gene            9949..10814
                     /locus_tag="ON430_001646"
                     /pseudo
     CDS             9949..10814
                     /locus_tag="ON430_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
     gene            10877..11836
                     /locus_tag="ON430_001647"
     CDS             10877..11836
                     /locus_tag="ON430_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3219565.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(11823..12077)
                     /locus_tag="ON430_001648"
     CDS             complement(11823..12077)
                     /locus_tag="ON430_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219566.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
BASE COUNT         3924 a         2163 c         2684 g         3538 t
ORIGIN      
        1 aaatgaaaag ggtggaggaa tagttcatga gtttgtttta cttgaaagaa aaattagaag
       61 caagaatcga agagttagga aaatatcgct tttcaaaaat agtagagttg tctgattggc
      121 ttgttgtaga agatgagacc aaaaaagcaa aatatccacc caaagatttt tcaagtgctg
      181 ctactttttc agtaggggat acttggaaag gccgcgattt ttatttgtgg gttaaaaaag
      241 aaatcgagat gccagatgtt gacgatttat atttgtattt gaattttggt cataccggtg
      301 gtggttacaa ctccggcttt gaatcattga tgttcattaa tggaaaaccc tatcaaggcg
      361 tggattccaa tcataaggaa gttaaaatcg ccgatgaatt ccgtggtaaa aaagtcgagg
      421 tagcattgaa gctttggtca ggtttggaag gtggcggacc ggaacaaatc caagtccatc
      481 aatttaagca agcggagcta tctcaaatga tggtgccagt tgatgaacta ttttattacg
      541 ctgatgctat caataagacc gtgaaagaat taggtcaaga taacccgatt caatacaagt
      601 tgtcccatgt tttaaatcat gctatcaacc agattaattg gatcgaacct ggaagccaaa
      661 cattttatac ttcggtagcg aaggctgtat ccgaattatc agaggggatt gccgcaatcc
      721 ctaaagatga aatgatcact gtgacagcag tcgggcatac ccatattgac gtagcgtggt
      781 tatggcgatt aaaacatacc cgcgaaaaag ctgcccgtag cttctcaact gttttacgtt
      841 tgatggagga gtatccggag tatattttct tacaaacaca accgcaatta tacaaatata
      901 tcaaagaaga ctatccagaa atttatgcaa aaatcaaaga acgagtagct gaaggtcgtt
      961 gggaagcgga tggaagcatg tggttggagg cggattgtaa cattccttct ggtgaatctt
     1021 tgacccgcca gattttacat ggtgcgaaat ttattaaaga agaattcaat caagacattc
     1081 actatttgtg gttgccggac gtttttggct attcttgggc attgcctcag attttgacta
     1141 aaagtggcat tgatactttt atgacgacaa agattagttg gaatcagttt aatcggatgc
     1201 ctcatgatac cttcaaatgg aaagggatgg atggctcaga agtcttgacc cattttatta
     1261 ccacgccaga accaggcaaa gagaattcat gggaatccaa ttggtactat acctataatg
     1321 gtgagttaga acctgaaact gttttaggcg tttacaatgc ttatcgagat aaaaacatta
     1381 atgatgagtt gttgatttct tatggatttg gcgatggcgg aggcggcgtt aatcgtgata
     1441 tgttggaaaa acggcggaaa ttagacaaaa ttcctggctt accgaaagtt caaaccggta
     1501 aagcagtgga ttacttcgat cggctgcaag agacctttaa aaatactgat gaatatgttc
     1561 atacctggga tggcgaattg tatttagaat atcatcgagg cacctatacc tctcaagctt
     1621 atgtcaaaaa agcgaaccgc caaagtgaat tattgttgcg ccagttagaa atcctctttt
     1681 cattaatgga aatgaaggga caagaggcct atcccaaagt tgaaatcttt aatatgtggg
     1741 aaacggtttt gcgtaatcaa ttccacgata ttatcccggg atcttcaatt aaagaggttt
     1801 atcaagatca tcggattgaa atggaaggac tttttaaaga agcccaaagt ttattagcac
     1861 cattgcagtc agtaggtgac agccaaacag tttgtaactt tgcaaactgg cagcgttcaa
     1921 ttttagctga attaccattt cttttggata atcaggttta tgttgatgaa gctggtgagg
     1981 taattgaaac atacgctaat ggtgaagtgc tttatggatt aatttcaaat gttccagctc
     2041 ttggtcaaaa aatgattcat gtcactgaga agcaggaaat ggcgcagatt cctacagtag
     2101 ctgttgtgga ttcaaatcaa attgagacag acttttattt gatccagtgg aataaaagcg
     2161 gacaattgac ttcggtattc gataaggagt taaaacgaga agttttaagc ggaaccggga
     2221 atgtcctgca attatttgaa gataaaccga tgaattttga tgcttgggat atcgatattt
     2281 attatcaaga aaaattccaa gaactagcag cacaagaaat tatcgtcaaa cccgctaatg
     2341 ctttatatca atgtgtcgaa tttatttatc gttttggtga atcaaccatt aaacaagaga
     2401 tgagattata tcctcatact cgtcgcattg attttgtgac agatattgat tggcaagagc
     2461 ggcaacagct gctaaaagct aaattcgatg ttgctgtcag agcaacttac gcaacttatg
     2521 atattcagta cgggaatgtt cgtcgtgcca ataactggaa tacgagctgg gaaatggcta
     2581 aattcgagac tgtcgcccat cagtggatgg atatgtcaca acgtgatttt ggggtcagca
     2641 tcttaaatga ttccaaatat ggttgcgacg ttaaggacca aacaatgcgt ttgagtttgt
     2701 tgaaaggagc aattcatcct gatccaacgg cggatatggg ccaacaccat tttacctaca
     2761 gtctgtatcc acatcgtggc gactttattg aaggaaagac cattcaagaa gcttgggaaa
     2821 tcaatgaaaa accgcttgtt ttaaatggta gtattggcac tttaccgcaa attaccacaa
     2881 ctcaggatgc gactatcgta attgatacga ttaaaaaagc cgaagatggt aatggttgga
     2941 ttatccgggc ccatgaatat gccgggactg atcagacggt tgacctgaca attgaaggtt
     3001 taacttggtg gcgtgagacg gacctgttgg aaagaaatat tggcgaagta aaagataaac
     3061 aaccgagttt gacatttgca ccatatgaaa ttaaaacaat tcgttttggg aaatgaggga
     3121 agagcattgg aaataactgc agaattttgt caaaagttaa aaagtaacta cgaaggaacc
     3181 cccaaatata aactagcaga attaggtgtt gttcgaaatg gtgttgttga atcggcagaa
     3241 aatgtctcag ctaaaagtca gaatcccttt gtgttttcaa tcgaagtacc gaccttaaaa
     3301 attcctcagc aatatcattc gggacgctgt tggctgtttg cgattgtcaa tttactgaat
     3361 cagcaaatca gtcagaagtt ggaaatcgct gaatttgaac tttcgcaaaa ttatctatat
     3421 ttttatgaca agttagagaa agccaacttt ttctataaga atattattga gacggctgat
     3481 ttagagttgg gcagtcgtga attagatttt ctactatcta agccccaaga ggatggtggt
     3541 caatgggaca tgctagtttc attgattgaa aagtatggga ttgttccatc ttattcgatg
     3601 ccggaaaccg aaaatagtcg cagttcagct gacatgaaca agtatctaaa tgccaagttg
     3661 cgacttgatg caaaaaaact gcgtcaaatg gtggctgagg attgttcaca aaaagacatt
     3721 gatgaagtga cttcccaaat gctcgcggaa gtttaccgaa tgttggcaat ctgtatcggg
     3781 gctccgccgg aaaagattga tatgaagtat cgtgatgatg ctggaaatta tcacgctgga
     3841 ttaaatctaa caccaaaaga atttgccaat aagtatttgt taatgaattt aaaggattat
     3901 attccgatca ttcatgcacc ttccaaaaac aaagtgtaca accagaccta cacagtagcg
     3961 atgatgggaa atattatcga aggacaaccg attaaatatc ttaatgtaga tatggcaacg
     4021 ttaaaagcat tgacaatcaa gcaactgaaa gatcatgaag ccgtctggtt tggtagtgat
     4081 atcggccaat atactgaccg aaagctaggg attatggcca cagacatcta tgctgttgat
     4141 aatttattcg atgtcgattt tacttcaaat aaacaggacc gtttagaata ctgtcaaagt
     4201 cttttgactc atgccatggt aatcagcgga gtagatttgc aaaatgatca gccgacgatt
     4261 tggaaagtcc agaatagctg gggcgaagaa atcggttata aaggcttttt catgatgacc
     4321 gatgcttggc tagaggaata tgtctatcag gtggtgatta acaaaaaata tttaacaaaa
     4381 gagcaactgg caaagtatca agaggaacca gttattttac caccctggga tccgatgggt
     4441 tctttagcat aataaaaaaa ccgcggctag tcataacgag atgattgttt gtgaccggcc
     4501 cccgatttta gtaaagaggg aaaataatga atcttttaca aaaaccaatt ttatttccag
     4561 tgaacgaaaa agttcagttg attcaatata taaccggaga gaatagcgtc cttgagcatg
     4621 ttgaattaag tgtttacgat gtcgaagctg gcggtgaggt tagttaccat gaagctgctc
     4681 ttgaaagttg cgttgttgtt ctttccggcc aagtgtccgt agcgatttta aatggagaaa
     4741 cctaccctaa gcttggaacg agaacttcag tttttgataa aataccgact cacagtgttt
     4801 atattggtaa aaatcgttca tacaaagtta cggcagaaac gaaagcgcgt attttgattg
     4861 ctgcttctaa aacggatcaa agtctaccag atagattgat caagcctgag gatgtcttat
     4921 tggaatcacg gggtgcttac caaaataaac gctatgtgca gacgatcttg tctgatcaag
     4981 atacaatcag cgataaactt ttagtcgtgg aagtttacac agaaggtggt aatttttcta
     5041 gctatccacc tcataaacac gatcaaaacc gcctaccgga agagtctttc ttagaagaaa
     5101 cttattatca tgaaatcaat ccaccacaag ggtttgtttt tcaacggatt tattccgatg
     5161 atcgtctttt agatcaaacc atgtcctgtt ccaatcaaga cgtggtaatc gttccaaaag
     5221 gctatcatcc tgttggcgta ccagatgggt acacttctta ttatctaaat atcatggccg
     5281 gtccaacgaa aatgtggcgt ttctatgatg atcctgatca cgaatggatc aagaaccgat
     5341 cgtaagagtt tttcagaaaa ataaaacgtg tcatgagaga ggaagaaaat taaaatgaag
     5401 aaatttgcta ttacagcttt agggagagcc tgtattgatt tgaactcagt agaaattaac
     5461 caaccaatgg aagaaacaaa aacatttaaa aaatatgttg ggggatctcc ggcaaatatt
     5521 gcgattggtt tggccaagtt aggggaaaca gtaggtttca tcggaaaact ttctaatgat
     5581 cagcacggtc gatttattaa agacttcatg tcaaaagcgg gtgttgattc aagtcagatg
     5641 atttttgatc aggaaggaca taaatccgga ctggctttta ccgaaattaa gagtccatca
     5701 gaatgcagta ttttgatgta tcgtgaagat gtggctgact tatatctaac ccctgatgaa
     5761 gtttcggaag agtacattga agccagcgaa atgctattga tttcaggaac agctttagcc
     5821 caaagtccgt caagagaagc agcgttattg gcggtggaat atgcccacaa acatgatgta
     5881 aaagtgattt ttgaattgga ttatcgtcct tatacgtggg gatcaactgc tgaaacagcc
     5941 gtttactaca ctttgctagc acaaaaagcc cacattgtga ttggtacccg cgatgaattt
     6001 gatgtgttag aaaatagtgt tgggggtaac aaccaacaaa ctgttgaaag acttttccaa
     6061 tatacgcctg aactgatcct gattaaacac ggggtcgatg gctcatttgc ttatgaaaaa
     6121 gatggcacga ttacgcgagg atatgcctat aaagcacagg ttttgaaaac ctttggggca
     6181 ggtgattctt atgcggccgc tttcttgttt gctttgatgc aaggaaaaat gattgagaat
     6241 gctttacagt acgctagtgc ctcgtcagcg ctagtagtta gtcgtcatag ttcttcggaa
     6301 gctatgccaa gtgtgtccga aattgaggca gtcattgcag aacaaagctg gggggagaaa
     6361 gttcatgcct aaaacgctac gcttaaccac ggcccaggca ttggttaagt atttgaatca
     6421 acagtttttg attgtagacg atcaaaagct accctttgtg aaaggtattt tccatatctt
     6481 tggccacgga aatgtcttag gattgggaca agctttgtca caaaatcctg gtcatttgga
     6541 aatctatcaa ggaaaaaatg aacagggaat gggccaggcg gctgtttcct ttgcaaaaga
     6601 aaaactgcgc cgtcaaattt ttgcagtaac gacttcagtg ggaccgggtg ctgcgaatct
     6661 tgttacagta gctggcactg ccttggctaa taatttgcca gtattattat tgccgggaga
     6721 tacttttgca agccgtcaac cagatccagt cctgcagcaa gtcgaacatt ttcacggtca
     6781 aggaatcacg acaaatgatg cattgaagcc actttctcgt tattgggatc ggattgagcg
     6841 cccagaacaa ttgatgagcg cattaacccg ggcgtttgaa gttttgactg atcctgtgac
     6901 cgcgggtccc gttacaattt gcttacccca agatgtcgaa ggggaggctt acgattaccc
     6961 agaggatttt ttcaatgaaa aagttcatta ttttgatcgc cgcctagcta cagatcgtga
     7021 attatcagag gcggttaagt taattgaaag aagtctgaaa ccagtgatta tcgtgggtgg
     7081 cggggcgaag tattcagaag ccggatcagc tcttaaacgt ttttcggaaa agcataagat
     7141 tcctttagtg gaaacccagg caggaaaatc aacggtggtt gctgattttc cattgaattt
     7201 tggtggtgtc ggtgtgaccg gtggtgcacc tgccaatcgt cttgtgaaag aagctgatct
     7261 agtgattggg ttgggaacac gctacaccga ttttacgaca gcctcaaaaa cggcttttga
     7321 taaggatcaa acgacttttt taaatatcaa tgtttcgcgg atgcaaagct acaaaatgaa
     7381 cggtgtttca gtggtagctg atgccaaaac ggccctattg caaattgaat ctgagcttga
     7441 tgatttatat caaacgcaat atggtgacct gttaactagt gttactaggg attggcagaa
     7501 tgaacgggag cgtctggcaa atttgaacta ccatgatgct gattttgaga tggagatcaa
     7561 aaatcatttt ggcgcagaaa agttagccaa ttatgcgcaa caactaacaa cagaattgac
     7621 tcaaaccgcg gctttaatca aaatcaatca aagtttgccg ctggatagta tcgtggttgg
     7681 ggcggctggc tctttgcccg gtgatatgca acgtctgtgg aaagcgaaga cagttaactc
     7741 ttataatatg gagtatggct attctacgat gggatatgag gtagcgggtg cttatggtgc
     7801 caagattgcg catccagata aagaagtttt tgcttttgtt ggagatggta gttttcatat
     7861 gttgcattcg gagctacttt ccagtattca atatgggaaa aaaatcaatg tcttactttt
     7921 tgataactca ggctatggat gtattaataa tttacaaatg ggcaatggga tgccatcttt
     7981 tggaacggaa ctgacagatt atcatgagga gatcatgacg gttgattttg caaaaatagc
     8041 cgaagcttat ggggcggttg cttataatgt cggtaatatg tctgaacttg agaaatctct
     8101 agctgaaata gctgagtctg accggagtac attaatccat attaaagttt tacctaaaac
     8161 tatgacggat ggatacgatt catggtggaa taccgggatt gctgaagtta ctgcagatgg
     8221 ttctactgaa aaagttttag ctgaaaaaga agaaaaacta gcaggggcct ttcaatactg
     8281 agaagcccag aaaggtgtca aaatgtatca actaactttg ccgcaaaaaa tcatttacgg
     8341 cgatcaagca atcaaggaaa caggaaaaat cgctggtaac cttggtttga aaactttgat
     8401 tattacagat aaactaatcg tctctttgaa ccttttggaa ggtgtgcttg ccagcctaaa
     8461 agaggaaaat ctgacctgga aaatttttga tggcgttgac cgcgagccaa cggaccagat
     8521 tgtagctgaa gcattagctt tttaccaaca ggaagattat gattcgatta ttgcggtggg
     8581 cggtggcagc tgtatcgaca ctgctaaagc tgtggctgtg ttaatcaata atagtgggtc
     8641 ctatgctgaa tttgctagtg gccaactgat tgcccaaaat ccactgcctc taattgcaat
     8701 acctacgact gccgggactg gctccgaagt gacagatgtg accgtaatca cagatacgca
     8761 aaaagatatt aaatatatga tgaaacaggc tgctttttta tcctatgcag cgattgttga
     8821 tccacaaatg agtcgtacaa gtccagcatc agttacagca gcaaccggac tagatgcatt
     8881 atgtcatgct ttagaaagtt atatttctaa aaaagcccag cctattacaa ggctcttttc
     8941 actttcagca ataaagttga ttatggaaaa tttaccgatt gcctatagcg agccggaaaa
     9001 cttaatagcc cgtgaaaact tggcgcaggc agcaatggaa gcgggaattg ccttttcaaa
     9061 tgcttcggtt accttgattc atgggatgtc aagacctgtt ggcgctctgt atcatatccc
     9121 tcatggcatt tccaatgcga ttttattaga ggcggtgatt gattacacaa aacctgtaat
     9181 catctcggag ttggctggta ttgcgaaata tcttaatcct gaaaaagaag gtacggatca
     9241 ggagttggca gagtgcttct ttcaagaatt gcaggacttg attgagaaat tagctgttcc
     9301 caattacgca agttttggta ttgaaaaggc agcttttatt gcatctttgg ataaaatggc
     9361 aaatgatgca attgccagtg gtagccccaa taacaatcct ttggtaccaa caattgagga
     9421 aataaaaatg atttatttag ctagttggca agaatagtca aagaaaagta ggtagaaaaa
     9481 tggagataaa aacagatctt aattcggaag attctagtca gttggctgag tttcttttaa
     9541 tggtcttgga tcaacagcag gaacattttt tccctgatta tcaaacaatc gataaatctg
     9601 tggccttggc tgcttattca ggaactgaat tggtaggcgg tctgctaggg aaaatcagcg
     9661 atcaaagctt gaacgtttcg ttattagcca ttgacccgaa acatcaaaag gatggtctag
     9721 gaagtcagtt gatcgaagaa gtcgaaaaaa ttgcggcaaa aaaccaagtc attaatcttc
     9781 tactttcgac gagaagttat caagcgctgg cgttttacca aaaaaataat tttgaaatct
     9841 atggcgaact caaagacatg ccgtttcgtg gcgttgatac gtattactta gtcaaacgtt
     9901 tgatggcata acgtttaaag ttccaataaa gaataaagag gcacaaaaat gacctacaaa
     9961 gaaataccag tctctgttca acaatttatg gaaaaaatca cgactctttg tggtaatgac
    10021 cacccacaat gggcagcaaa cattaatgct gcctttgcca atactctttt aacaacggtt
    10081 aagcgacatg aagatggcac aacttttttg ttgactggcg atattcccgc aatgtggttg
    10141 cgggattcaa ctgcccaagt tcggccttat ttagtcatcg ccaaagataa tccagatttg
    10201 gcggatatga ttagtggttt agtaaaaaaa caattcttct atattaactt ggatccttat
    10261 gcaaatgctt ttaatgaaga ggccaacaat gcggggcatc aggatgacga aacacaaatg
    10321 aatccttggg tctgggaacg aaagtatgaa attgattctc tttgctatcc aatccagctg
    10381 gcttatttgt tatatcagaa tactggccgt acagatcaat ttagtgaaga ctttattagg
    10441 gggatcgaga aggtcctgac ggtttttgaa acggaacaga accatcaaga ctcaccctac
    10501 acttttcaac ggtttgatga tcgaccggaa gatacgttag tcaatcacgg acgggaaacg
    10561 acagttgcac caacgggtat gacttggtca ggttttcgtc caagtgatga tgcttgccat
    10621 tatggttatc ttgttccgtc aaatatgttt gcggtggtca tccttgatta tttagtagag
    10681 attttccaag atattttgaa ggagacgaca ctgattgacc gtattatcaa gttaaaaaag
    10741 cgatcaatga tggtatccaa aaatttggta agatttttaa taacgccgat tgtaaaatta
    10801 agctagacaa ctaaaaaagt catttgtgct acactcaaaa tagttccgtc gaaagaatta
    10861 taaggagtga gtacaaatga cctataccca tcttacacca actgaactcg taatgataga
    10921 agcttatttc aatcaatcac actctgtttc aaaagtagcc catctggtac aacgttctcg
    10981 acaaaccatt tataatgtct atcgttttct taaatctggt ggaagcgcaa taagctacta
    11041 tgaacaatat aagaaaaaca aacgtaactg cggaagacgt ccgattgtgt taccagatgg
    11101 gcaacaagaa tacattcaaa aaaaggtggc tcaagggtgg actccagatg tccttattgg
    11161 tcgagcagag tttcctattg cttgttcggt acgtacacta tatcggatgt ttaaagataa
    11221 aacctttgac cctcacgatt taccaatgaa agggaaaaga aaaccaaacg gacataaaga
    11281 aaaaagagga aaacaagctt ttcgccgttc gattcaggat agaacaactg attatataca
    11341 gttcaatcat gagtttggtc acctagaagg tgacaccatt gtcggtggaa aacataaaag
    11401 tgcggtgatc acgttagttg agcgattatc taaagtgatc atcaccttga aacccgctgg
    11461 taggcaagct atagatattg agaatagact gaatcaatgg tttcaatctg taccgaaaca
    11521 cttgtttaaa tccatcacat ttgattgtgg aaaagagttc tcgaattgga aaaagattag
    11581 taataccaat gatatttcga tttatttcgc tgatccagga acaccttctc aacgaggact
    11641 aaatgaacat tcgaatggct tattacgtaa agatggctta tataaagcta tggattttaa
    11701 taaggtatcc gaaaccttta tccaatcggt ggcatcaaaa cgaaatcata ttcctagaaa
    11761 atcattacac tatcgcacac cattggaagt atttttgagt tacgtaggta aagatgattt
    11821 gtctagctta atttgacaaa cgaaactttg taaaaatatt cgattgaata ttctatttgg
    11881 cacgactcac ttggcagtat atgtatgtca atatttttca tctgtttcat aggtatttca
    11941 aaatctaaac tattaaaaac atataattcc ctgtaattat ttacatctaa ctctactaaa
    12001 tttatctttg gaacaataga tcctccaccc attccaccat aataaaaaaa atcttgttct
    12061 ctatcaaaat agcacatatt aagctcagtt tgtttatcat cctcaataat tttataaatc
    12121 aaacctatat aatcatgaat gctggcaaaa atttttttta agtcaagttc taaatcttct
    12181 tcgaaaacac atttaatttc tcctactgat aattgatact ctatattaca tgacttaatt
    12241 agttttttag atcccccctc cagataggca tatgtttcaa taattatgtt ctttttattg
    12301 cttggcaaa
//
LOCUS       DAJSMI010000075        12278 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJSMI010000075 DAJSMI010000000
VERSION     DAJSMI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.075"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            140..460
                     /locus_tag="ON430_001649"
     CDS             140..460
                     /locus_tag="ON430_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219567.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ON430_001650"
     CDS             912..1112
                     /locus_tag="ON430_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3219568.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ON430_001651"
     CDS             1259..4048
                     /locus_tag="ON430_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3219569.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ON430_001652"
     CDS             4095..4622
                     /locus_tag="ON430_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCR3219570.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ON430_001653"
     CDS             4643..5833
                     /locus_tag="ON430_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3219571.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ON430_001654"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ON430_001654"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR3219572.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="ON430_001655"
     CDS             8224..8898
                     /locus_tag="ON430_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3219573.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="ON430_001656"
     CDS             8895..9236
                     /locus_tag="ON430_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219574.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="ON430_001657"
     CDS             9266..9625
                     /locus_tag="ON430_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR3219575.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="ON430_001658"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="ON430_001658"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR3219576.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         4077 a         2147 c         2659 g         3395 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttct
//
LOCUS       DAJSMI010000076        12188 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJSMI010000076 DAJSMI010000000
VERSION     DAJSMI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.076"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(150..314)
                     /locus_tag="ON430_001659"
     CDS             complement(150..314)
                     /locus_tag="ON430_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219577.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            609..1937
                     /locus_tag="ON430_001660"
     CDS             609..1937
                     /locus_tag="ON430_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCR3219578.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2139..2972
                     /locus_tag="ON430_001661"
     CDS             2139..2972
                     /locus_tag="ON430_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR3219579.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2988..3725
                     /locus_tag="ON430_001662"
     CDS             2988..3725
                     /locus_tag="ON430_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCR3219580.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3880..4848)
                     /locus_tag="ON430_001663"
     CDS             complement(3880..4848)
                     /locus_tag="ON430_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR3219581.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5080..5913
                     /locus_tag="ON430_001664"
     CDS             5080..5913
                     /locus_tag="ON430_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR3219582.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5947..6786
                     /locus_tag="ON430_001665"
     CDS             5947..6786
                     /locus_tag="ON430_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3219583.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6812..7372
                     /locus_tag="ON430_001666"
     CDS             6812..7372
                     /locus_tag="ON430_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3219584.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7571..9292
                     /locus_tag="ON430_001667"
     CDS             7571..9292
                     /locus_tag="ON430_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCR3219585.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9457..10599
                     /locus_tag="ON430_001668"
     CDS             9457..10599
                     /locus_tag="ON430_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3219586.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10615..11826
                     /gene="thiI"
                     /locus_tag="ON430_001669"
     CDS             10615..11826
                     /gene="thiI"
                     /locus_tag="ON430_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR3219587.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4153 a         2046 c         2531 g         3458 t
ORIGIN      
        1 ctactgccgg agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct
       61 ttttctttta ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt
      121 tacgaaagag tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg
      181 aaataaatca gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca
      241 ttcccaaagt tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca
      301 atcaggtttt tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg
      361 agtattttga aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag
      421 gccattttcg ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat
      481 gacatttttc ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga
      541 agagattgta tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg
      601 atgattggat gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag
      661 caaatatcaa acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg
      721 ctgaaaatgt ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg
      781 ggaaagtggc taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat
      841 tccgtcataa gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca
      901 cttttttctg ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag
      961 gaaaagaggc aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg
     1021 gcggccaatg ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg
     1081 tgatgccgga aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat
     1141 tactgcgtaa agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg
     1201 ctattgaaga aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat
     1261 taggaacacc tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc
     1321 ttgaccgagg actgacacca caaatttttt atgataaata catcggtgta aaattagatg
     1381 attatgttag cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag
     1441 tagagatgct aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg
     1501 aaactttcaa aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt
     1561 gtgatgtagg tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa
     1621 tggatgatct atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag
     1681 aaagtctgat gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa
     1741 caaaatggaa agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta
     1801 tgagtgatgc ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga
     1861 cgaaagagca attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa
     1921 tgggtgcttt agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc
     1981 tggcttaagc cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg
     2041 ctataattct aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat
     2101 gacgaagtag aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat
     2161 ctgatctgaa attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct
     2221 atcgtataaa aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag
     2281 gattttttga agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg
     2341 atacgtatat gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc
     2401 cgggaaaagg ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag
     2461 cgccacaatt tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt
     2521 ttgaagaaga ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg
     2581 cagaagaaac gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac
     2641 gtgtattgaa atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt
     2701 ggagtggtaa tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt
     2761 cttatgggaa tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg
     2821 aatttttaga tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc
     2881 cgatttacca gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt
     2941 cccaagtaga tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat
     3001 ataccaaaaa gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc
     3061 cttcgatgtt aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag
     3121 gcagtgaatt cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga
     3181 ttaatcgtct ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca
     3241 ataagttttt ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca
     3301 aaatagaatc tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg
     3361 aagagatcat tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa
     3421 ttgaaaagat tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata
     3481 gtaatcagca tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg
     3541 actttttgac tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt
     3601 acgggaatga gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa
     3661 aacatgaaat ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata
     3721 aataaaaaag aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa
     3781 aattgtttct caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca
     3841 cagcctctct ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat
     3901 caattcttca cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg
     3961 actattaata ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc
     4021 ataatcataa acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat
     4081 tttttggaca agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc
     4141 taaagcttcg attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc
     4201 ttgcatccca gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag
     4261 tcgctttgtt tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat
     4321 gggacccaat gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta
     4381 gcgtgcagaa tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc
     4441 acaacttgcg acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt
     4501 acttgaacgc attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag
     4561 aaaaaaagca gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc
     4621 tgatgcataa gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg
     4681 tggtgaggga agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat
     4741 ttctgcatct aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc
     4801 tttcatggcg atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc
     4861 cttacgaata attttgcata gccgttaaat cttattgtat actatataaa agagttttgc
     4921 tatcagtgaa gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg
     4981 gaaaagactc aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa
     5041 gaaagcagcc aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca
     5101 tcgtttaaag aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca
     5161 gggtttctac ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca
     5221 gttgagaatt ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa
     5281 ggaatcagcg gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg
     5341 cattttttag atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc
     5401 caattcccct catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga
     5461 aagtggtttg agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat
     5521 gaatttcttg aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc
     5581 gatgagccac agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac
     5641 tttgctcttt ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag
     5701 tggcgtaaaa aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat
     5761 gaagtcaaag tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct
     5821 ggtggtgatg cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat
     5881 ggactcaatc caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg
     5941 aagtctatga tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca
     6001 aaagcgttgg aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc
     6061 ggtgtagcaa cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta
     6121 gatatgtatg tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac
     6181 cacccttttg aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga
     6241 caaatcgttt ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa
     6301 tcgattttta tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg
     6361 aaaaaaatcc tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag
     6421 atattaaaag aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag
     6481 ttagaacatc gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc
     6541 atcccaaaag gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt
     6601 gaaatggaag aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt
     6661 gtaggaattg gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt
     6721 gtgactcaat caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt
     6781 tattagtcaa tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt
     6841 agaaacattc cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga
     6901 aaatgaagct catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc
     6961 tacttcacct aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga
     7021 taaagcagaa gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca
     7081 agtagatgca ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat
     7141 agtgcctgat cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga
     7201 tggaaaacct cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat
     7261 ggaatatgct ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat
     7321 tgctaagaag aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt
     7381 tcactatttt tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga
     7441 atgcatgaaa atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta
     7501 tttggattta tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga
     7561 ggaaccataa atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc
     7621 cattgtcgtc tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc
     7681 cttagaaaaa cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt
     7741 taaaaaaatg catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg
     7801 gaatgacata tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa
     7861 cctcaatgaa agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac
     7921 aatggaccaa atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga
     7981 aacggaagaa cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa
     8041 agaaaaagtc agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca
     8101 gatcaaagtg atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc
     8161 tgttgaagcc cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct
     8221 gatgaaaaaa attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga
     8281 agaaatcagt gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga
     8341 tcttgctgaa gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga
     8401 aaaaacagaa gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc
     8461 gttatatgat atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc
     8521 aacgattgca gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct
     8581 ggatcacaca tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt
     8641 ccaaaccgaa gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca
     8701 acacgaaatt ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga
     8761 agatatcgaa aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga
     8821 aaaagaagcg caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt
     8881 tgtagaaaaa caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc
     8941 tactgaccgt attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga
     9001 ggtcaatcgt ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca
     9061 tgatttagta gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg
     9121 tcatacacac gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga
     9181 atatcactat caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg
     9241 tgctttcaaa cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa
     9301 agttagtaaa ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata
     9361 tttgaaaatt gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt
     9421 ataatagaaa agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca
     9481 acaaccccaa tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt
     9541 gggaatccat caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca
     9601 agaaagcaga tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc
     9661 ggaacagaag gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg
     9721 aatcatatga tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta
     9781 aaagaattag gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc
     9841 gacgaattga aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat
     9901 aatgaagtag gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct
     9961 aaaattcatt tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg
    10021 ttgacagaaa gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga
    10081 actggtttca tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg
    10141 caagaaagaa atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa
    10201 gctttacgtt tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa
    10261 gtagctctaa tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact
    10321 gactatgcac cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc
    10381 catgccttag aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa
    10441 catatggcaa gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct
    10501 gttcgaatca gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt
    10561 ttcaagcagc tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa
    10621 tacagtgaaa ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc
    10681 tttatcatgc aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa
    10741 atccatgctg atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt
    10801 attccaaaac tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa
    10861 aaagatgtcg aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg
    10921 aaagaaacat ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat
    10981 gaattgaatc aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa
    11041 atgcgtcaac cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg
    11101 tatgaaacga tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt
    11161 atgctttctg gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg
    11221 gaaattgaag ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa
    11281 gcaaaagatc taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg
    11341 ccatttaccg agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca
    11401 attactcgtc gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta
    11461 gtcattttga atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg
    11521 gctatcaatg aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt
    11581 gaaatcattg aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa
    11641 gattgctgta cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc
    11701 catcaatatg aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg
    11761 aagatggaag agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg
    11821 ctataagagg ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa
    11881 tagctccatg tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat
    11941 ttatcaagaa gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt
    12001 tagagatcaa gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga
    12061 ggttgtgaca gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc
    12121 aaatttttgt gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc
    12181 agaggttg
//
LOCUS       DAJSMI010000077        12133 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJSMI010000077 DAJSMI010000000
VERSION     DAJSMI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.077"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..437)
                     /locus_tag="ON430_001670"
     CDS             complement(<1..437)
                     /locus_tag="ON430_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCR3219588.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIG"
     gene            complement(624..1274)
                     /locus_tag="ON430_001671"
     CDS             complement(624..1274)
                     /locus_tag="ON430_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219589.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1817..2752)
                     /gene="whiA"
                     /locus_tag="ON430_001672"
     CDS             complement(1817..2752)
                     /gene="whiA"
                     /locus_tag="ON430_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCR3219590.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2786..3784)
                     /locus_tag="ON430_001673"
     CDS             complement(2786..3784)
                     /locus_tag="ON430_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCR3219591.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3781..4665)
                     /gene="rapZ"
                     /locus_tag="ON430_001674"
     CDS             complement(3781..4665)
                     /gene="rapZ"
                     /locus_tag="ON430_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR3219592.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4800..5531)
                     /locus_tag="ON430_001675"
     CDS             complement(4800..5531)
                     /locus_tag="ON430_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCR3219593.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5535..6800)
                     /locus_tag="ON430_001676"
     CDS             complement(5535..6800)
                     /locus_tag="ON430_001676"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCR3219594.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7063..9879)
                     /gene="uvrA"
                     /locus_tag="ON430_001677"
     CDS             complement(7063..9879)
                     /gene="uvrA"
                     /locus_tag="ON430_001677"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR3219595.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(9889..11883)
                     /gene="uvrB"
                     /locus_tag="ON430_001678"
     CDS             complement(9889..11883)
                     /gene="uvrB"
                     /locus_tag="ON430_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCR3219596.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
BASE COUNT         3473 a         2686 c         1959 g         4015 t
ORIGIN      
        1 ccgattagtg ttaaaatatc tttattcttc ttgctaacat aaccctttat gcacaaaaga
       61 acagctatac ttaaagtaac aaataaataa cgtattccaa tagaaaaatc actataaata
      121 ctctctagca taggaccgac aattgaagaa gtcactactg caaatgctgg acctatccaa
      181 gcaacattag aatgttcttt ctctcttttc ctttgtaaat ctttgttgag tattaatcta
      241 tttatttctt ctgcaggcaa gttaattttc ataaccttat gggggattat ccaattaaaa
      301 atcggaaaaa ggtagcctcc ccaaaaagta tagtgatctg tgtcaatcag ataataacct
      361 gattttgatt ccagaataac atatctcaaa gccgaacgaa aatttttcat gaatatccat
      421 aatctcactg tttccacacc taattcctcc aattccaatt aaatggattt atagcatcac
      481 caattatgtt aactgcctga ccaacctcat tgataaaatt ggcaccacta tcccatgctt
      541 caacaatgct atatatctat caaacaaaaa atggaaaact aaatactcgc gcacataaat
      601 tttacattct gttttttata agactaataa taatcattga aaaaatttct tatcggtggc
      661 gcatattccc aattgattaa tgtgatccca tattctgcaa gtattttacg agcttcttct
      721 aagtcatagt gagaaaaatg cataaaacct gcaatacgat cacggactgt gagctgtggc
      781 ttgcttccac catcattcat aaaatatcca gcagagccac tacttaaagt gattgttaca
      841 tcttcacttt ctattattaa gaattcataa tgaaatgctt tcaactctcc actattattt
      901 aaaagtttta tactccaact tcgtctaggt tgagttgtca cattccctct aaaacttcct
      961 ataaattctg caatcaccgt atcttcaatc aaaattcttt cctcatctat ttcatattca
     1021 agactaaacg gaaattctcc atatgtaatc tctggcaatg gtggtgctgt agttctccaa
     1081 ataaatacaa taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct
     1141 gctcgaagta aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa
     1201 ataaaaagta ctgcaatcat aatgatagta aaagttgtaa aaaagcacat tttttgccct
     1261 ccctttataa acatcgattc tcaaattcta aaccaaatct tgatagttca agttcaggta
     1321 taacgtatac caaccataca tcccaattac tatttatgaa cttatgatta tcactgaata
     1381 aatcactcca tccccttgtt aaaacttcta cattaattac acctggttgt tttcgactag
     1441 ctaatccaat gaacttatca accatcctta tccaccaacc acgagaatct tgaccagaag
     1501 taagaaaaac agttctcaag gttgatcgag cagttccttc cagtttttaa ttaatctcca
     1561 gttatagata aatacttgat cctatctata tcaggacttc tatattaagc aaaagtttat
     1621 gtataaaact aaaatcttca ttcagttgtc tccccgcttt ttatacgatt gtattaaaaa
     1681 acaatagaag cctttcaaca ttatgttact ccagtaaagt tggaattata gtttgacgga
     1741 agaaaagcaa gtatagcttt taaaaaaaac tgatacattc gcatctcctg cttcatgtat
     1801 cagttttttc tttggattaa actgcttctt cacgcagttt ttccgcaaat tcatttattt
     1861 tgcgaattcg gtgattaatt cccgattttg atattgcacc tgagggaatc atttccccta
     1921 attctttcaa gctgacctct ggatgttgaa gccgaagttc tgcgatttcc tgaagttttt
     1981 caggaagggc atgtagaccg acctttgctt cgatcaattc gatgttttcg atttgtttag
     2041 aagccgcatc aatcgtcttg ttcagatttg ctgtctcaca attgactaga cgattgaccg
     2101 aatttctcat atcccgtacg attcgaacat cttcaaatcg gagcattgag ttggttgcac
     2161 caatcaatgt taagaaatca gcaatttttt ctgctccttt taaatacgaa atatagccat
     2221 tgcgccgttc aagcgttcga gcatgtagac cgtaataatt taacatatcg caaatgtctt
     2281 ggttatgctg ttcataaata gaaaatattt ccaaatgata acgactcgtt tccggattat
     2341 tgactgaacc tgaggccata aaagcaccac gtaagtaaga acgcattttt tgcgaatttc
     2401 ccataatctc gtccgaaacg tgtccattga acatcatacc atccatgata tcaagatcca
     2461 tcaacacatc ttttgtgcct tgcttcaacc ggacgatata gacattgttc ttttttaatt
     2521 tcattttttt acgaacgagt agctcacttc gtacctgata atgatctttc aaaagagaat
     2581 agatacgtct ggcaattgca gcattttctg tttggacgtt caaaataaac tgctggttca
     2641 caatacctaa agagccgttc attcgcagca aagcagctaa ttccgctttc gcatgttctc
     2701 gatgaacttc caaaccagtt agttctttct tcacatctgc agcaaatgac atcctattct
     2761 cctcctttct ccaacaataa attgcttaat attttgaacc gaaaactaaa cggaacaatt
     2821 cttctactac tttgtctcca tcgtggaaga caccaccatc tttcaattct aaaaagtcgg
     2881 tggaaatcac tcgacatcct tcatttctca atccgttgaa gtcgtgtgcg acttgtacta
     2941 gatactcatc atagatctct gggtccatat aatcttcagg cactttttcc gtattgacca
     3001 atactgtatc gataaaaggc tgattcaaat gcttgtgtaa aacacgtata tgatcggcat
     3061 ctgtgaaatg ttccgtttcc cctttttggg tcatgatatt acagatatag acggtttcag
     3121 cagttgtctt gagcattgct tcgccgattt ctggtatcac taaatttgga aggatactgg
     3181 tgaataaact tccgggtccc aaaacgacca tgtcggcgtc cataattgct gaaacgactt
     3241 ttcgagccgc tttggcttca cttttatcat ttgtgttgca gacatacaca cggtcaatgg
     3301 ttttacggtt gatcgcgatt ttagattctc ctactgcagt cgttccgtct ttaaaaacag
     3361 catgcaaggt caaaggagtt tctgaagaag gataaacatg tccatctaca tgcatcattt
     3421 ttgctaataa ttgaatcgcc tcataagtgc ttccccgcat ctcggaaact gcggcaatga
     3481 tcagattacc gatcgtatga ttggctaaaa actgatcttc ctttttgaaa cggtattgga
     3541 aaatatcttc atagaattgt ggcatatccg ataacgccac taagacattt ctcaagtcac
     3601 caggaggagc catagcgatc gattctctga ttgcaccgct gcttccacca tcatctgcaa
     3661 cagtcactac tgctgtgata tctgctcctt gatttcgtaa acttttcagg ataactggca
     3721 gaccagtgcc accgccgacc actacgatct ttggttttct tatccggtag gttttcattt
     3781 tcacgaccgg ttcaccgtct ctttcctttt atccttgtcc cgatgtgtca cattgacttt
     3841 atagtttttc cctaaagcat ttgccaaacg ttcagtcaat gccacagaac ggtgttgacc
     3901 gcctgtacag ccgattgcaa ctgtcaagct gctttttcct tctttaatgt atccaggcaa
     3961 tacagtttcc agaagatcca aaaatttatg gtagaatgat tgcgtttcat caaaggacat
     4021 cacataatca tagactggct gatcttttcc ggtcaatggt cttagttccg ggatataatg
     4081 agggttgggc aaaaaacgca catccatcac aatatccgaa tcgatcggca acccgtattt
     4141 aaaaccaaat gatacaaatt cgatccgaaa tccttggtct ccctttgatt tgaatgactc
     4201 attgattttt tcacgtaatt gtcgtggtgt caatgttgtc gtatcaatga tcacggaagc
     4261 ttgggctttc aagtcgtcta gtatcgcccg ttctttacgt atcccttcag tgatcaaacc
     4321 gtccatcgcc attggatgtg ctcgtcttgt ttctttgtag cgagccacta gttcttcgtc
     4381 tgaagcatct aaaaataaga tagaagtgtc gatgaagttc gtattttcga tttcgatcag
     4441 catgttttgg atttcttcaa agaaggaacg agaacgcaaa tccactccta aagccatttt
     4501 tgtgacttta cctgattctt tgatcaattc ccagaatttt ggaatcaatc gaggcggcat
     4561 attatcgata caaaaatagc ccatatcttc aaaactttgg atagctactg ttttccctgc
     4621 accactcata cctgttatga tgactaattg caaattatct gtcatattta cgaacccctc
     4681 tcacattgtg cataataaaa ttatatcatc ccgggcgtat cattaccagt tttctttttc
     4741 ttttacaaaa agatttggac caaatatctt ttaggatatt tgccccaaat ccaatttttt
     4801 tacttttccg atgctgtatc caatgcctcg cttctttcag ctaatgcacg ttcgatcgtt
     4861 cgatccgcta aaatagattg agcatcgatc actgggtaat gattatcttc agcaaattca
     4921 tgcatcaaag ataattctgt gcaacctagg atgacttttt cacagtttag gcgctctacc
     4981 gcttcctcta agatttcgta atagagctct tgattcaaat aatccgattc cttgatctca
     5041 tgatagatca ggtaattgat tttttcttga agcgcagtat caggaatcac tgtttcgaaa
     5101 ccaagatttt tgacttcgcg ttcatagatt ccagctttca tacttccttc tgttcctaga
     5161 atcgctactc tccctgtcgt atgctgacgg acaagttcat ttgctgcttc tcttggcata
     5221 tgcaagatag gaatatccgt tgccgcttgt aactcttcaa aaaagtagtg tgcggtgtta
     5281 caagtcaaca caatgaagtt tggtcgcaat aaattttgtt tctcaatatc atccagcaga
     5341 aacggcattg gattttcttc tgatcgatct aaaatataag ctgtacgatc aggaactgtt
     5401 gcatgattga ataagacata attcaaatat tcttgatctt ttgtagcttt cgttcggtgg
     5461 ttgatcaagc gaacaaaact ttctgtcgcc atcgtgccca ttccgcctaa aatactgaaa
     5521 aaattctcca tgaactagcc ttctttcaca tgaaaatact ttttgaatct tggaatatag
     5581 ttatggaaca tatatttcat caacaaccag cgcttgattg atcggtcttt ttcataaaat
     5641 acagtagtac cgtaacgatt ttctttgatc atttgctcag ctagtttttt gtcttcattt
     5701 tcccgagcat attctaaaaa gatttttttg ggcacaccca tccataatct tgctttatct
     5761 gattgattcg ttccataggt cgtttcaaca aagggtttgt tgaacactcg atcttctgtg
     5821 acaaaacgag ccagattcaa gccgttcaat gtcacaaaga aactgctacg accttgtctc
     5881 aaattgattt caaataattt atattctcca tctctaggat catacttcat atcgaaattg
     5941 gcaaagcctg tatattcgat tttttccaaa aatgccttaa tggtttgata gattttttcg
     6001 ttataatctg gcatgatcac aacgtaatta ccaatcgatg ctggtgtagg atcttctaat
     6061 agtggatgac caaggcacat catccgaact tgatgatctt catctacata cgcatttagc
     6121 acacgcatat tactatcatc acccgggata aaatcttgta caatcatttc acttttataa
     6181 ccggcttcgt atatccggcc taaaattaaa tcgaactctt ctctagtctc taaaataaaa
     6241 gctttttttc ttccttcaaa ttggacagaa agatactcca cactgtttgc aggtttcaaa
     6301 gcaactggga attcaaaagg caattcctgt tccaaatgtc catttactaa catttcttct
     6361 ctgacgatca atgttttagg atacggaaga tcatattctt cacaaacttc ataaaagctt
     6421 actttattga tcagcctttc aaataaagaa taatcaatat atggacagat aaaatattct
     6481 gacagctctt gtttatgttg ggagatcaat tctgcatatc catcaccaca agcaatcaaa
     6541 agatattttt tactcttatc agaatatctt tcttttgcca aacgaagcat cgtctcgata
     6601 aatacagggt ctttatcaaa tcctggaata acttctacat tgacaatttt actgtagcgg
     6661 gtaggggcta actgatccga agcatatgct tggcagattt tgccgtatgc ttcattaaaa
     6721 gaacgtgcca taccataaac gttcatgtcg cttcctaata atatagggat aaattcttca
     6781 ttttcaatac tgttcatcat tatctctcac ttactcataa tctcttgttt ttcttttcca
     6841 actacacaag gaatatcata ccataaagaa aaaagcaatc aaaggaaact cctccgattg
     6901 ctcaattctt ttttgaacgg attgaactta taggaagttt ttatctatac caatcagtgt
     6961 agaaagaact accatgtaat gattctagaa cactgatatt aataaaacaa gcaaaaattc
     7021 atttttttat gtttttttaa ctatctcagc tatctatcct ctttatacca ttattttttt
     7081 caaatagtga cctgtataac tttcttttac gttaatgatt tcttctggtg ttccagtcgc
     7141 aaggatcgtt cctccacctt ctccaccttc tggtcctaaa tcaatgatat aatctgctgt
     7201 tttgattaca tctagattgt gttcaatcac taaaactgtg ttaccagcat ctactaatct
     7261 ttgtaataca tgcaacaagc gggcgatgtc atcgctatga agtcctgtcg ttggttcatc
     7321 tagtatatag aaattctttc cattagagat tttgtgcaat tcactggcaa gtttcatccg
     7381 ttgtgcctca ccaccggaca atgtcgttgc tggttgcccc atcgtcacat aacctaagcc
     7441 aacatcaaca atcgtttgca gtttgcgatg aatctttgga atgtgcttga agaattctac
     7501 agcatcctct actgtcattt ccaaaatatc agcaatgctt tttcctttgt aatgcacttc
     7561 taatgtttca gaattatatc gtttaccatg acatacttca caaggaacat agacatcagg
     7621 caaaaagtgc atttcgatct taataattcc atccccgcga caagcttcac aacgaccgcc
     7681 ttttacgttg aaactaaaac gccctttctt ataaccccgc atttttgcct cgttcgtttg
     7741 agcaaataac ccacggatat catcaaatac acttgtataa gtcgctggat tacttctcgg
     7801 cgtccggccg attgggcttt gatcgatatc gatgatcttt tcgatacttt cgtagccgga
     7861 aattgtcttg aatttacctg gcttagcaga attcttattt aatttttgcg ctaacgattt
     7921 ctttaagata ctattgacca atgtactctt tccagaacca gacacacctg tgactgctac
     7981 aaattctcct aatggaaatt caacactaat atttttcaga ttgttttctg aagcaccagt
     8041 gatcttgatt gctttcccat tacctttacg gcgttctttt ggtacaggga ttgatttttt
     8101 ccctgaaaga tatttccctg tcaaagaatt ttcatcatta gctacttctt caggagtgcc
     8161 tgcagccacg atttgtccgc cttgttctcc ggccccaggt ccgacgtcaa tcaaataatc
     8221 tgctgcccac atcgtatctt catcatgttc gactacaacg agggtgttgc caagatcacg
     8281 cattttcttt aatgagccaa tcagtcgatc gttgtcgcgt tgatgcaatc cgatcgatgg
     8341 ttcatctaaa atatacaaaa ctccagaaag attcgatcca atttgtgtag ccaatctgat
     8401 tcgctgcgct tctcccccag ataatgttcc ggaagcgcga ctcaaagtta gatagtctag
     8461 tcccacattt tccaagaatg aaagtctgtc atttacttct tttaaaatcg gtttggcaat
     8521 tactttctct tgttcagata acgttaagtt tgagaagaaa tctactgctt tttgaataga
     8581 caactcattt gtttgcccga tattcgttcc attgatttga acagatagag cttggggatt
     8641 caatcgataa cctttacatg tctgacaagt taattctgtc atataaagac gcatttgctc
     8701 acgcgtgaaa tcactgttcg tatcatgata acgacgctcg atattcttca ataccccttc
     8761 gaaagtcgtt tctacatcgc gaacgccacc aaaatcattt tcataatgga aatggaaaag
     8821 atcatctgtc ccattcaaga tgatctcctg atgctcttcc ggtaattctt cgaatggtgt
     8881 atctaaatcg ataccgaaac tttcacaagc ctgcgctaac atttgaggat aatactgaga
     8941 gctgattgga ttccatggaa cgatagcacc ttgactaagt gtcttcgttc tatctggaat
     9001 cactaaatct tgatcgactt ctaatttgac acctaaacca tcacagtctg gacaagctcc
     9061 aaaaggcgca ttgaatgaga acagtcttgg ttctagttcg ccaacagtaa atccgcaata
     9121 aggacaggca tagtgttcgc taaacaacat ttcttcttca ccgatcacgt caaccagagc
     9181 atagccgtct gctaatcgca gggccgcctc taaagaatca aataggcgag aacgaatccc
     9241 ttctttcaca atgatccggt cgatgacgat cgcgatatca tgttttttgt ttttctccag
     9301 ctcaggcact tcacttaaat catagatctc accatcgact cgaacgcgga cgtatccctc
     9361 tttttggatt cgttcgaata tttttttatg ttgtcctttt ttctttacca cgatcggtgc
     9421 caacacttgg atctttgtgc gctcaggcaa agccattact tgatctgcca tttgatctac
     9481 tgattgactg gtaatctcga tatgatcgtt gggacaaatc ggatgtccta cacgtgcaaa
     9541 caacaagcgt aaatagtcat tgatttccgt cacagtacct actgtcgacc gaggattttt
     9601 acttgttgtt ttctgatcaa tagaaatcgc cggactcaat ccgtcgatgc tatctacgtc
     9661 cggcttatcc atttgtccta aaaactgacg agcataagca gaaaggctct ctacgtatct
     9721 ccgctgccct tcagcgtaca gcgtatcaaa tgctagggaa ctttttccag aaccggacaa
     9781 acctgtaacg accacgaatt tatctcgtgg aatcgttaca tctatatttt tcaagttatg
     9841 ggcacgtgca ccatgaatcg taattttatc gttagccatg ttgtcctcct actttgaagc
     9901 ttttaattct aaaatcatat ctcgcacatt cgcggctgtt tcaaaatcca aagcttttgc
     9961 cgcatctttc atttcttttt ctaatttcat caataacgtg tctttttctt ccttcgtcat
    10021 ttcttcgtac gagacttgta ccgcttcttt tgtatcatct tccgattctt tcgtaataga
    10081 aatcaaatct cggatttctt taatgatcgt ttttggaacg atcccatgtt cttcattgta
    10141 cttctgctga atcgtacgac gtcttgacgt ttcatccatc gctagacgca tagaatctgt
    10201 tactttgtct gcgtacatga ttactttacc ttcttcattc cgagcagctc gtccaatcgt
    10261 ttggactaag gaacgttcac tacgtaaaaa gccttcttta tccgcatcca agatagcaac
    10321 aagggaaact tcaggtacgt ctaatccttc acgaagcaaa ttgattccaa ctaacacatc
    10381 aaattcacct aatcgaagat ctcgaatgat ttctgttcgt tccagcgttt tgatatcact
    10441 atgcaaatat ttgactttga tccctaattc tttgaaataa tctgtgaggt cttctgccat
    10501 ttttttcgtc aatgtcgtta caaacacccg ctggtctttt tcgactcgtt catttatttc
    10561 gcctactaga tcatcgattt gtcccatgat tggacgaact tcgatcactg ggtcaagcag
    10621 ccctgtcgga cggatgattt gttggataac ggtatctgtt tgttcatgtt catatggtcc
    10681 tggcgtcgca gagacataaa tgatctgatt gacatgtttt tcaaactctt ctaaccgaag
    10741 tggccgattg tctaaagcag atggcaaacg gaaaccgtaa tctactagca tctgttttct
    10801 cgctcggtca ccattataca ttcctctgat ttgaggcatg gttacgtgcg attcgtccgc
    10861 cacgatcaaa aaatcttcag gaaagaaatc caatagcgta taaggcggtt caccttcttt
    10921 acgtccgtcc atatgccgag aataattttc aattcccgac gtgtagccca tttctaacat
    10981 catttcaata tcgtaattgg ttcgctgttc taaacgttga gcttccagaa gtttgttttc
    11041 atttcgcaag acagttaaac gctgttctaa ttccgctttg atattcgcta cggcatgctc
    11101 catatgttca tcattcgtca caaagtgagt cgccgggaag attgccacgt gttccgtttc
    11161 tcctaaaact tcacctgtca gtgcattgac ttctcggata cgttcgacct catctccaaa
    11221 aaactccaca cgtaaggcac gttcatctct tgaagccgga aaaatctcca cgacatcacc
    11281 gcgcactcga aaacgaccgc gctgaaaatc tatatcattt ctttcgaatt ggatactgac
    11341 taagtcacga atcaactgat tgcgatctag ttctgctccc tgtcgaatcg aaactacttg
    11401 tttttggtac tcaaaaggcg aacctaatcc aaaaatacat gaaacggatg caatgacgat
    11461 cacatcattt ctttctaata atgaactagt agccgaatgt cgtaatttgt cgatctcatc
    11521 atttacactt gaatcttttt cgatatacgt atcgcttgaa ggcacataag cttcaggctg
    11581 ataataatca taataagaaa caaaatattc aacagcattg ttagggaaaa attccttgaa
    11641 ttccccgtac agctgaccag ccaatgtttt attatgagca ataatcagtg ttggtttatt
    11701 cactttctca ataagattgg aaatcgtata tgttttccct gttccagtcg cacctaataa
    11761 gatttgtgct tttttcccgc cgactacgcc atccactaat tggttaatcg cttcaggctg
    11821 atcaccagcc ggttggtatt tcgagacaag ctcaaagtgt cgggaggtat ctctctcaat
    11881 cattcttcgt cctccttttc attcattcct tttcatttta cgaccaataa aaagaaaagg
    11941 aaagcaaagg cattccttac taaaaaatta taccacaccg aacatacttt cgccactttt
    12001 ttatcttatt atacatgtta cgttttataa cataaaatgt tagaaactcg catttttttc
    12061 attttttcga gcttacagtc ttgtctgatt cagcactttt cgcctataat aataaaatat
    12121 tggaaattta gga
//
LOCUS       DAJSMI010000078        12127 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJSMI010000078 DAJSMI010000000
VERSION     DAJSMI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.078"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            44..946
                     /gene="gnd"
                     /locus_tag="ON430_001679"
     CDS             44..946
                     /gene="gnd"
                     /locus_tag="ON430_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCR3219597.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            948..1949
                     /locus_tag="ON430_001680"
     CDS             948..1949
                     /locus_tag="ON430_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCR3219598.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1946..2230
                     /locus_tag="ON430_001681"
     CDS             1946..2230
                     /locus_tag="ON430_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR3219599.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2263..3060
                     /locus_tag="ON430_001682"
     CDS             2263..3060
                     /locus_tag="ON430_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3219600.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3074..3907
                     /locus_tag="ON430_001683"
     CDS             3074..3907
                     /locus_tag="ON430_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3219601.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3925..4782
                     /locus_tag="ON430_001684"
     CDS             3925..4782
                     /locus_tag="ON430_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3219602.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4804..5313
                     /locus_tag="ON430_001685"
     CDS             4804..5313
                     /locus_tag="ON430_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3219603.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5350..5751
                     /locus_tag="ON430_001686"
     CDS             5350..5751
                     /locus_tag="ON430_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219604.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5863..6216
                     /locus_tag="ON430_001687"
     CDS             5863..6216
                     /locus_tag="ON430_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219605.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6250..6693
                     /locus_tag="ON430_001688"
     CDS             6250..6693
                     /locus_tag="ON430_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR3219606.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6710..7795
                     /gene="uxuA"
                     /locus_tag="ON430_001689"
     CDS             6710..7795
                     /gene="uxuA"
                     /locus_tag="ON430_001689"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3219607.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7788..8501
                     /locus_tag="ON430_001690"
     CDS             7788..8501
                     /locus_tag="ON430_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3219608.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8708..8857)
                     /locus_tag="ON430_001691"
     CDS             complement(8708..8857)
                     /locus_tag="ON430_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219609.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9253..9453
                     /locus_tag="ON430_001692"
     CDS             9253..9453
                     /locus_tag="ON430_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3219610.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            10021..10584
                     /locus_tag="ON430_001693"
     CDS             10021..10584
                     /locus_tag="ON430_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219611.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(10645..11010)
                     /locus_tag="ON430_001694"
     CDS             complement(10645..11010)
                     /locus_tag="ON430_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219612.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(11086..11331)
                     /locus_tag="ON430_001695"
     CDS             complement(11086..11331)
                     /locus_tag="ON430_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3219613.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(11401..11655)
                     /locus_tag="ON430_001696"
     CDS             complement(11401..11655)
                     /locus_tag="ON430_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219614.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(11819..12124)
                     /locus_tag="ON430_001697"
     CDS             complement(11819..12124)
                     /locus_tag="ON430_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219615.1"
                     /translation="MPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLV
                     MMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT         4155 a         1909 c         2584 g         3479 t
ORIGIN      
        1 aatttttttg tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat
       61 tggtttggga aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt
      121 acaaggactc gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc
      181 ttatcaagag atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat
      241 gcttcctgct ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc
      301 aggagacatt attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga
      361 agagtttcag aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg
      421 agcacgacat caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga
      481 acaattattc aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag
      541 cggacacttc ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg
      601 tgaaggattc caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg
      661 tgtttggaat catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt
      721 aaacgaacat cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa
      781 atggacggtt gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt
      841 gttcatgagg aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact
      901 aagaaatggg tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc
      961 atatagcaat cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat
     1021 taaagtcagt tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac
     1081 tagcggagtt acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac
     1141 gagatttttt tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca
     1201 acaatattga tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt
     1261 tagttgaacg aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac
     1321 gcacgacaca agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg
     1381 tcggtaggac gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg
     1441 gagtaatcga aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca
     1501 aatcagattt atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg
     1561 aacaatcaga tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt
     1621 ctgataaaga agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg
     1681 ctctagttga tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg
     1741 ccacagatgt tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac
     1801 aagtagtgat tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg
     1861 aaaaatgtgt cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc
     1921 agacaaagag caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa
     1981 aacaggccct caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt
     2041 gaaatcatac ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc
     2101 gctacctaaa ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc
     2161 tggagaatgt agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc
     2221 gatttcgtag ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat
     2281 cttgcaagaa tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca
     2341 cctcttgctg tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac
     2401 agacaagctg ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat
     2461 ttcattgccg ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa
     2521 caagattttt tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc
     2581 aaagaagaga ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg
     2641 acatcggagt tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga
     2701 ttaggagata aatacgcaag aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa
     2761 tatattccgg aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat
     2821 caactggcac tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa
     2881 ttagaactgc cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata
     2941 gataaaatgg ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta
     3001 acacagaaat cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga
     3061 aggaggagga cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt
     3121 agccgcggtt atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc
     3181 tttgatgact ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt
     3241 tacagccgct ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc
     3301 tgaaccatct attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc
     3361 agaagcagcg attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt
     3421 tcgttttttc atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa
     3481 tacaaaagct atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt
     3541 cgttccgctt gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa
     3601 agctttagag ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc
     3661 aatcggtatc gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt
     3721 ccttgcttat tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc
     3781 catttttggg attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca
     3841 gcctgccaca agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg
     3901 attttaattt aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct
     3961 gaagaaatca cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa
     4021 attcctcact cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc
     4081 ttgaaaaaac tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg
     4141 ttcttcaaca ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg
     4201 gaacagcaaa gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg
     4261 atgtataaca cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat
     4321 tcaggaaccg tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt
     4381 cctttaggcg cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta
     4441 tttttcgcaa gacaagcttt cactatgggc cgtaatgcta caggcctaat gcaaaacact
     4501 ggtgttcaga cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt
     4561 gcgggaaact atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt
     4621 gttatccagg aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg
     4681 ggtgtgtact tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg
     4741 actggaatat taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat
     4801 aagatgggtg tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg
     4861 gtaacgttat ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca
     4921 aaagataaat ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca
     4981 attgtcatta caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac
     5041 aattgtattt tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc
     5101 cccatggaag aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta
     5161 gtgacgaaat ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa
     5221 caatatggcg taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta
     5281 aaagatgtat tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag
     5341 ggcgaaaaaa tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt
     5401 taccgtaagc gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat
     5461 atttacagta tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgatca
     5521 aaaaatattt atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat
     5581 acacccgttc atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa
     5641 acaatctact ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg
     5701 atgatatata acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc
     5761 actgtactag catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct
     5821 agcagacaaa cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt
     5881 acgattgatg atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag
     5941 ggcttagaca gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct
     6001 gttggggcag atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtgg
     6061 ctgaaacaag ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa
     6121 aagcaagcgc agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg
     6181 caaaatgcca atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg
     6241 agaagactaa tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa
     6301 gatgcggcgg aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct
     6361 ggggaggaca tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca
     6421 gcagaaccca tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc
     6481 ttggcagtca atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg
     6541 aatctgccaa tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa
     6601 gcggtggaac aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg
     6661 gaagaaaaaa aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg
     6721 atttagatgg tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc
     6781 gggaataaat gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat
     6841 ttcagaaatt gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat
     6901 tgaaagtgta gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat
     6961 tgataactat atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta
     7021 tagcttcaaa ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg
     7081 aagctattct cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta
     7141 tcaattgata cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt
     7201 gaaaaaattc gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga
     7261 aaatctgact tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat
     7321 ggctatccat ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa
     7381 tcttggcgac ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct
     7441 gtgcacagga tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt
     7501 aggttcgcgc atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt
     7561 caaagaaacg gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc
     7621 gttggtgcaa gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg
     7681 tgaagtggct atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca
     7741 aggtctatat gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca
     7801 agctcttcat ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg
     7861 cttatgtaaa atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac
     7921 taactggcca aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg
     7981 ttccatatat caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag
     8041 ataagagagt atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag
     8101 gaatccatac aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa
     8161 tggaaaatct atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa
     8221 agccggaacc agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag
     8281 cacttgtatt tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt
     8341 cttttctcat caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga
     8401 aagtagatgt aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc
     8461 aagcaaaacg atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta
     8521 gtcctcttta tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt
     8581 attttgaagt atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta
     8641 ctgttatcta ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt
     8701 ccactcctta tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg
     8761 ctacatgatt ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg
     8821 ttactcgata ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata
     8881 cttgctgaaa ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat
     8941 tgaaaggttt tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa
     9001 caagaaaaca aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa
     9061 tagaagacaa tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga
     9121 tatattaaat gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa
     9181 actccttttg cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg
     9241 aggaatttat ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc
     9301 tttatcactg gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac
     9361 ggcttcaaaa ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt
     9421 ggccctcaag caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc
     9481 gaaagaaaag acggtttttt tgtacgaaaa aattcattgg tcgttgaatg ggctgaatag
     9541 tagtgtataa aacaattttg tggagcgtga agcttttatg gtagaggaaa atacgaataa
     9601 agtgatgttg ataaaaagat tcctatcttg aatatgaatc gagatgtctt gaaaatgaat
     9661 gagtttgacc aatcattatc ctatcgaaat ttacagtttc gttcgattgg tctgtcaatt
     9721 aatgaaaaag taaaaaagaa tatgttctag gaagagggcg gcacatctaa atttttaagt
     9781 aacggacagt tcaaatatca agcattgaaa gaggctatcc tactgtttga aggaatacga
     9841 atatataaaa acgacaaaga ggaaaaagta gttcattaaa tcccttcaag tgatgaaaga
     9901 atgagatcta ataaaacctg atctttcatc tgttttttta ataacgtaat aattttttta
     9961 ttttttgtgc tttcgccgtt attgtagagt gatttagcat tcttattttt aaaaataact
    10021 gtgttatata ttaaaaaagg cataatagtt ataagctttt ttaaagaata tttagaaatt
    10081 attaattttc gtctgaaaac aaaaaaagaa agcgttatta ttttagtcta ttttcagggg
    10141 gggaacctta tgataaaaaa actattttta ctatcaatat taacgctagc aatggcaggt
    10201 tgttctgact ctggcttagc aactgaactg atcacaaaat caactgaagc cacgacagag
    10261 gagttaaaat gtggaaacga tcacagtgac tacagaggct taaatcgacg atgcatggga
    10321 atattaggca gaagcaattg taagcatcga tgtagtaatc gaagaaggtg tagaagaagc
    10381 aacagaaaaa tcaaaagaaa gatcaaaaga agcaatctgt gctgtgcatc gtatacagac
    10441 gtcgaagatg cggtactttt aaaccacgtc aggagatgtt catttatgtc tagggaagtt
    10501 ggcgatgaac caaaactaga gtacgctttt agttttgcac gtgaggatga agataaccct
    10561 aaaacttacg acattttagc ttgatctaag atggttaaag ctttttaatg aagtaagagg
    10621 agatagaagg cttttctagt tatattaggt aagataaatt cctttttgct ccaaatttgc
    10681 taaacattct gctagataaa cttcatcgtg ttccggataa atcggcacgt tgcgctctac
    10741 agtaggtaaa tcaaaaaatg cttcgcatga tttgccacga tacataggat ttttccactc
    10801 tggtgctggg ctgtaaccac gtttttccat ttcatccatg acaagctgat gatattggaa
    10861 taatctataa ggagaatagt gaaaaacata atccacagta gcatgtttct ttccccagcc
    10921 atttcctctt aatgcacagc attcccgatg ttgtcctaaa agctgttgac gaggaagctt
    10981 tgaaatcaat ttttcatgcc ataatcgcat agaaatgtct cctttatata tagattgtta
    11041 accttagacg ttaaatgaaa aaaccagcac caaggctgga aaaaatcact tcagtttgct
    11101 tgctgtttct gagcgaccaa ttaggtaatc aacggaaata tcgtaaaagt cagcgatata
    11161 gattaattca atgatgcttg gaatttcttc tccttcttcg tacttacgat atttgaataa
    11221 aggaatactt aacgcgctag aaacagctac ttgagataaa gatcggccag ttcttaagaa
    11281 attcagacga caacgtaaag tatccagtaa aatagcttct tcgatactca tttaatcacc
    11341 tttcattata gtagaatagt tataatgact atagaaagct acacatgcaa tcaatagtac
    11401 tcaatgtttt ttatttaaac gtatcaaata aagaatgacg atcaataaag acaaaaccag
    11461 tcctgataat tcttctataa aaacactatc gggttcaaag tagttgtaat taaaaacttt
    11521 ccacataaag tagatagccg gaagagtcaa aatagctaaa taaaaccgtt ctttgttcca
    11581 attcataaga taaacaatac ttgcgaccag tgcgaccgca ggcacccaaa acaaaactct
    11641 caccaacgta atcatgtaac ggactcctca acgattcata taataaaaga taacatgtta
    11701 ttgcatacat cacaaatgaa atgctaggaa aataaaagaa gaatagatca atgaaaaatg
    11761 aaaaagcaga acagttttct gctgataagc aggaactgtt ccgctgaatg tgtcttatct
    11821 atcgagaaaa aatcaatgga ttgatatcta agtaattcaa tacatataaa aagaggacga
    11881 tcgcattcac taaaaaggaa agaatgatga tcccatagcg aatggaaaca tatttaacaa
    11941 atacttttgt ttgaatacgg gcatatcttt ttcctttata gctgagcatc atgacaagaa
    12001 gcaaaagggt aatcacacct tgaaaaatca ttccaataaa cagaattcct gaggtttgat
    12061 aatttaattg gagaaaataa gctgctgccg caactaaatc gaataaaatc gcaataaatg
    12121 gcaaaat
//
LOCUS       DAJSMI010000079        12076 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJSMI010000079 DAJSMI010000000
VERSION     DAJSMI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.079"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            6..410
                     /gene="tnpA"
                     /locus_tag="ON430_001698"
     CDS             6..410
                     /gene="tnpA"
                     /locus_tag="ON430_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HCR3219616.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            427..1575
                     /locus_tag="ON430_001699"
     CDS             427..1575
                     /locus_tag="ON430_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3219617.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNKLSTEIIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKVDKWFPSSQICS
                     NCGHKDGKKSLEIREWTCPICHTHHDRDINASINILTEGLRLHSMGLA"
     gene            1766..1903
                     /locus_tag="ON430_001700"
     CDS             1766..1903
                     /locus_tag="ON430_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219618.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESY
                     C"
     gene            2345..2725
                     /locus_tag="ON430_001701"
     CDS             2345..2725
                     /locus_tag="ON430_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR3219619.1"
                     /translation="MSEQNQNLASNYSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            2707..3621
                     /locus_tag="ON430_001702"
     CDS             2707..3621
                     /locus_tag="ON430_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR3219620.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            3581..3859
                     /locus_tag="ON430_001703"
     CDS             3581..3859
                     /locus_tag="ON430_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219621.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTIDS
                     QVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLIMTLFAFWLVWGE"
     gene            3866..4555
                     /locus_tag="ON430_001704"
     CDS             3866..4555
                     /locus_tag="ON430_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219622.1"
                     /translation="MAFYLPYLLIFASILGIIGGGYKAYQTRYSLKGSRIRFKRFLLL
                     GVVFTLSIVSSGLFGVLEGNKRVSRSVLLENVTQKYEVAHEKKKKEQVLAQKIEKFTA
                     CYEDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4675..4761)
                     /locus_tag="ON430_001705"
     CDS             complement(4675..4761)
                     /locus_tag="ON430_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCR3219623.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            5407..6339
                     /locus_tag="ON430_001706"
     CDS             5407..6339
                     /locus_tag="ON430_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3219624.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            6356..6826
                     /locus_tag="ON430_001707"
     CDS             6356..6826
                     /locus_tag="ON430_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR3219625.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQAVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            6910..7214
                     /locus_tag="ON430_001708"
                     /pseudo
     CDS             6910..7214
                     /locus_tag="ON430_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7504..8685
                     /locus_tag="ON430_001709"
     CDS             7504..8685
                     /locus_tag="ON430_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3219626.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNAKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
     gene            8775..9044
                     /locus_tag="ON430_001710"
     CDS             8775..9044
                     /locus_tag="ON430_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HCR3219627.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYK
                     KYPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWTASSRTAKYYYYI"
     gene            9849..10022
                     /locus_tag="ON430_001711"
     CDS             9849..10022
                     /locus_tag="ON430_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219628.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELRLNSNYYN
                     SPNEELFVKETDK"
     gene            complement(10336..10797)
                     /locus_tag="ON430_001712"
     CDS             complement(10336..10797)
                     /locus_tag="ON430_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3219629.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
     gene            10930..11304
                     /locus_tag="ON430_001713"
     CDS             10930..11304
                     /locus_tag="ON430_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCR3219630.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            11358..>12076
                     /locus_tag="ON430_001714"
     CDS             11358..>12076
                     /locus_tag="ON430_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219631.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIIT"
BASE COUNT         4498 a         1886 c         1977 g         3715 t
ORIGIN      
        1 ataacatgga attagataca aataatcatt cagtatttct tctttattac caccttgttt
       61 tagtaacgaa atatcgtaga caagtgattg atgatgaaat atctgattat gccaaaacta
      121 cttttgaaag aatttcagaa tcgtatcaca ttactttagt ggagtggaat catgataaag
      181 accacgttca tattatgttc aaagctcaac ctaaaacaga attgacaaaa ttcatcaatg
      241 cctataaaag tgccagctca agattgataa aacgtgactt tccaagagtc aaacaatttc
      301 tttggaaaga aatgttctgg tctaaaagtt tttgtcttct gacaactggt ggcgcaccaa
      361 ttgatgtgat taagaagtat atccaaaatc aaggaaacaa tcataaatag aaagtaggtg
      421 aactttatgg aacaactaaa agcatacaaa ttcagaatct atccaacaga ggaacaagaa
      481 atcttctttg ccaaatcttt tggttgtgtc cgtaaggtct acaatctaat gcttgatgac
      541 cgaaaaaagg cgtatgaaga agttaaaaat gattcttcta aaaaaatgac ttttccgaca
      601 ccagctaaat acaagaaaga atttccgttt ttgaaagaaa ttgatagcct tgctttagcc
      661 aatgcccaac ttaatttaga taaagcatac aagaattttt ttcgggataa atccgttgga
      721 tttccacgtt tcaaaagtaa gaaaaatcct gttcagagtt atacaacgaa taatcaaaat
      781 ggtacagttg ctttgattga tagtaaattc atcaaagttc ctaaattgaa gtcattagta
      841 agaattaagc ttcatagaca accaaaaggg attatcaaat ccgctacaat atcacgtcat
      901 tctagcggta aatattatat ttctttgtta tgcaaagaag aagttcgcga actacctaaa
      961 tctaattctg ctgttggtat tgacctaggt attatagact ttgcaattct ttctgatgga
     1021 caaaaaattg ataataataa atttacgtcc aaaatggaaa agaaactcaa acgtgaacaa
     1081 cgtaagttgt caagacgtgc cttattagct aaacaaaaag gtatcaatct gtttgaagct
     1141 agaaattatc aaacacaaaa acgtaaagta gcacgattgc atgaaaaagt aatgaatcaa
     1201 cgtactgatt ttctgaataa gttgagtaca gaaattatca aaaatcacga tatcatctgt
     1261 attgaagact taaacacaaa aggcatgttg cgtaatcata aactagcaaa aagtatttcg
     1321 gatgtttctt ggtctagttt tgtgtctaaa ttacaataca aggctgactg gtacggtcgg
     1381 aaaatcatca aagtagataa gtggtttcca tctagccaaa tctgttcgaa ttgtggacat
     1441 aaagacggca agaaatctct cgaaattcga gaatggactt gccctatttg tcatactcat
     1501 catgaccgag atatcaacgc tagtatcaat atcctgaccg aaggtctaag gctacactct
     1561 atgggattgg cttagatcaa ataaacaaga accgtaggaa ctacggggat agcttggtaa
     1621 ataagaggca cctctgttag taaagtaata cgctatcaag tatgctctct tcccaagaag
     1681 ctcccacttc aagcgtaaga aaccgttagg tttagctaag tggtgagtag ttcacataat
     1741 cacttaataa aaaggaggca cccaagtgat tttattgaaa aaagagtatg aaaaatttta
     1801 tgatagactt atttattctt tgcaattgcc caaaaaatta aaccgtcaat tacaaaagac
     1861 ttatgaaact aaatcaactg aagaatggga gagttattgc tagatttttg gtagaagtaa
     1921 caaaaagtgg acatattatc ataatgactc gccgaaagtc tgataaaaaa ttggctaaaa
     1981 atattgctga atgtttaata aaactatcag ttttagctaa atagttagta gtttacacaa
     2041 aaaagcattt atgttttttg ttgcaaagca acaatttcat cttcggtgaa ataagcgaaa
     2101 ctttacaaaa agtggctagc atttgctagc tttttttgta ttgtggagcg tgggttttga
     2161 gggggattca agggggagct tgcgcccctt tgacaagcgg ttatttgcac caaatacgga
     2221 ggtatttggc tagcaaatat agagcttgcc aaaccctttt agtgagcttt actcattgta
     2281 cctttttagg tacaatggta ttgcgaacga tacgtccgta ggacgtactg aaagggtggg
     2341 acttatgagc gaacaaaatc aaaatttggc tagcaattat tctaaaaaat atacctatcg
     2401 atccgagcca aaacaaatca gttttcgagt gagcgaatcc gaatttgcaa agctgaagca
     2461 atcggctgaa gctttgcaaa tgagtgtgcc ggctttcgtg aaagccaagg cacaaaacgc
     2521 gcgtctggtg acaccgaaag tggcgccaga cattgcccag gcaatcgcgc gcgatttagc
     2581 caaagctgga gggaatatca atcaaattgc taagtggtgc aatacacacc aacatgacgt
     2641 tgcttctggt gacgctcaac gtttatccga aaacctaaaa atcatgcaaa aggagctaca
     2701 aaagatatgg caacaattaa ggtaagccga accacttctt gtagtcgtgc aattaattat
     2761 gcagaaccac gagcaaccat taaaacagga ataaactgtg acgtcgatta tgccaaaagt
     2821 gaaatgaaac aaattcgcat gctgtatgga aaagacgacc gtgtacaggc acacttattg
     2881 attcaatctt ttcgaccagg agaaattacc gctgaaaaag ctaatcaact aggggcagag
     2941 tatgcaaaaa aaatagcccc cgatcaccaa atagctattt acacacatac agacaaagac
     3001 catattcata atcacattgt cattaactcc gttaatctgg agactggaaa aaaatttcaa
     3061 gctcatggtc aagcatttct aaacaaatgt tatgacctaa atgacgagat ttgtcttgca
     3121 cataacttac caatcactga gcgaggaaag aagccagaaa aacgaaccat gtctgaaata
     3181 aaattaaaag aaaaaaatga acctgtttgg aaagacgaaa ttcggattgc gatcgatcaa
     3241 acaatgaaaa acaaaaaaac cagaacctat gaccaatttt gtaattccct caaaacattg
     3301 gggattcatt gcttcaatcg cgggaaaaat ttcacttacg aattgatcca caaaaagaaa
     3361 aaagtacgat ccaacagact aggaaaggac tacgaaaagg agacgatttt acatgagctg
     3421 gacagacgag aacgaaccta taaccaacag cgaaccaacg agctacgaag agctcgagct
     3481 agcattcgcg aaactgcaga agaagtacga agagaacaac gaactcttac gcctgaacaa
     3541 ccggaaatta gaccggctcg aagaaaccat gaacaacacg ttgaaagagg aacagaccac
     3601 caacaagaat tatcgctcta aaacgttaaa agacatagaa aacgaaataa aaagacaacg
     3661 tgcttttctt gcgccttata cccaagaaag gatacaaact acaattgatt cacaagtcaa
     3721 aaaactccac caagcgctat tagaccagcg aaaccaagaa caaaaaaagg ctttaattca
     3781 acgatcgatg gatattcttg cccccttatg ttgcttaatc atgacactgt tcgccttttg
     3841 gttggtatgg ggagaataaa aagacatggc attctattta ccctatctac tcatttttgc
     3901 cagtatttta ggaattattg gaggtggcta taaggcttat caaacaagat attcattaaa
     3961 aggctctaga attcgattta agcgtttttt attactaggg gttgtattta ccttatcaat
     4021 cgtttcgagc ggattattcg gcgttttaga gggcaataag cgcgtttcta gaagtgtttt
     4081 attagaaaat gtcactcaaa agtatgaagt agcacatgag aaaaagaaaa aagagcaagt
     4141 attagcacaa aaaatagaaa aatttaccgc ttgttatgag gacatgaatg atatttttgt
     4201 gaagcaagaa aagcgtttga cggataaaaa tatggaaaca ctcactcgac tttatcggaa
     4261 tttgccagaa gaacagcaaa aagagtacca agaaaaatat gaacaagtaa aaaaagacgt
     4321 gcaatatgtg aaagatacga aagtggaaga agcttgtagt gatttatttg gagatacaat
     4381 tcctttttca actagcgaac aagaacgaaa agaacgacaa caatcagtca catatgaacg
     4441 ctatgaaatc ttgcttcaac aagcaattaa catacaaaat ctgactaaaa aagaaacagc
     4501 tttaaattat ttaaaaagcg tcaaggaatg gttagatcag caacagcaaa attgaatttg
     4561 attgaaattc atgttaaact aaacatgcaa atgttcaaac attagataca cgaaagcccc
     4621 agagaggtcg gattctctgg ggcttttttt aggttgatgt cgcctttact gattttactt
     4681 cttgtttcgg ttattcaacc aataactgaa tacagagata caaactccta caagaagggg
     4741 gcaaataaac agttcaaaca tcagatacac ctccttccag tcgcaagact gtcggatagg
     4801 cgacaagtta ttatacccta caaaattaaa tctgtgtggc aattttaagt atctcttatt
     4861 tccgggttgt tagggcttgc cctgaccgcc tgtagggcgc tttaaattcc agataaaggt
     4921 ttttatacta agactgtctt aaaacggctt agacaagctc ttagagcctc aaccctcctt
     4981 tttgaacaat ggttttagca aaggagctgg aggcgactgc gcaggtataa tgtgagagcg
     5041 tagcgatcac acctttttgg cgatcgtagg gagcgaggtt ttgaattagc agtttagaaa
     5101 agttcgcgaa cctgtttaaa gaaaaaataa ttgttttttt aggtgggaaa ttttgtctcc
     5161 ctcttttata atcttttttt aaacctcttt ttaaacctct ttaccgtcgg ctgtgtcctt
     5221 actcccccaa gggatacaaa attttatagg catgaaatta tctgtttata ggcatgaaat
     5281 tatctgttta taggcatgaa attatctgtt tataggcatg aaattatctg ttttaggtat
     5341 tgtttttaga cataatatgt cctaaaatga tgtcaataaa atcatttaga ctggagtaac
     5401 aactaaatgg caaatgaaat cgtaaagtat gaaccagaat taaataccat tcctttaaga
     5461 aaatttaccc caatagaaat gaacttgttt ttttctattg tctctagaat gcgcgataaa
     5521 ggtaatcaaa aaatacgctt ttatttcagc caacttaaag atttaagtgc gtataagcag
     5581 actgcaggag aagtctttat aaaagatttg gcaagaacat acgatcattt gatggattta
     5641 cgtttcggaa gaaaaagcaa tggtggatta acaatagaac gatttacaat gtttactgat
     5701 tttgaaattc atggagaagt cgataaccct tatgtagata taaaagtcca cgaaaaagct
     5761 ttgcctttac ttaataatct agaaagctgg gtacgtttct ctttaacaga atttcgagat
     5821 ttaaatagca gttatgctaa aacaatgttt agattgctaa aacaataccg aactcaagga
     5881 tgggctgaat tttctaagga agatttttta gagctattag atattcctaa aagctataga
     5941 caaacaaata taaatcagtt tgtactcaaa ccaataaaag aagaactaac tccactattt
     6001 aaaggattaa ctattcgtaa aaaatatggt aaaggtcgag gtaaacctgt tattggttat
     6061 cgttttactt ggaaagctga aatcaaccat gctgacgatt tttcaaaagg gaagcaagaa
     6121 gatttacgta taaaattatt taatattgaa cacaatggag aattaacaca aaaagaaaaa
     6181 tggcgtgcta aagatcgaat actaaatctt ccgttaggta ctcatgaagc tgattttaaa
     6241 aaacaacaac aaaccgaaaa agaagaagcg gaaaaacaat caatctcaaa cgagcttaaa
     6301 caagatttat tagaaaactt acaaaactta ttcgattgaa gcataaggag tatttatgat
     6361 agataaaacg atcaaagaac tcgcggaaga atttcatgtt tcaaaacaag caattaggaa
     6421 aaggctatca gctgatttta gagaaaacta tgtgcaagct gttactagga acggagtgaa
     6481 aaccttagtt gtcagctaca aaggatattt gcttttaaaa caacattttt taggtagtaa
     6541 caccactggt aaccaagtag aaaccgttgc tagtaacgat gaaaactcga ttattgtttt
     6601 tttacatgaa cagctccaag aaaaagatac tcaaataaaa caaatgcaaa agttactcga
     6661 ccagcaacaa caactcacct tgcaggcaaa tcaacagatc gaaaaactgc aagaacagtt
     6721 acaattagct tatacggaag aacctgaaga agaggaatta acttctgtca ataaagaaaa
     6781 caacaatgca ataacaacag aaaaaaagtg gtggaaattt tggtaacaat taccaacttt
     6841 ccataaagaa gaaatcaatt tctatagact ctatctttct aagaataaaa aatatgttta
     6901 ggtgatcaaa tgactataag taaagtaatc aaacaaaaaa gaatagaaaa acaactaact
     6961 caagaagatt tagctgaaat gcttttagta tctaaaaaaa ccatttctaa ttgggaaaat
     7021 ggtcgaacaa taccagatac agaaaattta attcagtttg ctaagttatt tgatttatca
     7081 ctagacaata ttttgttagg agaagaaaaa attatggtaa aaaatcatac aaaaacacta
     7141 atactattat ttcattttgt aattttttta agtttagttt tcaccttaat tcagtccgga
     7201 ttcttttatt ttagccattc aattgccact tggttattaa tattttttct aagtttacta
     7261 ttaataatta atacattaat aattaatact ttaattatta tctttaataa acatagaaac
     7321 ttatttcttc acagcatatt tccaattgtt ttattaattt ttctatactt tatataaaaa
     7381 gatagaacta tttagtaaga taaaaaagta gctaattgta attttcgtct aaaactcaaa
     7441 actagtaaaa ataactttat tgtgttattt ttatttataa attatgaaaa aggacctttc
     7501 gaaatgaaaa ataatagaat ctacaaaaat cagttgaatt atgggctttt attgccagtt
     7561 tttctgctat gtatcattgg attactttct ttatatgttg ctttatatca cgactcttac
     7621 actaattcaa aaatcacaaa agaattaatt aaacaagtat tgtggtatag tattgggttc
     7681 acttcagcaa taactattta tttttttaat cctaaaattt tgtggaaatt aactcctata
     7741 ttttacggat tatgcatcat aatgttatta cttctcttga aattctataa tccacaatta
     7801 gcagtcttaa ctggagcaaa aaattggttt tatattgctg gtttttcgtt tcaacctact
     7861 gagttagtca aaataggata cacccttatg ctagctaaaa taaccgtcaa ttatcagcaa
     7921 aaatctatta ctagtaatgc gaaagatgat tggatttatt tacttaaaac ttttattgct
     7981 actcttccaa taggaatatt attatttttt cagaacgatc taggaacaat gttagtcttc
     8041 attgttattt atatttttat tttgtttacg tctaacataa atataaaaat aattgcccca
     8101 ataatcgcca ttggtgttgt tttatcctta atatttttat atttggttat atcagataca
     8161 ggaagagatt tcttattaaa aataggattt cacgactatc agtttaaaag aatagactca
     8221 tggttgaatc catttttaga tccaaatgga tctagctatc agttagcaaa tagtttaatt
     8281 gctattggct ctggcggact tttaggtact ggttttaatg tatcaaacat tcacgttcct
     8341 gttagagaat ccgatatgat attttctgta attggagaaa attttggctt tataggaagt
     8401 tgtcttgtaa tattcctata cttttattta atttatcaaa tgtataaaac atgttttaaa
     8461 agcaataact tatttttcac ctatatcggt ataggcattg ttgcaatgat tttttttcat
     8521 gtttttgaaa atataggtgc aagtatcgga ttactacctc taactggtat tccactcccc
     8581 tatattagtc aaggtggttc tgccattctt agtaattttt tggggttggg atttatgttg
     8641 tgttgtagta gatatactaa cgaaaaaaaa ggaacacaaa aatgaattca aaataacact
     8701 ttatgaatag aatgtctatt tcaataaaaa ttatgatacc ttaaagttaa tcattaaaaa
     8761 aggagtgtca taatttgaaa aaaacaaaat tattggtagc tagtttgtgt ttgtttagca
     8821 gtttattagc tttcacccct tctgtttcat tttctcaaaa tggtggagtg gttgaagctg
     8881 ctgctcaaag aggatatatc tacaaaaaat atccaaaagg tgctaaagtg ccaaataaag
     8941 ttaaaatgtt agtaaatatt agaggtaaac aaactatgcg tacttgttac cttatgagtt
     9001 ggactgctag tagtagaaca gccaaatatt attactacat ttagtaaaaa caaaacattc
     9061 tttctataca ataaaatagc gtatagagag gatgttttta atttgaaaag gagtggttat
     9121 ctttatgaaa aatagatcat ttgctatatt agtattatcc gtaattttat tagatatatt
     9181 gttaataaca ttagctttaa aaactttaaa tttagtaaca agttttgtca ttataccagt
     9241 aattatagga atgtctattt atttcatcaa aaaaaataaa caattttttt gaatgaaatt
     9301 acgggcgaaa ataatcactc aaaattatta ataaaaacga atcaactact ttttctgaag
     9361 aaacatacaa ccgtaaacaa caattgttgt ttacggttgt aaattatcaa actatatggt
     9421 gacttgttgc ttaaaatagg ttgaaatttc gtttattttt tttgagaaat tttacccacg
     9481 tactacccat gtacctatcc acgtattacc catgtacccc ccatgacaaa tgggcggagg
     9541 ggcttgcggc gcttggtatt aaaggcatta tccgcctccc aggtttctga ccattttctc
     9601 tcaacgcggt aaaaacccca cttatgagag aaaatggtca gaaacctaaa cgatcggcaa
     9661 agaaaacata ccaagcgcca caagcaaaaa aagttagagt aacttccaga aaagaaaaat
     9721 ttttagaaca acttttgaaa atttgatttt gatttatatc cgttcaattt aaacatttga
     9781 taaactgaag gctagtattt aaaatgtgta tacaagtggt agcgttaaaa aaagtggagg
     9841 tataacctat gttaaaaaaa actgtgttgg tactgggttc tatttctcta ggaatattag
     9901 gttttagccc catgctatta tccgtaattg gaaaaaacaa ttccaacaga gaacttagat
     9961 taaattcaaa ttattacaac agtcctaacg aagaattgtt tgtaaaagag acggataaat
    10021 aataaaaaag actgctcttc cgagcagcca gttaaaaaaa taatgagagt ttattcttga
    10081 aaaagcaacg cgcttataaa gcgctaccaa aattttatca tattttataa aaaataacaa
    10141 tccagtaaaa aatgactggt aaaaattcac aaagaaacgg aaagaagcaa ctacagaaaa
    10201 tcaagcctta aaaagagctt tttacctaga ccaattaaaa atcacaaagt gagcgtctct
    10261 atccattggt agaggggcaa cttggtctag tatacattat gtcaactatg caattaaata
    10321 actaactact tcatattagc ttaataaaat aatcaaaatc actaggggaa aaaatattgt
    10381 ataaaaaaat ttttcatttt ttaaataaga atagtaaata taaaaataat ttaagatatc
    10441 tattactatt aatccagata gagcaaacca agtaaatatt ccgtgtgtaa agctaaaaaa
    10501 attcatttga atcaattcaa ataaacctaa tatagtaaca aatatattaa aaataatagc
    10561 tgtcaatacc agtaaattat tttgtttatt ttttaaatta tcttcattta aaatagaatt
    10621 tatgttagta ctataaaatt cacaaatttt ttttagtgtc tctatatctg gtgttgtttt
    10681 gttattttcc cactttgaaa tagtttgacg tgtaacatta aattttcttg ccacatattc
    10741 ttgagtatac ccttttttca ctcttaattc tcttagctta gatcctaaat cctccatttt
    10801 ttctcctatg taaacgatta tttacataaa attttatcat aaaaatacta acgcgccaaa
    10861 aaagttcttt attttataaa acacactaaa ataaaataat tctagtaaaa aagataggag
    10921 aaataagtta tgaaaaaatt tttatgtctt tctgctttaa tgggtgtctt attaactagt
    10981 ggaggaatag ttagtgctac agaagcttta aatctagatg tacccgaaga ccataccgcc
    11041 atttacggag gaggaatgga agctacagaa gaaggatttt cttctagtaa attgagatat
    11101 gctgctggcg gcggagattt caactgtggg gttaatggat ttaaagttta tgcaaattac
    11161 taccatgcta gagctaaaca tagtgcaaca gctaaaaatg gacgaggcgg tcaagtacga
    11221 tcagttcaga aagctggagt aagagcttat gcaacatgca atgccacact tactggaaat
    11281 actggttggt ggaatgttta ttaaaaaaga gagtttaaac tctctttttt atttacattt
    11341 gaaaggaaat tcaaaatatg ttaaaaaaaa taaacgctgt tttatcactt tttgtctgca
    11401 ttacaatact attcttttct actaatgtta taaatggtat aaagacttct gaagcgctat
    11461 ataaaaatac cgctgaaatt catatgcaat ataaagatga tatggacttt aacagttata
    11521 taaacgcttt gtatgaaata tctacacaag aaaatattaa tatttctcaa tatagtttta
    11581 ctgctgttaa ccaactaact attcttgcta caaaccctaa cgctaacaaa cactggaaat
    11641 tttctaaaaa aaatatatta gcaagaaacg attctaaagt ccattatact aataacaaaa
    11701 attctaagta ccaattaaaa cttccaaata attttttaaa tataaatatt tatccttttt
    11761 ctcatgtgga aaatattgga ttaagtgaaa tattatatgt acaaggaaac tcaaacaaat
    11821 taattcctgt actgaaaaaa tatggaacac ctactatttc caatatcaat caatcagata
    11881 cttttcaaat taatactaat attgttcttg ttatctgcta tcttagtata tttattgtaa
    11941 ttactacaat tatatttgct ttttcaaaaa tgaaagaaat cactctaaaa aaaatgttag
    12001 gctataactc ttttgatact ataaaagatt ctattaaaga gttaaacctc ttattttgtt
    12061 ctttgattat aacttt
//
LOCUS       DAJSMI010000080        11946 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJSMI010000080 DAJSMI010000000
VERSION     DAJSMI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.080"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(32..1978)
                     /locus_tag="ON430_001715"
     CDS             complement(32..1978)
                     /locus_tag="ON430_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HCR3219632.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFVFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVYSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            2163..3602
                     /locus_tag="ON430_001716"
     CDS             2163..3602
                     /locus_tag="ON430_001716"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR3219633.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3604..4566
                     /locus_tag="ON430_001717"
     CDS             3604..4566
                     /locus_tag="ON430_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3219634.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(4736..6162)
                     /locus_tag="ON430_001718"
     CDS             complement(join(4736..5589,5589..6162))
                     /locus_tag="ON430_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HCR3219635.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSPVQFRKTTVTVV"
     gene            6405..6860
                     /gene="tnpA"
                     /locus_tag="ON430_001719"
     CDS             6405..6860
                     /gene="tnpA"
                     /locus_tag="ON430_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR3219636.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            7125..7223
                     /locus_tag="ON430_001720"
                     /pseudo
     CDS             7125..7223
                     /locus_tag="ON430_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(7446..7676)
                     /locus_tag="ON430_001721"
     CDS             complement(7446..7676)
                     /locus_tag="ON430_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HCR3219637.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(7906..8724)
                     /locus_tag="ON430_001722"
     CDS             complement(7906..8724)
                     /locus_tag="ON430_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3219638.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            8885..9574
                     /locus_tag="ON430_001723"
     CDS             8885..9574
                     /locus_tag="ON430_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR3219639.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            9588..11081
                     /locus_tag="ON430_001724"
     CDS             9588..11081
                     /locus_tag="ON430_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3219640.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILG"
     gene            11103..11579
                     /locus_tag="ON430_001725"
     CDS             11103..11579
                     /locus_tag="ON430_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3219641.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         3968 a         1928 c         2115 g         3935 t
ORIGIN      
        1 gaaaattcca tctttactca gggaaataca tttatttgat ttcaagtaaa tcagtaccct
       61 cagtgactgt attttcagct actatattta cttctgaata atccgaagta ttggtcacaa
      121 taatcattgt tgtatcatca agaccttctt tctcaatcat tgaacggtca aatgctccaa
      181 gaatatcgcc ttttctaact ttttgattag tagtaacttt ttgtgtaaat ccttttcctc
      241 ccattgatac agtatcaata ccaatatgaa ttagaacttc agcaccatta tttgatttaa
      301 taccataagc atgtccagtt tcaaacgtga gttgaatagt accatctact ggagaataga
      361 ccgtgtttcc atttggttta atggcaatcc cttttcccat agcttctgat gaaaagactg
      421 gatcatttac tgaagaaaga ctaatcactt taccagaaat gggactaaca attgtttctg
      481 atgaaacttt tgtttgtgtt gattcaacat cagtagatat tggatctgtt gttgctactt
      541 ttgaagaatt aactggtgtt tgttcagcaa tttcttctgc tttaaagata ccagcagctt
      601 ttgttttacc gtagaagtaa gtaattgcaa aagcaactac aaagctaatc aattcacaca
      661 atacgtaaaa tggaatagat gttgcattaa ttgaaagaaa tcccaagaaa cctgctgaac
      721 caagtgaaac agcaactact ttaagtaatc ctgctactgc tgtagcaatt cctgaaccaa
      781 ttaaagcaca aaagaatgga aagcggtatt ttaagttaac tccgaacaag gcaggctctg
      841 taattccaag aagtgctgag acacccgcag aggaagcaag actttttgtc ttagaatttt
      901 tagttaagaa ccaaatggca aatgttgcag caccttgagc aacgtttgcc atagatgcaa
      961 ctacaaagac aaagtctcca tgtccgatac catttgtcca cgctgaaagt aattgagttt
     1021 caatagctgg aaatgattga tgtaaaccag tcatgactat aagtgaatat gttcctccaa
     1081 aaatacccat tccaataaat ccagtagtgt tatatagcca aacaataatg tctgtaattc
     1141 cattagacaa caataacatt acagggccaa taactgtgaa agtcaaaaat ccagtaatca
     1201 ttactgataa taatggcgtg aatgtaaagt caatagatga tggcaatttt ttatgaaaga
     1261 atttttctaa tatagataat atccaaacag ctgctaaaac tggaataact tgataagcat
     1321 agtttgtttg tgaaacatga taaccaaaaa tattccaata ttcagttgag ccaccaagat
     1381 ctggtgttgt catgatcata ccaattgcag caccaagaaa ttggtttgct ccaaaacgtt
     1441 tagcagctga aatacctact aaaattggta agaacatgaa tggtgcagcg gacatcagtt
     1501 ggatcattga ggaaatacct tcaatcgcag gatacatttc ttcaattgat tttggtccaa
     1561 aaagtccagc agctgttaaa aagttttgta atgccattaa taaaccacct gcaacaaggg
     1621 caggtataat tggaacaaaa atatctgata ataatttaat taatgccata atggggttga
     1681 attttttatc tttactagca atttgtttta aatcatctgt tgaaatttcc gaaagtccag
     1741 tttttttgat aagttcgtca taaacgaaat taacatcacc tgcaccgata atcacttgat
     1801 attgtccatc aattttaaat gtgcctttca catctggatt ttcgtctaaa gctttttgat
     1861 tcactttcgt gtcatctttt agtacgagac gaagacgtgt agcgcagtgt gcagctgcaa
     1921 taaggttatc ttttccgacc gcttgtatta cgtcggaagc tactttactg taattcattc
     1981 gaatcactcc ttttttattt ttacgtgaaa acggttcact gaaatgcaaa tttattttag
     2041 catatcttaa aaatatgtca aacgtttgtc atatagttta tttttttaaa tgaaatgatt
     2101 gatttttcaa cggaaaacgt ttaccatact agttgtaaaa agataaattg aaggagtaaa
     2161 atatgaactt acctcgagaa atacgatatc gtgcttattc tgattggact aaagatgaga
     2221 tggataaaat taaggataat gtaaaacgat caccttggcg tgctagctat catatcgaac
     2281 ctaaaacagg attgttaaac gatcctaatg gtttttcttt ttttaacgga aaatatacgc
     2341 ttttttatca aaattggcca tttggagctg ctcatgggtt gaaagaatgg gtacacaatg
     2401 aatcagatga tttagttcat tttcatgtaa caggagcaga acttcttcca gacacaaaac
     2461 gagatagcca cggcgcttat tccggttctg catatgaaat agaggggaat ctattcttac
     2521 tctatacagg aaacgttcga aatgaaaatt gggaacgtga tacacttcaa atcggagcat
     2581 ggatggataa agactacaac attacgaaag gtgagaatgt tcttattcat aaacctgctg
     2641 atgtaactga tcattttcgt gatccacaaa tgtttaatta tgaaggacaa ttgtatgcga
     2701 ttgttggagc acaaaattta gaaaaatcag gctttatcaa actttacaaa gcggatgaca
     2761 ataacgtgga aacatgggag gaagttggca accttgattt tggcggcact ggttcagaat
     2821 acatgattga gtgtccaaat ctagtatttg tagacaaaaa acctgttctc atttattgtc
     2881 ctcaagggtt ggataaatct gagctaaatt atgggaatat ctatccgaat acttttaaga
     2941 tctgtcaatc gcttgacata gaaaaagtaa aacttgtggg cgcttcagaa attcaaaatc
     3001 ttgattatgg atttgaagcg tatgcgactc aaggattcaa tactcctgat ggtcgtgctc
     3061 tgattgtcag ttggattgga ctacctgata tcgattatcc aactgataag tatgattatc
     3121 agggagcaat gagtcttgta aaagaacttt caataaaaga tggtaaactt taccaatatc
     3181 cagtagaagc tattacatca ttacgtgcta gttctgaaca atttacagca aaagcagaaa
     3241 caaataatac ttatgagctt gaactaactt ttcctccaaa tcagaaatcg gagttacttc
     3301 ttttctctga tagtaaagga aatggactta gcttgattgt agataccaaa gaaggaaaaa
     3361 ttattttaga tcgcagcaaa gcaggcattc aatatgccac tgactttggt acgattcgtg
     3421 aatgctcaat tgatactaaa gaaacaactg ctaacatttt tgtagataac tcaatcattg
     3481 agatttttat taataaagga gaaaaagtgt ttactagtcg tgtattccct gaaaatcacc
     3541 aaaatggtat tcaaatcaaa tcaggcgaac ctactggaaa atactttgaa ttaaaatatt
     3601 aaaatggtag caaaattaac tgatgtcgct gaattagctg gagttagccc aacaaccgtt
     3661 tcacgcgtca ttaataataa gggatattta tcagaaaaaa caaaacaaaa agttcatgaa
     3721 gctatgaaaa ctcttggtta taaaccaaat aatttagcac gtggacttca aggaaagtca
     3781 cctcagctta ttggtcttat ttttcctaat attagtaata tcttttactc tgaactcatt
     3841 gagtatctcg aaatagagtt atttaaacat ggatataaaa cgattatttg caatagtcaa
     3901 aatgatcctg ctaaagagag agaatacatt gaaatgcttg aagccaacca agttgatggc
     3961 ataatctcat caagccataa tcttggaatt gctgattatg agcgtgtgag agcacctatt
     4021 gttgcttttg atagaaattt agctcctaat gtttccattg tttcttcaga taactttgaa
     4081 gggggaaaac ttgcagctaa gactttacaa aaaaatggtt gtcaaaatac cattatgatt
     4141 acaggaaatg ataacacgga ttctcccaca gaattacgtg cactaggttt ttcttttcaa
     4201 aatccaaatg gaaaaatttt caaaattcct aataatctgt ctactatccg tcgtgaaatg
     4261 gaaataaagt caactattat aaatcacaaa ccagatggta tttttgtttc tgatgattta
     4321 acagccattc tcactatgaa aattgctcat caacttaaac ttaatatccc agaagattta
     4381 aaaattattg gatacgatgg tacctctttt attgagaaat ttttcccaca attgacaaca
     4441 ataagacaac caattgatga aattgcaagt cttattgttg atattcttct gaaaaaaatt
     4501 aaaggcgaaa agacaaataa ggattatatt ttaccaatct caatactttc tggaggaagt
     4561 atttaacgtt aaattaattt tttaatgtct ataaaattcg ttagaaaata caatttgtag
     4621 atatccaatt gatttgtaaa caaaaaggag ttgtcaactt gtagtgaccc ccaaaagtta
     4681 gacttttttg gagtggagaa atccactcct tttttcatgc tctaaaatga tatttttaca
     4741 caactgttac ggttgtttta cgaaattgga cgggactttg ccagtttaat ttctgtttta
     4801 tccgttcatt attgtagtaa tagatatagc tatcgatggc tgattttaat tcctcgaaac
     4861 agttataaac tttcccgtga aaaatctctt gttttaataa gccaaaaaaa ttctccataa
     4921 gtgaattatc taagcagttc cctttccgag acatgctttg aaagattttg tactccttca
     4981 atttgtttct ataagctttc atttgatacg cccaaccttg atctgagtga aacgtacgac
     5041 gatagggaca atcgttagtg gtctggatag cttcctccaa accttccatt accgctaatg
     5101 cattgggctt ttcagagata cgataggata aaatctcact attgaacata tccataaaag
     5161 gatctagata caatttcttt tgacggataa tgcctttggc atccacttca tggtatttaa
     5221 attcagttgt atccgtcgtg attttctgat ggcagatact ggtgtagaaa cgacgatgga
     5281 tccgattttt ggctactgct ccgacttgtc ctcgataaga attgtagcgc cgtgatttac
     5341 gtgtgtatgt cgttactttt attcctagct tcttgatcag gcgctgaact ttctttttat
     5401 tgatagagaa accgcggttt cttagttcat tggtcatccg taaacagcca taatccttgt
     5461 gcttctcccg aactttaagc atttccgctt caatctcttg atctggattt ttacggtcca
     5521 atcgtttttg ccagtacata aagattgcct tagggaactt taaagtttct agaagctcaa
     5581 ctaatttaaa cggtcctcgg aggcttgcga tgattcgtgc gattgcttca ttcgtcgttg
     5641 ttgggcttcc tgttttcgca gcttcctcaa ttcttttaaa aaggcatttt caatttgaag
     5701 ggaacggact cgtttctcca actcctcgat gcgttcacgt tctttagacg ttgctttagt
     5761 tgtagatttt ttcttatttt tgggttcatt tttcttagac aagataggtg gacatccttt
     5821 cgtttttgag agtccatcga tcccaccctc acggaattga cgcatccaac tagcgatcaa
     5881 attagggttg ttcatgttaa gtgtgttggc aacttcacga taggacagct cacttgttag
     5941 atataactct atcgcatcca gcttaaattg aacagaatat tttttctttt gtcgctttct
     6001 tactagaccc tcttcaccaa attcttgata ggcattgatc cagcgcttca cttgtgatgg
     6061 agccttaatg gaatatttct ttgccagata atcacttccg cctttaccat ccaagtaatc
     6121 atgaacgatc tttaatttaa gttcaaaagt atacttagcc ataaaaaata ccgacctcca
     6181 aaagttagat tttttggtct aacttttggg ggtcggtaca aaacttaaaa gttttagacc
     6241 cttttatttt cttaatgact tcagtcagtt ttgtgcgtaa agcgtgcgaa acaagaaaac
     6301 cgcgcgtttc tacgcgtgga agaagtatct ttagcgataa aaactccttt cgatatacta
     6361 gtgagtggct accaaccgct catcagatcg aaaggagttt tttcatgtca aatgacgata
     6421 aaagtttagc atatactcgt tggaattgta agtatcatat tatttttaca ccaaaatatc
     6481 aacgaaaagt aatctatggg caattacgaa aagatcttgg aaaaatactt aggaagttgt
     6541 gcgaaatgaa agatgtggaa atcatagaag cacatgcaat gctagatcat attcacatgc
     6601 ttgttcgagt accaccaaaa atatgtatat ccagttttat ggggtatctg aaaggtcgta
     6661 gtgcggtact aatccacgag cagcacgcaa atctcaaata taaatacgga aacaaatcct
     6721 tttggtcgaa gagatactat gtgagtacag tcggattgaa tcaaaagaca aatgaaaaat
     6781 acatccgtga acaagaagct gatgatcgca ttcgatatat tataagtaaa agagagtacg
     6841 tagaaccatt taaaaaataa agagaagtaa gtgagtgaag atgagcagtt ggcggtcttt
     6901 taaaaatccc cttttaaggg gcagcgagtg tcgacccctt ctagggggat taaaaataca
     6961 cccgtcctca cgggtgtatg ggttactgat agacttgatt ttaactggtg tcagaaactt
     7021 atgtccagaa agggtgatga aattttggca atttacggct attcaagaat tagcacaagc
     7081 aaccaagatt acaaaacaca aattcaaaca ttagaaaatg ctggcgcaga aataatcttc
     7141 ctagaaaatc actggattat cgaacacctt tggaagtatt tttgagttac gtaagtattg
     7201 atgatctgtc taacttaatt tgacaattaa gagtatattt acttcagatg gtaaggcaac
     7261 aaaataaaat acaataggca gatgaactaa taaataaaat ctgtattaaa tacagataaa
     7321 ttaatataaa aatcaaaagg atcataaact tttaacaagt gatgaaatgg gtggaaattg
     7381 attcttatag acagtttgtc tcataacatt gaagtatttt gcaattttag cattgaaaca
     7441 cttttttaat cttcaacgtt gttcttataa acaagatgat tgggaatctt ttgaattaac
     7501 actgtatagc ttagaacacc ctttatagaa aacattcgct tagcaatctt aataattcct
     7561 tgtgcccgat gtcgtttaga ttcagtaggt tcctgctcat cgatcatgga aaaaatttga
     7621 aggatcagat ccttaataaa tttatttaag agaggatgat ccattgcttc atccatactc
     7681 gataaactag tgataatgag ctacacgttt ttctttcaag ttgatcaata gcttccacga
     7741 taaaaatatc tttagataaa atcaagaacc gtaggaacta cagggatatt tggtcaataa
     7801 gagccaccgc tattagtaaa aaataagcta tcaagtatgc tctcatccca agaattgcct
     7861 acttcaatcg ttaaaaaccg ttaggtttag ctaagtgggg gtagttcact caatactttt
     7921 atcaataata gagcgcaaaa ctttttctct taattcaact gaatcaatat tattgttttg
     7981 ttcataacca tgaactaata gatcacaaat ataaagttgt gatatttttc cagctaaaga
     8041 tccaccatta agaaattctt caatggctgt ttgtaaaact aagtctgcag actttcctat
     8101 aggtgaatga atatagttag ttatagcaat aatttttgcg ccattttttt tagctatttt
     8161 caatgaatca taagtatctt tcgttttccc agataaagaa aaaatgatta ctaaatcatt
     8221 aactgttaat aaagaagcga cttgggcttg aaaatgaggg tctagtacag cttttgcttg
     8281 aactcctacg cgaagaaaca tattttctaa atcaagacta gtatttccac tagatcccac
     8341 tccaaatata tatatgtttt ttgattgagt gatcatttta atggcttcat tcaatttttc
     8401 ttcgtctaaa agcctaatag tggaatagag agcttctgtt aaactattag caatttcatc
     8461 atagtacttt acactagtcg gatgttcttt tttttgacta aagtcttcct ttgcaatttc
     8521 aatttttaaa tcagaaaaac cagaaaaacc aattttttga caaaagcgaa taatagtagc
     8581 atcgccaact tttgtgtttt tcttaatatc actcatcgta ctataaataa tagttttccc
     8641 agaatttaaa ataaaatcag cgactttttt ttcagacttc gttaaaaatg gataattttc
     8701 tttaattaaa tagatataac tcatacaaac tcctctacaa tttcaacttt tctccattat
     8761 actccaaaat aataaaaaaa actcctaaat actccatttt tatctttact tttatgaaag
     8821 cggatgctat attgtttttg gagttgttat ccaaaaataa taaaaaattg gagtgtttag
     8881 gataatggat aaaaagaggt ttttagatct tatttcaaaa ggtttgattg tttcctgtca
     8941 agcattacct ggtgaaccat tttacaaaga ggaagaaagt ttaatgcctt attttgcttt
     9001 ggctgctcaa agggctggag cagttggtat acgtgcaaat tctgttcgag atattattga
     9061 aattagaaag tgtgtatcat taccaatgat tggaattatt aaaagagatt atccaccaga
     9121 atctccattc attactgcca ctatgagaga agtagatgaa ttagtagaaa ccggtgttga
     9181 ggtaatcgct cttgattgca ctcttagaaa tcgacatgat ggactatcta taaattcttt
     9241 tattactaaa ataaaagaaa aatatcctca tcagttattt atggcagata tttctacatt
     9301 tgaagaaggc aaaaatgcat ataaagcagg aatagatttt gtaggaacta ctttaagtgg
     9361 atatactgag gaaagtaaaa aacaagatgg gccagatata gatttgatta aaaaattggt
     9421 agaagaaggg atttgtgtaa ttgctgaggg aaggattcat actccagaac aagcaaaaga
     9481 gataaaaaaa ataggagttg caggtattgt tgtgggtggt gctattactc gaccacagga
     9541 aattgcatca agatttgtta aagcattaag ttagggggag aaataaaatg tttaaaaaat
     9601 tttcacaatt aggacgcgct ttcatgttac caattgctat attaccagta gccggattac
     9661 tattgggact gggaggagcg ttaacaaatg agagtgctat aaatgcttat ccatttttag
     9721 atcaaccgtg gttgcataca attttaagta ttatgagtta tgctggaaat gcagtttttg
     9781 caaacctagc actaattttt gcaattggta ttgctgtagg acttgcaaat ggagataaag
     9841 gaacagctgg tttagctggt ggagtagctt atctagtata tactgcaact attagtgggt
     9901 ttcttgaact tttttcagca aaagatgcaa cactagatac aggtgtagtg ggatcaattg
     9961 ttgttggtgg tactgttgca tttttacaca atcgttatag aaaaattgaa ttaccacaat
    10021 tcttaggttt ttttggagga tcaagattta ttccgattgt ttctagttta gctgcaatca
    10081 ttattggttc tttcttttat ttaatttggc caccaattca aaatggttta gtagttgctg
    10141 gtgaacaaat tgcccaaatg ggtagtttag gaactttttt atatggtttt ttattgcgtt
    10201 taacaggtgc tgttggacta caccacacca tttacccttt gttctggtat acatctttag
    10261 gtggtaccga aactgtagct ggatcaacaa ttgctggagc ccaaaatatt ttctttgccc
    10321 aactagctga tccaaaccac actggtctgt ttacttatgg tactagattt tttgcaggaa
    10381 gatttgcaac aatgatgttt ggattaccag cagcatgtta tgcaatgtat cgtgcaattc
    10441 ctaagaaaaa tcgtaaaaag aatggtgggc tttattttag cggggccctt acatcatttt
    10501 taactggaat tactgaacca gttgaatata tgtttttatt tgttgctcct tggctttatg
    10561 tgattcatgc attcttagat ggattatctt tttattttgc tgatatctta aatattcgtg
    10621 tagggaattc cttttcaggt ggtttgatcg actttttatt attcggtgta cttcaaggga
    10681 atgacaaaac taattggtta aaattaattc cttttggtat tgcatgggca tttatatatt
    10741 tctttgtttt tacattcttt atcaaaaaat ttaaggtagc tattcctgga atggaagaag
    10801 aatcagaagt tcattctgaa aaagtaataa acaaaaatac atcattacat gaagagtctc
    10861 ttatcattat tgaagctctt ggtggaaaag aaaatattga gtctgttgaa gcttgtgcaa
    10921 ctcgtttgcg tgttgctgtt acgaatgggg agattattca aaaacaaaca ataaaagaca
    10981 ctggtgcaac tgctgtcttc gaagtgaagg gtggcatcca agctgttttt ggagggaaag
    11041 ctgacttatt aagtcaagaa atcaatcaaa tattaggata agataattag gaggttttta
    11101 atatgtttga aattttcaaa aagaaaaaac aagaaacttt ttatgcacct tgttccggaa
    11161 aaatcatttc catcacagaa gtaaatgatc ctgtcttttc acaaaaatta ctaggtgatg
    11221 gttttgccgt catacctgat ggttcaaaaa tttatgctcc attggctggt cgagtaacaa
    11281 atatatttcc tactaaacat gctattaata taaaaagcaa ctcaggcatt gattatttga
    11341 ttcatattgg aattgataca gttgaattac aaggagagcc tttttcgatt tgtgttcaag
    11401 aaaatgatga ggttactcct gaaacctcgt tggttgatgt ggattttgac aaaataataa
    11461 aatctggtaa agatacttct gttatagttg tttttccgga gaaaaatcag ttcaatgatt
    11521 taataataga taatggtcga attgctcatg ggaattcatg tggaaaaatc actttttaaa
    11581 aaagtgtata taaaaagcta taaaataagc taaataggat atttgtcaaa tgaagttggt
    11641 gaacagaaag aactgttttc tttctgttca tttttttatt ttattagtaa tgattcccca
    11701 tgccaaatca tgatccgtaa tttaaagtta taaaagcttc gaaatccatt ggcatttcct
    11761 tggaatacct tgatatgatt attcaaatat aaaatataga gagaagagag agagggaaga
    11821 tatttataaa gggcaaagtt atgtttactc aacttaggaa atagaaatag atcaatttat
    11881 attctcttgt caatcgaata tgaaccttag cactatatac caaatatagt aaaatgctga
    11941 aagatg
//
LOCUS       DAJSMI010000081        11939 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJSMI010000081 DAJSMI010000000
VERSION     DAJSMI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.081"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            24..266
                     /gene="yaaA"
                     /locus_tag="ON430_001726"
     CDS             24..266
                     /gene="yaaA"
                     /locus_tag="ON430_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCR3219642.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            253..1377
                     /gene="recF"
                     /locus_tag="ON430_001727"
     CDS             253..1377
                     /gene="recF"
                     /locus_tag="ON430_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR3219643.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1374..3320
                     /gene="gyrB"
                     /locus_tag="ON430_001728"
     CDS             1374..3320
                     /gene="gyrB"
                     /locus_tag="ON430_001728"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR3219644.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3340..5811
                     /gene="gyrA"
                     /locus_tag="ON430_001729"
     CDS             3340..5811
                     /gene="gyrA"
                     /locus_tag="ON430_001729"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCR3219645.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6047..6346
                     /locus_tag="ON430_001730"
     CDS             6047..6346
                     /locus_tag="ON430_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR3219646.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6396..6908
                     /gene="ssb"
                     /locus_tag="ON430_001731"
     CDS             6396..6908
                     /gene="ssb"
                     /locus_tag="ON430_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3219647.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6934..7170
                     /locus_tag="ON430_001732"
     CDS             6934..7170
                     /locus_tag="ON430_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR3219648.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7313..9286
                     /locus_tag="ON430_001733"
     CDS             7313..9286
                     /locus_tag="ON430_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCR3219649.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9292..9744
                     /gene="rplI"
                     /locus_tag="ON430_001734"
     CDS             9292..9744
                     /gene="rplI"
                     /locus_tag="ON430_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR3219650.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10018..11385
                     /gene="dnaB"
                     /locus_tag="ON430_001735"
     CDS             10018..11385
                     /gene="dnaB"
                     /locus_tag="ON430_001735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR3219651.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         3985 a         2176 c         2648 g         3130 t
ORIGIN      
        1 ataatgaaaa tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga
       61 cgttgggcca aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt
      121 atttggcaga aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac
      181 tttatcccgg catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag
      241 gaaacgacga taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag
      301 ctgaacatcg aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa
      361 acgaatcttt tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt
      421 gagcaggaat tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa
      481 ggttcatcca ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc
      541 aaccatatcg aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt
      601 gcaccagaag atctttcttt agtaaaaggc agcccgcaga tcaggcggaa gtttttagat
      661 atggaattag gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt
      721 ttgaaacaaa gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt
      781 tatttggata ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga
      841 gcacagttcg tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat
      901 caaaaagagc agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg
      961 caagagcaaa tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt
     1021 tttcggggaa caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag
     1081 aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa
     1141 ttagcagaaa tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat
     1201 gatgtgatga gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga
     1261 aaagttcaaa cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt
     1321 gaacctgaga ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag
     1381 aagaaagaag tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg
     1441 aaggacttga agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg
     1501 gtctgcatca ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt
     1561 ttgcaacgaa aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg
     1621 gtcgggggat acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct
     1681 ttactgtttt acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac
     1741 ttcacggagt aggatcttct gttgtaaacg cgctttctac acagcttgat gtcaaagtat
     1801 acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa
     1861 agattatcga acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa
     1921 tttttacaga gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg
     1981 ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca
     2041 tcaatgaata ccattatgac ggcgggatca aaagttatgt cgaatacttg aatgcaaaca
     2101 aaaccgtgat ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg
     2161 aagtctctat gcaatataca gacggctacc attccaatat catgagtttt gccaacaata
     2221 tccatacgta tgaaggtgga acacatgaat caggattcaa gactgcttta actcgtgtga
     2281 taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg
     2341 aagacgtgcg agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg
     2401 aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat
     2461 ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgg caaattgttg
     2521 aaaaaggatt actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga
     2581 gacgtaaagg tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca
     2641 acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta
     2701 agcaaggtcg taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg
     2761 ttgaaaaagc aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg
     2821 ctatgggtac agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag
     2881 ttatcatgac tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt
     2941 tttatcgtta catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt
     3001 tatacggggt aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag
     3061 aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt
     3121 ataaaggact tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca
     3181 atcggatgat ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa
     3241 tgttgatggg ggatcgagta gaaccccggc gtgcattcat cgaggaaaat gcacactatg
     3301 tgaagaactt agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa
     3361 aacatccatg atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg
     3421 agtgttattg tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc
     3481 cgtattttat acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct
     3541 gccagaatcg tcggagatgt catgggtaaa taccatcctc atggagaccg tgcgatctat
     3601 gagtcaatgg tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat
     3661 ggaaactttg gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg
     3721 atgagcaaaa tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa
     3781 agcaactatg atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt
     3841 ttagtgaacg gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac
     3901 ttgtctgagg tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat
     3961 gaattgatgg aagtattacc tggaccagat tttccaacag gtggtttggt tatggggaaa
     4021 tcaggaatcc gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt
     4081 gagattactg agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg
     4141 gtcaataaag caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa
     4201 gggatcacag atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc
     4261 cgtcgtgacg ccagcgcatc cgttatcttg aataacttat ataaattgac atccttgcaa
     4321 aattcatttg gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg
     4381 aaacaaatcc ttgaaaacta tatcgaacat caacgcgaag tcatcacacg acggacggtc
     4441 tttgaaaaag aaaaagcaga agcaagagca catatcttag aaggtttgcg tattgctttg
     4501 gatcatatcg atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa
     4561 gcaacaatga tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg
     4621 cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc
     4681 ttaaaactga ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc
     4741 atcaagaatg aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta
     4801 ctagtcggtg aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt
     4861 attacattga caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa
     4921 cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat
     4981 ctggtttctt gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat
     5041 cgggcaaaag gatacgaaat tccagaatac gggcgtacag ccaaaggaat cccaatcatc
     5101 aatttactag gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa
     5161 gcagaagaag gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct
     5221 gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac
     5281 gatgagctcg tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat
     5341 aacggctact ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc
     5401 ggggtcagag ggatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag
     5461 gaaaatcaag aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg
     5521 gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa
     5581 aatggtccac tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca
     5641 gataaaggtg tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca
     5701 ttaggcgtac gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc
     5761 gaaccagaag ctgcagaaca aacagttgaa gaacaaggat cagaagaata accatcaaga
     5821 ctaagtgtgt aagctagcaa gaataaccga ttaaaaaatc tggttattcc tgctggcttt
     5881 tttgattagt tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac
     5941 caaaatagag tgtataatta gagcttgtga gtagtctaat taattagata actctccttg
     6001 ctcttaggaa cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa
     6061 atatgaaatc atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat
     6121 cgaacgtttc gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg
     6181 ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt
     6241 taaagtatct tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat
     6301 caacgacgat atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt
     6361 gaaacaatag aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg
     6421 gacgtctgac gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat
     6481 ttactttggc tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta
     6541 tcaactgtgt gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa
     6601 cacttttagg tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac
     6661 gtgtatacgt gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat
     6721 ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg
     6781 gcggaaacaa taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc
     6841 gtgatacttc tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac
     6901 cattctaaac gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt
     6961 aaacgtcgta aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat
     7021 atcgaattac taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact
     7081 ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg
     7141 ggcttgctac ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt
     7201 ttgatcagct attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc
     7261 gaatgacgaa agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa
     7321 taaaaacaat catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt
     7381 attggtagtc ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa
     7441 tttatggggt gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga
     7501 aaaaatccag caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat
     7561 gcctgtagga gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt
     7621 agcattgaaa atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt
     7681 tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga
     7741 tatggataca gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa
     7801 actccatcag acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga
     7861 tgccattgat aaattagatg acaaagagat ttcttatttg aactctttcg tcactacact
     7921 agtttctgac tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt
     7981 tttcttcgta gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct
     8041 ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat
     8101 tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt
     8161 tgctttagtc cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc
     8221 tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc
     8281 gatgagtacc gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca
     8341 caagtttccg gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt
     8401 caaccagaaa aaagcttaca tcgttatcaa tgagaacgag atcattccag atgtagaacg
     8461 gtgtcttgaa gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg
     8521 tgccatagag ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc
     8581 tctatcgatc tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca
     8641 tcgtcgtggg gaagagtttc caagtaaacc tctgctgact tatattgaat cttcagcttc
     8701 ttcagcttca gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt
     8761 agatcgtttt gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa
     8821 tgtccgtaca actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga
     8881 cgcatcctta gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa
     8941 tttgattgct ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga
     9001 aaaagagtat aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa
     9061 tatcaatgca gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag
     9121 aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt
     9181 cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt
     9241 tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta
     9301 atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca
     9361 ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt
     9421 atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa
     9481 gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg
     9541 aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg
     9601 ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc
     9661 cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc
     9721 aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa
     9781 ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca
     9841 aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca
     9901 taaagaaaac gagtataata aaagagaact atttttttat tacactttcg gtacgaatga
     9961 tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg
    10021 aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta
    10081 ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa
    10141 gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt
    10201 aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa
    10261 gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat
    10321 atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca
    10381 gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat
    10441 gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct
    10501 attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa
    10561 gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag
    10621 cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg
    10681 aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa
    10741 atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc
    10801 catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg
    10861 agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc
    10921 cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat
    10981 tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc
    11041 tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag
    11101 ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa
    11161 tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat
    11221 gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa
    11281 gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa
    11341 gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag
    11401 ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta
    11461 gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct
    11521 ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa
    11581 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac
    11641 gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca
    11701 tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc
    11761 tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca
    11821 aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc
    11881 tttttcacaa cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcga
//
LOCUS       DAJSMI010000082        11515 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJSMI010000082 DAJSMI010000000
VERSION     DAJSMI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.082"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(79..2154)
                     /locus_tag="ON430_001736"
     CDS             complement(79..2154)
                     /locus_tag="ON430_001736"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR3219652.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="ON430_001737"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="ON430_001737"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR3219653.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="ON430_001738"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="ON430_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR3219654.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /gene="era"
                     /locus_tag="ON430_001739"
     CDS             complement(4412..5311)
                     /gene="era"
                     /locus_tag="ON430_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR3219655.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5721)
                     /locus_tag="ON430_001740"
     CDS             complement(5326..5721)
                     /locus_tag="ON430_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCR3219656.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="ON430_001741"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="ON430_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR3219657.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="ON430_001742"
     CDS             complement(6198..8390)
                     /locus_tag="ON430_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCR3219658.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="ON430_001743"
     CDS             complement(8414..9385)
                     /locus_tag="ON430_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR3219659.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="ON430_001744"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="ON430_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR3219660.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMPLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3112 a         2420 c         2104 g         3879 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcctttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga aaaaa
//
LOCUS       DAJSMI010000083        11317 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJSMI010000083 DAJSMI010000000
VERSION     DAJSMI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.083"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            179..3457
                     /locus_tag="ON430_001745"
     CDS             179..3457
                     /locus_tag="ON430_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCR3219661.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3998..5905)
                     /locus_tag="ON430_001746"
     CDS             complement(3998..5905)
                     /locus_tag="ON430_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCR3219662.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6035..6910
                     /locus_tag="ON430_001747"
     CDS             6035..6910
                     /locus_tag="ON430_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCR3219663.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6990..7964
                     /locus_tag="ON430_001748"
     CDS             6990..7964
                     /locus_tag="ON430_001748"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR3219664.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8144..9289
                     /locus_tag="ON430_001749"
     CDS             8144..9289
                     /locus_tag="ON430_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3219665.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9426..9770
                     /locus_tag="ON430_001750"
     CDS             9426..9770
                     /locus_tag="ON430_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCR3219666.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10041..>11317
                     /gene="efmA"
                     /locus_tag="ON430_001751"
     CDS             10041..>11317
                     /gene="efmA"
                     /locus_tag="ON430_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCR3219667.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQ"
BASE COUNT         3625 a         2047 c         2393 g         3252 t
ORIGIN      
        1 tattttaaaa aaacttattc tttttacgtt aataaatctc tttattatca acaacttaag
       61 ttatattttt tctttttata aaatacactt attataattt tgttttttat tgacagtaaa
      121 aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag gaatacactt
      181 gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc tcagtacccc
      241 ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga acgaagaaaa
      301 tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga
      361 tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa
      421 aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag aaagcacaag
      481 tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt ccacgaataa
      541 cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc aagcaatccc
      601 caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga cctatacgaa
      661 tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc atttgtttgg
      721 gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat atgtctggga
      781 ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc gacttggatt
      841 ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc cagctcctgg
      901 gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg aaaaagaaac
      961 aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca cgaactcgat
     1021 tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat attattacga
     1081 tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa aattatacgc
     1141 ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga cgatcaaggt
     1201 agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc aagaaagacc
     1261 tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag gaaaccaaga
     1321 tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa ataaagcaac
     1381 aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg aatttgatta
     1441 tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc cagataaaaa
     1501 ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga ttcttcatac
     1561 agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag ggtcagatgc
     1621 aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat acgcatatgc
     1681 tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat tgacgattcc
     1741 aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga aaaatccaga
     1801 aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac tgttcagtca
     1861 ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa aaggattaca
     1921 gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg gaaatacagg
     1981 aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc ataacttagt
     2041 caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg atggatttgc
     2101 ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt atcataatgc
     2161 agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat
     2221 caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag aaggtaacgg
     2281 taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga tcaatagttt
     2341 gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata ttatcgtcaa
     2401 tagcagtaca agttttaata acggttcata taatgtggct ctttatacct cttcagcagg
     2461 aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc ttgagatgag
     2521 agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt tgaactacta
     2581 ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat caaaagattg
     2641 gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa
     2701 tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc aaacagaaaa
     2761 ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag aaacctcacc
     2821 gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa aaccaaatga
     2881 acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa
     2941 tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac
     3001 gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg aaacacagtc
     3061 aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag gagaatatac
     3121 gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg ctcatacaga
     3181 atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa
     3241 tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac caaatggaaa
     3301 accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc
     3361 aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg
     3421 tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg
     3481 taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact
     3541 tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta
     3601 cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc
     3661 tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa
     3721 tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt
     3781 ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     3841 actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact
     3901 tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt
     3961 cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc actctttttt
     4021 ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa
     4081 ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta
     4141 aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga
     4201 attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc
     4261 ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag
     4321 gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt
     4381 cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa
     4441 atgcatggga aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg
     4501 attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc
     4561 gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca
     4621 ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat
     4681 ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg
     4741 ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt
     4801 cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca
     4861 tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt
     4921 agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct
     4981 gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga
     5041 attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga
     5101 ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag
     5161 ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca
     5221 gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat
     5281 ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt
     5341 gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag
     5401 ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg
     5461 tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct
     5521 tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg
     5581 gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt
     5641 gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg
     5701 caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg
     5761 tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg
     5821 gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat
     5881 cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat
     5941 tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc
     6001 aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact
     6061 gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc
     6121 cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga
     6181 agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa
     6241 atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat
     6301 cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag
     6361 gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta
     6421 taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct
     6481 tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa
     6541 tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact
     6601 tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa
     6661 tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg
     6721 tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca
     6781 ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca
     6841 cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc
     6901 acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg
     6961 aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga
     7021 agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa
     7081 cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg
     7141 aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg
     7201 attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga
     7261 tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg
     7321 aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac
     7381 tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt
     7441 tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca
     7501 tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt
     7561 tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg
     7621 tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag
     7681 caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct
     7741 gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca
     7801 aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa
     7861 caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac
     7921 cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga
     7981 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
     8041 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
     8101 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
     8161 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
     8221 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
     8281 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
     8341 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
     8401 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
     8461 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
     8521 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
     8581 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
     8641 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
     8701 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
     8761 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
     8821 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
     8881 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
     8941 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     9001 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     9061 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     9121 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga
     9181 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     9241 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     9301 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     9361 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     9421 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     9481 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     9541 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     9601 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     9661 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     9721 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     9781 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     9841 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     9901 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     9961 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
    10021 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
    10081 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
    10141 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
    10201 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
    10261 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
    10321 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
    10381 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
    10441 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
    10501 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
    10561 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
    10621 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
    10681 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
    10741 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
    10801 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
    10861 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
    10921 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
    10981 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
    11041 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
    11101 gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga ttattatttg
    11161 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
    11221 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
    11281 tacagaaaaa actggtaaaa gaacaccatg agcaaaa
//
LOCUS       DAJSMI010000084        11233 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJSMI010000084 DAJSMI010000000
VERSION     DAJSMI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.084"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            5..304
                     /locus_tag="ON430_001752"
     CDS             5..304
                     /locus_tag="ON430_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3219668.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            377..1738
                     /locus_tag="ON430_001753"
     CDS             377..1738
                     /locus_tag="ON430_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR3219669.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1760..2020
                     /locus_tag="ON430_001754"
     CDS             1760..2020
                     /locus_tag="ON430_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219670.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2034..2786
                     /locus_tag="ON430_001755"
     CDS             2034..2786
                     /locus_tag="ON430_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCR3219671.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2880..3485)
                     /locus_tag="ON430_001756"
     CDS             complement(2880..3485)
                     /locus_tag="ON430_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3219672.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3538..4512)
                     /locus_tag="ON430_001757"
     CDS             complement(3538..4512)
                     /locus_tag="ON430_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCR3219673.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4890..5609)
                     /locus_tag="ON430_001758"
     CDS             complement(4890..5609)
                     /locus_tag="ON430_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3219674.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5736..7007)
                     /gene="celB"
                     /locus_tag="ON430_001759"
     CDS             complement(5736..7007)
                     /gene="celB"
                     /locus_tag="ON430_001759"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR3219675.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7160..8557
                     /locus_tag="ON430_001760"
     CDS             7160..8557
                     /locus_tag="ON430_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3219676.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8638..9078
                     /locus_tag="ON430_001761"
     CDS             8638..9078
                     /locus_tag="ON430_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3219677.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9226..9552
                     /locus_tag="ON430_001762"
     CDS             9226..9552
                     /locus_tag="ON430_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3219678.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9553..10302
                     /locus_tag="ON430_001763"
     CDS             9553..10302
                     /locus_tag="ON430_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219679.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10382..10837)
                     /locus_tag="ON430_001764"
     CDS             complement(10382..10837)
                     /locus_tag="ON430_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR3219680.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3564 a         1961 c         2303 g         3405 t
ORIGIN      
        1 aaaaatgaaa aatcggttaa ttgtcgcatg tggaagtgga gtagcaacga gtcagacaat
       61 cgctagtaag atccaaagta tgttagaaga tgacggcatc gcatttcctg tagaagcagt
      121 tgattacaaa tcgattcaaa atgagcttct aactgctgga atctatgtgt atgtcgcaca
      181 acctgatgag gaagtacttg aacaagcaaa agatttgggt attgaagtat ttccaggaat
      241 tccattttta actggaatgg gtgtagaacc tatttatgac agtatcaaag agttagtaca
      301 gtaaaggagg attgttggat aaaaaagttg gttcatagtt ttatttacta tatttagaaa
      361 gaaagaaggt ttatttatgg aagttttcca aaccattatc aattatgttt tgaacttagg
      421 atcagccatt tttgttcccc tgattatttt gatacttgga ttgttggcag ggatgaaatt
      481 aaaaaaagca ttcatgtctg ctttgacttt agggattgct tttagcggaa tgtctatggt
      541 cattggctat atgtctaacg cagtaagtcc agcatcagaa gcattggcta aaaatacggg
      601 aatcagtttg cctgcacttg atcttgggtg gacaggtgca gcaagcatca catggtcgtg
      661 gagttatgct tttatatttt ttgcagtaac gattggggtc aattttgtac tgttactttt
      721 taattggacg aagacgttaa atgttgatat gtggaatgtt tgggggaaag ctttaactgc
      781 ttatttagtt tattatgttt ccggtagttt ggctgcaggc tttcttactg caatggtcca
      841 agttatattg gaattaaagc ttggtgatat gtttcaaaaa catatacaag atctcacagg
      901 gattccatta gtaacagtga ctcattttat gacatctgcc gctgtcttgt tgctgccttt
      961 caatatgatc atggataaaa ttcctgcttt aaataaacga gcggatacga atgcattgaa
     1021 gaagaaaatt gggatcttct ctgaaaactc tgtaatggga ttcattattg gattggggct
     1081 tggctttgct gctgcgtatg gcgtttcagg ttctcttaat ctagcgatcc aaacagctac
     1141 agcattaagt ttgtttccta tgatctctaa aatgttcatg caatctttgt ctcctctagc
     1201 agacgctatg tcagaattta tgaaaaagcg gtttaaggat cgtgaagtat atatcggatt
     1261 ggactggccg atattagctg gtcgttcaga aatttgggtt actgctattt tacttgttcc
     1321 tgtgtttatt ggttatgcca ttattttgcc aggaaaccaa gtattgccat tagcagggat
     1381 tatcaactat tcgattgcgg tcggcggatt acttttaaca ggaggaaact tggctcgtat
     1441 gctgatcttg ggaatcatca ctatgcctat ttatttgtac ggggccactt accttgcgcc
     1501 aatactcagt gatttagcag aaaaaagcgg agcagtcgag ggaatcaaga aaggacagtt
     1561 gatcacatgg tcatccatag aagggccgga gttcagggtt ttatttgcgg aggcatttaa
     1621 taggaatatt ctcgctattg taggatgcgt gatttttcta ggactgtttg ttctacttta
     1681 taaatatatg ataaaagcga aagtaccgag tgaacgttat gctgaactac aaaaatagca
     1741 ttgaaatggt gggaaagtaa tgaaaagaga agagacagat aaaataaaat ggacagtcgc
     1801 tctttgtggt acacttctgc tatttctata tggactcttt actcagaata tcatcatcaa
     1861 tctattggtc atttttttcg cactagtgat ctataaatat ggaaatcacg ttttattcag
     1921 agagtacgat gaaaaaagaa aacggaaaat agaagaatca atgaaaataa aagaagctac
     1981 caaagaaata ttgagagaaa aaagttttat aaaaagataa tggaggggtc attatgtcag
     2041 atggacgtat gttatttgag agagaacgac aggacatggc acgtatcgta agagtgattt
     2101 tcgaaaggaa aaacacaaat gtcgcaggag ggaatttttc ctttaaaacc actgatgatc
     2161 aagggaaaga ctacatcatc atgacgccga ccatgatgtc agaagcctat ctaggagaga
     2221 tttctcctgc acaggttcta gtagtagaac caagaacgcg tagaatcgtt gacggagtcg
     2281 gtcatttaac tcgtgaaatc aatatgcatg aagcgatgta tgagactaat ccagctatca
     2341 aagctgtcct acatgctcat gcgcctaatg caatgttctg ggcgactact gggttgccta
     2401 tgcctaattt gacagaagcc acagctaaag taaaagagat tcctgtcatg gactttgagc
     2461 ctaattgctc tgaagaactt gctgaattga caagcgacta tgtaaaaaaa cagcatctcg
     2521 aattaccgca tatgttgctg ctcaactctc atggcgtatt gatcaacaca ggtggagaat
     2581 caggaatcga agctatccat aaagcactgc aaatccttga tacagttgaa tggaatgcag
     2641 aaattgccta caagcagacg atttttcaag cattagggat tactgacggt ttttattcaa
     2701 aaggtgagaa aatcggggga ttggaagagt tgaaacataa aacggctatt tataataaat
     2761 ctgttattgc agctggtgga gactaaccta tctgacacaa aaaactacga ctttcacaaa
     2821 atctgatcca gatcgatatc tgcttcaggt tgtattatgt aagtcgtagt tcttttcttt
     2881 tatttagtta agaggtggtc tgtccattcc ctcgcttgct tgatttcgtc catcgtcaac
     2941 tgatgacctg ttacagttag ctgaatagtc acatttgctt ggcgtgtttc gaattgatcg
     3001 gccaattctt taaaggaagc aggggaaacg attggatcgt tatcaccata agaaatccag
     3061 acggttttat cctctaatgt ggaacggctt tgatgaacac ctagagacat cggatggaaa
     3121 aacaaccctt tatttagctc ggtctcacgc tccaataata aatgagcagc gatgtttgcg
     3181 ccatttgaat agccgacaag cagccagtca cttaacggta tcagttcttt ttcgctgatt
     3241 tctttgattt tttcatggag tttatctgtt tctttttcca aactgtccag gtcgaattga
     3301 ttcaatccat tacgcttgaa aaaacgattc atcccatctt cttggaccgt gccacgaaaa
     3361 gaaagcaatg tactgtttgg tgcaagaaag tgagcgatat ccagtaaaga atgttcatcg
     3421 ccacctgtgc catgaagtaa gatcaaatgc ttgccacttg ttcctttttc aaaaatgtag
     3481 gtcattttgt tccctctttt ctgtttctat agatgcaaat gaagtagcat gaagagatta
     3541 ttcacgtttt ttatttgcat cacttgtatc gaaaggacgg acataagctt caatggcttc
     3601 acgacgttct tctaagaatg gaggaagaga tagtttttct cctgctgatt ctgctggttc
     3661 atcttcaagg aatccaggac catcagttgc taattcaaac agtacgtgat gagcggctag
     3721 gaagtactca gattggaaat agaaacgatc gacgaaacca gaatttggca attcaatctt
     3781 attgattact tgaatccagt attccaatgc ttctttgtct gctacacgca atgctaagtg
     3841 atggatattt ccaaatcctt cgattgcttt tggcaagtct gtccgatgtt caataatgat
     3901 gcttgcacca tttccccctt caccgatttc aaataagtag aaatctcctg aatggtctgt
     3961 ttctttgaat cctaagattt tttcaagaat caagcggata tgctctaatt gactgacacg
     4021 gacaaagaca ggtccaagtc ctgtgatcgc aaactctgtc ggtacattgc tgtttttcca
     4081 aggaatgcca gctgctacac cttgattgtt ttcgtcagag atcaattgat aatactgatt
     4141 atcgaaatct tcaaattcga tatatttttt gccgaaacgt tcattgattg ctgaatgagt
     4201 aacgtcatat ttttcaaaac gttccaacca aaagtctaat gctgcgtcac ttggcacacg
     4261 gaaagaagca cgagagatcg tatctgttcc tttttttcct tttggctgat ttggaaaatc
     4321 aaagaaagtc atatctgttc ctggcgcacc agtatcgtct gtaaagtaaa gatggtaagt
     4381 ttcgatgtca tcttggttaa ctgttttttt gattaagcgc attcctaaaa tatctgtaaa
     4441 aaagcggtag atttttgtag cgctagatgt cattgctgtg acgtggtgaa ttcctctgat
     4501 ttgtgtatcc atgataaatt tcctcctgtt aacttacaaa aagtaagtag ttgatttaga
     4561 catagtatag agggtgcatc tctattttgc aattaatttg tcttctaaag tatcaggaag
     4621 gaatcaagaa tggaaaagag gagggacgga tcaataaaat aaaaagactt acgactagct
     4681 gtatcgattt caaaaaaact agctttaaaa agctaacttt agaatcatta cgggtggtca
     4741 taagtctttt tatgctgagt tattttttca tttttgagct aatgcttagg atagaataga
     4801 atgttgattt cacgatctct tcttgttact tgaaactgct taccactgtc acgatctctt
     4861 ggtaaacggc tttttcacaa cctctttggt taagttgaac gcacatccag cacagaatag
     4921 ttccgaacat caaatcggtt tcggctggaa gaatattcaa tcggctgtcc atctttgaga
     4981 tagacgactt gctgaacttc cagtacagga tcgttttctg cacagttcaa atattgctga
     5041 tcatattcat cacttttatc tgcagaaata gcacgataag cccctgcaaa atggacattc
     5101 aattcttgat gaagatgatc atagatagag gctaacaaat gttctttttt taaacccggc
     5161 acaagatgga ctggcataaa ggtatgctct aaaacatatg gctcgccatc taagatcctt
     5221 aaccggtgaa tattgtagac aggttgctcg gcatcgatca tcaatcgttc ttggatatat
     5281 tgattaggga attcaacatc aaaatgaatg atttgactct tcaaagaacg atgtctgcgt
     5341 ttcatttgga gactcaaacc ttcatactcg ttagcgggcg atgtgtcttt ttgcataaat
     5401 ggatgattca agattttcgt tcctgatcct cgctgcgaat agacaagtcc ttccattgct
     5461 aagatattga tggctttttt gattgtcatc cggctcgcac caaattcttt taccaagtcg
     5521 acttgattag gcaaaaaact atcgagagga tactcttttg aacggatacg ttctcttaaa
     5581 atatttgcga tttcttcgta ttttggcacg ttattcacct cttctagagt ataacgaaag
     5641 attatgcttc cgacaactcg aagtagcatc tggaacattg tttattcgaa aaaagaggtc
     5701 atgacaagtt tttgtcacaa ccccttaatg atctattaag caatatctaa atctggttgt
     5761 ttatctagta ctttcaggaa tgggtaccag ataaaggcaa cgatgacaaa gtcgatgact
     5821 tgcagcatac caccaagcca tgaatttgtt gctaagacac cgcttgcaat gattggcaca
     5881 gtccatggaa cagagacgcc agttggtgca gggacaagtc cagcagccat taccaaataa
     5941 ttgaacgtcg ttacgattag aggtgccaca acccaaggaa taaggattgt tgcattcaat
     6001 acgatcggta gaccgaagat cgctggttca tttacattga aaataccagg tgctaaagct
     6061 aatcgtccaa catcacgata ctgtttcttt ttaagaacaa aggccatcaa taaaacgaca
     6121 gctaaagtca ttccagagcc accaatacca acagtgaagg tttccatgaa tggttttgtg
     6181 atgatgtgtg gtaattcttt tccatgttga taagcttcta agttatcaag catcagtgta
     6241 ttccaaatcg ggtccattac agagttgacg atgatttgtc cgtgaagtcc gaagaaccat
     6301 aagaattgga caaagaacaa agcaatcaat gttgcaggta aactacttcc caaaccagtt
     6361 aatggttttt ggatcacttc ataaacgacg tcatgtaagt tagcattgaa aataccagtc
     6421 atcacagcat tgattactaa gaaaatagtt agtgtcacta cagcagggat caaagcggca
     6481 aatgattttg ttactgcagg cggaactcca tcaggcattt tgatctgcca gccgcgtttc
     6541 gtgattctgc agtagatttc tccagccaag aaagcagcga tcattccaat gaacattcct
     6601 tttgcgccaa gacgatcaag tgaaagcaca ccagtcacat cttcaccgcc agcggttttc
     6661 attgcaaaag gcgtaagaat caagaagctg gcaaaagaca cggcaccacc gaaaatacct
     6721 tcaacgtcat aagattttga taaataatag ccgataccaa aagtaacaaa aaccgacatg
     6781 atgctcattg ttgcattttg cccattaccg aataaattcg ataaagttcc ctttgttgcg
     6841 tcactgaaaa aaggcaagtt attgataacg acaacgatcg aaccgaacat cgttaaaggg
     6901 aaggaaagca tgaaagcatc ccgcagcact gtaaggtaac gattttgccc taataaatta
     6961 gctaagggca tgattttctc agaaagcttg tccataaaac cgttcatttt tattcacctc
     7021 aaatttattt gcaatcgatt acatacaaag tataacgcca aaaaatttaa atgtctagtc
     7081 attttcataa aaagtattga tttatttttt agggttcgtt atgattacgg tatcaagaaa
     7141 aaagagagga agtaaaaaaa tgaaaaccta cacattccca gaaaattttt ggtggggttc
     7201 tgctgctagc ggaccgcaaa cagaaggaag agtaagtgat gatggtaaag gagaaaatat
     7261 ttgggatcat tggtatgaat tagagcctga aaaattcttc gatcaagtag gacctgaaaa
     7321 aacttcgcaa gtttacacaa aatacaaaga agatgttcag ttaatgaaag aaactggcca
     7381 taacacattt cgtacatcta tccaatggag tcatttgatt ccagaaggga caggtgaaat
     7441 caatcaaaaa gctgtttctt tttatcgaga ttattttact gaattgctgg ataatggtat
     7501 tgaacctttt gtgaaccttt accatttcga tatgccacta ccactgcaag aaaaaggcgg
     7561 atggttatca agagaaacgg tcgacgctta tgtttcttat gcagcagcgt gctttgaact
     7621 gtttggagat ctggtcaaaa aatggtttac tcataatgaa ccaatcgtac ctgtagaagg
     7681 aggctacttg tatcaattcc actatccaaa tcatatcaat atgaaagaag cggtccaagt
     7741 aggctatcat gagaatctag caagcgcaaa ggtaatcaaa gcctaccatg aaggcggaca
     7801 tgatggtgag atcggaatca tcttgaatct gacaccaagc tatccgagag atgaaacgaa
     7861 cccagaagat gtaaaagcgg cgaagatcgc tgacgcattc ttcaaccgat cattccttga
     7921 ccctgcagtg aaaggagagt ttccacaaga gttgatcgaa attgtcaaag aattggctat
     7981 aatgccagag atccatgaag gagacctaga actcatccgt gaaaacacga ttgacctgct
     8041 aggaatcaat tactatcagc ctcgtcgaat caaagcaaaa gaaactccaa tcaatcacga
     8101 acatagcccc atgccagatg attattttga taattacgac atgcccaata aaaaaatgaa
     8161 tccatatcgt ggatgggaaa tttacgaaaa aggaatctat gatattttaa cgaatacaag
     8221 agaaaactat ggtaatatca aatgctatat ttctgaaaat gggatggggg tcgaaggaga
     8281 agaacgattt gttaacagcg aaggaattat tgaagatgat taccggatcg agtttgttac
     8341 ggatcatttg aaatacgtcc atcaagcaat ccaagaagga accaattgtg tgggttacca
     8401 catgtggaca tgtatggaca actggtcttg gacaaatgct tataagaatc gctatggctt
     8461 tatcgctgtt gatttggcac aagacggtaa acggacgatc aaaaaatcag gtcgctggtt
     8521 taaaacggtc tctgaaaaaa acggatttga tgcctgatat tttttgataa aacgttcaca
     8581 aaatgtttgt tctaacactg taatcgtgct acactaacag tgaaggaggc gtttacaatg
     8641 gtagatactt atcgtaatat tttagtagca attgatggtt ctgaaaaggc agaaaaagca
     8701 tttcttgagg caatcacgat tgccaaacga aatcaggcaa cgttgcacat cctgtatgtc
     8761 aatgaagtaa caggcaatta ctttggtgat tttgcatttg ttacaacaag ccttcaggaa
     8821 gaattggatg aggttgcgga aaaccagatg aaagaacatc gaaacttggc aattgaaaaa
     8881 ggcctgacag atatcgagac atatgttctt tacggttatc caaagacact gattgctaat
     8941 ttccatgaat cgaaagaacc aatcgatctg attgtcatgg gggcgacagg tctgaatgcg
     9001 gtagaacgtg cactagtagg ttccactact tcctatgtag tcaaccacgc atcatgtaac
     9061 gtcatggttg taaaataaat aaattaaggg aacagaaagc aaaaaagcat aacgcttctt
     9121 ttgctttctg ttccttttat ttttttgtaa aaaattactt tacctgatga agtaatcatg
     9181 ctattctaat tacaacgaca gatggagtaa tatggaggaa agaaaatgat ttcaagtgat
     9241 ggaatacgcg gatacaatga tgcgattatt ttatcgatcc ttaacaaaaa cgattcttat
     9301 ggatatgaaa tttctaaaac gattcgtgag aggacaaatg attcctatgt gatcaaagag
     9361 acaacacttt actccgtttt tgcccgttta gaaaagaatc aactgattgt ttcgtatcct
     9421 ggagaagtga ctcatggaaa aaagcggaca tattataaaa tcactgaaat agggcgtaat
     9481 cttttagaag aaaaaaagca agagtggctg acgacgaaag acgtagtcga tcaatttttg
     9541 gagggaaatt aaatggatac aatccgtaat tatttagaca gtctttttat cggagtacct
     9601 caaagtaccg agatagataa gctgaaaact gatttgttgg caaatatgga agaccattat
     9661 catgaattga tgggagaagg aaaaaatgaa caggaggcca tcggtacagt aatttcgaca
     9721 tttggttcaa tcgatgaatt attagaagaa ctagatgtcg aaaaaaaaca tcaagctgac
     9781 gagacggaaa caaacacagc ttctatttat ttaagcgaag cagaaaacta ctggaaagaa
     9841 tacagagcgg caagtctcca agtggcatca ggtgttctat tcattagctt atcttttgca
     9901 agttttcttt tcttttgttc agcaggatat gtgttcatgg gtatttcttg tctgattttc
     9961 ggtatagcat tagctgtcgg tttttttatt gcttctggta tgaaaatcac acgtttaaat
    10021 cactttttgc accatcggaa aatacctgaa aaagtattag cagaagcaaa agaaaaagaa
    10081 gaagagtatc agcgaagttt tggcttctca ctgattgcag gaattggatt atgtattttt
    10141 tcacttttcc cacttttggc ttctttaatg tggtatatgg atggtagtat cggtgcctct
    10201 atattttttg ttacggtagg aacgggtgtc ttcttgatca tttatggcag tctagtccgt
    10261 cattcttatc gtcaattcac tcaaagcgct tattactggt gaatgtattc agatttgtaa
    10321 aaatattcta tttatccggc gtagaaaaaa agactgtgac acgcttttgt cacagtcttt
    10381 tttatatata ataatttaaa aattcatcat ctaaatccgt agaattttct agattgctta
    10441 cccatgtaaa atcgttatag acagatttca gttgacttct gcgccattgt acagggataa
    10501 aaagacgcat gctatcttca ttgaatccga ctaataattt ctttccgtca gtaatgatgg
    10561 gacgttgtaa caaagtaaaa tgcgagacga tcagatcgac aagctctgag gtcgtacatt
    10621 ggtcgatttg gtctttcaaa tttttataat caatcgagcg tttagctaat agatcgtcaa
    10681 atccgttttc tgttaatgac aataaataaa atatctcttt ccttgttaaa ggctgtttag
    10741 gtgtgaaaat tcgttctctg taaggcattt gatggtcttt cagccagttt gccatcagat
    10801 ggtaactatg tgtgcgagaa gaatagatcg taagcataaa aaaactcctt aaatagtcca
    10861 ttaatttgtt agatctgttg agataaataa taggtaagga gctgtgcata agcattgatc
    10921 aaaacaaccc aaacatcatt agaaataact ttctaaagtc ttaaacttgc gacgagaaac
    10981 aactagacta atagaaaaaa tgtcttagcc atttggcttc ctgtttgttt ttttgcttat
    11041 gctgcagctc ctcgggaaag ggttctaaca atttgaagaa ggttgatgat tgatctagtt
    11101 taaattgtgt gaaatttgaa atctggtgtt tttactagat gaatcaccta acaactatga
    11161 ttataactta tagaacttct gttttgcatt cggttttgtt atagttgtta taaagtttta
    11221 ttttattaca gaa
//
LOCUS       DAJSMI010000085        11089 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJSMI010000085 DAJSMI010000000
VERSION     DAJSMI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.085"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            51..1310
                     /gene="lysA"
                     /locus_tag="ON430_001765"
     CDS             51..1310
                     /gene="lysA"
                     /locus_tag="ON430_001765"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR3219681.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1297..2097
                     /gene="dapF"
                     /locus_tag="ON430_001766"
     CDS             1297..2097
                     /gene="dapF"
                     /locus_tag="ON430_001766"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR3219682.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2100..2981
                     /locus_tag="ON430_001767"
     CDS             2100..2981
                     /locus_tag="ON430_001767"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR3219683.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2978..3694
                     /locus_tag="ON430_001768"
     CDS             2978..3694
                     /locus_tag="ON430_001768"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR3219684.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3700..4395
                     /gene="dapD"
                     /locus_tag="ON430_001769"
     CDS             3700..4395
                     /gene="dapD"
                     /locus_tag="ON430_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCR3219685.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4411..5412
                     /locus_tag="ON430_001770"
     CDS             4411..5412
                     /locus_tag="ON430_001770"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR3219686.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5409..6770
                     /locus_tag="ON430_001771"
     CDS             5409..6770
                     /locus_tag="ON430_001771"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCR3219687.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6730..7881
                     /locus_tag="ON430_001772"
     CDS             6730..7881
                     /locus_tag="ON430_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCR3219688.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLYKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7856..9025
                     /locus_tag="ON430_001773"
     CDS             7856..9025
                     /locus_tag="ON430_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3219689.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9200..10579
                     /locus_tag="ON430_001774"
     CDS             9200..10579
                     /locus_tag="ON430_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3219690.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10860..11089
                     /locus_tag="ON430_001775"
                     /pseudo
     CDS             10860..11089
                     /locus_tag="ON430_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3688 a         1940 c         2492 g         2969 t
ORIGIN      
        1 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
       61 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
      121 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
      181 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
      241 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
      301 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
      361 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
      421 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
      481 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
      541 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
      601 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
      661 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
      721 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
      781 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
      841 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
      901 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
      961 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     1021 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     1081 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     1141 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     1201 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     1261 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     1321 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     1381 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     1441 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     1501 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     1561 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     1621 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     1681 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     1741 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     1801 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     1861 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     1921 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     1981 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     2041 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     2101 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     2161 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     2221 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     2281 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     2341 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     2401 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     2461 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     2521 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     2581 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     2641 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     2701 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     2761 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     2821 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     2881 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     2941 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     3001 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     3061 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     3121 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     3181 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     3241 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     3301 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     3361 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     3421 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     3481 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     3541 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     3601 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     3661 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     3721 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     3781 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     3841 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     3901 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     3961 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     4021 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     4081 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     4141 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     4201 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     4261 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     4321 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     4381 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     4441 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     4501 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     4561 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     4621 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     4681 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     4741 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     4801 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     4861 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     4921 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     4981 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     5041 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     5101 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     5161 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     5221 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     5281 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     5341 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     5401 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     5461 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     5521 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     5581 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     5641 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     5701 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     5761 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     5821 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     5881 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     5941 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     6001 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     6061 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     6121 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     6181 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     6241 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     6301 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     6361 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     6421 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     6481 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     6541 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     6601 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     6661 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     6721 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     6781 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     6841 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     6901 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     6961 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     7021 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     7081 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     7141 tataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     7201 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     7261 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     7321 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     7381 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     7441 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     7501 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     7561 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     7621 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
     7681 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
     7741 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
     7801 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
     7861 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
     7921 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
     7981 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
     8041 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
     8101 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
     8161 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
     8221 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
     8281 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
     8341 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
     8401 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
     8461 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
     8521 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
     8581 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
     8641 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
     8701 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
     8761 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
     8821 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
     8881 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
     8941 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
     9001 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
     9061 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
     9121 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
     9181 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
     9241 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
     9301 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
     9361 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
     9421 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
     9481 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
     9541 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
     9601 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
     9661 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
     9721 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
     9781 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
     9841 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
     9901 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
     9961 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    10021 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    10081 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    10141 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    10201 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    10261 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    10321 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    10381 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    10441 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    10501 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    10561 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    10621 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    10681 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    10741 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    10801 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    10861 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    10921 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    10981 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    11041 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAJSMI010000086        10939 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJSMI010000086 DAJSMI010000000
VERSION     DAJSMI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.086"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            794..1267
                     /locus_tag="ON430_001776"
     CDS             794..1267
                     /locus_tag="ON430_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCR3219691.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1276..1503
                     /locus_tag="ON430_001777"
     CDS             1276..1503
                     /locus_tag="ON430_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3219692.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2139..2510
                     /locus_tag="ON430_001778"
     CDS             2139..2510
                     /locus_tag="ON430_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219693.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2766..3008)
                     /locus_tag="ON430_001779"
     CDS             complement(2766..3008)
                     /locus_tag="ON430_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3219694.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3040..3942)
                     /locus_tag="ON430_001780"
     CDS             complement(3040..3942)
                     /locus_tag="ON430_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3219695.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3955..4143)
                     /locus_tag="ON430_001781"
     CDS             complement(3955..4143)
                     /locus_tag="ON430_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR3219696.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4157..4720)
                     /gene="amaP"
                     /locus_tag="ON430_001782"
     CDS             complement(4157..4720)
                     /gene="amaP"
                     /locus_tag="ON430_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR3219697.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4758..5651)
                     /locus_tag="ON430_001783"
     CDS             complement(4758..5651)
                     /locus_tag="ON430_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3219698.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5729..6652)
                     /locus_tag="ON430_001784"
     CDS             complement(5729..6652)
                     /locus_tag="ON430_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3219699.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6701..7051)
                     /locus_tag="ON430_001785"
     CDS             complement(6701..7051)
                     /locus_tag="ON430_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3219700.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7084..7986)
                     /locus_tag="ON430_001786"
     CDS             complement(7084..7986)
                     /locus_tag="ON430_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR3219701.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7979..8836)
                     /locus_tag="ON430_001787"
     CDS             complement(7979..8836)
                     /locus_tag="ON430_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR3219702.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9170..9979
                     /locus_tag="ON430_001788"
     CDS             9170..9979
                     /locus_tag="ON430_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3219703.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10019..10516
                     /locus_tag="ON430_001789"
     CDS             10019..10516
                     /locus_tag="ON430_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3219704.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         3192 a         2057 c         1848 g         3842 t
ORIGIN      
        1 ttcattgatc ataacgattg gctatttgat tgtatcaaat ctaggaaata tggtatttgg
       61 tatcagtaaa gaattcagtt ggactactac attatgggaa tctctattct ttttcatctt
      121 cgtatttttg ttacaaaatt atcgaaaaaa ataaacatgt gccatctatc tgttggcaca
      181 tgttttagga tttatggtac tttttattcg gcaatccatt ttatttttct acttcttctt
      241 aatacctcta acacttttga tttataaact aatccaatgc atctacttgc gcttattaag
      301 ctatttcagc tagatagtac caccccatgc gtcaatgctt gattttcctt ttgtataaca
      361 ttgtataagc aataaaggac aaataagtgg tgattacacc aaaccattga ataagaaaaa
      421 ttctgatacg taaataaaca tgaacttgaa aaaattaatg aatcggttag ctactacgta
      481 gttgtctttt ttctcataca gttctgttaa cttacacttc tcttcgctag ataataaaaa
      541 atcctccaac tgtattgtcc aatataatcc caggcatcta aactagcatt atagaagaga
      601 cacacataga atctaaaata actggtttta aatgtttctt acaatcaaaa aaagctgtta
      661 aatgcactcc acaccttcat actctatcat tttttataaa cgatatattt ttattgtttt
      721 tcgataaaaa atagttgttt tacaataatt attctgctat attttagcta tcaaagataa
      781 aagagggata aaaatgaaat ttaaaacatt gctattaaaa tttgtcgttt taggtataag
      841 tgccgtattt attttgttcg gcgtactttt ttatactcag atcgctacaa gcgaaaaagg
      901 attcaatttt gattggcaga ccacattatt tgtttctgtc atttactttg cccttctcct
      961 agggatcgtt attgcttatt tcttattgaa actattgaaa aaaatcgaag ataaccaagc
     1021 tttttccact caaagcttaa acatactaaa aaaaatcgaa cgaaccattt tgtttattgg
     1081 tttcgccttt tttggcttac ttcctaaatt tatgaaagta gcggatttag acgatgctcc
     1141 aggtttgatg ttggtaggaa tcgctgtcgt attcattcct tttgcagtat acacattaac
     1201 aagcgtgctg gaaaaactgc ttgaacatgc gattttactt aagacagatc atgatttgac
     1261 agtgtgaggt gcgacatggc aattatcgtt aatttagatg tagtattagc tcaaaaaaaa
     1321 atgagtgtga ctgaattgag tgagaaagtg ggcattacga tggcaaacat ctctattcta
     1381 aaaaatggaa aagcaaaagc catccgtttt tccactctcg aaaaaatctg ccaagttttg
     1441 gagtgtcaac caggtgattt gttgacctat aaaaatgact cgaatactat accagaagaa
     1501 taaaataaag tagaacaaac agtaaataaa aaataatctg aatgatagat gtgtgagact
     1561 tttagaagtt atttacgtct attattcaga ttttttattt cgattgaata ccgttttatc
     1621 tataaataag atatgtactg actcatacaa agggggcctt ttcttctcca tgtactatca
     1681 cttttcctga tacaactggt ttcctgttac gttgtagttg gatctgcttt tagtataata
     1741 ctttattcat tccaccagac tgatagcagt ttgagcagtc taaataatca tgtagtatta
     1801 caggccattc tgtttcatac cttgaagtgc ttaaaacttc agctaatggc ccattgtgtt
     1861 aactcttact ccattctttt ttagtaagct cccatatata ctgtaaagcc ttctatatat
     1921 tatgtcatgt tgctttttaa catttactat gtagttttga taaaatgatt tgaataacag
     1981 tgatggacaa ttctttttta tttttaacat tattttgata ataatgatta tttatttaaa
     2041 aatacattag ataacatttg ttacgacacg aaaaagcttc acaaacaaat atcgttattt
     2101 taaaatgagg gcgaactcac acaattagga ggaatagtat gccatttatt gtttactttt
     2161 ttctttcatt actcgtgatc tatattccat taccaacatt tttcctgctc gtcaatcgtc
     2221 tagcagagca acatgatacg attgctgctc ttacgaaact ttgtttactt ctatttgttt
     2281 gtctagatta ctttacgagc tattattggc taaaacagac aagagtaaaa aagagatatt
     2341 atttcttcct tgtctttttc aatctctttg agtttctgat ccattgcttt ttacttcatc
     2401 agtatcatac gatccatttg tggatcatct gggttttcaa agtggtcgta ttattttgta
     2461 tgttcacctt tcctatctca agaaaattta gggaggcatt gttttattaa agaagcttct
     2521 ctgtatcaat aaagacacat ttaaggaaaa acttttaaaa atctaattgg gaatttgccc
     2581 gcataaataa gaggctgtga caaaagtctt gtcacagcct cttattccgt ataaacagtg
     2641 ttcaaaagct tgcatcttcg ataatatgcc caaccaaatg aaaaatatga gaagctattt
     2701 ttgtttggct gttcttatta cttgatgaag aacagctttt tcacgacctc tttttaaaag
     2761 tctttttaca gttttcgagc aaagaatgat acgattagta ctaaaatgat ggcaccaatc
     2821 aatgatggca cgattgccat tcctgcaata cttggtcccc aagaacctaa taatgcttga
     2881 ccaagtgctg atccaactaa acctgctacg acatttgcaa taatccccat cgaagaacct
     2941 ttattcgtga ttgctcctgc aattgctccg ataattcctc cgacgataag tgaccaaata
     3001 aatcccatgt ttcgttcctc ctaatgtttt acttccttat tactgaaccc gtggttctgt
     3061 attttctttt actttttgtg cgccgcttcc agctgctgat tttactttat ctgtttgatt
     3121 cgaagcaaat tcaccggttt ttttcgctgc gtcagttact ttgtcttgca ccgtttcttg
     3181 atctttttca aattcttctt ttgttttgat gtccactacg ttagcgttga cttcgatcac
     3241 ttctaaatgt gtcatatttt ttacttcttt gccgattact tcttgcattt cttcaaagat
     3301 tttttcgata tctttgccat attccacaat gacatccata tcaactgcta cttgtttttt
     3361 accgacttct gtttcgatcc cagccgttac attatctgta ttcacaagtt ttcctgccac
     3421 attagagaag aaaccaccgt ttgcagataa taaaccgtct acttgttcaa ttgcgattcc
     3481 gatgattttt tgcacaactt tatcttcata attcagctca ccgcgcattg aatcttcctg
     3541 ttcagaatca tgctttcctt ggttatttgt atgatcagaa ccaagttttc cttgtccgtt
     3601 tgtgtggtca gaactaagtt tttcttgtgc tttcgcatgg tcagaaggat gtttttgctc
     3661 ttccacaggt ttagaaggat cgattgcatc atgttgttct ggaactttag attctggagt
     3721 tcctggatga tgaggtgcat gcttttctgc atcatgtttc ttctcttcta ctccttgttg
     3781 aacgttaggg actactggat ctggatgtgc agatactggt gctgttttgt tcttttcatc
     3841 gttcacacca tttgattggt ttggtgtcgt tgggtctgga tgtgcagaca ctggtttttg
     3901 gttgttcatt ttattgttat tatttttgtt attgttattc atggatgttt cctcctaaga
     3961 tttaaaatag tgatctaaga ttttcgtttg tttcagataa aaaccgatag cgacgccgat
     4021 cgctgccatg atcaagatca ataaggtctt gaaaaagccc acggtgatcc ataaaacggc
     4081 taatatcaat ccaagtaccc caaagatgat agggagcttg tatgtcgaaa atagttcttt
     4141 catttttggt ccccccctat tctactcgtt tgcggtcatt gcttcgttta gctggttctt
     4201 caaagcctgt gtatttcacc gatactttca ctggctcttt tgttcccaat atgtgttgta
     4261 cttcattttc gatttctttc atcaatgttc ctgttttacc aatgagtgct gaagttcgtt
     4321 tcaactcccc ttttacattg acggcgatct tgtttttcgt tgctttgaca tttacttttg
     4381 gtgaatggat gaattcatct tcatgcaagt gagaacgaac cattccttca attgcttttt
     4441 tatccaacgt caactcccca tcaccatgtt tcagtagaaa gtttccgatt gtttttggat
     4501 aaaacaggaa aacaagtaac gtgataattg tcaaaatagc aaatgcaacg gcaagccaaa
     4561 atacatattg ttgcacgtat gaattagtga tggaaaagaa tcgaaacttc tctagctgaa
     4621 aaggcaatgg ataggttgca cttaatagta tcgcaaacaa aagcacagat agcaaaagta
     4681 atgttccgat cactccgatc gtttttacac ctttattcat tttcgcacct cctcataggg
     4741 gtgaaacgat caacttctta taagttgatc gcttctccac cagttactcc gtaaatttgg
     4801 gctgtaacat aacttgcttt attcgatgct aagaaaacat aaactggtgc taattctcct
     4861 ggctgacctg cacgttctag taaactttgc tgaccaaatt cagggatcga atcagaaggt
     4921 tgtccttcgt ctagttgtaa tggtgtccag attggaccag gtgctacacc atttacacgg
     4981 attcctttag gtgcaagctg ttttgctaaa ccgatcgtaa agttagcgat tgaagctttt
     5041 gttgctgcgt aatctaataa atgttcgctt ggattgaatg cttgaacaga agttgttgtt
     5101 acgattgctc ctcctgctgg gatatgcgga acagcttctt tcactaaagc aaacatagcg
     5161 atgatgttta tcataaatgt gtctttgact tgctcaagtg gtaactcagc aatagactga
     5221 caagaaattt gctgtcctgc gtttagtacc atcgtatcta gcccgccaag ttcactcacg
     5281 gctttatcta tgatttcttt aggtgcttgt tcgtcacgaa gatcaaaagg tagtaagaca
     5341 atcttacgtc ctgcatcttt cacgtatttt gctacttcct ctgcatcttt ttcttctcct
     5401 gggaagaatt gaagagcgat atctgcccct tctctagcaa atgcaatcgc tgctgcacga
     5461 ccgatccctg aatctccacc agtgattagt acacggcggt tttctagttg attattgcct
     5521 tcataggatt cttctccaca atcaggaaca ggaatcatat tctgctgtaa agctggtgta
     5581 tcctgatctt gtttaggaaa tttctccgta tggtacaagt ttcgcgggtc gattaatttt
     5641 ggttctgtca ttgctgtatt cctcctagtt tttaaattgt attgccaaaa caaatttctt
     5701 tgtttgatac ctgttttaaa tagattcttt aatttgtata atctttccgg tataaatggt
     5761 aacctaaaac tactgctgct atcaaagagc caaagactac gagtataaag cctagagcag
     5821 aacttttacc cggtaatcct cctgtattca ttccccaaat ccctgcgaac aacgccggaa
     5881 cggaaatgat taatgcagct gaatctagat atttcatgat atgatttaga ttattgtcca
     5941 tcatcccgtt gaatagatca ccaatactct ctaataaatc tcgatagatc tcgatcattt
     6001 tttctgtttg tcgctgtctc aaatggacat catagagtag ctgttcatcg tctaaacgtt
     6061 tgatgaaagt ttcagaattc cataattgat ccattgtttc ccgttgatct cttaaggtat
     6121 gatcgatata tacgatgtct ctttcaagat ctgcctgttt atacagttct tcattttccg
     6181 ttgttttacg agctgcttta tccaaagcgt caattcgctt tttcatctct cttagttctt
     6241 tcacgtaatg ggtgtaaata tctaataatg tatacgtgat gatcttttca aacgagttta
     6301 aatgatttcc atgctttgtc agcagccgat ccacaaattc ggtttttcta tcgatacaag
     6361 taatcaagag attatcggaa agtacaaaag atattggcgt taattttttt tcgatacgat
     6421 tatctgactg atctagatta atcactacca aagaaaatgg atgctctagt ttcgtctcat
     6481 aaaggcgctc catccgagaa acttcttccg gatattcggt tcctacgaaa atatcatcag
     6541 gaagctcgaa ttcttcgatt gcttgctcaa tctcttcggg ctttgcacta tcaagtacga
     6601 gccaattata ttttttttca tctgtttcta ttacattctt ttcttcatcc acaaagtagt
     6661 atctcaatgt atacctcctt tttggacttt ctcgaaacta ttagctgaag aagcagttct
     6721 ttaatttgat attataatcg agtactgcga tttcattttc taatgaaaag ctgtcaagct
     6781 gtttgttgaa gtccatgttc aatcgtttac cgaaataatt ttgctctaat ttagatgtat
     6841 agatgtccaa taatggatgc tcgattttag tcgcatattc tccaaattcc gaaacaaaag
     6901 gaatgatttg aaaaaagctg ccttttgcaa aagcaccttt gtcccttaga aatggatcgg
     6961 aatcattgat aagcgcgagt gcgatttttg tattttcccc aaccttttca attaacgtct
     7021 ctactgcatt tttagacatt gtaatttcca ttcctactcc ccctcttcag ttgttgtctt
     7081 ctattattta acaactacat ctgtttttgg ttgaactttt gcatattttg gatatttagg
     7141 gaatttagct acacaacgga aagccgtcat ccctgcatat ttttcgtaat atttcttcgt
     7201 tggcccttca aaatccttgc tccacggttt cacatgtcct gaaaaatgga taatgtaagg
     7261 attgttgcga gtttcttcgt attcacgttc acctgctgct gttggatgtt tttttgcttt
     7321 tgccatgata tagccttgtg cattccaacg cggatgaagt ggtaaccaac ggtcatgtaa
     7381 gatagcattt aatgcatctt ggtcatggaa tcgtaatttt tctggattat gttcgatgaa
     7441 atctaatact ttttgcgtaa tattttcatc taaccatttt ttcacattga tcagcatcaa
     7501 acctgagttg aaataacgca ttgatttagc tgaaattttc attttttcca gtcgttgatg
     7561 gaacccagcg tcttcaacag ctgctacgat tgaatcaccg aagtcaagac gccataattt
     7621 agagatatct tgtaaagcga tcatgtcgca atccatatat aagatacgtt caacttctgt
     7681 gccgcggaac aattccggaa tcgcaatacg ataataagct gtttctggaa tacgatcgct
     7741 gattaccaca tttgtaaaga attctttatt gattttcaag aattccacat ctgagttcat
     7801 tcgtgcgttt ttcactgaga agcgtaaaag ctgtttactt tcaaaatcga tatcatcgtc
     7861 aatcacatag aaccgaacaa atttagacgg gttgcaatta tctaatactg agacaaataa
     7921 tgcagctaaa tgtggtacga attttgtgtt acaacttgcg actactgcta tttctttttt
     7981 attcattgat ctcgcctact ttcgttagag tactgtgtgc taattttttt aagtataaat
     8041 tggtatacgg atgatctttc aacgtattcc aaggtttatc atgtcctgta aagtgaacga
     8101 tcgaaggttt ttcatttcct tctttatact gacgttcata tttttcattt ggtgctggat
     8161 gacgttcaaa aatcagcgaa gtctgcatgt tccattttgg atgaagttgt tcccaatctt
     8221 cataaagtac agcattcaaa gcatcttggt cgtgatagat gattcgatcg ccattttcac
     8281 tgaggtaatg gatagttttt tctgtaattt cttttttatt ccattgatca atgtcaatca
     8341 ccatcactcc tgaattgaag tacaagtcat cggaatcgat tcctaatctt tccaaagctt
     8401 tcacttggcc tggatcgata atagcgccga ttgtttttcc atttaacggc tcatcatata
     8461 gttttacgat atcgtctagt actaacacat ctgaatctaa gtaaaggact ttcttataat
     8521 cctctttagc taataagttt ggtaaagaaa ttcttaagta agcggtagtt gtgatatggt
     8581 cactgactag gaagtcttcg tagatatctt tttccactgt caggaattgg atacttgcat
     8641 tacttgaata tttatttacc gtttcttcca atccttcttt actatattct gatagaccat
     8701 cgtcaatcac ataaaatttt atgcgtcttg ttttattgca attttctaat gcagttgcga
     8761 tcatcacgct caagtaagga gcataatttt cgtctgaagc agttaccaca ggaactacgc
     8821 cgtatctctt ctccatcaaa atcactcctt tttctaatct ttttctaacc tgttctacat
     8881 ctagaatatt cctcatttgg ttttaaagca aaatatatgc tttgttttca ttgaatattt
     8941 taaaattttt ttatcacaaa acgcatcggt aagcgctaac tattcgtttt tcatggcttt
     9001 ttattcaaag gtattgacca aaaactacta tatatagcca cctttttctt aagaatgttt
     9061 tttgaaaaaa ttcataatga caaagcaagt tagtttcatt tttttaaatt ctcatttaat
     9121 cttaaggtaa caggaaaata gatatgtaaa aagaaagtag gaagataaaa tgaaactagc
     9181 agcgattgat ttagacggta ccttgcttga tcgaaatgga aagataccag aagaaaatat
     9241 ccaagcactt cggaaatttg atgaaaatgg cggaatcgta accgtcgcta ccggaaggaa
     9301 cagcatttct gctaaagata ttttttccga attaggaatc ggtggctatt tgatttcttc
     9361 caatggttca ctgattgctg aaatgaagga tggaaaaatg gcgaaagttc tcagacgttc
     9421 aaaaatagat agcgatacac tgagaaaagc cttcttctat gctcaagaag cgaaaatatc
     9481 catcattgct agccgagaaa cccaagatga tcagctgaca ttcgatgaat caaatctagt
     9541 caaagatgac ccttattatg aacatttcaa tttacagaac cattcttttg atcaaatttt
     9601 aaatatgttg gaagatcctt ctcttagcta tcttaaatta gcattaacag ataagagtga
     9661 agaaaaacta cagaagctac aaagagatct gcaagcagat ggtatccaat cagtattctc
     9721 tgatccgcat ttcttagaaa tcacgcctac aggtatcact aaagctcatt cacttgtttt
     9781 tctggcggat taccttggaa tcgaatcgag tgacatcatg gcttttggcg atcaagaaaa
     9841 cgacattgct atgttgaatt acagcgggtt gagtattgcg atggggaatg cacagcgaca
     9901 agtaaaagaa attgcaaacc aagtaacact gacgaatgaa gaagctggtg ttgcttatat
     9961 tttaaaaaac ttctattaaa aagaataaaa tgacgatatg aatgtaggag gaaagaaaat
    10021 gttagttatt attgatatgc aaaatcatat tcttgatcca aacagcgaat tttatattcc
    10081 agagtcagat caattggcag aacgtattgc tcaaagatta gctaaagcac gccaaaataa
    10141 tgaatatatt ctcttcacac gggatatacc agttgaactg aaggacaaag aagaagaacg
    10201 agaagatttg caattgatcc ctcttctttc tccacaagaa aatgaacgag tgattaaaaa
    10261 gtattacttt acgattccac ctgaaacttt agctgaaatc caggaaactt tatttgaaag
    10321 caaagaagaa caaaaagaga tcgaagtagt cgggattgaa acaaacttgt gtgtactctc
    10381 taatctgata ggcctgcaga gtgcttttcc tgaagcagac ttttttgttg atccaatgtt
    10441 agtctccagt agaaaacacg gagaatccgc tttagagctg ttgaaagatt tcaacgtctc
    10501 tatcgtcgaa tcataaaaat atctatcttc atttacgata tttaacacac aataaaaaaa
    10561 gcacgcagaa ctgttttagt cctacgtgct ttttctatca aagtagattg gcttttttat
    10621 gacacagagt gaaacggcag gctcacgacc tctttcttaa atggtgttca atcagctgac
    10681 cctcggtatt agcttaacaa acggcaaaat atgaggaact atttttccgt ttgttaagct
    10741 aatactcagg tcaaaacgct gatttcacaa cctcttcctt aaacggtatt caaaagcttg
    10801 catctgcagt aataagaaca gccaagtaaa aacataagaa gctgttttta cttggctgtt
    10861 cttattactt gatgcagggc agctttttca caacctctta tgtaccttgt aggactcgaa
    10921 cctacgaccg gacggttat
//
LOCUS       DAJSMI010000087        10565 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJSMI010000087 DAJSMI010000000
VERSION     DAJSMI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.087"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            266..535
                     /gene="rpsN"
                     /locus_tag="ON430_001790"
     CDS             266..535
                     /gene="rpsN"
                     /locus_tag="ON430_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR3219705.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            550..729
                     /gene="rpmF"
                     /locus_tag="ON430_001791"
     CDS             550..729
                     /gene="rpmF"
                     /locus_tag="ON430_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR3219706.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            761..1138
                     /locus_tag="ON430_001792"
     CDS             761..1138
                     /locus_tag="ON430_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219707.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1159..1308
                     /gene="rpmG"
                     /locus_tag="ON430_001793"
     CDS             1159..1308
                     /gene="rpmG"
                     /locus_tag="ON430_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3219708.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1332..2315
                     /locus_tag="ON430_001794"
     CDS             1332..2315
                     /locus_tag="ON430_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR3219709.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2345..2467
                     /locus_tag="ON430_001795"
     CDS             2345..2467
                     /locus_tag="ON430_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR3219710.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2485..4008
                     /locus_tag="ON430_001796"
     CDS             2485..4008
                     /locus_tag="ON430_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR3219711.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4032..4271
                     /locus_tag="ON430_001797"
     CDS             4032..4271
                     /locus_tag="ON430_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3219712.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4464..4925)
                     /locus_tag="ON430_001798"
     CDS             complement(4464..4925)
                     /locus_tag="ON430_001798"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3219713.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5120..5338)
                     /locus_tag="ON430_001799"
     CDS             complement(5120..5338)
                     /locus_tag="ON430_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3219714.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5456..7072
                     /locus_tag="ON430_001800"
     CDS             5456..7072
                     /locus_tag="ON430_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR3219715.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7313..8653)
                     /locus_tag="ON430_001801"
                     /pseudo
     CDS             complement(7313..8653)
                     /locus_tag="ON430_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8929..9084)
                     /locus_tag="ON430_001802"
     CDS             complement(8929..9084)
                     /locus_tag="ON430_001802"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCR3219716.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9380..9712)
                     /locus_tag="ON430_001803"
     CDS             complement(9380..9712)
                     /locus_tag="ON430_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3219717.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10226..10417)
                     /locus_tag="ON430_001804"
     CDS             complement(10226..10417)
                     /locus_tag="ON430_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3219718.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT         3505 a         1675 c         2066 g         3319 t
ORIGIN      
        1 gctacttaaa acggtatttg tgtttgattg gactaataaa agtgaattct tatttttcag
       61 agcctaaact gcatattctt ttaaatgatt tgattatctc tatgtaccga taaattcgtt
      121 ttcaaatagt tatctattca tatgagtttt atttcatgtt atttattgtt gacaagagca
      181 actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt acgatttgag gtgtctaaat
      241 caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg aaaattgcta aagcaaagaa
      301 acaaatggcg atgattgaaa agtatgcaga taaacgtcag gaattaaaag ccgcaggaga
      361 tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg aatcgtctga ggcttcgtga
      421 tcagacggat ggtcgtccaa gagggtatat gcgaaagttc ggtatgtccc gtattaagtt
      481 tagagaactt gcccatcaag ggttgattcc tggagtaaaa aaagcaagtt ggtaaaagga
      541 ggaaatatca tggcagtacc agcaagaaaa acatctaagg cgaagaaacg gttaagaaga
      601 actcatcaaa ctgtagtaaa accagaaatt tcatttgacg aagcgaatgg tgattataga
      661 cgtagccatc atgtatcttt aaaagggtac tataatggaa agaaagtaat taaaggagct
      721 tcaaaatagt ttaatatata acttttagga gggaatcaaa atggaaatta tttacccacc
      781 tttagttgaa gaaggattaa agtattatct tgaaacgacc cagcaatcgc tagataaatg
      841 cactttttat cgttcgatgg tagagagagg aattattact gaaacgggtt taccgaccca
      901 acaggcaatt gaaaatggat tagtcaaaga ttactatgag gaccaaggct tatcatttga
      961 tgatttttta agaatctatc caatttttga agagtatgac gaggagttat ttcaatgtat
     1021 cgatggttat tgggagatac ctgttgatat gaaagaaaat ttagtttcac aattggaatc
     1081 tggcgagtta acttttgaag atgcacaaca aattcaagca tatcttgaag atcgataagt
     1141 attgaaggag gaagatttat gcgtcaaaat attattttgg aatgtgtcga aactggagaa
     1201 cgtctgtatc tcaccagcaa aaataaacgg aataaccctg aacggttaga attaaaaaag
     1261 tattcgccaa aattgcgcag acgggcaatt tttaaagaag tgaaatagct aagtggtaaa
     1321 aaggtgacaa aatgggcata ccagttacaa tcgtttctgg gtttttaggg gctggaaaaa
     1381 caacgctaat caatcaagcc ttgcaagtct caccctaccc cagggaagaa attattataa
     1441 ttgaaaatga gtttggtgaa gttgggattg atcacaaatt attagttcat agcgaggaac
     1501 gggtgttaca actaaataat ggatgtatgt gttgcagtct tcgcagtgat ttattagcaa
     1561 tattgatttc tttactggaa acaatagaag aacatcaaca accaatttca caaatcatca
     1621 ttgaaactac aggtatagca gatccccaac caatcattca aacactttta acagcgcctc
     1681 aactaagagg aagagtatat atcgatagtt tattgactgt tattgatagt gacagttttg
     1741 aacttattga aagagaacca caagccctta agcaacttgt catggcagat cgcatttttg
     1801 tgtctagaaa acaaaacttg aatttatctg cactaaaaaa attacagaga gaaatcaaaa
     1861 gtattaaccc tttatccgat attcgaatgt tcacacatag tgaaacgatt caaaaaaagg
     1921 atttttataa tttgaaaaaa tttaatcatc ccttagtttt tgaaagtgat tcatcagaaa
     1981 atcactataa tggcaaatac tatcatcaac accatcaagt acatgagttt aaatcaattc
     2041 tcttaacttc agatcaagac ataaaaaaag attttttact ccaatggatt gactggctga
     2101 ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt gattgcttta caagagcagg
     2161 attttatagt tgccatgcaa gaggtaaacg aacaggttaa ttttcaatac accactcgac
     2221 aaaaagacca aaccactatt attctaatcg gaaaaatgtt ggatgaagaa aggatcaaac
     2281 agagttttga taaattactg gcggaaagca aataatgata agtagtgatt gggggtaatt
     2341 tctaatggag aaaacggata tatctagtgc atatcgacga cttaaaagcc cgaatattaa
     2401 aactagaaaa agggcattaa aaattatcaa agaacataaa cgaaataaaa tgaagaaatt
     2461 agcataatta aggaaggaaa aagaatgaag aaaatcagtg taggacttat tgggatagca
     2521 gctctaggat tattgggagc ttgctccagc actaatgatg caaaagtatc gaatgataaa
     2581 gatggaaaat tagaaattgt aacaacattc tatccaatgt atgatttcac caaaaacatc
     2641 gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg gatcagaacc tcatgactat
     2701 gagccctcag ctaaggatat ggcaaaagct catgatgctg atgtttttgt atatcataac
     2761 gaaaacatgg aatcatgggt accaaaagct aaagaaagtt ggaagaaagc tgggcctaat
     2821 gtagtagaag gaacaaaaga catgatttta ttgccaggta gtgaagaaga agatcatgat
     2881 cacggagaag aagatcacca tcatgaacta gatcctcata cgtgggtttc acccaaaatg
     2941 gcaattaagg aagtttcaaa tatcaaggat cagttagtta aattgtatcc gaaaaaagca
     3001 aaagtatttg agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt ggatgctgac
     3061 tataccacta gtcttaaaga agcgaaacaa aaaagttttg tgacgcaaca cgcagccttt
     3121 ggatatttag ctttggatta tggattgaaa caagttccta ttgctggact aagtcctgaa
     3181 gaagagccat cttctggtcg attagctgaa ttaaaagagt atgttaaaaa aaataagatc
     3241 aattatattt actttgaaaa gaatgctaat gataaaattg caaaaacttt ggccaacgaa
     3301 gctgggataa aattggaagt actgaatcct cttgaaagtc ttacaaaaga acaaatggat
     3361 aatggtgaag attatgtttc agtaatggaa gacaatctaa aagcgttaga aaaaacaact
     3421 atggttgctg gtgaagaggt agttccagaa aaagaagcga aggatgaaaa gacagttgct
     3481 aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta tacaggcgaa
     3541 tggcaatctg tttatccgtt gttaaaagat ggaattttgg atgaagtttt cgattacaaa
     3601 gcaaaaatca ataaagacat gacagctgcc gagtacaaag attattatac tactggctat
     3661 aagacagata ttgatactat caacattaaa gacaacacca ttgattttgt agtcaacggt
     3721 gaacatcatc aatacacata caaatatgtt ggatacaaaa ttttgaatta tgaaaaagga
     3781 aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg ttttaaatat
     3841 attcaattta gtgatcatgg aattgcacca agtaaggcag agcatttcca tattttcttt
     3901 ggtggagaaa gtcaagaaaa actctataat gaattgcata attggcctac tttctatcca
     3961 gcttcattgt ctaaacatga aattgctcaa gaaatgatgg ctcattaaaa ttcaattttt
     4021 ggaggaaata catggtaaga aaagtattat tgtctatgga ttccaagaat attgatgctt
     4081 atcggcgtta tcgatttgca aagcaaatga gattaaatgg gagtacatta gctgatattg
     4141 ctgcaacttt acatgttagt attgacacag ttcatagagt attaaaacgg gatcctgatt
     4201 gttgttttat caaggtcaat actggaagaa gtagacgttt agcaaaacgg cataatagct
     4261 tggccaatta attgataata ccagagtaga tcatttttcc cttatttgga tttgatctgc
     4321 tttttattat gtcttgaatt gtcaaatcaa gcgcaacact ttatcaaaga agacttcata
     4381 aggtgttctc caatctaaac actttctagg tctgttattc agtttttcaa tcatctgatt
     4441 tatcgttgag ttatctaccg tagctatctc tttcgatttt ggcaagtatt ctcgaatgag
     4501 tctattcgta ttttaatttg ttcattgttg ccaagggcat gaggatctgg aaaataaaag
     4561 ggcatattat tcaatgcttc tgtaactgcg aaatgattgg agaattcttt tcctctatct
     4621 ggagtgatcg ttttgaccgc ctcatttgga atactgctta aaagttcaat cattttctcg
     4681 ctcacataag cggctttctt tctggggacc ttctttcctg ctaaaaggaa gcatgatttt
     4741 ctgtctgtca aagtcactaa acaagtacca ccagttttgc cagctacaat atcggctttc
     4801 caatgaccaa aatcacttct atcatctgcg gattttggac ggccatggat cgaatgagta
     4861 aggcaaatct ttccacgaga ctcgacatga ttttttgtat gtcttgtttt tcctcgatgg
     4921 tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg gttctgtatc gaaaagatca
     4981 ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa agtccatatg ttcatgcttc
     5041 aaacgattcg agatttcctc tggcgaccac tgaaaatcga caaataaatg acgaacaatt
     5101 gaccaaactc acggatcatt tagaatgtga taacgtccgc atttttttct tcgataagca
     5161 taattttttt ccgctttgga aggtgaatag tttttttctt gtttcttctc agttcatgta
     5221 aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat actaatagaa aatccttctt
     5281 cgtgaagcag aaagattctt tctcgttcga gtatagtaag atgacgatac tggctcataa
     5341 ttattttcct tctaggagtg ttctcgacaa acttatacta gttggatatc gattatgact
     5401 catttttatg tgttgcactt aaagtgtaaa ttcaaggtat agaaagaggt tccctatgaa
     5461 aaaaatacct attacaatct taactggcta tcttggttca ggaaaaacga tacttctaaa
     5521 tcatattctg tacggtgatc acggaaagga aattgctgtc attgtaaatg acatagggga
     5581 agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt aaaagaactg gagaaaaact
     5641 tgtttcgatg ccgaatggat gcatttgttg tacattacgc gaagatttga ttgttgaact
     5701 agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt attgaagctt caggaatcag
     5761 caaaccaatt ccaattattc aagcaattat attagctaaa aaccctttag acattgattt
     5821 gacccaacga ttagagattg gtgccattat tatagtagtg gatgtaaaac gtttttggga
     5881 taatttcgaa acgggtgaaa agattcagga aagaaaagct gaaggtaatg tagttgaaga
     5941 agaacaggat attcgtggct tgttaattga tcaattcgag tgttgtaaca tattactttt
     6001 aaataatagt gacttagtgt caaccaagac gatttacaaa attaacggtc taattgctaa
     6061 gttacagtcc aatgccaaaa taattaggac aattaaaggt gaagtagact tatcgaaaat
     6121 tctaaataca aatcttttta atatagatga gacttcctat tcagtgggct ggttcaaaga
     6181 gttagagctt ggctatcaaa atcatattcc tgaaacagaa gaatatggta tctcaagttt
     6241 tatctatcga aatcggttac tccttgacgc acaaaaattt gcccatctta cggataaaaa
     6301 ttttcctggc aatattacaa gagcaaaagg gtttctttgg tttgcccaac atagtgattt
     6361 ttcgacgatc tttgaaacat cgggtagcta taaaagtatt gaatcattaa attattgggt
     6421 agctagttta cctaaaattg agtgtcaaat gattatacaa aaaaatcccg aattgaagga
     6481 agcctggcat ccgttgtttg gtgaacgcat taataaacta gtttttattg gtatgaagat
     6541 ggatcaacaa tgtattaagg aacaacttga gggttgttta actgattctc ttaatgcaga
     6601 taatttccga aaaatttcac catttccatg gaaaaagtct aatatgttta gccagatatc
     6661 gttaatgaaa tttttaagaa aagctggtgt caagataggc gatgtcataa ttattggcat
     6721 tttgattatt acctctttta ttcccttatt tgtattctct atagaaaagg agaatgaaac
     6781 aactgaagat gctatcaaat atgccatcgt tcgaattgat ggcaaggaaa tggatcgctt
     6841 cgatttggat aagatcaatc ataaactcgt gaattatcat cctgcgaagg gacaatataa
     6901 tattgttgag attaaagatg gaagaattcg agttaaagaa gataatagtc ctgatcaaat
     6961 cgcggttaga actggatgga tctcaaaacc cggtcaaacg agtatttgtt taccccataa
     7021 gttagttatt agtattgaga aaaaagaaag cacagattac tacatttact aatctggaaa
     7081 tagatgagga ggtttttaat aagaaaaaga tattattatt aataatagca ggggctttat
     7141 taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc acaagtatct
     7201 ttaacgggac ctgcaatggt ttagttaaca gtcggttaga ttacttattt tggctcttcg
     7261 tcaatgagtg ttggtgaaga aattcaatag aataatcgag atactcacac ggctagacag
     7321 ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag ttgggtagta ttcttagttt
     7381 tcttaaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga aagtttctat
     7441 agccataggc aattcgtttt agtactttga tatggttatt caaacattct atagggccgt
     7501 tggaataggg aacgcgcaac gctcgtttga tttctgtttg gtatttcttg aaggtcgtga
     7561 acactggttg gtagtgctct ggcaactcct tgtagtcttc ttttaggagc gcgacgaatt
     7621 caggaacatc ttgtcttctg ataacggaaa gaaaatcctg gtagacttcg tagcctcgct
     7681 ttaaggaatc atcataagat aatagccgat cgactatctc tgcttctgtc agatgatctc
     7741 gaaatgacgg acgccagtag cgatgctgac ctttcaattc ccacgcattc ttttgaagta
     7801 gtttccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag gtcttcatta
     7861 cttgaattcg gtggttggta aaagcccgac caatgtgttg gctaatatga aagcgatcgg
     7921 cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt gtatagaaat
     7981 ctgaaaccac tagacgcact tgttcacgga cttttatagg gtagcgcaag aaatagtcgc
     8041 gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc ttgccgtcca
     8101 tcatgacaaa gctcatggct cccgagacat ttttcactga tttgaattca tcgaaacaca
     8161 ccacttctgg taacagctgg ctcaaagaaa tggttttagg ccgaaggctg cgaagtattc
     8221 gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat caaagacata gaaagaggtt
     8281 cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc aatggagcag cgacgatcac
     8341 taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag cgctgtttgt
     8401 taatccgcag aatggtcttg tagttactga catcattcaa taagatggat acttttttcc
     8461 agccctattt aatgatcgtt tgttcatttt gatagtggcg atgaaagcat tcctccggtt
     8521 gataggtcaa tgtgccatca aaagcaaaac atgtttcgtc attgatgcgc tctttgcgaa
     8581 aggcatcctc atgaaaggta agatttaggt ctagaatatc tagtaactct ttggtaaact
     8641 ctgaatagga catcttgaaa cactactttg aatggttttt gtcgacttta ttctaaagga
     8701 ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa cagaaggcgt
     8761 caccaacact agatattata gatcccttat tttcttcgct tcaaattgtc aaggagtcat
     8821 cataaatttt gtatggattc ttttgtttgg gtagtagtct tctaaataat cttggattaa
     8881 caccttcatc tctattttac ttttatattt gttggagtcg attaattctc atttgattga
     8941 tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc ccagtttggc tcactgaatg
     9001 tgtgatgtgg tggtcgttca acagctcttg ataaataaaa gaccgatgct ggctacctct
     9061 atcggaatga attaccgtag gcaattctcc ttaataaatt tgtaatggac ggacgacaat
     9121 ctcttttttt atgtgtgaat ctaccatata gtaacgtaga atacgagttg ctaagtcaat
     9181 ataggtggac aaatagaggc gaccgtcatt acatggaatg taagtaatat ccatcaccca
     9241 aacagcatca atttggttct gttcaaatta tcgattcaac atttttttac catttctttg
     9301 attgtttttt ctctaccatg agaagatttg cgtcgatgaa atcctcttgt gcataaattc
     9361 atttcacgca taagaacagc tacaacacgt ttattgattt cgattccttc atcgtaaagt
     9421 ttttgagtga tccctggaga tccgtatcga tctttgtgtt cgtagaacac tcgtcttcct
     9481 cttttagtcg tttattttcc tgttcaagaa tgtagaaatc tgctttatca ggtaatcttt
     9541 ttccttgtcc agcaaaagca tttttcccgt atcgacgata ttctgaaacc catcgggaaa
     9601 ttgttggacg agagatattg tattcttcag caattttctt ggtagttact tttttctcaa
     9661 ggatatcctg tgaaatcttt agtttaaatt tacgatcata tgtttttctc aatagagttc
     9721 attccttccg gtaacgtaac tgatagtcaa gaaaagtgta tcatagccca acttagtatc
     9781 agggtttgtc aactaaactg tggaagttga tagataagag tttttaacaa ccaaaattct
     9841 cttgactatt tttgaaatca gacaaatttt gattggacac atagttgaat aaatctaccg
     9901 tcgttacgga aggtaaatcg catgcttttc aattctagag gagaagtcct cgttgaagta
     9961 ccatagccca gttctggatt cgacctcctg cccacttcat acgtaaataa agtaatttta
    10021 ggagtgagac tgtgacaaaa gttattttta agctaaaaac gaattaaaaa acgttgataa
    10081 ttaaaatcat tcgttttaga ttctatgaat cattatagaa cgctgtaaaa tcgatacttt
    10141 ataattataa agaagatagt ttcaaattta ttgttcgaaa tttgaaacta tctttttact
    10201 tatgtcacag actcccttag ttgagttatt ctttaatttc gaatttcgag atagctagat
    10261 atattgcaag catacattct tgataacaat cttgatcata ttttccagtt ctccaggatt
    10321 gcttatgaat caaagagcta aatcttttga gaattgcctc aacagcttct tcatttccag
    10381 cctttgctaa aatgaaatct tcttttaaag aattcataaa tctaccttct tcccatggac
    10441 ttataatcta tttctttggg aaggaggaga acgagcaaag aagcatacaa aattagttat
    10501 tatgcagata ttaaaccagc attttttgaa tttttttgta aagtcgatgt tttaaatttg
    10561 tcgca
//
LOCUS       DAJSMI010000088        10422 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJSMI010000088 DAJSMI010000000
VERSION     DAJSMI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.088"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            5..229
                     /locus_tag="ON430_001805"
     CDS             5..229
                     /locus_tag="ON430_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HCR3219719.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(434..754)
                     /locus_tag="ON430_001806"
     CDS             complement(434..754)
                     /locus_tag="ON430_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR3219720.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(902..3136)
                     /locus_tag="ON430_001807"
     CDS             complement(902..3136)
                     /locus_tag="ON430_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3219721.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3615..3881
                     /locus_tag="ON430_001808"
     CDS             3615..3881
                     /locus_tag="ON430_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCR3219722.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3881..5608
                     /gene="ptsP"
                     /locus_tag="ON430_001809"
     CDS             3881..5608
                     /gene="ptsP"
                     /locus_tag="ON430_001809"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR3219723.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5772..6818
                     /locus_tag="ON430_001810"
     CDS             5772..6818
                     /locus_tag="ON430_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3219724.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6835..8058
                     /locus_tag="ON430_001811"
     CDS             6835..8058
                     /locus_tag="ON430_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3219725.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            8147..9034
                     /locus_tag="ON430_001812"
     CDS             8147..9034
                     /locus_tag="ON430_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR3219726.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            9120..9350
                     /locus_tag="ON430_001813"
     CDS             9120..9350
                     /locus_tag="ON430_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCR3219727.1"
                     /translation="MAHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9407..10372)
                     /locus_tag="ON430_001814"
     CDS             complement(9407..10372)
                     /locus_tag="ON430_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3219728.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3367 a         1830 c         2111 g         3114 t
ORIGIN      
        1 acacatggaa acagaatggc aaagacgtga aagaaaaaaa cagaaaaaag aagagatgtt
       61 acgattatgt cgattattag atgtaaaaga ctgggacaaa aacgagcaaa ttgcacaaaa
      121 agtacgatca atcatccata aagaggaaaa agttaaaaat aaagacacgc gtagaactga
      181 aaatgaaaag agaaaagtac aaaagaaaag agaaaaatca aaaagctaga aagaaaaaga
      241 ggctgagaca gaagcgttta actccaagaa ataagctgga attcacaaaa atttgaaaag
      301 caattttcgt gaatttcagc ttatttccga aggagttgct tctgctttcg ccgtttatac
      361 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt taattattat
      421 gttcaaaatt tgtttacacg gctaatgtag cgcctaattc atgttttgga atcgagattt
      481 caacaaggcc tgccaattcg attactttga tttcatcctc gtggtaaacg gcgtctttga
      541 ttttatctaa gaaataattt ttagcatctt cgatttcttt cggctggatt ttacgtttgt
      601 cattgatcag cagaatttca ttatgttcag gggcttcagt gtagacaata gtggtatttc
      661 cggctgtgta gaccttgact agatgggcat cagtgttttc aagctggttt aatacaagac
      721 gggaatggct gtttgttaca tttaccaatt tcatggcgct cacctctttt atgtattttt
      781 aattgcttgt ttctcttatt ataatttctt ttttataaaa tgaacagtat tttgactttt
      841 ctgaaaaaag aatgagacgg catttctcaa agaaataccg tctcacgcta taaaactgga
      901 tttattcagc cgtttcttcg gctgattctt tagccaaacg ttctggttta gaagtaacga
      961 tgatttttcc atctttatcc agttttgctt tcaactcatg gatagaaggg tgatcaagat
     1021 aaaattcagc gataccatct tcgatttgtt cttggatcgt gcgtcgaaga ggacgagcac
     1081 ccatagatgg atcatagcct aagtcaacta atttttcttt gacattcgta ggaacttcga
     1141 tgtgaagttg ctgtgctgcc agcatttggt tgacatcgtc tagcattagt gtgacgattg
     1201 tcatcaagtt ttcttttgat aatgcactga attcgatgat cccgtcaaaa cggttcagaa
     1261 attctggtgt aaagtagttg tttaattggt tcaatacaga acgagtaaca ccttcgcgtg
     1321 ccgcaccaaa accaacgttt gcttctactt taccagttcc ggcattactt gtcataatga
     1381 tgatcgtatc tttaaagctg accgttcttc cttgtgcatc tgtcaatcga ccatcatcta
     1441 agatttgcaa gaacatatgc aaaacatctg gatgagcttt ttctacttca tctaacaaga
     1501 caagactgta aggatttctc cgtacttttt ctgttaattg accagcttct tcgtatccga
     1561 cataacctgg aggagaacca atcaatttag agacactatg tttttccata tattcagaca
     1621 tatcgaaacg aatcattgaa tcttgtgaac cgaatagttc gtaggcaagt tgtttcgcaa
     1681 gctctgtttt acctacacca gttggtccaa caaataagaa ggagccgatt ggacgattct
     1741 gtttgtttaa tccaacacgg tttcggcgaa tcgctttcgc tactcgatct acagcgttat
     1801 cttgtccgat gacatgagct ttcaagtcat ctgctaagtt tttcagttgt gtctgttctt
     1861 tttcttttaa ttcacccact ggaatacctg tccgttgttc aacgattttt tccatatctt
     1921 tttcggtaat cacaggtgtt tcctcttcag tgagctgacg ttctttcatt ttttgaagtt
     1981 tattgatctg atcgcgataa taagctgctt tttcgaaatc ttcttctcga gaagctaaaa
     2041 ctttttgttc ttctgcttct tgcagttttt tctcgattgt tttaggatca acgatttgga
     2101 tcgtcaagtt tttcttagaa cctgtttcat ctagcaaatc gatggcttta tccggcaaga
     2161 agcgatcttg gacgtaacga ttggataaag ttgcggctgc ttcgatggcc tcatcagtat
     2221 attttacatg atgatagtct tcataacgtt tttgcaaacc tttcaaaata ctgatcgttt
     2281 cttcgacagt tggttcatct acccgtacag gttgcatacg acgttctaaa gcagcgtctt
     2341 tttcgatgat gcggtactca tttaaggtag tagcaccaac catttgcaat tctcctcgag
     2401 ctaaagctgg ttttaaaata ttaccagcat ccatatttcc atcaccagca gaaccagcac
     2461 cgacgatttc gtgaacttca tctatgaata ggatcacatt ttctgcttga cggatctctt
     2521 caatcaattt ttgcatacgt tcttcaaatt gtcctcgtat accagtacct tggactaaag
     2581 atacgacatc taaacgaatg acttctttgt ccattaattt ttgcggtaca tcaccatcta
     2641 cgattttttg tgctaaacct tcaacaactg ctgttttacc aacacctggt tctccaatca
     2701 agacaggatt gttcttggtt cggcgattta aaatttcaat gactcgtttg atttcgtcat
     2761 cgcgtccgat cacaggatcg atatcacctt gtcgtgcttg ttctgtgata ttgattccgt
     2821 attctcccaa taaaccttct gtattgcctt gaggtggctg accaccattg aagttgttgt
     2881 ttccgccaaa ttgtgttggt ggtgtttgct cataatttcc ttgctgttgc atttggcgag
     2941 acatggaacg gaaaagatcg tccaaactgc caaatccaaa tggatcattt tgggccatat
     3001 tagtcagacc tccattttgt tgatttttga ttttttgata acagctctga cagtaatcga
     3061 gttgtgtccg ctgaccattg acagtggcat acaagtgaat cgttgcctca ttcttgccgc
     3121 agttttgaca aagcatgaat cattcacgtc ctttcacaaa aatgggtaca tccatatttt
     3181 acatctcgtt tttcatgagg taaaagaata acccctagtt ttttaagaga tttatgaggt
     3241 agaaaaggtg aaatctctat tgaccattcc tgacctttga ctcttattat actaaaaata
     3301 aaaagggaag caagtaattc gtttcatatg aaaacaaaga gggtcaaagg aaagaatgat
     3361 aaacgtcagg agaagagaaa gcgacaagaa aaaggaaata aacacgtttg ttataaaatg
     3421 tgaagaaaag gatgaaaaag aatccggaat aatttgaata cttttatctg aaattgaatg
     3481 aaaactgttg tatctaacga aaaaaaatgt taatctttac agatgaaagg gtggcatttg
     3541 ttaggattct ctgaattact aaaaaagaaa accctcacat aaaaacaaca ctcacttaaa
     3601 ggagaccgat taatatggaa aagaaagaat ttcacgtagt agcagaaact gggatccacg
     3661 ctcgtccagc tacactatta gtacaaacag caagcaaatt taactctgat gtaaacttag
     3721 agtataaagg taaatcagta aacttgaaat caatcatggg cgttatgtct ttaggtgtag
     3781 gtcaaggttc tgatgttact attactgcag aaggtgctga cgaagctgat gcaatggcag
     3841 caatcgttga aacaatgaag aaagaaggct tatctgaata atggttgaaa tgctaaaagg
     3901 gattgccgct agtgacggtg tagccgttgc aaaagcttac ctgctagttc aaccggattt
     3961 aacattcagt aaggcaacag tagaagatac tgcggctgaa gaagctcgtt tggatgctgc
     4021 attagctaaa tctactgaag aattgcagca aattcgtgaa aaagcagcgc aaagcttagg
     4081 tgaagcagaa gcgcaagtat ttgatgccca tttgatggtt ctttcagatc cagaaatggt
     4141 aggtcaaatc aagcaaaata ttaaagacaa cagcgtaaat gctgaatcag cgctaaaaga
     4201 agtaacggat atgtatatcg gtatgttcga agcaatggaa gataatgctt atatgcaaga
     4261 gcgtgctgca gatattcgtg acgttgctaa acgtatttta gcgcacttat taggcgttac
     4321 tttaccaaat ccatcaatga tcaacgaaga agtggtcgtt gttgcacatg acttgactcc
     4381 aagtgataca gcacaattag atcgcaattt tgtaaaagcg tttgtaacag acattggtgg
     4441 acgtacttca cattctgcta tcatggcacg ttctttagaa attccagcaa tcgttgggac
     4501 aaaagaaatt actgctaaag tcaaagaagg cgatattttg gctgtcaacg ggatcgaagg
     4561 cgatgtaatc atcgatccta cagatgaaca aaaagctgaa tttgaaaaag caggtgcaga
     4621 ttacgcagca caaaaagcag aatgggaaaa attaaaaaat gcagaaactg tcacagctga
     4681 cggcaagcat tttgaattag ctgcaaacat cggaacacca aaagatctag ttggtgtcca
     4741 taataatggt ggagaagcag ttggtctgta ccgtactgaa ttcctgtata tggattctcc
     4801 agacttccca acagaagatg accaatatga agcttacaaa gctgtattag aaggtatgga
     4861 aggaaaacct gtagttgttc gtacaatgga catcggtgga gataaagaac taccatatct
     4921 gcaattacct catgaaatga atccattctt aggatatcgt gcattgcgta tcagtttatc
     4981 tgaacaaggc gacgaaatgt tccgtacaca aatgcgtgca ttgcttcgtg catctgttca
     5041 tggtaattta cgtatcatgt tccctatggt agctacactc aaagaattcc gcgcagcaaa
     5101 agcgatcttt gaagaagaaa aacaaaaatt gataagtgaa ggcaaggaag tatctgatac
     5161 gatccaagtc ggcatcatga tcgagattcc agcagcagca gtcttagcag acaagtttgc
     5221 aaaagaagta gatttcttct ctgtaggtac gaacgatttg attcaataca caatggcagc
     5281 agaccgtatg aacgaacgcg tttcttactt gtaccaacca tataacccat caattttacg
     5341 tttgatcaaa aatgtaattg atgcagcaca cgctgaagga aaatgggcag gtatgtgtgg
     5401 agaaatggct ggagatcaaa cagctgttcc attgctagta ggtatgggtc tagatgagtt
     5461 ctcaatgagt gcgacatcta ttcttaaaac gcgtagttta atgaaacgct tggacacagc
     5521 gaaaatggct gaacttgctg atcgtgcatt gaaagaatgc gatacgatgg aagaagtcgt
     5581 tgaacttgtt catgaatatg taaaataaac aacgtatcaa aagctggtgt ggcttgtaaa
     5641 agagctacgc cagttttttt gttctttttt aagaaaacgt ttttgtcagt aagaatagac
     5701 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
     5761 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
     5821 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
     5881 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
     5941 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
     6001 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
     6061 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
     6121 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
     6181 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
     6241 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
     6301 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
     6361 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
     6421 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
     6481 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
     6541 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
     6601 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
     6661 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     6721 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     6781 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     6841 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     6901 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     6961 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     7021 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     7081 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     7141 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     7201 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     7261 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     7321 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     7381 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     7441 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     7501 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     7561 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     7621 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     7681 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     7741 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     7801 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     7861 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     7921 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     7981 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     8041 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     8101 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     8161 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     8221 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     8281 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     8341 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     8401 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     8461 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     8521 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     8581 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     8641 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     8701 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaggtga ttgagacgct
     8761 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     8821 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     8881 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     8941 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     9001 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     9061 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     9121 tggcacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     9181 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     9241 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     9301 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     9361 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     9421 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     9481 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     9541 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     9601 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     9661 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     9721 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     9781 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     9841 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     9901 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     9961 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
    10021 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
    10081 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
    10141 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
    10201 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
    10261 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
    10321 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
    10381 cttttacgct ttgaataaaa agactataac aaattttgaa at
//
LOCUS       DAJSMI010000089        10210 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJSMI010000089 DAJSMI010000000
VERSION     DAJSMI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.089"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..214
                     /locus_tag="ON430_001815"
     CDS             <1..214
                     /locus_tag="ON430_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016614869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR3219729.1"
                     /translation="EEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQ
                     ALKDIKEGNVHGGFSSVEDLMEDLNA"
     gene            207..482
                     /locus_tag="ON430_001816"
     CDS             207..482
                     /locus_tag="ON430_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HCR3219730.1"
                     /translation="MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKY
                     KDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTLARIGSHSELFR"
     gene            1285..1470
                     /locus_tag="ON430_001817"
     CDS             1285..1470
                     /locus_tag="ON430_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219731.1"
                     /translation="MNELQLKLDLEKAQLEYQKLSQAINENDTVTLLLNYGCLKNAND
                     RLNQLSFLLNHIEWKDV"
     gene            1492..1638
                     /locus_tag="ON430_001818"
     CDS             1492..1638
                     /locus_tag="ON430_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfM protein"
                     /protein_id="HCR3219732.1"
                     /translation="MTIVETLEVAVYVYAESEEQAHELVSNMYYDQEIILTEENHETT
                     EFII"
     gene            1869..2648
                     /locus_tag="ON430_001819"
     CDS             1869..2648
                     /locus_tag="ON430_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219733.1"
                     /translation="MDFQNIQTVKELTHALKQYNQNLATTIFDDTQGYISVAFKKDKI
                     EKNGNDFQFLCFYPNYEVALYPVVTLLSYLEKLPEQATVAIKDPDGNYSSLYLTIDET
                     IDKGTKYKWLVISDRLSYERFYLTSQLTIQEQALEKMLKELETEPNNRLEAIHDWLCQ
                     QTDKDLLVGILKENRTLKGAEAYCTQKAYEIRNGNGAMASHITVFNWVREYYTLDELP
                     TDKPKIQATIKSDYINQKIQRPTPVETKNNNEIDLFEGEDF"
     gene            2648..3907
                     /locus_tag="ON430_001820"
     CDS             2648..3907
                     /locus_tag="ON430_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HCR3219734.1"
                     /translation="MNTTDYVENKLQPPRAFFEWCYSSFRTYVWKNKNETIVSSTRKH
                     DWIIEKKLRKNSRLTFYDSSSCFQIILSTSKRIEVQTYKVISEYENGVQCFREQLECI
                     EIFSNNQHIKIGKICLPVYYGYNMGIALYPNEWKKRLERVSELKYLNLERLNVDNLAT
                     TYKYRTLIEFAQKINAHKLAYDVMSGAVDMRILTKNCLRKYKTFLKNTDNSLKEIQLK
                     QTFESLNIPMVKGIEKYVLKSDISDFPNEIGAVKFQNWLVKQGKSFKYYQDYLNMLTL
                     LKIEINKRNQLPPDLEVAHDCAVDRINQLNYEKRDKEINERLKQLRKYERDIDGYTFV
                     LPKRANDIKKEGKALNHCVASYISRHAKGETTIIFVREKKNPQKSYFTLEYNYNRVVQ
                     LQGKKNRQKVPDELKQAVDKWVKVIKD"
     gene            4557..5285
                     /locus_tag="ON430_001821"
     CDS             4557..5285
                     /locus_tag="ON430_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219735.1"
                     /translation="MTRLEQAQGKLQRLKRESEEMHHLIRAEHDRIPFGQPNIIGRGD
                     IYKKVNGYHDRAIKLLKEQEKQEKRVEMLEKVEDFKEKNELIKDVHVVGKSSYATVGA
                     KTSVNNIDYFKNELKELEKANEKAKAYNKTKPAIKARTFGAVITKLKNKIATLEQMKE
                     ADENKVVSERTKELIESGAVTQWKKKPIFYFVKGLRKVALEIDENGEFFISNYYPACT
                     DADKEFISKLLDPAAESRKKETFC"
     gene            5496..5906
                     /locus_tag="ON430_001822"
     CDS             5496..5906
                     /locus_tag="ON430_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002394836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219736.1"
                     /translation="MANETLKKEAWYRMMLTDLSSSVIDEFMETGKCHYTSNYFQGEN
                     ILVTEEIEAIIKTVEKKYGFLVYYVTENKTADGQRFLSLFYVGRDTSDWLYCHRDLES
                     YRQYVYVVNTTNPAFSEFGMIQFDPILGSLLRTA"
     gene            5992..6330
                     /locus_tag="ON430_001823"
     CDS             5992..6330
                     /locus_tag="ON430_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219737.1"
                     /translation="MSMEIRELIIIEDPCFYQYTTIKELESFNTSELTIALSVLRKEQ
                     RRLKEKVYHSGSGDQVDKNNVKLINKYRANLEKLLFIKQGYIPDAITQNMIDTLIKNN
                     KKSIQKIQKG"
     gene            6379..6552
                     /locus_tag="ON430_001824"
     CDS             6379..6552
                     /locus_tag="ON430_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219738.1"
                     /translation="MAEKIDLNKLYHESKKERTEKQTKMERALEGVVCGFPRNTDDDP
                     ILKNSKTQENTNG"
     gene            6545..7204
                     /locus_tag="ON430_001825"
     CDS             6545..7204
                     /locus_tag="ON430_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HCR3219739.1"
                     /translation="MDEKRKDKIQRLLALANDANDEESMTALAKAQELMLAYGIDEEE
                     VFHYQQHQRQEKVIDKVIYKGRPHKWVYRLAGIIAKNFRVKNYYQSSSDIELHYVGLT
                     TDVEIAEITFLYAKGSVAFCSKEFMKQPEIKRKYKRKWQLKQDYIEGYLTALSSVFEA
                     QVLTNGYELALQLPVAVHEEVSKLGLVKGKDTSHHVENFDAFHSGYQEGLQFKSKELI
                     N"
     gene            7294..7491
                     /locus_tag="ON430_001826"
     CDS             7294..7491
                     /locus_tag="ON430_001826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219740.1"
                     /translation="MKFLIRTIGGAEFVDVLNLMHAQGVVIGLKKTYAVNVYRKRTKK
                     ADKKLFAFFTYIFYTIKKRGF"
     gene            7595..9055
                     /locus_tag="ON430_001827"
     CDS             7595..9055
                     /locus_tag="ON430_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase domain-containing protein"
                     /protein_id="HCR3219741.1"
                     /translation="MNKFQSWLMANKNLLITSVVMCQVLVLSIGIINFPYIDDTGRQI
                     DGNTDFARSYSRWGSEIGSWLVQGSRHLTDMGLWTHILSGFIVSVTGIIIVYSFHKKL
                     SLLALTAAMLVGFNPWFLQNLSFRFDSPYMSLSLLFSVLPFLFWNKNKVLFLVVSILS
                     IFLMCNTYQASSGIYVVMVLALSLKQLLDNQSFIAVLKKAIVAMISYISAMFLYLIET
                     KFNPEIATRGGMTTIASVKDIPKTILVNSQMYLSKITEQSTKLWILLFFILVIFFVLS
                     TVLNAKVSPVKSFLYAVLFLFLGSILSYGVFLIFPEKLLLISPRYGYGFSFFVVSIIM
                     LTLSNTSKNVLGYVSKTAVCLFCVYLLSFTFVYASALSHQKESFERQSMILADDLKDL
                     VNRDTVTVHSTSLFKDSPVFANSSKNYPILKELVPPNEALYWPNQFLFRTYTGLNVNM
                     EIFDINALNKEESELMKSNYYHDIYVKDSEVFVHVK"
     gene            9435..9809
                     /locus_tag="ON430_001828"
     CDS             9435..9809
                     /locus_tag="ON430_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR3219742.1"
                     /translation="MENIFDLNKGLSRRKYWYYQCILFVFGLGIETFSMFVYKKSLIE
                     NNYLKYFVINGPFDFYNFLRIVFFIMIIIGFLFQARRFVDIGWSKFVALLSLLPLVGI
                     ITTGICLFKKGKDNKTDSSQVS"
BASE COUNT         3723 a         1585 c         1977 g         2925 t
ORIGIN      
        1 agaagaagca gaacaattat ttgatgattt agggttaaat atgacaagtg caattacgat
       61 tttcttaaaa cagtctatta atgagcaagc aattcctttt atgattaata agggaaacaa
      121 agagactcta caagcattaa aagacattaa agaaggaaat gttcatggtg gattttcttc
      181 cgtggaggat ttaatggagg atttaaatgc ttgaaatatt ttacacgaac caattcaaaa
      241 aagactttaa gaaagcaaag aaacaaggaa agaatttaga gaaattaaaa gaagtattag
      301 tacttttaca agaacagcaa acattgccgc caaagtataa agaccatgca ctaacaggaa
      361 attatatagg tacacgagag tgccatattg aaccagattg gttactgatt tataaaattg
      421 acggtgataa attgatccta actttagccc gaataggctc acatagtgaa ttatttcgat
      481 gaatcggaaa gatattacaa atcaataaaa aacactacta aacgaccaga cgaatagtag
      541 tgttttttga ttatgcgatt gtactaagca attttaacac aataaaaaat aatcatgcaa
      601 gactagggcg caacaatcta ttatagacgt gcgccttttt tagttcggga aaagggaatg
      661 aatccgttgt tccgcggcgg tgcggtagcc cttgctccaa aaccaacgga ttaactgctg
      721 cactcaacac gagtcaatta aacgttctct acccattcgt tccgattggg gaacgtttcc
      781 ttttcctact cgttcctcgc ttaacggaaa aggccattga cacgagttgt tccttgtggg
      841 atcacaagga agcgttccgc agtgattaac atttctagta ggtatttcat acctactaga
      901 aaacaaagtc aagggcgttc cggtcaaaaa tcccttttct gatgtcagaa cagtgtcacg
      961 cgaccttttt cgttttcagg ccgttcattt tgcagtgtgt aacgcgctgc tcactgtttg
     1021 ctacagtacc acagccagtc gaaaaaacaa gggtatataa acgagcaagc tcgcccttgt
     1081 tttttcttta tgtctatggt ccttccacgg cacagcgagc gacacgaaca cactggcgaa
     1141 aatgaacaac ctaaacaaaa acgaaaaagg gggtagacta caccgtttga cgaaaaggaa
     1201 tttttgaccg tccagcttca cttcctcaaa cgcctaacaa aaatggttgt tcaaaaccca
     1261 aaaacctgaa aggaggtggt taaaatgaat gagctacaat taaaacttga cctagaaaag
     1321 gcacaactgg aataccaaaa attaagccaa gcaatcaatg aaaatgatac tgtcacgctg
     1381 ttattaaatt atggttgttt aaaaaatgcc aatgaccgtt taaaccaact atcatttttg
     1441 ttgaaccaca ttgaatggaa ggacgtgtaa gaatggctga aaaaatactg tgtgacaatt
     1501 gttgaaacct tagaggtagc tgtatatgtt tatgcagaaa gcgaagaaca agcacatgaa
     1561 ttagtttcta acatgtatta cgaccaagaa ataatcttaa cagaagagaa tcacgaaaca
     1621 acagaattta tcatttaaaa caacgaaaga ggtgaaaaag atattttaga ggtattgaat
     1681 tatatccacg aaaaaagcac aaaaaaagaa accttttgcc actaacaaaa gatttctaaa
     1741 gaattggtag ttatacctga ataaatttgt aattcaagta taacgtatct catgaccttt
     1801 gacaagtgaa tttcccccaa cctcttattc ataagaggtt ggactaacaa aagaaacgag
     1861 ggaacgttat ggattttcaa aatatacaaa ctgtaaaaga attaacacac gcactcaaac
     1921 aatacaatca aaatctagca accactattt ttgacgacac acaaggctat ataagtgtag
     1981 cattcaaaaa ggacaagata gaaaagaacg gaaacgactt tcaattttta tgtttctatc
     2041 ctaattatga agtagccctt tatccagtcg tgacattact ttcttatctt gaaaagctac
     2101 cagaacaagc aaccgttgcg attaaagacc ctgacggtaa ctactcgtcc ctttatctaa
     2161 ccattgatga aacgatagat aaagggacga aatacaagtg gctagtaatt agcgacagac
     2221 tttcttatga acgcttttac ttaactagtc aattgactat acaagagcaa gcactggaaa
     2281 agatgttaaa agaactagaa accgaaccta acaatagact agaagccatt cacgactggc
     2341 tatgccaaca aaccgataaa gaccttttgg ttggtatttt aaaggaaaat agaacattga
     2401 aaggggcgga agcttactgc actcaaaagg cctatgaaat acgtaatggc aacggggcaa
     2461 tggctagtca tattactgtt tttaactggg ttagagaata ttatacgctt gacgaactgc
     2521 caacggataa gccaaaaata caagcgacaa taaaaagcga ttatatcaat caaaaaatac
     2581 agcgaccaac accagtagaa accaagaata ataacgaaat agatttgttt gagggggaag
     2641 atttttaatg aatactactg actatgtaga gaataaatta caaccgccaa gagcgttctt
     2701 tgaatggtgc tactcttctt ttcggacata tgtatggaaa aataaaaatg aaaccattgt
     2761 ttcttcaaca agaaaacacg attggataat tgagaaaaaa ttgagaaaga actctcgatt
     2821 gaccttttat gattcaagca gttgttttca gattatacta agtacaagta agcgaattga
     2881 agtacagacc tacaaagtta tttcagagta tgaaaatgga gtgcaatgtt ttcgtgaaca
     2941 gctagaatgt attgaaatat tttcaaataa tcaacatatc aagattggaa agatatgttt
     3001 acctgtatat tatggttaca atatgggcat agccttatat cctaatgaat ggaaaaagcg
     3061 tttagaacgt gtgtcagagt tgaaatatct aaatttagag cgattaaacg ttgataattt
     3121 agctaccacg tacaaatata gaaccttgat agaatttgct cagaaaataa atgctcataa
     3181 attagcatat gatgttatga gtggtgcagt agacatgcga atacttacaa agaactgttt
     3241 aaggaagtac aaaacatttt taaagaatac agacaatagt ctaaaagaga tacagctaaa
     3301 acaaacattt gaaagcttaa acataccaat ggtaaaaggg attgaaaaat acgtattaaa
     3361 gagcgacata tccgattttc caaatgagat tggcgctgtt aagtttcaga attggttagt
     3421 aaagcaagga aagtcattta agtattatca agactacctc aatatgttaa cgctcctaaa
     3481 aatagaaata aacaaacgta atcagttacc gccagattta gaagtcgcac acgattgtgc
     3541 agtagataga attaatcagt tgaactatga aaaaagggat aaagaaataa atgaacgttt
     3601 gaaacaacta aggaagtatg aacgagatat tgatgggtac acatttgtgt tacctaaacg
     3661 agcaaacgac ataaaaaaag aaggaaaagc actaaaccat tgcgtagcaa gctatataag
     3721 cagacacgca aagggagaaa caacgattat cttcgtaaga gagaagaaga accctcaaaa
     3781 atcttatttc acattagagt acaactacaa tagggttgtt cagttacaag gaaagaagaa
     3841 tcgacaaaag gtaccagatg aattgaaaca agcggtagac aaatgggtca aggtcattaa
     3901 agattaaacg ttaggaatgc taagaaaaga ccgttcttag cattcctaac cgaaatctag
     3961 gacaaaatcc attgttccgc ggcggtgcag tcgcccttgc tccaaaacca atggattttc
     4021 tgctgcactc aacacgagtc aattaaacgt tctattccca ttcgttccga ctggtgaacg
     4081 tttctctttt cctactcgtt cctcgcttaa cggaaaaggg cattgacacg agttgttcct
     4141 tgtgagacca caaggaagcg ttccgcagat ttaagttttt tctgaagcag tttcactgct
     4201 acagaaaaaa ggcgttggtt ctatggcatt tttctgtcac tcggcccctt cttgcggtta
     4261 tcatttcttc agtgagccgg ccttgtgtac ttgcgaatca tacaacagcc agtcgaaaac
     4321 acaagggtaa aatgcacgag caagctcgcc cttgtgtttt ctttgtgtct gatgtctgtt
     4381 ctctgcgcac ccaaaacctc aaactcactg aagaaatgga tcataacagc aaacaaagaa
     4441 aggagaggac cacagaaaaa taccgaacca acgaagttca aaagaagcac cccttcctaa
     4501 atcttcaaaa aaaatgagtt ttgaaaaaaa taaaaaacag aaagaagcga ataaaaatga
     4561 ctagacttga acaagcacaa ggaaaattgc aacgactaaa aagagaaagc gaagaaatgc
     4621 accacttaat cagggcggaa catgaccgta tcccttttgg acagcctaac attattggcc
     4681 gtggggacat ttataagaaa gtaaatggct atcatgatcg tgcaattaaa ttactgaagg
     4741 aacaagaaaa acaagaaaaa agagtggaaa tgctggaaaa ggtagaagat tttaaggaaa
     4801 aaaacgagct aatcaaagac gttcatgtgg tcggtaaatc aagttatgcg acggttggcg
     4861 caaaaacaag tgtcaataac attgattact ttaaaaatga actaaaagag cttgaaaagg
     4921 ctaacgaaaa agcaaaagcc tataacaaga caaaaccagc aatcaaagcg agaacgttcg
     4981 gcgcagtaat cacgaaacta aaaaataaaa ttgcgaccct ggaacaaatg aaggaagctg
     5041 atgaaaacaa ggtggtgagt gagagaacaa aagagctgat agaaagcggt gcagtgacgc
     5101 agtggaagaa aaaaccaata ttctatttcg ttaaagggtt gcgaaaagta gcactagaaa
     5161 ttgacgaaaa tggagaattc ttcatttcta actattatcc ggcttgtaca gatgcagata
     5221 aggaatttat cagtaagtta ttagatcctg cagctgaaag cagaaaaaaa gaaacctttt
     5281 gctgaagaca aaagatttct aggacgttaa ctatttatag tcatgaataa gtatagcgta
     5341 ccttagaaaa atattcaatg ggttcctgcc ataacaatgg agttatggca aggattcatt
     5401 gccactcata gaatagaaca attatctaac agtaacgaat taaaaaaaat attgcttttg
     5461 gaggaataca aatgagaaga ttaagtaata cgacaatggc caacgaaact ctaaaaaaag
     5521 aagcatggta tagaatgatg cttaccgacc tatcaagttc tgtcattgat gagttcatgg
     5581 aaacggggaa atgccattac accagcaatt attttcaagg tgaaaatatt ttagtcactg
     5641 aagaaataga ggcaatcata aagacggtcg aaaagaagta tggcttcctg gtgtactatg
     5701 taactgaaaa taaaacagcg gatggccaga gatttttatc attattctac gttggacgcg
     5761 atactagtga ttggctttac tgtcacaggg atttagaatc ttataggcaa tatgtgtatg
     5821 tagttaatac gacgaaccct gcattttcag aatttggaat gattcaattt gatccaatac
     5881 taggcagtct tttacgaact gcatagataa aaaaatatac attttatggt atcagttggg
     5941 ctttttggcc cagctgaagc ctaacgtaag gaattataag aaaggtggga aatgagtatg
     6001 gaaatacggg aattaattat aatagaagat ccttgctttt atcaatatac gacgataaaa
     6061 gaacttgaat cgtttaatac gtctgaatta acgattgctc ttagtgtgct tagaaaagag
     6121 cagcgtcgct tgaaagaaaa agtgtatcat tctggatctg gcgatcaagt ggataaaaat
     6181 aacgttaaat taatcaacaa gtacagagcc aatcttgaaa aactgctgtt tattaagcaa
     6241 gggtatattc cggatgcaat cacgcaaaac atgattgata cattgatcaa aaacaataaa
     6301 aaatccatac agaagattca aaaaggttga gtttagcaag gcaacaaaaa acatttaata
     6361 aaagagagga tgtaaaacat ggccgaaaaa attgatttga ataagttata tcatgaaagt
     6421 aaaaaggaaa gaaccgaaaa acaaacaaaa atggaacgag cgttagaagg agttgtgtgt
     6481 gggtttccaa gaaacacaga tgatgatcca attttaaaga atagtaagac ccaggagaat
     6541 accaatggat gaaaaaagga aagataaaat tcaacggttg ttagcactag cgaacgatgc
     6601 gaacgatgaa gaatcaatga cagctttagc aaaggcccag gaattgatgc tagcctatgg
     6661 aattgatgaa gaagaagtgt ttcattatca gcaacaccaa aggcaagaaa aggttataga
     6721 taaagtgatt tacaaaggaa gaccgcataa atgggtctac agactagctg ggatcattgc
     6781 aaagaatttt cgtgtgaaga attactatca aagttcttca gatatagaat tacactacgt
     6841 aggtttaaca acagatgtag agattgcaga aattacattt ttatatgcaa aaggtagtgt
     6901 tgccttttgc tcaaaagagt ttatgaaaca accggaaatc aagcgaaaat ataaacgaaa
     6961 atggcaatta aaacaagact atatcgaagg gtatttgacg gctcttagtt cagtatttga
     7021 agcccaggta cttacgaatg gttacgaact tgcattgcag ttgcctgtcg cagtccatga
     7081 agaagtctca aagctaggct tggttaaagg aaaagacacc tcacaccatg ttgaaaactt
     7141 cgatgctttt cattctggtt accaggaagg cttacagttt aagagtaaag agttaatcaa
     7201 ctaataaaat tattaatgga ggaaagagta tgcctaattt ggattcctac ttagaaagat
     7261 ttgaaaacta tcagaaggaa caagaagaat taaatgaaat ttttgatccg gacgatagga
     7321 ggtgcagagt ttgtggatgt actcaattta atgcatgccc agggggttgt tattggattg
     7381 aagaagactt atgcagtaaa tgtgtataga aagagaacaa aaaaagcaga taaaaaatta
     7441 tttgcttttt ttacttatat attttatacg ataaaaaaac gtggttttta gtttgaaaac
     7501 cctgtaagat taatatatgg acccatttaa tgtaaacata aaaggtgttc gttttcttag
     7561 gggggtattt taataaaaat tgaggagtga aaaaatgaat aagtttcaaa gttggcttat
     7621 ggccaataaa aatctgttaa ttacaagtgt agtaatgtgt caagtgttag tcttaagcat
     7681 agggataatc aactttcctt atattgatga caccggcagg caaattgatg gcaatacgga
     7741 ctttgctaga agttattctc gctggggtag cgaaattggt tcttggttag ttcaagggag
     7801 tagacacctt acggatatgg ggttgtggac gcatattctt tctggattca ttgtatcagt
     7861 aacagggatt atcattgttt actcttttca taagaagcta tctcttttag ctttaacagc
     7921 agccatgttg gttggcttta atccttggtt tttacaaaat ttaagtttca ggttcgatag
     7981 tccttacatg tctttgagcc ttttattttc agttcttcca tttctatttt ggaataaaaa
     8041 taaagtctta tttttagttg tttctatttt aagcattttt ttgatgtgta atacttatca
     8101 ggcgtcttcg gggatctacg tcgtgatggt gttagcatta tcactgaagc agctgttaga
     8161 caatcaatcg tttattgctg tgcttaaaaa agcgattgta gctatgatta gctatataag
     8221 cgccatgttt ctttatctta ttgaaacaaa atttaatcct gaaatagcaa cgcgaggcgg
     8281 tatgacaaca atcgcgagcg ttaaggatat acccaaaaca atcctggtaa atagtcaaat
     8341 gtatctgtca aaaataaccg agcagagtac aaagctatgg atacttttgt tttttattct
     8401 agtaattttc tttgttttaa gtacggttct taatgcgaaa gtatcgcctg ttaaaagctt
     8461 tttatacgca gtattatttc tatttttagg ctctattttg agttacggcg tctttctaat
     8521 ctttccggaa aagcttttgt taatttcgcc tagatacggc tatggattta gtttttttgt
     8581 agtttcaatt atcatgctta ctttgtcaaa tacttctaaa aacgtgctag gatatgtgtc
     8641 aaaaacagcg gtttgtcttt tctgtgtcta ccttttatct tttacatttg tttatgctag
     8701 tgcgttaagc caccaaaagg aatcttttga acgacaaagt atgatattag ccgatgattt
     8761 aaaagaccta gtgaaccgtg acacggtgac cgtgcatagt acgtctttat ttaaagattc
     8821 gccagtattt gctaatagtt cgaagaatta tcctatatta aaagaactgg ttccaccaaa
     8881 tgaagccctt tattggccaa atcagttttt atttagaacg tacactggtt taaatgtgaa
     8941 catggagatc tttgatatta acgcgttgaa taaagaagaa agtgagttaa tgaaatctaa
     9001 ttattaccat gacatttatg taaaagattc agaagtattt gttcatgtta aataatcctg
     9061 aaataaaagt aatatcaagg caattgaaag gccggggtag atactgtaaa ataaaaatag
     9121 ctatttcaga tttaactatt tacttacaga ctgaataaat tgagcgttca gttctatgag
     9181 tacttgttag aaacgaaagg ctaaaataag ttataacctt agagactgtc tccccccaag
     9241 agacagtctt atatattgtt taatagataa ttactttact aaaataggat aaattcaatc
     9301 agaaatgtat actttgtaag gaaaaaatca tagctgtttt aggatattat ttgctatttt
     9361 atgtatttgc caattacaat ggtattgtaa ataaatacat aaagtagcat tagtaggttt
     9421 tgggggatat agatttggaa aatatttttg atttaaacaa gggattatca agaagaaaat
     9481 attggtatta tcagtgtata ctgtttgtgt ttggattagg gatagaaact ttttcaatgt
     9541 ttgtatacaa aaagtctctg attgaaaaca attatttaaa atatttcgtg ataaatggtc
     9601 catttgattt ttataatttt cttaggatcg tgttctttat aatgattatt ataggttttc
     9661 tgttccaagc aagaaggttt gttgatattg ggtggtcgaa atttgtagct ttactaagtc
     9721 tgttaccgtt agttggtatt atcacaacag ggatttgttt atttaaaaag ggaaaagaca
     9781 ataaaacaga tagcagtcaa gtttcttgat tgctatctgt tttatttatt aagagtattg
     9841 tctattaaat gaatgacaag aacttgcatt gtaagcagct tgaatcgcag ctgaccatga
     9901 gagtttatgg aatgctactg cagaccaaat tcctttcata atacaagcac cttgttttgc
     9961 tttagcttgt tgatatttat tccataaatg tttagggact ttactttttg caaagttgtc
    10021 ctaataacca tcgtcacttt gggcatatgc agtagtaaag tttactaacg gataatacat
    10081 gataaattta aaaggtaggg tactttttcg tacaagaatg attttgtaag tatgaaacaa
    10141 gctagttata tttcactagc tattcaatga aataagtttt taaagtagat tgttgataga
    10201 tatattaaaa
//
LOCUS       DAJSMI010000090        10147 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJSMI010000090 DAJSMI010000000
VERSION     DAJSMI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.090"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            317..523
                     /locus_tag="ON430_001829"
     CDS             317..523
                     /locus_tag="ON430_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCR3219743.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(725..1726)
                     /locus_tag="ON430_001830"
     CDS             complement(725..1726)
                     /locus_tag="ON430_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3219744.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1731..3644)
                     /locus_tag="ON430_001831"
     CDS             complement(1731..3644)
                     /locus_tag="ON430_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR3219745.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3812..4318)
                     /locus_tag="ON430_001832"
     CDS             complement(3812..4318)
                     /locus_tag="ON430_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3219746.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4478..4918
                     /locus_tag="ON430_001833"
     CDS             4478..4918
                     /locus_tag="ON430_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR3219747.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4944..6101)
                     /locus_tag="ON430_001834"
     CDS             complement(4944..6101)
                     /locus_tag="ON430_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3219748.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6104..6460)
                     /locus_tag="ON430_001835"
     CDS             complement(6104..6460)
                     /locus_tag="ON430_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219749.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6758..7732)
                     /gene="sgrA"
                     /locus_tag="ON430_001836"
     CDS             complement(6758..7732)
                     /gene="sgrA"
                     /locus_tag="ON430_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCR3219750.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7928..8767)
                     /locus_tag="ON430_001837"
     CDS             complement(7928..8767)
                     /locus_tag="ON430_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3219751.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8952..9839
                     /locus_tag="ON430_001838"
     CDS             8952..9839
                     /locus_tag="ON430_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR3219752.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         3063 a         2022 c         1521 g         3541 t
ORIGIN      
        1 atttgtaatt ttatttcaca gttgatatga ttgttatgta aaataaaaaa gtttaggata
       61 catcgaattt ttggggaaat tcttttgggg gtatcctaaa tcttttctat tatattccca
      121 tattttctat tattggtcta gacgtatgat ggtaaacttt gtacctatct gattgctgaa
      181 tcattaattg cttatcatta gccatctttg tttcaagttc gctagatgat cattttattc
      241 acatcctaac aaacttgtca catttataga ttcttaatct atttatcata caattataaa
      301 aaaggaggtg ctgcttatga aagctatcca tatttctttg ctttttttcg ctagtgcatt
      361 gattttattt gctattcatc tttgttccca gccttatatc aataacaacg gaatattagt
      421 ggaaccagca ttcttttgct taccactcgg ctttttgtcc cttcttgcct ccgcaggctt
      481 cggatttgtt gcttttttta aacgatcaaa acaacaaatc tgacttataa aatttacgta
      541 aaagagatcg tgacaataat cgtgtgatat tcccaaattt cgaataaaag gtgccccttg
      601 ccatctctaa ctcctcagtc acaagttaca atattcataa accatgaaaa gcttgtttat
      661 tgaaattgct ccttgtgact tggagctaag tgacagatta caacctcttt tgcatcataa
      721 gactttactc cttgaaaaac attactaact tttgatatgt ttcttgcgct tgtttattgc
      781 tgaaatcaaa ctgatattca tggttcaaaa cagcattttt ctccgcataa tctacttttg
      841 tgactttcac ccccgctttc tcaaatacag aagttacttc ttccatttgt ttcgtaaacg
      901 tatttgtatt tccatccgtt aagaatgtgt tttgcgtcca acgatccgca tgtctgacca
      961 aggctaaact tttgaccttc tctgattgtt caaagtcctt attatcaaag taagcccatc
     1021 cacatttttc ataaagaaag ctagagattt tatcacttac ttgagtaagt gcagaaggat
     1081 ccaacagacc agacaaagaa ataaacttcg taatctgctt gttctccaaa gtcggatcaa
     1141 gatgatcagc ttttttaatt tcttcatttt gaagtgctaa caaatattct ccactaattt
     1201 gtgcacctgc cgaatttcca ccaatcgaaa tattcgacca atctgcttga tcttttatct
     1261 cttttttagc tgcttgaatg gcttcgttca attggataac tggtgtagga tgttttgctt
     1321 ccggtgatcg ttcataatct atatttacga tgatttgctg cgtgtctgaa catagatatt
     1381 ccaaataatc acgacaatca tttttatccc ctcctacata tgcgcctcca tgcatccaga
     1441 aaagaatcgg taattttttc ttttccactt tcggtgtgta gatatccata ttattatttt
     1501 tgtactttga aggataggat atttcttttt ttatatcgat ttgttcgatt ttttgctgga
     1561 aatcatctgg tcgggcatat acttgttctt tttcacttgt gtcaaattgt tttctaacaa
     1621 gcaagctaat cggctttggt gtgatttgat taatcaaaaa tacagtgact aaaataacta
     1681 cgatgataac tggtactgca atcactattc gcttgaacca tttcattcat ttaccctctc
     1741 tttcttcgcc aaatccatgt gataatcgtg ctaacagata aaaatccacc tgaataaatg
     1801 agaagcgaat gtttcttttc tccagttttc ggcaacattc ttttttttgc atctgtcttt
     1861 ttttgtactt tcactgtcat ctgaatcggt tgcgtatcat cacttgattg atcctctcga
     1921 ggttcattct ttcccttatt gtctgcattc tctgtcggat cttgcttttt tggtggagct
     1981 actgggattt ttttgtaata gaaaataata gttggtatcg tctcattaaa aatcccttca
     2041 gtcggtcctt caacatttgc taattcgtat ccttcgaatg tcttttgctc tgtcatataa
     2101 ttttctccat aagctccgta aagctggatt tcgtcctggt ctttgagtag acttttattg
     2161 ttttcatcca catactttac gtttatctga tcatggaaca ctactttcaa aaagtccgtt
     2221 acatgtttcg tgtaggcagc atggtcaact tcctgtgcct ttccatgccc tgctccttct
     2281 actaataaaa gatctttata ccctcctgat aactcatata atttttgcgc gttagacaca
     2341 ggtactacat catctgctgt tccatggata atcaatttag gcaatgaatc tgacattcca
     2401 atcgtaggta gagcagcttt tgtatccaaa tgcatctcct gatcaaaata gtattgtttc
     2461 aaaaggccca taaataaatc ttcatgctct tgatccccaa tgatgtctaa ctgatttttt
     2521 atggcatccg gtagtgcggg ggctactgtc tggctgtacg taaaacgtag ttgttcatca
     2581 attgatgcaa atccgcaatt ttcaataatt cctttgacat tgacaggaat atctttcgct
     2641 aatacagaca tcatcgtagc tgctcccatc gaaccaccat aaagtaaaat cttttgagat
     2701 ggccgctctc gaacttcttg atttatccag taaaggacgt catctgactc atattgtcca
     2761 taggtcacat aatcgcctcc gctattacca gtcgctctgg aatcaacaaa taataaatta
     2821 tacccggcct tataaaaatc attgtattct tctgtaacga tcccgttatt ccaattccct
     2881 cgaaatccgc catggatcat gactgtttta tctgaacctt gatcgacata atatgcatat
     2941 acatccatca ttttgccatc ttgtttgttt agtacagttt gatgaagttg ttttgtattt
     3001 ggatagaagc ctgtgttttc tgtaggcgtt gtcccatcat ctgtacctcc aagcatcgac
     3061 atcaacacac tgttttttgc aaaagcgata tcgtacaata gataagtagc aatcagatgt
     3121 ttgataatct ctttatctac accataatca ttaacagcaa gctcagtgac taataactct
     3181 attaattttg acttgatgtc ttctgttggt ccattaagta tttctgtcat ctgtttaccg
     3241 aagtaattca aatattcttt accattgaaa ttttcacctt taccgatatc atcattttct
     3301 gtcccattct ctttgtcact tactttagca gaagaggatt cgtcgtttga catttttgta
     3361 gctgttgctt cttctgtttg ttggttatct gtggtagcgg tcgtttcttg atctgtgcta
     3421 gtaacagatg tctcaaccgt tgtttcagta tttgtttctg tttgtgaagt actcgatttc
     3481 gtttcatttc cagcatcagt agtttctgtg gtttctacct cccgagattc cgattcggaa
     3541 gaaatgctct ctccatatac tgtccattgc gggacgcttg tacttacagc aaataagatg
     3601 accaacgctc cctttaatac ttgttttgtt ctcaagctat tcactccttt ttatgtttct
     3661 aggagaatta tatgtaaacg caaacatttt ttatacaaaa aataaatggt ttatcccata
     3721 aaaatagaac aggattattt tctcatttta cgtaataaag cagaatggat aaaccagtat
     3781 ccattctgct ttttttaaac gaagaaacaa cttattgctc caaaaatgtc agaaatcgtt
     3841 tatcccaaat tttctcgtaa aaagcgattg tttcctttgc tttcataaaa ggattatcat
     3901 aagttgtcgt tgctcctcta cacatcataa gctggtatcc taatccatga gcaaacttca
     3961 cagtcgtatc tatacaatac tctgcttgtg ccccacataa ttcgatcgaa tgacagtcca
     4021 attcagttaa taaaacactc aaattagtct tataaaatga attcgcatgg gtttttctca
     4081 caaaataact gtgctcagga atagataatt ccgacaatag ctcccatggt tttgtttgtg
     4141 gcactaattg agcatcttcg tgttgaacga aaataactgg tttcccaatc gtttcgtatg
     4201 ttttgattct tttattcact aatgctgtca ctgtttctaa ttcgtgtatc ggcgaagaat
     4261 caaaacatac ccctttttgt aaatcgatca ctactattgc gtcggctgtg tttgacatac
     4321 ttgtgccttc tttctttttt cttgatttaa tagtatttct tatctagcta gttccattat
     4381 acgtgacaac attttcttat tttacacctc taaatggcac ttttcgacta tttcttcatg
     4441 ttattatttc ataaatactt agctattgga ggaatgtatg atgattaaat tgtatacagg
     4501 ttctggctgc tcgtcttcaa gacgcgcaaa acgttggctt atcgtccatg atataccgtt
     4561 tatcgaacag aacttaattc gtcaaccact aaccaaaaat gaatttatcc acttactaaa
     4621 attgacaaac aacggcgtga ccgaaatgct ctctactcat tctattagtt ataaaaatct
     4681 tgttcacaga atagatgaac tcagtttgag tgaactatat attgagattc gtacatcccc
     4741 ttctttattg aaaaaaccga ttatctacga ccaatctgca aaacttttga ctggatttag
     4801 cgaagataaa atcaggatgt tcctttctaa aactaaaaga aagcaagagc tcaacgaact
     4861 cttgctttct ggtcatcttg tcgattttaa agaaaataat ctttttgcaa tgtcttaatt
     4921 ttggcataaa caatctgctt ttcttaatta acgaaatcca atttttccaa tatggatcta
     4981 tttttctcat tcctgatttt tatgatttca gctttcagac gcgcccaatc attttttgta
     5041 ggttgtccat tccagtaaaa tatctgtgta ttcgtttgat catatgcctt tttgtcgata
     5101 ggataatcgc taatgacaat atcaggtaac tgatcagcgg atgcactata ttttattggg
     5161 cgttcagtca tagataatat ccttttcttt gcccataatt cctgtgtttt accatacttt
     5221 gattgaatat agaatatgac aggttgttcg tgttttataa aaatatccct gactaacata
     5281 cagtattgaa aaatcaaccg tctgttcttt tcataataag tattaagctc tggaattctg
     5341 cgagctaccc cactaaaaaa ctgttgcatg attggataca tcactggaaa tatatgctga
     5401 aaatctctgt cttctgctga ttttccaaaa gcatccacct tcttttttgt cccaaaaacc
     5461 cttgaataca aatggatcat tgccaaattg acatatagat aatttttttc tgaagttgta
     5521 aaacaaatct tgaagttttt ttcaaaattt tctacaatag acgattcaat ccagccccaa
     5581 ctttcttgta ctttatctgg agtatccaac aaatcttctt ccaaataggt gttcattaca
     5641 ccaaacataa aataaaggta agcttgctct gttggagtga tgtgattcat cgaccagaag
     5701 tttgcttcaa aaaaaggctc tagtatgtaa tgcgtaaact cttcttttgt tatcaatgga
     5761 tgcacaaatt tgttcatctc ttctgggaca tacgtaatcg agtggccttg tttcattcgt
     5821 tgcaacgaaa ttcttagtag gctgatccat ttctttctat ctatactacg cgtatatggc
     5881 gcatgtttcg caaatttttc taatagttga cgtttatttt ttatacttga taatatatca
     5941 gaatgatttt cgtcgaatga ctgccaatac atcaagtgaa aaaagtatcg gatacggtat
     6001 tcttctcctt ctatcggatt accttttttc aagctgcaag ataaatgata gtttttcaaa
     6061 atagtattaa gttctcttaa ttttctttga atcgtacgca aacttatata attttttgac
     6121 taagattttc taaggattga taagtacctt tgtaaagtga atccagtaaa atataaccaa
     6181 tcgttccctc taaaagtata gaataaactt tactcagtaa aaactgagaa gaaaactcga
     6241 cttttatcat cttgttttct gaaagaaaag ataatttata ttctggatac ctttcgcaaa
     6301 acgattctgc ttgttgaatc accgaattca tcttagccag ggtgtagttt agatctaaag
     6361 cgatatcctt caccacatat aatttctcgt ctctttcata acaatagttc aaaaattgat
     6421 atacttgcct ttcattgata tcaataaatt cttcaaacaa agtacatact cctttttatc
     6481 tattatttag acttattccg acagctacat gaataacgta caaatagatt cgttactgat
     6541 ttttcagaga tactaccatg cgcagtgtgc attgtcaatt attcttgctc ttattttcca
     6601 tttgtgttcc tgttatttcc tgtttctcaa atatgttagt aaacaaaaat agatctggca
     6661 aagctacaac ttcgccaggc aatacagcac tctcgaatga aatattcact caaaaatcta
     6721 tcgttttatt caattttaga tctgttgctt ttattcttta tttcactcga tggaagagag
     6781 aacgaaaata gcttgctcct acaatcaaaa tgagtaacac acccatccat gtaattagct
     6841 tgttcaaggt tctactacca gtttctggta aatcgctttt tctattatct gtttctttcg
     6901 ctttttcttg atgatcaatt ccacgagaaa caaaatcagg ctgttttagc acacttttta
     6961 ggttgctccc tgcatttctc ttttgttcct tagttggtat gataggtacc aaatcaagtt
     7021 cattatttac ttttcctgtg ctgctttcgc tgctcgtgct tcctgtgcta ctctcactac
     7081 tcatgcttcc tgtgctactt tcactactcg tactctccgt actgctttcg ctgctcgtgc
     7141 ttcctgtgct actttcacta ctcgtactct ccgtactgct ttcgctgctc gtgttttctg
     7201 tgctactttc actgctcgta ctctctgtac tgctttcacc gctcgtgttt tctgtactgc
     7261 tttcactact tgtgctatcc gtactgcttg gcggtccaac aactgtccaa ctgcttggtt
     7321 tcaaattctc cgtgatcgtc atctgtccat cataaaaaac aatcaaatca actttatctt
     7381 tactcttgat ctcatcaatc gatagttctg cagaaatcat tattgtatac tcaataccag
     7441 cggtccattg tacgtctttt aaataaactt ccccaataac tttttctgaa cttccattac
     7501 catcccaacc atatgttaga acagcatgta catcactagc attcacgctt ttgtttggtt
     7561 tgactggaat agtaatgact tgttgtgtca tgtcataaac aatccgatca gttttcacag
     7621 taaatgaaag attctcattt gcccgttcat ttgcagaagt actgattcca atcattgctg
     7681 caattatact cagcccaaga ataacaaata atacggtcgc tgttttcttc atttctatca
     7741 ctctccatat aattaccttc tatttgataa aggaattata taaaatgaaa atgatgagaa
     7801 cacgacagtc agttgtgaaa atctaacaaa agatgtcgtg tattcgcttg aatttgttta
     7861 ctttttcgtt taatcaatga cagacttaaa aaagcaagac cgatatcagc cttgctttct
     7921 tcatctttta ttgataaaca gcagtgtatt tcttcatccg ctttgtttta tatgcataga
     7981 gtaacaaacc aacaatcaag actaacaaga ataatgtagt tgatatagat gcttcagccc
     8041 caaagtttcc cccagtaatt attgtatttt gcgattggat cagagtggag aaaaaacctt
     8101 gttcttgcgt tccgctgact ggtataccca aaatagctcc ttgtgtccaa ttccaagtaa
     8161 tatgagcacc aatagccgta aataaacttc ctgtactgta gataagtgcg ctgaaaaagc
     8221 aacctgctaa aaagatattg aaaactgcca gaaatgtcat gttaggattc aatccgtgca
     8281 aaagagcaaa taatgcagca gatccaatca ttgctattaa cggaccaaat ctaagcatct
     8341 gatgctgaaa aaagccacgg aataaaagct cttctgatgc ccccactgca ataaataaaa
     8401 taaaactcgt tatcagcggc acaatgtttg cgtttcctga atctaaccga tacgcccccg
     8461 tcaaatataa tggactagta ataagcagaa acactccaat tccaacaacg agacctcccg
     8521 ctaaataccc ccagtctttt cctcgaacag aaacaccata atcttctatt ctttttcctt
     8581 cgaccttact agcaagaaat aaagctagta aaaaaacagc taaatgtccg accgtaccgg
     8641 caattgctcc aatccattca ctatttccaa taaacatatc cgcaagtgca ttattgacaa
     8701 gaattcctaa aacaaaacca actaagacta ttaaaacaga tccaatcaac cgaataatcc
     8761 tgatcatctt tcatccctcc tttatattaa gtttaacaac ccttttcttt cttgtctcgt
     8821 gaaaactatg taaatgttct ccatcataga gagaatagat ttttacgaac taagaatatc
     8881 tacagcaacc ttacgaaaat gaaagactgt ctccctaatt cttctgttag actgttttta
     8941 ataaggaaag gatgggaaat tttttgaaaa aacaactact aattatgtgc ctttcagcat
     9001 gtgcaatttt actattctca ggttgtagca agtccaatga aacgattgca acgtttaaag
     9061 gtggaacact tacagttcag gatttttatg ataacccaaa agttaaatca aataatcaaa
     9121 cactattaaa aaaaatgatt gtcttcaaag catttgatga tgtatacgga aaagagattt
     9181 cttcaaagga agtaacaaaa gaatataatg aacaaatcaa aaaactcggt ccaaactaca
     9241 aggaacaatt aaaagcagtt ggccaaacag aagagacata taagaaactc tttaaacaga
     9301 tgctggcttt ccaatacggt ttgaaagcca atgtaaaatt aacagataaa gatctagaca
     9361 ctgcttggaa agaattctat ccagaagtta gtacacagat cattctattt tcgactaaag
     9421 aagaagcaga taaagcaaaa aaagaagcaa atgaaggaga aaatttctcc aagcttgttc
     9481 aagcttatgg aaaaaataaa acattaaagg aaactgatgg aaaaatgaat tttgattcaa
     9541 ccaatccaga gattcctact gaagtaaaaa aagcagcatt taaactaaaa aacggggaag
     9601 tttctgatat cattcctgta acagatccaa cgacttatca gcaaagctat tatctagtaa
     9661 aaatggtaaa aaaacaggat aaaggttcta ataaagacaa atacaaatct gagctagaaa
     9721 aaattgctac cgaagcaaaa actcttgata cggaattcat ggataaaaca atccgtaaag
     9781 tgatgaaaaa agacaatgta actattaaag atccttatgt aaaaaatata ttcaaataaa
     9841 caaatttgac ccaattgggg atgtggcaaa atttgagaag tgatttttgg aaattccttc
     9901 ttatttttga ggcaaaccac ttttgccaca acttcatcgg ggcctttttt tatttgtacc
     9961 aaaacaaatc actggatttg ttacaatcat gaggatataa tttaaaaaag ttttttattt
    10021 ctatcacatg aatcaatgcg ccattaagta gattctttca tgcttcccta ttttttcact
    10081 tgccgtatcg cttcaaaaac aactatcata aactcaaact cgactgaaaa gttattttaa
    10141 aaaagac
//
LOCUS       DAJSMI010000091        10038 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJSMI010000091 DAJSMI010000000
VERSION     DAJSMI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.091"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            73..1011
                     /locus_tag="ON430_001839"
     CDS             73..1011
                     /locus_tag="ON430_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCR3219753.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            1026..1856
                     /locus_tag="ON430_001840"
     CDS             1026..1856
                     /locus_tag="ON430_001840"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR3219754.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            1900..2925
                     /locus_tag="ON430_001841"
     CDS             1900..2925
                     /locus_tag="ON430_001841"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR3219755.1"
                     /translation="MNYRVAVLGPGSWGTDLAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            2940..3878
                     /gene="galU"
                     /locus_tag="ON430_001842"
     CDS             2940..3878
                     /gene="galU"
                     /locus_tag="ON430_001842"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR3219756.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            4003..4443
                     /locus_tag="ON430_001843"
     CDS             4003..4443
                     /locus_tag="ON430_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCR3219757.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            4446..5048
                     /locus_tag="ON430_001844"
     CDS             4446..5048
                     /locus_tag="ON430_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3219758.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(5107..6213)
                     /locus_tag="ON430_001845"
     CDS             complement(5107..6213)
                     /locus_tag="ON430_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR3219759.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            6423..7424
                     /gene="ccpA"
                     /locus_tag="ON430_001846"
     CDS             6423..7424
                     /gene="ccpA"
                     /locus_tag="ON430_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCR3219760.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(7761..>10038)
                     /locus_tag="ON430_001847"
     CDS             complement(7761..>10038)
                     /locus_tag="ON430_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR3219761.1"
                     /translation="IFRVFQSLTVFITVLLLMFAALGIGIGAGYFAYLVEDTQLPTKK
                     ALQTELGNITETSKIVYADNTEISKIQTDLMRTTISSDKISPLLKTAIISTEDEYFDK
                     HQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQQILTDETTFKRKANEIILSAQVE
                     KYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAKGIFGVDAKDVTLPQAAYIAGLPQ
                     SPITYSPYTNTGALKEDLSAGLARKDFVLFSMYREGQITKEQYEEAKAYDLTKDFLPQ
                     QIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADMSDSSVSDAYYQKAQQTIQNKGYT
                     IHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETGNVLMDNRTGRIYGFVGGRNYSQN
                     QNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSESRVSDYATTWQEGENAGEKIVNAT
                     NEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGSKQYAYEHYLAKMNYPANDNWGVE
                     SAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDSITDNAGNVIYKHESNSVRIYSEA
                     TASIMNDMMRSVINAKITTPFKDAISSLNGNLGKADWVGKTGSTNEYRDSWLIVSTPS
                     ITISSWAGHDDNTGMDSKARIRSANYLANLINQAYQAKPDIFGTDEKFELSSDVMKKK
                     VSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGPKKSAFKFGVGGTDENYTDYWNTA
                     SAYARANQATKESDEKDD"
BASE COUNT         3151 a         1847 c         2127 g         2913 t
ORIGIN      
        1 taattggtag tacttttggc tagacaatga taaaatgata gagaacactc taatagaaat
       61 gaggtagcat gaatgtcaga agttgtaaaa gttagccaat tagtgaaaaa attatctttg
      121 gaaatcgtga caggcgacga gaaaagctta gaccgtgtca tcgtaactgg ggacatctca
      181 cgcccaggac tagagttgac tggttatttt aattattatt cccataatcg tttacagcta
      241 tttggcagta aagaaatcag ttttgcggaa cggatgatcc cagaagagcg tttgatcatc
      301 atgagaagat tatgtagtga agatatgccc gcatttatta tttcaagagg attaccagct
      361 ccagaagaaa tgatccaggc agcaaatgaa aatgggatag ctgtactgcg ttctcccatc
      421 tcaacttctc gtttattagg agaattatcc agttatttag atggtcgcct tgcccctcga
      481 acaagtgtcc acggtgtttt agtggatgtt tacggattag gtgtactgat tcaaggtgac
      541 agtgggatag gaaaaagtga aacagcgttg gagttgatca aacgcggcca tcgattgatc
      601 gcagacgatc gagtagatgt ttatcagcaa gatgaattaa cggtgatcgg agaaccgcct
      661 aaaatcctgg aacatctgat tgaaatccga ggaatcggga tcattgatgt aatgaatctt
      721 tttggtgcga gtgcggtacg tggctctatg caagtccagt tggctgttta tctggaagca
      781 tgggcaaaag ataagaaata cgaccgtctg ggctctgaag acacgaccgt tgaaatcgcg
      841 gaagtgggca ttccacaaat aaaaatccct gtcaaaacag gacgaaacgt tgcaatcatc
      901 attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg gctttgacgc aacgaaaaca
      961 tttgaagaaa gattaagtcg cttgattgaa gaaaactcag gcaacgacta gcaaggagtg
     1021 acaatatgtt agcacaaatc aatcgagttg cttttgagct tgtagggatt cctatctact
     1081 ggtatgcatt gatcatcgta tcaggtatca ttatcgctat gtggcttagc agcagagagg
     1141 ctgttcgggt aggattaaaa gcagatgatg tgacagattt tatgttgtgg ggattgccta
     1201 tctccattat tggcgctcgc ttatattatg ttttatttga tcttccccag tatatagctg
     1261 atccaatcca aatattcaat atccgttcag gaggcttagc tatttatggc ggactgattg
     1321 caggcgcgat cacgctgtat ttttttacta aatatcattt tatttctttg tggacttttt
     1381 tagatatcgc tgcaccaagt gtattattag cacaggcaat cggtcgttgg ggaaatttca
     1441 tgaaccacga agcatatgga ccagacacga cgcgaagctt cttagaaagt ctgcatttac
     1501 cgcaattcat cattgataat atgtatatcg aagggatcta tcgtcaacca acatttcttt
     1561 atgaatcact ttggagtttg attggattta ttttgctttt gtttttacga aagaaaccac
     1621 accttttgaa aaaaggcgaa gtccttttat gttatctaat gtggtatagt ttcgggcgtt
     1681 tctttattga aggattacgt acagacagtc tttacttttt aggtgtgatc cgtatttctc
     1741 aattattatc tgctgtgtta tttgtcggta cattgattct atttatttgg cgcagaaaaa
     1801 aaggaaattt accggattat gaccgctcat taggaaaaaa tcaacaaatt atctgatttt
     1861 tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa tgaattatag agtagcggtt
     1921 ttaggaccag gctcttgggg tactgatttg gcacaagtcc tcgccgaaaa cggacatgac
     1981 gttcggattt ggggacatcg tgcttcacaa gtagaagaaa tcaacaccca acacacgaat
     2041 caacgttatt taccagatca ttatctaccc gattccatcc aagcattcac caatatggaa
     2101 gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc caacgaaagc aatacgttct
     2161 gttgcaaaag aactagtgcc taaattgaat acaaagccag tgatcatcca tgcaagtaaa
     2221 ggattagaac agggaacaca taaaaggatc tctgacatct tgttagagga aatcccagca
     2281 gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc atgcagaaga agtagcaaaa
     2341 catgacatca ctacgatcac agctgcaagt acagatgcaa aagcagcaaa atatgtacaa
     2401 gagctgttca tgaataatta ttttcggatc tataccaatc ctgatgtgat cggtgtagag
     2461 atgggggcag ctttgaaaaa tattattgct attggtgctg gcgctattca cggcttaggg
     2521 tttggcgata atgcaaaggc agcaatcatg acgcgcggat tagctgagat cagccgttta
     2581 ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat taagcggaat aggcgatcta
     2641 gtcgttactt gcacaagtgt tcattctagg aactggcgcg caggcaactt gttaggacaa
     2701 gggcagcccc tagaagaggt gctagccaat atgggaatgg tcgttgaagg tgtgaatact
     2761 acccaagcag cgatggaact agcccaaatg atggaaatcg aaatgcccat cactcaaaca
     2821 atctatgaag tgctgtatga acaaaaagaa atcaaaacag ccgcaaaaga aatcatgtta
     2881 cgtgatggaa aaaaagaaaa tgaattctca ttaacaattt tttgaaggag cgaatgtata
     2941 tgaaagttaa aaaagcagtt attccagcag caggattagg aacaagattt ttaccagcaa
     3001 caaaagctat ggcaaaggaa atgttgccaa ttgttgataa accaacaatc cagttcatcg
     3061 ttgaagaagc gttgaaatca ggtatcgaag acatcttgat cgttacagga aaagctaaac
     3121 gaccaatcga agatcacttt gattcaaata ttgaattaga aacaaaccta agtgaaaaag
     3181 gaaagaccga attattgaaa ttagtagaag aaacgacaga tgtcaacttg catttcatcc
     3241 gtcaatctca tccaaagggt ttaggacatg ccgttcttca agcaagagcc tttattggaa
     3301 atgaaccatt tgtcgttatg ctaggcgatg atataatgga agacgaagta ccattgacac
     3361 agcaattaat cgatgattac gaacgtactc atgcttctac gatcgccgtt atgcaagttc
     3421 ctcatgatga aacttcaaaa tacggaatta tcaatccagg agaagtattg gaagatgggt
     3481 tatacaatgt caaaaacttt gttgaaaaac cagatccaag caaagcacca agtgatttag
     3541 ctattattgg acgttactta ttgactccag aaatttttga tgtattgtcc gagcaacaac
     3601 caggagccgg aaatgaaatc caactaactg acgcaatcga tacattgaat aaaacacaac
     3661 gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt cggtgacaaa tttggtttta
     3721 tgaaaacaag tattgaatat ggtctaacac atccagaagt aggtgcacct ttacgtgagt
     3781 acatcctgtc tttaggtaaa acattagaag cagaaggaag caagatcgat cataatccaa
     3841 aaggaataac agaaatcaaa gaagaaaaga aaaaataacc cttttttcta acgagcttca
     3901 gctgctaatt gaataaaaag tagttgaagc tcgtttttat ttgtttaaaa aacacgaaaa
     3961 tgctatcctt atagaaaaga gagacaagaa gggagtttga tcatgtcagg aggagaaatt
     4021 gcaggattga tcgcagcaat cgcctttgcg atacttgtcg tttttatcgt tcgtgtgttg
     4081 ttgcaagttt ctaaaacttt agaaaaagta gatcagactg tagcagaagc aaatacgacg
     4141 atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac aagtagaagg cttactggtc
     4201 aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg caacgatcga tccattgttc
     4261 acagcagttg ctgatttaag tgaaagtgta tcagaactta atcattctag taaaaacatc
     4321 gcgacgaaag tcggcggaat cggtaaaacg acagctaaag cgacggttgc cggaaaagtc
     4381 ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata ctgaaacgac aggaggaaaa
     4441 taaaaatggc taaaaaaggt ggatttttat taggtgcagt agtcggaggc acagccgcag
     4501 caatcgccgc gttattgtta gcaccaaaat ctggtaaaga acttcgggat gaattagctg
     4561 cccaagcgga tgatttgatg gatatggctt cagattacac agatatggcg aaagaaaaat
     4621 cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga tctttctcaa caagcaggtg
     4681 atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc agatgaagca ggaagtgaat
     4741 ctgacgatat cttgttaaat cttaaagaac aatcagctga cttatctaat cgtttcaaaa
     4801 aaacggcaga agatatgaaa aaacctgcag atgaattagg aagtattgct aaagatactt
     4861 cagaagatat catgattgat gtaaaagacg ctgctggtaa agcaggagat actgtcaatg
     4921 aaggaatgtc agaagcaaaa ccagttgcag atgactcaaa agaaatgatg aaaatggcaa
     4981 aagaagatgc gaaagaagct aaaaaagaaa taaaagatac aatgacagat tcatctacaa
     5041 atcactaaaa tcacaaattt atagaataaa aaggtgggag cggactagct ctcacctttt
     5101 ttactcttat ttttctaaaa caagtaattc ttttggtgta gtcgtgaatg gcacacagcc
     5161 atcgcttgtc acatatacac agtcttcgat acgtacccct actttttcag gaatatagat
     5221 tcctggttca agagagaagc acatcccttc ttcgattact aagtcatttc cttctacaag
     5281 ggaagggaat tcgtggacgg ttgttccgat cccgtgacct aaacggtgat tgaaatattc
     5341 gccgtagcca tagctgttga tcacgtttcg tgcgatttga tcaagttcac ctgctgtcac
     5401 acctggacgg acggcttctt gtgcggcaag ctgtgcttca agagtgatgt tgtagatttt
     5461 ttcttggaag tcggttggtt tttgataagc aattgtacga gtagcatcac tgcaatagcc
     5521 attccaaaca acgccaagat cgaataacac aagttcattc ggtgaaatct tcgtatttcc
     5581 tggtgtacca tgagggcttg cagcatttgc tcctgcaagg actaatgtgt cgaaagacat
     5641 ttgtgaaaca ccttgttttt tcaattggta ttcaatttct gcaatgatcg cttgttcagt
     5701 cacgccttct ttcactgctt taaagccaat ctcaaatgca acatcggccc atttaccagc
     5761 ttcgatcagt ttgtcttttt cctctagagt tttgatcagt tgtagacgtt ggatgatgga
     5821 agttagatca gtgtaagaat ccgttcccgt caagatatca gagatagcat cgaagcgctc
     5881 gacagtcaag gcgttctttt caatccctac attgccaggc tgaccatagc gagcacgaag
     5941 ttcttttccg atgatctccc aagggttttc gctgtctaaa tagccacgga catcatacgc
     6001 ccaactactg ttttgagcat cttcaacttc caaagcagga gtgaaaagga aaggatcttg
     6061 ttcaagaggg ataaaaagag ctagtacacg ctcatgagga tcactttcgt atccggaaaa
     6121 gtaagcgatg tggccagggt cgctgatata agccaattcg acttggtgtt gtgccatcca
     6181 ttgacgtagt tcattgattt ttacgttatt catttggcaa cctactttct aaattaattt
     6241 tgtattcaat aattgtacca ctgatttatt aaaagaccaa cgttctaaaa actgaaaaaa
     6301 gtaaaaattt tcatgaaaac agagataaaa cggttgcaat tcttgagatt atttgatatt
     6361 ctattttaga aatgtaagac gtattttcag aaaacatata aaatatgaat aggagaacac
     6421 aaatggaaaa gcaaacaatc actatctatg atgtagcgag agaggcaaat gtttcgatgg
     6481 ctactgtttc acgtgtcgta aatggcaatc caaacgttaa accagcaaca agaaaaaaag
     6541 tcttggaagt tatcgatcgc ttggattatc gtccaaacgc agttgcgcgt ggcttggcaa
     6601 gtaagaaaac aacaacagtt ggtgtaatca tccctgatgt cagcaatatg ttctttgctt
     6661 ctttagcacg tggtatcgat gatgtggcaa caatgtacaa atacaatatt atcttagcaa
     6721 attcagatgg caacgaccaa aaagaagtca atgtattgaa taacttactg gctaagcaag
     6781 tggacggcgt gatcttcatg ggtcaccata tcacagacga aatccgtgga gaattttcac
     6841 gttcaaaaac tcctgttgta ttagctggct ctattgatcc agatgaacaa gtaggtagcg
     6901 taaacattga ttatgtatcc gcaacaaaag atgcagtaaa caaattggca aaaagcggca
     6961 ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc catcaatgga caaaatcgtc
     7021 tgaaaggcta taaagaagca ttaagcgaaa ataacctacc atatagcgaa ggattgatct
     7081 ttgaagcaca atacaacttc aaagatggtc tatctctagt cgatcggatc cataatagcg
     7141 gtgcaacagc tgtctatgtt tcagatgatg aattagccat cggtattttg gatggtttat
     7201 tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat cacaagtaac aattcactac
     7261 tgacagaagt agctcgtcct cgtttaacaa gtatcacaca accattatac gatttagggg
     7321 cggtatctat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa gaaaaaacga
     7381 ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa ataataaaag gacaaccatt
     7441 cagaggttgt gtgcctgccg tttaactctg cgttacaagg agcaatttca aaaaacagca
     7501 tctcatgttt gatgaatatt gtgactcgtg attcggagtt ggcaggagaa caccgtttat
     7561 ttgggaaaag gggttatgac aaaattttgt tataatcctt tttttgtgtc ttaaaaaaga
     7621 gagaaagtgg ttgaaattac atttatcgta tataaaacaa tccgtgtata gatgctgcat
     7681 tttgttcacc accatcaaaa aaataaggga ctgtgacaat actattgtca caatccctta
     7741 ttactgtgta ttctattaaa tcaatcatct ttctcatctg attctttagt tgcttgattc
     7801 gctcttgcat aagctgaagc ggtgttccag taatctgtat agttttcatc ggtaccgcca
     7861 acgccaaatt taaaggcgct tttcttcggt ccatttttcg cccatagcga ttcaactgtt
     7921 tttcctggtg tttggatgga ttttttattg acagtaactt ttccaggtgt ttgaccagta
     7981 aatccagata cttttttctt catcacatca gatgataatt cgaatttctc atcagtgcca
     8041 aaaatgtcag gttttgcttg gtaagcttga ttgatcagat tggcaagata attagccgaa
     8101 cgtatccttg ctttgctgtc cattccagta ttgtcatcgt gccctgccca gctactgatg
     8161 gtaatagacg gagtagagac aattagccat gaatctcggt attcattggt agaaccagtt
     8221 tttccgaccc aatctgcttt tcctagattt ccgtttaagc ttgagatggc atctttgaat
     8281 ggtgtagtga tttttgcatt gatcacacta cgcatcatgt cattcatgat cgatgccgtt
     8341 gcttccgaat agatccggac agagttgctt tcgtgtttat aaattacatt tcctgcgtta
     8401 tctgtgatgg aatcgatgat atagccttct tcatagacgc cgccgtttgc taaagcctga
     8461 aatccgttgg tctgttgaag cgtcgtgaca tctactgtac ctaaaggagc agattcaacg
     8521 ccccaattgt catttgccgg gtagttcatc tttgccaagt agtgctcgta agcatattgt
     8581 ttcgatccac cggaattcaa cacatcttga tataagtgat aagccgggat attgttggac
     8641 cattctagtg actcacggac ggtttggaag gtgttcgatc cttcgttagt agcattgacg
     8701 attttctcgc cagcattttc tccttcttgc caagttgttg cataatctga tacacgggat
     8761 tctgatccga taagccccat gtcgattgca ggtccataaa caagaactgg tttgattgat
     8821 gagccagctt gacgttctgt atcgaaggca tggttatttt gattctgact gtaattccgt
     8881 ccgccaacga atccgtagat gcggccagtt ctgttatcca tcaatacatt tccggtttct
     8941 acttgactgc cagcaccatc atctaacatg tatccataat tttcaacgcc attttgcaat
     9001 gcagcataga catctttatc gattgtcgaa tggattgtat atcctttatt ttggatcgtt
     9061 tgctgtgcct tttgataata agcgtcactg accgatgaat cagacatatc agcgttgtct
     9121 ttttcagcca attgttgtgc aatgattctt gttgcttcat tcatcacagt ataatacaag
     9181 aattctctgt cagtctgttc tgcgatttgt tgtggtagaa agtcttttgt aaggtcatag
     9241 gcttttgctt cttcgtattg ttctttcgta atttggcctt cacgatacat actgaataaa
     9301 acaaaatctt ttctggcaag gccggcagaa agatcttctt ttagcgcgcc tgtgttcgtg
     9361 tatggactat acgtgatcgg gctttggggt aatccagcga tataagcagc ttgcggcagc
     9421 gtcacatctt ttgcatctac accaaagatt ccttttgcag cttcctggac tccagcaatg
     9481 ttttgtccct tattattacg accaaatggt gagacattca aatacgtagc aatgatctca
     9541 tcttttgaaa agtatttttc tacttgtgca gaaagaatga tctcgtttgc tttacgtttg
     9601 aatgttgttt catctgtaag gatctgctgt ttgactaatt gctgtgtcaa agttgatcca
     9661 ccagaagagc caataccagt agcttcagat actaatgctc gtaaaacagc tttaggaacg
     9721 taaccttggt gtttatcgaa gtattcatcc tctgtgctga taattgcagt tttcaataaa
     9781 ggtgaaatct tatcagatga aatagtcgtg cgcatcagat ctgtctggat ttttgatatc
     9841 tctgtattat ctgcataaac aatctttgac gtttctgtaa tgtttcctag ttctgtctgc
     9901 agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat aggcaaaata gccagcgcct
     9961 attccaatac ctagagctgc gaacatcaaa agaagaacag taataaaaac agtcaatgat
    10021 tgaaaaactc taaaaata
//
LOCUS       DAJSMI010000092         9931 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJSMI010000092 DAJSMI010000000
VERSION     DAJSMI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.093"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            129..1073
                     /gene="mvk"
                     /locus_tag="ON430_001848"
     CDS             129..1073
                     /gene="mvk"
                     /locus_tag="ON430_001848"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCR3219762.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1066..2043
                     /gene="mvaD"
                     /locus_tag="ON430_001849"
     CDS             1066..2043
                     /gene="mvaD"
                     /locus_tag="ON430_001849"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCR3219763.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2056..3141
                     /locus_tag="ON430_001850"
     CDS             2056..3141
                     /locus_tag="ON430_001850"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCR3219764.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3138..4202
                     /locus_tag="ON430_001851"
     CDS             3138..4202
                     /locus_tag="ON430_001851"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR3219765.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4451..5254)
                     /locus_tag="ON430_001852"
     CDS             complement(4451..5254)
                     /locus_tag="ON430_001852"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR3219766.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5274..6116)
                     /gene="kduI"
                     /locus_tag="ON430_001853"
     CDS             complement(5274..6116)
                     /gene="kduI"
                     /locus_tag="ON430_001853"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR3219767.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(6132..7139)
                     /locus_tag="ON430_001854"
     CDS             complement(6132..7139)
                     /locus_tag="ON430_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR3219768.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(7146..7790)
                     /locus_tag="ON430_001855"
     CDS             complement(7146..7790)
                     /locus_tag="ON430_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR3219769.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(7890..8648)
                     /locus_tag="ON430_001856"
     CDS             complement(7890..8648)
                     /locus_tag="ON430_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR3219770.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(8775..9926)
                     /gene="fucO"
                     /locus_tag="ON430_001857"
     CDS             complement(8775..9926)
                     /gene="fucO"
                     /locus_tag="ON430_001857"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR3219771.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
BASE COUNT         2793 a         2163 c         1939 g         3036 t
ORIGIN      
        1 tagtaaaata aaggaggaat gttttttgag acccttagtg gacaaaaaaa ttcgtgaagc
       61 agcgcaactc gttttgggcg ctttattttt tgcttaaaaa tcacttttac gtgagaagga
      121 gttagattat ggcaaactat ggccaaggag aatcaagcgg aaagatcata ttgatgggcg
      181 agcacgcggt tgtttatgga gaaccagcga ttgcctttcc tttctatgca acaaaagtca
      241 ccgcattcct tgaagagctg gatgcaatgg acgatcaact ggtttcttcc tactattcag
      301 gaaatttagc cgaagctcct catgcattaa aaaatatcaa aaaattattc attcacttaa
      361 aaaaacagca tgacatccaa aaaaacttgc aactgaccat tgaaagcacg attcctgctg
      421 aacgtggaat gggatcaagc gctgcagtcg ccacagcagt cactcgtgct ttttatgatt
      481 acttagcatt tcctttgtct cgtgaaatac tattagaaaa tgtccagctt tcggaaaaaa
      541 tcgcccacgg taatcctagt ggaatcgatg cagccgctac tagcagcttg cagccgattt
      601 attttacaaa agggcatcct ttcgactact tttctttgaa catcgatgct tttttgattg
      661 tcgctgatac aggaatcaaa ggacaaacaa gagaagccgt caaagatgtc gctcacctct
      721 ttgaaaccca gcctcatgaa actggacaaa tgatccaaaa attaggatac ttgacgaagc
      781 aagcaaaaca agcaatcatt gaaaattcac cagaaacgtt ggcacagaca atggatgaat
      841 cacaatcact tctggaaaag ctgacaatca gcaatgattt tcttaatcta ttgatccaaa
      901 cagcaaaaga taccggggca ttgggtgcca aattaactgg cggtggacgc ggtggctgta
      961 tgattgcatt agcacaaaca aaaacaaaag cccaagaaat cagtcaagca cttgaagatg
     1021 cgggtgctgc tgaaacttgg atacaaggat taggagtaca tacctatgtt taaaggcaaa
     1081 gcacgcgcat ataccaacat tgctctaatc aaatattggg gtaagaaaaa tgaagaactc
     1141 atccttccaa tgaacaacag cctttcgtta acattggatg cattttatac agagacagaa
     1201 gtcatctttt ctgatagcta tatggtagat gaattttatc tagatggcac cttgcaagac
     1261 gaaaaagcaa caaaaaaagt cagtcagttt cttgaccttt ttcgtaaaga agccggcctc
     1321 tcactaaaag cttcagtaat cagtcagaat ttcgttccta ccgcagctgg attagcctcc
     1381 tctgccagcg ggctagctgc tttagcaggc gcttgcaata ctgctcttaa gcttggatta
     1441 gatgatctct ctctttcaag atttgctcga cgcgggtctg gttcagcttg ccgaagtatt
     1501 ttcggtggtt tcgtcgaatg ggaaaaaggc catgacgact taagttctta cgctaagcca
     1561 gtcccttccg attctttcga agacgattta gcaatggttt tcgttttgat caacgaccag
     1621 aaaaaagaag tgtccagcag aaatgggatg cgtcggacag tcgaaacatc cagtttttat
     1681 caaggctggt tagattccgt tgaaggggat ctatatcaat tgaaacaagc aatcaaaaca
     1741 aaagatttcc aacttctcgg agaaacgatg gaaagaaacg gactaaaaat gcatggaacg
     1801 actttagctg cccagcctcc attcacttac tggtctcctg acagcttaaa agcgatggat
     1861 gcggtgcgac aattgagaaa gcaaggcatt ccatgctact ttacgatgga tgctggccct
     1921 aatgtcaaag ttttagtgga aaacagccat ttatctgagg tacaagaaac atttaccaaa
     1981 ttatttagca aagaacaagt aattactgca catgctggtc caggaattgc gattattgaa
     2041 taaagggaga tttttatgat tgaagtatct gcaccaggca aactctatat tgccggagaa
     2101 tatgcagttg ttgaaacagg ccatccagca gttatcgctg cagtcgatca attcgtgaca
     2161 gtcactgtag aatctgcacg aaaagtcgga agtatccaat ctgcacaata tagtgggatg
     2221 cctgtacgtt ggacaagacg caacggagaa ttagttttag atattcggga aaatcctttt
     2281 cattatatcc ttgctgctat tcgcttgact gaaaagtatg cacaagaaaa aaacattctt
     2341 ttatcctttt atgatctgaa agtaacaagt gagttagaca gttcaaatgg ccggaaatat
     2401 ggattgggtt ctagcggtgc cgtgaccgtt gcaacggtca aagccttgaa tgttttttac
     2461 gcgttgaatt tatctcagtt ggagattttc aagattgcag cactagccaa tttagcagtt
     2521 caagataatg gttcctgcgg cgacatcgct gctagctgtt atggtggctg gatcgctttc
     2581 tcaaccttcg accatccttg gctccaagaa caagaaactc agcattctat cagtgagtta
     2641 cttgccctgg attggccagg tctatccatt gagccattga ttgctcctga agatttacgt
     2701 ttattgattg gttggacggg tagccctgcc tctacttctg atttggtcga tcaagttcac
     2761 cgttcgagag aagataaaat ggtggcttat cagcttttct taaaaaatag tacagaatgt
     2821 gtcaacgaaa tgatcaaagg gtttaaagaa aataatgtaa cgttgatcca acagatgatt
     2881 cgaaaaaacc gacaattact gcatgattta tctgcaatca ctggggtcgt catcgaaacg
     2941 cctgctttga acaaattgtg taatttagct gaacagtatg aaggagccgc aaaatcttct
     3001 ggtgcaggtg ggggcgattg cggaatcgta attgttgacc agaaatctgg cattcttcct
     3061 ttaatgagtg catgggaaaa agcagaaatc actccactgc cgttacatgt ctatagcgat
     3121 caaagaaagg aaaaccgatg aatcgaaaag acgaacatgt ttcactagct aaagcctttc
     3181 atgataaaca aaaaaacgaa tttgacttcg tacggatcat ccataaccct ttgccgcaaa
     3241 tagcagtttc tgatgtcgat ctaagtacac aggcagttgg gtttacacta agcagcccat
     3301 tttatatcaa tgcgatgact ggcggaagcg aaaaaacaaa aaaaatcaac caagatcttg
     3361 caatcgtcgc aagagaagca gatttgatga ttgccactgg ttcagtcagc gctgccttga
     3421 aagtcccttc tttagccgat acttatacga ttatgcgaca agaatatcct cacgggaaaa
     3481 tcattgccaa catcggtgca gggacttctg tcgaaagagc caaagaagct atccgattgt
     3541 ttcacgcaga tgccttacag attcatttga atgcacctca agagttggtt atgcctgagg
     3601 gagaccgtga ttttaccaat tggaaagtac ttattcaaga aactcaaaca gccattgacg
     3661 tccccctcat cgtaaaagaa gtcggttttg gcatgacaag agaaacgctc aatgatctag
     3721 ctgctttagg agttcataca gttgatatca gcggtcgaag cggaacaagc ttcacccaga
     3781 tcgaaaacgc ccgtcgctcc aagcgggaac tgagttattt agctgactgg gggcaatcga
     3841 cagtctcttc attgcttgaa gcaaatgaag cagacacttc tatggaaatc ctagcctccg
     3901 gaggtatccg caatgcctat gacatattta aagccctttg tctcggcgca aatgccgtgg
     3961 gtacttcagg aaccgtactg acgcatctga tgaatcacgg cgtagaagaa actattatac
     4021 tgatgaagca atggcaagaa gagcttcgtc tgctttatac tatggtcggc gcaacgaata
     4081 ccgcggccct acaccaacaa tctctgatct tttccggacc agtgaaggat tggtgcgaag
     4141 caagagggat cgacttagtg aagtatggaa gacgcacaga aaagagcgtg ggacaaaagt
     4201 aaagaacact tttgtctcac gctctaaaca cgtacaaacg gcggaagtaa aagcaactcc
     4261 ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa ttccttctta
     4321 tttcttggag ttaaatgctt ctgtcccagc ctcttttttc gaataaatga tgtttaatca
     4381 gctgtccttc ggtattcgtt caatccgctg atttcacaac ctctccacta aacagtgtac
     4441 taaagctctt ttaaggttgt tttccgatat aggcaagtat gcctccgtcc acatacaaga
     4501 catggccgtt aacgaaatca gatgcgctag aagccaagaa gacagatggt ccagccaaat
     4561 cgattggctc gccccagcgt gctgctggcg tacgtccaac gataaattga tcgaatggat
     4621 gacgttcacc gttttcttgt gtttcgcgta atggagcagt ttgcggtgta gcaatataac
     4681 ctggtccaat tccattacat tggatattgt attgaccgta ttcagaagcg atgtttttcg
     4741 tcagcatttt taatccgcct tttgccgcag cataagcact gaccgtttcg cgtcctaatt
     4801 cactcatcat ggagcagata ttgatgattt taccgccgcc tttttcgatc atatccggaa
     4861 taactgcttt tgaaacgatg aatggggcat ttaaatccac gtcgattact tggcggaagt
     4921 ctgccgcatc catttcgatc atcggtgtcc gtttgatgat ccctgcattg ttgactagaa
     4981 tatcaactga acctacatct tctttgatct gttgaaccat cgcttgaacc gcttcttcat
     5041 cggtcacatc gcagacataa ccttttgcat cgatgcctgc ttctttataa tgcgctatac
     5101 cttcatcaac tgattcttga ttcaagttat taaatacgat ttttgctcct gctgtagcta
     5161 aagagcgtgc aatctcaaat ccaataccat agacagcacc ggtgactaat gctacttttc
     5221 catctaaacg aaacatatcc atactaaatt ccatcatttt ctctccctta tgcttatttt
     5281 aattgatcca tttgcaccat atccatgtct gtataggtga tattttctcc gcacattgcc
     5341 cagatgaatg agtaattgct tgtacctaca cctgaatgga tcgaccagct tggcgaaata
     5401 atggcttgtt cattgtctac aactaaatgt tttgtttcat ctggtttgcc catcatgtgg
     5461 aacacacgtg tatcttcatt ggcataatca aagtagacat aagcttccat acgacgttca
     5521 tgtgtatgac aaggcatcgt attccaagcg cttcctggtt ctaaaatcgt ataacccatt
     5581 tgtaattgac agctttcgca tacatttgga tggatgtatt ggtagatttt acgttcattc
     5641 agtgtcaggc cttcaccagt ctccattggt tttacttggt cgatactgat ttttacattt
     5701 ggatatttat gatgagctgg aacagaacta atatagaatt tagctgggtt ttcagcatct
     5761 tgagaagaaa atacaacatg tcgtgtttct tttccgatat agtatccgtc ttgttttttc
     5821 atttcttctt tttgcccatc gatctcgatg aatcctggtc caccgatatt gatcacacct
     5881 agttcacgac gctctaagaa gtaatcaaca cctagttctt tgtctaatgt gatttctaat
     5941 tctttttcag taggtgtcac tccaccaaag atcattcgat cgttgtgcgt ataagtcaaa
     6001 ctgattttcc ctggttcaaa taccttttct actaaaaatt cttttctcaa ttgttccgtt
     6061 gaataatgac ggatatcttc tggactgtgt gtataacgtg tttccatttc ttgcattttc
     6121 ttctactcct tctatcgaat gatttttcca gagccgcatg ctaaaaattg tgcgacttct
     6181 tcttgactaa attggttgca gtccccatgg atcgtatgct tcaaggcaga agctgccgta
     6241 gcaaaatcaa tgatttcttg cggagacata ttggataaaa tcccatggag cacgccacct
     6301 gcaaaagcat ctccgccacc tactcgatcc acgattgggg tgattctatg gacttgggat
     6361 tccacatatt catggttcat ccacaatgtc cccttcaatt cattttcact tgctgaatga
     6421 acggtacgct tggtcgaata aaacacctga atattcggga acttttcctg cattttttga
     6481 taataataaa ttagaggttg ttcttctgct tctggcgctt tctcgatacc taacaaataa
     6541 agagcatcca tttttccagc tgagcagata tctactaaag gtaaaagctg atggattact
     6601 tcgctggctt cttgctgtgt ccataatttt ccacgatagt tgatatcgaa gctgatcagg
     6661 cagccagctt tttttgcagc ttctatcacc tgtttcgtca ttttttgcca ttttttagac
     6721 aaagctgggg tgattcctga aacatgaaat atatctattc cttggaataa ctcctctagc
     6781 gaccattcca aatgatccat ttcagcaaaa cttgatccgg cacgatcata gatcactgag
     6841 gcggcgcgtt cgccactacc agtttccata taataagtac ctagtcttga tccaccagat
     6901 aatacctgtt tcgtatctac tccataacat tgcaagtgct ttttgactgc ttttcccaat
     6961 gcgttatcag gtacttttgt agcaaaaatc gcttcatgtc cgtaattagc taaagaaatt
     7021 gctacgtttg cttcccctcc gccataatgg gcagcgaatc gttcacttga atcgatacgg
     7081 gttccctgct ctgtggacaa tcgcaacatg atttctccga gtgtcactac ttttcccata
     7141 agcagctacc tcttgatttc tttcattttt gctgcatact gttgcgcgac ttccgttact
     7201 ttgtcaaaat ctcctgtagc tgcgggtgct aaaaggttcc cacctactcc gactgttacg
     7261 acaccagcat cgaaccactc tttcatattg tccagactca caccgccagt tggcatgata
     7321 ttgacttgag gcatcggcgc cttgaaggca gaaatgatac ttggtccata agcgcttcca
     7381 ggaaatagtt tgacgatatc taccccgctt ttcagcgctg ttttgatctc ggtaatagtc
     7441 atacatcctg gtaagtaagg aatttgatac agattgcaca tctctgccgt ttcttgatca
     7501 aaggaagggc taacaatata ctctgcgcca gctaagatag ccaagcgagc tgtgacagcg
     7561 tctaatactg tgcctgcgcc aatcactatt tctggttggt ctttgtagaa tgacaaaagt
     7621 tctttgatta cttgatctgc ttgagggact gtaaaagtca actcaatacc agttagacca
     7681 cctttgatga ttgcgtggca agcattcaaa gcttcttctt tgctttttcc gcgtaccacc
     7741 gcaatcactc cggcgttttt caatcgagat aaaatatcaa tttttttcat cttctccacc
     7801 tatttcctat ttagaaattt tattttataa cttggaaatc tatacccata ttatacggca
     7861 gaaaagaaaa attgcaaacg cttttttcat tatttttgaa ttctttgctc gatttcgctt
     7921 tttgtttgct tgagggcact gattatttgt tcttttgtct catctgttaa acgatatttc
     7981 ggcatactta cactaaatgc accaatgatt tgttcgtttt tcacgattga ggctcctata
     8041 caaaaaatat cacgttcgat ttcttcatcg tcaaatgcga tgtgggtttc ttttaccgtt
     8101 tggatttcct ttttcaaccg taatgcattg gtgatcgtat attctgtgta agggatcaac
     8161 ggatgctttt caagataatc agtgtactgc tcttcactga attctgctaa tacggctttg
     8221 cccatcgcag aattgtataa tggtcgggtg attccgattt ttgaagacat gcggatcgtt
     8281 tgatgttttg gatctagttt gttcacgtac aagatctcat tgttgtccaa tacccctaag
     8341 tggatagtct catcgatctt ttgctgcagc tgttctaaaa acggtaaagt caactctacc
     8401 aaatcgattt gttcaatgtt tttattcgcg tagcgaataa gctttgcacc taaacgatag
     8461 tttttctctg aatttttatt tacgtagccg atcatcaaca aagtatctag tattttcaag
     8521 actgtagaag aggtcattcc cacacccttt gcgatcgtct gcaaagagac atcttggttt
     8581 tccgctaaat agtctagtat tttagaggct ttgatcaata cagttccata aggcttttta
     8641 ttttccatat aatattctcc tatttcttta taagaaaaat tatagcggaa ataccatcaa
     8701 tttaaaagag atatccagat ataaactacc atttctcttt acaggcaatt taatgttgaa
     8761 gatccgcttc gtttttacat caaactttga tataaagcaa tgatttcttc tactgtcgtt
     8821 tctcttggat ttcctggtgt gcatacatct cgcaaagcat cttctgcaat tgctggaata
     8881 tctgcttctt ttacacctaa ttcagaaatg gtttctggga ttccaaccgc tttgctcaac
     8941 cgatcgatct caccacaagc cgcttctctt acatcttcta aagacatttc agctgctcct
     9001 ttaatcccta gaacaagagc gatctcacgg tacttttctc cagtatattc tttgttatat
     9061 ttcatgacag ttggcaaaag tgctgcacac gctacgccat gaggaatgtt gtaccatgca
     9121 cttaacgggt gagccatccc gtgtaccagt cctaaaccga catttgagaa gcccattccc
     9181 gcaatatact gtcctaatgc cattttttct cgcccatttt gatcgccatc tacagaagct
     9241 tccaaagaat ggccgatgat ttcgattgct tttaaatgca gcatatcagt catttcccaa
     9301 gcgccttttg tgataaaccc ttcaatagca tgcgtcatag catccatacc tgttgatgca
     9361 gccaattttt tcggcattcc catcatcata tcgctgtcga tgaacgcgac gattgggata
     9421 tcatgaggat caacacaaac aaattttcgg tgatttactt catctgtgat gacgtagttg
     9481 atcgttactt ctgctgctgt tccagaagtc gttggtatag ctaatatagg taaacatgga
     9541 tttttcgtat cagcaacacc ttctaaacta atgacgtctg aaaactctgg attcgtcaaa
     9601 ataatcccaa tggcttttgc cgtatcgatt ggcgaacctc caccgattgc gatcaaacag
     9661 tctgcctctg cctctttaac aaaagccact ccatctttta cattttggat cgttggatta
     9721 ggtacgatcc cgtcgtataa agcataagcg attgctgctt tatctagaag ttctgttact
     9781 tttgttgcta taccgtgctc gatcaatcct ttgtcagtaa tgacagctgc tttgccgaat
     9841 cctcgctgct gaaattcagg gacgatcgac tcaatcgctc cttttccaaa atacgatgtt
     9901 tcatttaaaa tcattcgatt actcattttc t
//
LOCUS       DAJSMI010000093         9915 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJSMI010000093 DAJSMI010000000
VERSION     DAJSMI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.094"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            2..358
                     /locus_tag="ON430_001858"
     CDS             2..358
                     /locus_tag="ON430_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HCR3219772.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            374..724
                     /locus_tag="ON430_001859"
     CDS             374..724
                     /locus_tag="ON430_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HCR3219773.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            753..1532
                     /locus_tag="ON430_001860"
     CDS             753..1532
                     /locus_tag="ON430_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HCR3219774.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1543..2220
                     /locus_tag="ON430_001861"
     CDS             1543..2220
                     /locus_tag="ON430_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HCR3219775.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AILPAVN"
     gene            2239..3345
                     /locus_tag="ON430_001862"
     CDS             2239..3345
                     /locus_tag="ON430_001862"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HCR3219776.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3335..4426
                     /locus_tag="ON430_001863"
     CDS             3335..4426
                     /locus_tag="ON430_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HCR3219777.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4439..5179
                     /locus_tag="ON430_001864"
     CDS             4439..5179
                     /locus_tag="ON430_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HCR3219778.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5467..7407
                     /locus_tag="ON430_001865"
     CDS             5467..7407
                     /locus_tag="ON430_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCR3219779.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7494..9416
                     /locus_tag="ON430_001866"
     CDS             7494..9416
                     /locus_tag="ON430_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCR3219780.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9696..>9915)
                     /locus_tag="ON430_001867"
     CDS             complement(9696..>9915)
                     /locus_tag="ON430_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR3219781.1"
                     /translation="DYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGSE
                     EEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3233 a         1643 c         2206 g         2833 t
ORIGIN      
        1 catgattaaa gtagttattg cagaccgtat gggtaaagga caaaatgtgg ccaaaggggt
       61 agaagcagct ggtggaaaag cagttgtcgt accaggaatg ggggcagata tgcgtttggg
      121 cgatgtgatg caacaagagc atgcagatat gggtatctct ttttgtggta gtggtggtgc
      181 aggtgcatta acagcagcaa caaaatatgg ctatccagaa cgacatggta tgcgttcgat
      241 tgaagaaggg atcacagcta tcaaggatgg caaaactgtt ctaggtttcg gctttatgga
      301 tcaagaggaa ttagggaaac gtctgacaga agaatttatc aaaaagcatg gaaaatagaa
      361 agagggacat gaaatgagcg ttcaaaagac aattcagcaa aaagttatcg tctccggtaa
      421 aggggaaaca aagcaacatg cttttgcatc agcgttagca gaaattcaaa acaaaataat
      481 ggaacaatca aacgtattat tgcgaatcga accagtcaat gtggatattt taaaggcggt
      541 cgaaaacaaa tacacagaac gctttctatt tttcttcttc ccacggcaac gtattagcta
      601 tgaagtcgaa ctctctgtta ctgtggctgt aacagaaata aaattggcag acatatcgtt
      661 cagccagcaa atgactgaag atccaaatgg gattgccatt ccatttgtaa ctaaaaaaat
      721 ctaattttga atcataaggg ggaaagaata acgtggattt cgtaatcatt ctagtcaaat
      781 cggtcattat cgggggatta cttggttttg cagcatctgt cggggcagct cgaatgtttc
      841 atgcacctaa tacacaaggt ttaggagctt ttagaacatt aggcgaaatg aatgcctgca
      901 tgggtgatcc agcttctcat ttttcatttg gtttaggctt tttctttaat gcatgggctt
      961 cttcagttgg tgctggtgca ttcacacaag atgtgacgca tcgaatcatt cccaattggg
     1021 cagcggcagc acttctagca aaaaacaaag atgtcaccca gaccatgcat gatccgaaaa
     1081 aaatggggat cattggagcg gttatcgggg cagtggtggt agccttctta aacacaacag
     1141 ctgcagcaat tccagaggcg ttacaagtga cggcggtttc cgttttggta ccagcagcga
     1201 cgattctgat caatacagta atgccagtta ttttttggtt ggcagcttta gacgcaggga
     1261 aacgaactgg tttttggggc actctttttg gtggtatcgc gcaattgatt atgggaaatg
     1321 cggtacctgg tgttgttcta ggaatcttga ttggtaaagg cgtcgatgag agcggttgga
     1381 atcgcgtcac gaagagcatg atgatcgcta ttgttttatt atttattttc agtggatttt
     1441 tccgtggatt tgatatggaa atgatcaaga gcttccagct tcaagtgcct aattggcttg
     1501 ataacttgca caacatcttt agtgtgaaat aggaggagaa tcatggagac aacagaagaa
     1561 caagcaaaag tattagaagc aaagcggcaa aagagctttt ggtttgcaga ctggtctttt
     1621 ccgctaatcg taggaattat gtccgctgct gtatttgcag gaacacacat gtatgtgatg
     1681 tatggtgtgg gtgcattcaa tgaagtttcg atcgtggcga tgttaaaagc cgggctggat
     1741 ggcggatcct atggcgcagc ggcagcgttt ggtgctagtt tcttatttgc cagaatttta
     1801 gaaggctctt tagttgggat cttggactta ggcggttcga tcctaactgg gatcggaatt
     1861 ggggtaccag caattctctt aagtatgaaa atagcggcac caatcgataa ttttgcactc
     1921 tctttattga ctggtttggt tctaggtttg attgttggtg gcatcattgt cttgattcgt
     1981 aaatttacga tcaatcaatc aaattccact tttggtgctg acgtcatgat gggggcagga
     2041 aattcatctg gacgtttttt gggtccatta attatattaa gcgcgtgtgc agcatctatt
     2101 ccaatcggca ttggcgctac attaggagca ctaggattct atgcttggaa aaaaccaatt
     2161 gctggaggtg ctattttagg agcaatgatt ttaggagcaa tcttaccggc agtaaattaa
     2221 tagaaagagg gtcattaaat gtttgatact ttgattaaaa atggaaggtt agttgatgga
     2281 tctaaaatag aggtcgctat agaaaaaggc atgattaaag cagttgcagc agaaataaat
     2341 cagccggcgc aagaagtagt tgacttggag ggaaagtatt atttatccgc tgggtggatc
     2401 gatgatcatg tccactgcta tgaaaagatg aatctttatt atgactatcc tgatcagata
     2461 ggggtcgaaa aaggtgtgac gacggtcatt gatgctggaa ctacgggagc agaaaacatc
     2521 cgtgaatttt atcaattgac gaaatcggtc aagacaaatg tgtatgcgct aatgaatatc
     2581 tctaaatggg gcattgtcaa acaagatgaa cttgctgatc taacaaagat tcaagaagat
     2641 ctagtaagta ctgcgttagc agaactgccg gaatttattg tcgggattaa agcgagaatg
     2701 agtaagacag ttgttggtga aaatggcatt aagccattag aattagctaa aaaaatccag
     2761 acagaaaaca atgatctgcc attgatggtt catatcggtt ctgcacctcc tgagttagaa
     2821 gaagtattgt caagaatgga aagaggcgat gtattgaccc attgttttaa tggaaaacca
     2881 aacggtattt tagatccaaa gacagatcaa attaaaaact ttgtctggga tgcttacaat
     2941 aatggtgtcg tctttgatat tggacatggg acggatagct ttaatttcca tgtggcagaa
     3001 accgcactaa aagcaggaat gaaagctacc tctattagta cggatatcta catccgcaat
     3061 cgtacaaatg ggccagtttt tgatctagca actactttag aaaaattgag ggtagtcggc
     3121 tattcttggg aagaaatcat tgaaaaagtt acagcagttc ctgcacggaa ttttcatttg
     3181 aagaataagg gccagttagc tgtcaattat gatgcagatt tgacgatatt tgagattgtt
     3241 gaaaatgaaa aggaattgaa agattccaac ggatttacac gtatagctaa agaacaaatt
     3301 ttaccgatta aaacaattat tggaggcgtc atatatgaca attagctatg gaaagtttga
     3361 tttaaaagaa gtcattaatg catcgggaaa aatgacgatc ctaggggttt ctaaggtatc
     3421 tgaaagtgtc ctagcagcac aacgatttgg aggcgagcat ttttttgaga tgagtgatct
     3481 tgccatcaaa accggcgctt atctttctca attactgaaa gtggaagatg cgcaaatcgt
     3541 ttcttctgct tcagcaggga ttgctcaatc tgttgctgct ttaatcggac agggcgacat
     3601 gtaccatgtc tatcatccct atacagaaaa aatcgctaga cgggaaatca tcatacctaa
     3661 gggacataat gtagattatg ggacaccggt tgaagtaatg gttgctcaag gtggaggaaa
     3721 agtcatagaa gctggctatg caaatatgtg tacaccggaa catgtttcca tgatgattac
     3781 ggaaaaaact gctgcgcttc tatatattaa aagtcatcat gcagttcaga aaagtatgct
     3841 gacggtggca gaaatggtag aagttgccaa agctcatcgt ttgccattga tcgttgatgc
     3901 agctgcggaa gaagatttat tcaaatatag cgaaatgggc gttgacttag tgatttactc
     3961 tggggcaaaa gcaatcgaag gtccgagtgc cggactggtc ataggaaaaa agaagtatat
     4021 tcaatggatt cggctacagt ctaaaggtat tggccgagca atgaaaatcg gtaaagataa
     4081 tattttaggc tttacccagg cactagaaga ttatttaaaa aatgggagcg aatccggtga
     4141 ctccatgaag caacgattag cgccttttgt taacgcaatc aatcaacttc ctgacctttc
     4201 agcgaaaata gttcaagatg gtgccggcag agatatttat cgagcaagtg taacgattaa
     4261 tggcgataaa agtgcgaaag aagtcattca ggaattaaaa gcacgaaatc cagcagtcta
     4321 cacccgagaa tatcaagcaa ataacggaat cattgagttc gacattcgtt ccgtcagcca
     4381 aaacgaaatg gaaaaaatta taaaaagatt gagagaaatc atgtaggagg cttttaaatt
     4441 gtcagttatc ccaaattatt ataaaaatcg tgtatgttta aatgtattag ctcattcagt
     4501 tgaaaatgca aaagcatgct atgaagcagc ggaagggcat gtcgtactag gtgtgctatc
     4561 gaaaaattat gctagcgatg aagcagcaat cgaagatatg aaaaggtacc aagacgcaac
     4621 caataatgcg ttgtcagttg gattaggcgc tggagatcca aaccaaagtc agatggtttc
     4681 gcgtttatct ggggttttac aaccacaaca tgtcaatcaa gtatttactg gcgtggggac
     4741 ttctcgtgct ttattgggcc aaaatgatac agtcatcaat ggattggttt caccaacagg
     4801 taagatcggc tatgtcaata ttgctacagg tccgttaagt tcacaagcgc cagcagctga
     4861 agtgcctatt gaaactgcca ttcaaatgct gcaagacatg ggaggtactt caatcaagta
     4921 tttcccgatg aaaggcttag cacataggga agaatttaaa gcagttgcgg aagcttgcgc
     4981 aaaatatgaa ttctatttag agccaacagg aggaattgat ctttcaaact ttgaagaaat
     5041 tctgaaaatt gcaatcgatg caggtgtaaa aaaagtgatt cctcatgttt atagttcaat
     5101 tatagataaa gaaacaggag atacgcgtat aggagacgta aagatactat taagcatggt
     5161 aaaaaaaatt attcactaac tcattagaaa ataagaagct tattccagaa tgaaattaaa
     5221 tggaataagc tttttataat taattacgga aattgttaat gtagataata atttagatgt
     5281 acgtaagcag aagtacagaa atgtaagatt ttccaatatt attttattca acataataaa
     5341 tcgtatatca atttttttag cgaaatttct atttattcca agaatttcag ttattacgga
     5401 atcattttgt gcttaataaa aagaaaccgt ttttatatac tataaatgta gaaaggcggt
     5461 aggaaaatga aaaaaattat tgcaatcaca tcctgtccag ttggtattgc ccatacatac
     5521 atggcagcag aaaacttaga gaaagcgggg aaagcagttg gcgccgaggt aaaagtcgaa
     5581 acacatggtt ccatcggtat tgaaaatgaa ttgactgcaa gagatatcga agaagcagct
     5641 ggtgtaatca tcgcagcaga tactaaaata gataaatcac gattcggcgg taaacctttg
     5701 ataaccgtag gtgtgcaaga aggaatccac catgcagatg agttggtgag agatatttta
     5761 gcaggaaaag cgccagtcta taaaagtacg gaggcaatta tttcttccga aaataaaagt
     5821 gaatcagaaa atttaggtaa aaaaatctat aagagtttga tgaatggcgt ttcgtacatg
     5881 gtacctttcg tagttacagg agggttattg atcgcgattt ctttaacatt aggaggaaca
     5941 gctacacctg aagggatcaa gataccagaa ggaacgattt gggcaacgat gaatagtatc
     6001 ggaagtatcg ctatggggtt gatggttcca attctatctg catttattgc gcaaagtatt
     6061 gcagatcgtc caggacttgt tcctggattt gttggtggga tgttagcggc aaacggagca
     6121 ttatatggta gtgatgcgaa tgcgggattc ttaggcggga tcatcactgg atttttagca
     6181 ggattcattg ctttaggaat caagaaagtg cgagtaccga aagctttaca atcgatcatg
     6241 ccaattattg ttatcccaat tttaggatca ttggcagttg gttttgtgtt tatctatgtt
     6301 attggagaac cagtagctct tctatttagt tcattgactc actttttagc agggatgcaa
     6361 ggaggaagtg aaattgttct agctatgatc ttaggagcga tgattgcttt tgatatgggg
     6421 gggccgttca ataaagtagc ctttttgttc ggatctggct tgattgcaga aggcaattat
     6481 tccattatgg ggccaattgc tgtagctatc tgtatccctc cattagcatt ggggctggca
     6541 tcattgattt taaaaaccaa atttacgaaa acagaacgag aagctggtaa ggcgtcctta
     6601 gcaatgggac tgtttgggat tacagaaggc gctattccgt ttgcttcaac agatccacta
     6661 cgggtgattc caagcattgt aatcggggca atgacaggaa gtgtgatcgc gatgctttca
     6721 ggagtgacag atcatgtagc tcatggcggt ccgatcgtag cggttttagg agcagtggat
     6781 cacgtagcca tgttttttgt agcagtgatt gctggtattg ctgtaaccgt gctaatgtta
     6841 agaatcctta aaccgacatt acaacaaact gcggttgctg cagcaaatgg tgtgacagta
     6901 gcagatctat cagagactga aacaagtgat gtttatacga aaactgaaac ttttgattct
     6961 atcacggata ttatgtcaga tgaagcaatc gttttaggtg aaacagcaac aacaaaagac
     7021 ggagtaatcg aagatttagt ccaacgttta gaaaaaacag cggcgatcac agataaaacc
     7081 agatttaaag aagtcatcta tgatagagaa catgaaagta caacgggaat cggtatgggg
     7141 attgctattc cacatggaaa atcagcttat gttcttaaac caacagtggc atttgccaga
     7201 agtcaatctg gggtagattg gcattcttta gatggaaaac ctgcacatat gatctttatg
     7261 attgctgtac ctgaaaacag ccaaggtgat atgcacttga aaatactgca acgtctatca
     7321 cgtaaattga tggatgatga cttccgtcaa gcactgatgg aagcgccaga taaaacaaca
     7381 gttcaccaat tattaagcga aatgtaacat aagatgaaaa atcgaccgtg atgagacata
     7441 atagatgtgt tatcatgatc gattttttat ttagaaggga gtttctgcac attatgaatc
     7501 agcgtcagca atctttgatc tatcaattaa gtgagaacaa taattatata acaacaaaaa
     7561 agctagcaga gcatttccat gtgtcagaaa gaacgattca tagcgatttg aataccattg
     7621 aaacttggtt gagagacaaa catctgcctt taatgtttga aagaaaaaaa ggtacaggta
     7681 tcttgttgaa tggtctgcct cacgaaaggg aacaactgaa acgagcgctt aatgaagaga
     7741 ctcaaacaga aatggataaa aaacagcttc gtcagttatt gattttccat ctgctagtag
     7801 caaaagacgg atttagtctt gaagaacttt cagaaaaact atatgttgga aaacgtacga
     7861 ttcgtaaaga attgacagag ttgcaaagct tttttgagta tcaccattta cagctagtat
     7921 ccaaaacaaa actaggaact tttcttaaag gcgatgaaca agaaaaaaga cagttgcttg
     7981 ttaagacatt acgaaatatg cagaaagaag atccacaaag tccgtcgttg aaagaattct
     8041 tcgctaaaga tacattaaaa gttatccaac atacattaca agatgttttt tatgaaaacc
     8101 atttagaaca gccgaatgga ctggctagtg tggagattca catctacttt atgctcgagc
     8161 gaatgaaaca gtaccagaaa gtgaaattaa gtaaagacga aaacgaagtc gtggaacaca
     8221 cccaagcaca aaagttaagt agtcaaatat tagcaaaact agccaccatc tatccaattg
     8281 aattttctcc tgatgaaatc aattatctgg ctttgcgtat cgccaatctt tttacaaatt
     8341 cacaagcagc cacacgtttt caaaaggaat ctagcgcact tgctgatcat ttgatcgaac
     8401 aagtggaaca gtttctaggc tattcattaa aagaagatca gctactaaaa caaaatttaa
     8461 ggtctcattt atcttcaact tattttcgac tccattatgg tttgcagatt tctaatccat
     8521 tgacgaaaaa tgtgtttagt acgtacacac aacttttctt agttttacag ttaattttag
     8581 aagattattt tgaaaaggaa aatttttatg ttccacaaga ggagacagct tatttaacga
     8641 tccatttcca agcagcgatc gagcgacaca aacatcagaa aagtagaaga tatcaaacgg
     8701 ttttagttag tgaatattca aaagctatgg ctacttttct cgaagcacgg ctgaacagag
     8761 aacttccaga attaacgatt atggatctag ttgaatataa gccagaatta aagagacaag
     8821 attttccctc tgttgatttt atcttggcta cgttaccatt taaacatata agcattccag
     8881 taatcgagat ctcaccaatg atcacagaaa ctgatctagc ctatttgaca aaatatatgc
     8941 tggagcatgt gcctatcaag aagaaaaaaa catttgatct agcaagcttt actcatccat
     9001 ttctgatctt tcctcaactt gaacggacag acccagccga tattttaaac tttatgggaa
     9061 acgtcttagt ggaacatcat tatgtggagc cggaattcat cgattctgtc ttggaaagag
     9121 acagtcactc cagtactaga gtagcaccat ttgtaacaat ccctcacggt aatccgctgt
     9181 atgtgaagca ttcgatgatt tctattgcta caatgaaaga accagtccta tggcacgggg
     9241 aacagatacg tatcgtactg ctattagcaa taaagaaaaa tgatttgaaa catgcagagt
     9301 tcaaaaaaat attttctgtt attcactatc tagaaaagca gccagaacaa atggctcagt
     9361 tgatgcaatc caatcatccg ctagaaatct taacgttgtt atctagttat gaataagtag
     9421 cgattaaaaa actgccttta tatttataat aattctatct atttatttgg aagtagaaaa
     9481 ctaaatacaa aaaaagagga tgtggccaaa gttgtggcac atccttttat tttgagtttc
     9541 ttataatttt ttatagttgc ttacgatgtc ttgactactg gtaaacggtg ttcaagagac
     9601 aatttgtcgg caataaggca caattttgta aaatatggaa agctattttt ttaacattgt
     9661 ttcttagtgc ttgaaaaatc attttgaata cttctttata cgatcgatgt acgttgattt
     9721 aagtctaaaa cttttctttt gattccttct ctgacttttc tgaattccgt taatatttct
     9781 tcttcgctcc cgacagcttg tgcaggatct tttaggtccc agtgaaggtg ttcgtgattg
     9841 tttggaacgg cagggcactt gtctttggca tcaccacaaa gtgtaatgat caagtctgcc
     9901 tgagtaaagt aatct
//
LOCUS       DAJSMI010000094         9762 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJSMI010000094 DAJSMI010000000
VERSION     DAJSMI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.095"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..231
                     /locus_tag="ON430_001868"
     CDS             <1..231
                     /locus_tag="ON430_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3219782.1"
                     /translation="TRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNH
                     IKVLKRNVYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            complement(448..1290)
                     /locus_tag="ON430_001869"
     CDS             complement(448..1290)
                     /locus_tag="ON430_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3219783.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(1327..2793)
                     /locus_tag="ON430_001870"
     CDS             complement(1327..2793)
                     /locus_tag="ON430_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3219784.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(2849..4693)
                     /locus_tag="ON430_001871"
     CDS             complement(2849..4693)
                     /locus_tag="ON430_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3219785.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            5295..6245
                     /locus_tag="ON430_001872"
     CDS             5295..6245
                     /locus_tag="ON430_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3219786.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(6290..6923)
                     /locus_tag="ON430_001873"
                     /pseudo
     CDS             complement(6290..6923)
                     /locus_tag="ON430_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(7183..7731)
                     /locus_tag="ON430_001874"
     CDS             complement(7183..7731)
                     /locus_tag="ON430_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219787.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(7732..8589)
                     /locus_tag="ON430_001875"
     CDS             complement(7732..8589)
                     /locus_tag="ON430_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3219788.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(8875..9162)
                     /locus_tag="ON430_001876"
     CDS             complement(8875..9162)
                     /locus_tag="ON430_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3219789.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(9152..9481)
                     /locus_tag="ON430_001877"
     CDS             complement(9152..9481)
                     /locus_tag="ON430_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3219790.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(9558..9704)
                     /locus_tag="ON430_001878"
                     /pseudo
     CDS             complement(9558..9704)
                     /locus_tag="ON430_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
BASE COUNT         2959 a         1978 c         1487 g         3338 t
ORIGIN      
        1 actagagaca ttagcggtct gccagaaacc tatacaacca ctttacgaac gtttaaaaag
       61 tttctgccac agatccaaaa tgcgcttcac tacagctatt caaacggacc attggaatgc
      121 ttaaacaacc acatcaaagt attaaaacgc aatgtgtacg gctttcgcaa cttttacaat
      181 ttcaaactac gaattatgat tcgtcatggc aaaacatttt taaccaaata aacagaacag
      241 aaagaggttt tcttctttct gttctgtcca aattattcta agatttctct ttaccaacat
      301 catttgacaa agagccgaga ttttttatta tcttacgtta atccttttta aatgtctctt
      361 atagataaca aacagctgga aatcaccttg tttttactat aatccatctc taaagatttt
      421 aaaagcatca atagtattac tgagcgctca cccattagca tgtttcatca accgattgat
      481 gtggacacct aggtatacaa cttcatcgtt cggaattgtt ttatgataaa ctttttcaat
      541 gtagtctttg atcttagctg cgctttccat ggcagacggg tataagatcc acatttgatt
      601 gtagaattct tcctcaccat catccaacaa agcatcagaa taatatcttt ctacaaagaa
      661 acgtacatgt gtaatgaagc gggcataatg gatcgaatta gtatcgatat ctttctgcaa
      721 cgaataccgg accaaattga cgatcccgcc gatcattttt gcatacttga atccagcgcc
      781 gctatcagga tcttccgatt gtgcattgat caaatggaaa gcaatgtttg aggcttcctc
      841 tttaggaaga tcaattccat attgctcgtt caaaagggtg agtgcgtact tcccaatttg
      901 aaattctttg ggatagtaga tttttatttc ccagtacaaa cggtttgcaa tatttacccc
      961 tttttgtgcg cgttcaactg caaaatgcag gtggtctgtt aaggttaaat agactccagg
     1021 attcagtttt ttatctaatc ctttttctgc ttcaaaaacg atagtacgag tgatttcaaa
     1081 aaattcaata ggtatttctt caactaactc taatagttgt gaagatttct tttcttcaaa
     1141 aggcagaaat attttatcta ctttatctcc tgatatttca tccccagctt tctgtccaaa
     1201 gcctatccct ttacctaatg caatcatttc tttgccattt tcttcaacca gtaccacact
     1261 tgaattcaat acttttttta ttgaaatcac tcattatccc cctatcgctg cttaaaaaga
     1321 tcgagactac tcgaataagg atgcaccgtt tgtttcgatc acttctttgt accagttgaa
     1381 cgaatccttt ttcaagcggt tatatgtccc atttccttca tcgtctgcat ccacataaat
     1441 aaatccatat cgtttagaca tttgagaagt tcctgcacta actaaatcaa ttggtcccca
     1501 gctagtataa gctatcaaat ctacaccttc atcgattgct tctttcattt gttggaagtg
     1561 tgctttaaag tagtcgatgc ggtacggatc atggatttta ccatctacta cctgatcgac
     1621 tgctcccata ccattttcca caatcatcaa cggtacttgg taacgatcat aaagttcgat
     1681 caacgaaatt ttcaaacctt ttggatcgat ttgccagccc caatctgttg aaggcaaatg
     1741 aggattctta acccctaaaa cagtatttcc cggtgtgcgt tctgcgtttg gatcagctga
     1801 ttctgccatg gacatgtaat agctgaaaga aacaaaatct acggtatgtt tctttaaaat
     1861 ttctcgatct tcatcttcca ttttaatctg aattccccga ttttccaaat ccttttcgat
     1921 tagagcagga tattctccct taacttgaac atctgcataa aaattatttt ctaagttctt
     1981 tttcaaggtc agctcaacgt cttctggtgc gcaagttaat ggataagtca tcaatttcgt
     2041 taacatacaa ccaacttggc ttcctggaat tttttcatga gcatcttttg tcactaacgc
     2101 actcgcaaca aattgatgat gcaatgcttg atagatatct tgagcttctt gaccttctgc
     2161 gcttttctct tggataattc cagctgttgt aaatggatga cggtggatgc tgtctatctc
     2221 attaaatgtc agccaatatt ttacgtattt gccgaagcga tcaaaacaaa cgttagcgaa
     2281 gcgaacaaaa tcatcaatta ctcgacgctc cacccagcca ttatatttaa ctgctaaagc
     2341 taatggcatt tcataatgag atagtgtcac aatcggctca attcctaaac gttgcatttc
     2401 tttaaagacg ttttcataaa atgcaacccc tttttcattc ggttccagct cttctcctgt
     2461 agggaacaag cgagtccaag caatagagag gcgcaaggtt ttaaagccca tttcggcaaa
     2521 taaagctaaa tcttctttat agtgatgata aaaatcaacg ccccttcttt ttgggtacca
     2581 tttatcagat ttgtcttcac tagcttcttt tatagactgc agggtgacat gcatatgagc
     2641 agcaaaatct ttgttgtcta aatttggttt ataagtagcc atatccgcta ctgaaagacc
     2701 tttaccgtct acattccaag caccttctac ttgatttgct gctatcgcgc cgccccataa
     2761 aaatcctttt ggaaaacttg ttgtgtttgt cattattcta ttctcctcta ttccttcatt
     2821 caatatattt tttttcacta tcttgtgttt aagctatcgg actgatagct aagagatcac
     2881 tatcattggt taccaatttt tctaggttaa atgaacggat agtgtaatta tccttattgg
     2941 ttaccacaca gataactgtg gggtcgaatc ctgcttcttt gattaaatca atatcaaatg
     3001 caatcagctt ttgtcctttg gctacttttt caccagaacg aacaaatgcc tcaaaccctt
     3061 ttccttctaa ctgaacagta tcaataccaa tatgaaaaag aagttctgca ccattagccg
     3121 tttttaaacc aatagcatgc ttcgtttcaa ataccattgt gatttctcca tcttcaggag
     3181 ctactaatat cccttcttca ggaatgattg ctactccatc acctaccata ccactagaaa
     3241 aaacagtgtc ttttacttct gatagtgaga ttacttttcc attcacggga cttgaaagaa
     3301 cagcatctga aacaacggcc gtttccatct tagtttcaac ttcattgaca acttctttat
     3361 ctttataaag aaccaacgct gcagcaaaag caaccacaaa tgaaatcccc gatactgcaa
     3421 atgcccaaac cagattcatt gggtcagctt tgtcagcgaa catcgtgata cttgcaatcc
     3481 caggacccac taaagcaaag gcttgaagtg caacgattcc tgcaaatgct cccccgatca
     3541 ggctagaaaa cataacggca tataaaacac gtttgtgcag aatcgttacc ccgtaaagag
     3601 ctggttcagt aattccgaat aatgcagata tacccgcaga aacagccgta gatttcaatt
     3661 ttttgtcttt tgttttgata ctgacagcaa agcatgcgcc agcttctgaa atattgtgtg
     3721 ccaatgaagc cgggagataa agaagttctg cacccaattc actcatggat gaaacagcgt
     3781 aagggatcat tgctttatgc atacccgtta caaccattaa tggcagtaca gctgctaaga
     3841 ctccggttgc aacccatcca aattgattgt acaaagacac aatgatagaa gagaaagctg
     3901 ttcctacatt ataacctaat ggtcctagaa gcaacaaagc aataggaact gtaatcaata
     3961 agctcatcat aggtacaaag aaaatacgaa ttggttttgg tgaaactttt gtaaaccatt
     4021 tttcaagttg agcataaaat aaaacagtta agatggctgg gaatacttga gatgcatacg
     4081 ctatattatc caatccaaaa gataagaatg tagcaccttc cgccaacata gctgtcattt
     4141 caggaaggat caatgcaccg acagcagata ctgcaaccaa ttgattgact ttcaatttat
     4201 tagccgtagt cattgccacc agaataggca agaaataaag cggtgccccg ccgaccaaat
     4261 ttaaaatctg ataggtttga gattggtcac taatcagccc taagatagaa gctagtagca
     4321 acaaggactt caatactcct ccacctgcaa tcgcaggaat taaaggttgg aaaatagaaa
     4381 taataaaatc taatagaact gaacctaatt tttgtttttg ttttggtgct tcttcagtat
     4441 ttgaaacatt gtcacctaat aacgctttca cttcatcata cacttcaaca acatcgtttc
     4501 caatgattac ttggcattgc acattttggc gaacgcccat aacgccatcc actttttcta
     4561 actggctcat atttaccttg ctgttgtctt ttaacgtaaa acgtaaacga gttgagcaat
     4621 gctccaaatg ggatatgttt tcttccccac cgatgtactg caatacttct tctgctgttt
     4681 gtttgtaatt catggtgttt ccctcttttc ttttttgagt tatgaacttt tgagtcgaag
     4741 tggccatcct caacacaaaa aacaggcaga aactaaataa attgacaaca aggcaaacgt
     4801 gtattgaaac gcatacattg ttattcaact tatttaggtc ctgcctgcat taccagtcac
     4861 atgcctgttt aagttatatt caatttaaca tattagaatg taaacgtaaa taataaaatg
     4921 attttttaat cattttttca ttttattcgt tcaatatagt tttcgtgttc taaaagtttt
     4981 gtcttttttc taagaatatc ttccaacata tataactctt attctcttct atcttctagc
     5041 agatgttctg tctttttaag acctagtata aaaagaatgg gttcattttt tctttcttgc
     5101 aggatgaggt tttttatata aactttattt gatataaaga aaaggcattc ataattgtgc
     5161 tatactacta aaaaatgata atacagtgaa ttagattagc gctttattaa attaagagga
     5221 cagcatatcg atattgcttc cttttttttg ctatttattt aagggataga agtttagaaa
     5281 ggactgttta taagatgaaa aatgatatag tgacttatgc aaacacaaaa tgtgaagaaa
     5341 ttggcatcag taatttagaa gagtgctttt ttgacacaaa tgacctgtcg caatttttaa
     5401 gaacgggaag aacaaatgcc agtaaacttg ccaatgactt agttgcaaag aacgttctat
     5461 ttaaaatcaa tactcgtcca gtgttatttg ccatcaaaga gcgctttgaa gagcactttt
     5521 tgattccggt aaaagatgta tacgaaagta tatctgaatt aaaaacagac ttggagccca
     5581 attctacaga ttccattcta caaaagatga ttgggtatga tatgagctta tccgatgcgg
     5641 tggaacaaat gaaaactgct gtcttctatc ctggaagagg gcttcctatg atgatcactg
     5701 gagatactgg agttggaaaa agtttttttg ttcatactgc ttatcagttt atgaaaaaag
     5761 tgaacgttat tcatgagaat gctcctttta aagtacttaa ttgtgcgcaa taccataata
     5821 atcctgaatt attgtcgggg atactatttg gctatgttaa aggtgctttt accggcgcag
     5881 atgaagatcg aaatggattg ttgcaagatg cagacggtgg agtactgttt ttagacgaag
     5941 tacacagatt gcctcctgaa gggcaagaga aactatttgt ttatatggat ggcggaactt
     6001 attctagagt cggtgaaact atccatcacg cctccaatgt acgtttagtt tttgcgacta
     6061 cagaaggcaa agaggatttt ctggaaacct ttatgcgaag aatccctata catatccata
     6121 tcccaaatat tgaggaacga ggtattttag aaaaaaaaca aattatcgaa aatactctct
     6181 ttagcgaaag caaaaacttt agcaaggcta ttgatgtttc tcctaaagtc actagtgttg
     6241 cataaatata gtcagaatat tctgaattat tattttcttg aaaaaagtaa aaaaaatcgt
     6301 atcccaaact ttcatctaca tttccagaaa gatcttcatg ttgataatca attcctgaat
     6361 caatgaccgc taccttcaca tccttagaac ccttggtttc ttcccaagcg gatggcatgc
     6421 taatctctct taaattagct tgtttactcc aattaggatc gttatcgatt tcctaaagaa
     6481 tcagttgtat ttggctcctc aacatcattc aacggctcta gttcaaccac atagtttggc
     6541 tctgcataat tgaccatatc agaagcatta aatgttctta ctgcctcttc cacactaatt
     6601 tcttcgttca atttcactaa taatacttga tctcgcttat tataatcgct ttcatttgtc
     6661 ctaagagtca aatctttttc caccataact tttacaccca ataaattttg cggtttattt
     6721 ttctctgttg aaattctttg tgtagccttt aaactgacaa ttagctcatt gggtacatac
     6781 tctatttgag agtcatctaa ttggctcata atcctttctt tttggttagt atctgcagtt
     6841 acttgtttag tatgagccat gaacccaaac atactcaccc caacaaccaa tcctaaatat
     6901 attttctttt taaacttttt catctacatc tcctcctaat agttttattt tagagttagc
     6961 tctattactt atttactaac tcaacaacag attatcatac acttattaat gaaatcacaa
     7021 tatctcatta aaacacaccc taaaatcata accgttccta tgctcatttt ttggattttt
     7081 taatcacgta accagaaaaa taaatcccct ctatttcacg caaatcaaag aagaactaag
     7141 taactaccgt ttaaaggtag ttacttagtt cttctttgat tattatctaa ctttattatt
     7201 cattccccac cctttgcgca gtgagtcttc ggtatcttgt tttatctgac tggtagttct
     7261 tattatttct ccacgatcat ctaattctgg aacaattgga tatttttctc tgaacaagtc
     7321 tagttctttc accacgtcat taaacaaatc ttgtttatct tcttgcgtaa gattctgttt
     7381 aatatactca tctaaagctt ttctaagcaa ttcatctctt gtcatattag tctcatactt
     7441 ttctactaaa actctggaag tcgcatttaa caaagtcgac agttttaaag gatgcttggt
     7501 ggaataaacc ttatcaaact ttctgattgg tgcgccaact ttttttgttc ttcctctact
     7561 ctgtctttca acattggagg aagtttttat ttcttgaact tggtgcactt ctaattgctt
     7621 attttcagat ggtgtaattg cttgctgctt tttctttgct tctgcttctt tttttatttt
     7681 ggcgagtcct ttgttgttcc caaaaatatc aagtcctttg atgtcagcca attactccac
     7741 ctccgcatac caattctcgt gttcttccaa ttctttcaaa atactgataa ataattcgtg
     7801 gcttttgcga tcccattgtt ttaagagacc tttctttgtc ttctccatcg taatacctga
     7861 tccgtcgtat cgttttaagc gcgcattttt gagtacaaca tttttaatca aatgttcccc
     7921 ataaatctct tgggcttgtt tatacatttc ctgatctact gcgtcaccgc tttccaacat
     7981 gaaaggaata acaccagcaa catataaatt tgtatcaaat tctttcacaa aaaagtccat
     8041 atactcaaga tattgcatcg catttctcat cgattttact tgggtctgta agacgacgat
     8101 tgcccaatcc gcaatgtaca tcgcggattt actatataca gaaatagaag gtgggacatc
     8161 taaatagatg acgtcaaact catcaaataa tggttctagc ttttctttca tatatttgaa
     8221 ttgggataat gaatcattat attggtcttt gatccaatca tcatacattt caaaggacat
     8281 gtctgctgga agtatataga ggttattgat tactttatag acagcctctt taggtggaag
     8341 attgtttatc aacacgtcag tcacagtatt ttcgtaaaca atcggttcac cttctccgcc
     8401 tccttcttcg ttagtgaagt tcatatgcgt taagttcatc acatcagaag tatttccttg
     8461 catatccaag tcaattatca aggttctttt tccttcatta actgctgatt caaaccctaa
     8521 catttgcgcg cacgtacttt tacctacgcc acctttaaaa ttaccgatta catatttctt
     8581 cgctaccacg ttcctacctc ttttctttta gcaatattca tttgatacta ttataacata
     8641 tttattttta gttactacca aaaaaaagat atatatgtac taccttataa ttaataagta
     8701 aaaggtagta cataaataag gaaaagctga aatatattga tttatagctt tcctcaaagt
     8761 aatgacgtta tttaatttat cgagaagcag agaatactcg cgactttagt catgtagacg
     8821 aatcagattt tcgataaaaa agaagccgtc cttcttaaga acgacaacaa atcactagat
     8881 taattttttc aactgttcat aaaagttttc atgcgttcca cataacaata ctggaataac
     8941 cttgttttct ataatctcgt aagctactct atatgttgtg cccgcatatt tgaacccact
     9001 agaccaaata cctgataaat ccccagtttt ttgttcaccc ttatatggat caatcgcaat
     9061 ttcatcataa attaaattca aaaatttttc tttcaatggc ttctctttta gcttttttaa
     9121 atacttaatg acatgttttg aaggaacgat ttcataagcc aatttctgtt gccaactcct
     9181 ctttagtcat tactttagaa ttagtaagtg tatcttcagc aatatctcta aaggaagaat
     9241 gcattttgtt ttttctattc ttaaattcta caagaatctc atctttatcg tagccttctg
     9301 caattatatc agacaaaata tcttcgctaa aatcataaaa ttctttttgt ggttcaacaa
     9361 attcataaag aataccattc tctaccaact ttgcttcaac ttcgactcca ttaggaacat
     9421 taaaatcttt aggtactgtc aacatgattg aattgccttg ttttcttgtt tttgtaatca
     9481 tttaaatccc tcatttctaa cagtatgtaa ttacttagta aatacattat accacgagat
     9541 aatgtattta caagtaatta ctgtgactcg taatgcgtcc ttgctgaata aatttcaacc
     9601 acgtgttcat cttcatctac cttataaaca acccggtgtt gtctattcaa tcttctaaaa
     9661 tacctacctt catgtgtagg ttttaacttc tcaaatgatt gggttctgtt gcaaagtttt
     9721 aaataaagaa taaaatccct tacggtatct atgatttaag ct
//
LOCUS       DAJSMI010000095         9714 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJSMI010000095 DAJSMI010000000
VERSION     DAJSMI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.096"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            282..521
                     /locus_tag="ON430_001879"
     CDS             282..521
                     /locus_tag="ON430_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219791.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="ON430_001880"
     CDS             complement(883..1143)
                     /locus_tag="ON430_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219792.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="ON430_001881"
     CDS             complement(1328..1825)
                     /locus_tag="ON430_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCR3219793.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="ON430_001882"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="ON430_001882"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCR3219794.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="ON430_001883"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="ON430_001883"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCR3219795.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="ON430_001884"
     CDS             complement(4547..5209)
                     /locus_tag="ON430_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3219796.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="ON430_001885"
     CDS             complement(5219..6034)
                     /locus_tag="ON430_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3219797.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="ON430_001886"
     CDS             complement(6296..6739)
                     /locus_tag="ON430_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCR3219798.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6770..6883)
                     /locus_tag="ON430_001887"
     CDS             complement(6770..6883)
                     /locus_tag="ON430_001887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3219799.1"
                     /translation="MEADNSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(6873..7211)
                     /locus_tag="ON430_001888"
     CDS             complement(6873..7211)
                     /locus_tag="ON430_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3219800.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="ON430_001889"
     CDS             complement(7199..7576)
                     /locus_tag="ON430_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3219801.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="ON430_001890"
     CDS             7800..8993
                     /locus_tag="ON430_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3219802.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="ON430_001891"
     CDS             complement(9159..9581)
                     /locus_tag="ON430_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3219803.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2896 a         1935 c         1590 g         3293 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacattct tgcatttcag caattttttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag ttattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggctact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgat
//
LOCUS       DAJSMI010000096         9710 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJSMI010000096 DAJSMI010000000
VERSION     DAJSMI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.097"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..391
                     /locus_tag="ON430_001892"
     CDS             <1..391
                     /locus_tag="ON430_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR3219804.1"
                     /translation="KNAEGKYYGGLVDTDKDGVKEAVWVDNVANAAILKSDKEGHFEI
                     TGLTEGEYSLEETKAPENYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAIPLTGSNG
                     FQTYVLISCLLLGAGGLSAVVYFKKKA"
     gene            404..1159
                     /locus_tag="ON430_001893"
     CDS             404..1159
                     /locus_tag="ON430_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3219805.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            1156..1980
                     /locus_tag="ON430_001894"
     CDS             1156..1980
                     /locus_tag="ON430_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219806.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQPVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            2282..4381
                     /locus_tag="ON430_001895"
     CDS             2282..4381
                     /locus_tag="ON430_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3219807.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVLDGKNYDGALWYVEANGTWKKADTGGKLTVSSNTVTYTASDSFLANA
                     ANYGRDYILQLHMKAKNDAKGIAPNVATVTFNNDPNKGTQRTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQDPVEA
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(4419..6476)
                     /locus_tag="ON430_001896"
     CDS             complement(4419..6476)
                     /locus_tag="ON430_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCR3219808.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNHEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(6590..6802)
                     /locus_tag="ON430_001897"
     CDS             complement(6590..6802)
                     /locus_tag="ON430_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219809.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            6989..7456
                     /locus_tag="ON430_001898"
     CDS             6989..7456
                     /locus_tag="ON430_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219810.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            7453..7695
                     /locus_tag="ON430_001899"
     CDS             7453..7695
                     /locus_tag="ON430_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCR3219811.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            7713..8015
                     /locus_tag="ON430_001900"
     CDS             7713..8015
                     /locus_tag="ON430_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219812.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            8086..>9710
                     /locus_tag="ON430_001901"
     CDS             8086..>9710
                     /locus_tag="ON430_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219813.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDT"
BASE COUNT         3467 a         1485 c         1877 g         2881 t
ORIGIN      
        1 taaaaacgca gaaggaaaat attatggtgg attagttgat acggataaag acggtgtcaa
       61 agaagcagta tgggtagaca atgtagcaaa tgcagcgatt ctaaaatcag ataaagaagg
      121 gcactttgaa atcactggtt taactgaagg tgaatattca ttggaagaaa caaaagcgcc
      181 tgaaaactat caaaaattaa cagaagatat tgcatttaaa gtaaacaaaa actcatttaa
      241 agaggaaaat cgaatcactg ttaaaaataa tcaaaaagca gctattccat taacaggtag
      301 taatggtttc caaacgtatg tattgatctc ttgcttgttg ctaggagcag ggggactttc
      361 tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta ttcgtaagcc
      421 cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt cggcagtagc
      481 agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac agcaaagaat
      541 tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga aaatagagtt
      601 aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac caatttatga
      661 cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa ctggcgatat
      721 tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt ataaagataa
      781 tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta aggtaaatga
      841 agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag aattacaaaa
      901 aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt taatgacatg
      961 cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag tttccttcag
     1021 taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt cattggattg
     1081 gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt ataaaaaaat
     1141 aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc agctctcttt
     1201 tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag taccactcaa
     1261 acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa tactacagaa
     1321 gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc agaaagttct
     1381 tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa gcaagccatt
     1441 catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca agtagcccag
     1501 caagaaggta tcaatggcgc aaagtttatt gtctacgata tcacgaatat cttagaagaa
     1561 atgtcacaag atgataatga gaaaaatcaa cctgtagaaa taattgagtc aaaattgaaa
     1621 gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg agaaaccaaa
     1681 acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc aaacaaaaaa
     1741 caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag tgaagatatt
     1801 gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt ttggttatac
     1861 cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc aactggtgta
     1921 aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt tgataattag
     1981 gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt agccgcaaca
     2041 ttattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata tcaacgattg
     2101 ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat taaaaagctc
     2161 ttgatcagtc caatttttat ggttagtttg gttcttctta caggattggt aacgtgggta
     2221 ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aaatattaga tggagggcat
     2281 tatggaaaat aaaattcatt gcactacggg aagagtaatt actttagtgt tgtttttgtt
     2341 ccttcttctt tttgcgaggg gaactgcagt ccaggcctta gaaaaagttg atccgagtca
     2401 aatcaaagaa aataataatc tggtgcttta tcatgatagt gattcattga gaaatatcga
     2461 agtaactggg atcaatggat ttaagatact agatgagtat tcaactaaaa aacaaaaagt
     2521 acttgcttgg ccaaaagtca aagatttgaa aagtacgtca aaaatcgttg cccattatca
     2581 aaaagtaggt acttatctag gaaaagaaat tgattgtgac gtgacttttt ctaattttgt
     2641 agcgcacgct ggggttaacc aagacggatt agctaaaaca attaatggca aaaagtatta
     2701 taattatttg ctctttgata aaaatattta tcgaggattt ttcatgcagt caatcaatgt
     2761 catgaatgca aaactagaat ttaagtatgc aggaacaaaa acaaaagtag ctttgagtga
     2821 accacagcca gaaattcaat caaaaatgag atcgatgaga gcagctggta ttgatttaaa
     2881 cgatacattt ctatccattt atagtttaaa tggacagtat cagcaacgtg tgtcttggta
     2941 taatgacaat aatgaatctg tcggtgaacg taatattgat atgagagaat ttgttgggta
     3001 tgaaaaaatg ggtagcttac cttattttgt cacaacagat acagcatgtg cagaatacaa
     3061 agctcctgcg ttatcaacaa acaatttaac ttcaaaagta gtgggaggac gtgcagaaaa
     3121 ggcttatagc tcgaatgatc atttcaccga tgttgtagga gctgatactt atcacagaag
     3181 tggtgtaacg tatacgcttc aaggcgcttc cccaacattc atgattggcg caaatacgaa
     3241 tagtatgatg tttagctttg atactgcatt gctatggaca ccacaaccat cgaagcctac
     3301 aaaagaagtg tttaacaaag ctaatactga agaggcagca cacaatattg acaaaaaagt
     3361 gattccacaa ggatcagatg tttactatca tattcaccaa aaatttgatg cattaacagt
     3421 caacacaatg aacaaataca aatcatttaa aatcactgat acctttgaca gcaaaaattt
     3481 tgatatggta ttggatggga aaaactatga tggcgcattg tggtatgttg aagcgaatgg
     3541 tacttggaaa aaagctgata caggaggaaa attaactgta tcaagcaata cagtcactta
     3601 tactgcttct gacagctttc tagctaacgc tgcaaattac ggtagagatt atattttgca
     3661 gctccatatg aaagcaaaaa atgatgcaaa aggaatcgca cccaatgtgg ctactgtaac
     3721 gtttaacaat gatccgaata aagggacaca aagaacaaat acagtagaaa attatttctc
     3781 tcttaaacca aataaaaaag tggaacaaga tgggaaagat gttgatggtc gaaatgatgg
     3841 taaaaaagga acgcctacag ctcctttaaa tgcaggatct gaagtacaat atctagtcac
     3901 acaaaaatgg catacaaaag gtgtagacat tgcgctcgat cattacaagc aatttagtat
     3961 tcaagatcca gtggaagctc gtttgacgta taaagaaggt tctgcgcgag tgatagatat
     4021 gagtacagga aaagatatta cttcagaagg tacactaacc tatgatagca attcaagaac
     4081 gctgaagtgg gaagcttccg ctgatttctt aagtaaaaat cttttagatg gacgagaaat
     4141 tcaactgata tttacagcta aaactccttt gaatgaagaa ttggatatcg acaaccaagc
     4201 aaaagtttca gtagaaagta ttgagaaagc aactaatgta gtaacgattg gggtaaaacc
     4261 taacttacct cagatcattg tgccaaaaac tggatcgaat catttagtgc taactgttat
     4321 tggaagtata ttatttctta ctttagcatc attgggctat gtagttttaa aactcaaata
     4381 aatataaaaa caaggctgag aatcagcctt gttttctttt atttgaagat tggattcgca
     4441 ctaataaata cattatctac gccaattcta ccagcagagt caactacaaa attatgacct
     4501 aatccgagaa tactttggtt attataaagt aaatttgcat tatgggcagg gctatttttc
     4561 catcctacca tggcatcgat agcaccatcg tctgataaaa ggtcatcaat atacagccca
     4621 gtttgactta tgttttctcc cttagcaccg tagcagccta agctttccaa taaatcttgg
     4681 aaagtaccac caccagggcg atagtgactg aagctgactt gtaattcttt agctcgaata
     4741 tctgcagcct gttgaacaat atcattgaat tgcattgggg caacaccatt ttgttggcga
     4801 taatcattta accaagcaag ttctttttga cgcataaagt tacgtaattc gtctaatgaa
     4861 atctttttat atttgaatgt gattgtttta ttttgatcga cgttattaat ggatcgttgg
     4921 ccgtttttat ccacggtaac ggtaggatcg tcatttctgc acaagaaata attttgatca
     4981 gtcatgtttt tccaaggagt agcaaagcta tccccttcgt atactgtttt tgattcacta
     5041 gagagaacag cgttggtatc agcatttaca tgattaacag tcacattaaa tttacgagtg
     5101 tagttgaaaa tataattttg actttcagtc acattgttta aggttactga atttgttggt
     5161 tctacccgat cgtctaaaac atatccttga atagaaggag cagttaaagt gacactgtca
     5221 ccgtgagtga cattgactat ctgttctttc aaaatattaa ctcgtccttc gtttttgtag
     5281 ttgttattgc acaacaaatt gatttgatgc acttgattat aattgaatgt gattgttgta
     5341 ttacctgtga ttgttttgct ttgagtatca ttaccaatca ctttgtagct aaaattatct
     5401 ttagcgcctt cgctatattt aaatgctttt gccttagcag tgtaagtaga accagaagtt
     5461 acttttgctt gatctgtagc aatcacttga cctgtttgat tgtcaatatg gttaatagta
     5521 accgtatatt cttttggtgt ttcaggtttg tgttgcaacc ctttaaatgc agcatcaaga
     5581 ctatttttag cattatttac ttgggcttga gaagccttag aatcgtctag tactttttta
     5641 gcattggata aagcagtctg aaaattattc caagattctt ctgtaaaatc tcctttagca
     5701 gttgatttta ctcggttgta taaagcttgt aattcagctt tattcacagt aggaactggt
     5761 tttttcttta atcctttgta agcagaatct aatttatttt tggcactatc cacttgtttt
     5821 tgagtcgctt ttgagtcatt taacactttc ttagcattgt ttaaagcggt ttggaatgcg
     5881 ttccaggtat tgtcggtata gtcacctttt ttagttccct taacgctcgt ataaagagct
     5941 tctaaagctg atttattcac tttgggttta gaggtgtcag cgccatacca accgatacct
     6001 tcgtatttcc agccatagtc tttggttaac cattgtactt ctttccaact tgcagtgtag
     6061 tgatgtgagc cagattttac accaggaagg aaaaggcggt agatcggttt attatttttt
     6121 tcggcagaac gccaggctgg cccttcataa gtccagccgt aatttttagt caaccatttc
     6181 acttcatccc aactagctgt gtaatggtga tcacgaagac ctttattgta caaacgatag
     6241 acaagatggc caccgctagt cggtgcttcc caggcggggc cttcataatt tccccatttt
     6301 atctttacta gatggtcacg ttcttttttg ctagaggtgt aaaaatgttc atgattattt
     6361 ggattgtaaa ggcgatacat gacgttagtt gaagcagcgt caacagatcc agcaagacta
     6421 agtgaggcag caaatgttcc aatagctaaa acagtttttg gattaaataa tttcattttt
     6481 tcaactccta aatttaatta aaaatgttta catgattatt atatatcggc taaccgatat
     6541 agtcaataaa aaataagaaa agccttatat aattttggct tttcttattt tatttgttag
     6601 ttgaagtatt aatatcaacg atttcttcag ccaatttatt taatttcata gcatttatga
     6661 atctcatgtt gtctatacta gaacgtttgt tggcaaggtc acttactgta gttaagggta
     6721 cgccagattc tatggatata gaacgaactg atttaccact atcaaacagc aaccattcga
     6781 ttttcttttt atcagcgtac atttcaatgc tccttcttgt tttttcttta ttatagctct
     6841 tttttgtaat aaatcacaat atcaagagaa aaattttcta tagaaaggtg gatttttatg
     6901 agtattcgtt ctccgtgtat ccaaatgagg tttgttgcta ctttcgttat ttgatatact
     6961 aaaaataaac aacgaaagaa ggaataaaat gaagaaaaag caactatggt ttgtctctat
     7021 gtttgcaata ttttttatta ttttaagtgg atgttcaagt aataagaaat cggaagctaa
     7081 agaagataat agtttattgc aaccaggaac tgtttggaaa gaagaagtag gtggtcttat
     7141 tcataattta aaaattatag atgatactac ctgggaatat tctgaaagtg tatggcatcc
     7201 agatcctgta caaataacag ttaagaggca aaaagactat gaaggtttag aaagatacaa
     7261 aatagtagat tctgcaggta ttagggaatt tataaacaaa cctgattcat tatttattgt
     7321 tgttccctat gagaaggatg gcgtaaaaaa aataattttt cttggatcta gcaaggatga
     7381 gaagcgaacg aaagagaaat taattcatga tggatcagag ttaaataaat acaaattaca
     7441 aaagactagt gagtgattgc tagtcttttt atttatcctg aaggaggaat cacagtggca
     7501 aaagttaaaa caccattcga aaaagccgcg tctaaaatct tagaacgccg tggtgtttct
     7561 gaaatacagt ggaaaaaaga agttcttgaa aaagcacaat ttgacctatt acgaggaaaa
     7621 gataaggact tagaagaata tatctatcaa cgtgaacgtg aaaaacttgt attcgatgaa
     7681 attattaaac attaaaagga gcgaataatc ttatgtttga atcaattaca atgaattttt
     7741 taactcaacc gaaagtagta gctgcagttg atatttttag agcattacaa actgccgcaa
     7801 ctagtttgaa aaataatctg atgatttttg ctgctgttgc ggcagtggct tccattgctc
     7861 ttggaggtat tttcttcttc tttgggcgaa gaggagctga aaacgggaag acacacgtag
     7921 gttatgtatt gttgggggta ggagttattt tagctgccgc ttctattgta gtttcaatgt
     7981 tctcaatgtt tggacaaacc ccacaaggac tataaaaaaa caagtagaag gagtaaaaag
     8041 tacgtggcta ctttttactc ctttttttat ggaaggagaa tattcatgtt ttttacgatt
     8101 tcaggcaatg caaaattgga taaagagtta ataagaaaag caacgaagaa agtgggaaag
     8161 cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga agaacagcta
     8221 catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt gactctcacg
     8281 ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt tggctcactg
     8341 gattatcaaa tttcagaagg tttttatcct acattgattg atttaatccg cctagatata
     8401 tttaaagata cggatgaaac catgagtgtg gaagagtctt atcgaaacga aaaaatttta
     8461 caagttttag aagaagagtt agaaagcgac caaagaaaag tgaatcaagt gagtgaagtc
     8521 gaagcaaacg aagaagtacc atgggaacgc ccaatctttc caaaggatga atcaaaagaa
     8581 aaagagaaag gaattattga cgaacaagct gacattttga ctcataaatc aatatttgag
     8641 gaagagaacg aggtaatggg tcaattggag gtagatccct ttgaagaaac aatggaggat
     8701 gaagagacgg ccgaagtaga agttgatcca gttgaagcta atgaagaccc aacatggact
     8761 gaaggggatt ctcaggaata tattgaacca cttatcagtg aagaagtaga gaaaaatgaa
     8821 acagagatac aaaaggcaac ggtatttgat tatattttgg atcaatatcc gccagagcat
     8881 gaatggatga aagaaaaaaa taaattattt ttaaatgaac tttattcgaa atatcaatta
     8941 cctcaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca gggtaaaggc
     9001 acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct tgcacaagaa
     9061 gatttattag ctacttttga agaacgtcaa caagaaacag atcaagaaat taatgagtat
     9121 gcgacggaac agtcgtcgaa ttgggagaaa gcaaaagagg aactaaataa agaagaacag
     9181 cgagtgattg aagaagaagt acaaaaaatt aaagagcggt acgaaaagaa acgtgacaat
     9241 gcgtattcct tgtgtgtaga aaagatcaac aattttactc agacgaatca agaagcatta
     9301 tcgaaagaaa aagaaaactt attaaaagaa cgtataaaag aacgaaaaga agaattttac
     9361 cgagggttgc gtactgataa gctaaggatt agcaaagaat taaatgaaca actgaataaa
     9421 ctctatcaaa ctacgactga agttctgatg gaaaaacacg agcagatcca acaagaatta
     9481 caagcacaaa ttccaaaatg gaaaaaagag tatgaaattc accaacaaga attgaaagaa
     9541 caagaagaaa aacaacggaa aaaagaaaaa gagcgcgaac aattagaact aaaaaaacgt
     9601 gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggctaga gcaagaaaag
     9661 gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
//
LOCUS       DAJSMI010000097         9665 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJSMI010000097 DAJSMI010000000
VERSION     DAJSMI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.098"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..650)
                     /locus_tag="ON430_001902"
     CDS             complement(<1..650)
                     /locus_tag="ON430_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3219814.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="ON430_001903"
     CDS             975..1391
                     /locus_tag="ON430_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR3219815.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="ON430_001904"
     CDS             1388..1858
                     /locus_tag="ON430_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3219816.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="ON430_001905"
     CDS             1872..2645
                     /locus_tag="ON430_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3219817.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="ON430_001906"
     CDS             2659..3471
                     /locus_tag="ON430_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3219818.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="ON430_001907"
     CDS             3525..4490
                     /locus_tag="ON430_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3219819.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="ON430_001908"
     CDS             4606..7329
                     /locus_tag="ON430_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3219820.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANCMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="ON430_001909"
     CDS             7342..7974
                     /locus_tag="ON430_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3219821.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9665
                     /locus_tag="ON430_001910"
     CDS             8873..>9665
                     /locus_tag="ON430_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3219822.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3260 a         1476 c         1885 g         3044 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agcgacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaactgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtatt
//
LOCUS       DAJSMI010000098         9654 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJSMI010000098 DAJSMI010000000
VERSION     DAJSMI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.099"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            1064..1744
                     /locus_tag="ON430_001911"
     CDS             1064..1744
                     /locus_tag="ON430_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR3219823.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
     gene            1773..3086
                     /locus_tag="ON430_001912"
     CDS             1773..3086
                     /locus_tag="ON430_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR3219824.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            3892..4659
                     /locus_tag="ON430_001913"
     CDS             3892..4659
                     /locus_tag="ON430_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCR3219825.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            4686..5687
                     /locus_tag="ON430_001914"
     CDS             4686..5687
                     /locus_tag="ON430_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR3219826.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            5710..6996
                     /locus_tag="ON430_001915"
     CDS             5710..6996
                     /locus_tag="ON430_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HCR3219827.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            7030..7683
                     /locus_tag="ON430_001916"
     CDS             7030..7683
                     /locus_tag="ON430_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3219828.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            7716..8921
                     /locus_tag="ON430_001917"
     CDS             7716..8921
                     /locus_tag="ON430_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3219829.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VGCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
BASE COUNT         3109 a         1382 c         2263 g         2900 t
ORIGIN      
        1 aaatattcga aattttggga tgataccact cttaaatata atgatctaaa tgctttaata
       61 ttggtatatc cgatatagga actgacgagc tatttagtat gatacaaaag tatattgata
      121 gccattaata aattttccaa aaaataatga cagcacacca atttttataa tttagcttgt
      181 tgaatcggta tgttcatggg acggttcaaa aaaagaatcg aaaaaataaa cggtaaaatt
      241 ttagtgaatc ctatgtaata aaatttggat agatggaaaa acagcacata aggaggctga
      301 gtttgttgtc atttctgctc ctacaaacta tggtagtgaa tgtgttattg aattagtgat
      361 gtctgttact tcagagacat tggggataat taacttcact atacctgtag ggtatacaaa
      421 aaatggaaga aaaaatatta tcaatagatt ttatttagtt cagaattttt acgtggaagt
      481 aaattattga tgataatctt tatccaatta gaattattat cgtaggatgt gatgatgata
      541 gccgtgaagt tgttcaagta tttaccaatg tctacaacgc agaaaattag tttgggtttt
      601 tcggattgga aaatttcaat gtataaaatc ataaatttgc tgtaaataga cagtttgggg
      661 tagtaatagc ccaataaatt gttcgataaa ctcatctttg gggtgagaat agctcaatca
      721 ttgaatttca aacctgcctt tggggtggga ttagaggtca atatgtacct acataatact
      781 ttttggaaaa tgatggtgtt gagaatgtaa gtacatttca atgtgaaatt ctaatatagc
      841 cccaaacgag gtcaataaaa atgacctaaa ctcgtatttc tcccctaaac gaggaggaga
      901 tagatatgaa aattgaatgt gccggcgttg gagttggctg cttttagtag gaatgaacat
      961 ggttcgatga gaactgtttc agatagagtg gtcgatgatc acaggccttt aagtcgttta
     1021 cagatgatga gacttgtaaa aatagaggga cgaatggaaa aagatgattg gtatagaaga
     1081 aaagaaaggt gttcagaaag acgtaacaat tgatagggaa gagaatctta ggggacatag
     1141 attctattgg attggacgca gagcacaaga tatcttcttc tcggctctag ctttgattgc
     1201 attgagccct gtgatgctaa ttacagcgct cgccattgtg attgacgatc ccggtggcag
     1261 cccgattttt gtgcaggata gagttggaag aaatggaaag ctgttcaagt tttataaatt
     1321 ccgatctatg tgtgtagatg ctgaagcaaa attagaagcc ttgttggcac aaaacgagat
     1381 ggatggtccg gtcttcaaga ttaaagatga cccaagaatc acgagagttg gtaaatttat
     1441 tcgaaagaca agtatcgatg agttacctca gctctggaac attcttaaag gtgatatgag
     1501 tattattgga cccagacctg ctttaccgag agaggtggaa caatacggtg actacgaaag
     1561 gcagcggttg ttcgtacagc ctggtcttag ttgctattgg cagattgctc cacatcgtaa
     1621 tgaactgtcc tttgaagaat gggtggaact tgatgtgaag tacatcaatg aaagaagctt
     1681 tatcacggac tggaagatta tcttcttgac tttacaggct gtattatttg gatatggtga
     1741 atgattatat atagtattag gagttgaaat atgtgaatat tattttatta tcgggtggta
     1801 gcggtaggcg gttatggccg ctgtccaacg atattcgtag caaacaattc atcaaactgt
     1861 ttaaggatga aaatggcgaa tatgaatcta tgattcaaag agtttatcgc cagattacaa
     1921 ctgttgaccc agatgcaaag gtcactattg caacaagtaa gtctcaggct agtgcaatcc
     1981 ataatcagtt gggagataag gcttcgattt gtgtagaacc ttgtcgtaga gatactttcc
     2041 ctgcaatttt gttagctgca gcatatcttc acgatgaact tggcgtaggt gatgatgaaa
     2101 gtgttgcggt ttgtcctgtt gatccttatg tagataattc ctattacgaa ggcgttgaag
     2161 cccttgagaa gttggtgcag cagaatacgg ctaatttgac tttgatgggt attgcaccta
     2221 catatcctag tgaaaagtac ggttatatca tccctgaaag ttctaacgaa gtaagtactg
     2281 ttaaggagtt taaggaaaaa cctgacaaag caacggcaga gaaatattta gctcagaatg
     2341 ctgtttggaa tgccggtgta tttgcgttca aacttggtta cctgttggaa aaagctcacg
     2401 caatggtgga ttttgaagat tatcgtgatt tgtattccaa gtatgagact ttaaccaaga
     2461 tcagctttga ctatgctgtg gtcgaaaaag aaacgagcat tcaagtgatg cgttataacg
     2521 gggattggaa agatgtcgga acttggaaca tgatggctga agttatggct gataatatta
     2581 aggggaaagc tgtaatggat gatacctgtg ttaatactca gattgtgaat gagttgaata
     2641 ttcctattct tgctatgggc tgcaaagata tggtaattgc agcaagtggc gatggaattc
     2701 tgatttctga taaagagcgc agtggctata tgaagccgta tgttgagaaa atcagtacgg
     2761 aagtaatgtt tgcagaaaaa tcctggggta catataccgt tatggacgtt caggaaggct
     2821 ccatgactgt taaagtttct atgcaggccg gacagaagtt gagctaccac taccacaatt
     2881 ctcgcgatga agtttggact gttgtagcag gacagggtaa agcgattgta gatggtatga
     2941 agcagattct tcgtgcgggt gatgtggtga ctattgccgc aggatgtaaa catacaatcg
     3001 aagccgtgac aaatctggat attatcgaag ttcagattgg tgaaactatt agcgtcgctg
     3061 acaagtataa gagttctttg aactaaaagt tcgcgaggta gttattatgg agatactaaa
     3121 atttcaaccc gtgggtaaag attatctttg gggtggagaa cggtttaaga cggaatttgg
     3181 aaaaaattga cttaacacca ttaagcttat acgtgggaat gcttagttca tctagacgat
     3241 aactacgtac ggaaatatca aatatgaagg acagaatggt aaaaccgagt tgtggtatgt
     3301 attggaagct acaccggggg gtgatttatg cttttgagta cgatgtaaca gaagaaatgt
     3361 tacgaaatgc tgtgatggac agatcacttg ggaaacattt atataaagaa gaagtacaca
     3421 agggagatct tttttatgtc agttcatggt attggcgctg gagtattagt ggctgaaata
     3481 caggaaagct caaatgttac ctatcgagtt tacgattatg atcgagtaga taagaatgat
     3541 aataagtgag aattgtactt tgaaaaagcg attcaggtaa tgaatatgga agccactttg
     3601 aaattggaga aaagaaataa gctcattcat ttttatcctg gtggctctcg tgaaatactg
     3661 tgtagatgta aatactgtga gcccgaaaga atccaagtaa agacggggtt ttcgttctcg
     3721 gtaaaagagg aatcttttca agttctttta tgtatatcgg gggacggaca agttgagtcg
     3781 gcaaatggtg ttatgaaact cattagattt gtaaaaaaga tattgtatgt ttttgccaac
     3841 gaatattgga agatgttaag tgttgagcga tgcggactta ctgaaaatta gatggggata
     3901 gagggaggat atctaatgtc acgaatgaga tttatgaaca cagaaattga caatcttaca
     3961 atgggtgaag tattggtaga ggttgaaaaa atgattcaag ataggagatg cggatatgtt
     4021 gtaactccga atgtggatca cattgttcag ttggaaacag gaaaagagtt gcaagatgtt
     4081 tataaaaacg catctttaat tttgacggat ggtaaaccat tgatttggat ttcaaagttt
     4141 tataagacac caataaaaga aaaaatatcg ggatccgata tattttcttt actgtgcaag
     4201 atggctgcag aaaagggata cacaatgtat tttttaggtg cggcagaggg cgttgcggct
     4261 aaagcggcag ataacctgat gaaaagattt ccgggattaa atgttgtagg aacatactct
     4321 cctccttatg ggttcgaaaa agacgaagat gaaatgttta aaattgattc ccaaattaaa
     4381 cgagcaaatc cagatatgct tattgtgggt ttgggttgcc ctaaacagga aaaatttatg
     4441 ttttataact gtgatagatt gggagtacct gtgtcttttg gtctaggagc tagtttggac
     4501 tttgaggcag ggaatgttaa aagagcacca aaatggatgt cagattatgg atttgagtgg
     4561 ctgtttagaa ttactcagga ccctaaaaga atggcaaaac gatatttggt tgatgattta
     4621 aaaatcttta aattagcact aaaatatcgg aaaaaataaa ctatattcaa tgaggacaat
     4681 gaagtatgat tattacaaaa acaccttttc gcatgtcttt ttttggcggc ggaacggata
     4741 tggaagatta ttttcgagaa aatggcggtg cagtgctttc cactactttt gataagtact
     4801 gttatgtgaa tgtgcgtcat ttgcctagat tttttgatta cagcacagag ttatcatatt
     4861 ctcgtactga gagagtaaca aatattgaag atatcgagca tcctgcaatt cgtaatgcga
     4921 tgaaaatgct agatatgcat gaaattcgcc ttacatatga agcagatctt cctgcacgtt
     4981 ctggtctggg tacatctagt tcttttgccg taggtatgtt gaatgctttc tatgctttga
     5041 agggcaagta cgctgacaag aagaaactcg ttgataaagc aatttatctt gaaagagtcc
     5101 tttgtaatga agcaggcgga tggcaagatc agattgcagc agcttatggt ggctttaacc
     5161 gaatcaattt caactcagat ggctatgaag tattgcctat tattattacg cctgagcgta
     5221 aaaagcagct taacaacaac ttgatgatgt tctttactgg attcaccaga ttttcctctg
     5281 atgttcagaa ggcaaataat gtcagcggca cagaggacaa aagagtaaga ctgaaaaaaa
     5341 tgtatgagct tgtggatgaa gcagaagcgg ttctgactga taagaacaga gatctagatg
     5401 atttcggcag actgcttgat ctcacttgga gattgaaaaa ggctaccggt ggtgcgatta
     5461 gcactggaag tatcgatgaa ctttatgaaa agggcatggc tgctggtgca ttgggcggta
     5521 aacttctcgg agcaggtggc ggcggtttct tggtttttta tgttcagcct gaaaaacaaa
     5581 atgctgtgcg ttcggcaatg agtaatttga tgtatattcc gtttgaattt gaaaacggtg
     5641 gaacacaggt tattcattac acaccggaaa tgtatattcc aaaataaaga aatcagcgag
     5701 gataagaata tgaaagttgt tattatggca ggcggtaagg gaactcgcat ctcatcggtt
     5761 gcaagcgata ttccaaagcc catgattaaa attgaaaaca aaccggtatt ggagcacgaa
     5821 atcgaatgtc tgagagacca gggattcaca gatattattc ttaccgttag ccatcttggc
     5881 gatatcatta tgaactattt tggcgatggt tctggaattt caccagttac tggtcagccc
     5941 tttgacgtta aaattgaata ctttttggag aaagagccat taggaaatgc cggtgcattg
     6001 tttcgtttaa aggataattt gaccgaagac ttcctcctgc tgaatgcaga tgcgatgttt
     6061 gacgttgatt tcaaccgatt tgtagcatat caccgtgaaa agggtggctt ggtgacattg
     6121 tttactcacc ctaacagtca tccatatgac agcggtctaa tcattgcaga taaggatggc
     6181 tctgtagaca gatggcttgt aaaggaagat tcccgtccaa cttattaccg taacagagta
     6241 aatgcgggac tccatgtgat tagtcctgct gttcttgatg tcgagataaa cacacctaag
     6301 attgacttgg accgtcagtt attaaagccg ttggcaggaa ccggaaaaat gttctgctat
     6361 gatagtccag aatacgtaaa agacatgggt acccctgatc gttattatgc tgtctgtgaa
     6421 gattttaagg ctggaagagt gaagggcaaa aatcttcaga ataagcagaa agctgttttc
     6481 cttgatcgtg atggaacgat taatcaatat gttggctttc tgcgtcatat tgatgagttc
     6541 gaactaatca agggtactga agatgcagtg aaaaagatca atgccgctgg atatctggct
     6601 attgtaataa cgaatcagcc agtaattgca agaggtgaag tttcttttga agaattggaa
     6661 gagatccata acaaaatgga gacacttctt ggatataagg gcgcttattt ggatgcagtt
     6721 tattattgcc cgcatcatcc acataaggga tatgaggaag agcgcccaga gctaaaattc
     6781 gattgtgaat gcagaaagcc aaagcccgga atgttaattc aggcagctac ggacttcaac
     6841 atagatttga gtcagtcttg gatgattggc gatggcgaga atgatattaa agcgggccaa
     6901 gccgccggct gtaagactgc attggttggc gagagtgagt atggacagga tatgaccata
     6961 gcaaccctga gtgaatttgc agataagatc tgctaaggga tgttttatca cgaaaggaga
     7021 gtataaacaa tgatagacaa tagattacag aaacacattg atttgctgat taaacgctat
     7081 cccactcttg aagtaatcaa gcaggatatt gttgacggat acctgattat ggaggaatgc
     7141 tatttaaatg atggcaagct gctgatagca ggaaatggcg gatctgctgc tgattccgag
     7201 catattgcgg gtgagctgat gaaaagcttt aagacgcctc gccctgttag tagggaattt
     7261 gcagataaat tgattgcggt tgatcctgta aagggttctg aattagctaa gaatcttgaa
     7321 tatggtttga tggcgattcc tctcgttgct catgaagcgc tttccactgc gtatattaat
     7381 gatgttgatg gcctaggtgt tttcgcacag cagttgtttg gatttggaag acctggcgat
     7441 gtattccttg gtatttcgac atccggcaat agcaaaaatg tgataaatgc aactgttgtt
     7501 gctagagctc ttggtatgaa agtaattggc ttaactggag ccaaaggtgg agaacttgca
     7561 agggtagcag atgtttgtgt taaagctcct gaaacagaaa catatatgat tcaggagtac
     7621 catttgccaa tttaccactg ttggtgtcta atgttggaag ataaattttt tgggaataga
     7681 taaaatatct aagcccatat tgaaaggata aaattatgaa tgtactattt ataacaactc
     7741 aatttccata tcctctagat aatggcggaa aaattggtgc gtacaacggt atcagtgttt
     7801 tatctagaga ctacgatgtg acggtattgt ctttcaccga agaaccgcag tatgttgaag
     7861 aaggtgtcag tttttttcga gagaaattta atagtgtaaa atttgaaaat cctgtgatac
     7921 atgatgtaca cataagaaat aatccaatga aattggtaga agctttagca atagggtatt
     7981 gtctacgcca accttatata gtttcaaagt tttatgattc aaaaatgctg cagttaatag
     8041 atgaaaaatt ttctaacaat gcatattggg atatcatttt tattgactat ctgaatatgc
     8101 aaagttatgg tcaatacata cgggagaagt ataaaacaca atttggtaag tatatcttaa
     8161 aagaccacaa catagaatat gatttagtcc tacaggaagc tgaatcaagt cgaggtttaa
     8221 aaaagaagtt gcttcagtta gagtataagc gtactcgaaa gtatgaagag gccgcagtaa
     8281 atagggccga ttattgcttt agtgtgtgtg atgcaaatac ggaagtactg aaaaagatta
     8341 atgacaacag ttatacgatg cttccaactt atgaaatgct tacaggggtg aaaaaaaata
     8401 tagagaagaa cacgatccta tatttgggta acctttcttg gaaatccaat atgcaaggat
     8461 tagtttggtt tttagagtca atttatccaa atattcaaca aagaattccg gatgtaaaaa
     8521 tcacaattgt cggtagcggg ccagttggaa cagattttag taaatattca ggcgtggatt
     8581 atcgtgggta tgtaaaagat atttcacaca tttatgacga tcaagcggtg ctaattgtac
     8641 ctttgttaga aggtagtggt ataagaataa aaatattaga agcgttcaat aatgaaattg
     8701 cagttgtatc tacattagtt gggtgtgata cgattggcgc aaaagatgga agtgagttat
     8761 ttatagcaga taacgaaaaa gattttgcaa cggctgtagt tagactgctt gaagacatga
     8821 atttgaatga aaaagtaaga aagaatgcaa aattattctt aaaagaaaaa tactccttaa
     8881 cggctagaca agatgagttt agtgcgataa ttaacagcta atatatatat aagcttatct
     8941 gatgatgatt tataggagga aatatatgga gatggctgag agaaaggaaa agaaacttgg
     9001 aaataagaat atcatattat tttagaaaac tcctgttatt ttatgtttgt gtgattgttg
     9061 cggctatgag ttaccacctt acaggcaatt ccaatgtagc gtcattaatt gctgtagtag
     9121 gagtagttta tgctattgta aaggcgtgta ataacgagta tctggctata tgggttatgt
     9181 ttgtattagg ttctgatata tgggggatag tgccatatat taacattggt agtggtgtgt
     9241 tgcattggtc agattttaat gttatagtag cgatatttct ctgcttgaga ttcatcaaag
     9301 ttaaagctag gagtagatac gggattgtct atagtgtaat gcttatattt attttgattg
     9361 catgtgtgca atcgcattca atctatggac aagatgtaca tacaacgctc ttaacagcta
     9421 gagaattgct agttgtaata tccttttggc cgatagtaaa aatagtatat tcagaaagaa
     9481 taactaaaaa tgaattcatt gatttagtgt gtaaattcac aagatggaca atgtgtatat
     9541 attggtttga atggattctt gttaatatgg gattcaatat aaccttttta cagacaggtg
     9601 cacgctgggg tactagaatc tatatagatc cgatatttat cattttatat ttct
//
LOCUS       DAJSMI010000099         9639 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJSMI010000099 DAJSMI010000000
VERSION     DAJSMI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.100"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            204..683
                     /locus_tag="ON430_001918"
     CDS             204..683
                     /locus_tag="ON430_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219830.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            716..2944
                     /locus_tag="ON430_001919"
     CDS             716..2944
                     /locus_tag="ON430_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3219831.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3073..4716
                     /locus_tag="ON430_001920"
     CDS             3073..4716
                     /locus_tag="ON430_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3219832.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4789..5334)
                     /locus_tag="ON430_001921"
     CDS             complement(4789..5334)
                     /locus_tag="ON430_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCR3219833.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5357..5842)
                     /locus_tag="ON430_001922"
     CDS             complement(5357..5842)
                     /locus_tag="ON430_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3219834.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5832..6644)
                     /locus_tag="ON430_001923"
     CDS             complement(5832..6644)
                     /locus_tag="ON430_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCR3219835.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6783..8315)
                     /locus_tag="ON430_001924"
     CDS             complement(6783..8315)
                     /locus_tag="ON430_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR3219836.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIISLSPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8424..9455)
                     /locus_tag="ON430_001925"
     CDS             complement(8424..9455)
                     /locus_tag="ON430_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3219837.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3218 a         1800 c         1860 g         2761 t
ORIGIN      
        1 aatattccaa catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag
       61 atggtgtttt tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc
      121 tataatgtcg catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa
      181 taaaaggatg aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa
      241 tcgttgtaga agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag
      301 ggctttcact gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg
      361 tatggcgaca tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta
      421 ttttagattt agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt
      481 atgaaaaatt ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc
      541 gaatgacggt agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt
      601 acgatacact acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc
      661 ttttagatga gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga
      721 gaatttctca ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa
      781 gaaaccgaaa agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg
      841 gaattggcaa gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc
      901 ggggaagaca caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt
      961 gacctgtttt gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga
     1021 ttctaataag gaacaagacc aagaattact agaagctatt taccaaacag atacacttga
     1081 agctgtactg acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa
     1141 attagcagag ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta
     1201 ttacccttct gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc
     1261 gactgtcatc cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg
     1321 gatgttgaat ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac
     1381 tacaaatcga aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca
     1441 acatgcggaa aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa
     1501 aactatgatc caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga
     1561 tgaaactgca aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg
     1621 ggcaaatacc tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta
     1681 taaattatcc actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg
     1741 attgctactg gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga
     1801 gcatacaaaa gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa
     1861 tgctgaagaa attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga
     1921 gggagaagag aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa
     1981 agaacgcagt agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc
     2041 tcaagaaatg tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa
     2101 taaagaagcg cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca
     2161 ccgaccagct ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc
     2221 aatagtagaa tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac
     2281 gtaccaaaaa ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga
     2341 tttgagaaat tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt
     2401 acggatcgat gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc
     2461 agcgattgcc attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga
     2521 tacagcagac cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga
     2581 cggatttcaa tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga
     2641 tggtgctgca cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact
     2701 gttgcttaat attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc
     2761 aaaagaagat atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc
     2821 cgcagctatt ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt
     2881 agatattact gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct
     2941 atgataaaat aaagctcagc gaacgcactt agcagattcg ttgagctttt cgtttctagt
     3001 taccggtaac aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag
     3061 gagtgaaacg atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc
     3121 tatcctgcta gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt
     3181 gaaaaactta attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat
     3241 caatctccat ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga
     3301 ttcggaacga tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa
     3361 gtgattatgg atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg
     3421 aaaaaatcta aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc
     3481 aaagaaccga ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa
     3541 actggacagt attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc
     3601 gagaaattga aagaagaact attcaatatg atccgatggt ggttggataa agggatagat
     3661 ggatttcgtt tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca
     3721 gagaaccctt tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg
     3781 aaacatgttt ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca
     3841 gaagaaggac cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat
     3901 catatccata tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat
     3961 gcattaagcg cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa
     4021 aaccatgatg ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct
     4081 atgtctgcaa aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat
     4141 caaggacagg aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca
     4201 gtagattcga aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca
     4261 ttggatatca ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg
     4321 gaacaatatg ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa
     4381 acgatcaatg tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac
     4441 atgatccgca ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt
     4501 catgaacatc cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt
     4561 atggtaaact taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc
     4621 tggacattac tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca
     4681 ccttatgaag ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag
     4741 ttggactttg cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc
     4801 tctaggacca ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag
     4861 ggttttcaaa cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt
     4921 gtcaccgata tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa
     4981 ctggtcaaaa gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac
     5041 aatttcgaaa ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg
     5101 ttcacaacct tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg
     5161 gatgatccat tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc
     5221 gcttgtgctg catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat
     5281 gtcattaact atttcagtca attggtcttt taattctttt tctgacaact tcattttcta
     5341 aaaccttctt tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta
     5401 taggaaaggc aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca
     5461 ctccagccgg ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta
     5521 agcttcctaa taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag
     5581 caacatatcc ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg
     5641 aaatcaaatc gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat
     5701 aaagaaaagc agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg
     5761 gtacagggat caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga
     5821 tcgttaattg cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca
     5881 cttccggtat gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta
     5941 ctttctgcta ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa
     6001 gaaaaggcac aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc
     6061 tcataatgat caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg
     6121 cctttgatca caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt
     6181 tctgttaacg aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc
     6241 gtaatcatcg taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg
     6301 atcacagctt cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt
     6361 tttgttttta tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt
     6421 ttgataccat ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc
     6481 aaagatatag attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag
     6541 gcgcttagtc cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca
     6601 ccagcaaatg gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg
     6661 aataagagta taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc
     6721 atccaattta aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat
     6781 agttaggact tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta
     6841 gctgttcgcg ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca
     6901 tggacttgct cccaccatcg attccaatcc atttctttat caggagataa agaaataatc
     6961 ggttgtggag aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa
     7021 tctaataaaa agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc
     7081 cgatctttca aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac
     7141 caatcgccat aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt
     7201 cctttagaca gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa
     7261 tctgtaatcc gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt
     7321 gttttcaaac tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca
     7381 tatacttgaa gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc
     7441 tggttgttta tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga
     7501 tgctccttag cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt
     7561 ggccggtgag cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc
     7621 ataggaaatc ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga
     7681 gagatgtgaa aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat
     7741 ttcgtccgcc atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta
     7801 ccagaagtat gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac
     7861 aaagcaggtt gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa
     7921 atttctctca ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga
     7981 tcgataaata cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca
     8041 ccaaggtaag acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata
     8101 ataactttgt ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca
     8161 ctttcgcagc attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc
     8221 tcatcaaaga taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat
     8281 aagttcaaag gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag
     8341 taatatacaa attcaagata atcgtaacat aatcctttat ataataacat agtttattgt
     8401 gaaaaaaaga tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg
     8461 tgataagaaa aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat
     8521 cgattaccac ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa
     8581 cgatatgttt ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa
     8641 atttggcgaa tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa
     8701 ttttcgtccg tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct
     8761 tttggtattg gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg
     8821 gtcaaagata catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac
     8881 agaagaatgg cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct
     8941 ctgcttgttt gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct
     9001 gcatcgatat atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa
     9061 atctcttctt caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat
     9121 tgcttccttg aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct
     9181 gtagtttgga acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt
     9241 ttttctaaat ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa
     9301 tcagtaaaca gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa
     9361 cgattttctt ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac
     9421 ttttcttgca gttgttttct agttttgctg tccattacaa attccctcca atagtgttca
     9481 ttttctatat tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga
     9541 agcaaatttt taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt
     9601 attttattag tgcagtcatt cttttgatct tttaattga
//
LOCUS       DAJSMI010000100         9579 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJSMI010000100 DAJSMI010000000
VERSION     DAJSMI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.101"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            23..646
                     /locus_tag="ON430_001926"
     CDS             23..646
                     /locus_tag="ON430_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219838.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="ON430_001927"
     CDS             1096..2145
                     /locus_tag="ON430_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3219839.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ON430_001928"
     CDS             2142..3023
                     /locus_tag="ON430_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3219840.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ON430_001929"
     CDS             3026..3796
                     /locus_tag="ON430_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3219841.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ON430_001930"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ON430_001930"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR3219842.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ON430_001931"
     CDS             5107..6270
                     /locus_tag="ON430_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3219843.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKEFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ON430_001932"
     CDS             6387..6797
                     /locus_tag="ON430_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3219844.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ON430_001933"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ON430_001933"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3219845.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..>9579
                     /locus_tag="ON430_001934"
     CDS             9370..>9579
                     /locus_tag="ON430_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219846.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLL"
BASE COUNT         3327 a         1629 c         2066 g         2557 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaagaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttg
//
LOCUS       DAJSMI010000101         9328 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJSMI010000101 DAJSMI010000000
VERSION     DAJSMI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.102"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..391
                     /locus_tag="ON430_001935"
     CDS             <1..391
                     /locus_tag="ON430_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR3219847.1"
                     /translation="KNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSDKEGHFEI
                     TGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASVPMTGSNG
                     FQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            406..1158
                     /locus_tag="ON430_001936"
     CDS             406..1158
                     /locus_tag="ON430_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3219848.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            1174..1929
                     /locus_tag="ON430_001937"
     CDS             1174..1929
                     /locus_tag="ON430_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219849.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNGETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            1942..2202
                     /locus_tag="ON430_001938"
     CDS             1942..2202
                     /locus_tag="ON430_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219850.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            2199..4289
                     /locus_tag="ON430_001939"
     CDS             2199..4289
                     /locus_tag="ON430_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3219851.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            4316..4780
                     /locus_tag="ON430_001940"
     CDS             4316..4780
                     /locus_tag="ON430_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219852.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            4777..5019
                     /locus_tag="ON430_001941"
     CDS             4777..5019
                     /locus_tag="ON430_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCR3219853.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            5037..5339
                     /locus_tag="ON430_001942"
     CDS             5037..5339
                     /locus_tag="ON430_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219854.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            5409..7472
                     /locus_tag="ON430_001943"
     CDS             5409..7472
                     /locus_tag="ON430_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219855.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            7472..>9328
                     /locus_tag="ON430_001944"
     CDS             7472..>9328
                     /locus_tag="ON430_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR3219856.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAG"
BASE COUNT         3491 a         1479 c         1867 g         2491 t
ORIGIN      
        1 taaaaacgca gaaggaaaat attatggtgg attagttgat acggataaag acggtgtcaa
       61 agaagcagta tgggtagacg atgtagcaaa tgcagcgatc ctaaaatcag ataaagaagg
      121 tcactttgaa atcactggtt taactgaagg tgaatattca ctggaagaaa caaaagcgcc
      181 tgaaaactat caaaaattaa cgaaagagat ctctttcaaa gtagacaaag attcatacaa
      241 agaagaaaac cgaatcacga tcaaaaacaa tcaaaaagct tccgtaccaa tgaccggtag
      301 caacggtttt caaacgtatg tattaatctc ttgcttgttg ctaggagcag gggcactttc
      361 tgcagttgtt tactttaaga agaaagccta gaaggatcaa aaccaatgat tatcaggaag
      421 cctaagaaaa agcgaatcat gggtaaatgg atcattgctt tctggttact ttcagctgta
      481 ggtgtcttac ttttgatgcc tgctgaagcg agcgtggcga aataccaaca aaatcagcag
      541 attgccgcaa tcgatcgaac aggaacggca gcagaaacgg attcttcttt ggatgttgct
      601 aagattgagt taggcgatcc tgttggtatt cttacaattc taagtatttc tttaaaacta
      661 ccaatttatg acggaacgtc cgacaaaatt ttagaaaatg gtgtaggtat tacggaagga
      721 actggtgata ttacaggagg aaacggtaaa aatcctttaa tcgcaggtca tagtggactt
      781 tataaagata atctttttga tgatttacct tcagtaaaaa agggagaaaa gttttatatc
      841 aaagttgatg gtgagcagca cgcttatcaa atcgatcgga ttgaggaagt gcaaaaagac
      901 gaactacaaa gaaactttgt cacttactta gaatcaaatc ccaatgaaga tcgagtaacg
      961 ctaatgacat gtacgcctaa ggggatcaac acacaccgtt ttctagtgta tgggaagcga
     1021 gtaaccttta caaaatcaga attaaaagat gaagaaaata agaaacaaaa attatcttgg
     1081 aaatggcttt taggaagtac tgtttttctt tcagtcatga tcattggttc gctatttgtt
     1141 tataaaaaga aaaaataaag gagggtaacc aacatgagaa atagaaaatg gctatttgtt
     1201 tgtgcagccg ctcttttttc tttcactgcc tacacacagt tgacgtttgc agacagacaa
     1261 gaaagcacgg atagcacaga acaaacaatc caaacgtcag atagcagcac gattcaaagc
     1321 agcgatgaaa ccgtcgattc gtcaacagtt gattcttcag aagaacctaa aattgctgaa
     1381 gacgatcaaa tagtcaaaca gaccattcac gttcaaaaga ttatttcttc agaacaatta
     1441 gacaaccagg ggcatgaaaa acaagaagga gtaaatggcg cgaagttttc tgtttatgat
     1501 gtatcggaca ttttacaaaa gatggatgtt aaggatctca ctactgacca aattgaatcc
     1561 caattaaaaa atcgagtgaa aaaactatcc agtgatcaat tgaagctagt ttctaatgga
     1621 gaaacaaaga caattgatca acaaacaggt gtctttgaat tctcggtaga agtacaggca
     1681 aatcagaaac aagcttatta tattgtcaat gaatcaagtc ctgaaaacat ttctaatagc
     1741 gaggatattt tgctattaac acctgtttct gataaaaacg gtgaattctt aaaggatgtg
     1801 tggatctatc ctaaatcaga agcttcgcaa cctaaagaag aagtaaaaaa aatcgtttct
     1861 actggcgtaa agaaaaactt ctttgagaat tgttgggatt ttgttactca cttgttttct
     1921 agagattaag gaagaaataa aatgaaactg atcaaattat tattattcaa tttttcttgt
     1981 ttaaccgcag tctttttgta ctgcggttta ttattttcta aactatctat tttgccaagc
     2041 taccaactat acctaatgag gattttaaac ataccagctg cttccatgcc gctagatatt
     2101 atggaaaagc tcattaggaa tccattattt actacgatca atttgatcgt tattgtcgga
     2161 atttcactag tcatttataa aaaagagggg atgaagggat gaattttaaa cactcgtgga
     2221 caagacggtt aaaatatgtt tttcccataa tgatgatgtt aggtacattg tttgggttaa
     2281 aaacagccaa tgtctttgct gaaaaagtta ttgtcgatcc gtcaaatcca atccagaacg
     2341 taaagaataa ttttattatt ccaaaagatg ttcctaatgc aaaaacaaat attactgtaa
     2401 acggagcgtc aaaagtttac aaatatcatg ttgatgccaa caaaggtggt tttacagacg
     2461 actacgtagg attagtggag ggaaacaaaa atatacgcta tccttctttc caaaaagaat
     2521 acggtgaaac aaaccttgtt ttagaaggcg taacaaccaa caccaaagtc aatattgact
     2581 atggaaaaat cggtacgtat aacgggaaaa aagtcaatat caagctagtt ctatctaata
     2641 ttcacttata ctcggatacg ttgccgtgga atctactaga taacaactat acaaaaactc
     2701 attttcgaga cgatggctat aaaaatacga atggtgcaat gagtaaatct aaaaaaagaa
     2761 cagtactttg gatctcagac aatcttttct caggaattgt ttaccattca acacaaatga
     2821 atgtgcagct agtagccaca tatgaggacg gtagccccgt tcaattttca ggagatacat
     2881 ttatcagttt taactctttg aaccctgcag gaggaaaaag cacggatctt aaaggagaat
     2941 atgcccacta tgacaaaatg aatacgacag attggtatgt cagaaaagat acggttcttt
     3001 cagaatttaa atctttctac aacaatctga acgtggtagg tggccaccca ggaggaagct
     3061 ctaagctgac acaagcggat aatgatttca ataatttgca tgataaatta ggagatccga
     3121 agtttggtca aggtactgtg tcctttaaaa tatctgaagc aaatccgacg tttgtgattg
     3181 gttcttctaa tgttcaaacc tggtttactt tatcttctgc gacaatcttt agtgttgttc
     3241 ctgatcaacc tgaaaaaaca ggggttgata aaaatggaaa taacgtgaac gataaaatgt
     3301 tgcaagtagg agacacgatt caatatcgaa tcaaacagaa ggtgaatcga ttaggagtgg
     3361 atttactagc ggtttacgat cgatttgagt taatcgataa tttaccaaaa gaagtaaatt
     3421 acgtcgatgc acacgttgaa agtggtacaa ataaaaaatt cgatgtatct ggtgaagtga
     3481 cctatgacaa aacaaaacac caggtaaaat atgcagcgaa agctgataca ttaaaaaatc
     3541 gaatgaagta taacggcgaa acttacgaat tagtgatcaa tgtaaaagtc aatgaattgg
     3601 ccaatcaaaa tagtgtagcc aaaaatcagg gaacgtcgat cattaataaa gtagaaaagg
     3661 atacaaatat cattaccgtt tatttcccta aaattccagt gaaagaagta caacaaaatg
     3721 gaaaagatgt aaacggacga aatgacggta agaagggtac gccgacagct cctttaaacg
     3781 caggatctga agtacaatat ctagtcacac aaaaatggca cacaaaaggc gtagacgctg
     3841 cgtcagatca ctacaaacaa tttagtattc aagatccaat tgaagctcgt ttgacatata
     3901 aagaaggttc tgcgcaagta atagataaga gtaaaggaaa agatattact tctgaaggca
     3961 cactaaccta tgatagcaat tcaagaacgc tgaagtggga agcttccgct gacttcttaa
     4021 gtaacaatct tttagatgga cgagaaattc agctgatatt tacagctaaa actccattac
     4081 aatcagaaaa aaatattgat aaccaagccg tagtagcagt agataatgtt tctaataaaa
     4141 caaatgttgt gacaattggg gttgatccta acttgccaca ggtcattgtt cctaaaacag
     4201 gttctacaca tttagtaacg atttcagcgg tcagcttact attacttgtg ttagcaactt
     4261 ttagttatgc ggttctgaga tacatttaaa ataaaaaaac gaaagaatga ataaaatgaa
     4321 aaagttaata atagtagtta cttccttgat tgccatgatt gttattttag tcggatgtag
     4381 taatgaaaaa aataaacaaa ctaatcaaga taatggcgta ttgaaatcag gaactatgtg
     4441 gaaagaagaa gtaggtggtc ttgtttataa tttgaaaatt atagacgaga ctacctggga
     4501 atactctgag agtgtatggc atccagatcc tgtacaaata acagtcaaga ggcaaaaaga
     4561 ctataaaggt ttagaaagat acaaaatagt agattcagct ggtgttaggg aatttataaa
     4621 caaatctgat tctttattta ttgttgttcc ctatgagaag aatggcgtaa aaaaaataat
     4681 ttttcttgga tctagcaagg atgaaaagcg aacgaaagag aaattgattc acgatggatc
     4741 acaatccaat aaatataagt tacaaaagac tagcgagtga ttgctagtct ttttatttgt
     4801 cctgaaggag gaatcacagt ggcaaaagct aaaacgccat ttgaaaaagc cgcgtctaaa
     4861 atcttagaac gccgcggtgt atctgaaaca cagtggaaac aagaagtcct tgaaaaagca
     4921 caatttgacc tattacgggg aaaagacaag gacttagaag gatatatcta tcaacgtgaa
     4981 cgtgaaaagc ttgttttcga tgaaattatt aaacactaaa aggagcgaac aattttatgt
     5041 ttgaatcaat tacaatgaat tttttaacac aaccgaaagt agtagctgcg gttgatattt
     5101 ttagagcatt acaaactgcc gcaactagtt taaaaaacaa tctgatgatt tttgctgctg
     5161 ctgcggccgt tgcttccatt gttattgggg gtcttttctt cctatttgga cgaagaggag
     5221 ctgaaaatgg gaagatgcac gtaggctatg tattgatggg ggtaggagtt attttagcag
     5281 cagcttctat tgtggtttcg ttatttggat tgtttggaca aacacctcaa ggattataag
     5341 aaagtaaaaa agtaggagta agaagtagtc gatacttttt actccttttt tatgaaagga
     5401 gaatactcat gtactttacg attgcaggca ataccaaaac agataaagag ttaataaaaa
     5461 aagcaacgaa ggaagtagga aagcgtactt cttttttacc gaaaacaatt aaatattctg
     5521 tcattgaaac ggaagaacag ctacatgtgt tagggaaagt actggaacac gaaagtcgat
     5581 cttctgttaa gttgactctc acgttacatg acacagaagg aaaaatagag aaagctggta
     5641 cagaatacgt ttttggccca ctggattatc aaatttcaga aggtttctat cctacattga
     5701 tggatttaat ccgccttgat gtgtttaaag aaacggatga aactatgagt gtggaagagt
     5761 cttatcgaaa cgaaaaactt ttacaagttt tagaagaaga gttagagagc gaccaaagaa
     5821 aagtgaatca agggagtgaa gtcgaagtaa atgaagaagt accatgggaa cgaccaatat
     5881 ttccaaagaa ggagtcaaaa gaaaaagaga cagtctatgt tgacgaacaa gctgatattc
     5941 cgactcagaa atcgatattt gaggaagaga acgatgtgat gggtcaattg gaggtagatc
     6001 cctttgaaga aacaatggag gatgaagaga cggccgaagt agaagttgat ccagttgaag
     6061 cgaatgaaga gtcaacatgg actgaagggg attctcagga atatattgaa ccacttatca
     6121 gtgaagaagt agaaaaaaac gaaatagaga tacaaaaggc aactgtattt gattatattt
     6181 tggatcaata tccgccagag catgaatgga tgaaagaaaa aaacaaatta tttttaaatg
     6241 agctttattc gaaatatcaa ttaccccaaa cctatcaagc atttttagag agtaaagaag
     6301 aattaatctc acagggtaaa ggcacacttt tagaacagtt agagtctgtc caatcaacaa
     6361 attggcatga gcttgcacaa gaagatttat tagccacctt tgaagaacgt caacaagaaa
     6421 cagatcaaga aattaatgag tatgcgacgg aacagtcgtc taattgggag aaagcaaaag
     6481 aggaactaaa taaagaagaa cagcgagtga ttgaagaaga agtacaaaaa attaaagagc
     6541 gatacgaaaa gaaacgcgac aatgcgtatt ccttgtgtgt agcaaagatc aataatttta
     6601 ctcagacgaa tcaagaagcg ttatcgaaag aaaaagaaaa cttgttaaaa gaacgtatta
     6661 aagaacgaaa agaagaattt taccgaggtt tgcgtacaga taagctaaag attagcaaag
     6721 aattaaatga acaactgaat aagctatatc aaacaacgac tgaagttctt atggaaaaat
     6781 acgaaaagat ccagcaagaa ttacaagtgc aaattccaaa atggaaaaaa gagtatgaaa
     6841 ttcaacaaca agaattgaaa gaacaagaag aaaaacaacg gcaaaaagaa aaagagcgcg
     6901 aacaattaga actaaaaaaa cgtgaacagg atcttgcgga acaacaaatg gcaatagaaa
     6961 aacaacggtt aaaccaagaa aaggaaaaag aagcagctgc tcgtaaggaa cgtcaagaac
     7021 aaatggatac attgcgacaa gctcagttaa attatctagc acaacctcaa ccaacgcctt
     7081 cagtagctgc tgttcaaacc ccaatgaaca accaaacaaa tctaactcct tcaccacagc
     7141 gttcctttaa cggatggatg gtgggatgta ttgcaagttt aagcttgctt ctaggtggag
     7201 gaacagtatt tgcttttaat catatcaatc aatcttcaca ggctgattct gtggccacaa
     7261 cgacgtcaat caaggaagaa gcccaagaac aagcaagaaa agagtatgaa caacaattag
     7321 aagaattatt gaatcgtacc aatcagtcaa caacagaatc aagtatggaa aatagcaaag
     7381 agagtgatcc tagtaaggct aaagaaggat caagcgattc aactgtacaa tcaaacactt
     7441 ccgaaagttc agaatcaaat gaggtgaaat aatgcgtgtt agagaaacaa cgagacgaaa
     7501 agacaaacga ttcaatttag aaaagaatag gtatctttct catttctatc gctatactcc
     7561 ttcaggagaa gagctaccag aaaaacatga caaacgtatt tctcaaaaga ttttttatag
     7621 tggcttagct tatcttcctt taaactatgt tgttacaaga ataccagaag catttcatcg
     7681 atttgagatt attccaggaa gtttacaagg agtgaatatt tttcagttac aagaaatgcc
     7741 gcagttattc cattttgatt caactggttt ttggaatcct ttgaccccaa caacaagttt
     7801 aggcacacgt ttagttgttt ctacgatttg tgttgttcca gctgtactta tgggattgaa
     7861 aatggacatg tggtttcgtc ctctagcaga tgggcaaaaa ggagataacc gattaaaaac
     7921 gttaaaggaa atccaacaaa tttatccgca aataccagaa tcaaagggag gttttcctgg
     7981 ttatggcgga ataccaatta ctcattataa aaagtactgg tatattcaga cggatacagt
     8041 aaatacttgt attgttggta cttcccgttc agggaaagga cagatagaag ttttagcaac
     8101 cattgacaat ttgtctcgtg cagaaagaca aagttcaatg gttgttaatg atccaaaaac
     8161 agaattatat gttgctagta aagatgaact agaagctaga gggtatgatg tgtatgcatt
     8221 taatatcacc gatccactgc aatcaatgag tgataatcca ttggcactca tcgtgaaata
     8281 ctggaagcga ggagacatag acacagcgac acagttaacc aatactttca cgaatacgat
     8341 ttattatgat gccaacgctt cagacaataa atactttaac gaaaatgcgc aaaaagctgt
     8401 caatgcgctt atttttgcgt tattggaaca agcggatcag acaggggatt actccaaatt
     8461 aacaccgaat aatctcgtcg aacttctttc tgagattggt ggttttaatt accaagatcc
     8521 tgataatgaa ttcaagcagc tcaatgcttt agatgaattt atgaatcagt taccacctgg
     8581 aaacatagca aaaaaacaat atggggcaac aaaaattggt tctgataaag cgaaaggcaa
     8641 cattctatcg acagcgatca caggactaaa tccatttaca ctgacgaaaa ttgcgaaaat
     8701 gacttctcaa tcaaccattg attataagtc cattggtttt ccgaagtatt tggatctcaa
     8761 actacacgag agtctgttaa accagcgaat tgttgtagag ttttatcatc aaggaaaaaa
     8821 atttcatgag gaacgtgtca aagtaggcta tagaggtttt tgtgaaatta actttgattg
     8881 tcatttagaa cagggagatc atgtttgctt aaaatacgat catcgaggaa aagaatatgt
     8941 tgcttggtat gagttttctc aaagagtatt gaaagacgaa gagggaaata tcgtttatcg
     9001 aaaaaaacga ggagaaaccc atttgccaga attagaaaaa gaagccgttc taacattaga
     9061 tgaggataaa agtaacttat tagtagagtc tattcggatg cactataccg ataaaccaat
     9121 ggctattttt atgctcacac ctgattatga tccatctaac catatcattg ttagtatttt
     9181 tcttagtcag ttgtataaag aattatctac gcaatgtgtt aagactaaag gagataaatg
     9241 tcaccgtagg attcatttta ttttagatga atttgggaat atgccaccaa tggataatat
     9301 ggaaggaatc atgacagtca cagctggt
//
LOCUS       DAJSMI010000102         9300 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJSMI010000102 DAJSMI010000000
VERSION     DAJSMI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.103"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..200)
                     /locus_tag="ON430_001945"
     CDS             complement(<1..200)
                     /locus_tag="ON430_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR3219857.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLL"
     gene            complement(522..1775)
                     /locus_tag="ON430_001946"
     CDS             complement(522..1775)
                     /locus_tag="ON430_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR3219858.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            1836..2576
                     /locus_tag="ON430_001947"
     CDS             1836..2576
                     /locus_tag="ON430_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3219859.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(2662..3420)
                     /locus_tag="ON430_001948"
     CDS             complement(2662..3420)
                     /locus_tag="ON430_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3219860.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(3417..4445)
                     /locus_tag="ON430_001949"
                     /pseudo
     CDS             complement(3417..4445)
                     /locus_tag="ON430_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(4465..7632)
                     /locus_tag="ON430_001950"
     CDS             complement(4465..7632)
                     /locus_tag="ON430_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219861.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(7856..8740)
                     /locus_tag="ON430_001951"
     CDS             complement(7856..8740)
                     /locus_tag="ON430_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR3219862.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(8835..9272)
                     /locus_tag="ON430_001952"
     CDS             complement(8835..9272)
                     /locus_tag="ON430_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219863.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT         2641 a         1949 c         1604 g         3106 t
ORIGIN      
        1 tgcagtagcg tgtcttcttc ccttgaggct ttttgttttt cgatacgttc ttcttcccaa
       61 acagacaacg ctactagaat ctctcttaat gattttcctt cttcggtgag cgagtactct
      121 actttaggcg ggacttggtt ataggctttt cgatggatac tttcatcttg ttctaactct
      181 cttaactgct gggtcagcat tttttttgtg atcgtaggga tttgcttgct tctccacgcc
      241 taaaataagc gatattgctc ttccctttat ctggtgtaag caaagcagtt agcattttga
      301 cgatcgtggg ttccgctttt cacttcttat ctacttagta tcatgaagta tactttcgta
      361 ctaatactaa gtagcggaag tgtcaactac tttgtttaac tacatagcaa aagaaacaga
      421 agggtgacaa cggtcatcac gcttctgttt cttttgaata tgctgtgttg ttgtctctat
      481 ctccgtgtag actttcctca aacttatcct ttgacagtaa gtcagtaggc tgatttgctc
      541 tttctagatg ccagatacat ttctacaaaa aaatagtgat tctgttttga aaattcgatt
      601 tttagttctg cattttcgta aacatccaac atttttttcg cactgtatag acctaacccg
      661 ccattttttt tagaggtttt tcctcttttg accatctccg atagcgtcaa attcgttgga
      721 tttgccttgt tcgctatact aaaacgtgat ccctcttttt cttccagcag attgatcgta
      781 atcggactct gatcggctac acgatgatcg aatgcgttag aaataaaaat agataaacag
      841 cgaataaaaa caaccagatc aatcgggatt gttttcactt cgacaggaat aactacttgg
      901 atcgggattt tctccgattc tgctttttct agaaatttta aaaagacacc cctgacagct
      961 ggggatacaa tatttgttag ctgttgtatt tgcggttttt caaaaatagc tgcagaatga
     1021 tcttctaata tttctaaaaa ttccaacgtt tcttttttat tttcctgaag agtaattttt
     1081 aatgaaaaca gtagactttt ataatcatgt ttgaattcct ctagatgatg gtaatattct
     1141 tcatttattt gatacagttg aagcgattcc tgatgttgtc tttcttttac ttggaaactg
     1201 atcaacataa tggaaaaaaa caaagaaaaa aaactgatga ctatcgacaa aagagataga
     1261 agcagataat tagatgagcg gaagtgaaca cttcccaata ccaagtaaat cacgccccag
     1321 cttaataaaa tacaaatacc tccaaaatag tgttcagttg gataagtaaa aagcaagttg
     1381 agaaatctcg tctgtttgtc tatacttttt actaatagat agaaaataaa tgcgataagt
     1441 agtgcaaaaa aacttgctgt tccaagatgt ctctttccta aataatccat cagatcaaaa
     1501 accagaaaaa agctgagtcc agtgatagca aaaaaaagat tactgatcaa acagctgata
     1561 aacaggtttt tcgtagtaaa ataaaaaaga aacatcaata ataagccaac taggtaagga
     1621 aagctcactt tagggatagg aagtaaaagt gtcaccacca tcaaagctat acaaccaaag
     1681 ctgtttttta ctagcagttg tttcgaaaag aataaataga tacctaaatc taaaatcaaa
     1741 gtaaaggtca aataaagtgt cactgttgcg accatcttat tcctccattg ttttgtttca
     1801 aaatgtacac atattttttt aataggagcc tgcatatgaa aaagtatttt atcatagaag
     1861 ataaccagtt tcacagcgaa tggatcatca gagaattggt caatcatcat atcgagaata
     1921 tcgtgcagct cacttctatc tctcaaatca atcagttgat ccagttgggc actattgata
     1981 atcacgatgt tttctttatc gatatcgact tgaagcaatc actcacaggt attgatgtcg
     2041 ccgaacaaat acgtaaaaaa aatgcacata gtaatattgt ttttttcacc gcttatacga
     2101 attttgcagt agagatcatc aatcggaaaa tccttccgat tggatatatc agaaaagaca
     2161 ggaatttatc tgaacaaatt cgtcaagtgc tagaacagat caattggaaa gaacagcaga
     2221 tcgtaaatgt cgataaaatc tttattgatc aaaaaggtgg aagtctcgtc ctgtatcctc
     2281 gggaaatttt atatattgaa agtatcaaag gtttgaaaaa ccaagttatg gtcaaaacaa
     2341 ctacagaaga taaattagtg aatacttctt taaaacaatt gaagacagag ttagaaaagt
     2401 atgcttactt tttactttta aaatcttact gtatcaactt atcgcaattg cagaaaattg
     2461 attctgctca ttatgtgtta gaatttttca acggtgactc cttactcgtt ggtcgaaaga
     2521 tctttgaaaa gacaaaggaa cgtttccatg acttccaaaa aactgcttcg agttgatgaa
     2581 taagagctga gacgtaagca tagcagattt cagggagtgg gccttgaatc ggctccactc
     2641 cctgaaatcg ttgatatgat tttacgcttc ctcttctgta ggagcatctg ttaacgacca
     2701 tgtgaatgtt gaaacgtagt tgtctacagc ataattggat acaggaacaa ataattccac
     2761 tgattgtctc ccttgttcca cattctctcc taatgaataa atccatgtgc caatcccttc
     2821 atttgctcct gcagttacta gatctactgc ctgatctgct gggatttcga ttccttcagc
     2881 gccagttcca gaaacggtag atggcattcc actgctatta gttgcgctat ttcgaatcac
     2941 ggcgtttttg aatttcacct ttgaaccagt tagtttattc gtatcagatg ttgtaccaat
     3001 aaattctgtt cgtttgactg ataatttcca accggcataa tttccgcttt tatccgttac
     3061 ttgtacgaaa ttctgtactt gtgtttgtgc tccagtactg tccgttccgc tttgtaatgc
     3121 agcatatact gtttttgctg tacctaactt gattgttcca aaatcaagat ctggagcttg
     3181 gtcaatcgtc agtaatccgg atgttccctg atttggttcg tcaggtgtaa aaggagtatc
     3241 aggatcattt ggatcaacag gaggaactgg aacaacgtct tcaggatttg cagcatctgg
     3301 tttggtcaca gaaatcgttc cttctgtagt ggctgagctc ttattctcag caaaaacact
     3361 ttgtgtacat aataatagac ttccagctag aacggtactt aatgcaattg tttttttcat
     3421 ttctttttcc ttctttcttt taaaataaat aatccagcaa ataagccaat aagaatgact
     3481 ccaacaagta tcagccatcg ccagtcaaat gtttctggct gattttttaa atcaatatcc
     3541 ttttcattca attggtccgc tgcatcatca gttatttcaa aatcggtatg gagattccag
     3601 cgtttttctt tccaagcacc cttcacaacc gcttgataat gtcccggagc taattttttt
     3661 ccttctaatg ggacaagtaa atcaaaattt gtattgggtg ctacatctat tttttctttt
     3721 tttattgaaa ggtaaggctc ttttgtttct tttttgtaga tttcaaccga tgtagtcaag
     3781 ccgaacatat aagaagcatt tggattttga agattcgcct taatcacatt tcttccatcg
     3841 ctttgatccg cataagcttt ttttaatttc aaatctgggg aagactcttt ttcgctattt
     3901 caaatcaaga ttgctttcac ttgagaaaac tgattgacga caccaactgt acttttgtta
     3961 gaaacgttct cttttttttc ttgaaacgtg atccctcctg cgaccacacc agccagctct
     4021 ttttctggcg ctttcagtga aagtgtctca gtgaccgcac tttgaggagg aacagttatt
     4081 tctcccactg tttttgctaa gtttttcata tcataactta atgtttcatc aaatttactt
     4141 tttgattctc catattcgat cacaccgttt gaattcgtcg tagcggagtg gatcaatgtc
     4201 tcaactgtta ctgatttatc tgttcggtta aagaccgtgt aggggatttc ctgtgttttc
     4261 ccaggctcca tccttacatt tatatatgtt ttgttcttat caatctgatt agccggtaat
     4321 tcaaaatcta ccgcaaaatt gaattcggag gcatggacag gtgtctgcca aaggatgaat
     4381 ccaaggagaa ggatcataag taaagagcag ttgtttcgat gacttttatt ccatattcga
     4441 ttcatcaatc atcctccttg atctttattc tgtcggcgca tctgctaatg tccatgttat
     4501 ctctgctgag taattaccag ctatctgatt ttctttggtt aagacaagtt taaacgcccg
     4561 accgttattt aaatattctg aataatctgc ttgcacattc gttgaatttt gtcctctgac
     4621 aatttccagt tcttcttcat tcactaattg atactcctct cctttcttga aatataggac
     4681 atcctgcata gaaaatcctg catcatttgt gaaaggctga gataatttta tcgttaagat
     4741 ccacggattt ttcgtttctc gctgatcata aatccataat ttttcctgta tatccatttg
     4801 gccaaatgct tcttgtgtca aaccagttgc tttgatctga ccaaaatcga gtttggaagg
     4861 ggcaagaaat gagagaccac cttctactac gatcttcgta gcaggtgcaa aaggcggggc
     4921 atcatgataa acaactggcg tatcacttgg attgtgattc ccaatttcat cgttggtact
     4981 tggcttgttc tgcacaccct tagcagtagc ttctcctgct tggtcgttta atacaatttc
     5041 taatacatct cctgcaaaaa gttgattttc tgggatagaa agaacaaatt cccctgtatc
     5101 atcaatagtt gtttctgaca aaacagcttg attttttttc aatgaaacca aggctcctgc
     5161 ttcacctgta cctgtgatcg tgcgagaagc tgcattgatc aatgtggtac ttaaattagc
     5221 aggttcagga ggaatcacat cgactgtcgt tttggcagag tccagttctg tcacttcttg
     5281 gccacctctt acttttttag cagcaacaac ttgcacttga tcctccgttt ttaatctctc
     5341 atcattgggt acttgaactt cgaacaagcc gccttgttcg ccttctcccc atgggttata
     5401 tcctgcccct tcattttcat ctgtttgtcc ttgcgtgaga gcagtaattt ctacagatga
     5461 tttcgtcgta tctgcgtaag taatacgtaa agtcacttct acttcgcctg tccaagcatc
     5521 tcttggcttt gcctcgacac cttcgggaac aacgacatgc ccatagattt ttttatctgc
     5581 attcgttggc actcttaagg attcaatcat cgggagctga ttattggcac tgattcgatt
     5641 ataatttttc attccggcac ctgtctgagc aaattcattt gttaaacctg cactagggct
     5701 tgatacgaaa ttgcctaagt cggtcccatt gatcgataag tctgtcatat cagaaaaata
     5761 atccgggtct ttttcaaagt tatcgaatgt tgcgcctgtt tttcgccata aagcgatttc
     5821 tgaatgggcg atctttaaag aagaatctcc atttccttgg aatacccagc cgcctatgcc
     5881 attggtctcg cttccaatcc gtttgttttg gaaatcaaaa taggtaggag attctaagtc
     5941 cacattcaac acatccccac tgatgatgcc ggtcttatct tcccctgttt tcccgaccat
     6001 gactaaataa gttttttcac ctgccgtaaa atttaaatca atatctccag ttgtatctac
     6061 agttgctccg gaatctgcgc gtatatcgat atttgatttt tctccagtga ctgtaaaatc
     6121 agctgaacct ggactttcta gaaaaccaga agcgtcataa aattggatcg cttgatttcc
     6181 ttgattgagt ctgttcacgt aagatttgcc atcacctgta tttcttaccc tcaatgagcc
     6241 tccattggtt actgtcagct tttgcgtacc attctcaaaa cgaactgcgc tggaacgccc
     6301 actctgctta tcgatgtcga taactgatcc ggctccttct acgatcaaag aaaggccttt
     6361 tcctacaaaa cgcatcccat tgttcccggg ttgatcgtca tctccagcca ttttgaccga
     6421 gatttgtcca ccacttgata catggatttg actagaatct ccttgcaagc gaaatccgcc
     6481 cgtagttgct gtattgatgg tgattttccc accattcgat acagtgactt tcgatcctga
     6541 acctctcatt tggatgattc cgcgattgtt gtcattggta atattgatct ttgtatttac
     6601 cgaaaattcg cttcccgaac cactgacttc aaacatgaca ttaccaggta gcgtatcgac
     6661 tgcctctcct tcatcgctaa cgccatctgc ccgatcataa tcaagagaga cctttgaacc
     6721 gcttaagata tggattcctg gcgctgtttt tccactacca gtctcatctc ctgaatctag
     6781 aaaaccgtag aatttcccta gatacggtcc ataaagttga ctaccattga tcacattcaa
     6841 ttgatagctt ctacaaaaat aggctttacc cggagcggca ttcagtcctt ccttcactgg
     6901 ttggacatcc aacacaccgg aaacaccatc taatgtgaca gttgcacgag gagcgtaaat
     6961 cagtcctaat ttggacccat catacaaatc aagtgtcccg gtaaatttta tttctcccgt
     7021 aaaatgatat ctgcttcccc aaaaatcggt catataagca atgataatgg catttccatc
     7081 gatatttggt gtcaatccac ctggatgtgc agctgttcct ttaaaattct ggaactcgat
     7141 cgactgaggt atctcattga tataaatatg gtgtgtgccg aaattgtgag cgaatccacc
     7201 aaaatcaata acgatatctc gtttgatctc taattccctg tctaaagtca aaggctgaat
     7261 cagtcggatc ttagttactg attcgtctgc gagaagctgc gccagttctt ctgttgtggc
     7321 agcttcgctg tacccatccg ctgaaacagc tcttgccagt gccgcttcac tgctatcaga
     7381 cgtttgttca gctacttctg aggtctcact tgtcttattt tcttcttgcc ttttattagt
     7441 agaagcctca ctttgagatt ctgtagtttc agaagtaatc gattcttctg aaaaagtagt
     7501 cttgtcaaaa ctttgattct ctgattgcgt tgattcgttt gttttctcta catcggcaaa
     7561 aacggatgtc accatcaaga gagggaagac aaacaaaatc gacgcaatca gcttttttac
     7621 acattgattc attctccacc atcctctctg acgttttgac aattttattt tatcgtttta
     7681 atctatttcc aattgtactt tcatatttaa aatatgtatt tttatggaaa aatttttcta
     7741 ttaggtgtaa atttcttttt tgtacaaaaa aaaggctgtg acaaaagtct tgtcacagtc
     7801 ccttatcccg aacaagcggt gttctcccgc caactcctcg gtcacaacct ccattttaac
     7861 aataaacctg taaatgtgag ggcaatactt tgattgtcac aggtaattta tccccttcat
     7921 ctccatcaac attcgtcgtc aattcttcct catcagctaa tgacacaact aattgctcac
     7981 aagtaaggta ggctagattt ttcgtagatt catctactcc ttttaatagg tctggcaatg
     8041 ttttaagcgt atcgatcaaa gaagaatctt ttaaataaac caaatggagc ttaccatcat
     8101 ccacttttgc atttggaagt aatgtttcaa aaccgccgac tgagttagtt agtcctatca
     8161 gcaaggtact gctctcaatt tcttccttct ttccatcaac ttctacgtga aacgagtagg
     8221 attgtgtgga agctaactgt ttgattcctg acatgaaata agctagtttt ccaaatttcg
     8281 tcttcttctc cgcatctaca tcattgattg attcagggat agagccaatt gcgacgacat
     8341 tcataaaata gtcgtcattt atttttccaa tatccaatgg cttgactgat tcaatcgaaa
     8401 aatgattgat cgcttcctca ggttctagcg gtatccctaa tgctcgagcc aagtcattca
     8461 ccgtacccaa tgggaaaaat ccgaaatttg gccgatgttc ttgttcagcg attccactga
     8521 ttccttcgtt gactgttcca tctccgccca tgacaaacac actatgatac ccttccgtag
     8581 ccgcttcgcg agtaaagttt tttgcatccc ctgctttttt tgtatgcaag acgaccactt
     8641 catcaaacac cgattccaat tttgctatcg ctaattgctc aaattccttt gcttgttcac
     8701 cgcctgaact ggggtttacc actaataaaa cttttttcat cactttcttt cccttctgat
     8761 tgatttcttt aatataacga tagcataaag aaaaaaatta ggctgtgatg aagtactgtt
     8821 catgaaacat tcatttattt tttgacaaga taaaacatcc atactatgaa actgaggaaa
     8881 attaaaacaa tgatatagga aataaaagaa aatgataggc attgtatacc taataaagta
     8941 aaaatcttag gtggcagata aaaacacaaa aatagcaaga gaatgtaata gcgtttcttc
     9001 cactttttgt tatcagacct tatagcgatc agaggaatac caaatgcata actgaaaaac
     9061 tcttgatgta ctgttaatat gtcacggcct tgaataataa atgcgacaat ccccgctata
     9121 attagtgtgc ctaacaccaa aacggaaaat tgatgaatat attttatata caaatgatcc
     9181 attgtacttc ctgccttttt atttgtgaga aaaacaaatc cgtatacgtg aataacttct
     9241 agaaataaaa gaaatttgca aaaccactcc atttatctac ttctacgtgc attttttgat
//
LOCUS       DAJSMI010000103         9151 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJSMI010000103 DAJSMI010000000
VERSION     DAJSMI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.104"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..206
                     /locus_tag="ON430_001953"
     CDS             <1..206
                     /locus_tag="ON430_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219864.1"
                     /translation="KSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGW
                     TMNIGIYYTYNPVAGTYYVVQNI"
     gene            331..2682
                     /locus_tag="ON430_001954"
     CDS             331..2682
                     /locus_tag="ON430_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCR3219865.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            2762..3157
                     /locus_tag="ON430_001955"
     CDS             2762..3157
                     /locus_tag="ON430_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219866.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3482..4099
                     /locus_tag="ON430_001956"
     CDS             3482..4099
                     /locus_tag="ON430_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3219867.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            4140..4439
                     /locus_tag="ON430_001957"
     CDS             4140..4439
                     /locus_tag="ON430_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCR3219868.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            4473..4640
                     /locus_tag="ON430_001958"
     CDS             4473..4640
                     /locus_tag="ON430_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3219869.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(4685..5290)
                     /locus_tag="ON430_001959"
     CDS             complement(4685..5290)
                     /locus_tag="ON430_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3219870.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            5415..8402
                     /locus_tag="ON430_001960"
     CDS             5415..8402
                     /locus_tag="ON430_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR3219871.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            8551..8760
                     /locus_tag="ON430_001961"
                     /pseudo
     CDS             8551..8760
                     /locus_tag="ON430_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8866..>9151
                     /locus_tag="ON430_001962"
     CDS             8866..>9151
                     /locus_tag="ON430_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCR3219872.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDA"
BASE COUNT         3126 a         1397 c         1741 g         2887 t
ORIGIN      
        1 ggaaatccta cattgacgat ggaagagtag gaaatgtaaa tcaagccaac gcaactaaat
       61 ctggttctat tcaaggaatg gcattgtatg ctcgtgaagg attcaaatac tttgcaccaa
      121 acgggcctgg ttggacaatg aatataggta tttattacac ttataatcct gttgcaggta
      181 cgtattatgt tgttcaaaat atttaataaa acagcacaaa gagatttctt tgtgctgttt
      241 tattattttt agttgttgac aaacactaaa atataaaata taatcaacac ataaacgaac
      301 gtatgttcgg caagaaagca ggggattttt atggatgaga tggttttaag tactcagaaa
      361 tggctaaaca agaaatacag taatgttaca gggtttgata aggttccaga aaatgggcga
      421 acagggtggc ctacaatcta tggacttatt gaaggactac aagtagaact tggtattact
      481 aatctagtag ctaattttgg acctaccact gaaaaaatgt acgataatca ggttactcct
      541 aaatggggca aaaatctacc taaaaatatt gtatttctta ttcaaggtgc cttttggtgt
      601 aaggggatta atccaggtgg cttcgatggg gtatatactc catatttaga tactgctgta
      661 aaagagttgc agactgatgc agggtttaat ggaagcgctg taactggagt tttggatgcg
      721 aaatgggcca aagcgttatt tgatatgagt gcttttgttc ttgttcccgg cggagatagt
      781 aaaattagaa aaatgcagca atggttaaat gtaaattatt tagaatacac gggaataatg
      841 ccttgtgatg gtatttatca aagaaattca aatcaagcat taatttttgc attacaagct
      901 gaactaggat attctcccag tgaagcaaca gggtcttatg gtaatgggac aactagtaaa
      961 acttatcctg tgtctgaagg taactcggga aactatgtaa gaataattca atatggatta
     1021 tatgttaatg gttattttga agacggtaca ttcgatggta ttttcactaa atatatgggt
     1081 ttagaagtac tggcattccg aaaatttatg gtattgcctg aatatactga aatagcagat
     1141 gttgttgtaa taaaaggact attgtctagc gctggagata ttagaagatc tgcagatggc
     1201 gcagatactt ctacgcaatt aactagatct caaatccaaa cattggttga taatgatata
     1261 aaaatagttg gtagatatct tactggtact gttggaattg gaaaagatga acgaaataag
     1321 tatctaacta cagaggaatt gaataatatt ttttctaaaa atttatctgt gtttcctatc
     1381 tatcaagatg gaggtgcagc attagcttat tttacttatg atcgtggctt atcagatggt
     1441 aagaaggcta tagatgcagc aaaaaatcta aatataccat taacaacagt tatttatttt
     1501 gctgttgatc tagacatgtt aggggaagaa attcttgcat atgctggaga atattttaag
     1561 ggggtatctg ctgtaatgtc atatagcggg tatcaaacgg gtgtttacgg aacaagaaat
     1621 gtttgttctc aaataatcaa taacgggtat gcttcgtttg catttgtttc agatatgtcg
     1681 actggttata gtgggaattt aggttttcct atgcctagac aatggtcttt tgatcaattt
     1741 gttgaattta ccataggaag tggcaacgga gctgttggta tcgatttaat cgctacatcg
     1801 ggtagagata gcggatttaa tgagttatca aatgatacta ataatgataa ttacattgcc
     1861 aaatacaatc agaaggttat aaatcagatg gtacgtgcgt atcaatatct atcacaagct
     1921 ggtcttgata atccttggaa ccctcatttg acattttata gatatgtaaa ttattctggt
     1981 ttgtcatggg acatcatttc ttctcctgta acagagcatg atagaaaaat ttatgatgaa
     2041 tataggaata aattaactaa tagcgagggc ctgtataatt attttataga tcctaatact
     2101 ggttcaataa taggtttgcc tcacttaatt gtgacgttac aatcacagat gtttctagcc
     2161 gacacaatca atgatagtgt ttctgacttt gcaggttggt taggagattt aatgacttgc
     2221 tggggtgaag ttaagaaatt aggaatacaa atggagcaag gtgtatttgc attagtaggt
     2281 acggctgcag gtggaacatt tagtctagaa gatttatacc aagatgttga cgcggtaaat
     2341 gtttatacct tagcaaaaag aaattctgat tcaacagctt tatcggcttt aaaaacgtac
     2401 tattgtgata agggtgctat tgatagattt aatcaatttg ttaagaacag atttggttct
     2461 tcagataaaa tcgcttcagt aacaaatagt ttattagaaa atgacgaaac agttgatatc
     2521 ttaggtgtag ggataatttc atttttcatt gataaaatag ttaataattc acttgggaca
     2581 gattttactt atcttgattt tattggagat gaggggaaag ttgcaaatgg attttctgaa
     2641 aaaattattc aatttcaaga aaatagaagt aaacttaatt aagaatcgtt tactatttaa
     2701 acagtgtcct tttccttttt aaatatatac gataaaataa gtatatgaat tgtaaaagga
     2761 catgatgatt atggaagaaa tagaatggaa taatctgact caaaataata aaaataattc
     2821 tggatgtgga attatcttta tgtttgttat agttttggga atagtagtac ttttaataac
     2881 tgtcaagata gtagattact ttgcgaatac tcctgaagaa caaatacaga gaaaacttcc
     2941 tgagtttgaa agagtaccac cttatgatcc aagagataat tcatttcaag taaaaaaatt
     3001 aaaagagtct ccaaatgaaa tgtggtttga agttacagtt gaatcaagcg acttcaaaga
     3061 aaaaaagaaa gaatacaatt atattgtaaa ggcaaaagta tcaaaaagat ttatttttaa
     3121 ccctaaatta gaaatatata gtgttgtaat agaataatag tgttaaaaaa attgtaaaag
     3181 ttaaagaagt caaattctga tgtgaaaaat aaaatatacc atggaagaca taaaagaaaa
     3241 actggataac tagtttattt aatggcagac cgttaaggcc tgccattttt atgtatttta
     3301 gcggaataaa tataatcatg cgggagtgta aacttactgt ttcaaaaagt atactttttg
     3361 acatgccttg attttctttg tttatgtatg gcataataac cacataatac aatttgaaac
     3421 gtttctgatt taatctgatt gttttgaaac aattttagtg aaaaaagggt agataaaaac
     3481 gatgagaaaa tttttttata tccgggtcag tagttatgat caatcgatcg aacgacaact
     3541 gatttccgcg aaggaattag gaatccctga agaatatata tttgtagaaa aagccagcgg
     3601 caaagatttt aaacgccctg aatatcaatt aatgaaacgg atgtttcgaa aaggagattt
     3661 actctatcta caatcattag atagactagg cagaaataaa caaatgattt tagaagaatg
     3721 gcgagaatta actaaggaaa agcagattga tatttctgtg ttagacatgc ctctgttaga
     3781 tactgctagt tacaagcaac tagatgggtt agagacgctt atctctgact taattctgca
     3841 attactcagc tatatggccg aagacgagcg gaaacgaatt aatagccggc aaagagaagg
     3901 cgtagaagct gcgaaacaaa agggtgtaaa attcggcaga aagaaaattg ctttacctcc
     3961 tgaatttcct gcgatctatt ctgactggaa agcaggaaag ataactgcag tctatgctat
     4021 ggatcaattg ggcatgaaac gaaatacctt ttatcgacgg gtgaaagaat atgaacatac
     4081 cttgcaccac ttttcttgat agatataaca gctattagac gggaagggag agtttgaaaa
     4141 tggacttaac gaatcaaagg gtcgttttta ctggaacgct gcagcaaatg acacgaaccc
     4201 aagccatcgg tttggttcac tctttaaacg gccattgtca atcaagtgtt acttcaaaaa
     4261 caaatttcct ggtttgtggt cagtattcta agtcgttgtt tgatgattct ctctatacaa
     4321 aaaaggaaca aaccgcgcgg gacttgattg cgttaggtca tgagattacc attttatcag
     4381 aagtcgaatt ttatgcgtta attagtcaac agttgaaaga atggcaacgg tatttataat
     4441 cgttagtaaa atttacaagg aggaaaattt ttatgaagcg aaaatcttat gatcgttggg
     4501 gcattgaaat aactagtttt aatcgaaata ttgcttttca cgtggagtgt gactgtgggg
     4561 aattagcaaa aagtgtttat caaaaaaatt acttttactg tcctaaatgc catcgccgat
     4621 acggggtaca tggaacatag aaacgaaata gtgcggtaga aaagcgaatg aatgtagtat
     4681 atttctacaa atcattcgct ttctttattt tataaatagt tggtcgagag atcccgtttt
     4741 cttttgcaat ctctgcaact ggaatgcctt gctttaacat gtccaccacg gttttaaaaa
     4801 ctaagcgttt ttgcggatct gttgagttaa tagaatactc gattggtcgt cccttatatc
     4861 ggccggcttt ttgagccaat aatactcctt gccgctgacg ttcacgaatt ttctcacgct
     4921 cgttttgtgc aatataactt agcagtgata aaaacatatc gaacatagcg gtatccagga
     4981 gttcatttcc agtattaaaa tctaaaaact gtgcatctaa tatttttaac gctacttttt
     5041 tctgtttcat aaaagcaact gtatttttta tatcttcata atctcttcct aaacgatcta
     5101 gtgatgtaac gatgacgcaa tcaccttcac ggatataatt tagtaatttt tgaaactcag
     5161 gacgctcagt atctttacca ctggcttttt cttggaataa tttatcaatg ttaagtcctt
     5221 ccatcattgc caattgtcga tccaaatttt gatcttgtga agatactcga atatagccaa
     5281 tttttcccat aaaaaactcc tttttaaaag tgatataatt tacatgtaaa atttacttgt
     5341 agttttactg ctatcatagc atttttaaaa ataaatttta tgtagactta aaatttactt
     5401 catggagggt ttaaatggat gaaatcaaaa gtaacaaaaa agcatttggc atttatttag
     5461 gagaacctag tacagaacaa ttaaaccaat ttttttattt gcatgaaaaa gatttagaat
     5521 tgatggagac tatacgacga ccagctacta gattaggttt tgcactacag ttagtctcac
     5581 tacgtttttt aggaactttt atattagatc ttaccaatat tccccaaaat gtattgagtt
     5641 atgtagcaac tcaactaaat attgatcctg gagaaattat ctactatagc aggagacaga
     5701 ccaggtatca gcacacaaaa ttgataaaat cacattatgg atatcaagat ttcaaccaac
     5761 cggaaataga aacataccta gctaactggc ttttaaatag agcgctgtat acaacagaat
     5821 cttcagatat gttgtttgat atgcttctaa aaaaatgtct ggatgaaaag attgttttac
     5881 ctggaattac tactttttct cgttttgttg ctagtgcaat tgaaaaagct gaagagcatt
     5941 tataccgaca attgtctttg gttccaactg aagatgagag aaagcggctt ctcaatcatt
     6001 tagaattaat tggaactcca gtttatgggg caataatcaa aatggacatt ttgagaaccc
     6061 cgttagctga cgatagtcat aaagaaatag ccagaggttt tgaccgacta aagcaatttc
     6121 aagctttctc tactgaaaaa tggaagatgg aatctattcc tgaagggaaa ataaaagttc
     6181 ttgcaaatta tgcatttaaa gctaaagctc aattaattca acgaatgagt gaacagaaaa
     6241 aactggcgtt gttagtggca tttattttca tatacaaacg taaggctatg gatgaacaaa
     6301 ttttagcact atctaatttt tttgaaactg tttttagacg cgccaaaaat aaggaatcta
     6361 aagaacgtct tcgtaccata aaagatctag atcgagccgc atcgacactt tcagatattg
     6421 tagatttagt aatgtccagt cattctgata ctaatgaact atcccttcgc gatcgtatct
     6481 tagaaaaata ttctgaggaa aatgttaccg aagctgtatc gcaagttcgg ctattggtta
     6541 aaaatgatcg tgaaccaata gccatttctg aacttctgaa cacttatcga aagttcagaa
     6601 aatttattcc ggatatttta actacactaa catttgacag caatgactac ggtaacaact
     6661 gtaaggaact ctggaccttc attaataata gatttcctaa gccgattact tacaaaatgt
     6721 ttggagctat taaacatact cttccaaaga aatggattta ttacattcat gaataccctg
     6781 aacaaactaa taaatgtgta ttgattgcag ctgtcgaatt gttaattcaa tctttaaaac
     6841 gccatgatat ttatattcct cagagtgtct tatataacaa tcctatggat tgcttgattg
     6901 atgagaaaac ttgggttcaa caaaaatacg ttttattaga acaactagat cttccttctt
     6961 cagcaaatga aatgtcttta tggttaaaga acgatttgga actgtcttat aaagaagcgc
     7021 taaatcgttg gcctacttct aaaatggcca gaatagaaaa tgcagatcgc ctggttgtct
     7081 ctagacttaa aaagacatat gaaaaaaagg aagataagtc ttttagacgt cgcattcagc
     7141 agttacttcc taaaattgat ttgtcagatt tgttattgga agtaaaccaa cagttaaatc
     7201 ttactcaatc atttaaccac ttaagtgaca aggacaccaa aatgaaagac ttggatatta
     7261 gtttattagc agttctttta tcagagtcct gcaatattgg tttttccccg gtttcaaaag
     7321 aaggatttga ttctttaaag tatgatcgtc ttgtctatgt caatcatcat tatattcgag
     7381 tggatacact ttcaggagct aatcaaaaaa taatacatgc acacaggaaa cttgccacct
     7441 ctaagatatg gggtgatggg catatggctt cagctgacgg tattcgttat atcacaccac
     7501 aacgttccct atattctaga agtaatccaa aatattttgg ccgtggtcgt gggatcacat
     7561 tctacaattt tgtctctgat caatatatag ggttccacgg aatggtcgtt tcagggacac
     7621 tgcgagattc gctttacctt ctagaaggat tcctcaatca acccagcgaa ttaactccta
     7681 atcagatcat gactgataca gctggttata gcgatatgat ttttggtctc tttggcttat
     7741 tagggtttca atttagtcca agaattgcca atgataaagg aaccaaactt tggcgaatcg
     7801 acgctaacgc tcactacggg gaacttgata cgttgtctca aaattcaatt aatatggaac
     7861 tgatttatac ccattgggag gacattctta gggttgcagg ttcgttgaaa tctggaaaag
     7921 tgaatgcaac agaattaacg aaatctcttc aacggaatgg gcaacccacg cctttaggaa
     7981 aagcaataac tgagtatgga aaagtttata agacgaaaca tcagcttcgt tatatttctg
     8041 acgaaatata tgcacggcaa attttagaac aattgaataa gggggaagct cggcatgcct
     8101 tatgccgtaa tattttttat ggacgaaaag gaaaactcta ccagacttat tatgatggga
     8161 tggaagagca gctcaatgca ttaggattag ttactaatgc catcatatat tggaactcac
     8221 tgtatttaga aaaagtattt gaacaaatga aagctgaagg attcgactgt tccgaagaaa
     8281 tgatacgcag gcaatctcca ctaattagtg atcacatcaa ttttgttggt aagtatacgt
     8341 ttagatacga agctgaatta tccaacggta agttacgtcc attgaatgta gaagaattct
     8401 agcaatgttc gggttttgca tgctaccgca ctatttcgta tgactgttcc agcgaatcac
     8461 ttcgaataga aaaagagaaa aacacgagaa cagcgttaaa tcaacgtttc ttgtgttttt
     8521 acttttgtgc taccgcacta tttcgtttct atgttccatg taccccttta tcaagaagcg
     8581 caaacacgcc aaattgccga ttggttgatc ggcatgaacg caagcccttt atataccttg
     8641 aatttacaac aaaaaggcgt acaaggtaca ttttcattag gacgtgtaca aacaccgact
     8701 ttatatctca tttatcaacg ccaggaagca atagaaaact ttaaaaaaga accttttttc
     8761 ctgaataatt catagtttta ctaatagtct tggttctgtt gcaaagtttt aaatctacta
     8821 tcaaataagg tagaataata gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa
     8881 ggaaagcaat ttcagcagga tgtgattatt gtagccgtgg gctactatct tcgttataac
     8941 cttagctatc gtgaagttca agaaatctta tatgatcgtg gcattaacgt ttctcatacg
     9001 acgatttatc gttgggtgca agaatatggc aaactactct atcaaatttg gaaaaagaaa
     9061 aataaaaaat ccttttattc atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa
     9121 tggcattatt tgtatcgagc catcgatgca g
//
LOCUS       DAJSMI010000104         9098 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJSMI010000104 DAJSMI010000000
VERSION     DAJSMI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.105"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            202..426
                     /gene="nrdH"
                     /locus_tag="ON430_001963"
     CDS             202..426
                     /gene="nrdH"
                     /locus_tag="ON430_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR3219873.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            585..2744
                     /gene="nrdE"
                     /locus_tag="ON430_001964"
     CDS             585..2744
                     /gene="nrdE"
                     /locus_tag="ON430_001964"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR3219874.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2794..3759
                     /gene="nrdF"
                     /locus_tag="ON430_001965"
     CDS             2794..3759
                     /gene="nrdF"
                     /locus_tag="ON430_001965"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR3219875.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3848..4987)
                     /locus_tag="ON430_001966"
     CDS             complement(3848..4987)
                     /locus_tag="ON430_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3219876.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5296..6009)
                     /locus_tag="ON430_001967"
     CDS             complement(5296..6009)
                     /locus_tag="ON430_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR3219877.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6263..6964)
                     /gene="nagB"
                     /locus_tag="ON430_001968"
     CDS             complement(6263..6964)
                     /gene="nagB"
                     /locus_tag="ON430_001968"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR3219878.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7198..8730
                     /locus_tag="ON430_001969"
     CDS             7198..8730
                     /locus_tag="ON430_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3219879.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8921..>9098)
                     /locus_tag="ON430_001970"
     CDS             complement(8921..>9098)
                     /locus_tag="ON430_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3219880.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         3029 a         1774 c         1591 g         2704 t
ORIGIN      
        1 tagttttcca caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc
       61 agctcttttt tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag
      121 tgtttgttgt ttgacttttt tactaagagt tttatactat ctattataga taataagaat
      181 gtgttagagg aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa
      241 tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc
      301 gatgaacagc ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc
      361 attacttctg atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc
      421 agctaacatg actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca
      481 ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt
      541 gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt
      601 tacaagatgt cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa
      661 tccctttgaa taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa
      721 atacgatgac ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata
      781 tagaagcaga attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt
      841 tggacgagca acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat
      901 atgcgttgaa aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct
      961 tcaatgcgct ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa
     1021 tgatccatca acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc
     1081 ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc
     1141 gctcaatcaa ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat
     1201 caaatcttcg tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg
     1261 ttccagtcat gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc
     1321 aaggagctgg cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag
     1381 caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg
     1441 tacctgataa attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat
     1501 atagtgtcga aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg
     1561 atcatctagt tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa
     1621 atgaaatctc aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag
     1681 cgaaccgcga gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt
     1741 tacaagttca aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag
     1801 atatctcatg taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg
     1861 gtcaatctgt gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg
     1921 atgtcgtacc accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca
     1981 tgggcttaca cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag
     2041 actttacaaa tatttacttc atgctgttga attattggac acttgttgaa agcaacaata
     2101 tcgctcgtga aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca
     2161 cttatttcga aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat
     2221 tgtttgaagg catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa
     2281 tgaaagatgg actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat
     2341 atatcaacga tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag
     2401 agaagaaaat cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt
     2461 attacacttc tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac
     2521 aacaacacgt cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag
     2581 gcatttatga atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac
     2641 attacgcttt ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg
     2701 cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc
     2761 gcttattcat attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata
     2821 aactggaatg aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa
     2881 ttttggctag atacacgtat cccactttca aatgacctag atgattggcg tacattgtct
     2941 caattggaaa aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc
     3001 caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc
     3061 gttttgaaca atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt
     3121 agtacactga atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct
     3181 tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa
     3241 aagaaaattg ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct
     3301 ctttattatt taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc
     3361 cgagatgaat ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag
     3421 ttacctgaag cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt
     3481 tacgaaaacg aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa
     3541 gtcaagacat tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca
     3601 ctctttccag atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc
     3661 acaagcaacc atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa
     3721 gcgatgaaag acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa
     3781 tttttcgaaa aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt
     3841 tttatgctta ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat
     3901 gttccattct tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg
     3961 catagacagg tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca
     4021 ataccaagat gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga
     4081 tcaaatttcc atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa
     4141 atggagagat aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat
     4201 acgggataat attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga
     4261 atattctcat tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc
     4321 tgatataggc agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa
     4381 tcgctttcgg aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa
     4441 atagcaaaat aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga
     4501 tttttgagac acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata
     4561 aatttgctga tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca
     4621 attcatttaa ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa
     4681 ttgtctgttc cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta
     4741 cgaccaaaaa cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa
     4801 caacgattgg atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg
     4861 ccgaaagcat tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc
     4921 cgatgatcgt gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg
     4981 attgcataca tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt
     5041 acgcgctgag attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt
     5101 agacgagtac ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc
     5161 tttttccaaa taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa
     5221 aaatataagg aactattttt atttggctgg gcttattact tgatgtagga catctatttt
     5281 acaacctcag atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg
     5341 tacggacaac tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata
     5401 gatatttttg tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat
     5461 tacgatattt ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat
     5521 aaattggatt tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag
     5581 tgcaatagca atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc
     5641 gatccctaat ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg
     5701 tttcgtgacg atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat
     5761 cagccttgct tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct
     5821 taatgcacca ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac
     5881 taaaatatgc gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc
     5941 tccgccaaat agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat
     6001 atcattcatc taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa
     6061 agtgcaattt ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt
     6121 ttatgaataa acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata
     6181 tggggagcta tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac
     6241 ctctttagcc tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa
     6301 caacattgtc gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa
     6361 tcatcccttt gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac
     6421 ttttcataat tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat
     6481 cgaagaatcg tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc
     6541 cgtcaaatga agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga
     6601 tttggatatc gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca
     6661 gatcttctgc ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt
     6721 ggacatccat gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat
     6781 ctaagtttac agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata
     6841 aagtaactgg tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt
     6901 ctttgatgat ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt
     6961 ccatagtgat tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt
     7021 ttacaataat tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa
     7081 tccgtgttag gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc
     7141 aagtgattca ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg
     7201 agtatactga cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt
     7261 gtcgatctgc aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt
     7321 aaaagtacat tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat
     7381 tggcaaaaaa atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta
     7441 acaatcgaag aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt
     7501 gcttttcttt accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg
     7561 aaactccaaa cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag
     7621 atgagcagcg ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt
     7681 ggtggacaac gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg
     7741 atcttagatg aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt
     7801 cttcaggatt ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag
     7861 atcaccaccc atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta
     7921 aaacaagcat tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct
     7981 caacaaaaac aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga
     8041 tcaacgatgg caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca
     8101 gcttcagcaa gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca
     8161 gtggaaacaa ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg
     8221 accatccgct ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact
     8281 ttattaaaaa cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat
     8341 aacacaaaaa tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca
     8401 ctacaatatt taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta
     8461 gctaaagcag ggttagttaa tcaattagca agtgaacctc tcactacatt aagcggtggg
     8521 gaacaaacta aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc
     8581 gatgaaccga cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag
     8641 aaatttccag gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg
     8701 caacgagtca ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag
     8761 ctgtgacaaa agttttgtca caactccttc ttctgaataa atggtggcca attagctgac
     8821 cttcggtatt agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt
     8881 attacttgat gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat
     8941 ttctctttcc tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc
     9001 cgctaaccgt tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac
     9061 cagccgcacc tccaataaag tacataaaca taaataca
//
LOCUS       DAJSMI010000105         9011 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJSMI010000105 DAJSMI010000000
VERSION     DAJSMI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.106"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(67..1872)
                     /gene="glmS"
                     /locus_tag="ON430_001971"
     CDS             complement(67..1872)
                     /gene="glmS"
                     /locus_tag="ON430_001971"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR3219881.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINCLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2281..2571
                     /locus_tag="ON430_001972"
     CDS             2281..2571
                     /locus_tag="ON430_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219882.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2584..3609
                     /gene="sppA"
                     /locus_tag="ON430_001973"
     CDS             2584..3609
                     /gene="sppA"
                     /locus_tag="ON430_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR3219883.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEKGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3612..4271
                     /locus_tag="ON430_001974"
     CDS             3612..4271
                     /locus_tag="ON430_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR3219884.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4428..5360
                     /locus_tag="ON430_001975"
     CDS             4428..5360
                     /locus_tag="ON430_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219885.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            5514..6803
                     /locus_tag="ON430_001976"
     CDS             5514..6803
                     /locus_tag="ON430_001976"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR3219886.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            7050..8300
                     /gene="clpX"
                     /locus_tag="ON430_001977"
     CDS             7050..8300
                     /gene="clpX"
                     /locus_tag="ON430_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCR3219887.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            8383..8970
                     /locus_tag="ON430_001978"
     CDS             8383..8970
                     /locus_tag="ON430_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR3219888.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         3003 a         1574 c         1902 g         2532 t
ORIGIN      
        1 attttcataa aaaataaaag aacaaggacc agtgtcagat gtctgacact ggtccttgtt
       61 cttttattat tctactgtca ctgatttcgc taaattacgt ggtttgtcaa cgtcgaatcc
      121 ccgtaatagc gttgcgtaat atgcaagtaa ttgagtcggt acgactgtgg ttagagtagt
      181 tagataaggg tggacagttg gtagcaccag ctggtcactt tccttttcta atccctctga
      241 gacaataact agtgttttag cgccacggct ttcgacttct ttcaggtttc ctcgagtatg
      301 cgcaccagtc acttcttcac tgatgattcc gatcactggt gttccttctt cgatcaaagc
      361 aatcgttcca tgtttcaatt cgccagcggc aaaaccttct gcctggatat aactgatttc
      421 tttcaatttc aaagcagctt caagagaaac gttgtagtca gcaccacgtc caatgtaaaa
      481 ggcatttctt gtatcgctga gatatacttc tgccaactct tctaaataat ctttttgggc
      541 gatgaccgat tccatcgcat tagcaactaa actcaattca tgagtgatat cgaatacaag
      601 agaagtttca aattcttttt tatcaccgat agctttggct aataaagtta gtacagctat
      661 ttgtgcagta taagctttcg ttgatgctac tgcgatttcg ggtccagcat gcaatagcag
      721 tgtaaaggat gcttcccgcg aaagagtaga accagcaaca tttgtaatcg taagtgaagg
      781 gtagccgaga cagttgattt ttaccagcac ttggcggctg tctgctgttt cgccactttg
      841 actcaagaaa ataaagaatg gtttttttga aagaagaggt gtgttatagc caaattcact
      901 ggctaaatga acctcaacag gaatctgtgt caatttttcc aaagtctgtt tacctgctaa
      961 ccccgcatgg taactagttc cgcaagcaac gatatagatt cgatcgctat cagacaatgt
     1021 atcgaccagc ttttgatcaa gagagacacg attgtcttct gttacgtatt tttgagcgat
     1081 acgacgcatg ataataggct gttcgtcgat ttctttcagc atataaaaag gataggtacc
     1141 tttctcgata tctgaagcat ctacttgtgc ttcgaatgaa gaacgttcta cgatgtcacc
     1201 tgcaaaattt tgaataataa ctttttctga tgtgacggta accatctctt catcttcaat
     1261 ctcaacgaat tccttcgtat gagcaagcat agccattgca tcgctggcaa tcacattgaa
     1321 tccatcgccg agtccgatta atagtggact tttattttta gcgacgtaaa taacatcagg
     1381 tgctgtccga tcaatcaaag cgaaagcgta agaacctttg atgacccgca aagcttttaa
     1441 aaacgcctct tgcgttgtag tatccttcgc gaaagtttca attagatgag cgataatctc
     1501 agtatcggtc tcaccaataa agtggtcatt tgctaaataa gcaattttaa gttcttcaaa
     1561 gttttcgatt acgccgttgt gaacgagcac gaagcgtcca ctttgggaag tatgtggatg
     1621 ggcgttttct tcagaaggtt gcccgtgagt tgcccatcgt gtatgaccaa tacctatatt
     1681 tcctgtagta tcaatattta ttttgttttt caaattttga attctgcctt gtgctttgac
     1741 cagataatca gttgtgccat ctgagacaaa gataccagct gaatcataac cacggtattc
     1801 taatttttct agaccgtcaa tcaatcctgg tgttacattt tttaatccaa tcattccaac
     1861 aattccgcac ataaaaaaca tcctactttc tataatgaat tcgtatgaat gcttccagct
     1921 gcaggaaaat ccttttgttt tatctttgca gacatttttt gtggattttc tgtagagtgc
     1981 agtccctagg gccatccgcc gaatctttcg ataaaccctc tcctcgtcat ctgtttacac
     2041 agacctggcg ctatcctaaa aggtataact atcctccttt ttgtttttta tctggaaaca
     2101 tttaaaatca taaccttttg cgaatgttct tgtcaacaaa aaaagaataa aaaataaatt
     2161 ggtatatatc aaattctttg tcaaatgtcc tttttatgtc aaaaaataat gggtttttat
     2221 ctatcatctt tttcaggagt tcggtacaat agaaagtaat cggatagagg aggagtaaaa
     2281 atggattcaa aaattgagat catgacactg ggaatgttaa aaaaacaatt gtccaaattc
     2341 gaagcatcag ctggtgtatc agatgatacg aaaatatttt tagatactgg ctgggatagc
     2401 atccaagaaa tcgcacctga tgccttggaa gttgctcaag ctcgtgagtt tacggtagaa
     2461 gatgaattaa caaaagagtc gttttccggg tatgcgcggg aagaaaaagc agagcggttc
     2521 gacacttctg agcagtcaga aactgtgatc gtcataaaaa acttgtatta gaaaggagaa
     2581 agaatgaata aaagacgttg ggcagcagtt ctgattgctg caggattatt tgtggtttcg
     2641 ttagtttcag ctagtttgac taagcctcag gaaaacgatc aaattgaggg attgaacagt
     2701 tggttatatg gggatgaaga actagctcca aatgtgttgg aaaagggaga cagtacgcaa
     2761 aaaatcgtaa aattatcagt agatggaacg attgctgcgg gagggtcttc aggtttattt
     2821 gcttctgaag gttacaatca taaaaatttt atggaacaac tgaaggcgat tgaagaagat
     2881 ccaacagtca aaggtatctt tttagaagta aattctcctg gtggcggtgt ctacgaaagt
     2941 gcggaaatcg ctaagaaatt ggatacgatt cgtaaagaac atgatatccc aatgtatgtc
     3001 agcatgaaaa acatggcagc tagcggtggc tactatattt ctgctcaagc agataaaatc
     3061 tttgcaacag aagaaacagt gaccggctca attggtgtga tcatgtcagg attgaattat
     3121 tcaggacttc tagaaaaact gggtgtggaa gatacaacag ttaaaagcgg agcgttgaaa
     3181 gatatgggtt caggaacgcg accagagaca aaagaagaag aagcggtgct tcaagcatat
     3241 atcgacagcg catatagtcg ttttgtagca atcgtcagcg agggacggaa taaatctgaa
     3301 gaagaagtca aaaaaattgc tgatggaagg atttatgatg gtgtccaagc aaaagaagta
     3361 ggtttagtcg atgaattggg atttcctgaa gatgctttaa aagctatacg aaaggaacaa
     3421 cagcttgaag atgcggaact agtagaatat tcttccacgc catctgggtt tgctagtaca
     3481 tggttaggaa gtaaactagc agaattccag ggattgaagc cttctgagac tagtcagatc
     3541 ttatccgttc ttgagaatct agggacagcg gaatcaccaa aagcaatgta ttattacgga
     3601 ggtgagtaag catgtcagag ctttcaaatg aaaataagga acaagaaaaa gataagcaac
     3661 agcagcctgc tgtaggacct gactccaact tttcccaaaa ggtactgagc tcttttgaaa
     3721 gagagccttt gacgcccgaa gaaatcaaaa agaaacaacg taaatggaaa aagtatacgg
     3781 acacaagaaa tgaaccgcca aaagcaaatg attttcctaa gtatttctat gcaggttttt
     3841 ttatccgaat gtttgccttc ttggtcgatc ttttgtgtat cggagctatt acgcgtttaa
     3901 cacttggtat tgccaaaaat ctaggttggc ttaatttttc agattcttat ctgagtctct
     3961 acggattcac agccttactt atttatctag cgtattttat tttattgacg aaattcaatc
     4021 atggccaaac gattgggaaa atgatctttg gtattcgagt catcagcttt gatgaagaag
     4081 aacttagctg gtcaaccgtt ttagttagag aaggtgcttg ccggttcatt ctaaaatttc
     4141 ctttgctgat gttagggtat cttccagcag tctttaatca gaaaaagcaa catctgggcg
     4201 attttttcac taatacaagt gttgttacga tcaatctgat caaagcgttc aaccaggaag
     4261 tcaacagctg atgagcaata aaaagcagta acagttgaaa gcaccttcta aaagtcagat
     4321 ctaaaatcta gcttttagag ggtgcttcag ttatttttaa ttgtcatgct ttttttgttt
     4381 tgctactatt gaacatgagc tgaatatgag caagaggagg acactgcgtg aagccgattg
     4441 attttccaga aaaatatgag aatttaatgc gagtggcaca acaagcacta gcaaaccagc
     4501 aatatcagca ggcaaaagaa ttatttcaac gagcttacga gttgaaagaa agctttgagg
     4561 ctaatagcct gcttgttttt tgtctttacg agttggacga gaaaaaagaa gcattgaagc
     4621 aagcactctt gcatgaaaaa cagtatcttc aaaatgagga gtttgctgaa ttttattttg
     4681 acttgttaat cagtacacaa gattttttat atgctagaaa attgatagca agtacagatt
     4741 tttatgaagc tttcgagcag cgggtcattg aaaaaatcca atttgcagaa gaattatccg
     4801 gacaaatgga gaggcagaag atcaaggcac tccacaataa atcggaggaa cttccttcgt
     4861 tggaacctgc acgtcagctt gcgtctattg atgagatcga acagcttcct tatcatgagt
     4921 ttatccaaac agcgagtaaa cttattgtat cgccagaggt ccatatttta gcaagggcaa
     4981 aattactgga gacacttagg caattgaacg aatgccatcc tgttttctat ttgacgattg
     5041 aagaaaagtt agtcaaagtc ataccaaaag atctgccaaa gccgcagcag caggcaagtt
     5101 accagcaatt atgtgtattt tcagatcgtt acgggaatga agatgcgctg cttagttccg
     5161 tgttaaaaga agaattcacg ttgcaatctg caattgttta tcctgtctat gatacataca
     5221 tcgaagaccc gaagcgctgg tttcaactga ctgtagaggc gtgtacagga aaaacgatgt
     5281 atgaaggtac agaagatgaa aaacaagatt ttttaaaaaa aagagaaaaa attttgcagc
     5341 aaatgatctt cttccattag attttatcag tgaaaaagat aaaaatgtta tgaaaaacaa
     5401 ggattctatc cattcaatat gtttacaatt tgaaagggtt agtgtactat ttaaaggtat
     5461 gcatgaaacg tttttgatgt ataaaatgaa tttctaaact cggagggaat taaatgactg
     5521 caacatttga aaaaaaaggc acgaatgatg gtgttttaaa atttacgatt gcgcaagacg
     5581 aaatccaaaa aggattaaca actgcgttta ataaagtgaa aaaaagcttg aacgtacctg
     5641 gattccgtaa agggaaagtt tctcgtcaag tattcaaccg tatgtacggt gaagaagctt
     5701 tatatgaaga tgcattgaat gctgtattac cagaagctta tgaagctgct gtcaaagaag
     5761 ctggcatcga tcctgtttct caaccaaaaa tcgatgttga aagcatggaa aaaggtcaag
     5821 actgggtaat cactgctgaa gtaacagtga aaccagaagt aaaattagga caatataaag
     5881 atctagttgt tgaaaaacaa gaccgtgaag taacagatgc tgacgtggat gcacgtatca
     5941 aacgtgaaca agaagcacaa gctgaattag ttatcaaaga agatgctgca gctgaaaatg
     6001 gcgatacagt agtaatcgac ttcgaaggct ttgtagatgg cgaagcgttt gatggcggaa
     6061 aaggcgaaaa ttactcactt gaattaggtt caggctcatt cattccaggt tttgaagatc
     6121 aattagttgg tcataaatca ggtgaaagtc tagatgtaaa tgtaactttc ccagaagatt
     6181 atcaagcaga agatcttgct ggaaaagaag cagtcttcaa agtgacaatc catgaagtaa
     6241 aagcaaaaga agtgcctgaa ttagatgacg aattcgcaaa agatgttgat gacgaagtat
     6301 caacattaga cgaattgaaa gaaaaatatc gcaaagaatt agtagaaaca aaagaaaaag
     6361 cagcggacga tgcaaaagat gaagcagcga tccgtatggc tgttgaaaac gcagaaatcg
     6421 ttgaattgcc acacgtgatg gttcatgatg aagtacatcg ttcaatggac gaattcttga
     6481 acaacatgca acgtcaaggt atctctcctg aaatgtacta tcaattaact ggttctacag
     6541 aagaagactt gcataaacaa ttcgaaggtg aagcagaaat gcgcacaaga acaaatcttg
     6601 tgatcgaagc aattgcagct gctgaaaatc ttcaagcaac agaagaagaa atcaatgcag
     6661 aaatccaaga attatcagaa acatacaaca tgccaatcga acaaatcaaa cgcgtgttga
     6721 cagaagatat gctggaacat gatgtgacaa tgaaaaaagc aatcgaattg atcacaggta
     6781 cagcgacaga agcgaaagaa tagaaatcaa gtaataaatc atttctgaag aagctacttc
     6841 tagaaaattg tttattcgaa ataagggact gtgacacgat tttgtcacag ttccttattt
     6901 tttgttaaga gtaaaaaatg caaaggtata ggtcagttga tcctttgtta aacacttttt
     6961 ctttttacta aactatgtta tgatagtaaa agactgtaaa gtctttgata gtttcttgca
     7021 tactttttgc aagttaaggg gtgaagacca tgtatgacaa tccaagtagc aatgagaccg
     7081 tacgctgttc tttttgcggg aaaacccaag aagaagtgaa aaagatcgta gctggtccag
     7141 gggtctacat ctgtaatgaa tgtatcgacc tatgtaaaga aatcattgat gaagaatttt
     7201 atgaagaagc tgtacgcgaa ttcactgaag tccctaaacc actagaaatt cttgaagtat
     7261 tgaataacta cgtgattgga caagatcgcg caaaacgctc gttagctgtt gccgtataca
     7321 atcactataa acgtgtcaat cagcaagcac aagaaaacac agatgatgtt gaactgcaaa
     7381 aaagcaatat ctgcttgatc ggacctacag ggtctggtaa aacattttta gcacaaacat
     7441 tagcaaaaac attgaatgta ccttttgcaa tcgcagatgc gacaagtttg acagaagctg
     7501 gttatgtggg ggaagatgta gaaaacattt tgcttaaatt gcttcaatct gctgattaca
     7561 atgtggaact tgcagaaaaa gggatcatct atattgatga gatcgataaa attgcgcgta
     7621 aaagcgaaaa tgtatctatt actcgtgacg tttccggcga aggtgtccaa caagccttgc
     7681 tgaaaattct agaaggaact gttgcaagtg tgccacctca gggcggacgt aaacatccac
     7741 atcaagaatt tattcagatt gatacgacga acatattgtt catcgtaggt ggtgcatttg
     7801 atggcatcga aacgattgtg aagaatcgta tgggcgaaaa gacgattgga tttggtacac
     7861 aaaaccaaaa actttctgaa gacgaaagtg tcatgcagca tatcattcct gaagatttac
     7921 taaaattcgg attgatccct gagtttatcg gacgattacc tgttatggca gctttagaga
     7981 aattgacaac agatgatctg gtacggatct tgactgagcc gaaaaatgcg ctagtcaaac
     8041 aatatcaaaa acttctttct ctagataata ccgaacttga attcgaacct gaagcgttga
     8101 gagccattgc gaaaaaagca atcgaacgca ataccggtgc tcgaggactt cgttccatca
     8161 ttgaagaaat catgatggat gtgatgtttg atatcccttc aaatgaaaac atcgaaaaag
     8221 tgattatcac aaaagaagca gctgaattgt ctggtgagcc aacagtaatc tataaagatt
     8281 cagagaaaaa agcgggttga tgaaaagagg ctgccgtaaa agtcattttg gcttttgcga
     8341 cagccgcttg ctttattcat caccttgtta aggagagaga ccatgaaagt ccatcaagct
     8401 gaaattatca taagtgcagt ggcaccaaaa caatatccag atacacaatt gccagaaata
     8461 gctctggcag gacgatcaaa tgtagggaaa tcttctttta tcaacacatt gattgatcga
     8521 aagaatttag cccgtacttc cggtaagcca ggaaagaccc agacattgaa tttttatttg
     8581 atcgagaacg cattacattt tgtcgatgtt ccgggatatg ggtatgcaaa agtatccaaa
     8641 actgagcgag caaaatgggg aaagatgatc gagacatatt taacgactag agaccagcta
     8701 cgtgctgtcg tttctttagt agatttgcgt cataagcctt ctcaagaaga tatccagatg
     8761 tatcaatttt tgaagtatta tgatatccct gtaattgttg ttgccactaa ggcagataag
     8821 atccctcgtg gtaaatggaa caagcatgag agcgaaatca aaaaagcgtt ggattttgat
     8881 cgcaatgaca cgtttattct cttttcttcc gttacaaaac agggaaaaga agaagcgtgg
     8941 gcagcaatcg aacaagcaat tgaatcataa aaagaataaa aaagacttac cagtcaaact
     9001 ctttcataaa a
//
LOCUS       DAJSMI010000106         8985 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJSMI010000106 DAJSMI010000000
VERSION     DAJSMI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.107"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            78..776
                     /locus_tag="ON430_001979"
     CDS             78..776
                     /locus_tag="ON430_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR3219889.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            954..1295
                     /locus_tag="ON430_001980"
     CDS             954..1295
                     /locus_tag="ON430_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219890.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1288..1959
                     /locus_tag="ON430_001981"
     CDS             1288..1959
                     /locus_tag="ON430_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR3219891.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            1965..2651
                     /locus_tag="ON430_001982"
     CDS             1965..2651
                     /locus_tag="ON430_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219892.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            2654..3403
                     /locus_tag="ON430_001983"
     CDS             2654..3403
                     /locus_tag="ON430_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3219893.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            3415..3684
                     /locus_tag="ON430_001984"
     CDS             3415..3684
                     /locus_tag="ON430_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219894.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            3689..3853
                     /locus_tag="ON430_001985"
     CDS             3689..3853
                     /locus_tag="ON430_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219895.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            3846..4148
                     /locus_tag="ON430_001986"
     CDS             3846..4148
                     /locus_tag="ON430_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219896.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            4145..4306
                     /locus_tag="ON430_001987"
     CDS             4145..4306
                     /locus_tag="ON430_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3219897.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            4303..4608
                     /locus_tag="ON430_001988"
     CDS             4303..4608
                     /locus_tag="ON430_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219898.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            4608..4964
                     /locus_tag="ON430_001989"
     CDS             4608..4964
                     /locus_tag="ON430_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCR3219899.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            4951..5169
                     /locus_tag="ON430_001990"
     CDS             4951..5169
                     /locus_tag="ON430_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219900.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            5166..5585
                     /locus_tag="ON430_001991"
     CDS             5166..5585
                     /locus_tag="ON430_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCR3219901.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            5582..6139
                     /locus_tag="ON430_001992"
     CDS             5582..6139
                     /locus_tag="ON430_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR3219902.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            6136..6432
                     /locus_tag="ON430_001993"
     CDS             6136..6432
                     /locus_tag="ON430_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219903.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            6509..6922
                     /locus_tag="ON430_001994"
     CDS             6509..6922
                     /locus_tag="ON430_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR3219904.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            7231..7383
                     /locus_tag="ON430_001995"
     CDS             7231..7383
                     /locus_tag="ON430_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219905.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(7380..7655)
                     /locus_tag="ON430_001996"
     CDS             complement(7380..7655)
                     /locus_tag="ON430_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219906.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            8109..8315
                     /locus_tag="ON430_001997"
     CDS             8109..8315
                     /locus_tag="ON430_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219907.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            8511..8678
                     /locus_tag="ON430_001998"
     CDS             8511..8678
                     /locus_tag="ON430_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR3219908.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            8704..>8985
                     /locus_tag="ON430_001999"
     CDS             8704..>8985
                     /locus_tag="ON430_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3219909.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTA"
BASE COUNT         3288 a         1392 c         1944 g         2361 t
ORIGIN      
        1 aattgttaag tgaaaaaaat tcaaaataaa caccaccagt ttttaactaa attataccaa
       61 aaaggagaga agaaataatg caagaattag taattttgaa aaataaagaa gctgtgacta
      121 cgagcttaca agtggcagaa agcttcgaga aaaaacatca acatgtttta agagatattg
      181 atgcactaaa aaaagatgtg tccaattttg gacagatgtt tgtagaaggt aatgaaccag
      241 attcatatgg cagaaatcga cgagttttct tcattagtag agatggattt ttcttgctgg
      301 ctatggggtt tacaggaaag aaagctatct acttcaaaca aaaatacatt gaagcattca
      361 acgaaatgga agatgttatt cgcaagaata ctgttcctca aacaattgaa gatatgatga
      421 tctatcaatt agaagaaatg aaagatgtta aaaaagatgt ttccatgctt aaagatacta
      481 tgcgaattag cggacaacaa gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg
      541 gagttctagg gggaaaagaa agccgagctt atgaagaaat cagcaaaaaa gtattctcaa
      601 aattttggtc tgaatttaaa cgtacctttt caatcccaag atatggcgag ttacctcgta
      661 agagattcga tgatgctgtt tcatttattg aaatgtggtt gccagaaact gcgatccgta
      721 tggaaattga tcaactgaac agacaacaga gactttttgg tgatgacaat gaatagagct
      781 gaagcgctaa gaatagggac ggcaattgct aattgctggt ggaaatacta taaaccaatc
      841 atcctaagcc aacaacatat tgacaagcaa aaagcatggc aacaaataaa aaagtgactc
      901 cgccggcaag caaagagtca caaagaaaac acatcataag gagattttag catatggaaa
      961 aagaactttc cactctagat caatatttga ttgatcctga ttggggcaag ccgaaaattg
     1021 aggaaacaag tggtcgaaaa atcagacgaa atcttttgac gaatgaagaa ctagcttggg
     1081 atcaagatga tttaggcaac catgtaacta tttgggatca tgtttatctt atccatctat
     1141 cgaagcattc gaataaacct gaatatattt acgtcatcga agatggcttg attgatgcgc
     1201 tagaagagta cgacagagat aacttgattg atatctctta ttacggacca ggtaagaaat
     1261 acattgctga aatggaggca gaatttgatg agtgaaatca aagggacaac gaactttgaa
     1321 aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa aaggaaattt tgattattta
     1381 tcttgggctc acgcgtggga gattatgaaa aagaatgatc cacaggcaac ggtaactatt
     1441 aatgagtaca aacactacag ggttgtttct ggaactcatc aagactttct tgttgaggaa
     1501 tataaacctt ttcttatgga tgaaactggg acttatgtat ctgtctcagt aacggttaaa
     1561 ggacacacgg aaaccgaatt atttcctgtt ttagattatc gaaaccaacc agttgttaaa
     1621 ccaaatgcaa tgcaaatcaa taactcattg aagcgatgct ttgtgaaagc attggctcta
     1681 cacggactgg gattatatgt atttcaaggg gaagatattc caacaccacc tagaatcgat
     1741 acaaagaaat taaacatgct agagacgatt ctagaagctt tcaatgagca gatgggtaaa
     1801 gatatgacca aaaccttaat cgaatatgtt aatgagcaga cagataaatt agggctctta
     1861 gctgataacg ttgaaactat tgaacagtta agctatgagc aatgtgcctt gatggagcga
     1921 gcaatagcag ctaagagaaa agaattagat aagaagtgat atgagtgttt aaaccattaa
     1981 tcgattcata ttcagcggtt ctgaaaaagt tcaaaggaaa agacataagc gcaaccatca
     2041 atgaggaagt gaacattgat cgactaaaga cgatgtatga cggctacgat ggtgatcgag
     2101 tcattgaaat tcgttttatt gatcctagac gtttcaccgt acagcaacga aacttcatct
     2161 atgcgctgat aggcgatatt tttatcgata caggcatgcc aacggacttc tggaaggaat
     2221 tcttctactt tcgttttgaa ggtgtcacag ggcgcaaaat aagcctgaaa gacgaatcga
     2281 atacaactgt gagtgatgcc aatgtcttag caaatatcat cttagatttc atctttgaac
     2341 atcatattcc tttcaaagaa ggctatgaga ttttacctgc gaatcaagaa tattacttct
     2401 acaaatgcat cacaaaaaga gtctgctgca tctgtggcaa aacaggagct gacatcgatc
     2461 actttgacaa agcgctagga agacgaaagc gcaaagaagt tgatcatgca gagtacacat
     2521 ttgcagcact ctgcagaatc catcacacag agaagcacaa aataggtgtg attaatttca
     2581 aaaataaata tcaaatcaaa gggatcaaat taaaccagaa aacaatcaaa aagttaaata
     2641 ttggagggta aaaatgacag aacatcgaag ttattatgcg attataccag ccaacgtaag
     2701 gtacgacaaa agacttaaac caaatactaa gttgttatac ggagagataa cggccttgtg
     2761 taatgaaaga ggcttttgtt gggcaggcaa tgagtacttt gcagatttat atggtgtgaa
     2821 taaagagacc atatcgcgat gggtaagtga tttgattaag tttggatact tgaatcggga
     2881 aatcatttac aaagagggta ccaatcaaat aatcaatagg tacctacgaa ttaatcaata
     2941 ccctattgac gaaaaacgca atacccctat tgacgaaaaa gtcaaagata ataatacatc
     3001 tattaataat acatttaata atacaaaaga atatataaga gagttaccgc cttcgaaaaa
     3061 atcgaaggct aagcccgtcc gtcataaata cggagagtat aaaaatgttc ttttgtcaga
     3121 tgagcagatg gagaaactca aaacagaatt ccctaatgat taccaagagc gaatcgaacg
     3181 actgtcagag tattgtgaat catctggtaa gacttataaa aactatttgg caactattcg
     3241 aagttgggca aggaaagaaa aaagtgaacc taagaacgca agcagtggat acaagcgcac
     3301 aggaagacga gagaagcttc ctgaatgggc aatcgaccaa gaagcctatc tcaagaaaaa
     3361 agcgctagaa cgagctaata gacaatcaaa agcaccattc taagaggtgg aaaaatgaag
     3421 atcgattatc tagaactaat taatgaaata gcgaattata aaaagggcga ggaattagac
     3481 gtcctgagag acgtatatga tcaactcgaa gaagctggaa ttgaaggaat taagaatgat
     3541 cgttcgagtt ggagtaagct cagatactat ttcgcacttt atatcgatac aacacaatta
     3601 agaaatttag catataccaa attactattt gttgattgtg ttaaaggctt gcaaaaacat
     3661 cttaatgaac ttgagcaggt gtaatcagat ggacctaaag acatttacag cacagattga
     3721 actaatgcat caagaagctt taagacaaag tgtatcgtac gaagacaagt ggctcaacac
     3781 gttccatggc ggacgtgaga gcgcacttga tcaagtactc aaattactga aaggagaatg
     3841 tcgggatgga taagaaagcg gcaatgcagc gaattatcga attgacttat tcagaagatt
     3901 ggcaaaatga caaagaagct gcttcagaag tgatgaggct tggaagagcg atgtgggcag
     3961 acaagagcaa caagccaaga ccacgaaaaa tcgcaatttg gcacggtgac aaacttctag
     4021 tgatagggac agctgaacag ttagcaagtc tcacaggctt gcacgagaaa atcgtgagga
     4081 aaagagcaag gtgtggctac acagacgcta agaagagaac gtttagatac gtggaggaat
     4141 catcatgaca acagaagaag tgattcaaat gcgtattcga agccttcagc gtgagattga
     4201 cgatctggaa cgaacaaagg cagtgatggt caatgaaacg gcgagaaagg caatcgattt
     4261 gcacatagag aatttaagaa gggaaatcca tcgattggag gaatgagcgt ggataagaaa
     4321 gcagcaatga aaagaattgc tgaattaacc aagtcagaat cttggcaaga agacaaagaa
     4381 atagttgcag aagtccaaaa gctcggtaaa tcaatgtgga ctgaaaagcc caaacggaga
     4441 acgccgagaa aaattgcaat ctggcatgat gaccgaattt tagtaacagg tactgctgaa
     4501 cagttatctg aaattactgg attaagcaaa aacattatct gggatagagc taggagctta
     4561 tggattgatt caaaaggacg acagtttagg tatgtggagg agagataatg gatctcatta
     4621 cacaatacag tgacatcatc ctcaagaaaa tcatgatgaa gattcagaaa gataaaaaat
     4681 caaaagaacg agctgaatta gttaagttag aaatggctga aacaggagca ggagtgcgaa
     4741 gtagcaggca ttggaaagca gcagcaaaca ttgaatttta ttacaacgaa attcaaaaag
     4801 ggttcgatca gatgcgtgag ctggatcggc aaacaaattg gagcaagaaa cttcatcaag
     4861 atcgtttcaa atttgtagag aagtataaag aaatattaga agagtatttg aggaggacag
     4921 caaatgataa aaaaactcgt tcaattcagc atggatttat atgatatcga atcaggagca
     4981 acactatctg tggaatcgga ccatctaatc ataaattttg gtggaaagcg ccagattatt
     5041 ttgtgggtag ttgatgatgt actgtttcca gaaattgttc atgatttcga agaatcaaaa
     5101 gcggttgagt ttgaaatagt gaaaaaagta atggaattga ttgaaaaata cgaggaggac
     5161 agcgaatgat accgaagttt agagcgtggg ataaacgaaa gaacgtaatg agagatgtag
     5221 ccgtcttgca ttttactaaa aacggcaaaa caaactttat tgaatattgg ataaatccta
     5281 ccgaattgaa atcatatcac gtgcgaaaca tcgacctcat gcaatccaca ggaatgaaag
     5341 ataagaatgg tgtggatata tttgaagggg atatagtatt agtcagcgtg cgaaatggct
     5401 tcgattactt agataataaa gtctgtattg tcaaaaattc aatagattat tccggattag
     5461 tttgtgccac tgttgatgaa gacttagagt atcaaatttt taacacagag ctgtttgaag
     5521 aatacacgta cgaagtcacc ggaaatatat acgagaatag cgagttattg gaggaacagc
     5581 gatgaataaa caggaagcaa tcaaaaagtt agaaagtatt aaagcgatag gaaatgatgc
     5641 aatagctgct tgctataacg agagtataaa ttcaggtatt acgttaatga aaaaaataga
     5701 cgaaccgcag aaaccagtta ttccacaact cgtggccggt tggcttgaga aatctacgga
     5761 cccttttaca aaagctgaaa aaatagcgta tttaatcaaa tctaaagatg gtgattcata
     5821 ttatttctgt gattggtttg tacgagatgg catagtgacg caagagcaag gagaagaatt
     5881 actcgcttgg gcaacaagac aatcatatga aacactattg agcctataca acggctacga
     5941 ggttgagaaa gagccgttat gggcaataaa gaatgccgat ggaaactatc ttactaaatg
     6001 tgctttatgg ggaaaagatg gagtaaatta tagttttgaa tgcaatccat ctcatcgatt
     6061 gcttttcact gataaagcaa cagcggatgc tgcagcattg ttggtgaatg gaacagtgga
     6121 agaggtggta gaaagatgaa actaaaagac ggattttacg ctagcagtca cggtatcggc
     6181 ggtttaatgc tagatatgcc gacaaagaac cctaaaacac gcaagaaatc aaaattcaaa
     6241 gtcggtgaca tggtccgctg tgaagcagaa gagttcgttt atccgttcag aggatacgtt
     6301 aaaaagatac tgtcaaactc agcaatcatt cgtattgaaa atacaatgaa gtgtgatcgt
     6361 tcgactgcaa aacacaagca ttatttagct gtagcgagat tagttgacat ggaagtaatc
     6421 aagagcaaat aaaaaaagcc ggatcgctcc gactgattca ataaatccaa cacatttatt
     6481 atatcacata aaggagcggt ttgacttgat gcaattgtta cgagaggtag atttcaaaca
     6541 gacaagatgt aatgcgagag atgtgctgaa gaactttcgg cgtttggagc ggatggcagg
     6601 tcgctctttg atagatatta agtcgccgat tataacggat atgccgaagg caccgaagca
     6661 tggcaataag gcagaagacg cgattattca gatgatggat atagaaacgg agagagatgc
     6721 gattctagcg gctttgatgg ctcttagtct gattagtcgt cagatactct actacagctt
     6781 ctgtgtgcca gatagcttct caaactacag aattagccgt gaagtgggtt attcagaaag
     6841 aagtatacaa cggatgaagt cggaagctct aatagagttt gcagaagcat ataaacacgg
     6901 aaaaataatt gcttataaat aatttggcgg ttttttggcg gaatgatggc ggtttttaga
     6961 catttaccag tgttattatg gtagtgtcga aagattagga aacaggatcg acaaaataaa
     7021 cgaagggagg caatctccct catcgtttta aacttcttct tagatagaca gcaactaatt
     7081 atgaagaaaa ggatgtgaat tcaactcctc ctaaattctt catcggttgc tgtttatttt
     7141 gattagacag cagcactcac aaaactaaac atgttcttgt cgtctttcaa ctgctgctgt
     7201 ctattaattt atgtattgga gggaagaaaa atgaaattat cagtagaagg cactccagaa
     7261 gaaatacaag aattgctcca agctattggt agtagcaagg agcaaaaggt aagtgtttct
     7321 aattcagata ttgacggtat ttgcaattgt ttaaacagag aaaaaatatc taatccttac
     7381 taagagaaaa tatgttcgtt aatgaatttc caattttcct ctgtagctct accttgataa
     7441 tttttttgca gttcacagat gaataaggaa tcgttattat caagtactgt atttaatttt
     7501 tcacacattt gatttggatt caaattagtt ttaactaacc aaaaagattt ttgtagcttt
     7561 atatatgccc caaattcttt tattatttca aatactttat catacttttg ccctgggtta
     7621 ttaagatcat aactaatcat gtagggttta tacatgttgt cttgcacatc cttgttttat
     7681 atttcagcgg accactcgct gataaataaa attatacgct tagtatttat tttcacaata
     7741 ttaatttatc gtaataactt ttgagtaaat agaacaaaaa acctgcacta gtttccgcta
     7801 gtacaggcag tgactatatc ggttgataat ctagcatata ttaaaaataa ttgcaagaaa
     7861 agaaatttat agtggtttat accaaattat caaagcaatg ttattttgtt gctgtctatt
     7921 gttttttaat tattcacacg ataactaaag gtgggtgaag agaaatgatt ccattaataa
     7981 tttcaatttt tgcgctctgt cttaatgtct atatgattgg atttaaaaat gggcaaaata
     8041 aaaaatagta gcagccaaga ataattttat agtgtcactg tggcggaaag ggtagacgct
     8101 aagcatgtgt gctaggtcaa tgcttcggca accatgcaag gttcgattcc ttgccagcga
     8161 cattaaatgc ctatgacggt tacgactacc gaaaaaagat cgttaagaag ctatacggtg
     8221 ctacgtacgg caatgtagta agtgtgctat ctgtacacca ccaagcttcg gtcactgtgg
     8281 cggaagtaga agacgcagcg gtaaatggcg agtagcctcg tgagagcctg gtaagttctc
     8341 gtgagtggtg caatcccact ccagcgactt taagcaaccg aggcatcggc ggtttaaaaa
     8401 tataggggtg cgcaatttcg tacgcgtttt gtgcatcgtg caagttacta ttacatatta
     8461 gatcactctt tgagtggtct ttttattttt gcacaaagga ggaaacaaca atgtatagac
     8521 cacaatactt agaacagaag tatgaagtaa tcactgttca taaaggtaat ggcgaaaaag
     8581 tatatgagta tagaagacca ataaagagcg atacatataa acgaaaggaa aacaatgaag
     8641 ttattccatt gtatggcaaa agaatagcta agcattaaat aagattgcga aaggagacgg
     8701 aacatgaccg aggaattcta tagatggcta ttacagttga caagagaaga tcgtttggtt
     8761 aagttctatc agtctcctaa atggcgcagg cttagagaga aagcgatgaa acgagatcac
     8821 tatgaatgcc aagagtgtag aagactaggt aagtatcata gagtagagaa cgttcatcat
     8881 ataaaggaag tcaaggatag acctgactta gctttagatt tagataatct tatttgttta
     8941 tgtgttgaac atcataatga agttcatggc agatatctta cagca
//
LOCUS       DAJSMI010000107         8875 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJSMI010000107 DAJSMI010000000
VERSION     DAJSMI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.108"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(488..712)
                     /locus_tag="ON430_002000"
     CDS             complement(488..712)
                     /locus_tag="ON430_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219910.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            903..3680
                     /locus_tag="ON430_002001"
     CDS             903..3680
                     /locus_tag="ON430_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3219911.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3761..4180
                     /locus_tag="ON430_002002"
     CDS             3761..4180
                     /locus_tag="ON430_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCR3219912.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4192..4662
                     /locus_tag="ON430_002003"
     CDS             4192..4662
                     /locus_tag="ON430_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3219913.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4679..5458
                     /locus_tag="ON430_002004"
     CDS             4679..5458
                     /locus_tag="ON430_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3219914.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5445..6266
                     /locus_tag="ON430_002005"
     CDS             5445..6266
                     /locus_tag="ON430_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3219915.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6283..7332
                     /locus_tag="ON430_002006"
     CDS             6283..7332
                     /locus_tag="ON430_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3219916.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7345..8349
                     /locus_tag="ON430_002007"
     CDS             7345..8349
                     /locus_tag="ON430_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3219917.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT         2962 a         1468 c         1835 g         2610 t
ORIGIN      
        1 tttaatttgt tcttgcaatc ttttttgcta cgtaattttt gtaaaaaagt gggttgagag
       61 aaatctaatt gattttcaaa gaaggagtat gaaaatgaga aaaatattta ttcgtttatt
      121 gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg gcgcagattc
      181 tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagct acttgagatt
      241 ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta ccttgttttc
      301 gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga tggtgacaac
      361 agtacctctt tactctctga aagcatcata actcgttttt cctcattatg ccaataaatt
      421 gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa gttgaaatct
      481 gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata aagatgaata
      541 aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt tgttctgtca
      601 tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg atgatttctc
      661 gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc attgaatagt
      721 catagcctcc ttattctttc atatgttgaa attccagtat aaaaactgga aggaactgta
      781 cgttttttag aataaaaatt ataagggata agtaggctgc tgtctataat aagatatatt
      841 tttcaattct ttattaaaga ttttattgac aaaatgaatc cgttttctat gatggaggag
      901 gggtgaataa gttgttgaag aaggaaattt tatgttactt agagaaccag acagcctttt
      961 tcgatttaga acatatgaac gaggtcttta cagcacaaag gttagctgat cattttgggg
     1021 taaaaagaaa cacagtcagt cattatttga accagttaaa tgatgagcaa ctactagtca
     1081 aaatcaattc tcgaccagtg atttatttcc acaaagaagc ttttgaaaaa caatttttta
     1141 agttacgcac gaatttttat ttttctatcg atgatctcaa agaggaacag ccttttttcg
     1201 cgcaaccaaa agacttattc tcttttatga tcggatacga cgccagtttg aaagaaagta
     1261 tcgaacaaat caaaacagct ctttactatc cagatggcgg attaccactt ttgattacag
     1321 gcgaaagtgg cacaggtaaa agttatttag ttaatttggt ctatcagtat tgtctgcttc
     1381 atgatctgct agaagattct gcaccattca tcacttttaa ttgtgcacaa tatgcagata
     1441 atcctgaact tttgactagc aatttgtttg gacatgttaa gggagcgtac actggtgcag
     1501 aagaaaataa aaaaggggct tttgaagcag cagatggtgg gatcttgttt ttagacgagg
     1561 tacatcgctt aacaccagaa ggtcaggaaa aactgttcac ctatcttgat caaggtgtga
     1621 tttatcgtat gggagaacct aatttgtcac gccggatcaa aacacgtctc ttttttgcta
     1681 cgactgaaga gattactagt catttcttaa caactttcat tcgaagaata cctatccgaa
     1741 tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg gctagaactg atttactctt
     1801 tttttctcga agaacaaaga aaaatgggga gccctttgtc agtcagtgga ccagctttgt
     1861 cacttttaac aagtcagaaa ttatccggaa acataggaga gctgaaaaat atcgtcaaag
     1921 tttcagcagc aaaagcatat gctgaacagc gtgaacagcc tgaaatccat gtcacgatcc
     1981 atcatttgca aaaagaacta cttgcgcagc ctattactaa aaatacagca cagcaaggaa
     2041 tctatattac gcaagaccag accttagagc aattaatgga gaagcaacag cccgaacagc
     2101 agcgaatcgt taaaagtttt gagcaaatcc taattgattt caagaaaaat aactgtctgc
     2161 tatattcttg cgaaggaaaa ttaaagcaag aagtgattca attatttgat tttttgctgt
     2221 ttgagaccag tagacaagaa aaacacgagc tgctggttta ttggacacaa tctatccgag
     2281 agacctttcg acaaatggaa actgcctatc aaatcaaatt cgatggaaac agtgtctatg
     2341 ccttgagtta ctatctctat cagcgaagca ctatcaaatg gttaccggaa gaacatgata
     2401 ttatccaact aatcaaagaa ttagaacaac aagtttctca gtcttatcca tctagttatc
     2461 actatgtcca acgaattctg gaattaagta agatcaaatt agatattgaa gacacaagta
     2521 tggatcggat tttactgacg atctatctaa aaaaagcgaa atggtcaaaa gaaaaacgac
     2581 tgccaaaagc gatgattgct gctcacggtt atgcaactgc tagcagtatg gcaaatgtag
     2641 taaatcgttt attacaagag gatctatttg aatcatttga tatgccacta gatgtcacac
     2701 cacagcagat tgcttcagaa atccttgatt attgcgaaaa cagcgatgtt tcaaatggat
     2761 tgatcatatt agtagacatg ggatctctga aagaaatcca tcagtttttt aaaaaacagt
     2821 tatccgtacc gcttttgatt ttgaataatg tgacgacacc tttagcaatc acagtagggg
     2881 aatgcctgca aaaaaatgta tcattagaag agatagcaga agagactgtt cgacagatcc
     2941 agccagaatg gcgactgctc tatccagagg aaaacaaacc caaagcactc attactactt
     3001 gcttcactgg aatcggtacg gcggtccact taagtgaact gctagaaaaa agcttaccta
     3061 cggcttgtca gttgaaaatc atcccatatg aatatcagca attaaaagat aagaaaaaca
     3121 gtgacccatt attttccata tacgaagtcg taggaatgat tggcaccaca gatcctgata
     3181 tcaccaatat cgactatctt tcattagaag acctcatctc gggggaaaag atgtctgtac
     3241 tagctgaatg gttggacact actatgaatg cttcagagaa ggaaatattc aatcagcgca
     3301 tcattcgtaa tttttcattg gaaaaagtac tggattccgt gactattcta gatacagaga
     3361 aggtgatggg agaaatcgat cattttatga gagaattaga ggtgcagttg aatcgaagca
     3421 tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag ctgtctcatt gaacgattga
     3481 tccgacaaat gccaatcgaa acctatcaag gattagagaa atggaagcag tgtcaaaaag
     3541 aagggttatc tgctattaaa tctgcgttta gtgtcattga agagcattat agtgtcatga
     3601 tccccaattc tgagattgcc tatatctatg atattttgag tgaagataca gagtttctct
     3661 cgatagaaga agaattttga acataagcaa gttgttgcca ctaattttgg catgcaactt
     3721 gcttttttag tatgtgtaag tgaaaagagg agatgaaaaa gtggatagag aaattatctt
     3781 agcttcccat ggaaaatttg cttcagggat attagattca ttagagctga tctatgggaa
     3841 aaatcattct attactgtat tcgattgcta tactgatgag aattttgatt tagcggaaac
     3901 agtaaggcag ctttttatga ggtacaaaga taaagaaatc attgttctga cggatctttt
     3961 tggcggaagt gtcaacaacg aattcttaca atatatcaat cgggatcatg tctatctcgt
     4021 tgccggattg aatttgccac tattgattga gctagtatct cggttggacg ctgaagtaga
     4081 gacaactgtt ttgatccagc aggtgctaac tgattcaaaa aaaatgatcc agttttgtaa
     4141 cgagcggctc aatcagaaaa tagaagaaga cgaattttag gaggaagaaa aatgattgta
     4201 ttgacaagag tcgaccatcg gttattacac ggacaagtag ctttttcatg gacacaaaca
     4261 ataggcgcag actgtatttt gattgctaat gatgatgtac ctacgaatga gatacgaaag
     4321 acaacgatca aattagccaa gccacaagga gtcaaactag tgatcaaatc aattgatgac
     4381 tcgattgccg cgttgaaaag cggggtgaca gataaatata agttattcat cgtagtggaa
     4441 tcgatcgagg atgcatataa gctggcagaa gcgtatccag aaatcaaaac catcaactta
     4501 ggagggatca aggcaaaacc tggtactcgc agcatcggaa aagcggtaaa tattttacca
     4561 gaagaggagg aacgcttgaa acgattaatt gacaaacaaa tagaagtaga aatccgtcaa
     4621 gtaccagcag ataaaaaagt ggatgtaaga gaagttttgt aagaaaggaa gaacctcaat
     4681 gattgtacaa gcagtcttat taggaattgt tgcatttgtc gcacaatcag aatatgcact
     4741 agggacatcg ttgctttctc gtccgattgt gacggggctg tttacaggaa tcgtgttagg
     4801 tgatgtaaaa actgggatca ttatgggcgc aactttagag ttagcattta tcggatcgtt
     4861 ctcagtaggt gcttcgattc cacctgatgt tgtgaccggt gggattttag ggacggcttt
     4921 tgctattaca gctggagccg gtacagaaac cgcgctttta ctaggtttgc cgattgctac
     4981 gttgacattg atattgaaaa atatttactt agggcttttg attccaatta tgaaccataa
     5041 agcagacaat tatgcagaag aaggaaatta taaaggagtc gagcatatgc acttgcttgc
     5101 cggtatcggt ctttccttga tgcttggtgt tgtggtaatg gtttcttata tggtgggaag
     5161 caatacgatc ggaaatctat taaatatgat tcctgacttc gttcaaaagg ggttgtcagt
     5221 agctacaggg ttgattccag ctcttggttt tgcgatgctt gctcgtttgc tgatcaacaa
     5281 acaagttgca ccatattttt tcttaggttt tgctatagct tcttacttag agattcctgt
     5341 gaccggagta gctatttttg gtgcgatttt agcagttgtg gtagtcaata ttatgaatgt
     5401 ccgtcaacta ccacttcaaa caactgcagg ggaggtagaa gaagatgaag aattctgaga
     5461 tcaaaaacag caaaataacg aaaaaagagc taaatcacgt attttggcgt tcttttcaaa
     5521 tggagttttc ttggaattat gaacgtcaga tgaatatggc ttatgtttat gcaatgattc
     5581 cgattttgaa aaagttatat cagtcgaaag aagaaatggc agcagctttg aaacggcatc
     5641 tagagttttt taacacgacc ccacatatcg ttacactgat tttaggaatc acggctgcaa
     5701 tggaggaaga aaataaggat gatcctgaat ttgatgtgac tgcgatcgat agtatcaaaa
     5761 catctttgat gggacctctt gcagggttag gagattcttt cttttgggga actttgcgat
     5821 tgattgccac aggtgtgggt acctctttag ccatgcaagg aaatatttta ggccctatct
     5881 tgttcatttt gattttcaat attccgcaca ttctgtttcg ctatctagca acaggttggg
     5941 gatatcgctt aggaacgggt tttttgaaaa agatccaggc aaacggtatg atggaaagtt
     6001 tgacattagg cgcgtctatt attggactga tggtcgttgg agcaatgact gccacgatga
     6061 tcaccatcaa tattccttta aaaatcggtt caggagaaaa tgctgtgact gttcaaagta
     6121 tttttgatga tatcgttcca aatatcttat gtcttggagc atttggcata gtgttttatc
     6181 tattgaagaa agaagtgaaa ccattaacga tcttgatcgg actagctttc tttggaattt
     6241 ttggttcgtg gatagggatt ttttaaaaaa ggagcagaga aaatgtttac gatgcaagat
     6301 tatatctatg aagaaaagga agtactaagt acgatcttga aaaagaatga tttttcgaca
     6361 agagaacata tgaaaaaaac agtgaattta ctgatcttag caacgggttc atcttataac
     6421 gcttgtttag cagcgaagcc agcattggaa tcttatggtg atcttactgt cgatattcaa
     6481 gaaccttttt attttttcaa ttatggaaaa ctttctcctt caatcgatac tgtgattgct
     6541 gtgtcacaaa gcgggaaaag tgcttctact gttgaagcag taaaaatgat acaaaaacaa
     6601 gggttacctg ttgttgctat cacaaatgac gtgcaaagtc cacttgcttt agaagctgca
     6661 caaattattg atttaggtgc cggggtagaa tcagtaggct tcgttacaaa aggctattct
     6721 gcgactgttt tacagttact actcttaggg ataggaattg gaataagtaa aaataaaatc
     6781 agcaaaaaaa tagaacaaga ctacatgcaa cagttgcgca aaataatcaa ccatttacca
     6841 gcgattattc aaaaaacgga agaatttttt gatggatatc aaagtttatt tcgtttagca
     6901 cagcgtttcg tcaccattgg atatggtcca aactggggaa cagctaaaga agcagagacg
     6961 aaattgactg aaacaatccg cgttccgtca caaggatttg agttagaagc ttatatgcac
     7021 ggcccatacc tggaggcaga tgcttcgcat ttgctattct ttattgaagg ggaaagtgta
     7081 aataaggaac gttcgcaaaa gttgcaaaga tatatgagtc ggtacgttgg agagactctc
     7141 accattacca caaaaaaagc aagaaacgag aaaacacttg gattagcaat agaatgtgat
     7201 gaatatctgt ctgtattagc gcttgtcgtt ccgtttcaac tattcgctta taaaaccgca
     7261 gtagctaaag gtattgactt gaacaaaaag atttttgaag actttgatac agtcttgaaa
     7321 agtaaattat aagaggtgga aatcatgctg aaattcaatg aagaagaaca aatcaaagca
     7381 ataaaaggcg cactagcttt acgtccgcaa gtagaagaaa tcatcgataa actttatgcg
     7441 gaaaaatttg acgctgtcta ttatctagga atcggtggaa cgtatgcttc ctcgatgcaa
     7501 gctgtcactt atatgaatgg gaaaagcaac ttgcctgtct ttgtacagca tgctgcagaa
     7561 tattacacta ctggaaacaa aagattaaca aaagattcta tcgttatttt gtcctctgtt
     7621 accggtacaa ctcaagaagt agtgaaagca gtagaacaaa tcaagaaagt aggagcgaca
     7681 ctgattggat ttatcgataa agcgaacagt aagttgtctc agctatgcga ttttgtggtc
     7741 acttatccag ctcctggaac agaacaaatc aaatttttta tggcagcaga tcgattgatg
     7801 tatttgaatg gagaattcga agcttattca gaatactatg aacagctcga acgttattta
     7861 ccaacagggt tagtggaagc agagaaaaaa gcagatgcat ttggcttatc atttgcagaa
     7921 aaacatcgac gtgatagtat gcattatttt attggagcag ggaatcaatg gggagctgtt
     7981 tattcatatg ccatgtgtta ctgggaagaa caaagctggc ttccatcgaa atctatccat
     8041 gctgccgagt ttcttcacgg gacgttagag atcgtagaag aaacaacccc agtcacatta
     8101 tttttaggcg aagatgaaca gagagtgtta tctgaacgtg tggctaaact tttgccaaaa
     8161 atttgcagca attatacatt gatagacaca aaagactatc cagtagaagg tatcagtgaa
     8221 aaatatcgcg gacgtgtttt gtctttctta ttgatgcatg tagtgacaca acgcatcgat
     8281 gctcatgtgg agcagttgaa ctgccatcca ttagagatac gccgctacta tcgtcagttt
     8341 gattattagg atagactgga taaaaagaat ataaaaaata aagagagcag cgataactgc
     8401 tccctttatt tttttaaaat attggcatca attacgttat aaaacacatt ttttgcatta
     8461 tctacagttc aacttataat ctgtttaatg ggacatgtgg gtgccaacca ttttttgtta
     8521 tgcaatagta ctatttagtt gttgcatgga tccgtagtaa aagtccaagt acaacgagtt
     8581 gcgccagttt acatagcgtt ttgtatccag aatgttgact aaaacaaaag ttcctatgtc
     8641 aaccgtcaac agagacaatc tgtccgttgc aggacctgac agatttagaa atgttcaatc
     8701 tgtctcttat gtattttaaa tcaatactca ctaggagaag tttcctaacc tcctaatagt
     8761 aatgctggta gtaacattgt caaaactgct tcctcttagg aacaagcaaa gcataacgag
     8821 tgtaaaaata aaatgtagac tattgtaaca tatcattgat tatttcaagt aaaaa
//
LOCUS       DAJSMI010000108         8827 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJSMI010000108 DAJSMI010000000
VERSION     DAJSMI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.109"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            108..2705
                     /gene="adhE"
                     /locus_tag="ON430_002008"
                     /gene_synonym="adhC"
     CDS             108..2705
                     /gene="adhE"
                     /locus_tag="ON430_002008"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR3219918.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2934..4040
                     /locus_tag="ON430_002009"
     CDS             2934..4040
                     /locus_tag="ON430_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR3219919.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4057..5085
                     /locus_tag="ON430_002010"
     CDS             4057..5085
                     /locus_tag="ON430_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR3219920.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5091..5570
                     /locus_tag="ON430_002011"
     CDS             5091..5570
                     /locus_tag="ON430_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR3219921.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSWVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5586..6881
                     /locus_tag="ON430_002012"
     CDS             5586..6881
                     /locus_tag="ON430_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR3219922.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6892..7716
                     /locus_tag="ON430_002013"
     CDS             6892..7716
                     /locus_tag="ON430_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3219923.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7881..8780
                     /locus_tag="ON430_002014"
     CDS             7881..8780
                     /locus_tag="ON430_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3219924.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
BASE COUNT         2825 a         1535 c         1962 g         2505 t
ORIGIN      
        1 taactgtgaa aaaaatcaca aaaaagtgtt tacatagaaa aataaatatt gtatgatata
       61 catgtgaatt atttaacaaa taaccatgac ccattaggag gataatgatg gctaaagtaa
      121 tagaaaaaga aaaaacgaag acaattgacg tacaagcaat gattgatgat ttggcagaaa
      181 aagcaaatgc agcattgaaa gaaatggaaa gctttgatca agaaaaagta gaccatatcg
      241 tgcatgaaat ggcaatggca gctttggatc aacatatgcc attagcgaag atggctgtag
      301 aggaaacagg ccgcggtgtt tacgaagata aagcaatcaa aaatatgtat gcgtccgaat
      361 atatttggca taatatcaaa catgataaaa cagttggtgt gatcaatgaa gatgtccaaa
      421 aagggttgat tgaaattgct gaaccagtcg gtgtggtttg cggagtaact ccaacaacga
      481 atccgacttc cacaacgatt ttcaaatcaa tgatcgcgtt gaaaacaaga aatccaatcg
      541 tttttgcttt ccatccaagt gcccaaaaat cttctgcaga agcagcacga gtggtacgtg
      601 atgctgctat cgctgccgga gctcctgaaa attgtatcca atggatcgaa catccttcga
      661 tcgaagcgac ttctatgcta atgaaccatc caggtatcgc gatcgtctta gcgacaggtg
      721 gagctggaat ggtcaagtct gcttactcta caggaaaacc agctttaggt gtaggtccag
      781 gtaacgtacc agcttatatt gaaaaaacag caaaaattaa acgtgcagtg aacgatttga
      841 tcgtttcgaa aacatttgat aatggaatga tttgtgcttc tgaacaagct gtgatcgtgg
      901 ataaagaaat ctatgcagca gtaaaagcag aattccaagc acaccaagtt tatatcgtaa
      961 aaccagatga attgcaaaaa ttagaagatg cagtgatgaa cgaaggaaaa tatgcggtca
     1021 atccatcaat cgttggacat tctgcgatgg aaatcgcaaa attggcagga atcagtgtac
     1081 caaaaggaac gaaaatgctg attgctgaat tggaaggcgt aggcccagat tatccgctat
     1141 caagagaaaa actttctcca gttcttgcca tgatcaaagc taacaacacg gaccatgcgt
     1201 ttgatttgtg tgaaggtatg ttgaatcttg gaggattagg acatacagca gttatccatt
     1261 cagaaaacga agagttgcat gtcaaattcg gtcttcgaat gaaagcttgt cgtattttag
     1321 tgaatacacc atctgcagaa ggcggcatcg gtgatatcta taacgaaatg attccgtcat
     1381 tgactttggg atgcggttct tatggtaaga actctgtttc tcgtaacgtc tctgctgtaa
     1441 acttaatcaa tgtcaaaact gtagcgaaac ggagaaataa catgcaatgg ttcaaattac
     1501 cgccaaaaat cttctttgaa aagaactctc tattgtactt agagaaaatg gaaaacgttg
     1561 aacgtgtcat gatcgtttgt gaccctggta tggtccaatt cggttactgc gatacagttc
     1621 gtgaagtatt ggcacgacgt aaaaatgatg tgaagatcga agtcttctca gatgttgaac
     1681 cgaacccatc gacaaatacc gtatacgcag gaacgaaact gatggcagac ttcaagccag
     1741 atacagtaat cgctcttgga ggcggttctg caatggacgc agctaaggga atgtggatgt
     1801 tctatgaaca cccagacaca tctttctttg gtgcaaaaca aaaattcttg gatatccgta
     1861 aacggacata taagatcgac aaaccagaaa aaacacaatt cgtatgtatc ccgacaacat
     1921 cagggactgg ttctgaagta acgccatttg cggtcatcac tgacagtgaa acacatgtga
     1981 aatatccgtt agcagattat gcattgacgc cagatgtcgc aatcgtcgat cctcaattcg
     2041 tcatgtcagt tccagcatct gtcacagcag ataccgggat ggatgtgtta acacatgcaa
     2101 tcgaatccta tgtttcagtc atggcttcag attatacacg tggattgagc ctacaagcaa
     2161 tcaagttagt ctttgactat ttagaaaaat cagtaaaaac acctgatatg gaatcacgtg
     2221 aaaaaatgca caacgcttct acaatggctg gtatggcgtt tgctaacgca ttcctaggta
     2281 tttgtcactc agtcgcacat aaaatcggcg gggaatacgg gatccctcat ggacggacaa
     2341 atgcgattct tttaccgcat attatccgct ataatgcaaa agatccacaa aaacatgcga
     2401 tgttccctaa atatgactat ttccgtgcag atacagatta cgcagatatc gcaaaattct
     2461 taggtctgaa aggcgaaaca acagaggaat tagtcgaagc tttagcaacg gctgtttatg
     2521 aattaggaaa atctgtcggc atcaacatga gtctaaaagc tcaaggagtc acacaagaaa
     2581 cactagatac aaccgtagac cgtatggctg aattagctta cgaagaccaa tgtacgacag
     2641 ctaatccgaa agaaccattg atctctgaat tgaaacagat catcattgat gcttacaacg
     2701 gataagcaaa agaaagcgta gaaaaaagat aagaaaatag atgcaaaaga acttggaaaa
     2761 cgttgtttct ccaagttctt tttttaattt ccaagtaact gaaagttact tttgttattt
     2821 aacatttagg tcaattttag acagtttttg ttcaaaaagc gatatttata gtggacggta
     2881 ttactgctat attaaggata taaaatgaaa gcgtttaaaa aggtggcgta aagatgatta
     2941 ttaaagaaga gatttttgta cctaaagaat tagtgaaaga aaaaattgac ctgctcgttg
     3001 aaaacctgac aacgatcaaa gacgataatg gtgaattttt gttggacttt gatggactga
     3061 aagtagatga caaaagctgg acagtgtgga attggccaca aggtgtcggt ctatatggta
     3121 tttataaaaa ttaccagatg acaaaaaatc ctcgcgctta tcaagtggtc aatgaatggt
     3181 ttgaggatcg tatggaagaa ggcgctccgc ccaaaaacgt caatacaatg gcaccgctat
     3241 tgactatggc ttacttgtat gaagacacga aagattcacg ctatttgcct tatttagaac
     3301 aatgggcaga atgggtcatg aatgacatgc ctcggacaaa tgaagacggg ttacagcatg
     3361 ccacatatgg accggaaaat aagaatcagt tgtgggacga tacattgatg atgacagtgc
     3421 ttcctttggc aaaaatcggg aaactgttga atcgcccaga atatttagaa gaagctcgtt
     3481 accagtttat gatccatatc aaatacttga tggataaacg aacaggattg tggtatcacg
     3541 gctggacgtt tgaaggaaat cataactatg ccgaagcatt ttgggctcgt ggaaactgct
     3601 ggttgacgat tgctattcca gagatcattg aaatcttaga attatcgaaa gaagatgcat
     3661 tgcgcaaatt attgatcgaa acacttgaag cacaagtgcg tgcattaaaa acttatcagt
     3721 ctgaaagcgg tttatggcat acgttgttgg atgatccttc ttcttacgta gaatcttctg
     3781 caactgcagg atttgcttac ggtattttga aagcggtcca taaacgttat ttaccacaag
     3841 aatataaaga agtcgcttat cgtgctatcc aaggattgct tgaacaaatc gatgaaaaag
     3901 gagaagttca aaacgtttca atcgggacag gaatggggga cagtttagat ttttatcgga
     3961 atatcggaat cacagcaatg ccttatggac agtcgttaac ggtgctttgt ttgacagaat
     4021 tgctcgtctc ttattgttaa aagaaaggaa acccatatga aaaaaacatc actgctgttt
     4081 gcagcaagtt cgcttagcct aggtctgcta ggtctaagcg gctgcggaaa taattctagt
     4141 gtggcaactg tcgcagatgc agatgtaaat gagatagtta ccttgcgctt tgcttatgca
     4201 agcaacagcc agccagtaat tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa
     4261 acagatggtt ccgtacagat ccagtatttc ccagatggac aactgggtgg agaacgtgaa
     4321 ctgatcgaat tgacacagtc aggagcagta gatttcacaa aagtaagtgc ctcagcgttg
     4381 gaaagtttct ctaaagatta ttcgattttt tcagttcctt atctattcaa tgatgaagaa
     4441 catttcttta aggtgatgga taatgaagaa ataatggagc caatctacca atcgaccaaa
     4501 aatttaggat ttaccggatt gacctattat gattctggtc agcgcagctt ttatatggta
     4561 gacggtccga tccatacgcc ggaagatttg aagggaaaga agatccgtgt gatgcaaagc
     4621 gaaaccgcta tcaaaatggt agagctttta ggcggttcgc ccgtacctat gggaagtgat
     4681 gaagtgtaca cgtctcttca atccaatttg atcaacggat cagaaaacaa tgaatttgtc
     4741 ttgtatacag ctggacatgg tggtgtggct aaatactatt cctatgatga gcatacacgt
     4801 gtgccagata tcatcatcat gaatgatgcc atcaaagaac agctaactga tgagcaggaa
     4861 aaagccattg aagaagcagc gaaagaatca acggtttttg aaattgaagc ttttgcaaaa
     4921 gcaattgaag aagaaaaagc tttagcacaa gaagaacatg gagtccaatt caatgatgta
     4981 gataatacac cgtttcgaga ggcagttgcc ccacttcatg atatattcaa aaacgatcct
     5041 acctacaaag acctttatca aaagatccaa gagcaaggag cgtagctgtg atgaaaatga
     5101 ttttaaacaa ggtattagag atcctgggag ctgttttgct tgttgggatg gtcgctgttg
     5161 ttttatggca agtattttcc agaacggttt taggaaatcc aaatacggtc acggaagagc
     5221 ttgttcgatt cggtcttgtc tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa
     5281 aagcacattt agccgtaacg gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt
     5341 tagaagttgt tgttcaaagt ttatttctgg tatttgctgc gatgatcatg gtgtatggcg
     5401 gatggaatgc tgtcacattg acaatggggc agatctcgcc ttctttaagt attcctatgg
     5461 gatatgttta tctgtcagta cctgtatcgt gggtactcat cattatctat agcctgatca
     5521 atctaatcga tacattaagc aacaagccag caattcaaga aaaaatataa aaatggagga
     5581 ataaaatggc aatagaagcc ggattagtgt tattttttgt atttgttgtg ttgcttgtat
     5641 tagggatgcc gattgctata agtattgcat tctcatcgat gatgacttta ttgttagtta
     5701 taccgtttga tgtttctgct tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt
     5761 tttcgctgat cgctattccg tttttcgttc tctcgggcat tatcatgaat aatggaggga
     5821 tagctaagaa actagtggat tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg
     5881 ctcatacgaa tgtgttaggg aatgctttat ttggttccat ctctagctcg gccattgccg
     5941 cttctaccgc gattggcgga gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga
     6001 agtttgcgac agcggtcaat atcgcgtcag ctccgacagg tatggtcatt cctccaagta
     6061 ctgcttttat catgtattca cttgttgctg gtggtgcttc gatctctgct ttgtttatgg
     6121 gaggttatct cgtaggcgct ttgtgggcgc taggaatcat gctagtagca ttcatcgttg
     6181 ctaaaaagaa taaataccct gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa
     6241 taaaagaggc gattcctagc cttctattga ttgtcatcat tatcggtggt atcttgacgg
     6301 ggatatttac agcagtagaa gcttcagcga tagctgtcat ttactccctg ttgattgcta
     6361 tgttttacta taaaacggta aaactaaaag atatacctaa tatgttgttt caatcagtag
     6421 caatgtcagg gacgatcatg ttcttattgg ctacttcttc catgatgtct ttcgccatgg
     6481 cgtttaccgg tattcctcag gcaattagtt cgttgattat gggcgtaacg gataataaat
     6541 ttattattct gttattagtc aatcttgtgt tattactggt gggtatgttt atggatgtgg
     6601 cgcctgctat cttgattttt acaccaatat ttttaccaat cgtcacaagt gtaggggttg
     6661 atcctgtaca ttacgggttg ttttcaatca tgaatctgtg tgtaggctcc atcacgccgc
     6721 cagtaggtac aggactatat gtcggtgcaa gtgtaggagg cgtaaaagca gaggcaatgc
     6781 tgaaaccgct tcttcctttt tatggggtta tcttagtcgt cttgtttttg attacttact
     6841 tcccgcaaat cgtcatgtgg ctacccaata tgctgagtta ggagaatagt tatgttgaac
     6901 attacaatta gaggacatga tctttccgaa gtccaaacga tagaagatct tgcagaaaaa
     6961 acaaaagaac aagggattca tacactgcag ctcgccttag gtctttcgtt tcctgaaata
     7021 ccttctggcg caaatgagat caattctggg atgggaaatt atgtcaaacg agttttagaa
     7081 aagcaagaag tgaccgtagg aattttaagt tgctatatca atatgattca tccagattta
     7141 acgatacgac aagagttgct gacaaagttc gagcagtatc ttcgctatgc ttcttccttt
     7201 ggtgcgtcga tggttgcttc ggaaactgga tgtgttttgc cggaaatcca atatacggaa
     7261 gaaaatttca cagatgaagc atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa
     7321 gctggagaaa aataccaaat gatggtgggg atagaaccag gtttgaatca tccggtgtat
     7381 tctttagcaa gggtagagca gctgatccaa gcagtagatt cggactatct aggaatcatt
     7441 ttagatccga ccaatttgat cacaagtagt gattatcaag aacaagtcca attggtagaa
     7501 gaagcttttg aacgttttgg tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc
     7561 gaacaggaaa aaatcgtgcc ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc
     7621 aaagaaatca tcatgaagaa tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat
     7681 gatgggattc gttatggaag aagtcttctt gaataatgta aagaagacgt tggttaacta
     7741 atatttagaa tggagggggc ggggctagaa gatgccacgt ctcttctttt ttataagagt
     7801 atataaaagc agctcttttt gattacaaaa aacgtggtat aataaaattg tgcataagtg
     7861 aacaaaagga ggtgcggcac atgctccaaa agaaaatcag cggtgaacaa gtcaatacga
     7921 tggtttctga tcagtatgaa atctatcatg tcaaagatgt cgtcagccaa aataaaacga
     7981 tctatcatca tcatgatttc tatgagatcc atgctacatt aaagggagaa gcgacttttt
     8041 atttgaacgg acgacaattt acgatcaaag caggaagtgt gctgctcatc cattccaatg
     8101 atttgcatcg tatcgtccgc caatcttcag atgtattcga gcgtgtctat atctttgtca
     8161 ccccttcttt tctagaaagt cgttccacgc catggtctaa tctatcggct tgttttcaac
     8221 caatgggaga gaagaggagt cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat
     8281 tatcatttat cgaccatcct cctgataaaa aaatttacgg tgaagacata aaatatgaac
     8341 aagctctggt agaatacttg atttttctca atcaagttgt acaaaaagaa gagagtgcgc
     8401 aagaacagaa tgctgtagtt cctaatgagc gtgtggaaca aatgttacaa tatatctcaa
     8461 aaaatttatc tcatccgctt tctttagagg aaatggaaaa gcagtttttt gtcagcaaat
     8521 accatatcac acgagaattt aaaaagtaca ctggatttac gtttcatcag tatgtaatga
     8581 agaaaaagct gatctatgcc aagcacttgt tgcgagaata ccgcagctct agtgctgtat
     8641 atagtaaaag tggtttttct tcttatccgc attttctgaa agcattcaag agagagttcg
     8701 gcataacgcc taaagaattt ttgaaacgtg atacacagaa acagttggtt cattatgaac
     8761 attttgaaga tgaacaatga gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt
     8821 tacatag
//
LOCUS       DAJSMI010000109         8749 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJSMI010000109 DAJSMI010000000
VERSION     DAJSMI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.110"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..267
                     /locus_tag="ON430_002015"
     CDS             <1..267
                     /locus_tag="ON430_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219925.1"
                     /translation="LKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRTRSAKGIYE
                     MKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(364..798)
                     /locus_tag="ON430_002016"
     CDS             complement(364..798)
                     /locus_tag="ON430_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3219926.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1030..1740)
                     /locus_tag="ON430_002017"
     CDS             complement(1030..1740)
                     /locus_tag="ON430_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR3219927.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1730..2590)
                     /locus_tag="ON430_002018"
     CDS             complement(1730..2590)
                     /locus_tag="ON430_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCR3219928.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2595..3233)
                     /locus_tag="ON430_002019"
     CDS             complement(2595..3233)
                     /locus_tag="ON430_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR3219929.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3248..3547)
                     /locus_tag="ON430_002020"
     CDS             complement(3248..3547)
                     /locus_tag="ON430_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3219930.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3590..5074)
                     /locus_tag="ON430_002021"
     CDS             complement(3590..5074)
                     /locus_tag="ON430_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR3219931.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5094..5555)
                     /locus_tag="ON430_002022"
     CDS             complement(5094..5555)
                     /locus_tag="ON430_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3219932.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5573..6640)
                     /gene="ulaG"
                     /locus_tag="ON430_002023"
     CDS             complement(5573..6640)
                     /gene="ulaG"
                     /locus_tag="ON430_002023"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR3219933.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6858..7628
                     /locus_tag="ON430_002024"
     CDS             6858..7628
                     /locus_tag="ON430_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3219934.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7901..8728)
                     /locus_tag="ON430_002025"
     CDS             complement(7901..8728)
                     /locus_tag="ON430_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3219935.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2644 a         1850 c         1375 g         2880 t
ORIGIN      
        1 cttaagcaat ttccacaaga agaacatttc atgtataaag aagcaatttc ccacaaagtg
       61 acagagtatt tggagcataa tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa
      121 ggaatatacg agatgaaagt caaaattaat aaaaaaaatt atcgcatagc atttgcttta
      181 aaaaaaaatc atgtgttcat tttttatatt agttcgaatt tgcaaaaaat tgtatttgat
      241 aaagaggtga gtaaaaaaat aaaatagctg agcattggag gatagattga gtgatactaa
      301 gtctatcctc ttgtttttct aaagaactaa gctagaattt tttcttttgt acgttattat
      361 atgttatttg tcattaccaa aaagcacctt ttttcttcta gtatctatga cataagaaat
      421 accacccatt tcctttactt gaacaaatcc tactgattca taaaattttt ttgttttctc
      481 agtatcatct gtcaataatt cgatttggac tggtccataa ctagtgatct tctccaaagc
      541 ttcattcaaa agcactttgc ctattccttg gcgctgatac ctcggatgaa tgaggatatc
      601 ttgaataaat gcgatcgtcg caaaatcagt gatccaacga acgattccga taatcttccc
      661 ttccccatta cgctttatca gagattcaga ttgttcaaat gctctagcta accttgcagt
      721 gtcttttgtg taattcgtcc aaccaacgct cttatataat tcagaaatct catcgatcat
      781 aagggaatca atattcattt ttttcttcct ttcttataca attctccctc taataataca
      841 gttctttgac tatttaatct atcacatata actctcctgt aaagattctc tcgattcctt
      901 aaaattcctc tttcctttta gaatgcatac tttttgataa ataaaaaggt tgtgataaat
      961 tttcttcaca acctctgttt ctctctatca agtgttatct tttaattttc tacccttacc
     1021 ctagcatctc tattgcccgt agtatgcata ctttccatgt ttgcgaaaat aatgtttatc
     1081 tagtaaatat tgcgggattt cacatatatt ggggttgatc agctcgtttt ctttggccat
     1141 cagacaaatt tcatctaaga tcaaactgtt ttctactgct ttcataggtg aatcccccca
     1201 tgtgaacggt ccatgtccat agacaagtac tccagggact tcgtttggat ctaattttct
     1261 ttccttaaac gtctcgacaa tcacattccc tgtatgaact tcgtaagctt ctcttacttc
     1321 ttctttcgtt aattggcgtg tacaaggaac cttaccataa aaagcatcag catgagtcgt
     1381 gccataagct ggcaaatcgc gtcctgcttg cgcccacatg acggagtgcg tggaatgagt
     1441 atgagtaata gcagttatat cttcaaaagt ttgatacaaa acaacatgtg tggggagatc
     1501 tgaagatggt tttaagctat cttcttctat tatatttccc gacaaatctg ttaccaccat
     1561 ttgatctgct tgcatgcatt catatctgac accgcttggc ttaatgacga tgactcctag
     1621 ctcacggtcc acttcactca cattgcccca agtcaatttg actaatccgt atcgcggcaa
     1681 tgccagattt gcttcaagga cacgttgctt cattgctgca attttttctt cacaagacat
     1741 catagccagc ctccttcaat ttagaactta agtagtcttt tgcctgctgg atttctcttc
     1801 gaaagtcttg gcttttttca ctccacattt caatcagaaa tgttccatca taatctagac
     1861 gtttcaaatg tcttaataat ccaagaaaat caacacaacc gctaccaaaa ggaacatcac
     1921 gaaactggcc tgaatattct tttgttacag ggtaagtatc ttttaagtgg atagcagtga
     1981 tataatcgat ccctttctca agttctcgag ccggattatt ttccggccat gcggacaaat
     2041 tgcctaggtc tggatatact tgtagaaaag gtgaatgaat ctgttctttg atttctaaaa
     2101 attttgaaat ggagttcata aatggatcat ccatgatttc cacggaaaga atgatgccat
     2161 atttagaagc ttcctttacg ctttctttta ttccttcgat aaagtcttct ctggaattca
     2221 ttgatttctc ttcataataa acatcgtatc cagctatttg gattactttg acgcttagat
     2281 tatgcgccaa atggattgct tttttcatta gttgtttcgc tgcttttttc ttttctggat
     2341 cagcagaacc aaacggaaat cgccgatgcg cacttaaaca aatcgaatgg atacgtacac
     2401 cggagctgaa catctcttct ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg
     2461 ctaaccgctc atccgtctca tcaatcgaca tctcgataaa atcgaagtcc atttctttta
     2521 caagggaaaa acgttctttc catgaaatat ctttgggtaa cgctttttca taaatcccaa
     2581 tttgagccat ttccttaccc ccagatacga atgatctcat ctttgaagtt ttgtgcggcc
     2641 ttcatcggat catttgctgc tgtaatacca cgaccagtga taaaggtata cacatcgatc
     2701 ccttcaaata agtgtaatgt gtgtggattt aatccaccag ttacagaaac attaaagcct
     2761 agttcaatca gttttttgat cttcgataaa tctttttcac cccaagtttc accagataac
     2821 agcgcatcac ggctttgatg gtagattacc tgccggatac cgatgttatg ccaatccatg
     2881 gcttgttcaa aagtccaatt gccgtagagt tctacttgca attcacctat ttctttttgt
     2941 gctgcctcca tagttggaat cgttgcacaa caaatgactg tcatccagtc tgctcctgct
     3001 tttgcaacat tactcgccac tgttcctcca gcgtctgcac atttcgtatc agcgactagt
     3061 tttttatctg ggaacatact gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa
     3121 agaattgtgc ccacttcaat aatatctact acatcaccaa ctttcataac atctcctaat
     3181 gcatgttcta agtcattgtg atctaacgca atttgcaaat tcggcctgct catcttagtt
     3241 ccttccttta cttgtttttt tggtaccact ttgtttcagc aaatttttca ctgatttctt
     3301 tgactgacat cacatttttg ataccgataa ctgtaactcc ttttttctct gcctgagtaa
     3361 acatattcaa aaaattgact ggcgtaaaaa ctacatcgta ttggccagct gaactttttc
     3421 cctctgaaat ggcacaatga tggattttgg taattggtac gtttagttct ttcattgctt
     3481 tttccacact tctcatcatc atcaagcttg ttcctgatcc atttgcacac gatactaaaa
     3541 ttctcatctt aatccctcca ttatgagtgt tgtctaatca gattttcttt tatactctct
     3601 gcttttctgc atatgtgtca tagtcttcta caatcaaaaa ataaccatca ggatttttcc
     3661 gatattgtaa ctgcggaata gccaataaac caatgacaac tacggcaatt ccagcataac
     3721 ctaggacttt catgattact gtaaacaatg gccaagctgt tgcccagtcg aacatcccga
     3781 tataaccacc gtatgttgac atcccaatcc atgtagcgaa taatgctgaa ccgcctactt
     3841 gtatgattcc tgaaagaaac gggaacaggc aagcggcttt gaatccacct cggttattgg
     3901 cataaactgc tataacggca ttatcaaaga acaacggaat gaaaccggct atgacgatcg
     3961 ttggtgattt caataaaatc agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca
     4021 agaacccaat cgttaccgca tttggcgaac caaagccaaa agtagcagct acgtcaattc
     4081 ctggaacagc tcctggtaaa atcgtgtttg agattccttg gaaagattga gttagttcat
     4141 cgacgaatgt ccgaacacct aattgcaaga tcgccagata taccgcaaaa tttaaagaag
     4201 tcgtcataat gtagaataag aaactctgcc cttcttgcat gaattgcgct tgaatcaaat
     4261 aatctttgcc tagtacaatc agaatgatcc caaagaaaaa tagcatgagt aacgaagtag
     4321 caaccatatt ctcattgaaa atcgacaaga atcctggtaa tttgatatct tcaatttttt
     4381 tgtcttgctt gattgatcta tttttctttt catcccgctt tttcatccac tcagctaatt
     4441 ttgcaaatat aaagattccg aacatttgct gatgtgccac tgcaaatccg gcaccttctg
     4501 tcaattcctg cgtgattcct actgttaagt tagaagctac tgcccaataa caacctaaaa
     4561 tcagtcccat aaaaatcaat acttgtatcc tacctagatc aggaaagcaa aacagtaaaa
     4621 tccaaaaagc ggtagctgct tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca
     4681 gtttcgtata tttctgaaaa cggactaata agatatttaa aacaaatgca atcagtaata
     4741 aaatcatcac atctccaaag gtacggccaa atgtctccat taatccagca tccacagcat
     4801 tttgtccaaa ataaggatct gtaaccatgg catccaaatt gaaccgttcc tttagtccaa
     4861 ccaagattgg ccgaaaatta ttgaccaaac caccagagcc aacagtcaaa ataaagtaac
     4921 cgaccgttgc ctttaagaat ccagcgatgc attcatgaag cgggcgtttc agcaaaatat
     4981 agcctaataa cacgataaaa ccaatcaggt atgctggctg cgtcaagata tttgttgcaa
     5041 aatacgtcca aatgcttaac aaaaattccc ccatcttttt tctcctctat caattaatta
     5101 tattctgcca tcacaacttg gtaatcatcg atggagttcg tagagatgag acgctcaacc
     5161 aatccatctg tcattaataa ttcggataat ttttggatat tttctagatg ttcttctgga
     5221 tcttttgctg cgagtgtaaa aaacaaacta gccgttttct cctcattctc cccttcactt
     5281 tcaaaacgga ttggctgttt caacttggta aatgaaatag ctgttccaaa aacaccatca
     5341 cttttttctg aagaatgcgg cattgctact tgaggtgcga tcacaatata aggaccgtat
     5401 ttttctacac aatggatgat ctcctccaca tacgtttcat caataatttc cttatcgatc
     5461 aatacaagac agctttcggc gatagcttca cgccaatccg tgatttcttt atctgggtaa
     5521 tgaatcaatt gatggtcaaa aaagtatttc agcaagaaac tccccttctt tcttataaaa
     5581 acgacggaaa cggtgtatca ttttcaatcg agaagacatc ttcaaatcct cgataaaaat
     5641 taaactcaag atcatctttg tttgtcggat agatgaattt cccgcctact tgccagatat
     5701 aaggtttaaa tccatacttc aggcgatcct ttttgaattt ccaaatttca gtgatctctt
     5761 ttggatcagc cataaaatta gaccaaatat cgtagtggac aggaatcact acttttgcat
     5821 tcaatgactc cgccatgcgc agcatatcaa cagaagtaac tttgtctgtt atccctcgtg
     5881 ggttttcacc ataggcccct aaacagacat cgattttgtg ttcattacca tgtttggcaa
     5941 ataaattgga gtaatgtgaa tctcctgcat gataaagatt ccctcctgat gtttcgaata
     6001 aatagtttac tgcaatttga tccatctcct gaggcatttt tccttttaat acctcttctg
     6061 gatcttcaca agtgaccaac gctgtccgat caaacgcttc taaagctact acattgatgt
     6121 ctttgatttt gactgcatca cctggcttta ctgtaatcgt tttttcttca ggaattcccc
     6181 atttttgcca cgtgcgaacc acttcttttg gcccaatgaa tgttgcttca ggacaatttt
     6241 gatgaactgc tgcggctgta tggatatcta gatgatcgga atggatatgt gtaacgatta
     6301 aagcgtctac attttttatc gcaaaaggat cgattacgaa aggctgagta cgtaaattag
     6361 gctgcatatt ttgaacgcca ctcatacgca tcatctgatg gccttttttc atcatgccat
     6421 tcccatgact acgttttccc gtcccacacc atagatcaca taaaatattc gttccttcat
     6481 gacttttcag ccaaattccc gtacagccca gccaccacat agcaaaagtt ccaggatcca
     6541 ctacttctgt ttcgatttct tcattcaagt atgtgcccca ctcaggaaat gtttctacta
     6601 accacttttc ttttgttact tcattgacag ttttactcat ttgcttgctc cccccatatc
     6661 gttgttccaa cttacaagta aatattaatg tgtatcagca tccatgaaaa cccttttata
     6721 tgaccgtttg ttttttatat gattttcttt cagtaatcaa tgattgtttc agtataataa
     6781 agacagatat gaatgtttaa atgaacaact agtcaaataa atccatggat caagatgtcc
     6841 ctcaggaggc aaaagtcatg aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa
     6901 ttttggaact gcttcagcag actgaacaac tatcaacttt cgaattagct gaatcccttg
     6961 atgtatctat tagtacgata cgaagagatt taaatttatt agaagaaaaa aacgagatca
     7021 tacgcaatta cggttattgt tcttataatt acaaaaacca gactgatttt gatcaatctg
     7081 gtcctgaaag gattaaacaa gcgattgcca gagaagctag taaatacatt aacgattatg
     7141 atacgctgtt tatcaattct agctcgactg cgttgaaaac actggatttt cttcaatcta
     7201 accatttgac agtcattaca aacaacgtga aaatcaatta ctctccgcac aaagataatt
     7261 gtagttatat tttaactggt ggagagatgc gctttccaaa agaagcacta gtgggcgaca
     7321 tcgcattaaa tacgatttct tcagtcaatg cagacatttg tatcattggc tgtagcggag
     7381 tcgatgtagt acatggggtc acaacaaaga tattaaatga atcaacgatc aacgaaatga
     7441 tggtcaacaa gacaatcaaa tgtaagattt tagtcgctga tcatcgaaaa atcggactga
     7501 catcaaagtt caagattgct gatatttcag tctttgatta tttgatcact gataaatttt
     7561 gttctgaaaa agtattagag gagatcagaa atacggggat aaaaaccatt caagtagatt
     7621 cattttaaga accaggacgt aaatatgtaa ataggattat caaaaaaatg aataacaaat
     7681 ttatcgataa atgaaaaaca cgctacgctc aaattgataa aggaatccca agatgtatcc
     7741 atccaatcga taccatcata atctattgct tcttttataa ccgttatatg ttgcctaaat
     7801 aggaatcaaa caatatcgaa aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa
     7861 taaacggtat tcaaatcaga acactgattt cacaaccact ttatttcagg tcgttttcaa
     7921 tcacctttac cgcgtaatca tttccgttga aacgcgctat tttttttgcg tccatatatc
     7981 tttctaaata atcatcttct cctaattgct tatggcaaga agcaatttca taaagcaagt
     8041 attccaatgc ataagtcgat ttatgtttct tattatactc aatacccttc tcacataatt
     8101 ggattgcttt atcataatcc tcgattttac ggtaataggt ggcgatatca taatatacaa
     8161 tgcagaccat cagattatca aaatcagtct cactcaatat tttaagtgta cgctccacat
     8221 aaactttcgc aaaatctaat tctccttttg acaagtagat cattgccacg tttgcagctg
     8281 cttgtaagtg atggatatct gtttcggtcg tttgttccaa cacttgatta aaataaaata
     8341 atgctgcatc tggttttttc gcataaagat gatcaagcag tccttgataa aataaaattt
     8401 gatttaaaag atcagaatta cttacttttt cctgatcgat cgtttgtaaa attttataag
     8461 attcttggta ttttgataca gaattcaatg attgaatctc atttattttt ttcctgattt
     8521 tgtcttcttc tgtttctact aatatatcgg agatctcgac attcagcttt ttgcaaagcg
     8581 cacttaagat attgatgcct ggcgacatat tcttgttttc gatattgctg acagttgctt
     8641 gttcacaaat gccttctgct aattgtactt gtgttaaccc ctttttcttt cggtattctt
     8701 ttagtaatgc tccatctact ttaatcaaaa caatttcctt ccctttaaa
//
LOCUS       DAJSMI010000110         8720 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJSMI010000110 DAJSMI010000000
VERSION     DAJSMI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.111"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(135..2516)
                     /locus_tag="ON430_002026"
     CDS             complement(135..2516)
                     /locus_tag="ON430_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR3219936.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLTRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2506..3153)
                     /gene="recU"
                     /locus_tag="ON430_002027"
     CDS             complement(2506..3153)
                     /gene="recU"
                     /locus_tag="ON430_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCR3219937.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3217..3756
                     /locus_tag="ON430_002028"
     CDS             3217..3756
                     /locus_tag="ON430_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCR3219938.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3846..4265
                     /gene="gpsB"
                     /locus_tag="ON430_002029"
     CDS             3846..4265
                     /gene="gpsB"
                     /locus_tag="ON430_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCR3219939.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4287..4654
                     /gene="rnpB"
                     /locus_tag="ON430_002030"
     ncRNA           4287..4654
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ON430_002030"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4863..6029
                     /locus_tag="ON430_002031"
     CDS             4863..6029
                     /locus_tag="ON430_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCR3219940.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6075..7571
                     /locus_tag="ON430_002032"
     CDS             6075..7571
                     /locus_tag="ON430_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCR3219941.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7652..8335
                     /locus_tag="ON430_002033"
     CDS             7652..8335
                     /locus_tag="ON430_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3219942.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2742 a         1581 c         1847 g         2550 t
ORIGIN      
        1 agatcacaca tgtatatgct ggatagtctt ataaaaaatc atgtaataca gcttggtaca
       61 tgatttttat ttgtagctgt tttttacata gacacaaaaa agaaaccacg cttgtagacg
      121 tggtttcttt tttactatct gtttccagaa gctgaggtgc tgctggatgg tgtatggtca
      181 tttgcagcat ccgctccgct agttgctggc tcactggaag aagcaggagg agcagatgaa
      241 gacggtgtcg agctttcttc ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg
      301 cttgtttctg ctgtcgtgct ttcttttgag gactcagcag ttgattcgct ttgactagta
      361 gagctagagc tttgagtcgt tgtctctgtt gttgagcttc caggagtctg gacatagctg
      421 gaagatggaa tagttgttga tggtgtaatt gcattcgatc tagctgtata ttgatctttg
      481 tagtacagtt caccgcccac acgaattaat ccgcttggca tctcccagtc tgtatttgtc
      541 acgttagctg aaacatactg cataagctcg cgataaacat cagaggctac gtgtgacgat
      601 tctgaagtga caggtgtcat ttttttattg taacctgtcc acacggaaat cgaataattt
      661 ggcgtatagc ccgcaaatag gatatctgga tacaccccag aagaaatacc aagttttgca
      721 tactcgtcat ctgtgtaatt ggatgttccg gtcttaccgg cttgatataa cccagcgatt
      781 tgcgcattcg taccagttcc ttcagtgatc gtatctttta aaatgtctgt gatcatataa
      841 gctgtttctg gagacatggc tgtttttcca tctggctcat aagtttcttc tgttccgtct
      901 tggaagacga ttttattaac atattgaggt ttgtaataag tccctccatt ggcgaaagcc
      961 gcataggctg ctgccatttt taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc
     1021 gtattactag agatcgcatt cgattgatgg atcgtactgt attcgatccc aagatttttt
     1081 aagaaagaag ccactttatc tgaaccgact tcattgaata atttgactgc tggaacatta
     1141 cgggttaagt aaagtgcttg gcgtaaagta atggtgccca tatattgatt atcccagttt
     1201 cctacaggag tcgaagtccc ttcatagtta tacggtgcgt cagtaatagt ttttccagta
     1261 gaatatttca aatattcaaa agctggtccg taatctgtga caggtttcat cgtagaacca
     1321 aaatcacggg aagtattcac tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga
     1381 cgtccgccaa tctgggcttt tactttcccg gtattggtgt cgataagggt ggaggcaact
     1441 tgcatctcgt cgtctgggta agagacatac tgatccgtgt tgacgatatc atatagtttc
     1501 ttttgggcgt ccagatccaa attcgtataa atctctaaac cgtctgtgta gacattctta
     1561 tctgtttttt cttgtacttc attgatgact tctttgacat aattatcgac gatcttagta
     1621 ttgtcatcgg attgagttag ttcttgcaga ccatccgtta ccggtacatt aacagcttga
     1681 tcatattctg tttgagagat tttttcgttt tgaagcatcg tatacaaaac agtatctcgt
     1741 cttttcttcg cctgatctgg ataaacataa ggatcatagg cggaaggggc ttgaggcatc
     1801 ccagcaagca atgctgtttg cggtaaagaa agttctgata atttcttacc aaagtacatc
     1861 tcagaagctg tctccatccc gtacaagccg tttgacatat acactttatt tacgtagtag
     1921 gtcaagatct cttgtttcga ttttttttgt tccaatctta cagccatcca cgcttcttgg
     1981 gccttgcgtt tcagagtctg atcctcggca ctggtggaga agaaggaaag tttgatcaat
     2041 tgctgtgtca aagtacttcc gccttgcagt ccgccagagg taaagttaga gagggcagaa
     2101 ccgatgatcc gaatagggtc aaccccgatg tgtttataaa aacggcgatc ttctaccgac
     2161 acgatcgcat cttctaatgt tttcggtaat tcattcgcag aaattttttc acgcttttct
     2221 gcgcctaaat cttcgaatag ttcgccatct tgtgtatata gtttggaaga tacggtagcg
     2281 tctaattttt tatccgtcaa ttcgggcgca tcttttgcgt aataccagaa aagtcctaca
     2341 cctgctaaaa agaggataca gaaaaatgag agtattccta aaaggatctt gataaataat
     2401 ccttttttat gtgttccaga ggactttcca gaacctttgc cagaattctt cttcacactc
     2461 tttttaggag taggctgttt ctgtcgtcta gagcttctag tttgttcatt tgccattctt
     2521 gctttctcct tcaatagata atgttgataa atagaagtca accgcgtcca aataaggaat
     2581 ccgcggagaa ataccagttt tgatctctat tcctagttct tcgatcaaag ataaaggtag
     2641 tgatttgcgt cctttttcct tttgggcgtc ccagtaagca atcaaatgaa cactattcaa
     2701 ataaaaacag cgcttcaaag acgaaaacca taacaagaca aagcagaccc cattttgttc
     2761 gaggcatgct ttcatatgtt cgatttgatg ggcatggaag tttttaaagg gaaaagatgt
     2821 tttgttcttg gtttcttttg cttcaaagtc caagtaaaag cctcgataga cgccattata
     2881 gtctgtagtt gaggcttggg taaagtaggc ttccttgatc acagcagcac ttctcttagg
     2941 atagtctact ttcacgattt ggataggggt aggcttttta tggataaccg ccaacccgcg
     3001 ggaaagataa tatttattac tttcattgat tgcttcttca aagcgcatcc cccgattgcc
     3061 gaattcagtg atctgatttc ttttttcttg aattttttcg acgggagagg cattcttata
     3121 ataaggttgg ccatttggat agcgcaatac catctttaat cacactccta ggccacatta
     3181 tagcaaaaaa gcggatagaa agaaaaggaa tctgagatgg ataagtcaaa agtaatctat
     3241 gtgtctgggt atcgaagttt cgaactcggt gtttttaaag aaaatgatcc taaaatccaa
     3301 gttgttaaaa atgttttaaa aaaagaactc catcagttag cagaagaagg gctggaatgg
     3361 gtacttgtca gtgggaattt gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg
     3421 aaagaagaat atccggaact atctcttggt attttgtatc ctttttctga ttttggtaat
     3481 aactggaatg aagggaacca agaaaaaaaa caattagcag aacaattagc agattatgtc
     3541 gaagctgtta gccatcaacc ctaccaatca cctagtcagc taaagaatca cactcgtttt
     3601 atacttgagc atacagtggg ctgtctgctg atctatgatg aagaattccc tggaaaaacg
     3661 aagtattttt ttgaagatgc acagaattac caagcagatc atccctacga aattcggctg
     3721 atcagtatgg acgatcttca aaattttagt gaataatcga tattgtgttt gatttatcaa
     3781 cttttttcct atacaatgaa aaaggtagaa atgttattac gtaaatgaaa actagaggtg
     3841 taaatatggc gaatttggtt tatagtccta aagacatttt acaacaagaa ttcaaaacaa
     3901 aaatgcgcgg ctatgatccc gttgaagttg atgaattctt agacaatatc atcaaagatt
     3961 atgaaactta cagcaaagaa ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa
     4021 aagtagctca attatctaaa actcaaggag cagctcaaac acgcgtgcaa caaacagagg
     4081 caccaaagag cgcagcagtg accaattttg atatcttaaa acgcttgtca aatcttgaac
     4141 gtgaagtatt tggtaaaaag cttgatcaac aagcaagtgc agtcaaacca gcacaaccta
     4201 atccaaataa ctacacaaat gcagacacaa gcttagatga taatgaaaaa acacgtcaat
     4261 tttaattttt gattaatgaa tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc
     4321 tgaggaaagt ccatgctcgc acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc
     4381 ataagctaag gtactctttg gagtaacggc aggaaaacaa gctaaggctt cggctatgct
     4441 taagtatcct tgaaagtgcc acagtgacga agcggctggg gaaacccatc cggtggaacg
     4501 cggtaaaccc ctcgagcgag caacccaaac aatggtaggg gcgctttttg tctggaagaa
     4561 gaacaggaca aaaaggcagt ataactgcag atagatggtt accgctttgt tttttctccc
     4621 tggaaaagca aagtacagaa catggcttat agaaaaccgc aaacatcagg gaattaggtc
     4681 gtgacaaaag taatgcgtta taagaaacaa gaaaaacatt tgaaagctat ttttcaatac
     4741 ttcagatgaa tcgttcttat tacaaaacaa acttttgtat caccggttat tcggaatttg
     4801 agtaaaagtg tggggtagga ctgatgtccc acctcctttt tttataaata aggagattgt
     4861 gtatggaaaa aaacaggaag ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac
     4921 tggttggtaa agaattaaga gatcttggaa tcgaatgcca agttgagaac ggacgtgcac
     4981 gttttgaagg aacaatggaa acgatcgcta cagctaattt atggttacgt acagcagatc
     5041 gagtaaaaat cgtggttggg gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg
     5101 taaaagcatt gccttgggaa gattttcttc cactagatgc agcttttcct gtagctggaa
     5161 aatcgatcaa gtcgaaacta tatagcacac ctgattgcca agcgatcaca aaaaaagcaa
     5221 tcgttgaacg tctccgtacc tattatcacc gtccagcttc tgtaccactg acggaaactg
     5281 gcgcacattt tcagttggaa gtagctttgc tgaaagacca tgtgatggta actctggata
     5341 cgacaggacc aagtttattc aaacgtggtt atcgcttaga aaaaggtgga gcaccattga
     5401 aagaaaacat ggcagcagca cttattatgt tgacaaattg gcgaaaagac cgtccatttt
     5461 atgatcctgt atgtggttca ggtacgcttt gtattgaagc agccttaata ggccataata
     5521 tcgcaccagg attcaatcgt gagtttgctt gcgaatcttg ggattggttc agtcaagaaa
     5581 tcatggaaaa tgttcgatca gcagccgaag aaaaagcaga ttatgatatt gaattagata
     5641 ttactggttc agatatcaat ggacggatga ttgaaattgc caaagccaat gcagaagaaa
     5701 tcggattagg ctcttcgatt acctttaagc aacaagcagt caaagacttc aaaacggata
     5761 aagaatacgg ggtcatcgtg gctaatccgc cttacggaga acgtctagga gaagaagaaa
     5821 ctgttcggaa attatataaa gaaatgggcg atgtattccg tccattaaaa acatggagta
     5881 aatatatctt gactagcgac ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa
     5941 aaagaaaatt gtacaatggt gcattgcgta cggatctatt ccagtattgg ggaactcgtc
     6001 cgcctagaaa aaaagagaaa gacgaataat cttactaatt gattcattca attggatgtt
     6061 tggaggaata gggaatgaaa gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc
     6121 tacaacaagc tttaggaatc ctcgattggg atacacaaac aggtatgcct gaaaaagcta
     6181 gtgcataccg aagtgaagta gacagctatc tttacagcat ttatttttca aaaaaaatcg
     6241 gtccaaaaat caaagaagcc attcgctatt tttctgaaca tccggaagaa ttgtcggaag
     6301 ttggaactgc cgtttttgcc aaagtaaaag aagagtatga tttggaacat cgcgtaccgg
     6361 aagatcttat gagaaaatat acagctgccg tttcttcagc acacggacaa tggcaaaaag
     6421 ctagagaaac acaacaattt tctgattttc aacacgcatt gaatcaaaat attgaattaa
     6481 caaaacagtt gataccttac tggagaaaag atgaaaagac gaattatgat gttttgctga
     6541 atcaatacga acccgggatg actgtcgaaa tccttgataa ggtatttgat cagcttaaag
     6601 aaggaattct ttcgatccgc cgtacgctcc aagaaaaagg gactgaacct gaaacagact
     6661 ttttaaatcg gagaatgacg aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac
     6721 aacttggtta tgattttagt cgtggtcgtt tagatgacac tgtccaccca tttatgactg
     6781 gaattaatcc gaatgatgca cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg
     6841 ctgtttttgg agtgatccac gaggctggtc atggtatgta tgaacaagac atcgatgata
     6901 aatatgcgta tacaccggtt tatgaaggta catcaatggg tatccatgaa tcacaatcgt
     6961 tgtttaacga gatcattatt ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct
     7021 ttcaagaatg tgcagaagga acattcgatg atattccttt tgacaaattt tatcgttcat
     7081 tgaaccatac aaaatcaagt ttggttcgta tcgaggcaga cagtttgact taccctctcc
     7141 atatcatcat tcgttatgaa atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg
     7201 aactacctga aatatggaat caaaaatatg aagagtatct gggaatacga ccaacaaatg
     7261 atgtggaagg catcctacaa gacgttcatt ggtcaggtgc aagctttggt tattttccct
     7321 cttatgcatt agggtacatg tatgcggcac aattacttca ttctttgcag caagaaatag
     7381 atgtggaaaa tgttctagcg tcagacgatt attctccaat caaaaactgg atgtcagagc
     7441 atatccatca atatggtgca tctagaaaac caaatcaatt aatcatggat gcaacacatg
     7501 aaccgcttaa tccgcaatac ctgatcgact atatgaaaga cctgtatttt aatgtatatc
     7561 aggtaaaata aaatacaatt tacaaagata gctgaaattc atctaatcta tagatgtttt
     7621 cagctattta cttaagaaag aagtggaacg aatggataaa ttacaagtga tcgctgatta
     7681 tagtatggac caattagcgc atgatcaaac aggacatggg agcgatcata ccaaaagagt
     7741 tgtcaagtta gctgaaaaaa ttttggagac ggaaccgcag gcagaccgct ttgtgacgct
     7801 tgcagcagct tacctacatg atacgattga tgataaagtc gttaaaaatg agaacgaggc
     7861 aaagcaacag cttcgtgtat tccttcgctc tttgccaata gtagaggaac agatctcgat
     7921 gattttttcc attatcgaaa atatgtcctt ttctaaaaat ctctctgaag cggccgaact
     7981 atctctggaa ggtaagatcg ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg
     8041 cattttgcgt actgcttatt tcggcggtgg acatgggcac ccgatttttg atcctgaact
     8101 gtatccgcaa acattcaagg acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca
     8161 tttttatgaa aaactgttct tgctgtctga taaaatgaat actgattacg gatatgaaga
     8221 agccaaacga agagaaacct tcatgcgtga attcttagaa gaattttttc tggaatggtc
     8281 tattgacgat gaaaatttta ctccagacaa ttggatcaaa aataacaaaa aatgatggag
     8341 gttgtgacag aagtggtcag ctccaagata tttcggatca ttttccgtaa aagttacttc
     8401 tggaacactg tttaccaaga ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca
     8461 atttcaaaaa atagcttctc gtgtttgatg aatattgtaa cttgtgattc ggaattggca
     8521 ggagaacacc gtttattcgg aagaatagga ctgtgacaag acttttgtca cagtcctttt
     8581 ttcgaattca acaagtaaaa cttttaagaa atattcagag agtaaaatta aaaattgctg
     8641 ataaacgaag atggaaattt tatgtatttt tattggtcta tgcaataaat aaattaaata
     8701 ttcagaatac aattgatttt
//
LOCUS       DAJSMI010000111         8649 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJSMI010000111 DAJSMI010000000
VERSION     DAJSMI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.112"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            106..585
                     /locus_tag="ON430_002034"
     CDS             106..585
                     /locus_tag="ON430_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCR3219943.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            802..1893
                     /gene="phnW"
                     /locus_tag="ON430_002035"
     CDS             802..1893
                     /gene="phnW"
                     /locus_tag="ON430_002035"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCR3219944.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1886..2014
                     /locus_tag="ON430_002036"
     CDS             1886..2014
                     /locus_tag="ON430_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR3219945.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            2018..2563
                     /locus_tag="ON430_002037"
     CDS             2018..2563
                     /locus_tag="ON430_002037"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR3219946.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(3017..4708)
                     /locus_tag="ON430_002038"
     CDS             complement(3017..4708)
                     /locus_tag="ON430_002038"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR3219947.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(5128..6075)
                     /gene="arcC"
                     /locus_tag="ON430_002039"
     CDS             complement(5128..6075)
                     /gene="arcC"
                     /locus_tag="ON430_002039"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR3219948.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(6190..7209)
                     /gene="argF"
                     /locus_tag="ON430_002040"
     CDS             complement(6190..7209)
                     /gene="argF"
                     /locus_tag="ON430_002040"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR3219949.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(7300..8529)
                     /gene="arcA"
                     /locus_tag="ON430_002041"
     CDS             complement(7300..8529)
                     /gene="arcA"
                     /locus_tag="ON430_002041"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCR3219950.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2488 a         1750 c         1594 g         2817 t
ORIGIN      
        1 cctttttcca taaaataatt ttaggtataa aagacgaata ttattgggca atccttcttt
       61 tttttgttat gatacatgtg aaatctttcg taaaggagaa agaaaatgcg caaagctcaa
      121 agacatttgc tgattaaaca agtcattgaa gaattcccga tccgtacgca agaagaacta
      181 ttaaacaaac ttgggcatta tggcgttacc gccacgcagg caacgatttc tcgagatatc
      241 cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg tttcccgctt cgtattgttt
      301 cgcgaaaaag gaactcctga gactaggact gaggaagagg atcggctggt tcaaatgatt
      361 gaagacattg tattgaaagt cgaacgagtc cactttttga cgatcgtcaa taccttgcca
      421 gacaatgctc atttatttgc atctgtttta gatgagatca aacctccctc tattgtcagt
      481 acaattgctg gattcgatac gatcatggtc atctcggaaa atgaggagga cgcaaaacga
      541 gtagaagaat ttcttcacca tccaactgaa gtatcacttt tttaatcaga gcacgtattc
      601 ctaatatgga atgcgtgttt ttttattttc aatattttcc ttattgaata aacctttaca
      661 ctctttacaa aaatacatca ttttttattc aaatttttca acaccttgat tgaatcacaa
      721 taatcatcat ttatattgga tgttaacaag agcagcttct tgctctttgt gtatttcaaa
      781 aaagaaatga aaggaaatca tatgtataaa ttattaactt ctggaccatt gacgacttct
      841 gaaacagtga aaaatgctat gctgatcgat cattgtactt gggatcaaga atacaaagaa
      901 attacacaat ggataagaaa aaaattattg gatcttgctc aagtatcgga aggaatatac
      961 acaacggtct taatgcaagg tagcagctcc ttcggggtag aatcggtatt aagttctgtg
     1021 atcaggccag aagatactgt actgatttgt tcaaacggcg catatagaca acgaatgatc
     1081 aacatgtgcg aatgtctgaa actgaactat accgtttatg aggtatcgga acatcaaatc
     1141 ctccaagctc atgtgattac acagctgatt gaagagaatc cgtctatctc cgcactagca
     1201 atggtccata gcgagactac ttccggtatt ttgaataatt tggaagagat tgctgctgtc
     1261 acacaagaaa gagatctttt gtttatagtg gacgcgatgt caagctttgg tgggataccg
     1321 atttgtgtag ccgaactagg gattgatttt ttgatttcca gctcaaacaa atgtattcaa
     1381 ggtgttccag gcttttcttt tgtacttgct aataaagcgg aactggaaaa acgaaaaaat
     1441 gttagccgaa gtttgtcact tgatctatat gaccagtacc gcgtgatgga aaacgagaat
     1501 ggcaaatggc gtttcacttc ccctacccat acggttttgg cttttcaaca agcgctgaag
     1561 gaactagagg cagaaggagg aatcgaaact cgatattcac gttatctcgc caataatcat
     1621 gtgctacgac aaaaaatgac agagatgggg ttccagcctt tcattcaaga atatcagggg
     1681 ccattcatca cctcttttct ttaccctaag gactggcgat ttgattttaa tcatttttat
     1741 cagtatttaa agcaaaatgg atttgtgatc tatcccggga aaatcagcga tcatgaatgt
     1801 tttcgtatcg gaaatattgg cgagatctat ccggaagata ttttggaact gacagcaatt
     1861 atcaaagact ataaggagaa cgttaatgat taaaactgta atttttgatt gggcaggaac
     1921 aactgtggac tttggctgta tggctcctgt ccacgctttt agaaatgctt ttctagaaaa
     1981 aggaatccaa ctgactgaca aagaaatccg ctaaccgatg ggaaaattaa aatgggatca
     2041 tatccaacag cttctttccc tcccttctgt caaaaaacaa tggatagcta tttatggaca
     2101 actacctcag gaaaaagagg tggaggaatt gtatcaacaa tttgaacatt atctgtttaa
     2161 agatttggtc aagcattcta ccttgaaacc cgacacattg gaaacgatcg aacggttgag
     2221 aaacgctggg atcaatattg gttccacgaa tggatttaat gctcctatga tgacaatcgt
     2281 agcagaaacc gcaaaaaaag aaaggtattc accagacttc atggcaactt ttgaagacgt
     2341 cgaagggtat ggtcgaccgt ttccttatat gatccataaa aacatgcagt acttcaaaaa
     2401 caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc tcagatatcc aggaagggaa
     2461 aaatgccgga gtgttgacag taggtgtaat tgaaggtagt tcgctaatgg ggatttctta
     2521 tgatgagtac caaaaattag agatggggaa aaagatcgtt taaaacagtc tgttgctctt
     2581 agattccaac aagccggcgc tgatatttgt gtaaacaatt taaaagagtt aacagaatac
     2641 attttgtacg ctgacaagaa tccagaatga cttttataca aaataagagg ttgtgaaaaa
     2701 gctgtcctgt atcaagtaat aaaaacagcc aagcaaaaat agctcctcat atttttcgtt
     2761 tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattcga aaaaaaaaag
     2821 ggagctgtga cacgactttt gtcacagtcc ccttatttgc aaacaaacag tgtttaagag
     2881 acaacctctc cactaaacgg tgttcattca gctgactctc ggtatttgct caaaaaacga
     2941 taaaatttga aaagctattt tctcttttgt ttctctaata ctcggatcag aacgctgatt
     3001 tcacattctc tttttcttac atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt
     3061 ctttcaataa tacagtcact gcataaacta gagctaagcg tgcttctttt tgttcatcat
     3121 cagctaaaat cttcgtatga gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat
     3181 gtttcgcaat cactgacggt tcgtattttt ccccagcaga caagaccgtt tcagggtatt
     3241 tttgaactaa tttaaccact tcccagctgg tatcgtcatt caatgcatag tctgctgttt
     3301 ctgaagggac aaatcctgct ttttccaaca ggctgactgc acgagcatgt gtatattgca
     3361 cataaggtcc agtttctcct tcaaatcgaa caacttcttc caggttaaag tcaaatgtat
     3421 tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc aaccccaact tgtttagcta
     3481 cagcatcttt gttttccaaa tcagggtttt tctcgctgat ttgttcttta gcagattgga
     3541 ttgcttcatt taatacttct tctaataaga cgatctttcc tttacgagtt gataattttt
     3601 taccaccttg cgtgatcaaa ccaaatggaa tatggtgcat gtcgtcagac cagtcaaagc
     3661 ccatttcctt caagacagct ttcaactgtt taaaatgata gctttgttca ttccctacta
     3721 catatagaga ttgtttgaag tcgtaagtac gtttacggta caatgcagca gctaagtcac
     3781 gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa cgctggattc aggtcataag
     3841 cagataggtc aacgatctct gcacctttat cctcgttcag caaatgtttt tcttctaaaa
     3901 gtttgacgat ttcatccatc ttgtcattgt agaaagcttc accgttcaac gaatcgaaac
     3961 gtacttccag cagatcatag attttattga attctttcat cgattcatca cggaaccatt
     4021 gccataattg aatagcctct ttatcgcctt cttccagacg tttgaaccaa gcacgagctt
     4081 cttcatttaa ttctggttct gtttcagcta cttcatggaa ttggacgtat aaacgaagca
     4141 attcattgat tggttctgct ttaacagctt cttctgtacc ccattttttg taagcgacga
     4201 tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt gatgcggatt ggctgataac
     4261 cgattttttc cataatgaaa ccgatcgaat taccgataac tgtcgaacgc aaatgtccca
     4321 ttgagattgg tttggcaata ttaggagaag acatgtcgat tggtactgtt ccttgattcc
     4381 cgatgttgct atccccataa tgttcttttt ctttcactac ggtttgcaaa acttttttgc
     4441 tgataagttc tttattcatg aaaaagttca agtaaggtcc gacaacttcg attttttcaa
     4501 agttggcaga atcgattttt tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt
     4561 aaacttttgc taatgaaaag gctggaaatg caacatctcc gtgttcagct gattttggat
     4621 tttctagtaa ttgctcgact tgttccagtg ttaaatcatc tttgacagcg tcatagacag
     4681 cttttgcgac aagatcttta ttattcatct ttattcctcc taaagtgttc ccttgattgt
     4741 taaacaaaaa aaatctctag cgacggcata cttagtatgc catcactaga gacgagattt
     4801 cccgcggtac cactccaatt gtcctaaaaa aggaccagct ccatctgata tcggtagacg
     4861 accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt gtatcttctt cattcggctt
     4921 ccaccacttc cgaattcgct agccaagaat gatacaagta ctctcttgtt caacaaatct
     4981 tagtcttatt aaactttctt gaattatatc agaaaaaaaa acgaaaggct agtcgtttca
     5041 taaaaaaaaa atccagatgg cctaaaggag aagaaatacc acctggatag ctataaatca
     5101 tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc cttcttcaga
     5161 agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt ttggttttgc
     5221 ttcaacaaat gcaatagctg cttctacttt cggaagcatg cttcctggag cgaattgctt
     5281 ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct tttgatctgg
     5341 tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca aatccgcatc
     5401 gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt ctcgtccttc
     5461 taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga ctgtgataac
     5521 tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg gtttagggga
     5581 agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag tggagtcagc
     5641 attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt tacttggatt
     5701 tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa ccacatcttt
     5761 atcgatccct ttttccttca gtacttcgcc catggcgttt tgtaaccagt aaccgataga
     5821 tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct cagaatcagc
     5881 agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg agatgatcaa
     5941 ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta acgcttcttg
     6001 ttgggctttt gcacttgcat ccgtagacaa gatggcattg ccccctaaag cgacaactac
     6061 ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc tagataacaa
     6121 aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt gagatgaatg
     6181 tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca tgatcgcttt
     6241 gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc tgcggaagac
     6301 ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt caccgtaaac
     6361 agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat ctgtgttgtg
     6421 tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga tacgttcttc
     6481 gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt ctgctccgtc
     6541 tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt cagcaaagcc
     6601 ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa cgatacgcat
     6661 attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt ttcgaccatc
     6721 tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga tagttaaaaa
     6781 gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa ctggaacacc
     6841 agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt cgattccgtc
     6901 aaacatacgt cctagtactt tagctgtatc ttctgtggat tcttttttac ctaattggat
     6961 atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag tagtaaatgc
     7021 agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc cttctagata
     7081 gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat cgatcaaata
     7141 ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt ggaatactga
     7201 ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa gaggaaatcc
     7261 cgacggcagc tgacagtaag actgccgtca ggaaacaaat tacttcaagt cttcacggac
     7321 aagtggttgg ctcatgcagc gtggaccacc acgacctcgt gacaattcac ttgaattgat
     7381 ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat ttcggttata
     7441 agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc attgttctcg
     7501 aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat ctaatccaag
     7561 agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat catcaccatc
     7621 tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg gatggatagt
     7681 gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg cacggttgtt
     7741 cggaattttg attgccaaca ctttttcaaa tcctgaattt tttgcgaata ggtggcgtgc
     7801 taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta ctactttatc
     7861 gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat ccaaccatac
     7921 ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg taaacatcga
     7981 ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc ccattgatgc
     8041 ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg gatagtcatc
     8101 gtctgctgac agatcatata gactgctaga acgtacttcg atatcttttg tacgaacccc
     8161 agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact catgaagacc
     8221 ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca agaattgttc
     8281 tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa gagtctcgac
     8341 gccttcattt tgcaatgtct tcgcaaagtt gtcatgttct tcttgagcaa tcggaagata
     8401 tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt ccacttcttg
     8461 tccaggccgt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa cgtgaatagg
     8521 tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat cctccttttg
     8581 taatcacttt cataattttg tctcattcaa aatgagacat tcctcacatt aatattcgat
     8641 aattaaata
//
LOCUS       DAJSMI010000112         8488 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJSMI010000112 DAJSMI010000000
VERSION     DAJSMI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.113"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(361..3072)
                     /locus_tag="ON430_002042"
     CDS             complement(361..3072)
                     /locus_tag="ON430_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3219951.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(3082..3783)
                     /locus_tag="ON430_002043"
     CDS             complement(3082..3783)
                     /locus_tag="ON430_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3219952.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            3969..4568
                     /locus_tag="ON430_002044"
     CDS             3969..4568
                     /locus_tag="ON430_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3219953.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4649..5662
                     /locus_tag="ON430_002045"
     CDS             4649..5662
                     /locus_tag="ON430_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3219954.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            5770..6687
                     /gene="rbsK"
                     /locus_tag="ON430_002046"
     CDS             5770..6687
                     /gene="rbsK"
                     /locus_tag="ON430_002046"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR3219955.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            6715..7110
                     /gene="rbsD"
                     /locus_tag="ON430_002047"
     CDS             6715..7110
                     /gene="rbsD"
                     /locus_tag="ON430_002047"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR3219956.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            7110..8483
                     /locus_tag="ON430_002048"
     CDS             7110..8483
                     /locus_tag="ON430_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3219957.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
BASE COUNT         2530 a         1680 c         1622 g         2656 t
ORIGIN      
        1 tgtaaaattt atctttatac attctaagag gtctgttgcc caataaaaaa caagagatca
       61 gacatgtatt tgtctatcct cttgtttaaa atagcaaaat attcttgttt cttctataaa
      121 tggtgttcaa aagcttttat ctacggcaat cagtcttctc aaatgtaaaa catgaggagc
      181 taattttatt tgagtattct ttttgctcga tgcaggacgg cttttttaca acctctgatt
      241 catggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa tatgaaaaac
      301 tatttttccg tttgtttctc taatactcag gtcaaaccgc tgatttcaca acctctgttt
      361 ttattcattc gatttcaacg catcaatcat atcgatatgc tttaatttga aatgggtaac
      421 aatcatcaca ataaccgtaa atacgatagt taatcctgct gataagacat aagcccacca
      481 tgtgatcact aatggaaaga tgacattctc catagacgct tgttgcaaga tgaagtcagt
      541 caatatatat cctacaccaa atcctccgat gattcctaaa agagtaaaga tgatattttc
      601 tcgaacaatg tacatcgtga cttctttatc aaaaaatcca aggactttta ttgtagataa
      661 ttcgcgtaca cgctcggaaa tattgatatt agttagatta tacaagacga taaaagccag
      721 cagtcctgat aatacgacaa agattaagac aaccgagtct aagttcgcca ttgattcttc
      781 ttgcgtttcg atttgtttgg acatgaaaga cgtatttgtc acagcgtctg tggcaagcag
      841 tttctcggaa agcatttctt cttcttttgt tgtcatctta tctgtacgca gcaaaaatgc
      901 attatccatc atcgattgat cagtaactga agcataatat tcaggggaca tataaataaa
      961 gctacctaga tagtttttca cgatttcttt tacttcgact ttccattctt tttgatcttc
     1021 atcataaagc gaaatatgat ctcctgtaga taagtcaaat agttctgcta ctttttgcgt
     1081 aatagcgatt cctgttttag gcaaggagcg tgggttctct tcaagatcag ttaaatggat
     1141 aaaacgatcc aaatcagctg ttttacttgg tacgatcact gtcagacttt gcgaagcatg
     1201 tccttttttt ctcaattcta cggtttgagt atgaatagct aggatctcat ttactttttc
     1261 ttgcttctgc agcatatccg ctgctttttg acgactatct tccgtggatg aatccaatgt
     1321 aacgattgct tgatagtcaa tgatcgttcc aaattgtttg gctgaaacag aacttagaga
     1381 atctttcaat cccacgcctg caaccatcag cccggcacat ccagcaattc cgataatcgc
     1441 cataagcatt cttgacttat atcggaagag atttcggtaa ctgatttttt gattaaagct
     1501 aaggcgtgac cacagcggag tgatgtattc aagaaaaatg cgtttccctg gcttcggtgc
     1561 tttcggttga agcaatgcag caggccgttc tcttaaatct ttgaataaaa cgatcattgc
     1621 agcgccaaat gcggcaatga agaatgcggc agctgcttgt aaaatcggcg gccagtcata
     1681 aaaaataagc gcactcccaa tatcataccg ctcgttagac agctcaaaaa tgatccttgg
     1741 caaaagattc gttcctaata tcgttcctaa aacaatacct atccctgagg ctagacttgc
     1801 atagattgca tatttttttg cgatttcaac tttcgtataa cctaacgcct ttaatgtccc
     1861 gatttccctg cggttttctt ctaccatacg tgtcattgtc gtaaaggtga tcaatgcggc
     1921 aataaagaaa aagaaaacag gaaaaacatt ggcaatcgca gcgatacgat cagacagatc
     1981 accaaattct tgaaaaccga cattttctga acgttcattg tataggtatg atggtttagt
     2041 taaattttca attttttctg catttcgatt caattcttcc tctgcagagg ccagttcacg
     2101 ttctttctca actaattgcc tttcttgttc agcaagttga gcttcttgtt gagataatgt
     2161 ttgaacagcg attgttcgtt gcgcttcagg aagctgctgg atagttgctg actgttgcgc
     2221 taactgtttt ttagcatctt ctaaccgtgt tcttgcttgc gttgtttgtg ctttgccatc
     2281 cgctattttt tgctgtgccg gttctaattc ttcgttggcc gattttttca gctcttctaa
     2341 gcgttcttcg ggtcgatctt gtaaatactt ttctgccttt tcttgatttt gctccatttt
     2401 atctttgtaa gctttgctgt acgtattcaa attctttacg tttgtaaaat caagatataa
     2461 gacagaataa atatctgttt taaaaacgga ttcaggtaaa tagacaaaat aatcgacgct
     2521 accatttccg acgttcgcgt atcctctctc caaattgttg atgaacaatg gagaacggac
     2581 aaagcccgtg atggtatact ctttttcttt caattcatct gaatcaatct gataaatatc
     2641 accgatttta tacccttcat cttccgcttt ttcatccaat acaagctggt ttggtttttc
     2701 cgggagtttt ccatcagtta attgtagctt gttctgcttg ttatttttgt tataagaaaa
     2761 tatttgaatc acttcgtttt tgccagtatt ggaataatag atctgttttc ctgtttctac
     2821 ttttgcatct gggatctttt ctgcttgagc aatatctttt tgtgtcaagc caccagtaga
     2881 aacgatttgc aaatcagaaa gtcctgctgt accaacatag tgatccatcg ttttttgcat
     2941 gacaggtcca gcagttttga cacctacaaa taaaagtgtt cctagcatga caataaggat
     3001 gattgcaatg aatcgtcctt tagattgccg aatttcgcga aaacctgctt taagatatgt
     3061 tcttgttttc ataaaatccc cctaccatac aatctcatca atggatgaag gatgatcatt
     3121 taattcaaca gaccttactt tagcatcatt aatgtgaatg actctatcag cgatgggcgc
     3181 taaagcagaa ttatgtgtaa tgatcaaaac ggtattgccg tgttgtctgc tagcgttttg
     3241 caataatttc aatacttgct tgcccgtttc aaaatccagc gcgcctgtcg gttcatcaca
     3301 taaaagaagt ttgggatttt tagctagtgc acgagcaatt gaaaccctct gctgttcgcc
     3361 tcctgataat tgggacggaa agttattcaa tcgatgagct aggcccactt gcctcaatac
     3421 ctctacagga tcaagcgcat caggagaaac ttccgttgcc aattccacat tttcttttgc
     3481 tgttagatta ggaacgagat tgtagaattg gaaaacgaac cctacatctg ttcgtcggta
     3541 agcagtaagc tgtttatctg aaaattgcgc aatatctgtg tcatcaataa tgatctgacc
     3601 ttcatcaggt gtatccatcc cacctaaaat attaagaatc gttgactttc ctgctccact
     3661 tggaccaagt ataatcacta attcgccttt ttcaattgaa aaagaaatat cgtcatttgc
     3721 tgtaatggtc gtttctccca ttttgtatcg tttgtattca ttttttactt cgacatactt
     3781 cactctgatt cctccatttt cacgatattt ctcgctatat ctatgcatcg aatgcgtttt
     3841 ctcacactta tttattttac cactgaattt ctctagacta aagaaaggaa tcctttaatt
     3901 tagttgacaa ataaacgatt tatgatttac ttacataaaa aacattgtta tttgaaagaa
     3961 ggaacatcat gaatgattta aaggaaaata tgatttttga tttatcaaaa actgcttttg
     4021 tcgcgatcga catgcaaaaa gagatcgtaa ataacggtac actctctcca cacactgcag
     4081 catctattct gacaaacaat gatttgttag taagaacatt gaaacgtacc gctgccttga
     4141 aagttttagt gaacgtagac atctccactt tcccttattt gagtcaacaa actgatatgg
     4201 gtggaatgac tgcttctgta cctccagaat tcactgattt acttttgaag gattctttga
     4261 aagatacaga taatatgttg actatcacaa aatacaatcc aagtgctttt tttggtactt
     4321 ctcttgatct tcaacttcgc cggcgtggaa tcgaaactat cattttaagc ggtgtagcga
     4381 caacaaacgg tgtatatgct actgcattag atgcctttca acacgggtac catatcgtgc
     4441 ttgctgaaga tgcttgcagt gatcgtgaca aagaatccca tcaattgttc ataaaaaaga
     4501 tatttccgaa aactgcacga gtacgctcaa cgaaacaaat aatcgaagct atccaacaaa
     4561 gcaaataagt actggcacac gtgattttca tttacttctt tttgatttca tgggaaaata
     4621 accacaggat ttagatagga gtatgaacat ggtgaaaaaa aagatcacaa tcaaagaagt
     4681 cgctaaactt tccggcgttt caatcacaac tgtctccttg atattaaatg gaaatacgga
     4741 gaaattcagt ccgaaaactg ttcagaaagt tttagctgcc aaagaagaat taggctatca
     4801 gcctgactat tttgctagac aaatgattac caaggaaacg aaaacaattg gtgtactcgt
     4861 tcctgatatc acgaatccat tcttcagtac actgatgcga ggaattgagg atatcttgta
     4921 taagcagaat ttcgtcacga tattatgtaa tgctgattct gatcatcaaa aagaaatcga
     4981 atatctagcc gagctcacac gtcgaggtgt ggatggattc attatcgcga cttctgctgt
     5041 ctctacagat gcaatcaacg aaaatttgaa aaaacaaggc cggcccttca ttgttttgga
     5101 tcagaaaaaa tctgaaggat ttagtgatgc cgtacgaaca gacgattttc gcggtggtta
     5161 cctagcgggc atgcacttgc ttagtctagg acatcaaacg atcgcccttg tctatcctga
     5221 aaatccaccg gaaaatgtcc acgctcggat tgaggggttt aaatctgctc ttgatgttta
     5281 ccaaatccct cacgaccaac tgatcctctt acctacacag ttctcaaaac aaggtggcta
     5341 ccagatcaca gcagaacttc ttgattctgc tgcaactggt gtgtttgctt taaatgatga
     5401 attagctttt ggattatatc gcggattaga ggaagctgga aaaagcatcc ctgaagacta
     5461 cagcattatt ggctatgata atatagatat gtgcgagtat atcaagccga aactgacaac
     5521 gatcgcacaa ccaatcttcg aattgggaca aacatctgcc aagttattgt tagatcggat
     5581 ccaattccca gaaaaagaat gggaagaaaa aagattacct gtacgattag aaaaaagatt
     5641 ttcaactgca cccttgaaat agctttcaag gtgtgctata tttttatcga actattaaaa
     5701 cgttttacta aaacgtttta aaaaataatt ttcccatcaa aataaatgag atagttggag
     5761 gtttgtaaaa tgaacacggt tacagtaatt ggaagtatta atatggacac tacccttcgt
     5821 ttaaccaaca tgcccaagcc aggcgaaacg atgcatgcgc atgagatttt tcacgctggc
     5881 ggaggtaaag gtgccaacca agcagtggca gccaaacgtt caggcgcacg gacatctttt
     5941 attggaggcg ttggggctga cagcgaagga caacagttat tagatctact gacaaaagaa
     6001 aacatcgata catctgggat agctgaaatc caaggcgcca caaccggaca agcaatgatt
     6061 atggtagacg cagcaggaga aaatagtatc ttgatccatg caggggcaaa taatgctttc
     6121 catgaacaag aagtcctaaa aaacaagcaa ctgattacaa acagtgactt tattattgct
     6181 cagtttgaaa gtagcttaga tgcgacgatc ctagcttttt ctattgcaaa agacgcagga
     6241 aaaacaacga tcctgaatcc tgcaccagct cgtgaaacga ttcctactga actacttgaa
     6301 aagacagata tcattatacc aaatgaaaca gaaacagaaa tcattacagg aatcagagtg
     6361 acagatcata acagtttggt cgcagcagcc gaaaaactcc atgaattagg tattggaaca
     6421 gtaatcatta cactaggcag tgccggcgct ttctatcata cagaaaaaga acacggtatc
     6481 gtccctgctt ttaaagtgga tgccgttgac acaacagccg caggtgatac atttattggc
     6541 gcattaagca gcacacttca gccagacctt agtaacttga aagaagcgat cctttatgga
     6601 aatctagcct cttctgttgc tgtgcagtct tatggtgcgc aaccttctat cccttaccgc
     6661 gaagctttag aacaatcagc agtctaaaat taacaatgag aggaagaaac aaacatgaaa
     6721 aaaggaaaag taatcaattc agatatttca cgagtaatcg cacaaatggg gcactttgat
     6781 aaattaagta tcggggatgc aggcatgcct gtcccctttg gtactgaaaa aatagatttg
     6841 gctgtctcaa acggtcttcc aagtttttta tctgttttag aaaacgtttt agaagaactt
     6901 gaggtacaat cgattgaatt ggcggaagaa ataaagacaa tgaacccgga aatattggaa
     6961 aaaatcaaat tattgttacc agatacaccg attagcttta tcccacatga agaaatgaaa
     7021 aaagagttga ataactgtca tgcatttatt cgtacaggcg aaatgacacc ttacgccaat
     7081 attatattag tgagtggagt ggtcttctaa tggaaacgac aatgaatcct gaacagatgg
     7141 tactgtccga gcagattcat atgctgattg aacagttggg aggaaaagac aatattgatt
     7201 ctgtccttcc atgtgcgact cggcttcgta tcaaattgaa aaaacagcca gtagccaatg
     7261 cagaaaaatt aaaacagcaa atcccttcca ttaagtattt cattttactc gatagatatg
     7321 cagaaataat catggatcag ggattggctc ctgcttacaa ggaattgacg cgtcaaatcg
     7381 atcattcagc tccgaaagaa aagctaaatc cgatcgaaaa actatttggt ttgatatcgg
     7441 aaatctttac gccgatcatt ccgctgtttg ccggttctgg tatattaaaa ggtctggttg
     7501 ttttagcgac tacgattggc tggcttagtg aaacaagcgg aacttatcat attttgtctg
     7561 ctgcgtcgaa tgccgtcttt tattttctgc caatctttct tgctttttct gctgccaaaa
     7621 cgtttaaagt gaatggatat attgcggctg tcattgctgc agcactgatt gaaccgaatc
     7681 tgacctcttt actttcagac agtggcaaag cagctactga ttttctgggg attcctgtag
     7741 tactgatgca gtatacgtct actgtcatgc cggcgatttt aggtattttc ttttattctt
     7801 ttgttgaaaa attcctcaaa aaatatattc gcgaaaatat gcaattagta tttgtacctt
     7861 tactttcatt agttatcacg gttcctttga cgttaatggt attcgggcct gcgggtgttt
     7921 accttggcga aggtttagct gccgcaatta cttggatgat ggatattaac ggaccgatca
     7981 gtggcgcaat catcgctggt ggttggaata ttttggtaat atttggtatc caatgggctg
     8041 taaaccctgt catgatcagt aatatctctg cttatgggtt cgaccgaatc gttccgttga
     8101 caggagcagc caattttgga atggctggtg cagctttagg tgtatttctg cgaagtaaac
     8161 gttcaaaaac ccgttcgatc tccggttctg cttttgcttc gattttactt gcgggagtga
     8221 ccgaaccaac tgtttacgga attgcgatcc cattgaaaaa accatttgtt gctgcttgca
     8281 ttggtgcagc tgctggtgga gctgtaatgg gatttgccca agtgaaagcg atcgcttttg
     8341 tatttggtag tctgaccaca ttgcctgcat tcatttccgg tacttttttc tggtacttag
     8401 caggactggc tgttagtcta gtcgtagcaa tgatcactac tctcgttagc gggtttgatg
     8461 aagacctaat gtcttatgaa tagttaat
//
LOCUS       DAJSMI010000113         8398 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJSMI010000113 DAJSMI010000000
VERSION     DAJSMI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.114"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            78..2687
                     /gene="clpB"
                     /locus_tag="ON430_002049"
     CDS             78..2687
                     /gene="clpB"
                     /locus_tag="ON430_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR3219958.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2838..3524)
                     /locus_tag="ON430_002050"
     CDS             complement(2838..3524)
                     /locus_tag="ON430_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219959.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3502..3996)
                     /locus_tag="ON430_002051"
     CDS             complement(3502..3996)
                     /locus_tag="ON430_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR3219960.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4016..5236)
                     /locus_tag="ON430_002052"
     CDS             complement(4016..5236)
                     /locus_tag="ON430_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3219961.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5383..7218
                     /gene="lepA"
                     /locus_tag="ON430_002053"
     CDS             5383..7218
                     /gene="lepA"
                     /locus_tag="ON430_002053"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR3219962.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7312..>8398)
                     /locus_tag="ON430_002054"
     CDS             complement(7312..>8398)
                     /locus_tag="ON430_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3219963.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2695 a         1610 c         1692 g         2401 t
ORIGIN      
        1 gaccaaagcg atataatagt ttttgtaaag atcaaagaaa taagtgatct tacctttaat
       61 taggagggta tacctgtatg aatattgaga aaatgactac gacgcttcaa gaagcgattg
      121 ctgaagctca acagattgcg gtcactcgaa aacatcaaga tatcgacatt gcccatgtat
      181 ggaaaatctt tttacaatct aatcattttg gtcgaaattt ctatacggat gccggattgg
      241 atgtcgaatc ttttgaacat gaaattgatc gtctactgga tgagtatcca gttgtttctg
      301 gtggaaacgt ccaatatgga caaaacctca gccagaattt ctttcgatta ttgaatgaag
      361 cagaccaaat acgtgaaagc ttcggcgacg agtttctttc aacggaagtc gttattttag
      421 cgttgatgaa attaaaaaat tatccgttaa ctgtttattt gaataaaaac ggattatctg
      481 aaaaagaatt gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg acctcacaaa
      541 accaagaaga acaatacaaa gcattagaaa aatacggcgt tgatttagta cagcaagtca
      601 aaagcggaaa aatggatcca atcatcggtc gtgatgaaga aatccgtgat gtcatccgta
      661 ttctttcacg aaaaacaaaa aataatcctg ttctgatagg agaacccggt gtagggaaaa
      721 cggcaatcgt tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct gaaaatttaa
      781 aagataaaac gattttttca ttagatatgg gtgcattgat cgctggagca aaattccgtg
      841 gtgaatttga agaaagacta aaagcagtct tgaaagaagt caaaaaaagt gatggacgta
      901 tcctcttgtt catcgatgaa atccacaata tcgtaggggc aggaaaaaca gaaggaagta
      961 tggatgccgg aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat ttgatcggtg
     1021 ccacaacttt agatgagtac cgtcaatata tggaaaaaga caaagcgttg gaacgtcgct
     1081 ttcaaaaggt attagtcaaa gaaccaacag tggaagatac gatcagtatt ctgcgtggat
     1141 taaaagaacg ttttgagatc caccatggtg ttaacatcca tgacaacgca ttagtagcag
     1201 cagctacttt atctgaccgt tatattactg atcggttctt accggacaaa gcaatcgatt
     1261 tgatcgatga agccagtgca acgattcggg tagaaatgaa ctctatgcca acggaattag
     1321 atcaagttac tagacgtttg atgcagttgg aaatcgaaga agctgcactg aaaaaagaat
     1381 cagatgatgc aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct gaacttcgtg
     1441 aagaagcaaa tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc aattctgttt
     1501 cagcaaaacg tgctgaaatc gataaagcaa aacacgaatt agaagatgca gaaaataact
     1561 atgatttaga acgcgctgct gtattgcgtc atggtacgat acctcaatta gaaaaagaat
     1621 tgaaggaatt agaagctaaa gcaaaagaca gcgagattaa gatggttcaa gagtccgtta
     1681 ccgaaaatga gattgctcaa gtcgttggca gattaacggg tatccccgta acgaaacttg
     1741 tagaaggtga acgagaaaaa ctaattaaat tgaatgaaac acttcataaa cgagtgattg
     1801 gtcaagatga agcagttgat gcggtcagtg acgcggtaat tcgttcacgg gctggattgc
     1861 aagacccaaa tcgtcctcta ggttctttcc ttttcctagg accaactggt gtcggaaaaa
     1921 ccgaattagc gaaagcgtta gctgagaact tgtttgactc agaagatcat atggtacgga
     1981 tcgatatgag tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga gctcctccag
     2041 gctatgtagg ttacgaagaa ggtggtcaat taacggaagc tgtcagacga aatccttata
     2101 caatcgtctt gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat atcttgttac
     2161 aagtcctaga tgacggacgc ttgactgatt caaaagggcg agtagtagat ttcaaaaata
     2221 cggttctgat catgaccagc aatatcggtt cacagttact gctggaaggc gtgactgctg
     2281 atggaacgat tccagaagca gtggctgaac aagtcaacac gctattacgt ggaaacttca
     2341 aaccagaatt cttgaaccga atcgacgaca cgattttatt tacaccattg agcctagaca
     2401 acgtgaaagg aatcgttgac aaaatggtgg cgcaattggc acaacgtctg gaacatcaag
     2461 aaatcctgct gacaatcagt gatgaagcca aaacgtggat tgctgaaaat gcttatgaac
     2521 cagcatatgg tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa acaccactag
     2581 ctaaagaaat cgttgcagga catgttatgc ctaaatcaaa agtaacgatt actttacttg
     2641 atggacaact tcatttcaaa acagaagagc tagaagaaat cgtctaatct tccattgaga
     2701 ctataaaaga aaaaaggggt ctgacacaac tagtgtcaga cctccttttt tcaaataaac
     2761 agtgttccag aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc tgcccgcttc
     2821 tgtcacaacc tcttttatta ggtatttttc actttttcta atttataact ttcaaagtaa
     2881 cgctcatctt ttttactttg agaccatgca aacaatccca ttaaggcagc acctatcgta
     2941 tttgtcagca agtcgcccat cgtatccatc aatgctgctc gtccgacaaa cagtgtgcca
     3001 tcagaagcag caaaacgttg aagattcatg cctaagaact ggtcgcataa gaattcccag
     3061 aactcccaaa acacgccgca aagtccagca aacgcaaagc cgaataagag gaacaaccaa
     3121 ggactggttt ttgatatctc agcatctttc atcaagtaac caatcaatcc atagccaaga
     3181 gctgtcagga ccattggact caccgcatgt aagattttgt cccaaaaaga gatgatactg
     3241 atcaaatgca tccctgtgcc taaaaagaca gagatgaaca agaaaaacca gtaaaacaaa
     3301 acgattgctg ctggtatttt aagtttgaaa actttcaaaa tgacttgtgg catgaaaatc
     3361 aatgcaatcc ctaataaaga ttccacgata aatgtgattc cttgagaagt cgaatatttt
     3421 tgtgtgataa attcataaaa attatagcag acaacgaata caccaaaagc aagtaaagct
     3481 atttgcacct ttttgatttc tttaatcact gaaagtccct ccatctaaaa atctttttac
     3541 ttctgaaagc aactcaattt ctgaagaaac cacttttccg ttcaatttga tcaaacctat
     3601 tgtatataag ttcaggtagt gaaattgatt ttcagctgtt tcttgtagtg cagcgatttt
     3661 tttcgggtgg gttccgcctt gctgtcgagt atcagtatat aagccgatca ctgggatgcc
     3721 tttcgcataa gccacaccga tttccgatgc taccccggca tcgatcgtca gcccatctag
     3781 taaagcaatc attaaatcac tagccaacac tttttctgtg tcagcaagag caatcattcg
     3841 actgtctgca taagcagttt tgtcattgat tgcttcattt tcttgaggaa gatagatcga
     3901 aagctcagaa gagagctctc tgatttcttt aactaactgt tggttatata acaaatcact
     3961 tttagcaaac attggtgcgg caaaataaat tttcatctaa acatcatcct ttttatcaaa
     4021 aataatcgct aaagtaagct tccatgtttg gaatgatacg ctctagtgac ttcagtgttt
     4081 cttcatctgt ttctaaccgt tcgattcttg ccaaataaga agcacggcga taattcatca
     4141 tcaagcaagt caatgtttgg atatccagtc gaacggattt tcctataggt tcatttaaga
     4201 cacggacttg tccctgttca tcccatgtta aagcgaagac accattattc caaggagcga
     4261 caggatcttc aatgatgaaa tgaaaagcat cagctgttcc aacaaacgga aaccgttgaa
     4321 ggaattcttt tacatcaacg atccgagcca taaaatatgg ttcgatctgc tctttgatct
     4381 gactatcttc aatcaaaaat gctaaaggct catttttata aatatctcca tgtacccagt
     4441 agatcatgga aaaatgtgcg gaaataaagt tccataagcc atttcgagct tcttgatcaa
     4501 gatagaacat ttcttttacg tgaaatattt cttcagctac ccagtagaac agaacacctc
     4561 ttggttcatg gttgctatcg tagtagacag cggctgtgcg ctcatcttca ttttcgaaac
     4621 gccaatactc ttcccaatga aaatcactgc ggaataaagc accatggttt tgttgggcaa
     4681 atttgtcata aacggaaaat acgtctggat gatcaactgg taatcgctca accataccac
     4741 ttactgggat ttgtttaggt agctgtgtat ctctgatttt aaaggaaagt ttatcagaca
     4801 tgatttccca tcctttacgc cgatagtagg ggatactgta aggatacaaa taagagatcc
     4861 attgcttatt cttgcgcata ttatccaatg caacgataat tagatcttgc atcaatccat
     4921 ggttagcata ttctggataa gttccaactc cagtcacacc acccatttta taacgtgcac
     4981 cgtgaatatt gacttcacaa ggatagatag cgatttgaga gatcagttta ttttcgtgga
     5041 accatccgaa cactttcgat acttccaaaa tcggtttttt ggaacggata aaagctcgtt
     5101 tattctcaaa cccgctttcc tcgatgtctg cttcagtcac ttggaacaca taggaaagca
     5161 attcattgaa ttgatcgata tgttcttgag tgaccggttt taattcaaga ttttctctaa
     5221 aatctttttg atccattttt tcatctccat ttattagctt gataacagta tagcaaaatt
     5281 ctacaaaaaa aacttctata gctgactaat taaaaactct ttaaattact gcttttcgtt
     5341 ggtataatgg aaagtgctga ttatatgaaa agaggagacg ctatggatat aaagaaaatg
     5401 aaagaacgcc aagagcagat tcgtaatttt tcgatcatcg ctcatatcga ccatgggaaa
     5461 tctacattgg ccgatcgtat tttagaaatg acgcacactg tgacgtctcg cgaaatgcaa
     5521 gatcagctgt tagactcgat ggatttggaa cgtgaacgtg gaatcacgat taaattaaat
     5581 gctgtagaac tcaattacac agcgaaaaat ggagaaacat atatctttca cttgattgac
     5641 accccagggc acgtcgactt cacctatgaa gtctctcgaa gccttgctgc ctgtgagggt
     5701 gccgttttag tggtcgatgc agcacaagga atcgaagcac aaacattggc caatgtttat
     5761 ttagcgttag acaatgattt ggagatcctg ccagtcatca ataaaatcga cctacccgca
     5821 gcagatcccg aacgtgtccg tcaagaaatc gaagacgtaa tcggaatcga tgctagtgaa
     5881 gctgtattag ccagtgccaa atcagggatt gggattgaag atattctcga gcagatcgtg
     5941 gaatatgttc ctgcaccaag tggagactta gatgcaccgc taaaagcttt gatctttgac
     6001 tctatttatg atagttaccg cggtgttgtt ctaaatgtcc ggatcacgga cggtatggtc
     6061 aaacccggag acaaaatcaa actgatgagt aatggcaaaa catttgatgt tacagaggtc
     6121 ggggtcttct cgccaaaagc agttgctaga gactttttga tggtcggtga tgtcggttat
     6181 atcacagcaa gtatcaaaac tgtccaagat acacgagtag gggatacagt aacgttagca
     6241 gacaatcctg ccgctgaagc tttaccaggt taccgaaaaa tgaatccgat ggtttattgt
     6301 ggtctttatc caattgatac ttctcgttac aacgatttgc gggaagcttt agaaaaactg
     6361 caattgaatg atgctgcttt acaattcgaa ccagaaacct ctcaagcatt aggttttggg
     6421 ttccgctgtg gattcctcgg acttcttcat atggatgtcg tccaagaacg cttggaacgg
     6481 gaattcaatc tagaattgat cacgactgca ccatccgtta tttaccatgt taataaaacg
     6541 gatggtacga ctgctacagt agataacccg gcagattttc ctgaacctgt aacgattcag
     6601 gatgtagaag aaccattcgt caaagcacag atcatggtcc caaatgattt tgtcggtgca
     6661 gtaatggaac tttctcaacg aaaacgggga gaattcatta cgatggatta tttagatgat
     6721 taccgagtaa atgtcgtcta taatattccg ctttctgaaa ttgtctttga ctttttcgac
     6781 aaattgaaat caagcacgaa aggctatgcc tcattggatt atgaaatgtc tggttatcaa
     6841 aaaagcaagt tagtgaagat ggacatcttg ctgaatggag aaaaagtcga tgcattaagc
     6901 tttatcgttc accgagactt tgcctatgaa cgtggaaaag caatcgttga aaaactgaaa
     6961 aaactaatcc cacgccaaca atttgaggtg ccaatccaag cagctattgg acaaaaaatc
     7021 gttgcccgtt cagacatcaa agccttgcgt aaaaacgtct tggctaaatg ttatggtgga
     7081 gatgtttctc gtaaacgtaa actcttagag aaacaaaaag aagggaagaa gcggatgaaa
     7141 caaatcggct ccgtcgaagt tccgcaagaa gccttcatgg ccgttctgaa aatggacgag
     7201 gacgaaccga agaaatagca agggtttgtt tgaaaacagt gtaaaaaata aaccatgttt
     7261 acatggattt gccaccggtt tgccaccggt ggtttttttc ggtggcaaaa atcaagaatt
     7321 ctttctaaga ttttccatga tatcgaccgt ttcatttctc atcttatctg ttacatgcga
     7381 gtaagtatcc atcgtaattg aaattctgct gtggcccaat cgttcagaga tttctttcat
     7441 ttttgcgcca ttttcgagaa gaagtgtagc atgagtatgt ctgagagaat ggaagttaaa
     7501 agagagggag agtgcattcg atatcctcct agtattccat ttcactacac ttggcgtaac
     7561 tagctcaccg tcttccttcg tacatactgc atttgaatca atgtagagct ttccatactt
     7621 cattcgattt tctaattgtt gtttcttatg ttttttcaga attgctagca aggtttgtcc
     7681 aataaaaatc gttcgattgg aactgcttgt ctttggcgta ccatataccc atgcgccgtc
     7741 attctttacc atttgtttct ctacagtaat tgttccattt gaaaaatcga catcatccca
     7801 cgtcagacca caaacttcgc caacgcgcat tcccgtataa aatccaatat tcaaaggaat
     7861 atagaaagga tgtccttcag gagtaatttc tagcatatga tcaaagtcct caagagaaat
     7921 gatttttaga tcttttttag tcgttggtcg tgcttcatat tttggtattt ttacatacag
     7981 cataggattt tgcttgatta atccccaagg ataaaccgcc atattcagcg cattcttaag
     8041 gacagagtga gtaatagtca ttgttttctt cgagtaaccc tttttaaatt cagcattgat
     8101 gaaattttgt aaaagagcag gggagagatc cgtaagtttt ttctttccta aataaccgtt
     8161 tatatgattt ttgatggtaa atcggtagtt ttcataggta ttgtatttta gatttagttt
     8221 aacgtattcc tccatccaaa aatcaaggta ttgttttact cgagtatccg tacctaaaaa
     8281 gtattgtcct gtttcgtcaa tatctgataa aacttttcgt aaagcagctt cggcctctgg
     8341 tcgggtgtct ccgccaactt tctccacttt ttttcttgag ccatcatcat tgatatct
//
LOCUS       DAJSMI010000114         8273 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJSMI010000114 DAJSMI010000000
VERSION     DAJSMI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.115"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..640)
                     /gene="nth"
                     /locus_tag="ON430_002055"
     CDS             complement(<1..640)
                     /gene="nth"
                     /locus_tag="ON430_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR3219964.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMR"
     gene            complement(647..1345)
                     /locus_tag="ON430_002056"
     CDS             complement(647..1345)
                     /locus_tag="ON430_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR3219965.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1613..2797
                     /locus_tag="ON430_002057"
     CDS             1613..2797
                     /locus_tag="ON430_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3219966.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFVGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2803..3246)
                     /locus_tag="ON430_002058"
     CDS             complement(2803..3246)
                     /locus_tag="ON430_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3219967.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3472..3759)
                     /locus_tag="ON430_002059"
     CDS             complement(3472..3759)
                     /locus_tag="ON430_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219968.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(3930..5282)
                     /locus_tag="ON430_002060"
     CDS             complement(3930..5282)
                     /locus_tag="ON430_002060"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCR3219969.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5555..6001)
                     /locus_tag="ON430_002061"
     CDS             complement(5555..6001)
                     /locus_tag="ON430_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3219970.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6237..7163)
                     /gene="cysK"
                     /locus_tag="ON430_002062"
     CDS             complement(6237..7163)
                     /gene="cysK"
                     /locus_tag="ON430_002062"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR3219971.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7303..8190)
                     /locus_tag="ON430_002063"
     CDS             complement(7303..8190)
                     /locus_tag="ON430_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3219972.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2235 a         1708 c         1387 g         2943 t
ORIGIN      
        1 gacgcatccg atttttccca tcttgacata ttgatagcaa aggacatacc tcgcacttgg
       61 gatttcttgc cgtgcaatga tatctaccaa aaaaaatcag tgtatgatgt gtctttaccc
      121 ataattcttg tggtactttc cgcatcagtg tttcttctac ttccatgacc gtagcatcta
      181 gtttgcagat cctcaggcgt tttgagacac gttccacgtg ggtatctact gcgattgctg
      241 gtatcccgaa tgcatccccc aatacgacat ttgctgtctt tctgcctaca ccaggaagag
      301 acatcaattc ttctcttgac gttggcactt gcccatcgaa acgttcaatc agctgctgtg
      361 cacacgcttt gatgtttttt gctttatttc gatacaaacc aatcgttttt attttaagaa
      421 tgatttcatc gattgaagct tctgctaacg catcgggtgt cggaaaagag gcaaacaaat
      481 ctggcgttgc tttattcact gatacatctg tcgcctgggc acttaagatc actgcaatca
      541 ataactcaaa tggattattg tgcttgagtt ctccatgtgc ttcgggaaac attccataca
      601 tcgtttctag agcttccatt gttttttgtt tgcttaacat attccgctac tcctccaacc
      661 aatttttcag tgagactttc ggcaattctt cttgttgagc tgactgttct acttctcttt
      721 gaagaagctg ctgttttctt cgtttttggt cttcttccac ttgttgtttt gtacgtagat
      781 tttttctttc ccacgatagc aaaatgcggt caacgtaatt aaaactataa gcctgattta
      841 atacggcttc ccgcagtgct agcttcaaaa tttccggctg ataatggtct tcttctagcc
      901 attgtccgat ccgctggaac tcgatcgaag aaagcgggcg tccaaattct tgttcaaata
      961 attgatacac tgactggatt tctttttctt gtgttttttc ttcgtatttt ttttcttgtg
     1021 tttttaaata ctcacctaat agatcataga tggggtaaag attatatcga tctgcttttt
     1081 taccgttgac aatcgtcgtt tctatcttga taaatccatt tgttacgaga cgatccagta
     1141 tttgaaaaat ctgatcttgt ttgattccca tatccatagc aatcatctgc aaatcaggga
     1201 aagtatctcc ttcaagctgt gccatatgca actgtagaac aaataaaaat tcttcagatt
     1261 gtagaccgat cctatgataa tttttaagta aaagattaga tatgactgtt tgtccagcgc
     1321 ttaagtaatt ttctaaattc aacatgattt ctcctccgct caatcacagt atacgtaaaa
     1381 aaaacatggg aagcaagaac aggtttcttt tctatttact tcactgatcg gtcagattta
     1441 caatgtcgtc tacttgattt ttgtttgttc atgtttttga tgcgtatctt cacttttcgg
     1501 aataaataaa ttaagtagat ttcgctgttc cttagaatgt tgacaaggaa acaaaattac
     1561 tatacactat ttttgtttcc aagttaacat tattgcataa aggagttttt acatgtacag
     1621 tttcacaaat aaacattcta aagtttttgg atacgtctct gtcttattca ctggattagg
     1681 atttactatc gtcagtcctg tacttccgtt tttaacgctg ccttattctc actccataca
     1741 tcagcaagct ttctatatca ctttgctcat gtctgtttat gctctagctg ccttcttatc
     1801 tgcccccatt ctcggtagct taagtgatca ttttgggcgt catccgatcc ttattatcag
     1861 tttattagga tcaagtatcg gatatttgct ttttggttta ggaaattcga tatggatgtt
     1921 gttccttggt cgtatcattg aaggattgac ggcaggagaa atctccacct tatatgctta
     1981 ctttgccgac atcacggaac ctaatgaaag aaccaaagtt tttggatgga tgggtgcttt
     2041 agtcggtata ggaacaacgc tgggtcctat tatagggggt ctattagctg agttggggaa
     2101 ttctgttcct atatttgttg gagcactctt tactttctta aatgccgttt atggctacac
     2161 atttatgcta gaaagccttc caataaaaaa acgttcagta gatttaagtt tctcccatgt
     2221 aagacctttt caccaattac aacaattatt caagatttca tccgttattc ctttattgac
     2281 agcaggtttt gctgtttggt tggcagctgg ctctcttcaa tctatctttt ctcaattttc
     2341 gattgacgcc tttcagtgga aggctgggtt ggttggttta agcttctctt tgattggaat
     2401 cttggatact gtctcgcaat tgttcattat gcctcgattg ctcaaaaaat tttcagaaca
     2461 gcagatcaca agaataggga tgttcagtga aatcctcgcc tatctattca tcacattatc
     2521 tggaattctt ctactgccga ttttgttttt atttgggatt atctgttatg gtttcggtga
     2581 ttctattttt actcctgtat tcaatggcca actatccaat tctgtctcag ataaccagca
     2641 aggtcttgta atgggcggta cccaaagtat acaatctcta tcgcgcgtca tcggcccgct
     2701 aatcgcagga cagctttatg ctgcagcacc atttcttccg acggtattcg gatttttgct
     2761 tatgattttt gcctattatc actacaataa aaaatagtag cttcatttgc tacttcgtat
     2821 aaattctctg tatcgaccga taaaacgaaa tactgtgtca aattcttctt ttgacatatt
     2881 ttcaaataca gtagcatctc gctgtaccca tttttgatgg agttgctcgt gtctattcct
     2941 tattttttgt ccgattggcg tcaattgaaa atagatttct tttttattct cttcgctttg
     3001 ataagtgttt atgatccctc tagctatcag tttttttgtt agcttactga tggctcctct
     3061 agtcatgtaa ctagcattcg ctaattttgt cacgttagga tcagggattt tcccgatatg
     3121 ttcaatcact tctagttcat ttagagtaaa tcctgcaaat tctttttcca tttctggttt
     3181 attacgatga ataatctcat tagctaaatc cagatattct tctattagca gttgtctttt
     3241 atccatttct acaccttctt tttctttcat tatattgttt ttttgtttcc tattcaacaa
     3301 agataaaaat attttgtagg aaaacccaat ctgaaaccaa gaaaaaaagg ggttgacaaa
     3361 gcttgtcata accccttttt ctgattaaaa gatagtaaag agacagtcat cggtaattgt
     3421 gatgattttt tgtcatcact taaggatgag cggccttttc aaaaactgtc tttaaagatc
     3481 atcatcagtt tctagcacat atggctgacc ttcatatgtg tactctgtat tcaatgtgcc
     3541 gtcattttca tctgagtaga tgcgtctact gaaaggaaaa cgatgagaaa cattatcttt
     3601 ggcgtcattt gctggcatcg tgtaatattc ctcttgcgtt tcattcaact gagagagtac
     3661 ttgatctgcc aactcatcaa tattgatcgt taatttcttc gaagcacttt tataatcttc
     3721 taaaaatgca tcttgttgga caaaatattc tttattcatc ttctttctca cctttctttt
     3781 caaaatcaaa taaagctgct tgttctttta tgtaacagct ttatctttgc tgcttattgt
     3841 gacaaaaaat tcgaagaaag acaactaatt gtacttttca caaaaaaaga cagagggtcg
     3901 tgacaaaatt gtgtcacaac cctttttctt tattgtgcag tttcgatttc gcctagcgct
     3961 gcttgtgcaa gtaggttcaa gtaattccat gggcggtcaa aatgtggttg gaagaagaaa
     4021 tctgaaagtg cgagatcttc tacagtcatt ttattttgaa cagctaaaga catggtattt
     4081 gctgattgtg taatgtcgta tttggacatg aactgagcac ctacgatacg attcgttcct
     4141 ttttcataga ctagctccat cattactttt tcagtagttg gcatgaactc tggacgataa
     4201 ttatcttcaa atacagtcgc ttggacttct agatcgttca aagttgcgct ttctttggtc
     4261 acacctgtcg aaccgatcgt ccagccaaat aggtaaagtc ctgaagttcc ttgagtacca
     4321 cgatacgcca ttttatgatc tgtcaagtta tttcccacaa gcaaaccttg acgaacggca
     4381 tttgttgcca aaggaatata gttttgcgtg ccacttggat tgtagttgac tactgcagag
     4441 tctccagctg caaaaatgtc tggattactt gtctgcatgt attcattcac tttgattgcc
     4501 ccgtttggca gtgtatctac ttttccttcg atcagttttg tatttggacg gaagcccaca
     4561 cataaaatga ccatatctgt atcaaatgta tctgttggtg tagctacttg tttcactttt
     4621 ccttcttcat ctgtgataaa ttcagtaaca ttttcgccta gcgcaagagt aacaccgcgg
     4681 tctgtcaatt ctttttctaa gatatctgtg aatggtttat ctagatattt attcaagatg
     4741 cgatctaatc catcaatcaa tgtcacttgt tttccagatt cgacaaaagc ttctactaat
     4801 tcaataccga tatatccacc accaacaatt actacacgtt ttgcttcttt cgcttgtgca
     4861 ataatttcat ttgcttgatt gtagtttttg cataggagta catttttcgc atcgattcca
     4921 ctaatcggtg ggataatagg ccatgaacca gttgtcatca ctaatttatc ataagaaacc
     4981 gtttctgttt ctcctgtttt cagatctttt gccgttactg ttttattttc tgtatcgacg
     5041 tctgttacat cgtgttccat tttaactgtt gctcctaatg aagataattc ttcaggattt
     5101 gagtagaaca agcctgctgg gtcctttact actccgccga cataaagtgc gataccgcaa
     5161 gataagaaag aaatattgtc atttctttca tatactgtca cttctgcttc tggatgattt
     5221 tttaaaatac tttttacagc tgcagtacct gcatgagtac agccaattac tactactttc
     5281 atttatggtc cctccgatta acattttgtc cttcgacaaa actactataa cagaaataaa
     5341 acggatacaa cagtttaatg cttgtgaatt cacaaaggtt tgtttgtagt ttcattcctt
     5401 ttcaagcata agaacccaat atctacaaga ttcttatttt aataatattc acttttatat
     5461 ctaagattgt gaataaaaga caaaaaaata aaaaagctag cgtttttttt acacagctag
     5521 ctttttttag gtataacact tgtagaaact gtttttacat ttgcgccttt tttgctgact
     5581 ctttacaatc cggacagagt ccatacaatt ccaaccgatg ttcattaatc tcaaaatcag
     5641 tcagtctgct tgcagccatt tctatatctt caagccctgg gtaatgaaaa tcaacaatct
     5701 taccgcagtt ttcacaaatt acatgataat gacgctttga actgaaatca aatcggctag
     5761 aagcatcgcc gtacttcatt tcttgaacaa atccaatcga agtaaataag cgaaggttat
     5821 tgtaaacagt cgcaacactc atatttggaa accgatgttc aagtgcttta taaatctcat
     5881 cagccgttgg atgggaatga tgttcaatca aatattctaa aaccgcgtac cgttgaggtg
     5941 tgattcgaat gttctctttt tttaactttt cgataacctc ttcgactaac ttattttcca
     6001 tgtaaacacc ccacgttgtt attctcgtct tatcatacta tttttacatt ttaaaatcca
     6061 cttaacatga aaagaaaatt caattttatt gtattttaat gaaaaaagac gtaaaacagt
     6121 cgcgttcact attctacgtc tctattcaaa taatttttct catcataaat ttttgtgtgt
     6181 tcttttcatc tttaaaagtt ttttacataa acctatctgg agaaaaacca agtgccttat
     6241 atctcttggt aaagggctgt agataaataa cgttcaccat tgtcgggaac cacagctaat
     6301 accttcttgc cttttcctaa ttcttttgcg acttttagcg cggcagcaat tgcagcgcca
     6361 gatgagatgc ctactaaaat cccttctttt cgcccaactt ctctagctgt ttccatcgct
     6421 tcgtctccac taatagaaag gactttatca tacacagaag tatccagtgt tttaggaaca
     6481 aaacctgtac cgattccttg gattttgtga ggtcccgctt ctttcccttc taaaatagca
     6541 gattctgctg gttctacccc gattaattcg attttcggat acacacgttt taattctcgg
     6601 ccagcgccag tgatcgttcc tcctgtaccg atacctgcaa caaacgcgtc caagccattt
     6661 actccaaatg cttgatgtat ctcaggacca gtcgtcttct catgcacaag aggatttgct
     6721 tcattttcaa attgtaatgg taaaaagtag ccgttttctt ttgccaaacg ttctgcttct
     6781 ctgattgatc cagaaatacc atccgcacca ggagtcaaaa tcaactctgc gccgtaccct
     6841 ttcatcaata aacgtctttc gatactcatc gtctcaggca tcacgatgat tactttatat
     6901 ccttttgcaa ctcctaccat cgacaatccg attcctgtat ttccagaagt aggttcaatg
     6961 atagtatcac ctggtttcag tagaccatcg tgttctgctt tttctatcat acttaaagca
     7021 atacgatctt ttacacttcc tcctggatta aaaaactcaa gctttacaaa aacatctgct
     7081 gaatcttctg gtacgatttt atttaatttg acgattggcg ttttgccaat caattcggtg
     7141 actgagttat agatagctgt catcttcaat tcctccttgt ctttgctctt cttacttatc
     7201 ataatacaat ctgttcattt atggcgagtg attcgtcttt gactagcaaa aaaaagacaa
     7261 agatatacca tatatggtaa tttctttgcc tttttatcta aatcaaacgt ttagttcttg
     7321 accaactaat aaagaataac tggacataga catgccattt ttcgccatta gatcatcaac
     7381 agacatgcca aatttttgtg caatatccca tagcgtatca cctgacacga ctgtatatgt
     7441 ctgaccatta tttgtattta catctgttgt tgaagatgat gacgttgaag tcgtcgtagt
     7501 agtcgttgtt gttgtacttg tggttgttgt agccgaacct gttgatggcg tatcaaaacg
     7561 ggttaaatta taagtattaa tcaatgagtt caatttggcc ccataatttg gatcggtagc
     7621 ataacgacct gtcaagtacg ctgttgcatc ataaaaactt gttgtattgc ttttccacac
     7681 gccagcataa tgataatcgc ccgtggaaaa actggttgaa cgaagaacat tcgcatggtc
     7741 ttggaaggat tcccaataag aagaatattt tcggaatggc tcattcatcg ttacccattg
     7801 tccgtttaca tattctttgg ttgccatata aacgacctgt ccgttatagc ttcctttcac
     7861 accgaataga ttataatttg gcgcactagc taatccggaa gtgccatatg cactttccaa
     7921 caacgcttga gcgatcatga ctgaagcata caagtcatta cttgcagcaa cttctgacgc
     7981 agaccagccg atttgatcga taaacgcttg aggatcagcg actgtttgag atccttgttc
     8041 atctgcatga acatgttgcg gtaccgctgt aaccatcatc ggcccaacta aaagactcgt
     8101 tccaattaaa ggagcaatcg cttttttatt gatcaccatt tttcttttag gtctatggtg
     8161 ccgggcactg cgggtcttca gctcttgcat tcaaattcct cctcaaaata aaaaaactta
     8221 ataaaaagtt ctcttcttaa aacaaactta attaattaaa ttatactttt aat
//
LOCUS       DAJSMI010000115         8170 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJSMI010000115 DAJSMI010000000
VERSION     DAJSMI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.116"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            476..1339
                     /locus_tag="ON430_002064"
     CDS             476..1339
                     /locus_tag="ON430_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3219973.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ON430_002065"
     CDS             complement(1468..3090)
                     /locus_tag="ON430_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3219974.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ON430_002066"
     CDS             3339..3860
                     /locus_tag="ON430_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR3219975.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ON430_002067"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ON430_002067"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCR3219976.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ON430_002068"
     CDS             5941..6759
                     /locus_tag="ON430_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCR3219977.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ON430_002069"
     CDS             complement(6862..7059)
                     /locus_tag="ON430_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219978.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ON430_002070"
     CDS             7228..7806
                     /locus_tag="ON430_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR3219979.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ON430_002071"
     CDS             7901..8038
                     /locus_tag="ON430_002071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219980.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2446 a         1474 c         1612 g         2638 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagaaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta
//
LOCUS       DAJSMI010000116         7933 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJSMI010000116 DAJSMI010000000
VERSION     DAJSMI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.117"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            45..1673
                     /gene="treC"
                     /locus_tag="ON430_002072"
     CDS             45..1673
                     /gene="treC"
                     /locus_tag="ON430_002072"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR3219981.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(1733..2914)
                     /locus_tag="ON430_002073"
     CDS             complement(1733..2914)
                     /locus_tag="ON430_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3219982.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            3166..4689
                     /locus_tag="ON430_002074"
     CDS             3166..4689
                     /locus_tag="ON430_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR3219983.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(4758..5486)
                     /locus_tag="ON430_002075"
     CDS             complement(4758..5486)
                     /locus_tag="ON430_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3219984.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(5499..6482)
                     /locus_tag="ON430_002076"
                     /pseudo
     CDS             complement(5499..6482)
                     /locus_tag="ON430_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            6608..7900
                     /locus_tag="ON430_002077"
     CDS             6608..7900
                     /locus_tag="ON430_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3219985.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT         2728 a         1424 c         1533 g         2248 t
ORIGIN      
        1 aagttgtggt aggtaataaa tcagttgaat ggaggtaaat atccatgaca tttaaagaca
       61 aagtcattta ccaaatctat cccaaatctt acaaagacag taatggtgat ggaattggag
      121 atttaaaagg aatcaaagaa aaactcccgt atctgaatga tctaggcatc gacatgattt
      181 ggctaaaccc tatttatcca agccctcaaa aagacaacgg ttatgatatc tctgattaca
      241 cagccatcga tcctatcttt ggtacgatgg aggaattcga acagctaata gccgaagcaa
      301 agaaataccg gatagaaatc atgctcgata tggtgctgaa tcacgtttcg acagaacatg
      361 aatggttcaa aaaagcacta gcaggggacc agcattatca ggacttcttc attttaagag
      421 aagagccaac ggattgggta tcaaaatttg gtggaagtgc ttggtcgcca tttggagata
      481 cggggaaata ttatttgcac ttatatgatc ggacacaagc tgacttgaac tggcggaacc
      541 aagcagtaag agaggaacta ttcaaagttg ttcgtttttg gatggaaaaa ggggtaaaag
      601 gattccgttt cgatgttatc aatgttatcg gtaaagacga acaattgaaa aataataaag
      661 aaaatgatgg caaacctgaa tatacggaca aaccaatcac ccatacgtat ctaaaagaat
      721 tgaaccaagc aacgtttggg acggattcca ctatcatcac agtcggtgaa atgagctcta
      781 cgacgatcga aaattgtatc ttgtatacag aacctagtag agaagaatta tcaatggtct
      841 ttaatttcca tcatttgaaa gtcgattatg aaaatgggaa taaatggtct actcctccat
      901 ttgattttgc agaattaaag cgactatttc atacatgggg agaaaagatg agtgagggta
      961 acggctggaa tgctctattt tttaataatc atgatcagcc acgtgcgctc aatcgatttg
     1021 tagctatcga tcagtatcga aaacaaggag cagagatgct agcaaccatg atccatttga
     1081 accgtgggac gccctatatt tatatgggag aagaaatcgg catgatcgac ccagactatt
     1141 catcgatttc cgactatatt gacgtagaaa gtttgaatgc ttataagtta ctccttgaac
     1201 aaggattcac agaaaaagaa gcgtttcgtc ggatcaaagc aaaatctcgc gataactctc
     1261 gaacacccat gcaatggtca gacacagaac aagcaggatt tactacaggg aaaccttggt
     1321 taaaagttgg aggcaagcta gaagagatta atgtagaaaa agaactatct tctgaagatt
     1381 ctattttgtc ctattacaag aaattgattc ggctgagaaa aacatatcca atcgttgcac
     1441 aaggagatta tcatgcttat gaagcaaatc atccggaagt ttatggcttt cttcgccaat
     1501 ttgaaggaca gcagctgctt gtattaacaa atttctatga taaagataca aggatcgcca
     1561 tcccagaagg attcacagaa gccaatattc ttatcgataa ttattccgat actgttcttc
     1621 atcaaagtct ggagctaaaa ccataccagg caattgcttt gctgaaaaat taaaacaaaa
     1681 acacaaaacg tcgttcctac gagctattct cgtaagatcg acgttttgtg ttttatttaa
     1741 aataatcttc ttcagaaaat gaagagtttt catactctcc tttatcgcca taaaatttat
     1801 tgtagcgttg tttgaattta cggaaaataa aagtaacgag taatttgact aacgtataga
     1861 taggaatacc aaaaataacg cccacaattc ccattagatc acccatgaca agtaaaacga
     1921 ttaagatcgt aatcggatgg atctgtaaac gatcacccat tactcgagga atcaccaagt
     1981 caccgtggag aagttgtacg gcaaaccaga caatgacaaa tttgacagcc ataaaagtag
     2041 agtcttggaa tgcaatgatc aaacctggaa agaaggcgat aaacggccca atatatggaa
     2101 tgatacataa caacccagca gaaatagcta aaacactagc aaattctaat ccgatcagta
     2161 agaaacaaac ccagtagact gctccaagga taaatgaagc aatgatttgc ccctttagaa
     2221 atgaacccaa ttgttgatta gcaatctttg tcaaattttt aaaatctgta cgaaatgctg
     2281 gtgggacaat cgctaaaact gactgataaa atttttgagg atctctcaaa aggaaaaaag
     2341 cgacaattgg accagtaaat aaagtaataa aaatagctgt aacagttgaa aaaatgttgc
     2401 tgatccgttg tgcgccactt tcccaataat ttccaataaa tccagctata tcagacgtca
     2461 cgtcgtcaat agaagcgaaa aattgattaa atgaatcggc aaaaggtgtt ttctccaaaa
     2521 attgtcggag actctcttga aaatcttgaa acaaatctgg caaactactg atcaatgtct
     2581 ttgcctgttc ttcaagaaaa ggaataagcc atatgcttcc aagccccaat aaaaataaaa
     2641 taatcacata aacgataatg actgacaagt tcctcgaaag gtgcttgctc aaacgtttga
     2701 tgactggttc caaaagataa tacaagatca gtgcaaacaa taatgggggc aagatagtag
     2761 cgataatgat tccgacaggc ttgaatataa acgagatctg atggaaaaaa taaatgatca
     2821 gcgcaagtaa gataactatt cctaaaacat agtaagaggt ttttcctcct aaaaagcgga
     2881 tcaaacgatt cgttgtatct gtttcttttt tcactctttt tcctccttta tctataactt
     2941 cattatagac aaaagcgggt ataaaaggaa aatgatagag gttgattgca aaaatatcgt
     3001 caatcattca tataaacttt ctcatcaatt aaaataagaa aacgcttgac agttctttta
     3061 accatatgat aaattaaatc ctgttgctaa aacgtaacta ttacggtttg cgaaaagcga
     3121 atcggaacat tcaaatgaaa gaagatgaga agaggagtgt cataaatgaa acgattgata
     3181 ggtatattgg ccatgctggt tttagcaggt atgttaactg cttgcggggc aagtgggaaa
     3241 gccgaggaca gtaaagaaaa attgagcgtg atgacgacct tttatcccat gtatgatttt
     3301 acaaaagcaa tcgtcgggga tgaaggagaa gtagagttat tgattccagc agggactgat
     3361 tcccacgatt atgaaccttc tgctaaagac atggcaaaga tccaagatac agatatcttt
     3421 gtttataatg atgaaaacat ggaaacatgg gtccccgcaa tccaaaaaac attacaagaa
     3481 ggcaatgtcc atacgataaa agcgacagaa ggtatgcttt tacttccggg aagtgaagaa
     3541 ggacatgatc atgaccatga acacggggaa gaaggccata cacatgagct agacccacat
     3601 gtatggctag ctccaagttt agcaatcaag caggtagcaa acatacgtga tcagctgatt
     3661 gaagcttacc cagaaaaaca ggaagtatgg acaaaaaatg cagcagccta taccgagaaa
     3721 cttcaagcac tccatcagct ttatcaagaa acctttaagc aagcaaaaca aagatctttt
     3781 gtcacgcaac atgctgcatt caactatcta gcgttagaat atggattgaa tcaagtatcg
     3841 attgctggtc tttcttctag tgaagaaccg tctgcggcga gaattgctga actgaaacac
     3901 tttgttaaag aacatggaat caactatatt tattttgaag aaaacgccaa agatagtatt
     3961 gccagaacgt tagcaaacga agcaggtgta tctttagaag tattgaatcc tttggaagga
     4021 ttgacgaatg aacaaataga aaatggagaa aactatcttt ctatcatgga agcaaacctc
     4081 gaagcgttga aaaaaacgac agagacagaa aacccgctag aagatacttt gacccccaaa
     4141 aaagaaaaga gcgtctacaa cggttatttt gaagacgagg atgtacaaga tcgctctctt
     4201 tcagattggg caggaacttg gcaaacagta gatacgtatg tagaagatgg gacttttgat
     4261 cctgtttttg aatataaaga aaaattgaat caggataaga cagcacaaga atataaagaa
     4321 tattatacga agggctatca aacagatatt gcttcaatca agatcagtga gaaagagatg
     4381 gcatttacgt ttaagaatgg aacaacgaaa cgttcaaatt atcgttatgt agggaaagaa
     4441 atcttgacct acgaggcagg aaaccgtggc gtaaggtatc tattcgaagc agaagataag
     4501 gaaagtggcg cttttagata tattcaattc agtgatcaca cgattgcacc ggctgactcc
     4561 gaacattttc atttatatgc aggaaatgaa agtcaagaag tattgctgaa agaaatggag
     4621 aattggccaa cattttatcc agatacaatg gatggacgtg aaattgcaca ggaaatgctt
     4681 ttgcactgat aggcaaaaaa gaaccgaagg gtttggatcg gctggtcatt tagtcggtcc
     4741 aaactattcg gttcttttta tgaaatcaat ttctttaatc cacggataga ggctgggatg
     4801 gtctcgccgt ttgccaacgt gacttctctg gtttttttat taatcgaccg cacattttgt
     4861 gtattgataa cataggattt atgacaacgg ataaaattcc gatgcatttt ctcgatttct
     4921 ttgatcttct catagaattg taattgggca tgagtcaaat ggacttccag tttatgtggc
     4981 gctcctagcg aagtagaaaa atattggatg tctttgatcg gtaaaaattt gatctcatca
     5041 ttaaactcta ttttgaagat atctttttgg atttgggaca tttgaaaatc ttcgaaaatt
     5101 ttgctcagga tatgctcgac attttctttt aaatcataaa gatcttcttt gacgatataa
     5161 tcagtcggtt cgatgttgct ttttaagatt ttcatcgaca tttccgtatg ggaagtcaca
     5221 tagattattt ttcctaaagg atctaattca cggatcaatt taccgatcgc tacaccatcc
     5281 atttcactgt cttctagttc gatatctaaa aagtaaatac caaaagaaga agttctgatc
     5341 ttggctgcat tgagaagttc ttccggatca gaggtagaaa tatggacata actgtctaag
     5401 ttttcaatca tgattctatt tttgatcacc gttgataaaa cttctaattg ttttggatcg
     5461 tcttcacaaa tataaatggg aatcatatca ttactccttt attttatttg tatataaatc
     5521 gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg attggtcttt
     5581 gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt ttttgttgag
     5641 aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt gatcacacga
     5701 atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt ctttgcagca
     5761 ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag gtttaccgta
     5821 tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa ccctttttcc
     5881 tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt catattatcc
     5941 aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa tatcgtttga
     6001 tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg aagagaaaaa
     6061 agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt ttctagatct
     6121 ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc taatttctta
     6181 ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata tagaatgaaa
     6241 aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt ataaaaaata
     6301 atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg gcggtccaca
     6361 gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc agataaaaaa
     6421 gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac cgaagatttc
     6481 aggctctata ctttttgatg ttggtggatg acaccagcat cttttttatt ttccaaaaca
     6541 tatagaaaag acaccttgaa atcctttaga attaatttgg cgaaccaacc caaaaggagg
     6601 aatcaagatg tcttacactc accttatcaa agaaacatta gatattctag acttatcagt
     6661 tacttttaac gaaaattgtc tcacaaaaga aaaatacaaa gggcaaatct gtcatattta
     6721 tcgtggaaac ttgatttata cagcccaaga atgcatccac tgtaaacacc agattgcttc
     6781 agatattgtg cgttggggta caacaaccgt ccggttgtta atgaacgatg tttctgaata
     6841 ccgtacctac ctcgaattga aaaagcaacg cttcaaatgc aaagcttgtc agcggacctt
     6901 tgtcgcagat acttctgttg cgaaaaaaca ttgtttcatc agtgagaaag tccgctggtc
     6961 tgtcgtgact cgtctgaaaa aaaatacctc aatgacagag atcgccgctc agaaaaatct
     7021 atcggtctct tctgtttacc gtattatgaa gcgattttat cgtccgttga atccttttcg
     7081 gacaccgtta cctaaggtac tttgttttga tgaattcaaa tcagttcggg gtgtttcagg
     7141 tgctatgagt ttcatcatga tggatggaca aacacagcgc ttattggaca ttgtcgagaa
     7201 ccgacaactg ccttttttaa aacgttattt tagtcatttt tcacgagaaa ttcgtgaagc
     7261 ggtcgaatgg atcgtgattg atatgtacgc tccttatgta tcgctcgtta aaaagctgtt
     7321 tccaaaagca cagctgatta ttgaccggtt tcacatcgtc caacatattg gtcgtacctt
     7381 tcgcaatcat cgaatcaaag aaacgaacca actattaaaa agcaaagaac aaaaacacta
     7441 tcaattaggt aagcaattaa aaagatactg gaaacttctt caaaaagatg aacgcaagct
     7501 tgattatact cgacgccttt ggcgacctgg cttcaaagct catttaacag agaccgatat
     7561 tgtggatcgc ttactgaaag ggagccctgc tttgcgtgta ggctatcagt tatatcaaga
     7621 ctttctttac gctgtaaagg aacgagacta cgtatcgttt gaagaactac ttaccaataa
     7681 tatcatgctt cccgaaggct atcagacgac cttgagaacg ttccagaaat ttctgccaca
     7741 aatcaaaaat gctttacaac aaagttattc aaatggccca cttgaatgtc tgaacaacca
     7801 tatcaaagtg ttaaaacgaa atgcatatgg ctttcgtagc ttttataatt tcaaattgcg
     7861 catcatgatt cgtcatggca acgcattgat tttcaactaa aaaagatcaa gaggaactga
     7921 tccacttgat ctc
//
LOCUS       DAJSMI010000117         7912 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJSMI010000117 DAJSMI010000000
VERSION     DAJSMI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.118"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(200..718)
                     /locus_tag="ON430_002078"
     CDS             complement(200..718)
                     /locus_tag="ON430_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR3219986.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(720..1337)
                     /locus_tag="ON430_002079"
     CDS             complement(720..1337)
                     /locus_tag="ON430_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR3219987.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1334..2188)
                     /locus_tag="ON430_002080"
     CDS             complement(1334..2188)
                     /locus_tag="ON430_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3219988.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2185..3096)
                     /locus_tag="ON430_002081"
     CDS             complement(2185..3096)
                     /locus_tag="ON430_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3219989.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3137..4471)
                     /locus_tag="ON430_002082"
     CDS             complement(3137..4471)
                     /locus_tag="ON430_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3219990.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMVDENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4679..5509
                     /locus_tag="ON430_002083"
     CDS             4679..5509
                     /locus_tag="ON430_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCR3219991.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5753..6071)
                     /locus_tag="ON430_002084"
                     /pseudo
     CDS             complement(5753..6071)
                     /locus_tag="ON430_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6193..6387)
                     /locus_tag="ON430_002085"
     CDS             complement(6193..6387)
                     /locus_tag="ON430_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219992.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6892..7077)
                     /locus_tag="ON430_002086"
     CDS             complement(6892..7077)
                     /locus_tag="ON430_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3219993.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT         2615 a         1529 c         1329 g         2439 t
ORIGIN      
        1 aaatgatatc ctttttagtt aaaaaacacc aattatacaa catttattat taaaattaaa
       61 ttataaaatt ttataacgtt ctcatttaac aaaacttaag ttattaagac aaatgaataa
      121 acaactacgc tttcctcgaa acaaaaaaat aaagcacaaa tgcgcattta cgcactcatg
      181 ctttattcgt acaattaatt cagataacgg cagcgcatag gatgccaata acacttccta
      241 aaatagctcc tcctaaaatg tcagtaggat aatgtaaaag taaaaaaatc ctcgaataag
      301 cgatcaaaca agcaaataaa aaggctaata ttcctgcaac tggacttaat tcgaacaacg
      361 cagctactga taaaaaggct agcgctgtat gtcctgaagg aaaagaagaa gtcttcggtt
      421 tacttgtgta aaggacaagc cgacgatttt tttcataagg tcttttccgt cttgtcgttt
      481 ttttgataaa aagcaaacca acaaaaaaat agatcaaact accgataaga atggctagac
      541 catattctct agtatcaggt atcaagacta aaacagcagc aaggagcaac caaataaaac
      601 caccattaga gatattactg ataaatttca aaaaattgat ttttcgacta tccgttttcc
      661 gttgcttggc gaacttgttg ataatactat catcgatttt agcaatcaaa cgcaacatgt
      721 tattcctcct ttttaagaac cttttcttgc gtacgatgga taaaaagcag gacttgaaaa
      781 taaaacaaga aactgaaacc taataaagca aaatagattg gtcctgcaaa ccagaatgcg
      841 acaagaagga tcaacgtgcc tgccaaatag aaaagcgcgc ttttgaaatc attaaaaaac
      901 aagaaaaatg cagtaatcca agaagcttct ttttgttcca gttgaagcaa gattttgtaa
      961 ctaaagagaa gcgcgcttaa tagaagcaaa aacaacgtag tatacatcaa tgtatagcca
     1021 attcccccac cccaatagcg gaaaatctga taatcgatca atagaagaag aaaataaaca
     1081 cttgctaaac tatcttgctt tccataaaga gagaagttct cacgaaactt tttccagaat
     1141 agtgaaccga gctttttctc ttcatttttt ttgtaatgat aaatcatata cagaactgtt
     1201 cgacttgcag ttcctaatgt gaaaacaggc aagacgcaca ggaaccatag tagctgtaaa
     1261 tggataagac gtggaatcca ttcaatgatt tctaagaaat aatcattggc ttttttacca
     1321 atcgatcgat tcatcatcct tttgaacctc ctgtcaaatc catcccttca atgaaataac
     1381 gttgtgcaaa aaagaacagc aaaatcgtcg ggatcaagac aagtgttgct cctgccatca
     1441 gataattcca ttgtgtcgtt gcttggttct gaaaaacttg caagccaatc tgtaacgtgt
     1501 atttttcttg atcttggata tataacagag gacctaagaa atcattccaa gctgcgttga
     1561 aggaattgat cgcaatggta atcaaggcag gtttcgtcaa tggaagcatc aaatgactcc
     1621 aaatatacaa atgattcgct ccgtcgattt cagcagcttc gatcaattca ttattgatcg
     1681 ataagtaaaa ttgacgcatc agaaatatat tgaacgcatt gccgaagaac gatggaacga
     1741 tcaggggcaa ataagtacct acccagccga tcttggagaa taatacatat tgagggatca
     1801 tcgttacaaa ccccgggatc atcatcgtag aaagaaccaa agcaaacatc agctttttcc
     1861 caggaaaatc aagcttcgca aatccataag caataaaaga atttgaaagg acattaccga
     1921 tcactactag cacagtgatg aataatgtat ttcttgcata gcgccaaaaa ggaaaggcgg
     1981 caatcgtatc cagatagttt tcccatacga ttttttctgg gaaaaaggtc ggaggataac
     2041 gaacgatttc ttccattggt ttcaatgaag taaacaccat ccaaagcaat ggaatgatca
     2101 aaatgattcc tattactgtc atgaggacat aacttagtat tttttttgtt tgccgattca
     2161 cactggtctt tttgaaataa tccatcattt gccgtcacct ccagaataat agacccactt
     2221 aggtgcaagc ttcaaattga caaacgtcag aaccaacacg atcagaaaca ggatcattga
     2281 catagccatc gcataaccca tatcaaaagc tacgaacgcc ttgttccaca tatgcagatt
     2341 ataaaaaagc aaagaatttg caggtccgcc tgttccattt ttcgtcatga cataagcttc
     2401 ttgaaaaatt tggaaagaac cgatcgtaga ggtgatcaca tcgtaaaaaa taataggagt
     2461 gattaatggc aaagtaatac ttttgaactt tccccaaacg cctgctccat cgatttctgc
     2521 tgattcatat aaagatctag gaacattttg taatgtagct aagtaaagta gcattgctcc
     2581 tccagaactc catattttca tcaaaatcaa cgcaggtttc gtccattgtg gatcaaacag
     2641 ccaagctggt ccagggatat ggaaccagcc cagaaatgtg ttgatcaatc ctgcagatgg
     2701 agaaagcagc tgcatccata agatgtagac tgctactcct gataagactg ccggtaaata
     2761 gaaagctgta cggaatacac tgattccttt gattttttga ttgagtactg tggctaaaaa
     2821 cacactgaaa atcgtcgtca gcggtacatt gaagatgacg aaataaacgg tgttgtataa
     2881 cgatttccaa aataaatcat cgtgaacgaa cattcttttg aaatttgcta gaccaatgaa
     2941 attcatcttg gatgtcaaat tataatcggt gaaactacca ataactgaaa aaagcatagg
     3001 ccctagcata aataccaaga acccgataat aaaaggcgag ataaataaat aggcccatct
     3061 tgcttctttt ttctttcgga tagataaacg cttcaaattt tcttgcctcc tctgtaccta
     3121 gacagtcaaa aatggattat tgattttgct tgactaagtt ttctactgct ttttgtgcat
     3181 ctgccaatcc ttcttttggt gattttgttc ctaaaagaat ctggtcaatc tgttcattca
     3241 ctaatgtcgt gtaatccgga gcatctaaag gaactggtgt gatcaccgta tttttaaagt
     3301 tttcgtccac catttgatag atcatttttc ctttatccgt taggttgtca ttattcacta
     3361 aattctcatt ggctgtcttg ctcgccatga tatcaaaact tttttcgccg aatttttctt
     3421 gtacttctgg agaagaaaga tatttgatga attcataaga ggcctcagga tcttttgctc
     3481 cgtatggtac ttccaagacg aatccgccac cccacgacca atggcctgaa ccttcttctt
     3541 tttctgggag ctgcatcacg ccaaaatcaa aatcatccgg tgcattttcc atcaaactag
     3601 agtaataatt gatattttgg gcacgcatgg ctactaatcc ggaaataaat ggatcagata
     3661 caccagaccc aaattcggct tctaatttat tgatcgtatt tttcccataa taatcttgcc
     3721 agtcttggat ccactccaag gcttccactt tatttttagt atcaatcttg actttttcat
     3781 ctttgtcaaa ccaactagtc ccgttatctg cgtttagcgc ccatacatca gcccctaaat
     3841 tccataatgg ataaaaaccg atgcgtgtaa attcttttcc ttctttttta tctagtttac
     3901 gtgctgtttc ttctaattca tcccaagttg tcggtagatc ttgttcactg attccagctt
     3961 ctttaaataa ctttttatta taaaaaatga cttgtgtatc tgtattgaat gggatcgcat
     4021 aaggatcacc ttcatattct actgtatccc aaagctgcgg ataaaaatcc ttagaagtgt
     4081 ctttttctac atatttggag agattagttg cttgctgtgc ttctgctctt tgggcaacgg
     4141 aatttatgtc ctgtacaata acatcgggtg catttccagc tgttattgca gataaagatt
     4201 tagtccagat gtcaccccat ggctgatact tataatttac atggatctcg ttttgtgatt
     4261 gattgaaatc ctcaatgatt tcttcgatag cttcttttcg tgcaccggat ccccaaaagg
     4321 accagaaatc aatatttttt cttccatcac ttgttttttc agaagaattt gctgcacttg
     4381 aactggcatc gcttgcatta cttgatccgc atcctcctaa taaaaacaat gctgaacttg
     4441 ccagcaaccc tgtaaacaat aattttttca tattttacct ccaaatttta atgtaaccga
     4501 tgccggtttt aatataacga tatcattttg ttctgaaaat aacaatttat aaggtatgat
     4561 tctaaaaaaa ttattaatga taccaatact ttatataatt cgcgagcgct ttctttgaat
     4621 tttttaatct ttcgtagcag aatgagaatg tattaaaaat agattaggag gaattttgat
     4681 gtttagacac cagaaagaat tgcagtttga agcaaaacca gatcgtccga atccattaat
     4741 cgcgaaatat ctgcaggagc tgatcggcgg ccaatatggc gagatgagtg tagctatgca
     4801 gtacctgttc caaggctgga gctgccgagg agaagaaaaa tataaagata tgctgatgga
     4861 cattgcaaca gaagaaatcg gccacgtgga aatgttggcg acaatggttg ctcgtttatt
     4921 agagggtgcg cctgttgaag atcaagaagc agcgatgcaa aaagatccaa caatcggtgc
     4981 cattatctct ggaatgaatc cacagcacgc gatcgtagct ggcttaggac ctcgtcctgc
     5041 tgacagcgtg ggcaatccat ggagcggtgc ctatatcatt gcgagtggca acttgttagc
     5101 agactttaga gctaatttaa atgccgaatc acaaggaaga cttcaagtag cacgtattta
     5161 cgaaatgatc gatgatcgtg gcgtaaaaga tatgctgtct gttctccttg caagagatag
     5221 ctatcatcaa aatctatggg aatgtgcagt gaaagaatta gaagaaaaag aaggttcaat
     5281 cttagtccca agtactttcc cgcgtgacaa agaacgaaaa gacattgctt atgacttcta
     5341 taacttctca gagggagacc aaagtagtga aggttcttgg gctagtggca aagcactgga
     5401 tggcgaaggc gaatatcaat ggttgaaaga acctaagatc gaaggcgctg caccaaaatt
     5461 agaaccagta acaccaaaaa tgtatgggac acctccattc aagaaataaa tatctacccg
     5521 tagaaacacc cgttttttgt tccacacgct ttgtgagcaa aactgactaa agtcattaat
     5581 cataaccacc cttcaaaagg gtggttttct ctagagctat aagctcaaat caccggccag
     5641 cgcctaaaga cgctggcttt catattttat gttcaagcca tattgccttt gccagttttg
     5701 cccacctctt aaaaggttta tgtactactc cttagccctt aaaaggatcg tcttactctc
     5761 gtacactcag cttatctaac gcactatcat gtttttcttg ttcttgaatg tactttttga
     5821 tggtcgcttc attttgtcct acttttgtca catagtatcc ttccacccaa aagtgtcgat
     5881 acccaaattt atattttaga tttgcacaat ctcttgatga tctcctgaat attggctcga
     5941 tattgattat aaattatttt tcttctatac tttggtgtga atacaatgtg gtatttacac
     6001 atctactttg tacgggctaa actatttccc tttattgctc cacccgcata gcaagtggtt
     6061 ttgtatttca atcgctcggc gcgctcaatt aggtctaaag acaataaatc taaaaacccg
     6121 ctgcttcact gacagaaaca acgggttttc agaaaaaaat gaaatgaaaa agatctctta
     6181 cggcaatcta ttttattttt ttgatttttt ataaatggaa aatgcttcaa gaagtccaag
     6241 aaataaagcc gtataaaaac aagaacctat gaaatcttta tttattagat attcaacaga
     6301 aatcccgata acactagcca acaatatatt tatcagaagt gtaaaataag gatgcttttt
     6361 atagaataga actagtgttg ttttcataga ctctcctttc ttcatatttc caattaatca
     6421 caaattcacg gcattactag aaaaattaaa ggggcgcaga agtggaaaaa tactcttcga
     6481 ctttccctac aaaccaggcc atcttgtgtt taataactat accaaatcta aggtacctct
     6541 aaatctaaat tccatttgtg gcggtgacct ccaattttta actcttctat ctcccatcca
     6601 gtatacataa ccttgatact tcgaccactt atatatcgaa cacacaaata cctatccatt
     6661 gcaggagtac tacctaactt agttgctact tgcatctaaa taacagctga aaagatagga
     6721 cttactctta gtaaagtatc tgaactatta atttttaaac tatcattttt atcctcaact
     6781 acatgcacat ctgttgcaac ttcactttct gtctcatcca gataaatgat taaggaatcg
     6841 ggaattgcaa tatcttcagc tgacgctgga atagttgagc taaattagtc atcatatctt
     6901 tattataaaa taaccaacag caaaaaatac aatgaaaaac aaacttggaa ctaatgtatc
     6961 attaataaat gttgaaaaat tcagcaactc attactgtat gattttagca gcccaaccaa
     7021 catataccct ataaaagcta ataagatggt taggaatcct ttagaatttt tactcattca
     7081 tagacacttc cttaaataac ttttattata gaaggtattg taaataataa tattgacaaa
     7141 aagtgactca aaataaaaaa aaacttacat tatactggaa tgaactgtat tgatagtgta
     7201 cctaaaagct ctactgacat aagatcgtgt aatcggtaaa gtaattttat taattatacg
     7261 ggatgtaatc aattagagaa taaactaatc ataaaaggta cttttacatt cataattgtc
     7321 gctttgagat tctactttgg ttcactatct gacccaattg cacattttcg atcattacaa
     7381 gtattggtgt cgttagaaca actaaataaa aactagcggt agatttttca gaatctatgc
     7441 taatttctgt atggatgttc cagtgatggc aaatttctct tttcattgtt tttcttgtcg
     7501 catgatatgc ctttttgtgt tattcagtta aactatagaa attctttcct agacagatct
     7561 tgatcccaaa ttttacgttt ttgagcatcg atatagttta aaatgacatt actcatatct
     7621 aaagagttta gattttttag gactaaaact gtcagttgtt ctttttcttc tttctgttaa
     7681 agtattgtcg cgacttttcg tatacgttaa gatatgtcgt gaaaaaacaa gttttgtgac
     7741 acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt cgtttaaaat gctatatatt
     7801 ctttagttta agatacggaa attaatgcca tcgaccacac tagtagaatg aacgacattt
     7861 acttggcctt tttttaattt cgttatgttt agcatataaa aatattatag ag
//
LOCUS       DAJSMI010000118         7825 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJSMI010000118 DAJSMI010000000
VERSION     DAJSMI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.119"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..95)
                     /locus_tag="ON430_002087"
     CDS             complement(<1..95)
                     /locus_tag="ON430_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3219994.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGV"
     gene            complement(318..1349)
                     /locus_tag="ON430_002088"
     CDS             complement(318..1349)
                     /locus_tag="ON430_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3219995.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(1356..2186)
                     /locus_tag="ON430_002089"
     CDS             complement(1356..2186)
                     /locus_tag="ON430_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3219996.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAKTGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(2183..2998)
                     /locus_tag="ON430_002090"
     CDS             complement(2183..2998)
                     /locus_tag="ON430_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3219997.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDILSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(2995..3819)
                     /locus_tag="ON430_002091"
     CDS             complement(2995..3819)
                     /locus_tag="ON430_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HCR3219998.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(3809..4909)
                     /locus_tag="ON430_002092"
     CDS             complement(3809..4909)
                     /locus_tag="ON430_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3219999.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            5087..5872
                     /locus_tag="ON430_002093"
     CDS             5087..5872
                     /locus_tag="ON430_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HCR3220000.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            5905..6288
                     /locus_tag="ON430_002094"
     CDS             5905..6288
                     /locus_tag="ON430_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3220001.1"
                     /translation="MSNLRDYNQEAPIHCLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            6364..6495
                     /locus_tag="ON430_002095"
     CDS             6364..6495
                     /locus_tag="ON430_002095"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3220002.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            6464..6700
                     /locus_tag="ON430_002096"
     CDS             6464..6700
                     /locus_tag="ON430_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3220003.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(6778..7749)
                     /locus_tag="ON430_002097"
     CDS             complement(6778..7749)
                     /locus_tag="ON430_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR3220004.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         2252 a         1852 c         1544 g         2177 t
ORIGIN      
        1 tgaacgcctt tatattcttt ttcaatcagc cattcttcat caaaaaataa atgtttatct
       61 gttaatccga gtaattttct agtatgatta tccattgaga gactcctttg gtaggatgac
      121 gttttggtcg actaaatttt accaaaagtg agtctctttt tgagttgaaa cgaagaaaaa
      181 gtgttagtag aaaatcgcct tcgattttcc accaacacaa aatattatag aaccaaaatc
      241 aattctacct tagatgttct tatatcactc tatcagtcct atttgacaaa gagccagaaa
      301 tgtttgccga ctatacacta gctttgttgt gcaaaatgct gagtatcaat ttccacaggg
      361 attgctagaa tatctttcag ctgaatgtat aaggcatcgc taattttctc ttcccgatcg
      421 ttcaaccgat ccactaaaat ttctttgccc tccagcgttt gaaaaacata ccgttgaatg
      481 ttgcctaaaa taaatgattc cttccataga accggaatct taaccccctg aggaaccggt
      541 tcaaattgaa tcagctccgg tcttaaataa taaatgcagc gattgccctt agcggaaaac
      601 tcgtgggtat actgattcag ctcataccat tcgaaacgat tatagttgcc gataaattcc
      661 gcaacaaatc ggctctgagg attctgatac aattccttcg aggtggaaac ctgagccacc
      721 cgcccagctt ccatcacaaa aatccgatca ctgattcgca tcgcctcttc ttggtcatga
      781 gtcacaaaaa tcatcgtcat ccctagcgat tgctggatag cgcgcaaatc ccgctgcagc
      841 tgttttctaa ttcgggcatc caaagcactt aatggctcat ccagcagcag gactttcggt
      901 tcagtgacca atgagcgagc cagtgccacc cgttgtcgtt gaccgccaga aagaaactgg
      961 acatttttat ctgcctgctc cgtcatatcc accaatgata gcatctcaaa aaccttttcc
     1021 tgaatcacct caggactcag cttctttacc ttcaaaccaa aggctacatt ttcaaaaaca
     1081 gtcatagttg gaaacaatgc atactgttga aaaacaaagc cgatattgcg atccttcgta
     1141 ggcttatcgg caatattctc attgtccagc acaatctcac cgctctttgg ttgttccaat
     1201 cccgtgatac agcgcagcaa cgtagacttt ccgcacccgc tgggacctag taaagtaacc
     1261 agctcaccct tttcaatctg aatttcatca atagctaaaa tctgtttttc atcaaaaaac
     1321 agattgagat catgtatttc taaaaacatt ttccgtcaca actcctttaa gggcttcttt
     1381 ttcgactgct tctcttccct cgtctgctta agggacagct gttgcttttg tcgataagtc
     1441 agcataaagg tagttaaagc aattcccacc ataaacagca aatacaaaac catcacagcg
     1501 ctagccaaat ggccgttttc gttcatccgc cgcagcatat agatgcgtac cgtctgaaat
     1561 cgaccgccaa tcaacaggtt cgtcagcatg tactccccaa aaaccgaaga aaaaatcatc
     1621 agagaaacta gggtcacacc gatacggatg tttggaaaca gcacctggat aataatcgta
     1681 gacatcgggg cgcccaaggt ttctgctgca tccactaatt ccttcagatt aattaagcgc
     1741 atctggttgt taagactaag atagacaatc ggcaacgccg taataaacaa tgcacctacc
     1801 aacactacaa acattgggat accagtttta gcatacgttc gaagcaaagc cgttaccaaa
     1861 atcactcctg gtaaagcata cggcagcagg agcagctttt ccagccaacg attcaaacga
     1921 gggaaataca aatgaatcca aataatggtg ggaatcatca acaacagaat cgtcaccaag
     1981 accacacctg ctaataaaag tgagcgaccc accgcagcaa taaattccct gtccgtcagc
     2041 agctgttgga accattgcaa ggtaaaatca ctaggtaaaa gcgattttga ccatttagtg
     2101 gagctggcat acaaaatcgt tccaataatc ggcagcaagt aatacaggac aaagcagaac
     2161 aaaatcggtt ttattaactt tttcacgtct gcaccacctt gcctttcttt tgaataatca
     2221 acattgccgt taccagcagg acacctaaag cgactgccaa cgcactgcct aaattaggct
     2281 tggcgaacac atctcccgac actaaagccg caattcgaat cgttaaaaga tttacattac
     2341 tgctggtcag ggcatacgct gtttcatagg tccccatgcc attagcaaat aagatgacaa
     2401 aggtgctggc aatcgatggt cgcaaaaaag gcaaagccac cttgaaccaa gcatacgctg
     2461 atgaagcgcc cagcaattgt gcggactcca gccattcctt tttcacttcc ttcatgctgg
     2521 gatacaaaaa caagacccct aacggaattt ggaaataggt atacgtgatg gtcaatcccc
     2581 accaagaata caaattgaag tccgctagaa atggcaaaat cattttcagc acacctacat
     2641 tccccaacaa aataataaaa gagaatgcca gcggaatccc agcaaaatta gctgccaaat
     2701 tggaaacaaa ggtgattttc tcctgcaatt tagtcgatag attcattaag cataaacata
     2761 aaataaacgt cccgattaac ccaatcacac tggagctgac agcaatcagt acgctgttaa
     2821 caaaggcctg tgtgtaatat ttgttgctta aaatatcact gtaattggcc aatgtccact
     2881 ggttagttaa atcactgtgc aagctgcaca gaaccataaa aagcagcggc agcagtaaaa
     2941 atgcacagaa gataaaggag gcaggaacca gaaccaagcg ttttttcatc tctttcatcc
     3001 cctcatcaat tcttgaatgt cctgcatttt ctctgacggt gcaattccta ataaccagca
     3061 aaacaatgga gccgccagca gctgtggtac agtttgttcc ttcagctttt ggcgtttatt
     3121 tgaaatcaaa taaaaaggaa cttcacgatg tgcagccaag ctgccgccat gcagtccgtg
     3181 cgcatccatc ccgtgatccg cagtcaccac gacttgataa cctttttgca gccattcagg
     3241 aaggtaaata gccaataatc tgtccgcctg attaaccgct gctgcatatt catgagaatc
     3301 ggcggtaaat ttgtgaccga catcatcaat attcatcgaa tgtacgacca aaaagtctgg
     3361 ctgatactga ttcactaaat aatgggcatc cgcaaataaa tgcgaatcag ggtaagtgtc
     3421 ctcactataa aagatgccat gctgaatcag tcgctcctcc tgatgctgga tgcgatcaac
     3481 caaacgatta tagggggctt gattgtacaa ctcgctgtac cagtggtaag cggcggctcc
     3541 tgtttttccg ccagcttcct taactaattc aaacagcgac cactctttgg atcgctgagc
     3601 atggtaatta ctatagatgc cattttcata ggctggaacg ccggtcatca gtacttcgta
     3661 taacggacgt gaattgctgg gcagctcact ttttaccttc aataagcttg cttgctggtg
     3721 ctcaactaaa tgattcatga acccaagctg ggcgacggct gtgtcgtagc gacagccgtc
     3781 tagtataact acaatcagtt tgtcaagtct actttgcata ggtcagtacc tccgcctgcc
     3841 aaagctctgg aagctctaca gaggtcttgt tccaagcttc attgtcttca actggcaccg
     3901 ccgcttcata agcatcctcc ggcagtaatt tttcagctac ttctttaggc agctctacct
     3961 tgcgaatagg tcgcgcatag ccctttgcca aattgatttg cccttcatca gataaaatat
     4021 attcccgtgc tagctttgcc gcattgggat gaggggcatt tttattgatt aaggttgtat
     4081 acccgctaat gatggagccg tctgttggca cagttacctc aaaacgctcg gagtcaattt
     4141 ggtcacgata atttagtcca ttgaagtccc aaacaactgc cacatcgatt tcaccttttt
     4201 caagatttgc tacgctggca tcaacggtgg acaaacggcc tttttctgcc atttttttgt
     4261 agtaattcag acctggctga atatcctttt cgtcacctcc gttggcaatc gccgcagcca
     4321 atacagcaaa ttgtgcttgg ttggcaatcg tcacatcccc cacgctaact tgatagtcac
     4381 cctcttgcag ctccttccag ctagtcggtg cttgcttcac attctcttta tcggtgataa
     4441 aggaaatggt tcccgtatag cttaacaacc aatccccatc atcatccttt gcccaatccg
     4501 gaatttcctc ccaatagttg gtcttataag gtaaggttaa ctctttgctt tcagccaacg
     4561 gaccgaagct gataccgaca tcaccaatat cggcggttcc attttttcct tctgattcaa
     4621 acttagccaa ttcttctgca ctggacatat ccgtatccac gtgacttaag ccgtattctt
     4681 tttcaaggtc gttccaagtg cctacccaat ttgcccaagt atccggcatt cccaagctgg
     4741 caatctctcc ttcttccttg gctttttcta taatcgtatc taaagagtcg tctgccgcag
     4801 ccgattgctt cgttgcttca cctgagccgc agcctgtcag caggatactt actgttgctg
     4861 cacaaaataa accgatagtc atgttacgaa ttttttcatt cattttcatt cattcttctc
     4921 ccctttcgat aattccattc taacaaagct ttgtaatgag agaatcaatt tcttgaaaag
     4981 gtttcatgaa caatgtaaag gttgtatatg gtttgtaaat ttcgacgaat tcccgttaac
     5041 cttctaaagc ttttgcggta tgatagtaga aaaaaggagg atccctatgc agtattatga
     5101 tcagcatctc cacacgtatt tttcctttga ttcagaggaa aaatttgaaa actatttagc
     5161 ctatcagccg gagtttttcg tttctacgga tcactttgat ttaaacaatc catctaccgg
     5221 aaaagatgac attccggatt atccagccta tataaacaaa ctggactctt taaaagaaac
     5281 ctattccacc aaatttctgc gaggtatcga gattggcgtt gttccaggac aagaagagcg
     5341 gatcgtggat tacctcgctc agcatcctta tgatttaaag ctggtaagta ttcatcaaaa
     5401 tggccgcttt gattatatgg acgatgtggt tttaaccaaa gatcccattc aaactaccaa
     5461 ggaatatttt gatcagatgt tgcaggtctt gacggatttt ccagaaggag atattttgac
     5521 tcactttgat tatggattac gccgcttcac cttcacagcg gaagaattcg cggagcattt
     5581 tgaggctcag ctgctaacga ttttcaaaaa ggttgttgaa cgagaattgg cgatggagtt
     5641 aaatgccaag agcttcttga aatacggcaa cgaagacctt taccgttatg ccgttccttt
     5701 gtatcaaagt gtcggcggaa agctctttac cttaggctcg gatgctcatg ttgccgaaga
     5761 ttatcagctg ggctttaaaa aaatgagtca gctgttggca gactgtcagg tagaaaagtt
     5821 gctagtcatt caaggaaaag aacgccgcct gacgccgctg ccgattttgt gattcctata
     5881 taatagaaga aagtggtgaa aaaaatgagc aacctgcggg actacaatca agaggcaccc
     5941 atccattgtt taattgcgcg acattgggat gcgttgaaaa ttgaggcggt ttgccgatcc
     6001 ttgttagcag ccgttccaaa acaacaactg gaaaattttt tggtggctga cagcttgcag
     6061 cgggaaaaag ttcaggctta ttttgcagcc tttaaagatc agcctttgga atatttgcat
     6121 gcgcagtttc atctttttta tcaagtagcc gccccagatg actacaacga tttacgagga
     6181 cagctgcaat tgacctttca agcggatgaa accgcctata ccgttttatt gggaatggct
     6241 cgccttggtg atcaggcgaa ggtcgagtgg cggattttcg atatttgatg gcaggatcaa
     6301 ggcgtttcag ttgcaaacct tcctctcttt caattagaaa aaccgcagca ggaagaagtt
     6361 tgtgtgctgg tcaccgatgc tggtgaaatt cgtctccgct tatttcctga ccaagctccc
     6421 ctggctgtag ccaactggcg ctccctagct gagcaaggcg tttatgacaa cacacctttc
     6481 ggctcgtgtc attaaggaat ttgttattca aggtggcgct ttggatggtt ctggtgatga
     6541 agctgtttcc agctatgagg gctttttcgc tgatgaagtg catcgcggac tttatcattt
     6601 taatggcgcc ttggccttag gggaccacgg cccccacacc aacggcaacc agttttttat
     6661 tgtgcaaaac acaaaggcgc aggctgacct gctaatgtaa agcaagcata cttggagctt
     6721 ggaggactac cggagctgga tggtcgttat actgtttttg gataagtata ccaagggtta
     6781 gaaatcgtag cagcgatcgc agctcagccg acaaacagtg aagatgtacc gattgagcca
     6841 gtgttcattc gacaaattaa cttcgaaaaa acatcgtaaa aagacactca cagacttcag
     6901 caaaagctct attcttgagc tggctgcagc tagtgagtgt ctttcattta tccataagat
     6961 aaagcttgga ttcaataaat aaataaatgt tttggttaaa gctgtgacca gcaaatgagg
     7021 aaccgtgcga gccagttgat ctgccaatgc aaacatttct ttaggtgaag gcgctggcgt
     7081 gtctgccaac ggatactcca gtcccaatgc ttggtaaccg tgcataccat agcagtggta
     7141 cggtagtaat tctaacggta cgaaccgatc aagatctgcc acaaaacaag caatcgtcag
     7201 cagctcggtc aggttcatat tgatacctgg aaccacagga actcgaattc gcacttcgca
     7261 gttaggatac tcaattaaaa agcgacgaat attcttcaaa atttgttggt tatctaagcc
     7321 ggtccattgt ttgtggagct tgctggtaaa catttttaca tccacaaaca atgtatccac
     7381 atagggagct accttttgga tttcattata agcaccacaa aagctggttt ccagtacggt
     7441 attgattcca atgtatttcg atttttgcaa aatttcttta gcaaaatccg cctgaaccag
     7501 tgcctcgcct ccgctaatgg tcactccgcc atccgagtga aagtaaaaaa cagcatcctt
     7561 ctcaatctca tccattacct cttcagcggt catggtctgg ccaaatcctt tttcaatctg
     7621 cttgctttgt gattcgggaa tcgaacacca ggcacagcgt aatgggcagc cctttaaata
     7681 aacaacggtc cgtaaaccag caccatcatg tagagaaccc tgttctattc ttaatactgt
     7741 tccttccata tagatttttg aattacacac tagtttaaac taaaacacac aaggaaaaac
     7801 ttaccattta atcataaaat tagct
//
LOCUS       DAJSMI010000119         7807 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJSMI010000119 DAJSMI010000000
VERSION     DAJSMI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.120"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="ON430_002098"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="ON430_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCR3220005.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="ON430_002099"
     CDS             complement(2203..4389)
                     /locus_tag="ON430_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCR3220006.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="ON430_002100"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="ON430_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCR3220007.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="ON430_002101"
     CDS             complement(4611..5051)
                     /locus_tag="ON430_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCR3220008.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="ON430_002102"
     CDS             complement(5125..5664)
                     /locus_tag="ON430_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HCR3220009.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="ON430_002103"
     CDS             complement(6279..7745)
                     /locus_tag="ON430_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3220010.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2119 a         1722 c         1367 g         2599 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt
     7801 ttaacaa
//
LOCUS       DAJSMI010000120         7741 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJSMI010000120 DAJSMI010000000
VERSION     DAJSMI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.121"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..911
                     /locus_tag="ON430_002104"
     CDS             <1..911
                     /locus_tag="ON430_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HCR3220011.1"
                     /translation="KTVGKVPVDLGWKKLFGKESYRKEKEEEPLLPKVTKGEAVTVDL
                     QVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLK
                     QKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQKGTQATFL
                     ANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYH
                     KAAACTSEACDFRLWTTIAKKKLTATQLKEIIQNGRTSQPVKGLKGQKGSFEATIVLK
                     EDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            927..1076
                     /locus_tag="ON430_002105"
     CDS             927..1076
                     /locus_tag="ON430_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220012.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            1157..1753
                     /locus_tag="ON430_002106"
     CDS             1157..1753
                     /locus_tag="ON430_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR3220013.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            1766..2665
                     /locus_tag="ON430_002107"
     CDS             1766..2665
                     /locus_tag="ON430_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR3220014.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            2668..3153
                     /gene="ssb"
                     /locus_tag="ON430_002108"
     CDS             2668..3153
                     /gene="ssb"
                     /locus_tag="ON430_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3220015.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            3519..3779
                     /locus_tag="ON430_002109"
     CDS             3519..3779
                     /locus_tag="ON430_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220016.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            4226..4432
                     /locus_tag="ON430_002110"
     CDS             4226..4432
                     /locus_tag="ON430_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR3220017.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4432..4656
                     /locus_tag="ON430_002111"
     CDS             4432..4656
                     /locus_tag="ON430_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220018.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            4671..5108
                     /locus_tag="ON430_002112"
     CDS             4671..5108
                     /locus_tag="ON430_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220019.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            5101..5808
                     /locus_tag="ON430_002113"
     CDS             5101..5808
                     /locus_tag="ON430_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220020.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            6189..6368
                     /locus_tag="ON430_002114"
     CDS             6189..6368
                     /locus_tag="ON430_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220021.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            6461..6646
                     /locus_tag="ON430_002115"
     CDS             6461..6646
                     /locus_tag="ON430_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR3220022.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            6680..7111
                     /locus_tag="ON430_002116"
     CDS             6680..7111
                     /locus_tag="ON430_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR3220023.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         2787 a         1305 c         1581 g         2068 t
ORIGIN      
        1 taaagacagt cggaaaagta ccagtagatc taggttggaa aaagctgttt ggcaaagaga
       61 gctacagaaa agaaaaggaa gaagaaccgc tacttcctaa agtgacaaaa ggagaagcgg
      121 tgacggtaga tctacaagtc cttgaaaaag aaacgaagcc accacaacct tataccgaag
      181 gtacgttgat cacagcgatg aaaacagctg gaaaaaccgt ggatagcgaa gaagcacaat
      241 ccatattgaa agaagtagaa ggaatcggta cagaagcaac acgagcaaat attattgaaa
      301 ctttgaaaca aaaggaatat atcaaggtag aaaaaaataa gcttgtggtg acaaataaag
      361 ggattttact ttgccaagcc gtggaaaaag agccgctgct aacaagtgcc gaaatgaccg
      421 ccaaatggga aagttactta ttaaagattg gtgagcagaa aggaacacaa gctactttct
      481 tagctaatat ccaaaaattt gtctctcact tgttggaagt cgtaccagga caaatccaat
      541 ccactgattt tggatcgacg ttacaagagg tgaaagcagc cagtgaaaag caagaagccg
      601 cgcgtcattt agggatttgt ccaaaatgcc aggaacaaga agttttactc tatcataagg
      661 cggccgcatg tacgtctgaa gcttgtgatt ttagattatg gacaacgatt gccaaaaaga
      721 aactcacagc gacacagctg aaggaaatca ttcaaaatgg ccgaacaagc cagccagtga
      781 aaggattaaa aggtcaaaag ggttcgtttg aagcaacgat tgttctaaaa gaagacttca
      841 caacgagctt tgaattttct gaaaagaaaa aaacgaacta taagaaaaga acacgccgca
      901 ctacaaaata atggaggggt tatcttatgc gaaatacaac acacaaacaa caagaaatcg
      961 aaaatcaaaa agcaaaaatt cgttcagaac aagagaaatt acgtgccagt aaaagaagac
     1021 ttcagcaatt aaagaaccag caacggattc aaaaagaaat tgaattagat cgataaaatc
     1081 ttcctggcta ttgtatacgc ataaacttat gttatactat atttataagt ttatacgtat
     1141 acaaaggagg gagaaaatga caaaagcaat ggtttacaag ctgctagaag aatttaacgg
     1201 aactttggct ttgaaggatg ccaaaaaagc gggcatttct cctgtaacga tcaaacgtat
     1261 ggtagatcgt ggagagctgg atcgtgagta tccaggtttt ttcaccttac ctggtcaatt
     1321 tcctgatgaa ttgtttatgg cacaaaagaa atatgagcgc ggcattattt cgcatatcac
     1381 cgcgttggat ttatatgatt taacggatat gatccctaga cagatcgatc taaccgtacc
     1441 ttacggttat cacgtttctg aaaaggggtt gaaagaattc gcagttgagt tacactattc
     1501 aaaagcggaa tggtatgaat taggtaagat cgaaataaag agtcgttatg ggaatcctgt
     1561 tatagcttat gatccagaaa gaaccttgtg tgacatttgg aatccctggt acaacgttga
     1621 ggatgaaatc aaagtaaaag caatcaaaaa ctacatggag tcggacagaa aaaatcttag
     1681 aaaattgaat gagtatcgac gaattttgcc tacagacaaa acgatacgct cttatattat
     1741 ggctttaaat taaaggagaa gacacatgac acctacgcaa ttaaaagcaa aagtgaagaa
     1801 tatttccaga gaaaaagaag ttgatccgca gcttgtttta agacatttta tgatggaaaa
     1861 attcttggaa aaaatctcag agtctcctta tcgtgaaaat tttgtgttaa aaggcggctt
     1921 cttaattggc tcaaagtatg gcattgaaaa tcgaacaaca aaagacattg atacgacttt
     1981 acgggaaatg aaagtaacca aagaaacatt gacaacggtt ctaaacgata ttttttcaac
     2041 gccgacgaaa gaaggcattc aatttgagat tcaaggaatg aaagaaacaa gggaagctga
     2101 ttattatcca gggtttagct tacgtgtact ggcacatttg gaaaacatgc ggccagattt
     2161 taaggtagac gtgacgacag gtgactcgat ctatcctgca acgatcactc acagtcataa
     2221 attgatgttt gaaaatcgaa cgattccttt ggaatcttat cctacagaac aaattattgc
     2281 cgaaaaatta agtgcaacct ttgatttttt aacagataat tcgagaggaa aagattttta
     2341 tgatctctat actattccta aaatggaaaa gatcgatgag aagaccttga aagatagtgt
     2401 gagaaataca tttacacgtc gaggaaaaac tgatccgtta aagatttatt acgaaaagga
     2461 tatggatgta ctgaaaaata gtcatgagct aaaagatacc tggaagaaat atcaaaaaag
     2521 caatccttat gctgcgtcga tcacgtacga agagacaatg gattcgatta gtaatttgat
     2581 ggaaaaagtg attcacgtcg aagaaaaaga gcgaaaaata gcttatcaaa gacaccaaca
     2641 gatgaacaga gaaatggaga gataaaaatg atcaataatg taactttagt aggacggtta
     2701 accaaagatc cagatttacg ttataccgca agtggcacag cagtggctac ttttacccta
     2761 gccgttaatc gtaatttcac gaatcaaaac ggcaatcgag aagctgattt tatcaattgc
     2821 gtgatttggc gcaaaccagc agaaacgatg gcgacattag ctaaaaaagg catcttgatc
     2881 ggcgttgtcg gacggattca aacacggact tatgacaacc agcaaggaca acgtgtgtat
     2941 gtgacagaag ttgtagctga taacttccaa ctattagaaa gcaaagcagc gacagagagc
     3001 cgtgcgcacg ctgatcaatc gtccacgagt ccaagcacca ccacttttga acaacgggat
     3061 acagcgacgc ctaacaacaa tggattgaac gcttcacaga atccatttgg tggacaatca
     3121 atcgatatta gcgatgatga tctaccgttt taagtagcat tttctattgg ttgcatttgc
     3181 tacggtttat gttataattt agtcaacaaa aagaattttc aatctataac caaaaaccac
     3241 tagctggcgg gctagtggtt ttcttttacg tttctattat aagaattaac gagtaggctt
     3301 actgtctttc ttggcaaatt tacaatcaag ccatttgcag atatagtgcg cggctacttc
     3361 tgccatgatt gcaaaaacaa aatcaaaaag aattttcaaa cctataacct cctccccttg
     3421 ccagattagg ccggggtagc agacagcaag actattatag cataattgaa atatccaaat
     3481 gattggattt ttgctataat tttagcgagg tgattgtgat gaacaaatat gttggtttgc
     3541 ttgataaaat ccgagtaata aaaacgcaac cgttattggt tcgctttact ttacaaacga
     3601 ttcatgaatc gattaattgt gtagttgcag atattgaaat tatcgataaa ttgctaatta
     3661 tggatgatgg aaaatacaat attgccgtca caggtcactt caataaacga aatcagctgg
     3721 ttattgctag catgtatgtt agaaatcccg atcattttac acgatcgatg ggcatataaa
     3781 taaaagaaga gttgtagtcc taaatgagga ttgcagctct ttttgtttta catagaaacg
     3841 ggaacaggaa aaggcctgtt taggaaggca caagcgcctt ctcaattaaa aaacctttct
     3901 ctcactccaa ccgccaccgc cgttcgagaa gaaaggaagt gaacgctgca cgttcactaa
     3961 gatatggtgt cttgcaaagc aagacaaaat aggaaaagct tcctctcacc aaaaggttcg
     4021 ttcctgttcg ctatctcttt attgatttgc cagaccggca acaagttgcc ttttatcacc
     4081 aaaaagcgac aaggacaagg gtaaatggca cgctgcgcgc ccttgtcctt gtcgcttttc
     4141 gttgattcat tctggccaac aaatcaataa agaaagaact aacgaaagga agtaaaacct
     4201 ttcctaagag gaggcagaat caatcatgcg aaagaaattt gattattggg gtgttccctt
     4261 cagcgaatta ttcccaaact atcatgcacc acatactgtc gaatgcgatt gtggagaaag
     4321 agcgaaatgt atcaaaagct atcgtttgta tcaatgtcca acatgcggga aaaaatatac
     4381 cttgtcttat ggcgattaca ttttgattga tgagaaagga aagaaacgct gatgaaaaca
     4441 caacacaaat tattgaatgt aaaagaagtc atttctgaaa aagtagcaga agctttactt
     4501 attccgcggt actggacgat agaaacccct ttagtagagg acgcaccgac tatgctattt
     4561 gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaactt ggaaacttat
     4621 gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaat atggctcaga
     4681 attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa atggaggata
     4741 tgacgttcct ataccatgaa acacgtgtac ccaaaaaaca ttacaaggaa aaattgtcag
     4801 tcgcagtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg acgtactatc
     4861 gaacattgga agaactgaaa aaacaaaatg gaaaatggtt tttccaagcc ttactatgct
     4921 tggaagcagg cgtcaaacca agcacaatca aaccttctga ataccaagct ttagaactaa
     4981 cgtatggaaa atttatcgaa acgaaaaaag ccaagactgt ttcaagtgaa tggttggact
     5041 actttgaaaa tgtcaacaag tatggcgcat gttacatcat gaaaaaggag gacaacgaaa
     5101 atgagtaatg tggtttatca actaacagtg gaagatgttt ctcacttttt aacaaacaaa
     5161 gaacatttga atgaagtagt cagacggatt caaaaagact ttagttgtat ggaagaaatg
     5221 aagtacttta ttcaaagagt ggaagaacag ttgtatatgg aaagcgaaac gagtcaaacg
     5281 aaggtcacag tcagccataa tcatttagac gtgtataaac gtcgtcctga aacggtaaaa
     5341 aaaggaacag agcttctacg actagaaaca gaaaaataca tcatgaatgt gtacctagaa
     5401 aggagtgagc tggtcgaaaa ctttgtcagc tacacgattt gtaggaaaca ctattactca
     5461 gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga aatggaagag
     5521 ctaaacagca aaaatgcgac ggtagacgat tattaccttg tatttactag atttctgacg
     5581 tgtgggacgg aatctatcgt gttaatggga atggattatg aactcgctgt acccgaagca
     5641 tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta cacgacttta
     5701 actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa tgagcttgac
     5761 tgtaaacgaa tgccatatca acgatttgaa aggaaaaaag gtcaataacc atttataccg
     5821 aaaagtgaga atgtgtgaaa taagaacaaa aaaccttgaa attaagggaa tggttcttat
     5881 ttcgcaccaa tcgctccaac cgcccccgcc gctcaggtgg tgcgaaataa gcgggcaacg
     5941 accgataacc taagagaaag aaggaaatcc atatgaataa aatcattcct ctgttgttcg
     6001 tactgtttgt tggcgagata cttatgcgtc atgctgcagc tgtacattta aacttcgtag
     6061 tattcttagg aatactggta ttttgtttga tcttttggcg atttgtatta gcccttgtga
     6121 gtctactatt caagatgatt gcagccatat ttttcatagg gttatttcga tgaaaggaga
     6181 gagtactaat gccaattgca ataggaaata aacgtttacc cgtgacgttg gatgaaaaga
     6241 gacaaaaaga attgcaggaa ctcaaacaga agtacggcaa aagtgaatcc aggattatgt
     6301 gtattgcgtt agatttattg attgcacaag aaaaagcagg gtttgatgta ccagcactca
     6361 aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcactttac gcacgtgtcg
     6421 atacgtgtta tgattatttt gtaaggaggt atagtgcaca atgccactta cgggaaaaga
     6481 aatgcttaag ttattgaaaa aaaatggctg ggttgagcga aggcaagagg gatctcatca
     6541 tcatctttat aaggatggtg ttagaatcac tgttcctgtt catgccaatc aagatcttgg
     6601 ccgaggactt gagcaaaaaa ttttaaagga tgcgggattg aaataatctc gcattcgatt
     6661 aaagaaagga ttgactgaaa tgttaagtga gcctttagca aaaacctatc ctgctatttt
     6721 tagcccagaa gaaggtggtg ggtactttat tgaatttcct gatgttcaag gtgcttacac
     6781 aggaattaac gaagacgata tatcttatgg aattgctatg gcacaagagg tgttaggtat
     6841 ggttttagca gattatattg agcatgaaga tctacttcca gaaccaacac caatcaataa
     6901 gatttctgtt gaagatgatt cttttactac tttaattcgt gtagatgttg ccaaatattt
     6961 gaaagacaca gaacttgtca aaaaaacctt aacaattcct caatgggcgg ataagctagg
     7021 caaaagggct ggcataaatt tttcagtcct tctaacagaa tcaattgcag ataaagcaga
     7081 taacattctt cgttctggaa gaaataatta gaactcaaaa gagtcagaaa cttaaaaatc
     7141 tttggcttct ttttttacta caaagatggc ttctttaaat tatttgcctg attgatttaa
     7201 aaaaattgcg caaagactac gagctaatta agttgctcgt ggtctttctt tatgaaaact
     7261 ttttagatct agataaaaac attgagacac tttgaaaaat ttttttaata gtaatgggcg
     7321 cttctatgtc aagctgattc ttctttcggt aaattaattt tacttagaat atcactatct
     7381 tgataaaaaa tggtgtattc acaaattctg gcataaaaaa tacctacttt ctagttaagg
     7441 ttcctgaata agtcgcaatc caattatttc cagctgattt ataacttttt aaatataatt
     7501 ttccataata ccaataacca ttactttttt ctgtccaatt tcgttattga gaagatctag
     7561 aaggccagcc aactttatca aaaatcatca ttcctgtcac tcgtatcgtt ttagaagctg
     7621 ctcgagtaag taatggagaa actgttaaat aattttaagt aacagttaag tcgttagttg
     7681 ggttatcaat ggctattccg tccttatctg aacctcgaaa cccaatcata gaaaaagttt
     7741 t
//
LOCUS       DAJSMI010000121         7724 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJSMI010000121 DAJSMI010000000
VERSION     DAJSMI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.122"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            9..335
                     /locus_tag="ON430_002117"
     CDS             9..335
                     /locus_tag="ON430_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR3220024.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            403..1116
                     /locus_tag="ON430_002118"
     CDS             403..1116
                     /locus_tag="ON430_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3220025.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            1426..2277
                     /locus_tag="ON430_002119"
     CDS             1426..2277
                     /locus_tag="ON430_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3220026.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            2398..4251
                     /locus_tag="ON430_002120"
     CDS             2398..4251
                     /locus_tag="ON430_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3220027.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGAIIGFS
                     GVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDEQTQD
                     NQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPEVGEL
                     VAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLVKAGD
                     LLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            4299..5708
                     /locus_tag="ON430_002121"
     CDS             4299..5708
                     /locus_tag="ON430_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR3220028.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            5816..6517
                     /locus_tag="ON430_002122"
     CDS             5816..6517
                     /locus_tag="ON430_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3220029.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            6676..7155
                     /locus_tag="ON430_002123"
                     /pseudo
     CDS             6676..7155
                     /locus_tag="ON430_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         2563 a         1275 c         1726 g         2160 t
ORIGIN      
        1 gcaagcaaat gaatagtatg gaaaaagtca accagtttag agatgaacgt aattggcgac
       61 aatttcataa cgaaaaagat ttggctatct ctatttcttt ggaagcatct gaattgttag
      121 aactttttca atggaaaacg ccggaagaag taaaagaaac acaattagaa cgaatcaaag
      181 aagaactggc agatgtattg atctattcat acatgatggc agacaacctt cacctggatc
      241 tagatgagat cattgaagaa aaattgatca aaaataattt gaaatatcct gtagaaaaaa
      301 gcgcaggtgt acgaaaaaaa tacacagagt tgtagaaatg tgtcattcgg ctgttggaaa
      361 gagcaaaaga aaaacagtaa aatgagtctg gagggagaga aaatgaaaaa aaaaggaaaa
      421 gcaaaaagat ggctagtcaa tatcctcttg ttcctcttgt tattagtagg actagctttg
      481 attttcaatg aacagatcaa ggattatttt gtcagagaga caggagataa atatgctata
      541 gcgaatgtaa caaaagaaga tctgaaaaag aataacgata aagatgtcag ttttgacttt
      601 gatgcggtag aaccaatgac gacagaaggg gtcatgaggt cgcaaatgag aggaacagat
      661 ctaccagtga ttgctagtat cgcagtccct tcagtttcaa tcaatttacc ggttttcaaa
      721 ggcttggata atacgtcact actttacgga gcaggaacat tatctccaga tcaagaaatg
      781 ggaaaaggaa attatgcctt agcaagccat cgtgcaacga accctgaatt gctgtttaca
      841 ccgctagaaa atttagagat gggtgccaag atctatttga cagatttaga aaatgtgtat
      901 acatacaaaa cattttttaa agaaaaagtt gctcctacag atacccaatt actgaatgaa
      961 gtcgagggaa aagaaattgt cacactaatt acttgtggcg acatggacgc cgtcacgcga
     1021 ctagttgtac aaggagaact ggaaagtgtt acatcgataa aagatgcgac agatgatatg
     1081 agatctgctt tcaatctaga gacaaaaaca ttttaggata ctgggacata agtcgttaca
     1141 gattaaaaac agccaaagga tatgaatcga taaggaaaaa ccaatttgct gaaaaaaaga
     1201 gaaacgcaaa gaggtgcatc tctgatttgg ttgttttcta gatagacttg tgttccagtt
     1261 tttttatttt ttatatgaaa aggcttgcat aaataaaaat tcgatggtat gatgattttg
     1321 aataagaaat ggattgttac tgtacggcag gcaaaaccag aattcagcta agaaacagta
     1381 ggcgaattct aggttttttt atttttcttg agagggcgaa gcaaaatgaa aattgataaa
     1441 attttgaata acaatgtcgt catttcgaaa aatggattcg gggaagaagt cgtttgtatg
     1501 ggaaaaggac tagcttttca aaaaaagatt ggggacgaga tttcgccaga agcggtccaa
     1561 aaagaatttg ttttaagaga ttcttttgct aaaaaccaat tccagcagct gatggctgat
     1621 attccggcag aggagatcga atgggtcaaa caagtagtcg aactggctga agataaacta
     1681 aaagttgaat tttcgcctaa tatttatctg acattgaccg atcatcttca ttatgcgatc
     1741 acacgagcaa aagaaaatat ccaactgcct aatccattat tatttgaaac aaaaaaattt
     1801 tatccgaaag aatatcaagc agcgaaagag gctcttttgt tcgtgaaaca aaagacgggt
     1861 gtggaacttt ctgaggatga agccggtttt attgcctttc attttgtgaa tagccaacaa
     1921 ggaaacgaag acatgcaagt gacgatgacc gctaccacaa tggtaagaga tatactgagt
     1981 atcatcagta aattcttcgg catcgtgttc gatgaagaat ctttgaatta tcagcgtatc
     2041 attactcatc tgcagttttt tgcccagcgt tatctcaaag gggaaggatc tgacgagcaa
     2101 gatgaatttc tctacgcatt agttcaagga aagtatccaa aagcgtttca ttgtgtcgaa
     2161 cggatcaatg agtatcttcg acaaacgcaa aaacaggaaa tggggatcgc agagcagatt
     2221 tatttaacga ttcatattca aagagtagta agtgagaaaa aaagcatcca atcataagga
     2281 ttgttactgt atggcaggca aaacctggat tgaaaaaata ggttcacgaa tgtttgtgac
     2341 tgtttttttc aatctgggtt tttatttttt aaataaaaaa gaaagaagga aaaaacaatg
     2401 gataatcaag cagttggtcg tcgtgtatgg gaagcggtag gaggacaaaa gaatgtcaaa
     2461 agtttagtac attgtgctac gcggttgcga ttcagattaa aagatgaatc tttagcagat
     2521 acgcaaaagt taaaagaaga cccggacgtg atccaagtcg tacaaagtgg cggtcagtat
     2581 caagtagtga ttggcagtaa tgtagcagat gtttatcaat cgatcgtgga tgaggaaggt
     2641 cttgcagttt cggaggatgg aaaagaagag tccaaaaata ttttgaatcg tctgatcgat
     2701 attatctcta gtatatttac accttttcta ggggcgatgg cagccgccgg ggtattgaaa
     2761 ggattcttat cattggcaac ggtgatgggc tggctgacag ctgatagcgg tacttatcaa
     2821 atattgtttg ctgcagcaga tggtgtcttt accttcttac ctgtcatgct tgcttttaca
     2881 gcagctaaaa agttcaaagc caaccagttt ttagcagtag caattgctat ggcacttgtg
     2941 tatccagcta tcacagcagt tgctggtgcg ggagaagcta tcagtttctt tgggatccca
     3001 gtgattcttt caccatctgg ttatacttct tctgtcatac caatcattct agccgtatgg
     3061 gttcaaagta aattagagcc attcgtaaaa aaagtgattc cgcaatttct gcaaatgatt
     3121 ttagttccgc ttgtcgtttt agtagtgatg gttccattaa catttcttgc tttaggacca
     3181 atcgggacgg ttgccggtaa tgctttaggc ggtctcttca actcgattta tggatttagt
     3241 ccaatcgtag caggtctaat catgggaagt ttatggcaag tattcgtgat gttcggcatg
     3301 cattggggat tcgtaccgat catgtttttg aatatcgaac aatatgggtt tgatgtattg
     3361 atgccaatgc ttcttccagc catcttggca caaggtgggg ctgctttagc tgtagcattg
     3421 cgaacaaaag atacgaaact tcgagcatta gggatttctt ctacagtgac ttcgttattt
     3481 ggaatcactg aaccaactgt gtatggtgtg acattacctt tgaagaaacc attcattgcc
     3541 gcttgtattt ctggagctat tatcggattt tccggagtaa aagctttctc cagcagtttg
     3601 gtcagcttat tgacgattcc gactttcatt aatacggtgg atggtgtaga gtcaaatgtg
     3661 acagtagcag taatagctac aggaattgct tttgttcttg cattcgtagg tacattgatt
     3721 ttaggatttg acgaacaaac acaagacaat caattagaaa acaaacatgc gaatgcagga
     3781 gaaccgatta cttcagcacg gcatacctta aaaagtcctt taacgggtaa agtactgcca
     3841 ttgagtgaag taccagacca agttttttct tctggcgtga tgggaaaagg tatcgctatt
     3901 gatccagaag taggagaact agttgcacct gcagacggag agatcacgac tatttttcca
     3961 actggacatg ctgtagggat cacaacgact gacggagcgg agattttgat tcatatcggg
     4021 atggatacag tagaattaaa tgggaatggc tttgagatct tagtcaaaca aggggatctc
     4081 gtcaaagcag gagatttatt gattcgtttt gacatcgaag caattagagc agctggctat
     4141 agcgtaatca ctccagttgt cattacgaat actgatgcat ttgccgatat acttgaatta
     4201 gaccaaaaag agattattgc aaacgaagat gttctggcaa ttgtaaaata aggaggaaat
     4261 caggatgcaa tcacgttttt cagaaaaatt tttatggggt ggagcaactg ctgccaaatc
     4321 aattagaagg ggcttatgat cgagacggaa aaggactatc cgttgcagat gctatgcctg
     4381 gaggtaagca gcgctttgcg attatcggca gtgaagaatt tgactggacg attgatcaag
     4441 aaaaatatat ttacccgaat catcgaggaa tcgatcatta cgatcgtttc aaagaagatc
     4501 ttgccttgtt tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc tggacacgaa
     4561 tttttccaaa tggagacgaa ggtactccta acgaagcggg attagaattt tatgatcagc
     4621 tgattgatga gtgcttaaaa tatgatattg aacctgtgat cacgatttct cattacgaaa
     4681 tgcctcttca tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg atcgacttct
     4741 atgaacgatt tgctcagaca gtattagaga gatacagcag taaagtcaaa tattggatga
     4801 ctttcaatga aatcaattcg gcctttcact ttccagcttt aagtcaagga ttagtaaaaa
     4861 gcaatggagc aggagagtac caaaatattt tccaagcatg gcataatcaa ttcgttgcaa
     4921 gtagcaaagc cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc ggctgcatga
     4981 ttatctatgc aacaacttac agcatcgatg ccaatccagt caatcaggca gcaacaatga
     5041 tccaaaacca agaattcaat tttttctgca cggatgtcca agtacgtgga gaataccctg
     5101 catacacagc gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta gagcaaacgg
     5161 aaaaagactt ccagttgcta aaagaatatc cagtggacta catcggattt agttattata
     5221 tgtctacggc aatcaatgag actgatccac aagcagcaac ttcagaaggg aacttgctgg
     5281 gaggagtaaa aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc gatccagaag
     5341 ggctgagaat cgcattgaat gaattataca atcgttacca gaaaccatta ttcattgttg
     5401 aaaacggtct tggagccatt gatcatatgg aagaggatgg cacgattatt gatgattatc
     5461 ggatcgatta tttacgtcgt catatcgaag cgatggctaa tgcagtagca gacggtgtag
     5521 atttgatggg ctatacgcct tggggatgta tcgatcttgt cagtgcatca acaggagaaa
     5581 tgagtaaacg ttacggcttt atctatgtgg atctagacga tgaaggaaat gggacactga
     5641 atcgatcaaa gaaaaaatct tttgattggt ataaagaagt aattgcctca aatggcgtaa
     5701 acttgtaatt aaagcagctg aatgaaaaag aacacacttc tcacatgtga tttgtgttct
     5761 ttttttattt tttatgtgct attcttttag taatagtaaa gcaaagggtg acgttatgaa
     5821 aaggacacgt gtactatact tggaagtcgc agataaaatt aaagaagata ttttctcagg
     5881 aaaatatcca gtcggttcga tgttgccgac agaaagtgaa ttagaagaac tgtttcaagt
     5941 cagcaaaatc actgtccgaa aggcaatcga actgctggca tcagatgaat atgtcgcaaa
     6001 gaaaagtggt cgtgggacga cggttctaag caatcgtcct tacaatcgat tatccaaagc
     6061 ggcaagcttt acgcaaatct tggagaactc ccgatatgaa gtccggaaag aaacgctgga
     6121 tttgcaattg gtccatataa agaaggaaga tcctttgttt tcctttttcg gtgagcaagc
     6181 tgtttgtttt aagcgcttat attatttgaa caatcagcct tatatttatt ttgttcatta
     6241 tcttccacaa gaaatgatgc aattcagcag agaagaattt ggcaaagaat ctttgtatcg
     6301 tttgctgacg aaaaaaggat atactattga ctcattttct gatgatttct cggcagtatt
     6361 tttaacagaa gaagaacaag gaattctgaa aacagacgca cagatcggca tcaaacggat
     6421 acggaaatcg tctacgcaaa ttggggaaat gattgaatat tcggaggcgg tctaccatac
     6481 ggagctgcat ccttaccata ttgattatga aacataaata aaaaaagagg ttgtgaaaaa
     6541 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt
     6601 tggttaggct tattaccgca gatacacggc tgtttctgga ttaccattta ttctggataa
     6661 gggggtgtgg cacgattttc agaaggaatc tatgaaccaa tcactgtatt gattttagct
     6721 tcgatggtaa ccccattcgg cttgacgatc gcttatttct tagggaaaat cattcgtaaa
     6781 aatatactca atcgtcaaga aatcgataca ttgaagaccg cttttcctat ggggatctgt
     6841 cagatcacag agggatgttt ccctatcgta ttgaacgact tggcacgcaa tgtcatagca
     6901 acgggagtcg gtgggacagt aggtggcgga ttaagcatgt tttggggcgc agatagccgt
     6961 attccggcat cgggaatgtt tgcagtcgct acgatgacca gaccatgggc attcattggt
     7021 gcattgttag ctggttcttt tgtcacagga ataaccattt tattattgaa aaaaccagta
     7081 gatccgaatg cagaaatcat ccaagaagaa aaagaggaag aagacatatc ttgggatgat
     7141 ttgacaatta gttaggagag gcagaatgga ttacaagaaa aaagtacaat agatgtacct
     7201 tgagacagga atggtatgaa agaatcagag ttagaaagaa tcgaatatac agattttggt
     7261 ttaggagcga tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac gatgaaggaa
     7321 aaatgagaac ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag gaaaggaaac
     7381 aaaaaaactt tcctgtacta ttccaaaaag gaaagaaagt tattatacag taaaaaaaga
     7441 aattattctt catgctagag atcagtacac gttcgagccc aatatcaaac actagttcca
     7501 ggcaggcgca acaggtgcgg ttatcactga attttcttcc agcagtgacg attcaagtga
     7561 tatttttaca aatccagcta tttatcgtta acaaaaagat ttttaggaga agctgatgaa
     7621 caaaaatgat caaattttga tcgtttagtt cgtcggcttt tttatttaga gaaacatcat
     7681 ttatcagtga aacataacac gagaacaaaa tgattttttc gtta
//
LOCUS       DAJSMI010000122         7700 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJSMI010000122 DAJSMI010000000
VERSION     DAJSMI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.123"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            544..1107
                     /locus_tag="ON430_002124"
     CDS             544..1107
                     /locus_tag="ON430_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3220030.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="ON430_002125"
     CDS             complement(1386..1673)
                     /locus_tag="ON430_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3220031.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="ON430_002126"
     CDS             complement(1663..1992)
                     /locus_tag="ON430_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3220032.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="ON430_002127"
     CDS             2297..2626
                     /locus_tag="ON430_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3220033.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="ON430_002128"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="ON430_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HCR3220034.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /gene="vanY"
                     /locus_tag="ON430_002129"
     CDS             complement(3551..4462)
                     /gene="vanY"
                     /locus_tag="ON430_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HCR3220035.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4890..5498)
                     /locus_tag="ON430_002130"
     CDS             complement(4890..5498)
                     /locus_tag="ON430_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR3220036.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="ON430_002131"
     CDS             complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="ON430_002131"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR3220037.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6528..7496)
                     /locus_tag="ON430_002132"
     CDS             complement(6528..7496)
                     /locus_tag="ON430_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR3220038.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2353 a         1532 c         1280 g         2535 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tagttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat
     4801 atataaattc cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta
     4861 tatagtttgt ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg
     4921 gggtatggtt cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca
     4981 ctgttttcca tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca
     5041 tttgccgcat gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc
     5101 tcacccgtgt ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat
     5161 tttgaagcca cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt
     5221 tcctttgtca ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacga
     5281 ttaggacggt aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt
     5341 gccttcaaaa gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg
     5401 tcaaccggtt ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg
     5461 tgtactattt catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta
     5521 atacgatcaa gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg
     5581 gataacgact gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc
     5641 cgttatcttg taaaaacata tccacacggg ctagacctct acagccgagc gctttatata
     5701 ttttttttgc cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg
     5761 ttataactgc gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc
     5821 cgtactgcag cctgatttgg tccacctcgc caacagctaa cgcggcactg tttcccaata
     5881 ccgcacaacc gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt
     5941 gtcttgccga ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga
     6001 aggatgagcc tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg
     6061 gcctatcatc tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa
     6121 cgatgtatgt caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa
     6181 aagggatacc ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat
     6241 gcaaagctga aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa
     6301 gtaatccgtg cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc
     6361 attccgcgca aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata
     6421 acggctcgta tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata
     6481 cgtcatgctc ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc
     6541 tgtctccttt caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct
     6601 tgctcggtat aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta
     6661 agtaaaaatt gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct
     6721 ccttccaata catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac
     6781 tcataggtat ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt
     6841 ctctgtattt gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc
     6901 gtaacgatat cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt
     6961 cggctgcgac tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct
     7021 ttgcctatct ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg
     7081 tcgctgtcca acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt
     7141 actgccataa gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca
     7201 ttgtcgacag tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg
     7261 cttcgggtag aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa
     7321 atctctgatt tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat
     7381 tccgacacgt tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg
     7441 aatgcatctg cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt
     7501 aagaccaacc ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt
     7561 caaatctttc tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa
     7621 gaacgagccg tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt
     7681 gagattttcc taaaaaatta
//
LOCUS       DAJSMI010000123         7667 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJSMI010000123 DAJSMI010000000
VERSION     DAJSMI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.124"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            100..1074
                     /locus_tag="ON430_002133"
     CDS             100..1074
                     /locus_tag="ON430_002133"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR3220039.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            1244..2644
                     /locus_tag="ON430_002134"
     CDS             1244..2644
                     /locus_tag="ON430_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCR3220040.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(2722..3444)
                     /locus_tag="ON430_002135"
     CDS             complement(2722..3444)
                     /locus_tag="ON430_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3220041.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(3523..4029)
                     /locus_tag="ON430_002136"
     CDS             complement(3523..4029)
                     /locus_tag="ON430_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3220042.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            4152..5951
                     /locus_tag="ON430_002137"
     CDS             4152..5951
                     /locus_tag="ON430_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCR3220043.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(6019..7593)
                     /locus_tag="ON430_002138"
     CDS             complement(6019..7593)
                     /locus_tag="ON430_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3220044.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         2292 a         1548 c         1495 g         2332 t
ORIGIN      
        1 ttatctgtta tattttatag ccaaatagcg tgacatgggg cttgagaaat gttagcataa
       61 gcatgggaaa aaacaaatca atggaaggat tgatactaaa tgactgcagc tgcagaaaaa
      121 aaagatcatc aaaaagttat tcttgttgga gacggcgccg taggttctag ctatgccttt
      181 gctctagtta ctcaaaatat cgctcaagaa gtaggtattg tggatatcaa tactgctaaa
      241 actgaaggag acgcaattga cttgtctcac gctttggcat ttacttcacc taaaaaaatc
      301 tattctgcta cttacgcaga cgcacatgat gctgacttag ttgttattac agctggcgca
      361 ccacaaaaac caggtgaaac tcgtttagac ttagtaaaca aaaacctaaa aatcaaccgc
      421 gatgttgtca cacaaatcgt tgaatcagga tttaacggta tctttttagt tgccgctaac
      481 ccagtagata ttttgactta ctcaacttgg aaattctcag gtttccctaa agaacgcgta
      541 atcggttcag gtacctctct tgactcagct cgtttccgtc aagcacttgc ggaattagtt
      601 gacgttgatg cacgtaacgt ccatgcttac atcttaggtg aacacggaga ttcagaattc
      661 ccagtttggt cacatgcaaa tgttgccggc ttgcaaatct atgaatgggt aaaaaacaac
      721 ccagaaatcg atgaagaagc tatggttaac ctattctttg gcgtacgtga cgctgcttac
      781 acaattatcg agaaaaaagg cgctactttc tatggtatcg cagtagcatt agcacgtatc
      841 actcgtgcca tcttagatga tgaaaatgct gttcttcctt tatcagtatt catgaatggc
      901 gaatatggat tgaacgacat ctacattggt gcacctgctg ttatcaaccg tcaaggtatc
      961 caaaaagtaa tcgaaattcc tttaagtgac agcgagcaag accgtatggc tgcttctgct
     1021 aagcaattaa aagaaattct agatgaagct tttgctaaac tagacgctga ataatctttg
     1081 aacatgacgg aaaacatcta ttcggtgttt tccgtttttt tattttatca cccttatggt
     1141 ttctaaattt tttcataaag tagagataac cttcgtcttt tcctctaaat tatgctatca
     1201 tgagaccgtt ccaccctaaa cttttactta aggagttgtc gatatgacaa gatcttcgca
     1261 tcgaaaaaaa tcgaaacatg ttgctttgct ttcagttctt ggtgttctct tagtgctgat
     1321 cagcttttat acctatcgca gtacatacta cacaaagcat tttttgccga atacagaaat
     1381 aaatggaata aatgtcagca atctaacagt cgaaaaagca aatgaaaaat taaaagaagc
     1441 ttattctgat aaaaaactgg ctattaaaga agatggaaaa gtctggcagg aagtaccaaa
     1501 atccgagctc ggctacaaag atgatttcac ttcggagctt tctccgatcc taaatgaaca
     1561 aaacggatgg acttggggaa tgacttatgt ctcagcggca gaaaaacaag aaatagatcc
     1621 tgtcagtcaa aaccaccaaa aactagacac taccgttcag acattgacaa ctaagctgac
     1681 tgatttgaac aaagaccgga cacctactac tgatgcaacg atcgaaaaag caggtgactc
     1741 tttcgttatc aagcctgaag tcaatggaaa tacgatcgac gtcgatgctg cggtcaaaca
     1801 attaaaaagt gcggtaaact ctggtaaaga tacaattgaa ttgactgaat tcaaagaaaa
     1861 accaaaagta acatctgaag acagctcact gaaagaacaa ctcgcatcca tgaatgctat
     1921 cgcaaatgta aaagctactt acagtataaa cggtgagact ttccagatcc catcaagtga
     1981 tattatgagt tggctgacct acaatgatgg aaaagtcgat ttagatacag aacaagtacg
     2041 tcaatatgta acagatttag gcacaaagta taacacaagt acgaatgaca caaaatttaa
     2101 aagtacaaaa agaggagaag ttacggtccc agttggcact tacagttgga cgatccaaac
     2161 agacagtgag acagaagcat tgaaaaaagc gattctggca ggccaagatt ttactcgttc
     2221 acccattgtg caaggtggca caaccgctga tcatccattg atcgaagata cgtatatcga
     2281 agttgatctg gagaaccagc atatgtggta ttacaaagac ggaaaagtgg cccttgaaac
     2341 agatatcgtt tccggaaagc caactacacc tacacctgct ggagtcttct atgtttggaa
     2401 taaagaagaa gatgcaactt taaaaggaac aaatgacgat ggaacacctt acgagagtcc
     2461 ggtaaactac tggatgccga ttgactggac aggtgttggt atccacgatt cagactggca
     2521 acctgaatac ggtggagact tatggaaaac ccgcggttca cacggctgca tcaacacccc
     2581 accaagtgta atgaaggaat tattcgggat ggtcgaaaaa ggaacgcctg tattggtctt
     2641 ctaacaaaaa taatatataa aaagaaagca cccgtacgat tggtacaggt gctttctttt
     2701 tattatctaa caatgtgcta cttacatttc agcagcagta aagacttctg aaacatcatc
     2761 atcgtcttct agcttatcta ctagacgttc caattgctct ttttgttcct catttaaatc
     2821 aatcgtcgtt tgtggaatca tcgtcaattc tgcttgcgcc aaagtgaact ctttttccaa
     2881 ctcatctcgt acagcagcaa aatcttctgg tgcagtataa atctcgaata cttcaggaga
     2941 agtttgcaga tcttctgccc ccgcctctaa tacatcctca aacatcgtct cttcatcaac
     3001 agtcaaccca tcgcgttcaa tagcgatata tcctttgcgg tcaaacaagt aactgactga
     3061 acccgtttct cccagtgatc caccgtttcg agtaaaagct actcggacat ttgtcgctgt
     3121 ccgattacgg ttatctgtca atgcatggac taagattcct actccacccg gaccatatcc
     3181 ttcatacgtg atttcatcgt aattcgtttc atctacggca ctcgtagcct ttttaatcgc
     3241 ccgttccaca ttgtcgttag gcatatttgc agcttttgct ttgtccacaa caagccgcaa
     3301 agctggattc atagatggat caggaccgcc tgctttagct gccatataaa tttcacgaga
     3361 aagtttctga aagactttcc ctcgttttgc atcttgggca ttttttcttc cttggatatt
     3421 atgccattta ctatgtcctg acatgatttt tcctcccttg ttcttttgag aatacacttc
     3481 tattctagta ctatttcaca aaaaaacaaa gccacagcca atctataaac gcttcttgaa
     3541 tgccgcaaaa agcacacata atatcacagc agtcagagca ccgatcgaat ccaacatgac
     3601 atcttggaac ataggtgttc gtccaccggt catcatctga tggaattcgt ccaaacctgc
     3661 gtaccctgtt gctgaaagcc aagcaaggat cgctgtcagc caccaaattt ttagcttggg
     3721 gaaaacacct aaaaacaagc tgcctcccaa aataaaatag gtaccgaaat gtgcgccctt
     3781 ccttaaaaag aactctaaaa atttggcgta gcccagatgg tcgatactca ccatacttcc
     3841 accataatta aaacgtatac ccgctaatgt gtctttaaat ggctgattag gcagccagct
     3901 ttctaatcgc gaaacttgtg attgctgtcc gtaagtttga gaagaactgt aaaataagat
     3961 tataacgact atcagtgcga ttactaaata aatatttccg tttttcattt gtttttttga
     4021 agaattcata agtcacctct gtattttatc ttagactttt tttgaagaaa caacaatcat
     4081 tttcatagag acttcttcca taaaaaagtg ctatacttaa ttttgtatta caatatgaag
     4141 ggagtttttt catgagttat tcattaaatt ggaatctaga ttctattttt tccggaggca
     4201 gtcattctga tgctttgaac caacggatga agcaattgga agaccaaatg aacgagtatt
     4261 atcaccgcgt aacaaaatgg tcacctagtt ctgacaatgc agagcagtta aacgctattc
     4321 ttcaattgca agaaacgatt acgaatggtt ttacccaatg cagcagctat attactgcat
     4381 tgctttctgc caatgtcaat gattcagatg caaaggtctt atcaggcaag ctttacgcaa
     4441 tgctgcctcg tctgcaatca gccgaaactg tattgtcaaa gaaatttgca gaaatatctg
     4501 acaatgattg gaatcagatg ctttctcaat cttcgtttga aacaattgct tttcgtttaa
     4561 acgagattcg tcgtgatggc tcacaattat tatcagaagc tgaagaaaac attataaata
     4621 cgctttcttt agatggtcta aatgcttgga gcagtcatta tgatacgatt gtagccagca
     4681 tttcgattcc cttcgaacaa gatggacaag tagtcgaatt atctgctgga caagccttca
     4741 ataagatgat gggcgatcca gatccaaaag tcagagaaac acttttcgca gcatgggaag
     4801 aagcctggaa agaaaaggca cccctcttca cggatacttt gaatcattta gatggtttta
     4861 gactttctga ctatgaactt catggtgtca cagattttct acaaaaacca ttagagtaca
     4921 atcggttgaa gaaagaaaca ttgtctgtca tgtgggatac aatccagaaa aacaaacaac
     4981 cccttgttaa ttacttgact agaaaagcta atcttttcgg taaagaaaaa atggaatggc
     5041 aagatcaaga tgcacctatt attttaggcg atttgaaaga aaaaaccttt agttttgacg
     5101 aagcagcagc atttattatc gaaaacttta acaaattcag tccaaaaatg gctaaatttg
     5161 cccaatctgc ttttgaaaag agctggatcg aagcagaaga tcgtcctggg aaacgtcctg
     5221 gtggttattg tactgagtta ccagaaacaa aagaatcaag gatctttatg acctacagta
     5281 attctgttaa tgaggttgcg acacttgctc atgaactagg acatgctttc catagcagca
     5341 cgatgtggga tttaccttct ttaaatcgcg aatatgccat gaatgtcgca gaaacagcaa
     5401 gtacattcgc agaactgatc gtagccgatg ctacattaaa agtcgcaaaa acaaaagaag
     5461 aaaaaatcaa tttgctagat acaaaactgc aaaatgcttt agctatgttc atgaatatcc
     5521 attcccgatt catttttgaa aatcgattct atgaagcacg gcaagaagga ttagtctcag
     5581 aagataagat cacagagatg atggttgagg cgcaaaaaga aggctatcaa ggagctttag
     5641 ctacttacca tccatatttc tgggctgcaa aattgcattt ctttattgat gacgtaccgt
     5701 tttataactt cccatacaca tttggctacc tctttagttt gggcatctat gcttacgcca
     5761 accaaaaggg agcaaatttt gaagaagaat atatccattt actacgcgat actgcctcaa
     5821 tgacgacgga agatcttgcg aagaaacatt tgaatgtcga tcttacccaa ccagattttt
     5881 ggcaagcagg tatcgacatg gtgactgaag atatccaaac gttcatggaa ttgacagaag
     5941 aatttatctg atttctattt tatcacaata aaaaaagagt tagtccagaa tgggctaact
     6001 ctttttgatt aatcattctt acatgctgac agcaaagtca ggggcaaacc attgaactga
     6061 accaactttt acgctttcgc ctggttgagg agcatggata tattgtcctc cgcctaaagc
     6121 aatcgctacg tggtaagttc cgccttgtga cccccagaat aacaagtctc cagcttttgc
     6181 ttgagaaact gaaatttttg ttccagctga ttcttgagga actgtccaac caccgatgtc
     6241 acgaccagtt acttgcatgt aaacatagcg tgtgaaccct gagcagtcaa agccacttgg
     6301 atctttaccg ccccaaacat atggagtacc aatatatttg taagcttctg ctacgattgc
     6361 tgcaccattc acacttccag aaggagctgg agcaggtgtt acaggttttg gttgttcagg
     6421 tgttggtgtt ggcgttggag ccggagtact acttcctgtg ccgtttcctg tatcaacact
     6481 ttgatctgtt gatggtgttg taggcgcagg tgttgttgat tcttctgttg ttgaagattc
     6541 aggcactgtt gttgattctt ctgttgctga agattcaggc actgttgttg attcttctgt
     6601 tgctgaagat tcaggcgctg ttgttgattc ttctgttgct gaagattcag gcgctgttga
     6661 ttcttctgtt gaagattcag gtgttgttga ttcttctgtt gctgaagatt gtgtagctga
     6721 tgatgattct tcagtcattg aagattgtgt agctgatgat tcttctgttg ctgagctttg
     6781 tgcagtagat gaagcttgag tagcttgtgc ttcagcctct gcttgttcgc gtgcttcttt
     6841 ttcagctttt tcttgtgcag cttgttgacg agcttgttct gctagacgtt gttgttcacg
     6901 aatacgagct tgctctgctt cagcttctgc tttttgacgg ttcaagtcag ctttcttgtc
     6961 ttcagcagtt gcttgttcag cagctaatga agttttcagt acgttcaaat cagcttgttt
     7021 agataaaaga tcgccttttt gagattccaa agcagcttgg ttttcagcta attcttttag
     7081 tttagcatcg ttttcagctt ttttgtcttc tacagctttt ttatcttgtt tttgttgttc
     7141 catcaagtcg ttgtttgctt ttaccatcgt tgtcattgct tgaacgcgtc caatagcatc
     7201 tgctaatgaa tcagcattca atacagcatc gatgtagttt gagcttgtgt tgcttacttg
     7261 tgcttcgcgt gcttgttttt ggattgtatc ttcacgtttt tcaatacgtt cttgtaaatc
     7321 tgcaatgtct tttactaatt gtgcagattc ttgacgcaat gtatcttgtt ttgctaatag
     7381 gtcttgtgct tgtgtgttga tttcagaaac ttggctttct aaggcatcga tttgtgattg
     7441 tgcatctgct tgttgatttt tcaagtctgc aatcttttga tcttgctgtt gtatctgaga
     7501 atcaaaatcg tccgccgcgg cagcgatcgg tgatgctacg gcagtcaagg tcattgaaca
     7561 taccattact gctgatatta aactcttttt cactcttcat tcctccgact ggcttaattt
     7621 aataaatatc taaatatatt taagtctttc ttaacgatat aaaaatt
//
LOCUS       DAJSMI010000124         7572 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJSMI010000124 DAJSMI010000000
VERSION     DAJSMI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.125"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(105..629)
                     /locus_tag="ON430_002139"
     CDS             complement(105..629)
                     /locus_tag="ON430_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3220045.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ON430_002140"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ON430_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR3220046.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ON430_002141"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ON430_002141"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR3220047.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ON430_002142"
     CDS             complement(2351..3031)
                     /locus_tag="ON430_002142"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR3220048.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ON430_002143"
     CDS             3157..4026
                     /locus_tag="ON430_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3220049.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ON430_002144"
     CDS             4052..4567
                     /locus_tag="ON430_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220050.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="ON430_002145"
     CDS             complement(4614..5207)
                     /locus_tag="ON430_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3220051.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ON430_002146"
     CDS             complement(5232..5411)
                     /locus_tag="ON430_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220052.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ON430_002147"
     CDS             complement(5488..5997)
                     /locus_tag="ON430_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220053.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ON430_002148"
     CDS             complement(6093..6473)
                     /locus_tag="ON430_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3220054.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..>7572
                     /locus_tag="ON430_002149"
     CDS             6803..>7572
                     /locus_tag="ON430_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCR3220055.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHALNTYKRR"
BASE COUNT         2147 a         1571 c         1344 g         2510 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgctc taaacacgta
     7561 taaacggcgg aa
//
LOCUS       DAJSMI010000125         7487 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJSMI010000125 DAJSMI010000000
VERSION     DAJSMI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.126"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            23..1315
                     /locus_tag="ON430_002150"
                     /pseudo
     CDS             23..1315
                     /locus_tag="ON430_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1834..2568
                     /locus_tag="ON430_002151"
     CDS             1834..2568
                     /locus_tag="ON430_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3220056.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            2584..3753
                     /locus_tag="ON430_002152"
     CDS             2584..3753
                     /locus_tag="ON430_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3220057.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            3769..4764
                     /gene="lacD"
                     /locus_tag="ON430_002153"
     CDS             3769..4764
                     /gene="lacD"
                     /locus_tag="ON430_002153"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR3220058.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            4843..5769
                     /locus_tag="ON430_002154"
     CDS             4843..5769
                     /locus_tag="ON430_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCR3220059.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            5788..7038
                     /locus_tag="ON430_002155"
     CDS             5788..7038
                     /locus_tag="ON430_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3220060.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDANRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
     gene            7257..>7487
                     /locus_tag="ON430_002156"
     CDS             7257..>7487
                     /locus_tag="ON430_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3220061.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEK"
BASE COUNT         2472 a         1256 c         1529 g         2230 t
ORIGIN      
        1 aagatcaaga ggtgattaca cgatggctag aaagaaaaga aatcctgatg ccgaaaagtt
       61 agctgaatcc attctgaatg cctatcaacc tgaatctgtc gatgacatgc aagatgcttt
      121 gaaagatgtg tttgggcccc tttttgaaaa aatgcttcaa ggagaattga ataatcattt
      181 aggttatgat gcccattcta aagagcctaa ggaacacgat aaccgtcgaa atggctatgg
      241 aactaaaacg cttaaaacca gttttggtga agtagctatt gatgttccta gagaccggga
      301 agcttccttt gaaccagagt taattcctaa gagaaaacga gatgtctccg acatcgaagg
      361 gaaggttctt tccatgtatg cacgaggaat gagtcaatga gatattgccg caaccgtcga
      421 agctatttat ggctttgata tttcccatga aatgatttca gatatcactg acgctgtcct
      481 tcctgaattg gaagaatggc aagcccgccc cctagcaaag tgttatgcct ttctattcgt
      541 tgattgtatg tatgttactt tacgagaaaa ttatgaagcc aaagaatatg ctgtacacac
      601 cattcttggc tatgatctca aaggaaataa ggagatttta ggattatggt taaatcaaac
      661 agaatctaaa aatcgctgga tgcaagtatt tgatgaactg aaagcacgtg gtgtcgaaga
      721 tgtctttttc atttcgatgg acggtgtttc tggcctcgaa gaaggcgcaa aagcgatctt
      781 tccatcggta attgttcagc gctgtattgt tcatcttgtt cgaaatgcat tgcgctatat
      841 tccaagtaag gactataagg aagtctgccg agatatgaaa aagttctacg gtgcttcttc
      901 tctaaacgct gcacatgctg cttttgacag ttttcaaaat cggtggtctc attattctgg
      961 tgctgtagat gtctggaaac ggaactttgc acatgttgaa caactatttg attatggtag
     1021 tgccattcga aaaattatgt acactaccaa tgcggttgaa agtgtccact ccagcttcag
     1081 aaaagtcaca aaaaaaggag cgttctctaa tgagaatgca ctcctaaaac tactttattt
     1141 acgcacgaaa gaattacacg cgaagtggtc aggtggtcga attcaaaact gggctatggt
     1201 gcttaatcag ttaatgatca acgagacctt ctcctctaga atgaaaagta tgcgatttac
     1261 cttccgtaac aacggtaggt ttattcaact atgtgtccga tcaaaattta tctgatttca
     1321 aaatagccga gagaattatg gtggttaaaa actcttaact atttaacttc cacagtttag
     1381 ttgacaaacc cacttaattc tgataacgaa aaacatattc gaatgattaa acagaagtaa
     1441 atacttattt aaataacttt taattttcat gttcataaat gatttgaaac caaagcaaat
     1501 gcgttgcact tttcctccgt cttccgaaaa tcgttcaatt cactggtaag tagtcatttg
     1561 atagtttgag gcaaattgtt gattgatgaa gggtaggagc taaaaaaaga taccagttga
     1621 agagagaaaa agaaaaaaat agagggtgtt cttgaatgaa aaaagttgga aaattatttt
     1681 tcatttcctt ttattcctat taaaatcagt gatgtcaatc atattaacaa gactaaaaat
     1741 gaaaataaaa aaaagattgt tcttagtatt gactcataaa aaataccact actatactta
     1801 cattgtggtt atagccagtt aggagagaca tcgatgcaaa atacctttca atcaaaatca
     1861 ctttattatc agctggtaga aatattgaaa caacgaatcg aaaacagtat ggtcgctcat
     1921 gacaagctgc catcagaaag agagttgacc agtttttatg gcgttagtcg cacgacggtg
     1981 cgactagcat tgcaagaact ggaaaaaatg ggctacattt atcgacagca tggaaaagga
     2041 acctttgttt cggaattaag gacaaatgcg actgatctag ctggcgcata tagttttacc
     2101 gaacaaatgc gggcaatggg caaacatcct gaaaccaaga tattggcctt caagaagatt
     2161 gaagcaacta aatatttagc gagtcatctg aatgttcaac taggtgagcc tttatttcaa
     2221 ttagaccgat tgcgcttagc tgatgatttg ccaatgatga tcgagaaatc ctatttgcca
     2281 gtgaagctat ttatgtccct ttctcaagaa attctatttc tgaaaccttt atataatttg
     2341 ttttcagaga atttcaatca aatcgtacgg ttggctgacg aggaatttta tgccagcatt
     2401 gtccagaaag aagatagtga gttgctaaat attccagaag gatcaccagt gctccattta
     2461 gttcgctcaa cctataatct cgagaacgaa gtgattgaat ttacattaag tgtagcgcgg
     2521 gctgataaat ttcattataa gatcaggcat ctgcgttcca ctaactgaat tggaggaaag
     2581 aaaatgttta tgttctcaaa agaagaacta cgaaagctag gagcagaaaa tactacccga
     2641 gaaattcgac aacaaccgga actgtggcat gagacgtttg accgttatca agaaacagtt
     2701 gcagatttag atcgtttgtt tacggatgcg aaacaacatg ctggagagcg accaatccga
     2761 gtcattttta caggggccgg aacctcgcaa tatgtgggag atacactaat tccgtttctt
     2821 actagtcaag gaaatacgaa ccagtttatt tttcaaagta ttggttcgac cgatatcgtt
     2881 gctacgccaa aagaatacct gattcctgat gagacaacga tcttggtctc atttgcccgt
     2941 agtggaaata gtccagaaag tctagccgca gttaagatgg ccaatcagct ggtcaacaag
     3001 atctatcata tcgtgatcac ctgtgcacca gacggattat tggctacaca aatgcctaca
     3061 aaaaaagaaa atctagtttt actaatgcca aacaaagcca atgacgctgg ttttgctatg
     3121 acaagcagtt tcagctgtat gacgctgact gcgctactta tttttgatca agcaagcaat
     3181 cagcaaaaag aaagatatgt tcatcaatta agccgactag gagaagaagt catccaacga
     3241 gaatcagaga tccaagggta tctggcatca gactttcatc gcatcattta cttaggttcg
     3301 ggaagtttag gcggattgac tagagaagcg caattaaaag ttctcgagtt aactgctgga
     3361 aaaatcgcaa ccgtatttga tacatcttta ggattcagac atggtcccaa atcttttgtc
     3421 aatgagcaaa cattggtttt cctgtttgtc agcaatgact catatacgca gaaatacgat
     3481 gtagacatgc tggaagaaat caaaaatgac ggtattgccc aacgcgttgt tggaattggt
     3541 caaaagggtt cattgtttag tggtgattcc tttgaattag ttgcagattc gttactgccg
     3601 gatggctatc ttgctttagc agatatcatg gtagcccaaa cgatcgcatt actttgttcg
     3661 gtgaaggttg gtaatacacc agatacacca tcgccaagtg gaacagtcaa tcgtgtggtc
     3721 aaaggcgtaa cgatttatcc atatgaaaaa taggaaagag tgtgaagcat gttagaatgg
     3781 tcggaaaata aaagacaagc aatggaacgt ctatcgacgc cagaaggcat tattaccgct
     3841 ttggcgatcg atcagcgagg cgcgctgaag aaaatgatgt cggctttagg gatggaagcg
     3901 acaaaggaaa aaattgagca gtttaaaaca ctaatttccc aagaattgac tccttatgcc
     3961 tcagcaattt tattggatcc tgaatacgga ctgcctgctg cacagaagcg tgcagcagcg
     4021 agcggtctgc tgttggcata tgaaaaaact ggttatgatc aaaccgtacc cggccgcttg
     4081 ccggatctgc tgtcggactg ttcagttctg cgattgaaag agttaggtgc ggatgcggtg
     4141 aaatttttac tgtattacga ttatgaagaa gcagaggaaa tcaatcggca aaagcaagtt
     4201 tttgttgaac gaattggaag cgaatgcatg gctgaaggac tgccatttta tttagagtta
     4261 ttgtcatatg atgctaaaaa tgtagatacg aaatcgcttt cctatgccaa agcaaaaccg
     4321 cgcaaagtga tcgaaatgat gaaagaattc tcgaaaaccc agtataaagt agatgtctta
     4381 aaggttgaag taccagtaaa catgtcattt gttgaaggaa atgcaacagg ggagatcgcc
     4441 tatacacgtc aagaagctgc cgcttatttt aaagaacaaa gcgatgcaac agatttgccg
     4501 tatatcttct taagtgctgg ggtatcagcg gcattattca tggagacatt gcgttttgcc
     4561 aaacatgctg gggcacgatt caacggtgta ctctgtggca gagcaacttg gaaagatgca
     4621 gtcgctcctt ttgttgaaaa aggagaagcg gcaactttag aatggctgac agaacaaggg
     4681 acacaaaaca ttcgtcaatt gaatgaagtg ataagagaaa ctgccatccc atggtatgag
     4741 aaagtaagtg aaagcacttg ttaatgtttc taatatatta gtagtgataa gagatatgtg
     4801 tgattataat ttatcattat tgaaactggg aggatatttt atatgaataa aagattgtta
     4861 gattattcag cttcagattt tttgaaagct aagccgatgg aattaaagca ggcaattttg
     4921 gcttctgaag gtagaacaat tatggcagaa aatgtgccaa gtagctgtcc ttacatggga
     4981 aaatcagtga caaatgcaga attggagaga gctgctggag ctgatttaat tttgtttaat
     5041 gcactagatc tttttaatcc tcaaatttca ggtgtacctt taactctaaa agagaatcca
     5101 ataatctgga ttaaaaaagc tttaggaaga gctattggtg taaatttaga acctgtagac
     5161 aaagaagttt tgatgagtga aaatcgaact gaaatttcaa gtggtagaat cgtttctcct
     5221 gagactttaa aaaaggcaaa tgaattaggc tttaatttta tttgtttaac tggaaatcca
     5281 ggaacaggag tatctaatag aactattgag aaagcaataa cattatccaa atcaattttt
     5341 aatggattaa ttatcgctgg aaaaatgcat ggtgctggtg ttgatgaacc aattatgaac
     5401 ttagatatag caaaaagatt tattaaaaca ggtgtagata ttttattagt tcctgctcca
     5461 tacactgttc cgtatttcat ggaaagtgat ttgcgaggaa tcgttgatta tataaaagat
     5521 tttaatagaa ataaatctat tgaggaaaaa gttctagtac taacggctaa tggaacaagc
     5581 caagactctt ctgaccaaga aactattaaa aaaattgcat tagcatcaaa agcatgtgga
     5641 gctgatattc aacacatagg ggattcttta aatggtattt gtcttccaga aaatattttt
     5701 gctttgggtc aagcaattcg gggatatcgt catcaaatta ttatgttagg aaaatctaat
     5761 tatcgttaat taaggaggaa atgaaagatg atcgacaaag ttcaaaaaat agctgcaaaa
     5821 attagtaatc aaagacatct actagcttta agagacagca tggctgcagt tatgccatta
     5881 atcattattg gttccttttt tatgttatta gcaaatttcc cgatagaagg atttacaaaa
     5941 tggttatctg aaattggagt acaatccttt ttgaacaaag catcagattc aacatttggt
     6001 attattggat tagctgttac ctactctttg ggacacaatc tagcaaagac ttatggtgta
     6061 gactcattat cagcaggatt attatctttg agtggatatg ttttacttac acctttattg
     6121 gatgcagatg gttctatagg atttccaaca aaatactttg gtacctcggg tctttttgtt
     6181 ggtattattg tagcacttgt atctacagaa attttccggt ggtttgtact taaaaatatt
     6241 acaattaaaa tgccggcaag cgttccacca aatgtaagcc gagctttcgt tgctattatt
     6301 cctggctttt ttgtcgttct actatggttt attgttgttg taatttgtta taaacttgga
     6361 attgaaaatg ttcatgctct tattgcagat acactcgcaa aaccactatc tttactgacg
     6421 aaaactttac caggaattat attggttata cttattcaat gtttcttttg gatgtttggt
     6481 attcatggtg cacaggtaac ggggccaata attgaaccgt tattattgca aaactctgat
     6541 gcaaatagaa ttgcttatca agctggaaaa gaacttccta acattataac ttatgaattc
     6601 ttatataact ttgttttttc tggtggtgca ggttgtttat ttgcacttgc cattttaaca
     6661 tttttctttt caaagagtca ggaaaataaa actttaggta agttatcaat cgctccagta
     6721 agttttcaag tagcagagcc ccttctattt gggtttccaa ctatattaaa tataaaaatg
     6781 ttaattccat ttgtaactgc accagtagta acaacattaa ttacttattt ttcaatgagt
     6841 atgggattag ttgcaaaacc tgtaggagca acaatcccat ggacaactcc accgataatt
     6901 gccggatttt tagcttctgg tggacgaata agtggtgcag tgattcaagt aataacaatt
     6961 gcaataaatg ttttgatata ctatccattc tttaaattag atgataatgc caagctaaaa
     7021 agcgaaaaaa atgattagaa ctacctatat gtttctaact gtagttaagt atgaaaagac
     7081 tatttttaaa tttagcattt ttgcgctatc ggcgtagatg aagtatcacg ataacgaaat
     7141 tagtttcttt catttagaat cgccgtttgt ataattaagc gagacaaata aaaaagccat
     7201 ttatgttaca ctcaaattgt aatacccgct aaagcataca aggagagtga acataaatga
     7261 cttataccca tcttacacca aacgagcttg taatgataga agcatatttt catcaagaaa
     7321 ctccggttgc tatcgttgcg aagcagctta aacgtggacg ccaaacaatt tacaatgtct
     7381 ataactttct caaatgtggt ggaacagcac ttgaatactt tgaacaatac aaagaaaata
     7441 agcgacgttg tgggagaacc gaaatcattt ttcctgctga ggaaaaa
//
LOCUS       DAJSMI010000126         7412 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJSMI010000126 DAJSMI010000000
VERSION     DAJSMI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.127"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..1290)
                     /locus_tag="ON430_002157"
     CDS             complement(<1..1290)
                     /locus_tag="ON430_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3220062.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDP"
     gene            complement(1301..1969)
                     /locus_tag="ON430_002158"
     CDS             complement(1301..1969)
                     /locus_tag="ON430_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR3220063.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2100..2684)
                     /locus_tag="ON430_002159"
     CDS             complement(2100..2684)
                     /locus_tag="ON430_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3220064.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3015..3248)
                     /locus_tag="ON430_002160"
     CDS             complement(3015..3248)
                     /locus_tag="ON430_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCR3220065.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3263..4210)
                     /locus_tag="ON430_002161"
     CDS             complement(3263..4210)
                     /locus_tag="ON430_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR3220066.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4210..5052)
                     /locus_tag="ON430_002162"
     CDS             complement(4210..5052)
                     /locus_tag="ON430_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3220067.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            5374..6561
                     /locus_tag="ON430_002163"
     CDS             5374..6561
                     /locus_tag="ON430_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3220068.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            complement(6653..7393)
                     /locus_tag="ON430_002164"
     CDS             complement(6653..7393)
                     /locus_tag="ON430_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3220069.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
BASE COUNT         2419 a         1355 c         1041 g         2597 t
ORIGIN      
        1 cggatcatga gtggctataa ctactgcacc cttttctttt aagtctttaa taaggtcagc
       61 aatcctttcc atattgatat aatccaaccc acttgtaggt tcatcaaata gaaataaatt
      121 agtttttgac aatgaagcta tagaaaccaa taatctttgt ttttctccac cggataaatc
      181 aaacggtaat tgatctttca ttttataaag ttccattttt tttaaaattt tttcttgctg
      241 atctttacta actatttcat tccccagggc taactcgctc ctcactgatt ctgtgaaaaa
      301 ttggtaatcc atttcttgca ttactaaaaa cggcgatgac aaattttttt tattttcgat
      361 tatctttcct ttggtagggc gaataatctt catgagtatt cttagtaagg ttgttttccc
      421 cacaccgttt ttccctacca aaccaataac atctccaact ttgacatcaa aatttatgtc
      481 ttttagaata ttttcataag taatttcttc aacttttaat acatctgcac ttaccttttc
      541 gatataggga acttcaatcg aactattaaa aatgtcaaat ttccttaatg cgttattgtt
      601 ttcagttttt aacaactgaa tagttgtagt ttttacagtt ctatttttaa caaatagaac
      661 ttgatcaatt aattctttca aatagtaaaa acgatgttca gctataacaa tagtaaatcc
      721 tttatttttt aaatcacaaa gtaagtcagc aagtttctta gtcgctttga agtccaaatt
      781 tgaagacggt tcatccaata taattaattt agagtttagc gataaactac atccaataga
      841 aattttctgc cgttcaccac ttgatagcga aaaaatgttt ttatctaata tatttgaaag
      901 atctaataat tcagctaatt cattgattct tttatccata tgctcttttg taaatccaaa
      961 gttttccatc gaaaatacta actcactcgt tgtatttgtt gtaaagaatt gtcctctagg
     1021 attttgactt acgtagccaa tattttttga tatactttct tgtgttaagg attgtagcga
     1081 gttattttca aataaaattt ccccaaccaa ttttccgtca taaagtcttg gaataagtcc
     1141 attcattaac tttaatagtg tggatttccc actcccagaa gaaccagtaa tcaatgtaag
     1201 actgcctttt tttattgaaa aattaaggtc aacaagcgct tgttccttaa catccttata
     1261 tgaaaaagaa acattgttaa attcaaacaa ttctttcacc tcaaatcaaa atacttaaga
     1321 aaaaaatagt aacggaaaac aggatgatat agtctaataa gctcatgttt gttttatagt
     1381 aagaacttct tttcacagga gattctatcc cctttgttat tgcattagcg gtcatttctt
     1441 cagcaatcat agttgcacgt ataagaacgg gaactaacag aaattccaaa tattgcagag
     1501 gctgaaaaaa agacaagcga atgtttctca tttttaaaga ttctttaatc attcttattt
     1561 cagaacgata agtcggaaag aatctgatca ctactgtaat acttaacatt atttttttcg
     1621 gtaaatgtat tttttctaaa gaggataata attcattgct agtatagaca ctaaccaaca
     1681 accatgagat cattacgacc ggcaacattc gaaatacaat atataaggat gtcccaaaaa
     1741 atatagtaaa aaactctatc ttgatagcat caataatttt cattactaaa aacagaactg
     1801 aataaatcaa gatgaaataa atacatgtct ttattttacg aaacatcaat actaaaactg
     1861 tagcaataat gaaacacccc acttgaaata tatttccctt tcctaaaaaa ataatgaccg
     1921 gaaaaaaaat atttaaagta atcaaggtta acggattgat ttttgacact ctttcacctc
     1981 caaaaaataa gatcgcactg ttatcttaag ctgacaaaaa tgcaccgtta ttcagataac
     2041 agtgcgatct tatcaaaaag cataactgag aacaattctc aatgcaagct tttttttaat
     2101 taactcagta attcaatcca cccaggaaca gaaaattgat acaatcttct ggttaatagc
     2161 atagtctttt cttttgaata gctatgcttc aatatatcat agaatgtagc gtagaatgag
     2221 taaatgtaga agtggatttc ttcttcatta aagactaaat tttgattgat cattccgata
     2281 gtctttaact tatctagaca acagactgtc ttttttgtca caaatgatgt aattttttca
     2341 acaatatctt gatatttggt tccagctgat tggaataaaa ccaaatcgaa tatacgttta
     2401 ttctcaaaaa tataattaat aaatggatct aattcattga agttctctct ccaacttttc
     2461 tctatatcta tattttctaa gtttaagagg tatttggtaa actcttcatt atacatatta
     2521 ataagcgcat taatatgcgg ctgaataatt tcttcaaata acgcttcctt agaagtaaaa
     2581 tgtttataaa aagcacccgt agttacgcct gcggtcaagc acagttgcct tatcttagtt
     2641 tgctgaaatc cttctttttc aaaatggctt agtccacttt ctattaattt ttggtgagtt
     2701 gtaatataat ctctggtttc tatgggtact tcctcctaat aagatgaatt aactatatta
     2761 tcttacacag gacttgaagt ttcaatcgtt aacgtctatt actgaactca ttccttagag
     2821 ctaaactaat gagaaagagc aaaataactg atacaaaaag caatcctaca agaattaata
     2881 gactcatcta aatcattcac cttttctaaa ataatccgag aaagatttaa aaaaattata
     2941 tccagctatt agggtaagtc ctatcataat cactccaata gccattttct tatcctccct
     3001 aaaaataaag tcttttactt aaaacctttt ttgtaatcgt caataaattt tttcccttta
     3061 tcagttaatt gaaatcctat atctaaatag tcctcatatc taagtttagc cagcatctca
     3121 cttacagatg ctggactaac acttagaact tttgctaaat cattattttt taatttcttt
     3181 tttctttttg ataattcctg aattgctagt aaataaatta gtatagtagt cgttttaatt
     3241 tgtaccatac ctaccctcca aattatttcg aaaggccttc agcaatttta tccaggttcc
     3301 atttcatcat cgcatagtaa ctatcaccat cttgtccttt ttctgcaatt gaatcagtaa
     3361 aaatcgttga atagatagga atattggtat cttttgatac tgttttcatt ggtctatcgt
     3421 ccacactgct ttctacgaat aaggagggaa cttttgttgt gcgtaatttt tcaactaagt
     3481 gttttatttg atctggtgtt ccttcttctt cagtgttgat ttcccaaatg taagcagaag
     3541 gcacattata cgctttcgag aaatatttaa agcatccttc acttgtaaca atcatttttt
     3601 tatcattcgg aattgaagca aatttagatt tagcttcttt gtctagagaa tccaattttt
     3661 caatatactt atctagattt tctttataga attttttatt atcaggatct ttttccgcta
     3721 actgtttttc aatattttta gcgtaaataa taccgttttc taaatttaac caagcatggg
     3781 gatcttcctt ccctttctca ctctgaccct ctaagtaaat aacatctatg ccatcacttg
     3841 ctgcaaaata gtctttgttt tcctctttgt tcgcattttt tactaatttt gtaaaccaag
     3901 catttcctcc agtttccaag ttaacaccgt tataaaaaat caaatctgct tttgaagttt
     3961 tttgaacatc ttcaggcaaa ggttcatatt cgtggggatc tttcccaata ggtacgatac
     4021 tgtgtaaatc aattttatct tttgctatat tttcagtaat atctgctaaa atcgaattag
     4081 taactactac ctttaatttt tcgttcgaag ccactgtatc tttgtcttta ctatttgttg
     4141 aacatgcccc gaatataaaa agagcaatca aaatccccag acttcccagt actgctttcc
     4201 attttctcat cataactttc tctccttctt tcttaaaaat ttttgttttg gtgagattag
     4261 aaaactaatt acaaataaga atgccgctgt taaaacaata cttgatcctg ctgctatgtt
     4321 aaaactataa ccaatgaaca aacctagcaa tgatgagcac gctccgactg tcgccgaaat
     4381 aaatatcata gtttttaagc tgtttgcata taaatatgct gtagcagctg gtgtaattaa
     4441 cattgcaaca attaatatag ttcccacact ttgcatagct gttactgcta ctaaagttaa
     4501 tagaatcatt aatagataat gataaaaact gacattcatc cccattgctt ttgccattaa
     4561 tggatcaaaa gaagtgatta ataattcttt aaaaaataga acaactgtaa ccaacactaa
     4621 aacagaaacg ccaattgtaa tccacttatc aatatcttgt acagctaaaa tatttccaaa
     4681 taagatatga aataaatccg ttgagctatt tgcaactcct ataaggataa ctcctaatgc
     4741 taaaaaagaa ctgaaagtaa taccaattgc tgtatctcct tttattacac tattgctttt
     4801 tataaaagta ataatgatag aagctaatag accaaataca atcgcaccaa taaaaaaatt
     4861 aatacctaaa atataagaaa ttgctactcc tggcaaaaca gcatgagaaa tagcatcacc
     4921 cataagagac attcctctta aaataataaa acaacctact gttccagaaa caatccctat
     4981 aactacagat gtaattagag cattctgtaa aaagtgaaag tcatacagac cattaataaa
     5041 attttgaagc atattattcc cccttgtata ttagagttag ggttttaacc ttccggtttt
     5101 gattggataa ccctatgaac catcgttatt ttaatttggt attaactcat ttcttttgag
     5161 ataatatatt tattggattg agaggctaag ccgttccccc cttgggccat tgaacccgca
     5221 ctcgagagac tggctcctct ctctgataag tggattgcgt atgagttaga tgttgaaagt
     5281 ccttacttta ctccgtatct cgtacaaggt agtgacgctt ttcagttgag tggatttcaa
     5341 tccaaaattc tttttttgaa aggtcagata tacatgtact ttttaggcat tgatatcggt
     5401 aaacgcactc acgttgcttc tatcatgaac gaggaaggaa aggtattact aaaaggtttt
     5461 tcatttccta acaccactga aggagcagag tcattaattg agcgaatggt cgactattcc
     5521 ggagcaccca gtgactttgt tatagggatg gaagccacgg ggcactattg gctgtccatc
     5581 ttttcatacc ttcacgaatc agattatttg attcacgttg tgaatcccct tcaaacagac
     5641 ggatggcgta aaggtacaga aatacggaaa cgaaaaaacg atattattga ttctgttttg
     5701 attgctgact taatgcgcta tggctcattt gtcgaaacgg tcttatccga tgaaaatgtt
     5761 ttttctttga aacaactttc tcgttaccga acttatttag taggtacagc gagcgacttc
     5821 aaaagaaaaa ttatagccgt attggatcaa gtctttccag aatacgcgac gatttttacc
     5881 aagcagggcg ttttcggaaa agcttctaaa gagttattgc tggcgtttag tgccccgatc
     5941 gacttagaaa acatttcctc agaaactttg gcccaatact tagctgagtt aagcaggaac
     6001 cgaatcaagg cagaaaaagc ggaccaattg aagcaagcag cttctaactc ctttggagta
     6061 aaatttgctc aagaagcgtt caccttccaa ctacgttcaa tgattgaaca attaaaattc
     6121 attgaaagcc aaataaagga aacggaaaga gaaatcaagc acataatgga cactttaaat
     6181 tctgttattc tcacaattcc tggaattggt cctataaacg gtgcgactat tcttggtgag
     6241 atcggagata ttcaaaaatt ttctaacccg aaaaaactcg ttgcttacgc tgggatagac
     6301 gcttccgtga cacaatcagg gcaatatgag gcaacgcata atgttatgag taaacgaggg
     6361 tctccatatt tgcgaaaagc cttgttttcg gcggctttag tagcttctca atgtgatcct
     6421 gtttttagtg cgttttacca gaaaaaagtt tccgaaggaa aacatcatct aactgcctta
     6481 ggtgcggttt ctcgtaagct ttgttacgtg attcatgcta ttttgactaa aaacgagcct
     6541 tatataattg tttctgatta gctattgaac attcaaaaaa actaatggta agaagcttat
     6601 ttgttgtacc cttttttcgg aattatctgt tataaaatat tttttgattt ggctaatttt
     6661 tacttgactc tatatagtta gtctctccaa taaatatagt atctccatag gcttttttta
     6721 aattctcttc attaaataca gattctacac ttccgtgagc aattagattt cgattcaaaa
     6781 gaataatatt atcaaaatac ttctttacct tgcttaaatc atgatgcaca attagcactg
     6841 tttttccctg atgtttcaat tcttttaacg tatccataat tattcgttca ctaactaagt
     6901 ctatccctac aaatggttca tctaaaaata tgaaatccgc attttgagca agacatcttg
     6961 ctaaaagtac acgttgaaat tgtcctccag ataattctcc tatttgatga ttagaatatt
     7021 ttcccatatc gaccttttcc aaagcctttg aaactctttg ccattctgca tttttaattc
     7081 tttgaaaaac tttcattccg gcatatgttc ccatagatac acattctttt acttttattg
     7141 gaaaagtaaa atcaatatca gctttttgct ctacatatgc aatcttcacc agttttttat
     7201 tgatatcttc attattaatg gagacctctc cctgatgagg gataatattt aacattgctt
     7261 taagcaatgt agattttcca gcaccatttg gaccaataat acctgttaaa gcaccttctt
     7321 gtatttttaa agaaatctcg ttcaatgcta aaaaatcgtt ataacaaaca ttaagctttt
     7381 tcacttctaa cataactttc tccttctaaa tg
//
LOCUS       DAJSMI010000127         7372 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJSMI010000127 DAJSMI010000000
VERSION     DAJSMI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.128"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            166..1134
                     /locus_tag="ON430_002165"
     CDS             166..1134
                     /locus_tag="ON430_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3220070.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1145..1960
                     /locus_tag="ON430_002166"
     CDS             1145..1960
                     /locus_tag="ON430_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR3220071.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2028..2738
                     /locus_tag="ON430_002167"
     CDS             2028..2738
                     /locus_tag="ON430_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3220072.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            2774..4015
                     /locus_tag="ON430_002168"
     CDS             2774..4015
                     /locus_tag="ON430_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR3220073.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINYFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKNESSPQSCVKVQPHQKH
                     NVLRCEPNCHAFLSEERGQEQ"
     gene            complement(4073..5260)
                     /locus_tag="ON430_002169"
     CDS             complement(4073..5260)
                     /locus_tag="ON430_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCR3220074.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            5370..5747
                     /locus_tag="ON430_002170"
                     /pseudo
     CDS             5370..5747
                     /locus_tag="ON430_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            5795..7195
                     /gene="yfcC"
                     /locus_tag="ON430_002171"
     CDS             5795..7195
                     /gene="yfcC"
                     /locus_tag="ON430_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR3220075.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            7228..>7372
                     /locus_tag="ON430_002172"
     CDS             7228..>7372
                     /locus_tag="ON430_002172"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220076.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT         2283 a         1228 c         1549 g         2312 t
ORIGIN      
        1 tttttagaga aataaatttt tcctattgac aaacacaaag aaagcggata caatacaatt
       61 atgtcacatg acgttatatg acgttatgta atatctttac atttaatcag ttacttttat
      121 tttggataaa attaacattt ttaaaatttt aaggaggaaa tgttgatgga tgtagcaaaa
      181 acggttaacg caattgtaga aaaaaatgat ggagagattt ctggaattta tttggtggct
      241 tgtggcggtt cgctggtaga tatgtatgtc tcagattatt ttcttaaagc cgaagcatcg
      301 aagtgtatta caggcttata tacagcgaac gaatttaacc atgtaacccc aaagcaatta
      361 gatgataaat ctgttgtcat tctatgttct catggaggaa atacagcaga aacggtaaaa
      421 gcaggagaag tcgctcaagc tagaggagct gtgactgttg gtctgactca taataaaact
      481 gcggatttat taaaggtttc tgatcatagt ttcctttacg aatggggaga tgatgcaaaa
      541 atagtcaata atcctatggc catcattctt gatttaactg tatctttggt caatgttacc
      601 gaaacatttg aacattacgg taaattcaga gaaggcatga aggttatcga taccgtaatt
      661 gaaaatggca ttaagcaggt gcaggatcgt attaaagtgt ttgctgataa gtacaaagat
      721 gaaacaatgt tttatatctt aggtagtgga ccatcatatg gacatgctta tggtttttca
      781 atttgctctt tgatggaaat gcaatggctt gattcaactt ctgttcattc tggagaattc
      841 ttccatggac cttttgaagt aacagacaaa gatacgaatt ttattctctt gatgagcatt
      901 ggacgcactc gtgctttaga tgaacgggca aaacgattcc ttgataaata tgcagaaaga
      961 gtagaaatcg ttgatgcaaa ggaactagga ttggatatga tatcagatga agtttctgaa
     1021 ttcttcaatc ccattttatt ctacagtatt ctttcagaat atcgttcagc tttagcagat
     1081 gcacgcaatc atgatttaga tgttcgtcgc tatatgggga aagtggagta ctaggaggat
     1141 taaaatgaag gtattaggct taggtgataa cgtcgtcgat aaatatgaaa atctgggaat
     1201 tatgtatcct ggaggaaatt cattgaattt tgcagttttt gcaaaaaaac tgggacatga
     1261 aagtagcttt atgggagttt ttggctcaga tgaggaggca gctcatgttt tatcagcaat
     1321 aaaaaaaata ggactggaca acagtcactc tcgctttgaa aaaggcgaga atggctgtgc
     1381 cagagtgaag attgatgagg gcgaccggat tttccttggc agcaattcgg gaggtgttac
     1441 tcgtgaatac ccgattcagc ttactgaaga agacagagaa tatttaaacc agtttgaatt
     1501 gattcatatg ggattatata gccatgtcaa tcatctatta gaagagttac aaaatgtttc
     1561 tggaaagatt tcttatgatt tttctgacga ctttacggaa gaagaggttc agcgggcaat
     1621 tgataaagtt gattttggct tcttttctat agaagacttt tccgatgaag aagtaaagca
     1681 gtttttgatg aagcattttt gtgagcgaaa tgatgtattg attgcaaccc gaggagaaaa
     1741 aacggctatt ggttatgatg gagcaaagtt ttatgaagtg aaccccgtgc tagaagaacc
     1801 agttgataca atggcagcgg gagattcttt cttaacagct ttcctacttt attttctcga
     1861 aggggacgat atagtcaccg cgatgaaaaa ggggaatgaa tttgctggga agtcttgtct
     1921 ggttgacggt tcgttcggct ttggtattca ttacgattga gcttgtttta aattcctgca
     1981 tttgataacc taaagaaaga aatcccggta aagtggaggt taaaaaaatg ctggatacaa
     2041 atgcacaatt accattgtac ttacaattaa aagaaatatt aaaaaataaa atcaatacag
     2101 gggagtatcc cgagaatacg aaaattcccg ctgaatcaga gctgatagaa agttatgagg
     2161 tcagccgaat cacagtgaga aaagcagtag aagaactggt agaagaaggg tacctactta
     2221 aaaaacaagg gaaaggaact tttgtcaacc ggcataaagt ttttcgtaaa attgaatatg
     2281 ttatgggttt cagtgagagt tgtaaatcta atggttttac accgactagc attgttttgg
     2341 aacgcaagat cattccagca actgaaggga tggcaaaaaa actcgatatc gctcaaggtg
     2401 atgatgtttt atatatccag agaaagcgaa tggcggataa catccccatt atgttagaaa
     2461 acaattattt ttcttatgaa agatatagtt tcttaatgga ggaagagctg actgattctt
     2521 tgtatgaagt acttaaagtc caaggtacct tagccatcaa tcctggggaa actgcacttg
     2581 aaattgtcac agctgatgac caaattgcca aaataatgga agtagctgtt gggacgccct
     2641 ttttctttat ggatacttta atcaacgatc aaaacaacat accaattcat gtaggtaatc
     2701 aatattacct gggagaagta tatcgttttt acttatgatt tcctgttttg atttcaagga
     2761 gtagatttta tttatgaagc aaatattgcg gtcaaaaatg atttttgatg gagagcagcc
     2821 acctttcaaa ggttatattg taatcaatga agggatcatt gaaaaggtgg aaaacaactg
     2881 gaattacagt gatctattaa atgaaaacac ggaattaatt gcttatgatg atcaattcgt
     2941 tatgccagca attcatgata atcatgtatt tttcagtggt tatttggcta tgaatgctgg
     3001 catagattta agcgatacat cttcagctaa cgaggctgta gagcgtatta aagctgtaat
     3061 gaaccataaa aaagcagatg aaccaattta tgctcatggc tgggatgctg ccctgtggaa
     3121 aactgaccca tcagaagagc tattgaatga aataaactac tttggtccaa tcacagcgat
     3181 cgacaaaaat cgcagccatt gttggatgaa tgctattgct aagcagactt atggttttac
     3241 agaaaaagaa accagtgctg aaagtcgagt attattaatt aaggaaatgc tagcaaacaa
     3301 aaagctttta gcagaggttt acgctgaatt tgaagcatta ctgctttcaa aaggtgttat
     3361 atcaatgaaa gaaatcgttt ttgatgacgc agaatttctt tctaatatac ctcagaggaa
     3421 aatacttagt aacctatatg tgcaggcagt ccaagaacct ctacagcatg aacttctata
     3481 tgagtataag gaaaaagttt tccctgaaaa ggtccgcttt ggtggagtga agatcatggt
     3541 agatggtgtt gttgcggata gaagtggaga tatttacggt gtttatcagt caggagtagc
     3601 ttcacccgag attgactatg aggcaattaa agcagaagtt gctgcattca atgaagtaga
     3661 gattccttgc tgtttgacaa cggaaggaga taaagcgggt tataaagctg cgtcaattct
     3721 tgtcgaattt ggaaagcggt tacctgaagg cgtttttaat tcgattagtg atttggaaat
     3781 ggttactcaa aagacagcag aaaaaatgaa cgaaggacaa gtagtagcag aggtatatcc
     3841 acaaattctc ggcttgaatc aaacgttaga agaggcttac atgtcaactg ttataaaaaa
     3901 tgagtcaagt ccacaatctt gtgtaaaagt tcaacctcat caaaagcaca atgttttgcg
     3961 ttgtgaacct aactgccatg cctttctttc tgaggagaga ggtcaggagc agtagcgcag
     4021 cgactcttga tgtctctcct cagaaagaag agactcgcac aggttcatgg tgctaaatgt
     4081 tattcgaatt aaattctaaa tattttcgtg gagaagccag ccactcagtg tctttttcca
     4141 tcaaaagtgt tccaattatc cgagtgatgg aattgatatt tggaaaaatc cgaatcactc
     4201 gttctctccg gcgaatctct tcgttcaacc gttccaacac attggttgta cgaatccttc
     4261 ggcggatatt ttcaggaaaa gacaaaattt gaatagcatc ttcaaagccg ttttctaaaa
     4321 tgtcgcaagc agcacttaat tttgaatcac aaccatattt ttccaagaag tgctcttttc
     4381 tttcacgtgt caattcaagt tcagaggctt taaagatact cttcaactcg tcttttacat
     4441 cagaagaaac tttctttggc agcttatcaa aaatatttct caaaaagtgg gcttggcatc
     4501 tttgccagct cgcacctaaa aagttcttgc gaacagcctt aactaaccct ttgtgtgcat
     4561 cagaaatgac cagttttaca ccgaacaaac ctcgttgaac gaacgattga aaaacggtat
     4621 cccaattatc ttccgattcg ccgtcttgaa catcaaaacc aagaatcgta cgtcgtccat
     4681 cagaatcaat gccgatacca atatatacac cttttgagac aatacgatga ttttccctta
     4741 ctttcatata cagggcatca cacatgaaaa atggatattc cgagccttct aggctccggt
     4801 ttctccactc aaaaacaaat ggatccaact gtttcatcag atttgaaacg aatgatttag
     4861 aaacagtcgc accatccgtt agcagttcaa ctgttttagt tactttgcga gtggagacac
     4921 cagaaatgac catttcaatc atggcagcga tcaaagcttt ttcgcttcgc tgatatcttt
     4981 caaagatagt aggactaaac ttaccgtctc tggttcgtgg aacatgaagt tctaacgtac
     5041 caattcgagt tgtaaacgaa cgatcgtagt aaccatttct ttgtgattgc cgttcttctg
     5101 aacgttcata ggcatttgcc tgaatatatt gatttcgttg ttcttccatc agctgattaa
     5161 acatgatagt cataagtgtt tttgcggtat cattcgcacc gctttcgttt ataattgctt
     5221 gaatttcttc gctttcaaaa gtaaaatgta cttgagtcat tgatatcagt cctattcgag
     5281 tatgttttct gcaaaaaaca ttgtactcct aactgagatc aatggctttt ttgatgcttc
     5341 acttttacac aattatatgg acgtaatcaa aaaatgtttc cacagaggac ttctttaatt
     5401 attgtcgatt agaggaaagt ggtgtaacag tcactagcgg tacagattta ccgctgttta
     5461 ttactgatat tcctgaatcg attataagag cagtattccg tcgttttcct gaaggagaag
     5521 aagtctggga gaaagaaaca agcttcacga caaccgagct gctgaaaagt tttacaagca
     5581 acagcttcaa agtaaatggt ttatcggagt acggagaaat aaaagtaggg aaaagcgcca
     5641 gcttagctgt tttcagcgag gacctctgta ctgatgactg gcaaaaagtg atggctgcgg
     5701 aagttatcgc tacctatctt gatggtgact tagtttacaa gaaataatgc ttttcgatat
     5761 aacgttataa aacgaattaa agagaggtta aaaaatggaa acaatgaaaa gaaaattcac
     5821 cttcccacat agttatacat tgatttttat cttaattatt ttggctgcaa ttttaacttg
     5881 gataattcct agtggtcagt ttgagacagt tgttgaaaac gtcgcaggta tggaaaaaac
     5941 aacggttgtt ccgggaacct atcaccaaat cgataaggca gattatggac aaggtttttc
     6001 ctctatacta atggcaccag ctgaagggat tatttcagct gttgaggttg ttgcttttgt
     6061 tctcatcgtt ggtggtgcct tcgggattat cctaaaaacg ggagccatcg atcgagggct
     6121 gtttacctta gcaaaagttc tgggagacaa ggggatctta gttattccga tgtcgatgat
     6181 tctattcagc ttaggaggat caacctttgg catgagcgag gaagtcattc ccttatttgc
     6241 ggtgtttatt tctctgatgt tttctcttgg ctatgattcc atgactgcga tcttgattct
     6301 atttttaagt acacaagcgg gatatgttgg gtcgacgatt aatcccttca acgttttaat
     6361 cgcacaggga gtagctggcg tttacggaaa tcccctgctt tggtttcgga tgctatcctg
     6421 gattgtcatt actgggattt caattgtttt caccatgctt tatgctagca gagtcaaaaa
     6481 gaatccactg tcctctattg tttatgagag tgatttaaag gttaagcata agtttcaaaa
     6541 ttttgacact gaaaaagagt tttcaacaca agataaacta attatcgccg gttttatcgt
     6601 aggtttagga atcatgattt ggggtatcat ttccaaggga tggtatatgg ttgaaatttc
     6661 tgcactattc ttaggattag gtctttttgc tgggggcatc gctagatttt ctcaaaagga
     6721 aattgctgaa gcttttgtcg aaggctgttc agatttcgcc tatgctgctg taatcattgg
     6781 gctagctaga ggagtcttgg taatcttgga aggtggaatg attattgaca ctattctaaa
     6841 cagcttggct aaccttttag gtggtcttcc aaaattcctg ttcactacga ttctactcat
     6901 ttctcaagta attgtttcct tttttgttcc atcctcctca ggagctgcgg cattaacgat
     6961 gccggttatg gcaccattgg gtgatctaat gaatgtaagt cgtgatgttg ttgttctaag
     7021 taaccagttt ggaaatggtt taatgaatat tatctcccca actagtggtg tattactggc
     7081 aggattagcc atcgcagaga ttaatttctc taaatggttt aaggttggct tcaaaatatt
     7141 tgccatctta acccttgttt ctgctgtttt gttatacatt gctacactct tttagtctta
     7201 atcatccatt aataggaggc aatagttatg attaaaataa atcaattagc aggaatgaat
     7261 atccactatt tattttattc tctcaaagaa tttttcgctt atcaacagaa aattggtatt
     7321 tcttctgttg aactttgggg aggtgctccg cattttttca tagatgcttt ta
//
LOCUS       DAJSMI010000128         7364 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJSMI010000128 DAJSMI010000000
VERSION     DAJSMI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.129"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            46..3738
                     /gene="nifJ"
                     /locus_tag="ON430_002173"
     CDS             46..3738
                     /gene="nifJ"
                     /locus_tag="ON430_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR3220077.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            3927..4814
                     /locus_tag="ON430_002174"
     CDS             3927..4814
                     /locus_tag="ON430_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCR3220078.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            4811..5980
                     /locus_tag="ON430_002175"
     CDS             4811..5980
                     /locus_tag="ON430_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220079.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6004..7359
                     /locus_tag="ON430_002176"
     CDS             6004..7359
                     /locus_tag="ON430_002176"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR3220080.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2397 a         1360 c         1715 g         1892 t
ORIGIN      
        1 aatggaacag ataataagag agaatatgaa caaggatgat gaatcatgcg aaaaatgaaa
       61 acaatggatg gcaatacggc agccgcatat atttcttatg catttaccga gattgctgca
      121 atatatccaa taacacccag ttcgaccatg gctgaattag tagatcaatg gtcagcagca
      181 ggaaaagaaa acgtattcgg tcaaccagta aaagtagtag aaatgcaatc cgaagcagga
      241 gcagctggtg tggtccatgg atcattgaaa actggtgcac tgacaactac atataccgct
      301 tcacaagggc tgcttttgat gataccgaat atgtacaaga ttgctgggga actactgcca
      361 agtgttttcc acgttgctag tcgggcagtg acgacaaatg cgttgaatat tttcggagat
      421 cacggagacg tgatggctgc aagacaaaca ggatttgcta tgttagctga aagcagtgta
      481 caagaagtga tggatttagc acctgttgcc catcttgctt ctattgaagg agaagtccct
      541 tttttgaatt tctttgatgg attccgtacg agtcacgaga ttcaaaaaat cgaagttttg
      601 gaatatgaag aattagctcc tctgatcaat caggagaaat tagcttcatt tcgtaaacga
      661 gggatgaatc ccaatcatcc gactgttagt ggtacgaacc agaatccaga catccatttt
      721 caacaacggg aaacgatcaa cccctattat gataaattac ctgaaatcgt tcaaaaatat
      781 atgaaagaga tcaaccgatt acgaggaact tcttatgact tggtgactta ttacggagca
      841 gacgatgcgg aagaagtcat tgtttctatg ggatcagttg cacagacgat tgaacaaacg
      901 attgattact tgaatgcaaa cggacggaaa acaggttttt tgaatatcca tctttatcgt
      961 ccttttccag tggaaacatt cgtgcaaaag ttgcctcata cagtaaaaac aattgctgtt
     1021 cttgatcgaa gtaaagaacc aggggcaggt ggagaacctc tcttattaga cgttcaaagt
     1081 gcgttatatg attctgaact tcgcccggct gttattggtg gtcgctacgg attggggtct
     1141 aaagatgtga ctcccgacca aatctcagca gtttttgatg aattgaaaaa agacccaagc
     1201 atacgcaaaa aacggtttac catagggatc gtggatgatg tgacttatca atctttggaa
     1261 ccaaaggaaa gtctggattt gacagaaccg caaacattcc aggccaagtt ttggggattt
     1321 ggatctgacg gaacagttgg agcaaataaa tcagctatca agattattgg agatcacacc
     1381 gataaatatg cgcaaggcta tttttattat gattcgaaaa aatctggcgg tctgactgtc
     1441 tctcatcttc gttttggaga tacgccgata cgttcagcgt atcttgtcga gcatgcggac
     1501 cttgttgcct gtcacacacc agcttacctg catagctatg acttggtcaa aggattgaag
     1561 ccaggtggca tttttttatt gaatactctt tggagcgacg aacaattaga aacgcatctg
     1621 cccttaaaat taaaacgtta cttggctgaa aataacatta gattctatac gatcaatgcc
     1681 atgagattgg cccaagaagt cggtttggga cgccggatca atacggcaat ggaaacggct
     1741 ttttttaaat tagctgacat cattcctttt gatgaggtgc ttccgctgct aaaagaagaa
     1801 gccctgaaaa gctatggaca taaatcaatg gctatcgtag aaaaaaacat acaagcaatc
     1861 gatcggacga ccgaactgct tcatcaagtc gcgatcccag accattggag aacgttggag
     1921 atgcctgaaa aaatcaataa aaggaacact ggttatatac aagaaatact tgaaccagtg
     1981 aatgcacagg aaggaaatca actctctgtt ggtacacttg tagcaaatgg aatgacaact
     2041 ggcgaaatgc ctttaggaac ggcttctatg gaaaagcggg gcgtcgcttt agaagtacct
     2101 gagtggatta gtgatcgctg tacgatgtgt aatgaatgtg cctttgtttg tcctcatgct
     2161 gccatccgtc catttttagc agatgaagag gaaatggaag aagcgccaga aggatttatc
     2221 gtgagagaaa tgcgaggcgc agacggcgta aaataccgta tccaagtttc agtggaagac
     2281 tgtacaggtt gtggtctgtg tgtagaggct tgtcccgcaa aagaaaaagc tttagtgatg
     2341 aagccttatg aagaacagaa agaacaagct gtgaattggg cttttgcaat gacattaaga
     2401 caaaaagcaa atccagtcaa aggaaagaat actgtcttgt ctacacaatt caatcaaccg
     2461 cttcttgaat tctcgggcgc ttgctcaggc tgtggcgaaa caccttatgt aaaattattg
     2521 acacaaatgt tcggtgatcg aatgatgatc gcgaatgcga caggctgttc ttctatttgg
     2581 ggcggtgctt ctcctgcttc tccttatacg accaatgaat gtggtcaagg gcctgcatgg
     2641 tcaaattctt tattggaaga taatgctgaa tttggttatg gtatgcttct agcggcacag
     2701 actcgtcgcg aggctttggc aagttcaatc aaagaagtat tgccaaaagt atcagaagaa
     2761 ctacgagaat tgatggaaga ctggttagta cacatgtttg agagtgaagg aacgcagcaa
     2821 agagcagcaa aactgcaagc tgcgatcgaa agcgaacgat caattttccc tgaactggag
     2881 gatatataca agagaaagga tctattcgta aaaaacagcc aatggatgat tggtggagac
     2941 ggatgggctt acgacattgg ttttggcggg atcgatcatg tacttgccag cggagcagac
     3001 gtgaatatcc ttgttttgga taacgaagtc tattccaata ctggcggtca aacctcaaaa
     3061 gcaacaccag cttcggcaat cgctaaattt tcggctagtg gaaagtatgc ttcaaagaaa
     3121 gatctaggaa tgatggcgat gacatatgga aatgtttatg tggcgcaaat tgcttcaggt
     3181 gccaatcaaa tgcaaacgat caaggcattt gaagaagcag aaaagtatcc tggtccttct
     3241 ctgattattg cctatacgcc ttgtatcaca catggcttgc ttggcggaat gaaagaatcg
     3301 cttaaagaag caaaagaagc tgtttcttct ggttattggt cattatatcg atataaccct
     3361 acaatggtcg aaaaaaacaa gaacccaatg actttggact acaaaaaacc tgatttctct
     3421 gctatgcctg atttcatgag gaaacaagtt cgcttttcct cgctagataa cgcaaatcca
     3481 aatcttgcaa aagctttatt tgaaaagact gtccacgacg ctaaaacacg tttttacaac
     3541 tatgctgcac tgacgggaga attagaaaaa atcaagaaac gacttgaacc agaggaatct
     3601 atcgatgtac ccgttaagac tagaaaagaa aaaacgacgg accttgaagc agaagaaaga
     3661 agagctgcta gaagagctgc cagagcagaa cgacgtaaaa aaatggaaca agctacaaaa
     3721 gaaaaaaagg aaaaataaca gaaaaaatat aatgaataat tctctgaagt ttaaaaatat
     3781 ttattgcaga gaaaaataaa acgcatactt gcaattttct tgaaaattat gcgtttttct
     3841 caagaaaaat acgagcatat cagcctctaa aattaaaaca tatgatatac tagagaggaa
     3901 atctcaaaag agaagagagg ctttttatgt catttcaaat cggggaactt tttaatttgg
     3961 actattggca gcaaatgttt tccggtgact tgttctctct gaattttttg gtgaacatac
     4021 ttgatattct ggttgtctgg tatcttgtat acaaactgat ccaactgctg cgaggcacaa
     4081 aagcgattca gcttctgaaa ggtgtagcta tttttattgt tatccgaatc gtagcggaaa
     4141 tcattggtct gcacaccttg tcttggctga tgaaccaagt cattacgtac ggggtgatag
     4201 ctgctatcgt gatttttcag ccggaaatcc ggagaggact ggaacatctt ggacgcagtt
     4261 cgcttttcaa acaatcaaaa agcgaacagc aggaagatga gaagatgatc ttgtcttttg
     4321 acaaagccat ccaatatatg tccaagcgaa aaatcggtgc gttgatcact atcgaacgtc
     4381 atactggatt agatgagtat attgagacag ggattgcttt agatgcagat atcactgggg
     4441 agctgctgat caatatcttt attcctaata caccgctgca tgacggtgca gttattgtaa
     4501 aagaaggaaa gatcgcagta gcaagcgcct atcttcctct atcagaaagt atgttgatcc
     4561 ctaaagaatt cgggactcga caccgggcag ctgtcggtat aagcgaagtc agtgacgcga
     4621 ttaccatcgt tgtctctgaa gaaaccgggg acgtcagcat cacgttagat aatgaactga
     4681 tggctggctt atcccagcaa gaatatttgg caatcttgcg tagagaacta attgctgaag
     4741 agaaaacaaa aaacaagaaa aacgtgattc aatctttcct agacggtatg actaagggag
     4801 gggatcgcaa atgatctcaa aagagaaacg gacaagcatt ctttacagtt ttattgcatt
     4861 gctgttcagt attgcgctgt acttcaatgc aaatggccag tccgttccaa atacgctttc
     4921 tggaaatgaa gcgtataatc aaacagttac gggggtgccg atcaagattt cttatgattc
     4981 aaagcgctac tatatccatg gatatgaaaa tgcagtgact gtcaaacttt ccagtgcaaa
     5041 ccgagttcag ttgaatgcag aggcaaacga agatacacgg atgttccgcg tcacagcaga
     5101 tatcactaag ctgggcgaag gaacacatga agttccgcta aaaattcaaa atttgagcag
     5161 tgctgtcaca gcaaagatcg aaccatcgac tattacagta acggttgaga aaaaggtaat
     5221 aaaagatttc gatgtggaaa cgttgattcc ttcaacgttc acaccatctg gatatgaact
     5281 tgatgatact tccgttagtc ctaaaaaagt ctctatcact acaggtgaca aaacattggc
     5341 agaaatcaaa cgagtagtag ctgatgtgga cgcttcgatg gtcacagatg atgggattaa
     5401 tagtgaggtc ccaatacagg cgctgaatgc agcaggggaa gtattaagta tcgtctctga
     5461 tccagtgcaa gtaacagtga gagctgattc gatcaagcca agcaagtcgg tacgacttta
     5521 tgggatacag caaggaacac cagctgccgg ggtagaaagc tatgatttca gtttttcaaa
     5581 actggaagca gaagtaagcg gctctagcga tttgcttgca tcgatcggtg acagtattgc
     5641 tgtaccgatc aatgtctcag gtatcaatca tcgaacaact cgtaaaattg aaataccagt
     5701 agaagagggg ttaagtatca agcccgaatc agttagtgtg gagattacac ccgtgttgca
     5761 aacgtcaagc agtagctctg cttcgacgag cagtcggaca caagaaaata caagtacgag
     5821 tagaagtatt tcatctgctg attcaggaac ggaaccatcg acaacaacca tttcttccag
     5881 ttcggaatct gggacaagca gctcgaccac aagtacagaa gaaacatcag aaacaagcga
     5941 atcaacacaa atgagcagaa caaattcctc agaaaattaa gttaaaggag taattttaca
     6001 actatgggta aatattttgg aacagacggc gtacgcggtg aagccaacaa ggaacttacg
     6061 ccagaacttg catttaagct gggacggtgc ggaggttatg tcctaagcca gcatgaagaa
     6121 gaaggaaaac gcccacgcgt gttagtaggg cgggatacaa gaatctcagg tcaaatgtta
     6181 gaagaagcgc tgatttcagg acttttatcg gtgggtatcg aagtgttcca gctaggtgtg
     6241 atctctacac caggtgttgc ttatctgaca cgtttgcaaa aagcaagcgc aggggtcatg
     6301 atttctgctt ctcataatcc agcacaagat aatggaatca aatttttcgg tgcagatggt
     6361 tttaaactag tagatgacca agagctagaa attgaagcat tattagatgc tgaagtggat
     6421 gaattgcctc gtccatcagc tgaaggattg ggaactgtag aagaatttcc agaaggctta
     6481 ttgaaatatt ctcaattcct tgtacaaacg atcaacggtg acctttccgg tttgaccatt
     6541 tgtgtcgatg cagcaaacgg agcaacttca acaagtgtta atcgtttgtt tgccgatctt
     6601 gaaacagatt tttatacaat gggcacaaat ccagacgggt taaatatcaa tgacggcgta
     6661 ggctctaccc atccagaacg tttagctgag atggtcgttg aaaaaggagc agatgctgga
     6721 ctagctttcg atggcgatgg cgatcgtatt attgcggtgg atgaacttgg aaacattgtc
     6781 gacggtgata aaatcatgtt tatctgtgct aaatacttgg cagaaaaaaa tcgattgaaa
     6841 aaagatacga tcgttacgac tgtcatgagt aacttaggat tccacaaagc ggtggaagga
     6901 atcggtttga aagatgtcgt tacacaagtt ggtgatcgtt acgttgtaga agaaatgcgc
     6961 aaaaacgatt ataactttgg cggcgagcag tctgggcaca tgattttctt agattttaac
     7021 acaactggag atgggatgct ttcaggaatt cagttactga atatcatgaa acagacaggt
     7081 aagaaattat cagagcttgc agcagaggta acaatctatc cgcaaaaatt agtgaatatc
     7141 cgcgtaagtg ataaaaacgg tgcgatggat gttccggcaa tcaaaaaagt gattgaagag
     7201 gcagaagctg aaatgggatc agaaggaaga atcctagttc gtccttccgg tacagaacca
     7261 ttacttcgag tgatggcaga agcgcctact gatgagaaag ttaattacta tgtcgataaa
     7321 atcgcggcag tagtcaaaga agaaatcgga ctggattaag agat
//
LOCUS       DAJSMI010000129         7356 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJSMI010000129 DAJSMI010000000
VERSION     DAJSMI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.130"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ON430_002177"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ON430_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR3220081.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(1121..2497)
                     /gene="trmFO"
                     /locus_tag="ON430_002178"
     CDS             complement(1121..2497)
                     /gene="trmFO"
                     /locus_tag="ON430_002178"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCR3220082.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(2466..4544)
                     /gene="topA"
                     /locus_tag="ON430_002179"
     CDS             complement(2466..4544)
                     /gene="topA"
                     /locus_tag="ON430_002179"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR3220083.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(4666..5523)
                     /gene="dprA"
                     /locus_tag="ON430_002180"
     CDS             complement(4666..5523)
                     /gene="dprA"
                     /locus_tag="ON430_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR3220084.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(5579..6346)
                     /locus_tag="ON430_002181"
     CDS             complement(5579..6346)
                     /locus_tag="ON430_002181"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR3220085.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(6339..7199)
                     /gene="ylqF"
                     /locus_tag="ON430_002182"
     CDS             complement(6339..7199)
                     /gene="ylqF"
                     /locus_tag="ON430_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCR3220086.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         1900 a         1629 c         1289 g         2538 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agagcgagac tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag
     1141 ttttttctaa ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt
     1201 catatcttgc ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg
     1261 cattcatcgg ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa
     1321 tagctgtttc ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga
     1381 ttccagcaag tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac
     1441 ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg
     1501 aattcataaa gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc
     1561 caggaatcat ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca
     1621 cgatgttgta taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac
     1681 gtttgccagt tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt
     1741 tgaatcctcg ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct
     1801 caaaagattt aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt
     1861 cctcttctgt catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca
     1921 aatatacttt atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga
     1981 aataaaaacc atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca
     2041 aaggtcctgt tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca
     2101 ctgttactaa tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt
     2161 caacagctaa ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg
     2221 aattcaatcg gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc
     2281 ttaatgaatt ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt
     2341 tgactggacg catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag
     2401 cttcacttcc ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt
     2461 ttctattatt tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg
     2521 cctttgactt tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt
     2581 ttatcccaag aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt
     2641 ttcttagatt ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt
     2701 tcttttacga ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc
     2761 ccatatttcc ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct
     2821 ggttcatctt tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca
     2881 aacggtttat agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta
     2941 tccaaatctc cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaattca
     3001 cagattaatg aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat
     3061 tttacatagt agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct
     3121 accccgtttt cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga
     3181 gtaaaatgtt gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct
     3241 ggaagaatat tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg
     3301 aatcctttga atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat
     3361 gtcactttca ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag
     3421 atcaatgtat aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta
     3481 cgaagcacac tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg
     3541 atctttcggt gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg
     3601 gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag
     3661 ccgactgttc cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg
     3721 gtttttcttg ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg
     3781 aaaggattcg cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct
     3841 gttccatcaa ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc
     3901 ttaccgtcga ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg
     3961 tcgatgctcc aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata
     4021 atttttaaag cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag
     4081 agaataggac tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca
     4141 tctactaaat ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt
     4201 gtgatttcat tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct
     4261 aaatgccaag caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga
     4321 gctttttttg ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata
     4381 taatggggtt cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt
     4441 atatggccaa cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg
     4501 gcttttgcag gtgattctac aatcactaaa tatttatacg ccattactga atggactcct
     4561 ttccattgtc gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc
     4621 cattcagaaa acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc
     4681 tgccaagata tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca
     4741 tccggtagaa tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc
     4801 caatgcttgc tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt
     4861 tcctttagac aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc
     4921 ggattgagga ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc
     4981 acgattttca gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc
     5041 accatgagag attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac
     5101 gatcacatat ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa
     5161 agcagaagtg tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc
     5221 accattataa aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt
     5281 gggatcaaag caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat
     5341 ttcctgccaa cttttgcgaa acagctcccg gtgtttggtt attttaccta tcttaatgat
     5401 gtcttcaaat ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt
     5461 tccttgacta ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata
     5521 catcataaaa ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt
     5581 attgtaaata tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc
     5641 gttcaagtcc ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc
     5701 caggataaag cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac
     5761 ttgcagcagc aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag
     5821 gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta
     5881 ctgcttggat catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct
     5941 gatcaacgat cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt
     6001 ctcgcttttt ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg
     6061 gcaagataac tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa
     6121 tgccggcaat ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt
     6181 catatttttc cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt
     6241 gctgcacgcc tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct
     6301 gtatgctttt caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc
     6361 atttcttcgc aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg
     6421 actaattcgc tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc
     6481 agtaattccg gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt
     6541 cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg
     6601 acaagctggt ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca
     6661 aatttgggcc ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc
     6721 cactgctgac cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact
     6781 agtcgattca tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata
     6841 gcgcgaggct tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta
     6901 gccgcaggta ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga
     6961 tggccttgtt tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc
     7021 gctttattca ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta
     7081 cgagaagata aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt
     7141 ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt
     7201 gttctctcca ttcctttcat accggaatat tccccggctt tacttgtttt tttctttttc
     7261 aatataaatt cactttttta ttataaagaa attccatcta ttagttaagt gaaagtgaac
     7321 cagtcctcat tctatcacat cttcactttt ttgatg
//
LOCUS       DAJSMI010000130         7301 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJSMI010000130 DAJSMI010000000
VERSION     DAJSMI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.131"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(218..475)
                     /locus_tag="ON430_002183"
     CDS             complement(218..475)
                     /locus_tag="ON430_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR3220087.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(468..737)
                     /locus_tag="ON430_002184"
     CDS             complement(468..737)
                     /locus_tag="ON430_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3220088.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(868..1047)
                     /locus_tag="ON430_002185"
     CDS             complement(868..1047)
                     /locus_tag="ON430_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220089.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(1428..2135)
                     /locus_tag="ON430_002186"
     CDS             complement(1428..2135)
                     /locus_tag="ON430_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220090.1"
                     /translation="MNKMIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSHNRLDMYKRRPDTLKKGAELLRLETEKHIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMQYQRFERKKGQ"
     gene            complement(2128..2565)
                     /locus_tag="ON430_002187"
     CDS             complement(2128..2565)
                     /locus_tag="ON430_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220091.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            complement(2581..2832)
                     /locus_tag="ON430_002188"
     CDS             complement(2581..2832)
                     /locus_tag="ON430_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220092.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            complement(2832..3038)
                     /locus_tag="ON430_002189"
     CDS             complement(2832..3038)
                     /locus_tag="ON430_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR3220093.1"
                     /translation="MRKKYDYWGVPFSELFPNYHAPHTIECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            complement(3479..3739)
                     /locus_tag="ON430_002190"
     CDS             complement(3479..3739)
                     /locus_tag="ON430_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220094.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3814..4017
                     /locus_tag="ON430_002191"
     CDS             3814..4017
                     /locus_tag="ON430_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCR3220095.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(4158..4643)
                     /gene="ssb"
                     /locus_tag="ON430_002192"
     CDS             complement(4158..4643)
                     /gene="ssb"
                     /locus_tag="ON430_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3220096.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFVNCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKATTESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(4646..5545)
                     /locus_tag="ON430_002193"
     CDS             complement(4646..5545)
                     /locus_tag="ON430_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR3220097.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFADTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(5558..6151)
                     /locus_tag="ON430_002194"
     CDS             complement(5558..6151)
                     /locus_tag="ON430_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR3220098.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(6226..6375)
                     /locus_tag="ON430_002195"
     CDS             complement(6226..6375)
                     /locus_tag="ON430_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220099.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(6391..>7301)
                     /locus_tag="ON430_002196"
     CDS             complement(6391..>7301)
                     /locus_tag="ON430_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HCR3220100.1"
                     /translation="KAIGKVPLDLGWKKLFGKESDGKEKEEEPLLPKVTKGEMVTVDL
                     QVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLK
                     QKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGERKGTQTTFL
                     ANIQKFVSHLLEVVPGQIQSTNFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYQ
                     KVAACTSEACDFKLWTTIAKKKLTATQLKEIIQNSRTSQPVKGLKGQKGSFEATIVLK
                     EDFTTGFEFSEKKKTNFKKRTRRTAK"
BASE COUNT         1922 a         1475 c         1181 g         2723 t
ORIGIN      
        1 ctattcaact tcaaattgtt gaaatttaag ccatttatat ttacttttcc tcaactttag
       61 aaatgatttt actaacctta tatccgtaat acgcgtaaca aacacattca ttaataacaa
      121 tcaacactaa gctatattag ctttttaaat gactagggta aataaaataa ggatcaaaaa
      181 cttttaagtt tctgaccctt tccttacttc cgattggtta atagtgatct tttgcagaat
      241 aaataaatat cgtttcattt tcaactctat atatcagtct atgttcatct gtaattcttc
      301 tggaccattt tccagataaa tcatgcttta atggctcagg ttttcctaat ccagcaaagg
      361 gggaacgatc gatatctttt attaacttgt taatcttttt tatattgctt ttgtttcctt
      421 gctcatgcca ataaagataa tcatcccaag catcatcaga ccaagcctta atcatcagat
      481 tcaacctcga ttaagtcatg tgttttaaat gcacctttgg agaattgttc atctcctcga
      541 cgaatttttt ccatgacgta attattagaa agtgttctca acgtttcttg catagaatca
      601 taatctcttt ttgataatac aacaactgta tcttctacat ctttacttgt tacaataagt
      661 gtttcagcat cctcattaac ttgtttcata taactacgta aattttggcg gaaatttgaa
      721 taagctactg cttccattcc tttcacccct tatttatttc tataaaacaa ttgtacatta
      781 tattgtacaa ttaagcaaat gattaatatt ctaacttctt ataattaaaa gtgctatcac
      841 ttttagtttt taggataaaa agtgacgtca ctttttgagt gctggtacct caaatcctgc
      901 tttttcttgg gcaatcaata aatctaacgc aatacacata atcctggatt cacttttgcc
      961 gtacttctgc tttagttgct gcaattcttt ttgtctcttt tcatccaacg tcacgggtaa
     1021 acgtttattt cctattgcaa ttggcattag tactctctcc tttcatcgaa ataaccctat
     1081 taaaaatatg gctgcaatca acttgaatat tagactcaca agggttaaca caaagcgcca
     1141 aaagatcaaa caaaatacca gtattcctaa gaatactacg aagtttaaat gtagagctgc
     1201 accatgacgc ataagtatct cgccaacaaa cagtacgaac aatagaggaa tgattttatt
     1261 catatggatc tccttctttc tcttaggtta tcgggctttg cccgcttatt tcgcaccacc
     1321 taagcggcgg gggcggttgg agcgattggt gcgaaataag aaccattccc ttaatttcaa
     1381 ggttttttgt tcttatttca cacatcctca cttttcggta taaatggtta ttgacctttt
     1441 ttcctttcaa atcgttgata ttgcattcgt ttacggtcaa gttcattttg gatttcttct
     1501 gtatcgtcgt aagtgtaggt gtctaaaaaa tcagttaaag tcgtgtacca gtcgtcgcct
     1561 gggttatcta cttcttctac atattccctc aaaaatgctt cgggtacagc gagttcataa
     1621 tccattccca ttaacacgat agattccgtc ccacacgtca aaaatctagt aaatacaagg
     1681 taataatcgt ctaccgtcgc atctttgctg tttagctctt ccatttcttc ttccattaat
     1741 tccatgctct caaaaattga ataatattct gattctgagt aatagtgttt cctgcaaatc
     1801 gtgtagctga caaagttttc gaccagttca ctcctttcta ggtaaacatt catgatatgt
     1861 ttttctgttt ctagtcgtag aagctccgct ccttttttta atgtgtcagg acgacgttta
     1921 tacatgtcta aacgattgtg gctgactgta accttcgttt gactcgtttc actttccaaa
     1981 tacaattgtt cttccactct ttgaataaag tacttcattt cttccataca actaaagtct
     2041 ttttgaatcc gtctgattac ttcattcaaa tgttctttgt ttgttaaaaa gtgtgacaca
     2101 tcttccgttg ttagttgata aatcatctta ttcattttcg ttgtcctcct ttttcatggt
     2161 gtaatatgcg ccatacttat tgatattttc aaaataatct aaccattcac ttgaaacagt
     2221 cttagctttt ttcgtttcga taaatttcgc atacgttagt tctaaagctt ggtattcaga
     2281 aggtttgatt gtgcttggtt tgacgcctac ttccaagcat agtaaggctt ggaaaaacca
     2341 ttttgcattt tgctttttca gttcttccag tgttcgatag tacgtcgcaa tgagattcat
     2401 ttcgacacct gattcaatca tttcttccac agctaccgac aatctttcct tataatgttt
     2461 ttttggaaca cgtgtctcat tgtataggaa tgtcatatcc tccatttttt gtgccatttt
     2521 atccaaaggt aatttaacaa attcgtcata gtaattttga gccatatttt tttctccttt
     2581 ttagttctct aattcggtta accaatattc gttttcataa gtttccaagt tttcttgata
     2641 atgcagttca tatcctgcaa tcttcactag gtcttcaaat agcatagtcg gtgcatcctc
     2701 tactaaaggg gtttctatcg tccagtaccg cggaataagt aaagcttctg ctactttttc
     2761 agaaatgact tctttgtcca ccaaaatttg aacgacttct tttacagtca ataatttgtg
     2821 ttgtgttttc atcaacgttt ctttcctttc tcatcaatca aaatataatc gccataagac
     2881 aaggtatatt ttttcccgca tgttggacat tgatacaaac gatagctttt gatacatttc
     2941 gctctttctc cacaatcgca ttcgatagta tgtggtgcat gatagtttgg gaataattcg
     3001 ctgaagggaa caccccaata atcatatttc tttcgcatga ttgattctgc ctcctcttag
     3061 gaaaggtttt atttcctttc gttagttctt tctttattga tttgttggcc agaatgaatc
     3121 aacgaaaagc gacaaggaca agggcgcgca gcgtgccatt tacccttgtc cttgtcgctt
     3181 tttggtgata aaaggcaact tgttgccggt ctggcaaatc aataaagaga taacgaacag
     3241 gaacgaacct tttggtgaga ggaagttttg tctattttgt cttgctttgc aagacaccat
     3301 atcttagtga acgtgcagcg ttcacttcct ttcttctcga acggcggtgg cggttggagt
     3361 gagagaaagg tttttcaatt gagaaggcac ttgtgtcttc ctaaaaaggt cttttcctgt
     3421 ttctatgtaa aacaaaaaga gctgcaatcc tcatttagga ctacaactct tcttttattt
     3481 atatgcccat cgatcgcgta aaatgatcgg gatttctaac atacatgcta gcaataacta
     3541 gctgatttcg tttattgaag tgacctgtga cggcaatatt gtattttcca tcatccataa
     3601 ttagcagttt atcgataatt tcaatatctg caactacaca attaatcgat tcatgaattg
     3661 tttgtaaagt aaagcgaacc aataatggtt gtgtttttat tactcggatt ttatcaagca
     3721 aaccaacata tttgttcatc acgatcacct cgctaaaatt atagcaaaaa cagctaaaat
     3781 tgagaatttt tattcgattt tagatatgct ataatgatca agacagtaaa gtttgtgacg
     3841 gtggctatcc gattgaaagt gaggtggtgc gtatgtgcgt acttccaaaa tcttacagaa
     3901 aggagaagcc ttttgcctgt tgaaacggcg ctaggattaa tgattagttt cggttctttg
     3961 atcgcaacaa tcatttttgg aatcttagca gttgtaaaca acaatcaaaa aaaataaccg
     4021 tcagctttag ccggtcgacg attatttttc aaaaataata ttaagctggt caccgtctta
     4081 aacggtgctg ttaggggagt tgttagcgca actcctcttt tcgtatccat tataacaact
     4141 ttattgatct tttacaatca gaatggtaaa tcatcatcac taatatcgat tgattgtccg
     4201 ccaaatggat tttgtgaagc gttcaatcca ttgttgttag gcgtagctac atcccgttgt
     4261 tcaaaggtgg tagggcttgg actgctggac gattgatcag cgtgcgcacg gctctctgtc
     4321 gttgctttac tttctaatag ttgaaagtta tcagctacaa cttctgtcac atacacacgt
     4381 tgtccttgtt ggttatcgta ggttcgggtt tgaatccgtc cgacaacgcc aatcaagctg
     4441 ccttttttag ctaatgtcgc catcgtttct gctggtttgc gccaaatcac acagttgaca
     4501 aaatcggctt ctcgattgcc gttttgattc gtgaaattac gattaacggc taaggtaaaa
     4561 gtggccactg ctgtgccact tgcggtataa cgtaaatctg gatctttggt taaccgtcct
     4621 actaaagtta cattattaat catctttatc tctccatttc ttgattcatt tgttgtgatc
     4681 ttttaaactg cctttttctt tcttgatttt caattttgat cacacttttc attaggtgat
     4741 caatttcgtt gactgtatct gcaaatgaaa tggaattcgc aaacgtgtta actgagcgat
     4801 atttttccca ctgcttttcc aaatagtcgt ttcccctgat cttatccatt tctctttcat
     4861 aataaaagga taaaggcttt gtatctccac gttttgtaaa tgtatttcga acacttttgt
     4921 ataactcttt cgtatcaata ttttctagtt tcggaatggt aaataagtcg tagtaatctt
     4981 ttgctctcga attatctgtg ccaaagctga acgtagcact tagcttttct gcaataattt
     5041 gttctgttgg ataagctgga atctccactt ttttatcttc aaacattaat tggtgatagt
     5101 gtgtatctac ttcaggataa atagaatctc ctgttgtcac atccatttta aacggtacac
     5161 gtactttctc taaattagcc accaatttaa tttgaaatcc aggataaaaa tcggcttctc
     5221 ttgtttcctt cattccttga atagagaaaa caatgccttc ttttgtgggt tttctagtta
     5281 aatcattaaa aatgctcttc aatttttctt cagttaattt gctatttcta aatgtcgtat
     5341 cgatatcaat tgttgtcctt ctatcgattc catattttga tcctaataaa aagccgcctt
     5401 tcaaaatgaa gtcatttttg tactttgatt cagaaatttg ttcaattaga cgatccatca
     5461 taaaatgccg ttgcactaac tgtgcattta atcctgtact ctttgctaaa tttcttgttt
     5521 ttgctgcaaa ttgtgtcggt gtcatctttt tcctccatca atataagctt tgcatgtggt
     5581 atttcatttc tttttctaca tgtagttttg tcatgtaatc tcttaattta cttggatctc
     5641 tcaatgggtc ttccatgtac tcttttaatg cttctaattt catttcatat tcaatttcat
     5701 atctcggatt ccaaatatca caaagtgtac gctctttatc atacatagta ataggatttc
     5761 cttgaaagga agttccctct acttttccta gttcataaac ttctttcttc acgaaatgaa
     5821 attgtaactg ctgctctttt gtaattgaac gatcaacgtt atagccatgg ggaaaagtta
     5881 agtgaatgaa tttcggcata ttcgtagaaa gctggtaaat atctaaagtc gtttcatgag
     5941 agaagattcc cctggaaaat cgttgctgaa gccaaaataa atcatctatt ccatgaccat
     6001 cctctacata ataaccacga tcaactttga tcaattttcc ttgattcacc aatctttgta
     6061 cagtggttgc tgcgattcct aattttctgg cttccttcat agataaattt ccatcgcgaa
     6121 tagccaacaa ttctttcagt ttctcttgca tttacttccc cccttttgtt acaaatacac
     6181 tacattaaat atatcatgat agtgtatttg taacaaggtt aatgtctatc gatctaattc
     6241 gatttctttt tgaatcctct gctggttctt caattgctgc agtcttcgtt tgctggcacg
     6301 cattttttcc tgttctgaac gaattttggc tttttgattt tcgatttctt gttgtttgtg
     6361 tgtggtatta cgcataagat taaccctcca ttattttgca gtgcggcgtg ttcttttctt
     6421 gaagttcgtt tttttctttt cagaaaattc aaagcctgta gtgaagtctt cttttaaaac
     6481 aatcgttgct tcaaatgaac ccttctgacc ttttaaccct ttcactggtt ggcttgttcg
     6541 gctattttga atgatctcct tcagctgtgt cgctgtgagt ttctttttgg caatcgttgt
     6601 ccataattta aaatcacagg cttcagacgt acaagctgcc accttttgat agagtaagac
     6661 ttcttgttcc tggcattttg gacaaattcc taaatgacgc gcggcttctt gcttttcact
     6721 ggctgctttc acttcttgta acgtcgatcc aaaattagtg gattggattt gccctggtac
     6781 gacttccaat aagtgagaga caaatttttg gatattggct agaaaagtgg tttgtgttcc
     6841 ttttcgttca ccgatcttta acaagtagct ttcccatttg gcggtcattt cggcgctcgt
     6901 aagcagcggc tctttttcca cggcttggca aagcaaaatc cctttattcg tcaccacgag
     6961 cttatttttc tctaccttga tatattcctt ttgtttcaag gtttcaataa tattcgcccg
     7021 tgtcgcttct gtaccgattc cttccacttc tttcaatatg gattgcgctt cttcactatc
     7081 cacggttttc ccagctgttt tcattgcagt gatcaacgta ccttcggtat aaggttgtgg
     7141 tggcttggtt tctttttcaa gaacttgtag atccaccgtc accatttctc cttttgtcac
     7201 tttaggaagt agcggctctt cttccttctc ttttccgtcg ctctcttttc caaataactt
     7261 cttccaacct agatctagcg gcacctttcc gattgccttt a
//
LOCUS       DAJSMI010000131         7279 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJSMI010000131 DAJSMI010000000
VERSION     DAJSMI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.132"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..585
                     /locus_tag="ON430_002197"
     CDS             <1..585
                     /locus_tag="ON430_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220101.1"
                     /translation="DRLKKQESLKKINDQSDESTNLTALRNQNRRTGQRSKKRETGHQ
                     TLMKRQGEKVLQPINKPKDMKKQVVARSQRRTGQPTTRQNIQPKVAAQMVKRSGGTLG
                     SNPAYRRPEVRKKMDQVKQVTQVDVPQTHSTPQRVQPKVNEPITRMNKHNTPIIKKEK
                     RTKQSPVVRRERKKPGTQKITNKPKGVRQPRARK"
     gene            572..2830
                     /locus_tag="ON430_002198"
     CDS             572..2830
                     /locus_tag="ON430_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HCR3220102.1"
                     /translation="MQGNNLFNNGLLQPKKPPIGQEQQKMTRYHQLRLVFGLVGVLLY
                     PFAILGSIPLLIGLAVDKRDKAANVFDMDYESFLKRNSIVFLSFSAVLFVINVFAFIL
                     WIPRGYLSAYLLFPLNLLHTALRFNWETIVALLIGSSGMGAIFLAFSSFVAKRKVISK
                     EDERKKITESKAYKDREKNKFEESQRFTDEQEEAYEEAVETVDIDKYKELSNQLLLGT
                     SEFGLPYIINFSEFNQHVLVPATTGSGKTTLLQLLVQHAVKFNFPVILIDGKGARDTL
                     ESMREIAKFYDKEVHAFTDDGDMRYNPVEHGNDISIRDKLVSLAETESVFYSGAAKAL
                     LQVTIQLLDEFKGAKVTLSGDTRTTETVERSLPFVQRFLLPRNVLHLFADAILPNNPK
                     LFEIEVEKKIQKPKKKSVKEGSEILPDSDLDKEEKEEDSQIKNSKFRNISQLGIAQKE
                     TETIVLNPETLDLDSYYLLLKRNLRYLPTDKETGENIKQKLFERLFIRYEHKDSPFYL
                     YATSEALQTNINMLLDSELGKLFDTKNAKNVLDVQEIVNQRKLVYVSFNGLIYKEYIR
                     TLAQMLVGDVNYFASEMYRKNVKREVLVIFDEPASYLNETFIDMVNKGRGAGVYGIFT
                     PQTMADIAKLGDKLMEQLVGNVNTLFIGKTNEKGEAEYWSETMGTYQDIDVTSVTEQE
                     DGYSDVGKSDWSGDRGTKRNVDRFKISPNKIKELRTGEFIIYRTAENVNLPPQKVYVR
                     NALNWLKNNSKI"
     gene            3352..4137
                     /locus_tag="ON430_002199"
     CDS             3352..4137
                     /locus_tag="ON430_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HCR3220103.1"
                     /translation="MKPLLYNYYIDYTKKDKTRLIILCLLHELRVISVQQLIDFFQIE
                     RLSAESTVYKHLNLLKTDGLIARSKNGMHTFYYLTKEGHNYIGGYYTLPKVPEYNLQH
                     HLQINDYLIKMLELCNKHPHLKAVVSERRKVYEVKDEKKNQKGVKYFVPDFIFMFLDS
                     IGREVEWQFEIELTLKTKRRYSQGVFPKYIKHLKNYEDARLIYVTPSSIIKEELDMFK
                     EYFIDKEGDEYKEVFDRLHVFSAEEFESELKRLLEKDKFINWE"
     gene            4143..4631
                     /locus_tag="ON430_002200"
     CDS             4143..4631
                     /locus_tag="ON430_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220104.1"
                     /translation="MRRIEKEFNKKLAGYERELKKLGCLDDETGLIPISKRRWHVIWW
                     QPVTLAKTIVRSYRLTLDNQNLCILGDVEITIYHDGTYGISKEAVPIFINDLLSLKKL
                     ITIFYGTPFNLNFEKIRCVNFNRYCVSIPEIYVEKFEVLINYLIILSSCLHEVKKHVE
                     YD"
     gene            5223..5489
                     /locus_tag="ON430_002201"
     CDS             5223..5489
                     /locus_tag="ON430_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220105.1"
                     /translation="MLTTKEKNRFKKMVEGNKTFHYSYVDRLRQDVRYYVNQCESAVK
                     ARESMEILEFIYSLFSDKELPAWYTKADLENDKKSIEKLERWAA"
     gene            5522..6016
                     /locus_tag="ON430_002202"
     CDS             5522..6016
                     /locus_tag="ON430_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HCR3220106.1"
                     /translation="MKFIKDVTTLEELKRVYKKLALKYHPDMGGTDKEMAQINNEYEA
                     LFDKLKNTHKNKEGEYYQKETTETPQEWQEIIHKLLTLKMENVSIEVIGAFLWVSGNT
                     KPYKEELKALNMKWSNNKKSWYLAPDGYKKRSKKKYDMSDIRNMYGSQVVKEEQVKQK
                     TLTA"
     gene            6114..6530
                     /locus_tag="ON430_002203"
     CDS             6114..6530
                     /locus_tag="ON430_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010710134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3220107.1"
                     /translation="MIKTSAVGRLTKSSELRVTSTGKAVANFALACKRERLDKDGNAQ
                     TTFIQCVIWGKPAEVLAKYTQKGSLIAVNGELQSRSYDDQQGQTHYVTELVVAGFEFL
                     ESKETVQNREQKQQMPEMANAEPFYGMDDMEPPAYH"
     gene            6554..7138
                     /locus_tag="ON430_002204"
     CDS             6554..7138
                     /locus_tag="ON430_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR3220108.1"
                     /translation="MKKLIGKKWLLLTAVATFLLSGCASLEQKAQDSVKEVTENVTQT
                     ISNDQRIPADFVRHVDGDTTVLKIDGKEQKVRFLLIDTPETVKPKTKVQPFGLEASKR
                     TKELLSNASEITFEYDQGDKTDRYGRALGYIFVDGTLLQKTLVSEGLARVAYIKEPNT
                     KYLSELEQAQEQAKNESLGIWSIPGYVTQRGFSK"
BASE COUNT         2776 a         1179 c         1419 g         1905 t
ORIGIN      
        1 gatcgcttga agaaacagga atcactcaaa aaaattaatg atcaaagcga cgaatcaacc
       61 aatttaacag ctttaagaaa tcaaaatcgg cgtactggac agcgaagtaa aaaacgcgaa
      121 acaggtcatc aaacacttat gaaacgacaa ggagaaaaag tacttcaacc aataaataaa
      181 ccaaaagata tgaaaaaaca agtggttgct cgtagtcaaa gacgaacagg acaaccaacg
      241 actcgtcaaa atatccaacc aaaagtagcg gcccaaatgg tcaaacgaag tggtggaacg
      301 ttgggaagta atcctgccta tcgacgtcca gaagtacgta aaaaaatgga tcaagtgaag
      361 caagtaacgc aagttgatgt acctcaaacg cattctacac cacaacgtgt acaacccaag
      421 gtaaatgagc caatcacacg tatgaataaa cataatacgc caataattaa gaaagaaaag
      481 agaactaaac aatcaccagt tgttcgcaga gaacgcaaga aacctggtac acagaagata
      541 acaaacaaac ctaaaggagt gagacaacct cgtgcaagga aataatttgt ttaacaatgg
      601 cttgctacag ccaaagaaac cgccaatagg gcaagaacaa caaaaaatga ctcgctacca
      661 ccaactccga ctagtctttg gattagtcgg agttctttta tatccttttg cgattttagg
      721 cagtattcca ttactaattg gtctagcggt tgataaaaga gacaaggctg caaatgtatt
      781 tgatatggat tatgagagtt ttttgaaacg aaatagtatt gtctttctta gtttttcagc
      841 tgtgttattt gtaataaatg tttttgcatt tatcctctgg attcccagag ggtatctgtc
      901 tgcgtatctt cttttcccct taaatcttct tcatacagca ctgcgtttta attgggagac
      961 aatagtcgct ttacttattg ggtcaagtgg aatgggagcc atttttttgg cattttcttc
     1021 atttgtggca aaacgaaaag tgatttcaaa ggaagacgaa cggaagaaaa taaccgaatc
     1081 aaaagcctac aaagaccgag aaaaaaataa atttgaagag tcgcaacgct tcacagatga
     1141 acaagaagaa gcgtatgaag aagcagtaga aacggttgat attgacaagt ataaagaact
     1201 atccaatcaa ttattgcttg gtacaagtga gttcgggttg ccgtatatca tcaacttttc
     1261 agagttcaat cagcatgttc ttgttcccgc aacgacaggt tcagggaaaa caacgttact
     1321 tcaattactt gtccaacacg cagtcaaatt taattttccg gtgatattga ttgatggaaa
     1381 aggcgctcga gatacattag aaagtatgcg agaaattgcc aaattttatg acaaagaagt
     1441 gcatgctttt acggatgatg gcgatatgag atacaaccca gtagagcatg gaaatgacat
     1501 atctattcga gataagttag tgtctttagc cgaaacagaa agtgtctttt attcaggcgc
     1561 agcgaaagct cttttgcaag taacaattca attgttagac gagtttaaag gagccaaggt
     1621 tactttatct ggagacactc gtacgacaga aaccgttgaa cgaagtctac catttgttca
     1681 acgattttta cttccgagaa atgttttaca cttatttgcg gatgcgattt taccaaacaa
     1741 tcccaaacta tttgagattg aagtagaaaa gaaaatacaa aaacctaaga aaaaatcagt
     1801 gaaagaagga agcgagatac tgcctgattc agaccttgat aaagaagaaa aagaggaaga
     1861 ttcacaaatt aagaacagta aatttagaaa catttcccaa ctaggaatag cacaaaaaga
     1921 aacggaaacg attgtcctta atccagaaac attagattta gactcgtact atttattact
     1981 aaaacgaaac ctaagatatt tgccaacaga caaagaaaca ggagaaaata tcaaacaaaa
     2041 attgttcgaa cgattattta ttcgttatga acataaagat tcaccgtttt atttgtatgc
     2101 cacatcagaa gcattacaga caaacattaa catgttattg gacagtgagt taggaaaatt
     2161 atttgatact aaaaacgcaa aaaatgttct cgatgtgcaa gaaattgtca atcaacgaaa
     2221 gttagtttac gtatcgttca atggattgat ttacaaagaa tatatccgca ctttagcgca
     2281 aatgttagtc ggagacgtaa attattttgc ttctgaaatg tatcgaaaaa atgtgaaacg
     2341 tgaagtcctt gtgatttttg atgaacctgc ttcttatcta aatgaaacgt ttattgatat
     2401 ggtaaataaa ggtagaggtg caggtgttta cggtattttc acccctcaaa cgatggcaga
     2461 tattgcaaaa ttaggcgata aattaatgga acaacttgtc ggaaatgtta atactttgtt
     2521 cattggaaag accaacgaaa aaggtgaagc cgagtattgg agtgaaacaa tgggaacgta
     2581 ccaagacatt gatgtaactt cagttacgga acaagaagat ggctattcag atgttggaaa
     2641 atctgattgg tcaggagata gaggaaccaa acgtaatgta gatcgcttca aaattagtcc
     2701 aaacaagata aaagaattgc gtacagggga gtttattatt tatcgtacag cagaaaatgt
     2761 aaaccttcca ccacagaaag tttacgtgcg aaacgcatta aattggctta aaaataatag
     2821 taagatttag aagaaatagc aaatgaaaaa cgagagtagc catagagtaa gcgtagagta
     2881 agtatagagt aggtatagaa taaaaaatct aggagagcgg tcctcggcgc ttggtataaa
     2941 aggctttctc cgcctgcaaa attcctgacc attttcgctc aacgtgggtt aaaaccccca
     3001 ttaagcgaaa atggtcagga attttgcaac tacgaaaaaa ataccaagcg ccgaggacaa
     3061 acagaataga gtaaaaaaat agaatgagta aggtgataga agtaataaaa attaaagaaa
     3121 aaagttcaaa agataaagtt caaaagatta acaactaccc cttgcgatag acgccccttc
     3181 accaaacctt gaagggacta tcgcccaaag caacaacctc ctaaatcctc caccaaaact
     3241 tggaggactt aaaagctgaa aaagaatttc tacacgttgt tccgaaactc tttttcaatg
     3301 aaacaaatag gctttgccta tttgtacttt ttatagaaat gcggtgatat tttgaaacca
     3361 ttactatata actattacat tgattacact aagaaagata aaacaaggct aattatttta
     3421 tgtctactcc atgaattaag agttatctca gttcaacaat taattgactt tttccaaatt
     3481 gaacggttat cagcagaaag cacagtttat aagcacttaa atttattaaa aacagatgga
     3541 ttaattgccc gatcaaaaaa tggaatgcac acgttctatt acctaacaaa agaagggcat
     3601 aattacattg gtgggtacta caccttgcca aaagttcctg aatacaattt acaacaccac
     3661 ttacaaatta atgattatct gataaaaatg ttggaacttt gtaataagca cccgcacctt
     3721 aaggcggtag tatcagaacg tcgaaaagta tatgaagtaa aggacgagaa gaaaaatcaa
     3781 aaaggcgtaa aatactttgt tcctgacttc atttttatgt ttttggatag tattggacga
     3841 gaagtagagt ggcaatttga aatagaatta acactaaaaa caaaacgacg ttatagtcag
     3901 ggtgtgtttc cgaaatacat taaacattta aaaaattatg aagatgcacg cttaatttat
     3961 gtaacaccta gttcaatcat taaagaagaa cttgatatgt ttaaagaata ttttattgac
     4021 aaagaagggg acgagtacaa agaagtgttt gatcgattgc atgttttttc agctgaagaa
     4081 tttgaaagtg aactgaaacg attactagaa aaagataaat tcattaattg ggagtgagtt
     4141 tcatgagaag aatagagaaa gaattcaaca agaagctagc cggttacgaa agagagttaa
     4201 aaaaattagg atgtctggat gatgaaacag gacttattcc cataagcaaa agacgatggc
     4261 atgtcatttg gtggcaacct gttacacttg caaaaacaat cgtgagatct tatcgattaa
     4321 cgctagataa tcaaaactta tgtattttag gagatgtaga aatcactatt tatcatgatg
     4381 gaacatatgg tatttctaaa gaagctgtcc caatttttat taatgactta ttgtctttaa
     4441 aaaagttaat tactattttc tatggaacac cttttaactt aaattttgaa aaaattcgct
     4501 gcgttaattt taacaggtat tgtgtctcta ttcctgaaat ttatgtcgag aaatttgaag
     4561 tattaataaa ctacttgata attttaagta gctgcttaca tgaagtaaaa aagcacgtag
     4621 aatatgatta acaagctttt cttctttcag aagaacacca atagattggt tgagttcgta
     4681 tacgctcacc taagagagga aaagaacgaa gtcaagcttc gttctttttt ctacgtcgga
     4741 gaaccgacgt agtgattcag acgcaaaaac catgcgtctg aatcaggccc gtttttagga
     4801 agggctagtg tgtttgattt atcagttctc acagccgtgt aatttctaat attccctaat
     4861 cgtatatcag tcactcggcg agtataacct aaaacataac ctgtcggagg tcaagggcaa
     4921 gtggcatcgg caagccgacc cttgtcctcc gacagaacat gttttctagt cggttatgcc
     4981 gaacgaccga tatacgaaaa gggaatctaa ccagaatttc ccctctgacc tttgaaaact
     5041 gaaaagcgaa atcaaacaaa caggccttcg tctaaaaacg agcctaatca aaccttaggg
     5101 gttgcacccc taacacccgg cgcctcactc tctcaaccga aaaggttggt tggaaaagcg
     5161 ttgacaaaaa catatttatt tattggttgt ttgaatataa aaaatgaaag aaggaaagca
     5221 aaatgctaac aacaaaagaa aaaaacagat ttaaaaaaat ggtagaagga aacaaaacgt
     5281 ttcattattc ttatgtcgat agacttagac aagatgtaag atattacgtt aatcaatgtg
     5341 aatcagctgt taaagctaga gaaagcatgg aaatcttaga atttatctac agtctatttt
     5401 ctgataaaga gttaccagca tggtacacaa aagccgacct tgaaaatgat aaaaaatcaa
     5461 ttgaaaaatt ggaacgctgg gcagcttagc tgttcagcaa caatcagaaa gggcgattag
     5521 catgaaattt atcaaagatg taacgacatt agaagaattg aaacgcgtat ataaaaagct
     5581 agctttaaag taccaccctg acatgggcgg tactgataaa gaaatggcgc aaataaataa
     5641 cgagtatgaa gcgttgttcg ataagcttaa aaatacacac aaaaataaag aaggtgagta
     5701 ctaccaaaaa gaaacgacag aaacaccgca agaatggcaa gaaatcatac acaagttgtt
     5761 aacgctaaaa atggaaaatg tttcaattga agtgataggc gcattcttat gggtatcagg
     5821 gaacacaaag ccttataaag aagagttaaa agctttaaat atgaaatgga gcaacaacaa
     5881 aaagtcctgg tatttagccc ctgatggata taaaaaacgt agtaagaaaa aatacgatat
     5941 gagcgatatt cgcaatatgt atggttcgca ggtagtgaaa gaagaacaag tcaaacaaaa
     6001 gacattaaca gcgtaaatag gaattgaatt acattaggaa aaaattaggt tctacgtcca
     6061 aagaggtttt acctctttgg acgaccaaat aaaaaagaaa gaaggaatca aaaatgatta
     6121 aaacaagtgc agtaggaaga ttaacaaaaa gttcagaact aagagtaacg tcaacaggga
     6181 aggccgttgc taattttgca ttagcttgca aaagagaaag acttgataaa gacgggaatg
     6241 cccaaacgac ttttattcaa tgtgtaatct gggggaaacc tgcagaggtg ttagcaaaat
     6301 atacgcaaaa aggttcatta attgcagtaa atggtgaatt acaatcacgt tcttatgatg
     6361 atcaacaagg acaaacgcat tatgttacag aattagtcgt agcaggcttt gaatttttag
     6421 aatcaaaaga aactgtacaa aatagagaac aaaaacaaca aatgccagaa atggcgaacg
     6481 ccgaaccgtt ttatggtatg gatgatatgg aaccgcctgc ttatcattaa aatttatctg
     6541 aaggagggag aaaatgaaaa agttaatcgg taaaaagtgg ctgctgctta cagcagtagc
     6601 cactttttta ttatcaggat gcgcaagtct tgaacaaaaa gcacaggata gtgtaaaaga
     6661 agttactgaa aatgttactc aaactatttc aaacgatcaa cgtataccag ctgattttgt
     6721 taggcacgtg gatggcgata ccacagtatt aaaaattgac ggaaaagaac aaaaagttcg
     6781 gtttttatta attgacacac ccgagactgt gaaaccgaaa acaaaagttc agccgttcgg
     6841 attggaagct agcaaacgta caaaagagct tttgtctaat gcttcagaaa ttacttttga
     6901 atatgatcaa ggcgataaaa cagatcgcta cggccgagcg ttgggttata tttttgtaga
     6961 tggaacatta ctacaaaaaa cacttgtgag tgaaggttta gcacgtgtgg cctatataaa
     7021 agaacctaac actaaatatt tgtcagaact agagcaagcc caagaacagg ctaaaaatga
     7081 gtcactcgga atctggagca taccaggtta tgtgacacaa cgggggttta gtaaataaat
     7141 aagaaatcgc tatgaattat tgaaagtttt tggcgagaaa ttataacaca ttatgttgtt
     7201 gttaacaaca taatgtgtta taattatata cataaaggag gggtaaaggt ggctactaca
     7261 aaaaagaaac ctatacatg
//
LOCUS       DAJSMI010000132         7265 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJSMI010000132 DAJSMI010000000
VERSION     DAJSMI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.133"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(16..213)
                     /locus_tag="ON430_002205"
     CDS             complement(16..213)
                     /locus_tag="ON430_002205"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR3220109.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(359..1237)
                     /locus_tag="ON430_002206"
     CDS             complement(359..1237)
                     /locus_tag="ON430_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3220110.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1276..2394)
                     /locus_tag="ON430_002207"
     CDS             complement(1276..2394)
                     /locus_tag="ON430_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR3220111.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2391..4490)
                     /locus_tag="ON430_002208"
     CDS             complement(2391..4490)
                     /locus_tag="ON430_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCR3220112.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4541..5362)
                     /locus_tag="ON430_002209"
     CDS             complement(4541..5362)
                     /locus_tag="ON430_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3220113.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5359..6168)
                     /locus_tag="ON430_002210"
     CDS             complement(5359..6168)
                     /locus_tag="ON430_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3220114.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6189..6683)
                     /locus_tag="ON430_002211"
     CDS             complement(6189..6683)
                     /locus_tag="ON430_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3220115.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6695..7183)
                     /locus_tag="ON430_002212"
     CDS             complement(6695..7183)
                     /locus_tag="ON430_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3220116.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
BASE COUNT         2207 a         1680 c         1215 g         2163 t
ORIGIN      
        1 ggctgaaaaa atctatcacc aaaaaaattc aaacattaag ccaaggatta tgaagtcaac
       61 atttcgaaaa atgaacatac attggtaagc aactataact attaccacag acataaagta
      121 acacaatata ttactattta agtgtacgcc accagtgaat atcctcaagg tatacagcgc
      181 acaacaaaaa gaaaaaagga aacgcagaag catttccttt cagtgattta atgaaaacaa
      241 cacgatttga cttagagcct ttttatttga attcattttc gctttatcca acttcatttg
      301 atcatttgat tactcggtta aatacttttg acatgagtat caatgaaaca ttcattaatt
      361 atggttgctt atttcttacc gcttttctgg ccaatgctaa tccgccgatt attccggcat
      421 tatcacctaa ttcgggcgta ataatgtagc tatcaattgg tggtatttca acatatccat
      481 ttataagctc aacaaattta tcctgaactt ttttcttcat gtgttcttgt ttcattacac
      541 caccgccaaa aataattcta tctggcgaaa acatcagtgt tgtattgtat gcacattggg
      601 caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc caataaattc tggcctttta
      661 cacccaaccg tttttcaatc gctggtcctg ctgccatccc ctctaaacaa tcttggtgga
      721 agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata tgccccattt
      781 ccggatgact gaacccttcc acgaaagtcc cattgaacaa ggcaccacct ccgatacctg
      841 ttcccacagt gaagtatgca acacctgcta accctttgcc agaaccagca acatattcac
      901 catagcaagc cgcattgaca tctgttgtcc attcaatcgg aatattgaaa tattgcttta
      961 gcgttcctac aaaattgaaa ttttgccaag caagttttgg tgttgatgtg atatgaccga
     1021 aactcgttga tttgcattga atatcaattg gaccaaagca gccgaccccg atgcctgcca
     1081 attgttcctt aaaaggttta aaaaaatcaa tgacctgctt cattgtttca actggagttg
     1141 ttgtgggaaa actgactctt tccaaaatcg ttaattcctc agtaccgact gcacagacaa
     1201 atttagtccc tccagcttca attgccccat atatcatcat aggttcctcc cttcgaatat
     1261 gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt
     1321 tcctgaagct tctgttgaat accagtaagc gacactagca acatcgtctt gccgctcaaa
     1381 taagcgaaca tcatcgttgc cgatttgttg taaagtgacc gataaatctt cggtaaaaca
     1441 gatggggtct tgaagatgcc aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa
     1501 ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct cttgtggcat ctctatttga
     1561 ttggtaagga taccctaaaa aggctgttga atagtttttc gcatgtggac gtccgaactc
     1621 atcgtactca tgaaaggccc acgccccgcc aaaataatct tctgaacctg ttgaggttac
     1681 ggtcggatac tcactatccc catcaatgta gaatttaaac tcgccttcgc cccaccaata
     1741 acgttctagg caggtaagcg caaggtaagt acccacgtaa tagccttgac cctttaatcg
     1801 atcaattaac ttataatcct cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg
     1861 aaaatatagg ctattttctt ccaattcgtc caccaaagcg taatttatcg tataaaaaaa
     1921 tgaggacata tccttcggat gtaaattctc gatcgtaatt ttagcggatg tttggaaagg
     1981 catttcgaaa taggaattaa agccccctgt aggattaacc actatcggca acgagttcac
     2041 ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct aaaggacttt caacagcagg
     2101 gctatccgac ccatcccaat aaattcttaa gattaaatca cgtaaaacaa aactgccaaa
     2161 ttctgttcga tcacgaatcg tcatccacat atgacgaatt attcccgttc cagtaatatc
     2221 cgcaatcaca gtttcttcac cagaagtcag cggaattgcg ccacgacctt tgcggcttgg
     2281 tcctagctcg cttggcaaca tcgcagcttt acctttctcg cctgttggat tttcgggagt
     2341 gattgctcga ctctggattt ttttaaattg ggtaatattt tttagtatac tcatcttttt
     2401 ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa gaattttttt cgtatcactt
     2461 attttcaatg gcttttctga caaatttgaa atactcaact cggattgcgt acattcaatc
     2521 gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa acgttaaatt atcaacagga
     2581 tcaatttgca atactccttt tttgaatttc aatccgaaat attgactgat cattaggctg
     2641 acaaaaagac ccgatgccca gccgcatttg ccgcagccaa agtctcgcac gacttctcct
     2701 tgttgagcct ctaagcgata cggccaccac caccacgaac cgtctaaatc tgccaaacaa
     2761 attagttctt ctaatcgggt tacgaaagct tgagggttgt gcagctcgct cattaaaaca
     2821 gtgataaagc caggaaaagt ggcgccggat tctaatcccc aagtgatgcc tttgatttcc
     2881 tcgctatagg ttggattgaa agaactagca gaaaaggcca tggtgttctg atataaatga
     2941 tctttattat ctaaaaattg ataaaaaggc atcaacgtag tgtctgattc ttctccatca
     3001 tgcatcaata aattaatttt tccatccaca atcacatcac ttaaaaagat taacccttgc
     3061 tcaagaatcg gtttttgata gtgcttcact gaataggttg ttttctcctc atcaccgatt
     3121 ccctccaaaa attgctcacc aaaaggacca tcgatagtca tttgtttaca aattgattcc
     3181 tttagtttac aggcaatgtt tttgtatcgc ttgctaagac tcagttgttc tagggcttca
     3241 aaaatttctg caagtccaat acaggccttc caaagacaaa gattgctgcc ggtatgatac
     3301 tttccaagtg caaaggcgtc tgataaccaa acagatggaa aaagcattgg ttcccctgac
     3361 cgacgttgag ctaatatgtc ctcaataatt cttgaagcat tcagtagaac ctgcggacac
     3421 cgctcaaaga attttgtatc cccagtatgt tcaaaataaa gactagccag taacgcactg
     3481 gataacgaat tactcagaga atgattgata cctcctgtaa attttgaacc aggaaactta
     3541 acgccatatt gatcaaacca tagaaccgtt ttttgacata actccggttc aaacatggtg
     3601 aatggcaatg atgcgtaata catatcctta ttccaaattc gattagtagc aggatagctg
     3661 ccccaattgc tgccgacgac ctgtccctgt cgattcatac cgaaactaga aaagctttga
     3721 taaagtgcac gacgatagag atggacaact ctatcgtttg gcattgacag ctgcccaaat
     3781 agctgagcaa aatagttcaa tgtttccttc agccattttt cgatatcaga ttcctgaaaa
     3841 gtcattactt cttcaaaagc atttgggtcc acaaaagcaa tggcgaagct tgccacttcc
     3901 tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca acacattttg ctgatccgaa
     3961 tacttctctt gatacgaagg cacatctttc actgaaactt gaaggctttc gtgtgtttca
     4021 ttcacaatgt aaacctgttg aacaagcata cttaaacgct tattagccag tatgggagca
     4081 aaggatacta caatgacctt aaacgtcgaa taatgatgaa tatatctagg caaacagtct
     4141 tgaatcacat cgacttcaat acgatgatcc gattctgaca aatggtagac aatcccgttt
     4201 acttcaacct ttaaggataa cgaattggtt tgagagagct gtttattgac ccaaacacct
     4261 ggtttttcct cagttagtaa attttctcga taaaaggtca acgtttgaat cgtcccgcaa
     4321 acatcaaggt ttgctaatgt cttttgatta cttacatcga aggtcaatcc ctttacatca
     4381 ggaatattcc gatagtagct gtattcgttt gaagtagtca catttccgtc gtttaactca
     4441 gcattaaaaa atgggtgttt gggtgaataa attacttcat taatagtcat cttttccctc
     4501 cttgctaaaa aaactgcagc ctagtagctg cagcttccta ctatgcgatt ccaagtaatg
     4561 acaataaaat tcccaagacg accattccaa aaataatttt gatgacgccg acttttttat
     4621 ctagtaattt gaaacagctc aatgttacca ataacggtac aattccttta aaaatactat
     4681 cgaggatttc ttggacattc attagttctt cgcccttcat tgaaaagctc aaaacggtat
     4741 tgaatttgac catactgatc gtcattgcac ctaccatgat cagacccaag atactagcag
     4801 cttttgtcag aatttcaatt aacccgttcg catacagctt ttcaatatat ttagagccta
     4861 atgaatagcc taaaacgccg ccatagtatc gcatcaagta attaggtata ttgaacataa
     4921 ctaaaaagat aattggtgcc aaaatatttc cggcattggc taagttgatc gctagtcccg
     4981 cggtaacgac acgccaaact ccccagaaca atgaatcgcc aattccagcc aatggtccca
     5041 tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa accagctttt tcagaatttt
     5101 ccttctccat cgatgccgca atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa
     5161 aatactgtgt atgacgttcc agcgccgcag ctcgatcttc ttcacttttg tagaaatgat
     5221 taatgaaggg catcatagaa taggcgaaac caccgccgcc catcgtcacc ggattcaccg
     5281 aagcatacat agtcatcgag cgccaaaaca tcttccgaac caattttttc tcatctttag
     5341 aaattccatc agctaatttc attgaaagaa atcctcctct tcttggtcaa gcgttgcatt
     5401 tacagcagca tcgctttgaa ctgcatgaac agatatattt tttagttgta gatctctttg
     5461 ggctataaca attgcaataa tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag
     5521 atacgccata acaacggccc caaagaaaaa ccacacagca atatttttac tccaaagcat
     5581 ccgtaacagc atcgccatcc caactgccgg cagcagattt ccagcgactg ttaatccgtt
     5641 catgatgacc tctggaatat tatccaagac gcttttgaca acatctgctc caaataaaac
     5701 ggcaaagaat ggaataagag cataaagcgc atagtggaca aaccacataa agaagtgcag
     5761 attgatgatt cctttttgat caccagattt tgcaaatttg aaaaatttag cagccatcgg
     5821 tccacataca aaagaaaaaa tcatattcat taactgtaat cctaatactg ctagtggaaa
     5881 gacaatggga ataatcgcat ccacgtttcc tcccattttg attgcgaacg ctgtagccag
     5941 cccagcagcc aaagaaggct ccgccgtact agagattccg atattgacga cacccataaa
     6001 gatggcttct agtgacgccc cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc
     6061 tgctaacgga gcaacaacaa tcggacgact cagcatggac atcccgaaca tttcatgtcc
     6121 agcaactcca ataaaaacgg ccaaagcgac tacaaatgca tcatacataa tttttctcct
     6181 tcctttttct aaaaatcact caaaatagag atagctgaac gtttttccat cgccggtgta
     6241 ggctgaaaaa cggtatctgg ataaagttca actaattctt ttaagtcttg aatttctgac
     6301 gtcgttaaca tcacttcctt tgataaggtt ttcttatctt ccgatggcgg tgtcattttc
     6361 ccagcattcc ccacgttcat taattcgatt tccgttggga aagctttcgc aacttttacc
     6421 gcatctttga cattagggac taaaaccatc aagcgcatgt ccttcgcccg cggatctttt
     6481 aacaaatgaa tcgcgtcatc aactgtttta ataatgactt tgacattcga tggtgcagcc
     6541 atcttcaatg tcatcttttg aatatcgttg gtagccgcct catcgcttgc tactactaac
     6601 ccggttaaat ttagactctt tgtccaagtc atcgctactt gtccatgaat taatcgttca
     6661 tcaattcgca ctgctttaat catttttctt cctcctaaaa aaaatcttcc tcttcattta
     6721 ctacatgtac ctcatcaaga atggtcatct ttaattcctg ttggcattta tttaccagtt
     6781 cctgaatatc ctcctttgtt aattccttgg ttgccaatac tatttcgagc agaattggca
     6841 gattaaagcc agacaaaata gttacagcaa tctctttctc caaaaaggtt ttggtaatct
     6901 tctgattgac acttccgcca aataaatcgg taaaaacaaa aatctgatcc tgctcgttca
     6961 cctggtttac aaatacattc aactcctgtt caaaatcaca ctcatccaca taagcattaa
     7021 tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat actgctaaga aagcctgaag
     7081 ctaagtgacc gtgagtcgct attagaattt tcttcatatg gttaattcct ttctttctgt
     7141 tcacttttat ataagcaatt cctgtgccaa cttttaaaaa cattggttta atcacgttaa
     7201 ttcacattaa aaaaagtgtc taacctaaaa aaaacagatt agacactttt ttagacactt
     7261 tattt
//
LOCUS       DAJSMI010000133         7219 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJSMI010000133 DAJSMI010000000
VERSION     DAJSMI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.134"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..819)
                     /locus_tag="ON430_002213"
     CDS             complement(<1..819)
                     /locus_tag="ON430_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3220117.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKN"
     gene            complement(792..1472)
                     /locus_tag="ON430_002214"
     CDS             complement(792..1472)
                     /locus_tag="ON430_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3220118.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1495..2433)
                     /locus_tag="ON430_002215"
     CDS             complement(1495..2433)
                     /locus_tag="ON430_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCR3220119.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2678..3541)
                     /locus_tag="ON430_002216"
     CDS             complement(2678..3541)
                     /locus_tag="ON430_002216"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR3220120.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3683..6256)
                     /gene="mprF"
                     /locus_tag="ON430_002217"
     CDS             complement(3683..6256)
                     /gene="mprF"
                     /locus_tag="ON430_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR3220121.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6397..7203
                     /gene="proC"
                     /locus_tag="ON430_002218"
     CDS             6397..7203
                     /gene="proC"
                     /locus_tag="ON430_002218"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR3220122.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT         2210 a         1589 c         1245 g         2175 t
ORIGIN      
        1 attttttctt ctgaaggcaa ccaatattcg tttgatgata tttacaatca ggaacaaagc
       61 aataaagact aatgtgattg ttgctcctgg aggtgtatct agataaaacg agctagttag
      121 tcctgacagc atccctagaa atcctacaca aacactgata gcaatcacga cattgaagct
      181 tttgcctaat cgcataccga ttgctgcggg aagaaccatg attgcggata tcaaaagtgc
      241 accggcaatc gggatcatca cagcaatagc cacaccagta atcacattga acatcatcga
      301 catcaaatga acgggtagcc catccacgtg ggcggtatct tcatcaaaag tcagaacata
      361 catcggccgt ttgaataaaa agaacaatat accgatggca acgaataaga ttcctaaaaa
      421 gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac agataggatt ggatacttgt
      481 tgctgaattc cctccgctca aattcatcaa tactaacgct agcgccaaac caccagccat
      541 taaaatggca atggagattt ctgaataggt tcggtagatc gttcgcagat actccaatat
      601 cacagctgct aatacaacga ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa
      661 tcctaacgct acccctgcta aagagacatg agaaagcgta tcagccatca gcgattgtct
      721 tctgatcact aaaaacacac ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag
      781 aaacgctctt ctcatgaatt catatgaaag caacgccatt gagaatcctc ctttctcacc
      841 agtcggatct gtctgtccgc atactgtttg atatcttcat gatcatgggt gatcattaga
      901 actgatttcc catggacatg cgcactatgt cggagaagac gataaaattc atttctggat
      961 tcttcatcca tacctgttgt tggttcatca agaacaaata agtcagggtc agtagcaaaa
     1021 atacgcgcta aactgattcg ctgcttttgt ccgccagaca attcacctat tcgccgattt
     1081 cgcatatccc acatccctac agcttctaaa gcacgctgta catgttgatg atctttttca
     1141 gagagacgct tgaaccagcg gcctcttgga tatcttcctg aacggactaa ttcgatcacc
     1201 gtactaggaa aacctgcatt gaaagatgca acttgttgag gaatataacc gatacttaat
     1261 ttttttcctt cttgattttt ctcagcaatc tttatcattc cttttgaagg tttcagtaat
     1321 cctaacgatg ctttcaccaa ggttgacttg gctgctccat tttctccagt taaaatgaca
     1381 aattcacccg catctacatg ataagagaca tcttctagta caggttcatc atcatagtaa
     1441 aaggtcaaat cagatatttc gatataacgc accttacaac ctccattcta aaacttaatt
     1501 gatactttct tttaaagctt tcagattttt ttccatgaca gaaatataat tttcgcctgc
     1561 tttgatttgt tcattagtca agctttctaa aggattcaac acttctagct tcactcctgt
     1621 ttcattcgac aatgtctcag ctaccttcga agaggcattt tcttcaaaat aaatcacttt
     1681 gatgttgttt ttcttcacaa attcttttag ctcagctaag cgacttggtg tgggttcctg
     1741 atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg tattgattcg ctaaataagc
     1801 aaaagcagca tgctgagtga caaatgtgcg gtttgttgca tcttttagct catcggagta
     1861 tttttgatcc agtgcttcaa gctttttgat ataagcatcg gcattcttcg taaaagtttc
     1921 ctttttatct ggatattttt cgcctaattc ttcagcaatc gttctgactt cttttatagc
     1981 taataccgga tcgagccaaa catgtggatc gtattcatgt gaatgaccat gcccttcttc
     2041 tgattcttca tgttcgtctg cttcttgcga ttctaaacgg tcgattcctt tagctgcttc
     2101 gatcatcaag gtctgtttgc tatccactga atcctttaac gaatccacaa aaaattccat
     2161 gtctgaacta ttatacacga aaacatctgc atcggaaatt tctgctcttt cttttgcact
     2221 tggttcgaaa tcatgcggct ccgttccagc agggatcaaa agtttcacgt ttccttcatc
     2281 ccctacgatc tcttttgtaa aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc
     2341 ctcttttttg tctgcttcct tgttcgtatt tccacatgct gcaaacagta aagcacctat
     2401 cattaccgta gctgtcaaaa gatatttttt catttatatg ctcctttcaa tgaacaaatc
     2461 ggaattttac gatttattaa cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag
     2521 aaatccatca atttctgatt taagcatgta tattcttgat taaatcgtaa aatggatgag
     2581 ttgctgctca tatctagcca tttcactaca ataaaaaact gaagaatacc attttaggat
     2641 tcctcagttt ttcatcacct attttacttc ttatatttta ttgcagggtt cgtactaaat
     2701 aaacttcctc acaaaaacct tttaacccat tatatactct ctgcgctctt gaataatgtt
     2761 gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa tgcagcatct gctccgtact
     2821 tgatcatacg ctctttgatt tgatggatga ctggatgccg ctttcccgtt atatcttcta
     2881 aactgtttcc catttttttg atcatctgct gataatcttg ctctcggatg gcttcagcaa
     2941 gtccgtggat gtcttgatgt tggatggctt gcgtatctac ttctctaaac actgtacgtg
     3001 tagagacact cattcttggt ttgaccaaca caacccagca ctgtggcata gaaggtaata
     3061 cttggatttc ttcccctttg ccaccgacaa atgccgtact tccgtagaca cagtaaggaa
     3121 catctgtacc gacttccaaa ccgatttctg ccagttcttc taacgaacag cccagattcc
     3181 ataattggtt gatccctctt agagcagctg cacagtctgt acttcctccg cctaatccag
     3241 ctgctactgg aatattcttc ttaacaaaga tcttgacacc ggttttgata ttgtatttat
     3301 tttttagcaa taaagcagct tgataaacat tgttccgttc atctaccggt aaaaatgctt
     3361 tatttgtttc tacaatgatt ttgtcttctg ttatttcttc aaaaaataga tgatctgcca
     3421 agtctacgct cgacatcacc atttccaact catgataccc atcttgccgt ctatataata
     3481 catccagtcc taaatttatc ttggctggcg ctttttcaat caattccatc ctctcaccca
     3541 ttcgaaaagt ctaagtcgca agtattctat catagggaaa aataaaagta tagcttatgt
     3601 ctgcctagtg gttgattttg tcatcgtttc aacagctttc acagaatttt ctcttcataa
     3661 tccaaacaag ttctagtgat tcttataatt ctgtgagcac ttcttctgcc aaactcggaa
     3721 catttccttt ttcttttcct atcagactga gtagctgcat ggtagcaaat ccgaaattac
     3781 ttcctttcct ataagcaaaa taacgtcctt cccaatgatc gacatatttt aatttctttt
     3841 cataagcaga ctggaaggaa tagaaaaaat taccataatt atatacgata tgcatgactt
     3901 tttcttgaaa gaaagaaaaa tctgtttctc caacattagc taatggagct aagcctagat
     3961 tggcttccga gtagcccatt ttctcgtata caataaacag attggctaac agatagtcag
     4021 ctaattcctc agattcttta ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg
     4081 tcatcggctg agcggtgatg aagccaacga ctttttgatc tttacggaca ataccgacac
     4141 cgctagataa tacatatgat ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac
     4201 cttgaagcca tttttgtgaa acttcattta cagcttgatg taattcttcc ggcagttgat
     4261 cgtaccaagt aaaatggtat ccttctttca tcaaacgttg atattcaagt tgctgactca
     4321 gccgagacaa gtcttctgtt gctagatcaa caatccctgc ttcacctact ttcgtgaagt
     4381 gaaaacccaa ttcatgaagc atcactacgt agctttctga tatcttataa aacgcaagct
     4441 gatagcctaa acaatctgct tgtttcataa actgattcgt tgctgcccgc atcattttag
     4501 gattgccaac tggatctcct aaaacaaagc atttgtttcc tttgatctga taaccaaaaa
     4561 cgacttgatc ttggtcgtct tcttggtaat aaaaataact gtaacctgac aagcgaagct
     4621 gatggcttgt agctgtgcct ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt
     4681 actcactgcc aagcttcgta gtatcttcag ctaaatattg ataaagcgcc actaaagcta
     4741 gcaatgagat gcccaaacca ataagccctg agaaccacac atcatctgaa ggaaacaaaa
     4801 taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg
     4861 cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa gattatagct ccccaagaat
     4921 aaacgaattt tgttcgataa aattcttttc tggataacca cacagctaac agaatcaaga
     4981 catataccaa tatcaactgc caagataacg tacgtgtgat tgtatttaaa ataccaaaag
     5041 atagtaaagc aatcgtaggc agaaatgcct ttttgacttt tactgaaagt gctctagcaa
     5101 ggccgagtaa taaaaagcca accaatagat tcactgtctg atctaaaaaa ttgaatgaga
     5161 acggcaataa tacttgaaat agccgactga ttgcagacag attagtgatt gttgatagaa
     5221 gtaccatcat gatccccgca aaatagacag cacttacgag cacgatatgc gcaacctttt
     5281 ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat tttgactcct gtatgtgtga
     5341 tgaacaatag aagtcccgtc ataaaaggta gaacataata aaacaaccga taatataata
     5401 gccaaactac tgctacatct tgccgaacag acaattgact cattcctaag atcatcaaaa
     5461 cgtcaaaagt ccccattcca cctgggacca tcgtcagcat tcctatcaat gtagcaatca
     5521 tgaacatagg atagatcgaa agcatcgaaa tatctattct catcaacttg ccgatgatca
     5581 aaaataccgc aagggctccc gtccattgtc caaaggatgc cagtagcaga ccaagcaagc
     5641 cttttggata aaactcgaag aacaatgtct ttctctttag ataagcaaaa aagaaaatga
     5701 ctggtgctaa aaaactaccg ccgaaaagcc agatccaata gcttcgaaaa atactttctg
     5761 actgaataaa aaagacatca ataaatgtaa gaaacgacca tacagacaaa ccagtcagca
     5821 taaatagtgc gacttttgat acagtggcta aaaccatttt tctatttgtc tgctttccat
     5881 aaaaactagc ccgcagactt gctcctacca caccaccaaa accggcgaga ttgttgatcg
     5941 tgtttgttac ccacgcagca agaagaaact cttttctttc catttttggt tttccttgtt
     6001 tttctaagac tctgatcgtc acccaatcat acaacaacat cggagtgaca cccagcagtc
     6061 cagccagaat catgagcagg atcgtcatgc ggctttgttg tcccatcctt tgccaaatat
     6121 cttgccaact catgccatta gcaatatttg cgacttgatt tgccacaaag actaacacgg
     6181 agccgaaaaa aatcagtttt aaaatcagac tatgttcctt tatccattgc agcatcaagt
     6241 atcgtttatt tttcacattt tcccattcct aacttgtata cttttgtgtc ataccccaat
     6301 gttatcttat ttattttaat ttttccactt tagacctgtt ctttgcttgc gatgatagaa
     6361 aaagaaagat atcctaatgc taaaggagag gataacatga aagtaggatt tatcggcaca
     6421 gggaatatgg cacaagctat tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa
     6481 gaggtgttcg tttccagtgg acattttgaa aatgcacaaa aatttgctgc tgaaactggc
     6541 gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat cagatatcgt tcttttagcc
     6601 gtgaaaccaa aaatcattga aaaagtattg gaagaactaa gagaatatag ccagaaagtc
     6661 ttattcattt ctattgcttc aggaaaatca ttaacagatt tagaaacagc gcttgaagat
     6721 cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt
     6781 tctgcgattt gcaaaaatga agctgccacg gaggaacagc ttcagcaagc tacgatgatt
     6841 tttgaagcaa tcggcagcgt ctatccttta gcagaatcag atttcagtac attcattggc
     6901 cttgctggca gctcacccgc attcgtttac atgctgatcg atgcgatggg acggactggt
     6961 gtactgaatg gtttgtccaa acagacagcc actgagattg ctgcaaaagc aattatgggt
     7021 tcttgtcaaa aattcctttc aagtgataaa aatccatggg aacttgtaga ccaagtttct
     7081 tctcctggcg gtacaactgt tgcaggtgta gttgccttag aagaagcagg attcatccca
     7141 gcaatcatca aaggaatcaa tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa
     7201 taatgatagc aatgttttt
//
LOCUS       DAJSMI010000134         7075 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJSMI010000134 DAJSMI010000000
VERSION     DAJSMI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.136"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="ON430_002219"
     CDS             complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="ON430_002219"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR3220123.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="ON430_002220"
     CDS             complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="ON430_002220"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR3220124.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="ON430_002221"
     CDS             complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="ON430_002221"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR3220125.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2590..3312)
                     /gene="tsaB"
                     /locus_tag="ON430_002222"
     CDS             complement(2590..3312)
                     /gene="tsaB"
                     /locus_tag="ON430_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR3220126.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(3503..4483)
                     /locus_tag="ON430_002223"
     CDS             complement(3503..4483)
                     /locus_tag="ON430_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3220127.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4507..5991)
                     /gene="galT"
                     /locus_tag="ON430_002224"
     CDS             complement(4507..5991)
                     /gene="galT"
                     /locus_tag="ON430_002224"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCR3220128.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6003..6992)
                     /gene="galE"
                     /locus_tag="ON430_002225"
     CDS             complement(6003..6992)
                     /gene="galE"
                     /locus_tag="ON430_002225"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR3220129.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         1921 a         1550 c         1257 g         2347 t
ORIGIN      
        1 aaatatcagg gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta
       61 gatcactgga caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt
      121 ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt gtcctcatcc gatctagatt
      181 attcgatatt cctcgacaat aaacgattac tcagtgaggg caatcagagg aacggataat
      241 gataaataag ctgtaaaaaa tagcaaaata acaataatga ttaaaagtat gtaaaaaata
      301 gggctttgac aaaaacgtgt cacagcccta tttcgattaa acggtgtgta cctgtcaact
      361 cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt
      421 gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg
      481 catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt
      541 tcttattact tgatgcaggg cagctttttc acaacctctt tattcagata aaaccaagct
      601 tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc
      661 tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac
      721 ctcagggagt ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc
      781 tacacctcca gctataatca gttgcttgac tggataattt tgacaagctc gaagtgtttt
      841 agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag
      901 atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct
      961 aaaactgaaa tcataattat cttcatggat catcgctcgc ggaaaatggt aattatcttt
     1021 tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct
     1081 tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata
     1141 tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat
     1201 caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt
     1261 tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat
     1321 cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc
     1381 cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg
     1441 gcggctagct acttcaggta ctacgccacc aaaacgttta tgactgttta tttgagaagc
     1501 aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca
     1561 acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt
     1621 ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat
     1681 atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct
     1741 aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt
     1801 gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct
     1861 attactttcg agtatccaat aaagccacag atctggttat cttcagctaa aagcagataa
     1921 tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt
     1981 ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct
     2041 ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca
     2101 tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa ccccaatttc
     2161 cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag
     2221 cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc
     2281 tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa
     2341 ccgataaaac caatcattct tccattatca aagataccta gatatagatg aggaagcggt
     2401 gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct
     2461 cgttcgacat ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga
     2521 cgatcgattt gttcgactgt tttttctgta taaatcattt tatcttgaaa aaagcttctg
     2581 gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca
     2641 tttttcttct gcttctactc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac
     2701 tggttcaact aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg
     2761 aactgtatta atcatcgcac taggcaatat ttcctttatt tcagagaaga atttgtgaca
     2821 atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga
     2881 agcagaaata tgtacatctt caagtacagt ttctaatcta ttttctttcc agcggtatgc
     2941 cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataatat tatccacacc
     3001 gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt
     3061 ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc
     3121 atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg
     3181 gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc
     3241 aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc
     3301 taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata
     3361 cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaataat acatgattat
     3421 ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa aagctgcggt atcactccat
     3481 tttcccacag cttttccatc ttttatttcg ttgattctcg aataatcaac tctgtttcca
     3541 caataatctt tcttgaaacc ggtgctttgg aattacttaa ttgaagaatc gtctccactg
     3601 ctagctctcc catccattcg gtataaactt ttacagtaga aagcgagggc gttacatatt
     3661 tcgctacact gacatcattg aagcccatca cagaaatatc ttctggtacc actaaacctt
     3721 tttcttgaat cgctttcagt gcgccaatcg ctaatgcatc attagctgca aaaagcgcat
     3781 caggaacatg cggattcgtt tttaaatact gcttcactgc ttgatatcct gactcgacag
     3841 aaaaatctgc ttcgataatt ggaccttcgt cgtagccttt ttctgccaaa acttgttgaa
     3901 atgccataat tctgggatct tttagccgct gatggttttt cttcgtatat tctgttccag
     3961 caagcatagc gatatgttgc gctttatggg agacaaagtg ttcaatgact ttttgaacac
     4021 ttgacttaaa gtcgacaact agagagtcga ttccttgaga agtgccgtca gaatctacca
     4081 aaagcaaggc attttgggat cctttcattt ctaataactg agcttcgtcg aattttccta
     4141 gtgcgatcgt tccatctgcc ggaagatcac taatggaatc ccacgtttct ttcaataaag
     4201 taacgtttgc ttcttctgcc tttttttcga ttcctaggcg aatagataaa taataaagat
     4261 cttcaagctc ttccgtatcg tcgtaccact ggatcaggcg aagcgtttgc ttagtatttg
     4321 ctgcattttt acgatgtttc gtataattta agcgttctgc tgcttcaaaa attttttgct
     4381 ttgtggctaa gccaacggaa agatcaggat catagttgag aactcttgaa acagtagcag
     4441 gagaaactcc tgccaactgc gcaatgtctt taattgttgc catttgtctc tcctcctttt
     4501 ttacgtttat aaaatcgcag taaatcgttc aaacgctgcc tgtccttttt catctcgctt
     4561 gaatactcca gcgtcttcca gtacacgtgc aaaaacctga cctacagatt tttgtacgac
     4621 atcggtgacc gtatctttcg tcaaatcagg atgttctttt tttaattgtt ctgcccacgg
     4681 acgatgatat tcagccatct ggttttcttc tcctaataag aaatgctcta cttctactag
     4741 ttctggtttc aaacgaggtg gtaaaacagc aagccccatt acttcgatca acccaatgtt
     4801 ctcctttttg atatgttgga catctttatg tggatgaaaa atcccatcag gatgttcttc
     4861 tgaaacattg ttgtctcgaa ggacaaggtc caactcaaag taattgtccc ttcttcgtgc
     4921 aattggcgta atcgtatggt gtggtgtacc atctgctgaa aaagctctga tagatactgt
     4981 ttcatccgaa tatgctttcc atttttccaa tactgagtta gctgcttcaa taagcaagtt
     5041 tttctctgtt gattgcaaac ggatcacaga catcggccat ttcacgactc ctgcgatgac
     5101 ttgcggataa tcttctagtt tgataagttt ttctatcggt gcttttgcca taggaaattc
     5161 atgacgtcct gcctgataat gatcatgaga aagaatcgat cctccaacaa ttggtaagtc
     5221 agcattcgaa ccaacaaagt aatgaggcaa gacttcagta atccgaagca gtctagcaaa
     5281 tgtttccttt gtgattttca tcgggcggtg ttgttctgat aaaatgatcg aatgttcatt
     5341 gtaataagca taaggggaat attggaatcc ccagctttca ccgtctagat tcattcgaat
     5401 aatccggtga ttggtgcggg ctggatagtt caagcgacct ctatatcctt cgttttccat
     5461 gcaaagcatg caggtaggat agtctacttt taccgcctcg cgttctgctg cgatttgttt
     5521 tggatctttt tctggcttag ataaattgat tgtgatttcc agttcaccat actccgactc
     5581 tgccggaaat ttgatgtttt ttgcgatggc tcttgttttg atgtagtcat ttttcttact
     5641 taaattaaag aaatactcag ttgcttcctc tgggcttttc gaatagtgct gtgcaaagaa
     5701 tgcattgacg actgaaggtg gcggtgtgag aaaatccatt aattgcgctt caaaaatctc
     5761 ttgttctgct ggcaattctg aaagaacacc attttctctt gccttagcga caagctgatc
     5821 caataagtct acagattctt ccttaactgg tcttacttca atgctttcta gagcatcttc
     5881 tcctaccatc cctaaaagac gattctgtaa atataagcgg tccatttcca tccatccgcc
     5941 agcttcgatc gcaagtgtgg caaaatcaca aatcgtttga ctgattgaca tatctcattc
     6001 ccctattctt cataaccatt tggatggctg acatgccaat cccatgctgt tttgataatc
     6061 gctttgatat ctgtgtattc aggtttccag cccagaatct ctctcgcttt ttcacttgaa
     6121 gcgactaatc ggctaggatc tccagctctt cttggtgcta tttctgctgg aatttctttt
     6181 cctgtcactt ctctagctgc ttctagcatt tccttgactg agtatccttt gctgctgcct
     6241 aaattgaaga agttgctttc atttccttct ttcaagtatt ccaaagctaa aatatgtgca
     6301 gcaatcaaat cttctacttg aacataatct ctgatacatg ttccatctgg tgtatcataa
     6361 tcgtctccat aaacagccaa ggcttttctt tgaccaagag ctacttgtaa aatgattggg
     6421 acaagatgcg tttctggtgt atgatcttct ccgatagatg catctgcttt tgctccggct
     6481 acattgaagt agcgcaaagc gacatagcgc atgccatatg cttggtcaca ccacttcatc
     6541 attttttcca tcatcaattt actttcacca tacggatttt taggattagc tggtgtatct
     6601 tctgtgattg gagactcttt cggctctcca taagttgctg cagtagaaga gaatacgatt
     6661 ttattcacgt tgaactcgtg catgacttca agtaagattt gcataccgta tacgttgttg
     6721 ttgaaataca tcaatggttt ttcgacagat tctcctacta aagagcttgc agcaaaatgg
     6781 attactcctt caatcggttc cttttcaaat acggaacgta gaaattcttt atcccggatg
     6841 tctccttcat aaaaatgagc atctggatgt actgcttgtt tgtgacctgt gagcaaatta
     6901 tctaccacag ctacttgata ccccttttga actagctggt ctacagcgtg cgaaccgata
     6961 taaccggctc cgcctagtac taaaattgtc ataaaaaaca ctcctttgat tagtaaaatt
     7021 gtgtttgttg attctatata atttaaaaaa cgaagtttac ttttatggtg aaaac
//
LOCUS       DAJSMI010000135         7069 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJSMI010000135 DAJSMI010000000
VERSION     DAJSMI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.137"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            177..404
                     /locus_tag="ON430_002226"
     CDS             177..404
                     /locus_tag="ON430_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR3220130.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            420..1586
                     /locus_tag="ON430_002227"
     CDS             420..1586
                     /locus_tag="ON430_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR3220131.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1821..2864
                     /gene="hrcA"
                     /locus_tag="ON430_002228"
     CDS             1821..2864
                     /gene="hrcA"
                     /locus_tag="ON430_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCR3220132.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2958..3521
                     /gene="grpE"
                     /locus_tag="ON430_002229"
     CDS             2958..3521
                     /gene="grpE"
                     /locus_tag="ON430_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR3220133.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3572..5401
                     /gene="dnaK"
                     /locus_tag="ON430_002230"
     CDS             3572..5401
                     /gene="dnaK"
                     /locus_tag="ON430_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR3220134.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5552..6718
                     /gene="dnaJ"
                     /locus_tag="ON430_002231"
     CDS             5552..6718
                     /gene="dnaJ"
                     /locus_tag="ON430_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR3220135.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2412 a         1212 c         1594 g         1851 t
ORIGIN      
        1 ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg taaaaaaatc
       61 atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac gtttagtatg
      121 cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag gaatctgtga
      181 aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc tttgcgatta
      241 ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag caagcatttt
      301 tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg gtgcttgaac
      361 gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa agaatttcaa
      421 tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat atcccatttt
      481 gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg cagccagtag
      541 atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt catcctacag
      601 cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct gctaaacaac
      661 ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt aatgagttta
      721 ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg aaaaactatg
      781 gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg aaaaaaattg
      841 gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa gcagaaaatt
      901 ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta gaaggatatc
      961 gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg tactcattga
     1021 tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc gaactgcctg
     1081 atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa aagcatggac
     1141 gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa cataacctca
     1201 tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt tatcttggag
     1261 atattcgtta ccgcaacaaa ggaccgatcc aacactattt acgcccatta cgagcaaatg
     1321 aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt
     1381 tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat cgctatcaaa
     1441 aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa gggttacttg
     1501 ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg aacaatgtgt
     1561 ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc gaaacttgaa
     1621 aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa ctggatttac
     1681 atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga gtctttctct
     1741 tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga atatatgagt
     1801 gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta cgtctgatca
     1861 tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg tctgagggag
     1921 tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat gaaggattgc
     1981 tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat cgttattacg
     2041 tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg attcgtcagt
     2101 ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct gagatattat
     2161 ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac cgccgactga
     2221 ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc gtaacagata
     2281 tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt caggaactag
     2341 aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg acggtgtacc
     2401 ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg acagaaggga
     2461 tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt tatgtcagtg
     2521 gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc aaatcgatgt
     2581 tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg gatagcaata
     2641 ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg agtatgattc
     2701 aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta gggcctacta
     2761 gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa ctagcagcta
     2821 aacttgctga ttattaccgc tcgcttgact tgtcaaactc gtaatgtgaa taacgaagct
     2881 atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg actgaataat
     2941 gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa atgacagatg
     3001 ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt tcagaagctg
     3061 aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg gaagatagat
     3121 acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat gagcgtgagc
     3181 aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca attgacaacc
     3241 tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg aaaaaaggcg
     3301 tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt gaaaaaatcc
     3361 ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact gttccagcga
     3421 ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac aaattacatg
     3481 atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga tataaaactt
     3541 acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta ttgacttagg
     3601 tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca tcgctaaccc
     3661 agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa tccaagtagg
     3721 ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa tcaaacgcca
     3781 tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac cacaagaaat
     3841 ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag gtgaaaaagt
     3901 cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc aagcaacaaa
     3961 agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac caactgcagc
     4021 tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag tattcgacct
     4081 tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct ttgatgtatt
     4141 gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa tcatcgacta
     4201 catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca aaatggcttt
     4261 acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg tatcaagcac
     4321 acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt tggaaatgac
     4381 attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta caaaagtacc
     4441 agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg aagttatttt
     4501 agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag aaacaggaaa
     4561 agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg ctatccaagg
     4621 tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac cattgtcatt
     4681 aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata caacgattcc
     4741 aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag tagacatcca
     4801 tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa gattccaatt
     4861 gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat tcgatattga
     4921 taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag aacaaaaaat
     4981 cacgatcaaa tcttcttcag gtctaacaga tgaagaaatc gaacgtatgg tgaaagatgc
     5041 agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac gtaatgatgt
     5101 agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca aagtggacga
     5161 agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg ttgaagcaaa
     5221 caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc aaaacttgac
     5281 tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag aagcagctca
     5341 aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg acgataaata
     5401 atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt tcttttgaaa
     5461 aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac aagcgacgtg
     5521 aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt attatgaagt
     5581 cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc gtaagctttc
     5641 gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat tcaaagaagt
     5701 atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg accaatacgg
     5761 gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat ttggtggatt
     5821 cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact ctttctttgg
     5881 aggaggcggt cgatcagtcg atccaaatgc gcctcgtcaa ggtgctgatt tgcaatatac
     5941 ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca agtataatcg
     6001 ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac aaccaactac
     6061 ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc cacttggacg
     6121 agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa tcaaagaacc
     6181 ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta aagtgaatgt
     6241 accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag aagctggaga
     6301 aaacggcgga ccatttggtg atttgtatgt ggtctttcgt gtagaagaaa gtgatatctt
     6361 tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac aagcagcttt
     6421 gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga tccctgctgg
     6481 aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt tacgtggtgg
     6541 tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc taaatgaaca
     6601 acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg aagaacagca
     6661 agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc ggaagtagtt
     6721 tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta aaaacgctaa
     6781 gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctggtttt tttgtagttg
     6841 tcatgtttgt accaacaaaa aagccagctt tagaataaga taatcatcat agtttctgaa
     6901 ggtggtatcg actatgtact tgatatcgtc aatgaaccga tggtactttc ttatgcgatg
     6961 gaacaggtag agcgattgct tgtttgtgca ggagaacaag cgatgcggct ggtacagtta
     7021 agtgaaaaaa caagaaacta aaaatgcccg gtcgtcatga agcaaccgg
//
LOCUS       DAJSMI010000136         7048 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJSMI010000136 DAJSMI010000000
VERSION     DAJSMI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.138"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            10..726
                     /locus_tag="ON430_002232"
     CDS             10..726
                     /locus_tag="ON430_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCR3220136.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(786..2804)
                     /locus_tag="ON430_002233"
     CDS             complement(786..2804)
                     /locus_tag="ON430_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR3220137.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2820..3816)
                     /locus_tag="ON430_002234"
                     /pseudo
     CDS             complement(2820..3816)
                     /locus_tag="ON430_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            4014..5255
                     /gene="sstT"
                     /locus_tag="ON430_002235"
     CDS             4014..5255
                     /gene="sstT"
                     /locus_tag="ON430_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR3220138.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5402..6037
                     /locus_tag="ON430_002236"
     CDS             5402..6037
                     /locus_tag="ON430_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR3220139.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            6228..6968
                     /gene="truA"
                     /locus_tag="ON430_002237"
     CDS             6228..6968
                     /gene="truA"
                     /locus_tag="ON430_002237"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR3220140.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
BASE COUNT         2256 a         1155 c         1381 g         2256 t
ORIGIN      
        1 aacggcgaat tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt
       61 tgtaccaacg ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt
      121 gcaactacta caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca
      181 gatacctcta cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca
      241 agttcttctg tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca
      301 ctgtggaatg cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca
      361 atgggacaac aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa
      421 gtccctcaag ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct
      481 gcagaatggt cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc
      541 gatgcagaga cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat
      601 cagccaaaag tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc
      661 aacgagacag cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca
      721 aattaatgag ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt
      781 atttgttagt cttttatttt ttgtgtcgcc catgttgcac tgtactggtt acctgcaaca
      841 agatcatatt gtatcagacg ataatcttct agaatttctt tttgtttctc agttaaattt
      901 ttttcttgga taatttgact atcctctgta acaaagacct gttttccatt aaaacgatta
      961 gacaagcttt ctgtaggatc aattgttaat gcaggtagat gattagatac ttctgtcaac
     1021 aacgcatagt aaggagttac tttgatattc gcatgctcaa gagccatagc accaaagtta
     1081 ttaggagcaa caacttcttt atttaacgta gctgcttgtt cacgactata cttgttgtta
     1141 taaatgaaat aatctgtctg gtgctgttct aatccatatt ttttcgaatc attcccacta
     1201 tagattgcgg gtaaatggtc accataaaaa acaaaggtaa tcggtttatc gattttgtcg
     1261 atttcttgga tgaatttttt tactgctgta tcggtatagt gaaggccttg catataagtt
     1321 tgtaattctt ttcttctatt aggaatgaca gcagttcctt cagcagtgta gttcaattca
     1381 ctatagtaat catcataagg catatggttt tgcatagtgg ataattggat gaattgtgac
     1441 gtttctgtat tgctttgtaa agctttcaag gtttctttgt atgctgaatc atccgatatg
     1501 cgtgggcttt gttctatttt atccgtataa gttaatttat ccggactgtc taaataatag
     1561 aacctgtcaa aaccaaattt atcaaacact tgttttctgc gataaagagc agctgtaaaa
     1621 ggatggatgg ctattttttc atcgaataga tcggtaatat ttggacttat tttctgacgc
     1681 tccactagct gggtataagg tgtaactaac gaagcagaca ggttactgat actcaagcct
     1741 gtcagtcctt cccattccat gttagctgtt ccgccaccat aaccgacgga taacatcaat
     1801 cctcctgttc cttctgttat aatgcttttg atgtaaggaa tagggtctgt aggtactgtc
     1861 agattaggga cacgtgcagg atcactgaag ctttcactta aattgagaac gatcgtctga
     1921 ttgtttaacc agtcacttct tgtttggttg atttcatctg cctttgtgtt gtactttttc
     1981 attattccat taattttttc tttcgaatag ccagcaggtt cttccatgat tttaatgtcg
     2041 atattgttta ggaattgaat gactggtccg ttatctttta cggcgccttg ctggttaaag
     2101 aaagtcttgt ttactttaaa taaattaaac agcaagaaag aaggcgaatt tttgtgatta
     2161 atgaaaaaga cacccgaaaa acatcctaat aatacagcga tactgataaa tcgacgtttc
     2221 caatgaatct tcaaggaata ctgtttttgt agtctacgtt gtacaataat actagtaatc
     2281 aataatagga gaaggatcaa tccgcccacg attaataaga ctgggctaac catcgataat
     2341 agttctgaca atccggtcag catttttaaa tctgaaggaa agacaggctc ttcccgcatt
     2401 tccattttga ttactgtcga aatagtgagc agtaagttaa tgatcaaggt agcagtcaag
     2461 gcgtaccaaa aacgattgga aatcaacaat agtaagaaga aaaatcccat aataatgatg
     2521 attgtcagga tcatgggtgc ttgtcgctga gtaaatattt ttaaggcaac ctggatagtt
     2581 tctttccaac cttcttttgg ttctaaaacg aaaatctgca cgaaggtaaa gcagtaaaaa
     2641 aatgctgcaa ctaaaaaata acttttatga acaaaaagcc aatgaattct ttttttccaa
     2701 cgctgagtta attgttcaat cgaatcaaaa gcgaagtatt cgatgaaacg atgaatgaaa
     2761 cggatttttt gtatataaaa taaaaagata actaggaggg acactgctaa taaatagcaa
     2821 ataaaaaact gcccgatatt tatcagccat gccttccctg agtttggaaa ttcacgtttc
     2881 ataaatgtag atacgcgata agaaactaaa gggaaattac atataacttg agtgacaatc
     2941 aaaaatacag caagaaaatt tcctcttcct tttataccta atttatcaga tagcatttct
     3001 aaaaataaaa acgaagagaa agtataatac attgcgaaga gagaatatgg tttagaaaac
     3061 ctcagcgctg tatctgtatt tcctctgatg atgagtgaaa tttgtgtcat caaaataatc
     3121 aaaccgaata aaacaatcaa gctgataaat acttgaggaa gcttatagcg gaccttttct
     3181 ttccagttcc attctttcaa accataaccc attaaaaata aataaaaaat ccataaaata
     3241 ctctgtccat tctgtaatgc aaacgggtct ttattaaata gagtagagca gccaactaat
     3301 ccaattgtta aaaataatag tagatattta attgttgttt tcgataattt atttaaaaat
     3361 ccaactgtat aaggaatact acaaaaaagc aaagtacaag aaacagcata agtaaagtaa
     3421 ttctgactga taggtgttag gataagccaa taatcacgaa tattcaagct gccagcttgt
     3481 acgtaaaaaa aagatattgc aaactagtgt ggctattagg aatataaagg caaaacgact
     3541 taaataatgt aaaactttta aacgggcgat tttttgatat tggtaaccca tgtaaagtgg
     3601 gatcaaattt gcaccaataa caacggaagt ttgtaaaaat gtatagatgt agaatcgacc
     3661 agggtttgtt gcatatgtag gaatagtgac tgctgaaatc aatatacgct gtaaaaccat
     3721 cattatacaa gcgacaaaga ttgtcaggaa gccataactc tccttcttta tttttttcaa
     3781 gaataaacac ctcacaaaaa aattggacaa aaacataaga aaacgtttcc tgataagttg
     3841 cccattaata acatcctaac ttagaatatc tcaaaaaaca acgtgtagac agaaaaagta
     3901 caaaattagc aagaaatggt tgtttgtatt gttagaattt ttagaaaatt atacctttag
     3961 tttctttgca taaaatgata caatgattgt taaaattgtg aaagggtggt catttgttag
     4021 agaagttttt ggatatgtcg ttagttcaac gtattgttgt tggtatttta gtcggaatat
     4081 tgtttggtat ctttttcccc acatggacat ttatttcaat attgggaacg ttgtttgtca
     4141 gttgcttgaa ggcaatcgca cctattctgg tttttttctt gacgatggcg tcaattgcca
     4201 agcataaaat tgggaataag acattcgtga agcctatttt gatcttatat ttagtgggaa
     4261 cattgctttc tgcacttgta gcagttattg taagttcaat ttttactgtt cctattactt
     4321 tacaagaaac agtatctgaa aaagcaccgc aaagtttgga aagtgtttta tctactatgt
     4381 tgacgaatgt cacacaaaat cctgttcaat ctcttattga tgcgaattac cttggcgtct
     4441 tattctgggc tgtattgttg ggactagctt ttcgagcacg ttcagaatcg acaaaagagt
     4501 tggtggatca gatatctata gcgttgtcac aagtcgtaca agtgattatt gcatttgctc
     4561 cgatagggat tttaggattg gtttatcaat caattgctac gactggaatc gctggattgg
     4621 cagagtatct gcagctttta cttgttttga tcggtacgat gctgtttgtc gcattagttg
     4681 tgtacccatt cctcacgttt ttgtttatta aagaaaatcc ctatccattg attttctttt
     4741 gtttgaaaga aagcgcactt cccgctttct ttacccgaag ttctgccgca aatatcccga
     4801 taaacatgat gttggctgaa agattgaaat tgacaaaaga gtcttattcc atatcgatcc
     4861 cgttaggtgc tacgatcaat atgggcggtg ccgcgattac catttccttg atgacgttga
     4921 ctgctgtaca ttctttaggg attgaagcac cttttgtttt gaaagtcctt ttatgtattt
     4981 tgtctgcttt agctgcttgt ggagcttcag gtgttgcggg cggttcactg ttattgattc
     5041 cattagcatg tagtttgttt ggtatctcta atgatgtcgc gatgcaaatc gtcggtattg
     5101 gatttattat cggtgttatc caggattcaa tggaaactgc cttaaattcg tcaagtgatc
     5161 tgttgtttac tgcaatagga gagctaggcg cgcgtaaaag aagtggggaa aaaatcaaat
     5221 tgaaagattg tttagatgga ttaagtgaac gttgaacgtt agatatttag tatttttaaa
     5281 atgattcgag tttttaatga gttatataga tgttcgtgta aatagaggtt tctgttaaat
     5341 caagataaag tcgtttataa taacaattaa aagcataggt aattgttatt ggaggcgtat
     5401 catggatttt ttacgtaaat ttggtttttt ctttttatta ttaggcgtgc tgagtgattt
     5461 tttgactccg tacgtattag gattgtatta tcctgaatta gatcaaatga agagcgtgat
     5521 cagtgtattt ggagaggttt ccagtcctgt aagaaaagcg tttcttattt ggtctgtggt
     5581 ttcagggatc tttttcatcc tgtcattgcc tgcattgtat gctgcatttg taagtacatc
     5641 tcgtgtgtta gctaccctag ttacagcagc catcgcgttc tatggtatag gtgattgtat
     5701 ctttacaggt ttgttcagta tcaataccca tgaatcttcg tggaatgtct cgacatgggt
     5761 ccataatatc ggatcaggtt taggttacag tggtttttta ttgtttccag ttcttctttt
     5821 cttgatctat cgaaaaaatg ggcaaatgga cgtaagccac ttataccttg gactgtctgt
     5881 gatcagtttg ctttttgcgg cgatttatgg actggcaaga attccttcta taaattcttg
     5941 gccattactg aataaaatag gtttttgcca aagaatcagt tttttcttta attatgtgcc
     6001 cattgcctgg ttgagtatct tgcatctcaa acagtaaaac ttgagccgtc aaattgaaca
     6061 gtcagcggat acttcatccg gtggctgttt tttgtttttc tgaaatatat atttccacag
     6121 ttttcttgta tagcttctgt tttagagaag aaaggagcta tattttgtgc tacaatctta
     6181 aaaaggtaga gagaagacca aatggaagac agaggagcca ctaaaatatg agaaacatca
     6241 agttgacaat cgaatatgac ggaaaaagat attcaggatg gcaaagactt ggagatgacg
     6301 ataaaaccat tcaaggaaaa atcgaaaaag tattgcatca gatgacaaat gaagagatag
     6361 aaattattgg atcaggaaga acagatgctg gcacccatgc gcgaggacaa gtagcaaatt
     6421 ttaaaacaaa cacagaaatt ggactggcag aaatgatcga ctttatgaat cgttaccttc
     6481 cgcgggatat tgtcatcaaa agaatcgaag aaatgcctga gcgcttccat tcaagatata
     6541 atgcggtggg caaaaaatac agctattatg tttggaacaa cgtgattcct tcagcatttg
     6601 agcgaaacca cagcttttat ttccctcaag agttggatat ggacaagctg aatgcagctt
     6661 gcgaaaaatt agtgggtaca catgatttca ttggtttttc tgcattgaaa aaatcaaaaa
     6721 aatccacagt gcgtacgatt gatgaattat ctatcgaacg aaagggagaa atgcttcatt
     6781 ttacttttgt cggagacgga ttcttatata aaatggtgcg gattatcatg gggacattac
     6841 tagaaatcgg cacaggtgcc atgccgatag aaacaatcga tgaaatattt gaaagcaaag
     6901 tgcggcaaca tgcaggagaa accgtccctg cacaaggtct gtttttggat gaagtttatt
     6961 atcgataaaa atactttgat aatggatgaa acgcatgttt cacagtataa atatcgatgt
     7021 taaataatgt atgaagcaaa ttaaagaa
//
LOCUS       DAJSMI010000137         7001 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJSMI010000137 DAJSMI010000000
VERSION     DAJSMI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.139"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..304)
                     /locus_tag="ON430_002238"
     CDS             complement(<1..304)
                     /locus_tag="ON430_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR3220141.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLK"
     gene            complement(445..1047)
                     /locus_tag="ON430_002239"
     CDS             complement(445..1047)
                     /locus_tag="ON430_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR3220142.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            1292..1498
                     /locus_tag="ON430_002240"
     CDS             1292..1498
                     /locus_tag="ON430_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220143.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            1810..2529
                     /locus_tag="ON430_002241"
     CDS             1810..2529
                     /locus_tag="ON430_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3220144.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(2610..3293)
                     /locus_tag="ON430_002242"
     CDS             complement(2610..3293)
                     /locus_tag="ON430_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCR3220145.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            3511..5697
                     /locus_tag="ON430_002243"
     CDS             3511..5697
                     /locus_tag="ON430_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR3220146.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            6043..6453
                     /gene="spx"
                     /locus_tag="ON430_002244"
     CDS             6043..6453
                     /gene="spx"
                     /locus_tag="ON430_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR3220147.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            6467..6823
                     /locus_tag="ON430_002245"
     CDS             6467..6823
                     /locus_tag="ON430_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR3220148.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         2594 a         1150 c         1328 g         1929 t
ORIGIN      
        1 atttcaaaac aaaaaaatcg ccgatgcttg ttaagtgatc tcctaaaagc gacgtcaata
       61 acccaaaagg aagcgcgtaa accaattgtt gacgcgaagt cgaaaagaag aaaaaagtaa
      121 gcatcagcat tcctatagca attagagcag taaaaatccc taaaatcgaa tagacccatc
      181 caatcaaaat acaagaaatc agaatgactc ccgcaaacag atatttgtat cggatgtgat
      241 ggtcaatcaa taagatacaa attgtgtaaa tcaaatattg caacgctatg gaccataaaa
      301 acataaagta cagctcccat tgatttttac caaattatag cacaattggc gtattatttt
      361 tttaattttt cagggatttt gaattcagaa ctcataccaa agcagcagcc agatacaaaa
      421 aagtcagata agttagttaa ttgtttaatg attgtttttt tcatgaaaga ttcctccaaa
      481 attcatcaag tagtttgtaa taggaagcaa cgtagctatt tctaaaacaa tcccaagtag
      541 caaataggta gaaaagtaag aagctgagaa aaataacgta atgagagaaa aaattagaaa
      601 catgctgatc gccttcttta ataatggggg acgtttctcg ggttcaatgg gattgattgc
      661 tgtacctctt gatccatagc gaagcagtaa caaaaagcag ctgattaaca aaaagaccat
      721 cattgggaag gagatagtca attgcagatg tttcatcacc aagggaacgc cccataaata
      781 aagtagtgtc cacagcagac aaacgaattc agagtcggaa tgcaatccat aagcatatct
      841 gcggatgcag atataagaaa gaaaggtgat tcctgtttcc gcccatgagc ctgtcaccag
      901 agaaaagact agaacgaccg ctagttttcc aagattattc aataaaactt ctaagacaaa
      961 ttttacttgg acatacacca tatcttcttc cgatttgtct ccgctgaata aggcttctaa
     1021 taatttgttc acaactttat ctttcatctc atacgtacct ccaccacaaa ttttatgcta
     1081 aaaaaaataa aaaacgtctt ttgtttcata aaaagtggtt ttgacatcaa aaccgtcatg
     1141 atctcctaaa aaaatcttcg ttttccaaga accttaccaa aaaaagtgac agttttgata
     1201 cgaaaagtac tttttatgac aagtttgtgc tttttcgagt gatttatgtt aaaaaattac
     1261 tgaccttata aaaggtgatg gagggattct tatggaaaat gaacaattgt ttgaagtcgg
     1321 cgaaacatat gcatttaaat ctgatttatt tgaatcacca ataaaaggaa aaatagaaaa
     1381 aatctatgaa aatagtgtga tgatacgtgt catgaaatgc gcagtagaag acaatgatac
     1441 cgctcataat ctgcactggc agttagttgt acgcaaatca agtacaatag aagaataaaa
     1501 acagataaaa tgttgagagc ataaagaggt tgtgagtctc ccgtttagct ctgagttaca
     1561 aggaataatg ttaaaaaaca gcttttcatg ttttatgaat attgccttgt gaccgaggtg
     1621 ttggcaggag aatactgttt atttgaaaaa agggctgcga caagactttt gtcacagcct
     1681 ttattttcat gaataggagt tacttttggc tacttttaga gaagtagctt ttaaggagca
     1741 gataaagtca aggcttttat ttctataaat gaagtagtaa aatgaagaac agattttatt
     1801 acaggaagga tgccgataat tgtgatcatt gttgcaggaa cgatcggtgc agggaaaagc
     1861 acactaactg aaatgcttgc ccaagattta gaaacaaaac ctttttatga gaacgtggaa
     1921 gataatgaag tattgccgct attttattct aatccagaaa agtatacttt tttattacag
     1981 attttctttt tgaacaaacg ctttttggca atcaaagatg ctttcagcca tgatgacaac
     2041 gtactagacc gctccatcta cgaagatagt atgctgtttc atttgaatgc agatttaggg
     2101 cgtgtttctg aagtagaagt aaagcagtat gaaggtcttt tagaaacgat gctgaaggag
     2161 ttagaagaaa tttcgccaca aaagaaacca gatctactgg tttatattcg tgtatccttt
     2221 gaaactatgt tagcgagaat caaaaaaaga ggccgggaat atgaacagct agaacaagat
     2281 cctgaactct attcctacta taaagaactc aatcgacgat acgaagagtg gtatgaacag
     2341 tttgatatat gtcccaaaat agtgatcgat ggggacaaat atgatttcgt agccgatccg
     2401 gcatgtggag aacaaatcgt acaagaaatc aagatgcggg caaagaaaat gacagaagag
     2461 gcagatgccc agtcgatccg ctacactaca aataacccga aagcaaaggg aatctttaca
     2521 actaaataat ataaaaacta agcaaattgc tcactcgcag atatttctct gtgagcagca
     2581 tcttgcttag tttttttgat tcattgagat caaatagtga accgattcgt atgataaaat
     2641 tgccataaat gaaatgtctg agtgagggcc atgatcaaaa ataaaatgga aacgatcaaa
     2701 atcgaatggg cgccaaacag atgatgacag aaaacaagga caatagctcc tgcgataaag
     2761 gcaagaacga cacttagata gtggaaaaat cgatcaaaat ctgctttttc tttagaaata
     2821 caataatcat ataagttcgt tgctacccct tttaaatttc cagttgtaaa taaattagtg
     2881 taggcacgcc cgttgatttt atcaaaagct acccactgga tcgatgcagt aaaagcaatc
     2941 aaaatcgtta ctaatgcggg atgctcatgg acgggtgtca aactaacaaa gaaaaaaatc
     3001 actgcttcat aatatagaac aaacaagcgc cagaaatgtt tttctttttt tctaaaataa
     3061 tcaattagat actttgttag aagaatccct aagaaaaaga aaagggtaga caatactttt
     3121 ttaccaacac ttagccattc tccgtcaaat gcttggatga tcgatagaat gatattgcca
     3181 gtttgagcag aggcaaaagc accatattgg atataagtat aactgtccat ggctcctccg
     3241 ataaatgtca gcaaaagtcc tattgtacgc ccctcgtgaa aggaagtctg catttttttc
     3301 atcacctttt atgaatattt ttgtcttcct tatcttagca taaacgattg aaaaaaagga
     3361 aaaattttca gaaaactaaa atcatatcgc ttcccatttt catttttaga aaggtctgtt
     3421 agtataaaga aaaaatagac aacgaaactt taaataacca acttgaaata aacaaattca
     3481 aagaagaaag atgggaagga aatcaatcag ttgaatgaaa aagaaattct ccgattacaa
     3541 aaagaattga aacgctggga gaacaagtat aaaaagcttc aacaagtctt tcggctacaa
     3601 gaagaaatca ttgctaccta tgaagcggct tatggcgaag cacccggact tattgaggaa
     3661 gaagtacaag taaaagaaag tgaaggaaac agctctttct ttaaaatgat ggagaaacgt
     3721 tcctataatc gcttcaatca agaacaaaaa gcagctatca catatgatat gaagaaacat
     3781 cttcgaatca tagcaggagc tgggagcggc aaaacgcaaa caatctgtgc aaaagctgtt
     3841 tatctgatga cgcaaaaaca agtagacgaa gaacgcatac taatgatcac ttttacaagg
     3901 aacgctgcaa atgaactaaa aaaacgagta gataatttca gccagagaaa aaccgctgtc
     3961 catatcggaa cattccatag tatctttttt aggatataca atgaaatttg tcgaaaattt
     4021 cctgaagtag caatgcaagg aatacaagga gacttttcaa aggatactgc acaaaaagtg
     4081 aatgctgttt tgcaacagct gatacgtaaa tataatcttt atatatttga tcaatatgga
     4141 gaaaaaacga ttgcttctcg gttagactat tggcagaata tgaacctttc agtagaagaa
     4201 atggttcagt tagttaaaga aaaatatgat tccattgata agaatgcacg acagccgata
     4261 tcagaacgac tgtatggatt gctcacggaa ctccaagaac aaaaaagaag ccagcaattg
     4321 ctagagttca atgatgtact ccaaaatctg aaaagcgctt tggagaatga ggagatccag
     4381 cattatatcg gccaaaaata tgattatcta ttcattgatg aatttcaaga tactaatcca
     4441 cttcaatggg aaatcgtgaa acggatgaca aaggaaaaca ggatcaagtt gattgttgtt
     4501 ggagatgatg atcagagtat ctatggattc agaggatcag agccagcata catcaagaac
     4561 tttgaaaaag aatatcctac caaaactctt ttcttattaa ctaattatcg ttctcgggca
     4621 gctattgtcc aaggagcaaa tagattgatc agctataata aaaatgatcg gataccaaag
     4681 gcgatgattc cagcacaaaa agaagcaggt gttatggaag cctatttgtt ttctgatact
     4741 aaagcagaaa gtcagtggct aatcaaacaa attcagacat ttattcaaaa aaatgggaaa
     4801 tataaggaat caatcatatt ataccgatca gcttcacaaa cgcagcagct tgtccaatct
     4861 ctgctgaaaa cgaatattcc ttttgtactg gaagcagata gtccatatga aggtatcttt
     4921 ggtatcaagt ctttccaaca tttctatcaa aaaattgttc attggcaaac agcactgaat
     4981 ctgcaaaaga aaaatcaggc ctaccagcaa ttgctccggc agctgatggc agactgctac
     5041 ctgaaaaaaa gtgaatgcga tcaatacttt tctcctaaat atcaaaatat agccgttgca
     5101 gattatattt tgtcgataag ccccaattta aaatcgaaat ctgcagaaat ccgagagttt
     5161 gaggaagcgt tgcgtaaaac agaccagcat caagaaatct attcgcttgt tcaaagctat
     5221 cttcatctgc cacgtatagc taaggaaatc gaccagagtg atcaagaatg gatacgagaa
     5281 gaagtaaagc agcatgctac tttcagaaga ctccaagcct tatcgcaaga aacccaagcg
     5341 acagctaaag aattaaaaac ccgcttgatt gcttacagac aaggaaaact tgatgcacta
     5401 tgtatccaga gcattcacaa aagtaaaggt cttagttacc gaaatgtctt tttgattggt
     5461 tgtaacgaag gaagtttgcc ttataacgga gcgacagaaa agaaagtaat cgactttaat
     5521 gaaataaaag cagaaccagt aacgacgata gaagaagaac gaagactatt ttatgttgcg
     5581 atgactagag caagaaatcg tctgtatcta tgtgtacctc aaatgaaaaa ttcaagaaaa
     5641 ttgaaagtat cgagatttat caaagaaacc gggagtcgtg tgaaaaaagg aaagtgatag
     5701 aagagagggt gtgacagaag tggtcagttt caagaaagaa gaaggaatgt acgaaaattg
     5761 cttctcaaat ttttgtgaaa tttcagctta ttttcgaaga agctatttct ggaacacagt
     5821 ttattcggaa aagggaggtg tgacaagaat tttgtcacac ctcccttatt tattttgaaa
     5881 atgagaatac tattatcaaa atagagaata tatagaaaaa ttctcattat tcaaacgtaa
     5941 ataatcagtt attttgattt tttttatatt ttattagcta ataattggta ttttaaaaga
     6001 aattactgta gaattggatt ataattaaaa aggagtgaga aaatgataaa agtatatact
     6061 tcgcctagct gtacttcctg tagaaaagca agagcatggt tgcaagcgaa tcaacttgaa
     6121 tttgaagaga agaatatttt tgccgaaccg ttaacagaaa gtgagatcaa aaagatcttg
     6181 gcagcgactg aaggaggagt tcaagatatt atttccactc ggtcgaaaat ttacgaaaag
     6241 ctgggcatag attttaacga attaacttta aagcaaatga tcgtcttatt caaagaatat
     6301 ccttcattat tgagacgacc tattttgatt gatgaaaaaa gaatactaat cggatttaat
     6361 gaagatgaga tccggtcatt tgtccctcgt gaaattcgat gtgttgccag aaatctgatc
     6421 tatttacaaa acaataacat cttaaattca tagggaggga gccttcgtgg aagtgaaagt
     6481 ctatggatca gcgcactgtg taacaagtct gaaggcgaaa caatggttag aacaccatca
     6541 attgccttat caatttgttg acttagaaga acgcgatcta agtacagaag aagcagaaga
     6601 attatgcagt tttgaagaag tgcaagcaga acagctattt gcgacttggt ctgaggcttt
     6661 tcacaacatt gcgattgacc tttcctgtgt aggaaaaaaa caactgattt ttttatgcac
     6721 acaacatacc aatctcctca gacgtccttt gattattatc gatgaagttc tatttattgg
     6781 atacaatgag cagttgatgg aacagcttat cttaaataaa tagactttaa ctgttttaat
     6841 caagtcactg agcatttcat acagttgtaa cagtcgaaca cttagcatca agcgtgttat
     6901 gtgttctttt tttgacttta tttttctact atagagacat gctcaattta tgtggtcaaa
     6961 attcaaatct aggtggggat atagagttaa aaatatattg a
//
LOCUS       DAJSMI010000138         6950 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJSMI010000138 DAJSMI010000000
VERSION     DAJSMI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.140"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            135..608
                     /locus_tag="ON430_002246"
     CDS             135..608
                     /locus_tag="ON430_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCR3220149.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            605..2737
                     /gene="feoB"
                     /locus_tag="ON430_002247"
     CDS             605..2737
                     /gene="feoB"
                     /locus_tag="ON430_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCR3220150.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2739..2873
                     /locus_tag="ON430_002248"
     CDS             2739..2873
                     /locus_tag="ON430_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCR3220151.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            2968..3612
                     /locus_tag="ON430_002249"
     CDS             2968..3612
                     /locus_tag="ON430_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3220152.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3799..4992
                     /locus_tag="ON430_002250"
     CDS             3799..4992
                     /locus_tag="ON430_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3220153.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            4985..6712
                     /locus_tag="ON430_002251"
     CDS             4985..6712
                     /locus_tag="ON430_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCR3220154.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2237 a         1225 c         1558 g         1930 t
ORIGIN      
        1 ataattgaca ttctcaatta ccgagagtat tctatgtgag aatgattctc atttatttgc
       61 gtaacacgta agaaaattag aacatataat gaatgatttt aatacatgat ttatttcaca
      121 agagaggaat caatatgcag accttagcgc aggcaaagca gaatttcgtt tatcaagtcg
      181 aacgaatcga aacagaagaa aaaacaaaac gccatttgca aaatatcggg atcgttgccg
      241 gaagtaaaat cgtcgtcgtc aatttatcag gcaacaatgc cattttactg gtaaaaagtc
      301 atcgagtggc agtaagtcgg gaaatactag gtcaaatctt tgtcaataaa aagactgctt
      361 cctctgaaag ctggacttct cttgatcaat taaaagcagg agagactggg atcgtcgtag
      421 caatccacgg acaaggcgca gtgaagcgac ggttaatgga tatgggattg acgcgaaatg
      481 taaaaatagt tatccgaaaa ctagcccctc tgggagatcc aatcgaactg acagtgcgag
      541 ggtatgaact gacgttacga aagtcagagg ccgaactaat cttgatacaa agggaggaaa
      601 aagaatgaat atcgcgttag tcggaaatcc aaacagcggg aaaactagct tgttcaatct
      661 cttgactggt tcgaatcaat atgtagggaa ttggccgggc gtaacagtag aaaaaaaaga
      721 aggatcgttg aaaaaaaatc cgaacattca tattcaggac ctacccggca tctattcttt
      781 gtctccttat acaccagaag aagtgattgc aagagattat ttggttcagg aacagcctga
      841 actgatcatc aacattattg atgctaccaa tttagaacga aatctttatt taactacgca
      901 gttattggat ttagacattc ctatgatcat tggtctgaat atgatggatg tcatcaaaaa
      961 agaaggaaaa aaaatcaatc tagaaaagct ttcctatggt cttggtgtac cagttttacc
     1021 aatcagcgta ttgaaaaagc aaggaatcga aaaactaatc gaaaagattc aggagtctgc
     1081 aaaaaagcgg ccttctattc ctgttccctg tacatatgat ccacgtttag aagcagcttt
     1141 acatgaaatt gaagatgtac taggagaaca aagcggcaag aaacgctggt atgcaatgaa
     1201 atattttgaa cgagatcaga aggtaagaga ggattacgag atagcagttt ctcagcaaaa
     1261 agagatcgag caactgatcc agttaacaga gaaactattg gacgacgata gtgagacgat
     1321 tctggtcaat gaacgttatg aatttattac ccaactatgt acactctgtg ttgtgtcaaa
     1381 tgattctttc caattatcga tgagtgacaa aatcgatcag attgcaacga atcgttggtt
     1441 agccttgccg atcttcgcat ttgtgatgtg gctgatctat tatctggcta ttcaaacagt
     1501 aggaacaatg gggacagatt ggatcaatga cacattgttt ggtacatggt taccagaaca
     1561 tgttggcaga ttgttagctg aatggcaagt agcgggatgg atgcaggagc tgatcttgaa
     1621 cggcatcatc gcaggcgtgg gagcagtgtt aggatttcta ccgcaattga ttatattgtt
     1681 tttatgcttg agctttctgg aagactgcgg atatatggcg aggatcgctt ttgtcatgga
     1741 tcgacttttc aggaaattcg gtctttcagg aaaatccttc attcctatgc tgattgctac
     1801 aggatgtgga gtgccaggcg tcatggccag tcgcacgatt gaaaacgagc aagatcgtcg
     1861 tttgacgatc atggtcacaa ccttcatgcc ttgttctgca aagttgccga ttattgcctt
     1921 agtagcaggg gccttttttc ctcatcaatc ttgggtagct ccttctgcct attttcttgg
     1981 tatgactgca attattttct ctggaatcag cttgaaaaaa acacgtttgt ttgctggaga
     2041 tacagctcct tttatcatgg aattgcccgc atatcatttt cctcaatggt cagcggtcct
     2101 aagacaaacc tatgaccgca gcaagtcttt tgttaaaaaa gcaggaacga ttatctttgt
     2161 ttctagtatc gtgatctggt tcagctcttc ttataatttt tatgctcaac ctgtttctga
     2221 agaccaaagt atcttagcct ttttcgggcg aatcctggca gttgtttttc agccgcttgg
     2281 ttggggaaac tggcaaggaa cagtcgcagc gattacagga ttggtcgcta aagaaaatat
     2341 tattggcaca tttggtattt tgtttggtca tgcggaagta tccgaaaatg gcagagaaat
     2401 ctggcaagtg ctacaacaca catatactcc cgcagcagct tattcattgt tagccttcaa
     2461 tttactttgt gcgccttgtt ttgcggcaat tggggctatc catcgggaaa tgaatgccaa
     2521 gaaatggact tggatcgcca taggctatca atgcggttta gcttatttag ttagttttgt
     2581 gatttatcag ttgggacact tattggaggg tggacaggta gcgttaggga cgtatcttgc
     2641 tattttactg atgataggac tcgtttatgt tttgttccgt cagccaaaat ccaagaacca
     2701 gttttatact atattgtcat tagaaggaga ggaataggat ggcgacattc ttattaagtg
     2761 tgttaatctt tggaagtgca ggcgcaattg tctatactcg tttaaaatcc ggtaagaatt
     2821 gtgatgactg tcaaacagct tgcccagtaa aaaaggaaca gatcaagcga tgatcagtcg
     2881 ttgcaagagg ctattctccc cttttttgct tattttgcta gagcatagta agatgaggga
     2941 aagtaaaaaa aagaaagagg aaagaaaatg tctggaaaga aaaaaatcgt ccgcccgcgc
     3001 taccagcaga ttgctgttga tttagccgaa cgtatcgttg aaaaccgata taaagtgggt
     3061 gagaagatcc atgcccgctc aacacttgcc agtacgtaca atgcttctcc tgagactacc
     3121 cgcaaggcta tcaatgtgct ggtagatctt ggaatcatgg aagtacggca tggaagtggg
     3181 gcatttgttg cttcgaagga aaaagcaaaa gatttcctat tgcaatatca agatgtacaa
     3241 tccatccaag atacaaaagc agaaatcatg gcaagtgtgg agaagcaaca agatgagctg
     3301 aatcattttt ctcaattatt ggatcagctg gtcaatcaaa cgaagcgaat acataagatg
     3361 aatcctttac ttccgtttga attatcttta tcagaaaaag caatgcatct ggaccgttca
     3421 attagcgagt tgaatatctg gcaggcgact ggcgccacag ttatcgcaat ttctcataat
     3481 gaagaactgc ttgtttcacc tggcccctat gctaaattta cggcagggga catcgttcat
     3541 tttattggaa atgaatatac actacagcgt atgacaaact ttttttaccc ggagcatcag
     3601 cttgcagaat gattgtgtga aatattaaaa aaatattaac ttggctaaac cttttattta
     3661 caaagattta gccaagtttt tttcagtttg gacaagcaaa tttctttcat ttttgacaaa
     3721 gtgactacat gatgttaaca tagtgtggtc acttttttca gaaagaaaaa tgaatttaca
     3781 taaaaaagga gaatttgttt ggtaaaagta gaagtaaaac atttaacaaa gatatttggt
     3841 aaaaagacac aagccgcttt agatatgatg aatgatcacc agccgaaaac ggagatttta
     3901 aagaaaaccg gtgcaacggt tggtgtgtat gacgtgaatt ttgacgtaaa agaaggagaa
     3961 atcttcgtga tcatggggct ttccgggagt gggaaatcca cattgatccg tctattgaat
     4021 cgtctgattg agccaacttc gggcagtatc tatattgatg gagaagatgt atcgaagtta
     4081 agcaaagaag aattgcgaga agtccgtcgt cataaaatca atatggtttt ccaaaacttc
     4141 ggtctgtttc cacatcgaac gatcttggaa aatacagaat acgggttaga agtccgtggt
     4201 gtacctaaag aagaacgaca agaaaaagca gaaaaagcat tagaaaattc tagtttgctt
     4261 tcatttaaag accaatatcc tagtcagtta tctggtggga tgcaacaacg ggttggccta
     4321 gccagagctt tagcaaatga tcctgagatc ttgctgatgg acgaagcgtt ctcggctctt
     4381 gatccgctga tccggcgaga aatgcaagac gaattactgg acctgcaagc caatgttcaa
     4441 aaaacgatta ttttcattac ccatgatttg aatgaagctt tacggattgg ggacagaatc
     4501 gccttgatga aagacggcga aatcatgcag atcggaacag gtgaagaaat attgaccaat
     4561 ccagcaaatg attatgtacg tgaatttgtg gaagaagtcg atcgttccaa agtgcttacg
     4621 gctcaaaaca tcatggtacc tgcattgacg acgaatatcg aatctgatgg accaaacgtt
     4681 gcattgacaa ggatgcgtaa tgaagaagtc agcatgctga tggctgttga cagaaagaga
     4741 cacctgaaag ggatcatcac tgcagatcaa gcgttagaag ctaggaagca aaaacgtcca
     4801 ttgatcgact tcctagatga aaatgtgacg gtgattggga aagacatggt ggtaagtgat
     4861 attttcaata ttatttatga ctcaccgact ccacttgcag ttgtagagga tggaaaatta
     4921 aaaggtgtcg tcatccgagg tagtgtgatt gaagcattag cagaaacaag cgaggtgagt
     4981 gaacatgaat aatctattga actatcaatt accagtggca aactgggtag aaaatatcac
     5041 agaatggttt acgacaactt tttctggcct attttcattt ttacaaacga ttggacaggc
     5101 agtgatgtcg gggattacaa atttactgtt agtgattcca gctcctttgt ttatcttgtt
     5161 attgacggta gcagctttct ttatttctaa gaaacgtcca ggacttacgt tgttcacatt
     5221 aatcggtcta tggtttattt ataaccaagg tctatggaat gatttgatga atactgtcac
     5281 acttgttctt ttatctagtg tgatctcgat catcattggg gtaccattag ggatcttgat
     5341 ggcaaaaagc agtaaagccc aaagtattat caaaccgatc cttgacttta tgcagacaat
     5401 gccaggattc gtttatttga ttccagcagt tgcatttttc ggtatcggga tggttccagg
     5461 ggtatttgcc tcagtcatct ttgctctgcc accaaccgtt cgttttacga atttagggat
     5521 tcgacaagtt cctaaagaat tagtagaagc ttctgattca ttcgggagca caagtcgtca
     5581 aaaactattc aaactagaat tgcctttagc taaaagtacg atcatggcag ggatcaatca
     5641 aacaacaatg ctgtcacttt caatggtcgt tattgcctct atgatcggtg cacccggctt
     5701 aggacgcggc gtattatcag cattgcaaag agcacaagtc gggaatggat ttgtcaacgg
     5761 ggttgcatta gttatcttag caatcatcat tgaccgtttt acacagcatt tgaaccagcc
     5821 aaacaataaa aaagctgcag gagcaacaac acagaaaagt aaaaaacgtc aaggactgat
     5881 cattggtgct gtagtagtag tgatcttagg tgcgattggt atagggagtt tttcatctgc
     5941 caaagaaaca aaacgtatca acttatctta tgttgaatgg gatacagaag ttgcttcaac
     6001 aaatgtagtc ggtgaagtat tgaaacaaat gggctatgat gtaacgatga cgccattaga
     6061 taattcaatc atgtggaaat ctgtttcaaa tggcgagtca gatgccatgg tttcagcttg
     6121 gctacctaaa actcatggtt cacaatatgc acaatataaa gatcaagtcg aagacctagg
     6181 tgccaatttg acaggagcaa aagttggttt agcagtgcca gcctacatgg atgtaaattc
     6241 gatcgatgag ttaaccgatc aggcaggtaa gaaaatcata ggaatcgagc caggagctgg
     6301 tgtagttacg gctgctgaaa acacgatcca aaaatatgac aacttgaaag actggaaagt
     6361 agagacatct tcttcaggag caatgaccgt tgcactaggt caagcgatca aaaaacacga
     6421 accaattgta gttacaggat ggacacctca ttggatgttt gccaaatatg atctgaaata
     6481 cttagaagat cctgaaaacg gaatgggaag cgaagagcag atccatacga tggttcgaaa
     6541 aggattgaaa gaagaccagc cagaagctta caaagtatta gacaatttcc attggtcaga
     6601 aaaagatatg gaaaaagtta tgttggaaat caacaatgga aaagatccac agcaagcagc
     6661 aaaagactgg atcaaagaga accaagaatt agtcgaaagc tggaaaaaat aaaaagacag
     6721 acacgaaata aatggagctt ccaaaattgg atcaaatatc caaatttgga agctccatgt
     6781 tcgtctctat cttttttgtc tataaaaaaa gagaatgcgt agctcatctt cgaaacgttc
     6841 aaagacgccg acaaactatt atgaatttat gggtgcgagt caaagagatc gatcgtaggt
     6901 atgaaagact aatgtgatta aagtttatta tgtacgaagt gcattctcaa
//
LOCUS       DAJSMI010000139         6857 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJSMI010000139 DAJSMI010000000
VERSION     DAJSMI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.141"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(132..575)
                     /locus_tag="ON430_002252"
     CDS             complement(132..575)
                     /locus_tag="ON430_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220155.1"
                     /translation="MEAYEFLGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMA
                     SIVGIGFAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMPRISRYSYDIY
                     LLHILVFSTIHQIIPNLVLAAFVSIPITLVLSYYYSIMLYRLKLK"
     gene            complement(663..1028)
                     /locus_tag="ON430_002253"
     CDS             complement(663..1028)
                     /locus_tag="ON430_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR3220156.1"
                     /translation="MYYFQQSYLPAILNKAISIGDTGVHIFFFCSGFGLYLSYLKKPI
                     TLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSHVLLFKIFLPVYEESL
                     GPFGLFRQLFSSISYSYQL"
     gene            complement(1372..3024)
                     /locus_tag="ON430_002254"
     CDS             complement(1372..3024)
                     /locus_tag="ON430_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCR3220157.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            complement(3084..3663)
                     /locus_tag="ON430_002255"
                     /pseudo
     CDS             complement(3084..3663)
                     /locus_tag="ON430_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            complement(4895..5611)
                     /locus_tag="ON430_002256"
     CDS             complement(4895..5611)
                     /locus_tag="ON430_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3220158.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
     gene            complement(5636..6034)
                     /locus_tag="ON430_002257"
     CDS             complement(5636..6034)
                     /locus_tag="ON430_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCR3220159.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            complement(6122..>6857)
                     /locus_tag="ON430_002258"
     CDS             complement(6122..>6857)
                     /locus_tag="ON430_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCR3220160.1"
                     /translation="YSDLTKKQWIDIFNRIRFVCSDFKRYSVSGDNIQKSLKDMYLDK
                     TFQRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQIEALLSVSVPRELFDITQFNIIHG
                     DLCFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYRYELAKLFHSVDGKYDFIIKDLFT
                     IKYDPKKAIIDYIVQDRKRDYDLYEVFYSVFKDEIGSDLKKIELIEALLFLSMIPLHG
                     ESLNHQMAMLATGLEILGRVVPDIYC"
BASE COUNT         2301 a         1487 c         1027 g         2042 t
ORIGIN      
        1 atcatttagt tttatctcct ttttaccaac catctaccac taggttacga actacctaag
       61 tgcccaccat ccgccgcgtg gaggctgtta atcaggatgg caaccgcccg gtagaggcga
      121 caattatgct atcattttaa tttaagtcta tataacataa tactataata atatgacaac
      181 accaacgtta taggaatgga aacaaatgcc gccaaaacca agttagggat aatttgatga
      241 atcgtactaa atacaagaat atggagtaga tatatatcat atgagtatct tgaaatccta
      301 ggcattatct ttctaaatgg aataacttga tacattaaaa ttgcaattgc accgtaaccg
      361 aagaatgcaa aaatatcatt aatagactcc atcagtccac caataatttt cgatatacca
      421 gcaaaaccaa ttccaacaat ggacgccata atcacgaata tattattaaa tcttactatt
      481 tttcttttca ctaaatactc tgcaaccaaa catgccgaaa gtaaattccc aaatatattg
      541 cgcaaagcag ctcccaagaa ctcgtatgct tccatatcct gtatgaaaca tcataatagc
      601 ccagatacta ataactcagc ttgcaacaag caatcatttc attcctattt ttctctatag
      661 ttttacaatt ggtatgaata agaaatagaa ctgaataatt gtcgaaataa accaaaggga
      721 cccagtgact cctcatatac tggtagaaaa attttaaata acaaaacatg agacaaatat
      781 acttttgcag catttaaatc ttgtgtcata taaggtagta acactgatac cgtaattacg
      841 ataatatacg gaatataaat cttgataaat cgtttctgta aaaattcacc caatgtaata
      901 ggctttttta aatatgacag atatagccca aaacctgagc aaaagaagaa tatatgcact
      961 cctgtatcac caatagatat tgctttgttc agtattgctg gcaaatacga ttgctgaaaa
     1021 tagtacatca atacaatagc gaatattgaa aacgccttct caaaactaat aatatcacgt
     1081 ttatttcttt ccccctcatt ttattatttc aatagtttta ttcatattct actcctttca
     1141 tctctattgc tgaatacatt attaaaactc taatatctct tatttctccc ttactgcttt
     1201 gatcggtgct tcatcaggct gctgtgtctc tgttaacact tctttgcctt ttcgatccac
     1261 acagtcggcc cctttacctc aattatgtaa acagaaccgt aaagcaggaa atttttatta
     1321 aacatagatg ttgctacttc ggacattgac actcagatct ttttcatagt attacttaaa
     1381 catttcagcc agttcagcag ccataacagc ttccattact tcggcagctt ccttgctttc
     1441 tgcgctaacg ctgatatagg tcttcagctt cggctcagtg ccggaaggcc gaaccactac
     1501 agagcagttg ccctccagca gatatttgag aacatcagac ttcggcaggc cattcagtcc
     1561 cgtgctgtaa tccaaggttt caatgacctt cttacctcca atcatatcaa gacctttatg
     1621 gaattcaacc ataattttct gcatcttggt aaaacctgcc gcaccctcaa actcaaagga
     1681 atgcagggtg ttcaaacagt agccatactt agcataaatc tcgttcagct tatcaaacag
     1741 actgataccc tgagtcttgt agtaagagaa catctcgcag atcagataag cggcgttgac
     1801 accatccttg tcacggacat agctgccgga tagatagccg taggattcct caaagccgaa
     1861 aatatagctg tccaccttgc ctgccttttc cagcatacca atctgttcgc cgatgaactt
     1921 gaagccggtc aacacatcga cagtacgcac gccatagttg gaagcgatcc gctcgcccag
     1981 atccatagtc acgatggtct tgaccatcac agggtcagtg ggcatcttgc cgtgcttaat
     2041 tctctgagag catatgtagt ccagaagcaa cagaccagtt tcgttaccgg acagcaggac
     2101 ataatcccct tctgcattct tcacggcgat acccacacgg tcgcagtcag ggtcggtagc
     2161 cagcagcaga tcagcattat acttcttggc atactccatg cccagagaca tagcttcatt
     2221 aatttccgga ttcggataag gacaggtcgg gaaattaccg tctggcagac gctgctcttc
     2281 gacaacggtc acattgctgt agcccatttc tgccagagta cgggtaacag gcaccagacc
     2341 ggtaccattc aaaggagaat acacgatagc aacattcttg tccacttctt cgccaaacag
     2401 aaccgactgg ccctttactt cttcaatgaa agcagtcaga acctctggct ggatagaagc
     2461 gattctggaa gcgtcgccgc ccattttcac atctgcaaaa atatccagct tttcaatttc
     2521 agccagaatc tcagcggcag cctcggtggt gatctggcag ccgcctgcgc catagacctt
     2581 atatccgtta tacttggaag gattatggga tgcggtgatc ataacaccgg cagaagcctt
     2641 cagataacgg atagcaaagg atacggtagg cacaggcagc agctccggcc acagatgcac
     2701 ctgaataccg tttgcagcaa agatgtccgc ggcaaccttt gcaaatacat cggacttgat
     2761 tcgggaatca tagcccacga cgacagaagg ggtttcatag gtcttattca gatagtctga
     2821 cagtccctgg cttgccttac ctacggtgtg tacgttcata cgattagtgc ctgcaccaat
     2881 ggtgccacgc agaccaccgg taccaaagga cagattttta tagaaggagt cctcaatgtc
     2941 cttctcggaa tatgaccgca gttcctctac aatttctgca ttggcatttg taagccaatg
     3001 ataataattt gcattatgat ccatagaatc tctccaatct tttaaaattc tgctcattta
     3061 tttttaaggt taggatgaat atatcagctc aaataatata taaccccact aatgccgcca
     3121 cccaaagtat tataaaataa atctgacaat tggaatgtct ctatgtgtaa aaataactgc
     3181 aaaaactcta taagacacga acataaaaaa acaaatttta taatctctga taaatttttc
     3241 caaaatcttt ttctgaagcc ttataccaca ttactagaat tataaatgga ataaacagaa
     3301 taacattttc aaaacattct gtagttaatc taatctcccc tgtcgtttca ttcacagagt
     3361 aaatccacca actaccaaac acattagaca aaggatttag ccaaacatat ctatccatca
     3421 gtgttctaaa aagaatcata tttgtaaaaa acacaagcaa aaacattttt ctaaatgagc
     3481 ttgattttct gaaataccga atccagacaa gaaaagattt tttcaaacct aatcctgatt
     3541 tctcctcagc catcgcaaac aagtataaaa acatagccaa aactgcaatt aaaatcgaaa
     3601 cccaaatatt tgatacaatg cagataacac attaaccaaa atagaagata tgattttctt
     3661 cataaactct cctttcatca tgcgctaaat gagtaccatc aagaaggttt cgtaaaagga
     3721 ggtacccact ctaacatgaa taacctcggc cagcgaaaat aaaactcaac agaattccta
     3781 tctctatgct gctataattt caacaaattt acatgtacca gttactaatc taatataatt
     3841 ttttcagcaa ggtaaatttc acactacacg catacacaaa aactgtagaa attaaaatta
     3901 ttatcattcc acaagtcatt tctattaatg aatttgtaat ccccaatttt gggaaaataa
     3961 aatttaaagc agctattata tacggatgta aacaaaataa taacgtacta attcttcgca
     4021 gatgtactgt tatttttata aatgtactat ctttaggctc agtgatactg atacaactac
     4081 cagtgctaat ggaatggaaa atattaacaa attattgact tctcgcaaag ctatttgatt
     4141 tatgatgaaa ctttcaccaa ctaataagca tcctatagct aaaacagcac ataagataaa
     4201 attacggtat ttaaagacct tattaagtat agtgtttttg tggaaggcta agtgttgccc
     4261 aataatgacg catggtacac ctataaataa tccattacga gttgttacaa acacgctttt
     4321 ataacaatat ctaatatact cgatggaatc attcccagat aatataaacc aataagattc
     4381 aaatgctaaa cctactacgc atagcaacaa accactgaga aactttattt tagaaacctg
     4441 gcttttgcgt actaaccata gagcaaaagc cagtgcaggc aagaaccaca ggtgatagta
     4501 actaccaata aacagaaaat ctctgatata caataataca tctgttttcc agctaaaatc
     4561 tgactgtacc caattcttta atactaatgg aatatataat atagaccaaa taatgtaaat
     4621 tttaagtact ctgtagattt gtttagataa tttagcggat gtaacttcac cactacacaa
     4681 caaaaaggaa ctgatgatga aaaaagtagg aacggcaacc gtactcagtc ctgcaacaat
     4741 accaatacta aatgcttaat ctggagctaa atagttttga cctaaatgac gaacaacaac
     4801 taaaatagcc attagtattt ttagaatatc tatcgaatag tactccccca cacacgcagc
     4861 tttataatta acattttcta tgattatatt ttccctattc atttaagaac acatcgagct
     4921 cttctggagt accgagtacg tgaacttttg atttatctac tatactaatg gttaccttca
     4981 tgcccttctc tatcatataa ttatatagcg gcgcaatata tttctcattc ttttccagat
     5041 gcctttcatc tgcataatat tcttcgtaga gtgatgcata taattttgca gaagaaaaat
     5101 aatatgcacc taaagtacaa ttgttagaaa ttctaacttt ttctcttact tctactacat
     5161 caccatattc gttcaacttg gcaaaactcc aatgatcacc gtccgcatgg aaacacggaa
     5221 tatgtccttc tccagaaata tcaacgtatt tcatctcacc tggctcaaca tatgtatcta
     5281 tgttataaac cataattgca ttatcaggtg cgcaataagg aattgcaagc atacatgtag
     5341 tggcttgtcc atcagtcata tagtcaattt caactacctc gacattttca attccataca
     5401 gactgcaatg ttctttgata aaaacagtag catcatcttc cttacgcaca acaaaaacat
     5461 acttttctac atgtgaatta taatcaagca agctgtctaa ggaccaatca aataaggttt
     5521 taccttttgc ttcaatcata aatttgggca cagtgtagcc aactttacga aagcgactgc
     5581 caagaccagc cattgtaata ataaccgtca tcttattcac tttataaagt ctcctttact
     5641 cagatttcaa tttggatttt ttgcaaatct catcgagctg atccagggta cacgataaaa
     5701 attcatccgg acgaactgtg cgatcatcaa cataaaaccc tttatggcct ggccaaggct
     5761 ttccataaag aatttcatca tacggaatct cccacttatc tagccaagcc aacaagattt
     5821 ttgcagtatt tgcattgatc acacctaaat taccatgata agaattcata ttgcgagatg
     5881 tgaacagtgt aatctttgct ccgttctcct tatagtatcg caacttctcc accatattag
     5941 catacggaac taaatcttca tatttctcat ctttcttttt gatggggcac aaagttccat
     6001 caatatcaaa aacaaatgcg taatctttaa acatataact aagacatctc ctttatttta
     6061 gccaggcaat ttatatgccc ggctttacac tgatcaaata gaaaaacaat agctcaaagc
     6121 attaacaata aatatccggg acaactcgtc ctaaaatttc caaaccagtt gctaacatag
     6181 ccatctgatg gtttaagctt tctccatgca aaggaatcat acttaaaaac agcaaagcct
     6241 caataagttc aatttttttc aaatcagaac ctatttcatc tttgaagaca gagtaaaata
     6301 cttcatataa atcataatct cgtttacgat cttgaacaat atagtctatg attgccttct
     6361 ttggatcata ttttattgta aacaagtcct taattataaa gtcatatttt ccatcaacac
     6421 tatgaaacaa ttttgccagt tcatatctat aatcaccata aatatcaaaa tctccaaact
     6481 tacctcgcgg atctatgact ttaatgaacg aaaatgtgtt atctaccata atattggcaa
     6541 agcataagtc accatgaata atattaaatt gcgtaatgtc aaacaattct cttggaacac
     6601 ttacgctcaa taaagcttct atctggtcca aagatttata tctgactcca tttatttgaa
     6661 tatcagaaga aaagaattct gtgaaccggg ggtcctttct gagcttattg aatctctgaa
     6721 atgttttatc gagatacata tctttcaatg acttttgaat attatcccct gaaacagagt
     6781 atctcttaaa gtctgagcat acaaatcgta ttctattaaa gatatctatc cattgttttt
     6841 ttgttaagtc actatag
//
LOCUS       DAJSMI010000140         6794 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJSMI010000140 DAJSMI010000000
VERSION     DAJSMI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.142"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(83..916)
                     /locus_tag="ON430_002259"
     CDS             complement(83..916)
                     /locus_tag="ON430_002259"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR3220161.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(1108..1812)
                     /locus_tag="ON430_002260"
     CDS             complement(1108..1812)
                     /locus_tag="ON430_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3220162.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(1835..2461)
                     /locus_tag="ON430_002261"
     CDS             complement(1835..2461)
                     /locus_tag="ON430_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3220163.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            2787..5162
                     /locus_tag="ON430_002262"
     CDS             2787..5162
                     /locus_tag="ON430_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR3220164.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(5186..5677)
                     /locus_tag="ON430_002263"
     CDS             complement(5186..5677)
                     /locus_tag="ON430_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCR3220165.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            5864..6784
                     /locus_tag="ON430_002264"
     CDS             5864..6784
                     /locus_tag="ON430_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3220166.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT         2191 a         1239 c         1314 g         2050 t
ORIGIN      
        1 tttttattat ttataatgat tattattaaa gaaaataaaa aaccagaaca tttctcaaat
       61 aagagaagtg ttctggtttt ttctacattg caataaacca ataaccaatc aaaatgatac
      121 caaggataat acgataccag ccaaaagcgg taaagtcgtt tttcttcaaa tagcttagta
      181 agaatttgat agctaagacg gaaaccacaa aagatacgaa tgttcctaca agaagaacaa
      241 cagtttcttc cattttgaat gaattccctt cagctaaata tttgacgatc ttcaagccgc
      301 ttgcgccaaa catgacagga atccctaaga aaaatgaaaa ctctgtagcg acaaaacgcg
      361 aagcaccaat caagatagat cctaggatcg ttgcgccaga acgggatgtt ccgggaacaa
      421 gtgccaagac ttggaataac ccaacaagaa gagctgcttg gtacgtaaag tcattcagag
      481 aagtccaacg aggtgttttg tttttgttgc gtttttcgac aataataaag gcaataccgt
      541 aaacgatcaa aacaatggca actggtaaga acttgtggaa atttgcttct aaccaatcat
      601 ccaaaggcaa accaatgatg atggctggta tgcaagcaac gatcacttta aaccaaagtg
      661 tccacgtatc ttttttttct accgttgatt tacttggaga aaatgggttt aacttgtgaa
      721 aataaagaac gacgacagcc aagatcgccc ctaattggat aacgacattg aacatcgaca
      781 taaattgtga actggcatcc agtttgatga attcatctgc taggatcaaa tgaccagtac
      841 tactgatggg cagccattcc gttattcctt caataatacc gagaatcact gctttgataa
      901 tatttgcaaa aaacataaat tccttccttc ccaattaaga tacatcttat agtataccgt
      961 tattttttga gaaacccaat ctgtttttta atattaagaa aaacttgaga aataaagcag
     1021 ataagtacac gaagagaaaa aagaagacaa agccctcgtt cagactttgc cttttatgat
     1081 tctctggtaa aaattaaaat atccccatta tagaaacgag tcgcgactat gtgttttccc
     1141 gttatcgata taagacgtat cattgatgcc gtggatagaa aatcgaccgt ttgcataagt
     1201 gacctttgtt acgctgccat ttaaaagccc agcacgaaca ggctgtttag gatcgatcag
     1261 attaagcaaa aaggcgatcg tcaaaccatg agatacgact aaaacatttc caccaccatt
     1321 tttttcgcag tgatgggcga tatcttcaaa tcctgtccat acacggtctc gcaatgtttc
     1381 ataagtttcc gcccacccag cggtatctaa gttgtataca gcattagcaa tttgttcaaa
     1441 agaaacttct gtggtaaaca tttcatcata tgttttaaaa ttcaatacac gagggatcac
     1501 accccacatt tccatgtcat atccaccttc aagagacccg aaacaccatt ctctaatcct
     1561 tttttcaata aaataaggaa tttcttttcc cttagaatgt tctcctagaa taatacgggc
     1621 agtttcgatt gcacgaccac tgtcactaga ataagcaaat gaaaagtcga cattttttaa
     1681 cccgcgacct aaataatgaa tgccttcttg tccttgcttt gtgaggggtg tatcacacca
     1741 gccttgtact cgttcaattg tattgaacat cgtttttccg tgtcgtacga tataaagtgt
     1801 agtttctgtc attttattct cctcctaagt cgttttaacg attgaaaaaa ggatttgtcc
     1861 ttttttcctg ttcaatcgtc gttgcatcgc catgtcctgg atagactgca aattcatcag
     1921 gtaatacaaa cagctgggtc cgaatactat gaagtaattg ttctaaattc cctgtataca
     1981 gatcggttct accgatactt cctctaaaca gggcgtcgcc agaaacgaca aaatcatcaa
     2041 aaataaagct gacgcttcct atagaatgcc ctggggtagg gacgacgcga aaagacatgt
     2101 ttcctaaagt ataagttttc atttcaaatt catattctgc aggcgagaca ataacatcag
     2161 caatatcatc atgtctaccg agtccagaaa gattcaaaat cggatcacct agccattttt
     2221 gctctagtgg gctgacataa actgggattt gataatacgc gcgtaaaggt tccactgcac
     2281 cgatatgatc gtagtgagta tgggtaagca aaatcgctac cggctgttgg tttgtttttt
     2341 tgatttcttg tttgattcgt tccgcatctt ctccagggtc aatgatcaaa agtgcttctt
     2401 cgttgtagac caaataacag ttttcttcaa tcattcctgt ttttaatctt tcgatatgca
     2461 tgtgcattcc tccttgatag ccaaaacttc tttcatccat tagtataacg cattcattgc
     2521 taggaaacaa aaacaggtac ttatttctct ttacttatct gaattatcag actatatgaa
     2581 aagtatttgg atattctaaa gagaaggaaa gtaagttttc atagcatttt aatatgtcct
     2641 tgttacatta gattcatcca aaaatccaaa ttctatatcc tatatccctg cctaatcagc
     2701 agggtgtttt tttatgtttg gaaagagagc tctttttgct aaactaggtt ttttcaggta
     2761 atcttagaga gaaagaggtg gtggaaatga tcaaacagtc aaaaatatct gtgcggcact
     2821 tagtagaatt tattttgcga cgtggaagta tcgacaatcg taaaaaaagt aaccatactg
     2881 cattagaagg agcgagaatc caccgcaagc tccaaaaaga tgcgggagaa aactatgaaa
     2941 aagaagtctt tttaaaaaca acagttcaat tagatgaata ttacctgatc gttgaaggcc
     3001 gtgcagacgg tattttcaaa agagacggta tttattatat agatgaaatc aaaacctccg
     3061 aaccacgatt tgaagattta gaaccagaac aagtggaact tttctttcat caagcaaggg
     3121 tgtatgccta tatctatagt caagaacatg atttacaaga aataaatctg caattgactt
     3181 acttccagac ggcagaagaa gtgatcacga gaaaggttga acatcagaca agagagcagt
     3241 tagaaacttt ttttaaaaag ctgactgaag attataaaga atggcttgtt tttcaagaaa
     3301 actggcgcac tgtacgcaat gcctctttga tggctttgaa attcccccat gatgagtata
     3361 ggaaaggtca gcgtgaacta gcggtagctg cttataaaac gatccgtacg aaacagaaat
     3421 tgtttgtaga agcacctacc ggaacaggaa aaacaatctc tacgttattc cccgctttga
     3481 aagctgtagg ggaagaagag ggagaaaaga tcttttatct aacggctaaa acaataacta
     3541 gacaagtagc tgaagatgct atgacagcat taaaagatac aggagctgaa gtaaaaagtg
     3601 tcactttgac ggcgaaagac aagatctgct ttttagatga gacgacctgt aatccagatc
     3661 agtgcccata tgccaatggt tattacaatc ggattaatga aggtttatgg gatcttttga
     3721 atcatgagaa tcagatcact cgtgaagtca tcgaaactta tgcaagaaag catacacttt
     3781 gtcctttcga actatcatta gatgtcagta tctggtgtga cgtaatcatt ggggattaca
     3841 attatttatt tgatccaact gtttatttaa gacgtttttt tgaagatgaa aaaaacgagg
     3901 actatctgtt tttgattgac gaagcacata atttggtgaa tcgttcccga gagatgtatt
     3961 cggcagaact atcttatgaa aaaacgaaac gaagtaagga agctatacca aaggaattca
     4021 aaaaacttca tcgccgtttc aacaaactat taaaagaatt tgattcgatc cgagaaatag
     4081 caaaagaaga tcattgggat tatcatcatc aaaaagcacc ggctgaatct ttagtaaagg
     4141 caggttatca attaagcgaa aaaattaaag aatggctggc agaatttcct gagcacccat
     4201 cacaagaaca attgcttcct tattattttg atttgcttca ttttttgaag gtcagtgaat
     4261 tttatgatga tcactacgaa acaacagtcg aaaaaacgta tcgcgatctg atagtaaaag
     4321 agttttgtat tgatccaagc ttgtttcttg agcagtcgtt ggataaagga aaaagcagta
     4381 ttcttttttc tgctagtttt tcaccactct cttattatca ggaaacactg gggggacaaa
     4441 aaagtctggc ttacaaactg ccgagtcctt tcccagaagg aaaccagcaa gttttcatcg
     4501 caaattacct agagacgact tatcgaaaaa gggaacacag tctggcaaaa ttagtagaga
     4561 cgattcatgc ttttgctgat ggaaaggtcg ggaattattt cgttttcttt ccttcttatc
     4621 aatatttaga tcttgcggtg gaagcattca gacagcaata tcccgacagc aatgtgctaa
     4681 tccaagaaac ggacatgaat gaggaagaac gcgaacgatt tttaacaaaa tttcaaacaa
     4741 atcccgctga aacattgatt ggattttgcg tacttggcgg gattttttca gaaggaatcg
     4801 atttgagagg ggaccgattg atcggcagta tgatcgtagg tgtaggtttg ccgcagatga
     4861 atcatgagca ggaactgatc aaatcatatt atgatgagaa ggaacacctt ggatttgctt
     4921 acgcttatca gcttcctgga atgaacaaag tcttgcaagc tggtggtcga gtgattcgtg
     4981 gcatggcgga tcaaggaatc gttgtattgg cagatcaacg attttctagt ttttcttatc
     5041 gacgtttatt cccacaacat tggcaaacag caagagaagt acgaagtgtt ggggaattgg
     5101 aagaaggaat acagcgtttt tggttatcaa aagaagcagt aaatcaaaaa aacgaggaat
     5161 gacagacgtc agcctcgttt tttgattaac gatagttagt gaattgcaac tcgattggta
     5221 agtcaagttt acgtaataaa gcaatcacag cttgtagatc atcacggttc ttacctgtca
     5281 ctctaatctg atcttcttgg atttgtgctt ttactttcag tttttcattt ttgatagctg
     5341 tagtgatttt cttcgcgttt tcacgatcga ttccgttgac caaatcggca gtttgtcgag
     5401 ctttgccacc tagagcgtgt tctgattcgg aaaaatggat atttttgatt ggcacatttc
     5461 ttttgatcag cttaccaaat aatacatctt tgatctgttc gattttgaaa tcatcatcgc
     5521 tcaatacaac tagattgctg ttctctaatt tgatttctac tgtagaatcc ttgaagtcaa
     5581 agcgattgga caattctttt gttgtttgct gaatcgcatt ttttacttct tctgtattga
     5641 cttctgatac aatgtcgaag ctagcatctt ttgccatagg aaccctcctg atatacatat
     5701 aaatatttac catacatcta tcttatcata ggattagggc agattcttac ctgtttgcta
     5761 aaattcttct catttgagtt cgaagtattt tattttaata gataaactgt tattcttaca
     5821 tttggaatac caaagtaggg gagaggtgct tgtgagtaaa attatgactg cgtatcataa
     5881 aaatgaaaga ttaaagaaaa tagtggttat tttgataacc ggattgaccg ctgcggtcgc
     5941 tttaaattat tttttgattc cagccaatgt cttttctgca gggatgaatg ggatcgctca
     6001 aatcatttcc tcgcttttat atgaatggtt tcatatcaag atagacacag gaacatttat
     6061 ttttcttttg aatatccctg tgtttgtatt agggtttctc aaagtaggaa aacgagcaac
     6121 gatccttagt tttgtgaatg ttgtatgtgt atcggtgatg acgatgctgc ttccagtcgg
     6181 acagatcact gataatattt tgatgaatgc cctagtaggc ggtgttttat taggtcttgg
     6241 cgtaggatta tctttaaaaa tgggctttac aactggtggg atggatatca tctctcttgt
     6301 actttcacaa acaactggaa aaacagtcgg aaactacatg ctgattctaa atggtatcat
     6361 tgtagtcatc gcaggcttct tctttaactg ggaaagtgcc ttgtatacga ttatttccat
     6421 ttatgcaatg actcaagtaa ttgatgccat ccacacgagt catcaaaaag taacagcaat
     6481 gatcgttacc gtcaagccag aagaggtaac agaagaacta gcaaaacgga tggtacgtgg
     6541 aatgacatta ctgccgtctg tcggtggcta ttcaaaaaaa caagggaaca tgatcatgat
     6601 ggtcatcacc cgctacgaaa tgtatgatct tgatcagatc gttcatgaaa tcgacgacga
     6661 tgcatttatc aatattttac caactcattc agtatttggc cggtttgcaa atgaaaacga
     6721 acaaaaaatg tatcgaacaa ctggcgtatt gccagaaata aaaaatcgtt cacaaaaaaa
     6781 atgaggaatc aggt
//
LOCUS       DAJSMI010000141         6686 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJSMI010000141 DAJSMI010000000
VERSION     DAJSMI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.143"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            155..928
                     /gene="sufC"
                     /locus_tag="ON430_002265"
     CDS             155..928
                     /gene="sufC"
                     /locus_tag="ON430_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR3220167.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            941..2227
                     /gene="sufD"
                     /locus_tag="ON430_002266"
     CDS             941..2227
                     /gene="sufD"
                     /locus_tag="ON430_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR3220168.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            2224..3459
                     /locus_tag="ON430_002267"
     CDS             2224..3459
                     /locus_tag="ON430_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3220169.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            3446..3916
                     /locus_tag="ON430_002268"
     CDS             3446..3916
                     /locus_tag="ON430_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCR3220170.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            3921..5312
                     /gene="sufB"
                     /locus_tag="ON430_002269"
     CDS             3921..5312
                     /gene="sufB"
                     /locus_tag="ON430_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR3220171.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5410..6552)
                     /locus_tag="ON430_002270"
     CDS             complement(5410..6552)
                     /locus_tag="ON430_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3220172.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(6603..>6686)
                     /locus_tag="ON430_002271"
     CDS             complement(6603..>6686)
                     /locus_tag="ON430_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR3220173.1"
                     /translation="KKYNFPYEKNSIIAKFLTDNHDKFKNN"
BASE COUNT         2131 a         1280 c         1416 g         1859 t
ORIGIN      
        1 atttctcatc ttttttatac ctattctaat ttagaaatca aaattgttta ataataaata
       61 aaagatattt gattttagta aggaaggtca ttgcgcttca ttctcaatta gtctaaaatg
      121 agtgagaacg aaagagaatt tgggggtata atgcatgtct gtcttagaaa taaaaaatct
      181 acatgtgtcg atcgaggaaa aagaaatcct taaaggcgta aacctaacaa tgaaaaccgg
      241 agaaatccat gccatcatgg gacctaacgg cacaggaaaa tctaccttat ctgccgctat
      301 catgggcaat ccaaactatg aagtaacaga aggcgaaatt ttgtttgacg ggaaaaatgt
      361 tttagacttg gaagtagatg aacgtgcgcg acttggctta tttttagcta tgcaatatcc
      421 aagtgagata ccaggtatca cgaatgcgga attcatgcgt gcagcgatca atgcgaaacg
      481 tgatgaaaac gacaaaattt ctgttatggc atttttgaaa aagttagatg aaaaaatggc
      541 tttactaaat atgccagaag aaatggctga acgttatctg aacgaaggtt tttctggcgg
      601 agaaaagaaa cggaatgaga tcctgcaatt gttgatgtta gaacctacat ttgcaatttt
      661 agacgaaatc gattcaggtc tggatatcga tgctttgaaa gtcgttgcta aaggagtaaa
      721 tgaaatgcgc ggagacaatt tcggtgcatt gatcatcacc cactatcaac gattattgaa
      781 ctatatcaca cctgatgtcg ttcacatcat gatggaaggc cgagtagtca tgacagggaa
      841 tgctgaccta gctaaacgtc tggaagcaga aggctacgct ggtatcagta aagaattagg
      901 aatcgactat aaagaagaag aagcgtaagg gggagcaaga atgaaagaaa aaaactggct
      961 cgattatctt gacgcggtaa atgatttttc tttaagtaaa ggcgaacctg attggatgag
     1021 aaccttccgt caagacgcac tagcgaaagc agatgaactg ccattgccgc atatcgatcg
     1081 ggtgaaattc catcgctggt cgttatttga tgtgaaagaa acacaaacca tcagtgaaac
     1141 aggcacgatt ccggcatttg atgcaatgaa agacaatcct gtgcttgtcc aacaaggttc
     1201 atggacgatt tttgaacaac tgcctgtgga attagcagaa aaaggagtca tttttaccga
     1261 cttgttcact gcaatgatcg aatatccaga actagtccaa gaatattata tgaaaaaagc
     1321 agtaaacatg aacgaagacc aattgactgc actccatgta gcttttatga acagcggtat
     1381 tttcctgtat gtgcctaaaa atgtcgtgat cgatgaacca ctcgagtcct tattcattca
     1441 agatggcgca tccgatgagc acttcttcaa gcatgtgttg atcgtggcag atgaacatag
     1501 tgaattttct tatctggaac gtttccagac aaccaaagaa caagtagcaa aatcatcagg
     1561 taatatcatc gtggaagtca tcgctaaagc tggttcaaaa atcaaatatt cagccgtaga
     1621 tcaattagga gaaaatatta cttcttatat gaatcgccgt gggcacattt tacgagatgc
     1681 ttctgtcgac tgggcaattg gtgtcatgaa tgacggtcat gtgatcgctg actttgactc
     1741 agatttagca ggagaaggtg cacacgcaga agtcaaaatc gtggcaatca gctcgggcag
     1801 acaaatccaa ggaatcgata cacgagtaac caataaagcc cctcatacga tcgggcatat
     1861 tttgcaacac ggagtgatcc gagaaaaagg aacattgacc tttaatggta tcggccacat
     1921 cttgaaaggg gcaaaaggtg cagatgccca acaagaaagt cgtgtcttga tgctttctga
     1981 taaagcacgt ggtgatgcga atcctattct tttgatcgat gaaaacgaag taactgctgg
     2041 acatgctgcc agtgtaggac gagttgatcc agaagaaatg tactatctga tgagtagagg
     2101 attgcataaa gaagaagcag agcgcttagt gattcgagga ttcctcggtt ctgtcttaac
     2161 agcaatccct gtagaacaag ttcggaaaga attagtcgaa gtaatcgaag ggaagttgaa
     2221 tggatgatcg atgctaaaaa gctacgcaat gattttccta tcttatttca agacgtcaat
     2281 gatgaacctt tagtttatct ggataatgca gcgacgaccc aaaagccaaa acaagtactc
     2341 caggcattag cgtactatta cgaacacgat aacgccaatg ttcatcgcgg tgtccatacg
     2401 ttagcagaac gcgcgaccca tgattatgaa caagcaagag aaaaagtacg ggcgttcatc
     2461 catgcaaaag aaacagcaga agtgctgttt actcgtggta cgacgacaag cttgaattgg
     2521 gtagccagaa ccttcggcga ccgctatatc aaagcaggag acgagatagt gctctcttac
     2581 atggaacatc attctaatgt tatcccgtgg cagcagttgg caaaacgaac aggtgctgtc
     2641 ttgaaatata tcgaactaac agaagaaggc ttcctggata tggaggatgc agcgaaaaag
     2701 attacggaca aaaccaaaat cgtctccatt gctcatgtat ccaatgtctt aggtgtcttc
     2761 aatcctgttc aagaattagc tgaacttgtc catgaaaacg gcggggtcat cgtagtagat
     2821 ggtgctcaat ctgccccgca catggccatt gatgtccaag cactagactg tgatttcttt
     2881 gctttcagcg gacacaaaat gtgcggacca acaggtattg gtgttttata tggtaaacga
     2941 aaatggttag aagaaatgga acctgttgaa tttggtggcg aaatgatcga ttttgtcgaa
     3001 ttatacgaca gtacatggaa agaactacct tggaaattcg aagcagggac accaaatatc
     3061 gccggcgcta tcgcattagg ccatgcaatc gattatttgc aaaaactcgg tatggacaat
     3121 atccatcgat atgaagaaga actagccgct tatgtgttgc caaaattaca agcaattgat
     3181 ggattgactg tttatggtcc acaagacccg aattttcgga caggagtcgt tgcattcaat
     3241 ttggatgggt tacatcccca tgatgtcgcg acagctttag acatggaagg ggtagccgtc
     3301 agagcaggtc atcattgtgc gcaaccgcta ttgaagtacc ttgaagtatc agcgactgct
     3361 cgcgcgagct tttatttcta caacacaacg caagacgccg accgtttagt tgaagcaatc
     3421 atggcaacaa aggagttttt ccaacatggg actatctaaa ttagacaatt tataccgtca
     3481 agtgatcttg gatcattctt cccatccgca tcatcgtgga agcttgactg actcgacaag
     3541 caagatcgaa ttgaacaacc ccacatgtgg agatgtgatc gaattgcaag tcgatgtaaa
     3601 agacggaatc atcaaaaata tcgcctttga cggcagtggc tgctccatca gtacggcaag
     3661 tgcctcgatg atgacagacg ccgtcatcgg aaaatcaata gaagaagcag aacatttaac
     3721 tgtagacttt ctacagcttg tccaaggaaa agaagtcaaa gatcccgacc aattaggcga
     3781 tgctgctatg cttggtggtg tagctaaatt tcccgctcgg atcaaatgtg cgacacttgc
     3841 ctggaaagca ctaggaaaag caatggaaaa cgatggtacc gcagctttcc atcattacca
     3901 caaggaagag gagtgagaac atgggcgtac cagaattaga agaatataaa ttcgggtttc
     3961 acgatgatgt cgaaccggtt ttcagtacag gagaaggctt gacagaagaa gtgatccgtg
     4021 agatgtcaag aatcaaaggc gaaccagaat ggatgcttga attccgtctg aaatctctag
     4081 aaacatttaa caaaatgcca atgcaaacat ggggccccga cctttcagac atcgatttcg
     4141 atgccatcaa atattaccaa aaaccaagtg acaaacccgc tcgtgactgg gaagacgtac
     4201 cagaaaaaat caaagataca tttgaaaaga ttggtatacc agaagctgaa cgagcttatc
     4261 tagccggtgc ttccgcacaa tatgaatcag aagtcgtgta ccataatatg aaagatgaat
     4321 tcgaaaagtt aggaatcgtc tttactgaca ctgactctgc tttgaaagaa taccctgaat
     4381 tattcaaaga gtacttttca aaacttgtac cgccaaccga caacaaatta gcagcgctga
     4441 attcagccgt atggtcaggt gggaccttta tttacgtgcc aaaaggcgtc aaagtcgatg
     4501 ttccattaca aacgtacttc cggatcaatg cggaaaacac ggggcaattc gaacgtacgc
     4561 tgatcattgt tgacgaagga gcaagcatcc actatgtcga aggttgtacg gcacctactt
     4621 attcaagtaa cagcctgcat gccgccatcg tagaaatctt tacaagaaaa gatgcgtatt
     4681 gccgttacac aacgatccaa aactggtcag acaatgtgta caacttagta accaaacgag
     4741 caaaagcata cgaaggcgca accgttgaat ggatcgacgg aaacctaggt gcaaaaacaa
     4801 cgatgaaata cccaagtgtt tacttagatg gaaaaggtgc acgaggcacc atgctctcca
     4861 tcgcctttgc aggagcaaac caaatccaag atacgggtgc aaaaatgatc cacaatgcac
     4921 caaatacatc tagctctatc gtctctaaat caatttcaaa agacggcgga gaagtaaact
     4981 atcgaggaca agtaaccttt gccaaaaaca gtgcgggttc gatctctcat atcgaatgtg
     5041 acacgatcat catggacgaa tggtcgaaat ccgatacgat cccattcaat gagatccaca
     5101 acagccaagt ctctttagaa cacgaagcca aagtctcaaa aatctcagaa gaacaactct
     5161 actatctgat gagccgaggc ctgaccgaat ctgaagcaac tgaaatgatc gtcatgggct
     5221 ttgttgaacc attcaccaaa gaacttccaa tggaatatgc agtcgaactc aatcgattga
     5281 tcagttatga gatggaaggg tctgtaggtt aataactatt tcattgctcg aaacgttgat
     5341 attaaagcgt ttcgagcgtt tttttatttg tattttaatg gttggctacg tttttggcta
     5401 cgtttggaat tattcgccca taaattttgc aaatcggtca ccagttttct taacagattg
     5461 tggtgtgaca tgagcataga tattcatggt agttttaata tctttatgac ctaatcgttc
     5521 ctgtacttct tttatcgacg caccactttc aaacaataag ctacagtggg tatgcctaaa
     5581 tccatgtgga gtaatttgag gctccatcgg tgtctttttg taaatccagt ctaaccaatc
     5641 gtttactact tgggggtagt acaacttgtt tgtgtttgta gtgaaaagaa actgttttgg
     5701 tgaattcgtg ttatagccat ataagatcat tattttcatt tgttcttgtt gccaaatacg
     5761 cagttgttta agtgtaaaat catcaagctg aatcgttctt gatgaatttt tagttttagg
     5821 tacttgtaga actacttgat taaactcgtc ttgagcgata gttttaccaa ttgttattgt
     5881 tttattgaaa tagtctatat cttcccattg aagcgccaat gcttcgcttt tacgcattcc
     5941 tgtataagct aataaacgga aaaaagtgta cagtttaact gaattatttt ctttagtatt
     6001 ctgtaaaaac agttgtaatt gatctttcgt gtaaaacttc aagggttgtt cttcttcaat
     6061 ttttcttgga agcaatgttt tagacatagg attatcctta cagacttcca ttgctactcc
     6121 aaatttaaat atttgagcag ttgtttttct caagaaacca tattgtttta atgttttgtg
     6181 ccattcattc acctgttttt ggcaatagct aacacttatt ttatccaatc gtaattcccc
     6241 aaaagcaggt aatatatggt ttttgcaaaa acgttgagca acggcaacag aagacggttt
     6301 tactcttgtt ctgtagttat caatccataa ttcatacaac tcttgaaaag ttgtaacatt
     6361 gattttaaac tcttgttttt ctttaaagtc tacaagtagt ctattaagtg ctgtttgcgc
     6421 ctcagctttt gatttgaatc ttctacgctt tgtacgtata tcttttccag tcttgggatc
     6481 tttccctaaa taagtttcaa atccccaata tttttcgcca ttttgtaatt tataactaaa
     6541 aaatgttgcc atttgattct ctccttactt cattattgca ggtagagaag ttatttgaag
     6601 aatcaattat ttttaaattt atcgtgatta tcagttaaaa attttgcgat tatactattt
     6661 ttctcatatg ggaaattgta tttttt
//
LOCUS       DAJSMI010000142         6632 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJSMI010000142 DAJSMI010000000
VERSION     DAJSMI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.144"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..611)
                     /locus_tag="ON430_002272"
     CDS             complement(<1..611)
                     /locus_tag="ON430_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3220174.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDP"
     gene            complement(628..2100)
                     /locus_tag="ON430_002273"
     CDS             complement(628..2100)
                     /locus_tag="ON430_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR3220175.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWINDPNGFIY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVMEGKVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMTAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQEKIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPHHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            complement(2114..2962)
                     /locus_tag="ON430_002274"
     CDS             complement(2114..2962)
                     /locus_tag="ON430_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3220176.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LTSFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            complement(2959..3825)
                     /locus_tag="ON430_002275"
     CDS             complement(2959..3825)
                     /locus_tag="ON430_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3220177.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            complement(3868..5181)
                     /locus_tag="ON430_002276"
     CDS             complement(3868..5181)
                     /locus_tag="ON430_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR3220178.1"
                     /translation="MKRRMLAIAAILALTAGGLAGCGSKEKSSGSSDSGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMESGDEMLTYAY
                     TPFVWSNGGDVVNADGTKAEDYFNDKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMLLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDGSA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQVIDTSLK"
     gene            complement(5323..6294)
                     /locus_tag="ON430_002277"
     CDS             complement(5323..6294)
                     /locus_tag="ON430_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3220179.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGFSPYPNETNNRKAGFYNICEKNKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            complement(6420..6632)
                     /locus_tag="ON430_002278"
                     /pseudo
     CDS             complement(6420..6632)
                     /locus_tag="ON430_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1976 a         1462 c         1147 g         2047 t
ORIGIN      
        1 taaggatcat cttctgcaac ctcttgtcct tttttcccta agcgtttttc gatggcgggt
       61 ccagcggcca tcccttctaa acagttgcca tgaaatggac agctgccagc aaagtcatct
      121 tttggatgag ggacgacgag catatgcccc atttctggat gactaaaccc ttcgataaag
      181 cgcccttctt gtaaggcacc gccgccaaca cccgtaccga tcgtatagta aacgacactg
      241 ctcaatcctt tgccactgcc gaagacatac tcaccatagg cagccgcatt gacatctgtt
      301 gtccaagaaa tcgggatcgg aaattctttt ttcatggtac cgatgaaatc aaaattttgc
      361 catgccaatt tcggggtcga ggtaatgtac ccataagtgg cggaatcgcg atggatatcg
      421 ataggcccaa atgagccaat cccaatccct gctaaatctg cttggtattg tttgaagaag
      481 tcaatgacta aaggcattgt ttcctcaggt gtcgttgtcg gaaagctcac tctttccttg
      541 atcgtcattt cttcatcgcc aatggcacac acaaattttg tgccgcctgc ttcaattgaa
      601 ccataaatca tcactgagct ctcccttcta tagattaatt aagccgcttt ccaatgaaaa
      661 ggttacttca ctgtctgcca gcaaaagaaa atcctgtcct ttttctgtct cataaaaagt
      721 tgtactcata gtcagacgat catttagaaa aagctcaata gctgaagtat cacggaaaat
      781 ttcgatggtt agacaattat tttccaaagg aatcgtcaca ctgcgctttt tcaacacttc
      841 cttttcagct cctgataaat catacggcat gtgttccctg gaagcagtaa agcactcccg
      901 ttcaacatca tagttaagca gtaatgtatg gtggggtccc ttcgcatagt gaagttctag
      961 tttcgttgcc tgttttaaat tgatttccaa gcgtaaatag tgctggtcat atactaagtc
     1021 cttcagtaag aatcgattct cttcagtctt cgcccattca atcttttcct gcggatcaat
     1081 tacttgataa attgatgaaa ctggccgttg caacaattga ttatcagcca ccctcagctc
     1141 tctaggcaag gtcatggaac ctgaccaacc atggcccaga tcattcgtag gtattttacg
     1201 ctgccacatt tgcatccaag cagtcattat tctttggcct gcttcgtttt cacaggtctg
     1261 gggggcataa aaatctaaac cgccatcgat ttcatgaaaa ttctctacta gaaatttgcc
     1321 tgtttgccaa tcgacagtac caatgaaagc aacagttgag ttcgtatttt ggtaagcatt
     1381 gccttgtgga gtcatttgaa taggagaaag aatcaagact tctttgccat ctagttcaaa
     1441 caagtctggg cattcccata caatgccttg ttctggagtt ccctccagca atacggaata
     1501 aaactcccaa gttagtaaat ctttggaatg gaacatcaca atttgtccac gagcatctgc
     1561 agttttagta gcgactacag taaaatagtc ttcttttcgc tgaaacactt tgggatcgcg
     1621 gaaatcttcg gtcgttgcaa cagaagctaa gtgatcttct gcgatgattg gatttccggc
     1681 atatttttca aagtgaatgc catcttctga aactgccaaa cactgaactt gtcgaacttt
     1741 cccttccata acattgtgtc ctgtataaat caaatacagc ttgccatcct tttctatagc
     1801 actgccggaa aaacagcctt ccttgtcata gctttcacct ggtgccagtg ctactggcaa
     1861 atgctcccag tgaattagat ccttgctttt agcatggccc caatgcattg gtccccagat
     1921 gctgtcgtaa ggataatatt gataaaacaa gtgatactcc tcttgataat aaataaaacc
     1981 atttggatca ttgatccagc caatcggagc catcaaatga aatttattcc ggtattcttg
     2041 attgactgtt tgagcatttt tctcaataaa ctcattggcc tttaaaagtt tttgcaacat
     2101 tcactattca ctcctatttt atacccgttc cagaaccgcc aagtccctgc agaatatatt
     2161 tttgtgcaac gatataaaca ataatcagtg gaatcgttgc aatggttaaa acagccatca
     2221 cttgattgat ataaactggc tgtgtagtat tgatcgtagt aattgccact tgcattgaaa
     2281 atttgctgct gtcagttaag accattaacg gccaaatata atcattccag ctggagataa
     2341 aggataagac acctactgtg gctatggctg gtttagacat tggcagcatg atgtgaaaga
     2401 aaattttaac aatatttgca ccatctaatt tcgctgattc aatgatttct tctggaatcg
     2461 cgataaagaa atttctaaac aaatagatat taaacgcgct ggccatcgct ggtaaaatca
     2521 ctgccaagcg agtattcact aagcctagtg aattaacgat ggtaaattgc ggaattaaaa
     2581 ccgtctccat cgggacaatc agcagagcta acaaagctcc aaagataatt ttcttgcctg
     2641 aaaaattaac cttcgcaaag gcaaaacccg ccaaagagtt tacaatgatt gacccacagg
     2701 caaaggttaa accataaaaa atactgttta ataaataagt tcccacactg aaccgggaaa
     2761 taacttcttg ataagtttta aaccaattag ctggattcaa actaggtaaa aaagctttga
     2821 aggaagttaa attttgatag acatctgctt caggcttcat agaggaaact accatccaca
     2881 gcattgggaa aatgaataat gccgctaaaa ggaacagcaa acaatattct acgatcatta
     2941 gcggtccaaa tttcttagtc atgctggatc atcctccttt accaattttc tttgagcaat
     3001 tgtaattaaa ccaatcatgg tggtaaagat taacgcaatg gagctggagt agcctaccat
     3061 acggtctgta aatccagttt ggtaaatgta ataaaccatt gtcatagtag aattcattgg
     3121 tccaccttga gtcattacca ttggctgaat gattagttta aaggccgaaa tcaatgtggt
     3181 aagtaaaata aaaatcgccg tgggctttaa taatggcatg gtgatatagc gaaattgctg
     3241 aaacttagaa aaaccatcaa tttcggctgc ctcataaacg tcttgcggaa tgttttgcat
     3301 tccccctaaa aacagcagca tctgatatcc tgcaccttgc catgccgata cgaaaacaag
     3361 cgtatacatt gcttgtttgg gactagtcaa aaaaggctgc gaagcaattc caatctttgc
     3421 aaatagcgta ttaaccagac cttcattagg atttaacaag tacaaccata aaatcgaaat
     3481 aacgactaag gacatcacga ctggcgcaaa aaaggctact ttaaaaaaga tatttccttt
     3541 acgatttttg ttgataataa gtgccattcc taaagctgcc cctaattgga ccggaataat
     3601 ccaaacaaca aatttagccg tgttccaaat actttttaca aaaattggat cagcgaacag
     3661 tcgcttaaaa ttttctaagc cgataaattg tctagcatct ggcgttaaca aataataatc
     3721 agtaaaggca taataaatta ccatgattac gggtatgatc agaaaaatcg tcagcagcag
     3781 caaagctggc gctagaaatc cgtaacctat caaattgtct ttcattgtcg gtttttttaa
     3841 cacttgattg gcccccttta gaaacttcta ttttagtgaa gtatcaatta cctgttgcat
     3901 ttcagcagcg cgactatctg ctactttttg tacatctggg ttttcctcga agtagctaat
     3961 atcttgaact gcttgttgga atgctctaga tacttgcgga taagctacaa ccactgggcg
     4021 ggcatgtcca gaaatttggt tttgcttaat taatacctgc attccttccg acacttcatc
     4081 tttaattaaa tcaactgttg aatttctgat aggtagaaca ctgttcccta aactcataat
     4141 acgactagat tctgtactgg ttgcaaactt aataaaggcc gcagaaccat cctttttctc
     4201 tgttttctca gtcattgcta actgccagct gccggttggt gaaactaact ccccagttgc
     4261 atctgaaacg gggtaaggca aaataccata atcaatctct tgatagtttt cctctaaatc
     4321 tgccattgtc caagaaccgc tcagcagcat ggcatatttg ccagtttcaa atcctttttc
     4381 aataggactg attgtggtgt aacctttttc aaccaattct tgaataaatt ccaacgcttc
     4441 aacggatttt ttatcgttaa aatagtcttc ggctttggtt ccatcagcat taacgacatc
     4501 gccgccattc gaccatacaa atggtgtata tgcgtaggtc agcatttcgt ctcccgactc
     4561 catttgcata tcaattgcag gcgttttgta tttatcagtc agcttttggc aaattgcttg
     4621 aaattccgtc caagtccaag gatcttctaa cgtcggcaat tgatcttctg taattccagc
     4681 atctttgaac aacgacttat tgtaatagac acccacactg gactctgaga aaccaaacgc
     4741 atacattttg ccatcgtagg ttccttgctg aataatgctg tctaatacat catccatgtc
     4801 cgcatctttc aaataatcat ctaatggagc aatgactcct gaatgagcat aagctgcagt
     4861 attcggtcca tctaacgtaa taacatcagg aagggtattg gttgtaattg cagcatttac
     4921 tttatcctca tatccaccgc cgcttccgct acgaggaata tactcagtaa cagctttata
     4981 ttcaccctcg tattcatcat taaaattgtt cacaatctct gcccaagctt ttccttcagg
     5041 cgtttcatca gaaaactgaa cccaaatctt aatttctcct gaatcactgc tgcctgaact
     5101 tttctcctta ctgccacatc ctgcaagtcc tccagctgtt aatgctaaaa ttgctgcaat
     5161 tgctaacatt ctccttttca tcgatgttcc tcctattttt tgaaacgttt ccgttttatt
     5221 ctccaattaa ttggtaacgt ttccaattaa ttggttttaa gatatcattg aaaccacttt
     5281 ctgtcaacct atctcaaaaa aaaatcgtta taaaccggtt ttttactctt tagtcgtttc
     5341 tccagcgtga aagaaaaccg gtactaccac acgcttaggc ggttcttcat ttaaaatcat
     5401 cgccatgagt gtttctactg aaaccttggc catttcctct atcggctgta caatcgtcga
     5461 tagtaaatag tctttttgca acgatagctt gactccatca aaaccaataa tttgaaaatc
     5521 cttaggtgct tccttcccta gttttttttg ggcttttatc acatccaaag ccatgaaatc
     5581 attgatggta aaaatgctat caatttcagg attatccgaa aaaaaagcga gcaattgttc
     5641 ctccaaattg ctaattggtt caggcatatc caataccttc acagctattt tatttttctc
     5701 acaaatattg taaaaaccag ctttacgatt gtttgtttcg ttagggtaag gtgaaaaacc
     5761 cccaacatag cacaagtttt tacagttacg agtaagtagt tcttgagcgg caatttgccc
     5821 acctaaataa ttatctgccg ttacataaga aacctgttcc gaaaaatggc ggtctatact
     5881 gacaaatggt aaatttgatg atacatattt atcaatgtct gaataggtaa tacctataat
     5941 tccgtctact ttactctgct tgaccatttg gatatattct ttttcttttt ccgcactgcc
     6001 gttcgcatta cacaaaaaaa gcttataatt tttttggctg agagcttcct ctacatagta
     6061 ggcaaactcg gaaaagaagg gatgccaaat cgtaggtaaa ataagagcta ttgtattgct
     6121 gctattcgtt ttcatacctc tggcatattc atcaggctca taattaagct cactaattgc
     6181 agccattact ttttctttgg taacctcttt cacctttact ccatttatta ctctagatac
     6241 cgtacccaca ccaactcctg ctaacgcagc aacatccttc atagtagttt tcacttcacg
     6301 aacaactcct ctcaaaagaa tttcaggtat tattatacca aaagaagaaa acgattccaa
     6361 attagcagga atatttttat tttaaaatag atattagacg tgaagctttt cgtaaaaaaa
     6421 ttttcgtatt cagatttacc ggattgactc tgtcgccaat ataggccggc atactttgtc
     6481 aattttattt catcagaaaa acgttcaatt ggaccaattt cagcgagtat tccagcagcc
     6541 taaacaggac caatacctgg gatactggtt aaacattgat actccggtag caccacgact
     6601 aagtcgtcaa tggctttatc taagtcttta at
//
LOCUS       DAJSMI010000143         6547 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJSMI010000143 DAJSMI010000000
VERSION     DAJSMI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.145"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(1273..1938)
                     /locus_tag="ON430_002279"
     CDS             complement(1273..1938)
                     /locus_tag="ON430_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3220180.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2118..4100
                     /locus_tag="ON430_002280"
     CDS             2118..4100
                     /locus_tag="ON430_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCR3220181.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ON430_002281"
     CDS             4104..4742
                     /locus_tag="ON430_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3220182.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ON430_002282"
     CDS             4770..5198
                     /locus_tag="ON430_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220183.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..>6547
                     /locus_tag="ON430_002283"
     CDS             5655..>6547
                     /locus_tag="ON430_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR3220184.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDS"
BASE COUNT         2242 a          995 c         1212 g         2098 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctag
//
LOCUS       DAJSMI010000144         6527 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJSMI010000144 DAJSMI010000000
VERSION     DAJSMI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.146"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..391
                     /locus_tag="ON430_002284"
     CDS             <1..391
                     /locus_tag="ON430_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR3220185.1"
                     /translation="NQENFINNIKKFIVHLLENVPTDIEKLNFSDYQEQKEKEAEKNI
                     VGKCPKCGNNIILKKSFYGCSNYPECKFTLAEHFRKKKLTKTNVKELLEGKETLVKGI
                     KNKEKKPYNAVVKIGEKGYIDFISFSK"
     gene            554..1219
                     /locus_tag="ON430_002285"
                     /pseudo
     CDS             554..1219
                     /locus_tag="ON430_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            1255..1500
                     /locus_tag="ON430_002286"
     CDS             1255..1500
                     /locus_tag="ON430_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3220186.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1603..2472
                     /locus_tag="ON430_002287"
     CDS             1603..2472
                     /locus_tag="ON430_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCR3220187.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2453..3187
                     /locus_tag="ON430_002288"
     CDS             2453..3187
                     /locus_tag="ON430_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3220188.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3220..4128
                     /locus_tag="ON430_002289"
     CDS             3220..4128
                     /locus_tag="ON430_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HCR3220189.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4125..4667
                     /gene="sat4"
                     /locus_tag="ON430_002290"
     CDS             4125..4667
                     /gene="sat4"
                     /locus_tag="ON430_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HCR3220190.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4760..5554
                     /locus_tag="ON430_002291"
                     /note="aph(3')-IIIa"
     CDS             4760..5554
                     /locus_tag="ON430_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCR3220191.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            5830..6402
                     /locus_tag="ON430_002292"
     CDS             5830..6402
                     /locus_tag="ON430_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR3220192.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         2281 a          963 c         1412 g         1871 t
ORIGIN      
        1 taatcaagaa aactttatta ataatatcaa gaaatttatc gttcatttat tggaaaatgt
       61 acctaccgat atagaaaaat taaatttttc tgattatcag gaacagaaag aaaaagaagc
      121 agaaaaaaat attgttggca aatgcccgaa atgcggaaac aatattatac tgaaaaaatc
      181 gttttatggt tgttcaaatt atcctgaatg taagtttact ttagctgaac attttagaaa
      241 gaaaaaacta accaaaacga atgtaaaaga attactggag ggaaaagaaa ccctggtaaa
      301 aggaatcaaa aacaaagaga aaaagcccta caatgccgtt gtaaaaattg gggaaaaggg
      361 atatattgat tttatctctt tttcaaaata aacatggggc tgacccaaaa gtagctccta
      421 tatgaaaaat agaggtcttt ccatttagat agtagtggaa agacctctat ttatcttttg
      481 aaagtgaata caaaaataaa gcacactctg ctataatata ggtgattaat ccatttgaga
      541 gaggtgcttt atcatgtatc aaaattatac cataaaccaa ctttgtttgc ctatagatct
      601 tgaaattaaa ttagaagaaa atgattttgc tcatgccatt gtgcagtttg ttgactctat
      661 tcccgatgaa gtctttcttc cttattatca atctatgggt agaccccaat atcatcctcg
      721 tatgctactt tccattattc tttgtgccta tattcaaggg gtttattcgg gccgccaaat
      781 acaaaatatg ctgatagata gtattcggat gcgctactta tctcaagagc aattccctaa
      841 ctttcgtact attaatcgtt ttcgcgttca tccgattatg aacgatattc tagatcattc
      901 atttgttcaa tttcgtgagc tactggttca aagtggctta atcagtggca gtgctctata
      961 cattgatggt acaaaaattg aagcggatgc gaataaatac tctttcgttt ggaaaaaaag
     1021 tattttaaag tataaggata gccttgatca aaaagccctt gaaaactacc aacaaatggt
     1081 ggaaacaaaa atcttacctg agttaataga tgatttaaag gatgaacttt ctatagaaga
     1141 gattgaaagg attcgtcaat ctttggaaga aaaggaaaat caattaattg aagcgattga
     1201 ccaaaccgaa acagtagaag ggggctgacc cattattaat aaggagtgga caacatgatt
     1261 gatgtaaatg agctattgga agaagctatt agagaaactg aaaatctaaa tgatggcgaa
     1321 gtttttcttg ttaaagattt attcaaggga tatgtgggga ataggatacc cagaaaggat
     1381 cgacttctgc ttggaacgtt atttttaaac catgttagca aaatgaatgg taatttaaag
     1441 gctattgaaa agacttcatc taatcagcag aggtataaaa agacaattga taagttttaa
     1501 ctatcttttg tgaaattaaa aaaattataa atatgttgag tagaagcaaa agatttggct
     1561 tggaattttt acagcagtga atcgataaaa ggagcatgct tgatggtaga taatattatt
     1621 aaatcagtag cagagaaatt atcctctctg tcttatatag aaggtattgt tttaggtggt
     1681 tcacgtgcaa ggggcaccca tacagaggat tcggatatag atatcggcat ctattacaat
     1741 tcagaatcat ttgacataaa tactattaat caattcgcta caaagctgga tgatgagcat
     1801 agaaataacc ttgttgtacc tcccggagca tggggtgatt ggattaatgg cggcggatgg
     1861 ttagtcataa acgggtatca tgtggattta attttacgtg atattaaacg tgtggaacaa
     1921 ataatgaaag atacagagca cggaattgtt actgccaatt atcagactgg gcatccccat
     1981 ggttatatta gtgcaatgta tcgaggagaa ttagcgatta gcaaaatact atatgctaag
     2041 aatgaaagct tatgcgaatt aaaaaaacag gcagaaactt atcccaatgc tttgcagaaa
     2101 agtttagtta acttttttat gtttgaagca gggttctctt taatgtttgt aaaagcaaat
     2161 tcgggaacag acgataaata ttatattgcg ggtcatgttt ttcgtatagt ttcatgttta
     2221 aatcaagtgt tatttgcatg taataatgct tattgtatca acgaaaagaa agctataaaa
     2281 ctgcttgaaa cttttgaaca taaacctgaa aaatataccg agaaggtaaa tcatattttt
     2341 gaagtactcg gtatctcact ttttgaatgc tacgacatga ccgagaagct ttataatgaa
     2401 gtgaatgaaa ttgtatcgga gataaataac tttttaaacg aggagagttc agatgaaaga
     2461 aaacaaatat gatgataata tattttttca aaaatacagt caaatgagtc gctcgcagaa
     2521 aggactggct ggtgcgggag aatgggagac tttgaaaaag atgctacctg attttaaggg
     2581 taagcgtgtg cttgatttag gatgcggcta tggatggcac tgtatatatg cgatggaaaa
     2641 cggtgcttcc tctgtagtag gtgttgatat ttctcataaa atgctcgaag tagcaaaagg
     2701 aaaaacccat tttccacaga ttgaatatga atgctgtgcc atagaagatg tggatttccc
     2761 agaggagagc tttgatgtaa tactaagttc gcttgcgttt cattatgtag cagactatga
     2821 gaatttaata aaaaagatat ataggatgct gaaggctggt ggcaatttag tttttacagt
     2881 tgaacatcct gtttttactg ctcatggaac acaagactgg tattataacg aaaaaggaga
     2941 aatactgcat ttcccggtgg acaattatta ttatgagggc aaacggacag ctatgttttt
     3001 ggaagaaaag gttacaaaat atcatagaac actgaccaca tatctaaata cactgctttc
     3061 aaatagtttt ataataaatc agattgtgga gccacagccg ccagagaaca tgatggatat
     3121 tccggggatg gcggatgaaa tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa
     3181 gatgtaataa tatagaaaaa ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg
     3241 atggatttag tactttcttt agcagaacag gatgaacgta ttcgaattgt gacccttgag
     3301 gggtcacgcg caaatattaa tatacctaaa gatgaatttc aggattatga tattacatat
     3361 tttgtaagtg atatagaacc gtttatatct aatgatgact ggcttaatca atttgggaat
     3421 ataataatga tgcaaaagcc ggaggatatg gaattattcc cacctgaaga aaagggattt
     3481 tcctatctta tgctatttga tgattacaat aaaattgatc ttaccttatt gcccttggaa
     3541 gagttagata attacctaaa gggcgataaa ttaataaagg ttctaattga taaagattgt
     3601 agaattaaaa gggacatagt tccgactgat atagattatc atgtaagaaa gccaagcgca
     3661 agggagtatg atgattgctg caatgaattt tggaatgtaa caccttatgt tattaaagga
     3721 ttgtgccgta aggaaatttt atttgctatt gatcatttta atcagattgt tcgccatgag
     3781 ctgctgagaa tgatatcatg gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc
     3841 aagaactata agtttattga aaggtatata tccgaggatt tgtgggagaa acttttgtcc
     3901 acctaccgga tggattccta tgaaaacata tgggaagcat tatttctatg ccatcaattg
     3961 ttcagggcgg tatccggtga ggtggcggaa aggcttcatt atgcctatcc ggagtatgat
     4021 aggaatataa caaaatatac cagggacatg tataaaaaat acactggtaa aaccggctgc
     4081 ctggatagca catatgccgc tgatatagaa gagaggcggg aacagtgatt acagaaatga
     4141 aagcagggca cctgaaagat atcgataaac ccagcgaacc atttgaggtg ataggtaaga
     4201 ttataccgag gtatgaaaac gagaattgga cctttacaga attactctat gaagcgccat
     4261 atttaaaaag ctaccaagac gaagaggatg aagaggatga ggaggcagat tgccttgaat
     4321 atattgacaa tactgataag ataatatatc tttactacca agacgataaa tgcgtcggaa
     4381 aagttaaact gcgaaaaaat tggaaccggt acgcttatat agaagatatc gccgtatgta
     4441 aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata gaatgggcaa
     4501 agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt atagcttgta
     4561 aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact
     4621 ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca
     4681 gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa
     4741 aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat
     4801 cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct
     4861 ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac
     4921 ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc
     4981 aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga
     5041 tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct
     5101 gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa
     5161 tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt
     5221 ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt
     5281 tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag
     5341 caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag
     5401 ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga
     5461 agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat
     5521 aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg tctaaaaagc
     5581 tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca actgtccata
     5641 ctctgatgtt ttatatcttt tctaaaagtt cgctagatag agttctatat tagagatgta
     5701 aagagttttg gtgaagaggt ttgtgagtat ggcttttata accagggtgc aatgagaagc
     5761 actaacagca gctagcttaa gaacgcatag gatatacttt ttatggaagt cacagaggag
     5821 gaataaaatt tgaacaggat taacagagta acctctattc ttattcagct gcaatcaaaa
     5881 aaaataattc ctgccaaaga aattgcacag cgatttaata taagcttaag gacagtttac
     5941 agagatatcc ggacacttga agaagccgga ataccaattg gatctgaggc cggaaaagga
     6001 tattttcttg tagagggctt cctacttccg ccggtaatgt ttacagcggc ggaagtgggt
     6061 gcattgatta cagcagggaa atttttaaat tgccatggag atgaatcatt tataaaggat
     6121 tttgattcag ctatgtataa aatcaaatct attttgaagc atggtgaaaa aaactatgca
     6181 caggagttgg agaacagtat taacgtgtac agcacatctg gacagaaaaa tacattggcg
     6241 gataacgtta ttgcagcaat tcagactgca atctgcaata aaagggtaat atcaattcaa
     6301 tatcctgcat caggagggca agaaccggaa agcagaatga tagagcctat atcactgggg
     6361 ttttatgaac agaactggta cttaatcggt tttgcaggct aagaaatgaa tataggaatt
     6421 ttcgtgtgga tcggattaaa agcatttgca tagaagaaga gctctgtcaa agccatgatg
     6481 gctctttgga acaaatatta aaacaaatgc tttcatataa aaaacta
//
LOCUS       DAJSMI010000145         6455 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJSMI010000145 DAJSMI010000000
VERSION     DAJSMI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.147"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(146..745)
                     /locus_tag="ON430_002293"
     CDS             complement(146..745)
                     /locus_tag="ON430_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCR3220193.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            1149..2270
                     /gene="mnmA"
                     /locus_tag="ON430_002294"
     CDS             1149..2270
                     /gene="mnmA"
                     /locus_tag="ON430_002294"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR3220194.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            2516..3937
                     /gene="arcD"
                     /locus_tag="ON430_002295"
     CDS             2516..3937
                     /gene="arcD"
                     /locus_tag="ON430_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCR3220195.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            3951..5069
                     /locus_tag="ON430_002296"
     CDS             3951..5069
                     /locus_tag="ON430_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR3220196.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPEVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5129..6421)
                     /locus_tag="ON430_002297"
     CDS             complement(5129..6421)
                     /locus_tag="ON430_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3220197.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
BASE COUNT         1985 a         1161 c         1403 g         1906 t
ORIGIN      
        1 acgaatagta tggaatggag ttgtgacaga agtcttgtta caactccatt tctgatccta
       61 atgttcctgt ggcaataagc ctactcaaat gaaaaatatg agtaattatc tttatttgtt
      121 taatacttga tatagggcag ttttttcacg acctatcgta cttcctcttt gcttcataga
      181 tttctttgat ttgttgctca gataacggag tgccaaatgg tagtttttcc tttaagagtt
      241 ctctttccgg tgcgtctacg cctacattga catcgttctc gataggtacg ctgtaatgat
      301 ggatatgtcc atgaagattg cggatagtgt cgttaggccc gagcaacatc ggataatgag
      361 tcaagtaata ttgttgatgg tcgaatttaa cgatggcacc tacatcataa aaataaaatt
      421 tgggacttcc gtcagtaagc ctaggatcat ttttttctaa ataacggaaa aaagcgcgac
      481 tgtcatggtt ccctttgatc aagtggattg ttccttgcag ttgagtgacc agttccaaaa
      541 tttctgcatt tgctttttca tattgcggat gcatagcaag gtcgcctaga tgataaacat
      601 ggtcggtttc ttttattact gcattccagc tagtgatcaa ctgttggttc atttcatcca
      661 catttgcaaa gagccgcgga gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt
      721 gcatatcact gataaagtaa ttcatgtatc ttttgcctcc cttttttctt agagtaaaca
      781 aaatcactta tctgagcaac tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc
      841 agctttaagc gataagaagc aattttgaaa aatagttttt cacaatttat aaaattattc
      901 cttattgccg aaaggctggc cctttttatg agtatgccaa ttttttatat cgaatgctca
      961 aaagatgagt gattttttgg ttgactatca tgtttgtgta ctgaataggt ccagtagaac
     1021 agtattttta cgacttgata attaagggat gaaattttca gaaatatgac gtataatgta
     1081 aaatactgaa aagttgcgac cttcaactcg tagatgtttc agaatctaat tgaaatgatg
     1141 gtgatatcat gacagacaac agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg
     1201 actcatctgt aacagcactt ttgttaaagg aacaaggata cgacgtgatc ggaatattca
     1261 tgaaaaattg ggatgacacg gacgaaaacg gtgtatgcac ggccacggaa gattataagg
     1321 atgtagcgaa agtagctgct caaatcggga ttccttatta ctctgtgaat tttgaaaaag
     1381 aatattggga tcgtgtattt gaatatttcc tagccgaata ccgcgcagga cgtacaccta
     1441 acccagacgt gatgtgcaac aaagaaatca aattcaaagc attcttggat tatgcaatgg
     1501 atcttggcgc cgattatgta gctactggac attatgcgca agtcactcgc gatgaaaacg
     1561 gcgtggtcca catgcttcgt ggagtagaca ataataaaga tcaaacttac ttccttagcc
     1621 aattatcaca agaacagctt tcaaaaacga tgttccctct aggcggcatg gaaaaatcag
     1681 aagtacgggc aattgctgaa cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg
     1741 gaatctgctt tattggtgaa aagaacttca aacaattttt gagcaactat ctgccagcaa
     1801 aaaaaggaaa tatggtcact cttgatggtg aagtcaaagg acaacatgct ggcttgatgt
     1861 attatacgat tggtcaacgt caaggactag ggatcggtgg cggaggagat tcgcaagaac
     1921 cctggtttgt tgtaggaaaa gatctagcga caaacacatt atatgtgggg caagggttcc
     1981 atcatcctgc attgtatgcc acaagcttgg atgcaagtga aatccacttt acgacgaatg
     2041 aaccaatgcc aaaagaattc aagtgtacag cgaaattccg ttatcgtcag caagacgttc
     2101 ctgtgacagt tcgcttattg gatgataatc gtgcagaagt cgtattcgac gaacctgtac
     2161 gtgcaatcac tcctggacaa gcagttgtat tttatgatgg aatggaatgt ctgggtggtg
     2221 gtttgatcga ccacgcatat caagaaacaa aagttttaca atacgtttaa gcgatattaa
     2281 atgaatcaaa acagctatgg ctaacaaaag aaacaccttt tcttttggat agcatagctg
     2341 tttttttgca ataaaattaa tttttattca ttttatactt gaaatacttg tgtaaaaagt
     2401 tcataatatc caataatatg aataaagttg ctttttgtta agcaagagca gttgcaaaat
     2461 agaagggaat cttttgcaag tgatttgttc aattttataa gtgagggtga tggaaatgga
     2521 aaagaaatcg acgggtatta gcaaatgggg actggttgct ctggttgtca gttcgtcaat
     2581 cggaagcggt gtgtttggga tcacaagtga tctggctagt tctgcagctc cagggccagc
     2641 gatcctttca tggataatcg ttgggattgg tattttagct ttggtattat cgctgaatca
     2701 tttaggagaa aagcgtccag atctagatgg tggtattttc ggttacgcgg aagcttcatt
     2761 tggtaaatta ggtggcttca tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa
     2821 tgttgctttt gcaacgatgc tgatgagcgc catgggagaa tttttcccga tattcaaagg
     2881 tggacaaaat gttccttcta ttttatttgc aagtattttt atttggggtc tgactttatt
     2941 agtaaacaat ggagttgaag gagcgagcgt catcaataca atcgtgacga tctgtaaatt
     3001 agtgccatta ttcctatttt taatatttgt atttatcgca tttaaagtga atatattcac
     3061 tgctgatttt tggggtaacg tatctgataa tctagtgaat ggaacagggc agcaaggaac
     3121 gatctggttg cagattaaag gttgtttgat ggttatgatg tgggtctttg tagggatcga
     3181 aggcgcaagt gttttagcca accgcgcgaa gaaacgttca gaagcccagc aagcatcgat
     3241 cattggtctg ctttgtttat tgtttatcta tatccttgct tcgatcttgc catacggcgt
     3301 cttatctcaa gaagagttag caactatttc acaacctgct atggcaaata ttttaaaagg
     3361 aatcgtggga aattggggag cagcgctgat caatatcggc ttgattattt cgatcattgg
     3421 ttcttggttg tcttggacga tgctcccagc agaaacaacg atgttgatgg ctcgagacgg
     3481 tctgttgccg aaaggttggg gaaaaacgaa taaaaaaaat gccccaactt attcgttagt
     3541 tgttactgca atcttgacta atctattctt attgactttc ttagttactg attatgccta
     3601 tcaatttgcc tattctttat gtacagcggc aattttgatt tgttatctac ttgtcgggat
     3661 ctatcaagtc cagttttctt taaaagaagg aatcaaaaaa caagtagtga tcggtgggat
     3721 tgctgtagtt tttgaattga tgggcatcat tttagcagga ttttcttatg tccttttatg
     3781 tagtattgcc tatcttcctg gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca
     3841 tcaaattacg ggaaaagaaa aagtaatgat tgggctgatt tgtgtggccg cactgctttc
     3901 aatcgggttg cttgctgtag gctggatcaa gatataaggg ggaaagaaac atgaaaatcg
     3961 caaatttcgg tgtagaagaa tggctaaata aatgggaaaa gaaagcggta tatgatattg
     4021 cacaaagctc gatcgaagca ttgacattag acgaactcgt tggcttagat gggactagcg
     4081 taagtaaatt tttcgaaaaa cgcaaaaacg aaccattgga ctatggctgg atcgaaggat
     4141 cagatgtttt caaacaagaa gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct
     4201 tacaaacgaa tggagcgact ggtgctaatc ttttagctct atacgcgtta gtggaagcag
     4261 gagaccatgt tgtttcgatg cttcctactt atcagcaatt atatgacatc cccaaatctt
     4321 taggtgccgc tgttgacttt gttcatttga aggaagaaga agattggcag tttgatttag
     4381 aacagctaga agccttagtc aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca
     4441 atcctacagg cacattgcta gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta
     4501 cagtagatgc atatgtattg atagatgaag tatatgcgcc tttaacggat aaaggtgaat
     4561 tcttgtcgat cgtagatgtc tatgaaaaag gtatagcaac caattcccta tccaaaacct
     4621 actccattcc aggtattcgt attggctgga cagctactgg acctgagttg gcagaagttt
     4681 ttcgaaagta tcgagactac acgatgattt gcggcggagt cctgtcagat gatcttgctg
     4741 tacatgcatt gaagaataaa gaaaaaatat tggcgcgaaa caaaaagatc attacagaga
     4801 accttgtgat tttaaaacaa tgggtagcca atgaaccgga agttgaactt gtcgctccca
     4861 attacgtctc tacttctttc attaaattag ccatcgaaga agatgaccag acattttgta
     4921 tcaatttatt agaagaaaca ggagtactat tagtaccagg aagtgccttt gatttaccga
     4981 agcatgcccg actaggttac tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct
     5041 tatctgagtt cttgaaaaaa caggcctaaa caaaaacact gcaaggacag tctgtgacaa
     5101 ctgttcaagc agtgtttttc atttacgctt attgtctatg agaaggagag tgccctgaag
     5161 ctatggagac tcctgaagct ttttcttctg cagtttcttg ctgatggatc tcttgtgtat
     5221 tgtgtcgtac aagcaagaag ttgaccaaaa caagaagaga agtcgaaaga aagacgccgc
     5281 gataaccaaa tgcactggaa acactggagc cgatcatagg ccctactacg ttaccagttg
     5341 cttggaaaga ttggttataa ctgaagatcc gtccagcagc ttgatgaggc gagtattttg
     5401 tgatcaatgc ttggacagca gggagcagac aagcatcaga gataccaatc aaaaaacgta
     5461 aggcagctaa ttgccaaaca ttcgtgacga atgccattgg gatataaacc agaatcgcaa
     5521 aacccaaacc aatcgcaagt attctttcac tcccaatctt gtcacctaag cgaccaaatc
     5581 ttggagcggc tatcaaagtg gcaatcccag gtatcgaagc aatgattccg ctgatcaacg
     5641 taacattccc atgtccatgc agcaattggc gaatgtacag actgatgatt ggactaatgg
     5701 aattgttgga tgcttggatg atcatcgttg tgacgaacat accgatgacg acgtgtggat
     5761 atttcaagtc tttgaaaatc tgtttggcag aaaccatgtt ttcttttttc actgggacaa
     5821 atttttcatg gacgaaaaac agactcaaca agaagacgaa aaataagatg atccctgtaa
     5881 tgaaaaaggt agggcgatag ccaaacgctg aagcagagat cccgccaaga agcggtccaa
     5941 gtaatgttcc tgtaacagaa ccagttgcta aggttccaag aacttgacca cttttttctt
     6001 ttggcgtccc agttgcgaca agggcagtgg cattactgat ataaccagaa aaaatccctt
     6061 gaagcagacg caatgcgact aattgataca cactagtcac gcaacccatc aaagaaatga
     6121 cgattgccat tcctaatgaa gcacgcaaca gcatcaattt tcttcctttt tggtcggcta
     6181 attttcccca ccaaggagag ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc
     6241 cgctccaaaa gttcaattgt gacgtactat aattacctaa cgtatctatg taaagtgaca
     6301 taaaaggcat gactaaacta aatccgatac cagccataaa tgttccaaac cataagacaa
     6361 acaagttttg ttcccaagtt tctcttttcc taaataattt ttcctttatt cttgcagcca
     6421 attagactcc ttctttctga aaattttcat ctaca
//
LOCUS       DAJSMI010000146         6433 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJSMI010000146 DAJSMI010000000
VERSION     DAJSMI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.148"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            5..2377
                     /locus_tag="ON430_002298"
     CDS             5..2377
                     /locus_tag="ON430_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220198.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIREEIERFEKGESSFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQSELQAGIQAVANNIGIQIRDITESEIYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKLSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVQQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3835
                     /locus_tag="ON430_002299"
     CDS             2438..3835
                     /locus_tag="ON430_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR3220199.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEAKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLITELKPKHERQNF
                     LVRDNRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVHCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFDQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNGATRFTISA"
     gene            4446..6320
                     /gene="ltrA"
                     /locus_tag="ON430_002300"
     CDS             4446..6320
                     /gene="ltrA"
                     /locus_tag="ON430_002300"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3220200.1"
                     /translation="MKLTGSSKRIGLTIVRRNNLSVSTKLRYFEYYDMQKTFDWLYQR
                     SKHNAMTGVNLYDLIISERNILLAYRMIKANTGSKTAGVDGRTISDFKIKDRASFIRE
                     IRSTLQNFTPQGVKRVEIPKVNGKIRPLGIPTMRDRLIQQMFKQVLEPICEAKFYNHS
                     YGFRPNRSTHDAIARCNFLMNRSTFHYVVDVDIKAFFDNVNHKKLLNQLYTIGIKDRR
                     VLAIIGKMLKTPIEGLGVQGKGTPQGAILSPLLSNVVLNDLDQWVSSQWENIPTHFPY
                     KSNWVAKVQLRKSSKLKEVYIVRYADDFKIFAKNYKDAWKIFQAVKGYLNHHLKLEIS
                     PEKSKVTNLRKRSSEFLGFELRAIQNKGKYVASTHVSKKNKARIKQKVKEHLKILQKS
                     PSWQNVKAYNSYILGLHNYYRVATCVNIDFSKIAYSLSYTHFNRLKIVGKYEVPLRPP
                     PVYRKFYKNNLRTYKVAGLYLYPLADIQWKLKLAFSQITSDYTPQGRAKIYHNLNSSL
                     SNEIQKLQYSDHTGFSLEYEDNRISKYSMQNGKCAITGKFLTAQEVHCHHILPRRLGG
                     SDEFSNLIVVHEWIHILIHANHQSTIEQYKKLLNLTKTQIEKVNKYREKCNLTKIKYQ
                     "
BASE COUNT         2430 a         1025 c         1343 g         1635 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaatga acaaatcgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggttataaca agtacggcgg
      301 actagatatg acgatcgaaa atattccaag aatcagagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg tctttttcag ccgccacgat ccgaaaatat caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag taacgcaatg gaaaaaaaat ccgaccattt attttctgaa
      541 aggctatcga aaagtcgctt tagaactgaa tgaaaacggg gaatttgagg aatctaaaaa
      601 ataccaagcc acatcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctctgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggagag atcgtaacat tagaattgat
      781 cgatcaactc aaaacgttgg acaaccaata cgctgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggatatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatca ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaactca gttaaaacct gaagactatc cgaaaatctt
     1501 cccgaaccgt gtctttgatt ttcaattgga tcaaacaggg cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atatcgggat tcaaattcga gatattacgg agagtgaaat
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaact ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gcagcaggag cataaaaata ctgtagaaac ggcagaacaa aagaatattg tgcctgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggcc aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg cgtataaaac aagtaagaaa gactatctga
     2401 aaaatggata gtcttttttc ttttgggagg tttgaaaatg acagagaaaa aaatgagtct
     2461 cattgatcga tgcaagcaaa tggatattgt ggattttgca cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgtgact atcgcttaga agaccatgat tcgtttgttt tcgatcgaag
     2581 aaaacaacgt ttttattgga atagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttt atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag ccaaaacaga acgagtagaa aatcttcatt ttgagacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accacttaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaattgc cagagtggct gattgattat gcagaaaaag aaggattgat
     2881 tactgaacta aagccaaaac atgaacggca aaatttttta gttcgagaca atcgtttaga
     2941 tcatgcagtt gcctttttat ggaaagatcc acagacaaaa gagactgtgg gggcgagtta
     3001 tcaaggaacg tttattgatt atgaacgttt tggtgagaga ggaacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagttaaa
     3121 attttttgag agtagtattg atttacttag ctacgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatcgattg agatttgtgt
     3301 ggataatgat cgtgcaggac atattttcta cgaaaaggaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacattgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagtc aatgcgtctt ttgaccagca gctttctgtt aacgattcaa tagaagctat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaaggttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg ctcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagtg aaaaaggatt ggaacggtgc gacgcgtttc actataagtg cgtagcacga
     3841 aaagtgagaa tttacgtgaa gtaaattaca actgatgatt tgacagttgg aatgaaagcc
     3901 gtcatgttgg ctgaaacaac tgatttgata ctcctgcatg cgttcatgtg taacagtaca
     3961 tggaggtgtg aagccaggtg aagtcgacag aacaaaacct aagtaggatg cgtttctata
     4021 tggttgcgga tatgtcggaa tgctataaac aatctatggt gagaatgttt ctgttgagaa
     4081 actgacgaac ttgcgaatgt acgggtctat aagtcgactc tattcgaaag atagaggatt
     4141 gcgtgtgcaa agaacattac cgaaaagtaa gcgtcgttcc catgaaattc ggaatcgcaa
     4201 acaaagatgt gataaatgtt caggctcata gtaagcacct aaggattgat gtacagctag
     4261 aatcattgga acgcggaaag cgagaaacac ttaaacaaac gtctgttagc ggagtgttga
     4321 atataaggta agaactgaaa tttcttaatt ctcgtgagag tagaggcaca gtaccgttga
     4381 aattcatgaa aatgaataga gggatagcct caagacttgt tctttgaaat tttaataaca
     4441 gttaaatgaa actcacaggg tcgagtaaga ggataggact aacaatagtt aggagaaata
     4501 atctatcggt gagtacgaaa ttacgatatt ttgaatatta cgatatgcaa aagacttttg
     4561 attggctcta ccagcgcagt aaacataatg caatgacagg agttaatcta tacgatctca
     4621 tcatttcaga aagaaatatt ttattagcct atcggatgat aaaagcaaat acaggttcaa
     4681 aaactgctgg tgtagatgga cggacaattt ctgattttaa aattaaagat agagcgagtt
     4741 ttatcagaga aatcagatca accttgcaaa acttcacacc tcaaggtgtg aaaagagtag
     4801 aaattcctaa agtgaatgga aaaatacgac ctttaggtat acctactatg cgagatcgtt
     4861 tgatacaaca gatgtttaag caagtactag aaccgatttg tgaagcaaaa ttttacaatc
     4921 attcatatgg gtttcgtccc aacagatcga ctcatgatgc catcgcaagg tgtaattttt
     4981 tgatgaatcg atcgactttt cactatgtcg tagatgtaga tattaaagct ttctttgata
     5041 atgtgaacca caaaaagctc ctaaatcaac tttacactat tggaataaaa gataggagag
     5101 ttctcgccat tataggaaaa atgcttaaaa ctcctattga aggtttggga gtacaaggaa
     5161 agggaacacc tcaaggtgct attctttctc cccttctttc caatgtagta ttgaatgatt
     5221 tagatcagtg ggtaagtagt caatgggaaa atattcctac acattttcct tacaagagta
     5281 attgggtagc gaaagtacaa cttcgaaaga gttctaaact caaagaggtg tatatcgtac
     5341 gttatgcgga tgatttcaaa atctttgcga aaaattataa agatgcttgg aaaatatttc
     5401 aagcagttaa aggatatttg aatcaccacc tcaagctaga gatttcaccg gagaaatcca
     5461 aagtcactaa tctgaggaaa cgaagttcag aatttttagg cttcgaattg agagcaatac
     5521 aaaataaagg gaaatatgtg gcgagcacac acgtttccaa gaaaaataag gcaagaatta
     5581 aacaaaaagt gaaagaacac ttaaaaatac tacagaaaag tccgagttgg cagaatgtaa
     5641 aagcttataa ctcttatatt ctaggactac acaactatta cagagtagcc acttgtgtga
     5701 atattgactt ttcaaaaatt gcctattctc tctcttatac tcacttcaat cgtttaaaaa
     5761 ttgtagggaa atatgaagtt cccttgcggc cacctcccgt atatcgtaaa ttctataaaa
     5821 acaatttgag aacttacaaa gtagctggtt tataccttta tcctttggca gatattcaat
     5881 ggaaacttaa acttgcattc agtcaaataa ccagtgatta taccccacaa ggaagggcaa
     5941 agatatatca taatcttaat tcttcacttt ctaatgaaat tcaaaaactt caatattcgg
     6001 atcatacagg tttttcctta gaatatgaag ataatagaat ttcaaaatac tctatgcaaa
     6061 atgggaagtg tgccattaca ggaaaatttt taacggcaca agaagttcac tgtcatcata
     6121 ttttgcctag aagattagga ggtagcgatg aattttctaa tttgattgtg gttcatgaat
     6181 ggattcatat tcttattcat gcgaaccatc aatcaacgat tgaacagtat aaaaagcttc
     6241 ttaatctaac aaaaacacag attgagaaag tgaataaata tcgtgaaaaa tgtaatttaa
     6301 ctaaaattaa atatcaataa caagttggaa cgccgtatgc gttgaaagat gcacgtacgg
     6361 tgtggagtgg gggaaaacct agagagacct ttaatagcta agaaaataaa gctcaaaggg
     6421 ttacctatca cta
//
LOCUS       DAJSMI010000147         6427 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJSMI010000147 DAJSMI010000000
VERSION     DAJSMI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.149"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(53..787)
                     /locus_tag="ON430_002301"
     CDS             complement(53..787)
                     /locus_tag="ON430_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR3220201.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ON430_002302"
     CDS             1114..1524
                     /locus_tag="ON430_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR3220202.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ON430_002303"
     CDS             1517..2218
                     /locus_tag="ON430_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCR3220203.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ON430_002304"
     CDS             2218..3804
                     /locus_tag="ON430_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3220204.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ON430_002305"
     CDS             3823..4044
                     /locus_tag="ON430_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCR3220205.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ON430_002306"
     CDS             4041..4802
                     /locus_tag="ON430_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCR3220206.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ON430_002307"
     CDS             5182..5691
                     /locus_tag="ON430_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR3220207.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ON430_002308"
     CDS             complement(5766..6305)
                     /locus_tag="ON430_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCR3220208.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
BASE COUNT         2008 a         1178 c         1302 g         1939 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tcttttt
//
LOCUS       DAJSMI010000148         6307 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJSMI010000148 DAJSMI010000000
VERSION     DAJSMI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.150"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(85..1215)
                     /locus_tag="ON430_002309"
     CDS             complement(85..1215)
                     /locus_tag="ON430_002309"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR3220209.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1275..1502)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1864..3066)
                     /locus_tag="ON430_002310"
     CDS             complement(1864..3066)
                     /locus_tag="ON430_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3220210.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3126..3329)
                     /locus_tag="ON430_002311"
     CDS             complement(3126..3329)
                     /locus_tag="ON430_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCR3220211.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3471..4703)
                     /locus_tag="ON430_002312"
                     /pseudo
     CDS             complement(3471..4703)
                     /locus_tag="ON430_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4866..5678
                     /locus_tag="ON430_002313"
     CDS             4866..5678
                     /locus_tag="ON430_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3220212.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1869 a         1299 c          996 g         2143 t
ORIGIN      
        1 aaaggtaaat aaaaaagagt ggatttctcc actccatgaa aagccgactt tttgggacgc
       61 ttttttttgc caagcctcac atttttatag ccaaacttct tttgcgactt gctgcaataa
      121 ttggattttc ttccattgtt cttcttctgt taaatggttt ccttcttctg ttgaagcaaa
      181 cccacattga ggactgatac ttagattttc caatggtaca aattgttgtg cttcttttat
      241 tcgttctttt attttctccg tatcttccaa atctggaaac ttagaagtaa ctaatcctaa
      301 aacaacttta ctttctttgt tactccaatg tcttaaagga gtaaaatctc ctgctcggtc
      361 agaatcgtat tccaaaaaga aaccatcata atttgttgaa aacaactctt ctgcaatggg
      421 ttcatatcca ccggaaaaca gccaagaaga agcgtgattc ccgcggcaga tatgtgttgt
      481 gatcgtcaaa tcttctggtt tgtcttccag tgcgttattg ataatctctg tacaaatatg
      541 ggccatttct ttcggatcaa ttcctttttg aagaagtgct tctcttttag tttgatcagc
      601 tagaaacgcc caatttgtat catcgaattg caggtaacgg caaccttttt cataaaaagc
      661 atgaatcgct gctttgtaag tagaggacaa gtcctcataa aaatcagata gatcaggata
      721 aatctccttg atttttgaag aaccatcatt agataggatc tcttgtcgta aaatcatcgt
      781 gggactaggg atcgttgctt ttgcaacaaa agtatcatct gtttccacta cttctttcag
      841 aaaagcaaaa tcttttaaaa atgggtgatt tggattgaaa gataatttat ctactacccg
      901 aatattataa gctggtgctg cttttccgtg gaatgtttga ttatatccag tttctggaac
      961 atagccttcg ataccattta gttcttctaa aaaatcaaga tgccaaaatc cacgccgaaa
     1021 ttcaccatct gtaattcctt tgatgccgac tgctttttgg tcttccacta atttttttat
     1081 ttcctggtct tcgattgcac gaagttcttc ttttgaaagt aagccttctt gaactttttt
     1141 tcttgcttct ttgatcgatt gcgggcgtaa gaaacttccg acatgatcta ctgtaaatgg
     1201 tctatttttg ctcatttcac tcactctttt ctttgttttt tgtaaaaaaa aatcctttta
     1261 ctagcaaata ctagtaaaag gacgattata ctcgtggtac cacctttttt cagaaaaaac
     1321 attctttctc tcagcctgac aataaaatca gtggtcttgg taacgaaaga ctgttcgttt
     1381 ttgcctacct ttcgacaaaa aatcctctaa ggccattttc gacagtcaac ttctagcttt
     1441 ttacaccaac caaagcctct cttgaaccat tttctgtcta ctttccttct catcggatac
     1501 tattagaata aaattagaat agcatgtttg aaagaaaagt caatagggaa taagaacaaa
     1561 caaaaactaa tttgttattc ttagtaattt gtttgtggaa tacaataaag cttttctaat
     1621 atgataattg ttcagtcgtc ctttcatctt agatcacttc cttaaaatga tcatacccgt
     1681 ttcaaccaca taaaaaagga cttaattttg aaaaatttcc catcaaaact aagtcctttt
     1741 tattatagtt atgcaaattt gcttattttc tcgctatttc cgatacatct tgaacaggag
     1801 caattcgact gaaaacactg gtgcaaaagc atttatttgt cttatggtag aaccgtggaa
     1861 aactcagact ggtaataaat tgtcattttt tgaaagaata gcgccctctt gaacattatc
     1921 aatcgcactt accacttgtt gatctgactt tgcatataag tgaccgtaag ttcctaaagt
     1981 gatttgaata ttcgcatggc ccaatcgttc ttgaatagat agcggttgaa tattctgctc
     2041 aattagaaat gcaacatgag aatgtcgtag cgcgtgaagc ttaatcggtt tgacacccgc
     2101 ttttttagca gcttctttta accagttcgc aaaagaataa ggtggcgtaa atgttccgtc
     2161 caactgagca acatattcaa aatttccaat acgtttttgc aagtcatacc actgtctgag
     2221 ttttaaggaa agcttcttcc ccaacccaat cgttcgataa gcacatttag ttttgggagt
     2281 cgaaaagtac caatcatctt ttgtattata aatcaatgtc ttctcaatcg ttatcgtgtt
     2341 tttctcaaaa tctacattcg accaagacaa agcttcaagt tctccaatcc gaatgccagt
     2401 ataaaagagc atttcataca caatcttccg atggtgggtt tgaatcttat catccttcaa
     2461 ttcattcatc actttttgaa actcactgtg ttcccaaaac tcaactttct tattctcaga
     2521 aggaagcgaa gaaacatctt caacgggatt tttagcaatc accccctctt taacagcccg
     2581 atccaataca atttttaggt gccctcgaat ccgattgata taagattttg ataacgtttc
     2641 gccattaact tttttccgtg aattacgtga ctctgtctgg cttagttcaa ataaccaatc
     2701 ctcaatatcg tgacttgtaa tatctcgaac acgaaaatga tagaaaaact gcatacgctt
     2761 ttcgaaaatg aattgcgcat tctcatatgt ttgacttttg acctgtctct cgtaccattt
     2821 tacaaaccta tccttgtaaa actcttcgaa agtcatttga ttggtagaac acagttgatt
     2881 aggaattgtc ttgttaaaat catgtgtttc catgtacttg tcataagcaa ttttggcatc
     2941 tttttgtgaa gcataacctc gtttagtctt ctgaaccttt cttccatttt catctatacc
     3001 tagatacgct cggaagaacc atttttttgt ctttttatct tggtaaatat ttttaatttt
     3061 tggcatcgtt ccattcctcc ctgtacttga gaaaagctac tttaatagta ccgcgcttgt
     3121 actgtttatt ccgcaacttg aatgtgaaaa agatgttcaa ttactcggga tggcaccaca
     3181 ccaacttggc gattattata aaaatcaata ccttcaatcc ttgcaagata tgccttgctc
     3241 tctttaatca ttcgtgcagc ttgactaggt ttaaagccca tctctatcaa aattgcacga
     3301 tttaccatga aatcacattt tgtttccatt ctctcacctc ctgattcttt actcctttta
     3361 aaataatcct ctttataatc tattgtttct cattcgatgg ttgtgttata ctcgtcttgt
     3421 cgaggcggag tacgttgcac aacttgttgt gtaagcagta ctttgctttt ttagtagctt
     3481 gttttctcat ttgatatact ttgtaatttt cgttcataat actcaacctc ctcctgataa
     3541 tactccgggt ctgccttcat attttggaga agtttgatct gtcttggatt catttctcct
     3601 tgttcaataa tcaaaggcaa caaggaagaa tgaaacgttt gatcgacctg tgcaataaaa
     3661 actaatgatg gtgaaacacc atgaattaac caactcatag ttttctcaaa actttgagga
     3721 attgtatcta aagaaatggt gattgcgcca taattgctaa taaaggctgc ccatttggca
     3781 tcaacttttg cacctagatc atttggatca cggtcataaa aacaaattgc tccattaagt
     3841 aactcaaaga acagtttggt taaatcatgg gtttttgaaa taatctttgc caagctttgc
     3901 gctttctctt ttcgataccg tagttcaaag cgattcttta cttttgcctc ttctaaagga
     3961 attcctcgtc gcttccgttg ttcatggtcc ttctgataaa agcaaaaacg acactgactc
     4021 ttctttgagc ctaaatacaa ggtctcacca tttgattcat tgtttatgat atccgtaccg
     4081 ccatgagatt cactcacttg aaaagtggtc cacacatggc ctagtattac cttatgctta
     4141 agttctggaa tgctgagact ccccacaaaa tcatctaaag taaaatcaat ccgtgtaaag
     4201 ttcccctgat aatctaatac cttacgaaaa aactgttgcc aatttgtttt tcgtgttttt
     4261 agccatcttg ctaagtgttt acagccttga cctgaaaact ccatcacagt tcctttaacc
     4321 gcatcgtcaa tggaataccg aatcgtgatt acttgattcc acacgtattg gccaatatag
     4381 ccctttggcg cataatccag ttcctcaaaa cgttcaatgg gcatttcaat aatctcttca
     4441 attagcttta aatgttgatt cgagaaaaag tgaatggtta gataatcaat caaaacacgg
     4501 ttaactggaa cgacctcttc ttgcaaacta tccaacacaa gattcaattt ttcttcactt
     4561 aattgtctcg ttcctttttc tactttcgat aataactatc tcgaaatccc cactttttct
     4621 gctaaatatc gttgtgtcca ccccctttct tctcgagaaa ctcgaatggc ttcaccgaga
     4681 tcatgttgat taacttggtt catcgacaaa cctccttctg ctatttactt gtcaaagaac
     4741 ctattgaact tattttaagt acatttttat tgtacgcctg tatttatttt aagtcaatat
     4801 gttttgactt tttttaagta cacacgtata ctaaatttaa gaacttatcc tgaggaggag
     4861 ttttcatgga gatagacaag caagcagtcg gcaatcgaat tcggcagatt cgccaagaat
     4921 taaagttgag tatggagaaa tttggcaaac taattggtga tttaccgcga agtacagtca
     4981 ataactggga acgtgggatc aaccttccaa aaaccgaaac ccttcaccaa atcgcagaga
     5041 taggtcatgt caccaatgaa tatcttttgt atggtgatca agagaatcaa tacattttag
     5101 aaatgctcca aaaaaaggca ggtaagcttg atcctaagat tgaaggtctc atagtagacg
     5161 aaatcaaaca cgctgacctt gttagtgaaa aagaaatgga tcgaatgatt gaatttttta
     5221 ttacaaatct cattccacca actgaacaag accagtttac tttccaatgt atcgatgaaa
     5281 aaaagcagct ttatcttggt tcaactaatt ttggtaagga agcccagctc tatttgcaac
     5341 atgataatca aaatcatatt ctgcacatga tgccctttac cttctccaac tttagtgtgg
     5401 atcgcatact cgtctattta gcaaatcaaa aatcctattc ctattttggg aaacatttac
     5461 ccaaggaact aggtgagaaa gccatccttc tatactcaat taatcaattg accagcgatg
     5521 tgcgaattgc cccgttggtc tattcagaag aaaccgcttc ttatcagtac acagaggaca
     5581 atcaatatct actagaacag caatatcttt atctaccatt cgtgatggaa gtcgagaaag
     5641 aacgcctgtt aaatgcagcg tatccatcat caaaatgagg tgacaaaatt gataagcacg
     5701 tgacaaagaa aatactgcta ggacactccc caatgggtag ttgtcacagt ttcctgttct
     5761 ttgttagccc cctgttagaa ccgggggctt tattcctgca gtccggctac gccttcctgc
     5821 aatagacaaa actccagatc gggtaattcg cttgtggcga aacccgacct ggagtttgtc
     5881 tctgtatttt gaaccctaaa aattaatcaa acagactgat attctcataa tcagcgcgaa
     5941 ataagccatt aagtacagtt ttcctaccta tttttgataa cccgctggtt agttaatatt
     6001 ccttttccga gagagataaa tgattggtta aatctatata catctcaacc tttaaaaatg
     6061 ttttcgaaat acttcatcag tacatgtcat acaaattacc agtgcattct caaattctag
     6121 ttctttaatt gatttcagtt gaggagcagt tacttttggc ggaaatttcc atttttgtcc
     6181 aaacctcttc ccccgatgaa atttctttgt tgcaattatt gcaatgaaaa atttttgttt
     6241 caaaaatata cataattact tcctccaata aaaactatct acctctcaaa ttatttttct
     6301 ctaataa
//
LOCUS       DAJSMI010000149         6197 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJSMI010000149 DAJSMI010000000
VERSION     DAJSMI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.151"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..443
                     /locus_tag="ON430_002314"
     CDS             <1..443
                     /locus_tag="ON430_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220213.1"
                     /translation="TSELLKLYGIQRDNRASDFGKIIYTEEKEFNKKIFTLLEGKQGV
                     SPYDVFPDFDALYKKYMFAEYYIVYNYFLENIASNESYGDLMDAYYSYLKKYAVVADS
                     EKLLLYWPKYVQYNVKFENELDFYAKLDSKKWMDIYANYTPFSQ"
     gene            469..1554
                     /locus_tag="ON430_002315"
     CDS             469..1554
                     /locus_tag="ON430_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HCR3220214.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            1577..2392
                     /locus_tag="ON430_002316"
     CDS             1577..2392
                     /locus_tag="ON430_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220215.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
     gene            2376..4094
                     /locus_tag="ON430_002317"
     CDS             2376..4094
                     /locus_tag="ON430_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HCR3220216.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERMSVNAFIDYCPDLVI
                     TIGKHVWSFVKYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGV
                     NSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDF
                     VSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIP
                     DNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYITADS
                     KESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVITPKMYFRKALDIIK
                     KHLHIK"
     gene            4120..4956
                     /locus_tag="ON430_002318"
     CDS             4120..4956
                     /locus_tag="ON430_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3220217.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            4961..>6197
                     /locus_tag="ON430_002319"
     CDS             4961..>6197
                     /locus_tag="ON430_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR3220218.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILR"
BASE COUNT         2027 a          822 c         1233 g         2115 t
ORIGIN      
        1 aaactagtga attattaaaa ttatatggta ttcaaagaga taaccgagca tcagactttg
       61 ggaaaattat ttatactgag gaaaaagagt ttaataaaaa gatatttaca ctactagaag
      121 gaaaacaagg tgtatctcca tatgatgttt ttccagattt tgatgcacta tataaaaaat
      181 atatgtttgc agaatactat attgtgtata attatttcct ggaaaatatt gcaagtaatg
      241 aatcttatgg tgacttaatg gatgcctatt atagttattt aaagaaatat gcagttgttg
      301 ctgattcaga aaaattgtta ctatattggc cgaaatatgt tcaatataat gttaagttcg
      361 aaaatgaact tgatttctat gcgaagttgg attcaaaaaa atggatggat atttatgcta
      421 attatacacc ttttagtcag tgattatatg gaaaggatga ggtgaataat gaaaatcgga
      481 atagtgacta ttgttaatgg ggcaagtttt ggtaacagat tacaaaatta tgcgactcaa
      541 aaagtgttgg aaaataacgg ctatacagtt gagacaatta ataattgttt tgacaaagaa
      601 agtaccctat ccattgttat caaaaacgct atttatacat tatttaagtt tataccgcat
      661 ttcaaaaagt caaaaagtat ctggaaaaga atgtatacat tttatcaatg ggacaagcag
      721 tatatagatt attctcatcg taaaattgat gcatctaaaa aaaacagctt tgtgagtata
      781 aagaatgatt atgactattt cttggttggt agcgatcaaa tttggaatcc aaaatatgca
      841 gccaataagg gatttgaact tttatcattt gttccaaatg aaaaaagagt ttcattatca
      901 accagtttgg gagttccatc tataccaagt gatttagaaa aattatatgt gacagagtgg
      961 aataagttta aatcaatatc ggtacgtgaa aatcaagcgg caaaaataat tgaggacttg
     1021 acaggcacaa ctgcggctgt tcacattgac ccaactttaa tgttggagcg ttcggaatgg
     1081 gaagcctttc aaaaaaaacc agacaatatt gatgaaaatt acatcttgtc gtttttctta
     1141 actaatccgc ctaaggatac tgttgatgta ttagataagg tcgctaaaga tttgaaaata
     1201 ggtgttctca ccttagatgc gtcttgggaa tattttcaag atattactcc gaatcatttt
     1261 gtttatttga tttcacatgc aagaatgatt tatacagatt cattccactg cacagttttt
     1321 tcattgctat ttggaaaacc ggttgtggta tttgaccgtt ttgatagtga aaaaatgaat
     1381 tctaggttaa gtacattgtt aaataaagta aataaagcac atttttgggt aggaaaaaaa
     1441 gctgatgaag caatgattcg tgcaatagca tctgaacctg aatatgacat agataagctg
     1501 atggtatcag agagggaatg tgtaaaagct tatcttaaag agtatttaca gtaaaatttt
     1561 gaaatgggat gaaaatatgg agcatttaag tgattgtttt ggtaaaatga agattgttac
     1621 gacttcatgc gcaagagaat attttaagga tgatttggat aaatcagagt attcatgttt
     1681 tgttgattct cgagcagcta tgtttttcgc aatgggacaa gctttagcta aaagaaggaa
     1741 tgtagttgta atgcttgctg atgaattgac atcgtgttat acggcactaa cagaatgtgc
     1801 atttcaacaa gttgatttaa ttgtgtttgc ttttcagaca gtcagacatg aaattgattt
     1861 agcatctttg aagaattgtg gttgcaatct aattaactgt agcgcccaag acgatattga
     1921 tagagttgtg tccaacacta aggggttaaa tcagacaaca gtaattaaaa tcaatatgaa
     1981 ttgtgagtcg gataaagatc atatatgtga gtatatagac ctgccagaca tacctgtttt
     2041 tttccacggt agcttatact cacctgcaag taaaaagaaa tatgagcagt atggacaggt
     2101 atcgaagtat ttaggctatc tcgtgggggg aaaagatgca atactcataa tttctcacaa
     2161 tgagttttta aacgatatca gtgtatggat taattattat tcttttggag aaattcaacc
     2221 caaaattatt atatttgaca gtccgaatga ctatcaatat aaatggctgt gtgacttaga
     2281 atgcagtgta gctcattcaa ccaaccgatt tttgtctgag gatttagcct tattgcaaaa
     2341 taacggaatt tccattgtgt taagaggtga taattatgat atcgtcagtt aaaaatgttc
     2401 aaattttagt atctttatta aaacagcata atattaaaga tatagttatc tcgccaggta
     2461 gtagagattt tcaattggtg catagtgtag aaacagacag tttttttaga acctatacgg
     2521 ttgtagatga gcgcagtgct gccttttttg ctaatggact ttctatggcg attgataagc
     2581 ccgtgtgtgt atgttgtact tcttcaactg cctcttcaaa ttatatggcg gcagctaaag
     2641 atgcttttgc taatggcatc caacttgtac ttcttactgc tgatagagat taccgccgct
     2701 tatatcagat ggaagatcag atgattgatc aggtagatat gtatggggct tatgtgcgtt
     2761 ctaacgttga tattcctatt gttaaggatt ctgatgatga atggtatgct accagaaaaa
     2821 taaatgaagc aatattggag ttaaatcatg gcgttaaggg tcccgtgcag attaattatc
     2881 aattaatgga tttaagtgcg ccgtttgttg agaccatgcc agagtatcgt aaaattacaa
     2941 gatatcaaga ggatgattta ctgtcatcag tagagatttt taaacagaaa ctattatcaa
     3001 aaaagagaat aatggtttta tgtggacagc aggaagaaaa taatttaagt gccttattgg
     3061 aagagttctt tattaaatat aatacaatta taatcaagga taactattct aatatacacg
     3121 gtgaacactt tattaagact gttctagtaa cagaaagaat gagtgtaaat gcatttattg
     3181 attattgccc agatttggta ataactattg gtaaacatgt atggtctttt gtgaaatata
     3241 ctcttagaga taaaaagctt aaatttgagc attggcggat atctgaggat ggtaagattg
     3301 ttgatccgtt caacaagcta acatctgtct ttcagtgctc agcggaattt tttatggaga
     3361 ctgtcactgc tggtgtgaat agcaataata atcttgagta ttataatcta tggaagacaa
     3421 gattatcaaa agtttgctat ccagagttag tctatagcaa tttttatgca atttcagaac
     3481 tagtaaaaaa aattccagat agtgcattag ttcattctag tattttaaat gcggctagat
     3541 tgtatgactt tgtatctgat gataaagcat tactcagttt tgctaattta ggggctgatg
     3601 gtattgatgg ttgtttacca acatttttag gtcaggcaga agcagataaa actcgacctg
     3661 cgtatttaat ttcaggagac ttgagtttct tttatgctat gaatagttta tgtggtgaca
     3721 ttccagataa tgcacatatt ttccttatta acaatcattc tggaagtgaa ttccatacaa
     3781 attttggtaa gttttacaac ggatcattaa gtgttgataa gcatatagct gcaggacata
     3841 tttcaaacgc aaaggattgg gcattagaaa ataatataaa atacattact gcggattcta
     3901 aggaaagtct caaatcgggg ttggatgtat ttattaatac atttgacagt cctgttttat
     3961 ttgaggtctt tacagatccg gatacggata cgtctgttct ggaatctttt tatagtataa
     4021 ataaggtaat cacaccaaaa atgtatttta gaaaggcgtt agatattata aaaaagcatt
     4081 tacacattaa gtagatgttt attgcgaggt gaaacaaata tggcaataaa atcttatatt
     4141 aaacggttat tgctttatat ccgtcatgga gatcccaaaa taaatttaga tgtgagttat
     4201 ttatctgaag gtgcaatgct tgctggtaag actgtgataa ttacgggtgg tgcaaaagga
     4261 cttggttttg caattgcaaa atcatttata gctcaaggcg caagcgttat tattacagga
     4321 agagacatgg attcgttgaa taatgctaaa tgtcagcttg gagatatgtg ttctatcagg
     4381 gcgtttgata taactgatgc taaaatgtat cgaacgtttt ttgaagatgc atacgagacc
     4441 tttggacgta ttgatatact gcttaataat gctgggatct cgttacacga agatggattt
     4501 atgagtgtta ctgagaaaac ttgggataag caaatggata caaatttgaa aggtgtttat
     4561 ttctttacac aggaatttat taagtttgtt aaagaaaaga atcaaatgca agcaaatatt
     4621 attatgatgt cctcagaaag aggcttgtgt ggtgatagta ttccgtatgg attatcaaag
     4681 gctgcaatta actctttagt tgtaggcttg tctaagaaat atatttctga gggaattagg
     4741 gttaatggta ttgctcctgg agtaactgca accgctatga ctggtatttc tgaaaagggt
     4801 aatttgtata atcctgcaac gatcggttat agatattttc tcccagaaga agttgctcaa
     4861 gttgctttgt ttttggcaag tagtctttca aactgtatca gtggtgaaat catagcctgt
     4921 gaccaggggc aatatttaag tcgaaattgg gggtaataac ttgggaatat tattaaaaaa
     4981 atataatagc cttagtgagc ctgcaaaagc atctatttgg tttacacttt gtaatgtgtt
     5041 gcaaaaagga attctatttt tgactacacc tatttttaca agaatgcttt caactgcaga
     5101 atatggtaag tatacgattt ttatatcgtg gtattctatt atagcaattt tcacaacgtt
     5161 gaatttatct gcaggtgtct ataataaagg actaattaaa tacgagaata aatatgcatt
     5221 aacctcgtca tttttaggat tatcgtcatt tatttccctt tgtgcattga tgctatacct
     5281 tccatttaga acggttataa atgggctact ggatttatcg acggagtttg tcatattgat
     5341 tcttattgaa tcaatatttg agccggcttt tttgctttgg tcagttaagg aacgatttga
     5401 ttacaagtat caaaaactag tactcatcac agttcttatg gctgttttaa atccaggtct
     5461 cggtattata ttaatcttat catttgggga gtatgctatt tggagagtac gagcttatgt
     5521 cgtggtaaat gttgtagtgg ggatgtttat tggcatagga atactggcta agggtaagaa
     5581 attatttgat aaagagtact ggacatatgc gctcagattt aatttgccat taataccaca
     5641 ttatttatca atgacattgc tgaatcaggt tgatcgcctt atgataaata gtatggttgg
     5701 agctagcgag gctgcgattt atagtatcgc ttacactata tctagtatga tgaatatcat
     5761 aacaacagct ataaacggtt cttttattcc ttttacatac aaagcaatta aagatggcgt
     5821 gtatgaaaaa attaaatccg ttgctaatca actattagtg ttgatttttg tcttgtgttc
     5881 gtttactatg ctgttcgggc cagaattgat ttgggttgtc ggaggagaaa aatatatgga
     5941 tgctgtttgg attattcctc cagttgcggc atcagtattt ttcatgtttt tatatccgct
     6001 ttttggcaat attgaatttt actatgaaaa gacaaactac gtaatgtttg catcttgtgt
     6061 gggctctatc gcaaaaatta tttcaaacta tgttagtatt ccccgttttg gatatatttc
     6121 ggctggttac acgacattga tttgctatat gttgttttcc tttgcccact acatgtttta
     6181 taaaaaaatt cttagag
//
LOCUS       DAJSMI010000150         6131 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJSMI010000150 DAJSMI010000000
VERSION     DAJSMI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.152"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(343..828)
                     /locus_tag="ON430_002320"
     CDS             complement(343..828)
                     /locus_tag="ON430_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220219.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(967..1182)
                     /locus_tag="ON430_002321"
     CDS             complement(967..1182)
                     /locus_tag="ON430_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCR3220220.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1406..2206
                     /locus_tag="ON430_002322"
     CDS             1406..2206
                     /locus_tag="ON430_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3220221.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2225..4093
                     /locus_tag="ON430_002323"
     CDS             2225..4093
                     /locus_tag="ON430_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR3220222.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4086..4577
                     /locus_tag="ON430_002324"
     CDS             4086..4577
                     /locus_tag="ON430_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR3220223.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4565..5119
                     /gene="srlA"
                     /locus_tag="ON430_002325"
     CDS             4565..5119
                     /gene="srlA"
                     /locus_tag="ON430_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR3220224.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5137..>6131
                     /locus_tag="ON430_002326"
     CDS             5137..>6131
                     /locus_tag="ON430_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR3220225.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2169 a          972 c         1226 g         1764 t
ORIGIN      
        1 atacaaaggc tatctttcta atttgaaaaa gggatcatta aaataataag aagaaaatat
       61 gtcaaaaggt aaaaaagtaa attcttctat ccaaatatag aaaaattgaa tctagaaaac
      121 cgcccgacaa taaatttcat taactcagtt gaccgaaaat gaagtagata ccaaaaattt
      181 atcaagagag caagtagaga agtgagtata ttcataagag aaatagctgt aacgaattat
      241 taggttaatg caaatgctta tttatactcc gtataacttt ctcagaaaaa atgataccaa
      301 taatgacttg ctagtactgt aaatccgtat aatatcatct aatcaaaatg gtgttccatt
      361 tgcatctgtc atcgtaatcg atccttcggg tgacactcga taaaaaccaa cgcttccaga
      421 tcctccttgg gaacggattt gtttgctgga agctgtgaac aaataatctc gatcaatttt
      481 ttgataaaag ctaataacaa tatcgtcact ttgtaattcg gaacggtggg caataagaaa
      541 tagaagtgct ttgtcagagt tttctaatgg aagagaaggt acagtgcttt cttgcatttc
      601 agatgttgaa gaaatgctaa acgactgaat acttgattgg ctatgttgcg aatctacatt
      661 accagtagaa gtattagtaa tggaagtagt agaagtactt ttagttgaac caacctcagt
      721 tgttgaaatg gctgctgaag attctttaac agaattcttt gttttatatc cacaggcaga
      781 aaaaagtatt acagttccca acaagcctgc gtaaatccct ttatgcatga tagctcctcc
      841 taaaatagat tatctttatt cagtatatca cttaaacaaa gaaaagcaga tggtttaaaa
      901 tgaaaaaacc ttcccaataa agggaaggtt catgaagata ataacttttt taataattat
      961 agctttctaa tatctttcat caacttttct agtaatcatt gggaaaaatt tcaagcagta
     1021 gatcgcacaa atacctacga cgatataaat gatcttcgca aaaatagttg ttgaaccacc
     1081 agcaatcata gctactaaat caagttcgaa caagcctacc aacagccagt tcaaccctcc
     1141 cacaatcaat aaagttaatg caatagcatc taaagttttc attagaattc tcctttctta
     1201 aatgataaaa caattatata tcaaaaagag aaacattgct tatataacgc tcaggaattt
     1261 tagcaatcta tgcaaaaaga tgaaaatttt ttttgctctt ccattagtac acatttttta
     1321 gctgaagctg taaatttcat gtcttaattt tttttagtga gcttttatac tggatttgta
     1381 agaaaaaaga aggagaggat cacatatgtc ggattggttg gatattgcag gaaaagtagt
     1441 aattgtaact ggaggttcct caggaattgg aagaagtatc gtcgaaaatt tgttgaagca
     1501 gaatgcacaa gtagcgaatt ttgatgtaac tgaatgccgt atacagcatg aaaacctact
     1561 atctttaaaa gtcgatgtgt cttctaaaac ggacatagaa gaagggatat ataaagtaat
     1621 gaaacatttc aagacgatcg atggtctagt caataatgca ggaataaata ttccttctct
     1681 tctaatagat agaaatcatc caaaaagcaa gtatgaatta tcagaacaag tttttgataa
     1741 gatgatcgct gtcaatcaaa aaagtgtata tttgatgagc caagcagttg gaagaatctt
     1801 ggtgcaaaag ggatcaggag tgatcgtgaa tctttcttct gaatcaggat tggaaggatc
     1861 agaaggacag agctgttatg ctgctacaaa agcagcgatg aacagtttta ctcgttcttg
     1921 ggcaaaagag ttaggaaaga ggaatgttcg tgtggtaggt gttgcaccag ggatactgga
     1981 agaaaccggt ttgagaacac aagaatatga agaagctctt tcttatacga gaggtataag
     2041 cgtcgaacag ctgagaaacg gttacagtcg aacaagtacg atccctctag gaaggagtgg
     2101 taagctgcaa gaagtggcgg atctagtctg ctattatctt tcagaaagat caagttatat
     2161 cactggagtc acgactaata tttcaggagg taaaacaaga ggataaagga taggggggac
     2221 gcacgtgacg ctagtaaaca gatggtacca attattgaaa ttactagtca ctcacaagaa
     2281 agtttcaatc caggaagtgc aaaaagaaat caaaaccagt aatcaaacca taaaaaaaga
     2341 tattggggag ttgaatgaag aaattgccgg tattgcaaaa atcatccaaa aaaacaaaca
     2401 ttttgaactt gaaatttatg attttgattt ttttgatgag atcatgcaag gaaaattgaa
     2461 aacagccagt gatttcaatt cggcatccaa aagaatcgct tatttactaa ataggctgat
     2521 cgaaacagag gatttcttac gtatggatga tttgtctgaa gaagtcggtg tcagcagagg
     2581 aacagtggtc aaggatctaa agacattaaa agagatgatc aatgattttg atgtaaagat
     2641 tacaggcaca ccaaataaag gactaagaat aataggagat gagctagaat tacgactact
     2701 tttattttat tttgtttctc cctacttttc agagacgaaa acagaacagt ttctagaaga
     2761 aaaacaagca atcaaacatt ttcagaagaa gacgaatgct agtaaacaag aaatcaccct
     2821 tttaacgaaa gttatcggta tttctttgaa tagaatctct agtaactatc tgttgaagaa
     2881 gcccatcaat cgttacagtg ggtgttttga atatatccaa gaattcaatg aactgatcaa
     2941 tcagttggaa gaaatctatg aactaacttt gagccaatat gaaaaagatt ttatcagttt
     3001 tcctttgaac atcatcaaca caagtataac gatgataagg aaagctaact ctgatttgcg
     3061 cttatatttc gaccccatga tgatgcgtat tcgacgatta gatctcattg atctggatga
     3121 gtccttctta tatcaagaga tgaaacagca cctctcttta ttgataaatc gggtaatttt
     3181 tcgttatcga ttgactgatt tattttatgg tgagatagaa aaaaagtatc ctttttctta
     3241 ccaaatcgct agcgaatgca tacaagcatt agaagctatc ttgcaacata agatctctaa
     3301 agtagagatc agctacctaa cgttgtattt ggagttagca tttcgatatc aaaaagagca
     3361 tttttcctct aaaaaagtag ccattgttca tacgacagga aaaggaacag cattaatgat
     3421 ccaaagacaa atcaaaagga tattggggag ggatatacaa ataacacatg tttcagaaga
     3481 acaatatgaa aaagtagatc taaacaaata tttcgctgtt tttacaacga ttccgttaaa
     3541 aaatgtaaat gaagagacac cggtgatcca tttgactaat ctttttgatg actcttggtt
     3601 aagggcacaa tggcaaaaag caaaaaaaaa gcgattagcc gggtttcatt ctactgtttt
     3661 tttctatcaa atatggaaag aaaaaaagac gtatcaagaa tatttaaaca aattagtcgc
     3721 tcctttgatc agaggctatt atgtggatga agaatttgaa aaaaggatct tgaaaagaga
     3781 atatcagcaa agcacatttg ttaacaaaga atttgctttt cctcacacaa ccaaccagct
     3841 atctcaacac attgttttat cagtagggct aataactgat caaggtttag cagaaacagg
     3901 aggaaatgga gttatttttt tatttgccat acctgaagaa atgacggctg aagttgagaa
     3961 tgaattattt gagctatacg atatcctatt tgaactcata aaaatgggaa tagatgagtt
     4021 tattgaatta gcaccgacac aagaatcatt tttaaaacta atcaaaagta aaggagagag
     4081 acttcatgaa tgatatgtat ctattcatag ttttcgcagc ttcagcttat gtgctgcaag
     4141 cagttatcag ttggaagcaa ctaaagcatt tcaatcaaac atatagcgaa ctgcgaagaa
     4201 aaggaaaagt agcgattggt aaacgacctg ggaaaatccg ttcaggcacg attgttatgc
     4261 tagctataga cgatcatgat caagttttag aagtcagaaa aatgcaaggg gttacatctt
     4321 tggcaaaatt tcattcatta cctgtctatg cgggtgaaga tatccattac atcgatcgtt
     4381 accatccctc agttcgagcg gaagatcgtt tgacacaagc agctttagaa aatgccagag
     4441 aagtatactt acgaatagag gcaggaaatg aaaaaagtga acaaccttca ttgcctttta
     4501 ttgctatgct caataatttt cggatatgga aaagtacgat caaagaaaaa aggagtgaca
     4561 aacgatggat atcataacaa aatttgctga gggatttatg aggctgttcc aaacaggtgc
     4621 agaaacattc ataagctgga tgacaggaat cgtaccagtt gtcttgatgc tgatggttgc
     4681 aatgaatacg ctgattgctt ttttaggtga agaccgagta acgaaagtag ccaaattatc
     4741 agcgaaaaat ccgcttactc gttaccttgt gttaccgttc ttatctgctt ttatgttggg
     4801 aaatcctatg tcatttacaa tggctcgttt ccttccagaa tactacaaac caagctatta
     4861 tgctgctcaa gctcaatttt gtcatacgag caatggtgta ttcccccata ttaatcctgg
     4921 agaattgttt gtatggatgg ggattgctca agggatcgaa accttagggc taaattcgat
     4981 ggagctggct atccgatact tgcttgtggg actgctgatg aactttattg gtggttggat
     5041 aactgatttt actactggat ttgtatgcag acagcaagga atcgttctta gcaaaaaagt
     5101 agagctttca gttgattaga gaaaggagag ttttaaatgt atggtgatat taagatcgta
     5161 agaggaaacg gcgggtatgg aggtcctttg attattaggc cgacaaaaga taaacacaag
     5221 ttcatctatg tcacaggtgg aggtgagagg ccaatgatcg tagataaaat agaagaactg
     5281 acaggtatgg agtctatcaa tgggttcaaa acatcaatac ctgatagtga aatcgcgctt
     5341 gcaatcgttg actgtggagg gactcttaga tgtggaattt atcctaaaaa agggatacca
     5401 accatcaata tcgtttccac aggaaaaagt ggcccattag cacaatatat cactgaagat
     5461 atttatgttt cagcagtagg gttagaacaa atacagccac taatggaaac tgatgataac
     5521 aaagaaacag taaatactgc atctccagat aaaaagtaca aatatgatac ttctaaaaag
     5581 ataaccgagc aaaaagcaga gcagcaaaac tggatagcaa aaattggaat gggcgcggga
     5641 aaagtagttg ctacattcaa tcaatcagca agagaagcag tagatacgat gatccggaca
     5701 gtgatccctt ttatggcttt cgtttccctt ttgattggtg tgatacaagg ttctgggatc
     5761 ggtaatgtat ttgctaacat tatggcacct ttagcaggaa atgcagttgg attaacaatc
     5821 attggcttta tttgttcact gccattttta tctcctttgc ttggtccagg ggcagttatt
     5881 agccaagtga ttggaacatt gatcggtgta gagattggga aaggaaatat tcctccccaa
     5941 ttagcattac cggcactatt tgctatcaat actcaaaatg catgtgattt tattccagtc
     6001 ggtctaggtt tggaagaagc aaaagctgag acagtagaag taggtgtacc atcagttctt
     6061 tattcaagat ttcttaacgg catcccacga gttatcattg cttgggtagc aagttttgga
     6121 ttatatagct g
//
LOCUS       DAJSMI010000151         6033 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJSMI010000151 DAJSMI010000000
VERSION     DAJSMI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.153"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(129..776)
                     /locus_tag="ON430_002327"
     CDS             complement(129..776)
                     /locus_tag="ON430_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HCR3220226.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1184..1687)
                     /locus_tag="ON430_002328"
     CDS             complement(1184..1687)
                     /locus_tag="ON430_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3220227.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1810..2574)
                     /locus_tag="ON430_002329"
     CDS             complement(1810..2574)
                     /locus_tag="ON430_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCR3220228.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2682..2957
                     /locus_tag="ON430_002330"
     CDS             2682..2957
                     /locus_tag="ON430_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR3220229.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            complement(3158..3505)
                     /locus_tag="ON430_002331"
     CDS             complement(3158..3505)
                     /locus_tag="ON430_002331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220230.1"
                     /translation="MIPSVSFLFKKLKTFFILPEIEESNELTASDRSAFLLPVFNTWG
                     KAPQKQPYRRCSKVSFLVLRQIYAKMWKAIFTKFSLILKTKRLFHNQSLMLDADQLFH
                     NLIDGVLSAASRY"
     gene            3673..4602
                     /gene="yidC"
                     /locus_tag="ON430_002332"
     CDS             3673..4602
                     /gene="yidC"
                     /locus_tag="ON430_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR3220231.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            4705..6033
                     /locus_tag="ON430_002333"
                     /pseudo
     CDS             4705..6033
                     /locus_tag="ON430_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         1873 a         1154 c         1110 g         1896 t
ORIGIN      
        1 aagaaagggg ctgtccccag actttagtag gcgtcattag ctatctttcg ttagcattta
       61 aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt ctgtaaaaaa
      121 caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg agccaatttc
      181 gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt tttcgtatac
      241 gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg aaacattcga tgttttctga
      301 cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat cccttttaat
      361 ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt ccagccgatg
      421 tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt tttcactttt
      481 cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctgaac gatttgcttc
      541 agctcctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa aactgtcgtt
      601 aaaaatgtat cgatttctgg cagttcgtga tctaatatgt ctcgaatctt ttcagctgct
      661 agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc atttaataac
      721 tgttctactt ctcttattat aaaaaaatat tggtatggtt ttattgtttg ttccatgatt
      781 ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat cttctgcaaa
      841 gtatgtgtta cctgaagatc ctggactctt ttaaataaga tacgttttgc atcttatcat
      901 cacccccttt aatgcttatt attctatgta catgattcat tcgataaata aaagtaaacc
      961 gtatcttgaa cagatagtca actgcaaaca gaaaaaagga ctgtgacaaa agtcgtgtca
     1021 aagtcccttg tttcgaatga atggtgttca aaagcttgca tctgcagtaa taagccgaaa
     1081 cttcacaaaa atttgagaag taattttcgt aaattccttc ttatttcttg aagctgacca
     1141 cttctgtcac aacctcaata tggtctatta ttggttttac ctgttaaatc gaggattctt
     1201 tcccaaagta acgttgccat ctcttacgca tacttgcaga catgggctga gccgcttctt
     1261 tgactgcgca gtatttgtcc acgaatgtca caatatatcc ttctttatac ttaggcggtg
     1321 cgatcgttgc accccacatg tgcttcaaaa tgatatctcg ctcaagatcc gacaaatcgg
     1381 tgattttttc tgcattcttt acagcaattc ttggatggat atacgcatgt gtcccctcgt
     1441 caaatttcgt cgtacgccaa tcatagtaga acagatcatg caaaagtcca gcacgcgctg
     1501 ttgctttagc attgccaccc catttttttg cgagcagata actatagtaa gaaacactga
     1561 tggaatgctc caaacgagtg gaatgaacat gctgtgtgta attcgctaat tttttcactg
     1621 cttctgtttc taaaagatct tgtacataag acatatattc ttcgtcttca cgccattttt
     1681 ctgtcataat tatcgatctg ccaccttttt gaatttcatt ttcttctgaa attgatctaa
     1741 gtataccacc agtgttcgga ttattctagt cgctctagat aaaatagcta aagcttaata
     1801 gttccttaat cataaagaaa aatgataaag aacgatccct gctgcgactc cgacattcaa
     1861 tgattccgca tttcctttca ttgggatata tacgttctga tcagtcaaag acaagatttc
     1921 ctgactgact ccctgacctt cgtttcccat gattatagcg aaatcctctg ttttttctac
     1981 tttgtctaat gcatgtgctt cttcatttaa ttctgtaccg tacaccgtaa accctttgcc
     2041 tttcattaca gggattacat ctgccaaatc tttttgtaac acaggcaaat gataattgct
     2101 tccctgcatg cttcgcagca cctttgtact ataaatatct gccgttcctt tacctaataa
     2161 aacaccagcc ataccaaacg catctgccgt acggatcatc gttcccacat ttccaggatc
     2221 ctgtacatta tctagtaaaa gccagcctcc tgaaaaatct ggcgtatctt cagatgttaa
     2281 cgtcatcact gctaagatcc cttgcggtgt aggtagatct gacatagcgg tcatgacctc
     2341 atctgaaact agaaaagtag aaatctcttt gtttacagcc agccagtctc cccattcttt
     2401 catacctctc tgtgtcacaa aaagttcttt tatttctgca tcagacgacg cagcttcttc
     2461 gatcagatgg aacccttcca aaagatattc tgccttttct tctctgtatt tctttttatg
     2521 tagttttttt aattctttga ttcttggatt ttttgctgat tggatctctt tcacattcct
     2581 tcacctcacc tgattatatc atggaatagg tgcttgagct tgtttcatcc taaaaaaatc
     2641 ggtatcattg atttataaaa caggaaagaa aggtgacgaa catgcgcaaa gttaaaatga
     2701 atgtccaagg acgtgtccag ggcgtgggat ttcgctatat gaccaagatg gttgctgatc
     2761 agttaggcgt gaccggaacg gtaaaaaacg aagacgacgg ctcagtcaca atcgaagcaa
     2821 tcggaaatga cgacatcatc caaaaattta tcgaagaagt caagaagtct ccgagcccaa
     2881 gtggacgagt gcagtatgtg gatgtccaag aagatccgat gattgaggaa cgaaagaaat
     2941 ttgatgtggt gggataaggt tgtgaaaagg tcgcttagtt ttgagtatta aggaaaattt
     3001 tgtgaaaatg gctttccaca tttttgcata aatttgactt aataccgaga aactgacttt
     3061 tgaacaccgt cgataaggtt gtttttgaag cgctttgcct cgagtattaa ggagaaacat
     3121 tttcaaaata gcttctcata ttttgaaatg taatgactta ataccgagag gcagctgata
     3181 aaacaccgtc aataaggttg tgaaaaagct ggtctgcatc aagcattaag gactggttgt
     3241 gaaaaagtcg cttagttttg agtattaagg aaaattttgt gaaaatggct ttccacattt
     3301 ttgcataaat ttgtcttaat accaagaaac tgacttttga acaccgtcga taaggttgtt
     3361 tttgaggcgc tttgccccaa gtattaaaga caggtagtaa aaaagctgat ctatcacttg
     3421 cagtgagttc atttgactcc tctatttcgg gcagaataaa aaaagttttt agttttttaa
     3481 ataagaaaga aacacttgga atcactcttt tctttccaag tatttctttc ttgatttttt
     3541 gtcttttttc aaaatttttc gctacgaaaa agaaagaata tttatatgaa atttctatga
     3601 agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag
     3661 aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc
     3721 ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcagga
     3781 atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgagaat
     3841 ttcaattggt cttacggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc
     3901 ttgccactag gtatcagtca atcaaaaaaa acaatgatcc aatctgaaaa aatgcaagcg
     3961 ttaaaaccac aagttgaagc agcacaacaa aaactaaaag ctgcaacaac acgtgaagaa
     4021 caaatggcag cacaagcaga aatgcaacaa gtatatcgcg aaaacggatt gagtatgacc
     4081 ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtatttt
     4141 acggctcgct acacagaagg tatccgtgaa tcatcttttt acggcatcga tctaggctca
     4201 ccaagtatgg tgcttgttgc cattgcaggg gtcgcttatc tgcttcaagg atatatttca
     4261 acaatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct
     4321 gctatgatcg ttttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtg
     4381 ggtggtgtgt tcagttgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg
     4441 aaagcacaag tggcagaaga gttgaaacaa aatccgccaa aacaagtcgt cactccaaga
     4501 aaagacgtga cagaatcggc aaagccagct gacaataaac aaccaaaaaa cagtacaaac
     4561 aacaaaggcc gcaacgcagg aaagcagcaa aagcggaaat aaaaatatat gacaaaaaac
     4621 tgtagacaaa taaaaaaatt tctttgttct acagtttttt tgttacaatc aaagaaaacg
     4681 tcgcttttcg aggaggaatg tataatgatg tccaaataat caatggatag tcgaatggaa
     4741 gaagccaaaa tttcgctaga tgcacttgtt cctaaaaatc atttggtcca taaaattgac
     4801 aaaatattgt tatttgattt tatttatcca attgttgaat caacttactc gaccattggt
     4861 cgtcctagcg ttgatcctgt catcttagta aaattagtat tcattcaata tttatttagg
     4921 attcgttcga tgcgtcagtc cattaaagaa gctgaaacaa acctagctta tcgttggttt
     4981 cttgggctta gtttaatcga taaaattcct catttttcaa cgtttggtaa aaactatgta
     5041 cggcatttca gagaaaccga ttgtttgaac agatatttat cattatcctt gaacaagcag
     5101 ttgaagctag atttattcat gaagaaattt tgtatatgga ttcaacctat attaaagcga
     5161 atgcgaaaaa acatattttt acaagagaaa tggtacataa ataagccatt ttataccaaa
     5221 aacaattgaa agaaaaaatt aatgaagcaa gaattgcaga aggcaaacgt ccttttggtt
     5281 caatcattca tcaggaggtg gtagaaggga aaattagtgt cgcagatcca gaaagtggtt
     5341 attatgtaaa aacagaacaa gaaaaacagt ttgcttattc tgctcatacc gtttgtgatg
     5401 aaaatggttt tgtattagat gtcatgatta cgcctggaaa tctacatgac agccgaatgc
     5461 tcgtgccaca aatcgaacgt gttaaatcat gttgtggtgt agctgctgat gctgcgtaca
     5521 aaacaccttg gaacgccaag tatttaatcg atcgaaaact acgacccatt tttccttata
     5581 ctcgacccaa aagaagcaaa gaacgtttta aaaagaaaga tttttattat gatccctatt
     5641 ataactggta cctatgccca aatgatcaaa cacttcagtt tcgcacaact actcgagatg
     5701 gttataaaaa atatgtatct aaatctttta tttgtgaaag ttgtctatta ttgaaaaatt
     5761 atacagaaag ccagaaacac cagaaagaaa tacatcgaca tatttaggaa gactacttag
     5821 atgaagcaga acatttacgc catacggtgt acaatcgcga aaaatataaa aaaaagaaaa
     5881 gaaacgattg aacgcgtttt tacagacgca aaggaaaagc atggaatgcg ctacaccaaa
     5941 tatcgaggct tggaaaaaac caggtgcata cgatgcttgt ttttgccgca atgaatctgc
     6001 aaaaattagc catttggcag tgggggcata tgt
//
LOCUS       DAJSMI010000152         6007 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJSMI010000152 DAJSMI010000000
VERSION     DAJSMI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.154"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..1610)
                     /locus_tag="ON430_002334"
     CDS             complement(<1..1610)
                     /locus_tag="ON430_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR3220232.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQD"
     gene            complement(1773..2366)
                     /locus_tag="ON430_002335"
     CDS             complement(1773..2366)
                     /locus_tag="ON430_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220233.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(2367..2699)
                     /locus_tag="ON430_002336"
     CDS             complement(2367..2699)
                     /locus_tag="ON430_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220234.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(2749..5280)
                     /locus_tag="ON430_002337"
     CDS             complement(2749..5280)
                     /locus_tag="ON430_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220235.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            complement(5277..>6007)
                     /locus_tag="ON430_002338"
     CDS             complement(5277..>6007)
                     /locus_tag="ON430_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HCR3220236.1"
                     /translation="IQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKV
                     GNKTVEQENSTINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGH
                     KIRPYPIFNHGETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPD
                     IYARRLYHNMHDQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKL
                     RQNEEITEVIRKRLLLEAEKAVKE"
BASE COUNT         1626 a         1237 c          971 g         2173 t
ORIGIN      
        1 aagtcttgtt cttccatttg tttttcttta attaaacgtc gttgtcttct tccttgaatc
       61 aatgcctgat tccaaatcaa cgtcatagcc atgtacattt gacattgttg tacccctcga
      121 tctaagcctg taatagagtc aatgtcaaaa aagaccaact gttgttggtc aaggtgtcga
      181 atcgtactat gcccatcaaa aatcatgcca tacgtctcac ataaggattc tacgatggat
      241 tccatttctt cttttcgatt ctttttctct tgggaagcac ctggtaattc cagttgtcct
      301 aaaaagtcta aaaattcttt gaacgtcgga taagcttctg gatctttacc tgtgacttgc
      361 gtctccgtat ttgaacgcga atttgaggaa gtaaagccgc gtggtactgt tccatactct
      421 tcataaaaac gatttaagta ggtcttaaat tctgctttca tcgttccttt tagctgagga
      481 ctcactaact gtacctgata gaccactttg tctaagtgtg tttgatagga cgctgcttca
      541 ttgatcttca aatctttgac tgatccatcg tcatataaac tggatgcttc atcatacgtc
      601 cgtgtggcaa atatctctaa aggattgatc attccgtcag atccgtctaa acgcaccata
      661 ataccgttat attcctttaa gaaaggaata aagtcttttc ccttttcaaa gccccaaata
      721 aagtctcctg cagctaaatg ggcttccgcg atcattttaa gaacggtcga tttaccagct
      781 cccatcattc ctagaagcaa ggtattagta gacaaccgta cactattttt atacgtcgca
      841 ttccagacaa atgggccgcc tgtcatggtt tccccatatt gtgtccctcc tggatctttt
      901 agcgtttggt ggtggaacgg gaaacctccg ccaatgttta acgatggtag tgattgccct
      961 ttgatatttc cgaaccattc agcttgttca tcgaacatgg taaacatcga ttgccaatct
     1021 cgtttggttt tatgcagata agtcaccgca cgataatcca tcccttttag attttcgatc
     1081 agttcctgcg ctcgttcatc tacttcttga atcgtgtttc cgtaaacatg tactctcact
     1141 ttgacttgct tagaaatttc cccattagaa gtcaatttac gagcatattc cgctagttcc
     1201 aggtattctt ctgcgctttt atttcggtca gtcgcttttc gttcattatc tgatcgatca
     1261 tttaattcat ctaaggcacg attgatttga tcaatcactt tttgcttttc atctgtgcta
     1321 acatcgagtg tggccactgt atgaggattg ttcatgagat tggccaacca aaaaagcgta
     1381 ggattttttg tgtagtgata aactgtcaca catcgtgaat aaccattacc atacgtcaca
     1441 tattcttcat caaaatttaa cccaccttgt ggcatcactt gttttaaaaa atctaaatca
     1501 taccctttgg attttaaaat ttctatttcc cgatttccta gttgctttaa tggatcaagt
     1561 actttttgat cattttgtgg tgaaaaagct gttttaattg cttctaacat gtacagtcac
     1621 ctcttcttgt ttgattcttt acttggttaa aagattgccc ttttcccaac ttacccaggc
     1681 ttaaagtgtg aggaaatttt ctagtggtct cttccttcgg aacatctttt tctaaccata
     1741 tcaacggata tttcattctc tcttctccat tcctactcat cattcatatt gttcattcga
     1801 tacagaatct tgatcaattc ttttttgtag agtggtttta accctaaaat gccactagat
     1861 cgcattaact gacggtttct ggtttgtaaa tccttgattg tctttccttc aatgacgaaa
     1921 aagaagctta agtcaggacg ttcttgttct gctcgtttca attcctggat ttcttcgact
     1981 aatttctttt catatttttt taatcgttgt agatttcttc ctaaggactg ttccatacga
     2041 tttttatcta ataattgccg cttttgcacg atttgttccg ttagatccgt ctctgtggtc
     2101 aaactaacta acttaattgt tggcacatac gtacgcatga cggtggtcaa ctgattcatt
     2161 cgttctagtt tctcttgttc atttaatcct tccacgtctg tagaaactac acgataataa
     2221 cgttgaaaat gatcttctct agtaattaag actccctctt ctgtacagga tttatacgga
     2281 aataagtcca agatagagcg ctcttctggt tttccaaact gcaaaatgga tttcctttca
     2341 actgaatttt gtcctttgac tcgcatttat tcatcctcct tatacgtacc atgataattt
     2401 cggttaggat ctaacgcatg ataatggttc ttgtccgcaa aaaaaataga ctcgataatc
     2461 gcaaaaattt gtcgttgacc tgggttatta tagggtcgcc aaacaatcac taacgctatc
     2521 gcaatccata agccataatt caagagtgca aaaaagagtg gtaagtggac aagattattg
     2581 accattttcg cccaaaaaaa gcagccacca agcgttaata gatctaaaaa gaaaaaagcg
     2641 aaaaacttcc attctccact aaatcttgga gggtttttca tgtttatata tttatccata
     2701 attgatttct ccttttcata taaataaaag gcctaacttg taggcctttt atggtttgat
     2761 atcctgtttg atatccctca atagttgttc tttcaatgca ttgttcctgt ttttagtttc
     2821 tcttgtcaca tttatttttg ttggtgcagt aaatgccaca tttgctggct tgtaacctgt
     2881 atgaacgttt tgaataggtt ttggtgcagc ttctcttgtc acaacatttt gaacggacgc
     2941 attccctgtc tcaccacttt cagataaatt gtaagaaaca gttgtcagat ttccattttc
     3001 aggcaaacga cttggtttca tttgaacagt acctgcaggt gattgatgaa caaaactttg
     3061 atcattatcc ttgaattgat tcaaacgact atctttcatt ggtaaatttt gtgccttagg
     3121 taaaccaatt ttcgttgatt tttcagttcc aacttcgatt ccattatcat ttggcagaac
     3181 gatggaatcc tcatccgttt gtacttcttg atttcgtttt ggtaaatctg aaatgcgttt
     3241 ttcattttcg gagttaacag atctataccg atattgttct ggatcagttt ttggtaactg
     3301 tccctcattt tctgctttgt cttcttgatt tacttttgat aaatctgaaa ttcgcttctc
     3361 attttcaagg tcactgttag gtttatagcg atattgttct ggatcagttt ttggtaactg
     3421 tccatttttt ccttcttctg aatttctttc ttctatggaa tttttctctt caatcgaatt
     3481 ttcattagag tgcaacgctt cttggtgatc tttgactgaa ggaatcacta taccttcttg
     3541 attttctttt tcggttgttg tattagcttt tactgctgca ttaacttgtc cttcaccata
     3601 cgcatctttt agtccttctt tgacttctga agctttttct ttttgttttt ctagtgcttc
     3661 ggatggattc ttcacagctt cagccaattc tttagctctc gtgccgtcta atttctcttc
     3721 taaggtgtct ttggcttttt ctgtaagatc ttcagctgtt ccttttaacc cacgttcatt
     3781 gacgtaacca agaccttctg cggctttaga aacaccattt ttcgctttcc cgaacatttc
     3841 ggaaactgtt ttcttactat taggtacagt tgtagcttta tcgtctgatt gttcttcccc
     3901 gttatcaggg gtaacatctt ttcgatggcc aaatccattt ttacgaatat catttaaatg
     3961 gcttaaacca ctcttaccag cacttgcaag gtttttagct ccgctaccca catttttcgc
     4021 aatatttcct aaggcaaaca tgctaagaag agaattactg ccgtttttca gactcgtatc
     4081 aacaccgaaa atcttcgtaa ccgattgaga gcctttgaat agcgcaatag ttagcacaat
     4141 taaagcaaca acatacaaga atccattgat ctttcctgcc gcatgcaaat caccgatacc
     4201 tgaaagcatt aatgtataga ttcgtaattc taaaatcgta aacgccaata acaagaagga
     4261 ttgagaaata tcttctatcg cacttttcat ccgttggcct gtctcaaagt ccgttgacgc
     4321 ggcaatcact aaatagaatt ttttaaatac cagctcaata atgcaagtaa cgatcgtaaa
     4381 taggataaat aaataggcca ccgccaaaga agattcacca gctaagataa tacctggttt
     4441 actactgtat cgacgatacc ctgaagtata gacatatttt gctaagcctt cgtcactaat
     4501 ttgaacagga atttctttcc cattatcatc tagttccaat cggtattgta atgcctttaa
     4561 ccgatccttt tgtatatctg ataacccact tttatcatta cctgccatac ctttagcttg
     4621 ttcaggagta ataatcccat tgatattcgc tgttttaaaa tttttcgctg ttaaagcatt
     4681 tcttttatct gttggtaggg attctacttt attcgggtca gttgctagta acgtactgac
     4741 atcgatcaca ttattttgaa taatttgaaa agcaggtgat cccgttttat ctgctttatt
     4801 tgctgcagtg acgtctccgt aaaagctttt actgatattt aacccttcat cgattaaaga
     4861 actaccacca aaaatcaaga tcatggccat aaataaatta accaagatat ttttgacttt
     4921 tggtgggtgt ttactcataa aaaatttcaa gcctaaataa attaatgtga cgacacaaaa
     4981 aagagccaat accttactaa tcaaactttg aaatagtgca tttaccccag cggcgttcaa
     5041 taagtctttt aaatcaaagc tttgataaat ggttttttct agtaaagtat tgagattata
     5101 taatccttta atcaatcccc acagaaaagt gttccaaatc caactaaata catcttgata
     5161 ctgcagataa ttaccataat cttgtaagac tttcgttacg gttttggggt ttgcatcccc
     5221 atccccaaat aagcctgacc accagtcggc aatgccggaa aatattcctc caatgatcat
     5281 tctttcaccg ccttttcagc ttctagcaaa agtcgtttcc ttataacctc tgtaatttct
     5341 tcattttgac gcaatttttc aaggtattct tgcaaacgat ctatttgctt ctcttcataa
     5401 aattgaataa gtaacttttg tgccgttgag ttgtctaaag attgaacgat tttaataaaa
     5461 atgttttctt tagcttcctc ttctatttga tcgtgcatat tgtgataaag tcgacgtgca
     5521 tagatatcag gtagaaaata atcgtaatca atttgtaaag ccttaagatc caagtccgca
     5581 tgtgtacaat gtatgtctat atcattgata tcccacgatg gattgaaatc ttcaatatat
     5641 tgataagcat aaggaagatt cgtttcccca tgattaaaaa taggatacgg ccgtatttta
     5701 tgacctttca aatcttgtcg ttttaaagga gcgagtacaa tcgtttcacc ttctaaaaaa
     5761 gtagaaattc tttcaggagt taagatgcga tctgaatcaa cattgcgatt gatatgagta
     5821 ttcataccta atgaattgat ggtactgttc tcttgttcaa ccgtcttatt ccccactttt
     5881 ttggaaaatt cttctatggt attatcgtct gtcgacataa tataaatatg tgtctgacaa
     5941 ttttctttga taatcgctgc gttttgttcc ccatacttcg cataaagttg ctgataggat
     6001 tggataa
//
LOCUS       DAJSMI010000153         5944 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJSMI010000153 DAJSMI010000000
VERSION     DAJSMI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.155"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..842
                     /locus_tag="ON430_002339"
     CDS             <1..842
                     /locus_tag="ON430_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3220237.1"
                     /translation="KLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNIDL
                     FQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAVYL
                     LPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLMED
                     QLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGIGR
                     SILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSEEN
                     LSW"
     gene            1033..2220
                     /locus_tag="ON430_002340"
     CDS             1033..2220
                     /locus_tag="ON430_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3220238.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2226..2582
                     /locus_tag="ON430_002341"
     CDS             2226..2582
                     /locus_tag="ON430_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCR3220239.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2584..2922
                     /locus_tag="ON430_002342"
     CDS             2584..2922
                     /locus_tag="ON430_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCR3220240.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    2998..3254
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3317..4351
                     /locus_tag="ON430_002343"
     CDS             3317..4351
                     /locus_tag="ON430_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3220241.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4344..5030
                     /locus_tag="ON430_002344"
     CDS             4344..5030
                     /locus_tag="ON430_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3220242.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5054..5902
                     /locus_tag="ON430_002345"
     CDS             5054..5902
                     /locus_tag="ON430_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR3220243.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         1917 a         1085 c         1338 g         1604 t
ORIGIN      
        1 ttaagttatt aacgactttt cgagcagtat atgaaacaag aaatttttcc agagcagcag
       61 aagtactgtt tttatctcaa ccagcagtct cgaatcaaat caaacagttg gagcgggaat
      121 tgaatatcga tctttttcaa cgtaacggaa gaaaagagat gcttccaact aagcaagccg
      181 acttattata tgaacggaca ctggttttgt tagaagaatg ggaagaaact cagcaaaaat
      241 tgctcaatga acaaaatgaa gaagaagtat gtcggatcgt tgcttcccac acctttgctg
      301 tctatctttt gcctcagttg atgaaacatc tgatcaaaag gtttcctaaa gtgcattttg
      361 agatcgaaat cacgaattct catgaatcat tagaacaagt agcgaaacat gaatccgatt
      421 ttggttttat cgaaaagcca ttagagactt ccggcgtaca aagatactct ttgatggaag
      481 accagctagt catcgctggg gacccaaaaa gcaaattgtg gcttgtaaga gaagcaaact
      541 ctggtgtttt tcattacacg cagcgttatt ttgaagagat gaatatccaa gataagaaga
      601 tgatcgtgaa aagtaacgat gtaattgttg cgttgctgaa ggaaggaatc ggtcgctcga
      661 ttttgtctaa gcgctctgtt ccagaaaatg tgccaatgac tccgttgaat ttccatcgat
      721 atttttattt catccaacga accaatctgg tttctgatca gctgctagag attgccgaag
      781 agatccttcg atacaatgaa agcacagaaa cttctcacag cgaggagaat ctcagctggt
      841 agataattgc atgtatcaaa aaaagttcta tgatacacta aagataagca gtaggctttt
      901 gtactgcatt tgctcaagca actgcacaaa cggaaagtta atttttccca aatgtctatg
      961 gaaagacttt ccattatcta actggaccgc tataataatc agcagtatgg tttttaagaa
     1021 aagggatgaa acatggaaaa aaaattaaaa gcttcgaacg ataatcgtat tgattatggc
     1081 gtgattttac cagtctttct gttatgtttg attggtctgc tctcattata tgtagcactt
     1141 agccatgatc cgaacaatcc agcagtcttg aaaggcgtag ggatgcaagt tgtctggtat
     1201 ttagttggag cggcagcaat cgttgtcatc atgcatttga attccaaatg gatctggaaa
     1261 ctgactcctt acctttatgg tttggggtta gtggtaatgg gactcttgct caaattctat
     1321 gatcagaatt tagctgcttc aacagggtct aaaaactggt ttcgattcgg tgcgtttact
     1381 ttccagccag cagagttgat gaagattgcg tacatcttga tgatggcact gatcgtaact
     1441 aaacataata cgcaagtaaa agaacggacg atgaaatcgg acttttggct gattgggaag
     1501 ctcttgatcg tgacaatacc ggttttagca ttgatcatgg cccaagatga ctttggaacg
     1561 atgcttgtct ttcttgcgat ctttggcggg attttcctga tgtctggaat ctcttggcgc
     1621 atcatcgcac ctgtagtaat cttggcagta gtcgttggag caggaacgat cttcttggtt
     1681 acgactgaag gcggaagaga cttgctttat aaagttggat tcaaatctta tcagtttgca
     1741 cgtattgatt catggctgga ccctttccat gatacgtcag gaatgagtta tcagccggcc
     1801 caaggacttt tggcaattgg taccggtggc ttgtttggta aaggattcaa cgtaagcaat
     1861 atctatgtgc ctgttcgtga gtccgatatg atttttaccg ttatcggtga aaacttcggc
     1921 tttatcggtg gtgcattcgt cattttcttg tattttattt taatctatcg tatgattcgg
     1981 gtgtgtttcg atacgaataa tgaattttac gcctatatcg caagcggttt gatcatgatg
     2041 ctgctgttcc atgtctttga gaatattgga gcaaatatcg gattacttcc tttaacaggg
     2101 atcccattgc ctttcattag tcagggagga tcctcgattt tagggaacat gatcggtatc
     2161 gggttgatat tatcgatgag ataccaaaat gaagcacctg caagatcttc aaggaggtaa
     2221 gaaaattgta caccttttac tggtacccga aatgttcaac atgcaaaaaa gccaaagcgt
     2281 ggttagacca gcatggcgtt agttatcaaa ccgttgatat gatcaaagaa gtaccgaagg
     2341 cctcaacgat tgagacttgg ctagaaacga gtcaagtacc aatccgtcgc ctgttcaaca
     2401 caagcgggat gaaatatcgg gaacttggac tgaaagatca agtaggcgag tttacggctt
     2461 caaaagcgag ccaagtctta tcgacagatg gtatgctgat caagcgacca ctgatcttac
     2521 gagaggacca gcttgttgca attggattca atgaaaaaat ttatgaagga gaactgatat
     2581 aaaatggcag gcaagcattt gaagaaaaag gacaaccttt ggatcttatt taatggaaaa
     2641 gaatactgtg ttggtttgac aaatgaagcg caagaagaac ttggagacat cacgtttgcc
     2701 aatctgccaa aagcaggaca atcgctaaaa aaagatgaac ctctgatcga agttgaagcg
     2761 gaaaaagcgg tcaatgaatt tgccagcccg ctaacgggta cagtttcttc tgttaatgat
     2821 aaaatcggtg aagatatcaa tatcttgaat gattcagatg aaatgaatgc gtggatctta
     2881 agtttcaaag atgttgatcc aaaagaattt gacgaattat aaaatcagaa aatcacttga
     2941 aaaaagttga cactgattct tccagttgct ataatcataa gcaatcctat atgaaacgag
     3001 aaagctttga aaagaagagt acatgtcacg gacgtttgaa gagagccctg tttgctgaaa
     3061 atgggtaacg aaaatgagat ggaagatggt cttagagcag agtaagcgag cgtaagcaaa
     3121 cttactacgg gagtgcccgt tacagcgcga cagtatacgg aaagaagcta agtccaagta
     3181 ctggaagagg ctgttattgc gaagtaacgg taaatcaagg tggtaacacg gaagttcaag
     3241 ctttcgtcct tttgcttaag gcaagagggc gaaggctttt ttgtttgaaa aattataaga
     3301 gaaagatgtg aatggcatgt cattaatcca actaacgaat gtcaaaaaga cattcaatgg
     3361 gaaaaacgga aacatccaag ccgtcaatga cgtttcacta aacgttgaaa aaggggatat
     3421 ttatggaatc gtcggctatt ctggcgcagg aaaaagtaca ttagtccgtc tcctgaatgg
     3481 acttgagctt cccacgagcg gcgaagtgat cgtaaacgat caagatatta caaaattaaa
     3541 aaacaaagaa ttacgtacat tccgaaagaa aatcggtatg atcttccagc atttcaacct
     3601 gttatggtca agaactgtac ttgaaaatat ccagctgccg ctggaattag ctggcgtacc
     3661 aaagagcaaa cgtaaagaac gggcagaaga actgttgcgt ttagtcgggt tagaaggacg
     3721 cggacaagca tatccttcac aactttccgg cggacagaaa caacgcgtcg gtatcgcacg
     3781 agcattggca aatgatcctg agatcctgct ttgtgatgag gctactagcg cattggaccc
     3841 gcaaacaaca gaagaagtat tagacttatt gttagcgatc aataagaaat tgaatttgac
     3901 catcgtcttg atcacacatg aaatgaatgt gatccgcaaa atctgcaaca aagtcgctgt
     3961 gatggagttg gggaaagtcg tagaagaagg agacgtattg actgtcttca gacaaccgaa
     4021 aaaagaagta accaaacgct ttgtacaaca agacatcgaa ccagaagctg attcgacaga
     4081 actattagct gaactcatca aagaaaatcc aagcggccgt ttggcaactt tgacctttag
     4141 cgaaagaaat gccaacgagc cagtcatttc ccaagcgatt cgacgctata acatcgatgt
     4201 caacgttgtg tatggaaaga tcaaacaagt gaaagaaggt tcctttggtt cattgactgt
     4261 cttgatgact ggtgaaacca aagaattaga tcaagcagaa gtgttcatca aagaacaagg
     4321 agtaggaatt gaggtgatcc atcgtggata aaagcttttc agaaacatat ctagatttta
     4381 aacaaatcaa cccggaaacg atgaatcagg ctatccttga tacactatat atgacattaa
     4441 tctcaatggt cttagtgacg atcatcggat tagttttggg attgatcctt tactctattg
     4501 gacgcaaaaa caaaccgtat gctcgaatca tttatggaat cgtatcgatc atcagtaata
     4561 ttttccggtc aacgccattt atgatcctga tggttttgat cattccattt actaaagcaa
     4621 tcgtaggcac tatgctaggt gcgcaagcag ccattccagc tcttgtttta tccgcagcac
     4681 cattttatgc ccgtctagta gagattgctt ttcgtgaagt cagccctggt gtactggaag
     4741 cagcagatgc aatgggagca agttactggg aaatcatctg gaaggtcttg atacctgaaa
     4801 gtattcccgc attgatttct ggattaacag ttacaacgat ctcgatgatc ggatttactg
     4861 cgatggccgg cgcgatcggc gcaggtggac ttggaggtct tgcttggcag gaaggctacc
     4921 agcgtggtaa tctaacagtg acattcgttg cgactttaat tattttacta atcgtattta
     4981 tcgttcaagg aatcggtgat ttcttaacta agaaaacaga caaaagataa taggatatag
     5041 ggggatgtag gatatgaaaa agaaaatttt tggatttgca gcagcgcttt tgctgactgt
     5101 aggattagca gcatgcggta acaacagcga cagcaaagat tcagcaaata aagcagacga
     5161 cacgacattg aaagtcggcg cttcaccgac accgcacgca gagattttgg aacatgtgaa
     5221 accattattg aaagatgaag gaatcgatct tgaaatcgtc aaattcgatg actatgtatt
     5281 accgaaccaa tctttggaag aaggcgacat tgatgccaat tacttccagc atattcctta
     5341 tttgaacaaa gaaatcaaag aaaaaggata tgactttgtc aatgccggtg ctgtccacat
     5401 cgaaccaatg ggcttgtact ctaaaaaagt caaagatgtt tccgaattaa aagatggtgc
     5461 aacaatcatt acttcaaatt ctgaatcaga ctggggacgc atcatcacaa ttctacaaga
     5521 tgctggctta gtcaaagtca aagatggagt agatctggaa acagcaacat tcgatgatat
     5581 tgaagaaaac cctaagaact tgaagttcaa ccatacgatc gacccatcac ttttagcttc
     5641 tacgtaccaa aatgatgaag gtgacctagt tgcaatcaac gcaaacttcg cttacggcgc
     5701 agggttgaat ccattagaag atgccgtatt gcttgaaaag gacaactctc cttacgtgaa
     5761 tattattgcg actcgtaaag gtgatgaaga cagtgacaaa atcaaaaaac tgattgaagt
     5821 gcttcaccgc aaagaagtcc aagaatggat cacagacaaa tggggcggct ctgttaaacc
     5881 agtaaaggct gatgcaaaat aaggtatgga acaaaaaaga tgagaaataa gtctgtaatg
     5941 gctt
//
LOCUS       DAJSMI010000154         5941 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJSMI010000154 DAJSMI010000000
VERSION     DAJSMI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.156"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            213..1487
                     /locus_tag="ON430_002346"
     CDS             213..1487
                     /locus_tag="ON430_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3220244.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1541..2968
                     /locus_tag="ON430_002347"
     CDS             1541..2968
                     /locus_tag="ON430_002347"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR3220245.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2981..3853
                     /locus_tag="ON430_002348"
     CDS             2981..3853
                     /locus_tag="ON430_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3220246.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3948..4943
                     /locus_tag="ON430_002349"
     CDS             3948..4943
                     /locus_tag="ON430_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3220247.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(5548..5655)
                     /locus_tag="ON430_002350"
                     /pseudo
     CDS             complement(5548..5655)
                     /locus_tag="ON430_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5771..>5941
                     /locus_tag="ON430_002351"
     CDS             5771..>5941
                     /locus_tag="ON430_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3220248.1"
                     /translation="MIIKNLGNVKSIDIHVKDLLIFTGKNNTGKTYTSYILYGILSEL
                     FGARFFNYFSHTE"
BASE COUNT         2070 a          806 c         1129 g         1936 t
ORIGIN      
        1 atttatttat aaaaaatagt gccattaaaa aagaaaagtg acacaccaat aaaagtcata
       61 tcctgtgaca ttatatggca ttatccaaat agaataaccg gttgacatgt aagcggattc
      121 cttttataat tttaggagta taagtccttt tctaaaaata ttaaccggtt atcgtgaaag
      181 gggatacaaa tatatcgttg gaggataaaa atatgtcaaa ggttaacgta tttaaaaatc
      241 aatcctattt acaaagttca gctacattat tactattttt tgcttcttgg ggtgtttggt
      301 ggtcattttt tcaactttgg ctaacatctg aatcaaatgg tttagggtta tctggcagtg
      361 ctgtaggaac agtattctcg gcaaattcgt tagttacctt aattttgatg tttatttatg
      421 gaacattaca agataaattg tatattaaac gaaatttatt aatttttgct tctgtattag
      481 cgacacttgt tggaccattt tttatatgga tatacgggcc attgctagat aacaatttta
      541 atttaggcat tattatggga gcactatttt tgtcagctgg atatttagct tctgtaggag
      601 tttttgaagc tgtgtcagaa aggtttagtc gtttatttgg ctttgaatat ggacaagcaa
      661 gggcgtgggg atcatttggt tatgccttgg tagcgctttt ggcaggattt ttatttgtaa
      721 aaaatcctca tttaaacttt tgggcgggat ctttctttgg ttctttacta ttgttaaatt
      781 tattattttg gaaccctaaa gttgaacggg aagcaaatca aaattttaat caagaacaag
      841 ctgaatcaaa tagtattcct tctttaaaag aaatgtttga tctaatgaaa ctgcctcaat
      901 tatggacgat aatcatcttt attgttttta catggacatt ttatacggta ttcgatcaac
      961 aaatgtttcc gggattttat actggtttgt tttcaacatc agctaatggt gaaaaaatat
     1021 atgggacatt gaatgctatt caagtatttt gtgaagcgtt aatgatggga attgttccaa
     1081 tcattatgag aaaattaggg gttcgaaata ctttgttatt aggtgtaacc attatgtgtg
     1141 tacgaattgg attgtgcggg tttgcctcga caccattatc tgtttcatgc ataaaaatgt
     1201 tgcatgcttt agaagtacca ttatttacat taccaatgtt tcgctatttt acacttcatt
     1261 ttgatacaaa gctatcagca accctctata tgataggatt tcagatagct gctcaaattg
     1321 ggcaagtgat tttatcaaca ccattgggaa tattaagaga caacgttggc tatcaaccaa
     1381 catttaaaat tatttctctt attgtattac tagcaggcat atatgcattc tttattctta
     1441 aacaagatga tagagatgtt caaggggatc catttattcg aggataatgg aaaccaacag
     1501 aataaaaaac aattttatct agaaagtgag cgaatatatt atgacaaaaa gagctataga
     1561 attaacaaat acacgttatc gtttgggata tcatgtgtct gcaccatcag gatggattaa
     1621 tgatccaaat ggattttgct attttgatgg gtattatcat gtgttttatc aacatcatcc
     1681 atatagtgct gaatggggac caatgcactg ggcacatgct agaagtaagg atttagttca
     1741 ttgggaatct ttaccacttg ctttgactcc tggagatcaa gaagatgaag gtggttgttt
     1801 ttcaggaagc gctattgaaa aaaatggtgt gttgtatctg ttctatacag gacatcacta
     1861 ttatggagac aatgatccag atcatttttg gcaaaatcaa aacatggcgt atagtacgga
     1921 tggcattcac tttactaaat atgaaaaaaa tcctgtaata gcaaaagcac cagaagataa
     1981 cacacatcat ttcagagatc caaaagtatg gaaacacgat gaaaaatatt acatggtttt
     2041 gggtagtcaa gaaaaagatg gattaggtcg tgctattgtt tatcagtcgt tagatttatt
     2101 aaattgggat tacattggac caatttctaa agcgaaggaa gtacttacag aaggatttat
     2161 gtgggaatgc ccagactttt ttgaattaaa tggaaaatct attcttttat tatcccctca
     2221 gggaattgaa gcaaatggaa aagattattt aaatctatat gaaacaggct actttatcgg
     2281 tgattatgat tatgagacag caaaattcat tagaggaaat ttttatgaat tagataaggg
     2341 acacgatttc tatgcaacac aaacaacttt atctccagat ggtagacgta ttgttatggc
     2401 ttggatggat atgtgggaaa gccctatgcc tgaaaaagta gacggatggg caggagcatt
     2461 aacgcttcct cgagaattag agttaattgg agatcatttg tatatgaaac ctgtgagaga
     2521 attagaacaa cttcgagttg gcagcggcgt tgaaacaaaa gttaatttgg caggagaaat
     2581 aataatcgct caagatgctt cattcaaaga agctctaatt gatgtactat tgactaaatc
     2641 aaaaaatcaa gaaatcaaat tttcattaaa aacaaaagat gaagagttaa ttaatatttt
     2701 ttattctaag gataaaaatg aatgtacctt aactcgtaac ggcaaaaatg atccaaggta
     2761 tggaaccatt cagatgtgtg atagattatc acttcgagtg tttatcgata aaagttcggc
     2821 agagattttt attaatgatg gcgagcttgt ttttacagag cgttattata cagaaaatca
     2881 accaagtatg catttgagtg ttagtgaaga agcttttgtc tcgagtacaa tttatcatct
     2941 aaaaaaagat gcaatttctt acgagtaagg aggttttatg atgttaattg gtagcataga
     3001 agcaggtggg actaaatttg tatgtgccgt gggagacgag gattaccgag tacaagacca
     3061 aataatttgt ccaacgacca cccccaggga aacattgaaa aaaactgttg actattttaa
     3121 acagtttgat ataaatgcat taggtattgc atcatttggt ccaatagaaa ttcgaaaaaa
     3181 ttcaccaaaa tcaggatata ttacgtctac accaaaggtg ggatggcgaa atgttaatgt
     3241 gttaggttat ttacaagatg agctgaatgt tccaatgtct tggacaacgg atgtgaacgg
     3301 atcagcttat ggagagtaca tcatgtccac tttatcgaat gaaaaaataa attcattagt
     3361 ttattacacc gttggtactg gcgttggtgg tggagctgtt gttgacggaa aactattagg
     3421 atcgtttggt cattcagaaa tgggccatac gtttgtaaaa cgccatcctg atgatattga
     3481 ttttaaagga atttgtcctt atcatggtga ttgtttagaa ggcttagttg cgggaccaac
     3541 gtttgaagca cgttttggaa aaaagggtga agacgtttct ttaacggatc ctatttggga
     3601 tgtaatggcc tattatctag cacaagctgt gattcaaaca acattgatta tacgaccgga
     3661 taaaatagta tttggtggaa gcgtagtaaa tgaaaaattg ttagaaaaaa ttcgtgttaa
     3721 tgttaatgaa ttattggatg attacgtgga aataccaccg ttaagtaaat atattacgcg
     3781 tcctttaatt aaaaataatg gatcggcaac actaggaaac tttgctttgg gcttgaatca
     3841 attacaagaa taaaacatag aaaaaatatc gattatcagg tatagtagtt ctatagagtt
     3901 ctttttttcg atagttaatt attgcattat ttaggagaga caagagaatg cgcccaaaat
     3961 tagaagatgt tgccaaaaga gcaaatgtat caaaaacaac tgtttcacgt gtattaaaca
     4021 atcgtggcta tttaagtcaa aaaacgattg ataatgttta taaggctata gaagaactca
     4081 attatcaacc gaacgttgta gcgcgtcaat tatttcaaaa gaaaactaac attgtcgggt
     4141 tactatttcc tacagtagcc aatccttttt ttagtgaact cgttgaggca ttggaaaaaa
     4201 agttatatga aattggttat aaggtattaa taggcaattc gatgaataat aaagaaaagg
     4261 aaacaaatta tttaaatcag ttattgtctg atcaagtaga tggtttaatt gttggtacac
     4321 ataaccaagg gattcaggaa tataaatatc agaatttacc gattgttgca attgatcgag
     4381 tgatgaatga agatattcca gttgttgaat ccgataacta caatggtgga aaacttgcaa
     4441 ccaagttatt aattgcacaa ggtgcaaaaa atattattca tacaaacggt ccaattgact
     4501 tacagactcc ggctaatcgt cggagactag cctatgaaga tactatgaaa gcatatcagt
     4561 taattccacg aactgtgaca ttagatttca atatttctta tgtaaagaaa aaacagattt
     4621 ttttccaaat gtttgaggat tatcctaaaa tagatggtat ttttgcttca aatgacatcg
     4681 atgcggccct aatattacaa gtagcaaaag aaaaggggtt aaatgtacca gctgatttat
     4741 tagtagtcgg atatgatggt actttaatga ctcggagtat attaccagat ctgacaacag
     4801 taatacaacc aattaatgat atagcagaca cagctgttgc tattttgatg aaacgaataa
     4861 ataaagaaga aacaaaaaag gaatatattt tacctgtcac attatggatg gggaagacat
     4921 cagtaaagaa aaaggacaat taaatttata aagactatca atgataattt cattattaat
     4981 tcgtgatata aaaagttgtt aaatctctta taatagacta tgatacaaaa tcatgctttt
     5041 aatctgtgta gatgttgcca aatatttgaa agacatagaa cttgtcagaa aaaccttaac
     5101 aattcctcaa tgggcggata agctaggcaa aagggctggc ataaactttt cagtccttct
     5161 aacagaatca attgcagata aagcagataa cattcttctt tctggaagaa ataattagaa
     5221 ctcaaaaggg tcagaaactt aaaagttttt gatcctttat tttcaagtct gatttgacag
     5281 attttcggca gctcatttat ttatggtcag aaatagttgc tgaaatttta atctattagc
     5341 aaatagtcca gaacgtatga agaggattcg gatatcattt gtcattaaca ccataaatat
     5401 ttataaaatt cgtgttcacc tacaattaaa tttaatatat aatattcgtt gtaagggctt
     5461 ataatttttt gttaatgaac aagatatgta attgatagtg tcaatagcta ttcaaaaatg
     5521 taggcttttg gcccataaat aacaaggtta cttatatgat aacttttttt ctttataatc
     5581 tttaatccga tagagctttc cagtagtttg aataatctta gcgtggtgca ctaatcgatt
     5641 gattagtgca tttgtgttca gtccattgag acataatagt ggatagtttt actgagaaaa
     5701 ttgataaaaa atagagatat aattcaaata cgtatataat gaaactaaag ataaatacgg
     5761 aggaaaatat atgattataa aaaacttagg aaatgttaaa tcaatagata tacacgttaa
     5821 agatttgctg atttttactg ggaaaaataa tacgggaaaa acatatacca gctatatttt
     5881 atatggaatt ttatcagaac tctttggagc aagatttttc aattattttt ctcatacaga
     5941 a
//
LOCUS       DAJSMI010000155         5912 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJSMI010000155 DAJSMI010000000
VERSION     DAJSMI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.157"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            55..126
                     /locus_tag="ON430_002352"
     tRNA            55..126
                     /locus_tag="ON430_002352"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:87..89,aa:Gln,seq:ttg)
     gene            172..242
                     /locus_tag="ON430_002353"
     tRNA            172..242
                     /locus_tag="ON430_002353"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:204..206,aa:Cys,seq:gca)
     gene            263..347
                     /locus_tag="ON430_002354"
     tRNA            263..347
                     /locus_tag="ON430_002354"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:297..299,aa:Leu,seq:caa)
     gene            409..481
                     /locus_tag="ON430_002355"
     tRNA            409..481
                     /locus_tag="ON430_002355"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:442..444,aa:His,seq:gtg)
     gene            491..562
                     /locus_tag="ON430_002356"
     tRNA            491..562
                     /locus_tag="ON430_002356"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:523..525,aa:Gln,seq:ttg)
     gene            580..664
                     /locus_tag="ON430_002357"
     tRNA            580..664
                     /locus_tag="ON430_002357"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:614..616,aa:Leu,seq:caa)
     gene            919..1794
                     /locus_tag="ON430_002358"
     CDS             919..1794
                     /locus_tag="ON430_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3220249.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1898..2662
                     /locus_tag="ON430_002359"
     CDS             1898..2662
                     /locus_tag="ON430_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCR3220250.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            2649..4091
                     /locus_tag="ON430_002360"
     CDS             2649..4091
                     /locus_tag="ON430_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3220251.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            4102..5433
                     /locus_tag="ON430_002361"
     CDS             4102..5433
                     /locus_tag="ON430_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCR3220252.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            5446..5904
                     /locus_tag="ON430_002362"
     CDS             5446..5904
                     /locus_tag="ON430_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3220253.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1924 a          925 c         1270 g         1793 t
ORIGIN      
        1 attgtggctc cggcattcgt gggttcgatt cccatcattc gcccttttta ttcttggggt
       61 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
      121 ccccagtttc tataatttat tctttatttt atagattgat aatagtataa tggcggtata
      181 gccaagtggt aaggcagagg tctgcaaaac cttcatcacc ggttcaaatc cggttaccgc
      241 cttagtacta ataaatcaac ttgccggcgt ggcggaattg gcagacgcgc tggactcaaa
      301 atccagtgcc cgttgagggc gtgccggttc gaccccggcc gccggtattg actaagatgt
      361 cttcatctta gttttttatt gagaaattaa ataacatctt ttatgatggc gaatgtggcg
      421 aagtggttaa cgcaccggat tgtggctccg gcattcgtgg gttcgattcc catcattcgc
      481 cctttattct tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt
      541 ggttcgaatc cagctacccc agttattatt tttaaatttg ccggcgtggc ggaattggca
      601 gacgcgctgg actcaaaatc cagtgtccgt tgaggacgtg ccggttcgac cccggccgcc
      661 ggtataatta tcaatatagc aactttctta tagagagttg ctatattttt tttgttttta
      721 tgtaattacc tccaaatcaa ttcgatacaa gtattatcat aaaacgagta aaaaagatat
      781 tgataacgct tttattatgt gttaaactat ttttgtaaat tatgtagggt gctactattc
      841 aattaggcac gacctaagaa agggttcttt cttaggtcgt tttttgtctt taaaaattgt
      901 ttggagacaa aaatatgtat gaaggagtct gcaagttgtt ttgttttatc atatagagga
      961 ggtgaattaa cggtgtatgt gatgaaaaag ctgaatcata atgctgtatt gatcgatgac
     1021 gatggaatag aaaaaattgc gatggggaaa gggattggct tttctgctaa agtgagtaag
     1081 ccatttgatg ttgaggatgc tgacaaatta tttgtattag attctaaaga aaagatcaaa
     1141 cgattcagtg agatagtctc gcagattcca atcgaattta tggattttgc ggaagaagtg
     1201 attgaactga taacagctag gatctcctct ccattagatt caaatatcta tattacattg
     1261 acagatcata tttattttgc tattcaacga aaaagagaag atagagatgt cacagcaatt
     1321 atgttgccag aaatgagatt cctttatcca aatgaatatg atatagcagt agaagtggtc
     1381 gagcgaatca atcatcgata tcaaaccgag ttagggtcaa atgaagtagg attcatcact
     1441 atgcatatca ttaatgctga gctaggagag cctaatagtt taaagagctt aaaaatagta
     1501 gaaatgacga ccacgatttt gtcccatcta gaaaaatcct attcaaacgt ttatgataaa
     1561 gaatcactga cttatcatcg cttaatggtt catatcaaat tcctggttaa acgcttgatt
     1621 tatcaggaag aaacgccagt agaaaatatt ggttttttta ctgaaacatt taaacatagc
     1681 aaagcgtatt gtacagcact aaagattaaa gatatcatcg aacagcaatt tcaacttgcc
     1741 attcaagaaa atgaaatcat ctatttggca attcatctag caagaatcta ttaaaataag
     1801 ggcgtaacta tctataggcg caaccaaatg acacatagta aatggactat gattatttgg
     1861 ttgcgcctat tttagtagaa aaaagaagga gaatgaaatg ggaaaagtga tttttaattc
     1921 ggatgatttt ggctatagcc atggtgtgaa ttatgggatt atggatgctt atcagcgagg
     1981 aattttgaca tcaactacat tgatggcaaa tatgccgggt ttcgagcatg ctgtgaaact
     2041 aagaaaggaa atgcctaggc taggtgtagg agtccattta acattgacgt gtggcaaacc
     2101 actcctcaaa actgtcgata ctcttatcga aagcggagag ttcagatcct tatcttatta
     2161 tcagcatcca tttaacattg acaaagatca actgtatcag gaatggaatg ctcagattca
     2221 aaaaatttat catgcgggga tcataccaac tcatttagac agtcatcatc atactcatac
     2281 tttggatgaa aatcaggagg tagttgttac attagcaaaa aaatatgatt tgccggttcg
     2341 gagcaacttt gaacgaaaag atgaagttcg tcatgtcgat tattttgagc cttgtttcga
     2401 tgtcgtagga gaaactgatg aaaaaaaacg acaaatcaat acgagtgtgg aagaatattt
     2461 ggcaagttta ctcatgatat tgagagaaga taaaacgatc gaagtcatgt gtcacgcagc
     2521 gtatttggat caggaattgt taaatggttc aagttttgtg tatcctcgta cccaccaagt
     2581 cgaattttta attcattcag catttgctca aaaagtgaaa aatgatcctt tgattgagtt
     2641 agtaacatat gaggatattt aatagaggag gaataaaaat gaatgttaaa tcaaatgtgc
     2701 agaagcttgg aagagcgatg ctacttccag ttgcagcgat gccattagcg ggattgatca
     2761 tgcgtttatc agctgacgat atgctgaaca tcccagtaat tggtgcagct ggaaatgccg
     2821 tttttggaaa cttggatatc ttatttgcta taggagttgc aatcggtttt gtcaaaaata
     2881 aggacaaagg aatacctgca cttactggtt ttttagcgat tgctaccttg aaaaaaggct
     2941 tagaaattat ggacccagaa gtgaatatgg ggatttttgg tggaattatt tccggtttag
     3001 tagcggcatg gacgtataat cgctttaaag aacaaaaatt accagtagtt ttttcttatt
     3061 ttgcgggcga aaaattccca ttgactatgg tgatgattct acaaacaatc gcagctgttt
     3121 ttttgggctt tatctggccg ctcatccaaa ttgggattga taattttgca aaactgctcg
     3181 ttgatatggg agcattaggt gtggggatat ttatgttttt gaatcgtttg ctgatcccct
     3241 ttggactgca ccatgtgttg aatacgtata tttattacga tttaggaagc tatacatctc
     3301 ctagcaagga agttttcaga ggagagatga cacgctttat caacggagat cctcaggctg
     3361 gactgttttt atcaggtttt tttgtagtaa tgatgtttgg tataccagct atatgtctag
     3421 ctatctataa agcagcttat aaagaaaata aagagatggt caaaggaatc atgggaagcg
     3481 gtgcaggaac ttcatttatt gcaaatatca ctgagcctgt tgaatttagt ttcatgtttc
     3541 tttctcctat gttgtacgtg atccatgcac tttttgccgg tttagcaggg attgtctgtt
     3601 acttattcaa tatacgaatt ggttttacat tcggagcatg tattgttgat tatctcatca
     3661 attttcgaat cgctaccaat gctattttga ttcttccaat cggaattttc ttttttgcct
     3721 tgtattacgt tactttttat tatttgatca ataagagaaa cattcagaca ttaggacgtg
     3781 aagctaaggc agagtttggc aatgaagtaa ccttagaaga aacagagtta gggttagcaa
     3841 gtaaaaatta tgaatatatg gcaacaaaaa tgctacaagc gtttggagga aaagccaata
     3901 tcctggatgt ctatagttgt aatacacgcc tacgggtaga ggtagtggat cccaccatgg
     3961 tcgaggaaca gcgaatcaag caattaggga taagcgggat cattaaacca actgaaaaaa
     4021 attatcaaat tatcatcggc ttagaagtga cttatgttat ggcagaattc aataaattat
     4081 tagaagaata gaaaggaaaa gatgatgatg aaaaaacaga ttatcactat agctggaggc
     4141 gggagtacgt atacaccagg aatcgttcaa gccattttga acaatgaaaa acggttgcca
     4201 ttatctgaaa tacgtttata tgatattgat gaagaaagaa atatggatat gtacttgatt
     4261 gttaaattta tgctgaaaag aaaaggtttt tcaaatattc gcattcgtgc aacggatgat
     4321 ccaaagcttg cttttacagg atgcgatttt gttttctcac aaattcgtgt tggaggattg
     4381 gagatgcgag aaaaggacga aaagattcca cttaaacatg gattagtagg acaagaaacg
     4441 tgtggactcg gaggatttgc ttatgggatg cgctcaatca aaggtttatt ggaaattgtc
     4501 gatcatatac aagactatgc ccccaaagca tggattttga attatacaaa tccagagtcg
     4561 atcgtttctg aagctgttcg tcgtaaatat ccgaatgtaa aaatgattaa tgcctgtgat
     4621 atgactattt caattgaaga aacgattgca gtgaattacg gttatgatcg taagaattgg
     4681 atcgttacgt attatggttt aaaccacttt ggctggtata catcgattta tgataaagaa
     4741 ttgcaacgag aaattatgcc agagattatt gaaaaactaa taacaaaaga aatgcaagtc
     4801 gccgatttta atataggaga taaaacttgg caaaaaacct ttcaaatgat gtcagtcatc
     4861 acgaaaaatt ttccaagtaa tatcccgaat aattacttag agtattactt atatcctgat
     4921 atggtcgttg agcatactga caaagagtat acacgtgcta atatggtcat ggatggacga
     4981 gagaaaaata ccaaagaaat ggctgataaa atacgccgcg gtattgtgga agaagtattg
     5041 aactttaatt ttggggagca tggacaatat atcgtcgata tagctatttc cttgttgaat
     5101 gatgatcgtc gtcgctttat gctaatcgtt cctaatcaag gggcgattcc gaatctacgg
     5161 tcagatgcag ttgttgaaat accggcatat gttggtgcaa ctggtgtgga gccaattaca
     5221 ctaagaaaac caatcagtga ttttcataaa ggattgatgg aggcacaagt tgcagtagag
     5281 aagttactag tagatgctta ctttgaaggt tcttatcaaa aagctttaca agcatttaca
     5341 ttgaatcaaa ccgtaccaaa tgccagagtc gcaaaaaaaa tactagatga aatgatcaaa
     5401 gtaaacaaaa aatattggcc agcattaaag tagaggtgat aaaaagtgca tttctttaaa
     5461 acaagaaaca ttctaaaagc tgtagctagt ggtcaaatta ttccattaac agcagtaaaa
     5521 gacgaggtct tttcttcaaa aatgatagga gttggatacg ctataacgaa tcatgatgga
     5581 tatatccatg cgccagttaa aggaataatc gataatatat ttcctacccg acatgccata
     5641 acgatcgaaa gtgaatctgg cagaaaaata ctgatccata tgggtttaga tacagttgaa
     5701 ctcgaaggaa gtccgtttca gatacgggtt gaaagtaacc aggaggtaaa agaagggaca
     5761 ctgttagcta ttatagattt gaagaagttg gagcaagcac aaaaagatac ggtgatcatt
     5821 gtagtttctc caggcatttt tgaaggaaat ttattaaaac aaaaccaaga agtaaagatt
     5881 aaagatagtt gttttgaatt ttagtgtacg at
//
LOCUS       DAJSMI010000156         5907 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJSMI010000156 DAJSMI010000000
VERSION     DAJSMI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.158"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(368..1486)
                     /locus_tag="ON430_002363"
     CDS             complement(368..1486)
                     /locus_tag="ON430_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220254.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="ON430_002364"
     CDS             1957..3264
                     /locus_tag="ON430_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCR3220255.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="ON430_002365"
     CDS             3383..4582
                     /locus_tag="ON430_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR3220256.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="ON430_002366"
     CDS             complement(4609..5877)
                     /locus_tag="ON430_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCR3220257.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT         1813 a         1251 c         1058 g         1785 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcct
//
LOCUS       DAJSMI010000157         5699 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJSMI010000157 DAJSMI010000000
VERSION     DAJSMI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.160"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..267)
                     /locus_tag="ON430_002367"
     CDS             complement(<1..267)
                     /locus_tag="ON430_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220258.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDD"
     gene            complement(277..1437)
                     /locus_tag="ON430_002368"
     CDS             complement(277..1437)
                     /locus_tag="ON430_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR3220259.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(1437..2123)
                     /locus_tag="ON430_002369"
     CDS             complement(1437..2123)
                     /locus_tag="ON430_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR3220260.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(2086..3264)
                     /locus_tag="ON430_002370"
     CDS             complement(2086..3264)
                     /locus_tag="ON430_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR3220261.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(3284..4978)
                     /locus_tag="ON430_002371"
     CDS             complement(3284..4978)
                     /locus_tag="ON430_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR3220262.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(4956..5270)
                     /locus_tag="ON430_002372"
     CDS             complement(4956..5270)
                     /locus_tag="ON430_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR3220263.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(5373..5654)
                     /locus_tag="ON430_002373"
     CDS             complement(5373..5654)
                     /locus_tag="ON430_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220264.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
BASE COUNT         1651 a         1195 c          816 g         2037 t
ORIGIN      
        1 gtcgtcctca cgcgaatgat cttcttctaa atatttccac cataaatatg gtttaatcga
       61 aggaatatac tctttgtcgt cccgcagatc atcgtacaca tgaacttcaa caccatattc
      121 cttctcaaaa aaaataggaa tctgatcatc gtgtctgctg aaaagcaata cgatatcttt
      181 gattcctagt ttcttcagat tagttaaaca aacttcaagc tcccatttaa accgattgat
      241 tgccggctga caaagaatat acttcattct gatcacctac gcttgtgttg tagttgttgt
      301 ggttgttggt tttgtagttg tagtagtagt tcccaaagcg ctaatatcta atacaatgaa
      361 actatcgtta cgtttaggtt gaccgtttgc atattgttta gctagataaa tgcgttcgtc
      421 ttcaacaaaa tggtattcat ctgaagcttc aatttttagt gtagatccta cacccatgaa
      481 gtaatctgag gctaccccaa taactgcttt tccttctggc acagccgttg actgcaaatc
      541 tgaaactggt actggcaata cttgtacgta ttctccatta gcagttagta cagtcttagc
      601 tgggaatact ttagaccagt aatcagttgg attcacaatt aggaccacat cagaaggatt
      661 cacattacga taaatcggat cattcacacc ttcgatattg aattttgata gtcgcgccat
      721 caaaccgccc atagttacag catctaaagc tgtaataggt tctgcttttt tttcagcata
      781 ttctccgcta gtttgtttgc tcatgtcacg catcattccg actggcatat ctttaccagt
      841 accatcaaca attgcttgtt ctaatgcaat tctcaatgat tctactaaaa cagtacggac
      901 ataacgatct aaccatactg gacctaaatc aagcattgcc ttacatacag gaatataacc
      961 tgatagcttg aactgcttca tgttaattac atcaaagcca ttatctaaaa cttttttaac
     1021 agcttcgcaa agtttacccc accatgctgg attgactcca cgtgacacaa tccattctgt
     1081 tacaccagtt gtgttaacaa aagtaatttt ttgcaatagt ggatgagatt gttctaaatc
     1141 ttcaaataca cgttcaaata cagtagctgg cactaattct tcgacccctg caaaaccttc
     1201 gtttttcact acttcgttat agaattttgt ttcttgtgta gttaatacac gctgaccacg
     1261 gttcattaat actaattgat cttgattttt tgctgttgct tcttctaaaa ttttatcctg
     1321 aatttcctta gataagctta ccatagctgc gctaaaagat tcttcgttac catctttaaa
     1381 agctttcatc aattggtcgc ttgcagctgt tacacctttt aaatttttaa ctgtcattat
     1441 tttacatctc cttgtccaaa tgttttattt aatgctgctg taaatgcagc aattttttct
     1501 gctctttttt ctttaacgtc attcaaaatt tcttcaacgc tttgttcttt tttagcttca
     1561 gtacctgagc tattttctgc atcgataatt tcatcgacca atccataact caaagctgtt
     1621 tctgcatcca taaacgattc tttttcaaga agttcttgca atgcttcatc tgtgccattg
     1681 aatcgtgttt tatatgaagc ctttaccgat ttatcaattg attccagttg gtcagcaatc
     1741 gtacggaagt catcgacatt tccttctccg tatgtggaag cgcggtgaat catcaattgt
     1801 gcattgttgt agatttttat agtatcgcca gccattgcga taattgaagc agcactagcg
     1861 gctaagccgt taatcacaac gttaactttt gctttatttg acttaagtaa gttcccaata
     1921 gcaatccctt gaaatacgtc tccaccgttt gaattaatta ctacttcaat ttcttcttga
     1981 tcacctagac tatccaaaat atttttgatt cccttgtcag tattcccttc aaagaaccaa
     2041 ctagaaccaa taaatccctg aataaaaatt tgcggtactg cgccttcatt ctttactgct
     2101 agaaatgttt tcattgtcgt cattcgcctc acctcctttc gatacttgtt gattgttttt
     2161 agttataaat atttcatctg ccatcgcctt atcagagcga tcatttccaa cgcgttctct
     2221 tccttcgttg attgtaaata ctccatttct aatgcctaca tcaatagcgt caaccaaatc
     2281 tttgaagcta gtaatcttga tcatagttgt atccacacgt acaaaattcc ctgacaagta
     2341 ttcttctact tcatagagac tagcgttaaa cgcatcctga ataagttcag caatcggtat
     2401 gatttcgaac attaaaaaag cgtccacttg atccgataac ccactcatgt ctccctttag
     2461 taggtttttc ggaacgtgaa acgctgctgc tgtcatctca aagatgtcgt ctattaagtt
     2521 ttttatatct cttgaattgc tttggaagtt tccgctgaaa tcttctaatg tgtactcatt
     2581 ttgtaattga aataccgcac ctgcattatc agcttccata aaagccttaa attgtgatgt
     2641 catcatttta ttgatttgat cttgtgttgt attgtcttgc ggtcggaata aattcccttt
     2701 cagtacgtat ctacgagcgt tagagcgctt gtaaacattc atggcactag aaatgagttt
     2761 cccatacgct tgataatacg catcgactag ttgcctaatt tgttgatctg cgtattttat
     2821 atagataaca tcactttcta gaaattctct atcaaggact atgttgttaa tttgcacttg
     2881 agaaaacaca tcatctttca atgcatattc tgtgacatcc caactatccg caataaatat
     2941 ttcgctagaa ttattagacg gagaaacgat caatacttca ttgtagaata ttaatctcct
     3001 gatcagtttt tttctaaatt ctgttgcatt atttttctta ttaggagcta cattcagcct
     3061 atagtaaaga tcattctttt tattttttcc atcttcatat gacttgaatt ccgctttact
     3121 catcgcattt gcaatcaaat caatacaagt ttcaatcgca aattttcgat acacaaaatc
     3181 aacttgcaat ttacaaaagt attcttctaa aggaaccgtt gctttttttg tgaagtatcc
     3241 taccgccttt tgaaaaatcc ccactttctc acctcctttc aagttagaat actagaggag
     3301 taaatccagt tcctgtattt tctactgagc tatttgtgac tgttacagga gcagaatcat
     3361 aaatatcatc taaaaaattc aaaccatgaa ggaatgaaaa aaagccatcc gtttttctag
     3421 tttcaggttc tattttttca tagcgtatat ttccattaga aatatgctct tcatatacat
     3481 tcatgcaata ccaacgcata atcgcatcgt caccaaaaaa taaacgttga ttaataaaaa
     3541 ggtcatcaac cagatctttt aacataccat gtgtaacaga tccgcttcga acaatttcca
     3601 cagtaaaacc tgcttcttct aaagcgggct tcaatatttt tgcacggtac atatccatag
     3661 cgattttttt aatataatat ttattactca tttcaagaaa ccaacctaca atataatcag
     3721 cttctatatt ttttccatga acgatctgtg attttccttg atctatagaa atatctataa
     3781 cctctcgttt gatgttttgt aatcgaaggg ctgattcgtg gataaaagta tgttgtgtaa
     3841 aataaacatc tttatcgtat tttcctagca acccaacgct ggcaaaatct cgtctatcag
     3901 caaaatcgac tgttcctatc acttcatcca ttttttcagg aaattctttt tctttcgtat
     3961 gcagaacatc atcatatgaa gcaacagcaa atcgtgtatc ttccataggt ctgttcattc
     4021 gtttggtcat gaacgtaagt cttaaaccag cattacgttg catttgagag tattcttgaa
     4081 acattttccg ttttaaatct gcattgtaat taatagttgg acaagctttt tcccacatgt
     4141 cgggatcatc aacttcatta tcgttatcca agcgacaaat aaatggaaac aaactagaaa
     4201 attctgctcc atccttgtca attccaagtt ctccagaaag aatcattttt gattcttcta
     4261 taatgtcatc aagcggacca ccacgaacat gaccattagt tgtatcataa aattctctat
     4321 aatctcgaat tttaccacca ccagaagtag ccacatttat cattgaataa tcttcatttt
     4381 cgtgaatttc atcaaagcgg tttgcacctg gtcgcttccc atcttttgtt ctagcatttg
     4441 ccgtgttata acgaagtttg ctgtttgtag cgatattttg aataacttcc ttcgtagctt
     4501 taaatacttt tttatctaaa tcaggatgat ctttaattac tttaaataca tcatcaaaac
     4561 tagtctttgc ttggctttca ttattggcat agatatcaat atcataattt ttaataccgt
     4621 gtttagcggt tagtagaaag aagttgttcc aagaagcaaa accagtttta ccattaccac
     4681 gtcccattaa tgaaagatat ctattgaaca ctagtgtttt atcttttttc catcgaacac
     4741 cataaataaa acattgtaga aatttttccc acggaattaa ttcgaatgga aagtattgtg
     4801 ctggtatatt gattgaatcc tctaccatct gcttatcgaa gtaaatatct tctctagtaa
     4861 agactctttc ttctagataa ttttttagca ataattgctc tttgcatacc ttgatagtgc
     4921 cttcttctat agctttgaac caattttcaa tatgcttata actcaggaat tgattcattt
     4981 gcttcaccta ccaattcagg agtaatggca agtttatcca acatcaatcc catttgtttg
     5041 ttgacagaaa caagcaacgc tactgattca ttctttttac cattctccag tctaatgccg
     5101 ttctcggata tatcttcttc cagtgatatc gccgtttccc ataaactgat ataacgatca
     5161 acattatcta agaatggctc aatatttgtt ttctgacttt ccaattggct tattaaagag
     5221 cggcgtaatt tttctctgta gcgattttga gacaattcgt ttttaaacat tttagccctc
     5281 ctttcatgat aaagttcgaa aaaatctctt ttcctgacag ccccctccgt ttcatcaccc
     5341 ccaaaaaatt tgcgatttat tttaaggggg ggttatctca ccatcggaat gaaagcttca
     5401 gcgaagtcaa tgtaataatt aatctcttca atactatatc caaaaacatt ttttattctt
     5461 tcgacgttat tatctttatt caacgcttct cttacttgat tcactttgta tttactacaa
     5521 cagttatctg ataacagatc cctaatacct acatagcgaa cgtatatcaa acgtttaatt
     5581 aatccctgag tataagatga atactcttca atcttttctg tatcatactc tctgccatta
     5641 tcattgatga tcatgcactt accacctttc acttgcatcg aagttagcaa agctttcta
//
LOCUS       DAJSMI010000158         5676 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJSMI010000158 DAJSMI010000000
VERSION     DAJSMI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.161"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(29..1960)
                     /locus_tag="ON430_002374"
     CDS             complement(29..1960)
                     /locus_tag="ON430_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3220265.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1978..2892)
                     /gene="pfkB"
                     /locus_tag="ON430_002375"
     CDS             complement(1978..2892)
                     /gene="pfkB"
                     /locus_tag="ON430_002375"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR3220266.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2889..3641)
                     /locus_tag="ON430_002376"
     CDS             complement(2889..3641)
                     /locus_tag="ON430_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3220267.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3801..4010)
                     /locus_tag="ON430_002377"
     CDS             complement(3801..4010)
                     /locus_tag="ON430_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220268.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4021..5349)
                     /locus_tag="ON430_002378"
     CDS             complement(4021..5349)
                     /locus_tag="ON430_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220269.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5485..5640
                     /locus_tag="ON430_002379"
     CDS             5485..5640
                     /locus_tag="ON430_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220270.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT         1648 a         1205 c          983 g         1840 t
ORIGIN      
        1 ggaaaaccca gcctcttctt ttttatttct atgcttctgc tactgcagga gcttttctca
       61 ataaaccata aatcacacca gatacgactg ctccgatagc aataaacacc aaatacatga
      121 ccgggtgact cagtaagaat acaacgaaaa tcccgccgtg aggagccagt aatttgattc
      181 cgaaagctcc gactaatccg ccagttaaag cagaaccaat aataaaactt ggaatggcac
      241 gaatcgggtc agctgctgca aatggaatcg atccttccgt gacaaacgat agtcccatca
      301 cgatgttcgt caaaccagca tcttgatctg ttttgctgaa tttattctta aacaaacgtg
      361 tagcaacgaa aattgccaat ggaggtacca ttcctccagc cataacagaa gccatcacga
      421 cacttccgcc agttgcaaca gtcgtagcta aagttgctgt accaaacaca taagcggctt
      481 tgttgattgg accgcctaaa tctgccgcca tcattccagc taataatgca ccaagtaaag
      541 cagcattcga accactcata cttgtcaaga aaccgttcaa tgcatcattg attgctttca
      601 tagggacatt gaccagcaac atcaagaaac cagtaatcag taagccaaac actggataaa
      661 agaggatcgt tttaattcca tccaatgatt ttggtaagtt cttgaataat ttacgtaaaa
      721 atacgatgac ataaccagct aagaatccac caatcagtgc acctaagaat cctgcaccgc
      781 ctgtattagc taatgcacca gcagcaaatc caacgatcaa accagggcgg tcaccgatac
      841 ttgaagcaat aaatcctgct aataccggca gcatgaaacc aaatgcagct tgtccgattt
      901 ggttgaacca gctagcagct tgattgtaat tccctagatt tgataattga tcatgtggca
      961 cgccaataaa ttggtcgatc atgaaagaaa gcgcaatagc gattcctcca ccaataacga
     1021 aaggtaacat gtgtgatacg ccgttcatca aatctttgta aattctttgt ccgatagtac
     1081 catccgcatt tccttcttct gaactgcttt cttttccatc accatggaat actggtgcag
     1141 taccactaag ggctagattg atcaactctt ctggtttacg gataccatca ctgacaggac
     1201 gattgatcag ttcttttccg tcaaagcgat tcatctctac ttttttgtct gctgcaacga
     1261 ttacaccttc tgcacgggcg atgtcttcag cagtcaatcg gtttttgatt ccttctgaac
     1321 cgttcgtttc gactttgatc tccacaccca tttctttcgc ttttttcttc aaagcatctt
     1381 ctgccatgta agtgtgagcg atccctgttg gacaagctgt aacggcaaca acgaattttc
     1441 tttctggatg agcagaagct tctgcagctt gtgtttcttg ttctgctttt tcttctgctt
     1501 ctttttgctc ttcagctgtt tggaacaagt cttggacttc ttctggtgta tttgcttctt
     1561 ttaattttcc tacgaaatct gcatcaatca gcaatcgaga tagtgctgct aatgcttgca
     1621 aatgtgtgtc gtttgcgcct tctggagctg cgatcatgaa aaacagataa gttggctgtc
     1681 cgtctaaggc ttcataatct actccttttt ggctttttgc gaataaaact gttgcttctt
     1741 taactgcact gtttttcgcg tgaggcatgg cgattccatc accaagacct gtagaagttt
     1801 gtgcttcgcg ggctagaatt ccttctttat atgtttcgat atcggaaatc cggccggcat
     1861 caaacatttt ttggaccatt tcatcgattg cgcctttttt atcagtcgct tgcaaatcca
     1921 tgatcatcgc ttctttgacc attaaatcct tgatattcat gattatcctc cttgaaacta
     1981 atcaattttt gtgatttcaa cttctggcag caactcatca atcaattcgc gagctgccaa
     2041 gtcatcagaa aaagctgttg cgcttccgca agcaactccc catttaaagg cttcgattgg
     2101 atctttcgta cggctgtaag ccccaataaa gccagcaatc attgaatccc ctgcacctac
     2161 tgaatttttc aatgggcgaa ctaatacatt cgttttataa gttccttgtt ccgtgaagag
     2221 caaagcaccg tctccagcca ttgagatcag cacattctgt gccccttcag ctagtaattt
     2281 tttcccatac ggaatgattt cttctacaga ttggaaggtt gtgttgtaaa gctcagctaa
     2341 ttcgtgattg tttggtttga tcaacaacgg tttcttaaca agtgcatttt ttaggtcatc
     2401 acctgttgta tcaatcacga aatctgcacc tttttctcga atgatatcaa tcagttcttg
     2461 ataaaatcct tttcgcaggc tagctggcgc gcttcctgac aatacgacga tatctccttg
     2521 tcctactgtt cctaatgctt gcttcaactc atcgatttct tcatctgaaa tagatgggcc
     2581 taagccattg atctcagttt cagtgtctga ttttagtttg atattgattc ttgtatcttc
     2641 ttttacggtt gtgaacaaag actggatttg ttctttttca agccacttcg aaataaattc
     2701 gccagtgaac cctcctaaaa accctagtgc tgtagattga gaatcgatcc gtttcaaaat
     2761 acgggaaaca ttgatccctt ttcctccagg taacttgaag tcttccttca ttcggttcaa
     2821 atcgcctagt tttaacccat ctacgcgtac gatataatca attgatggat taagtgttac
     2881 tgtgtaaatc attgtattgc ctccttaatt gttgtctttt cacggaattc attaacgaat
     2941 tctaatggac aacgctcggt tatgatcgtg gctgcttcca aaggtgcaac agaggtgaag
     3001 gacaactggt tgaatttggt atgatcaatc aatacaaatg tctcttcgga atttttcatg
     3061 gctaaacgtt taacagctgc ctcttctgga tccggagtcg tgaatccttg ttcaagatgg
     3121 gcaccgttca ttcccataaa tgctttatta aaatgaaaat tttttagttg ttctatcgca
     3181 ttagatccta aaacagcatt agttgccagc tttatcgttc cacctaaaat aatggtagca
     3241 agctccaaat caactaaatt tgccgcatgt tgaacggaat ttgtcactac tgtgatctgt
     3301 tttccttgca gaaaaggaat catttctaac gtagtagagc cagcgtctaa ataaatgaca
     3361 tcttcgtcat taatggtttc agctgccgat ttggcgattt gctgtttttc gtgaacgttt
     3421 ttgagtgatt tttcactcat attctgctca aaattcaata attgctcttt cttagctccg
     3481 ccatgaactc tttctagaaa cccactattc tccagctctt gtaaatcacg tcttatcgtt
     3541 gattcagaag cattcaaaag gtttacaaga tcttgtgatt tcacgatttt ctgctgttca
     3601 agcaagtcta ggattttttg tctgcgttcc tctgtaagca ttttcacgat cacctcaata
     3661 caaataatag catatttttt ttctactagc aatataaatc agtcaaaaac gttcataaaa
     3721 gttataaatc agtcaaaaaa aaaataaaaa accagtcaga aagactggtt ttttattttt
     3781 ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt tttcatataa
     3841 acagaagcag gttttctttc cttatgaaga agataataat aaagtcccat cccgccaaac
     3901 atgaccaaaa aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa gagacagacg
     3961 gataataatg atgccggaac cattattcga taatagagtt gtttatccat tttcttcacc
     4021 tcatattttt tgtaaagtaa tcaattgttc taattctcgg taatctaaaa attgcacacg
     4081 cagatgctgc tcacctttct tgacatcgaa ttggatactt cgccgctttt tctttattcg
     4141 tttgaaggta acatcagaga gtctatgttt gatttccttt aacggtaatt ctgacaggga
     4201 ttctgaagga acatagattc ttaaggataa aagatactct tgatattttt catctgttac
     4261 ctctacttct agatattccc taatataata acaataagct tcgattccat tatccaaagc
     4321 aacagtcgga tcgataactt tatagcacat catgcgtgat cgattctcgt caacccaagg
     4381 aaaatacgcc tgagcattta gtgttccgcg aacacgcctt tctttataat ctccttgttg
     4441 gatatcataa ctttttgtta caaaaaatga agcattcttt ttaggaacag gttgcatttg
     4501 tgcattctga tactggacag attggatcgt atggtcagtg taaatgtatt caacgaatgg
     4561 cggtttcaat aaaccgacac cattgtccca agcaagatga tcaaacccaa gctggtccct
     4621 aaaataggat tgaaacattt gattgtttgg cttttgccag ttaaaggggt ctcgataaag
     4681 ctggatacat atgacttcgt tttctaattc cttcactttt tctacttgtt ggatgtcgag
     4741 aaaaatcggt ttagggatga tttgatgata atagcgacta taatacattt cccaacacga
     4801 agtgaaacat aaaccttggt aaaaaagatc gtatcctgga aattggttgc aatcgactac
     4861 tacttccccg ttttgttgtt tcattttagg taaatgttcg atttcatcct ttttctcaaa
     4921 caatagccgt tgagagatct gttttgtatt gtgatagata aactcgctat aagctcgcat
     4981 ataggcgaac accccatgag caatcatttt tttctgaatg aagtctcgaa tcaaaaattg
     5041 cttttctttg aaaacagaga tagacaatac attttttatc aataattgat tatcttccaa
     5101 gcgaaaacta cattcgttct cgtcatttcc tacaactaac caactttcgt ctcttaccga
     5161 tgctagcatt attttttctt gaagctttcg aagatcatac ggatccgctt gttgtgtgaa
     5221 agagcggata aatgttggct ggatcaacag acgtgtcaaa atagcccaat cgcttatttc
     5281 ctgttttttt tcttcttcat ttaagtctgt gagctggata gtcatcaata atttatcttc
     5341 gccaatcatt ttctcacctc tttcaaatct ttttataatt taattataac caatgataaa
     5401 gaaaaactaa aactttatgt cttagaaacc aatttggacg attacatgat agactaagta
     5461 aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga taatttagat
     5521 aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa agaagagatc
     5581 gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa acaaaagtaa
     5641 gttaagctca aaaaaagact taccagccaa actttt
//
LOCUS       DAJSMI010000159         5664 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJSMI010000159 DAJSMI010000000
VERSION     DAJSMI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.162"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..194)
                     /locus_tag="ON430_002380"
     CDS             complement(<1..194)
                     /locus_tag="ON430_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220271.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
     gene            complement(737..2887)
                     /locus_tag="ON430_002381"
                     /pseudo
     CDS             complement(737..2887)
                     /locus_tag="ON430_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3033..3608
                     /locus_tag="ON430_002382"
     CDS             3033..3608
                     /locus_tag="ON430_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3220272.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3822..4517
                     /gene="vanR"
                     /locus_tag="ON430_002383"
     CDS             3822..4517
                     /gene="vanR"
                     /locus_tag="ON430_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR3220273.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4495..5649
                     /locus_tag="ON430_002384"
     CDS             4495..5649
                     /locus_tag="ON430_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR3220274.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1770 a         1092 c         1073 g         1729 t
ORIGIN      
        1 tgttcaaaat ctaaaatcaa cacatcatgg ggagtgtgat aataaatatt ttcccttaat
       61 gtaccaacaa tggtaattcg gtcaattgac cattgcattt ttaagggggt gcacatagtt
      121 tgtgcattta accgcctgtt agaaatacgg ttaagttttt cccactcatt tacgattatg
      181 ccttttttac tcaaaaatag ctcctcctca acgaaaaaag agattcttgg aaagaatttc
      241 cagagttcac gtaactctaa aaatccacat ccaaaaatct ctagcttgaa gaaacctaaa
      301 aatattcgta taatgaactt gagttacgga actaggtgaa gtagtttccg taaaggtcga
      361 gagtgcgtca acactgctcg actatttttt tactcttttt tctcattttc tttgattata
      421 gcactcctct agaaaaaata aaaagacctc agggaatttc attccccgaa ccccttgagc
      481 ctcctcctgc cgaaatagaa tggcgctgcc agcatttatt tcgttcagct atcggctaca
      541 caatctaaca cgaccgttcc gctaccagtc gccttacagg ctccattttc gctcctctaa
      601 cgtgtttcac cgattgtgcc gataactaaa cgaaataaac gctaaaaccg ctcagaacgc
      661 aaaatatgcc attttagagc tattgtagaa aatatagttt gaaatgcaat tttcttcttt
      721 cagcttttta tttcccactt gtaaatcggt agatggtggc tccttttcca gttccataat
      781 gtggatttgc atctgcatgt agtgatgaaa cacctagctg cattctcata ccatctgacg
      841 aagatgttgt accgtcgccc caatagaaag gcaattgtaa tttatgatga aagtttacta
      901 atatggcttg ggctttattc atggcatctt catacatgcg ccattgagat acattggcta
      961 gttgcttata tgtaagtccg ggtgtggctt cggccatctt gctcaagcca atattcattc
     1021 ccattcctaa aagggcagcc atgataatga ttgtttcttc cttatctggt tttcgattat
     1081 tggaagcatg agtgaattgc tcatgaaatc ctgttatatg ggccacatcc atgagtaaat
     1141 cagttaattt tattcttggt agcatctgat aaaggcttgc actaaatttt tttgcttctt
     1201 ctggaacatc tttttctaag cgtgcaagtg atagctttcc tttttcaaga gaaaccccat
     1261 ctaacttatt ggaattggca gctaaccact ttaacctttc attaaagctg ctggttctct
     1321 ccgttatata atcttcgaat gataaactaa ctgataatct cgtattcccc ttcgattgat
     1381 tccatgtatc ttccgaaaac aaatattcct caaaatccct atattgtctg ctgccaacaa
     1441 tggaaacatc tcctgcccga acatgctccc gaagttctgt taaaacagcc atttcatagt
     1501 aatgacgatt aattgttgta ccatcatcct cgtataaatg tcttttccat cgttttgaaa
     1561 taaaatccac aggtgagtca tcaggcactt ttcgctttcc agattcgttc attcctcgga
     1621 taatctcaac agcttgtaaa agtggctcat ttgcctttgt agaatgaaat tccaatactc
     1681 ttaatagcgt tggcgtatat tttcttagtg aataaaaccg tttttgcagt aagtctaaat
     1741 aatcatagtc ggcaggacgt gcaagttcct gagcctcttc tactgaagag acaaaggtat
     1801 tccattcaat aaccgattct aaaaccttaa aaacgtctaa tttttcctct cttgctttaa
     1861 ttaatgcttg tccgatgttc gtaaagtgta taactttctc atttagcttt ttaccgtttt
     1921 gtttctggat ttcctcttga gccttacgac cttttgataa caaactaagt atttgcctat
     1981 catgaatttc aaacgcttta tccgttagct cctgagtaag ttgtaataaa tagatggtta
     2041 atatcgaata acgtttattt tcttgaaagt cacggaatgc atacggctcg tatcttgagc
     2101 ctaagcgaga cagctgcaac aggcggttac ggtgcaaatg actaatttgc actgtttcta
     2161 aatccattcc tcgtatgtat tcgagtcgtt ctattatttt tagaaaagtt tcgggtgaag
     2221 gatgacccgg tggctctttt aaccaaccca atatcgtttt attggattcg gatggatgct
     2281 gcgaggtaat aatcccttca agcttttctt tttgctcatt tgttagagat ttactaaccg
     2341 tattaaatag cttcttttca gccattgccc ttgcttccca caccattctt tcaagtgtag
     2401 tgatagcagg cagtataatt ttgttttttc ttagaaaatc tatgcattca tgcagtagat
     2461 gaatggcatc accattttcc aaagctaatt gatgaaggta cttaaatgtc attcgatatt
     2521 cactcagggt aaaagttaca aagtcgtatt cacttcgaat ttctttcaaa tgatcccaaa
     2581 gtgtattttc cctttgagga taatgatcaa gcgaggatgg actaacacca atctgtttcg
     2641 atatatattg tatgaccgaa tctgggatgc ttttgatatg agtgtatggc caaccgggat
     2701 accgaagaac agctaattga acagcaaatc ctaaacggtt ttcttccctc cttcgcttat
     2761 taactatttc taaatcccgt ttggaaaaag tgaagtaggt ccccagtatc cattcatctt
     2821 cagggatttg cataaaagcc tgtctctgtt ccggtgtaag caattctcta cctctcgcaa
     2881 ttttcattca gtatcattcc atttctgtat tttcaattta ttagttcaat tatatatcaa
     2941 tagagtgtac tctattgata caaatgtagt agactgataa aatcatagtt aagagcgtct
     3001 cataagactt gtctcaaaaa tgaggtgata ttttgcggaa aatcggttat attcgtgtca
     3061 gttcgactaa ccagaatcct tcaagacaat ttcagcagtt gaacgagatc ggaatggata
     3121 ttatatatga agagaaagtt tcaggagcaa caaaggatcg cgagcaactt caaaaagtgt
     3181 tagacgattt acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta
     3241 gtacacaaga tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat
     3301 cactaaaaga tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta
     3361 ctgtaatggc tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag
     3421 ggattgaatt ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa
     3481 atcacgcagg aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc
     3541 aaatttgtga aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga
     3601 ataattagcc attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta
     3661 taaaatatta acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca
     3721 tcttaagaaa ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg
     3781 ataaattatt aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta
     3841 ttgtggatga tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt
     3901 atacggtttt caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga
     3961 ttgaccttgc catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa
     4021 aaataaggga caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag
     4081 ataaaattac agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac
     4141 tggagttaat tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa
     4201 aggagcagaa cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg
     4261 agtgttatct gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa
     4321 tcctctgtga aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg
     4381 gcgacgaata tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg
     4441 aaaaaatgaa cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt
     4501 ataaaattga aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat
     4561 atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga
     4621 gggaaacttg gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg
     4681 gacgcgatga aattatatca atattccata cggaacaata tagatatctt tatttatgtg
     4741 gcgattgtca ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac
     4801 tttgacgaga taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag
     4861 ctttctgcgg aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa
     4921 aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg
     4981 gcgcacgata ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag
     5041 gctccagaca tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg
     5101 tatcgactcg aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg
     5161 ataacgctaa caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa
     5221 ttttatcctc agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg
     5281 accgtgtccg gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc
     5341 gctgcataca gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg
     5401 gtgtcaatcg aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt
     5461 gaaaagttct ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga
     5521 ttggcgattg caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat
     5581 gataactata cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg
     5641 aggtcctaag agatgtatat aatt
//
LOCUS       DAJSMI010000160         5614 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJSMI010000160 DAJSMI010000000
VERSION     DAJSMI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.163"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..1610)
                     /locus_tag="ON430_002385"
     CDS             complement(<1..1610)
                     /locus_tag="ON430_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR3220275.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQD"
     gene            complement(1773..2366)
                     /locus_tag="ON430_002386"
     CDS             complement(1773..2366)
                     /locus_tag="ON430_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220276.1"
                     /translation="MRVKGQNSVDRKSILQFGKPEERSILDLFPYKSCTEEGVLITKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(2367..2699)
                     /locus_tag="ON430_002387"
     CDS             complement(2367..2699)
                     /locus_tag="ON430_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220277.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTIGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(2793..3263)
                     /locus_tag="ON430_002388"
                     /pseudo
     CDS             complement(2793..3263)
                     /locus_tag="ON430_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3324..>5614)
                     /locus_tag="ON430_002389"
     CDS             complement(3324..>5614)
                     /locus_tag="ON430_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220278.1"
                     /translation="PKTVTKVLQDYGNYLQYQNVFSWIYNTFLWGLIKGLYNLNTLLE
                     KTIYQSFDLKDLLNAAGVNELFQSLISKVLALFCVVTLIYLGLKFFMSKHPPKIKNIL
                     VNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAANKADKTGSPAFQIIQNNVIDVSTL
                     LENDPNKVEHIPTDKRNALTAKNFKTANINGIITPDQAKKMSESSDISTVQKDRLKAL
                     QYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRYISSPGIILAGESSLSVAYLFILFT
                     TVTCIIELVFKKFYLVIAASTDFETGQRMKSAIEDISQSFLLLAFTILELRIYTLMLS
                     GIGDLHAAGKINGFLYVVALIVLTIALFKGSQSVTKIFGVDTSLKNGSNSLLSMFALG
                     NIAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGNRKDVTPDNGEEQSDDKATTVPNSK
                     KTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAEDLTEKAKDTLEEKLDGTRAKELAE
                     AVKNPSETLEKQKEKASEVKEGLKDAYGEGQVNAAVKANTTTEKENQEGAAIPSAKDH
                     QDELHSNENSEEGQNGQLPKTDPEQYRYKSNINSENEKRISDLPKRNQEVQTDDDSID
                     LPNDNGVEVGTEKSTKNGLPKAQNLPMKDSRLNQFKENDQSFVHQSTAGNVQMKPSRL
                     PENGNLTTVSYNLSESGETGNASVQNVVTREAAPEPIQKVHTGYKPANVAFTAPTKIN
                     VTRDTKNRNNALKEQLLKDIKP"
BASE COUNT         1535 a         1147 c          887 g         2045 t
ORIGIN      
        1 aagtcttgtt cttccatttg tttttcttta attaaacgtc gttgtcttct tccttgaatc
       61 aatgcctgat tccaaatcaa cgtcatagcc atgtacattt gacattgttg taccccacga
      121 tctaaacctg taatagagtc aatgtcaaaa aagaccaact gttgttggtc aaggtgtcga
      181 atcgtactat gaccatcaaa aatcatgcca tacgtctcac ataaggattc tacgatggat
      241 tccatttctt cttttcgatt ctttttctct tgggaagcac ctggtaattc cagttgtcct
      301 aaaaagtcta aaaattcttt gaacgtcgga taagcttctg gatctttccc tgtgacttgc
      361 gtctccgtat ttgaacgcga gtttgaggaa gtgaaaccgc gtggtactgt tccatactct
      421 tcataaaaac gatttaaata ggtcttaaat tctgctttca tcgttccttt tagctgagga
      481 ctcactaact gtacctgata gaccactttg tctaagtgcg tttgatagga cgctgcttca
      541 ttgatcttca aatctttgac tgatccatcg tcatataaac tggaagcttc atcatacgta
      601 cgtgtggcaa atatttctaa aggattgatc atcccgtcag atccgtctaa acgcaccata
      661 atgccgttat attcctttaa aaagggaata aagtcttttc ccttttcaaa gccccaaata
      721 aagtctcctg cagctaaatg ggcttccgcg atcattttaa gaacggtcga tttaccagct
      781 cccatcattc ctagaagcaa ggtattggta gacaaccgca cactattttt atacgtggca
      841 ttccaaacaa acgggccgcc tgtcatggtt tccccatatt gtgttccacc tggatctttt
      901 agcgtttggt ggtggaacgg gaaacctccg ccaatgttta gcgatggtag tgattgccct
      961 ttgatatttc caaaccattc agcttgttca tcgaacatgg tgaacatcga ttgccagtct
     1021 cttttcgttt tgtgcagata ggtcactgca cgataatcca tccctttgag attttcgatc
     1081 agttcctgtg ctcgttcatc tacttcttga atcgtgtttc cgtaaacatg tactctcact
     1141 ttgacttgtt tagaaatttc cccattagaa gtcaattttc gggcatattc cgctagttct
     1201 aggtattctt ccgcgctttt atttcggtca gtcgcttttc gttcattatc tgaccgatca
     1261 tttaattcat ctaaggcacg attgatttga tcaatcactt tttgcttttc atctgtgcta
     1321 acatctagtg ttgccactgt atgaggattg ttcatgagat tggccaacca aaaaagcgta
     1381 ggattttttg tgtagtgata aactgtcaca catcgtgaat aaccatttcc ataggtcaca
     1441 tactcttcat cgaaatttaa tccaccttgt ggcatcactt gttttaaaaa gtctaaatca
     1501 taccctttgg attttaaaat ttctatttcc cgatttccta gttgctttaa tggatcaagt
     1561 accttttgat cattttgtgg tgaaaaagct gttttaattg cttctaacat gtacagtcac
     1621 ctcttcttgt ttgattcttt acttggttaa aagattttcc ttttcccaac ttacccaggc
     1681 ttaaagtgtg aggaaatttt ctagtggtct cttctttcgg aacatctttt tctaaccata
     1741 tcaacggata tttcattctc tcttccccat tcctactcat cattcatatt attcattcga
     1801 taaagaatct tgatcaattc ttttttgtag agtggtttta accctaaaat gccactagat
     1861 cgcattaact gacggtttct ggtttgcaaa tccttgattg tctttccttc aatgacgaaa
     1921 aagaagctta aatcagggcg ttcctgttct gctcgtttca attcctggat ttcctctact
     1981 aatttctttt catattttct taatcgctgt agatttcttc ctaaggactg ttccatacga
     2041 tttttatcta ataactgccg cttttgcacg atttgctccg ttagatccgt ttctgtggtc
     2101 aaactaacta acttaatcgt tggcacatac gtacgcatga cagtggtcaa ctgattcatt
     2161 cgttccagtt tctcttgttc atttaatcct tccacatctg tagaaactac acgataataa
     2221 cgttgaaaat gatcttcttt agtaattaag actccctctt ctgtacagga tttataagga
     2281 aataagtcca agatagagcg ctcttctggt tttccaaact gcaaaatgga ttttctgtca
     2341 actgaatttt gacctttgac tcgcatttat tcatcctcct tatacgtacc atgataattt
     2401 cggttaggat ctaacgcatg ataatggttc ttgtccgtaa aaaaaataga ctcgataatc
     2461 gcaaaaatct ggcgttgacc tgggttatta tagggtcgcc aaacaatcac taaagctatc
     2521 gcaatccata agccataatt caagagtgca aaaaagagtg gtaagtggac aagattattg
     2581 actattttcg cccaaaaaaa gcaaccacca atcgttaata gatctaaaaa gaaaaaggcg
     2641 aaaaacttcc attccccact aaatcttggt ggatttttca tatgaatgta tttatccata
     2701 atgtaaccat actccttatg ttctttattt ttagaaacaa aaaacactgt gatttctcac
     2761 agcgtttttc taaatattat aactctaatt caattttctt cttttaattc aaaaattttt
     2821 tgttctaggt ttcttttcat ttgaatcaac tcataatttt gtgcgcgtaa ttcttcgttt
     2881 tccaaatgaa ctcgttgcat ttcatcgttt aatcgattta aattctccaa tgattcttgt
     2941 atcgtttctt gcctaaaatt aagtgagcca agttgacgat ctttttgttg tctttgctct
     3001 ttcaattctg acgatatttg ctcaacagaa tgaaatagct ccgttaattt tactgtgaat
     3061 gtgggcatag catctttcaa ataagtgaat ccttctgaaa ggtgtacaat aatgctttcg
     3121 gtatgtgttt tttgaccatc taaaatagac tgatgatctt tttgtaaact ctccgtatct
     3181 ttaaatcctt taatagtcgc cacaaatatc gttaaaaatg ctcccagtgc agttataaac
     3241 gtagctatta aaatatcctc cacaattttc tcctttccat tttaaacttg ttttataaag
     3301 aaaaaggccc ttaaataggc cttttatggt ttgatatcct tcaatagttg ttctttcaat
     3361 gcattgttcc tgtttttggt atctcttgtc acatttattt ttgtcggtgc agtaaatgcc
     3421 acatttgctg gtttgtaacc tgtgtgaact ttttgaatag gttctggtgc agcttctctt
     3481 gtcacaacat tttgaacgga cgcattccct gtctccccac tttcagataa attgtaagaa
     3541 acagttgtca gatttccatt ttcaggcaaa cgacttggtt tcatttgaac attacctgca
     3601 gttgattggt gaacaaagct ttgatcattc tccttgaatt gattcaaacg actatctttc
     3661 attggtaaat tttgtgcctt aggtaaacca tttttcgttg atttttcagt tccaacttcg
     3721 accccattat catttggcag atcgatggaa tcatcatccg tttgtacttc ttgatttcgt
     3781 tttggtaaat ctgaaatgcg cttttcattt tcagaattaa tgttagattt ataacgatat
     3841 tgttctggat ctgtttttgg taattgacca ttttgtcctt cttctgaatt ttcgttagaa
     3901 tgcaactcat cttggtgatc tttggctgaa ggaatcgctg caccttcttg attttctttt
     3961 tcggttgttg tattagcttt tactgctgca ttgacttgtc cttcaccata cgcatctttt
     4021 agtccttctt tgacttctga agctttttct ttttgttttt ctagtgtttc ggatggattc
     4081 ttcacagctt cagccaattc tttagctctc gtgccgtcta atttctcttc taaggtgtct
     4141 ttggcttttt ctgtaagatc ttcagctgtt ccttttaacc cacgttcatt gacgtaacca
     4201 agaccttctg cggctttaga aacaccattt ttcgctttcc cgaacatttc ggaaactgtt
     4261 ttcttactat taggtacagt tgtagcttta tcgtctgatt gttcttcccc gttatcaggg
     4321 gtaacatctt ttcgattacc aaatccattt ttacgaatat catttaaatt gcttaaacca
     4381 ctcttaccag cacttgcaag gtttttagcc ccgctaccca catttttcgc aatatttcct
     4441 aaggcaaaca tgctaagaag agaattgctg ccgtttttca gactcgtatc aacgccgaaa
     4501 attttcgtaa ctgattgaga gcctttgaat agcgcaatgg ttaatacaat taaagcgaca
     4561 acatacaaga atccattgat ctttcctgcc gcatgcaaat caccgatacc tgaaagcatt
     4621 aatgtataga ttcgtaattc taaaatcgta aatgccaata acaagaagga ttgagaaata
     4681 tcttctatag cacttttcat ccgttgtcct gtctcaaagt ccgttgacgc ggcaatcact
     4741 aaatagaatt ttttaaatac cagctcaata atgcaagtaa cagtcgtaaa caagataaat
     4801 aaataggcta ccgacaaaga agattcacca gctaaaataa tacctggact actaatgtat
     4861 cggcgatagc ctgaagtata cacatatttt gctaaacctt catcactaat ttgaacaggg
     4921 atttctttcc cattatcgtc taactctagt cgatactgta atgcctttaa ccgatctttt
     4981 tgtactgtac taatatcact tgattcagac attttcttag cttgatcagg tgtaataatc
     5041 ccattgatat tcgctgtttt aaaatttttc gccgtgagag catttctttt gtctgttggt
     5101 atgtgttcta ctttgttcgg atcattttct agtaacgtac tcacatcaat cacattattt
     5161 tgaataattt gaaaagcagg tgaaccagtt ttatctgctt tatttgctgc agtcacgtct
     5221 ccgtaaaagt ttttactaat atttagccct tcatcgatta aagaactacc accaaaaatc
     5281 aaaatcatgg ccataaatag attaaccaag atatttttga tttttggtgg atgtttactc
     5341 ataaaaaatt ttaagcctaa ataaattaat gtgacgacac aaaaaagagc caatacctta
     5401 ctaatcaaac tttgaaacag ttcattaaca ccagcggcgt tcaataagtc ttttaaatca
     5461 aagctttgat aaattgtttt ttctagtaaa gtattgagat tatataaccc tttaatcaaa
     5521 ccccataaaa aagtattata aatccaactg aatacatttt gatactgcag ataattacca
     5581 taatcttgta agactttcgt tacggttttg ggat
//
LOCUS       DAJSMI010000161         5600 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJSMI010000161 DAJSMI010000000
VERSION     DAJSMI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.164"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(98..1495)
                     /locus_tag="ON430_002390"
     CDS             complement(98..1495)
                     /locus_tag="ON430_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3220279.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(1527..2498)
                     /locus_tag="ON430_002391"
     CDS             complement(1527..2498)
                     /locus_tag="ON430_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3220280.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2872..3297
                     /locus_tag="ON430_002392"
     CDS             2872..3297
                     /locus_tag="ON430_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220281.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(3785..4486)
                     /locus_tag="ON430_002393"
     CDS             complement(3785..4486)
                     /locus_tag="ON430_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3220282.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(4502..4942)
                     /locus_tag="ON430_002394"
     CDS             complement(4502..4942)
                     /locus_tag="ON430_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR3220283.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(4942..5517)
                     /locus_tag="ON430_002395"
     CDS             complement(4942..5517)
                     /locus_tag="ON430_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCR3220284.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         1602 a         1172 c          892 g         1934 t
ORIGIN      
        1 gacaaaaaag ttgaaaaaga tcgtaaataa ttgaacttct ttttcacaaa atggtaagaa
       61 tggagaattt cacgcatttg ctctcaacta ttctttctta agcctcaaat ccgtttttat
      121 ctgatacgtc tttgaaccaa tgacccgatt ttttgattgt tctttttcca tcttgtgcta
      181 agtctactga aataaagcca tatcgattct tatatgcatt ggtccacgac cagttatcca
      241 tacatgtcca catatgatag cctaaacaat tggcaccttc ttgaatggct tgatggacat
      301 atttcagatg agattggaca aattcgatcc gataatcatc gtggatcatg ccatcttctt
      361 tgataaatcg tgcttcgttt tctacaccca taccgttttc agaaataaag cagtcgatat
      421 ttccataatg atctcgtaca ttgatcaaaa tatcatagat tccttgttca tagatctccc
      481 atccgcgata aggattcatt ttacgaccag gcatttcata attgtcgaaa taatcctcag
      541 gcattggacc gtttttagta tcgattggtg tttcttttgc ttttacccgg cgtggctggt
      601 aataattgat acctaaaaga tctactgtat tttctttgat aaccgtcaaa tcctctgcat
      661 ggatcacagg catgagatca agttctttta ctatctcaat caattcactt ggaaactcgc
      721 cttttacagc tggatcaagg aatgaacgat tgaagaacgc atctgctatt tgcgctgctt
      781 taacatcttc agggtttgat tcgtctcttg gatagcttgg tgtcagattc aagatgatac
      841 caataacacc accttgctcc atttcatggt aagatttgat ggctaacgct gaagccaacg
      901 cttcatgata gccaacttgg atcgcttttt tcatatctga ctcttctgga taatgccatc
      961 gatataaata acctgcttcg actggaacga ttggttcatt atgagtaaac cattttttta
     1021 ctttcgaacc aaacaattca aaacaacttt tggcatattt ttcataggct tcaaccgttt
     1081 ctcggttcaa ccatccgcct ttttcttgca gatacatcgg catatcgaaa tgatacagat
     1141 tcacaaaggg ttcgataccg ttttcaatca actcatcaaa ataacgatga tagaaacgaa
     1201 cggcttcttc gttgattgtt ccagattcat caggtatcaa acggctccat tggatagatg
     1261 ttcgaaatga attatgccca gtctgtttca tcaactgtac atcctcttga tacttcgtat
     1321 aaacttgcga ggttttttca ggtcctactt gatcaaagaa cttttgcggt tcttttttgt
     1381 accaataatc aaaaatgttc tctccttttc catcgcctga aatccttcct tctgtttgtg
     1441 ggccacttgc tgcagaaccc caccaaaaat ttgctggaaa ttgatatttt ttcatcaaag
     1501 cacctctttt tcttttttag ttcgttttag ttgattccat gtatgatatt ttccatcaca
     1561 atcacttttt cattttcttc ctcttctttt ttgattcgtt ttaacagctg ttctgctgct
     1621 cgttccccga ttttatctgt tggtggcgtg atcgtgattt tcggaaggat actcagatcg
     1681 tttttatcta aataatcata gtgactgaac gcaatatcct tcccgattct gacaccttct
     1741 tgattgaaac attcgatggc accaatcgac atattgtaat tgctgacaaa aatagcagtt
     1801 gccccttttt ctaataatgc ttttgccccg taatagccgc tttttctaga atagtctcct
     1861 gtaaagacct ctaattggat cgatggatca ttttggaccg cttctttggc tcctgctaaa
     1921 cgttctcttg ctacgtagtc tgtttgagtg gcagcaataa atccgatttt tttatgtcct
     1981 tgttcaccat gatgagcgat cacttttgct acactttctc gatcattcac taatattgta
     2041 tcaatacctg ctggtccttc tggacaattc aatgcgataa ctggaatact catttttgtg
     2101 agcatttcaa atccagacca ctcttcttcg gataagaaga caatcagccc atcgatcaca
     2161 tgcttcatca ggtattcaat tttttgttca attttttctg gctgatcggc aaacccgctt
     2221 aataataagc tatacccatt ttcttcagct actctttcga tccctgagac aacttccgac
     2281 ccaaaacggc tggaaatccc gtttatcaaa agtccaatag agaagcttcg attattcttc
     2341 aaccctttag caatgatgtt ttctttatag tccaacgctg aaatcgcttc tgctattttt
     2401 ttcatgttct tttccttcag ttgctgacca ttcaagtaac gggaaactgt tcccactgaa
     2461 acaccagcca tcttagccac atcacggatc gatgccatac attctcactc ctgccagtta
     2521 catttgattt ctctttcttg ttgttcgatt atgatgaccg cattctttgc atagtgttgc
     2581 ttgtcatcaa tatccatata actaaagtct tcagtagagc aacttcctct cactattaat
     2641 tatacttaaa caagtatttg aaccggttca tttttaatat agcatttttt taaaagattg
     2701 caacactttt ttaaaacgct ttctgaaagt ggcggatttt ttgccttttt aaaattttat
     2761 tctaaaattt actctatttg tgggtgtttt gtgcgttttt ctcttccatc cgtttgaagt
     2821 tttacattcc aatggtataa tttctttatc ctatcaaatg ggggaagtat tttgaagaaa
     2881 ttaatcgtac tatttatttt gtctgcggga cttttgacat tgactggctg tgatttgttt
     2941 ggcggctctg accaatcctc ggacgaaaca accgattctt ctgtcttaaa agcggaaaaa
     3001 gcagatacca ccaacagtgt atatacgcta aaagtacgta aagatatgga tttatccgcg
     3061 ggacagttga catttacaga taaaaaaatg gaatggacac gcacgtatca gacacaagga
     3121 actgattctt ctgaatcatc tgaagaaaaa acagtgctga cagatatcaa gatcactacc
     3181 aaagatgacg aatatattat ttcaggaaaa gaaaataaaa aagaaacaac agtcatattt
     3241 acaaaaatcg gtaattaccg tttgaaggat gaggatggca acatctacag tctatagtgc
     3301 cgtctgttgg atcttctttt gtttctcccc ttcttttctg ctatagtaaa gaagcaagat
     3361 gattgtaaag gagacgaaat ggatgatcga tagtcctgat tattgtacat gtaataacaa
     3421 caaaaatatc catacagaat ttaatgacca aggaagcttg agcatatgcg acaactgtca
     3481 caaactagtc aaagattcat ttaaaacatt tgaatcttat cctgttcaaa attatgtaaa
     3541 aagtagaaga agataaacaa atccaagcaa aactcactat ggctgatttt tatgaacttc
     3601 aaactcagct tcttcctttg atcaagcttc atcagtaaaa caaacagtgg atcatttcag
     3661 tttacaaatc aaagagtaaa gatttattta tttcaaatat ttgaatataa aataaccaaa
     3721 cgatagaaag gcttattcta catcacgtag taatttttct ttcccgtcgt ttggtttttt
     3781 atttttagcc taattcttga ccttttgcat gctggttatc cgtaaaatat tttccagcag
     3841 gctttccttc caattcagct aatttttctt ggatctgacg gatcaccttt tttgcatctt
     3901 ctggattttc tacaaaatcg aatttatccc catccacttg tattttagga catacatcaa
     3961 aatcttcata ccattgatta tagcgggtgt tcaacgtttc ataataggta tataacgtct
     4021 catcgctttc tagctgttca tagtcacgcc cacgtttttt gatacgttct agcatcgtgt
     4081 ccaaagaaac tttgatatgg accatcaaat cgggccgttt ttttggagca acatcatcta
     4141 actcgtttag catcgtatct aataagctat cgtactgctg tacttcgata tccgttaccc
     4201 gacctaaatc agcgtttaga tggaacaaca ggctatcttc atagatggat cggtctagca
     4261 cattgtcatc gttcactaaa gcatttttca ttgctaaaaa tcgcttgttc aaaaagaaga
     4321 tttgcaataa aaaagcgtac tgctcaggat ttgaataaaa gagcggcaat acttcgttat
     4381 cttcgacatt ttcataaaat ggtttagaat caatctcttt tgcaagcata tccgtcaaac
     4441 tgctcttacc tgcaccaatc gttcctgaaa tcaaaatcac tgacatacaa cccttcctct
     4501 tctataagta ctcgcctacg taagtttcaa caggcaatgt ttcaaaatca tattcacgta
     4561 cagcttggtc tgattttaaa tacgtatgga gactttgtgt aatcatcaag ttcgtaggta
     4621 ccgtctcttc ttgtacaatg atcatcggtt ttttcaacat agttgccaca cctaattcgt
     4681 aagccgttcc tggatcgatt gtttgatgct caaagtcaag gatagcaaca acaaattctg
     4741 catttgtcag ttcttccata tctttttcgt atacttcctg cgcccatcca gcgctgaata
     4801 gttcataatt gctttcttgg tgatgacgtg gagagtagaa acttgtgact gttttgtttt
     4861 cctccaacgc tttttcgata cgacttactc gatcgatttg ctcctctgaa aaaaatggtc
     4921 ctgctaaata tatttgactc attattttgc ctccttcaat aataactgat ttttaataga
     4981 tactgctcgt aattcattga ccagttccaa tggattgttg gaaaagatta cttgatccaa
     5041 ccatttttta tccataaaat cctcttttgc aaaagtttct aataattgtt gcaagccgct
     5101 gtaataaccg ccaatattca atattccgat tggcttgtcg ggcgtcagac cgattgctat
     5161 ccaagaaaga agttgtgaga attcctccaa tgtacctaga cctccaggga aaacgaggac
     5221 agcatctccc ttttcaaata ataatacctt tctttcatcg atcgtttctg taggaataaa
     5281 tgtagtaaca tcttttccag gcaattcagt ttcaaaaagc ccattcggat agatacctgt
     5341 gacttttccg ttattttcca gtactgtttt actaatcact cccataatcc cggagtatga
     5401 accaccgtaa accagttcga tttcttcttg tgccatgtat tttcctaacg tttgcgcaat
     5461 aaatttgtaa ctttctttat taccaaaacg ggaaccgcaa aaaacagtaa gtttcataaa
     5521 aaaacactcc ctaactcaaa agtctatctt cattctacac gtgttttttt aaaataaaag
     5581 actagatttt gtagtttttt
//
LOCUS       DAJSMI010000162         5590 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJSMI010000162 DAJSMI010000000
VERSION     DAJSMI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.165"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            123..824
                     /locus_tag="ON430_002396"
     CDS             123..824
                     /locus_tag="ON430_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCR3220285.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            821..1942
                     /locus_tag="ON430_002397"
     CDS             821..1942
                     /locus_tag="ON430_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR3220286.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1957..3189
                     /gene="pepT"
                     /locus_tag="ON430_002398"
     CDS             1957..3189
                     /gene="pepT"
                     /locus_tag="ON430_002398"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR3220287.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3321..4229
                     /locus_tag="ON430_002399"
     CDS             3321..4229
                     /locus_tag="ON430_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCR3220288.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4264..4536
                     /locus_tag="ON430_002400"
     CDS             4264..4536
                     /locus_tag="ON430_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCR3220289.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4547..>5590
                     /locus_tag="ON430_002401"
     CDS             4547..>5590
                     /locus_tag="ON430_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCR3220290.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1818 a          964 c         1220 g         1588 t
ORIGIN      
        1 taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata tgggaatttt
       61 tttattccta gtctccttat tttatttgga aaatgctata attatctaga aggagtgaag
      121 ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt gtccctgaat
      181 ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca ttgatattaa
      241 gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat gaatcagcac
      301 gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt gcaaatggtc
      361 tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt atgggaggtt
      421 cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc ggacaagaac
      481 gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg caagaaaatc
      541 agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat gaaatcattg
      601 tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg ttcgggccat
      661 ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag cttcaacaaa
      721 gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt caaaaacaaa
      781 tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca gcggacgcac
      841 tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag gagacccagt
      901 cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga cattagacgt
      961 tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga ttgctaaaca
     1021 tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag aaaaaatgta
     1081 tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca tggatattat
     1141 ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg agagttattt
     1201 agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg tggatcatgc
     1261 acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg attataccgg
     1321 tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg cacaaccagc
     1381 gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg ttattttgcc
     1441 ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt atgaggaacc
     1501 tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag gacgagtagg
     1561 tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag aagcgttcca
     1621 actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga agcgcattgc
     1681 gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa gagcagatgt
     1741 ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg ctgggttgat
     1801 tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta tcgagaaatt
     1861 tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg aaacaaatac
     1921 aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct tttgccacgt
     1981 tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac gactccatcg
     2041 actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga gttaggctta
     2101 gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc aagcaatgtt
     2161 gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga ttttaatgca
     2221 gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat cgtactagat
     2281 gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa ttatgaaggc
     2341 cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa atcagggatt
     2401 gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc tcacggtacc
     2461 atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa attcgatgtt
     2521 gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg tgagttgcag
     2581 tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa tgtacatcca
     2641 gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt tcataatcag
     2701 cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt ccatttgatg
     2761 gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga tcatgatcgt
     2821 gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt gaatcagcga
     2881 tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat gaaagaaatc
     2941 attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga gcttggtatc
     3001 tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc ttacatgggt
     3061 attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt cgaatttgtt
     3121 tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga attgaacaca
     3181 aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat gatggaatgt
     3241 catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct cttttttgaa
     3301 gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa agttcaggga
     3361 aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa aagcagccag
     3421 aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa ttagtcaaga
     3481 aagcagaagc catagaagct gatactttag taattatagg tggagacggc acgattcatc
     3541 atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta cttccaggtg
     3601 gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa gaagctgctg
     3661 acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat gatgaagtca
     3721 ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc agtcaagaag
     3781 aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta ttgctgaaga
     3841 agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga aaatcccaac
     3901 tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac gactcagcga
     3961 ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt ttccgattta
     4021 ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc attacttatt
     4081 tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact cgaacggatg
     4141 gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga ttaacagttt
     4201 attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt tggaagaaga
     4261 gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt gaatttttac
     4321 ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg gcgtgttcct
     4381 gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt gatctctcga
     4441 aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc tgcaggtgta
     4501 cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt cgtctaaagg
     4561 aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg ccattattta
     4621 ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta ccagtgttgt
     4681 tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag ggcgctcaat
     4741 caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt tcttgacctc
     4801 tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga tctcggagat
     4861 ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga cagcactcca
     4921 tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg tcatccaatt
     4981 ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg gtatcctttt
     5041 gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg ttcaaatatt
     5101 ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat gggtggatac
     5161 atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc aagacaggac
     5221 tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt atgtacttcc
     5281 ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca tttcgtttat
     5341 cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg gttcctttga
     5401 atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag tttcttcgct
     5461 tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg tgtatgtgat
     5521 tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag agaataaaga
     5581 aaatcaaggg
//
LOCUS       DAJSMI010000163         5506 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJSMI010000163 DAJSMI010000000
VERSION     DAJSMI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.166"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            41..973
                     /locus_tag="ON430_002402"
     CDS             41..973
                     /locus_tag="ON430_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220291.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1194..2957
                     /locus_tag="ON430_002403"
     CDS             1194..2957
                     /locus_tag="ON430_002403"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR3220292.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3041..3499
                     /locus_tag="ON430_002404"
     CDS             3041..3499
                     /locus_tag="ON430_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR3220293.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3608..4291
                     /locus_tag="ON430_002405"
     CDS             3608..4291
                     /locus_tag="ON430_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR3220294.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RSNTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4438..5052
                     /locus_tag="ON430_002406"
     CDS             4438..5052
                     /locus_tag="ON430_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR3220295.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5068..5274
                     /locus_tag="ON430_002407"
     CDS             5068..5274
                     /locus_tag="ON430_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220296.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1978 a          862 c         1110 g         1556 t
ORIGIN      
        1 tgaaaataaa aatccgtatg tagaagaagc ttgtaacgag ttgattgatt atgtagagac
       61 tcattttttt aaaagaagat attcggaaga tgaaaagtcc tttatcatac gaggaataga
      121 gatcgcaatc ggaagaaagg catttcccct tggactgaaa gaagaagata agaaaagcgc
      181 agaaacgaag ccgttatttc aattagtctg tcaaggattg gaagagcaaa aaggaattct
      241 agacttacaa gaagaagatg tctattatat tttttctttg ttcaacagta gaaattatac
      301 caatgagaac atggagctgt taagaaaaga tttcgaagtt gtttataaaa attttgttct
      361 caatgaccta tcactgaatg aattgatttt acagattatt agtatcgctg aaatcgacga
      421 tgcagaaaat tttattttca agcaatcctt tttacaattt gtccgtacgt tgtgggcaga
      481 tggacaagta tttttaccgg aaaggatcta tcttctatca gaaaaagaac aacttctgta
      541 cggaaaaatt gtagaaatat tggaagagtg gaaaaagaaa aatcaaatcc aactaagatg
      601 gaatgaaaat ttcattcgaa aattcgtcaa aagtctttct ttgatcaaca gtgaacaagc
      661 ggggagacag gaaatagaaa tttttgttgt ttcagaaagt tcagtcaaac aattttttta
      721 tcgaacccaa tttcatttgt atgcggaaga aggcatcgct gaaataaatc ctattttatt
      781 tcgttcatta gaagaattgc ctgatgaatg tctatgtgcg aaaaaaagag tggttttgtg
      841 cgatgctgct agctatcaaa atggattaga aactgaaaag acaaagatct atccaatctc
      901 attaaaaggt gcaaacatcc atttgtatca tattgctcag gaacttcacc tattaaaaaa
      961 acatatttct tgatgagacc ttaattagga gaccttctac aaagcttttt aattgtctgt
     1021 agaaggtttt tatttgagtc ctgattcgtg cgaagatcta cttatataac aagctttctc
     1081 tgaaattttt ttaaaatgct attgacttag agtcaactct aagtagtaga atagataaag
     1141 tgtaacaaat ctattttcat taaaagaaga tagtacggag gagagaatgt attatgagta
     1201 cagtattaca tgtattggat gcaatcaata gccccgcaga tgtcaaaaaa ttatctgttg
     1261 aggaaatgaa acagttggca gaagagatgc gccaaaaaat cttgcaaaag gacagcgatg
     1321 taggtgggca cgttggtccg aatctaggtg tagtagaatt aacgattgcg tttcattatg
     1381 tatttgattc accaaaggat aaaatcgttt gggatgtttc ccatcagtct tatggtcata
     1441 agcttttaac tggtcgaaaa aatggttttg aaaaaggaca ttaccgtgaa gtgagcggat
     1501 attccaacca acatgaaagt aaacatgact attttacagt ggggcacaca tctacatcta
     1561 tcagcttagc aacaggttta gctaaagcga gagatctgaa gaaagaatca ggaaatgtgg
     1621 tagcatttat cggtgacggc tccctaagtg gtggcttagc ttttgaagga ttgaacaatg
     1681 gtggtgtttt acaatccaat ctaatcgtat tagtaaacga taatgagatg tctattgatg
     1741 agaatcatgg cagtttatat aaaaacttag ctgagcttcg tcaaaccaac ggtaaaagca
     1801 gcaataacct atttaaagca atgggatttg attatcgtta cgtggataat ggaaacgatc
     1861 tggaaacggt gatccgttta tttgaagaag taaaagatat tgatcatccg attgtacttc
     1921 acgtccatac ggaaaaaggt cgcggttatc agccggcaat cgataataaa atgaaatatc
     1981 attggcatgt accatttgat ctgtccaccg gggaggcaaa gcaagtaact tctggagaaa
     2041 catttaatat gatcatcatg gattatttgg ataagaaaat gcaaacaagt acagctccta
     2101 ttgccgcagt caatgcagcg ataccaggcg cctttggact gaaaacattc caagaaaaac
     2161 atccagatca ttattttgac gtggggatcg ctgaacagca ctctatcact ttcgcttctg
     2221 ctttggcttc tcagggagta cgtccagtcg tcttccaatc aagtactttc ttgcaacggg
     2281 catatgatca attatcacat gatttagcga tcaacgagaa tccagtgatt attttagtcg
     2341 gtggcggaac gatctcagct agtgaccgaa cacaccaagg aacatttgac attccttact
     2401 tatcaagtat cccgaacatc aaatatcttg ctccagcaac aaaagaggat atgatctcta
     2461 tgctagattg ggcgttcgaa caaacagcgc agcctgtagc aatccgaatt cctacacatg
     2521 gtgtagaaca tggtacagct atcgcaaatg attatagtat tccagcctat caaacagtaa
     2581 ataatggagg aaaagtagca attttagctt taggcggttt tcttgaatta ggaaaagaaa
     2641 cagtaaaagc ttttagaaaa aaaggaatcg aagcaacgtt gatcaatcca ggaacgatca
     2701 cagaaaatga cgtagaaaca ttagataaat taaaagaaac acacgaatta gtcatcacat
     2761 tagaagatgg tagtttgtca ggaggatttg gcgaaaatat cagtcgttat tatggtgatt
     2821 cttcaatgaa cgtattaaat tttggtgcag agaaagaatt cacggatagc gttcctgtag
     2881 ggcagcttta tgaacgttat catttaacac caaaacaaat cgttgaagat agtctgcgtg
     2941 tcttgaatat tcaataattc attatgctca ccatccagat aattgtttat actagtatct
     3001 ggatggtttt ttcattataa aaagcaaagg ggtggatgtt atgtatcagc aagtagaaga
     3061 atatgtcaaa aagttccaag aaaaatatcc tagttatcaa tcagaaaaat taggtttctt
     3121 ttcatttggg gatacagaaa aaatggcaga cgagttgagc gaactagttg taaaaaagat
     3181 aaagactggt acatcaagtg gttacgatct gtataaacaa gagaatgagc ctttaccaaa
     3241 agttggtcaa ttagacgtgg tcctgaatgg aaaaaatgaa ccagtttgta tcatcaaaaa
     3301 tataaatgtg accatcttgc cttttagtga agtcacgaaa gagcatgctt ggaaagaagg
     3361 agaaggggat cggacattag cttattggcg gaaagtccat gctgagtttt ttaaacatgc
     3421 ttataaagaa gcgttgaaca tagaatttac agaagagaag ctggtcgtat acgaagaatt
     3481 tgaagtaatt ttcttttagt aagaaaggtc tgctgaaaga aatagctcat tattgactcc
     3541 tgaatagaaa agggttacat ttttatatga agctgtattt ttatataaca ttactggagg
     3601 agatattgtg gatacactgg aacaaatcaa aggcggagtc gtcgtttctt gtcaagcttt
     3661 ggaaaacgag ccgctacaca gttcttttat tatggggaga atggctttgg ctgcaaaaga
     3721 aggcggagca gttggtattc gatcaaatac gacagaagat atcaatgaaa tcaagcgaat
     3781 cgttgattta ccggtaatag ggattatcaa aagagattac gatgggtcag aagtgtttat
     3841 tactgcgaca gaaaaagaag tggaagaact tttggagacg caagcagaaa tcattgcact
     3901 ggatgcaact tctcgtaaac gtccgacaga aaaaacaact gctgaacttg tccaaatgat
     3961 tcacgaacac ggtcggttag ctatggcaga tatttcgact tttgaggaag cgcttcaagc
     4021 gcaagaagat ggttttgatc ttttgtctac gactttagca ggctatacag aatattcacg
     4081 aaagacgcag acacctgatt ttgatttatt gaatcaaatc atagaacaag tcgatatccc
     4141 agtaattatg gaaggacata ctgactctcc tgaacaagta gagaaagcat atgaattagg
     4201 tgctttttca gtcgtagttg gatctattat cacccgtccg caattgatca ctaaaagata
     4261 tgtcgaagcc gcaagtaaag caaataacta aaaaacacgc acggcacaga ttcttgacaa
     4321 aaaagaaaaa ttgatagata gtttaaagca gattctaaga agatgaaaaa agcattactt
     4381 ttcattttgt cttcaagatc tgctttttaa cgaattttat agaaagaagg cgaaaccatg
     4441 tttgaacgga gtccttcaga attgacagaa cgtgaaaatt acaagttgct gattggttca
     4501 gtcatcccta gaccagtagc agtagtaacg actaaatcag caaaagatat agtgaatatc
     4561 gcacccttca gttacttcaa tatcgtcagt tcgaatcgac ccatcttatc cttggcgatc
     4621 cagagaaaac aagatgcagt aaaagacaca gcaaagaata ttcttgagac aagagaagct
     4681 gttatccata tccttgatga ggacaacgta gaagaagtaa ataaaacagc agccaatctg
     4741 cctaatgagg aaagcgaact gtacctgact gacttgactt taactgattc agtaatcatt
     4801 tcagtaccag cattgacaga agcaaaagtt cgttttgaaa catccttata tgaacatgtc
     4861 gagatacgga ataaaaataa agtaacagct gatttatttt tgctagaagt acagaaatat
     4921 catttaaatg aaacagtcta tgatgataag acaggaagga tcgatccaag aaaattgaat
     4981 gctgtaagcc gtttggcagg aaatgattat gcagcaatag gagaaatttt cacgattgca
     5041 cgtcccgaat aaaaaggagc actagttatg ttaaaaataa gctataaacc atcgacagat
     5101 tcaaaagaaa tgaaaaaaga atatgaaaca gtgaatgatt ttctgcaagg gcagtactta
     5161 gaagttccgc cattgcaaga ccattttgtc gttactactg ttactttaga tggtaaggaa
     5221 atagaaatgc cagatcaaac gattagcgga ttgtttaatt actttaacaa ataaatattt
     5281 attccgaaaa gtgaaaaaaa catataaaaa aacacataaa actaaaaaat tatcaaagaa
     5341 tcctggtgca actgaaagaa tttgcaacgg gattctttca acttatttgt tatttttttc
     5401 taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca
     5461 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataac
//
LOCUS       DAJSMI010000164         5407 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJSMI010000164 DAJSMI010000000
VERSION     DAJSMI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.167"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            82..2409
                     /locus_tag="ON430_002408"
     CDS             82..2409
                     /locus_tag="ON430_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3220297.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2535..2864
                     /locus_tag="ON430_002409"
     CDS             2535..2864
                     /locus_tag="ON430_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR3220298.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2904..3908
                     /locus_tag="ON430_002410"
     CDS             2904..3908
                     /locus_tag="ON430_002410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3220299.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3905..5334
                     /locus_tag="ON430_002411"
                     /pseudo
     CDS             3905..5334
                     /locus_tag="ON430_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         1924 a         1031 c         1067 g         1385 t
ORIGIN      
        1 ctatacatca ataaaataat aaaaattaaa tttagaataa cgtgtaaagg attatttgat
       61 gattttttat tgagaggagg gatgaaaatg attaagaaaa ctcaaaataa aatccaacgt
      121 atatgtcaaa aacattttgt ttctagactc aaagctgtat ctaaaagaaa atacttacat
      181 cccattatga tttcgatcat cttgattttt actttcttag ggagcttatc aagtatttat
      241 gtaaacgttt atacagaaac gtcaaatttt gctaaccaaa cgacagagga gacaaatagt
      301 ccaccctatt ctgaaataca gtccatagaa agcaccaatc agtctagcaa caacgagaca
      361 acctcaacca catcctcgac tcaactgatt gatccgacag aaaaagaaat cacagcagca
      421 aaagcagcag gggagaaagc ttattcagaa acaggtagaa gccagacatt tagtactgtt
      481 gcggccactg cgaatttcaa cggacatacc ataccagatg gatatacagt gatatatgcc
      541 aaagatggag tcattgttgc ggttaacgga tcgatgagtg cggataaaac tgcgttacaa
      601 ggcgtaacca aagcaatcgc ttttattgat aaccgtccag atatccataa tctgtttatt
      661 ggctccacag taaatggtac tactatcaca ttcccaaaaa gtatctatat gggggtggct
      721 accgctggaa atacgatcga ttcagttaca cccaatgcgg ataaaagtta ttcttttaca
      781 ccctcaacat ggggagggct tcctgccgga ggaggtatga gtactttttc aatcggtata
      841 cagttagcaa atgatattcc attcacgggg tactcgatca aaaatgggca gatcgttgat
      901 ggtaagggaa atgtttcaaa ttttccaacc tatcaagcag caacggttca ggtcaatgcg
      961 aatcggacag gaactttttc ttatacagtc aatggtggag cagcaaaaat cggaacgtac
     1021 gggactacat tatctggaat ccatgtgggt gacaaagtgg ttatcactcc aacagcagta
     1081 acgggctatt cttttgcaca aacaaacggt tctttcacag ccctagaaag taacgtcgat
     1141 acggtgacct atacaggcaa acaaacacca atcactatta agtatcaaga ctattttgga
     1201 cagatgattg ctccttctaa aataatgaat cttacttacg gaagtgctgc acaagacctg
     1261 acgactaata cgcctactat caacggatat acctttacag ctgtttcagc gacagaaaca
     1321 aagaaccaat cagcaatgag tgttttagct agtttagata caaatggaaa tgtaatcgta
     1381 aaggacgcaa atggaaaaca gatttctgaa gtcatcctat attataagac aaatgcaaca
     1441 gtatcaatca atgcaaatgg cagtaagtac tatgatggtc tttctgttgt accgatcgtt
     1501 aaatatgcct ttaatgataa aacagaatca acatcccttt taaacaatga tctcagtatt
     1561 gatcaaatca catggaatcc aactgatttc aaagcgatga atgaaaaggg agttgaagta
     1621 actgcgccta ctgaaattgg aacctatacg tcttggcaat tgacaaaaag cggactagct
     1681 aaactagctg ccagaacaaa ctatttattt actatcgtgc agacatctga tgtctatacg
     1741 attaagcaaa tcagcggtgc ggtagagctc agtggcagta agacatatga tggaaaagca
     1801 ggtgtcccgt caatccatgt caagctagca gagggtgtca ctagttcgct tactccagta
     1861 caagtggcac ttagcagtat ggattacaca gcagatgcac aatcagcaaa aaatatggtt
     1921 aatgctggca gctatacaat taaactaacg aatagcggaa tagacaaagt caaagcagca
     1981 aatcccaagt tgtcatttac tgatatagcc aacacatcag gaacttatac catctataaa
     2041 gctaacgcag tgattactgt cgatgacgct gcctttaatt atgatgccca aagtcactcg
     2101 atttcggttg gaaatctcca cgttaccggt gttgtcttag gcgaatcatt ggattatacc
     2161 ctaacagtta acagccggac agatattgga tcacaaatag taacgattag tttaggcgaa
     2221 ggaagtgtaa acaataatta tacgattatc gcaacagata ccgctaagtt gacgatcaat
     2281 ccgactctct tgcttccttc gacaggtggg ataggtctac tgccctttgt ctttttagga
     2341 ctgatcttta ttttttctgg cttcttttat tttatccatc gtaagaaaaa agcaggtgaa
     2401 cagcgatgaa aaaaacaaaa aatgtacgtg tggatatatt tgctgttctc ttttttatcc
     2461 tactggtctt ttttataggt tctccgactg tgtcagctag agaatcacct attacattta
     2521 ctttgcataa tcgaatgttc gaaagcgata agatgccaag tatcagtcaa aacgacggaa
     2581 taaatatccc ggataacttt caaggagtaa aagggttgaa cggcgttact ttcgctatat
     2641 atgatgtctc ggatgaattt tacaagctgc ggtcagaagg aagtagtgtg gaagacgcac
     2701 aaagaaaact agcacaaaaa agtgatagtg aaaaaatact ggcagaaaat gtgacaaaaa
     2761 cagtagacga ggaagagggc attgcttctt ttagtgcatc ggacaaagat gagcaaggtc
     2821 gtgacgctgt ttaccgattt gcggaaataa agacttcaga ttaagtaaaa gaaaaatcag
     2881 caccatttgt cgttgtgctt cctgtgacag attcatctaa tcataaattg acgaccatcc
     2941 acttgtatcc aaagagcgaa caaaaaatac ctgctgcact cacattaaca aaaacagtag
     3001 agaataagca gacagatttt gcagatgggg ataaagttcc ttatctcatc accgtaacaa
     3061 ttcctgagaa catcaacgag ataggaacct atacgatcaa agatacagct gatcctcagt
     3121 tatggctaga accagaaacg atcgattttt tgattggtgg agacaagatt catacatttc
     3181 acacacaaaa aacaaaacat ggttttgtct tatcagattc tggaaaagat cttagttcgt
     3241 ttgctgggaa aaaattaacg atccgttacc aaatgacatt gaaagaaaac agtgaaaaaa
     3301 cgacatttga taacgatgta cgtttgacga ctgacaatca aacagtagaa acacatcttg
     3361 ccatccaaac aggaggcaaa caatttgtca aagtggatcg gcaaacgaaa caaaaactag
     3421 cagatgctca atttctagtc aaaaaaaatg accaatactt agcgcaagaa aacgggataa
     3481 atcattgggt agccaaagat gattcaagag caacgatttt tacttccagt aaagatggaa
     3541 cattttctgt ccatggattg tcttttggaa gttacgaact agtggagatt aaaccgccta
     3601 aaggatatat attgagtcaa tcaaccattc tctttgaagt tgaaaaaggc agttatgatc
     3661 cgcttcatcc tataccattg gaaatcatca acgaaccaaa agcaaatccg acgaataatg
     3721 gaaaaacgcc agttcagatg cagacaaatg gtccatcaaa tgaaaaaaat ggaggaagct
     3781 ttccaaaaac aagcgaagag atgcttggag gtttctcaat tttagggttg ttgctagtcc
     3841 tttcaacagg gacagcttgg ttttataaaa aacaacaaaa aggaaacaat aggagggaaa
     3901 taaaatgaaa aacatcaaaa caaaaatatg gacgttttta ggaactgcta tcatgctgct
     3961 tcctttcgtt ctcggcctag gaactgcaga ggtttctagg ccgtatcacc tacaccagaa
     4021 aatgttacgg ttaatttgca taaattgaaa ttcacttcag caccagaaaa tcaaataaac
     4081 aacggaacgg aattgacatt tccgaatagc gaaccgttga acggcgttga attcaacgta
     4141 tatgacatca ctgctacgta ctatccaagt aaagacacag cagttccagc agacgctaca
     4201 cccttcgcaa gtgtaacgac ttctggtgaa ggattagcta acctgactct tccaggtaaa
     4261 tctgatggga aagatgctgt atacgtcttt gtagaaacac caaagcccgg tgtcgaaacg
     4321 tcgccaaata tcgtgttaag cttacccttt tataacccga attcatcaga ccctgacaaa
     4381 cctttagaca cgattcacct atatgctaaa aatgtcatca aagaaagcaa tatcaaaatc
     4441 cagaaagctg ggaacttttc aggggtagat aagttagcag gggcgcaatt ccagatcaaa
     4501 aattcagaga atcaattcgt gaccggtttt gatgaaaaca ctggtgtcgc tctctatgat
     4561 cctgctgagt cagcgttgac attcacaact gatactgaag gatccatcag tgtcaatgga
     4621 ttaatagacg gcaattatac tttagttgaa acaaaagcac cagatggtta tcaattaacc
     4681 aatccacaaa aagaaactac ttttatagta aaaaacggaa aattgcaaca aacaaccggt
     4741 atcatccatg gcgcagatga agccaatctt gggaatccaa acggtgtagt caatcgggaa
     4801 aatacaacta cggataacac gattactgta gaaaatgtcc aaaactacgg tgatttccac
     4861 ttcatcaaac aagatgtgaa cacgcaagaa aaattagccg atgcagaatt ttctattcta
     4921 agagcaaaag atccaactgt taaattaatg aaaaaaatag caaatcttgc ctcaggagaa
     4981 tacccatatg cttggtcaga tgaagtcaca ggttctgact gggaagaagt gaaattgatt
     5041 tctgattcag aagggaaatt ttcaatcgaa gggctgaaac aaggggcata ctgccttcaa
     5101 gaaacaaaag caccagaagg ctacgcatta cctaagaatg aagctgcgta cactgaattt
     5161 acagtagatg atgatccaac aacttctgat gaaacaacga tcgataacca acctaaagga
     5221 atcttgcctt caactggtgg tatggggatc atcgtattca tcttggtagg tactgcttta
     5281 gttggaggag cagtgatcta cttcaaaaaa cgccatcaag aaacagaagc ttaaactatt
     5341 ggaaacaaaa atcatacgat aaataaacga tagtgattca aatggggaga aaggttgtga
     5401 caagtgg
//
LOCUS       DAJSMI010000165         5268 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJSMI010000165 DAJSMI010000000
VERSION     DAJSMI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.168"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            1..200
                     /locus_tag="ON430_002412"
                     /pseudo
     CDS             1..200
                     /locus_tag="ON430_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            316..909
                     /locus_tag="ON430_002413"
     CDS             316..909
                     /locus_tag="ON430_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3220300.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKEAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDEYAIQPHYTNKENFDLGITETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            925..1824
                     /locus_tag="ON430_002414"
     CDS             925..1824
                     /locus_tag="ON430_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR3220301.1"
                     /translation="MTPTQFKAKTRNFATEKHLNPQIVQRYYMMEKLLERIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTADPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERINEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKVERKQNFKRSQQMNQEMER"
     gene            1827..2312
                     /gene="ssb"
                     /locus_tag="ON430_002415"
     CDS             1827..2312
                     /gene="ssb"
                     /locus_tag="ON430_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3220302.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTATFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2357..2590
                     /locus_tag="ON430_002416"
     CDS             2357..2590
                     /locus_tag="ON430_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220303.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGRDNSFWRLTKVTIAATAVILTAILPLYM"
     gene            complement(2749..2871)
                     /locus_tag="ON430_002417"
     CDS             complement(2749..2871)
                     /locus_tag="ON430_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCR3220304.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3026..3286
                     /locus_tag="ON430_002418"
     CDS             3026..3286
                     /locus_tag="ON430_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220305.1"
                     /translation="MDKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3729..3935
                     /locus_tag="ON430_002419"
     CDS             3729..3935
                     /locus_tag="ON430_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR3220306.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     QTCGKKYTLSYGDYVLIDEKGKKH"
     gene            3935..4186
                     /locus_tag="ON430_002420"
     CDS             3935..4186
                     /locus_tag="ON430_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220307.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            4202..4639
                     /locus_tag="ON430_002421"
     CDS             4202..4639
                     /locus_tag="ON430_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220308.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            5084..5263
                     /locus_tag="ON430_002422"
     CDS             5084..5263
                     /locus_tag="ON430_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220309.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQKLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
BASE COUNT         1896 a          884 c         1052 g         1436 t
ORIGIN      
        1 caggaagctt ctccttaggc agagttcaga ctccaacgct ttatatgatt tatcagttgc
       61 aagaaaagat taggaacttc aagaaagaac cctactttga aggaaaagcc caagtaaccg
      121 ctcaaaatgg cacatttgat gccaagcttg atcccaatga aacccaagtg acgcaagaag
      181 catttgaagc gtatttaaag aaccagcagc ggattcaaaa agaaattgaa ttagatcgat
      241 aaatttcttt cttgttaata taaaactaac atgttatagt tatgttagtt ttatattaac
      301 tttaggggaa aagcaatgaa agacaaaatc cagtgtattt ttgataaaca taatgggaat
      361 ctatccttga aggatgctag aagtgaagga ttatcgccta tgacgttaga tcgattagcc
      421 gaaaaggaca aaatagaaaa ggtagcacca ggtttctata ccttaaaaga agcgttcgtg
      481 gatgaattgt atctcgctca aagtatctat tccagaggga ttttttctca tgagactgcg
      541 ctcgatcttt ttcaagtagg aacgtatatc cctaaaaaga tcaatatgat gtttcctaaa
      601 ggatacaatg tttcaaagga gaaattagac gagtatgcta ttcaaccgca ttacacgaat
      661 aaagaaaatt ttgacctggg aatcacagaa acagaaagct tttacggcaa taaaatttta
      721 gtctatgatt tagagcgaac gttatgtgac atgtggaatc caagatacaa agcaagcgta
      781 gaggtaaaac aagaagcatt aaaagaatat atgacaagta ccagtagaaa tacaaataag
      841 ctaagaagat acatggatat tttaaaatca ccaaaagaaa tgactttgta tatgttgcca
      901 ctttattgag aggagtaaag gtgaatgaca ccgacgcaat ttaaagcaaa aacgagaaat
      961 tttgcaacag agaaacattt aaacccacaa attgtccaaa gatattatat gatggaaaaa
     1021 ttattagaac gtattgttga atcgccttat catgatgatt ttgtattaaa agggggattt
     1081 ctgatcggat caaaatacgg gttagaaaac cgttcaactg ttgatattga tacgacttat
     1141 cgaaattcga aactaacaaa agaaaagtta gaaactgttt tttctgaatt aacagctgat
     1201 cccacaaaag atggaattcg atttgaatta aagggattaa cagagacgag ggaagctgat
     1261 tattacccag gttttcaagc aaagtttatt gcttatcttg agaatgcacg tgtaccattt
     1321 aaattagata ttacttcagg tgattctatt attccagcgg cattgatgta tgaacacaaa
     1381 ttaatgtttg aagataaaac gattcatatt ccagcttatc ctacggaaca aattctagct
     1441 gaaaaattac atgccacgtt tagttttgga aaagataatt cgcgcatgaa agatttttat
     1501 gacctgtata tgattccgaa attagaacgt atcaatgaga aaaatttgta taaaagtgtt
     1561 caaggaacat tcaaggaaag aaataatcaa tcggtgttta agcatttgta tgaccaggaa
     1621 atgcctcaat tggtgaagga tgaggggttg agggaaaagt ggaaaagata ccaacaagaa
     1681 aactattttg ccaaagacat ttctttcgaa tctacactaa cttccgttga ttccctgatg
     1741 caatcaattg tagagcaaga gaaggtagaa cgaaaacaaa attttaagag atcacaacaa
     1801 atgaaccaag aaatggagag ataaagatga ttaataatgt aactttagta ggacggttaa
     1861 ccaaagatcc agatttacgt tataccgcaa gtggcacagc agtagcgact tttaccctag
     1921 ccgtcaaccg aaatttcgcg aatcaaaacg gcaatcgaga agccgatttt attaactgcg
     1981 tgatttggcg caaaccagca gaaacaatgg cgacattagc taaaaaaggc ggcttgatcg
     2041 gtgttgtcgg acggattcaa acacgaactt atgacaacca gcaaggacaa cgtgtgtatg
     2101 tgactgaagt tgtagcagat aactttcaac tattagaaag taaagcagcg acagagagcc
     2161 gtgcgcacgc tgatcaatcg tccaccagtc caagcaccgc cacctttgaa caacgggata
     2221 cagcgacgcc taacaacaat ggattgaacg cttcacagaa tccatttggt ggacaatcaa
     2281 tcgatattag cgatgatgat ctaccgtttt agttcgagaa agaaggagta agcatggcaa
     2341 aaaaaatagt ctgacgatgg atctattgat acccgtaatt gcatttatag acgggatttc
     2401 tttgggcgtc actgaaaatc ctttggtaaa tagaattgcg aaaggatcac tatttcttgt
     2461 attcgtgtat cttgtatgtt cttatctttt aaaaagagca ggaagggata attccttttg
     2521 gcgattgact aaagtcacaa tagcagcgac agctgttata cttacggcta ttctaccgct
     2581 ttatatgtag ggttaacggg aaatcgttga acttcttgcg aaatattgct ataatgaaca
     2641 tgaaaaagag gagttgcgct aacaacccct cattaacagc accgattaaa gaacggtggc
     2701 agaatattag ttatttggaa ataaccgctg acctgctaaa gtggacggtt atttcttttt
     2761 gtcattttta agcatattta caatcaaact aatcaaagca atggtaaaca taccaaaacc
     2821 aagaatggtt tgaattgtct catatgcgga caaaaggctt actcctttca tagattttag
     2881 cacttacatt cataagcacc accacctttc cagaagtcgc caccgtcttc aaactttact
     2941 gtcttgacta ttatagcata tctaaaatcg aataaaaatt ctcaattttg gttgtttttg
     3001 ctataatttt agcgaggtga ttgtgatgga caaatatgtt ggtttgcttg ataaaatccg
     3061 agtaataaaa acacaaccat tattggttcg ctttacttta caaacaattc atgaatcgat
     3121 taattgtgta gttgcagata ttgaaattat cgataaactg ctaattatgg atgatggaaa
     3181 atacaatatt gccgtcacag gtcacttcaa taaacgaaat cagctagtta ttgctagcat
     3241 gtatgttaga aatcccgatc attttacgcg atcgatgggc atataaaaga agagttgtag
     3301 tcctaaatga ggattgcagc tctttttgtt ttacatagaa acgggaacag gaaaaggcct
     3361 gtttaggaag gcacaagcgc cttctcaatt gaaaaacctt tctctcactc caaccgccac
     3421 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taaaataagg ggtcttgcaa
     3481 agcaagacaa aataggaaaa acttcctctc accaaaaggt tcgttcctgt tcgctatctc
     3541 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     3601 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     3661 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     3721 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     3781 actatcatgc accacatact gtcgaatgcg attgtggaga aagagcgaaa tgtatcaaaa
     3841 gctatcgttt gtatcaatgt caaacatgcg ggaaaaaata taccttgtct tatggcgatt
     3901 atgttttgat tgatgagaaa ggaaagaaac actgatgaaa acacaacaca aattattgac
     3961 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     4021 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     4081 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     4141 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     4201 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     4261 aatggaggat atgacgttcc tatacaacga aacacgtgtt ccaaagaaac attataaaga
     4321 aaaattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
     4381 gacgtactat cgaacactgg aagaactgaa aaagcaaaat gcaaaatggt ttttccaagc
     4441 cttactatgc ttggaagtag gcgtcaaacc aagcacaatc aaaccttctg aataccaagc
     4501 tttagaacta acgtatgcga aatttatcga aacgaaaaaa gccaagactg tttcaagtga
     4561 atggttagat tattttgaaa atatcaataa gtatggcgca tattacacca tgaaaaagga
     4621 agacaacgaa aatgaataag gtgatttatc aactaacaac ggattccata tcaacgattt
     4681 gaaagaaaaa aaggtcaata atcatttata ccgaaaagtg agaatgtgtg aaataaggac
     4741 aaaaaaagcc tagtaactaa gggaatggtt cttatttcgc accaatcgct ccaaccgccc
     4801 ccgccgctta ggtgatgcga aataaacggg caaagcccga taactcaaga gaaagaaggt
     4861 gatccatttg aacaagatta ttcccttatt gttagtacta tttgttggcg aaataattat
     4921 gcgacatagt gcagctgtgc atttaaactt cgtagtattt ttggtaatac tggtgttttg
     4981 tttgatcttt tggcgctttg tgttagccct tgtgagtcta atattcaagt tgattgcagc
     5041 catatttttc atagggttat ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg
     5101 aaataaacgg ttacccgtta cgttggatga aaagagacaa aaagaattgc agaaactaaa
     5161 gcagaagtac ggaaaaagtg aatctaagat tatgtgtatt gcgttagatc tattgatcgc
     5221 gcaagaaaaa gcaggatttg atgtaccagc actcaaaaag tgacgtca
//
LOCUS       DAJSMI010000166         4990 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJSMI010000166 DAJSMI010000000
VERSION     DAJSMI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.170"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(54..1202)
                     /gene="nagA"
                     /locus_tag="ON430_002423"
     CDS             complement(54..1202)
                     /gene="nagA"
                     /locus_tag="ON430_002423"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR3220310.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1278..2114)
                     /locus_tag="ON430_002424"
     CDS             complement(1278..2114)
                     /locus_tag="ON430_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3220311.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2298..3515)
                     /locus_tag="ON430_002425"
     CDS             complement(2298..3515)
                     /locus_tag="ON430_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3220312.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3664..3843)
                     /locus_tag="ON430_002426"
     CDS             complement(3664..3843)
                     /locus_tag="ON430_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220313.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3898..3981
                     /locus_tag="ON430_002427"
     tRNA            3898..3981
                     /locus_tag="ON430_002427"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3932..3934,aa:Leu,seq:gag)
     gene            complement(4221..>4990)
                     /locus_tag="ON430_002428"
     CDS             complement(4221..>4990)
                     /locus_tag="ON430_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220314.1"
                     /translation="YNYKLSVFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFN
                     FLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLII
                     YFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLC
                     LIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVL
                     MAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1531 a         1027 c          799 g         1633 t
ORIGIN      
        1 aaaccttaaa accaaactta agaagtttag ttttaaggtt aacttaaatt atattatgcg
       61 tggaaacgtt ccacgccatc tagataagtt gcttccaatg tcatatcagg gttcaatacg
      121 ataaagtctg catcgcgtcc ttgagcgatt tttccacatg tatcatcgat tttacagctg
      181 attgctggaa tcagactagc catcatgact gcttgttctg gtgttgctaa tccccagtct
      241 acaacatttt taattgcttc tttcaatttc aatatacttc cagctaagtt gccagattct
      301 agacgtgctg ttccttcttt tactactact ggaaattcgc ctaaattgta atttccatca
      361 ggcatcccac cggccatcat acagtctgta attaatgcga catgatcatg tcctgctttt
      421 tccattagga ttcccgcagc atttggatgt acatgatggc catcacaaat caattcagaa
      481 aagacatgtt ctaaagataa taacgcaccc accatacctg gttcgcgatg attcaatcca
      541 cgcataccat tgaaagcatg gacaaataca cttgcgcctg cttcaacagc tttttgcgct
      601 tcttctagtg tagcattact gtgtcctaga gaaacaacta ctccttcttc agtcacttgt
      661 ttgacaaact cttctacacc ttgacgttcc ggtgctaaag caattttttt gattaaacca
      721 cctgaagctt cttgccattc atggaaagta tccaaatcag gatccccgaa ataacttggg
      781 ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt caaaatagat tcctttgatt
      841 ttagctcctg gtgcttcctg atacattttg ccgattgtct cagctacatc ttttaaacgt
      901 tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca ctccacaaga taacagacct
      961 tcagacatca ctttgatgcc ttctgcatca ttatccatta catcatgatt catatagccg
     1021 tgaatatgtg tatctaccaa accaggagca atccatttgc cgctttggtc aatgatgttg
     1081 acttctccct caggtagttc tttggtgtat tcaccaaaaa gtccatcggt aatttcaaga
     1141 tagcctgcat gtttgacacc tgacgctaaa aagaatttgt cagcatagat aaaagttctc
     1201 attctattct ctccctttgt ttttctacct ctaaagtact cttatttaga gaagaagtca
     1261 agaatggtct acaccattta caaatttttt gaaatttgtt tcacagcttg tgctaagcct
     1321 tcatagaatg ctgctctttc tggttcttct ttacgcttgt atattaagct tttaagcaca
     1381 aataaatcat tgaggtaata gtagctattc ttctcttgag cccaatagat tccttgattc
     1441 acctgttcta acgcttcgtc gatttcattt tgatggaata agaaatttcc ataattataa
     1501 aaaatttgcg aaaattcact ttttgatcgt tcatcagctg attgatgcag cagttggatg
     1561 cttcgactca aatagtagcg tgcttcagcc gtttttcctg ccacaccata tatttttccg
     1621 atacaactaa tcagttgtat ttctgtgtca gaaacatgga ttctttcttt ttggctagag
     1681 tagcttaatg cttctttcaa ataataaagg gactgagaga tattttgacg caacgtgaat
     1741 tcgcaaacac cgagataata ataatagcgt tgaaagtcag ttgcttgata cagattttca
     1801 gaaatatcag aagattttag tacttgttct aacttccgat aatcacgttt ttggaaacaa
     1861 ctatccaaca gttcaaataa tttatcgttt gtccgagtag tttttcgaga gagattcatc
     1921 acatgatcaa tcgttacccc tagccgttga caaatctgca tcatcaccaa tacattcggc
     1981 agttcctcat tattttcgat ccgacttaat acactttgtg aacaaatatc atgcgaaagc
     2041 attttttgcg tcattttttg ttcttttcga atctctttaa tgatcgttcc aaaggactcg
     2101 attaacgttt ccataagtat cctccccaaa atatgcgtat tcgcattcta ttcgtatttt
     2161 actttaggaa gtcgcaaact tcaagaaaag acttgcaact aaataatata ctttagttat
     2221 ttaagaaaac tccactagaa agagagcctc agtgtgtttg tctgtggccc tcctactgaa
     2281 aaatttggtt tttaaaatca tttttggcga taggttgcta ggaattctct cattttctta
     2341 gctgtttgtt tcaaatcatc cattttagga agataaacga ttcgaaaatg atctggctgg
     2401 ttccaattaa atccaccacc atgtacaagt agaatatgat gttcatgtaa aaagtccaaa
     2461 acgaattttt catcatccaa aatattgaat cgctttgtat caatttttgg gaatatataa
     2521 aaagcagctt ttggttttac ggctgaaaga ccaggaatat cattgattgc attatagata
     2581 tattctcttt gttcatagat ccttccgcct ggcaggagca gctcatcgac actttgataa
     2641 ccgcctagcg cggtttggat aatttgctga gacaatacat tggaacacaa acgcatggag
     2701 gacaacatat tcagcccttc tatatagcct ttgacgtgtg acttatctcc acttaggacc
     2761 atccagccta ctcgaaatcc tgctacacga tgagatttgg atagaccgtt cagcgtaaca
     2821 acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt ctagaccatc catcactaag
     2881 cgatcataga tttcgtcgga ataaataatc aggttatttt gtcgagctac ttcaacgatt
     2941 tcttctaata attcttttgg ataaagtgcc ccagtaggat tattaggatt aatgataaca
     3001 atagcttttg tccgactcgt tatttttgat ttgatatcat caatatcagg ataccattct
     3061 gcctcttcat cgcaaatata atgaacaggt gtgcctccag ctaatgcaat agaagccgtc
     3121 cacaacggat agtccggcat tggaaccagt acttcatctc cattgtccag caacccctgc
     3181 atacacatcg tgatcaattc gctcactccg ttgccggtgt agatatcatt gatcgtcaca
     3241 ttcggaaaat gtttcagttg gcaatattgc tcaattgctt ttctagcaga aaagatcccc
     3301 ttggaatctg agtagccttc tgagtcacgg gcattcatga tcaaatcacg aatgacttcg
     3361 tttggtgctt caaatccaaa aggtgcggga tttcccgtgt tcaattttaa aatactaatg
     3421 ccctcttctt gcatccgctc cgcttcttct agaacgggac cgcgaacatc gtaactgaca
     3481 ccttctaatt tgttggattt ttcaaattct ctcatcactt agccaatccc tcatttcaaa
     3541 aagttagaac tttccaaatt ttttgttatt tactatttca atatcatact ccatcctaga
     3601 aaattttgcc acttatttag gaaaaacgta agtattaaaa aaacacaaac aagcgttgag
     3661 aacttaattg tcatgatagt cattccatag attcgctgcc caatccattg caattccaca
     3721 aatgattcct agcataaaac caagcaaaaa acgtccgaat gcaagtccaa tcaatacacc
     3781 taagatcatt cccagtcctg aaaattctcc gtcatatttc tccatcttaa taagtcctcc
     3841 catttttgag cttgacaact atgtaaaacc tcgctatcat tagtgtgtta ttattacgca
     3901 gatgtgttgg aattggcaga caagcatgat tgagggtcat gtgagcgtta gctcgtgtgg
     3961 gttcgagtcc catcatctgc actacaactc gtgaagttaa ttttgcttca cgagtttttt
     4021 tgtttgaacg tttctttatg ccattatata catatcactg tatcctttac aaaaagaacg
     4081 tacaatcatg taaacagcct gaggtgagta tatgaaggtc ggaaacaagc tactcgtaga
     4141 aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa aatccctcac ctaatactaa
     4201 agcgagggat tttttgtata ttatctgttt atcaccgtta ttataaaggt acaaatacca
     4261 agcacggtta atccaaattg cataaattca tttttttgta cactcatgct tttcattagc
     4321 tctttaactg ccattagtac taagatggcg ataacaacat aaagtatata gtacgtccgc
     4381 attccggaag cccatactgt gggagaaaat cccataatta accttgaaca aaatcccatt
     4441 atcatcaaaa taactaaaaa aatagaagtt ttcttattgt caaaacttaa ataaattcca
     4501 acaattaaac atagtaataa tgccaagact actaaagtag ctatccaagt acctggataa
     4561 aaaagtgaca gtttcgttcc taactctgta aagagattat tcaggttact actactccaa
     4621 atcattgccc ttttatttcc attgacgtaa gtaaagcttt gacccattgt attaccaaaa
     4681 taaataatca ggtttaagaa aaacggtaat gcagtcaata tacgcacata atagttttga
     4741 catttcctat atgttagaag gaaaattaag aaaaatagca ataaaaataa gatattcatg
     4801 tctaaaaaaa acggctttcc aaatgatgaa atacctaaat ctaacttatt aagaaagttg
     4861 aaatttttat attcaggaaa ccatttatta atttcatgtg cagcacgtaa tgcattccct
     4921 ggagttgtca aagagaaaat taattctgct aagcttatta ttgaaaatac tgataactta
     4981 taattatatc
//
LOCUS       DAJSMI010000167         4972 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJSMI010000167 DAJSMI010000000
VERSION     DAJSMI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.171"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..134
                     /locus_tag="ON430_002429"
     CDS             <1..134
                     /locus_tag="ON430_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3220315.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(220..1314)
                     /locus_tag="ON430_002430"
     CDS             complement(220..1314)
                     /locus_tag="ON430_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3220316.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1503..2264
                     /locus_tag="ON430_002431"
     CDS             1503..2264
                     /locus_tag="ON430_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3220317.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="ON430_002432"
     CDS             complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="ON430_002432"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR3220318.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3039..3794
                     /gene="fabI"
                     /locus_tag="ON430_002433"
     CDS             3039..3794
                     /gene="fabI"
                     /locus_tag="ON430_002433"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR3220319.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3904..4917)
                     /locus_tag="ON430_002434"
     CDS             complement(3904..4917)
                     /locus_tag="ON430_002434"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCR3220320.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
BASE COUNT         1545 a         1003 c          863 g         1561 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc ataacctata aaaaaacaaa ataaaaaagc agaaataact
      181 ggctatttct agcggattat ttctgcaata actaattatc tattttttat tttcaattaa
      241 tttaaagctt gaccatggtt tcaaaaagtc aaggaggaat aattcgtcat cggagattct
      301 tcctacaaca ttgacacgtc cgtcgttttt catatccttc aaggcgattt gtgtttctcc
      361 tttgtattga ccgtaccctt cattgtcaat cagtacatcc ccatgatgca tatctcgcgt
      421 attatgaggc gggaaatctt tatctttgta gtatacacga gtcatcgttg aacgaaggat
      481 atattctgag cgatcccctc gataactatg aaggttatca aacaagcaga ttcgttcgtt
      541 ttctgtaata ccttcttccg tatctacttt caatgttgga taatccgcgt taaatgcttc
      601 tgccatttct ttcaattctt cttcacttgc gtacgcattt ccaatagaaa tatcatcaat
      661 caatcctgta aggattaaat gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc
      721 cagcgtgcac aagccatctt gcgtaggcca agggccaaac tcagcagatt gcgaattgac
      781 gaatgccatc gtattcaaat tatattttct gaatttttct gaacaaaaaa caaagtgttc
      841 gtatcctaaa ccagagtatc gatgaggata aaaattatga gaacctaata aattatccgt
      901 gtttggcgaa taactcatga tgttgtcaac gtaattcgtg cccgaactca tattgatctc
      961 gattttgata ccaaaaggat tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat
     1021 atctaagcgg accccgtagg cacccatctt atggaaaaaa gataagtcat cgtaagaaat
     1081 ctccaactgt tcaaataacg ctggattgat atcaaccatt acttccatgc ctaggctatt
     1141 cgcgtaatca actacttctt taaattctga aagaactttt tctttatcat cattgatctg
     1201 cagcaaactg gtaaaaaccc gtttataccc atacttatga gctaaatcca agtacgcttt
     1261 gtccttttca aatgttgacc gttctggata aatcgagatt cctaattttc ccatattacc
     1321 atttcctcct gcatcttttc gtttttgtat ccacttacat tatagcaaac acaaagtaaa
     1381 tggtctacac cttttctttt tcttttaaaa taaatgataa attccaataa tttaagtagg
     1441 tattttccct ctattttgtt atcattaaag ataacaaaat agtaaattgg aaaggaacag
     1501 ttatgaagaa aaataaagtt attttaagta ttagtttagc agcagcattg ctgtttgctg
     1561 ctggttgttc tgcaaacaac acgacttctt ctagttcgtc tacagaaagt tcaaaaagta
     1621 catctactgc ggaaagcacg actgccagta gcgtcgattt gaacgcattg gaattaccac
     1681 aattgacgaa agatgtcgcc gaaaatgaag atctggtcga aatggtgacg aataaaggca
     1741 caatcaaaat caaattattt cccgaacacg caccaaaagc ggttgaaaac tttatgacac
     1801 atgcaaaaga cggttactac aacggactga ctttccatcg cgtcatcaaa gatttcatga
     1861 tccaaggtgg cgatcctaaa ggcgatggca ctggagggga aagcatttgg ggagaaggat
     1921 ttgaaacaga gatcaacaac cacctttata atataagagg tgcgttatct atggctcgct
     1981 cacaagatcc taacagtaac ggcagccagt tctttatcgt tcaaaataca gatgatatgc
     2041 atgatggtct tttaaaagac gactatcctc aagcaatcat cgacgcttat aaaaacggcg
     2101 gttatccttc actagatggc cattatactg tcttcggcca agtgattgaa ggaatggatg
     2161 tcgtggatgc tattgcaaat atcgaaacag atagttcaga caaaccaaaa gaagaagtaa
     2221 aaatcgataa aatcaatata ctgcaagaag caaaacaaaa ataaaaaaat aacgtctgaa
     2281 tcctgcatag atgacaacgt atgctaggat tcaggcgtct tctttttaca taaaaagcaa
     2341 gtaagcttcc ttttgttttg ctatatcagg ttttatagtc aagcattttg gttgacgatg
     2401 aacgtgaact cgcactcaca gactttttta tcatctacat atgctgcaga taacgctgta
     2461 ccgactgatc ccttcatttt ggtgatttca aaatgacatt tcagcacatc tcctggaaca
     2521 actttttgac ggaatttggc cttattgatc ccaccgatat aagctctttt tcctagaaac
     2581 tcttctgatt ttaggataag tatcgaacct gcttgtgcca gtgtttctag aatcaacgca
     2641 cccggcatca ctggattgtc aggaaagtga ccttggaaaa aatcttcatt gattgttaca
     2701 ttttttgtcg caacgatctt ttttcctggt tctaaagtat ctacataatc aatataacaa
     2761 ataggataac gattaggaat caattccatt acttcagtag cagataatac gtaagacata
     2821 attaaactcc ttctttcagg caattgccat tttcattact ttgactatca aagtatcata
     2881 ttcctaaata tttagcaatc tttttttaat ttaagaaaaa atagtttgac aatcaaaaca
     2941 ttttgactaa taattaagaa atcaagaagt tattttagct attattctct atttttttac
     3001 gactatcaaa tttttatcag aaacaaagga gagtcattat gtcatttcta gaaggaaaaa
     3061 aaatcgtcat tatgggcgtc gctaacaaaa aaagcattgc ttggggttgt gctgaagcaa
     3121 tgttgaaaca aggggcagaa attatctata cttaccaaaa tgaacgaatg aaaaaatcat
     3181 tagtcaaatt ggtcggcgaa gaagcattca ctgtagaatg tgatgtttct agcgacgaaa
     3241 gcatcgaaaa agcaatgaat gccatcggtg aatatgctgg ggagatccac ggccttgtcc
     3301 atgcagttgc ttacgccaac aaagaagaat tgtccggaaa tgtgtctgat atttccagag
     3361 acggctatca attagctcaa gatatcagca gctactcttt aattgctgta tcaaaatacg
     3421 cacaaccgtt tcttgcacca aacagcggga tcgttagcat gacttatcta ggttcagaac
     3481 gcgcaatccc gaattataac atgatgggta ttgcgaaagc ttctcttgaa gctgctatcc
     3541 gttatttggc agctgagttc tcgcctaaag gaattcgggt caatggaatc tcagctgggg
     3601 caatcaaaac attagctgtc acaggtgtta aagactatca aaaactgatc cagttatctg
     3661 aatctcgtac gcctgatcag caaggtgtga cgatcgaaga agtcggtaat gcttgtgctt
     3721 tcttaatcag cccactttcc agcggaatca tcggtgatat tatctacgtt gacaaaggtg
     3781 tccatctttc ataaactttt gttgcattct ttaaagtaaa acatataaaa atcaggaaaa
     3841 aactttttaa agtctcttcc tgattttttc tttttacgtt tttttcaact cattactttg
     3901 aaattattcc acgacttcaa tccagccttc tggtgcttct atgtctccaa attgaatacc
     3961 tgtcaattca tcatacaacc gttttgttac tggtcctact tccgtttcac tataaaatac
     4021 atgaagattg ttattgtgat aaatcccacc gattggagaa ataacagccg ctgtaccaca
     4081 tgcaccagct tctgcaaatt gatctagatc atcaatgaaa atatctcctt caattgcttg
     4141 cagtcctagt cgttcttcag ctaactgcaa caaggaatat ttcgtgatac ttggtaagat
     4201 agagggcgat tgcggagtga tgaattttcc gtcttttgta attccaaaga aatttgctgc
     4261 acctacttct tcgattttcg tatgtgtggc tggatcaaga taaacacaat ctgagtaatc
     4321 cagttctttt gcttctgttc ctggaagtaa actcgctgcg taatttccgc cgacttttgc
     4381 tgcaccagtc cccttaggag cagcacgatc gtaatcagat acaataaaat tcgttggttt
     4441 tagtccacct ttgaaatacg ctcctactgg cgtacagaag acgacaaata aatattctgg
     4501 agctggatgg acaccaatgt tttcaccgac cccaatcagt aaagggcgca agtataatgt
     4561 agcgcctgtt ccatagggcg gcacaaaatc ttcatttgct tttaccactt gcttgacagc
     4621 atcaacgaac atcttttcag ggaccattgg catcagcaat cgtctagcac tttgattcat
     4681 ccgtcgacta ttttgttcag gtcgaaagag attgaccttg ccgtcttttc gacgataagc
     4741 ttttagacct tcaaaacatt gctgaccata atgaagcgct ggagagcctt catgaatcgt
     4801 caggtaatta tcttctgtca attctccttc ttcccattgt ccgtctttcc atttggcaat
     4861 aaaacgccaa ggagttttga tataagaaaa acctagattg ttccaatcga tttgcatata
     4921 cttaccacct tcgttatttt ctccacattt tatcattatc catattattt gt
//
LOCUS       DAJSMI010000168         4964 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJSMI010000168 DAJSMI010000000
VERSION     DAJSMI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.172"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..862)
                     /locus_tag="ON430_002435"
     CDS             complement(<1..862)
                     /locus_tag="ON430_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCR3220321.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRK"
     gene            1015..2475
                     /gene="rhaB"
                     /locus_tag="ON430_002436"
     CDS             1015..2475
                     /gene="rhaB"
                     /locus_tag="ON430_002436"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR3220322.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            2472..3728
                     /gene="rhaA"
                     /locus_tag="ON430_002437"
     CDS             2472..3728
                     /gene="rhaA"
                     /locus_tag="ON430_002437"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR3220323.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            3742..4587
                     /gene="rhaD"
                     /locus_tag="ON430_002438"
     CDS             3742..4587
                     /gene="rhaD"
                     /locus_tag="ON430_002438"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR3220324.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            4589..4900
                     /gene="rhaM"
                     /locus_tag="ON430_002439"
     CDS             4589..4900
                     /gene="rhaM"
                     /locus_tag="ON430_002439"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR3220325.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
BASE COUNT         1596 a          841 c         1147 g         1380 t
ORIGIN      
        1 atttacgaaa gtctctcggc gatagtcctg tttttgcttt gaacaggcgg ctaaagtaag
       61 caaaattctc aatccctatt tcttcggaaa tcattcgaac cgtaaattct gtataccgaa
      121 gaaggtcttt agcgtaagaa agttttttgc tgatcaaata atcgattggt ggagaaccga
      181 taaatttgga aaaatctttg ctcagctgat atttattcaa taagaattgt tgttctaatt
      241 gatcaagtgt aatcgtttct ttgaaatgtt cgtctaagta ttgcttcagt ttgtaaacgt
      301 aggcgggaat atcctcttct tcgaaatcta gctgatactt ttgcaaaatc agttcattca
      361 gcaactcgtg gatagctaca gaggactgga aatcggtacg ggcattcgtg ccttgttgca
      421 tgtggatcaa ttctgtgatc aaatggtgga tcttgttttc tgatggatcc cctgttgaat
      481 ggaaggtgtt atggccgtct tttgtgaact cctgataaaa tgattggacg ttctgaccat
      541 tcaaatggat ccaatccatt tcccatggtt cttcgctgat cgtttgataa cgctgatatt
      601 cttggcagtc aataaaaaat aagtctccag cttttagctg ttgctgatta tcattatatg
      661 taagcgaacc tttgccagac aaagtaaact ttatcaagta agaaggtaaa tgtgctcgtt
      721 cagtatagta agggattggt gctttgaagt aaccgatttc ttggatataa aaaaataaat
      781 tcctgcttct ttgtgtaggc gtcaagatca ctctttttga gtcttccgac catgagtggt
      841 tggctttttc ccagtaatcc atgtaaacac tcccaacttg tcaagataat gcaattatat
      901 accaaaatag tgtgaagcaa aatataagat tctgtattat gatagcattg ttaataaatg
      961 ataacgcttt attaataaag tgctaatcaa caataaagta aaagaggtgg agaaatgaat
     1021 ccaacatttg ttgccatcga tatcggtgca tcaagcggac gactaatgaa aagtacgagg
     1081 gatgcaaaag gttctttgat cttggaagaa gtacaccggt tcaaaaacgg ctttcggaac
     1141 aaagacggtc acgatcgctg gaagatcgat tatcttatca aagagatttt gattggattt
     1201 gaaaaattaa agcagacagg gattgatcgc ctttttgcgg ggatcgatac atgggcagtc
     1261 gattactgtc tgattgatga acaaggggat cggataaatg atccgattgc ttatcgggac
     1321 aagcgtacca atggagcagt agaacagttt tctgaaatct attctctaga taagttgtat
     1381 gagcagacag ggattcagat ccagcctttc aataccttgt tccaattgtt tgctgaagaa
     1441 aaagaaaatt tggcaaaagc acataaattg ttactgatac ctgattattt agggtatgtc
     1501 tttactggta acatggtgac ggagaaaact aatgcatcta ctatgcagct gctgaatgca
     1561 gggacaaaag aatgggaaat agaacttctt gaaaaaatcg gtgtctcgaa ggaattattt
     1621 ccaccattga cagagccggg aacgatacta ggagatctgc aaaaagaaca attttcaaaa
     1681 tatgatttgc cagatgtgac ctttatcaca gtagcaagtc atgatacggc gtcagctgta
     1741 ttaggaacgc caggtcaagg agaacgttgg ggttatatca gcagtgggac atggtcgctt
     1801 ttagggattg aaacaaagat cgccaatgtt tctcgcaatg ctttttctga aaattatacg
     1861 aatgaatggg gtgcccataa cacggtgcgt tttttgaaaa acatcatggg gatgtggttg
     1921 atccaggaag ttgcccgaat gcaagagtac gagtacagtt atgcggaatt ggcagagctt
     1981 gctgcaaaag aaaaaccgct gcagcaagtg atggaaatca atgaccctcg atttttgaat
     2041 cccccaaata tgatagaaga gattcagcaa tactgttttg aaacagagca gccagtccct
     2101 gtctcatcgg gagctatcgc tcgttgtgtg tatgataatc ttgctctttg ttacgcacaa
     2161 gagctgatca aagtagaaca cttaactgga acgaaattgg aaaagctgca catcgtaggg
     2221 ggcggatcga ataatcgtct actgaatcaa ttgacagcag attatgccgg aatcattgta
     2281 gaagcaggac ctagtgaagc cacagcaatc gggaatgttg ccatgcaaat gctcagtaca
     2341 ggtcagtttt cgaatataga acaagcaaga aatgtaatca aacagtcgtt tccatgtgaa
     2401 actttttacc cagatgaaaa taagcaaaac acgatggaat ggtacgtgaa taaaatcaaa
     2461 ggagtaatcg tatgaacacg attgaaagaa attacgaaca agcaaaagaa aaatatgcaa
     2521 ctatcggtgt agatacagat gctgtactag aaaagataca ggacatcaaa atttctatgc
     2581 attgctggca aggagatgat gtcaaaggtt tcttgacacc ggatggcgaa ttgaccggtg
     2641 gaatcatggc gacagggaat tttccaggag ccgcacgaac acctgaagag cttagacaag
     2701 atctggagaa ggcttattcg ttgataccgg gcaaacacaa attgaacctt catgctatct
     2761 atttggatac agaagaatct gtcgatctaa atgaaattga gccgaaacat tttgaaaaat
     2821 gggtagagtg ggcaaaaaaa gaagaaatcg gtcttgactt caatccgact ttcttttcgc
     2881 atccaatgat gaaagacggc tttacattgg ctcatccaga taaagaagtt cgtgaatact
     2941 ggatcgaaca cgggaaaaga agccgcaaaa tcgcggagta ctttgggaaa gaaactgggc
     3001 aaacaagtat caataacttt tgggtacctg atgggatgaa agataatccg attgatcgtt
     3061 ttaccccacg ggaacgttta atggaatcac tcgatgaaat ttttacagaa gagttagatg
     3121 aacggtatgt acaagatgca gtagaaagca aactatttgg tttaggcgct gaagcttata
     3181 cagtcggttc ccatgaattt tatatgggtt acgggttgac tcgtgacaaa cttgtttgtc
     3241 tagatgctgg acattttcat ccaacagaag tgatttccaa taaattatct tcattagctt
     3301 tattcagcaa aggaatcatg ttgcatgttt cccgtccagt gcgttgggat tcagaccatg
     3361 tggttttgat ggatgatgaa ttgcaggaaa tcgctaaaga attagtccgt aacgaattgt
     3421 tggaaaaaac aaatatcggt ctggatttct ttgatgcaac gatcaaccgt atcgctgctt
     3481 gggtcatcgg tacgagaaat acacaaaaag cactgctgaa agctatgctg gaaccagttg
     3541 agcgattgaa agaaatggag ctggcttttg actttacttc aagaatggct tatacggaag
     3601 aattgaaaga tttcccgtat gccgatgtat ggaattattt ctgttacaaa aatcaagttc
     3661 cagttggttt ggattggctg gaagaagtcc aggaatatca aaaagacgtg ttggaattaa
     3721 gaaaatagga gtgtggctat attgaaaaaa atagatattt tacaagcgcc ttatgtgaaa
     3781 gaaatgatcg aaacgacgtc gaatctttat cgcttgggat gggatgaaag aaatggcggg
     3841 aatatttctt atttactaaa agaagaagag atcacaccgt tcttggatcc tgaaaaagtg
     3901 atccgccgca tctcgatgat ttttgatgcc agccgtttag caggatgcta ttttattgta
     3961 actggttcag gaagatattt taaaaatgta gcgagcgaac ctgccgagaa tcttggaatc
     4021 gttcgagttg ctgaagatgg caaaacactg gaacttttgt gggggctgga aaacgaatgc
     4081 cttcctacaa gcgagttgcc aagtcatttc atgagtcata tcgcccgttt agaagtagac
     4141 ccagataatc ggatcgttat gcattgccat gctagtcatc tattagcgat gagttttacg
     4201 catgaattag acgaacgttc ctttagtcgt acgctatggc aaatgtgtac ggaatgctta
     4261 gttgtttttc cagaaggaat ttccatcatt ccgtggatgg ttccgggaac aaatgagatt
     4321 ggggaagcaa cagctgaaaa aatgaaagaa actcgattgg ttttatggcc acaacatgga
     4381 atctacgggt caggaaaaga catggatgaa gtctttggtt tgatcgagac agcagaaaag
     4441 gcagcagaag tctatacgta cgtcaaagcg caaggaccaa tcttgcaaac aatcacggac
     4501 gaaaacctgt ggcatttagc agatgcgttt ggtgtcactc caaaagcagg ttatttggag
     4561 gaagttcata cgaaagcagg tgtctgacat ggtgaaaaaa gctttttgca tgaaagttta
     4621 cccagaaaaa caagaagaat acaaaaaaag acatgatgaa ctttggccag aaatgcgtac
     4681 agcacttcgt gaacacgggg tcatcgacta ttcgatctat cttgatccga gtacaagttt
     4741 gttatttggt tacttagtga tcgaagatga aaagttatgg gaaaaaatgg caaatacatc
     4801 tatcaatcaa aaatggtgga agtacatgga agatatcatg gagacaaatc cagatgcctc
     4861 tccagtcagt tgggaattga tgccagtctt tgaactgtag caacaagcaa ctgggcaacc
     4921 ggttgcttgg ggttctttcg tttttattgt gaaaaataat acaa
//
LOCUS       DAJSMI010000169         4887 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJSMI010000169 DAJSMI010000000
VERSION     DAJSMI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.173"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            22..495
                     /locus_tag="ON430_002440"
     CDS             22..495
                     /locus_tag="ON430_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3220326.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            591..1478
                     /gene="xerD"
                     /locus_tag="ON430_002441"
     CDS             591..1478
                     /gene="xerD"
                     /locus_tag="ON430_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR3220327.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            1497..1868
                     /locus_tag="ON430_002442"
     CDS             1497..1868
                     /locus_tag="ON430_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3220328.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            1838..2635
                     /locus_tag="ON430_002443"
     CDS             1838..2635
                     /locus_tag="ON430_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCR3220329.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            2619..3188
                     /gene="scpB"
                     /locus_tag="ON430_002444"
     CDS             2619..3188
                     /gene="scpB"
                     /locus_tag="ON430_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCR3220330.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            3194..3910
                     /locus_tag="ON430_002445"
     CDS             3194..3910
                     /locus_tag="ON430_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR3220331.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      3992..4117
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4240..4851
                     /locus_tag="ON430_002446"
     CDS             4240..4851
                     /locus_tag="ON430_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3220332.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
BASE COUNT         1639 a          843 c         1076 g         1329 t
ORIGIN      
        1 taaattgttg gggttgagta aatggtcatg aatgctattt cggcgatgaa aaaaacaaaa
       61 cagcagctac atgaagcggg gtttaagctg acaccgcaaa gagaagctac tctgcttgtg
      121 cttttggaaa atgagaaaga gcatttatct gcagaagaaa tcttcttttt agtgaaaaaa
      181 aagagtccgg aaattggact tgctactgtt tatcgaaccc ttgaaatctt gacagaattg
      241 aaaattgtcg ataaagtaag ttttaatgat ggattagccc gttatgattt gagaaaagaa
      301 ggcgccaaac atttccatca tcatttacta tgcttgaact gcggcacgat cgaggaagta
      361 gaagaggatc ttttacttga agtggagcaa gtagtagaag aacgttatca ttttttagta
      421 aaagatcatc ggctaacgtt ccatggaatc tgtcaggaat gctatgaaaa agaaaaaaga
      481 aaccagtccg tctgagtgtc tagacggctt tttctttgct cagattatgg aaaaagacta
      541 tcagaatgaa gcggtatctg atatgataga gaggcatgag ggggattatt atgaaagagc
      601 aaatcaacga ttatctccac tacttgaata ttgaacgggg attgtccgcc aatacacgac
      661 aaagctatga acgtgatttg gaacagtact tgcattttct acaggagcag aaaattgctt
      721 catggcagga tgtcgatcgc tttttagtca tccgttttct ggagaagttg catgaagaaa
      781 aaaaagcgac agcaactgtt actcggatga tctcaagtct cagaagattc catcagtttt
      841 tacggcaaga gcgtatcact gatcatgatc cgatgcaaca tatcgatacg ccgaagaaag
      901 cacagaaatt accaagtaca ttgactctga cagaagtaga aaagctgatt gaagcacctg
      961 atacgacaaa aacgttaggg ataagagatc gtgcaatctt ggaagtcatg tatgctactg
     1021 ggatgcgcgt cagtgaattg attggattga aattgggaga tcttcatctt tctcttggac
     1081 tggtacagac agttggaaaa ggagataaag agcggatcat tcctttaggt gattatgcga
     1141 tccaatggct ggaacgctac ttagacgaag cacgtccatt acttgtaaag gacgcatctg
     1201 aaatgcatgt atttgtcaat aatcatggga aaggtctttc tcggcaagga atctggaaaa
     1261 atctgaagca gttagtacga gaggctggga tttacaaaga agtaacacca catacgctgc
     1321 ggcatagctt tgccacacat ctgctagaaa atggagctga tttgcggaca gtacaggaat
     1381 tgttaggtca cgcggatatc tctacgactc agatttatac acatatcacg aagaaaagaa
     1441 tgacggatgt ttataagcag cattttccaa gggcttagtc ataggaaggt gagaagatgt
     1501 taacacatta ccggaaagaa aaccgtaaaa ttgctatggg actattgagt ttccataaga
     1561 aactgacagg taaaacttct ttgttgaagg agattgatac gtacgaatct gaagacgatt
     1621 atgaactttt tttctttacg ccagaaggtt caaccaacat tcaagggatt attggtattt
     1681 actgggcaac gaacgatcag ctgatcatcc atgacatttc tttgaatccg tcttatcgcg
     1741 gtgagaatat gggctttcaa atgttagatg aattacagga aaagtacccg gatgtctcca
     1801 tccgtgcaac tgaaatcaca gagccttaca ttggaaaatg gctagcaaag aaaaagaaga
     1861 aagagtgaat gagttgacgg agatcaatat taaattagat gtattcgagg gtcctctcga
     1921 tctgctgctt catctgattc agcaaatgga aatcgatata tatgatatac cgatcgctac
     1981 gatcacggag caatatatga attatatcca tacaatgaaa acactcgagc tagctgttgc
     2041 aggagaatac ttagtgatgg ctgctacatt gatggcaatc aaaagcaaga cattgcttcc
     2101 tgtccaagaa ttcgtcacag acgatgaaga attatgggaa gaagatcctc gagaagaatt
     2161 ggtcactcag cttttagaat accgtaaata taaatatgca gctgaaagac tgacagaaaa
     2221 agcagaggaa cgaagtcttt attacacaaa agaacctatg aacatcgatg ggttagcgga
     2281 tgctgaaaag ccgttgaagc gaggacaagt caaaaaaatc gatttgatcc gtgccatgca
     2341 gaaaatctta gaacgtaaga aattttctca aaaagtggaa aaaacgatca cgcccgatga
     2401 cacaacgatc gaagaacgaa tggtatttat cgaagagcgt ataaaaagaa atgcgcatgg
     2461 ctgtactttc gattcttttt ttgagtcacc ttctaaaagt gaagttgtca ctacttttat
     2521 ggctttgcta gaattgatga aaaaagggca gatcattgcg caacaaaaag aaaactatga
     2581 atcaattatg ctttatccag cagtaggaga taccgaacat gacgaaatta agtgaacttg
     2641 aagcccttct ttttattgtt ggcgaggaag ggatcagtaa tgaagaatta agcggactgt
     2701 taaatctcag cgaagcagaa gtcacaagtt tagtgaaaga attaaaagaa tcttacactc
     2761 aaaactcgca ctcagctttg catatttttg aaacggaaag ccattatgta ttaacgacta
     2821 aaaaagagct ggctccctta cttaagcgat atgcgcaagg gacatcaaac acattatctc
     2881 aagccgccgt cgaaacctta gcaatcatcg cttataaaca gccaatcaca agagctgaaa
     2941 tcgagcagat tcgaggagtt caggtttcag gagcggtaca gcgtctagct gcccataaac
     3001 tgattgaaga aaaaggtaga gtagaaggac caggtcggcc gatattatat ggaacaactg
     3061 gctacttctt agattatttt ggtttgaaat cattagaaga actgcctgat acacaagaaa
     3121 tggaaatttc acttgaagaa gacgtgtcac cagatctatt ctttgatgga atgaataaag
     3181 gagaataatt gtaatggaga gattgcagaa agtaattgcc cacgctggca tcacttctcg
     3241 tcgaaaagca gaagaattca tccttgcagg acgtgtaaaa gtaaacggac aagttgtaag
     3301 ggaattaggg ataaaagttg ggaaacatga cactgtggaa gtggatgaag tcccaattta
     3361 tcaagaagaa tacggttact atttattgta caaacctaaa ggagttattt ctgctgttgc
     3421 agatgacaaa ggaagaaaag ttgttacgga tcttttgccg atggtaaaag aacgtatcta
     3481 tcctgtaggt cgactggatt acgatacttc ggggatcttg ctactgacaa atgatggaga
     3541 ttttgctcag cgcctgacac atccaaaaca tgaagtggat aaagtttacg ttgcaaaggt
     3601 aaaagggatc gctacaaaat cgatgcttgc acctttgaca aaaggaataa agatcgaagg
     3661 acgaaaaaca tctccagcta aattccaaat tttatcagtg gatcctaaga caaaccacag
     3721 tatcgtggaa ttgacgatcc acgaaggacg taatcatcaa gtgaaaaata tgcttcaagc
     3781 agttggtctt cctgtgcaaa aactaaaacg tgaaaaatac ggagatctga ctttacaagg
     3841 cctacgtcct ggggattacc gtgaactgaa caaaaaagaa gtcagcagtt tgctgaacca
     3901 ctcaaaatga acacttactt tttataagtg ttagatgttg cgttttttga atagatatgt
     3961 ataatgaata tgtaaacaaa aattatataa ggttcgtctt caggggcagg gtgaaattcc
     4021 cgaccggtgg ttatagtcca cgacctattt tgcaaaaaaa tagctgaatc ggtgaaattc
     4081 cgataccgac agtatagtct ggataaagaa gatagagctt atttgacgac gcttgatcaa
     4141 ataactctaa ctctattttc cctggaaaat agagtttttt catttggaga gaaacgtcaa
     4201 aaagttcgct gaagaggagt cctaacagga ggatttcaaa tgaagaacac ccgtgtacag
     4261 aaaatggttg cagtagcgat gtttgctgcg atcgcattag ttttacagta ttttgctttt
     4321 ccgatcatgc cggcttttag ttttttgaag atcgatttta gtgatatctc ggtattgatc
     4381 agcatgttct tattcggtcc agcggccgga gttgcaacag cgttcttgcg ttcagtatta
     4441 catctgctga ctacaggttt ttctccggat aatttggtag gagacactgc tagtttctta
     4501 tcaacaacga tctttacatt cccgatttat tatttcttta gtgtaaagaa acagcataaa
     4561 ggaagaaaca aatttttagg tgtcattaca ggaaccatcg ctctaactat ttttatgagt
     4621 atcgcaaatt atttcgttat tacgcctctt tacttaatgt ttttagggct gaacgcaaca
     4681 cagatgctgg gcatgtcttt agcaaactat gtactgattg ggatcgttcc tttcaacttg
     4741 atcaaaggat tcatcgtgag tgctgtcttt ttagtgctgc atgcgcaact tcttccatgg
     4801 ttaagtcgaa aacaacatca gttcgaacaa cgtcatacag cagtcaaata aatatcaaaa
     4861 agatcaagaa atttcttggt ctttttt
//
LOCUS       DAJSMI010000170         4877 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJSMI010000170 DAJSMI010000000
VERSION     DAJSMI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.174"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(75..764)
                     /locus_tag="ON430_002447"
     CDS             complement(75..764)
                     /locus_tag="ON430_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR3220333.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(809..1573)
                     /locus_tag="ON430_002448"
     CDS             complement(809..1573)
                     /locus_tag="ON430_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCR3220334.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(1601..2299)
                     /locus_tag="ON430_002449"
     CDS             complement(1601..2299)
                     /locus_tag="ON430_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCR3220335.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2311..3090)
                     /locus_tag="ON430_002450"
     CDS             complement(2311..3090)
                     /locus_tag="ON430_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCR3220336.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(3106..4050)
                     /locus_tag="ON430_002451"
     CDS             complement(3106..4050)
                     /locus_tag="ON430_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3220337.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4096..4875)
                     /locus_tag="ON430_002452"
     CDS             complement(4096..4875)
                     /locus_tag="ON430_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220338.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1256 a         1042 c          857 g         1722 t
ORIGIN      
        1 cagttattac ttttgaaacg ataatatgaa catgtgtaat cttttttatt aacaatccat
       61 cattctatcc ctaactaata tgctccattg ggcttgacaa taacataaaa cgtttttaaa
      121 atcaacctca agtcgtacat taaacttcga tgactaatat actctaaatc caactcaacc
      181 attttatgga aactaacatt atttctatca ctaacttgcc acaaacctga acaaccaggt
      241 tttacataca agcgttgaag atcatatctc gtgtactcag ctacttccct ctccaaaggt
      301 ggccgtggac caattagact catatcgcct tttaaaacat tccatagctg tggcaattca
      361 tcaatgcttt tggcacggat aattttacct attttagtga cacggggatc ttctttcatc
      421 ttgaacattg caccttctat ttcattttga tcaagtaatt cttccaattt ttcttcagca
      481 tctaaacaca tcgaacggaa tttatacatt ttgaatgttt ttccattttt accaacacgt
      541 gtatggcaaa aaaatatcgg acttcctggc tcttcttttc tgattcgata agctacatag
      601 aacatcaaag gagaagctaa aatcaaacca ataatgcttc ctacaatatc aatcattctt
      661 ttaaaaaata aatagatata tcgtttttca actttttctt tatccaacca aacttgatta
      721 ggatgtactt cactaatacg agataataaa tcttcacttt ccatactttc cctctctcct
      781 catttccttt ttatctacga ataaagaact aaaataaacc aaacttcttc ttttttacct
      841 cttcatagac tggataactg atttcatccc cgttgatcag atcctttgcg acttgtttca
      901 tctgtactac tttttcctta ccaaagtcct tagcaatctg ctcatagcac tcttttaagt
      961 aaaaggtacg cttagagacc ccatgggcat cagaagcagc catctgcacg agattatgtt
     1021 tgaccattgt tttggctgtt ttctgaatgt ctttgccaaa tacaccaaca taacttggcg
     1081 cagtaatttg ggccaaacat cccatatcta ggaacggaag caaatgattt gggtcttctc
     1141 gaaaatgcgc gttgcgttct ggatggacga tcactggtgt ctttcctagt tctaacaact
     1201 caaaaaaaag ccattctgca tataacggca cttccagtgt cggaaattcg atcagaagat
     1261 atgtatcatc taaatctgta aataaaattt catctcggtg gatgtcttcg atgagcgttc
     1321 cggtaatacg tacttcttgt ccttccaata acgtcaaagg caactgtctt ttctctaatt
     1381 cggcttgtaa cgaagcgact agagaaatga cttgtgattt ttcattgctg tattttccat
     1441 tattgtggtg tggtgtgcat aaaatatgcg tgatgccttg ctgaatcgct ttttcagcca
     1501 ttgcaatact tgcttctaag ttttctgccc cgtcatcgat tcctggtagg atatggcaat
     1561 gtagatcgat catttctctt gtctcctctc ttcttattcc ttaatttccg tagtaataac
     1621 cagcatcttt gctatgttct gctccgttgt atacaacacc taaaactcgt gcttgtacca
     1681 tctctagcag ttcttttgct ttattcaatg attctttacg tgatgtattt tcacgtacga
     1741 ccaataaagt gccatccgtt tttgaagaca tgatctgtgc atctgttacg gctactactg
     1801 gtggcatatc gaatatcacc acgtcaaata actgtcttgc ttcgtctagg atctgatcca
     1861 ttcttggcga acttaacaat tctgatgggt tgggtggctt aggtccacta ggcaaaatcg
     1921 acaagttctc gactggcgtt cgttggatta catctgctac acttcctgat gaactaagtg
     1981 ctgtacttaa tcccctggca ttgttcagct ggaatgtctt atagacgacg ggtttacgta
     2041 aatctgcatc gactagtaga actcgttgac cggatttagc aaaaacgact gcaatgtttg
     2101 ctgcagtggt ggattttcct tctccaggac ctgatgaagt cacgacaatc gttttgattt
     2161 gctgaccagc tgcggaggca aactggatat tcgtccggat cgtacgatat tgttcggcga
     2221 ttggtgaaga tgggtttgtt aatgtgatca aactgactgc atgagtttgt gctgcttttt
     2281 gtttctgtgt tcgtgccatt tttcttcctc ctagactctt gagcggcttc tgcgtgatgg
     2341 ggctgtttta tcgtttgtag cttttggtcg tgcaggagct gttccttttt tacggatacc
     2401 agcgttcaat tctttcgctg acatttctgg aactgttcct aagattggga atcctaatgc
     2461 atccgtcaca tatttttcat ctttcactgt ccgatcaagt aattcgaata agaatgccag
     2521 acctacgcct accatcattc ctaatactaa gccaatcact agattcaatt tattatttgg
     2581 cgaaacaggt gtcgtattgg caactgcacc tgaaataatc gagattttat cgacattcat
     2641 cacgtctttt gcgttctctt ggaaaacttg tgaagttgtg ttagcgatct gttgagcagt
     2701 cactgcattc gtgcttattg cctggatcga gaacatttgt gaattttgtg actgattgac
     2761 ggagatcgca tctttgattt gtccagcagt cattttcaca ttgtcttctg tttctaaacg
     2821 atctttgact tcatttagta ctaaatcact gacgatcata tctttatacg tattgatcat
     2881 ttgcaggttc gtattgacat cattgacatt tgctgtgtct gattgtggca gtgtcacgat
     2941 caattgtgct tgtgaactgt attttggtgt caagatgaaa aatgttacga ttcctgagat
     3001 ggccaagcca gcaaacattg agatgatgat tgttaccaag tgtttgcgta atgtttcaaa
     3061 aatttcttcc aagttgattg tctcttccat tttttctcca ctttcctatt tattctctag
     3121 attcatttga tcttttaatt ctttttttac acgatcaagt tcttgatcac tgactttttg
     3181 ataggagata ttatcttgca taaagccttc tccttgcagc tgatcttgct tgatcgtacc
     3241 aaatgcctcc cgataatcta aagcgatttt tttcatttca tcaaagctca tgtctgtttt
     3301 catattcgac tctagagcat tcaaaacgga ttgatagttg ctgacaccgt ttaaactcaa
     3361 tacttttttt gctacacctt ctacgatctt tctttgacgc tcttgtctac catagtcacc
     3421 atttggatct tcataacgca tacgagaata agataatgcc tgatccccgt tcaatgtgat
     3481 tttaccgatt gtaaagtcat agccatcttg tgagaaagtg aggtcattat tgacttcgat
     3541 accaccaact gcatcaacaa gttcctttag ccctttcata ttgatagaga catagtgatc
     3601 gattgggatg tctacatatt tttgtacggt atccatcgcc atggcagctc cgccgaatgc
     3661 gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg atgtcgacat acgtatcacg
     3721 aggaatgctg acgatcgttg tctgtttgtc ttcaggactg actatggcaa ccatcatcgt
     3781 atctgatctt ccttgatcga cccgacctaa atctccagta tcaatcccca tcaatagaac
     3841 agagaaagat tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt gggttcgttc
     3901 cactgattcg taagttttct caatagaatg tgagagatcc agatataatt ttgctcctac
     3961 tcctacaatt gctaatacta atacgataag aactgctaaa ataatcagaa atattttttt
     4021 ccctcttgat tttcttcttc gtcttgccat agtttcctcc gtttgctaac cttgcttgaa
     4081 tcaacgtttt aactgtcaat aacttatatt gctatagccc atttgctgag caacagtttg
     4141 tgcttttgtt gcgtatgctg cgcttatttt ttcattccgg atctgctcta tttgactgag
     4201 cgccgtcaaa tattgatcgt aagtagcggt ggcagtcact tgaccatcct tcaacatcgt
     4261 atcgaataac tgatctagtt gcgtgacttg cgcgatctga gtatcagcga atcctaaata
     4321 ttgcataaca accgttttcc aaggactatc tttaaaaaag ctcatatttt cacgtacacg
     4381 agcgacatct gcacttgtca ccttttcttt gatgatgaca tcgtctacag cttgctgcca
     4441 gtttgataca tttttagtaa acaaatcgtt gacagctgtc tggatcttca gttttgattc
     4501 tgcactatct aactgtgtca cgattgcttt cttctcatct tggattgcaa cagcttcttt
     4561 aggcaattct gattctttga tgtcgtaatc atctgctgaa agtttgacat aggatagctt
     4621 atttgacaaa cttgtgattg tttcttctgt tatttcaggg tttagaaaaa ttttttcttt
     4681 tgtactatac agcttgtcta cttcttttga taaagaagtc aaggtgtctt tttgattggt
     4741 gatggcagat tctgcataat tgatccgttg ctgatgttgc cgctgattat tgatatggta
     4801 accaaacaaa cagatcgcaa tagcacctgc acctaccgcc acagcagtca atgacttgac
     4861 gaatggcttt tccatag
//
LOCUS       DAJSMI010000171         4761 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJSMI010000171 DAJSMI010000000
VERSION     DAJSMI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.175"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(286..1011)
                     /locus_tag="ON430_002453"
     CDS             complement(286..1011)
                     /locus_tag="ON430_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220339.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1165..1491)
                     /locus_tag="ON430_002454"
     CDS             complement(1165..1491)
                     /locus_tag="ON430_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220340.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1528..1893)
                     /locus_tag="ON430_002455"
     CDS             complement(1528..1893)
                     /locus_tag="ON430_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR3220341.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1930..2145)
                     /locus_tag="ON430_002456"
     CDS             complement(1930..2145)
                     /locus_tag="ON430_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3220342.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2274..2609
                     /locus_tag="ON430_002457"
     CDS             2274..2609
                     /locus_tag="ON430_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3220343.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2685..4682)
                     /locus_tag="ON430_002458"
     CDS             complement(2685..4682)
                     /locus_tag="ON430_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220344.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
BASE COUNT         1502 a          929 c          613 g         1717 t
ORIGIN      
        1 tacatatgat tttttcataa aaaaacagta ctgtctagta gtataaaata tacaaatttc
       61 ttaaaaattt tttcactaaa atcactacct gaaacagtct aaaacactta tatatcaacg
      121 tttaatgaaa ctatctccgt ttcatttcag atacaaataa atatagtcgg gctccaaatc
      181 cgaaaaaaaa tacccccagt agcttatcct aagctactgg agatgtataa gtacggtcat
      241 ataacaacgg ggtagggtaa ataaatgaaa aagatggaag aatcattatt caataacttt
      301 tttaaatact tcgttgtaat cagttacctg tccgttctta aataccacag aataatcttt
      361 tttcccattt tcaagtaacc gaagtggaat atctttacca actttctctg tcactgcatt
      421 actgatacct gttagattag ctaataaatc tgataaagat tgagcatctg tagaaatctc
      481 tgtatcagta tttatcgtga ttcctttatc ttcatttaca agtttccaag taggtggtat
      541 agctgtgtat gtttctacaa atgaattaag tttttcatta agtgacttct cttttatatt
      601 tccatctaaa aactctgtat atatggcttg agctatacta aaacaaagct ggccttgttc
      661 taataatttt acatcattag ttagtaaagc tgtgttcacg ttatcaatcg cttcaagaag
      721 agtatctttg gtgtccctaa cagctttagg tgtgccttct ggaatttccc tacttgatac
      781 ctcgtccatt ttatcttcca taaacccctt aaaatcatat ctttctccca aatccataga
      841 agtttttgag aaagctttca tttctgagtc tatttcttca ataatattta acatgtctga
      901 tacatattgt ttaggctgat tcgtttttaa tctatatgtg gtaaatatct caattcctcc
      961 aattatcaat aaaattgcca aacaaatcca taataatttt ttctttttca tttttcctcc
     1021 tatacactgc tgttataaca gtgactaatg taaagatttt cccagcaatc atcagactat
     1081 tatttacagg gaatcaaaaa aactccttag taagcttaca cctttaggag ttcctgtttg
     1141 aacggtatgc ataatacatt taaatcaatt taacattaac acctcagtat atggtatctc
     1201 tacatataca cggtcttttt ttggatagta ctcgacattt atattgatag cacttccatt
     1261 gtctaacgta gctttagccc ggtacgtgtt atcaccatta tcatgagtga tcgtcacatc
     1321 atcacatgtc cgatttaagt tatactgttc ttgtaaaatc tgagttacca atggtacaga
     1381 tgacttctct agtccagatg aacccgtcat tcttgaaaaa attataccta taatagccaa
     1441 tacaacgata cctataatga caaacttctt attcatattt actttattca actttttatc
     1501 tcctatctcc aaaatgattt tctctcatca gtaactcatt gacatcccca atagtacacc
     1561 aaagatggct ccagctaccc ctgctatcat caaaatcgta cccatttgtt catctttctc
     1621 tcgatataag taacccataa taacagcaac agcaccaaat accacaggaa taaatgctaa
     1681 tgatactacc atagaaatcc aaccaataat caaatacagc ttccctgtct cttgtccatg
     1741 tacgatttgt tccttcattt cattaattgt ctgctcagta cttttcttta ctgtgtctgt
     1801 tccggttgtc tcatctgttt cttgtaatgc tccgcaaaac tggcaatact tactttcatt
     1861 tgttatttct ttaccacatt ttctacaata catgtcttct cctcctaaaa tcattcaatt
     1921 gcttattagc taactgttta ctttattaaa tacatcagaa aacagctcat ttattggtac
     1981 acttaatatc tcagaaagca ttttcatttg atagggttta ggaatcgttc tccctgtttg
     2041 ccacgaactt acacattgct gtgtaacatt catttttttg gctaactcag tttgggaaag
     2101 tcctttttct tttctgtatc ggctgagatt acttactttt ttcatgattc tcccctcctg
     2161 ttttattata tacaacaata tgatgtattt caactcataa aatatataaa tacttcaaat
     2221 gtcattacaa atacaacaac ttgttgtact ctactgagaa ggggggtgta ttaatgaaac
     2281 agaagcaata tgcaaaggtc attagagaaa agcgaaaagc actcgggtgg acacaaaaag
     2341 aattagctaa aaaaatcttc tctacacaac aggcagtagc aagatgggaa aattctgtaa
     2401 cagaacctaa cctcgatagc ttaacagctc tttctagagc cctaggaacc cctgtaagcc
     2461 attttctaga caatgttgac atagattatg aggaagagtt tttggctctc tatcgctctc
     2521 ttagtactga agatgcaatt cgaacaattg actatatgaa gctattaaaa aggcaagaaa
     2581 atgaacgcaa tcagctattg aaagattgaa aaataaataa cccaccgaac tttaacggtg
     2641 ggtgtattga gatgaaataa gattaagagc tatggataac ctgtctagtt taatataatt
     2701 gggtctcttc tattccttac tctccgtcca tcatctcttg ttacacttgt aaattgataa
     2761 ataggaacta attcaactcc tgataatatc aattctttta ttacttcatc tatccctgcc
     2821 cctttcgcta gatatactaa aaaactactg cttgctttca cattgtatag tttcttaata
     2881 tccttagaag ctcgttcaag agtgaatcct tgtttaacaa agtagcctct gttttctatt
     2941 aaagcatttt tcacgctgtt atccctgaca tttcgtaggc gttcacaaac agtgttaata
     3001 agttcttttt ctgtcattag atatgtccct tgccacgata agccctgtaa aacttcatgt
     3061 gcttcatcta aaatcaattg tttttccttg ctaagttctg atcgggaatt ttgaggaaat
     3121 actcttttag cctctttttc gttaaaacat atcttaaccc attcataaga aaaatattga
     3181 tatgtaaaat gaaatttttc cagaagtgga aggatatctt cattcaattt ttgaatcaca
     3241 gaagttgcat taaaaacgtc taatcgatag acattgcttc tcttatgttt aaagtttcct
     3301 aacgctttct cttcctttaa cctatttagg tattcgagca tcttcaaagg aacatcttca
     3361 tcatctaatt tttttatcaa tcctataaaa gtcataagtg atagtaactt attacattta
     3421 tctatgtcat aagttttacc atagtaagaa ctcatttttt tagctaattg ttcccccgac
     3481 aaaatacaag tcacactttc cttactatca tcaaaaaaat taacatcaga aagcaagaca
     3541 tcatagacac gtcgcatttc ctctagataa ttccttattg tcctgaaaag tttgtctttc
     3601 attgattcaa gagtgtcaaa aagtactttt gattcctctt tcaattttcg aaaatgtgta
     3661 ggatacaagt ctaggttctc taatagccaa ttaaatgttt ctacccgact gtctttcttt
     3721 aaaattagca tcacaactcc aataaaattt tcactgatat cacaattact ggaatgacaa
     3781 taaaaaatgg aatgcccagt tttactggta aaaatactag cagagggatt atgatcgttg
     3841 tgaaacagac atcttaagtt tttacttgac ttaagtaatt tattcatatc aatggttaac
     3901 aatctttcat aaatctcatt aaaagacatg tcggaggaat caaacaggca gtctttaaac
     3961 cactgtttca tttcttcttt gtcgtcattt tttatcaact taacaaaatc ggtttcattg
     4021 actagttcat actctctccc tgaatcactt tgacaatttt tagaacatgt catggtaaaa
     4081 tcaatcggaa gctcagtaat atctaacatg ttgtcttcat taattattat cactgcatct
     4141 gacttatatc ctccaaaaaa cattctattc gaagcattta cattgtcatc acacccgggg
     4201 atgttctttt tcaagtattc gtaaacagca accattttac catagtcagt aattgcttct
     4261 tttaatagaa agacaatctt gtatttttct agattttcag agtaccgaat acttttttgt
     4321 atcgcaaacg cagaattatg aactaaattg atagataaaa ccttttctat gcttaagtat
     4381 ctactatcat ctttactcaa atctactacc ttccccttct catctttaac agtgttatcg
     4441 acatccagaa taattactct ggtttccaca aaactctcct tgtcattgcc tttattcttt
     4501 gtcttgcttg gaataaatga ttgtgatgaa tttagtatct ctacaaactc atctcttgta
     4561 acttctctat agttatctaa acacctatct cttacatcag ttttttcttt ttttgtaact
     4621 ggcttctcta ataatttttc tttatctaca taaattggaa tattaatcat tgatgtttcc
     4681 atttgtgttt cctccttata aaattctgtt aaagactaaa acatacagat aataaaaaca
     4741 aactctttat tactatatgt t
//
LOCUS       DAJSMI010000172         4738 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJSMI010000172 DAJSMI010000000
VERSION     DAJSMI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.176"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            9..1214
                     /locus_tag="ON430_002459"
     CDS             9..1214
                     /locus_tag="ON430_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3220345.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            1230..1874
                     /locus_tag="ON430_002460"
     CDS             1230..1874
                     /locus_tag="ON430_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220346.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            1885..2691
                     /locus_tag="ON430_002461"
     CDS             1885..2691
                     /locus_tag="ON430_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220347.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTHQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKMKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            2713..2943
                     /locus_tag="ON430_002462"
     CDS             2713..2943
                     /locus_tag="ON430_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220348.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYLAAKKYFTADNK"
     gene            3501..3779
                     /locus_tag="ON430_002463"
     CDS             3501..3779
                     /locus_tag="ON430_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220349.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHALLDYKKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIEQ"
     gene            3818..4240
                     /locus_tag="ON430_002464"
     CDS             3818..4240
                     /locus_tag="ON430_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220350.1"
                     /translation="MAKATRKHIYLYDMHIEVLDEIISTHDDISNYSEAVRHLCMMYD
                     PKTEEKISPSQIKLNAIGKEVSILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            4260..>4738
                     /locus_tag="ON430_002465"
     CDS             4260..>4738
                     /locus_tag="ON430_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220351.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTLEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEYGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTEREKIEYQRLNQAMEKYQAEPHKKTSGTLPGVFSISSDEV
                     "
BASE COUNT         1798 a          712 c          960 g         1268 t
ORIGIN      
        1 ggaaaataat ggaaaaccaa aatgaaagtt taatcaagca gtatgttaag cgtcgtgcaa
       61 agcggcgctt atttttgtgg ttgtttggta cttctgcagg tttgattact attttgatca
      121 ctgtgttcgt gaccctattt ctaatacttg cggcgggctc gattgataat tcggattcag
      181 attcaagcag tggtggcgaa gcttttacag gagaatattc ggaaggctta ccgatttata
      241 aagaaatcaa aggaagaggc cctttttctg atgagattgc gcaatatgcc gtaggtgcag
      301 cagtgaaata taaattatta ccaagtgtga tcctttcgca atacggttac gaatcagcgt
      361 ttggtacatc ggcgtccgca agaaatgatc tcaactattt tgggattact tggtttgatg
      421 gttgtctgtt tcctaaagga actgcacgag gaattggtgg cattgaaggt ggctggtaca
      481 tgaagtttcc caactcaaaa gctgcatttt cctattacgg ctttatggta gccacacaat
      541 caaattttaa tgcttgtgta ggaaataaaa gccctggcgc aagcttattg atcttaggac
      601 gaggcggtta tgccgctgca ggaattactg aagatagtcc gtattataca ggttgtatgt
      661 caattatcac ttctaataaa ttaaccgaat atgatgaatt cgccatcaaa cattggggtg
      721 aaggtggcaa taacaatggc acaattactg gtgaatggac gaatccattc cctggcagca
      781 gtttagataa aagttccttc agcggtggtc aattatttgg aacaaatcca ggcggtgaat
      841 tccgtccgaa cggcttccat gatggattag attttggttc agttgatcat ccaggaagtg
      901 agattcatgc ggttcatggc ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt
      961 tgggcgcgtg tgtgatcgtg attaattacg atggcttgaa tatggtgtat caagaatttg
     1021 caaatagtac aggaaattct cgagtgaaag taggcgatca agtgaaagtt ggtcaagtaa
     1081 ttgggatacg agatacagca cacttgcatt taggatttac tcgaatggat tggcgtcaag
     1141 cacaaggaca tgcattcatt gatgatggta catggatcga tccgttacca tttttgaata
     1201 gcagtaaaaa atagatcagg agaaaaaata tggaaaataa aacgttaaaa atcgtcttaa
     1261 cagctgcaat tagcgttagt ttaatcggca atattgcgtt gttttggcag ctgaaagagg
     1321 aacaagcagc aaatacctat aaaattgaag aagtggctaa aaacgcgtct gagaagctca
     1381 aaaaaaattc ctctgaggca caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga
     1441 aaaaccaaga aatcgatcag ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa
     1501 ctgaagcaca aagaaagacg gcagaagagt tcggtcaatt ggccattgat aaatcattaa
     1561 gcagaaatga attaacggaa aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat
     1621 taagtcctac agacgatgat caccaacatg atgattacgg aagttactcc tttacattag
     1681 gtgacgtgca aacttacgct caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat
     1741 ttgttgatta tacaatcggc aatccgcaat tcaaagatgt caaaccacaa aaaataaaag
     1801 gtggattaac tgtcactgaa aaacaagtag atggtcaatg gaaagtcata gactttagtt
     1861 atttcactcg ttaggaggaa gaaaatgagt aagaaaaaag gagaaagaca acgtttcaaa
     1921 aaacgaaaaa gttatcttca atggctggga atctttatga ttctcggctt tttgattctt
     1981 gctagtccga tttacacgca tcagaaggta gacataccag atgaagtaag cacgaacgca
     2041 tttttaaaca cgaaggaact aacgatggtt tcaaaagaaa aaggagcgaa ccagcaatta
     2101 gtttttgaat ttgcaatcga cgatgaagat caaagcttat taaaagaact ggctaatctt
     2161 aattatcgcg tagaagtaaa gacagctaaa ggggattatc aagcaataaa agccaaagtg
     2221 attaaggtgt ctgatgatta ttttgcagtg aagctatcgc atgtaccaga agaatatatt
     2281 gctatgcgat taaccattat tcctgaaaaa attgatccaa aggtggatat gcaagaacca
     2341 caagatctga tttattacgt tcacgaagat aaggttaaag atcaagtgaa agatgaagat
     2401 tatgaacaac atgcgatcaa ttacaaaatg aaaggataca agaaagaaca aaaagcagct
     2461 caaaaacaaa tggaatcttt ccaagcaacc attgatttga atgaagaatt agtgaagaag
     2521 ctaaaagatc aattgcctta tcaagtcgca gatgatcagg aaaatactga aaacaagatt
     2581 aatagctacc agcaagaaat tgaaacatcg aaaacgcaaa tgaaagatca agaagagatc
     2641 attcaaaaac ttcaagaaaa aattgatcgt atgacaaaag aaaagattta gaaggacagg
     2701 tggagtgaaa aaatgaagcg aacaaacttg gctgacaaag atactttcgc tgagcagaaa
     2761 gtattgatta aagcctatct caaagttgta aaagctaaag aaatatgtcg agaagtgaat
     2821 ttatctaata caacaatatc tgagttgaag aatggtcgaa agaatttaga tactttaaaa
     2881 ttcagatcat ttgaagcttt gtatttagct gctaaaaaat attttacagc tgataacaaa
     2941 taaaaaacag gagtatttta tgtgaaaaaa gtatataaaa aatgcttatg aatcagctat
     3001 atcaccttaa ccatttgtta atgagatggg agaaaacatt aaccacgatt ctaacgcaaa
     3061 catcaagcac ataaaacatt taacactcct cctttctggc tgaagatttc aaccaaacag
     3121 gaagacagcg tatcaattat agcatacact ttttcttaaa ttcatttttc atatgacaaa
     3181 atatttcctt ctaatcgaca aagttagaag gattttttat tgtctaaata cacaaataac
     3241 cttaatttat aaagagcata aggaaacgtg tacccacttt caaaataaag tgggtagata
     3301 ggctgtgcct atcacgtttc tgaaaaaaat aaatgacccc aaaattttca aattatgtgt
     3361 aggtttatgg gtagcaaaaa tatgaatgtg ggtagcacaa aagttcattt gtgggtagta
     3421 aaatttaaat atgtgtagca atgtgtgtag ctacacacat tgctacacac gttataacat
     3481 atagtcaaaa ggagatattt atggaagtaa aaataagaga tattgatgaa aaaacgattc
     3541 aaagaattga ccgaagatgt attgaattaa gtgaacgtac tgggactaaa tggagccgaa
     3601 atcgatacct aaaagttttg atcgaaaatg attttgatca cgctcttctg gattataaaa
     3661 aagatcaatt tgatcggttg ttagaaaaat ttgtggatat tcaaacttat aatacgaaaa
     3721 ttttagaaga atatattgca acaaataatc aattaatcgc cttgttaatt gaacagtaaa
     3781 aataatattg tgaatttata aaaggaggaa atcaactttg gcgaaagcaa caagaaaaca
     3841 tatttatctt tatgatatgc atatcgaagt acttgatgag ataatttcta ctcatgacga
     3901 tatatccaat tatagtgaag cagttagaca tttgtgtatg atgtatgatc ccaaaactga
     3961 agaaaagata agtcccagtc aaatcaaact aaatgcaatt ggaaaagaag tttctatttt
     4021 aactgaaatg gtttcggagt ttgcagatct tcatttaaaa gatacaggaa tattaattgg
     4081 tacaaattca actgtttatc aagaagcgaa agagcgtgtg gaaggcaaga taaaagcaaa
     4141 tcaaacgaag aaatattctc ctaagataaa gaaaacaata agcgaatcag tgactacaaa
     4201 aagtgaagta tttgatccaa caaggttttt aaaacgataa aaattagaag gagaacgtga
     4261 tgagtaaatc acctgcagtc attctcactt gtcaatttac cttgcccaat aaaaaatctt
     4321 tttcaacata cattgattat atgacaagag aaaaagcgct ggaagaaatg gaacggcgca
     4381 cgctcgaaga agaacaggaa ctgcaacgaa tcaagaatgc tttagaggat ttttatattc
     4441 ctgaaggaga aactttttcg gagagtaaga cggataaaga attcagtgaa acgtcgaatg
     4501 aagcgcaatc gttgatggaa tatggaatca attttgacaa gttagaggaa caagatttta
     4561 ccaaatatct ttcttatatg acgcgtcact atgcgttaga acaaaaaaag gaactaacgg
     4621 aaagggaaaa aatagaatat caacgtttga atcaagcaat ggaaaaatat caagcagaac
     4681 cacacaaaaa aacttcagga actcttcctg gagttttttc tatatcttca gacgaagt
//
LOCUS       DAJSMI010000173         4729 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJSMI010000173 DAJSMI010000000
VERSION     DAJSMI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.177"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(78..1886)
                     /gene="pepF"
                     /locus_tag="ON430_002466"
     CDS             complement(78..1886)
                     /gene="pepF"
                     /locus_tag="ON430_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCR3220352.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2062..3060)
                     /locus_tag="ON430_002467"
     CDS             complement(2062..3060)
                     /locus_tag="ON430_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCR3220353.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3286..3939)
                     /locus_tag="ON430_002468"
     CDS             complement(3286..3939)
                     /locus_tag="ON430_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCR3220354.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4271..4669)
                     /gene="spxA"
                     /locus_tag="ON430_002469"
     CDS             complement(4271..4669)
                     /gene="spxA"
                     /locus_tag="ON430_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR3220355.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1386 a          995 c          742 g         1606 t
ORIGIN      
        1 aaaatacgtc gactccctta caagagagcc gacgtatttt tttcgtttct attcgagaaa
       61 atcaaaaata gaaagcctta taaacgatcg atcaattctt ctaattcatt tagacgttgt
      121 tcaaacatcg acattgcatc ttcaatatat gcagcctgtg tcatatcaac tcctgctttt
      181 ttcattactt ccacaggata atcgctgtta cctgctttta ggtaagccaa gtagttctct
      241 agtgcctctg gttcctggtt tagtattttc ttcgccagtg ctgaagcagc agaaaagcca
      301 gtcgagtatt ggaaaacata gtaattataa taaaaatgcg gtatccgtga ccattcgaat
      361 ttaatttctg ggtcttcttc gactgctgga ccataatatt ttgcattcaa tttaccataa
      421 ctatcactta ggtattcact agtcaatggc acaccttttt catcttccgt atgcatgaaa
      481 tgctcgaatt cagcgaattg tgtctggcgg aaaactgttc ctttaaaccc atccaagtaa
      541 tggttgagta cataagcccg tacacgaggg tctttttctg tttccaataa atactccgtt
      601 aggatatttt cattcgttgt cgaagcaatt tctgctaaaa agatggagta gtcgccgtac
      661 acataaggct ggttcgaacg agtgaaataa ctatgaacac tatgtcccat ttcgtggaca
      721 agcgtaaata gctgatccag cgtatcatgc caattcaata aaatatatgg atttgtgtca
      781 tagcttcccg aagaataagc accgcttcgt ttccctttat tttcgacaac atcgatccaa
      841 cgttcagaga atgctttttc tacgatggcc atgtattctt cacccattgg tttcagtgct
      901 tctaaagctt tttcttttgc ttcttcgtac gtaaaggtaa ttggagcttc ccctaagacc
      961 ggtgtataaa gatcatacat gtgcagtttt tccacttcta ataaacgttt ccgtaattcc
     1021 atgtatcgat gtaacaaagg caaatgcttg tttaccacgt ctaccaaagt atcgtataca
     1081 ctttcaggaa tatgattatt gctcaaagac gcttctctgg cagagctgta attacggact
     1141 ttcgctttaa aattatgtcc ttttatatgt gtgcctaaag tagaagcaaa tgtatttcta
     1201 aattgttcgt aaacactgta caacccctta aatgctgctt cgcgcaccct tcgatccgtg
     1261 ctttctagca actgaccata cacgccatga gataattgga ctatttcacc attttctcct
     1321 tcaatcgttg gaaaaactag atctgcatta ttcaaaacag caaatgtatc acttgaagca
     1381 tcaaagattt cacctgctcc agcaagaaga gattcctgct cagcagataa gacatgggct
     1441 cgattatcta ctatttgctg gatataatgg cgatagactt ccaattttgg ttcttctttg
     1501 aaatattgcc aaatctggtc atctgacaat tgcaatattt ctggttcaaa ccaggaaacg
     1561 gcttcactaa ctttcgaaaa cagactgctt gctcttgcat aaagcgcttg gtaatctgta
     1621 tttccagtgt cttgatcgtt tttaaggtgc gcatatacat aaatgacttc agtttggcga
     1681 taaaccctca atacgaattc aatcgcattt aaaaattgag aagcgccttg atcaagtgtt
     1741 cctttgtgtt tttcagattg ttttaactct tctgataatt ccaaatattt ctcatcaaac
     1801 tcttggtcgc ttgaaaagat cttggtcaag tcccaagtta aattttcagg caattcttct
     1861 cgttttggca actgctttac ttccatgaaa tccactcctt ttcttccctt agctctatct
     1921 atcaaaatag atcatagtct aacatccaca tgtcacagca tctaactttt ctactcattc
     1981 atgtgtctgc ttccatcaat tttagcataa ctaaatcttg ttttacatag aaaaccgatt
     2041 cctagcctat ttttatactt actatttttt caaagaagac aagtgtatgc attcgttaaa
     2101 aaactgctga tagaccatct cttcatcaat cataggaaaa agccattccc ttttatgttc
     2161 ttttatttgg caaagccatg agttgaaatc aggaactttc tggttttcca aggcttcttc
     2221 gtacaacatt cgataaagaa aaacctgttc ttgtaaataa aagaaaaacc gacttttttg
     2281 atagatccac gatgatagat gcaaaagatg tttatgacgc aagtagcatt gttcttgtat
     2341 ggatactgta ttcttttgct tgtgaataag ttggcgtgtc agccatttct tcccatcttc
     2401 actcactttt tctatttttt tcatttggtt cattttaatt tcgttactag tgaatatttc
     2461 tttaaggtcc ctactagaaa aagtccagca gctactgcaa aaggaaatat gccctgatac
     2521 agtctctgta atatgatgat acaaataaag cttttgtttc ttgagatcag ctttccagca
     2581 atgcactcct ctttttctat tatagtagca gcaacttttt tctatcaatg agaattgcga
     2641 ttgtccaaga aaagaattac caagtatcca ccatacttgg taactgttat tcaaataatt
     2701 ttgggttctt tcaataaatc tctggtgaga cagtctagaa cattggattt ctattgctag
     2761 attcgccaac aaaatatccg gcctttgccg caacctcggc aaataggctt ccaaaaatac
     2821 tggttctact gactgtccca gccaatccat gaagcattcc tttaactgta agtgctcttt
     2881 tgattctctt tcactaaaga ccgaacagtt atctgatttt tgatgggcaa aatgagcaca
     2941 ttttttcttt ccctttttca aaatcactct ttttcgacag ccacgacaat aatatttctt
     3001 tcctttttct gcatgtttag caaaacagca cttattatat tcattcattg caagcagcat
     3061 ttttctccct ctgaacttat ttacgtctaa gaatttattt acacgaaaaa agagggcgtg
     3121 aaaaagctat tctgcatcaa gtaataagaa cagctaaaca aaaatagttc ccaatatttt
     3181 tcgtttggtt gggcttgtta ccgcagatgc aagcttttga acacatttat acgaaaaaaa
     3241 gactgcgaca caatttttgt cacagtctct tactataatg ctgatttata caaaataaaa
     3301 tttcgttcgc tcgatagcat tacgcgccat cagacattgt ccatattcat tcaatgtttc
     3361 tggtgtacga tcgctctttt cagcaaactc caaaattcga gcaatctgat tctctacatc
     3421 cgcttggttg agattttcca tccagaagtg aacgactagg taatagcgct cttcgaataa
     3481 atacaaatca gtccaagcag actctagttc gacatctgtt gcaagttgga tcacttgttc
     3541 aaaatcttca aaagcaaaag ttgcttgtct ttcctgttct tctagggcgc ttccagataa
     3601 ttcttcctcc ggatcgctgc caataatctg ctgacgaaga aagtcactca catcttctga
     3661 atcaacagta gaatcttctg attgagaaat ttcttcatcc ggaagatttt tactgataaa
     3721 cagttctaat ccgtctcctt ttggcaaaac ttggaaagtg acagcttcgc ttcctcggaa
     3781 ctcctcttcg atatcaactt cttccaaaat actataaaag aaattttcga tttctttgtg
     3841 attgccaagt aaatccaaaa atgaaacgcc ccgttcctct aaatcttcat gaccaattag
     3901 tacacggatc gtattttcat taatatgttc catttccatt ttgctacacc tcactttgcc
     3961 acaatgttta atatagattc attgtagcga aatcagctta aatgtaaaga aaaaaaatct
     4021 cgctgttaag cgaatttaat aaaaccagcg gatttttcaa cctttcaagc tatcttattt
     4081 aactataaca tttttggcag acgaatacta tatagccgac ttttcagcta attttccctc
     4141 tctatttttc gtgcaatcat catcttctac taccttttta tacatctaat caagaatact
     4201 ttttgtaaaa acaaaaaaac ttggaaaaac tatctttgta atagtctttt ccaagtttcg
     4261 tattatcctt ctataatcca gccatcaatt gtgcttgacg taattctaat tgtcgtacat
     4321 cgcgcggtag gaaccgacga atttcatctt cattaaaacc aacttgcaga cgtttatcat
     4381 caatcatgat tggacggcga agcaatccag gattgttttg cacaagttcc aataattctt
     4441 gtaacggcag atcatctaaa tccatgttca gtttttggaa tactttggaa cgtgtcgaga
     4501 tgatttcttc tgttccgtct tctgtcattt gtaaaatcgc ttttaattca gaactgttca
     4561 atggttctga aaaaatatta cgttcaacaa atggaatctg atgttcttgc aaccacgcac
     4621 gagcctttct gcaggaagta caacttgggg aagtatataa tgttaacaac gtgcaccact
     4681 ccttttcgtg ataagaatca cttttttgta acagaagatc ataataatc
//
LOCUS       DAJSMI010000174         4714 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJSMI010000174 DAJSMI010000000
VERSION     DAJSMI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.178"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            19..1137
                     /locus_tag="ON430_002470"
     CDS             19..1137
                     /locus_tag="ON430_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3220356.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1265..2641
                     /locus_tag="ON430_002471"
     CDS             1265..2641
                     /locus_tag="ON430_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR3220357.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3042..4622
                     /locus_tag="ON430_002472"
     CDS             3042..4622
                     /locus_tag="ON430_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR3220358.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
BASE COUNT         1530 a          783 c          988 g         1413 t
ORIGIN      
        1 aagaaaggag gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg
       61 cttgaattat tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc
      121 gaaccgatcg ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt
      181 tactatttat tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg
      241 gctgtcgcaa ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc
      301 atcccaagtc tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa
      361 caggtagaaa gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc
      421 aataaatatt ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt
      481 ggagtatcca atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc
      541 attacagcac cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac
      601 atccaacagt ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg
      661 aataaaacat tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc
      721 ttcacatttt taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt
      781 gcgggattga caaacctaat tccatattta ggaccttatc taggactagc acctgcattt
      841 ctagtaacag tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc
      901 gtccaacagt tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc
      961 catccattaa cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt
     1021 atcttcttag gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa
     1081 atcgtcaaag aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta
     1141 gttttagtac acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg
     1201 ctatagtgcc acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc
     1261 gattatgaat gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt
     1321 tttgacattg atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa
     1381 gaatcgaatc gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat
     1441 attgcagaat ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat
     1501 tttgtctggt gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg
     1561 cggcacagcg gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc
     1621 gattgttttt ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc
     1681 aaagttcact tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct
     1741 gctttctgct tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga
     1801 ttcaaaaatg accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa
     1861 tgaagaatta gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt
     1921 catggtccca cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt
     1981 gaatgaagtt ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa
     2041 agtagtcgga atccttcata caaagaattt actaaaagct gcccataaat tcggctttga
     2101 taatttagat atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat
     2161 tgatgatctg ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga
     2221 atatggcggc gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga
     2281 aatcgatgat gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata
     2341 tatcatccaa ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca
     2401 tatgagtgat gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga
     2461 tgaaggcgaa aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga
     2521 aggttctcga atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga
     2581 acctgaagaa gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata
     2641 attggaaact tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt
     2701 ttagtcagct gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga
     2761 atccatgcta tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc
     2821 attaagtaat aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct
     2881 tattgctgat aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc
     2941 ttttgtcaca gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag
     3001 agaaaatata aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa
     3061 agcaacaagt ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa
     3121 caacgattac agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa
     3181 aaggcaaaaa aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg
     3241 ggatttctgt tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct
     3301 tggatacacc aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg
     3361 atagtgcggt catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat
     3421 tccaagttgt aaaaaagcgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg
     3481 atggtcgtga accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt
     3541 atccgatgaa ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc
     3601 ataatcgtgt agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg
     3661 atgaagatgg actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag
     3721 tcttagaaga agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg
     3781 ctcgtggcga tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga
     3841 cttttttaga gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg
     3901 atcagatggt aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa
     3961 atatgaatcc agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg
     4021 aacgaggaat ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca
     4081 cacaatttat ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg
     4141 gtgtatatga tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag
     4201 ttgcttatga agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa
     4261 atgtcatgaa acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga
     4321 ttcagcttta taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac
     4381 aattcgaagt attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc
     4441 caatgggaca caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt
     4501 caagtcgcaa tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc
     4561 aatttgcaga acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt
     4621 aaagaagtct tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag
     4681 cctctcatat tttgtttaga gccgtgacgg attt
//
LOCUS       DAJSMI010000175         4673 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJSMI010000175 DAJSMI010000000
VERSION     DAJSMI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.179"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            132..1337
                     /locus_tag="ON430_002473"
     CDS             132..1337
                     /locus_tag="ON430_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3220359.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKNSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            1353..1997
                     /locus_tag="ON430_002474"
     CDS             1353..1997
                     /locus_tag="ON430_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220360.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNLVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2008..2814
                     /locus_tag="ON430_002475"
     CDS             2008..2814
                     /locus_tag="ON430_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220361.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEISTNAFVNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            2837..3067
                     /locus_tag="ON430_002476"
     CDS             2837..3067
                     /locus_tag="ON430_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220362.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYLAAKKYFTAERK"
     gene            3331..4203
                     /locus_tag="ON430_002477"
     CDS             3331..4203
                     /locus_tag="ON430_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220363.1"
                     /translation="MKSTDWIKHTWLPLKRNSKRKEFWILQLWPLGKLCSYLARFILD
                     LIIYSIVVFGTAYYLLNDPNRIAASDIFTVLMTLIFVMLLSIFTQIVMQFTRQKLTAG
                     KLFKSLFDLIIVNAILATLIKQIEQFGRTTKGVKEFLMTFVHFVNGHMYLFEVGIILF
                     LVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYYVDFGRYDELINTDIVHTSIHEATVKG
                     IRTTLKDGQETKAIDRRKEVYNFYEVEANLRYGIGKLSLNGRNIPQNKPYIGWKSDAL
                     EVTKKLPMEEEEIQ"
BASE COUNT         1687 a          725 c          949 g         1312 t
ORIGIN      
        1 aactcagaat tagaatccat tcctcaacaa aatcaaggag acgctgtgat caattttggc
       61 tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac gtcaattaaa attgtttgat
      121 ggaggaaaat aatggaaaac caaaatgaaa gtttaatcaa gcagtatgtt aagcgtcgtg
      181 caaagcgacg cttatttttg tggttgtttg gtacttctgc aggtttgatt actattttga
      241 ttactgtgtt cgtgaccctt tttctaatac ttgcggcggg ttcgattgat aattctgatt
      301 cagattcaag tagtggtggc gaagctttta caggagaata ttcggaaggc ttaccgattt
      361 ataaagaaat caaaggaaga ggcccttttt ctgatgagat tgcgcaatat gctgtaggtg
      421 cagcagtgaa atataaatta ttaccaagtg tgatcctttc gcaatacggt tatgaatcag
      481 cgtttggtac gtcggcgtcc gcaagaaatg atctcaacta ttttgggatt acttggtttg
      541 atggttgtct gtttcctaaa ggaactgcac gaggaattgg tggcattgaa ggtggctggt
      601 acatgaagtt tcccaactca aaagctgcct tctcatatta cggctttatg gtagccacac
      661 aatccaattt taatgcatgt gtaggaaata aaagccctgg cgcaagttta ttgatcttag
      721 gacgaggcgg ttatgccgct gcaggaatta cggaagatag tccgtattat acaggatgta
      781 tgtccattat cacttctaat aaattaaccg aatatgatga attcgccatc aaacattggg
      841 gtgaaggtgg caataacaac ggcacgatca ctggtgaatg gacgaatcca ttccctggca
      901 gcagtttaga taaaaattcc ttcagtggtg gtcaattatt tggaacaaat ccaggcggtg
      961 aattccgtcc gaatggcttc catgatggat tagactttgg ttcagtcgat catccaggaa
     1021 gtgaaattca cgcggtgcat ggaggaaaag tcgtgtacgt tggcaatcct ggtatttcag
     1081 gtttaggtgc atgtgtgatc gtgattaatt acgacggttt gaatatggta tatcaagaat
     1141 ttgcgaatag tacaggaaat tctcgtgtga aggttggcga tcaagtgaaa gttggtcaag
     1201 taatcgggat acgagataca gcacacttgc atttaggttt cactcgaatg gattggcgtc
     1261 aagcacaagg acacgcattc acagacgatg gtacatggat tgatccatta ccatttttga
     1321 atagcagcaa aaaatagatc aggagaaaaa atatggaaaa taaaacttta aaaatcgtct
     1381 taacagctgc aattagcgtt agtttaatcg gcaatatcgc gttgttttgg cagctgaaag
     1441 aagaacaaga gacaaatacc tttaaaattg aagaagtggc taaaaacgcg tctgagaagc
     1501 tcaaaaaaaa ttcctctgag gcacaggcga agatcgacaa gttaaatcaa accatcaatg
     1561 agaaaaacca agaaatcgat cagctgaagc aacaagatgg cacaaaagca aaagaggaag
     1621 taactgaagc acaaagaaag acggcagaag agttcggtca attggccatt gataaatcat
     1681 taagcagaaa tgaattaacg gaaaaactaa aagatgtcgc aacccaagaa gtgatcgaca
     1741 aattaagtcc tgcagacgat gatcaccaac atgatgatta cggaagttac tcctttacat
     1801 taggtgacgt gcaaacttac gctcaaacaa gcagtgtaaa agaggaacaa aatcttgtcg
     1861 tatttgttga ttatacaatc ggcaatcctc aattcaaaga tgtcaaaccg caaaaaataa
     1921 aaggtggatt aactgtcact gaaaaacaag tagatggtca atggaaagtc acagacttta
     1981 gttatttcac tcgttaggag gaagaaaatg agtaagaaaa aaggagcaag acaacgtttc
     2041 aaaaaacgaa aaagttatct tagatggctg gggatcttta tgattctcgg ctttttgatt
     2101 cttgctagtc caatttatac gcataagaag gtagacatac cagatgagat aagcacgaac
     2161 gcatttgtaa acacgaagga actgacgatg gtttcaaaag aaaaaggagc gaaccagcaa
     2221 ttagtttttg aatttgcaat cgacgatgaa gatcaaagct tattaaaaga actggctaat
     2281 cttaattatc gcgtagaagt aaagacagct aaaggggatt atcaagcaat aaaagccaaa
     2341 gtgatcaaag tgtctgatga ttattttgca gtgaagctat ctcatgtacc agaagaatat
     2401 attgccatgc gtttaaccat tattcctgaa aaaattgatc caaaagtgga tatgcaagaa
     2461 ccgcaagatc tgatttatta cgttcacgaa gataaggtta aagatcaagt gaaagatgaa
     2521 gattatgaac aacatgcgat caattacaaa gtgaaaggat acaaaaaaga acaaaaagca
     2581 gcacaaaaac agattgaatc cttccaagca accattgatt tgaatgaaga attagtgaag
     2641 aaactaaaag atcaattgcc ttatcaagtt gcagacgatc aggaaaatac tgaaaataag
     2701 attaatagtt accagcaaga aattgaaaca tcgaaaacgc aaatgaaaga tcaagaagag
     2761 atcattcaaa aacttcaaga aaaaattgat cgtatgacaa aagaaaatat ttagaagaat
     2821 aggtggagta aaagaaatga agcgagcgaa cttggtcggc aaagatactt tcgctgagca
     2881 gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat gtcaagaagt
     2941 gaatttatct aacacaacaa tatctgagtt gaagaatggt agaaagagtt tagatacctt
     3001 aaaattcaga tcatttgaag ctttgtattt agctgctaaa aaatatttta cggctgaaag
     3061 aaaataaaaa caggaggatt ttatgtgaaa aaagtgtatc aaaaatgttt atggatcagc
     3121 tatatcaccg taggtatcgt tggcttcttt tttattgtag ccgtgacctt gggaacgatt
     3181 tttctatggt ttactaatga agatttatta ttgaagggac tcatgagtgt aatctcatgc
     3241 gtcctattga ttttgttggg gacagaaatc aacaaacttc tgccttctcg ttggcaagaa
     3301 aagaagaaag aaatgagaga agaaattgaa ataaaatcga ccgactggat caaacacact
     3361 tggcttcctt tgaaaagaaa tagcaaacgt aaagaatttt ggattctaca attatggcca
     3421 ttaggtaaac tttgtagcta tttggctcgt tttatacttg atttaatcat ttactcgatt
     3481 gtagtatttg gaacggctta ttacttatta aatgatccca ataggattgc agcatcagac
     3541 atttttacag tgctaatgac gttgattttt gtcatgctat tgtctatttt tactcaaata
     3601 gtcatgcaat ttaccaggca aaaattaaca gcaggaaaac ttttcaaaag tctattcgat
     3661 ttaattattg taaatgcaat tctagcaact ttaataaaac aaatcgaaca atttggaaga
     3721 acaacaaaag gagtgaagga atttttaatg acgtttgttc actttgtaaa tgggcacatg
     3781 tatttgtttg aagtgggtat tattctcttt ttagtcgggt atgtatccac gaaacttatt
     3841 aaacgttcaa ttgaaaaaga aattgagatt gacttgattc cgacacttct tggttatgag
     3901 tactatgttg attttggaag atatgacgaa ttgatcaata cagatattgt tcacacgagt
     3961 atccatgaag ctaccgttaa gggcatcaga actacgttaa aagatggcca ggagacgaaa
     4021 gcaatcgacc gaagaaaaga ggtctacaat ttttatgaag tcgaagcaaa tctaagatat
     4081 ggcattggaa aattgtcgtt aaatggaaga aatattccac aaaacaaacc atacattggc
     4141 tggaaaagtg atgctttaga agtaactaaa aagcttccta tggaagaaga ggagattcaa
     4201 tgagtctagt caatttgaat catttgtttg aagatatcag taatcatttc caacagttct
     4261 caataagagt ttatattatt cattagttta ttttgattga aaaaagaaga aaaaatgtag
     4321 aaagttgtct gtgaaaaaag tgtatgctat actgataatg caaacacata aaacatttaa
     4381 tactcaaaaa gagccctagc agttgcagct gcgtaaggct cttttcttta ttcactatta
     4441 accaaataat gaacaggcta gctgtcttta tcatgtttag aatctaacca tttgttaatg
     4501 agatgggaga aaacattaac cacgattcta acgcaaacat caagcacata aaacatttaa
     4561 cactcctcct ttctggctga agatttcaac caaacaggaa gacagcgtat caattatagc
     4621 atacactttt tcttaaattc atttttcata tgacaaaata tttccttcta atc
//
LOCUS       DAJSMI010000176         4527 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJSMI010000176 DAJSMI010000000
VERSION     DAJSMI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.180"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..65)
                     /locus_tag="ON430_002478"
     CDS             complement(<1..65)
                     /locus_tag="ON430_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HCR3220364.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYA"
     gene            457..1479
                     /locus_tag="ON430_002479"
     CDS             457..1479
                     /locus_tag="ON430_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR3220365.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            complement(1604..2038)
                     /locus_tag="ON430_002480"
     CDS             complement(1604..2038)
                     /locus_tag="ON430_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3220366.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            2153..3127
                     /locus_tag="ON430_002481"
     CDS             2153..3127
                     /locus_tag="ON430_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3220367.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            complement(3525..4478)
                     /locus_tag="ON430_002482"
     CDS             complement(3525..4478)
                     /locus_tag="ON430_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HCR3220368.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
BASE COUNT         1445 a          811 c          892 g         1379 t
ORIGIN      
        1 gcgtagcctt ctgctatttg actgcggcat gaattgcccg tacataagaa atagattttt
       61 ttcatttgat ttcctccgtt atggattagt aaatgtcact gtttatctaa agtaaactct
      121 atgtgatgga gcgaaaaaac aatgggtttc ttagtttata tatttctttt tgttcagcgt
      181 ataaatcgat gtgtacaaag aactaatctt gtctttttta ccataaatac atgttagaaa
      241 caatcatttt gagtcaaaaa tcaacgatgt aacagactgt taaaagagat taagacacgg
      301 cagatgatga agaaagtagg ggtgtagtaa gccgatacat ttcttgattg tgaggcgaga
      361 acaattattc gtcttttaag aaaaaaagta gtagaatcag ttcatattaa aaataaaaaa
      421 ccttccattt ttgtaggttt aaaggaggtc attaccttga aaaaaatcgg ctttttaagc
      481 tttggacatt ggatggagca aggatcgctt gtgaaatcag ctcaagatgc ttatttgcaa
      541 tcgattgatt tagcggtgga agcagaaaaa atcggagtgg acggagcgtt tttccgcgta
      601 catcattttg cccctcagat tgggtcgccg tttcctttgc tagcggcaat cggcgcaaag
      661 acaagtaaga ttgaaatcgg tacaggactt attgatatgc gatatgaaaa cccttattat
      721 atggcagaaa atgccggatt gacagatatc atcacgcaag gaagagtgca actaggagtg
      781 gggcgtggtt cacctgaaca agttctagat ggctggaaat actttggtta tgattacagt
      841 gaagaagaaa tcaaagaggt aacacgggaa cgcacccttg aattcttgga attacttgaa
      901 ggaaaaccat ttgaagaacc aaatcctcaa ccaatgttcc cacaaggatc aggaaaatta
      961 cggatagaac cttattcaga aggattacga caaagaatct ggtggggagc tggttcgcag
     1021 caaacggcaa tctgggcagc acaacatgga atgaatctgc aatcatctac attagtcaat
     1081 tacgaatccc acgagccatt ccatatccaa caagcaaacc aattacgtgc gtataaagaa
     1141 gcgtggaagc aagcaggtca tgattttgaa ccaagaacat tagttactcg ctcgattcag
     1201 ccaatcacca ctgatttgga tcgacgctta tttggacaag acggtagtcc gcaacaagat
     1261 gctgtcggct atcttgctta tcaccgaaac ccaacgattt ttggaaaaac ttttgctggt
     1321 gaacctgatc aattaatcaa ggagctagct gaagatgaag caatcaaaga agcagacaca
     1381 gtacttgtca cgattcctaa tactttgggg gtagaatata acatgcactt gcttgaaaca
     1441 atcgtaaaag atatcgctcc agaattagga tggcggtaaa aaggatattt ttgtaagtgc
     1501 aggatacttg agtgaaaaaa taaacacttc taacaatcag ttagaggtgt ttattcatgt
     1561 tttcatgaag ttcatagaga aggagagtga gttatccgtt tgttcaatga gaatgtacat
     1621 aagcttcttt tacatgttgg ggtaaatcat caggaatcat tgtcaatacg gcttcttcat
     1681 tgccggcttt tattgcttct tcatagtacc agcttttgta gtttagcata gcgagtactc
     1741 tttctaattt tgctatttct ttttctacta cttctttttg tttttcaaat agttctttac
     1801 gaacctcaaa tgtcttactg ccttctttgc tccattccat gaattgctta atgtctttta
     1861 tttctaaacc agattttttc aaacagtcaa tgactcttaa agcctctaac tcattctctg
     1921 aaaattttct aataccagaa acacgctgga tatcaggaaa taatccctct ttgtcgtaat
     1981 aacgtaaagt agaaacaggc aatccaaaca tttcagacac ttgtccaatg gaatacataa
     2041 aaaaatcctc ctaaaataaa atttttacct tgacctaaag tcaagtttaa gttttatcat
     2101 aataatacat tcaaaaacaa atgtcaattt tgatcaaagg agagtctaga atatgaataa
     2161 agaattaaac atcacacaag aatgggacaa agtatttcca aaaagcaatc aagtcaatca
     2221 tcgaaaggtg agcttcccga atcgttttgg tatcacatta gtcgctgatt tatatgaacc
     2281 aaaagaagta agtgaaaaga aattgccggc aatcgctgtg agcgggccat ttggtgcagt
     2341 gaaggaacaa tcgtctggtc tatatgcgca gacgatggct gaaagaggct ttttaacgat
     2401 tgcgtttgat ccatcgttta caggagaaag cggaggagta cctcgatatg ttgcttcgcc
     2461 tgatatcaat acggaagatt tccaagcggc agtagacttt ttatctgtac aaaaaaatgt
     2521 aaaccctgag agaatcggaa tcattggtat ttgtggatgg ggaggtatgg cattgaatgc
     2581 agcagctatc gatacgcggg tcaaagcaac agttgtttcc acaatgtatg acatgactcg
     2641 agtaacagca aatggctact tcgatgttat ggatgaaaat gcccgatatg aaatgcgtga
     2701 agaattaaat gcacaacgca tcattgattt caaacaaggg gactacgcac gtgctggagg
     2761 tgtggttgat ccgttgcctg aagatgctcc tcaatttgtc aaagactatc atgcgtatta
     2821 taaaacagac cgcggatatc atgtccgttc tttaaactcc aatgatggtt ggaataggac
     2881 atcagggttg tcacttatga atgcaaaact attgcattat attccagaaa tccgtcatgc
     2941 agtcttagtc attcacgggg aaaaagctca ttcacgttac tttggggagg acgctttcaa
     3001 agaactaaaa ggtgacaaca aggaactcat gatcattcca ggagcatccc atacagatct
     3061 atacgaccag acagacatca ttccatttga aaaaatggca acattctttg aagagaacct
     3121 tgtgtaacca aaaggattta atctaaaaga gattatgaaa aaactgccct gcatcaagta
     3181 ataaggacag ccaaaaaagc tcctcatttt tgacttggtt ggactcatta gcgtagatgc
     3241 aagcttttga acaccgttta taccaagagg tcgtgagcct gccgtttaac tctgagtcac
     3301 aaggagcaat ttcaaaaaat agcttctcat gtttgttgaa tattgtgact tgtgaccgag
     3361 gagttggcag gagaacaccg tttattcgaa aaaaggactg tgacaatgta tcgactccca
     3421 aaagttagac caaaaaatct aacttttggg ggtcggtaca cacgtttttt gtcacagtct
     3481 ctttgtgtct catttcatat aaacaattat ttactttgta ctatttattt catttctgtg
     3541 attttaatgc tttttgctaa attttttaca tcagctggaa taactttccc gacttctgat
     3601 tgctgtgcat tcgacatgcc gcaagccatt gcgtacctta cacattcttt taaaggataa
     3661 ttctgttcta atgaatgagc aaaacctcca acagtggcat ccccagagcc tgttgtatta
     3721 atgataggaa tcttagggat ctcaacattc caaaattctt cttttacttt gactactgca
     3781 ccatacgagc cgcaagaaat catgataaca tcgatatctt taaatagtgg atttgttttt
     3841 agatggtgtt ttatgtcgct tttttgagta attttttgat ggatgatatc tgaaaactca
     3901 tggatattag gtttgatgaa catcggttta tacttacttg ctaagacttc acggagatgt
     3961 tgtccagata tatccattaa tatataggtg gaaggatcta tttgttcttt gatatattgt
     4021 aataatttag catacaaaaa gggatctgga ttgttaacag aaccatttat tgtaatgact
     4081 gaaaggtaat tatttttact gattagtttg atatcattca ttaatttttt gatagtagta
     4141 tctgctattt caggaccagc ttccactagc tctgtctgcg tttcatcatc atgcatgatc
     4201 gtgacggcac ctcttgattc ttctgcaata ggtaaaaaat gcaaatcaaa caaattttca
     4261 tttttcagca tatcttgaat catactgcca aaataaccgc ccaaaaaccc tgttaaaata
     4321 actgatgaac ctagtagagc agaagctcgt ccgcaattga ttcccttgcc gcctacatag
     4381 cgtaccggag aatcaaagcg tgtcatcgta cctaattcaa agtgttttgt aaaataaatg
     4441 gaatcaatgg atgggttcat cgtgattgtc agtatcattt ttgggctcct ttaatattgt
     4501 taagtatttt gaataatgat tgaaatc
//
LOCUS       DAJSMI010000177         4404 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJSMI010000177 DAJSMI010000000
VERSION     DAJSMI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.181"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(106..1053)
                     /locus_tag="ON430_002483"
     CDS             complement(106..1053)
                     /locus_tag="ON430_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3220369.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="ON430_002484"
     CDS             1299..1763
                     /locus_tag="ON430_002484"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR3220370.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="ON430_002485"
     CDS             complement(1825..2868)
                     /locus_tag="ON430_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCR3220371.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(3245..4210)
                     /locus_tag="ON430_002486"
     CDS             complement(3245..4210)
                     /locus_tag="ON430_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCR3220372.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         1507 a          827 c          838 g         1232 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gcttctacgg aaataagccg aaatttcaca aaaatttgag aagcaatttt
     3061 cgtaaattcc ttcttatttc ttgaagctga ccacttcttt tacgacctct tggtaaacgg
     3121 tgttcaaaag cttgcatctg cggtaataag cccaaccagt acaaaaacat gaacagctgt
     3181 ttttgtttgg ccgttcttat tacttgatgc agaacagctt tttcacaacc tattgaatcg
     3241 attattaagc gattttttta gaaacaaatt ttccatgttt gctatgttct ttaattaaga
     3301 aacgtacgta atgagcaaga acgtagaaga caactacact tgcaaacaga cattccgcaa
     3361 cttttaagaa atccaatacg ctactataga tacctaaact gttgaagtaa tgaacagtag
     3421 taaatatagc tgttgcagag ataacaagcg ggaatgtaaa ggctgcatag cttggataaa
     3481 aacgaagctt gaacaaacgt ggaagcatag acagcacaac gaaatacaat acttgagata
     3541 aagcgaacaa tgctacgaca agataaaaat taggttcagc aaagttttta agataacctg
     3601 ttaggcaaag agaaccaggt gctgcaacga tcgtaatcag cggacgtgtc ggatgagcca
     3661 tatttcttac gaaaaagaca cgtttgatga tgatcggcaa taagaacgca tagcagatca
     3721 atgaaatcca aaagaaaatt tttccaataa gtgggaagaa gttcccggca gttacagtga
     3781 tgaccccaaa tccaacgtaa atgataaacc agcttggata aattgcttca attgaaagat
     3841 cagctttgat aacatggtga taattgaaat aaaagacaag gatcacttgg aagataacag
     3901 cgcacagcca aatgaatttg acaattggtg caatactttc atatgaaaca aaataagtac
     3961 aaataaccat gagtgacatc gtaaaagttg gtgaaacgga ggcgatgatt ggatcatgca
     4021 acgtttgttt cgtgtgttcg aataagataa acaatttaac aaaaatgaat atcataagaa
     4081 tcatagagat accgccagtt agattgccta aagcaacatg gtgatagtct ttaaacagat
     4141 ttccaaggga agctaaccca aggatcaacc cgcagatcgg gatcggaata attttcaaaa
     4201 agttcttcat atataatatt ctccttttta tttaatagat tttagtacca agatgaacga
     4261 atacattgta aaatgaatag gaatgtatga ttacgagcaa gcagacagct gattgtcttc
     4321 atctaagtta ctagttggat cagcaactgt tttgttgtac atttctatta tagatggttt
     4381 tttgtatatt taaatcaaat agtt
//
LOCUS       DAJSMI010000178         4394 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJSMI010000178 DAJSMI010000000
VERSION     DAJSMI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.182"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            195..1043
                     /locus_tag="ON430_002487"
     CDS             195..1043
                     /locus_tag="ON430_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR3220373.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1188..2129
                     /locus_tag="ON430_002488"
     CDS             1188..2129
                     /locus_tag="ON430_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCR3220374.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2119..4290
                     /locus_tag="ON430_002489"
                     /pseudo
     CDS             2119..4290
                     /locus_tag="ON430_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         1374 a          862 c          815 g         1343 t
ORIGIN      
        1 tactttaaaa aaacataata agatcacttt gcttgtagga tgtattccct tccattacaa
       61 tgaaataaat agcgatttta tgagaaggtg atgtgaaaat ggaagaaaaa atagaagaat
      121 ccaaacaaaa aatcgatcag ttgatataag aattagaaaa agcggtcatc ggcaaacgtt
      181 caactatcca attgatgatc gtggccctat tagcaggtgg tcatgtctta ttcgaagata
      241 ttcccggtgt aggtaaaacg ttactggtca aagcactagc aaaagctgta cagggaacct
      301 tcagcagagt ccaatgtact cctgatctgc tgcctagtga tattcttggt ttttccgtat
      361 acaattcaca atcaaaagag tttgaatttc gtccgggacc gatctttacc acgatcttgt
      421 tagcagatga gatcaatcgt acgactcctc gtactcagtc agcactttta gaagcaatgg
      481 ccgagaatca tgcaacgatc gacaacaata cataccctct agacaatcat ttttttgtct
      541 tagctacgca aaatccaatc gagtacgaag gaacatatcc tttgccggaa gcacagctcg
      601 atcgtttttt attccgacta caaatcggtt atccttcttt tgatgacgaa cttttgctac
      661 ttttggataa attcgaaaaa gtgttggata acttaaatga agtattgaac agttatgaaa
      721 tagaagaaat aaaaacaaat gtggcgaatg tctttgtcag tcccgaagtg gcatcttatg
      781 ccttgcagtt agtgtcagca acaagaagtc atgctgccat ccagcttggc atcagtccac
      841 gaggaagcct ttcctttatt caggcagcaa aagcttatgc tttaatccat gggagaaatt
      901 acgtcacgcc caaagatttg caggatttag ttccttacgt gttttcccat cgcctgattt
      961 tttacgatcg ttcattgaag gaagacgaaa agaaacaaat cattcatact cttgttgcac
     1021 aaattcctat tcctgtgagg tagattgagg tagacaatgg aaaaaatcaa agattatttc
     1081 attaattttg tgttatttct ggtatatttc atcattgtgc tttttgctct tgtattcaac
     1141 aattctttag gctggttctt gttggccttt tttactcttt atgtatcatg gagcttgatt
     1201 tcattaagca gctttctaaa gaaactttcg gttacttgta cattgcagcc actcgtttat
     1261 cgcaaaaaaa gtatgcaggt tattttttcc atagcaaaaa agagaacttt ttctttacct
     1321 ctccctcgtt tacaaatcgg cttcccagca atctttgcag aaaaaaacaa gagattttga
     1381 ttcttacaaa taaaccgcaa gaagtccaat tgctttggca gccaaaagaa agaggatgcc
     1441 atgaaacatt aactgtcatt tttacagcct atgatagtct aggacttttc agaaaaagtg
     1501 caaaaagaga gatctctttt cctgtgactg ttctgcctgc tttttgtaag attcaagcag
     1561 aaaaattaca gagagttctg gaaaaaaaac agcagctgaa cgcgtaccaa agaggtcttg
     1621 attttcggga acatcgcgct tatcgtccag gtgattcttt caaccgaatc gattggaaat
     1681 tgtctgctca tactcaagag tggttatacc gtgaatacga ttatcaagaa gaagaatgtt
     1741 ctcctctttt ttgcttttgg ggttcgccca gtccaaaatt tgaacctctg ctagacttgt
     1801 atttttcttt atggcatttg agaaaagaaa agcagccgga acttctgata ttaggcaacc
     1861 gcgcctttca tggcaaagac cccggtcata cctattttgc ttctttggaa gctggcgatg
     1921 agaaaacttt tttcgcacac ttgaaaaaat atgcaaaaaa acaaatcgtc ctctttgttc
     1981 ctgagaattc tcctgaagtc tcagaagcta ttgaaacatt gtcaaaagat cgaacggttt
     2041 atcttgttta tttcgtagaa aaacaactca tggtccaaga aaaagacaag atatgttcat
     2101 tgccaggagg tgaatggatt gacgattaaa cgggaaaaat ggctgcgtgg cgtttactcc
     2161 tttgtgatac taagtgtagg tgctccgata tttgtagtag ctaatcaatt gcaaaacagc
     2221 taccttctta tcctaactat tggtgtccta tgccttattg tctgctgtat tcctaaaaaa
     2281 tgggtacgtt tgctgctttc tttgccagta atgataggtt gtttgttttt atattttcct
     2341 tcgaagacat tttccatatt atggatctta cagttttttt cgtcagttac agaggaattc
     2401 ttccatctat cccaaggtaa actttattat ttgccagaaa agacagcttt ttttctcata
     2461 atgttgctga tctatttatt tattacattg accgtggatt acgactattg gtacgcaccc
     2521 tttctttctt tgatggctta cttcatgatg cttaccgttt ttcggaaaaa agatttactg
     2581 tacgaaatga ttgcggtagt aactgttttg ttcgtgtatc tggcagcaag gcaatttttc
     2641 tccatccggt tgttgtcgag aaacactcga ttccagtcat tgactacagt tctgcttatt
     2701 tgtttcttaa cttttagcgc tatcttgcct ttatatcttc cgaaagtaca ccaatctctc
     2761 gaggaaacga gtgaaccgat ccgagaatat cttaatgatg aagggcttta tgacacactc
     2821 catgagtatc aattaaccgg aagtgcaaga acaggcttca gtgagaatga tgaacagctt
     2881 ggtggacctt tatatgataa tggcgaagtt gtttttacag cgaaccaaaa aggaaatggc
     2941 tattggaaga tcgaaaataa aaatcattac accggttctg gatgaataga agatcaggac
     3001 gcacccatcc aaccaatcca agaattccct gttgacatca cgagctacca aaccgttata
     3061 gaagacaaag aaaacattac attgacccta aaaaaacaag ctttcttacc ttatccttat
     3121 ggaaatacta ccttgactga tttttcatta gctagtggac aaacgcttta ctatttgcct
     3181 gaaagccagc gattcattcc tgtcccgaat gaatcgacac gttctgcaac tttacaaaca
     3241 gaaaggttag tttttacgag agaagaatta aaaagcagtc agttaaaagc tgatgattta
     3301 gaaaatcagc agacaaatct tcaattgcca aaaagtttgc ctaaacgtat tgaggacttg
     3361 gcggtcgaat tgaccaaaaa tgaagcaact ttaattcgta aagtcgaagc tgtctaacag
     3421 tatttaaaaa caagcggaaa tttccgctat tccaaaacgg ataccgggca tactccaaaa
     3481 gaacacgatt atgtggatta tttcctcttt gattcaaaag tcggctattg tgacaacttt
     3541 tcaacttcta tggtcgtcct tcttcgaaca cttggtatcc cagctcgctg gacaaaaggt
     3601 tttacaaatg ggctcagaac aggcacagat aatcaaggga atgaggaata cagcattctc
     3661 aatagtcatg ctcattcttg gccagaggta tactttcctg gatttggctg gcttccattt
     3721 gaaccaaccc cttcatttgc caatccaatg ggttcacaac cagttgctga aacaaccgaa
     3781 accgtccagc ctgctatcga acaaccaaat gaaagcgaaa atatggcaga aactatttcc
     3841 agtgagagat ctgaggtcat tccatcagaa gaaacaacac tcacaccagc ttataaaatc
     3901 gtcttattca gccttggagt gtgtttatcc gtcttgttgt tctttctatt gttattcaga
     3961 aaatctctgc gctggacaag ttatcttatc tatcttcatt tttttgcttc tactaatacg
     4021 attgcatatc aaaaacttct taaccctttg gaaacattgg taccgcgcaa acccgcagaa
     4081 tctttgcagg aatatagcct gcgaatagaa caagaattca agctagatct gcgtttcatt
     4141 cgattaacta ctcaatatga agcagaaata tatggacgac aagcagatcc ggtaaaattc
     4201 gataaaaaaa cctatttaga aataacgcat ataataagaa aaacggtata caaaaaaatt
     4261 ctgacaaaag ggagccgctt caaaaaataa gataaaattt ctggagaatc tacttctcga
     4321 atgccgagaa ccagctgatt gagtatcgat tactcgaaaa aaaggttgtg acacgttttt
     4381 tgtcacaacc tttt
//
LOCUS       DAJSMI010000179         4374 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJSMI010000179 DAJSMI010000000
VERSION     DAJSMI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.183"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(83..1051)
                     /locus_tag="ON430_002490"
     CDS             complement(83..1051)
                     /locus_tag="ON430_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3220375.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="ON430_002491"
     CDS             complement(1498..2904)
                     /locus_tag="ON430_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCR3220376.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="ON430_002492"
     CDS             complement(3126..3488)
                     /locus_tag="ON430_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220377.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3830)
                     /locus_tag="ON430_002493"
     CDS             complement(3492..3830)
                     /locus_tag="ON430_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220378.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3809..4228)
                     /locus_tag="ON430_002494"
     CDS             complement(3809..4228)
                     /locus_tag="ON430_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220379.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="ON430_002495"
     CDS             complement(4221..4361)
                     /locus_tag="ON430_002495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220380.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DAJSMI010000180         4327 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJSMI010000180 DAJSMI010000000
VERSION     DAJSMI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.184"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            23..253
                     /locus_tag="ON430_002496"
     CDS             23..253
                     /locus_tag="ON430_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCR3220381.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            350..1651
                     /gene="murA"
                     /locus_tag="ON430_002497"
     CDS             350..1651
                     /gene="murA"
                     /locus_tag="ON430_002497"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR3220382.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1664..1840
                     /locus_tag="ON430_002498"
     CDS             1664..1840
                     /locus_tag="ON430_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR3220383.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1975..2271
                     /gene="yidD"
                     /locus_tag="ON430_002499"
     CDS             1975..2271
                     /gene="yidD"
                     /locus_tag="ON430_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR3220384.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2455..2922
                     /locus_tag="ON430_002500"
     CDS             2455..2922
                     /locus_tag="ON430_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3220385.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3007..3891
                     /locus_tag="ON430_002501"
     CDS             3007..3891
                     /locus_tag="ON430_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3220386.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4005..4241
                     /locus_tag="ON430_002502"
     CDS             4005..4241
                     /locus_tag="ON430_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCR3220387.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         1488 a          661 c          956 g         1222 t
ORIGIN      
        1 aatcgttaga aaggacgtca ttatgcaagt atacggaatt gatgcggtgc ttcgaatcgt
       61 gagtcatttg gcgtttatct acttagcctt ctggtcttta cgttcgttac gcattgaaac
      121 ttttttcaga ccaatgcata atacgcaaat tagaatggca atcgtacttt tttcaatttt
      181 tttagggtac actgcaagta attttttctt agaagttatc gctttatgcc gaaatctttt
      241 cgttagcctg taaattttaa aaaaaggtac tattttaaaa gcaaggatgt tataatagaa
      301 aagattttga gtaagtgtat gctcattgga attttttgga gggaacaaca tggaagagat
      361 catcgtaaga ggtggcaatc aattaaatgg aacagtaaga attgaaggag ctaaaaatgc
      421 tgttttgcct attttagctg ctagtttgtt agcagaagaa ggtattacga cattagacaa
      481 cgttccgatt ctttctgatg tcttcactat gaatcaagtg attcgtcact taaacgtaga
      541 tgtagatttt gatgaacaaa agaatcaagt aacaatcgat gcttcaagac aattagaaat
      601 cgaagcgcct tatgagtatg ttagtcaaat gcgtgcttca atcgttgtga tgggtccgct
      661 acttgccaga aatggacatg caaaagtagc tatgcctggt ggatgtgcga ttggtaaacg
      721 accaatcgat ttgcatttaa aaggatttca agctttagga gcaaaaatta ttcaaaaaaa
      781 cggctatatc gaagcaattg cggatgaatt gatcggtaac acgatttatt tagatttccc
      841 tagtgtaggt gctactcaaa atatcatgat ggctgccgta aaagcaaaag gaacaaccat
      901 cattgaaaac gtagctcgcg aaccagaaat cgttgattta gcaaacattt tgaataaaat
      961 gggcgctcaa gtctatggag ctggtactga aacaatgcgt attgaaggcg tggatcattt
     1021 acatgcagtc aaccactcaa tcgtacaaga tcgtatcgaa gcaggtacct ttatggttgc
     1081 tgctgcaatg acacaaggca atgtgctgat tgcagatgct atctctgaac ataaccgccc
     1141 attgatttct aaattgatcg aaatgggtgc agaaatcatt gaagaagaag gcggagtccg
     1201 tgtcatcgga ccaaaacata tcttacctac agatgtaaaa acgatgcctc atcctggttt
     1261 ccctacagat atgcaagctc aaatgacagc tatccaatta gttgcagaag gtacaagtgt
     1321 agtgactgaa acagtgtttg aaaatcgttt ccaacattta gaagagatgc gccgtatgaa
     1381 cgctcatgtc aaaatcgacg gaaatgtagc aatcatggat ggaaaccatg aattgcaagg
     1441 tgcagaagta tatgcaacag acttgcgggc agcagcagca ttagttttag ctggcttgaa
     1501 agcaaatgga atcacacgtg tgcgtaactt gaattatcta gaccgtggat attataattt
     1561 ccatatcaaa ctacagcaat taggtgcaga cgttgaacgt gtagacatgg atcaaacttc
     1621 tgctgaaaaa acagcacaaa cgattgctta aggaagtggc tgaatgagta accaacgata
     1681 tatcttatta actttgataa agattttagt ggtgatctta ttgcttatcc ttctttttgt
     1741 ggccggaacg atgatcggat atggtgtcat cggtggcggc aatccattta aagtattcca
     1801 gccaagtttg tggattcata ttcgagactt ttttcattga taaataagaa tcacagtggt
     1861 tctagtcgat atgaagtgag ccatgaagca gaacatgaca aaagaaaaca gaagggaacg
     1921 ttgacgttag tcgcgatccc tttttttagt aaaagaaaag aaggaatcta attgatgaag
     1981 aagatattga tttatttagt tagaggatat cagcgcttta tttcgcctgc atttccacct
     2041 agctgccgtt attacccaac ctgttccaat tatatgataa aagcaattca aacccatggt
     2101 gcactaaaag ggacggtaat ggggactgca cggatcttac gctgtcatcc ttttgtcaaa
     2161 ggcggtatcg attatgtacc attgacattc caattaacgc gaaataagaa agataaaaaa
     2221 gaaccagatt ataaaaaggt tgtgaaaaat acgagtagca ctgagcgata aagaaacaat
     2281 ttggaaaata gcttcttata ttttgcaaaa ttgtgagtta ttgccgaagt gttggatttt
     2341 gaacaccgtc aaaaaaaggt tgtgaaaaat acgggtagca ctgagcgata aagaaaaaga
     2401 ggaaaattat tgtcaaagta acaactccgc aactacttat atgggggaca aaaaatgatc
     2461 gaagaactaa acaagaaaga tttaccatgg tccttgttat tagatgcaga tcctgataaa
     2521 gaaaaagttc aagtatatgt ttccagaggg agtggctgga tatggaaaga aaaagaaaaa
     2581 acaataggtg tactgatcta tgtggcaagg gaaaaagaat acgaaatcgt caatgtagca
     2641 gtagccccaa cgtatcaagg aaaggggatt ggcggaaaat tacttgagac agcttttcag
     2701 aagctttcac aactcgcttc cagtcaaact cagatcatca tacgaaccgg cagtactagc
     2761 agtgcggcgt tacatctcta tcaaaaaatg ggctatgtgg agattggtcg agtcaaagac
     2821 tattttatcc atcattactc tgagcccatt tttgaaaatg gagatcaatt aagggatcaa
     2881 gtaattttag caattcactt acctaaaaat agctttaact aataaacttt caattcgaga
     2941 aaatgctttc aaatgctaaa tagatacatt acaatagctt tagcaaacaa aaaggagaat
     3001 atttttatgg gaatattagt ttttgatttt ggtggatcgg ctgtcaaata tggttgttgg
     3061 gatggcaaag agattaaagg taagggacaa tttgcaacgc ctgaatcttg ggaagaaatg
     3121 aaagcgcaat tatttcaagt ttataaaaaa atactcgttt cttttgaagt cgaaggaatt
     3181 ggaatcagct ctcctggagt agtgaataat gagaaacagg tgattgaggg aatcagtgcg
     3241 attccttata ttcatggatt taatattttc cgagatttgg aaactctgtt tcaactccca
     3301 gtaacgattg aaaatgacgc caactgtgcc ggaatggctg aattttatga aggtgctgcc
     3361 cgtgattatc aagatgttgc ttttgttgtg gcaggaactg ggattggcgg ggcgttgttc
     3421 acgaaaggac aaatcaataa aggggcacat ctttatggtg gagaatttgg gttgatgttc
     3481 ttaaatggcg ataagacatt cagtaaactt ggtacagcgg tacagatggc ttggcgttat
     3541 tgtgaacgca aaggtctcga taaaaattct tataccggaa aagatgtctt tgaattagca
     3601 gaaactggag atgcaatagc aaaagaagaa gttgagagtt tttatacgta tcttacaaag
     3661 ggattattca gtattcaatt ttcgtttgat ccagaagtga tcgtattagg cggcggtgtt
     3721 tctgcaaaag aagggctcat tgatgagatc aaaagccgaa tgaagaagct gacagaacaa
     3781 ttcgatctgc atgatttcga accgcagatt ttattatgtg aatacagaaa cgatgctaat
     3841 cttgtaggtg cagcagcaaa ctatctcgct gtacgtcaag gggagaatta agagctttag
     3901 agaaggtgca gcttgcataa aagaagtgaa tgaaatgaat aggagatttt tacctaagga
     3961 atgttttatt tgctgcagta tgataaaata tctgagaggt gaaaatgatg tcaaaaaaaa
     4021 ataaagatgt tgaagtgcgt atagaagaag tgaaaaaaga tatcaaaggt aagaattatg
     4081 aagtcactca gttatttata gggaaaaaaa tgattggtga gatcttagcc tatgggccaa
     4141 aagaatatga aattttcttt ggtgaagaag attttggaaa agaaaaaagt cttgaaaatg
     4201 cgatagaaac agtgattcga cactggaatc tgcacgaata aaaaaatatc aaaaaaactt
     4261 tcaataaagg gttgcaaaaa tctggaaaat taggtattat aactaagtca ggttgataca
     4321 aaccttt
//
LOCUS       DAJSMI010000181         4278 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJSMI010000181 DAJSMI010000000
VERSION     DAJSMI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.185"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..660
                     /locus_tag="ON430_002503"
     CDS             <1..660
                     /locus_tag="ON430_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3220388.1"
                     /translation="ISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRR
                     SIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIE
                     KKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSN
                     GLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNL
                     I"
     gene            924..1706
                     /gene="rpsB"
                     /locus_tag="ON430_002504"
     CDS             924..1706
                     /gene="rpsB"
                     /locus_tag="ON430_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR3220389.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1805..2686
                     /locus_tag="ON430_002505"
     CDS             1805..2686
                     /locus_tag="ON430_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR3220390.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2822..3544
                     /locus_tag="ON430_002506"
     CDS             2822..3544
                     /locus_tag="ON430_002506"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR3220391.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            3547..4104
                     /gene="frr"
                     /locus_tag="ON430_002507"
     CDS             3547..4104
                     /gene="frr"
                     /locus_tag="ON430_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR3220392.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1559 a          722 c          913 g         1084 t
ORIGIN      
        1 attagttgtt ctgctcgtac catttatcgt atgttcaaaa aggggctatt tgattcttct
       61 gacttaccga tgaaaggcaa gcgtaaaccg aatggacatc aagaaagacg tggaaaacaa
      121 actttccgcc gctctattca tgaacgtgaa aaggattata gccaattctc aaatgagttt
      181 ggtcaccttg aaggtgacac tatcgtaggt ctgaaacata aaagtgctgt aattacctta
      241 gttgaacgat tatcaaaagt tatcatcaca ttgaaaccgt gtggtagaca agcgattgat
      301 attgaaaaaa aattaaatca atggtttgaa tctgtaccga aaaacctatt caaatccatc
      361 acttttgatt gtggaaagga attttcaaat tggaaacaga tcagtaatgt caatgatatt
      421 gccatttatt tcgctgatcc aggaacgccg tctcaaagag gcctaaacga gaattctaac
      481 ggattgttac gtagagatgg tttattgaaa tctatggatt tcaattcagt agatgaattt
      541 tttattcaat ctgtcgcatc taaacgaaat aatattccta gaaaatcact ggattatcga
      601 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
      661 caattaagag ataataaaga aacaaagaga aatctactgt ttttcttgaa ttctttttat
      721 ctttcaggta aactattgaa tgttgagcag aaaagtatat ttgctcttgc gatacggatc
      781 ttttgtctgt ataatagctg agggtgcttt atgcatcagc aaatccacat tcttattgga
      841 ttgtcggtag gtgcttgaaa aagtttgccg gcaaggtaga agataagaag aggaaaaata
      901 aaaccaaatc ggaggaaaca aacatggcag taatttctat gaaacaatta ctagaagccg
      961 gcgtacactt tggtcaccaa actcgtcgct ggaatccaaa aatgaagaaa tatatcttca
     1021 cagaaagaaa cggaatctac atcattgact tgcaaaaaac agttaaatta gtagatgcag
     1081 cttatgatta catgaagagc gttgctgaag aaggcggcgt agcattattc gtaggtacaa
     1141 aaaaacaagc acaagaagca atcaaagaag aagcaactcg tgcaggacaa tacttcgtta
     1201 accatcgttg gttaggcgga acattgacaa actgggatac tatccaaaaa cgtatcagcc
     1261 gtttgaaaca aatcaatgca atggaagaag atggaacttt tgaagttcta cctaaaaaag
     1321 aagttgctgg tttgaacaaa gaacgtgaac gtcttgaaaa attcttaggc ggtatcgctg
     1381 atatgccaag aatcccagat gtaatgtaca tcgttgaccc acgtaaagaa cgtattgctg
     1441 ttcaagaagc acacaaatta aatatcccaa tcgtagctat ggttgataca aactgcgatc
     1501 cagatgaaat cgacgtagta atcccttcaa acgacgatgc aattcgtgcc gttaaattga
     1561 ttacttcaaa aatggctgat gctttcatcg aaggaaacca aggtgaagat caagtagttg
     1621 aagaagattt cacagaagaa aacacagcaa catcaatcga agaaatcgta gatgttgttg
     1681 aaggcgacaa ttcttcagca gaataatttt gaatgatgga gctgtttcaa aggctgggcg
     1741 acaagcctca ctctcctttg aagcagcttt ttttaaaaag aaaaacacac aggaggagtt
     1801 aaacatggca gacgttacag ctaaaatggt aaaagaacta cgcgaaatga ctggcgtagg
     1861 aatgatggat gcgaaaaaag cattagttga agtagaaggc gacatggaaa aagcagttga
     1921 tcttttacgt gaaaaaggaa tggcaaaggc tgcgaagaaa aatgaccgca ttgcagctga
     1981 aggattggct tcagttgctg tgaaaggtaa cactgcagca atcgttgaag taaactcaga
     2041 aacagacttt gtttctaaaa acgaaatgtt ccaagattta gtaaaagaaa ttgcagaact
     2101 tgttgctgaa aacaaaccag cagacatgga agctgcaatg aaaatcaaaa cagataaagg
     2161 tacaattgaa tctgacttga tcgaagcaac tcaagttatc ggagaaaaaa tcagcttccg
     2221 tcgctttgaa gttgtagaaa aagacgacaa tgctgcattt ggtggatact tgcatatggg
     2281 cggacgtatt gccgtattga ctgttctaga tggaacaact gacgaaacag ttgcaagaga
     2341 cgtagctatg cacgtagcag ctatcaatcc tcgttatgta aacgaatcac aaattccaga
     2401 agctgaatta gaacatgaaa aaactgttct tacagaacaa gcattgaacg aaggcaaacc
     2461 agcgaatatc gttgaaaaaa tggtagaagg acgcttgaag aaattcaaag cagaaatcgc
     2521 tttggtggac caaccattcg ttaaagatcc tgatatgact gttgaaaaat acgttgcatc
     2581 aaaaggcgca actgttaaaa cattcgtacg tttcgaagtt ggcgaaggta tcgaaaaacg
     2641 cgaagataac tttgtagaag aagttatgag ccaagtgaaa aaataattct gttgaataac
     2701 agacgggaac gcacggtttg tgcgctccct ttttttaagc aaaacaagaa aaaccagtct
     2761 tagagtgtcg aaatcatcta agtatggtaa aatggcaata gaaaatattg gaggaatgaa
     2821 catggtcaca ccgaaatatc agcgtgtagt cttaaaattg agtggagaag ctttagctgg
     2881 ggacgcagga tttggaatca aaccaccagt aatcaaagaa atcatccaag aaatcaaaga
     2941 agttcacgag ctgggcgttg aaatggcaat cgttgtgggc ggcggaaata tctggcgcgg
     3001 acaaatcgga gcacaaatgg gcatggaacg tgctcaagct gattacatgg gtatgttagc
     3061 tacagttatg aatgccttag ctttacagga tacgttggaa aacgtgggtg taccgacacg
     3121 cgtgcaaact tcgattgaaa tgcgtcaaat cgcagagcca tatattcgcc gtaaagcaga
     3181 acgtcattta gaaaaaggac ggatcgttat ctttgcaggc ggtaccggca acccgtactt
     3241 ctcgacagac acaacagcag cattacgcgc ggcagaaatc ggcgcagatg ttatcctgat
     3301 ggctaaaaac aatgtagacg gtgtttattc tgctgatccg aaagtagatg caaatgcagt
     3361 aaaatttgaa gaactgactc atctggaagt tattgccaaa ggattacaag tcatggattc
     3421 aactgcaagt tcactaagca tggataatga tattccactg cttgtcttca atttaaatga
     3481 acacggaaat attcgccgtg caatcttagg cgaaaatatc ggaacaactg taagggggaa
     3541 ataaaaatgg cagatgcaat tatgacagaa gcaaaagaaa aaatgaaaaa agcagcacaa
     3601 aatcttcaac gtgaacttgg gcaaatccgc gcaggccgtg cgaacgctag ccttttggac
     3661 cgtattactg tgaactacta cggtgcgcca acaccattga accaaatggc atcaatccaa
     3721 attccagaag cacgtgtttt aatgatcaca ccttttgaca agagcattct tcaagatgtt
     3781 gaaaaagcta ttatggcaag tgatatcgga atcagtccaa ccaatgatgg aaatgtcatt
     3841 cgcttagtta tcccgcaact gacagaagaa cgcagaaaag aattagctaa agatgtgaaa
     3901 aaagaagcag aaaatgcaaa aatcgcgatt cgtaacatcc gtcgtgatgc gatcgatgaa
     3961 tataaaaaac aacaaaaaaa tggtgatatt accgaagatg atttgcgcgg attagaaaaa
     4021 gacgtacaaa cattgacaga taacagcatc aaagaagtag ataagatcgc tgctgataaa
     4081 gaaaaagaat tattggaagt ttaataaaat cataaaaaca gaggggcgga tcattccgct
     4141 tcctctgttt ttatttatat ttcttttatt taaaaattaa aaaaagaaaa aattattcag
     4201 tttttaaata aaaaaacaat gatttcatag gaaaaacagt tagatattgc tataatactt
     4261 aagatagtgg gaatcttt
//
LOCUS       DAJSMI010000182         4266 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJSMI010000182 DAJSMI010000000
VERSION     DAJSMI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.186"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            170..1471
                     /locus_tag="ON430_002508"
     CDS             170..1471
                     /locus_tag="ON430_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3220393.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYTTFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1498..1758
                     /locus_tag="ON430_002509"
     CDS             1498..1758
                     /locus_tag="ON430_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR3220394.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2757..3992)
                     /locus_tag="ON430_002510"
     CDS             complement(2757..3992)
                     /locus_tag="ON430_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR3220395.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1242 a          676 c          875 g         1473 t
ORIGIN      
        1 tgttgctcct atgatttaga aaacttattt tcaggattct taaaaataaa tttgattatt
       61 taagagaata tattgaaata aaatttctta attatataat tagttatgaa cattctttgc
      121 gttgtcatgc aatgaatggt tcatatttta ttgaaaagga gtggttttag tggaaaagct
      181 gatgaaatgg gtcgaagaga aacttgtccc accaatggca aaaattggta cacagcgtca
      241 tttattagca attcgtaatg gggttgtttc aacattgtca ttgattttga ttgggacatt
      301 ttttatggtc tttatcaatt taccgtttcc tggatggaat gaatttattg ccccttattc
      361 ggcaacgatt gttttaccct ttaggatgac gatgggattg atggctattt atacaacttt
      421 tgttatgggt tcggatctag ctaaatcata tggattagat agtgtgacag gtggtatatt
      481 atctttagga acgttcttta tgttacaagt tcctgtaaac gttttaactc ctgaagaggc
      541 acctcttggc tgggtcttac ctatgagttc tttgggagca tctgggatgt ttgcggggat
      601 cttatctatg atttttgcag tggaagtata tcgtttcttt aaaaatcgaa atattactat
      661 caaaatgcca gaacaagtgc cacctgctgt tgcccgtagt tttgaggcat tgattccagg
      721 tgcagtcatt ttaacaatta cttggctgat tcgaagcgtc gcgggctttg atgtaaacgc
      781 tgccttatta tcgttattcg agcctttaac caatatttta gggaataatt tgcttggtgt
      841 tttactacca atgtttttga tccatttgtt gtggtcattt ggtatccatg gcatgagtat
      901 tgtaggggct gttgttcgtc caatgtggct gatcatgttg gatgagaatg caaaggcatt
      961 ggcagatggt acagctgcca cccaattacc ctatatcgct cctgaacagt tttatcaatg
     1021 gacggtaaca attggtggtg caggagcaac gatcgtggta tctgttttgt ttatattctt
     1081 ctgtaaatca aaattcttga aagaggttgg tcgtttttct attattcctg gtatcttcaa
     1141 tatcaatgag cccataattt ttggagctcc tatgatgttg aatccttaca tgttcatccc
     1201 gttcaactta gttcctttag ttttgactat tatcagttat tttgcagtca aattagaaat
     1261 ggtcaataga tttactgttt tgccaacatg gacattacca gctccaatcg gcggttatct
     1321 ttcagcagga aatgactggc gtgttgttat cttgatcatg atcaacacat tgatagcatt
     1381 tattatctat tatccatttg taaaagctta tgataaaaag atgctgtcag atgaacaaag
     1441 tcgtggagaa aacgaagtcc agactgtcta agtttggttt ttagaaggtg ggaaacaatg
     1501 tattggctga ttttattttt tgtttttatc tttttattaa cggctagtca ccttatattg
     1561 aatatgctag ctacatatca tattcaaatc aatcgatgga tttgggcact ggcatcattt
     1621 ttgatcgtga ttcttccaaa gatcattgta ccgcatatga atgttttgtt ttcatggggg
     1681 acatatgtac tttgtggaat ttttgctatc aattttatga tcgagcagca tagatggttt
     1741 gtcacatcga agctttaaat gttctaaaag tggttgtaac agaagtggtt agcttcaaga
     1801 aataagaaga aatttacgaa aattgcttct taaatttttg tgaaatgttg gcttattttt
     1861 cgtttggttg aacttattac cgcagatgca agcttttgac caccgtttat tcggaatatg
     1921 ggactgtgac aaggcttttg tcacagccct tttttacata aagattatta ttgttttaac
     1981 tgttttggca gaaagaagga ggaaaaaatg gaaattttat cagcaatgga tttagaatta
     2041 gtagatctac taccaaagtg atcaagtcaa atatagattt gggagcttac aaccatatag
     2101 taggggctgc agtatggtgt atgaatggta atatgtatga tgtgagtgtt tattcaaatc
     2161 atgaaacgtg tatagaaata attgtgctgg gaatagcaat cagtcatggt gaacgtgact
     2221 tttagtgtat cgttgctgac gacatagaac attctgacat gatctactca atatgtgggg
     2281 attgctggca gtttatatag gagtatgcat cggattgcga gatcattgtc tcaacagaag
     2341 agggaggttg attatcaaag ataaagtggt aaatcctaca tttgatttag taattgttga
     2401 ggggactagc ggcacaaatc tctacaaagc gatgcttcta gatctatatg acacatttta
     2461 agaaaatgta tttgcctaat tattatggtc tttcttttga agaaacgttg agatgatcca
     2521 accagagaaa ggtgtcaaag aatttgacta agaataaatg gcagacgcta cctatgctca
     2581 tttggatagc gtcagatctt ttattttaag gatgattttg tgttttttag acaccgtaat
     2641 ttaagtaact ctaacttttc aatccactat ttactttttt tgtattttca ctgtctgggt
     2701 ctcgaatagc gaaattactg atgagtctct attaaaaaag ctttttctac ttgaaactat
     2761 attgggtggt tgctcaggaa ggtttcgatt tcttgaattg cttgttgtgg accatttatt
     2821 ggaaaaacgg cttgcagcgt gcgtgcctgc tgacgaatct tgggtgaaga aacttcgcgc
     2881 agagcaatct gcagttttct ttcagtcact ggttttgtga gggcgattgc gtttccgtat
     2941 tttacacaat agtcaatatt acagctttgc tcaaattgca tccccacacc gataaacggt
     3001 ttgccggatt cacaggcagt ttggacggtc ccttccccgc cgtggataaa agaaaaatca
     3061 atgaattcat gcaaaagatg actagacaga tattctgtta ttaaaagatc ttctgagtca
     3121 gattgaatgt catcaagcag atagtcaata ggtgcaataa ccgctagatc cggttgtttt
     3181 tgtaagaaga acaaaatttt gcgtaacata cgcggattgc ctgaactacc cattgcgaaa
     3241 tagataatac gttttctttc ttgttgcttt cgctgtataa aagttagcac ttcttcaggc
     3301 aaggaagtac tgagcttagc aaaaattgga ccaacgattc gataattttc cggcagctgt
     3361 tcttcttcca aaaatagttt aggcgttgtg atgagattta gatcagcatc catcatttgt
     3421 aaacttgttt ttggtaaagg aagcatttct ttttgtgcag cgtgtttgaa ggcattgggg
     3481 atccatcggg tatttactat acgctttttt gtccagtgcc aaaggggatt gcttaaaggg
     3541 ccgaagaaac gtaactcgtg gggcatcagc gcatgctctg attgaaaaaa aggttcactc
     3601 aaagcatagg gtttaacata tactaaagga atctttttat tgcgggccga taaaaaaatt
     3661 gtcacattcg aacctgtcac aatcagtcga ggctcaaact tagcaatcag actctgttca
     3721 ctagtgatac gagaggcaac catttctttg gtaaaagggt ttttaagtgt acgcaactgg
     3781 tcaaatttta tgatttgttc ttcttgtttt ttcgtaagga tcggcgctag taaatgatac
     3841 tcaaagccac tttctgtgat caaaggagca aaagttttag aaaagccaaa gaatacacag
     3901 taataacggt ctttcatagc tttggcaacc tcaatcattc gtgttgtctc agctaaattg
     3961 aatactgcag gagcaaaaag aattgtcttc atgaaatcct ccttattgga atgagtctat
     4021 cccttcgttt cttactagta tactaaaaaa cacagcgttc gagtagccta tgtatatttt
     4081 gaatattaaa ctgtctctcg ctgttttcac aacttctaaa tttaaatttt gtaatcaagt
     4141 tgaaactcca attttcaaaa aaacacgcaa caattttttg atcgatttat atgttgtgcc
     4201 agataaatac gagaagttgt gtcattaaaa ataaacatga gaacataaaa aaataaaata
     4261 attata
//
LOCUS       DAJSMI010000183         4227 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJSMI010000183 DAJSMI010000000
VERSION     DAJSMI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.187"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..377)
                     /locus_tag="ON430_002511"
     CDS             complement(<1..377)
                     /locus_tag="ON430_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3220396.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERLVS
                     CGMITLYRLFSKGIFDIDTLPMKG"
     gene            complement(474..1139)
                     /locus_tag="ON430_002512"
                     /pseudo
     CDS             complement(474..1139)
                     /locus_tag="ON430_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1062 family transposase"
     gene            complement(1221..2675)
                     /locus_tag="ON430_002513"
     CDS             complement(1221..2675)
                     /locus_tag="ON430_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3220397.1"
                     /translation="MFSKEESSMGYKELGKDILANVGGSENVSSLAHCATRLRFNLKD
                     DSKADESAIRNLKGVVGVVNQGGQFQVIIGGDVKFVFAEIDKLGDFSASKESSSEANN
                     KGIVSKVLDTIAGIFVPIVPALTGAGMLKALLALLVMLEWMTPESQTYQFLNFIGDAA
                     FYFLPVLLASSAAKKFGCNQYIAITLGAILLHPTFTAMIAGARETGESLALFGLPVTL
                     ANYGSSVIPIILAIWFMSYIEPIIDKLMPNAVRIFMTPLLTLLIVAPITLIVIGPLGT
                     FLGNGLGSLIEFVNTYASWLVPLLVGAFTPLLVMTGMHYGLIPIGINMLATSGFDTVA
                     GPGMMVSNIAQGGAALAVAFRTKQLSVKQLAVSSGLSAFMGITEPALYGINMRFKKPL
                     IASMVGGGAAGLFIGIMGVGRYAQVAPGILALPSFFGEKGLSNFIYAAIGCAIAFVVG
                     FIASFILGIDEETEEVKEDTSEIPVNIASDNRRL"
     gene            2954..>4227
                     /locus_tag="ON430_002514"
     CDS             2954..>4227
                     /locus_tag="ON430_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3220398.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHG"
BASE COUNT         1329 a          847 c          712 g         1339 t
ORIGIN      
        1 cctttcatcg gtagtgtgtc aatatcaaat attcctttag aaaataaacg ataaagtgtt
       61 atcataccgc atgaaacaag cctttctttt cgcccgataa tgacgtcagg cgtccaacca
      121 agagtgactt tctctttaat gtaatctact tcatgagcag gtaattgaat gacttttcta
      181 ccacattttt tcttattttc tttatactgg tgccaataat caagagcagt cttgccttgc
      241 ttgaacttat tgactacatt ataaatagtt tgtatagcac gtcccattcg gttagcgatt
      301 tcaaccggct tattatgttg aagataatat gattctatca ttgtcagttc gtctatggta
      361 agatgtttgt aggtcattta tggttacact cctttgtttt ctttcgtcgg aaatacaatt
      421 tgagtgtacc ataaatgcct ttttattttt ctaacttaat tttacaatta gcgaaaagtc
      481 attgatttga atagatgctt aggtactgat tgcatccatt ggttcaaacg attttcaata
      541 tctatagctc gtctgccttc tggctgaaga gtgatgatta ctttagataa tcgctcaacg
      601 agggtaataa cagcactttt gtgcttttta cccacaatag tatccccttc aaggtggcca
      661 aattcttgat tgaatttaga ataatcattt cctctgtcac gaagtgatcg acgaaaagat
      721 tgttttcccc gagtttcttt gtgtccgttg gctttacgtt ttcctttcat aggtaggtga
      781 gtcacttcaa atacgccttg tttaaacata cgataaagtg tacgcataga acaagaaata
      841 gaaaactctg cacggccaac aatcacatca ggtgtccaac cttgaacaac cctcttttga
      901 atatattctg tttgtttatc tgataagaca ataggacgtc tgccacagcg agttttattc
      961 ttcttgtatt gattgaaata atctagagca ttgtgccctg ttttgaaaaa ttggtaaact
     1021 ttatgaatcg tttgtctaga tctattcaat agtactgcgg ttttggctac agattgatga
     1081 ttattataat acgcctctat cattgcaagt ttatttgatg taagatgggt ataggtcatt
     1141 tgtcttcact ccttataatt cttttgcggg aactatcttg agtgtagcat aaatgacttt
     1201 tttatttgtc tagcttaatt ttacaatcgg cgattgtcac ttgcaatatt tactggaatt
     1261 tcgctcgtat cttcttttac ttcctcagtc tcttcatcaa tacctaaaat gaatgaagca
     1321 ataaaaccga caacaaatgc aatcgcacaa ccaatcgctg cgtaaataaa atttgatagt
     1381 cctttttccc caaagaaact tggtaacgca agaatgccag gagcaacttg agcatatcga
     1441 ccgacaccca tgattccaat aaatagtccc gctgctccac cacctaccat tgaagcaatt
     1501 aacggctttt tgaatcgcat attaattccg tataaagctg gttcagtaat tcccataaaa
     1561 gcagacagac ctgaagaaac agctaattgt ttgacagaaa gttgttttgt tctaaaggca
     1621 actgctaagg cagcaccacc ctgagcaata ttggaaacca tcatacctgg accagcaaca
     1681 gtatcaaatc cagatgttgc taacatatta ataccgattg gaattaaacc gtaatgcata
     1741 cctgtcatca caagtaatgg agtaaatgca cccacaagta atggaaccaa ccaacttgca
     1801 tacgtattaa caaattcaat aagtgaccct aacccattac ctaagaatgt acccaaagga
     1861 ccaatcacaa ttaaggtaat tggtgcaaca atcaacaatg ttaacagagg cgtcataaat
     1921 attcgaacag cattgggcat caatttatcg ataatcggct cgatatatga cataaaccaa
     1981 atagctaaaa taatcggaat aacagaagaa ccatagttag ctaacgtcac aggtaatccg
     2041 aataaagcta aggactcacc tgtttctcta gcacccgcaa tcattgctgt aaaagtcgga
     2101 tgtaacaaaa ttgctcctaa ggtaattgca atgtactgat tacatccgaa tttttttgca
     2161 gcagaactag ctaataagac cggtaaaaaa taaaaggctg catcaccaat gaaatttaaa
     2221 aattgatagg tttgcgattc tggtgtcatc cattccaaca tgactaatag agctaataat
     2281 gcttttaaca tccctgcacc tgttaatgcc ggaacaattg gaacaaaaat acccgcaatt
     2341 gtatctaaaa cttttgatac aatcccttta ttgtttgctt ctgaactact ttctttagaa
     2401 gcggaaaaat ctcctagttt atcgatttcg gcaaatacaa attttacatc tccaccaata
     2461 atgacttgaa attgaccacc ctgattaact acacctacaa cacctttaag atttctaata
     2521 gctgattcat ctgctttact gtcatctttt aaattaaatc gaagtcgtgt tgcacaatgc
     2581 gctaaactgc ttacattttc agaaccacca acatttgcta atatgtcttt tcctaattct
     2641 ttatacccca tgctactttc ctccttagag aacattttct cttttgataa tttgaatata
     2701 gcgtgctatt aatagataaa caatacattt gaaaccgttt tacacagcgt tgcgaaattc
     2761 attcagttta cgattatttt ttcgcaacac tgttccaaaa aaataaaatc cacttactaa
     2821 ctaatcgtta aggctctata ctttttgatg ttggtgaact tcaccaacat cttttttgta
     2881 taaaaaaata tagaaaagac accctgaaat cctttagaat taatttggct accaattcat
     2941 aggaggattc aagatgtctc atactcagct tatcaaagat accctaaata ttctagacct
     3001 aaatatctat ttcgaggaaa attgtttaac taaagaaaaa tacaaagggc aaatttgctt
     3061 gatttacaaa ggaactcttc tgtacaagcc agaagagtgt tcccattgtc tttgtgtcgt
     3121 cccctctagg atcattcgtt gggggacgac aaccgttcgg ttgttactga atgatgtgtc
     3181 tgagtatcgc acttatctgg agctgaagaa acaacgattt aaatgtaagt cttgccaaag
     3241 aacattcgta gcggatacct ctgttgctga aaaacactgt ttcattagtc aaaaagttcg
     3301 ttggtctgtc attgctcgct taaaagagaa tacttccatg acggaaatcg ctcgtcagaa
     3361 aaatatttcc acttcttcgg tctatcgtgt catgaagcga ttctaccgcc cattgaatcc
     3421 tttcaaacag actttgccaa aggtcttatg ctttgatgaa ttcaaatcgg ttcgaagtgt
     3481 tgccagtgca atgagtttta tcatgatgga cggacaaagc catgcattat tggatattgt
     3541 cgaaaatcga cgcttgcctt atttagaacg ctatttttct cggttttcat tagcgactcg
     3601 tgaagccgtt caattgattg ttattgatat gtatgcgcct tatgtttcgt tggtgaaaaa
     3661 gcttttcccg aatgctcagt tgatcatcga tcgtttccac atcgtccaac atattggcag
     3721 aactttcttg aatcatcgag tgaaagaaac cacggtacga cttaaaggaa attcccactc
     3781 tcaacgaggg ttagggaaaa aattgaagcg ctattggaaa ctcctacaaa aagaagaagc
     3841 aaagttaaac tatgaaaaac gggtttggcg tgccagtttt aaagattatt tgactgaaag
     3901 tgagattgcc gatcgactgt tagctgcttg tcccaactta cgccaaggct atcagttata
     3961 ccaagacatt ctttatgcgg taaagaaaag agatcagtca ctatttgagg attgtttaac
     4021 tagagacatt agcggtctgc cagaaaccta tacaaccact ttacgaacgt ttaaaaagtt
     4081 tctgccacag atccaaaatg cgcttcacta cagctattca aacggaccat tggaatgctt
     4141 aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttcgcaact tttacaattt
     4201 caaactacga attatgattc gtcatgg
//
LOCUS       DAJSMI010000184         4193 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJSMI010000184 DAJSMI010000000
VERSION     DAJSMI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.188"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..522
                     /locus_tag="ON430_002515"
     CDS             <1..522
                     /locus_tag="ON430_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCR3220399.1"
                     /translation="LVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISYNYPRK
                     GIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVVSWVLMQ
                     LGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGIWGIISVLVVASLSMIG
                     VLIGRKVLKKYKK"
     gene            632..2386
                     /locus_tag="ON430_002516"
     CDS             632..2386
                     /locus_tag="ON430_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3220400.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            2346..4112
                     /locus_tag="ON430_002517"
     CDS             2346..4112
                     /locus_tag="ON430_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3220401.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
BASE COUNT         1391 a          589 c          797 g         1416 t
ORIGIN      
        1 ctagtaattt actttttttc aataatggtt agtagtattg gtggggcgtt tggtcacagc
       61 atatccccag ggatttttgg attattatct ggaatcatat ttgtttatat tagctataac
      121 taccctagaa aaggaatttt tactatttac acaattgttt tattgttatt ttttacgtta
      181 atgggcgggg cgtacttacc ttggtttatc agttcgatta gtatggcgat cttagctgat
      241 attattctat ctgtatttgg atatgatcga gcaattcctc aagttgtgtc ttgggttcta
      301 atgcaactag gcagtgctgc cggacagtgg attcccatat ggttctttac agatcgtttt
      361 cgtcaagact ggatagatag aggacaatct gctgcaacaa tggatgcaat gatccactac
      421 gcggtgggta tatggggaat tatttcagtg ttagttgtag caagtttatc tatgattggt
      481 gtattgattg gaagaaaagt cttaaaaaaa tacaaaaaat agaacgtagt ttaccttcta
      541 tctgtttgta catctctaat ttacgctatt acggaaagca ttaacagttg taaacgaata
      601 agagaagaaa ttgaatgcgg gggaggaaaa aatgaaagta tacaaaaaat tatttagtta
      661 tgtccctaaa atgaaatact acggatggtt agccacttta ttttctgatt tttctgtcgt
      721 attaacagtc tatggatatt acagtattta caaatttctt cagtctctaa ttattgtagg
      781 agatgaatat ttagctaaga gttatgcggt tcaaactgtt gcttggttaa ctttaggagc
      841 tttattttat attttttctg gcttattttc tcatatcctt gggtttcgct tagagactaa
      901 tttgagaaaa aagggaatta gcggtttaac agaagcaagt tttcgttttt ttgatttaaa
      961 ttcttcggga tatattcgaa agacaattga tgacaacgcg gctaaaacac atatggcaat
     1021 tgctcacttg attccagata gtgctcaagc catagtaaca cctgttttat cagttgcctt
     1081 aggctttttc attaacttta aaattggatt ggttctaatc gccatgttag taattggtgt
     1141 attcttgtta aagaaaatga cagagaatac aaattttatt caaaaatatc aagaatcgtt
     1201 agatgtgtta agctctgaaa cggtagaata tatccgcgga attcaagtta taaagatatt
     1261 tggtgctaaa gtaacatcat ttaaagcctt gaataaagca attaacgatt atgctaagtt
     1321 tgcatataag tattctttga gtgggaaaac accttttgtt ctttttcaat ggctattttt
     1381 tggcatagtt tgcattttga tagttcctat aacctttttt ataacttctc ttaccactcc
     1441 caagatctta gctgttgact tgttgatgtt gtttttttta agtggaatta tttttgtttc
     1501 tttcatgaga ataatgtatg taagtatgca cttgtttgaa gcaagatatg cagtcgaaac
     1561 actagactcc ctttatttag aaatgaagca agaccaacta aaatatggga acgagacaca
     1621 gattaaaaat tgtgacattg catttgaaaa tgtcagcttt tcatacaaag aagagtgtgt
     1681 tttagaaaat ttcaacttag ttttagaaca aaaaagaatt tatgcattag taggaaaatc
     1741 aggaagcgga aagagtacga tagctaaact aatttcaggt ttctacaatg tggataaggg
     1801 acagattaaa attggtggta agcctttaaa tcactattca aaagacacaa ttattaacac
     1861 aattgctttt gtctttcaag atacgaaact attcaatcag agtatttacg ataacgtttc
     1921 tatagcaaat cctcaagcgc cagcaagtaa agtatttgac gcgctaagat tagccggttg
     1981 cgaatcgatt ttagaaaaat ttcccgaaag agagcatacc ttaattggta gtaaaggagt
     2041 ttatctgtca ggaggagaaa aacaacgtat agctattgcc agagcaattt taaaagatgc
     2101 aaagattgtt attatggatg aagccagtgc atctattgat ccagataatg agtatgaact
     2161 acaaaaggct tttcaatatt tgatgaaaga aaaaacagtt attatgatag ctcatcgatt
     2221 atccagcatt aaatctgtag atgaaattct tgtcttgtca gaaggaaaaa tagtagaacg
     2281 tggaaatcat aatgaattaa tggctaaaca tcgagtgtat aaacaattgg tcactaatta
     2341 tgagaatgca aatgattgga ggcttgacaa tgaaaaattt ttataaaaaa aaatttgctt
     2401 taactgatca gggcgcagaa gctttaacta aagcttctat ttctagtttt tttgtatact
     2461 gcattaatat ggtaccagca ttcattataa tgatgctgat agacgaatta gttttagaaa
     2521 atgcaaagcc ccgttggctt tattttgcag tatcgtttgt tactttgctt ttcatgtatt
     2581 ggttgttaga tagggagtat gaaaacttat ataatagtac ttataaagaa agtgcgcatt
     2641 taagagtgca aattgcagac gatttgtcaa atttaccatt atcctatttt tcaaaacata
     2701 atttatcaga tttatctcaa actatcatgt ctgacgttga aggtattgag catgcgatga
     2761 gtcatgcaat acctaaagcc ggcggtatgg ctctgttttt cccttttatt tcagtgatgc
     2821 ttttggttgg taatgtcaaa atgggattag ctgttatttt gccaacgtta tttagttttg
     2881 tcttaatctt gttatcaaag aaatcccaaa cgaaagccaa tactaaatat tacgatactt
     2941 tgagagaaaa ctcggaagaa tttcaagaaa ctattgaatt gcagcaagag attaatagct
     3001 ttaatctatc taaaaaagtt caagacagac ttttcaaaaa aatggaagag agtgaaagga
     3061 ttcatttaaa ggtagaatta agtacttttt cagtcatggc cttatcctct attttctcat
     3121 atgttagttt agcagtagtc attctagtag gtgttcactt actgttaacg ggtgaagtga
     3181 ctatactcta cgtcgttggt tacttactag ccgcaataaa aataaaggat tccttcgatt
     3241 ctatgaaaga agcagtgctt gaaatatttt atttagctcc taaaatacag cgaattagag
     3301 ctatgaagga aaccagtatc caagagggat cagatagtcc tctgaaatct tttgacattg
     3361 agttgcgaga tgtctctttc tcatatgata acaatacccc aattttagat catatctctt
     3421 ttacggctaa acaaggagaa gtgactgcgt tagtaggtgc tagtggttcc gggaaaacta
     3481 gtatcttgaa attagtgtct agactgtatg attatgatga aggctgtatt ttaattgatg
     3541 ggtatgatat taaacgtgta tctccagctt ctttgtttag caaaatcgct attgtttttc
     3601 aagaggtaac tttgtttaat acaagtattt tggaaaatat acgtattgga aatagtcaag
     3661 cgtcagatga ggaagtgaaa aaagcagctc gtttagctaa ctgcgaagac tttattgaaa
     3721 aactacctga cggttatcat acattggtcg gagaaaacgg aagttcactt tctggaggag
     3781 agcgacaacg cctatccata gcacgggctt ttttgaaaaa tgcgccaatt ttaattttag
     3841 atgaaattac tgccagttta gatgcggaaa atgaaaaacg tattcaggaa agtttaaatc
     3901 gtttaataca agataagaca gtactaatta tatcgcatcg cttgaagtct attgaaaagg
     3961 taaataaaat tgtagtgatg gatcaaggaa aactagttga tcaaggtact cataatgagt
     4021 tgtatcgaag gtcagaaata tacaaaaatc tcataaagaa aactaaacta tctgagaaat
     4081 tcgtctatga aaaagaagct caatcacgat gatgaaatag gctttaaatt aacctacaat
     4141 ttttgggata tagttataat tctttttcaa aaagaataat tttattaaac agc
//
LOCUS       DAJSMI010000185         4131 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJSMI010000185 DAJSMI010000000
VERSION     DAJSMI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.189"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            438..1931
                     /locus_tag="ON430_002518"
     CDS             438..1931
                     /locus_tag="ON430_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCR3220402.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2066..2362
                     /locus_tag="ON430_002519"
     CDS             2066..2362
                     /locus_tag="ON430_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR3220403.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(2386..3345)
                     /locus_tag="ON430_002520"
     CDS             complement(2386..3345)
                     /locus_tag="ON430_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3220404.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            3528..>4131
                     /locus_tag="ON430_002521"
     CDS             3528..>4131
                     /locus_tag="ON430_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220405.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDEL"
BASE COUNT         1362 a          665 c          802 g         1302 t
ORIGIN      
        1 tatatttgtg cgttttgcgt tgacaattta gagtgaaaag aggtaaactt aattctatag
       61 aaaccacaga taattcacta tttacaaagt agcaaaagca gtggtactct ttaaacagaa
      121 aaattacata aaaaaaagag acggaaataa ttgattttag cagaatcaat tatttctgaa
      181 tcacatggtt aggacatgtg ttcacgctcg tctcaagaat atgtaagtag ctaccaacta
      241 cttatattaa aagttgatcg gatacctttt ccgattgcct ttattctacc aaaaaaaggc
      301 gagcaactca acagaagaac gtttatttag tgtacttaaa ttaaatattc ttaaacactg
      361 aagagagaac acatactatg gctgagatta aagctaaact gtgttctctt tttgtttgag
      421 aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc tattcttaaa aacggcttac
      481 gtacatacaa atatcgaaac agcaccatga aacctgcagg tcatattgat tccagtagga
      541 gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa tgttgggcgt ggtatggtca
      601 ttacttcgga agaagctatt ttagagaacg aaaagaaact aacgcattgg acacctaata
      661 cctatcgctt tggtacttat gcggatgatt atcgaacagt ggttaaaggt cattctgaga
      721 aaaacttaag tcaaatcaac acatttgtag ttgatatcga ttcaaaagag aaccatcaag
      781 gcgaaattat tttagcctgt ttagatcaag tgggctacat gccaacgcta attttagaat
      841 cagatcatgg ttatcaagtt tattttgtac taaagacacc aggctatgtc acaaagaaaa
      901 gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa aacaattcgt ctacagttgg
      961 ctgaaaaatt gtccggagtg gatctaggat gtaaccactt cggaattgct cgattcccac
     1021 aagaaaataa tattgttttt aaagaaatga actatcagta ttcttttgct gactggattc
     1081 aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga agatcgagaa cgaaaactaa
     1141 ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga accgtggttc gatatgttac
     1201 ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg tcgaaataat gtgattttca
     1261 cgctttcttt agcttattac tcttcaggtt atgcattgga tacttgcgaa tacaatatgt
     1321 tcacattcaa cgaacggtta caggaaccgc tatctgaagc agaattagta aagattgtac
     1381 gtagtgctta ttctggtaat tatcaagcag caagcagaga attcattatt gaattgtgcc
     1441 agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt cattcaacga cgtggctggt
     1501 ggaaatttaa aaagccacga gatcaaagaa aatatagtca taaaaatgaa tggaaagaag
     1561 atctattggc ttatctaagc gaaaaaagtc ttgctagtga gccttatttg aacctgtcaa
     1621 aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac acttgataag atcttaaatg
     1681 agttacgaga ggagaataaa gtcttctaca aggtaacacg aggaagaaat ggcggaatga
     1741 tcattgcttc agtcaaaacg ttgttcgcga gggttattca actaagtaaa gaaaagagac
     1801 tagattattc aacaaggatc acagaggtat ttggactcaa cgagagctta gtagaagaga
     1861 catttagaca attatcaacc cccttgaacc ctcttaaaga agtgaatctt tttgaagtag
     1921 atacaggttg aaaaataaaa ggtgcaaatt gccttataca atatatctat gatacttctg
     1981 taggtgttgc tccctttgcc tagctacgtg taggtggtat acttataact gcgtatgcag
     2041 tcttatgtat catggaggaa gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt
     2101 taatgtgttt atcattggtc gtttgggatt atctgtggaa gtgttctgtg agctttatgg
     2161 ttttaagcag gggactttgt cttcttgggt taccagggaa aaaacagttg cttctttgcc
     2221 gattgagttt cttcatgggt tgtccttagc ttctggggag actatggatc aggtatatga
     2281 ttgtttgtgt gtgttagagc aggagtatat tgagtataaa atagctaacg agttaagaaa
     2341 aaggaagaag tatatccagt agtagttttt atttactctt aattgtcaaa ttaagttaga
     2401 caaatcatca atacttacgt aactcaaaaa tacttccaaa ggtgttcgat aattcagtga
     2461 ttttctagga atattatttc gtttcgatgc gacagattga ataaaagatt catctactga
     2521 attgaaatcc atagatttca ataaaccatc tctacgtaac aatccgttag aattctcgtt
     2581 taggcctctt tgagacggcg ttcctggatc agcgaaataa atggcaatat cattggcatt
     2641 actgatctgt ttccaatttg aaaattcctt tccacaatca aaagtgatgg atttgaatag
     2701 atttttcggt acagattcaa accaatgatt taatttgttt tcaatatcaa tcgcttgtct
     2761 accacacggt ttcaatgtga tgataacttt tgataatcgc tcaactaagg taattacagc
     2821 acttttatgt ttcaaaccta cgatagtgtc accttcaagg tgaccaaact catttgagaa
     2881 ttggctataa tctttttcac gttcatgaat agagcgacgg aaagcttgtt ttccacgtct
     2941 ttcttgatgt ccatttggtt tacgcttgcc tttcatcggt aagtcagaag gattaaatag
     3001 cccctttttg aacatacgat aaatggtacg agcagaacaa cgaataggaa acgctgcacg
     3061 accaacaatc acatcgggtg tccatccttg aacaaccttt ctttgaatat attctgattg
     3121 ttcctcaggt aaaacaagcg ggcgtctacc acagtttgat ttgtttttct tatactgttg
     3181 atagtactct aaggctgatt ttccttgctt gaagaacagg tatactttat ggatcgtttg
     3241 tcttgaacga ttcaagcaat gcgcagtttt cgcaacagat tgatttattt tgaaataaga
     3301 ctctatcatt acaagttcat ccgttgtaag atgggtatag gtcatttgta ctcactcctt
     3361 ataattcttt ggacggaact attttgagtg tagcacaaat gactttttta gttgtctagc
     3421 ttaattttac aatcggcgta cttaaaaaat tggttcattg agcaacctat cgctgctaaa
     3481 gtagcaaagg aaaacgttaa aggaaagaat ttagggaaga aatcgaaatg aaatcggacg
     3541 actggattga acacacctgg cttcctctga aaagaaatag caaacgaaag aaattttggg
     3601 tattgcaatt atggccatta ggaaaacttt gtagttacgg ggcacgactt attcttgatt
     3661 taaccgttta ctcgattgga gtatttggaa cggcttatta cttgataaat gatcccaata
     3721 ggattgcagc atcagacatt tttacagtgc taatgacgtt gatttttgtc atgctattgt
     3781 ctatttttac tcaattagtc atgcaattta ccaggcaaaa attaacagca ggaaaacttt
     3841 tcaaaagcct attcgattta attgttgtaa atgcaattct agcgacttta ataaaacaaa
     3901 ttgaacaatc tggaagaaca acaaaaggag tgaaggattt tttattgacg tttattcact
     3961 ttgtaaatgg acacatgtat ttgtttgaag tgggtattat tctctttttt gtcgggtatg
     4021 tatccacgaa acttattaaa cgttcaattg aaaaagaaat tgaaattgac ctgattccga
     4081 cacttcttgg ttatgagtac aatgttgatt ttggaagata tgacgaattg a
//
LOCUS       DAJSMI010000186         4086 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJSMI010000186 DAJSMI010000000
VERSION     DAJSMI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.190"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..156)
                     /locus_tag="ON430_002522"
     CDS             complement(<1..156)
                     /locus_tag="ON430_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220406.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAEC"
     gene            complement(364..1290)
                     /gene="glsA"
                     /locus_tag="ON430_002523"
     CDS             complement(364..1290)
                     /gene="glsA"
                     /locus_tag="ON430_002523"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR3220407.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1313..2740)
                     /locus_tag="ON430_002524"
     CDS             complement(1313..2740)
                     /locus_tag="ON430_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3220408.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2771..3985)
                     /locus_tag="ON430_002525"
     CDS             complement(2771..3985)
                     /locus_tag="ON430_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR3220409.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1274 a          883 c          738 g         1191 t
ORIGIN      
        1 acactcggct agactttcta gatcgtgaag gatcgttggt gtagtcaccc ttagagaaat
       61 agctgtttgt tcgatgtcta tttcttggaa ttcaaataat tgttcacaca gatacacttt
      121 tctaaatata tttcgctcga tgtaattttc cagcattcct gatacacgcc tccagttttt
      181 cttatgattt tatttaatca tatctgtaaa tcgtattttt gtttcctttt tatttagaaa
      241 agccctttca aaaaccaata aaaaaagaaa ctgggacaaa agtcaaacaa gctcacaatt
      301 tcagcctcat tgtaggtagc tttgagccgt ttggactttt accccagcct ctttcaagaa
      361 tggttattca aagatgtcag cgtctagttc tttgactaca tctttcaaca attggatacc
      421 agctgcgctg ttaccaaatg gatctagtgc cgggctgaag acacctatgc cgtaacgatt
      481 tggcacagct gccatcaacc cgccgcctac accgcttttt gcaggtacac caacatggac
      541 ggaaaactct cctgattcat catacaaacc agcagttgtc ataattgatt tgacgattgt
      601 agcactttct tcagtaatga tttgtttgcc attccaagga gcaattcctt tatttgccag
      661 cacagcagct aattttgcta tgcctgtagc ggtgacattg agggagcatt gtctgaaata
      721 agcatcgagt atctccggta cgtctccttc gatcatttga ttacctttca tatagtaagc
      781 aagagagcga ttgatatcac ctgttcttga ttcagaatga tagatttctt cattcaatgt
      841 gatattagga tcatcacaga tctctttcat aaatgacaag attcgctcaa atttttcatc
      901 tgcatcttta ccggcaatca acgaggttgt tgcaatcgct cctgcattaa caaaaggatt
      961 cattggatgt ttgcgatgat tgatttccat attcatgata gaattgaagg caaaaccggt
     1021 tggttccgtg ttgattgttt gaaagacttt ttctattccg ttgtctttga ttgctaataa
     1081 caatacggga acttttgaca tgctttcgat ggcaaagcgc acatcggcgt ctcctccctc
     1141 aatcagttgt tttgtatcca aatcataaat cgccatacct aattgtttgg gatccacttc
     1201 agctaatgct ggaatgtaat ccgccacttg tcctgtttga taatagtgga aattttttcc
     1261 aatcaattgt tctaattctt ttacttccat ttgaataacc agctttcatt ttttattttt
     1321 ctgcatgctg gaatttatga cggcatgcgt agatcgtaaa tggaataact aagacgataa
     1381 gataagaaac aatcagaatc gtttcataaa tcccatgctg tccagctgcg atactagcag
     1441 gtggaacaaa tgagatacaa aaagcaaaaa gtgaagtcaa aagacctaaa caagcaacga
     1501 ttacttttac aacttttccg cctggaacct gataggctct ttcaagactg tcatctttaa
     1561 aaaccagctt caaatagccc gcgaaaaaca taaagtacaa catcaggtag attacaacag
     1621 tcaatgacat cgctgcaaga aatgaaagat tattcccccc gccgcttaat gtaaagacgg
     1681 ctgcccagat tgttgcaacg atcccatttg cgatcatcaa atgagcagga acaccgtgtg
     1741 tgttttcttt tgcaaaatat tcaggaagta atccttcttc tgctgctacg tgaagtgctt
     1801 ttgaaggaga ggttacccaa gaagcaattt ctccgatgac acctaaacaa acaagaatcg
     1861 caagaatttt cgcaatccat gcaagatttg gatcaatgtg tcttaatagg aattctaacg
     1921 attgaatcac ccctgtatta agagacaaac catgttgagg tactgtagta gcgacggtca
     1981 atccgccaaa agtatctaaa atgattgcca gaataaccaa tacaaacatt gctaatggat
     2041 aatccttttt agggttttcc atttcatttg catgactggc agatgtttct acgcccatat
     2101 atgctaaaat aaaggaaaca aaaacaacca gcgtaggcaa tttcgaaaaa tcaggaatca
     2161 atgttttcat agacagtatc gtttgtagtg gagcgcctga agcgacataa tggatcccta
     2221 agccaaacaa gactaatgct ggcaacaaga taccagctac aaaaccgatc cgagcaatca
     2281 atgaagtgta tttggttcct tttaactgtg agagtaaaat agcccagaaa ataatcaata
     2341 ggattgccaa tttcagatat ttattgtttt gtatagcacc aatacccgtt gcataggata
     2401 atgcaccagt taagaaatac agcatggtca agtagccaac tgttatctca aaccattgga
     2461 aaaagattgc ggcaaaaccg aatcgttttc ccaatgtgcg actgacccaa gtatagacac
     2521 cgcctttttc ccatcctttc accgttgcca tctctgctgc gcacaatgcc acagggataa
     2581 accatagcaa accgcctaat aacaagaaaa atactaagct gaagcctgag gtcgcgaatg
     2641 tgggatattc atataatgaa acgaccattg agatggtcat agccaaaaag ccaaagaacg
     2701 ttaatttttt tgcaggaatt ttcgttttgc tttgttgcat gaatcttcct cttttctatt
     2761 tgaatttgtt tcaattgagt ttgttgccaa agaagaaaaa gtccaatcct tcttcttgtt
     2821 cttcttttgt agtagtgatc gttgtaaagt gcatcgccga ttttaaaatc actgttgtca
     2881 gcacataggc atacacggca gtaatgatga caccaatcac ttgaatccct aagaagtacc
     2941 agctgcttgg attcgttagt aaatggctgt tagatgcaaa gatccctatc agaatcgtac
     3001 ctgtgaatcc gcccatgcca tgaacacccc acacatcaag tgcatcatcc cattgtttct
     3061 ttttacgaaa atcaacagca gcatgacaga cgatacccgc aatgatccca atcagcatgg
     3121 cactttttgc ttctacataa ccggcacaag gcgtaatggc tgccaatcca gcaacggatc
     3181 ctgttagtac gtcgacaaaa tcaaagccat ttccttttac ttttgcaatg atcagccaag
     3241 taatcatcgc aaatgcaaga gcgatgaatg ttgaaccaaa tgcattgact gcttgttcat
     3301 ctgctcgaag tgcaccaccg gaattaaaac cgaaccagcc aaaccaaagc agtcctgtac
     3361 cgatagcagc tgccataaga ctgctatgct gacttctttc tcctaaagga atctttcttt
     3421 gaccaagtgt gaggatacat gctaaagagc cgaatccagc tgtcgtgtgg atgactgctc
     3481 caccagcgaa atctctaaat cccatctgat ccaaaaatcc gccaccccat atccagtggc
     3541 aaacagggaa ataaatcagc agattccaaa aaataacaag tataatatag ccttttaaat
     3601 tcaatctccc tgcaaaagct cctgtcatca aaggaacggt aatcacgata aacatcagtt
     3661 ggtaaagaaa gaacattaaa aatggaatcg ttgcaccatg aaacatgttg gggaagcttg
     3721 taacatgtct catcaaaaag aaatctgccg ggtttccaat aacaccgccg atatcccgtc
     3781 caaaggccaa tccgaatcca ccaaataccc acataatcgt tgttatccca acggagagaa
     3841 aagattgaga catgatcgtt agtatattat tcttccgtgc taatccgccg taaaaaaaag
     3901 ctagaccagg agtcatcagg caaaccattg ctgtacataa aatcatgaat gctgtatctc
     3961 ctgtattgat aggtaatgta ttcatttttt tcgcctcctt tctacgatta cgagtataaa
     4021 agaaggtgta gttgtttttt tcttctgttt ttttgaatta atacttgcat ttataaaaga
     4081 cagaca
//
LOCUS       DAJSMI010000187         4048 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJSMI010000187 DAJSMI010000000
VERSION     DAJSMI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.191"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            69..848
                     /locus_tag="ON430_002526"
     CDS             69..848
                     /locus_tag="ON430_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR3220410.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            841..1158
                     /locus_tag="ON430_002527"
     CDS             841..1158
                     /locus_tag="ON430_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR3220411.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            1402..3012
                     /locus_tag="ON430_002528"
     CDS             1402..3012
                     /locus_tag="ON430_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3220412.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            3169..4017
                     /locus_tag="ON430_002529"
     CDS             3169..4017
                     /locus_tag="ON430_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3220413.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         1178 a          706 c          827 g         1337 t
ORIGIN      
        1 tacaatttag cgaatttatg ttacaatgag tccgctgttt atttgttgta attaaaggag
       61 atattttaat gaacaaaaag aaaagtgcgc catggcttca agctttgcat gcagtgatgc
      121 cactttgtat aagctatata ccagtaggat tagcttgcgg agtattgttg caaaaagttg
      181 gatttgatcc tctcttatca ggactgttat cgattctggt cttttccggt ggcgcgcagt
      241 ttttagttgc atctatgctt acgacgcaag catcatttgc aacgacatta ttgatggttt
      301 tctttttaga attacgctat acattattag gagcaagtct tgccggtttt atgaaaaaag
      361 aaaagcggac gtttttggct gtcttttctc aatcactgaa tgatgaaaac tatgcggtga
      421 actacttaaa gttttcaact gacccttcct ggaataaaag aaaggcttta tatgtcaatt
      481 ggttctcaat gactgcatgg gccggtagca atatgatcgg taattttttc ggctcggtga
      541 ttcgagtaga tgctgacctt gtccattttg ctttgacggc catgttcatt ttcatgttta
      601 tcatgcagat gaaaagcgca ctactgatct ttacaggttt attctcaggc gtattagggg
      661 tcgtattcat ggtattattc caaaatactt tcggattgat tgcagcaaca gtcattgctt
      721 catttgccgg atttatgctg gagaaggcat tcaaaaaaga aaaagctaag aaactaagag
      781 accgcggtaa agatgtcaat gagccgctct tcaacttccc ggaacaggag gccaatcacc
      841 atgactaaac tatatttgct acttttagtt gcttgtctgt tcatcgtggc atatatcccg
      901 cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc catcctggtt cagtgactgg
      961 atgaaatatg taccggtggc attatttgcg gcgttagctt ttaaagatgt tttcattact
     1021 catgaacatc tggatattgc ttggaatatc aaaatagctg caatgattct agtagcaggc
     1081 gtggcttaca aaacacgttc gatggcgttg tcagttttga caggacttgc gtctgtcttt
     1141 ttgctgtcta tgctttaaac tgttttattt tattaaaaac agagattgcg acataagttg
     1201 ttcaaactga tctatccgaa ctgcatttgc aacagtcaat tctggatgca agtttattcg
     1261 tagggataaa aaagattagt gttggcttat gatgcaagct cttttttatt ttaggaatta
     1321 gagagggatt tgtccttcta tgattttttg ctataatagg gaagatgttt attttatgaa
     1381 agaatagttg agggaattta aatgaataaa aaattgatgc aaggaacatt ttggctaacc
     1441 tttgctaatc ttctatgtaa agttttaggg gtagtctatt tgattccctg gctaagtatg
     1501 atggggaaca atcaagatgg tatgcttgct acgacattgt ataatgttgg ctatcttcct
     1561 tatggattgt tcctgatgct tggtacagtc ggatttccaa atgcgattgc aaaaaaagta
     1621 gctgtagcca caaaagaagg agataacgct gcttgtcggc tgatttttag aagtacgatc
     1681 aatatcatgt ttgtcatcgg tatcgcctct gctgcactga tgtatttatt tgctccttta
     1741 ctagccggaa tcagtccgat tgctaacgtt aataatggga tactagctat cagaagttta
     1801 tgcccatctt tgattgctat cccaattctt agtgcaatga gaggatattt ccaaggaaaa
     1861 aatgatctgc ggccttatgg aacttctttg attatcgaac aggctgtgcg agtgatcgtt
     1921 atcttggctg gtacttatta cttgcgcgta ttgactgacg gaaccatatt agaagctgta
     1981 ttgatcagta cagtcgcttc tttcttcggt gggttagctg cgattatcca tatgtatatc
     2041 gtcggacgca gaaaagactt cttcgaactt aaagattttt tgatttccag ccgttatttc
     2101 gaaagagaaa accgatctgc ctctgtctct atcattcggg aaacactacc gtttattttt
     2161 gtcggctcag tcattactat cgttcaaatg atcgaccaag taacgatgaa accactgctt
     2221 cactttttcc gtccagagat cgcagatcag caactggaat tcttattcag ccgtgcttct
     2281 gtcaatccaa ataaattgac attgatcctg atctccatgg taggaacagt agctataagc
     2341 agcttgccaa tattaagcac gatgaagaaa aaagatcgga tacaaatcga aaaaacagta
     2401 ggagatagtt tttcgattgc gttattgatt cttcttcctt cattgactgg aatgtcttta
     2461 ttagctggac cactatacac attgtttttt ggctatgatc ctgaaagtgt cggatatttc
     2521 caaatggctt tgctagcttc tttgtttttc tctttattca cgatcctatc tacgatgctt
     2581 caatctttga atcaccatcg ttttgcgatc cgattaactg tagaagcaat tatcctgaaa
     2641 gtcatcttcc aagtaatcgg attaggatta gttggcggat acgggatgag cttatcaaat
     2701 ggcttagcat tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa agaataccga
     2761 atagcacctt tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc caccctgatt
     2821 atgcttgcag tatgttttgc cgttttcttt ttgctgaatc agcgattgtc aatggaatca
     2881 aaaagctatg caatgattta ttgtgtggct gtaggaagta ttggcggctt agtattttta
     2941 ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca tcatagcaga
     3001 agcaaagaat aggttaacta atcgtttttt gctgtcactt gaaaaacggc aactcgtatg
     3061 attacgcttg tcgtttttct attttccaat tttagtttca tatctaatct tacttatgat
     3121 atagttataa ggtctggaaa aattctttga aacgaggaaa ttaaacacat gaaacttgca
     3181 gtagttacag atagttcggc atatctgccg gaacgtatca agaaccatcc cgacttattc
     3241 gttattccaa tccctttgat tatggatggg aaaatttata acgaaggaat cgatatcgaa
     3301 gcggatgaat attatggact tttaaaaaac agtaaagatt ttcctactac ttcacaacct
     3361 gtcgtagggg aagttttggg tttatatgag gaattgaaag agaaagggta tgacacgatt
     3421 ttaagtatcc atctttcttc agggatttct ggctttgtca acacattatt tgcgatgaaa
     3481 gatgacatca gcggagtaac ggtcattcct tatgactcta agatcactag tatgccgatg
     3541 ggacacatgg tagaagctgc cttagactta aatgatgaag gtcagtccct tgatgaaata
     3601 cttgcacata tcgatcgaat ccgagataat acctatgctt atctgattgt agatgatttg
     3661 aacaatttag tacgaggagg acgtttgaca aatggtgcag cgctgattgg cagcttatta
     3721 aaaatcaaac cgatcctgac attttctgaa gggaaaattg ttttattcga aaaaatccgt
     3781 tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg gtagaagaaa taacgaaata
     3841 caaccgccag tcaaacttta cgtgatacat gcaaataatt tagaagtagc gattgcggag
     3901 aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg aaattggtca tttcggacca
     3961 gtcattggta cacatttagg tgaaaaagcc atcggcattg ccatttcagc gcaataattc
     4021 cttttattca aaaaacatac caaaaata
//
LOCUS       DAJSMI010000188         3925 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJSMI010000188 DAJSMI010000000
VERSION     DAJSMI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.192"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(573..1130)
                     /locus_tag="ON430_002530"
     CDS             complement(573..1130)
                     /locus_tag="ON430_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220414.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ON430_002531"
     CDS             complement(1309..1920)
                     /locus_tag="ON430_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220415.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ON430_002532"
     CDS             complement(2033..2290)
                     /locus_tag="ON430_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220416.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ON430_002533"
     CDS             complement(2887..3165)
                     /locus_tag="ON430_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220417.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ON430_002534"
     CDS             complement(3199..3450)
                     /locus_tag="ON430_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220418.1"
                     /translation="MKEKPVNKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgctgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttacaggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DAJSMI010000189         3892 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJSMI010000189 DAJSMI010000000
VERSION     DAJSMI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.193"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..546
                     /locus_tag="ON430_002535"
     CDS             <1..546
                     /locus_tag="ON430_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220419.1"
                     /translation="VWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTAS
                     INEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGK
                     TVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGA
                     IKWRRNSSYGSDYTFAITYTI"
     gene            563..712
                     /locus_tag="ON430_002536"
     CDS             563..712
                     /locus_tag="ON430_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220420.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            709..1155
                     /locus_tag="ON430_002537"
     CDS             709..1155
                     /locus_tag="ON430_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220421.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1157..1294
                     /locus_tag="ON430_002538"
     CDS             1157..1294
                     /locus_tag="ON430_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR3220422.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1332..1625
                     /locus_tag="ON430_002539"
     CDS             1332..1625
                     /locus_tag="ON430_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220423.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            1622..1846
                     /locus_tag="ON430_002540"
     CDS             1622..1846
                     /locus_tag="ON430_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3220424.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            1843..2868
                     /locus_tag="ON430_002541"
     CDS             1843..2868
                     /locus_tag="ON430_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3220425.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            3058..3131
                     /locus_tag="ON430_002542"
     tRNA            3058..3131
                     /locus_tag="ON430_002542"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3092..3094,aa:Ile,seq:cat)
BASE COUNT         1346 a          653 c          779 g         1114 t
ORIGIN      
        1 gtttgggatc tagcaccaga atatgtcgaa gataatttgg ccacagatat taaaatttct
       61 gaaattactg gtaaagcaaa caattatacc gatgggaaag tatcggagat taattcgcag
      121 ttgactgctt caattaatga agtagacacc acagctaagg atgctcaaac aaaagcgaat
      181 gctaatgcga ctgctataga tgaattagac aataagatcg atgaacgcat taatgataca
      241 gctactacca cattaacagt tacaaacggg aataccggat cagcaaagct ttatcgtgaa
      301 ggaaaaacag tttctatata ttttgtggct ttaaacggaa aaagcagtgg tggaaatgat
      361 tcaacgatac taacaattcc agaaggctat cggccaccaa ttagttttga gcaactggtt
      421 ggctcgatag accgttctac tttgaacagt gctcagttat ctattggtgc agatggagcc
      481 attaaatggc gaagaaactc aagttatgga tcggattata cctttgcaat tacttacacg
      541 atttagaaaa gcgtgaatcg atatgaaggc agcatataga ccaattgaac cttacggatt
      601 cgagcaaatc attgtgaatg atgaagaaca tttaccggaa gaatgcacag aagtcgaacc
      661 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
      721 tggaaatatg gacgtactgg cggagagtac gcaggaaaag tattggacga catgcttgta
      781 tccgttcctt acacagatca gccaccgctt gaaggaattc gtgctgatgg cgaaccgtta
      841 acaatcgctg atcaaatgtt tgatcctaaa ttgaatcaat ggattatttt agcgaacgca
      901 ctagatcaca acgatttaaa caatctcaaa gcaatgtatg agtcgttaga aaatgagaac
      961 ggcgatttaa aacagatcaa tgccaaactc atgctaagcg atgtagcaat taaacaggaa
     1021 aatactgcat tgaaagaaaa agcggatagt ttagcacaaa tcaattcaaa aatgatgctt
     1081 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1141 aagggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1201 tttcactaac gaacaagtaa tggttttcgt tccgttgtgc attactgaag aaaaagcaga
     1261 taaaatcatt agcaaagaag agagcgcatc ttaattgatg tgctttttat tttgattcaa
     1321 ggagttgtca catgattaat ttaggggaat ggggagcgat agcaggatca ataaccgcta
     1381 tcgtttcttt gattttatta gtaataaaac caattactgc atctttctcg aagattactg
     1441 agactctttc aaaagtaagt cacaatttag atttgctgac taaagattta gaatcgagca
     1501 aatcagatcg attgatgatt catgaagaac taaagaaaca cgatgaaaga ttagatacac
     1561 atgcagaaaa attggtagaa cacacgcaac aaattaaaac tttatttagg gagagaagaa
     1621 aatgaataat aaaacgttcg aagtactaaa atggttcgca ctggtaatta ttcccgcact
     1681 agctactttc gtggggttag ttggtaaagc gctcaattgg cagtacacag atatctgtgt
     1741 tgtcatcatt actggttttg gcgcgttttt agggagtgtg ttgggtgtat caaatcgaac
     1801 ctacaaaatg ttctcggctg aaagcgaaga aggaggaaac aaatgaaaaa gaaaattact
     1861 attactgcga tgagcctgtt aacggctctt tttttattac ccattaatac gtttgcttat
     1921 actattaatg acgagtataa tttagcgccg aatcaaggag actccagatt agcaattcct
     1981 aacaaaatta ttttgcacga aactggaata gatgcaccag caagaaacgt agccgccaac
     2041 atgaaaaata attataacgg aagcaattct tatactacag atgttattgg tgacggtggg
     2101 attgtttacc gtgtgggtga gcaaggatat gtttcgtggg gagctggtaa cgctaatcct
     2161 tatgcgcctg tacagattga attacagcgc acatatgata aagcattgtt tgaaaaaaac
     2221 tatcgagctt atattgaata tacaagagat agtgcaaaaa aatatggaat tccattgact
     2281 cttgatcaag gaacttcttt atttacaaaa ggaatcattt ctcatttgtg ggtgacaaat
     2341 tatgtttggg ggaaccacac agatccatat ggttacttat cgcaaatggg agttagcaaa
     2401 gaaaagcttg cttatgattt agctcatgga tttaccgatg aaaatccaac aacttctgaa
     2461 aacaagcctg tcattgatcc aacacgagct ggtgcagcta atcctacgct gacagatgga
     2521 acaaatcacg cccacattga tcagtttggg gaaatcgaaa acgcaaactt gcatgtggct
     2581 ggatggcaca ttgctaacta taaatacgag tatattttca ttatggacta caatactggg
     2641 aaagaattag ctcgagtaag agctgatgga atttatagac cagatgtaaa ccaagcttat
     2701 aatacttctg gaaatgttgg ttatcatgta tctttcaata tgcgtaattt tcctagtaag
     2761 aaagtctatg taatgatgcg ggcaacgaat gatccagagg gaaacactaa aggcggtgcg
     2821 caagatttcc atgacaaacg ttggtattta aatattccta aacgataaaa atagctcctc
     2881 gttgaggagc agtacataac tatattgaca actataaaaa ttattcgata aaatagtgat
     2941 gttatcgcat atcttcacta tcacccataa atagtcacac tccaagctat gcgataacag
     3001 gtttgttgcc acacattcta ctggttgatt gtttatggct ttatgtggca acaaccagta
     3061 cccttagctc agttggtcag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
     3121 tacagggtac attaacgtag ccatttgaat cgttctgtgt tagaattttt ttgaagagta
     3181 ttatacaagc taaagctttt cttcattgcc actcaaatga gtggcttttt tatgtatcct
     3241 tttatggatt aatgaaagga tgtttcacat agttatactt ctgtatattt gaaaagtttt
     3301 actttgattt ttaaatagaa agacatttgg gttaaattgt gagataataa taaagaagag
     3361 tttaaagcgc accccaaacc acttccccat aagtgtgtta cgctttaaac tcttttatat
     3421 ttgaagccat taaaaagcat accatatttt tgaaaaaaag tgagaaaaaa ggcttacaat
     3481 tggagtggta gttaattagt gacttatttt tgattttata gcactgatac tataaaatat
     3541 agatatcatc atattacaca atcttaatac taacttaaaa aatatctcct ttcacaagta
     3601 tggtgataaa attcgttccg ggctaccttt ttaggtagcc tactttaatc tttatacctt
     3661 tctggatcaa cgaaagtata ctttatatag tcataacgcc gatgatcgct acgtgcgtcc
     3721 ggcacgtcag tcatgaatcg gctacactag actagacaga aaaaataagg tgtgtagaat
     3781 aataaaaaac acactggagg atttttcatg tcaagacgtc aacgaagaac ctattcaaaa
     3841 gaattcaaac aacaaatcgt cgatctctat ctcgctggta agcctcgcgc ag
//
LOCUS       DAJSMI010000190         3877 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJSMI010000190 DAJSMI010000000
VERSION     DAJSMI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.194"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(717..3866)
                     /locus_tag="ON430_002543"
     CDS             complement(717..3866)
                     /locus_tag="ON430_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCR3220426.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          636 g         1362 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccct
//
LOCUS       DAJSMI010000191         3817 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJSMI010000191 DAJSMI010000000
VERSION     DAJSMI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.195"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..667
                     /locus_tag="ON430_002544"
     CDS             <1..667
                     /locus_tag="ON430_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCR3220427.1"
                     /translation="LGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLELLGT
                     SFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMIFAA
                     FNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQVLF
                     IGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKYYPG
                     KGQ"
     gene            669..1613
                     /locus_tag="ON430_002545"
     CDS             669..1613
                     /locus_tag="ON430_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCR3220428.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1812..2456
                     /gene="pcp"
                     /locus_tag="ON430_002546"
     CDS             1812..2456
                     /gene="pcp"
                     /locus_tag="ON430_002546"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCR3220429.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2535..3812
                     /locus_tag="ON430_002547"
     CDS             2535..3812
                     /locus_tag="ON430_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220430.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         1215 a          670 c          850 g         1082 t
ORIGIN      
        1 tttaggcgtc ttgattgtta ttctcgggtt tgcatttaaa ttagactcga tattgatcgt
       61 aatggttgct ttagttgtta cagctttgac tggcggtctt ggcattcaag gaatgttaga
      121 acttttaggt acaagttttg taaacaatcg cgggatggcg atttttatca tcattatgtt
      181 ggcaacagga acgcttgaaa gaaatggtct gaaagaatca gcggcttcct tgatcaagcg
      241 attcaagaaa gtatctgctg gtatgattgt agatatttat gggatctttc gaatgatctt
      301 tgcggctttt aacgtgagtt ttggtggagt tgctggattt gttcgtccaa tcattttacc
      361 gatggcattg gggaccatcg aatcaaaagg gttagaagtc catcctaagc atgaagaaga
      421 actaaaaggt atggcttcag ctatggaaaa tgtctgctgg ttctttggcc aagtactatt
      481 catcggagga gcaggggcgt tgctagtcca atcaacactg aaagatctag gatatgacgt
      541 aacattggga cagcttgctt tagtggaagt tccagtggct atcacggctt tagttgtggc
      601 aagtgtctac ttctatctta aagaaaagaa attagcgaag aaatattatc caggaaaggg
      661 gcaataagat ggactttttt acaaatcctg atattttatt aggcgataaa ttactggaga
      721 tcctttatat cgtgatggga ctcgtattga tttacactgg agtaagaaat ttaacagata
      781 aaaccaatcc tcatcgttat gggtcagcct ttttctggac ggtcttaggg gtagttattg
      841 caggaggaag atggcttccg gcaatcgtga acggtggctt gatctttgcg atgaccatac
      901 cagcaattgc taagaaagtc agcaaaggag aaagccggct tccatcaaaa gaatatatgg
      961 aaaaaatgtc agacaaacta ggaatgaaaa tttttattcc agctttgagt atcggtgtct
     1021 ttgcgatctt gtttgctcta tttaccaatt taggtgcttt agtcggcgtg ggcgtcggtg
     1081 tttttgcagc catgctaatc ttgatgttct tttctcgaga taatcgacca agtacatttt
     1141 tagatgatgc agcagatatg ctgggcacag tagggccgtt aagcatgttg ccaatgcttt
     1201 tagcttctct aggtgcggta tttacaagcg ctggagttgg gacagtgatt tccaatgcag
     1261 taggtacgat cgttccagaa ggaaatgtga acatcggcat catcatttac gctcttggca
     1321 tggtgatatt tacggtaatc atggggaatg catttgcggc catcaccgtg atgacggttg
     1381 ggatcggtgc accattcgta tttagtcatg gagcgaatcc agcgttaata ggaatggttg
     1441 cattgacttg cggtttttgt gggaccctgc tgacaccgat ggctgctaac ttcaatatcg
     1501 tgcctgtagc gatgctggag atgaaagaca aatacggagt gatcaaaaac cagttgttta
     1561 ttgcgttatt tatgctagtt ttccaaattg cttatatgat tctatttaaa taatagaaga
     1621 gaggggttgt gacagaataa ttgtcgcaac ctcttctaac attgtagtgg aacactattt
     1681 ttttataaga atatattaag tttgatctgt tatgttattt aaactattgt ttcctttatt
     1741 tacgctgttc aaggaaaatg gtatgataaa agataaccat atgttaaatc aatcggacgg
     1801 aggaacaaca gatgaaagta ctagtaacag gttttgatcc atttggtggg gacaaagtga
     1861 atccagccta tgaagcagta aaaaaaatgc cagatgaaat cagcggtgct gaaatcatca
     1921 aagtggaagt accgaccgta ttcgaaaaaa gcagtcaagt agtcaaagaa gccatccaac
     1981 agcaccaacc agatgtcgtg atctgtgtcg gacaagcagg tgggcggtca gcagtctcgt
     2041 tcgaacgtgt cgcaatcaat ctagcagaag caagaatccc tgataatgaa ggaaatcagc
     2101 cattcgatac ggctttagaa gaaaacggtc cagccgctta ctttacctca ttgccaatca
     2161 aagcaatgac aaaaaatgta caagatcatg gtctaccagc gtacatctct tatacagcag
     2221 gaacatttgt ctgcaatgat atcatgtatc gtttgcttca tttgatcgaa acacaattcc
     2281 cggcaattcg aggtgggttc atccacgtgc cattctcacc cgatcaagtg atcgatcgtc
     2341 cagtgggtac gccttctatg tctttagaag acattgcttc ttctttgact tatgcagttg
     2401 aagcagcagt agaaaataaa gaagatattt ccggaaatgc cggaacaact cactaattta
     2461 gcaagaaaca ctggctggtt gctaacgtta tgttagtagc cagctttttg taattagaaa
     2521 ggaaagaaac agccgtggaa gaatggcaat ccgtctttga agaatggttt ccaaaagaaa
     2581 tcagcaaaag ctatcccatc aaaatttcta aacaatacac aagcagtcaa cgttgggaga
     2641 tctatgcaaa attaacgaaa aaacagcggg aactagtcga taagcaccgc cgatatctaa
     2701 tcagctctcg ttttatggaa gaacattatt tagctgcaac agattgggtg ttttctgact
     2761 tcaagatcaa tccgtttttt cgaacaaaac gaagccagca aaaattgtat tgtgagtgtg
     2821 gtagagaatt aaaagtgcaa tacatcgtta aatcgccaaa aaccggaaag atcctaaaac
     2881 taggcatcaa ccactttgcc gatcacctgc atgtctctcc gacagtggca gcttcgatcc
     2941 atcagggaat gacaaaagtt gatctggcct tagacgaatt attatggctt aagcagaaga
     3001 atatcgattt tcctgaagga ctatggcaaa aatactgttt tgtcttgtac caaaatcgtc
     3061 ggatgaaaca gccatattta ccggatataa aattagctca gcggctcgca gaatttcgcc
     3121 aagtagaaat gccgatttac atagcggatt atcaagcatt ggaaaatgaa atcaagaaaa
     3181 taagtgaaca catcaatggg cagtcaaaaa agcgacagat aaaaaaagaa ctatttgacg
     3241 actttgcaga agaattagta aaggatgtag aagaattctt gatcaactat cgcgcatttt
     3301 tacgtaaaga ttggcaatcc atcgtatatg aagaagtgcc agtacatcct aatgcttatt
     3361 ttgaaacatt tatttccgtg ttgagaaaga caaaacgtca acgaacacca gaagtcactg
     3421 ctcagatgga atattttgcg aaaaaccaac gattcatcca accaaaaatc tatctattta
     3481 tttggaagca atattgtcgc tatggtttta cagaagggtt cttcgacagc attcctcgga
     3541 tagtcagaaa tggtttttta aaagtattgc gtaaagaacg agaagctata caatctgctg
     3601 ataagaaaga ccgtacagtc tcaaaagaaa aatggcaact agtagtgaaa gatatccaat
     3661 ctggaaatgt gcaagagaca atcgacaaat ggaaaggaaa acattatcgt tttactgaag
     3721 ctcagaaaca agcgttggaa tattatcaga agttagaaga atcactgcgt ttcaatgatg
     3781 aggcaagaaa gtatctgaag gaattactat aaaaaat
//
LOCUS       DAJSMI010000192         3804 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJSMI010000192 DAJSMI010000000
VERSION     DAJSMI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.196"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            4..2376
                     /locus_tag="ON430_002548"
     CDS             4..2376
                     /locus_tag="ON430_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220431.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..>3804
                     /locus_tag="ON430_002549"
     CDS             2437..>3804
                     /locus_tag="ON430_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR3220432.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERSNGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPE
                     VKKDWN"
BASE COUNT         1484 a          605 c          822 g          893 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
      661 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agagttgatc
      781 gatcaactta aaacgttgga taaccaatac gctgcagaat caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgttacga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgag
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcatgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatt gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacaa ttgaagcctg aagactatcc gaaaatcttc
     1501 ccgaaccgcg tctttgattt tcaattggat aaaacagggc agacagaact tcaagttggg
     1561 attcaagcgg tagccaaaaa tattggaatt caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtacgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaattttc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaatg agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtttcgat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtc gcaaaagaag aaaagatcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagcttgtga acgctcgaat
     3661 ggtacgtacg attttgatcg tttttatcaa ggaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa agcatgaaca tgaatttgta
     3781 cctgaagtga aaaaggattg gaac
//
LOCUS       DAJSMI010000193         3797 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJSMI010000193 DAJSMI010000000
VERSION     DAJSMI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.197"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            895..1209
                     /locus_tag="ON430_002550"
     CDS             895..1209
                     /locus_tag="ON430_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220433.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(1262..2035)
                     /locus_tag="ON430_002551"
     CDS             complement(1262..2035)
                     /locus_tag="ON430_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220434.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            2140..2385
                     /locus_tag="ON430_002552"
     CDS             2140..2385
                     /locus_tag="ON430_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3220435.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            2541..3392
                     /locus_tag="ON430_002553"
     CDS             2541..3392
                     /locus_tag="ON430_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HCR3220436.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            3425..>3797
                     /locus_tag="ON430_002554"
     CDS             3425..>3797
                     /locus_tag="ON430_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220437.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNP"
BASE COUNT         1421 a          517 c          645 g         1214 t
ORIGIN      
        1 taaagaaaag agaaaagtaa ttaaacgcaa aaaaatgtag aacttaaaaa atataaagag
       61 gaacaaccgt taaaattagg aatgatgttg tgtaaacatt ataattgcta tgatataaat
      121 gaagtgttaa agaaagcaaa tttaaaccaa ttttgtgaac agaaaagcct tagtcactat
      181 gaccaaaact acggacgaaa ctatgaccaa aagtaggtac taaattatga ccgaaaatag
      241 gtccaaaatg gattttttta tgatatcaaa aattatgaaa tgattttgaa agaaaaccag
      301 ttggcacagc caacttagcc accttcctgt aggaaggtgc tatcagtttt cccttatgct
      361 cttttctttc ttttttgttt catatgtcaa atgccatttt gaaatgtacc atcttaagaa
      421 atttatcctt aaaaacaatt gcgttattgg ataaaaagat gctacattat cttaaaaaag
      481 gaatcaaata tgatgaattt agtggctagc atatcgtgtg attaagatac tttatagtgt
      541 aagttgaagg agaaaaaata tttaaaatat acttcccatt tcagtgggaa gtaaaattaa
      601 tcttgttgtt tctactagta atgtatcttt ttggaaacta tgtaacgatg taaacaagat
      661 ttttttcaga agaaaaaacg ataaaagttg tgataaacga tttaaaaaat aggagaaaat
      721 atatcaagcg catggtatga aacgcacatt taaaaagatt cgactaacta aaaaaataag
      781 tttacaaagc attgttgtgt attacaagtg atctatacac tgaaaaatga tagagaaaat
      841 ctgatacgtc cgcaacaatg gacaattgaa aatgaaagtg aggaaattgg aagaatggct
      901 gatgaaaata aagttgttcc gcttgaaaca ctagtgataa ataaggaaga gtttgagcaa
      961 ctttttcttg atttttttgt tcaatatgac aggatttatg attttagttc gtcactagat
     1021 ttatttgaat tagaggcaga aataatagaa aacagtcgag aggagaatgg taaaatcatc
     1081 atttcagtag aaaaattcaa tgaattattc tcagaaacat ttgaaataga tacagattat
     1141 tatcaaccac aagaagtttt ccaatcattt agaaatcaga ttattcgctc aggtaagacc
     1201 gtaaactgaa aaacaataaa agaaacgttg aaagaataat tattccttca acgtttcttt
     1261 tttagaatgt tttaatgacc aaagcagttt cattgtcagg cttaggtaat tcatgaaatc
     1321 ccgtatttat agtgctcaat tgatcaacta accatgtttt actttttgaa ttgtagataa
     1381 atctaaaatt aatatcgtta ttatcgtgtt gaaggggcgt ctccaaataa tctgctgtat
     1441 attcatagtc aacgtaacct gctaatacaa aagagagtat ccctttattt aatgttagat
     1501 ttaatggata atcatacgac tttatttttt tcaacttcat attgtacata tcttcagcca
     1561 taaaagttga gttcgtgatt tcatcttggg tctcattaaa aaagttatcc gaataatcgc
     1621 ttatttttga aaagtcttta ttggcaccag cctcaaaaaa ttgttcaact ttttcagtat
     1681 aaagttgtgc taacgctcct ttttcaatat taactttaat attttcatgt ctatcaattt
     1741 gtgtgctgtt cagtaaggga agatcagctt ttagttttat atcgtcttga tttctttcaa
     1801 tttcaattgg caaaatcttt tcagtagatt cagtttctga taaaggaaca tctagttttt
     1861 tatcatgata ataagcttca aatccatctg gaaccggaaa aaaatgtatt gggtaattct
     1921 tttctgaggt tgttgtacta gtttccgtac ttgtagaact tgtgtcagaa gtggtacacc
     1981 cgcttatagt tccgatgagt aaagcgcaag ttagaatgat tgcaaatttc ttcaaaaaaa
     2041 ttcctcctta aaattgaata tggactaata ctagtcccat tgtagcatac cgaatcttta
     2101 agattaataa gaaaaaggac taattttagt ccaaaaggtg tgctattctt gaaagaggaa
     2161 aaaattattg ataaagaaaa tgtaatcggt agcaatatta gacgagtccg attagaaaaa
     2221 ggaattggtc aaaccgagct gattagagac ctacagcttc gtaaaattgc tataactagg
     2281 gaaacgttgg ttaagattga aggtggccgc caacacatta agttggatca attgaaagcc
     2341 atcaaagata ttctacaggt gtcttatgaa gatttgcttc aatgactact tgtagtcatt
     2401 tcgtttgaaa ttattatgtt acacccttat gtgtcacgaa gaaatactgg tataataatg
     2461 aatgggatat gtaacgatag ggtatactgt tatgttgcct aataaattgt ggatcaaaat
     2521 acaagaatgg agggatatcc ttgtataagg catatgaatt ggattttaca aatgctgact
     2581 taaatgcatt ctcctcaagc tcgcaatcat atacacatgt agtaaatcaa atagatcaca
     2641 accaaaaatc tattaacaag agaatagaaa atctatttga aagtgaaaat gatttagttg
     2701 attattattc taaggacagc aaaattctag atgcagacga aattattaaa gattggtttc
     2761 caactataaa agcagatatt tttatatctc actctcatgc tgatgaaaaa ttagctatac
     2821 gttttgcatc atggctattt gaaaattttg gtcttactgc atttattgat tcttcggttt
     2881 ggggatacag ttctgattta ttaaaaaaaa tagaccaaaa atattgctat aaggaacaaa
     2941 caaaaacata tgattatgat aaaagaaatg taacaactag tcatgttcat atgatgcttt
     3001 ctacagcttt aaataatatg attgatagta cagaatgtct gttttttctt aatacaccta
     3061 attcaattag tttgagcaat gaaatcacta atgaacagaa atttacgtat tcgccatggc
     3121 tatatagcga actgacgaca gcttcaatag tggaaaaaaa gaatccacga ttggaaagta
     3181 atcctcaaat gagcaccgag gatgttagaa gcattataaa gcactattct tcgcaagaaa
     3241 atctgcaaat aaaatattct ttaaatttaa gtgaattaac ttatttacga aaagctgatt
     3301 tgaaaaaatg ggctttagat gctgtggaat gcactaaagg aagtaataca ttagatattt
     3361 tatataaaaa caccattttc actaatctct aatcaattta ctgaatcgaa aggaaccggg
     3421 tagaatggat aagaaagttt taataaagag gttctctgaa gctattgaaa aaggaaatgc
     3481 agcaatattt gcaggtgcag gattatcgat gtcacaagga tatgtaagtt ggccggaact
     3541 acttaatgat cctgcaacag aaatcgggtt ggattctaaa aaagaaacag acttagttac
     3601 tctggctcaa tattataaaa atgaaaatgg tggatcaaga ggaattttga atcagatact
     3661 tatggataat tttggtgaag aattagaaat atctgaaaat catcgaatac tagcaagttt
     3721 acctattgaa acttattgga caactaatta tgatcatcta atagaaaaat caatccgaga
     3781 agcttataaa aaccctc
//
LOCUS       DAJSMI010000194         3795 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJSMI010000194 DAJSMI010000000
VERSION     DAJSMI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.198"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..404
                     /locus_tag="ON430_002555"
     CDS             <1..404
                     /locus_tag="ON430_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220438.1"
                     /translation="EEEDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTED
                     IRKIIEYLSENTQYLIAECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLE
                     NVEPIDQFKKAFEIKELPEITMHNWLKGEES"
     gene            401..619
                     /locus_tag="ON430_002556"
     CDS             401..619
                     /locus_tag="ON430_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220439.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            606..3233
                     /locus_tag="ON430_002557"
     CDS             606..3233
                     /locus_tag="ON430_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220440.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            3248..3466
                     /locus_tag="ON430_002558"
     CDS             3248..3466
                     /locus_tag="ON430_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220441.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         1453 a          496 c          763 g         1083 t
ORIGIN      
        1 tggaagaaga ggatattaat ataaagagga tttacagtaa gattagtgtt ctttatgacg
       61 caaataacga agatggtcga aaattatttc aagtgtatca ctatttgaga aacgctaatg
      121 tagatacaga agatatcaga aaaatcattg aatatttatc tgaaaatact cagtatctta
      181 ttgctgagtg tattataaaa caacatgaag catgcttttt tcaaagaaaa actgaagaag
      241 ggttatacga ttatgtaaca tattttttga atggacttca gaaaagatat aaaagtcaat
      301 ttttagaaaa tgtagagcct atagatcagt tcaaaaaagc tttcgaaatc aaagagttac
      361 ctgaaattac tatgcataat tggttgaaag gagaagaatc atgattaaaa atcgcttaat
      421 agatcaaatg tggtgtcttg atcaaaatac agatactctc gaattaatcg agagtatctg
      481 cttcaacaca gtagttctcg atttaagagc tgaaaatgac aattgtgtaa ctcatatcat
      541 aattaatcaa aaaatggctc agcagctctc tgagagccta agaaaatggg ctgaaggaga
      601 aaactatgaa agcaattaaa gaaaaaatcg ttagtagaaa aataaactgg ttagcactca
      661 gtgtacttgt tttaatgatt agcgttgttt ttacaagtaa tattgttgaa gctagcattt
      721 tcaattggtt tgatgacaca aaaagtcaaa cagagttttt gaataattct ctttatagac
      781 cttacctaaa aaaatacaat ggagatttcc ttagtcagtt cgggatttgg cttggatggg
      841 ctgtggtaaa aggcatgttt actgtgactg attcgattca aaatatgatt ccagatgtgt
      901 tggatttgtt caatttcatt gaaagtacag ggctaaataa tgtttatcaa tcagtcataa
      961 atacaattgt cgttggatta atgatccttt cattgatgtt tgttggctac aagatgatta
     1021 ctggaaaagg aacaatagat ttaagatctg ttggaatgaa tatagtgatg tctgtagcgt
     1081 taattctttt aatgccaaca atgatttcta gcggtattca atttgccaaa atattttaca
     1141 atgatgctac tacgattaca aattcagatg acggagtcgc ttggtcattg ataaaacaag
     1201 gagtaacaga tctggcatat attaataaaa cagatcaata ctcaagcatt gataaaacgg
     1261 aggatcgaaa taagttaaca cgaaaaaatt ttcaacagac agatttaaca caagtgttga
     1321 cggataaagt gattgataaa ttggaaaaag aaaatccagc tgcggataat cttcgctatg
     1381 aattagttga aaattctaat aatgaatttg ttgccacaaa gttttctgat aacttcttat
     1441 caacattttc tgatagcttg aaatcaggtt attatcgata tcaagcaaat ctttggggga
     1501 tttcgattgg gttgacagcg ttggcaatcg cctatgtgtt cagcgcgttt gtgatcatta
     1561 cagcaatatt agaacttgca tttaaacgtg tgttaggtgt tcttgttttt gcgacagata
     1621 ttgaaacagg acaacgttca aaagttgttt tatccgatat tttacaatgt tacttaacag
     1681 ttggatttca agggtttgga ctatctatgt ttgctatgtt tatcaatttt ttaaatgctg
     1741 gacaaggtat ctccacaaat atttttataa aaacaattgc ttatatctgt gctgtgtttg
     1801 tgctgataaa aggcagtgga acagtcatga gatactttgg tgttgatatt ggactcaaag
     1861 aaggctatgg gcaacttgct tctgcttttg gtatgggcgc aatgctgttt agaaaaggat
     1921 caactggatt taatcgtgct aaaggaagcg gaaatggaaa tggatcagac agtggagaag
     1981 gagaagatcg aaaaccagaa aagaatttcg gcgaaactct atcaaaaaaa gctggcaaaa
     2041 ctggaagagc tttgggatat gcccatgaac gtggattatc aggtttagct tcagatggag
     2101 ctacaatggc tagtgaaaga gcaataaaac catttaagag tatgcgtgat atggctaatg
     2161 ataccaagaa taagttcaaa gaaggtttag atgatggtac agtgtccgct attaataaaa
     2221 atagcaaacc tatgttagct aaaaataaag aagatgaaac tggaaaatat gctgattcca
     2281 tgccaacacg tctttcggat agaaaagatg gtgctaaggt tgaaaatgcc gatagaatta
     2341 tgtctagttc tgaacgtatg cgtgaagcaa tgaaaaataa tgctgaatcg aataataatc
     2401 ctgtaagtgc aattcagcaa aaagttcaac aagatattga agaacgtaaa aatgctatgc
     2461 acggtcaagc taagtctgct gaagaattaa ttaatcagaa gcgacaagaa gctaaataca
     2521 cgcctgaagc aatgaatcgt gaggaaatgt taaggaaacg tgtagaaggg cgtacaggag
     2581 ctaatacgga cgaaaaagaa gcgttgacca aagaaagaat ccaagaagct aaaaactcaa
     2641 ataccggatt agaaaaattg gtcaaagaaa atctacaaaa ttctacaagt agtcaaggtg
     2701 gccgatcagt agatgtccga gaaaatattc aaggttcatt gaatggtcat gtcggacgga
     2761 atgttgatgt aagagagaat ttacaaagct cttcaaatgg tcaaggtact aagacaattg
     2821 atgttcgaga aaatgtacaa aattctacaa gtagtcaagg gtttaaatca atggatgtaa
     2881 gagagaatgt acaaaattct acaagtagtc aagggtttaa atcaatggat gtaagagaga
     2941 atgtacaaag ttctacaagt agtcaaggat tcaaaacagt agatgttcgg gagaacgttc
     3001 aaaaagatag tggagaaatg aaagaaaaaa cacaaaaaat caatatcgtt gaagacagaa
     3061 agtcagcgaa aaaatttgat tcaaatcatg aaacagtgat tattgatagt gaaatccgtg
     3121 aaaatgataa gggatcaaaa ccaagacgaa gatttaccta tgaagacaac gagttattca
     3181 gagatacact taatgatcct aatccgcttt tcgataatct attaaaaaaa taaaaggggg
     3241 aattgtcatg aaattaattc taaagattag tacgcatgat ttcttagaac ttgacgaatt
     3301 atgtgaatca gtaaagaacg caacaaatga aaaaatcacg gtcactaatt atattgaact
     3361 gatgattcaa gaagaattaa agatgaaaca tgagataaaa aacaacttga aatattgtta
     3421 tctagaaaaa agtgaagaga taatccatga attaaatggc ttttaaaaat tgtatttgac
     3481 atgaatttac gacaaggaat tagtggggat taagcgatta ttctcttcca ctttagagaa
     3541 ataatgggtt aaaaagccag aatacgtaga gcaatattga caacctataa aggagctatt
     3601 gttgaaaatt gaagtaacaa aaagataaag agaggtttac atctatcaga ttaaaagtga
     3661 tagtacaccc atgaaacctg caaaagagtt gaacctaatg tatatcggtt taactctttt
     3721 tatttgcaaa ttaaaaaaat atcaccgaag tgacgaatga aactatgacc aaattaagca
     3781 agaaaatata tcttg
//
LOCUS       DAJSMI010000195         3686 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJSMI010000195 DAJSMI010000000
VERSION     DAJSMI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.199"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..408
                     /locus_tag="ON430_002559"
     CDS             <1..408
                     /locus_tag="ON430_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3220442.1"
                     /translation="FLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIFLAIDFILLFAL
                     LCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSGIILGVTWQFKG
                     LILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            401..2125
                     /locus_tag="ON430_002560"
     CDS             401..2125
                     /locus_tag="ON430_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3220443.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            2122..3573
                     /locus_tag="ON430_002561"
     CDS             2122..3573
                     /locus_tag="ON430_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3220444.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         1328 a          592 c          723 g         1043 t
ORIGIN      
        1 tttttaagag gcaatctgct atttagttta tttttatctg gtactgcttt attgagttac
       61 aatttatatt tatctgttca aatcaaaggg ctcatttttt tagctattga ttttatttta
      121 ctttttgcct tgctctgcct atttgctact tatcaataca gtatgatttt agatagtgaa
      181 tatacgatct ctgttcctaa tctgctaaag ctaagtttta tttcggtttt ttccagcttt
      241 tcgtcttttc taaaaataat tattggtagc ggtataattt taggcgtgac ttggcaattc
      301 aaaggattga tcctgtttgg tgtcattggt cttttgacgg tttggaatgg tacgatgaca
      361 acacattggc gtgaagaatt agataaacag ttggaaagtt atgaataagt ttcggattct
      421 attttcaaaa ggaaccttac taaatcgtct gttgcggaaa tacgcggtta tcatgatcgg
      481 tgtaacgatg actgctaccg ttatttttag tatccatact tgggaacaaa atcaaaaaca
      541 ggcagaaaac atgacaagtg atgccgttca aagcacaagc agaatgctga atgataaaac
      601 aaccttgagt aggatcatca aaaaccagct tgtagggaat tccgaaaaga tcgaaaatgt
      661 gacgacctac ttaacaaaac ccatagatca gtatctgatg tatgtatacg aacagcaaaa
      721 cagtaccgat gaattagttt ctttcccaaa tcaaatcaaa gatctctacg caaattatga
      781 agaactaagt gcgatatata tcgtgttgaa tcaactaccg gagtattatg aatcttctag
      841 agaaaacaaa ggtggggaaa taaaaaaggg gacaccaaaa ttactagacg ccttttatat
      901 caagttgcct atcacacaag gaggagctga gtcaggaagt atttttattg gttttcaaaa
      961 gaaagaacta gatatgattt tagaaaatct aacgtcattc aatggactct ctttatacat
     1021 gatttctgga acgacaaatc gactatacac attccatgat aataaaatag ctaaagaaac
     1081 gtttgaaaga caagaaatga tcatcacaga gagtctgaag aaaaattcac aattgccgat
     1141 tgacgatctg gaaaaaaaca attttgttca acaccaagaa ctgtctgatg attatcgtat
     1201 attagctgta ctagataaaa aaagtgtaaa aatcgaaacg ggaaaaaatc ttgccccgct
     1261 gatcattggt atcattattt tagacaatat actgcttatc tttctttata aaacattcaa
     1321 acgatattca cagcaagtat ctatcgtcat gcaggccatg gatcaaacag cagcagggaa
     1381 tttagagacg cggatcgaca taacaaacac ggaatatgaa ctgaaggaac tttcaatcgg
     1441 tatcaacgaa atgttggata gcatcaatca gtttgtagaa gacatctaca aattagaaat
     1501 caaacagcaa gacgcacata tgcgggcgct tcaagcacag atcaatccac actttttata
     1561 taatacatta gaatatatcc gaatgtatgc aatcagcgaa ggaagtgaag aattagcaga
     1621 tgttgtgtat gcgttttccg ctttgcttcg aaacaatacg aatcaagaga aaacgatcac
     1681 tttaaaagaa gaactggact tttgcgaaaa atatgtctat ttgtatcaaa tgcgttatcc
     1741 taatcgagta gcctatcatt ttatgatcga tcctgatttg gaaaagatcg aagtacctaa
     1801 atttgtgatc cagccattag ttgaaaatta ttttaaacac ggtattgatt ttactcgatt
     1861 cgataatgcg ctaagtgtca aagtcttgca ggaaggcaaa cgggtaagga tcatcatcaa
     1921 agataatgga aaagggatga ctgaaaaacg gctaaaacag attgaagaaa aactgtctca
     1981 tcctaaagtg gaacttcacg gttctatcgg tctgcaaaac gtgaatgaac gtttacgtgc
     2041 cagcttcggc agcagttatt atatgtcgct tgagaataat gagacaggtg gactgaccgt
     2101 ttctattaca ttcaaggagg gatgagcgtg tatcaagtgt tattggtaga tgatgaatac
     2161 atgattttga atgggttaaa aaagataatt gattggcaaa gtttaggctt tcaaatagta
     2221 gcaacagcag agaatgccaa agaaggacta gctgtacttg aacagcgaca gattgattta
     2281 gtagtaacgg atgtgacaat gccagaaata aatggactgg aatttattga agcggctcaa
     2341 aaagaacgtc ataattttga atttatgatt ttatcaggat accaagaatt cgattatttg
     2401 aaaggaggta tgcaattagg cgccgtgaat tatttgatga aacccgtgaa taagttcgaa
     2461 ttggttgaaa gcctcaagaa aatcaaaaca cggttagatc agcaaaatga acaaaaaaat
     2521 caacaagaaa tctatcaaga actgttattc agtcaatggt tgaatgaaga attggatgaa
     2581 ataagtgaag aagaaataat aggacaaata agtgagaagc agcataggat agtattgatt
     2641 caactggcaa gaactcatga tactcttatc aatagatggt tgcaagaacg gcaacaaatc
     2701 ttttactatc aacgaaatta cggagaccgt gtattgatca cgttgttgtt ggaggcagat
     2761 gaagttgatg gcttctgttg ttttgttcaa gaaaactttc ctgatcagga atggctgatc
     2821 agtatcgggg aagagacaga ttctttggac aaaatccctg aaacattcca acaagcgaaa
     2881 gacaatctac agttgcatca attttatgga gataaagacg agcatgtttt ttacgcagta
     2941 caagcagaaa caaaagatca aaccatcgat ttttcaagtt tcagacgcgt gctgcaaaat
     3001 aaacaattgg aagtcgctca aaaaatgatc actgattttt ttgaacagtt tcagttagca
     3061 gccatgatgc cggaagatat acgatattcc agttttttac tgtttatgga gattcaacgg
     3121 gaattgattg atctggaaga tgaggaatat ttacaaggaa tcgaaaaaat ccatcaggca
     3181 aaaacggtgc aagaacttca gcagctactt ctgtcattca tcaaaaagca tcagagacag
     3241 aaaaagtaca gtgacaatgt ggaaaaagta atcgtaatcc tgcaccagca ttatcaagaa
     3301 ccacttacac tcaaagaagt gtcagaaaac ctgcatttaa atgtcatgta tctaggacag
     3361 ctgttcaaaa aagaaacgaa aaaaagtttt tcagcttatt tgaaccattt acgtatggaa
     3421 aaagcgaaac aactgctttt gcacagtaat caaaacatca atgagatagc aagtgagatt
     3481 ggctacaaca acaccacgta tttctctaaa ttattcaaga aaatcgtcgg cagatcccct
     3541 aaagagtacc gagaaaacag tggagaatca taaaagaaaa agccaatgta atgaaataac
     3601 ttataaaaaa ttgtctagaa atctaatttt gggctgttct tattacttga ggcaaggcag
     3661 ctttttcatg acctctcgat aaacgg
//
LOCUS       DAJSMI010000196         3620 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJSMI010000196 DAJSMI010000000
VERSION     DAJSMI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.200"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..116
                     /locus_tag="ON430_002562"
     CDS             <1..116
                     /locus_tag="ON430_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR3220445.1"
                     /translation="RVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            292..1035
                     /locus_tag="ON430_002563"
     CDS             292..1035
                     /locus_tag="ON430_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3220446.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1028..2608
                     /locus_tag="ON430_002564"
     CDS             1028..2608
                     /locus_tag="ON430_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCR3220447.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(2859..3338)
                     /locus_tag="ON430_002565"
     CDS             complement(2859..3338)
                     /locus_tag="ON430_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR3220448.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3354..>3620)
                     /locus_tag="ON430_002566"
     CDS             complement(3354..>3620)
                     /locus_tag="ON430_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR3220449.1"
                     /translation="RIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPG
                     VQITNAIRDLLAGHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
BASE COUNT         1143 a          622 c          687 g         1168 t
ORIGIN      
        1 gcagagtaaa cgaaatagta acatttgcaa atggagaaca actagagatg ggaaagagaa
       61 gtattgataa actaaagaat tttatgcaag aaaagataga aaatgaatca ttttaaagaa
      121 aacaaagact catatcttac ctgttagaag agtgttttcc tgtagaagag aacaacccaa
      181 gtagcagcct agtcgtttct atcctttttt gttatgcact ctttttgaca tttactccac
      241 caataacgaa gggtacaata aaaagtaata acaaacaaaa ggagtacaga aatgattgaa
      301 ttcatcaatg taagtaaaag ttacgggaaa aaacaagcat taaaagactt gtctctctcg
      361 ataagacaag gggaaatttt tggattttta ggacataatg gtgccggaaa atcaactacg
      421 attaaaagtt tagttagtat tattgaacca tcatcgggaa cgattttagt agatggaatg
      481 aaattgacgg aaaatcgatt gagtatcaaa caaaaaatcg gttatgtgcc tgattcccca
      541 gatatttttt tacaattgac ggctggagaa tattgggact tgatttcagc tgcctatgaa
      601 ttagacacac aaaaaaaaga aaagcgttta gcagaattga cagcgctttt tgatatgtat
      661 agtcatcaaa atgaaacaat cgcaagtttt tcacatggaa tgcgtcaaaa aacgattttg
      721 atcggcacgt tgctgccaga tccagatatt tgggtattgg atgaaccttt gcaaggactt
      781 gatcctcaag cagcatttga tttgaaagaa atgatgaagg ctcatgccgc aaaaggaaaa
      841 acagtcattt tttctacaca tgtattggat accgcacaac agttatgcga taaactggct
      901 attttgaaga aaggtgaatt gatttatcag ggggcagtta gtgaattatt gaatggctca
      961 cctgatgaaa cgctagaaaa gatttatttg aaaatggctg gaaggcaagc gacaggagat
     1021 ggaccgtatg tataagcgac aattattaga attaattcga gttaatcttt tatatgttaa
     1081 tccacagctc acgaataaag caagagagaa aggaaaaaag ggaaagcgat taactatcta
     1141 tcttttgtct caaaatcttt tttcagggct gattttttta ctgatttacg gatttgctat
     1201 gtttgctatg aactttgtta aattgccagg tttctttact tattacgtgg cactttttgg
     1261 tatgttaggt ttaactcaaa gtatatcagt catttataat atcttttttg aaggaaatga
     1321 cctgcaagta tttttgcctc tgccttttgg acaagaacag gttttttcag caaagatttt
     1381 ggtagttgct ttgaccatca ttccttttgt ttttccgctt ctagtcatgt ttattttaac
     1441 cggatggcga gctgatctag ggattttgct tactatcatt atctcaatcc ttttgttttc
     1501 tctatttttg attattcttt tttgtctggc tagtttgatt gtttttggtt tggctcgaac
     1561 agcagtgttt aaaaaacata gaaaaacagt ttctagtatc ttgcttggca gctcgatgct
     1621 gatcgcattt gctggaatca tgtggatgaa tcatcaaacg gggactattg aaaatgagtt
     1681 aacagatcgg catccgattt cgatattatt gccattctat gatgctgcca gtcagccact
     1741 ttctcaacaa ggtttattgg gttggggaat actagctatc acggctctgg gatttttcct
     1801 tgctgtaaaa cagctgatag taccaaagct tacagagcaa ctgactgatt caaacagtga
     1861 gttcaaaata aaaagaaccc ataaaaagaa ccaaaatgtg catcagcttt tatttagtta
     1921 caatgctcaa ttagtaaaag aacctaattt gattatgcaa gttctttctt cttccttgtt
     1981 aactccaatc atttttattc ttgcttttgc cctaggaggc gaaataaaat taactgattt
     2041 agataataag ttaatcggtg ttgttttcct ggtagggatc gcattggctt tcttaacagt
     2101 taatcagact tcttttatca gcaatcttat ttctttggat caagaaaatt ttctgtttat
     2161 ccgttctttg ccaatctcta tgaaccaata tttaaaagaa aagttcagat tcggatggat
     2221 attgcagagt tttttaactg gtgggatcgc attattaagc ggactgtctt ttcagctgcc
     2281 tttgtttttt atccctagct tgctatttgg ttctttattc ggctgctact tattgtcact
     2341 acgttatttc gctagagatt atcggttgct gctgctgaac tggaccaata ttaaccaatt
     2401 atttaacaga ggttcaggaa gtttgggttt agtcgcaact atgatgggtt cattgatcat
     2461 cagtattatt cttatcgtct tatacggaat gttggctttg ttttatccat tttggttagt
     2521 gaatattcct gtgatcggtt tagttctaat actttccgct ctatggatcg tttattatca
     2581 acgaacgttt tggaaaaaat ttgaataaag aagaggggcc gtgacaaaaa tcatgtcgca
     2641 gccccttttt tacagataga gggtgttcaa aagtcagttt ctcagtatta agtcaatttt
     2701 atgtaaaaat gtgagaagct atttttacaa aacttttttt aatactcata attacttctt
     2761 attgctcctg cggttacttg tcgcaatgtt tgtgagatat tttgtcatcg ctcaaggctg
     2821 aacgtctctt tcacaacctt ttctttgtta cacttcgttt atttaaaaag ccgttttttt
     2881 cgtttcatca tgattgcgtc acgctttact ttgaaattac ggatgttatg gttaaagact
     2941 tctgataata caagacctaa agcgattgct ccagcaatca taattgcttg gaccgcataa
     3001 tgagccgctt ctcgatagga ttcagtaacg agatttcgaa ccgcttgata agctaaacca
     3061 ccagggacta gaggaaccat cccgggaata ttgaaaatcg ttacgggcat tttcaaacgt
     3121 ttagagaaaa gatcgctgat gaatgctact cctaaagaac caagcaatga accaaaagca
     3181 gcagatccgc ctatttgaac acagatccag tagatcatcc agccaaaagc accagaaaga
     3241 ccacaacaaa tcaatgaacg cctaggtaca tttgtaataa cggcaaatgc cgcagtcgct
     3301 aaaaaactaa aagaaaactg aataaatatc gtccccatac ttgtgttcct ttcctaataa
     3361 aaaagctgga aaataaaagc aatcgcaaaa ccgatcatcg tagcagtcat catcgcttct
     3421 gttccgcgag aaactcctga gacataatgt ccagctagca aatcacggat agcgttcgta
     3481 atttgaactc caggaactaa aggcatcaca catccgataa tgattagatc ctgattgctt
     3541 cctaagccaa aacgtacgct gtagatagcg gctatgccaa tcaataaaga agataaaaat
     3601 tccgataaaa atttgattct
//
LOCUS       DAJSMI010000197         3609 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJSMI010000197 DAJSMI010000000
VERSION     DAJSMI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.201"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..680
                     /locus_tag="ON430_002567"
     CDS             <1..680
                     /locus_tag="ON430_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3220450.1"
                     /translation="NVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWV
                     IISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIY
                     FASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFY
                     NIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRF
                     DSDSALF"
     gene            692..1612
                     /locus_tag="ON430_002568"
     CDS             692..1612
                     /locus_tag="ON430_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3220451.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1653..3110
                     /locus_tag="ON430_002569"
     CDS             1653..3110
                     /locus_tag="ON430_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3220452.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3455..>3609
                     /locus_tag="ON430_002570"
     CDS             3455..>3609
                     /locus_tag="ON430_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3220453.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYG"
BASE COUNT         1151 a          626 c          736 g         1096 t
ORIGIN      
        1 ataatgtagc atttatcctt ttgaatctgc tgatctcagt cgtatttgca atcattatga
       61 gcgagttgag aaataaacga ctcgttaaag tttatcaaac gatgtcctta ctaccttatt
      121 ttctatcttg ggtcattatc agttactttg tttacgcttt tttaagtcct gacaaaggaa
      181 tattcaatca gtggatcact ggacatggag gagaagctgt caattggtat aatgaaccga
      241 aatattggcc gtttatcttg gtgttcattg gtacatggaa agggattggt tataacagca
      301 ttatttactt tgcatctgtt atgggaatcg atccgactta ttatgaagct gctatggttg
      361 atggagctag caaatggcag cagatcaaac atgtgacgat accacaactt attccactaa
      421 tgacgatttt aacgatttta gccgtaggaa acattttccg agctgatttt ggcctgtttt
      481 ataacattcc taggaattcc ggtgcattat atgatgtgac tcaagttttg gatacgtata
      541 tctacaatgg cctgacatcc acaggagatt ttggtatgac ggctgctgca ggactttacc
      601 aatcagttgt cggctttgtc ttgttgatga tcacaaatac gatcgcccga cgttttgata
      661 gcgattctgc attattctaa ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca
      721 agtccgctct ttcaataaga cggctaattt tttctctaat ataatgattg cactctttgc
      781 tatttcctgt attttgccat ttatatttgt tatcgccatc tcttttaccg atgagtccat
      841 attgtcgact cacgggtaca gtttttggcc agaaaagttc agtatatttg gttatacgta
      901 cttgttcgac cagatgcagg ataaaatttt ccaagcgctt ttcgtcactg tacttgttac
      961 tgttttaggt acactgatca atagcacggc tacttcttta tatgcttatg cgatttcacg
     1021 gtcaaacttt ccattccgtc gtttttttac cgttttttgt ttgattacga tgttgttctc
     1081 accagggatg gtggccaatt atcttgttat gacaaatctt cttcagttaa aagatacgat
     1141 ttgggcattg atcttgccaa tggctgtcag tcccttcaat attattgtga tgcgcacatt
     1201 ctttaaacgt tcggtctctg attcgattat tgaatcggca cggatcgatg gagctagcga
     1261 actgcgaata tttgttcaga tcgttttacc gttagctgtt ccaggtattg cgacaatcag
     1321 tttatttgcc gcattgggtt actggaatga ctggttcaat gctttactat acatccaaga
     1381 tgataagcta gttccactac agtatctttt gatgaaaatc caaagtaata ttcaatatct
     1441 gacacaaaat tcaggagcag gaagccaatt gacaggcgga ttagcctcag ttccaggaga
     1501 atctgccaga atggcgatcg tagtcatctc gacgttgcct attgcgatca gctatccatt
     1561 tttccagaag cattttgtca aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa
     1621 aaatataaat cataaaatta ggaggaagaa aaatgaagtt atggaagaaa ctagccttta
     1681 caggtgtttc agcgttgttg ttgggaacat tagcagcatg cggaggttct ggttctaaag
     1741 accaggcaga agctagcaat tcggacactt tgcagatgta tcaaatcgga gataaaccag
     1801 ataatttcga tcaattgatg gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa
     1861 aagtcaatat caattacatt ggctggggcg attacgaaaa gaaaatgaac gtcatcattt
     1921 cttccgggga aaattatgat atcgcctttg cgaacaacta tgtttccaat gcccaaaaag
     1981 gagcctttgc tgatttgact gaattagcgc ctaagtacgc tgaaaaagct tataatgatt
     2041 tagatgaagc atatattaaa ggaaatctgg tcaatggaaa attatatgct ttcccagtca
     2101 atggaaatgt ctttgctcaa caagtactta cttttaacaa agctttacta gataaatacg
     2161 atctttcaat tgatggaatc caatcttacg ctgatgcaga aaaagtcttg aaggaattcc
     2221 atgaaaaaga accaaacacg gcagcttttg ccattggaca aggcttcaaa gtccaaggga
     2281 actttgatta tcctttaggg aatacgttac catttgctat tgatctggat ggagatgatt
     2341 cgaaaatcat caaccaatac gataatgaaa cgtatatcgg actgttacgt acaatgcata
     2401 gctggtacaa acaaggactc attccaagtg acgctgcaac gagcaataag gattatccac
     2461 tagaaggaaa tacttggcta atgcgtggag aaacacaagg accgtatgat tatggcgata
     2521 caattttgac aaatgcagca aaacaagaac ttgtctcaaa agcaattact gttccactaa
     2581 aatctacagc tcaagcacaa atggcaaatt ttgtcgtatc aaatacttca aaaaataaag
     2641 aaaaatcagt agaacttcta gggctgctta acagtgatcc agaattattg aatgggcttg
     2701 tttgggggat cgagggagaa gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat
     2761 tgctagatgg ttatcagcca aatacacata tgtctgcttg gaatacagga aacaacaaga
     2821 ttctatatac acaagagtct attacggatg acatgattgc caaacgtgat caatcgattg
     2881 ctgatgcaga aacttcaccg attttaggat tcagcttcaa cactgacagt gtcaaaacag
     2941 aattaagtaa tatttctaat gtcatgaatc aatatttaga tggattgaat acagggactg
     3001 ttgatccaga tgaaacatta ccgaaattga aagacgcttt ggacaaggct ggctatgata
     3061 aggtattgaa agaaatgcaa aaacaatatg atgagttccg acaagaataa taatgaataa
     3121 agacaaaggg gttgtgacaa aagacgtgtc acaacctctt gttccgaata aacggtgttc
     3181 aaaagccagt ttctcggtat taagctaaat atatgtaaaa atgtggaaag ccatattcac
     3241 aaaatttttc ttactactcg aaactaagcg gctatttcac aaactcttgg taaacggtgt
     3301 tcaatcagct aaccctcggt attcgctcac aaaacgacaa aatatgagga actatttttc
     3361 cgtttgtttc tctaataatc aggtcaaggt gctgattgcc caaccttttt ttgtataatg
     3421 agaaagtata aacttaatgt ttggaggaga agatatgtta gggaaggttt tggaagagtt
     3481 atttatccgg atctgggtca ttataaaact aaccttgtat ttctggatat atacatttgc
     3541 cggcgggatc atctttggat taggtgcggc atggaaaaca gtcaatgaac tattttattt
     3601 gtacggatt
//
LOCUS       DAJSMI010000198         3554 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJSMI010000198 DAJSMI010000000
VERSION     DAJSMI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.202"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(561..1601)
                     /locus_tag="ON430_002571"
     CDS             complement(561..1601)
                     /locus_tag="ON430_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3220454.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1950..2276)
                     /locus_tag="ON430_002572"
     CDS             complement(1950..2276)
                     /locus_tag="ON430_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220455.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2263..3066)
                     /locus_tag="ON430_002573"
     CDS             complement(2263..3066)
                     /locus_tag="ON430_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3220456.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1089 a          585 c          490 g         1390 t
ORIGIN      
        1 tccgttgcgg gtaataccgt aacactgaag ggcacgcatt aaggcgtcat cttaatgctt
       61 tcctgtagga cactgacgga gattaccagt ctcccgagcc ttacaggggc ctttcttttt
      121 tttgtatttt caattatcac aatttatcgt ccaaaagtgc agaacgaaaa tcaattcatc
      181 acaagcatat cagacaatta aatttaaaac aactaaaaat atacaaaaaa gtgtaattga
      241 atattagcag cactgcctga aaagaagtaa tatcaggctt ttcagcttgg ggaaataagt
      301 gaattacctc tcttcatgaa ttattataac tcaaaaaagc acatatgcaa acactagttc
      361 cctatttttt tgaatgaaaa ataactaaaa tatctaacga ttatgacttt aaaacctacg
      421 tttgtgattg ttttcataaa cttttaatct aaaaactccg gaataatagc tatttatttg
      481 taacatttgt aataaaatta aaatcataaa aaagttctaa tttaaaattg atcattttta
      541 tagtgatcgc tttttatcaa ctactctagc caattttccg tactaactgt tggtaattcg
      601 ttaaatgttt ctctttgttc gtttgcaagc tttgccgcaa tagctttttc gacaaaccaa
      661 ttttttacat aaacgaaaat tagattctgc aaattgttta ctttttcagt tttttgtttt
      721 tgataagctt tcagcaaagt catatataat cctttgtcag catcaattcc ataagattcc
      781 agttcttcat aaacaacaac tgtttgctct aattcttcag cttttttctt tgcattatga
      841 atagtttgga cggttttttg agcgtcttcg taagtactag aaaatgtttt tatcaagttt
      901 aatacatttt ctggtacaaa tgtatttata ctgtcctctt tcatgatgtc gacgaagtta
      961 tctagtaaaa cttgattttg taactgatca gtttcagtgt ctattatgtg tctattagtg
     1021 tctatagaat tagaatagaa tagattgtct gcgattttcg caattacggt tgagtccttg
     1081 atatgactgg attttgccgt atttgcgatt ttcgcaatta cggtggtatc attgatttct
     1141 cgcggttttg ccgtatttgc gattttcgca attacggtgg tatcagtatt gtcttcctct
     1201 tccatgattt taaagacgtc ttgattcgtt acttctggct tgcctaagta caataaatta
     1261 ggcatggatt caatcttacc gtcacgttta tttactcttc ctctcttctg tttcagaaga
     1321 cctgcttttt ctaattcctt tttgatttta gatactttac cttcgcggca ttgtaacaat
     1381 ttcattaatt cttcaactgt gaatatgaaa aatatattat catcagaatc tacccagtta
     1441 tttttgactg aataatcaaa tcgatcttta agtagcatat aagcaatttt tgcatcattt
     1501 gatagatcca tgtatttttc gttagtaaaa aataccttag gcatttgata atatttttca
     1561 cgataaattt cttttgattt gtaataatta aaatcattca tagccaatcc tctttctgta
     1621 ggatagcgtt ttgttcaaaa ctatgttaca atgaattaaa caaatatatc tatccactat
     1681 atatttttct actgctgata agtcgccaaa ctttgttaaa gcagtagtct ttttttatct
     1741 ttttttagtt taccttaaaa aataaattaa agcaaaccgc tctatttcaa ggtttaagct
     1801 catagtaact atatcacgat attacaataa tattatatca cgatatcgta atattacaat
     1861 atgaagataa gcgacttatc agctttgata ctactgattt ttataaaaaa aatatcacga
     1921 tattatgata tcgtgatatt gaatgtaaat taaattttat tttgtttctt tgctagttta
     1981 tatccttcaa tcaagtattc ttcaggtatg gactttaata gcgcatcttc tataacgtct
     2041 ggtttacttt tgtctgattc agcaatagtc gtccaatatt tgagtgctga cattagaatt
     2101 ggatttatac gaatgctatc tgctgttctt acactacttc cttgatcttt tatgacttta
     2161 cttatatttt tcttggttaa ttcgatttct tctacttctt ttgatttaaa attcttttta
     2221 tttttatttc ctagtaatct acttactgca gcttgactgc gattatcttc tgccatcaaa
     2281 atcaccaact tcttttaaat attcttctgt aaatgcttta tagtcatctt cagctgttga
     2341 gggtttatta aaattgatta gatctgatcc agcagcttgc gcttcgtcaa cggttattgt
     2401 cctacgaata taagttttga aaatttttgc accaatgact ctagaaattt cggttgctgc
     2461 agacgtcatt tctttagaaa tgttataacg cttattatac ttagtaaata gaactcctcg
     2521 tacttcaagc ttgtcgttcg tatattcttt taccgtttca atagtttcac ttaactttat
     2581 tagtcctttt aatgagaaat acgcagcttc aactggaatg ataatttcat cagaaacagt
     2641 aaatgcgttg actgtaagca ttcctaaact tggtggacaa tcaattataa tataatcata
     2701 gttgtccttg attggttgaa gtactctttt aagcctgtgt tcacttccta cttgagtaaa
     2761 ttccctctct gcagaagaaa gggctatgtc ggctggcaaa acatcaattc ctttatagtc
     2821 cagaatagca tctgtaatgt cagcctcccc ttttaatact tcataaattg taggttggtt
     2881 tgttccatct atatcgaaac ctacattatc tgtagcattt ccctgaggat caatgtccac
     2941 taaaagtaca tttttaccgt aaacaaattt caaacatgct gctaaattaa cgctagtcgt
     3001 agttttagaa actccacctt tttgattggc tactgacaat attcttgcca ttaaaatatc
     3061 ccccattcat cttatattat aatataatta tatcataata ttgtgatatt gcaatatcgt
     3121 atatgcatta tattgtaata ttgtgtgatt attttgtttt aaagatatat atcggttcgg
     3181 atataaagat tattcatatg ttattcgaaa ttaaaataat cactttttat tactttaaaa
     3241 ttttttatta atgataagaa atatgttttg cgagcagcaa ctagaataat ttaaacatta
     3301 ctaaactgtc tatattgtta ctaataattc ccttcttata gtgcattttc ttagaaaaaa
     3361 acaatattac gatatcatga tattaagata tcgtaatatt atacaattgt gattgatatt
     3421 atatatgttt ttttattttt ttatcatgtt taatcttgaa ggaatcatat tctttttcta
     3481 aagacaggag cttttcataa acatcagaca tgtttaaact agaggccagg cttaaatcat
     3541 aaacgaatga agca
//
LOCUS       DAJSMI010000199         3480 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJSMI010000199 DAJSMI010000000
VERSION     DAJSMI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.203"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..2232)
                     /locus_tag="ON430_002574"
     CDS             complement(<1..2232)
                     /locus_tag="ON430_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220457.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ON430_002575"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ON430_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          977 a          769 c          549 g         1185 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
//
LOCUS       DAJSMI010000200         3450 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJSMI010000200 DAJSMI010000000
VERSION     DAJSMI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.204"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            317..1102
                     /locus_tag="ON430_002576"
     CDS             317..1102
                     /locus_tag="ON430_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3220458.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1115..1966
                     /locus_tag="ON430_002577"
     CDS             1115..1966
                     /locus_tag="ON430_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR3220459.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2090..2542)
                     /locus_tag="ON430_002578"
     CDS             complement(2090..2542)
                     /locus_tag="ON430_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3220460.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
     gene            complement(3007..3375)
                     /locus_tag="ON430_002579"
     CDS             complement(3007..3375)
                     /locus_tag="ON430_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCR3220461.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         1102 a          564 c          680 g         1104 t
ORIGIN      
        1 ggctgtgaca aaagtcttgt cacagccttt ttttccaaat aaatggtttt tcagaagtag
       61 cttcttcgga aataagccga aatttcacaa aaatttgaga agtaattttt gtaaattact
      121 tcttatttct tgaagctggc cacttctgtc acaacctctt tctttataga aataaaagga
      181 cactagcaat tcatgaaaac gattgctaga gcgtatcatt tgctttagac tatatatctt
      241 ggtgtacact cactttaaga cggaaataaa agttatccgt aataacaggt ttaggtatga
      301 aaaaggagtg ggtactatgt ataaggaatt agaaaacaaa gtcgcagtag taactggtgg
      361 ctcaaagggg attggaacag cgattgctca acgattcggg aaagaaaaaa tgaatgtagt
      421 tgtcaactat aactcggata aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc
      481 aggcggaaaa gcagtctctg tacaagcaga tgtcagttct gaagaaggaa tacagcgttt
      541 acttgatacg gctttagaaa attttgggac actggacgtt tggattaata atgctgggat
      601 ggaaaatcag catccaaccc atgaacttag ccttgaagat tgggaaagag tattaaaagt
      661 caatttgact ggcgttttta tcggaacgaa agcggcttta aattacttta cagagaatga
      721 taaaaaagga aatatcatca atatgtcttc tgttcatgaa caaatcccat ggccaacgtt
      781 tgctcattac gctgcttcaa aaggcggagt aaaactattc accgaaacgg tagcaatgga
      841 gtacgcacca aagggaatca gaatcaatag tataggacct ggtgctatcc gtacacctat
      901 taatgcggca aaatttgatg atcctgaaca aaaagaactc ttggaaagta tgatcccact
      961 aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg cttcagacga
     1021 atccagttat gttacaggta ttacactatt tgcagatggt gggatgactt tatatccttc
     1081 tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt ttattgattg cattgatccc
     1141 ggcagttggg tgggggatcc agccactgat tttgaaaaag attggtggca gaccaacaaa
     1201 tgagatatta ggaactggta ttggagccgt gctcgttggc ttacttgttc aattattcgc
     1261 ttctccagct ggtatttcag tgactacatt tttgattagt ttactttctg gtgcattctg
     1321 ggtcatcgga caaacaggac agtatacatc tttcaacttg attggtgttt ctaaaacgat
     1381 gccaatttcg actgcattac agttagtcgg aacctcattg attggtgtct tcgcttttgg
     1441 agaatggtct ggaagtatgg gaaaaatcat tggtgcagca gcaatcattc ttcttgtatt
     1501 tggttctgct ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg
     1561 aatcagtcta ttagcatcaa caagtattgg ttattgggtt tacagtgcat tgcccaaact
     1621 agttgatgca agcggacttt ccattttctt tccgcaaatg atcggtgtct ttttaggggc
     1681 agtgatttat gtgattttcc gaaaaccaac cgcattttct gaagacaaga gttggaaagc
     1741 aacaatagtt ggagccatct tcagtgtgtc agcattggct tatatttttt cagcaaatga
     1801 aaatggggtt gctactgcat atatcattac acagctaaac gtagttatct ctacattagg
     1861 cggtatgtac atcctgcatg aaaataagac accaaaagaa aaaaaattaa cgattatcgg
     1921 attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat aaagatggta
     1981 aaatctcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt ataaaaaaat
     2041 agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt taagatacaa
     2101 gtgaaatatc tgtctgcatc aataattttt cattgatcca ttgactaaag tttacgacat
     2161 aattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga tcgtattgta
     2221 aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat tgttctcgga
     2281 attgattgat tgctgtaaaa tcacgatact gaaggttgaa acgcatcgtg attggtgcat
     2341 ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg tataggaact
     2401 caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct tttagttttc
     2461 cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg gctaattcat
     2521 ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta ctgacttagt
     2581 tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa
     2641 tcaagtcaga aaattctaac ttttctttct ctaaagaaat tcgttctctc ctaaagtgat
     2701 attatattta aatatacctg aaaataatgg gcagtattat gaaagatcac tgatattttc
     2761 tcgtattttt tccttattca aaagttttgc aggaaaattg ctgaaaaaat aattttacta
     2821 agtggaaaat tcaaagccat tcaatcagtg atttttttca gcacaagaaa gactccttat
     2881 aaaaatagca gatactttgt caattaagtg tttaaccagg gatgataaaa aggataggat
     2941 tgaacaaaac ctgtataagc tgatgaatag tttcctcgcc agtaaaaggt ttattcgtat
     3001 aaagctttat cgccaaaatt tatcatgacc ttttttatct tcaatattaa atgaaatcaa
     3061 ttgttcaatc aattcccagt tgattacact tttccaagga actttgatga cgttatctgt
     3121 atatacgtag cctgcttctt ggatattttt ttcaagggca cgaatcgctg ctttttccgg
     3181 tgcgatagaa aaatgatttt tagcaaaact aaatgcaata ataaaggtac catggtcagt
     3241 aaacataggt tggttccatt ttacagttgt gtctagctgg ggatacgttt ctgttatctt
     3301 agcaaaaagg tctttgatct tttgttgatg ttctttttct ttgatctgat tgatgatcgg
     3361 ctgaaatttt tccattctaa aacgcctcca ttaagtggtt tgatttattt taccagaaaa
     3421 acaatagtta tgtataaaaa acgaacaaaa
//
LOCUS       DAJSMI010000201         3315 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJSMI010000201 DAJSMI010000000
VERSION     DAJSMI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.205"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(76..306)
                     /locus_tag="ON430_002580"
     CDS             complement(76..306)
                     /locus_tag="ON430_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220462.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(251..409)
                     /locus_tag="ON430_002581"
     CDS             complement(251..409)
                     /locus_tag="ON430_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220463.1"
                     /translation="MDVDTSEILYDIEIDENLLDVAQEIDHQKQEAIRYAGRKGKRKY
                     LLRECSKS"
     gene            complement(400..738)
                     /locus_tag="ON430_002582"
     CDS             complement(400..738)
                     /locus_tag="ON430_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220464.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1666..1782)
                     /locus_tag="ON430_002583"
     CDS             complement(1666..1782)
                     /locus_tag="ON430_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR3220465.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(2258..3136)
                     /locus_tag="ON430_002584"
     CDS             complement(2258..3136)
                     /locus_tag="ON430_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCR3220466.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         1059 a          604 c          483 g         1169 t
ORIGIN      
        1 agtaatatta acagcaaaga tatcgaaaaa acaataacat aatatgtgtc aaaatttttc
       61 ataataagaa ctcccttact gttgcatact aacataatcc aaaaactctt ctacttcagc
      121 taaagacatt ccactttctt tagtccaaaa aacagttttt tctatcgatc cttctctgta
      181 aactgtaaat gtcggagtcg agttaattga gtagcgactc ttgaattctt tccactcatt
      241 ttcatcttct ctaacttttg ctacattcac gtaataaata ttttcttttc ccttttctac
      301 cagcatatcg aatagcttcc tgcttttgat gatcgatttc ttgtgctaca tctagtaaat
      361 tttcatcaat ttctatatca taaagtattt cactagtgtc tacatccaca atatatactt
      421 tttatcagta gcaatttttg agtaatccgt taaaatgatc tcagtctcgt cactttcatc
      481 agtataaata tacgttaaca atgaactcac cttttcaatc ggtgttagct ctaagcctac
      541 tgtatttttt actattgagt cattatttac caccataact tcgtactgta tataccatcc
      601 tatttggttt ccatcaatat cagtaattgg ctcactagct aatggagtct ccggaacaat
      661 attttcctga tatttcttaa actcttctgt agcacgaaaa aggttatcat gttcctcact
      721 tgaaactggt tgagtcatag aaaatgttac ttggctaaac agagggaaaa taaacactac
      781 aatcaacaaa gtataaaaaa aagcccttct ttttcatttt ttatcttcca atcacaaatt
      841 gaaaaactta ttcctgcaac tttgagtcta gtatcacaat cgactgacta caactattca
      901 tttacattct actttaagtt cctccttaac cattaatttt attaaagata cttgtaatag
      961 tgattctcac aaaatttcat gaaaaatact ttattactgt atctaaaatt ccaatacaca
     1021 acttatcgac tattatctga aaaccaaaac ttattattcc ttgatgttat tctagcaata
     1081 gtaactgctt ttttattttt ttgcatattt tttcggcgtt actttttaaa gttaactttt
     1141 tcgacaaata gaaagagata cgaatcactg atttttttga agaaaagaaa tggagtgagc
     1201 tgaacgttag agatattggg agctttcgta actgcttatt aaacgataat ggttatttgc
     1261 aaccgtattg ctcgcttcta catgaaatat ttcatgaaat atttcgcaaa acctttgatt
     1321 atgtagttac gtttccattc ttgactaaaa atgtttcaaa aacgaacaaa ggtgttaaaa
     1381 ggtaaagtga tcaatactac tggtcaaaat aagaggttga aagggaattg aaaaataact
     1441 acagataata cttttataaa cgtacgtgct ttgtggttct gtagttaact atataactgg
     1501 aattagggaa tggtatcaat ttgaaaagaa agattctacc aatttatcat atgttaaaaa
     1561 tggaaaatca acagcagttc tttcggaaac cttacacaaa aaccgattat tgaaaatagc
     1621 cgaaaaacgt ttttcggcta tttttttaat taagtttcct tcttcttacc ttttcccact
     1681 attccgtttc acatctttga tgatcttcag cgcacgtttt cgtgttttga tatttgggct
     1741 tttcaagcgg cggtatgcac ttgagatatc tgttttttcc atatggacga cccactttct
     1801 tctttgattg tagtcatgct aaaaaacaca aatcgaaaca attacgattt gtgttttaaa
     1861 tggtatcaaa agttaaaatg aaagtcaact tgttaaaaca gtcgatcaga ctagctgatt
     1921 gactcctatc tctggaaata aattttcatt attacgtttt agctacctaa aaaaggccct
     1981 cttgtccgtt aactttaata tctattgtta tagctagttt acctattgaa cgtactgtgt
     2041 gttcgataga acactaaaaa agaggctggg acagaagcgt ttaactccaa gaaataagaa
     2101 gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt
     2161 gcttctgctt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt
     2221 gtctcacgct cttttaaact atttcgaagt actaaactta tgaagtaaga atacctaagc
     2281 cagagccaat gtgctcgcct gcaagcaatt gctcttttgc ttgtcgctgt aaactcgtac
     2341 tttctggata atcatgtatt tgggcatcta ctcgtccaaa gatccaaggg actccactat
     2401 cgtcctgttc atcaactgat aataatcgat aatcggctgc aaaaagacag atttttgact
     2461 cttttttcaa ccatttagta agattgggtg atagtaacca agtatctgtg tacatcactt
     2521 ggtaatggta atcaggaaaa taaagttcaa aaaaacgaat ggcactttcg taactatctc
     2581 ttatcttatt ctgagataaa tctgcatcgg aaggaatatg aagagaaagg cactgctttc
     2641 cttctaatct tagatccgtg taccctttcg gaaaatatgt catttcgtac tccaattcac
     2701 caatccgaaa gatcaagcca ctagtgtaat gccaaatcca aaaaccacgg tcaaagcgat
     2761 agcttcctgt tacttggaaa tgttcatgaa taaatctagt aaaagcattc attgttgcca
     2821 aatagatagt tcttggaatc ttcaatggtt gataaatttt tttccaactt tgagaagctg
     2881 catatagata aatagctaat gctgcaaaag gttcctcttc atagactttg tctattttct
     2941 ttgcagtcgc ctttgcttta ttaggatcca ttagttcatg actatattgc tcaaacacct
     3001 catcaaggat gttttttgct tgtacaatca tttgctctat aacgactttg ggaagagtta
     3061 aagtttctgc cagaatttgg atttcattgc ttattttctt catcaaattt ccccttttca
     3121 tatctttgaa cctcattgtt taaatgactg attatccgga caccatatgg tataaacata
     3181 cttttattgt acactaacta taaaacttaa actttctatg aagaaagaat atctgtaaga
     3241 ctttcaagga aagcattgac catctcagga aagtttattc agttaccatc ataaacaaaa
     3301 ttttttgata gaata
//
LOCUS       DAJSMI010000202         3293 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJSMI010000202 DAJSMI010000000
VERSION     DAJSMI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.206"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            71..379
                     /locus_tag="ON430_002585"
     CDS             71..379
                     /locus_tag="ON430_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCR3220467.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQLPLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY
                     "
     gene            406..705
                     /locus_tag="ON430_002586"
     CDS             406..705
                     /locus_tag="ON430_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HCR3220468.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEIGTLPKDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            879..1430
                     /locus_tag="ON430_002587"
     CDS             879..1430
                     /locus_tag="ON430_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCR3220469.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1576..1869
                     /locus_tag="ON430_002588"
     CDS             1576..1869
                     /locus_tag="ON430_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220470.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1862..2158
                     /locus_tag="ON430_002589"
     CDS             1862..2158
                     /locus_tag="ON430_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR3220471.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2233..3231
                     /locus_tag="ON430_002590"
     CDS             2233..3231
                     /locus_tag="ON430_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCR3220472.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1237 a          456 c          654 g          946 t
ORIGIN      
        1 ttatatccat gaagcccaat cttctttaat agaagattgg gctattttta tagcaaagga
       61 gggaaaagaa atgtttcaaa ataaaggaca acgttatgtc acaaagggag taatggacag
      121 tttgccggtg gaattacaag ctttatgttg gaatctaatc gatcaaaacg ttcaaaagca
      181 acttccttta gactatcttc aaatttttga attttctacg gaaaaaggaa atcaaaagtt
      241 ggttcatcgt caagaagaac cggaagaaag aaaagagtat ctgatttcac ctaagcttcg
      301 tttgaaatca gtgtctcaaa aaatatgggc cattgattcg ggtgagtatc aaacgatgtt
      361 attaccagaa gaatactagt atctaaaaga aaggaacgaa aggacttgtt agaaacggct
      421 atcgaagtac tggaaaagtg tgcacagctg gtcactgctt ctgaagagtg gggctatgaa
      481 tccgttacga tggaaaaaga ggaaattgaa ataggtacac ttcctaaaga tgttcatcta
      541 ccacgtttag tgatgacaca tctttatatt tattgcgcac cagaagatgg aaaagactat
      601 gtagtttatt tcattacaga tattaccagc cagcgagaat ttgttcgtgg gttactggta
      661 gaaggacgat tggtttggag tcagattgga ggaacggatg aataagatcg attatttgag
      721 aatgattgcg aaatgtttag aagatatcct taccgcgatt catcaaattc tgaagaaaaa
      781 aggagaaaga atatgaaata ttttcaacta gtattgacac ttataacggc aatggtaatt
      841 gaagtgcttg aacaaaaaat agcataggag aaaaagagat gattgaaaaa gaattagtga
      901 ccacacaaac agaagtttgg cgagatcctc atcataccca tcgctatgtt ttcaaacgac
      961 aatgggcgat caaaggaaaa gaagcggaag ataaattagc agtagtcatt actattcgtc
     1021 caacagatac agaaccattt acgaatgatt tgagtatgtt attaatcgaa aagaatatcc
     1081 gacaattagg atttactggt tttattgctg tgaatctttt ttcgtacaca aaagcgaaaa
     1141 gtcctcgggt atttcttcga ggaaatgatg aacaatcttt ggaaatatta acgactgttt
     1201 ttacagaaaa gaaaatcaat accattattt ttgcttgtgg ttcgattaca gcgacgagtc
     1261 aagtggctta tgaacaagca aagactattt acgatcaatt gagtgcgaaa caaaagaaga
     1321 tgaccaaagt attgataaat gcagaaggta agttatccca cccactaagt attcatgttc
     1381 gtaatgaatg gcaattggca gatagtcaat tattatttca agaaaaataa cattttattt
     1441 tctcatgtta cttataagta gttttacatg ctttttgact ttaaaaatac atgtgatgat
     1501 acatgttata tgaattatca atgtacccag taatatgcta ttttcattat gtaaaaaata
     1561 acagggggtg tgtagatgga gattgatgaa acttgtttga gtaactctta taaaagtatg
     1621 aatgactttt ttgaggaaat ggaactggat tcagaattgc tctatcaatt ttatcttgct
     1681 tttcgagggc aacagatttc ttttccaatg aaaatgtacg atcgtgagtt agttagaaaa
     1741 agaatcgaaa acatgataga acaggaaaaa actgtagata tccgtcaatt gacagaagtt
     1801 tatggtttct ctgcaagatg gattcaaaaa gtaattaaac aaaaagaaag gagacgagta
     1861 catggctaag ctgattgtat taggtggttt aatttacggt ggatattggt tatataaata
     1921 tatgcttgcg catccgaata tagataaagg aaaagtaatt aagacagttg gggtaatcat
     1981 agttggactc atagctctaa ctaagaaaaa taatactaat agcccaaaaa taatgagaac
     2041 ttatcgctgt agaggatgtg gaagggaaat ttctactagt tttaatccat ctgagagtcc
     2101 tcaaggaaga gaccgctgcc atacgggcta tcgacattat tgggaaagaa tcgattaaaa
     2161 gattaccatt tagatcaatt aattgtctga tagaataagt acgtgaggga gaaattgaaa
     2221 gggggaaata taatgaaaaa atggctgtta ttactaatag gaatgggaat tttattcact
     2281 ggttgtacat ctgagaatac gaggactaaa gcgactatag aaactgatac aacaattgag
     2341 actaaaaaaa gtaaaacgca atcaactgaa caaactacaa cagaaatgaa gcaatctgtg
     2401 gaaacaagtg caagttcaat cactgaatct tcttctgaag aggttaagaa tactttgtgg
     2461 gatacgaata aagcttccaa attagaaact tttgtcacac aatggggaaa gacattaggt
     2521 caagaatata aaagctacaa tcttcagaat aatgtaagtc tgtatggcac tcctttacca
     2581 caagccgtga ttaatggcga ttggaaaatg gcaatcaatg aagcaccagt taccgtacaa
     2641 tggtcagaag atggaactgg tcatgcagat ttcaatttag tagcagttta ttctgatgta
     2701 gaaactggag aatatttagg gcagcatgtt tactttttcg gttttcaaaa tggacaacct
     2761 aaagtttatt taacgcaaca aaatcaaggt aatgaaaata attatctgta ctttaacgaa
     2821 actcaaaatc aacagttaaa acaaggtttc agtgatattg tgaatggaca aactcctcaa
     2881 acaccagtag tagaagaatc tactcaacaa gcaacaacta caacgacaca atctacaagt
     2941 agttcaggaa tttcaagttg ggaagctgct aaagaatctg tgctaaataa taaagcttta
     3001 tggttgtatg attctcctga taaaaatgta tcaaatacta atttaattag cacaaatgaa
     3061 tatatgaaag atctaatgca agatgaaaat ggaagctact attcaattaa tattgtaacg
     3121 gatgatgatg gaaacacgac catggctggt ggaaaacaat ttcgtgttta tacagatgga
     3181 agaatttctc aacgtattgg aatgactaac aattttcaaa tgattaaata attgaaaata
     3241 ttatttatca aagaaaaact gattttgaga aaaattattt aattttctta gaa
//
LOCUS       DAJSMI010000203         3242 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJSMI010000203 DAJSMI010000000
VERSION     DAJSMI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.207"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            62..640
                     /locus_tag="ON430_002591"
     CDS             62..640
                     /locus_tag="ON430_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3220473.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            769..1146
                     /locus_tag="ON430_002592"
     CDS             769..1146
                     /locus_tag="ON430_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCR3220474.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            1079..3151
                     /locus_tag="ON430_002593"
     CDS             1079..3151
                     /locus_tag="ON430_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3220475.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         1200 a          595 c          683 g          764 t
ORIGIN      
        1 tgaaagtctc ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt
       61 tatgagagga aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg
      121 ctttcgagaa ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa
      181 acgcttggat atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa
      241 aacagcgcta acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt
      301 gggaaaaagt ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt
      361 ttcaaatcaa gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt
      421 attagatgga atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt
      481 aacgaaagaa gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct
      541 ttccttacgt tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta
      601 tttttggatg atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa
      661 caattttaag atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg
      721 aaaagtgact aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta
      781 attaccatgt tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt
      841 gaagaagaag aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg
      901 aacgaaatcc atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt
      961 tggaatgacg gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag
     1021 ggacttgcgt tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat
     1081 gaaacaacta attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg
     1141 tgctaatcaa aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct
     1201 tggtcattta ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca
     1261 aatggacacg ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg
     1321 acatcaatta aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat
     1381 tgcgacagat gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca
     1441 tttcaataaa ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac
     1501 tggattcaag caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc
     1561 ccgggcaaaa gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta
     1621 cgcggataat ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca
     1681 agaaaagaaa atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg
     1741 aaaagaacaa gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc
     1801 acaaaaactt gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa
     1861 attaaaaacg gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa
     1921 tcaacgctat ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac
     1981 ccataagatc gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag
     2041 tacgatgaaa gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt
     2101 gaaaaacgga gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca
     2161 ccatgcactt ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg
     2221 aaaaatctat caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt
     2281 cagccaaaca aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg
     2341 tgtcgtccaa gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga
     2401 aaaaggaatg agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa
     2461 accattgtca gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc
     2521 tacgcgtgca gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca
     2581 aggactgcaa gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt
     2641 aacaccagat ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa
     2701 acaagcagga accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat
     2761 caaatcaagc aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg
     2821 tggggccaat ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc
     2881 agattgcaac taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg
     2941 tcaccggaaa atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga
     3001 tggaacgaca gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga
     3061 agaacgacgc ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc
     3121 agcagcacta aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt
     3181 ggtcagcttc aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat
     3241 tt
//
LOCUS       DAJSMI010000204         3205 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJSMI010000204 DAJSMI010000000
VERSION     DAJSMI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.208"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(1646..2164)
                     /locus_tag="ON430_002594"
     CDS             complement(1646..2164)
                     /locus_tag="ON430_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCR3220476.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2531..2779
                     /locus_tag="ON430_002595"
     CDS             2531..2779
                     /locus_tag="ON430_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220477.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1079 a          568 c          454 g         1104 t
ORIGIN      
        1 ataaagaaaa aattcaagca acaattcaaa aaaatcttaa ctgctgagat tgatgtagca
       61 atatctcaat catcaacaaa gtacgatcct gcctttcaaa agcttattga taaatgttta
      121 ttcattataa aaataaaaga aaaatttatg tcagcttttt tcatcaaagt ggtaatgata
      181 attcgtctat taattgtatt ttttaggaaa tatggtataa ttgttttaac gaaaaggtaa
      241 ttcatgatac accaaaaacc actagctggc gggctagtgg ttttctttta cgttctattc
      301 agattaacgg gtaggcttac tgtctttttt ggcaataatt ttgcaatcaa gccatttaca
      361 gatgtaatgc gcggatactt ctgctaagat tgcaaaaaac atatcgaaaa ggtaattcat
      421 aatacaacct cctccccttg ccataatagg ccggggtagc agacagcata aatattatac
      481 cataactgaa aatttcattt tagtacattt caatatattg atgaaccagc acaataaaac
      541 atgccatgac taacagaaaa aaacttatta aaccagatat aacttgtaaa catccaacta
      601 tatagaaaac gtaaattttc tatatagttg gattaagata acgactattt attttatcat
      661 atttttacta taccctatac ttttacttgg tatatttatg aataatcgca aatttacccc
      721 tatactacta gtttgtaagt tagattaata aaaatactaa agttatttgt tcttttatta
      781 aatacaccta caacttataa attgatggta tttagataac acgaaagaat aaaaataaca
      841 gctgatagta aagtttgttg acgattgcta ctcaatcgaa agtgagttag tgcgtatgaa
      901 cttgcttctg aaaacttatg aaatgaaaga ccttttacat cttgcagaaa tcgttactgg
      961 gattaaacta agcaataaaa actaatgttc ccctcattct ttgaacttct actatttgat
     1021 ttatttattc tgataaatga cgatttcacc ttcaggatct tcaaatcccg tatagaattt
     1081 atttaagatc tcttcagtat cagtaatagg ataggcgata ggccacatac cctttgatgg
     1141 ttagaataaa ctgctcaaaa tctgtatcgt atgttggctt tagcatatta acaaaggcta
     1201 tctccaaatt ataaaccata agcgttgaat cgttcttaac aatttcaatt cttcctaacc
     1261 caaagtcaga agatataatt accggacttg ccttaccctt catgtgggtt gttgagattc
     1321 catatttaaa gggaattgtc ggtactatcg gcggtctgaa aaacatccca aaaataaaaa
     1381 aatctttccc ctgctagtct cctaagtccc tttgtagcgg atatggcacc ataatctcat
     1441 tttatcgaag tgagtcatgg tttatctgtt ggccaaccaa aattgataca atctaacgat
     1501 gcaccctcta cggcattcta ggggagtgca cttgcattca cccaataaaa accggcagtt
     1561 agcaaagaac ttatgtagtc gtatgttctt actgttaaca tacaagaggt ctcctttcca
     1621 caattgctta gcagtaaacg ggaatttaca aagctaatct cttccatttt cttatctttg
     1681 ttctaaaagt cccaaatgga gcaactgtat ttacatgaat aaatttatat acttcccata
     1741 cagccgtttt ggttgcctcg tctgcccatt ttcttttgtg tgcagtgaat agatcttcat
     1801 cactcattga atcaatcatc atgttaatat catcaatatt acgattgaga atttcctcta
     1861 attctttcaa tggtagatgt gcataggtat ttgtaaacca cttatataac tctcctaact
     1921 gattccattt aaattgatca gaaggcgttt taacagataa tccatttctt tcatcatttt
     1981 cccattttag aattaaattc gtccaaccaa cttgatatgc taaattttca gcaggtgttc
     2041 tatccacttc ctcaactctt ttatccttca attcttcagg aatagtttca aattctaaaa
     2101 tatatttagt ataagtccta tttatttctt tttttaattc ttctttattt ttatatgttc
     2161 tcattatatt acctccataa attcctaatt gtctccttta attcatctat aatttcatcc
     2221 acgatatttt aatccgttct acaatgcata tctgatatgc attgactgtc taactcatat
     2281 gtgaatgatt tataaaatat cccaacgtct catctaaaga aatgttgatc gtcagcaagg
     2341 agctaaaaaa tacgaatata tttcttttgt agtaaaatcg gactggtttg catatcattt
     2401 tcttcgtaaa tatcaaaatc atgttaacta ctatcaatta ctaataaaaa ctaccacttt
     2461 aagcgttaag aactgttaag tttagttaag tgaggatagt tcacaaaatc cattgttaaa
     2521 cttcacgaaa gtggtaaaac tcaaaatgct ttatatagga ttacagcatc gctctttctt
     2581 cgattgcgcg ttgggtaaaa aatattatga agtaaaaatt gacgatgaca cgttccttac
     2641 ggctcatcag atgaaacagc tacaaaaacg aaatgatcaa ctcgaagaaa atttaattct
     2701 aaaaaagcga ttgccatatt cacgcctcgc tcgacgaact attaaaggtc aagatttacc
     2761 tgaagatcat cctttttagg aaattgaagc tttttatgtt gcacaatgca tttataatac
     2821 tacaatgatg ttttcaccag atattcttct agggtgcggt gtaatgaaac agatacactt
     2881 gcaaaagaaa attcaccgcg aatttactaa aattatgaat aattatgtta atcttccgaa
     2941 atttttttcc aaaattaagg gatcaagctg gtgttataga ttgtttaagg ttggcaaaaa
     3001 aataacttct ttttaaacaa ttatagaaaa ccgatactct gttaagttgg ttgacacggt
     3061 gtcgattttt atgactttct tattctgaaa cttcactaac ggacagtacc taaaaagtct
     3121 atagtatgga cgtttacgag tactatttta tcaaaaatgc tgctcatttc aacttatatg
     3181 ttttagtgaa ctacccccac ttagc
//
LOCUS       DAJSMI010000205         3203 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJSMI010000205 DAJSMI010000000
VERSION     DAJSMI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.209"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            26..238
                     /locus_tag="ON430_002596"
     CDS             26..238
                     /locus_tag="ON430_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220478.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(516..2315)
                     /locus_tag="ON430_002597"
     CDS             complement(516..2315)
                     /locus_tag="ON430_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR3220479.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2439..3035)
                     /locus_tag="ON430_002598"
     CDS             complement(2439..3035)
                     /locus_tag="ON430_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3220480.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1143 a          590 c          572 g          898 t
ORIGIN      
        1 aaaaataaag aaaagaggga atgaaatggt aaaagcagaa gtaggaagat cttatatatg
       61 caaaccgatc ggagtatctc atgaactaat cggctgtatt gaacgtacat atatcaacac
      121 ggctgtcgtg tatgttgaaa gttatgacca acgagactat caacttatca atgaaagcaa
      181 gtatcgcatg ctcgtaaaat tctgtgatat tagcgcacct gctgaattgg tttcttaaac
      241 agtatttttg agaattcgca agaactccct tataatataa ataaagacag aagggagcga
      301 gaagatatgc caaaaagaaa aagttcaggg aaaatgggaa ttattcttgc agtgatctct
      361 gcaattcctg ctattttagc tattgttcgt gcgatgaagg aatcaagaga aagttaagca
      421 cacattatga gcgtcaaaca aaaaacgcat atacctgcgg tggcaggtta tgcgtttttt
      481 gttgtataaa gacttttttc attaacgatt tttgattagc cgactcccaa aacaaaattt
      541 agcagtttat ctgaataagt gatttcttca tctgtttgtt tcacactttc atttagtagt
      601 cccatctcat cggttatgat accatccaca ccataaaaca tcatgcgact catagcatct
      661 gaatcgttga ttgtccatac atatacttct tttccgtctt ggtgggcaga attaatgaag
      721 ttacggttga tcgtactata ttccattgtc agaaaatcag caggcgtcac agggggccca
      781 acaatattga aaggtaagat gtagcccaca taaaagtttg gttctgcttc ttttaaatct
      841 gaaacggttt ggtacgtcaa actttgcaaa atatgatggt tctctaagat aactttgcgg
      901 tatttatgga caaatcgttc gacaaggtca tccgaatctt ttttagaggt cttgatctca
      961 ataagtaatt tttggtttag cttatgtgct ttttccaaat aagaatcaaa agaacaaaca
     1021 ggggcttgca ctccgttttc agtaactgta agatttgtca attctttcaa tgtaagatcc
     1081 tgtggtgttc catttacccc tgttaagtga cgaagatttg catcatgcat cactacgaaa
     1141 tgaccatctt ttgtttcctg aacatccatc tctacataat caggatgata gtctctgctt
     1201 gttttttcaa gtgcttctaa agtattttgc gccccatttt gggtactcac acctctgtga
     1261 gagacagtca ctggttttgt ctgaacagct gaagtcagat aatccatatt gtaaagacag
     1321 acgccaatac cgaaaaaaac tgcaaaaaga gtcaaaccag tgtttttcag cgcagagaaa
     1381 cgcaagttgg gggcttgtgg gataaaccac ttagggatct caggcaagaa gccttcgtca
     1441 tccatgaaat caacaatgat ataaaaaatc cctacggtag acatcacgat attgaataat
     1501 aaaatccctt gtaaaagcgt catagcaaac acagcgctga tcaatgcata gtctgggaaa
     1561 aattgttcaa ccatactttg tgccaaaata acaacgatat atcctaaaga agaaaggagt
     1621 aaaatcgtac cgccaatcac aataaattgg cccgtgatgg ccaaaagacg cgatttggtc
     1681 aatgaccagc tttcccgaat ggctgctcga aaaggacgat cccgtagaat catttccggt
     1741 aaagcaaaaa tcagtcgaat cccaatatag ccaaggaaaa tataaacgag taaaaaggaa
     1801 ctgacgatga tccaacgatt agcaaaaata aaatccataa tgaaagcggg gatttttatt
     1861 tttgacagta gatcagaatt aaaacttaat ccgctgatcg gtagaatcaa taaaaaataa
     1921 gcaaggaaaa acaaaaaagt aatgggtctc actttcttca gctgaagtat cgtcaagtgt
     1981 aagagttgct tcaaagagat tggttctttc tttttaataa aaaaaacact catcaaaaga
     2041 aaagtaaatt cgaaataaac taacagtaaa atcgtcatca aaactacgat taacgagagt
     2101 agaaccccag gatgttgaga aaaaattgaa ggaatattgt cataagacaa atagtcaatt
     2161 gcccctcggt gtaaaataaa acgggtcgta ctgctcaata agggtaaaca aataaataaa
     2221 ataaagccat gcatcagcaa gacatcacgg aaataagctt gtgttccttt taaaaagtcc
     2281 caagcattac ctaagctacg tttcaaataa ttcatcctat cacctgctct tcacattata
     2341 gcgcaaatct aatgaaaaag gaaagggtgc agtgtgtttt gcagaccctt tcccagattt
     2401 aacattgtca atcaatgact aattttacaa aataaatatt atccaagaag accacttaga
     2461 tattgacctc cataaatcaa ggccatacta tataagaaaa tcgacagtgg tttagcaatc
     2521 aaaatgatta gtgtatatcg tttcactgac atgtttgtca atccagccat caaacataac
     2581 gcatcgtcag gagcaactgg aagaaaaata gctagagcaa acagcttctc aaaacggttt
     2641 tgattatcca gccagccaat atatttgtta tatgtcttat cgctaatcat ggaaagtatg
     2701 aatggctttc cataacgacg ccctagcaga aaaatgataa tcgaaccaat gcagattcca
     2761 atatagttat agataaatcc agcataaggg ccaaatatca ggacaccagc tgcagtactg
     2821 atccctccag gaatgattgg aataactact tgcaggattt ggatgaagac aaaaataatc
     2881 ggtcctaaaa tgatggaatc acctactaat cctcttaacg cattcaaatc tttgaaaaca
     2941 cctaaattaa taaaataaat cgtcagcgcg attgataggc ctaatccgat aatggatatg
     3001 aaatttatca gttttcgaga ggcagcaata ttcattattc atcaactcct tagaggttct
     3061 ttgttaacta tactttacta gaaaagcaac taactgcgcc attttttgat atgacagaaa
     3121 acaaacaatt ttgtaagttc atcataagta taaaagattg atctcaaaaa aacatccaac
     3181 taaagactta tttaataact tgt
//
LOCUS       DAJSMI010000206         3154 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJSMI010000206 DAJSMI010000000
VERSION     DAJSMI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.210"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(16..708)
                     /locus_tag="ON430_002599"
     CDS             complement(16..708)
                     /locus_tag="ON430_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3220481.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1145..1714)
                     /locus_tag="ON430_002600"
     CDS             complement(1145..1714)
                     /locus_tag="ON430_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3220482.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="ON430_002601"
     CDS             complement(1788..2111)
                     /locus_tag="ON430_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220483.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="ON430_002602"
     CDS             complement(2265..3077)
                     /locus_tag="ON430_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220484.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT          874 a          636 c          553 g         1091 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacctattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccct
//
LOCUS       DAJSMI010000207         3126 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJSMI010000207 DAJSMI010000000
VERSION     DAJSMI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.211"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..88)
                     /locus_tag="ON430_002603"
     CDS             complement(<1..88)
                     /locus_tag="ON430_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCR3220485.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKK"
     gene            246..509
                     /locus_tag="ON430_002604"
     CDS             246..509
                     /locus_tag="ON430_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220486.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            533..721
                     /locus_tag="ON430_002605"
     CDS             533..721
                     /locus_tag="ON430_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220487.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(804..989)
                     /locus_tag="ON430_002606"
     CDS             complement(804..989)
                     /locus_tag="ON430_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220488.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1204..1575
                     /locus_tag="ON430_002607"
     CDS             1204..1575
                     /locus_tag="ON430_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3220489.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1725..1797)
                     /locus_tag="ON430_002608"
     tRNA            complement(1725..1797)
                     /locus_tag="ON430_002608"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1762..1764),aa:Gln,seq:ctg)
     gene            complement(1843..2271)
                     /locus_tag="ON430_002609"
     CDS             complement(1843..2271)
                     /locus_tag="ON430_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220490.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2331..3089)
                     /locus_tag="ON430_002610"
     CDS             complement(2331..3089)
                     /locus_tag="ON430_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR3220491.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1023 a          507 c          590 g         1006 t
ORIGIN      
        1 tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa aaaggaatat
       61 aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa gtccatgata
      121 caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc ggtaaaatct
      181 gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga aggagtggga
      241 ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat attcgggtga
      301 tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa ataaatggta
      361 ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat tatagtatcc
      421 cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat accaaaaaag
      481 catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa aaatgaatcc
      541 atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg atgtaaaaag
      601 agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta aatatgtggt
      661 gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac gtttatttta
      721 gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt cctcctttga
      781 ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt tttgacactg
      841 tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc taattgttct
      901 ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat catttccttg
      961 actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat caacatgatt
     1021 ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata tcaaataacg
     1081 atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa aatctccaac
     1141 ttgggacgga tggaatttct ttaactgacc catatactga atatataaca aggaggtaga
     1201 aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat tttgctattt
     1261 ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact tgatcagcta
     1321 aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga ggaagtaaat
     1381 gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa agaacgaacg
     1441 gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat tagcaataaa
     1501 ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga gaaagcatta
     1561 gcaattattg ggtaaatcag tttttgtatt aagaatacgc ccgattttat tattcataat
     1621 ttaattatgt aaactaaata caagtagtaa aaataataca ataatccccc tgcaaaaaga
     1681 ttgttctttt acagggggtt cattttttca gcaagcacat caaattggga tggcagggtt
     1741 cgaacctaca attgcttgat tcagagtcaa gtgccttacc agtttggcta catcccattt
     1801 attcaacaaa ataatcatag cactttccta aaaaaacgca agttattttt cttgatcgga
     1861 tagttgttgc tttactcgta aaaggcggcg tactagtttc ttttctaatc cagcaggaag
     1921 ggcaatcagt tctgctactg acaagacggt gtccaataac tgatattctt tgacaggttc
     1981 attcataata tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag tatactctgt
     2041 tcgagcaatt agaaatttca ataaatcggt ggctttctcg tactcttgat ctgcgtaata
     2101 aacaagagat tgacgataca atttgatttc cagttcattg agggtagcag gtctgcgacg
     2161 ctcgttccac tcattaatag agactttacc ttgaacattt cccttgcagt cacggacaaa
     2221 aataatcgga atttcatttg gatccatttt tttatgtgtg aatttttcca ttatctacgc
     2281 tcccttcaaa ggtttggtat cttgagtata agggattttt gtttgaatta ctagcttttc
     2341 tccaaagtta gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc gttgatcggt
     2401 actgctcgat tttttacacc atagggatcg ttgacataaa tattttcttt gtcatatcca
     2461 gtgactacac aagcatgaac aagaggagtg actcttactt ctccattatt tgtggtccaa
     2521 gtcataaaat cagtttcatc tgggacttca aaattgatag tcacagaagt ccagacagga
     2581 tttcctttag atagtgcttt tttgatatca tcgagttgag catcggtttt tggaaaagtg
     2641 tgataatccg tttggacgat ttgctgagca agggaggcaa ctgggggaac atagacgacc
     2701 atagcccagt ctccgccact gatatcacca acaaatcctt cattaggatc gccatgagtc
     2761 ccatctgcat tgaaaacagg gacgtaatcc aattgctcag ctaactggtt tttagttgtt
     2821 tcataaccat aaaaagataa aagcatagaa agcgaagtga cttcacagcc attttctaaa
     2881 gaaggagaat caaactggct ctctaatgga acatctagta aaacttctgc tttctctttt
     2941 gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg ttctggagaa
     3001 tggatatgta ggtaataact tgtaccgctt aataaaagag cagctgtgcc ccctagaaac
     3061 aatagttttt tgaacgtttt tttcttcatt tgaaatctcc cttttcagct gtttatcttt
     3121 cctatt
//
LOCUS       DAJSMI010000208         3122 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJSMI010000208 DAJSMI010000000
VERSION     DAJSMI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.212"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..236
                     /locus_tag="ON430_002611"
     CDS             <1..236
                     /locus_tag="ON430_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220492.1"
                     /translation="MGKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDME
                     VAVAVYSTMMFKQARDAYQKEFEKANKEKKLQK"
     gene            239..421
                     /locus_tag="ON430_002612"
     CDS             239..421
                     /locus_tag="ON430_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220493.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            439..>3122
                     /locus_tag="ON430_002613"
     CDS             439..>3122
                     /locus_tag="ON430_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3220494.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSW"
BASE COUNT         1105 a          462 c          705 g          850 t
ORIGIN      
        1 cgatgggaaa aggctctatg gatattacag ttattcaagg tgcaaaagcc gtatatgtag
       61 cttatcgtca agcgaacatg gtcgattaca tttcattcga tgaatttata gataaatggg
      121 attttgatat ggaggttgca gtagctgtat acagtactat gatgttcaaa caagcacgtg
      181 atgcctatca aaaagaattc gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat
      241 gccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg acgttttttc
      301 tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc aaatggctta
      361 caacaagagc gcttatgaag gatggaagaa ctatgcaata aatcaatccc agaaaaacta
      421 aaggaaagga ggtaaaaaat ggctaaaaag agaacggaag cagaagtaac tttcatagct
      481 aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg ctgaattaac taaaaataga
      541 gcagaattaa aactagaaca agctcaatta caacagactg gttctgaatc agacaagtta
      601 ggaagtaaat tatcttcttt agaaaagcag tatgaattac aaagtcaaaa agttgaagta
      661 actagtcaac gtttagccaa tgccaaaaaa tattatggag aaaattccac cgaagttcag
      721 aaacttgaaa gagaactgat taatcaacaa acagctcagc aaaggttatc taatgatata
      781 gataaaacga gtaaggcatt ggctcaatct aaaggtgaat ttaaaacgta tgagtcaact
      841 atgaaagact tagataatga gcaacaacat ctaaagtcaa gcgcatcatt agtagaatcc
      901 gaatataaaa aatggcaagc aactgctggc cagtctgcta cagaatctga aaaattagca
      961 aaagcacaag aatatgtcgg caaacagagt gatattgctg aacaaaaaat cgaagtgcta
     1021 aaaaaacaat tagatgccac acaaaaagaa tttggagaaa catctaccga agctttagaa
     1081 atgaaaacta agcttaatga tgcggaaaga gaattcgaag aattaagtaa tgctgctaaa
     1141 aacgttgata cctccacagt agatgatatc ggcaaaaaac ttgatatggg taatttaatg
     1201 gaagctactg atcacttgtc agtgatcgga gataagctta ttgatgtagg tagtaagtct
     1261 attgaagcag ctggaaaagc acaagctatg caggcccaat ttaaacaagt ctttggctct
     1321 ttagaagggg aagcacagga cgccgttgag ggaatggctg aagaatttgg aatgttacca
     1381 aatacgatca agcctgtttt tacacaatat acgtcaatgt ttaaaggact tggatatgat
     1441 accaaagaag ctatggagtt agctggtgat agtactcagt tagcagcaga tgcagcagct
     1501 ttttacgata agtctatgga tgatgctagt gaatctctta attcatttat aaaagggaac
     1561 tacgaaggtg gggagcaaat aggtttattt gctaatgata ctcaaatggc agcttatgct
     1621 gttaagcata atttgatacc agcgactgaa ggagcaaaaa aagccagtga agagtcattg
     1681 ttagctgttg aaaaagcaca atctaagtat gctgatgcta ttaagaaaca tggtgaagga
     1741 tctttagaag caagagaggc tgctttaaaa cttaaagatg cgcaagataa aataaatgaa
     1801 gaattaggcc cacaaacgca aaaatggtct gatttagatg aggctaccaa gcaagcagtt
     1861 cgagttcaat atgctgaaga tatgcaaaaa ttagccggtg ctacaggtca ggctagtagg
     1921 gagtctgatg gtttagaaaa ccaaatgaca agagcaaagc aagcgttaga agatttttat
     1981 gcctcattgg gtgaagatat actgcctgtg tttatcaaag ggttgcaagc aggagcgaaa
     2041 gctttgcaag gattagccga atggtggagt aaacttgatg ggccaatgaa aaatttcatt
     2101 ttagctcttg gaggaattct agcattatta agcacattag ctcctgttat aaccgcagtt
     2161 gttacgatag ttggcacatt tggttctaca gttttgctgc caataatagg aattatagcg
     2221 ggcgttgcag ctgtaatagc gattgtaatt acagcgtttc aaaactgggg cgcaatcact
     2281 gactggttta gtgatttatg gaaaaaattt accgattggt taggtgatac ctgggaaagt
     2341 ataaaagaaa gtgcctcatc agtttgggat ggagtcaaag aaacctggtc tggatttgta
     2401 gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt atggagcgga
     2461 ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca agcattcgtt
     2521 gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat ttgggcagac
     2581 attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca agctggtatt
     2641 aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc ttggcaagct
     2701 gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac acaatggata
     2761 agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc agtagtggca
     2821 tggtggaatg gtatatggac atctattagt actacggctt caaatatttg gaattcaatt
     2881 agtgcaacag cttctagtat ttggaacagt atcaagaata caatcactag cttggtacaa
     2941 gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt aggtggaatt
     3001 tggaattcaa tcagctctac agcatcaaat atctggaata gtgtgactag tagtataagc
     3061 aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat atcttcgtgg
     3121 at
//
LOCUS       DAJSMI010000209         3083 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJSMI010000209 DAJSMI010000000
VERSION     DAJSMI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.213"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(71..1006)
                     /gene="murB"
                     /locus_tag="ON430_002614"
     CDS             complement(71..1006)
                     /gene="murB"
                     /locus_tag="ON430_002614"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR3220495.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1079..1999)
                     /locus_tag="ON430_002615"
     CDS             complement(1079..1999)
                     /locus_tag="ON430_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3220496.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2112..2864
                     /gene="xth"
                     /locus_tag="ON430_002616"
     CDS             2112..2864
                     /gene="xth"
                     /locus_tag="ON430_002616"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR3220497.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          872 a          626 c          534 g         1051 t
ORIGIN      
        1 taaacaacca caaaaaagag caggacggcc accgtcctgc tcttttactt attttaagtt
       61 ccattactta ttaattggct aaacttctgc ttgtttctga tattgcttgt ttttctccaa
      121 tgatccgcac ttctgtttct aaagacacat caaatttttc tttgattact tcttggatgt
      181 gcgcaatcaa ttcaatataa tctgtcgctg tcgcatgatc gatattgacg atgaatccag
      241 catgtttttc agagacttgt gcaccgcccc atttcaggcc ttgcaatcct gcttgttgga
      301 tcagctgacc tgtatagtgt cctactgggc gcttaaagac acttccgcat gaagggtatt
      361 ctaatggctg ttttgattga cgcaattcgg tcaactcatc cattcttgct ttgattgctt
      421 cataatcccc tgtttgcaaa ctgaatcgag cttccaatac gatagcacgt aattcttgga
      481 ctttactgtg acgataagaa aataccattt cgtcttttgt caaagttttc aatgttccat
      541 cttcaagcaa aagatccact tcagcaaaaa catctttgat ctctccgcca tacgcacctg
      601 cattcatata aaccgctcca ccgacacttc ccgggatgcc gcaagcaaat tcaaatccag
      661 tcaatgattc tgccaaagct gcataagtcg tatcaatcag tgaggcacct gcttccgcaa
      721 tgaccatcgt tcctttgaca tgaatctgtt tcatttcagt cagcatgatg actacaccgc
      781 gaatcccgcc atcttgaacg atcagattgc ttgcatttcc taataccatc caaggtatat
      841 catggattcg gcaataatcg atgatttgct ttacttcttc ttgttttttc gggaatgcaa
      901 ggacatcagc tggtccacct gttttcgtaa acgtatagtt catcaatggt tcatcaaaca
      961 atatagtaat cgtggctaag ctttcaacta tttcttcttt attcactgag gattttcctt
     1021 tcttttctca aacgtcctca ttattctatc acgcttaata caatcttgcc agtcttgttc
     1081 aaagttgatg acggtagtga tctttaagca cttcctcaca taattcatga cttcttacaa
     1141 cttctttttg tttaagcaat tctgtttccg atttacctaa acggatctga gaaaagaagg
     1201 cctctaccat tggctcaaac ccgcgtttga ctagcgtgtt tgtccagtcg ccaaattctt
     1261 tcactttttt tccatcagca tcttgaatga caagctgggt cagatcgttt acttcataag
     1321 taccattttt acttgttact tgatattttt ctatattagc gccacttgct aaatccatcg
     1381 ttaaaatagc cgtttgattt tgtgtttcta attgtaaaat ggcatattcc aaaaattcct
     1441 ttgtttctct tatctggtat tttgttctca aaatgggttc atctaataaa tacacagctg
     1501 tatcaataag atgtaaaaac aaatcataaa ggacgaactc tgtcgtttct tttgccgcta
     1561 ttcgattttt ttgcaggtgg atcaaccgct tatctgggat acgtttcaat tcttctacca
     1621 taggagcaaa gcgccggttg aaaccgatca tcaaaattat ttggttctta tcagctaatt
     1681 cttgcaattc acgaacttca tttagatgga cactcagtgg tttgtcaata taaacatgga
     1741 taccattttc tagacatttt ttggctaatt cataatgtac ttttgttgct gcgtggatca
     1801 tacatgcttc gattttttct tctaaaagct catcgatcgt ttcatataga tgttggaacc
     1861 cgtactcttt ttgaattctt tcttttgttt ctttatttct tgttgcaaaa taaaaatcaa
     1921 gtgttccttg ttttttcgca taagttggta aatatgcttt ttctgcgata ttccctacac
     1981 cgattacacc aatcttcatc tactttcctc ctggatcatt tagttacttt catcttacac
     2041 cattttttac taaaatgagg tacaatagta tcgcaaaggt aaggaaatta attcgaagga
     2101 gtgtgctttt attgaaattt atctcttgga acgtaaacgg gcttcgtgcc atcgtaaata
     2161 aaaatttttt agaagttttt catgagttag acgccgattt tttctgtctg caggaaacga
     2221 aactccaagc aggacaaatt gatttagatc ttccaggtta ttatcaatat tggaattacg
     2281 cggaacgaaa aggctattcc ggcacagcga tttttgctaa aaaaccagca ttgaatgcca
     2341 cttacggaat gggtatcgat atccatgata cagaaggccg tctgattact ttggaatatt
     2401 ccgatttctt tcttgtaacc tgttacacgc caaattctca aagcgaatta aaacgtttag
     2461 attaccgatt agaatgggaa gaagcctttt ataattattt ggaaaatcta aaaaaacaaa
     2521 aaccagtcat cgtgtgtggg gatctaaatg tcgctcatca aaagattgat ttgaaaaact
     2581 ggaaaacgaa tcaaaaaaat gctggattca ctccagaaga aagagcggct ctttctcgac
     2641 tgttggataa cgggtttatc gatacattcc gttattttta tccaacccag gaaggtgtct
     2701 actcttggtg gaactaccga ttcaattcaa ggaaaaacaa tgctggttgg cggatcgatt
     2761 actttttgac gagcaaagat ttagagccag gactagctga tgcaaagatc catacggaca
     2821 ttatgggcag tgatcattgt ccagtagaat tagatttgaa ataaagaggt tgtgaaaaag
     2881 tcgtttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca ttttgcataa
     2941 atttggcata ataccgagaa actggctttt gaacactgtt tatcgagaga ttgtgaaaaa
     3001 gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat atttttcgtt
     3061 tggttgggct tattaccgca gat
//
LOCUS       DAJSMI010000210         3081 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJSMI010000210 DAJSMI010000000
VERSION     DAJSMI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.214"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(174..1484)
                     /locus_tag="ON430_002617"
     CDS             complement(174..1484)
                     /locus_tag="ON430_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR3220498.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1496..2914)
                     /locus_tag="ON430_002618"
     CDS             complement(1496..2914)
                     /locus_tag="ON430_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3220499.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          972 a          629 c          474 g         1006 t
ORIGIN      
        1 aagtggttct ttttaacgac ttagttatct tatcacgtct aaaaacatgt gtcaactctt
       61 ttttggaatt ttcataaaat gtttcaatga agcgcgacaa cgttgtttaa tatatcatta
      121 aaaaaaataa aggtcaagct aaaatattat ttaagcttga ccttattata atttcaacga
      181 ttgtcaaaag tattgtttgc agctgtgttg ccagtttcta tagataaatc acagcttttg
      241 aaacataagt tttgtactgc ttcgattcgc ccgaattctt ttgcagttat ttggcaatga
      301 gaaaagcaga catcttctat aggcttttca ggaaatgcta ctaaatcaaa agctcgggaa
      361 ggaagtgagt aacctggaga aaggcatgcc tgtacgtttt tgatgtgtat gtctttgact
      421 ttcgtcattt gctttgattc aggtatttct tcagccactg cttgccaact ttctggcatt
      481 tgagcaaggt cattgctatt ttttctattg tattcattat gccagtccat tatccaagaa
      541 aaaggaaatt gtacatcaat catattcaga ttttctaccc tgatattttc gatcactccc
      601 cctctttctt ttgaagattt cattcgaata ccacagtctg actgaaaaaa atcgatatcg
      661 tgaatatata cgtcactaac tccagcacta acttcgctac caatagttac accgtatcct
      721 gaacggattt tacatctagc aacctcgatt cttgatgatt ttcgattgac tcttgcacca
      781 ttgccatctc tgccagactt aatagcaata caatcatcac cacaggccaa atcacattca
      841 tagacacgca catttgttga agaatcaata tcaatcccat ctgtacttgg tccatcattg
      901 ttgcggatga ttactttttc tactaataca tcgttagaat aagtgatttg caagttccaa
      961 aaacctgatt tctgaaaaat caaatcacga acaacaacat gttctgcatg atacagtaaa
     1021 caagcgcgtg gacgtttaat agcataatcc gcaatccaac gaagtccctt ttgatcgtat
     1081 tcagctcttg tcccgctttt ttgatctttt ccccaataca gctcccacca atggtctcct
     1141 tgaccgtcga ttattccttt tccttctata agaatatctt tcgccgagag aacattcaaa
     1201 atcgctgctg gccatttcat ttctacgccg gctactcgtg tatcgatttc tggaaatgct
     1261 tcgatatctg ttctcccctt gaggactgca ccttcttcaa agataagatg actcttatct
     1321 tttaaaaaaa gggctccgac taaataaatg ccttttggaa taaatacagg taagtgttct
     1381 ttcgcacctt gatcgattgc tttttgcagt attattgtat catcttgttg agtgtcgttt
     1441 gttttaaagt tcaaagcatt gataatccct ttttttgtcc gcaatacgtt tctccctatc
     1501 tttcattgat ttttttcaat tcttcaccaa taaatacagt ttttgaacga ttcagtgggt
     1561 aacctactag caaaatgatt gcggatacaa ataagcagaa tgctggtaac aagtttgcca
     1621 tcgtataaat cctttcttgt actaaaactg attggatggc tccacctgtt gtagacgatt
     1681 ggtaaccaat gagtgccagc ataaatccgc ctaccgctcc tgcacatgct tgtcctactt
     1741 ttctcgcaaa agaatttact ccatatactg ttccatcttc tcttaagcct gtcttatatt
     1801 gatgataatc aatcacgtca gtaatgaatg cccagaccat caaattgaat aaaccagatc
     1861 ctaaagtagc gataaataaa agaatcagat atacccatgc atttgttata tgaaggaaat
     1921 acatcagcaa gtacatacat gaagaagcaa gtaacgcaaa tacgcttgct tcctttttac
     1981 caaataattt tgtcagcttt gtagcaaatg gtgctaaaac tactactgtt ccgtaagtaa
     2041 ataaaagagc aacagacatt gcttgtttat tatggaagta atcattgaat aaataagtca
     2101 tattcgtccc agctaaaatc tgattgatca caatgaaaat gtcaacaatg actaaaataa
     2161 tcagtgctcg attagctaac aaacctttta ccaactgaga agcagatact ttttcgtttt
     2221 tgacaactct tactctttca gtagtcaatt tacaagttaa catatatgcg acaaatgcta
     2281 ttgcagcaca tccgattgaa atccaaaaga aatgattccc tgaaacgact tgttgtccac
     2341 tcttgttatg ggtatacatt aaaatcggaa tgaagaatcc tgtagttgat ccgccaatcg
     2401 cagaaccaat gcttcggaac gtggacaacg aggtgcggtc attaggattt gagctaattg
     2461 ctgctgccat tgctccatac gggatattga ttgttgagag aaaaatccca taaacaatgt
     2521 atgaaacaaa aatataagca atcttcaccg tcattggaag atgttggacg aatggcaaaa
     2581 acaaaatgac agtcactaag caaaatggat acttcatttt tctgatccaa ggaataaatc
     2641 ggccagtttc tgttaactct gagacatcac ataatcttcc aaccatcaca tcagcaaacg
     2701 catccaaaat tctcgaaatc aagaataacg tacctacgat tgcccctgaa acgccaagaa
     2761 cattcgtata ataaatcatc aagaaactat tgactaaacc taaaataaaa cagttaccta
     2821 gatcaccgaa catgtagcct atcttatcgg aaatcccaaa ttcctttttt tcttttttta
     2881 ttgtatttgc tgtaacagca ttttgtgaaa gcatttgaat tcctcctacc tcttctagta
     2941 aacgttttca aaattataac tctttttttc ttgtttttat attcacaatg ttggcttttt
     3001 attgcattat cttgacagat aaaaaacaaa aaataatcta aaaacattga tgcataagat
     3061 tgataacttt ttttacataa c
//
LOCUS       DAJSMI010000211         3018 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJSMI010000211 DAJSMI010000000
VERSION     DAJSMI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.215"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..447)
                     /locus_tag="ON430_002619"
     CDS             complement(<1..447)
                     /locus_tag="ON430_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR3220500.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGG"
     gene            564..634
                     /locus_tag="ON430_002620"
     tRNA            564..634
                     /locus_tag="ON430_002620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:596..598,aa:Gly,seq:ccc)
     gene            995..1720
                     /locus_tag="ON430_002621"
     CDS             995..1720
                     /locus_tag="ON430_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR3220501.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            1975..2241
                     /locus_tag="ON430_002622"
     CDS             1975..2241
                     /locus_tag="ON430_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCR3220502.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            2238..2669
                     /gene="ruvX"
                     /locus_tag="ON430_002623"
     CDS             2238..2669
                     /gene="ruvX"
                     /locus_tag="ON430_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR3220503.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            2694..2999
                     /locus_tag="ON430_002624"
     CDS             2694..2999
                     /locus_tag="ON430_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCR3220504.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
BASE COUNT         1038 a          516 c          634 g          830 t
ORIGIN      
        1 tcctccaatg actaaagtca caggtaaatc ggtcatcttt ccgccaaata acaccataat
       61 tgttccactg atcactgccg cgctaacgat ttgcaaccag acaggaaaga aagaacggtc
      121 tcggtcgatt gcttgcagtt cgcagtaaag ttcttctaat gttagctctg aagcaacata
      181 tttacgtgaa agatcattta cttttactac tttttctaaa ttaatcgaac ggttggtaat
      241 ttgttccatt cggatggtcg aggtacgatc aagaccaata aagagaccgg tttgagtgac
      301 atagctgacc aaacggaaat ttccagaagc taaagcaatt cgactcatcg tatcttctac
      361 ccgatacatc tccgcattgc tttccatcat gattttccca gcgagtaaac aagtgtccag
      421 caacagatct aattcttttc ccgtcatagc atcttctcca ataattgatt gattagtttt
      481 attttactac aaaacatacc agaagcgtaa agaataaaaa aacttgagga aggaataaaa
      541 gaggcgtata ctgtttttgt attgcgggtg tagtttagtg gtaaaatccc agcttcccaa
      601 gctgatgtcg cgggttcgat tcccgtcacc cgcttaaaat aggttgtgaa aaagctgctc
      661 tgcaacaagt aataagaaca gccaagcaaa aacaacttct tatgttttta acttggatgg
      721 acttattacc gcagatgcaa gcttttgcgc accgtttacc aagcggttgt gaaatcagtg
      781 tattgtcttg agtatgaaga aaatttttga ataataataa ataccttaca gatagagtgg
      841 tgactgatta gagacgagga atccgtctct tttttgtatt tttaagccat cttaaaacag
      901 gttttatttt ttgctgtttc tatttctttc tagtatttat ttatagggga acatatgtta
      961 agatttaggg agctgttagt aaaggagtaa agaaatgagc aaaaaaacaa ccaaggcgat
     1021 cgtggggatc gtcgctgctg tgattgcagg ggcattcggt gtttccacca cacaaaaaga
     1081 ttcagatatc tttcagcaga ttacaactat ttttgattcc acaccacaag cttctcaagt
     1141 agcggatgtg ccagaatatg atggaaaaca tcaagaaata gaaatcaatg ggaatcagcc
     1201 ggaatttaca aaagaggatc ttagcctttc caaaggaaca tgggaaagct attcaaatat
     1261 tgataaattg aacagagtag gacaagccaa tgctatgctt ggaaaagatt tgttccctac
     1321 tgaaaagaga gagcctttgt atattgaccc gacaggttgg aaacaaaaga aattaagtga
     1381 cgggcaatgg ttatataatc gcagccattt aattggtttc cagctgactg gtcaaaataa
     1441 caacattaaa aacttgatga cagggacacg ttcgttgaat gctccataca tgcttgctca
     1501 cgaaaatgat attgcggcat atatcaaaga aacaaatcat catgtacgct accgtgtcac
     1561 tcctcatttt gaggggaatg aactggtagc caggggagtg caattggaag cagaaagcat
     1621 tgaagacaaa aaaattgaat tcaatgtctt tatctacaat gttcaagatg gctacacgat
     1681 caattatgag acaggacaag caacaaaaac aaaaaattag tagcagctat tcgaattttt
     1741 tatgaatatt atgaaaaaat tgtgtaaaaa catacattca aagaaatata gcgtatactt
     1801 tatgaaaaga gtacttttga taaagctaca gaaaaaagga aaaaaacata caaatagtgt
     1861 ttcttaattt attcggaagt ttggaatatt tgtttgtagt tttccctttc ttcgagtaaa
     1921 ataaggttgt agaaggtaag ctggaattac cttgcgggaa agaggtgtag aatcatgggt
     1981 tttacagatg aaaccgtacg ttttggtttt gacgatagtc gcaaaaaaga agtgagcgaa
     2041 acattagcga ttgtttatcg ggctttggaa gaaaaaggct acaatccaat caatcagatt
     2101 gtcggctact tgctttctgg agacccggcc tacattcctc gttatcgtga tgctcgaaat
     2161 ttgattcgcc gtcacgaacg cgatgagatc atggaagtaa ttgtcaagga ctacctctca
     2221 aatcatggag tgaatttatg agagtaatgg gcttggatgt cggttctaag acagtaggtg
     2281 ttgctatcag tgaccccttt ggctggacag ctcaaggagt agagatcatt cggatcaatg
     2341 aagcagaaga agagtttggc tttgatcggt tgaaggaatt agtagcacaa tatgaagtag
     2401 accggtttgt agttggatta cctaaaaata tgaacaattc catcggacca agggcagaag
     2461 cttccatgtc atacggagaa aagatcaaag aactattctc cttgccagtt gattatcaag
     2521 atgaacgatt aaccactgtt caggcagaac gtatgctagt agagcaggca aatacttcaa
     2581 gagccaaacg aaaaaaagtc atcgataaat tagcagctgt gatgatctta caaaattatt
     2641 tagacggctc aggtaagcaa ttattttaat aataaaagaa aagaggtaat cttatgacag
     2701 aacataacca ttcccacgac catgaaggac acgaacacat cacattggtg gatgaccaag
     2761 gaaatgaaac attatatgaa atcctactaa cagttgacgg acaagaagaa tttggacgta
     2821 actatgttct tttatatcct gctggtgttt cagaagatga agatgtagaa ttacaagctt
     2881 atgcatacat cgaaaacgaa gatggaacag aaggcgagtt agaacaaatc gaaacagacg
     2941 cagaatggga catgattgaa gaagtattca atacattcat ggcggaagaa gaagaataga
     3001 ctataaagct aggagaaa
//
LOCUS       DAJSMI010000212         2947 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJSMI010000212 DAJSMI010000000
VERSION     DAJSMI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.216"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..1235)
                     /locus_tag="ON430_002625"
     CDS             complement(<1..1235)
                     /locus_tag="ON430_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR3220505.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQK"
     gene            complement(1228..2820)
                     /locus_tag="ON430_002626"
     CDS             complement(1228..2820)
                     /locus_tag="ON430_002626"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR3220506.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
BASE COUNT          823 a          597 c          472 g         1055 t
ORIGIN      
        1 atcttttgta agaagccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga
       61 gcgatcgtgt cgtcgagttg tttgaagaaa gcgccgattt tttgttgttc ctctttattc
      121 ctagtaaaag gaaacttgat attagcgaaa ttctcataga ataagtattc tcgtacgctt
      181 ccttcagtgt tgcgcttgac ctctttgata aatgttggtg acttgataaa ctgaagtatg
      241 aactcattat ctaagtcctc gctcattttc acaactacat aaagagagct aacaatgacc
      301 gtcataccaa gattgttaaa tgcaattgat cctacgttta ctcttgctgg attataggcg
      361 aattcatttg gttcaacgaa tttataattt gttttttcta aatcatccaa cctactacca
      421 tcaaactgtt ctgtttgaga aattaaacct gctttattac ttaccgaata ggcagggaag
      481 tctaaatctt tcgtgttctt ttttccagtc ttcttactaa actctttgaa cttacgctgt
      541 tcccaatctt ccgtaaatcc aggaaaccga atttctggca cttttgctcc gtttttaggg
      601 aacatttttt gtaagaatcc ttttttcgtt tcctttaata aatctagctt acgttgttga
      661 agagcgatag tgtcgtccag ttgtttgaag aagttcccaa ttttcttttg ttcttctaat
      721 attggaaatt gtactatcca ttttttcact tctgacattg gtacgagatt tgtaattgct
      781 gaacctggat tagctcctgt gagttttctt tgcataaaat ctgcacgaat aaattgaata
      841 aaatattctg aatctgtgat ttctttattt attctaagtc taatcaaagc ctgattgaat
      901 acacccggtt ttatttgctt tggtactctt gaaattctcc caattgttcc agcgccactc
      961 atgataaaat cgcctggttc taatttaaat ttgattaatt cattatattt tttttcagta
     1021 attttatatc ttgtttcgta attatcataa atagcatttt gttgttcata gacaacaaaa
     1081 ttgctattag atacaaagaa ttctttcttt aaggcactcc caaatggtcc cctccttact
     1141 ccatcttttt tatcaagtaa gcattcaaac ttacgctgtt cccaagcttc cgtaaatcct
     1201 gggaaacgaa tttctggttg tatatcatta ctcatgtttg aacacctcca gcgcaccttg
     1261 taaccacgct tcattttcag gtgttgtttg cagggaagag atagcttcta aaagttcttt
     1321 ttctagcact tgctgttctt gtcgtatctt cttgatgtca tttccgacat gaaccatatc
     1381 aattggttct tcttcttcaa acgtatctac atagcgtgga atatttaagt tatagtcatt
     1441 ttctttgatt tcatcaaagt ttgccaaatg agcgtatttc tcgacatctt ttctttcagc
     1501 ataggtttcg aggatttttt gaatgttttc ttccgataat ttattctgat tttttccttt
     1561 gacaaattct cgactagcgt caatgaataa tacgtctcga gtttggcggt tctttttcaa
     1621 tacaatcacc gtcgttggaa tcgatgtccc gaagaacaag ttcgcaggca taccgatcac
     1681 agcataaata ctgccgtctt ctagtaattt ttgacgaatg accccttctg cagcgccacg
     1741 gaataaaact ccgtgtggca agacgatggc catagtccct gtttccttca aatgatagaa
     1801 accatgaaga agaaacgcaa aatctgcttt ggattttggc gctaattttc cataacgatt
     1861 aaaccgagaa tcgtctaaga aggtcgtatc tgctgaccaa ttagccgaat aaggtgggtt
     1921 cattactact gcatcaaatg tgtaaggttc atctgttggc caatccttat tcaacgtatc
     1981 cccattgcgg agattcattt cttcggcatc taccccatgt aaaatcaagt tcatttttgc
     2041 tagattgtat gtcgttgtat ttaattcttg cccatgatat ttaacgttat ctgggtgagt
     2101 taagtagtta cgcacattta acatcaatga acctgaaccc atggttggat caaaaacact
     2161 aaagaatcgt cgttcttttt gatccaacgt cacgatttgt gccatcatat cagaaaccat
     2221 atgaggcgtg taaaattctc cggctttctt ccctgcttct gaagcaaatt gactgatcag
     2281 aaattcataa gcatccccga tcacgtcccc atcgtgacct aaaacttcca cgtcattcaa
     2341 tttcttaatg acttcggtaa tcgtcacatt tcgttgttgc tcatctgttc ctagtttttt
     2401 tgattggaga tccacatcat caaataaccc attaaattga ttgtaggtag atgccaattg
     2461 cacaaaggct ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa
     2521 gacgttgaaa agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat
     2581 cagatcttct tttgattctt catctgaaag taattcgcga tacaacatcg tttgtttgct
     2641 taccgtatca tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg
     2701 gtcggataaa tatttataga aaatcagtcc caataaataa tttttatatt ctgacgcatc
     2761 cattttgcta cggagattat ctgccgcact aaataatttt tgatttaatt ctgcacccaa
     2821 aaagatacac tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc
     2881 ccttttagaa aaagcaaaaa aaaaaagaaa gaaacttatt cttgctaata aactacgcga
     2941 aaggagt
//
LOCUS       DAJSMI010000213         2928 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJSMI010000213 DAJSMI010000000
VERSION     DAJSMI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.217"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(34..1224)
                     /locus_tag="ON430_002627"
     CDS             complement(34..1224)
                     /locus_tag="ON430_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3220507.1"
                     /translation="MNYFTTSLMKNLFSNDNNLPAFEQAVTELFRSELEKSLNEILAY
                     ELTDFLDYEPYARTNNENSRNGSYQRKFDTKYGTITLNIPRDRMGEFFTSLLPKYRRR
                     DLFTETTILDLFEEGMSHSEICSMIQKLCGARYSKQTISNITDKALECVDSFKNRILN
                     KEYAVVYLDGTSMPLRRDTVSREMVHIALGITVEGTKEILGYMIAPNESAEIWEELLK
                     DLKSRGVERISLFCTDGLSGMENVIQDIFPGSKIQRCLVHIQRNICAKVRVSDRKEVA
                     NEFKEVYRSKSKEEALTSLKKFIDRWKHKYPSMVKSLSNNDYLFTFYDYPESVRHTIY
                     STNLIEGNNKQLKRSFKKKEQFPTEKSEEKYLVIQFNRYNEKNMNHVHRGFGQTTRED
                     WFKS"
     gene            complement(1623..2924)
                     /locus_tag="ON430_002628"
     CDS             complement(1623..2924)
                     /locus_tag="ON430_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3220508.1"
                     /translation="MENSAFIMKLNDILSPIAAKIGNQRHLKAISSGMMFGLPFIVIG
                     SFFLIFANPPINIDKYNPDTANIFMKFMASWKDFAVANYDLITAPYNLTMGIIGFISV
                     FGIAFSLANEYKINPSMNGMVASVIYLMVCTPVEEGAINLNFLGTNGLFVAIIIGLLV
                     VEINRLFEVKNIKLKLPDTVPPMVATFINMLVPLLTNIFIFYGINLIFLAATNKIFPE
                     AVMEVLTPATNIAGSLGGFLLIVTLGNILWLFGINGSSIIFPIVFTLGMAQTGLNAEQ
                     LANGETMTHLMNLQMFRISVLGGAGGTLGLVLLMMRSKVPEYRTIGKLSLVPGICSIN
                     EPVIFGLPIVFNPILAIPFLITPIISLLLTYFAQQLGWIGIGFIVDPSFTPFFAQAYL
                     SSMDWRNILFCLLLIVISIFIYYPFFKVMESNKTKLVDEKL"
BASE COUNT          902 a          521 c          529 g          976 t
ORIGIN      
        1 ttttgtgtaa aagtatatcc ttggtaaagc agattagctc ttgaaccaat cttctctggt
       61 tgtttgtcca aaacctctat gtacgtgatt catgtttttc tcgttgtaac gattgaactg
      121 aatcacaagg tatttctctt cagatttttc cgttggaaat tgttctttct ttttgaaact
      181 tcgttttaat tgtttattgt ttccttcaat cagatttgtt gaataaatcg tatgcctaac
      241 actttctgga taatcataga atgtaaacag atagtcattg ttgctgagac ttttcaccat
      301 tgatggatat ttgtgtttcc acctatcgat gaatttcttt agagaagtta aagcttcttc
      361 ttttgatttg gaccgatata cctctttaaa ttcattggcc acttcttttc tgtcactgac
      421 tcttactttt gcacatatat ttctttgaat atggaccagg catctttgaa ttttagagcc
      481 cgggaaaatg tcctgtatta cattttccat cccagacaat ccgtctgtac aaaataaaga
      541 aatacgttca acccctcttg atttaaggtc tttcagaagt tcttcccata tctcagcgga
      601 ctcgtttggt gcaatcatat agccaagaat ttcttttgta ccttctactg tgattcctag
      661 tgctatatgg accatttccc tggatacagt atcacggcgt aaaggcatgg atgttccatc
      721 aagatataca acggcatact ctttgttcaa tattctgttt ttgaaggaat ctacacattc
      781 cagagctttg tccgtaatat tagatatggt ctgtttagaa tagcgagctc cacataattt
      841 ttggatcatg gaacaaattt ctgaatggct cattccttct tcaaataaat ccaaaatcgt
      901 tgtttcggta aatagatctc gtcttctgta tttaggcaga agagaagtga aaaattctcc
      961 cattctatct cttggaatat tgagtgtaat agttccatat tttgtatcaa actttctttg
     1021 ataggaacca tttctagaat tttcgttatt ggttctagcg tatggttcat aatcaagaaa
     1081 atccgtcaac tcatatgcga gtatttcatt gagagatttc tccaactctg aacgaaacag
     1141 ttcggttaca gcctgttcaa aagctggaag attgttgtca ttagaaaata ggtttttcat
     1201 taaagatgta gtaaaatagt tcatgggtat attcctttcg taaattgttt ttggtcgaac
     1261 taattttact aaggatatac ctttttttct acatttctag aaaaacgaat ttacacaaga
     1321 tattttatgc tatccattta tttttgaaac tccatttgtt ttactacgtg tattaccttt
     1381 atttttcttt ctatttttag gtgtctatta tctcttacgt tcgcttataa atgatgactc
     1441 aaaatttaaa gcaaaataaa aataaaggaa gtagcctttt gaacaagggc gcttccttta
     1501 tttgttgtgg gacaccagag tatacttttt tataaaaaat aggttgatgg gaaaccctaa
     1561 gatttcccat caacactaaa tagtatagaa ccatattttc gttacaaggt taagaatgac
     1621 gactatagtt tttcatctac taattttgtt ttattgctct ccatgacttt aaaaaatgga
     1681 tagtagatga agatgctgat gacgatcaac agtaggcaaa agagaatatt gcgccagtcc
     1741 atacttgaaa gataagcttg tgcaaaaaaa ggtgtgaagg aaggatcgac gataaatcca
     1801 atcccaatcc atcctagttg ctgagcaaag taggtcagca ataatgaaat gatgggcgta
     1861 atcaaaaatg gaattgccaa aatagggtta aaaacgatag gtaatccgaa aatcactggt
     1921 tcgttgatgg aacagattcc aggaaccaat gacaattttc caattgtgcg atattcgggt
     1981 actttgcttc gcatcatcaa taaaaccaaa cccaaagtac caccggctcc accaagtaca
     2041 gaaatacgga acatttgtaa gttcattaag tgtgtcatgg tttcaccatt tgccagttgt
     2101 tcagcattca aaccagtttg agccattccc aatgtaaaga cgatagggaa aataattgac
     2161 gagccattga taccaaatag ccataggatg ttacccaaag tcacgatcaa taagaaacca
     2221 cctagactgc cagcgatgtt cgttgctgga gtcaggactt ccatcaccgc ttcagggaag
     2281 attttgtttg ttgctgccaa gaaaatcaga ttgatgccat aaaagatgaa tatatttgtc
     2341 agcagtggaa cgagcatatt gataaaggtt gctaccatgg gtggaactgt atctgggagt
     2401 ttcaacttaa tattttttac ttcaaatagt cggttgattt ccactactag caagccgatg
     2461 ataattgcga cgaacaatcc gtttgtccca aggaaattaa gattgatggc accttcttcc
     2521 actggtgtac aaaccattag ataaatgaca gatgcaacca tgccattcat ggaaggatta
     2581 attttgtatt cattggcaag ggaaaatgca atgccaaaaa cactaatgaa tccgatgatt
     2641 cccattgtca aattataagg ggctgtgatg agatcataat ttgctacggc aaaatctttc
     2701 cagctggcca taaatttcat gaaaatatta gctgtatctg gattgtattt atcaatattg
     2761 atgggtggat ttgcaaaaat cagaaaaaaa gaaccgataa caataaaggg taggccaaac
     2821 atcataccgc tggatatagc ttttaggtgg cgctgattac ctatcttggc tgcgatgggg
     2881 ctaagtatgt cattcagttt catgataaac gcagaatttt ccatataa
//
LOCUS       DAJSMI010000214         2908 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJSMI010000214 DAJSMI010000000
VERSION     DAJSMI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.218"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            66..314
                     /locus_tag="ON430_002629"
     CDS             66..314
                     /locus_tag="ON430_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3220509.1"
                     /translation="MHWLWVLIVGAIIGLIAGAITNKGGSMGWIANIVAGLVGSAIGQ
                     ALLGSWGPQLAGMALIPSIIGAIIVVAVVSFFLGRSRG"
     gene            514..918
                     /locus_tag="ON430_002630"
     CDS             514..918
                     /locus_tag="ON430_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3220510.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            1066..2109
                     /locus_tag="ON430_002631"
     CDS             1066..2109
                     /locus_tag="ON430_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220511.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            2124..2687
                     /locus_tag="ON430_002632"
     CDS             2124..2687
                     /locus_tag="ON430_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR3220512.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLIDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
BASE COUNT          958 a          493 c          609 g          848 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat atttcacaga aagaaagaga
       61 aaattatgca ttggttatgg gtattaatcg taggagctat tatcggtttg attgccggag
      121 caattacaaa taaaggtgga tcaatgggat ggatcgctaa catcgttgca ggtttagttg
      181 gttcagcaat tggacaagca ttgttaggta gctggggtcc tcaattagca ggaatggctt
      241 tgattccttc tatcattggt gcgatcatcg ttgttgctgt agtatcattt ttcttgggac
      301 gttcaagagg ttaatcgtca aatatatcag caattttacc tattatctta aggagcagct
      361 tttcgctgct tctttttttg tatagataaa ctgcttctat cactgatcat cgatgttgtc
      421 taactgatgt cgccgaatgc aagcattacc tttgtcagat taaaaaatac tcgttacact
      481 taatatagaa cgacgaaaga aaggatgatc tcaatgaaaa aaatgtacga aacagcaatg
      541 atcaatcgtg gaggccgtga tggcgaggtt gaggcaccaa acggaagtat gcacatgaag
      601 atcgaccgcc caggaatcca tagtgaaggg acaaatccgg aacaactttt tgcggctgga
      661 tacgcatctt gcttcaacgg cgcggttcaa cacatgctgg aagaaaataa tcttgagtct
      721 gattctgaag tcaaagcacg ggtatcttta tttcaattag aagatggggg ctaccaaatc
      781 ggggttgttt tagaagtttc tttgccagga cttgagaagg accaagcaga gaaaatcgca
      841 gaagaagccc acgcattttg cccatactct aaagcaacaa gaggcaatat tgatgtggaa
      901 gtgactatag tagaataagg gattcttaat gttactataa atgagattat gaaagatcct
      961 ataaagaaac gctagtaaaa gaaacgctag taaaagaaac atgtagttac ggtaattaat
     1021 agcggtatat gtacgattga tcgaatagaa caaggaggca aaaaaatgac acagcgggta
     1081 gcaataatga ttttagtgtt gctggtcatt ggcctccttg tttactatgt cctaaagttt
     1141 aagcattgga agcagcaacg gatacatcag gatattgaaa aaaagttaaa acggtatcct
     1201 attgttcaag cagcttggga aaaggctgaa gcaaaacaat ataacattcc tggattaaca
     1261 gaaacaagaa tggtggtgcc ggaaaccggc gaaaatgagg tttgtcaatg gatgacgccg
     1321 caagggttgg ctttttctca agattttgta tttatttctg cctactgcta tgaccatcag
     1381 catcattcta ttattcatgt actggataga gaaacaggac agccgatcaa actgctgatt
     1441 ttacctaaac ggccacatgt agggggctta gtatatgata caaaacggga acttttatgg
     1501 ttgacaatca caggatctgc tactggacga gtagccgctt tacgtttgat cgatatctta
     1561 gcagatacat ctgaagagac gggacagcca atcgcttatt ggctgacaac tgatctctca
     1621 gaaattccac aagcatcgta tttgactcaa aacaatgatc aactggtatc tggcaacttc
     1681 acactaaaag gtgaaggaca gttgactttc tatctattgc cgacgatagc tgaaatgaaa
     1741 acagcaattc gccgcaaaga caaaatccaa ccaaccgtga caatgagcag aaaaacatct
     1801 gataagattc aaggtgtggc tttttatggc cattatctct tagccagtcg ttcttatggt
     1861 ccttatacct cggaactctt agtgtccgag cgggactctc ctagccggat cctaaaacga
     1921 atcaaatttc caccgtattt ggaacaaatc gttgttgtag aagatcgtct agcagtattg
     1981 tttgaaagtg gcgccgcagc gtatcgcgag aaagccaatc ctgtgttagc caatgtttta
     2041 ttactagact tagcaacgtt gcttcatgca aataagcgtc ccaaaaagat tgctgcaact
     2101 aaaaaatagt cggacaactc aagatgatca ctgttgttca taaagaacga caagaaaaat
     2161 catctagcga tgttcgacta tgggtttatt taggaagtat ttctactaag attcaatatg
     2221 gctatacaga ttcggcgaaa aaacaaggca atgttaagtt tttgagaatc acagatatac
     2281 aagagggacg tgtaaattgg tccagtgtgc cttactgtga tattagtaat tctaaactaa
     2341 ttgatttaag attagaagaa aatgacattt tgatagcaag gacaggagga actatgggaa
     2401 aaagtttctt ggttaaggaa ataagtgagg aatctgtttt tgcttcttac ttgattcgaa
     2461 ttaggttagt tgagaaatta ttatcagaat acgtagactg cttcttagac tcgccattat
     2521 attggaagtt attagaaaaa atttcttacg gaactgggca accaaatgta aatggtacaa
     2581 atttatctaa actacttata ccattaccac ctttagaaga acaacaacgc atgactacta
     2641 aaatagaaat gataagaaga tctattcgta gaatcaattt tgagtaaata taacggacct
     2701 agcatccatt ttataccaga tgatgctagg tctattattt atattttatg gaatttaatt
     2761 gatttgttct agtgttttaa tattattacg acagtaaaaa aatagtttga ttcttctaac
     2821 atgaatgacc atctagaagt ggaaatgtat aaaagattta ctttatgaag ttttcctaat
     2881 atcattcact catgatacgc agcgcctt
//
LOCUS       DAJSMI010000215         2793 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJSMI010000215 DAJSMI010000000
VERSION     DAJSMI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.219"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..184)
                     /locus_tag="ON430_002633"
     CDS             complement(<1..184)
                     /locus_tag="ON430_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3220513.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYV"
     gene            416..1150
                     /locus_tag="ON430_002634"
     CDS             416..1150
                     /locus_tag="ON430_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3220514.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            1189..2331
                     /locus_tag="ON430_002635"
     CDS             1189..2331
                     /locus_tag="ON430_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3220515.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         1058 a          443 c          473 g          819 t
ORIGIN      
        1 aaacatattt tctaatcgtt cctactgata cacctagata ttcacttgcc tgctgatagt
       61 ccatatactc tagagtgact ccagaaataa tgtcttctag cataggcttt agcactgtaa
      121 ttatctcttt cacttgttgt tcaacttgta tagaaacaat gctatcaatg atttgtagag
      181 acatatttcc ccctcctttc gtgattgctt gaacaaattt tctaagaatt atcttcgtat
      241 tttttgctca aaataaaagt attttgttat taaccgatta atcaaacaaa aaatgattac
      301 cagttcatcg acatacacat aatacctcga taaaaaaggg gtgtcaatag ttttgtaaaa
      361 tttatttaca acaccccttt tttgaggtat actaatttta ggaggtgtgg tcaatatgat
      421 aaaacgtcca ccaataaatt atttagaacg aaaaaagata ctcggaacaa aaataaaagc
      481 tataagaaag agtaaaaaat taacacaacc agcatttggt cttatgatta acaacggtca
      541 attaattgac aaaaagacta tctacgaatg ggagaaggga acctatttac caataccaga
      601 gcgattatcc cgaattgctg atttgggaaa tatgagtata gaggagttag tttgtggcaa
      661 tgtagaagaa tatattttgg gaattattct ttatagagat agcattgtac tagatggtat
      721 aacatttcca gataaaaacc tgttccagca tcttcgtcaa cagttcccac cagtacactc
      781 taatttagat acatggctgg atagatattc caaattagaa ccagagatgc aagaatttat
      841 tgctaacaag acttgtaata aagttaaaaa tgaaaaaatt agtcttttca atatcttaaa
      901 aatagaagaa ttattcatca atgctattgt agaagaattt gacaataaca ttcttttcct
      961 cactagcagt atagaagaac tactagaaag aatggtagat gaatggctac ctattcaatt
     1021 gaaagacatg tcttatcccg aagaagctgt gagagaaatt actgataata taaacaagct
     1081 agaacaaacg atttcatcaa ttgggaaaaa atacactaag aaaaaaatga agggaggtga
     1141 taccatctag tggtaagtac tccttgtaaa ctaagaggag atgatttcat ggcaaaaata
     1201 tcaaagtaca aaaagaaaaa cggtcaagtg gcgtggatgt ataaaggaca cattgccacc
     1261 gatccacgaa ctggtgaaaa aataaatact acgagaagag gttttagtag caaacaagaa
     1321 gctcaaaaag attatgacga gtacagacat caaatattat acggtgttaa aaagaaagca
     1381 cctgatatga catttgaaga cctgtataat gaatggataa ctcatcagcg taccagtgtt
     1441 aaagcttcaa caatcgcaat ctcagtaaga tacgcaaata atcaaatact tccagcattt
     1501 ggtaagctta aactatcgaa tatctctgtc ccatattgtc aaaaagtggt agatgaatgg
     1561 cacagtaaat atgaaagtta cgattatatg agaaaacaga ctgcacaaat tttacgctac
     1621 ggggttgcaa tgcaatatat tgacaataat cctatggaaa aaacacttct tccacgaaaa
     1681 aaagagtatg agaaaaatcg taaattttac tcaaaagagg agcttaataa tttactggat
     1741 gcttttaaag attttggtaa catgaaacaa tacgccttct tcagattatt ggcatacaca
     1801 ggtatgagaa agagtgaagt actcgcttta cagtggaaag acatagacac gtttaataaa
     1861 gaactacatg tcaataaaac tcttgctgta gatgaatttg gtaaagttat aattcaatct
     1921 ccgaaaacaa gagcttctag acgagttatc tctttagata ctgagactct ttctatcctc
     1981 aacaactgga aattacagca aaaagaagag tatttaaaac ttggatacaa tactagctct
     2041 aaagagcaac atgtttttac aacagttaaa aacactctat acattccaaa cactgttaat
     2101 gattggttaa gatatatttt gaaaaaatat aacttgcctc gtattacacc tcatgggttt
     2161 agacatacac atgctagttt attattagaa gcaggagaat ctgtcaaggt agttcaacaa
     2221 cgattagggc atgaaaatag taaagtgaca ttagacatct atgcacatat tacaaataat
     2281 gcacctaaga aaacaggtca agactttgct gatatgatgg ctcatcagta aaagagctgg
     2341 tcaaaatctg gtcatttcta gtatacaggc aaaacaaaaa agctagaacc cttatatatc
     2401 aagggttcta gctcctataa caggtttacc caacactgcc ttccaatgac tcttttggga
     2461 atcagaggaa agaatcaggc tttatagaga tatttcatct aacaaattac tttagactga
     2521 cagtatatat cagcaggtac tgtcatcggg aacgtagcca atatcgtatc caaaacagca
     2581 ggtagagaag ctatttgagg attcaagtta ttctatttca accatgaagt taagttcatt
     2641 aaaaataaag atagttggta tttcaataat ttttgtatag agttaatcac tttattgatg
     2701 ctaattcatc ttgattggta ttataatcgt attcaagttg cgtggcgtgc ttttgctaga
     2761 aaatatataa ctgttcttta taaagaaata taa
//
LOCUS       DAJSMI010000216         2749 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJSMI010000216 DAJSMI010000000
VERSION     DAJSMI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.220"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..566
                     /locus_tag="ON430_002636"
     CDS             <1..566
                     /locus_tag="ON430_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220516.1"
                     /translation="NLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVN
                     VTVESLETINTNSGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWA
                     QLWNMMYFNIGPKVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTST
                     PFDEDLSFVNKKKCSYAYVKAIKAVVR"
     gene            563..874
                     /locus_tag="ON430_002637"
     CDS             563..874
                     /locus_tag="ON430_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220517.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            864..1217
                     /locus_tag="ON430_002638"
     CDS             864..1217
                     /locus_tag="ON430_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR3220518.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            1207..1608
                     /locus_tag="ON430_002639"
     CDS             1207..1608
                     /locus_tag="ON430_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220519.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            1601..2005
                     /locus_tag="ON430_002640"
     CDS             1601..2005
                     /locus_tag="ON430_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220520.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            2017..2625
                     /locus_tag="ON430_002641"
     CDS             2017..2625
                     /locus_tag="ON430_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3220521.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
BASE COUNT         1014 a          402 c          550 g          783 t
ORIGIN      
        1 ttaatttgcg caaattgcct tctaaagatg atgtttggta ttgtagcgac tgctgtagtt
       61 atctaagtct tgattatatt agaagctgtg aaaacggaga aaatattcta aaagatatgg
      121 caaacattgt aaatgttaca gtagaatctt tggaaactat aaacactaat tcaggaggcg
      181 cacagtgggt tattaaccgt cctaaagcga attattggaa aaaggtttat ctggattcta
      241 atcggctata tcgctacctt agagggcaaa aaacaaatat acaaatctgg acagccgaga
      301 tgtgggcaca gctttggaac atgatgtatt tcaatattgg tcctaaagtt cacgaggaat
      361 tagacttttg ttttgctact gatccaatag aaaaagttaa agaagtaaaa atcttacaca
      421 atgctggagt aacaacaaac gatgaagatt tatttttcaa agggagatac gtgacttcta
      481 cgccttttga tgaagattta tcatttgtaa acaagaaaaa atgctcttac gcatatgtta
      541 aagcaattaa ggcggtggtt agatgacgcc tgaacaagtg actgaagaat tgctaatagc
      601 tgtgaaggat aatatttacg ttacctggaa cgaagaagat gagtcaatta aaaagatgat
      661 agctaaaaat gctgtttatc ttcaaagtaa agtgagtaca acactttctt tttctcctga
      721 aagcttagaa tacggattgc taatcgaaag atgtagatac gactggaatc gtgctttaga
      781 tgagtttgaa caaaatttcg ctagtgagtt attaggtttc attcaacatt atgcgctaca
      841 agaatatatt gcaggtgatg tgaatggcga ataatcgtag actcgaagaa acattcaacg
      901 atggttggtt aaagattttg acgcaaacta ccaaaagaaa tgaactagga aaaaagattg
      961 gtgtagaaga tacagaaatc acttctttaa aatttagaaa tctttccatg agagatagtg
     1021 atataacagc tatggatgcg atgggatcga aattaactaa gaaagtaaag actccatttc
     1081 atccaatcgc caagaaattt aataaagatc aatattttat cgtaatcgat agtatgcgtt
     1141 acaacgttat ctatgccgat tacgataatt tttatatcta tttttatctt gaaagtgtgg
     1201 gtgaatatgg tgattgataa ttctaaagaa aaagaacgtt taaataagca aatttctgct
     1261 atcaaaactt ccttagaaga gcattttggc ctcaaactct ttcaagactc cgtaggcgag
     1321 gatgagctac ctgatgattt taattacttc attctcgaaa caggagaaat agaaatgatc
     1381 actgagccaa aatatagcgt gggtcaaaat ctatatctaa ctttctattc agaaaataga
     1441 gaagatttaa caggagactc actagatatt atttcattga ttcaaaatcg ttcgattcgt
     1501 tttcagagaa tggatcccaa tcatttaaaa ctagagaacc aagatcgcta tatcgatcaa
     1561 ttggtattta cgtttagacg attattgaag agtgattgtc atggctaaaa atagttggga
     1621 gctaaaaata aatggacatg atgaacttct tgtgcggatg gaacgctatt caagcgagag
     1681 cgaacgactg attaacgaag cattgaaatc aaaaggttcg gctattgcag tggataggat
     1741 tacagaaaaa attcctgttt ctgaagcaga tttaagaaga gggcaccaac acgcaaaaaa
     1801 tagtcgtcca cttaagactc aatacattaa tttgggtttc atcattagac ctacaagaaa
     1861 atttgagtat ttaaaatatc ctgatttggg gataggtact tctaaaagaa atcagccgga
     1921 cgaatttatg agaagaggat taggtcttgc acttgatcca attacagaac ttctgattcg
     1981 tcaattcgat aaattaaata aataggggga acaacaatgg ctaaaacaaa aactgtagta
     2041 acaacgttcg ataacgtaag tatcaaacga attgctttta attttaagaa cgcaggaaat
     2101 gcaatcgcaa cgaattgtaa tggacaatta gatggcgaaa cagaaatgca aacggtggtt
     2161 aaaaaatgtg gagcgacaga agtaaaatca aaatctaaac caatcaatat gacggtaaca
     2221 attactgcac atgtaccgat ggaagtttat cgacgtttca atgggttgaa acaagatgga
     2281 cgtattaaac caggcattta ctcttacggt cctgattccg taggcgaaga tttctcactt
     2341 gctgcagaga tcgtggatga cttcgaagaa aatagaaagt tagttggtat gttagcatgc
     2401 acttcgaata caggattaac attctctatt gaaaatggtg cggatgaagt agctgcgtta
     2461 gaactagaaa caaaagttat gcaagatgaa tttggtaaat tttatcatga agcaattgtt
     2521 gcagaacttg aagaagactt aacagatcaa tggatgacga atctatctgc tgatgtgatt
     2581 aaaaaaactt cagttgtgac aactacggcc actcaatcac agtaaaaaaa acggaggtag
     2641 cgaaatgaac gaagattact caaaaattga actaaacgat ggaacaattt tgaatttaga
     2701 acctaaactg aatatcaaga aattattgat gatcaataga gattttaac
//
LOCUS       DAJSMI010000217         2744 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJSMI010000217 DAJSMI010000000
VERSION     DAJSMI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.221"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>2744
                     /locus_tag="ON430_002642"
     CDS             <1..>2744
                     /locus_tag="ON430_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR3220522.1"
                     /translation="ANDDGLKSTLKEISAELTKNRAELKLEQAQLQQTGSESDKLGSK
                     LSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELINQQTAQQRLSNEID
                     KTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQATAGQSASEAEKL
                     AKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAKLNDAEREFEELGQ
                     AAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVDSANSVGSSQSKIQ
                     ANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGDLNNQDLQNITEQA
                     MVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLDKTHELGDNMAEYS
                     QLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDGRFEQNMDMFSEST
                     RKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSALGEDNAMKVVQSLT
                     DVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTIIDALEPVIDFLGK
                     MADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMMLIIGGIIAVIAGVI
                     VAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKETWSGFVDWVQDIWQ
                     GVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEVWSIIWADIVGIVQ
                     IPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAW
                     NGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWNSIKNTITSLVQAA
                     ATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSW
                     "
BASE COUNT          945 a          408 c          624 g          767 t
ORIGIN      
        1 gctaacgatg acggattgaa atctacgtta aaagaaatca gtgctgaatt aactaaaaat
       61 agagcagaat taaaactaga acaagctcaa ttacaacaga ctggttctga atcagacaag
      121 ttaggaagta aattatcttc tttagaaaag cagtatgaat tacaaagtca aaaagttgaa
      181 gtaactagcc aacgtttagc caatgcgaaa aaatattatg gagaaaattc caccgaagtt
      241 cagaaacttg aaagagaact gattaatcaa caaacagcgc aacaacgttt gtcaaacgaa
      301 attgataaaa cgagtaatgc actagctcaa gcaaaaggcg aaatacagac gtacgagtct
      361 acaatgcaac agttggatag cgaacaaaaa aatgttcaag ctagtgcttc tctgattgaa
      421 tccgaataca aaaaatggca agcaactgct ggtcaatcag cttctgaagc cgagaaatta
      481 gcgaaagccc aagaatatgt ttctcaacaa tctgaaaatg cggagaaaac gatagatatc
      541 ctaagacgac agttagaagc tacacagtct gaatttggcg ctacatccac agaagcaatg
      601 cagatggaag cgaagcttaa tgatgctgaa cgtgaatttg aagagttagg acaagctgct
      661 aaaaatgtag atacaactaa cttggacgat atcggaagca aaatagatat gaataattta
      721 atggaagctt ctgacgtttt aagcgacatt ggcgataagc ttacagaatt agggaaacaa
      781 gcagtggact ctgctaacag tgtaggtagt tcccagagta agatacaagc taattttggt
      841 ttgtctaaac aagaggctga agaattaacg aatgtagcca gagacattta ttataaaggt
      901 tttggagaat cgttagatca gtccacagat gcattgattt tggtaaagcg taatttagga
      961 gatttaaata atcaagattt acaaaatatc acggaacaag ctatggtcct agaaaacacc
     1021 atgggcgctg atatggatga aacgttacgt ggtgtaaatg gcttaatggt caatttcggc
     1081 ttgagtgctc aagatgcaat ggatttaatg gtttcgggta ctcaaaacgg tttagataaa
     1141 acgcacgaat taggcgacaa tatggcagaa tatagccaat tatggagtca aatgggatat
     1201 tcagctgatg aaacgttcgg aatgcttcaa aatggtttag atgcgggtgc ttataacctt
     1261 gataaagtca atgacttagt taaggaaatg ggaatatcgt taacagatgg tcgatttgag
     1321 caaaacatgg atatgtttag tgaaagtact agaaaagctt ttgaagagtg gaaaaatggc
     1381 ggaggaacac aaaaagacgt tattaattcc atgattcaag attttagcaa tatggatggt
     1441 caatacgacc aattaaataa agcttcgaca atttggtctg cgcttggcga agataatgcg
     1501 atgaaagttg tccaatcttt aactgatgtt aaccatacat ttgatgatgt tagtggatct
     1561 gcacaaaaaa tgaatgaaga ttctactact ccgttgcaag agttgaacgg gaaaatagct
     1621 gaattaaagg attcattagc tcctataggc aacacaatca tagatgcact cgaaccagta
     1681 attgattttc taggaaagat ggctgatgcg tttaataatc ttccacaacc agtacaggat
     1741 ttcattgttg ctataggtgg tcttacagca gcatttggca tactgttacc ggcaatacta
     1801 gccgtgtcat atttatttgg tccgatgatg cttataattg gaggaattat agctgttata
     1861 gcaggcgtta ttgtggcaat taagaactgg ggtgcaatta ctgactggtt tagtggttta
     1921 tggaaaaaat ttactgattg gttgggtgat acttgggaaa gtataaaaga cggagcctca
     1981 tcagtttggg atggggttaa agaaacctgg tctggatttg tagattgggt tcaagatatt
     2041 tggcaaggag tttctgattg gtttggagag ctatggagcg gattagttga aggagcttcc
     2101 aacatctggc aaggagtcca agaaacttgg caaacattcg ttgattgggt ttcaaatatt
     2161 tggaacggag tcaaagaagt atggtcgatt atttgggcag acattgtagg aattgttcaa
     2221 ataccatgga cattaataac gtcattgatt caagctggta ttaatattat cgtgggtatt
     2281 tttgatgtag ctggacagtt attgggcgca gcttggcaag ctgtttggac acctatttct
     2341 gatttcctta aaaatacttg ggatactatg acacaatgga taagtatcgc ttggaatggg
     2401 attgtaacta cattccatac tatatttgat ccagtagtgg catggtggaa tggtatatgg
     2461 acagctatta gtactacggc ttcaaatatt tggaattcaa ttagtgcaac agcttctagt
     2521 atttggaaca gtatcaagaa tacaatcact agcttggtac aagcagctgc tacagtaatt
     2581 caaaatattt ggtcaactgt atctagttgg ttaggtggaa tttggaattc aatcagctct
     2641 acagcatcaa atatctggaa tagtgtgact agtagtataa gcaatgctat aaacgcagct
     2701 aaaagtgcca ttcaaagtgt ttggaatagt atatcttcgt ggat
//
LOCUS       DAJSMI010000218         2731 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJSMI010000218 DAJSMI010000000
VERSION     DAJSMI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.222"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..1559)
                     /locus_tag="ON430_002643"
     CDS             complement(<1..1559)
                     /locus_tag="ON430_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220523.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMR"
     gene            complement(1583..>2731)
                     /locus_tag="ON430_002644"
     CDS             complement(1583..>2731)
                     /locus_tag="ON430_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220524.1"
                     /translation="GGEISDLLNDIVDHQTDMITGQNGGYVLLDPKEAPSRILIMDTP
                     DKNTARNVLQINNAGIGFSKTGINGTYETAWTLDGGFNASFITAGEIVGITIRGTTLI
                     SDGADYRTSIANGKMTWYSKKVNKDIMELEARDYVSADAGIVSYTMKTGGGFMIRNPQ
                     GNLVFSTWDNGNNRPFLSFGAPNFRYSNASYVTSGDGSSLSINGSAGNSWEFKVAGRT
                     MKFTSDGMLTLPGCFFGSWEDGKLARFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLL
                     NAYETPEYYFADYGEAVTGDNGKVRVDIDLMFAETVNLSRYMTHVTPTELVLCAVTHE
                     DIDHFIIETSKPNVLVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF"
BASE COUNT          798 a          584 c          429 g          920 t
ORIGIN      
        1 tcacgcatac cagtaccacg atatttatac tcactaatat tcggggttcg agtattgcct
       61 ttttctagtt tgagccactc aatttgaaca gctccttttg tagctgacgg gtattggtat
      121 atttccagat aattagttac cccactatct atatgttgct tagttacagt aaaagttaac
      181 tcccatgtat tagctaaccc ctcagtaggc ttcatgtctc ctactttaac gcggccagca
      241 gcaatataca cggcaaacga ttgaactgtt agtttagttg ctttcattga tatggtatat
      301 gtttggtcag cgatatagtt ttctgtattg cgtctaccat aaaccatata ttcactagtt
      361 ttgtgtggaa agacaactgt agggtcagca atattctcgc ctaaaggaac tttacctaac
      421 cagtaagggc catctagtaa gttagggtag tgtgctttta acttaacgtc atagctgaca
      481 ctaaccgttc ctttatattc actattaagt gagaatattt caacataaca gtttgttatg
      541 ctagttacat tatcttttgt tataccagta aatgtatgag tgaccatttt atcgcgttct
      601 acatttgctg gtattaatgc attaatgggc agttctatac caccttgttc atttaaatat
      661 ctaagcctaa cccctatagt attggtctct ccagtccaat ctgaaccgac actcatagta
      721 acgcttaatg tgtatgtcgt agatggtcgt aaagtggtta taagatacct attattagct
      781 tccgctgtac acggaatacc taagccaagc gataatatgt cgccggaatt agctgttcta
      841 gttttagtat gagttataac ccttttaaca ctatctttaa ctgtatgtcc attacctaac
      901 aaaggtccaa cccaaggttc tgtaatatat ggcattaaat tcgggttctc cgaataatca
      961 tagtccccga agtcgatgct gttactgtac atcacttgta agttacctaa cttagaaatt
     1021 tcttctttca gagcatctaa cttgtcttgt agcgttttag cttgaccagt taaatcagta
     1081 atctgttgat ttaagctatc cactctacct ttgatttcag ccataaaagc atcaaaagtt
     1141 tcgttgtact ttcgaatcaa ctcttctaat tgcgaaacat attcatcggc ttggccttgc
     1201 gaaatgtcag acactcctag tgagaaaaaa atgatatctt gcgttgttaa aatttgattg
     1261 tcttttctat attctacgta gcagtgttta taatatcctg cttcactcat aaatgtgcca
     1321 tcaagagaaa acgtgacttc ttcactagtt acactagttg caacattatc tacgtaacgg
     1381 ttagatggtg ttgttccttt taaagtaaat gttccgccac tcgtatccat ctgcaagcca
     1441 tttagaaacg gtttaaccgt caccgtaatc cctttatcac cttgacgagc cataatagct
     1501 ttggtgtagt ttaattcttt gctgaaatct aaagccaaat tatataaact gctagccatt
     1561 tatatacctc cttgtcttcg ttttaaaaac gtttttggtc aagcactgtg ctatcatatg
     1621 ctgtatcctc ttttaatcta atatcttcat accctagacg gtgtgccact aaattccatc
     1681 taactaatac gtttggctta ctagtttcaa tgatgaaatg gtcaatatct tcatgagtaa
     1741 cagcacacaa aactagttct gtaggtgtca catgtgtcat ataccgactt agatttactg
     1801 tttcagcaaa catgaggtca atatcaacac gaactttacc attgtcacct gtaacggctt
     1861 ccccataatc agcgaaataa tattctggag tttcataagc gttcaatagt cgttgtccat
     1921 aatgttctgt tggtacagtt gagtttttag tacctctaac agtaaaatct ttatatactt
     1981 gtaccgttga ttgttcaaac ctagcaagtt tcccatcttc ccatgaacca aaaaaacaac
     2041 ctggtaacgt tagcatacca tcactagtaa atttcatagt cctaccagct accttaaatt
     2101 cccatgagtt acccgcacta ccattaatgc ttaaagaact accgtcgcca gaagttacat
     2161 aactagcatt gctatacctg aaattgggcg caccaaaaga tagaaacggt ctgttattac
     2221 cattatccca cgtactaaaa accaagttac cctgtggatt tctaatcatg aaaccaccac
     2281 cagttttcat ggtgtatgat acaataccgg catcagcact tacataatca cgtgcttcta
     2341 gctccataat atctttgtta actttttttg agtaccaagt cattttgcca ttagcaatac
     2401 ttgttctata atcagcacca tcactaatta atgtagtacc tctaatagta attcctacta
     2461 tttcaccagc cgtaataaac gaggcattga atccgccatc taacgtccat gccgtttcat
     2521 atgttccatt aatgccagtt ttagaaaaac caataccagc attgttgatt tgtaaaacat
     2581 tccttgcggt attcttatct ggtgtgtcca taatcaaaat acgactaggc gcttctttag
     2641 gatctaataa aacataacca ccattttgac cagtaatcat atcagtttga tgatctacaa
     2701 tatcattgag taaatcactg atttcgccac c
//
LOCUS       DAJSMI010000219         2708 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJSMI010000219 DAJSMI010000000
VERSION     DAJSMI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.223"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(737..2077)
                     /gene="glnA"
                     /locus_tag="ON430_002645"
     CDS             complement(737..2077)
                     /gene="glnA"
                     /locus_tag="ON430_002645"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCR3220525.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(2105..2497)
                     /locus_tag="ON430_002646"
     CDS             complement(2105..2497)
                     /locus_tag="ON430_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3220526.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          796 a          587 c          445 g          880 t
ORIGIN      
        1 ttttctttca cccttattta cctcctttac gtaaaaaacg tagtccacag ttctcggatt
       61 tttggcatat cttttctatt aaataatatc aaaaacaaaa caattccact aattttttca
      121 aagatgacct ttttttcaac aaaagtgcct tgtagggatt cattgaaggt aacgaatcac
      181 ataagtaaaa aataaggaga tgcgacaaaa gtcgtgcctc cttatttcta aataaacggt
      241 gttctcctgc caactcctcg gtcacaagtc acaatatcca tcaaacatga gaagctgttt
      301 ttgaaattat ttcttgtgat tcggagttaa atggtaggct cacaacctct ggataaacgg
      361 tattcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt
      421 ttcacaaaat ttttcttaat actcgaaact aaacgtcttt ttcaaaacct cttggtaaac
      481 ggtgttcaaa agcttgcatc tgcggtaata agttcagcca attcaaaaac aagaaaagct
      541 gtttttgctt ggctattctt attacttgtt gcagggcagc tttttcacaa cctctccact
      601 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
      661 ctatttttcc gttagtttct ctaatactca ggtcaaaacg ctgatttcac agcctctaat
      721 tcacggtatt cctgttttaa taaagttcta gatattgttc tctttcccac tcggaaacag
      781 tttgtctgaa tgccgcccat tcgagacgtt ttgcttcaac aaaatttgag aagatatgtt
      841 gtcctaatgc gtcaatcatt acattatcct tacgcaattc tttgatagca ttatggatag
      901 ttgaaggtaa gtcctggata tgtgcagctt ctctttcttc ctcatccatt acataaatgt
      961 tacggtcaac agctgctggc ggtgtgatct gattacggat accgtctagt cctgcttgca
     1021 acaatactgc cattgccaag tatgggtttg cagatggatc aactgaccgc aattccaaac
     1081 gtgtggataa accgcgagat tctggtacac gtactaatgg cgaacgatta cggccactcc
     1141 atgcgacata aacaggcgct tcataacctg gaacgagacg tttataagaa ttgactgttg
     1201 gattgcaaac ggctgtgtag gcacgagcat gtttcagcaa accacctaag aaatgataag
     1261 ctgtttcact tagttccaat tcaccgtctt tatcatagaa aacatttcct tcatcattga
     1321 acaaagacat attgcaatgc ataccagaac cattgatccc aaataacggt ttcggcatga
     1381 aagtcgcgtg aagtccatgc ttgcgtgcaa tcgttttgac aacgagcttg aaggtttgga
     1441 tattatcaca agcatctacc acatctgcat atttaaagtc aatttcatgc tgtcctggcg
     1501 ctacttcatg atgtgaagct tccacttcaa agcctagact ttctaattcc aacacgatgt
     1561 cacggcggca attttcacct aaatctgttg gtgcaaaatc aaaataacca cctttatcat
     1621 tcaaggtcgt tgtgatccca ccgtcttcgt ctaatttaaa taagaagaat tctggttcag
     1681 gtcctaaatt gaaagaggta aaaccaaggt cgcgcatatc tttcaatgct cgtttcaaat
     1741 tcccgcgagg atcgccggca aatggtgtcc catccggatt atagatatca caaattaaac
     1801 gagcaacttt tccatgagca ctttcccacg ggaagatcat ccaagtggag agatctggat
     1861 acaaatacat atcgctttct tcaattcgaa caaatccttc aatcgaagaa ccgtcaaaca
     1921 tcattttatt ttctaatact ttgtcgatct ggctcactgg aacttctaca tttttgatcg
     1981 tacctaagat gtctgtgaac atcaatcgta aaaatcgtac gttttcttcc tcaatgattc
     2041 gtttgatttc ttcacctgtt atctgtttct ttaccatggt atccctcttc cttttttaat
     2101 ttattcagtc aaagaattgt ctttgcaatc cttgcgactg ataatgacct gttttgctta
     2161 acccaccttg cgccaaaatc tcatcacgaa aaatttgtcg aacgtcatga tctgtcaacg
     2221 gcttcttgtt ttttgcttct tgttttttcg cctgtgccat ttcgtataca cgtttgatcc
     2281 cagccatatt caatccctct gaaagatagt ctttaatctc aagcaacata tcaatatcat
     2341 tcagcgaata catacgtcgg tttccttcgc ttcgttccgg atgaactaaa tcttgttctt
     2401 cataatagcg aatttgccga gcagacaaat cagtcagctt cataactgta ccaattggaa
     2461 atacggacat agatcggcgc aattcctttt ctcccatcag acgtcccctt tcgttttata
     2521 agttggctaa agtgtaccaa ttaaaaaaaa tattgtcaac tgcatatgtt aggttttcta
     2581 acatgagatc tctatttttt ataaaataac aaaaaaataa gaacacaaaa aaggtatcta
     2641 gttgattttc gtaccagata ccttttattt tttattatac tgtactatcc tttattagtg
     2701 tttttatt
//
LOCUS       DAJSMI010000220         2701 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJSMI010000220 DAJSMI010000000
VERSION     DAJSMI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.224"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            40..717
                     /locus_tag="ON430_002647"
     CDS             40..717
                     /locus_tag="ON430_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR3220527.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            707..1849
                     /locus_tag="ON430_002648"
     CDS             707..1849
                     /locus_tag="ON430_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR3220528.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1862..>2701
                     /locus_tag="ON430_002649"
     CDS             1862..>2701
                     /locus_tag="ON430_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3220529.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYS"
BASE COUNT          979 a          342 c          534 g          846 t
ORIGIN      
        1 aacattgaat caaatattga atgatgggga agatattaag tggattcaaa aacaaatagc
       61 ttattaaaat atactagtga tccatacatt attattgggg ctggcggaca cgcaaaagtc
      121 attattgaaa ccttaagatt agaaaataag ttaatatatg gattttttga tgataagcaa
      181 aataaaatgc aagatctgaa atatcttggg aaaataaacg aattgaatag ctgggcggat
      241 agcaaaaaaa aaatctataa atttattgta gctattggtg actgtgtagt aagaaaaaag
      301 attataaaaa aattgaatat gccaagtgag caatatggaa tagctgttca tccaagtgct
      361 atagtggcaa aagaaatttc aattggttat ggaactgtta tttttgcgaa tgctgtaatt
      421 aacccagatg ctgtaattgg agagcatgct ataataaaca caggtagcat tgttgagcat
      481 gattgtagaa ttaataacta tgttcattta tctccaggtg tatgcttagc gggtggagta
      541 catgtaggtg taggaactca aataggaatt ggttctcaat gtatccagat gaagaaaatt
      601 ggatcttggt gtatgatagg cgcagggagc actattgtta aggatattcc ctcacattcc
      661 ttagcttatg gcaatccagc taaaattaag aaagaaggaa ttaattttga attctagaat
      721 tttattatca agtcctcata tgggaggaac agaagaaaaa tatgttgcta aggcttttaa
      781 agataattgg atagcgccat taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa
      841 atatactggt tctaagggcg gagcttctgc gttaagttca ggtacagctg ccatacatct
      901 tgccttaaga ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt catttacttt
      961 tatagctaca gtaaatccta ttttatatca aggggctgta ccagtgttca ttgactcaga
     1021 acctaataca tggaatatga gccctgttgc actagagagc gcattagaag aagctgaaag
     1081 aaacaaccaa ttacctaagg caatcatcgt agtgaattta tatggtcaaa tggctgatta
     1141 tgaacgaatt ttagctattg caaaccaatt taatgttcca gtgatagaag atgcagcgga
     1201 atcgttaggt tctacttata agggaatttc gtcaggctta tttgggacaa ttgggatcta
     1261 ttcatttaac ggtaataaga tcatcacaac ctcaggtgga ggagctctag tgtctagtga
     1321 taaaaaatat gttgagcatt caagattttt agcaacacaa gcaagagatc aagcaccgta
     1381 ttatcaacat agcgaaatag gttataatta ccgtctaagc aatatttctg ccggtatcgg
     1441 acgtggacaa atggaagtat tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg
     1501 atataaggaa gctttttctg gaattgatag aattgaaatg atgccagaag tggatggcat
     1561 ggtgccaaat cgttggttgt ctactttaac gatcaatgat tataattcta ttgatcaagt
     1621 agttaacgta ataaataaac taaatgaaaa aaacattgaa acaagagctt tgtggaaacc
     1681 actacatttg caaccagtat ttcaaggggt aaaaatgtat taccatgata ataatcttag
     1741 tgtgtcagaa aagttattta atagcggctt atgcttacct tctggaagta atatgacaga
     1801 tagtgaacaa gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt
     1861 tatgaacata ggaattattg gtttaggttc catggggaaa agaagactta gactcattca
     1921 agaacacttt aatgatattt tactatgtgg tgttgatact aatgaggcga gacgaaagga
     1981 agttgaaaag gaatttggaa taccaactta taatagtcta acagaggcaa aaaatttgtt
     2041 tgaaataaat tcagtttttg tttgtacatc ccctataacc catgaattta ttataaaaga
     2101 ggctttaaat ttagattgta atatctttac ggaaatcaat ttattaaatg aatattatga
     2161 tgaaataatc gaagtagcta aaaagaaaga tagattactt tatctatctt ctacattttt
     2221 gaagagaaaa gaaattcaat atattaagtc taaagtgacg cataataaaa aaatttctta
     2281 tcaataccat gtaggacagt atttacccga ctggcatcct tgggaagatt atcgcaattt
     2341 ttttgtttca aacaaaaaaa ctaatgggtg tagagaaata ttcgctattg aattaccttg
     2401 gattatagat acatttggga aagttaaatc gtttactgta atgacccaga aattatccaa
     2461 tcttgatatt gattattcgg acagtttcac tcttattatt gagcatgaaa ctggtatagt
     2521 tggaacttta agtgtagatg ttgtttcaag agtagctaaa agaaatttaa ctataattgg
     2581 tgaaaagatt ttaatagaat gggaaggtaa cccggattct ctattttcat ggaatgaaga
     2641 aacttctagt atgacagata ttcaattgta cgatgaggtt atgcatgata ataattattc
     2701 a
//
LOCUS       DAJSMI010000221         2686 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJSMI010000221 DAJSMI010000000
VERSION     DAJSMI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.225"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..1514)
                     /locus_tag="ON430_002650"
     CDS             complement(<1..1514)
                     /locus_tag="ON430_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220530.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLAEPYNMCREYPNENIANPTVKFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRILQSPKETASACQIDWLKIEKGDTRTPNIEQYKYRGIGMR"
     gene            complement(1538..>2686)
                     /locus_tag="ON430_002651"
     CDS             complement(1538..>2686)
                     /locus_tag="ON430_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220531.1"
                     /translation="GGEISDLINDIVDHQTDMITGQNGGYVLLDPKEAPSRILIMDTP
                     DKNTARNVLQINNAGIGFSKTGINGTYDTAWTLDGGFNASFITAGEIVGITIRGTTLI
                     SDGTDYRTSIANGKMTWYSKKVNKDIMELEARDYVSADAGIVSYTMKTGGGFMIRNPQ
                     GNLVFSTWDNGNNRPFLSFGAPNFRYSNASYINDGDGNSLGIDGSAGNSWEFKVAGRT
                     MKFTSDGMLTLPGCFFGSWEDGKLARFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLL
                     NAYETPEYYFADYGEAVTGDDGKVRVDIDPMFAETVNLSRYMTHVTPTELVLCAVTHE
                     DVDHFIIETSKPNVLVRWNLVAHRLGYEDVRLKEDTAYDSTVLDQKRF"
BASE COUNT          763 a          574 c          434 g          915 t
ORIGIN      
        1 tctcgcatgc cgattcctcg gtatttatat tgctcaatat tcggggttcg tgtgtcgcct
       61 ttttcaatct tgagccagtc aatttggcat gcgctggccg tttctttagg cgactgcaaa
      121 atgcgaaggt ctttaggcaa acccggttca agtttcgttg gtgtgaatgt tagagaccat
      181 acgtctgtca atccttcaac tggttttagg tctccaaaat taacgttcca ataattatat
      241 gctacaaagg tttgacttgc gggttttgtt cctttaagcg tgatagtata cgtttgacct
      301 attataagct cttcttccgt gttaccttta tatatttcgt acgcgctaga tttaattggg
      361 aacttaactg tagggttggc aatattctcg ttaggatatt cgcgacacat gttgtaaggc
      421 tctgctaata agtttggctg atatggggtg gctgttgagc cttcttcgat tttgatgtcg
      481 tacaacttaa atccgccatt tattttatcc ctgtcaacaa agctaatact catataaaat
      541 cgatctaaat ttgtgatttg ataattaacg ttagctgtac ctttgattgt tatttctttc
      601 cctacatcat ctgttgtaat atttgtacta tttgcttcca ataatatctt ttctcctggt
      661 gatgtacgat acaccaaacg taatttatca atagctcctg tagttccttc atcaaatcga
      721 actttcgcac tcagagtata ggttttccca ctagcaagct gaggtgtatt aatacgcgta
      781 aacataataa tgcttcctgt accatcagaa gtaaaatgta acttttcgtt atccaaagtt
      841 agtgacccgt ggtaaccaac gttaaaatcg ctcgatttta gtttggacat taaattcgga
      901 ttcccactat aatcatagcc cccgaagtcg atgctgttgg agtacatcac atgtaagtta
      961 cctaatttag aaatttcttc tttcagagca tctaacttgt cttgtagtgt tttagcttga
     1021 ccagttaaat cagtaatctg tttatttaag ctatccattc tacctttaat ttcagccaga
     1081 aaagcatcaa aagtttcgtt atactttcga atcaattctt ctaattgcga aacatattca
     1141 tcggcttggc cttgcgaaat gtcagacact cctagtgaga aaaaaatgat atcttgcgtc
     1201 gttaaaattt gatcgtcttt tctatattct acatagcagt gtttataata ccctgcttca
     1261 ctcataaatg tgccatcaag agaaaatgtg acttcttcac tagttacact agtagcgaca
     1321 ctatctacgt aacggttaga tggcgtagtt ccttttaaag taaatgttcc gccgctcgta
     1381 tccatctgca agccatttaa aaacggttta acagtcaccg tgatcccttt atcaccttga
     1441 cgagccataa tagctttggt gtagtttaat tctttgctga aatctaaagc caaattatat
     1501 aaactgctag ccatttatat acctccttgt cttcgtttta aaaacgtttt tggtcaagca
     1561 ctgtgctatc atatgctgta tcctctttta atctaacatc ttcataccct agtcgatgtg
     1621 ccactaaatt ccatctaacc aatacgtttg gcttactagt ttcaatgatg aaatggtcaa
     1681 catcttcatg agtaacagca cacaaaacta gttctgtagg tgtcacatgt gtcatatacc
     1741 gacttagatt tactgtctca gcaaacatgg ggtcaatatc aacacgaact ttaccatcat
     1801 cacctgtaac ggcttcccca taatcagcga aataatattc tggagtttca taagcgttca
     1861 atagtcgttg tccataatgt tctgttggta cggttgagtt tttagtacct ctaacagtaa
     1921 aatctttata tacttgtacc gttgattgtt caaatctagc aagtttccca tcttcccatg
     1981 aaccaaaaaa acaacctggc aacgttagca taccgtcact agtaaatttc atagtcctac
     2041 cagctacttt aaattcccat gaattacctg cactaccatc aatacctaaa gaattaccgt
     2101 caccatcatt tatataacta gcattactgt atctgaaatt tggcgcacca aaagataaaa
     2161 acggtctgtt attaccatta tcccacgtac taaaaaccaa gttaccctgt ggatttctaa
     2221 tcatgaaacc accaccagtt ttcatggtgt atgatacaat gccggcatca gcacttacat
     2281 aatcacgtgc ttctagctcc ataatatctt tgttaacttt ttttgagtac caagtcattt
     2341 tgccattagc tatacttgtt ctataatcag tgccatcact aattaatgta gtacctctaa
     2401 tagtaatccc tactatctca ccagctgtaa taaacgaggc attgaatccg ccatctaacg
     2461 tccatgcggt gtcatacgtt ccattaatgc cagttttaga gaaaccaata ccagcgttgt
     2521 tgatttgcaa aacgttccgt gcagtattct tatctggtgt gtccataatc aaaatacgac
     2581 taggggcttc tttaggatct aataaaacgt aaccaccatt ttgaccagta atcatatcag
     2641 tttgatgatc tacaatatca ttgattaaat cactgatttc gccacc
//
LOCUS       DAJSMI010000222         2506 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJSMI010000222 DAJSMI010000000
VERSION     DAJSMI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.226"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            1..948
                     /locus_tag="ON430_002652"
                     /pseudo
     CDS             1..948
                     /locus_tag="ON430_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1052..2224
                     /locus_tag="ON430_002653"
     CDS             1052..2224
                     /locus_tag="ON430_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HCR3220532.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSTIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          981 a          367 c          424 g          734 t
ORIGIN      
        1 agtgagctca ctggtgatat ctttaattat gagatcaaaa gatacagtat aagtggcatt
       61 gttataacaa ttcaaaatgt aggagagaaa ttaaaaatta gctttgagcc aactgattca
      121 tttcagtatc ttagcaaaga tattgaaatt gaagatattt ataaaatttt agctcaagag
      181 ctatctaaaa atgttattag tttgccaaag gttttttatt ttccagccga aagaactggt
      241 ataaatgtct ttaagaacga actaaatgaa agtcgcttaa aaacatatga taccttgctg
      301 aatactgtgc aatttactaa tttaactaac caaaaaaaga aagctgccct caagtacgat
      361 ctgttaaaaa agaatattga gctgttaatg gagacaacgg ataatagttc ttatccaaag
      421 ccaatatcgg attatattaa ttatctgaat aacatgaaaa atagatatga acagaaaaat
      481 gtcaataata tagcatcata tattagagat gagattatta tgggtaaata tgaaattgac
      541 gaaaaagata attctgtaag ttttagacaa aagtttggta aaaatagata tagaaaagaa
      601 cgaattcctt ttcacgttgt gtcttcttcg attaaatctc tatatggatt agattattac
      661 ttagaaaatt caagtaaaga aggagactat cttattattg atgagccaga attgagttta
      721 catccagaaa atcaggttaa gttagcaaaa gtaattacag atatagttga gagtaaaatt
      781 aagctaatta cttctaccca tagtgattta tttgtacgtg agcttacaaa tattgttatt
      841 gaaaataaat taaatagtaa gaataataaa aaaaataaat tgtctgataa aaatataggc
      901 atatataatt ttgtaggaaa taatgatata aatgaattag aaaaaatttc gataaagtcc
      961 gtataattgt gtaaaagtaa aaaggccata taacagtcct tttacggtac aatgttttta
     1021 acgacaaaaa catacccagg aggactttta catgacccaa gtacatttta cactgaaaag
     1081 cgaagagatt caaagcatta ttgaatattc tgtaaaggat gacgtttcta aaaatatttt
     1141 aacaacggta tttaatcaac taatggaaaa tcaacgaaca gaatatattc aagcaaaaga
     1201 atatgaacga acagaaaacc gacaaagtca acgaaatggc tattatgagc gcagctttac
     1261 gacacgtgta ggcacgctag aattaaaagt acccagaaca cgtgatggcc atttttcacc
     1321 cacagtgttt gaacgttatc aacgaaacga aaaagccctc atggcttcaa tgttggaaat
     1381 gtatgtatca ggcgtttcaa ctcgtaaagt atcaacaatt gtggaagaac tttgtggtaa
     1441 atccgtctct aagtccttcg tttctagctt aacagaacag ctagaaccta tggttaacga
     1501 gtggcagaat cgtttattat cagaaaaaaa ttatccttac ttaatgaccg atgtactcta
     1561 tataaaagta cgagaagaaa atcgagtact ctcaaaaagc tgtcatatag cgattggaat
     1621 aaccaaagat ggcgaccgtg aaattatcgg cttcatgatt caaagtggcg aaagcgaaga
     1681 gacctggaca acattttttg aatacctaaa agaacgcggt ttacaaggta cggaactcgt
     1741 tatttctgat gcgcacaaag gattagtctc tgccattaga aaatccttca ccaacgtaag
     1801 ttggcaaaga tgccaagttc acttcctaag aaatatcttt accaccattc ctaaaaaaaa
     1861 ttcaaaatct ttcagagaag ctgttaaagg aatttttaag ttcacagata ttaacttagc
     1921 gcgtgaggct aaaaatcgat tgattcatga ttatatcgat caaccaaaat attcaaaagc
     1981 ttgcgcatca ttggatgatg gattcgaaga cgcctttcaa tataccgtac aaggaaattc
     2041 ccacaatcga ctaaagagta ccaatctaat tgaacgactg aatcaagaag tacgcagaag
     2101 agaaaagatt attcgcatct tccccaatca aacatcagcc aatcgcttaa ttggagccgt
     2161 tcttatggac ctacatgatg aatggattta ttcttcaaga aaatacatca attttgataa
     2221 gtagaaatgg taaaaacatt gtatagcatt ttacacagga gtctggactt gactaaaatt
     2281 tctaacatca gttattatga taattttgat gatgtcaatt atgatttaca ggaaagatat
     2341 aatgatttaa tcgaagaatt agaggagaga ggagaatgat ttcagataat aaatgggaaa
     2401 taaatctcaa aagatttatt tcagaatata tatatacgat tgaaactaaa tctagttttt
     2461 ctcttaatga agacagtgat cctctaaaaa aatacactat tcaatg
//
LOCUS       DAJSMI010000223         2501 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJSMI010000223 DAJSMI010000000
VERSION     DAJSMI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.227"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            175..>2501
                     /gene="esp"
                     /locus_tag="ON430_002654"
     CDS             175..>2501
                     /gene="esp"
                     /locus_tag="ON430_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCR3220533.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          909 a          386 c          503 g          703 t
ORIGIN      
        1 aatatccgaa aaaaacatga agaaatgtac tttttttgtt gtaatgttca tgaaattctc
       61 taagtaaatt agttaacata aggttagtaa gattcctatt ctgtgaataa tacgtatcag
      121 atttatcttt tattcttact aggaaagtaa ttattaaggg agttgatttg aataatggtt
      181 agcaagaata ataagagagt atttctagaa aaaacaaaga aacgagtatt gaagtacagt
      241 atcaaaaaat taagtgttgg ggttgcatct gttttgattg gtgttggtct tgtcttggga
      301 acaactgaac tagttaaagc acaagatgaa atttctccaa gcagcccact tgaaacagcg
      361 ataagctccg tacaagtagg tgacaaagta gcttcgggga atacttttca ggagaatcct
      421 ggttatacga aaaattataa cttttcggat ttacaattta gtcctcaaga actaactgga
      481 gacacactaa aaggcaatac aattggattt gaagtttacg gaaaacataa tattgcagca
      541 tcaactaaaa actgggaaat ccgtcttcaa ttagatgaac gattagctaa atatgtagaa
      601 aaaattcagg tcgatccaaa gaaaggaatt ggatcttcta gacggacctt tgtaagaatt
      661 aatgattcgc tgggtagacc tacaaatatt tggaaggtca attatattcg tgcaagtgat
      721 ggattatttg ctggggcaga aacaaccgat acgcaaaccg ctcctaatgg ggtaattacg
      781 tttgaaaaga gcctagatga aatttttaaa gagataggca tagacaatct caagaccgat
      841 cgtttaatgt atcgaatcta cttggtaagt catcaagacg atgataaaat tgtaccgggt
      901 attgatagta cgggctactt tttaacagat tcagatgatt tctataatag cttagatgta
      961 tctgaaaata atcctgacca gtttaaacat ggatcagtaa gtgcaaaata tgagaaacct
     1021 aatactcaaa caaaagatgg ttcaggatcc acaggtgcta atggagctat aatcttagat
     1081 cataagttaa cgaaaaatta taatttctca tattcatctt ccgcaaaggg cacgccttgg
     1141 tatgctaatt acaaaattga tgaacgatta gttccatatg tagcgggtat acagatgcac
     1201 atggttcagg ctgacaaagt aacatatgat gtttcgtttg aatccggaac aaaagtagct
     1261 gatttagcaa tcgagcgacg ggaaggtcat gaaaattatg gcataggttc aataactaat
     1321 aatgatttaa ctaaacttat cgattttgct aatgcaagtc cacgtccagt cgttattcga
     1381 tatgttttac aattgactaa gccattagat gaaatcttag aagatatgaa agcaacagca
     1441 caagttgaag aaaataaacc atttggtgaa gatttcatct ttgattcttg gttgtcggat
     1501 acgaataaaa aattaatcca gaacacttat ggaacaggtt attattattt gcaagatatt
     1561 gatggtgatg gaaaccctga cgataaagaa gagagcggag acacgaatcc atatatcggg
     1621 aaacctgaat tagaagaagt atatgatgtt gacacaacag ttaaggggaa agtattcatc
     1681 cacgagttag cgggaacagg tcacaaagcc caacttgttg ataaagaagg tactgtatta
     1741 gcagaaaaaa ctatcgctcc aaatgaaaaa gatggggctc caatttcaga tactgtagaa
     1801 tttgaattta cgggtgtaga ttcaagtaaa ttaatcgcga aagatgaatt aaaaatccaa
     1861 atcgtttctc caggttttga taaaccagaa gaaggttcaa ccgttattaa ggaatcacca
     1921 aaagcggttg ataaacaaac cgtggtagtt ggatttaaac cagatgctaa agaatcaatt
     1981 cggaataata aaaacttacc tgaagatgca gagtattcat ggaaaacaga gcctgatact
     2041 tctaacgtta ctgatagtac gaaaggtatt gtaactgtta agatcggaaa tcgaactttc
     2101 gacgtggatg tagagtttgc tgtaaaagct tctcaagcta tggaaaatga tgcaacatac
     2161 gtacctataa caacaactcc agaaacgaca gttcaaagtg gaaaacctac atttgataaa
     2221 ccagatgttc ctttagctaa agatgccttt tcaatttcag atgtctataa taaggacttc
     2281 ggtaatgcaa gtgttgatgc aaacactggt attgttacat tcactccagc taaaggtgta
     2341 ggagaatcgg agccgattac tggaacaatt cctattaaaa ttgtttacca agatggttct
     2401 gtaggcacga ccgatttagc agtaactgta agtaaagata tttatgaaaa tccaggagaa
     2461 aacattcctg caggctacca caaagtaacc ttcaccgcag g
//
LOCUS       DAJSMI010000224         2496 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJSMI010000224 DAJSMI010000000
VERSION     DAJSMI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.228"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(525..1028)
                     /locus_tag="ON430_002655"
     CDS             complement(525..1028)
                     /locus_tag="ON430_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3220534.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1387..2412
                     /locus_tag="ON430_002656"
     CDS             1387..2412
                     /locus_tag="ON430_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3220535.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT          797 a          394 c          464 g          841 t
ORIGIN      
        1 aataacatat ctcattttac acttttgtta ttttatactt aaaaaataac gacacagaaa
       61 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      121 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      181 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      241 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      301 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      361 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      421 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      481 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      541 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      601 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      661 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      721 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      781 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      841 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
      901 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
      961 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1021 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1081 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1141 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1201 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1261 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1321 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1381 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1441 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1501 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1561 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1621 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1681 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1741 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1801 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1861 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     1921 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     1981 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2041 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2101 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2161 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2221 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2281 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2341 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2401 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2461 aacctctaat tcaaggtgtt caaaagccag attctc
//
LOCUS       DAJSMI010000225         2480 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJSMI010000225 DAJSMI010000000
VERSION     DAJSMI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.229"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            366..1199
                     /gene="msrA"
                     /locus_tag="ON430_002657"
     CDS             366..1199
                     /gene="msrA"
                     /locus_tag="ON430_002657"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3220536.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1312..2268
                     /locus_tag="ON430_002658"
     CDS             1312..2268
                     /locus_tag="ON430_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3220537.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT          896 a          395 c          486 g          703 t
ORIGIN      
        1 atgtagaaat aaatcatcaa cctgtaaagg agcaggtgaa tgctaatgga ttggtacaaa
       61 taagcggagt caaaggagtt gctgatatta aagtaagcac aagttataca gtaacgacta
      121 ttatttctta tcttgttaca ttaactgcat atttatgttt attttttaaa atagttttcc
      181 ataaaaggta aattaacttt tcatttataa aataaattga ttaaaaagga gaatctgtcg
      241 ataaggattc tctctttttt atacgaaaaa tctgtagggg gatgaagtaa tcgcttattt
      301 cctcacttta tgataaatta cagctaaagt cactagcttg tcttcttcgg aaaggaggag
      361 aatctatgga tgaggaacaa atattcattc aaacacttta taatctgatt ttgaatccga
      421 atacacgaga ttgggaacga aaagtattga ttcagacaaa gaatgacata agagaaaata
      481 tatccgttaa agaacagtta tcaaaactag aagcaacact acgtccatta gctatccgaa
      541 tgaatctgac gcctgatgta atggactttt atttattgat tacggaagga tttgataagg
      601 agcaaaaata tgacttttcc aagcatgcca tgcaagatgc tgactatcaa gaacgtgcag
      661 tatttgcagg gggatgtttc tggtgtatgg tcgagccgtt tgaatcgaaa aaagggatcc
      721 tatctgtttt gtcaggatac acgggcggac atgttgaaaa accaaattat gatcaagtaa
      781 gtggtggtta cacaggtcat gtggaagctg tggagatcat ttatgacaca agagaaataa
      841 gttatagcga gttgctgaca atctattggc aaattactga tcccacagat acatttggac
      901 aatttcaaga tagagggaag caatatcgtc cagtgatttt ttatcaaggc gaacgacaaa
      961 aagaattagc ggaacaatca aaacagaaac tagacagttc aggaactttc caccaaccaa
     1021 tcgtcacaaa aatcgaacca gccggtacgt tttggccagc agaaaactat catcagcaat
     1081 tttacaaaaa acaaccgaaa cgttataaaa aaatccagca agcacgtaat caatttctga
     1141 tctatcaacg agtaaaaaac aaatggcaaa aaaatatacg aaaaaatcat tttgattaac
     1201 tccttttctt taaaatgtag taaactaaaa atagaaaaaa gaaagcgttt taaaaagatt
     1261 tgatgaggag gagaaaaatt gtatattgca gatagtgcac gttatgaaaa aatggaatat
     1321 cgtaaatccg gtcattcagg tttgaaatta ccgattttat ctctcggatt atggcaaaat
     1381 tttggtgatt atgatcctat ccataatcaa agagagattt taagaggcgc attcgacatg
     1441 ggaattaccc atttcgacct tgcgaataat tatggaggcc ctgctggagc tgcagagaaa
     1501 aactttgggc gtatttttag agaagatttt caagcctacc gtgatgaact gattatttct
     1561 agcaaagcag gttatcatat gtgggaaggg ccatatgggg aatggggctc tcgtaaatcc
     1621 atcatttcaa gttgtgacca aagtctccaa agaatggggc tagattatgt tgacatcttc
     1681 tatcatcatc gcccagaccc tgatacacct ttggaagaaa cagcagaagc tttgatgcaa
     1741 cttgtaagac aaggaaaagc attatatatt ggtatatcca actataatgg agaagataca
     1801 aaaaaaatga ctgaaatttt aaaaagaaag gaagcaccgt tcattattca tcagatgcgc
     1861 tacaatatgt tttctcgtgc actactagaa gacgatctat ctccagtatt agaagaagaa
     1921 gggctagggg caatcacgtt cagtccgctt gctcaagggt tgctgacaaa tcgttatcta
     1981 catggtattc ctgaagattc tagagcacat cgtaaggaaa taccattcct ttcagaagaa
     2041 caagttggat caactctgga aaaaatcaaa gcactgcaaa caatcgccgt atcacgagga
     2101 caatcattag cgcaaatggc ccttgcatgg aatttgcgac aaaaatctgt tacaagtgta
     2161 ctggtcggag caagtcggtt aagtcagtta caagaaagtg ttcgaatgat ggataattta
     2221 gatttctcac cagaagaaga attgagaatc gaccaaattc tagaatagaa acgatgtaat
     2281 caaacttttt atccatatat taaaaaaaac aaaagatgac atcaagaatc gaatgaagat
     2341 cgtctgttat tgaacatcat attttttgtc gttcaaaata aaaatgatcg aaggctagga
     2401 gtgaatttta tcctagtctt ttttatggta aatatcttct tcgtttgtat gtctatgaac
     2461 ggattaatca attttgtata
//
LOCUS       DAJSMI010000226         2452 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJSMI010000226 DAJSMI010000000
VERSION     DAJSMI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.230"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            22..504
                     /locus_tag="ON430_002659"
     CDS             22..504
                     /locus_tag="ON430_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCR3220538.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSEHMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLLNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            541..1551
                     /locus_tag="ON430_002660"
     CDS             541..1551
                     /locus_tag="ON430_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCR3220539.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1666..2379)
                     /locus_tag="ON430_002661"
     CDS             complement(1666..2379)
                     /locus_tag="ON430_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220540.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          833 a          402 c          500 g          717 t
ORIGIN      
        1 aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca cgaaaatata
       61 tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt ggtaataaag
      121 aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga taaaaaagat
      181 aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc attggggaag
      241 atcgtcggta aaaggctttc tattgtttca tcagaacaca tggaagaaca aacgggacaa
      301 aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat tattgatgaa
      361 gagctcttat taaatgaaaa acttgttttt gcaccaggta gaccagatca aacgatggtc
      421 atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat atttttgatg
      481 ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag gtgaagcaac
      541 atgataagtt atccattatt agaaagaaat acaacgattg gggttactgc accatcctca
      601 ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat ggaagaaaaa
      661 tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa atcaggtcca
      721 gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat ccaaatgatc
      781 tttccgcctt ggggaggaga attattgatt gagattctcg attttgtcga ttttgatgct
      841 ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact tgctgtcaca
      901 ttaaaaacag ggatagccac tgcacatgga atcaacctta ttgatttaag aggagagtac
      961 ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg agaatcgatt
     1021 ctacagcaac cttctgaaaa gtatcaaaaa gagtggcccc atgagcattc atcatcttct
     1081 gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc agtaaggatg
     1141 aagggacgat tattaggtgg ctgtattgat acgatcagtc atttaatcgg taccccttat
     1201 ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt gtggtttcta
     1261 gaaaattgtg agctatcgtc agttgattta agaagatcac ttgttcagat gaaactggca
     1321 ggatggtttg atggcagctc ggggatcatg ttcggtagaa gtgcagcaga ggatttgtta
     1381 gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact accgatcttg
     1441 tatgatattg attgtggtca tgtaccgcca caactgacat tgatcaatgg tgcctatgca
     1501 gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta aaaaattttt
     1561 tcctgccttg gagtttatca aagaccaaca aatctaaata gaaaagaaaa tgaaagaagc
     1621 tgacagtatt ttgccagctt ctttttgtat gatctattag aaattttaat tatccataac
     1681 tatcacattg atcagctgtt tcacatcatt tggattacct gtaggaatag ctactacaga
     1741 tgcattatca ataatttctt gagttgtttc tctaatgaac gctggataag atgtttgatc
     1801 tgcaggcatt tgccaatgtg taggaactgg ataactagtg attgtcccgt catggttact
     1861 tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca catctttcgg
     1921 atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat aatgaataac
     1981 atgtagttcg aaatcactcg atgtatctct aaaagtatta cctctaatag ctagccaaac
     2041 acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg actgagaaga
     2101 ctgtgaggaa gttgtgtcta ttgttgaaga agaaatattt gtctcaactg tactactaga
     2161 tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat tcgtttcagt
     2221 agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga ttgaactttt
     2281 gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc ctacaaggaa
     2341 taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct cattattttt
     2401 ttaataccag tatagcgtaa tcctgaattt ttgaatccgt aaaaccttgt ct
//
LOCUS       DAJSMI010000227         2422 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJSMI010000227 DAJSMI010000000
VERSION     DAJSMI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.231"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            79..1164
                     /locus_tag="ON430_002662"
     CDS             79..1164
                     /locus_tag="ON430_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3220541.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            2002..2334
                     /locus_tag="ON430_002663"
     CDS             2002..2334
                     /locus_tag="ON430_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR3220542.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
BASE COUNT          920 a          360 c          411 g          731 t
ORIGIN      
        1 tcacccttaa tacatggtat agttcacttg ttgaaaatcc tgaatatata caagtttcta
       61 agaagaagga gaaatttaat gtctacagaa aatcaatctc aaaaaatgat aattaaaaat
      121 tggggatata aagcaacgaa tgtttatatt gaaaaatatt ataaatttcc tagtttttta
      181 attcagaatt ctaaatactc taaaatgaaa gacagctcaa aaatagccta tatgcttttt
      241 aaaaatgaat ttcgtagagc cttacatgaa aaatggatcg acgatgatgg tctattatat
      301 ttggaattta cacaagcaga cctaatggac aaattaaatt gctaccaagg caaagtaaaa
      361 accattttaa acgagttaga aacatataat ttgatttcag tcgtaaaagg agaatttaac
      421 ccaaaaacga aaaaaaatga aaaaaataaa tactatttac ttcaacctga aataagtgcc
      481 gatgatcttt ttattcaaaa ttctttcgat gacgatttaa gcgaaactat tgataaatca
      541 acctcttctc ggaatgcgga aatcgcacgt cgagaggaaa ctgctaaaac ccttgataac
      601 actggaaatg cggaaatcgc acgtcgagaa aaaacagctg aaacccttga taacactgga
      661 aatgcggaaa tcgcacaaga ttattatatt aataaatctt tagacactaa aagacactta
      721 aaagacactg aaacagacca attacaaaat caagttttgc tagataactt cgttgaaatc
      781 atgaaagaag acagcataaa cacatttgta ccagaaaagg tattaagctt gataaaaaca
      841 ttttctaaca cttacgaaga agcccaacaa accgtcacca caatacacaa cgcaaaacat
      901 aaagccgagg aacttgagaa gacggtagtt gtgtatgaag aacttgaatc ttacggaatt
      961 gatgcggata aaggattata tatgacgttg ctgaaagctt atcaaaaaca aaagactgag
     1021 aaagtaaata atttgcagaa tttgattttt gtgtatgtaa aaaactggtt tattgaaaaa
     1081 gctattgcgg caaagcttgc aaacgaacaa agagaaacat ttaacgaatt accaacagtt
     1141 agtacggaaa attggctaga gtagttgata aaaagcgatc actataaaaa tgatcaattt
     1201 taaattagaa cttttttatg attttaattt tattacaaat gttacaaata aatagctatt
     1261 attccggagt ttttagatta aaagtttatg aaaacaatca caaacgtagg ttttaatgtc
     1321 ataatcgtta gatattttag ttatttttca ttcaaaaaaa cagggaacta gtgtttgcat
     1381 atgtattttt ttgagttata ataattcatg aagagaggta attcacttat ttacccaagc
     1441 tgaaaagcct gatattactt cttttcaggc agtgctgcta atattcaatt acactttttt
     1501 gtataaattt agttgtttta aatttaattg tctgctatgc ttgtgatgaa ttgattttcg
     1561 ttctgcactt ttggacgata aatttggaca attgaaaata caaaaaaaaa agaaaggccc
     1621 tgtgagactc ggaaggctac caaccttcgt cgacgtccac aggtggcatt aagatcactc
     1681 ccttaatgcg tttacctttc agtgttacgg tattacccgt aacggattta gtataacaga
     1741 tattcaaaag tgataccact ttttttggct gtgttaaggt taaaaatacg tgatattacg
     1801 atgaatctat gcctaagcat aatgttataa aaatgtccgt gtggctcttt gccgtatgac
     1861 gaacgctgaa attagaggga acgcatgttt ggatgcgttc cctctttttt gtttgtcatg
     1921 aacgaaaatc aattctctat ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa
     1981 agtaagtggg tggattattt tatgtcatat acaggaattt tgagtctcga agatatttgt
     2041 cactacggta aacggtgtac cgctacagaa aagatcacga agaaactatc aactggtcaa
     2101 agtaaaaccg ttgtgcagtg taagaaatat ataatccaga aagacaaagt ctcagaagaa
     2161 atgatttatt acattgggaa acaaaaacaa ataattttaa aagatcctat ccctttaaaa
     2221 gagctatatc caacaatcaa acatgtttat gaccaaaacg gtgtactgat tggtcgaaga
     2281 aaaaatggag tcttgcgttg taccgcaaaa gggatggggc gtttaatcag ctgaattgat
     2341 cattctcata acttgtattt tatcgttttt cgcttgcttg aaaatattgt cgagacttct
     2401 aaaggaactt agaatcacta ga
//
LOCUS       DAJSMI010000228         2412 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJSMI010000228 DAJSMI010000000
VERSION     DAJSMI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.232"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            33..1367
                     /locus_tag="ON430_002664"
     CDS             33..1367
                     /locus_tag="ON430_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220543.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1354..>2412
                     /locus_tag="ON430_002665"
     CDS             1354..>2412
                     /locus_tag="ON430_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220544.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLI"
BASE COUNT          797 a          367 c          415 g          833 t
ORIGIN      
        1 gtttttatta tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt
       61 tatatacttt tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag
      121 caatcgctct tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata
      181 gagaaaaaaa agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct
      241 taactacact attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa
      301 gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt
      361 tgacttgtgc attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag
      421 aaatacacag tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta
      481 tcattcaagc gttaaataac gatattacaa catacactat ggggttatta ggattggttg
      541 cgttgatttt tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg
      601 ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa
      661 aaacaccgat aagcttgctc cagtttcctt ggcgtatctt aggtatccaa atcttttttt
      721 cttcaatgat cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt
      781 taatcattgg cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt
      841 acgcaaaagt tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac
      901 gttatacaac atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag
      961 aaaggcctca cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt
     1021 ataaagctgc agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga
     1081 cagtaccagt atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg
     1141 actcttataa aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag
     1201 aaattacaac aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt
     1261 attgtttagt ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc
     1321 gatatgtgac taaaaaggag ataaacaaga agaatgaaaa taaataaaaa agtagtaata
     1381 ttgagtggat taattatagt tagtagtata tattctttca tctttttcaa aaatacacag
     1441 agtatttata ccactggaga tctttctttt cacttatctc gaatcaaggg gttatcgact
     1501 atttttacca gtccgattaa ttatgagaca ttcaattata ctggatatgg tgtaaattat
     1561 ttttatccct ttctgacttt ttttccagct gtaatgttat attggatgac aaaaaatttg
     1621 attgtaagct atattattta cgtttggtta ttgaacttgt gtacaacact cgtagcttat
     1681 cattatggcg agcggttttt aaaacaaaaa aaagcggcat ttttatttag ttgtctctat
     1741 attttctctg cttatcggac agtagacatc tactatcgtt ccgcaattgc cgaagctatc
     1801 gcaataacac ttgttatccc tgtattattt tatgcttatc aaatcatctc tggaaaagaa
     1861 gaaaagtatc cgtctgtaaa gctagcattg agtatgagtt tgctagtgta cagccatgta
     1921 ctatcaacat taatgagtac agcattaatt atcattttta tttttattcg tttggtttct
     1981 aaaggtttca agaatgcaga ttttattgct atatttaaaa aattatttag tgcggcagga
     2041 atgacattgg ttctgacgag tgctttttgg tatccgatgt ttgaacaaat gctctaccaa
     2101 aaaatcaaca aacctagcgt gacaaattta tatgctcatg catctaatgt gttcgatagt
     2161 ctgactgaag ctatgaacaa cgacttaact acttactcaa tgggactagt tggtctgtta
     2221 tctttatgta ttcccctgat tttatttaaa aaattaacaa acatagaaaa aaagatttat
     2281 tatggtacgt gtttgacttg gctagctaca actagtttgg tgccatggta tcttctgcaa
     2341 aatacgccag ccaaattgtt acaatttcct tggcggatct taagcttgca aataatattc
     2401 tcttctctga ta
//
LOCUS       DAJSMI010000229         2406 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJSMI010000229 DAJSMI010000000
VERSION     DAJSMI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.233"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            2..1147
                     /locus_tag="ON430_002666"
     CDS             2..1147
                     /locus_tag="ON430_002666"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR3220545.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1318..1776
                     /locus_tag="ON430_002667"
     CDS             1318..1776
                     /locus_tag="ON430_002667"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR3220546.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1935..2405
                     /locus_tag="ON430_002668"
     CDS             1935..2405
                     /locus_tag="ON430_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCR3220547.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          800 a          454 c          455 g          697 t
ORIGIN      
        1 catgcacatt caaacaaaat tgatccacgg aggcatcagt gaagatccca caacaggagc
       61 tgtcagtgta cctatttatc aaacatccac ttatcggcaa gatggcgtcg gccagcctaa
      121 acaatatgaa tattcaagat ctggtaatcc tactcgtttt gcattggaag aactgattgc
      181 tgatttggaa ggcggtgttc gaggattcgc tttttcttct ggtctttctg ggattcatgc
      241 cgttttttct ctttttcaag ctggcgatca tatcttatta ggagatgatg tctatggagg
      301 aacattccgt ctatttgaca aagttcttac taaaaatggt ctcgagtaca cgatcataga
      361 tacgagtaat cttgataaaa tagaacaatc catcaaaccc aatacaaaag cactttatct
      421 tgaaacacct agcaatccgc ttctaaagat tactgattta gaaaaaagtg ccactctggc
      481 tcatcaacat ggattgatag ttattgctga caatacattt gctactcctt attttcaaag
      541 acctttagat ctcggttctg atatcgttgt ccatagcggt accaaatatc taggcggaca
      601 cagcgatgta gtagctggtt tagtgacttc taaccataaa gatctagccg atcagatcgg
      661 attttaccaa aatgctatcg gagccgtact tggtccccaa gatagctggt tacttcagcg
      721 cggaatcaag acactaagtg ttcgtatgga ggaacatcaa aaaaatgctt ttgtggtagc
      781 tgattttcta ttttctcatc cagcagtaga aaaggtctac tatccaggat tacccgatca
      841 tgaactccat ggagttgcta agcagcagat gagcggcttt agcggaatga tttcttttac
      901 tttaaaaaat gaagaatctg ctattccatt tgttgaatca ctccaactat ttactttagc
      961 agaaagttta ggtggcgtag aaagtttagt ggaaattcct tctgtcatga ctcatgcgtc
     1021 tattccaaaa gaaaaacgag aagaggctgg cataaaggac ggcttgatcc gtctttctgt
     1081 aggaattgag tatggtcagg atctcataaa cgatttagct caggcatttg acagaatcaa
     1141 gaactagaag tcccaataaa cgacttagca gaaaaatcta aaaaatattt cagattgcta
     1201 caccttggaa acgataagtg ttagaatcaa gtagaaagac gtttttgtaa atttcactaa
     1261 ataattcttt agtgaaaagc atcaaaacga atgaacaaac agataggagt cttacaaatg
     1321 agaatgaatg tcgaaagttt taatcttgac catacaaaag tcaaagcacc ttatgtacgt
     1381 gtagctgatc gaaaaacggg tgcgcatggc gatgtcatca ttaaatacga cgtccgcttt
     1441 aaacaaccaa ataaagaaca catggacatg ccaagtctac attcacttga gcacttgact
     1501 gccgaactaa tccgcaatca cgcagattac atcgtagact ggtctccaat gggatgccaa
     1561 acaggctttt acttgactgt attgaatcat gaaagctacg aagacatttt agatgttctt
     1621 gaagcaacaa tgaaagatgt tcttgaagct actgaagtac ctgcaagcaa tgaaacacaa
     1681 tgtggctggg ctgccagcca tacactagaa ggtgcacaag ctttggctcg tgaattttta
     1741 gcaaaacgcg atgaatggtc tgaagtagaa gcatagtttc taaaagaacg taaagatgag
     1801 aagcaatata gctatcttat cttcgtattt tattaaatat ttattgtctg aaaagagttc
     1861 taaacgggaa aacaaatagc tagttttcag tttagcgctc tttttattat ataaaataga
     1921 aaggagaaaa ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct
     1981 attcattgaa aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg
     2041 gtctagcacc tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc
     2101 tggttatctt aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag
     2161 cagcagaagc ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg
     2221 taaatggaga aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg
     2281 tcactaaagc aataaaatgg aattttacta aatttttgat tgatagaaat ggcaaagtaa
     2341 tcgaacgatt tgcaccacaa acttctccat tgaaaatgac cgctgcaatt gaatccgttc
     2401 tttaac
//
LOCUS       DAJSMI010000230         2372 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJSMI010000230 DAJSMI010000000
VERSION     DAJSMI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.234"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            263..1426
                     /locus_tag="ON430_002669"
     CDS             263..1426
                     /locus_tag="ON430_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3220548.1"
                     /translation="MKKRKKIDWWILGPYLTLSMIGLLEVYSASSYRLLQADENTKSL
                     LLRQLIFIFLSWSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGA
                     QRWISLFGIQFQPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPK
                     IAGALMILSIAWVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNH
                     AYERIATLRDSFIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIF
                     SIITEELGLIGALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVG
                     SIAGLMPMTGVPLPFVSYGGTSYLILSLGIGITLNISSKIQAEELPLYRPEKQ"
     gene            1518..2315
                     /locus_tag="ON430_002670"
     CDS             1518..2315
                     /locus_tag="ON430_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3220549.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSVPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVIKAISQQLKDVTSIFKLPKAVGKLLGSIQTNLPESVLLDCGMDFL
                     KDNNKKIDTLSVPVDGSWDFNDNTPSGSVLELDLTKNQEAIKKFLNN"
BASE COUNT          725 a          396 c          493 g          758 t
ORIGIN      
        1 caaacatagg ctgttgccca gaaacgtcga aagtcgccaa tgggtaaaac agaaactgtc
       61 ggattaaggc tttttccaat agctaagaat gttttcttta cgttgtatag tgctaaaact
      121 cggacgatga gatgatgcta ggtcagaatt cttgttcgaa aaggtttctg accttttctt
      181 tatatttttg tgactgactt atttgatgag acaaactcgc tcctcacctg acaagtggat
      241 tgctgtgtga ggaggagatg cggtgaaaaa aagaaaaaag atcgattggt ggattttggg
      301 tccttatttg actttgtcca tgatcggtct tttagaagta tacagtgcca gttcttatcg
      361 tttattgcaa gccgatgaaa atacaaaaag tctattattg cgtcaactaa ttttcatatt
      421 tttgagttgg agcgtgatct tcttagctcg ttcagtcaaa ctacactatt tacttcaccc
      481 taaaatagca ggatacggtt tagccttatc gattttcttt ttagtattag taagaatagg
      541 gatattcggt gtcactgtca acggcgcaca acgttggatc tctctgtttg gcattcaatt
      601 ccagccttct gaactggcaa atcttttttt gattttttat ttaagctggt tttttcgtga
      661 cggaaatagt agcccaaaag atctaaaaaa accattcctg attacagtag gtataacttt
      721 tctgatttta tttcagccaa agattgctgg agcattgatg atcctttcga ttgcgtgggt
      781 catattttgg gcagcggcgg ttccatttaa aaaagggatc tatctaatcg ttactttttc
      841 tgcattgctg attggagcag caggcggggt attatattta ggaaataaag gttggcttcc
      901 acagatgttt aatcatgctt atgaaaggat cgcgacttta agagattctt ttatcgatag
      961 tcatggtgcg ggctatcaaa tgacccattc gttttatgca ctatataatg gtggcatatt
     1021 tgggcgggga ttaggaaata gtatcacaaa aaaagggtat cttccagaga cagaaacaga
     1081 ttttattttt tcgattatta cagaagagct tgggctaatc ggtgcattgt gtgtgctttt
     1141 cttattgttc tcattatgca tgagaatctt ttgtttaagc agtcgctgta aaaatcagca
     1201 agctggattg tttcttttag gattcggaac tttgctattc gttcagacga taatgaacgt
     1261 gggaagtatc gcggggctga tgccaatgac tggtgtgcca ttgccatttg ttagctacgg
     1321 aggaacaagt tatctgatcc tttctttagg aatcggaatc acgttgaata tttcttcaaa
     1381 gatacaggcc gaagagttgc cgctctatcg acctgaaaaa caataaagag aaactgagaa
     1441 actctcagtt tgaattatca taaagtacaa tcttttattt ttcaagcgag aggttctttt
     1501 agaaagagga attcgcaatg aaagtatctc aaaaaatcat attatctatt ttgttactgc
     1561 ttacattagt agttggctat ttttcgattg agtttatcca tggattttcg tctgcaaaac
     1621 agagttcaac tgtgaaaaag gtagagccga aaagtgtccc taccacacta aatgtggctt
     1681 tgattggttc agatgctcgt tcgaaagacg aaaacggtcg ctcggattca cttctggttg
     1741 cacaatacaa ccagaaaaca caacaagcaa aactgatctc tatcatgaga gattcatatg
     1801 tcgatattcc aggttacgga atgaataaga taaatgcagc ttactcttat gggggagttg
     1861 atttattaaa tcaaacatta aaggaaaatt tcaaatttga agctccctat tatgcaagta
     1921 tcacatttca agattttatc gattgcgtta atgaactgtt tcctggtgga gtcaagatcg
     1981 atgcagaaaa atctctagat ttagatggcg tatatataaa caaaggagag caagtgatca
     2041 aagctatctc tcagcagctg aaagacgtaa cttcaatatt caagctaccc aaagcggttg
     2101 ggaaattact tggtagtatc caaacgaacc ttccagaaag tgtgcttctg gattgtggta
     2161 tggatttcct caaagacaat aataaaaaga tcgatacatt atccgtacca gtagacggca
     2221 gttgggactt caacgacaat acgccttccg gaagtgttct ggaattagat ttgaccaaaa
     2281 accaagaagc aatcaaaaaa tttctgaata attaagtaaa gaaaataaaa gaagttagaa
     2341 ataacaatct atgttatgtt ctgacttctt tt
//
LOCUS       DAJSMI010000231         2350 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJSMI010000231 DAJSMI010000000
VERSION     DAJSMI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.235"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..544
                     /locus_tag="ON430_002671"
     CDS             <1..544
                     /locus_tag="ON430_002671"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3220550.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="ON430_002672"
     CDS             complement(1017..1589)
                     /locus_tag="ON430_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3220551.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="ON430_002673"
     CDS             complement(1605..2210)
                     /locus_tag="ON430_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCR3220552.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          740 a          469 c          358 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta
//
LOCUS       DAJSMI010000232         2311 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJSMI010000232 DAJSMI010000000
VERSION     DAJSMI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.237"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(100..1425)
                     /locus_tag="ON430_002674"
     CDS             complement(100..1425)
                     /locus_tag="ON430_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3220553.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMKDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            1875..>2311
                     /locus_tag="ON430_002675"
     CDS             1875..>2311
                     /locus_tag="ON430_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3220554.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAVRIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVY"
BASE COUNT          697 a          472 c          381 g          761 t
ORIGIN      
        1 aaattttgac tggttcgttg ttcattgtcg gcatcaattt cttcagtatg atcagataaa
       61 taaaaccctg cccactttaa acgtccacga tcattgtaat caatcatttt taattccccc
      121 atttccgccc gcatgaccac caacaagtgt gcttctagta atgcttctag cgccttctaa
      181 cattgaagaa gcatgtacaa tcgacgtaaa accatatttt tgccgaatct tgtcaataat
      241 tttatctaaa ttttcttcat caatttgctt ttgtgggtca ctaaataaat ccagctgaag
      301 cgaatcggtg tacacaagct tgctataggt tattccaaca tgccttactt cttgtccttc
      361 ataatactta tcaaacaaga aaagacaata attctgcaat tcctttgtat tatctgtgat
      421 agggatcttc atctggtgtg aaaaccctgg tctcgtttct aagatcgacg tgccaatgtg
      481 taaatggaca caacctgttt tacagttatg ctgcctaatg cgtatcgcta cttgttccgc
      541 catttctttt accaccagct cgatttcatt tcttctagta taatctcgtg gtaacacttg
      601 tgaattgcca tatgattttt cctttgtcgg ttctggtggg atcgctatat ctgttcgatc
      661 gattccgtta gcatggaaat acagctgtaa tccaatcacg cctaatctat tcttaatcgt
      721 ataaggattc cagttagcaa ggtctctaat gctcaaaatt cccatttggt tgagcctttt
      781 tttcattcgc gaaccaattc cccaaaagtc cttcatttcg ggaatattcc atactttctc
      841 aggaacgttt tcgtacgtcc attccgcaat aaacccttca ttatgctttg cttcattgtc
      901 taaggctagt ttagccaaaa ggggattatc gccgacgcct accgtagcga tcaatcctag
      961 ctcttcttta atacgttctt ggatcatttg agcgagcttc ttccttcgct gacttcgtgt
     1021 tccttcagtc gtaaaaagat tcagtgatcg ggtcactttt aagattgatt catcgatcga
     1081 gtaaatcagt agatcttcat cagccacata tcttctgaaa atattattta cctgcatatt
     1141 tcgcttgata tataggttca tacgtggtgg aacaacgtgt agtgttttag gaaatggttg
     1201 tggtaagtca cgtggtctac tcacatttgt aataccatac cgcttttttg cttcaggaga
     1261 agaagctaat atcaatcctg aaccagtatt atcagctcga ctcataacaa caagttctgt
     1321 tgttaatgga tctaaatttc tttctataca ttcgacactt gcgtaaaagc ttttcatatc
     1381 gattaaaaag taatcattta ctggctcttt tgaataatcc atcataaata acactccatt
     1441 ctttcacatt atacaaacat atgttcgata ttgtaaagaa taaatagcta aatacataaa
     1501 aaaagaagca taggtatgct atcaccctgc ttcttctttt gatttgtatg atcaatttat
     1561 tctatcactt tactaattaa tttgaaaatc taataaaaac tctcactcta aaagagtgag
     1621 agactgcaaa ttagagtacg gacttttttg gagttccgtt ctttaatatt aacaaatgaa
     1681 taaaatcctt acaacatttg agaataactt atcagtagac acttacaaaa tattctcata
     1741 ctatttcctc cgtcattagt ttttgtctat ataaggtata ccctaactag acacgaccac
     1801 aacattgaaa aatctggttt tatcgtgtat gattagggtg tattttaaat agttgcagaa
     1861 agtaggtaaa aattttgaga gttgcataca tacgtgtctc atccatcgat caaaatgaac
     1921 aacgccagat tgaagaaatg aaaaaattcg gtgcagtgag aattttcatc gagaaacaat
     1981 cgggcgcaac gattactcat cgacctgttt tccaagaagc attggatttc gtccgagatc
     2041 aagacatttt tattgtggaa gctattgatc gacttggaag aaattacgac gagatcatcg
     2101 cttcagtcaa ttacctaaag aagaaaaacg tacagctaat catcaccagt ctaccgatca
     2161 tggctgaagc aattggcaac cctctcctgg ataaattcat taaggatctg atcatccaaa
     2221 ttttggccat gatcgctgaa caagagcgta ccgaatctaa acgacgccag gcgcaaggca
     2281 ttaaaattgc gaaagcaaat ggagtttata a
//
LOCUS       DAJSMI010000233         2299 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJSMI010000233 DAJSMI010000000
VERSION     DAJSMI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.238"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(1..556)
                     /locus_tag="ON430_002676"
                     /pseudo
     CDS             complement(1..556)
                     /locus_tag="ON430_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            577..1779
                     /locus_tag="ON430_002677"
     CDS             577..1779
                     /locus_tag="ON430_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HCR3220555.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
BASE COUNT          807 a          370 c          402 g          720 t
ORIGIN      
        1 aaattgtttt aagggtttct tgaaagccta ccgtatctcg tttaacgaaa taatagcgta
       61 ataattgaca aaaatgatac gcagttcgca attcttcgtt gtagcttaac agctcattaa
      121 cgatttctgt ttgactaatt ggtcgtttaa acagtcgatg ataatgccgt ttgagtgcct
      181 cccatgtcgt ggaatctttg agtaagagtt tccaatatcg cttgagccga cggtacttct
      241 tctgattttc agagtgtgag gttcgaaaac gattcatgat ctggacacgt aattgattga
      301 agctgcgatt gatgtgttgg acaatatgaa agcggtcagt tacgatctcc gcatttggaa
      361 agaccgtttt aagcaactga caatagctgg cattcatgtc catcactagg tacttgacct
      421 ttaaacgggc ctttctagga aagcgcagga agtattgagt tagtttgtaa agtctgcgat
      481 cttcaagggt cgtaaatagc tgattggttg tcccatcaac acaaataaaa ctcatggaac
      541 cagaagtggc aacattgtgt acttttcttt gcaatcttgt gtacaaaact attgcaaaac
      601 acagcgcata agaatcctta caaaacagta gatgaagcaa cccaattaga tcttgttata
      661 gttgttaatc agctcttaac gggttacgat tcaaaatggg tcaacaccct atacttagat
      721 aagtcacttg aatatcagaa cataattcag gctttttcac gcacaaatcg tcttaatggt
      781 cctgaaaaac cgttcggaac cattcgttat tatcgtaaag cacatacgat ggaacagaac
      841 attgaagagg ctattgagtt atactcaggc aaacaagcaa aagggttatt tgttgaaaaa
      901 ttatataata acttagaaaa gttgaacgat atattcgaag aaattaagtc cgtatttaat
      961 atttctggca tagaaaactt cagtcattta ccggaggttc caggggaaat taagcaattt
     1021 gtaaatcttt ataatcaact aatccattgt tatgaatcag cgatgataca gggattcaca
     1081 tgggcaaaat cgacttatga aatccaggat tatgaaccta taacagtagt aatcacagaa
     1141 tatgaattta atatcttggc acaacgttac agcgagatag agagagaacc tagagacgga
     1201 aatgatagta tagcgtatga cattgattac caattacctg aaggtagtcg tagtcgtatt
     1261 aattatgagt atttaaacac aaacttcaaa aaatacatga atataaaaga agaacgtgat
     1321 tcgaatttaa ttcaatcatt gttaaacgac ttacatcgca catttgcgca attaccagtg
     1381 gagttacagc catatgctga gatgattcta aatgatgtaa ataatgggga attagttatc
     1441 aaagaaggat gggaatttac tgattacctc aatgaatatc agcttagtga agaggacgat
     1501 tttatccaag atttagtgga ttcaaccaat attaatgagg cattattacg tgagatgttg
     1561 gatttacgct taaccgaggt aaacattaat gaatatagtc gatttgataa attaaaatca
     1621 agtgttaacg taggtcattt cagtggatat atcgagaaaa atttaggtaa aatggtaata
     1681 ccaatcaagg taaatatgct aattgacaga ttattgcgtg catttttatt agaagatgta
     1741 ttcgatgtta cagagtacat aaagaattac tttaactaga gtacacctac atcttatagt
     1801 acataaaaaa attcatcttt tttagtttaa taattcacca aaaaggtgag taatgccatt
     1861 tttgaaaatt gagcttgttt tatgtctaca tctgtatccg tatataatcc aaaggattat
     1921 aaactcagaa gagtctttat tttttctgaa tataactact gtatcaaaaa caatcgatta
     1981 tgagacaaaa caaaatgacc tcttttattt aagaaaggag gtcatttttt gtatcataaa
     2041 ttgtattaaa atcaatgtat cactttttaa tcaattttaa gaccataaat tgcgtactaa
     2101 aagaaattct gcaattcaaa ataaagcatt ttaagccatt ttcaaattta agtgcaccca
     2161 tctacccgtc aaatcacttt attttccact ccagtagttt cctaattcaa ctgtgtgcaa
     2221 aacgatttct tctaaacaaa tcaagacaaa cttataaacc ttcatcccag caccataatt
     2281 atttaaatca taggcattt
//
LOCUS       DAJSMI010000234         2297 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJSMI010000234 DAJSMI010000000
VERSION     DAJSMI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.239"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            106..1968
                     /locus_tag="ON430_002678"
     CDS             106..1968
                     /locus_tag="ON430_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3220556.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1968..>2297
                     /locus_tag="ON430_002679"
     CDS             1968..>2297
                     /locus_tag="ON430_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3220557.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          826 a          341 c          452 g          678 t
ORIGIN      
        1 taatattagt taaaaagcta taagttgcac ttagttaagt gcaaacattt catattgtcg
       61 ttagtttaga aaacgcttat attagcaatg taaggagaga tgataatgca gcagctaaca
      121 aaaagagaaa ggcagctttt gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa
      181 aagttggctg aaaaagaaaa tgttagtgtt cggacgataa aatacgatct agatgatata
      241 agagagtggc tgaatgagcg gaagcaagtc ctatatagca aacgtagtca gggaatttgg
      301 atgaatatta gtagctctga tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt
      361 tttgaaatat ttgcagatca aagtctccga atcaaacgtt tactaattat gcttttagta
      421 acaaaagaag ctattactgg aatgaaaatg gccaacgcat tagatgtcag caaagatacg
      481 atcatgaatg atttagatgt gctggaaaat aattttctta aggaaggatt gaccttgaat
      541 cgtcaggcgc gaaagggttt ttggatcata ggagaagaac gtctagttcg attaacgatt
      601 gaagagattc ttcaaaaaga gttcacggat tatgacattt ataaattaat gtcattgtta
      661 ttgaatggcg gtgagactga atactttgaa atgtattctg ctactgctac acctatccaa
      721 gaggtattta atcaagtaat cattcgaatg cgacacttac tggaatttga aaatctagag
      781 aaactgaatt acgcggaatt actaaatatt ctgattcgag tgacaattgc taccgttcgc
      841 ttacgcaaag aggctacgat tggtcgttat caattggttg ctgaacaaga attagacaaa
      901 aacgatcttt cttatcgcgt catgcagcag gtctttgatt attatcagtt gccactgttc
      961 gaggatgaat atcattatat ctatagtgat acattcgaca cgattcacca gcaagacgtc
     1021 atgaaactag ttgagaatgt gatttataaa gtaagtaaag caatcaacca ttctttttat
     1081 caagatccac aattgttgac gaatctgtat gctcatttat ccatgcgatt atcgagaaag
     1141 aaaaaatttg tcaatgaata taatcctttt aaagaagata tcaagcagaa atacccggaa
     1201 ttatttgatg cgatcaagca agcagttcga caagaaataa gtgaacaaaa tattctgatc
     1261 aatgattctt tcgtggcata tattgcctta cactttttgg tttcctacga aagagaagaa
     1321 gatctacgaa gcgttagagc cgtctacatt tgttcaacag ggttgggtgt gactagtctg
     1381 atcaagcaaa agatcaatga agaattaaat aatatcacta tcgtggcatt tgcttcagta
     1441 ttgaatgcgc aagaaatgat tgctaccaaa gatcctgatt tggtgattag tatttttcca
     1501 attaatggca tcgatcgtcc tttcatcaaa gtccatccat tacccactga acaggacttg
     1561 aaaaaaattc aacaaataat aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat
     1621 aacttgaatc ttagagaaac gtccatcaat aaagatactt tagaagactt tagcaaggaa
     1681 ttgattttga aagcttatac gatttatgaa cgtttacttg tcattttgga agcagaattg
     1741 atccaagaat atcgagaagc ttttttgctt catgtattct taatggttca tcgtatcaca
     1801 tttgatcaac aatatatggc agaaggtcgt caagtagacc agattatttt agacaaagga
     1861 acactggtag gaaaaatcga agcagttttt gcagaaaata atttaggaat caatcagtca
     1921 gaaatcagtg cattattctc atatataaaa gaaggagcgt caagttagtg aaaatgtcag
     1981 acgaggcttt aaaaattatc gaggaaagtc aaaatcggga aaagttaaaa caagtgattg
     2041 atctaattga agagcgatta gtagaacatg ctattgtacc aaccgagttg cagtggacca
     2101 ttctaattaa tcacttgaat gagatgctga aacgttctca aagaaaggaa acgattcccg
     2161 tagtcgatcg ggatttgttc aaagaagtat ccaaagaagc cataactatt tctgatgaaa
     2221 tagttagaag tatcggtaat ttaaccgatg atgaaatata tgttttgtcg attcattttg
     2281 aaacagcaaa aaataat
//
LOCUS       DAJSMI010000235         2276 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJSMI010000235 DAJSMI010000000
VERSION     DAJSMI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.240"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            8..526
                     /locus_tag="ON430_002680"
     CDS             8..526
                     /locus_tag="ON430_002680"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3220558.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            654..1337
                     /locus_tag="ON430_002681"
     CDS             654..1337
                     /locus_tag="ON430_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCR3220559.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1646..1719
                     /locus_tag="ON430_002682"
     tRNA            1646..1719
                     /locus_tag="ON430_002682"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1680..1682,aa:Arg,seq:tct)
     gene            1878..2237
                     /locus_tag="ON430_002683"
     CDS             1878..2237
                     /locus_tag="ON430_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCR3220560.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          762 a          353 c          457 g          704 t
ORIGIN      
        1 gaaaacgata cctgattaca aaaaaataac ttttataagt tctataacgt tgattatatt
       61 tttaattctt attcttctcg tcgtcttggt tccccgaatt tttggtttga ctccttatat
      121 tattgaggac aaagaaatga gtccatatta tgcacaagga aatctgatat atgtacgacc
      181 gaaaaataca aatgagattt tagtcggaga tattattaca tattatgaaa attcaggaaa
      241 aaaaatctcg acgcgtcgcg taatagccgt tgaaaacaac cacgaagatt tttatacaaa
      301 agctgataca aagacttata ttgagcctgg agtagtaaaa aagagaaata ttatcggtag
      361 tcccatcttt cagattccat atattggact agtcctttca aaaacggcat ttgcatggat
      421 tcaggggtta ttctttacga ttgctttttg cctaacagga atcacagcat ggaatacctt
      481 tgtagagttg aaaaagaagc ggagacgaac acagaaattt ttgtaagaat gttaaaaaca
      541 taaatgtatt ccagagatcc aacgactctt ctgaacatga aacacgagaa aacactcatg
      601 cagcttgtag atttagtcag ttgttcgtat aatgtgtgaa gaggaggagt agtatgcgca
      661 atgaaatgat gcatcgtcct gtagtcaaag aagaactatt ggattttatg cgtacccagc
      721 aaaaaaaatt accgggtgag ctaggcaaac tggaagaaga agctcatgta gcagaagtac
      781 cagttattcc ccatgaaaca gttgtatttt tgaaattttt acttggacag cttcagcctg
      841 aacgaatctt ggagatcgga gcagctatcg gtttttcatc aagtgtcatg gccacgacaa
      901 tcggagaaaa cgctcatgtt acgacaattg atcgtttcga tgtcatgatc gagaaagcta
      961 aaaaaaacta tgaacgtcta ggactgacag ataaagtgac tttgcttgaa ggtcaagcag
     1021 cagatatctt gccggagttg tctggtccgt atgactttat ttttatggat agtgcaaaat
     1081 caaaatatat tgaattttta cctgaatgct tacgtttgtt aagaaaaggt ggcgttttga
     1141 tggtagatga catcttccaa ggaggaacga tcttgctccc agatgaagag attccccgtg
     1201 gcaaacgagc tatccatcgt aaattaaatg aatttcttcg tgttgtgatg gatcatcccg
     1261 atttgacatc gacaatcttg ccattaggtg atggtgtgat cttaatgaca aaggaatcag
     1321 aaacaatcga gttgtgatac atgattatgg cagcctgtag ataatcgatt gaatcgaggg
     1381 ctcaattcga acttgatgta aaagtttcta tatttgtaag tcaattaaag gatacaggaa
     1441 atctaaataa acaggagtag catgaagtca atgtagctcc tgttttcaat tgataacctt
     1501 agaacagctg aaggagaatg tctcgtttta aaagattgat ttagtagttg ggccaaaata
     1561 attcattatt tgctaattaa ataactattt cgtattgaca gaagcaattt aattatctat
     1621 aattattgat gttccgctga ttcttgtcat agtagctcag ctggatagag catccgcctt
     1681 ctaagcggac ggtcgggggt tcgaatccct cctgtgacgc taaaaaaccg ttagaaactg
     1741 aggtttctaa cggtttttct ttgttttttg tgccaatatt gtttgttttc aaaggcaaat
     1801 aaaataaaaa aacatcctta tcgagtacaa ttataaggag caaaaaaata aaactcatca
     1861 gaaaagagga agaagatatg acgatactat ctagaatttt agtgactcta gttgcaattg
     1921 aattttttta tatcatgtat atcgaaacgg tgagaactga ttctgacacg acaagtaggg
     1981 tttttaaaat gagtaaagaa gagttaagca gaaaatctgt tcaaacattg tttaagaacc
     2041 aaggagtgta caatggacta ttaggagtgg gactacttta tggagcttat ctttcttccg
     2101 catctaaaga aatcacaagt atgctattaa tatctatttt ttttgttgca ttatatggca
     2161 gtttgactag cgatcgttgg attttactca aacaaggtgg gctagctctg ttggcattgc
     2221 tttctatttt cttataaata tagaaaaaat aagaatttat ctaacaggaa actttg
//
LOCUS       DAJSMI010000236         2271 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJSMI010000236 DAJSMI010000000
VERSION     DAJSMI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.241"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..142)
                     /locus_tag="ON430_002684"
     CDS             complement(<1..142)
                     /locus_tag="ON430_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3220561.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..407)
                     /locus_tag="ON430_002685"
     CDS             complement(183..407)
                     /locus_tag="ON430_002685"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3220562.1"
                     /translation="MKKKISTIFFTNKQRYGATKIHQVLLKEGISVSLKHVQKLMKQL
                     NLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="ON430_002686"
     CDS             complement(548..802)
                     /locus_tag="ON430_002686"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3220563.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="ON430_002687"
     CDS             complement(995..1270)
                     /locus_tag="ON430_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HCR3220564.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="ON430_002688"
     CDS             complement(1242..2195)
                     /locus_tag="ON430_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3220565.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
BASE COUNT          678 a          417 c          330 g          846 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata c
//
LOCUS       DAJSMI010000237         2268 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJSMI010000237 DAJSMI010000000
VERSION     DAJSMI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.242"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(335..652)
                     /locus_tag="ON430_002689"
     CDS             complement(335..652)
                     /locus_tag="ON430_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3220566.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(653..907)
                     /locus_tag="ON430_002690"
     CDS             complement(653..907)
                     /locus_tag="ON430_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3220567.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLIILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1167..1385)
                     /locus_tag="ON430_002691"
                     /pseudo
     CDS             complement(1167..1385)
                     /locus_tag="ON430_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1945..2031)
                     /locus_tag="ON430_002692"
                     /pseudo
     CDS             complement(1945..2031)
                     /locus_tag="ON430_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          738 a          413 c          334 g          783 t
ORIGIN      
        1 tattaaaagt tacgtcaatt ttgattaaaa atatactact tgaacctctc atgagtaaag
       61 tcacgaaatt tttgggaata gagcgtactt gatatcttat tttttcacta atagcagtgt
      121 ctcttattca ccaagcgatc cccgtagttc ctacggttct taatttttct ctagactaat
      181 gattgtatct ttagaccttc ggccataaca tggagcaaaa taaaaaggta ctaagtctta
      241 tatatcaatc ttcgagtaac cgacggttaa caaccatatc gagcggcatt aatcacccac
      301 ctgtagagga tgagcgactt ttgcctaatt attgttaaaa gcctagtttc atttgtttag
      361 attggaaaat acgtcctaca agtatctctt ttttttcatg gtcgatccga taaaataagg
      421 catagctttt gccgatcacg atccgatatg ttgggatatc cattccatag atttcggaaa
      481 cttcctcatg catagttgga aaatttttgg ttagttctaa agccttctgg atcgcaccta
      541 caaatctttt tatgctggct ggagaaagag caagctcatt ttcccattct tctattatcg
      601 tttcaaggct ggtctggaaa cttttagaat aaactagcgt gtactctttc atctattcac
      661 tccagccgta ttttccgaaa gcttttacca tttcaatttc tgaaactgtt aaacctttct
      721 caaagtcttg gaaactttct tcaatcaatt tgcgatcgtg ttcttttgct atttgagtag
      781 tatctaatat gattaagttg ccttcttgtt tggcttggat agtgtctccg ctttttaatc
      841 caagagagct tggaaggatc actcccacgc tattgccgat ctttctaacc tttagttctt
      901 ttcccatgtt atcacacttc ttttagtttt tgataaataa attataacat gttataacga
      961 caattgccaa cggtgtttct taagggattc cactccatat tattattgga caaaataagc
     1021 cacttagaac gctgaaaaca tatacaaacg atatcaaaat acgagtgaac tactcaccat
     1081 acacttccgt taacatccat ttaagtaggt atttttatta tatatagatt taacatatgt
     1141 cggtcggttg attgatatag ctaaagttat aactcaaccg aagccgattc atctacacga
     1201 ataaagttgc gagtgttctc ggctaattca taaaaattgt tatgtgttcc atatagcaat
     1261 actggaataa ctttattttc ttgaatctca taagcaactc tatacacggt gaccgcatac
     1321 ttaaatccat tagaccaatt acctgataaa cctccagttt tttgctctta cttaaatgga
     1381 tcaatagcaa tttcatcaca aacctataga ggatgagtga cttctgccta attacgttaa
     1441 aaaatattga aagtaactct atgtagcaaa aaatatctag aatttcattt cctcaaaact
     1501 gatcgatatt gtatctaaaa atatatacta cctaaaatag gtagtatata ttaaaatatg
     1561 tgcttttgtt agaaaaatca atacataaat tatcatatct gtatatgtca tttaggtatt
     1621 tcagttatgt tataatagtt ttgctgtctg ctaccccggc ctaatctggc aaggggagga
     1681 ggagatataa tgaaatacct ttttgatttt gtttttgcaa tcaagccatt tgcagaggta
     1741 actgcacact acatctgcaa gtggcttgat tgcaaaattg ccaaaaaaga cagtaagcct
     1801 accagttaat tctataatat agaaacgtaa aaaaaacccc atcagcctac acgctgatgg
     1861 ggtttgatga tataatgaaa tacctctttg ttactcataa ttataccata attagtcacg
     1921 aatgcaacca atagaaaaaa ctacttagaa cggtagatca tcgctaataa caatgcattg
     1981 tccaccaaat ggattttgtg aagcattaaa tacattgtta ttaattgtcg ctgcttttaa
     2041 ctaggcctct ccccttctat tataaaagaa gatatatatt atttcttaat tacctactac
     2101 cttaaaccaa aatttaagta ctacctaatt taagtagtac ttaaataatc taaaaaatag
     2161 ttatcccaat tgttatgctt ttataatacg attatttgta acaaaatgaa tattatataa
     2221 ttaaacacta ccatatttag gtagtgttta attattagca aaacaaaa
//
LOCUS       DAJSMI010000238         2264 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJSMI010000238 DAJSMI010000000
VERSION     DAJSMI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.243"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            79..1125
                     /locus_tag="ON430_002693"
     CDS             79..1125
                     /locus_tag="ON430_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR3220568.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="ON430_002694"
     CDS             1474..2106
                     /locus_tag="ON430_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCR3220569.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          844 a          307 c          454 g          659 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaa
//
LOCUS       DAJSMI010000239         2253 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJSMI010000239 DAJSMI010000000
VERSION     DAJSMI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.244"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..564
                     /locus_tag="ON430_002695"
     CDS             <1..564
                     /locus_tag="ON430_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HCR3220570.1"
                     /translation="IKKTNKKTPMILLIIGIIFTTVGFSLSSSNEANDNNKQESSSSD
                     TQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSYYSITPATEQNTAFTKYISGKTYTF
                     EGTVIESMTTRIAIIADKEYDDKSWNDISATPKVSYVIFAKDLNNADTFTKGDKVTFT
                     GEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            677..1312
                     /locus_tag="ON430_002696"
     CDS             677..1312
                     /locus_tag="ON430_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220571.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            1611..>2253
                     /locus_tag="ON430_002697"
     CDS             1611..>2253
                     /locus_tag="ON430_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HCR3220572.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTW"
BASE COUNT          883 a          353 c          388 g          629 t
ORIGIN      
        1 atcaaaaaaa caaataagaa aacacctatg attctcctta ttatcgggat tatttttact
       61 accgtaggtt tctcgctttc ctcatcaaac gaggcaaatg acaataacaa acaggaaagc
      121 tcatcatctg atactcaaac tactacatct aacagcagca ataaaagcct aactcaattt
      181 gaagaatttt tagagacaaa taatcatgac tggggtgctt tcttagatag ttattattct
      241 attactccag ctactgagca aaatacagct tttactaaat atattagtgg taaaacatat
      301 acttttgaag gaactgttat cgaatcaatg actactagaa tagccattat agcagacaaa
      361 gagtacgatg ataaaagttg gaatgatatt tctgcaacac ctaaagtttc atatgtaatt
      421 tttgcgaagg acttaaacaa tgctgatacc tttacaaagg gcgataaagt aacttttaca
      481 ggagaaatta gttcaagagg atcgaacata gaaaaatcct atgctcaatg ggatatgatt
      541 aatagtaaag taagtaaaaa ataaatgaac cacgccccac taagagttag attgtgcaca
      601 ataataaaac ctatacaaaa gagtgccatc aggagtcaca atgcttgtaa aggaaatgac
      661 aaaataggag gtaactatgg ataatagcaa tgaaaaacta accaaaaaag aatatcttga
      721 attaatagtt gaaggcggta ttggagcaat tccttatatt ggtggcgttg tgcagtcact
      781 ttattttggg tctaaaaacg agaagcgttt caaacgcatc gagaaattct acaaaaattt
      841 aaacgaatca ttagaaaaca ttcgcgatca aattccagaa gatgttttca acttagagaa
      901 caaggaccaa ctcattggaa tattcgaagg gataaatgac gaggtagaaa aatcaaaggc
      961 acaaaataaa atagatatgt ataaaaatct ttataagaat tgtttacttc gaattaacaa
     1021 cgcttcatgg gataacgagg agtattatct tcttgtactg aatcaattaa catcgattga
     1081 gattcaattg ttagcatgct taatgagtcg tggaaacgaa aatttcactg gaaatatcag
     1141 catgtctggc tattcacaag agctaataga tggtagctta aatagattat ctgattttgg
     1201 tctgttagaa aaaacgatta gctccatagt attaggtgat gtcggcaaac aaaatatggg
     1261 ctacaaaata tctcatttag gaattcaatt tattaactca actcttactt aagttttctt
     1321 tctaatatga catctagacc tatcagcaat gcaacaataa aaaaccagac ataccaataa
     1381 tcaacaaata atctaaactc tggaagttca gttattcttt taaaaatcac tttaatcata
     1441 ataaacacct ctgattggat gatctaatac acatccaatt ataccattaa aacgacaaat
     1501 agcacatcct tgcgccggca agcaaaaagg atgtgcaaaa aataataaaa cccaaacaat
     1561 gggcttcttt atagttccta ttgtatcaga gaaagagagc tgattcaatt atggcaagat
     1621 tagtcaaacg tggaaatagt tggcaatacg aaatttcata caaaaaagat gacggaaaat
     1681 acacgaagat aagaaaatca ggatttaaga caaaaggaga agcaaaagat gccgccaacg
     1741 aattagaata taacttgaac aaaggcctta aaggggatcg caaaaatcta ttattatcag
     1801 attactttga ggattggatg caactttata aagaaggaac agtatctcct atcacttata
     1861 gaaaatacga agatacgtta atgaacataa agaaatatat gccagcggta ttgatttctg
     1921 atttagatag agtgggatat caacgctttt taaataagta tgctaaagac catgtaaaat
     1981 ctaccgttat taagtttaat aaccatatta gagcgtcgtt gaaagatgcc gtagaagaag
     2041 gattaattcc gtttgatcca actagaaaag cagtaatcaa aggaaaagat tcattgaagc
     2101 caaaagaaga taaatattta gattatgatc aatttaaatc tttaatgaaa ctcgtagaag
     2161 aaaacctttc tgcgcagtac tcttctccta tgctcgtgtt agttgctggt gccactggaa
     2221 tgcgatttgc tgaacttcta ggattaacat ggg
//
LOCUS       DAJSMI010000240         2246 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJSMI010000240 DAJSMI010000000
VERSION     DAJSMI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.245"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            84..1697
                     /locus_tag="ON430_002698"
     CDS             84..1697
                     /locus_tag="ON430_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3220573.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          703 a          399 c          444 g          700 t
ORIGIN      
        1 aataaacaat aattttccgg cacaattatt tgctgttttt taccataaca tagtacgatc
       61 aacgtagaaa aggagatgtt tttatgaaag ttgttgtcat tggcggtgta gctggcggtc
      121 cttcatttgc cactcgtttc cgtcgattga atgaagcgca cgaaatcatt atctatgaac
      181 gcggagaaaa tatttcttat gcaagttgtg ctttgcctta ttatttagga ggtgtgatca
      241 ctgaccgtga ctcgctgatc gaacgtacac cagaaatatt gaaaacaaaa aacaacatcg
      301 atgtctttac taaacacgaa gtaacagcaa tcgatccttc tactaagcga ttaacagtta
      361 aagacctatc cacaaatgaa gaaataaaaa cagattacga taagttgatc atctcttctg
      421 gtgctagacc agattatccg gatattcccg gagtttttga agcagaaaac ggctttgtcc
      481 tccgtagtgt gacggatgcg gatcgaatca aatcgttcct tgaagaaaaa aatccacaac
      541 atgtcgtcat tcttggtgca ggtgttatgg gtctggaatt agctgagaat ttcaagcatc
      601 gcggcttaga cgtgacttta atcgatcaat tgccacaagt cgctttccct tatgatccag
      661 aaattgctaa tttagtttat gacaaattgc tgaaagaagg attagccgtt catttagaaa
      721 caagagttac tgagatccgt gataaaggtc gagaaatagt attatcagat ggctccgtcc
      781 tttctgctga tatgctaatt tttgctgttg gtgtttctcc gaagaatgaa gtggtgaaag
      841 caaccggcat acaattatca gatacaggtc agatcattgt tgatgaccag ttacaaacca
      901 atctgccgga catctatgcg attggcgata ttatcgaaac gactagtgta gtgactggtc
      961 agccgatcca aagtatgctt tccagtgcgg ccaatcgtca aggacatatg ctggcagata
     1021 ttttaaatgg tacgcctatg cgctatcgcg gatatattgg tgcaggtgtc gcaaaaatct
     1081 ttgatcatac agcaagttat gcgggaatga cagaacatga attaaaagca tcaggcataa
     1141 caaattataa aactgttttt atcactccct ttgaccatgc ctatttctat ccagaagcta
     1201 caagattaaa tctaaagctg atttttgatg cagatagcgg tcgtatttta ggtggacaag
     1261 catttggaga aaaaggtgtc gataaacgga tgggagaact ttctgtagca atcaccggaa
     1321 acttgacagt ctttgatttg cctgatttgg agttgcctta ctctccaccg tattctacta
     1381 cccgtgaccc gttgaatata gctggttatg tcgcaatcaa tcaaatgacg aatatcgtag
     1441 aaacgatcaa agcaagtgat atacccgaaa acgatttgaa agaagcgttc tttttagata
     1501 tacgtgaacc taataaagca ccttctggca gtatctcggc aacaaaaaat attccaatga
     1561 atgagttacg agatcgaatc aatgaaatcc caaaagataa aaaagtttac attactttca
     1621 gaagaggatt gaatacttat acttctgccc gaatcttggc aggtttgggt atcaaagcga
     1681 ttttgattga agaataaaaa aacgaccgcg gtgggtagcc gcggtctagg agttattttt
     1741 acttgaggag taaaaatgaa aaagtatata gggttgttgg ttggtatgta tatagaatag
     1801 aagataaatg tgaagaaatt gtgcattatt tcttcacatt tattaggtta tagtttaaga
     1861 aagtttaaac attgcgcaat ttaattattt tagctgtttc ttttcataaa aaaacatgtt
     1921 gtgactctga gacaaatcat agtccatagt gatgtctcta gagtaactac gcctaatatt
     1981 acgaggcatc atattaatct atcagcacta gtacctaaac tagcatccct ttcgaatatt
     2041 ttttcttata taataaagtt tgctttcact cctctaatct caccaaatcg actggcttgc
     2101 gttgtgtgat actaggaact gattggttta gaacgacttt tctattgtaa tcatattttt
     2161 cgccatcctc gttgtcttct aatccttcaa tgaatgatcc ctaatgtgtg tattaaataa
     2221 tttaattttt ttgttcctat ttattt
//
LOCUS       DAJSMI010000241         2214 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJSMI010000241 DAJSMI010000000
VERSION     DAJSMI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.246"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            129..539
                     /locus_tag="ON430_002699"
     CDS             129..539
                     /locus_tag="ON430_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3220574.1"
                     /translation="MKKIFISISILVITGILGMLMYNNITSEKTYYTETKFGKISELV
                     KKNDRIILFVEQDGCSSCKLVKPIINNYAKENKGIVYSVTANKEKDYNVQSEKYHIEG
                     TPTLIYFKEGKEVYRTNTGFTEDEFQNTIKKVEF"
     gene            541..1065
                     /locus_tag="ON430_002700"
     CDS             541..1065
                     /locus_tag="ON430_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCR3220575.1"
                     /translation="MNKKEYLGIILIALASISIILFLYSNNRELKKANTMLQLEKQVL
                     IDNTDSFEYLNSITVDTFENRIKNKEDIFVYIGNSECADCSTFSKNLKNEVNNFPLKN
                     SLYLVEISRLHRNKKKWTEFKLKYGFDQTPAFILFKNGKVNSMIQWDEKKGLSSDSFH
                     RWLEENRNDIENLS"
     gene            1349..1519
                     /locus_tag="ON430_002701"
     CDS             1349..1519
                     /locus_tag="ON430_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220576.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            1533..2150
                     /locus_tag="ON430_002702"
     CDS             1533..2150
                     /locus_tag="ON430_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3220577.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNAIQQKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKSKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRKRYNVTVDQRKNKGKRDS"
BASE COUNT          869 a          263 c          421 g          661 t
ORIGIN      
        1 aaatgttgta gattatttat ttgggttaaa agggatgaag atttttataa cacatgataa
       61 agaggttatt tcaaagtgcg agaaagaata taccgtaaaa gacatgaaaa ttgtgaggag
      121 gcgataacat gaaaaaaatt tttattagta tctctatact tgtaataaca ggaattctag
      181 gaatgcttat gtataacaac attactagtg aaaaaacata ttatacagaa actaagtttg
      241 gcaagatttc tgaattagtt aaaaaaaatg atagaataat actatttgtc gaacaagatg
      301 gatgttcatc gtgtaaactt gtgaagccaa ttataaataa ctatgcaaaa gaaaataaag
      361 gaatagttta ttccgttaca gctaataaag aaaaagacta caacgtgcaa tcagaaaaat
      421 atcatataga aggtactcca acattaatat acttcaaaga aggcaaagaa gtttatcgta
      481 caaacaccgg ttttaccgag gatgagtttc agaatactat aaagaaagtg gaattttaat
      541 atgaataaga aggaatatct aggtatcata ctaattgcct tagcaagtat aagtattatt
      601 ctttttctat acagtaataa cagagaactt aaaaaagcta atacaatgct acagctcgaa
      661 aaacaagtct taatagacaa cacagattct tttgaatatc taaattcgat tactgtagat
      721 acttttgaaa atagaattaa aaataaggaa gatatttttg tctatatagg aaatagtgag
      781 tgtgcggact gctcaacttt ttcaaaaaac ctaaagaatg aagtgaataa tttccctctt
      841 aagaattcat tgtatttagt agagataagt agacttcata gaaataagaa gaaatggaca
      901 gaatttaagt taaaatatgg atttgatcag actcctgcat ttatattgtt taaaaatgga
      961 aaagtgaata gtatgattca gtgggatgaa aaaaagggtt taagttctga ttcattccat
     1021 aggtggttag aagaaaatag aaatgatata gaaaatctta gttaataatt cagaagagtt
     1081 agatcatatg tattgaataa attcagaaac aatttagtgc atagttaaaa agcatgtaag
     1141 ctattccttg gaaggtcaga aacttaaaag tttttgaccc tttttttatt cctcatttca
     1201 caactcttat agtggctcag ttaattaggt cagaaagtta ctttctcttg aggacaacag
     1261 agtatactta tttgtcctaa tatgatttta gcagtataat tgacttggtg aataggtcat
     1321 ttaagttgag cataatagga ggagtaaaat gaaaaaattt atttatcgag ttttagaaaa
     1381 tgacgaagtg gtggctattt ttaatgaaca acaatatgcg caagatttta tcgcttacga
     1441 aaagacaatt tctgataagc aatttgaaat tgaaaaagta gatatagctg attggttatt
     1501 gcaaccgaga gaattttaga ggttggttga aaatggctaa aattggttat gcacgtgtca
     1561 gtagcaaaga acagaactta gatcggcaat tacaagcgtt acagggcgtt tctaaggtct
     1621 tttcagacaa attaagcggt caatcggtcg aacgtccaca attacaagct atgcttaact
     1681 atattcgtga aggggatatt gttgtagtta ctgaattaga tcgattagga cggaataata
     1741 aagaattaac agaattgatg aatgccattc aacaaaaagg agcaacgctt gaagttttga
     1801 atttgccctc tatgaatggg attgaagatg aaaatttaag gcgtttgatt aataatttag
     1861 tgattgaatt atataagtat caagcggaat cagaacgcaa acgtattaaa gaacgacaag
     1921 cccaagggat tgaaatagct aagtcaaaag gaaaattcaa aggtcgtcag cataaattta
     1981 aagaaaatga tccacgttta aaacatgctt ttgatttatt tttgaacggt tgttccgata
     2041 aagaagttga ggaacaaact ggaattaatc gcagaacgtt tagaaggtat cgtaaaagat
     2101 ataacgtgac agtcgatcaa agaaaaaaca agggaaagag ggatagttaa tgagtacagt
     2161 tattttagct gaaaaaccaa gccaggcgtt agcctatgcc caggcattta atca
//
LOCUS       DAJSMI010000242         2148 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJSMI010000242 DAJSMI010000000
VERSION     DAJSMI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.247"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..1461
                     /locus_tag="ON430_002703"
     CDS             <1..1461
                     /locus_tag="ON430_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCR3220578.1"
                     /translation="TGLVTLAFGHVAQADNTTTAESSTSETMQKIEPKKDVYVIASDS
                     AFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAALQALESGQADGMIAG
                     MTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTVGAKVGTESAEFLSD
                     HESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQGQELETPIEKESGGK
                     YAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSETSSESSTADESTFLG
                     LIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRALRTIASIYVDIIRG
                     IPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIVRGGINAVPVGQMEA
                     SRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISAIGVVELLQTGKIIV
                     ARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            1463..>2148
                     /locus_tag="ON430_002704"
     CDS             1463..>2148
                     /locus_tag="ON430_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3220579.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFE"
BASE COUNT          694 a          397 c          459 g          598 t
ORIGIN      
        1 acaggacttg tgacacttgc gtttggtcat gttgcacaag ctgacaacac tacaacggca
       61 gagtcaagca caagcgagac aatgcaaaaa atagagccca aaaaagacgt atacgtaatt
      121 gccagtgact ctgcttttgc tccatttgaa tttcaaaacg caaagggcga gtacgaaggc
      181 atcgatgtaa aattgatgga aaacatcgcc aaactgcaag gatttaacat ccgaatggac
      241 taccgtggtt tctctgctgc tttgcaagct ttggaatcag gacaagcaga cggaatgatt
      301 gccggaatga ctgtaacaga tgaacgaaag aaaagctatg atttttctga ttcttacttt
      361 gatagcggga tccaattagc tgtcaaaaaa ggagatactt cgatcaagtc ttatgaagat
      421 ttgaaaggaa aaacagtcgg agcaaaagtt ggtaccgaaa gtgctgaatt tttaagcgac
      481 catgaatctg aatatggcta ttctatcaaa tattttgatg ctgctgatca attgtacgac
      541 gggttaaaag ttggcgcaat cgatgcaatg atggatgatt atccggtaat cggttatgcg
      601 atcaaacaag gacaagaatt ggaaacaccg atcgaaaaag aatctggcgg aaaatatgca
      661 tttgccgtta aaaaaggaca aaatcctgag cttcttaata tgttcaatga aggattgaaa
      721 gaattaaaaa gaacaggcga atacgataag attatcaaag aatatgtagc ggatggttca
      781 gaaacttcta gtgagtcttc cactgctgat gaatcaacat ttcttggact tatccaaaac
      841 aactatcaag cattgttgaa aggactttgg agaacaattc tcctaacttt gatctccttt
      901 gctttggcta tagtaatcgg tgtgattttt ggattattca gtgtagcacc aatacgcgct
      961 cttcgcacaa ttgctagtat ttatgtcgat atcatccgag gaattccaat gatggttctt
     1021 gcgttcttta ttttctttgg gttgcctggt atcgttggat ttacgattcc tgacttcctt
     1081 gctggtgtta tcaccttgac tcttaacgca agtgcttata tcgcagagat tgttcgaggc
     1141 gggatcaacg ccgttcctgt cggacaaatg gaagcttcca gaagtttagg tctttcttat
     1201 aatcggacaa tgcaaaaaat cattttgcca caagcaatac gcatcatgat cccatcattc
     1261 gtcaatcagt tcgttatctc attaaaagac acaacgatca tttccgcaat cggtgtagtc
     1321 gaattgctgc aaactggtaa gatcattgtt gcacgtacaa cacaaagtac gtatgtttac
     1381 ttgatcattg cgatcatgta cttgattttg atcacagcat tgacaaaatt agcaaaagtt
     1441 ttagaaaaga aggtgaagta agatggcaga aaaaatctta gttgagcatt tagtcaaaaa
     1501 atacggcgat aacacagtat taaacgacat caatgtatcc atcaacgaag gagatgtcgt
     1561 ctgtatcatt ggcccttcag gttcaggaaa aagtacattt ttgcgttgct tgaatcgttt
     1621 agaagaacct tctgacggtg aaattattat tgacggcgcc cacttgatgg ataaaagcac
     1681 agatatcaac ttggtgagac aacatatcgg aatggtattc cagcacttta acctattccc
     1741 tcatctttct attttggaaa acatcatttt agcaccgatg gacctgaaga aagaatctag
     1801 agaagaagca gagaaaaaag caatcaaatt actgcagaca gttggtctag aagataaaaa
     1861 agatatgtat cctgaaatgc tttctggtgg acaaaaacag cgtgtggcta ttgctcgtgc
     1921 tctagcgatg aaccctgaca tcatgctttt tgatgagcct acatctgcac tcgaccccga
     1981 aatggtcggt gatgtcttga acgtcatgaa aaagttagca gaacaaggta tgacgatggt
     2041 tatcgttacg cacgaaatgg gcttcgctaa agaagtggcc aatcgtgtca tgttcattga
     2101 cgacggaaac tttttagaag acggaacgcc tcaacaaatc tttgaaaa
//
LOCUS       DAJSMI010000243         2126 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJSMI010000243 DAJSMI010000000
VERSION     DAJSMI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.248"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            300..755
                     /locus_tag="ON430_002705"
     CDS             300..755
                     /locus_tag="ON430_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220580.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(917..2089)
                     /locus_tag="ON430_002706"
     CDS             complement(917..2089)
                     /locus_tag="ON430_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3220581.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT          678 a          424 c          385 g          639 t
ORIGIN      
        1 caattattat aacaagtaag taaacaattg aaaaggacat ttattgaaaa cggttgtttt
       61 ttacttaagg aggaaattta gtgaaaaaat tagttgcatt gtttttggtc gcaggaagtt
      121 tgttgagtgg gtgtggtgct actacgtcta ctactcaaag cacaagttca gctcaggctt
      181 ccagcagcag taccacgatc tccgaaagta cggcagattc atcgaaagaa caagaacaaa
      241 ttgaaaagaa aaaagaagaa gagaaacaaa aagcggaaaa agctgcacta gaaaaaaaag
      301 tgacagaagc ggaatctgct ttgtcacaag ctgaagcaaa tcctacaaaa gaaaactatg
      361 caactgcttc agcagctatc caagcgctac ctgaaaacaa acaagagttg acaagccgtc
      421 ttgctgcaat tgattccaca atcaaagcaa aagaagcaga agaagaacgg aaaaagcaag
      481 aagaaattgc acgacaacaa gcagaacaag cgcaacaaga agcaatcgca cagcaacaag
      541 cagaacaggc gcgacagcaa gcagcagctg aacaggcctc tcagcaggca caagccgcag
      601 ctcctgagca aaataatgaa caaactgtct atgtcacacc gaccggttca aaataccata
      661 cacataaatg tggaaatggt acgtataccc ctgccacttt aagcgaagct caagctcgtg
      721 gtttaacccc ttgctccaaa tgttatggaa actaattcaa aagttatact ataataataa
      781 aattcttctg aaacataaaa aaatagaagc tggcgttcca gatagtaaac caaaaatagc
      841 agtttacacg tccatttcgt cagcttctct cttttttcat ttagtctgtt ccctctgctt
      901 ttttggacat cttcattcat tctttctcaa tcaaatagta cacctctttt tcttttcgca
      961 gtttatatct tccttttttc aatttcattg aaaactgttt cgaatgttta tgttttacat
     1021 ggattttaag caggagctcc tcatgttgca agcgatagtt cctgcctttt aaatcagaaa
     1081 atctcactag tccgtattca tcagaagctt tcacaaatgg aactttttct ctagtaatat
     1141 agtgtttgcc gttgagcgtt tttacagaaa agaccattcc ttcgattggt cgtccgtttg
     1201 tgttctttac ataaaaagat aaaggatagt ttcgcttagc gatggccaaa gaatctaaat
     1261 aagcgatgag tagccaaact aagccgattg atccaagtag tataccgata aaccaaataa
     1321 aaaatttcca ttttttccat tgatcaattc cttggatcgc tttttttgcc tcttttgctt
     1381 gataaggaat acgatgcccc gtcacaagca gtcgatgtgt attgaccata taaggagtac
     1441 aggtaagcaa agtcacgaga tctctgcctt tttggatacc caaagaatct acttcatcag
     1501 gtaagaccac ttggattttt tctacttgat acgccaatgt ctttccattg atctggatga
     1561 aaaactggtc tccttttttc aacttaggca aatctgtaaa taattttgct tctgggagtc
     1621 cacgatgccc agaaagaacg gcatgggtgt ttttcccgcc agtcggataa gaactttctt
     1681 ccaataatga cgttcctttt tgcaaaaata tttccgttgt ctgatcgaat ataggtaagc
     1741 tgacattgat ttttgggatt tcgatgactc caagcatatg ttccataaaa aaggcattac
     1801 taggtaaatc ttttaatgct tttgcatcaa ctgcttggtt gaaactgcct atccctggtg
     1861 atacgttttg ctctgccaac tgttggtttt tctttgtcat tttttcctgc tgttttttta
     1921 tttcctctga attcttattc gatgcttgct tttgataatg gatgatcatt tgctgggcaa
     1981 gaaaatcatt cagtgaatct tctacaaaag gataagaaaa gatcccgatc ccaacgacta
     2041 aaatgattgc catgaagatg tccagtattc ttattctgct tttattcatc atgtccacct
     2101 attttctaat atgtatatga agaggt
//
LOCUS       DAJSMI010000244         2120 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJSMI010000244 DAJSMI010000000
VERSION     DAJSMI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.249"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(337..537)
                     /locus_tag="ON430_002707"
     CDS             complement(337..537)
                     /locus_tag="ON430_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3220582.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(842..2074)
                     /locus_tag="ON430_002708"
     CDS             complement(842..2074)
                     /locus_tag="ON430_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR3220583.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT          628 a          424 c          384 g          684 t
ORIGIN      
        1 ctgaattcca tactttataa aaatctttga taaatctatt cgaagggtca aatttaaacc
       61 ataattgtgt ttttccttcc atcagcataa aagttcacct caaaaagagt atacgaacga
      121 atgttctttt tgtgaaggcg gaatttaata tactgaataa tagaaaacaa gtacttgtgc
      181 caaattatgt gccaaaaaaa ttcgaattca acaaaatgta aacaaataga aaaccttcta
      241 tattaagatt tcctgcaata acaaacacaa cagaagacgt gtaatagtta gtcaggaacg
      301 tacaaataac ccctgtatcc tttgcggtac agggggttat tttgtataag tagacattgt
      361 agtttctaaa ctgctatgtc ctaaacgttt cgatacgcta agtatattta ctccttgata
      421 aagtaaaata gatgcatgcg tatgtcttag tccatcaata aacagtcgaa gagaaaatag
      481 ggaaaaattg attactaaag aaaaacttac taggtcgacg gtgtagttga ttttcatact
      541 tcaatagatt cgaattaaac gatttcgatt ggcttatacc agtcctatcc aacgaaagat
      601 atatcgttct taactctagc ggtataactt gaagctttac gtaaagccaa tgggaatgaa
      661 ctggagatga ctttttatta tttttagtaa tagctgatgg gttaatactt acaactttcc
      721 ttttcaaagt accatataaa aaaacggtgg ggttgtttag cgaaataaag gatctaagcc
      781 aatagcttag atcctttatt ctatgcttta tattattttt tgcatgtgtg cgaaagtata
      841 ctcaagccca atcgccattt cggaaaatcg gtactgtaga gccatcttca cggattccgt
      901 cgatatccat cttgtcagat cctaccatga aatctacgtg ggtttgactt ctattcaatc
      961 cagcttctgc taattcttca tcggacattt ctgtaccgcc tttcacactg aaggcataag
     1021 cagaacctag tgctaagtga ttcgatgcgt tttcatcaaa taatgtattg aaaaagatga
     1081 tgccagattg agaaataggt gaagggtcag gtacaagtgc gacttctcct aaacgacgcg
     1141 caccttcatc tgtatcaagc agttttgcta agacatcttc tccttgttcg gcagaaaaat
     1201 caacaacttt tccatcttta aaagtaaatt tcatacccga gatgatggtg ccagcataac
     1261 tcagtggctt tgtacttgaa atgtaaccat ctactcgatg actgtcaggt gctgtgaaca
     1321 cttcttccgt cggcatattt gccatgaatt tttctccacg agcattatag cttccagcgc
     1381 cttcccaaag atggttttta ggcaatccga taatgatatc tgtaccggga gcagtatagt
     1441 gaagggcaga aaattgttct cgattcaatt cttctgcttt ttttgctaat ttttcatcat
     1501 gcttcttcca tgctaagaca gggtcttctt catagacacg agtcgttttg aaaatctgat
     1561 cccataatgc ctcaacttgt ttttcttctg gcagttctgg gaagactttt gctgcccatt
     1621 gtttaccagc cgcagccacg acagtccaac tgactttatt tgcttgagtt gccttgcgta
     1681 aattcattag tgctttgcca ttcgctgatt gataagacgc tactcgatgg ctatctacac
     1741 cagcaaatgc atcgggatcg gcagaaacaa cgctgatcct gcttgctcct ttttcaagcc
     1801 attcatccgc ttgatcgatt ttagattgtg gtacattctc aatacgatcc gttgcagcat
     1861 gtaaaagaaa ttctctttgg atctggtcgt cagtccattg aacgataacc tcagcggctc
     1921 ctagtttata agcttcttgt gtgatcaatc gagcaagggg tgcctgttcc acgctgatct
     1981 gtaaaacaac tgtgtgtcct ttttctgtgg ctacgccagt ttctgcaatt aagcgtgcat
     2041 atttttttag aagttcatta aaatcagata acataataaa aattcctcct caccattttt
     2101 tctggtaact attagcatga
//
LOCUS       DAJSMI010000245         2105 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJSMI010000245 DAJSMI010000000
VERSION     DAJSMI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.250"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..912)
                     /locus_tag="ON430_002709"
     CDS             complement(<1..912)
                     /locus_tag="ON430_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCR3220584.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1340..1903
                     /gene="efp"
                     /locus_tag="ON430_002710"
     CDS             1340..1903
                     /gene="efp"
                     /locus_tag="ON430_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR3220585.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          708 a          373 c          410 g          614 t
ORIGIN      
        1 gtaaatgttt tttgataaat agcggtctgc tgcgtctgga aaaatcgtaa cgacacgatg
       61 accaggtggt aaacgacgaa tttctcttaa tgctgctgca aaagccgcac cactcgaact
      121 tccaactaat agtccttgtt ctcgtgctaa ctgcctagta tagttaaacc cttctacatc
      181 agagattgtc tcgatctgat tgattgaaag gggagaaaga aacggcggaa tgaattctac
      241 accgataccc tcaatttcgt gaggggctgg atcaccacca tttaacacag agccctctgg
      301 ttctacaccg attattcgaa tgtcgggata tttttctttc aaaaaggtag aagttccagc
      361 aaaggttcca ccactcccaa ttcctgctac aaaactatgg atattttctt tgatttcatg
      421 aaagatttct ggccctaacg tatgatagta agcagcagga ttatctttat tttcaaattg
      481 aagaggcaga tagctgtttg atatctcctc tgcaagtttt ttacttttgc tgatggcacc
      541 gatgattcct tgctcactag gagaatggat gatctttgct cccaatgctt tcatcaattg
      601 ttgtttttct aagctaaact tctctggaac aacaaaaacc gttcttaatt tgtatgtgag
      661 tgcggccaga gcgatcccaa ttcctgtatt tccggcagtt ggttcgatga tcgtcgtttt
      721 ttcattgatt tttccttggg caaatccttc tttgatcaaa taactgccca atctatcttt
      781 gatacttcct ccaggattta aatattctag ttttgcataa atagcagaac ctttaggcat
      841 agaatagtgc tgattgtcaa agcgataaag cggtgtggaa ccaatagctt ctgtaattgt
      901 cgagataatc atgtgggcct cctaaaaaaa gataataaaa aaaggagctg actattaaat
      961 cagctccttg aaagacatag agaaaaattg agtagataaa aagataagtt acctggtact
     1021 cattctatga agggcaatta atagacatga caaatagtat gctgctttta tgcaccatac
     1081 aacagcaacg attcaattgt ttttggttgc tgactgtcat gtgtatccct ccaagtaaaa
     1141 tttttattaa cgatagcatg aaaaaaaata aaagtcaaag gcttgaaaga aaaaaatatt
     1201 tcgatagcat cgtttacccg tgtttatttt tctgacaaat aggcatgaga ccttaaaaga
     1261 tagagaatac tctagttttc tggccggttg tctgatacaa taaagttgat tatgataaag
     1321 aaaagaagga gcaaaaataa tgatctcagc aagtgattta agagccggta tgactttcgt
     1381 acaagacggc aaactgatta aagttctgga tgcaagccac cataaacctg gtaaaggaaa
     1441 tacagttatg cgtatgaaac tgaaagatgt ccgttcaggt gcaacatacg acacaacgtt
     1501 cagaccagaa gaaaaatttg aaaaagcaca tatcgacaca aaacctgtac aatatttgta
     1561 cacgatggat gatacagctt tcttcatgga tttggaaaca tatgagcaat atgaaattcc
     1621 tgtagaaaca gtagaagaag aaatgaaata tatcctagaa aacatggaag taaaaatcca
     1681 atttttcggt agcgaagtca tcggcgttca attaccaaca actgtcgtat tacgtgttgt
     1741 ggaaacacaa ccttccatca aaggtgcaac agtcactggt tcaggtaagc cagcgacaat
     1801 ggaaactggc ttagtagtca atgttcctga cttcatcgaa acagatgaat tgttggaaat
     1861 caatacagca gaaggttctt atgtaaaacg tgcgtcaaaa taattcgcta acggattgat
     1921 aagaatttga aaacagagct tgacctcttg aaaggcagat catcggatct gcctttcaag
     1981 aggttttttg tggaatcagc aaattgaaaa ataaaaaagt atttgatttt atttatattt
     2041 tgaaagatga atggcaaaag ctagagaaag aaaaaactgc acttatttat gaagaaattg
     2101 aaatg
//
LOCUS       DAJSMI010000246         2083 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJSMI010000246 DAJSMI010000000
VERSION     DAJSMI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.251"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(41..1042)
                     /locus_tag="ON430_002711"
     CDS             complement(41..1042)
                     /locus_tag="ON430_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCR3220586.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(1201..1467)
                     /locus_tag="ON430_002712"
     CDS             complement(1201..1467)
                     /locus_tag="ON430_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3220587.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1457..1675)
                     /locus_tag="ON430_002713"
                     /pseudo
     CDS             complement(1457..1675)
                     /locus_tag="ON430_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1731..>2083)
                     /locus_tag="ON430_002714"
     CDS             complement(1731..>2083)
                     /locus_tag="ON430_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3220588.1"
                     /translation="QAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKT
                     EHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGF
                     SVSTEIKVLMGITA"
BASE COUNT          594 a          402 c          289 g          798 t
ORIGIN      
        1 atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt aaatctgatt
       61 catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc ataataacag
      121 atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc tctgaaatca
      181 aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt aaaattcccg
      241 atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc cttaaattgt
      301 tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa aagatagaat
      361 tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat tctattggat
      421 caaaaatatc taagttgtca tcaaaatctt tatcagttcc ggtaactttt ttgttaatta
      481 ctgaaggtgt cttgtaagaa atagaatcta aatcaaagcg ttttgattct aaattatttt
      541 gtgctttctc aattaaataa ttcacttttt ttgtgagaaa ccttctgata ttgtctttgc
      601 tatatgttcc ataaaaaccc ttatcttcta aagagtccct gtacttctta acttctatat
      661 actcttcagc caaaacttgt ggaactggtt catctaagaa aaaaacccct acctcgcctt
      721 tgctattttt ataaaagtaa tcaatttctg ctatagttcc cgatctataa ttatcaactg
      781 gagaacccaa cttatggcca aaaatcgcta ataatatatc actattttct acaatttgtt
      841 tattaataac actttgccca gagctattat ttatataagt aggaatcgca ttcgtttccc
      901 atcgaattgg catcaaaatt acttttgtat tatctgtatt ttctatattc catctatata
      961 tggtttcttc tacttcattc ctctttgctg ctatatcacc tggtgatgct ataaatactt
     1021 ttattagatt tatggttttc atttattgta cctttctctt tatgtctctt taaagtaatt
     1081 tttgtataaa atttattgac ttttacacta taggacaact tttatctatt tagtacaaaa
     1141 aggcccacta aactaattga ttagttagga gcctattaca ggtatatttt caactcgctt
     1201 ttaagcaaat tctactacca gctttttgcc taatgcccta gcgatatcat caagtttatc
     1261 gaaagatacg ttatcccctc tctcaattcg agcaatagta gattgagttg tatgtgcttt
     1321 ttgtgcaagc tccgcttgag ttaggcctgc ttcctcccgt gcatgataaa gagcaaccgc
     1381 agtttcgagt tttcttcctt cacggtcata tgcttctgca aaatctttgt ctttttgctt
     1441 ttcttcaatc aattttttaa tcatcatttg tgaaccactc ctctctaagt tcttttgcat
     1501 gtttaagttc acttattggt gttttctccg tttttttagt aaatccatgg gtaatgacat
     1561 accttccatt gaccacatgg aaatagatag ctctttggat gtttgatcct accttacttc
     1621 ttaattcgta caaattagtg tctaattttt tcacccattt caaccgttga gctactggtt
     1681 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
     1741 attcccatta ataccttgat ttcagtagac accgaaaagc cgaagagcgt tccatttctt
     1801 cggttctttt tatatattcc tcgaatggtc tccatgccct taatcgtgga agaggctgta
     1861 cggagacttt gataaaattt atttcgtcgt ttaataggtc gatggtcttg ttctattaaa
     1921 ttgttaagat acttcacagt tcggtgctct gtcttagtat ataaacccac actctgtaac
     1981 tttctaaagg cggagccaag agaaggtgct ttatcggtca caattgcttt cggctcacca
     2041 aactgtttat ggagtcgttt taagaaagca taggctgctt gcg
//
LOCUS       DAJSMI010000247         2078 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJSMI010000247 DAJSMI010000000
VERSION     DAJSMI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.252"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..219
                     /locus_tag="ON430_002715"
     CDS             <1..219
                     /locus_tag="ON430_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220589.1"
                     /translation="ATTASIKEEAQEQARKEYEQQLEELLNRTNQSTTESSMENSKES
                     DSSKAKEGSSDSTVQSNTSESSESNEVK"
     gene            219..>2078
                     /locus_tag="ON430_002716"
     CDS             219..>2078
                     /locus_tag="ON430_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR3220590.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAG"
BASE COUNT          756 a          336 c          404 g          582 t
ORIGIN      
        1 gccacaacgg cgtcaatcaa ggaagaagcc caggaacaag caagaaaaga gtatgaacaa
       61 caattagaag aattattgaa tcgtaccaat cagtcaacaa cagagtcaag catggaaaat
      121 agcaaagaga gtgattctag taaggctaaa gaaggatcaa gcgattcaac tgtacaatca
      181 aacacttccg aaagttcaga atcaaatgag gtgaaataat gcgtgttaga gaaacaacga
      241 agcgaaaaga caaacgattc aatttagaaa agaataggta tctttctcat ttctatcgct
      301 atactccctc aggagaagag ctaccagaaa aacatgacaa acgcatttcg aaaaagattt
      361 tttatagtgg acttgtatac ctccctttag agtatttagt aacaagagta ccagaagttt
      421 tcaaccgatt ggaaattgtt aagggaagct tacaaggaat aaatctgttt caattacagg
      481 aaatgccaca ggtctttcat tttgattcag ctggtttttg gaatccattt actcctacaa
      541 ctagtttggg tacacgttta gcaatttcga cagtttgtgt ggttccagct gtacttatgg
      601 gattgaaaat ggacatgtgg tttcgtcctc tagcagatgg acaaaaaggg gataatcgat
      661 taatgacctt aaaagaaatc caacagattt atccgcaaat acctgaaaca aaaactactt
      721 ttccaggtta tggaggtatt ccaatcacac attacaaaaa atattggtac attcaaacag
      781 atactgttaa tacgtgtatt gttggtacct ctcgatcagg gaaaggtcag attgaagtgt
      841 tagccaccat tgacaattta tctcgtgcag aaaaacaaag ttcaatggtc gttaatgatc
      901 caaaaacaga attatatgtt ggtagtaaag atgaactaga agctagaggg tatgatgtat
      961 atgcctttaa tatcaccgat ccactgcaat caatgagtga taatccattg gcactcatcg
     1021 tgaaatactg gaaacgagga gacatagata ctgcaacaca gttaaccaat actttcacaa
     1081 atacgattta ttatgatgcc aacgcttcag ataacaaata ctttaacgaa aacgcgcaga
     1141 aagctgtcaa tgcgcttatt tttgcgttgt tagaacaagc tgatcaaaca ggagattact
     1201 ccaaattgac accgaataat ctcgttgagc ttctttctga gattggcggt tttaattatt
     1261 atccagatcc agacaatgaa ttcaaacagt tgaatgcctt agatgaattt atgaatcagt
     1321 tgccacctgg aaacattgca aaaaaacaat atggggcaac gaaaattgga tctgataaag
     1381 cgaagggaaa tattttatca acggcaatca cgggattgaa tccatttact ctaacgaaaa
     1441 ttgcaaagat gacttctcaa tcaacgattg attataaatc ggttgggttt ccaaaatact
     1501 tagatttgaa attacatgaa agtcttttaa atcaacgagt cgtagtagaa ttttatcata
     1561 agggaaagaa atttcatgaa gaacgtgtca aagtaggcta taaaggattt tgtgaaatca
     1621 attttgattg tcatttagtt gcaggtgatc aagtttgttt aaaatacgat catcaaggaa
     1681 aagaatatgt ggcttggtat gaattttctc aaagagtatt gaaagacgaa gagggtaata
     1741 tcgtttatcg gaaaaaacga ggagaaaccc atttgccaga attagaaaaa gaagccgttc
     1801 taacattaga tgaggaaaaa agtaacttat tagtagagtc tattcggatg cactataccg
     1861 ataaaccaat ggctattttt atgcttacac ctgattatga tccatctaac catatcattg
     1921 ttagtatttt tcttagtcag ttatataaag aattatctac gcaatgtgtt aagactaaag
     1981 gagataaatg tcaccgcagg attcatttta ttttagatga atttgggaat atgccaccaa
     2041 tggataatat ggaaggaatc atgacggtca cagctggc
//
LOCUS       DAJSMI010000248         2065 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJSMI010000248 DAJSMI010000000
VERSION     DAJSMI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.253"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            289..696
                     /locus_tag="ON430_002717"
     CDS             289..696
                     /locus_tag="ON430_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220591.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            710..1111
                     /locus_tag="ON430_002718"
     CDS             710..1111
                     /locus_tag="ON430_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220592.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1113..1484
                     /locus_tag="ON430_002719"
     CDS             1113..1484
                     /locus_tag="ON430_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220593.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1520..1825)
                     /locus_tag="ON430_002720"
     CDS             complement(1520..1825)
                     /locus_tag="ON430_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220594.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDLYKVWDTEVVFLAIEDSLLIN
                     LYYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          768 a          280 c          351 g          666 t
ORIGIN      
        1 tttgagggga agtacatata gttgttgata tagttgctaa tattgttagc taatttgtta
       61 gtaaaactgt atacgaattt agatagtaaa aaaacagttt gttccttata tagaggcact
      121 tttactactg attaaatgtc agatatgctt acaaatatac ttataaatat gcttacaaaa
      181 ttagctaata tttccgttga caaagctttg ttactattgt taaactaaat tgactagcaa
      241 aagtgaaaca taaatttatt aaataaatgt aaaaattagg agaaatcaat gtcttataga
      301 gtacaattaa taatttcaga agatgtagaa agtcagcaat ttggcactaa tgttattaat
      361 aaagtaatta atccagctct ttctattaat gcgcctttaa ttcctactgc tttatcattt
      421 tctgtaacag ctgtggtgtc tgagatagag gatacagaaa agataaaaat agtagaaatt
      481 gaagttttaa acaagaatga aaaacaaatt ttttctacgg gtgaagtatc ggtaagcttg
      541 cctcctcaag ttaacgatat aaactttaat ataaacgcca gaaatgtctt ggttgaagaa
      601 gcaggagagc attatgctgt tgttaaattt aatggaactg agattggaag gcaaatcttt
      661 gatatcaagg tcaacaaacc agtggaaaaa aattaaggag acgatacgga tgcttgatat
      721 agtaccatca aatacacatc ggagtggtag cttaataaat tttgctgcga tttttgcttg
      781 cgcatcggta attctagcat ctccagttgt tgtggatgca agttctactc cgaaaactaa
      841 aaatgataat caacttgtaa ttactacaaa aaatgagatt aacacaattt caagtaatga
      901 tggcaaaatt tttgatgtga taaatacggt tttaaaaaga aaagctgaaa tcaatataaa
      961 atatgatgaa gatttaaact tgtatttctt tgttatcaaa acaacatctg aattgttttc
     1021 ctcagattac gatgttttgg atactctcga taatgtatta tctgactata aatatatggg
     1081 gaaatctgta gtagcaactt tgggggaata aaatgtttga ctttaatgaa tatttatcta
     1141 tttccaaaca aattgaatca gatgataaat ataattctaa agaatcttat agaagaactg
     1201 ctgtttcaag agcatactac agtgctttta aaaaatctga tgagtatttg aaagaaaatt
     1261 atgatattat ttataatgga agtagcggaa aaggaagcca ccaaaccgtt tggaatttgt
     1321 tttcaacagt taaggaattg aataccttag gaatacataa tagtgggtat agaatgttag
     1381 agaaaagaaa atgtgctgac tatattccta atgaaaccat tacgaaaaca gatatggcgc
     1441 tgatgaatcg agaagcagaa aaaataataa acaaactcac ctaatgaatt tcaagcgagt
     1501 ttgtttgtta tttttttatt taatctttgt atctttctgg atcaacgaaa gtatacttta
     1561 tatagtcata acgccgatga tcgctacgtg cgtccggcac gtcagtcacg atatcaaaca
     1621 aaaagtatac acccttcttc attctagttt tcgcagcagg aattttgaaa tagttcttat
     1681 tagaatagta gagattgatt aataagctat cttcgattgc taaaaagact acttcagtat
     1741 cccagacttt atataaatct ttgacaaatt tttctgatgg atcgtattta aaccaaagca
     1801 gatgttcctc tagctcgatt gtcatgtttt tcacctcaac gaaagtatac gaactaatgt
     1861 ttgttttgta aaggataaaa atagggtagc aaccggtacc caaactagta cccatttatc
     1921 aaaaacgacg aaatttatgt aaatagaaaa ataagaataa acattgatgt atcaacattt
     1981 atatatcttt agaacaaatg aaaagggtgg ttctcataag aaaaacatgt tcgcacttct
     2041 aggcaaacca ggatttgaag acctt
//
LOCUS       DAJSMI010000249         2048 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJSMI010000249 DAJSMI010000000
VERSION     DAJSMI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.254"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(52..642)
                     /locus_tag="ON430_002721"
     CDS             complement(52..642)
                     /locus_tag="ON430_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCR3220595.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            1165..1836
                     /locus_tag="ON430_002722"
     CDS             1165..1836
                     /locus_tag="ON430_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3220596.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
     gene            1833..1994
                     /locus_tag="ON430_002723"
     CDS             1833..1994
                     /locus_tag="ON430_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220597.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMKYVADEANRK
                     QRKLVGLDK"
BASE COUNT          728 a          325 c          356 g          639 t
ORIGIN      
        1 taaaattact tgtcatacta ttgagaagga gtatttaatt gaagagaatg attaggagaa
       61 aggatctccg tactttttat agtaatcata gaatccaagt ttgtatagac gaatcgtttt
      121 ttctcttatt tcttctgttt caaaatggac tgcttgaaga atcaagtctg tgaattctat
      181 aggagctgtt ccattcgcag taatcaaatt acgatctttc acagcttgtt gttctataaa
      241 atcttcttta ttctgataat tttccgattc taaccaagga aattgcgcat ttccggtgtg
      301 tttgtaacct tgcaataatc cgctctgagc taaataatca actgccccac aaatagcagc
      361 tactggtacg tctgttttta acaatttatc gattatttga tataaatgat gattttttat
      421 gtcccatgaa ttgccaccga tcataataaa aaggtcaact ttttcaggta tttgttcgat
      481 taagtaatcg atttttgttt ttaatcctcc aatggaagtg acctctgatt ggatagaagc
      541 agttttgttt tgccatccct ctttttggtt tagtgtgcta gtaatataag caccttccca
      601 atcggcatat tcctctaata gaaacgtaat cgcagttttc attttcaagc tctcctttac
      661 tatctatgac aaggacaaca gttttaacat acagacattc attaaatttg gatagcgaca
      721 gcgaaaacaa caattcttaa gttttagaca gagtatacag acagtcaact taattaaata
      781 cgaactaaat aaaaatgaca ggtaatcatt tttgtttacc agcattataa cattttgcat
      841 acatggatga caaaagaacc aattcccact tgcttgagta caatcttaaa agaaactgga
      901 aggcaaaaag taattataga aacgaaaaat aaatcatatc gggcactcag acatgaaaac
      961 aaccctagaa atatataact agtccaatga aaaaacagcg aatgccgtag aaaacaagga
     1021 ttctgcgtac attcatatct cattttgcga gcatatctgc tattactgtg actaaatgtt
     1081 aatatggtta aaatgaccgt tatatttttt atataaatat cgtataatta gtatgaatat
     1141 agaagaagga aagaaggtgc aatcttggaa cctaaagaca agtatttaga ttacgctagg
     1201 gtcaataaag ataaatttat tgaaacaatc atcaagggaa aaatccctga tgatgaaaaa
     1261 gatgcggtat tcatggctgg aagcccagga gcaggtaaaa cagaggttgc actaggttta
     1321 gctgaaaatt atgacaacca tgttgtgatt gatgcggact attttagagc acaatttcca
     1381 gaatacaatg gaaagaactc aagtgttttt cagaaagctt cttcttggtt agttgaacaa
     1441 tcattaaaat atgtactgga acatggctat tctttcatac tagaggatac ttttgctatc
     1501 cttagtgcag agaaaaatat tattcgagcc cttaaaaata actttcgcgt tacgattttc
     1561 tatgtttacc aagatcccaa agttgcctgg gatttcactc gaaaacgtga actagctgaa
     1621 ggacgtcatg tgccaaaaga aatgtttatc aatgcgtttt ttaagtccag agagaatatt
     1681 gaaaaagtca aaaatagaca tcctgaagta gtccttcaca taatgattaa ggattatcaa
     1741 aacgacattt ctgaagtgca ttatgcagca gataatattc aattagtact acccaataca
     1801 atatacttca aaagagttgg aggaggaatt acatgactga gaaagaacaa gtacaacaaa
     1861 tcgtgaagaa gtataataaa agtatcgctg atttatcaga aaacgcctca gcgaaagaat
     1921 ttaaaacagt aatgaagtac gtagcagatg aagcaaatcg taagcagcga aaactagttg
     1981 ggttggataa ataaattttt taaaccatat ccttgtaaag ttgagttttt tgttttaatg
     2041 aattaaga
//
LOCUS       DAJSMI010000250         2041 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJSMI010000250 DAJSMI010000000
VERSION     DAJSMI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.255"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            58..834
                     /locus_tag="ON430_002724"
     CDS             58..834
                     /locus_tag="ON430_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCR3220598.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            849..1052
                     /locus_tag="ON430_002725"
     CDS             849..1052
                     /locus_tag="ON430_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3220599.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1068..1406
                     /locus_tag="ON430_002726"
     CDS             1068..1406
                     /locus_tag="ON430_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCR3220600.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            1393..1572
                     /locus_tag="ON430_002727"
     CDS             1393..1572
                     /locus_tag="ON430_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220601.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(1615..>2041)
                     /locus_tag="ON430_002728"
     CDS             complement(1615..>2041)
                     /locus_tag="ON430_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220602.1"
                     /translation="KIKGMRPQLEVELNASYFAADMIYTKVIISNYSTEPAMLVNLEL
                     SSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSAISCYLAFEVGKINFN
                     KLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
BASE COUNT          752 a          295 c          401 g          593 t
ORIGIN      
        1 aatttagaaa aaagttgtta aatttgttta cgaatataaa agaaaggaat gagaaatatg
       61 aacacaccgc aaatttttaa tttcgaacaa aacgaagttc ggacaatttt agtaaatgat
      121 gaaccatatt ttgtaggcaa agacgttgcg agtgttttgg gttattcaaa cactaaagat
      181 gctttgtcgc ggcatgtaga tttggaagat aagatggggt cgcgaatcac gacctcaggt
      241 caatcaagag agatgacaat catcaacgaa tctggtttgt acagtttaat cttaaaatca
      301 aaacttccct ctgccaaaaa atttaaacgt tgggtaacaa gtgaagtgtt gccagcaatt
      361 agaaaacatg gaggttatct aactccagaa aaagtagaag aagctttgct taatccagat
      421 acaatcattc aattagcaac tcaactaaaa gaagaaagaa ctggaagatt aatcgcagaa
      481 caaaagattg cagagtacga acctaaaatc tcctatttag atagcatatt atcttctaca
      541 gattcagtaa caattagtca gattgcagca gattatggga tgtctccaca acagatgaat
      601 aaattacttc ataaactagg tattcagaaa aaagtcggta accaatggtt attgtgcaaa
      661 aaacacatga accaaggata cacaaaatct catacaactg agatcccgaa agccgatggt
      721 ggcactaaaa ttgtaatgaa taccaaatgg acacagaaag ggcgtctatt tatctacgaa
      781 ttactaaaaa aagaaggata ttaccctcaa atggatttag aggaaattgg ttagaaagga
      841 gttttagtat gactgacatt gcagaaatca ctcaacgaga tagagaaaaa atcaaagaat
      901 atgtcgaaag ttcaaagttc ttaacttaca ccatgcttgc tgaaagattt ggaattagca
      961 aaagctactt atctttaatt ttaaacggta aaaagacttc tgcagaagca aacagaatta
     1021 tagattcgat tatcactatg tacgaattgt aaagggagga acagctaatg caatatctag
     1081 aagcaaaaat cccaattcca gaaggctatg taattatctc ccaagtggat tatgaggagt
     1141 taaaaaaagc tgatgatact ggtagatgga tgacgttgcc agaagtgcta gaacggatta
     1201 acagaaaata tgattggttt acttctagag ttttaaagaa cccaagatat agaaatatta
     1261 tcgatatcga gaaaaacaaa aatggatttg tctattatcc agttgaagga agagacacat
     1321 atctattttt aagaagtaaa acacttgaat ttttagaaac aaatttttcg gaaatcttaa
     1381 ggaggcaagc ggatgggaaa atttaataga gcgctagtgt tcagcgcacc gctaatcatc
     1441 tacgctttag gactttgggg aagcaggcaa gcgttgatag gaacgattgt ttacatggtt
     1501 tggattttta tggggcttga tgaagctgaa gctgagtaca gagcgaaaaa gccaaccgag
     1561 ggggctgact aaaaatgaat agaaaagaga aactagaatg tatattacta ttactcagtt
     1621 taattctttc actaatttct ctattgggaa gtttgtattt ttgatatcaa caattttgtg
     1681 gatttgggtt ctatttagag taaatatcat ttttgtttgg tgattaagga gtttattaaa
     1741 attaattttc cctacttcaa atgcaaggta acaagatata gcacttttag gaggaatatt
     1801 tagcggtaca gcttcagagt atattctatt atcatcgact gatcctcctt tagcaattaa
     1861 tttcttaaat ggagtagctg accatctgcg gtttaagtta gcggatgaaa gttctaaatt
     1921 aactaacata gctggttctg ttgaatagtt agaaatgatt acttttgtat aaatcatatc
     1981 tgcagcgaag taagaagcat taagttcaac ttctagttga gggcgcattc cttttatttt
     2041 t
//
LOCUS       DAJSMI010000251         2002 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJSMI010000251 DAJSMI010000000
VERSION     DAJSMI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.256"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            224..790
                     /gene="amaP"
                     /locus_tag="ON430_002729"
     CDS             224..790
                     /gene="amaP"
                     /locus_tag="ON430_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR3220603.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            803..991
                     /locus_tag="ON430_002730"
     CDS             803..991
                     /locus_tag="ON430_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR3220604.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1004..1561
                     /locus_tag="ON430_002731"
     CDS             1004..1561
                     /locus_tag="ON430_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3220605.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1593..1835
                     /locus_tag="ON430_002732"
     CDS             1593..1835
                     /locus_tag="ON430_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3220606.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          738 a          307 c          407 g          550 t
ORIGIN      
        1 aatcttgaat aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa
       61 gcaattgtta tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg
      121 gtagcgcttt atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt
      181 agagttattt acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa
      241 aataatcggt gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat
      301 caatgcgccg tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa
      361 ttactacatg caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat
      421 catcttgcta gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga
      481 agatggcaaa ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca
      541 cgaagaagag ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca
      601 tatccatgtc aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt
      661 gatgcaagat atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt
      721 ggcagtcaca tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg
      781 agtggaatag gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat
      841 ctttgggggt cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac
      901 attgttgatc ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc
      961 aggaatagtt gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat
     1021 gaatacaaca aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg
     1081 tgaattgact tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt
     1141 agatggtcta ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa
     1201 cacagataac gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga
     1261 tctagatgtg atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga
     1321 attgatcagt actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa
     1381 tgttgcagac atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa
     1441 agtaacagaa gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa
     1501 atctgctgta ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata
     1561 agaagtaaga agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg
     1621 gcggtatcat tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca
     1681 tcgcaaacat cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt
     1741 ggggaccaac aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag
     1801 tattagtcgt ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg
     1861 aaatgaagaa caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt
     1921 tatatgaagt attctttatt ccttatgaaa aaatatcttt aaaaataaaa aaagacgaaa
     1981 ataaacggtt ttctataaaa ag
//
LOCUS       DAJSMI010000252         1996 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJSMI010000252 DAJSMI010000000
VERSION     DAJSMI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.257"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(69..1625)
                     /gene="rny"
                     /locus_tag="ON430_002733"
     CDS             complement(69..1625)
                     /gene="rny"
                     /locus_tag="ON430_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCR3220607.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT          515 a          442 c          338 g          701 t
ORIGIN      
        1 gttttttcgt gccttacaaa aaaagaagag ctgtggcttt tgccgcggct cttcttttgt
       61 attttttatt atttagcata atcagttgca cgagtttccc gaataacgat tactttgatg
      121 tgtccaggat aatccagttc ttcttcgatt cgtttacgaa tatcacgtac taaacgaaca
      181 gcgtctaaat ccgagatttc ttctggcttg accattaccc gaacttcgcg tccagcttga
      241 actgcgtaac ttgattctac accggcgaag ctgttcgaaa tattttccag acttcttaaa
      301 cgacggatgt agttttctaa tgattcactt cgcgcaccag gtcttgctgc tgagagcgca
      361 tctgctgcag ctacaaggac agagataaca gaagtagctt ccacatctcc atgatgagaa
      421 gcgatcgcgt tgattactac tgggttttct ttgtacttgg cagctaattc cgcaccgatc
      481 tcaacgtgag atccttcaat ctcatggtca agtgctttcc caatatcatg gagcaacccg
      541 gcacgttttg ctaattggat atcttcgcca agctctgctg ctaagatacc agctaatttt
      601 gcaacctcga ccgaatgctt caagacgttt tgtccataac tagttctgaa tcgcagacgt
      661 ccgatgatct tgattagatc tggatgaagt gtatgagcac cgacttcaaa cgctgcctct
      721 tccccgtatt cgcgaatacg ttcatccatt tctttacgag atttctcgac catttcttcg
      781 atacgcgctg gatggattcg tccatcttgt atcagttttt ccaaggtcat ccgtgcgatt
      841 tctcttcgga ttggatcgaa tccggaaaga acaactgctt ccggtgtatc atcgatgatt
      901 aaatcgatac cagtcagcgt ctcaagcgtt cgaatattac gaccttctcg tccaatgata
      961 cgacctttca tttcatcatt cggaagggta acgactgaaa cagtcgtttc tgaaacttga
     1021 tctgctgcgc agcgttgaat cgctaatgat aataagtttt tcgctttgcg atctgcttct
     1081 tctttggcac gctgttcaga ttcttttacc attagagtta actcatgatt cagctcttct
     1141 tcagtcgatt tcatgatgac atctcgtgcg tcctcttttg ataaaccagc aattttttct
     1201 agttcttttt gttgatcttc aatcattttt tcaacgtctt tttctcgctc ttcaattaat
     1261 tgctgttttg aactaagtgc ttcttcttta ctttcaagtg agttctcgcg tttttctaac
     1321 gagtcatctt tacgattcag cgtttgttcg cgttgaatca aacgattctc ttgagatttt
     1381 aattctaact tactttctct cagctcactt tcaacttcag aacggtattt ctgattttct
     1441 tccttagctt ctaacaatgc ttcttttttt agagtttctg cttctttttt tgcactatcg
     1501 atgattccag cagctgatga ttgtgcacca gctatttctt tctcgtgacg tgactttgcg
     1561 accataaacc ctaaaccaag accgacaatt aaaccgatga tagcgaggag aatgttgaat
     1621 accataaaat ccacctccat actatctttt tcatttttat catctataca gcttctatga
     1681 taaattattc taaactcgtc aatatgcatc ttcgcatata tgtgtacaaa aaacatgcac
     1741 aactttattc taatgtttgt accccgtagt gtcaacttta aataaacaaa gaaacggctt
     1801 tctacttatt cattaaacga tggtttttaa aataaatgac actagtttca tataggtaaa
     1861 cctttaaatt gagaaacttt tctattaaat tattctttgt agatgaacag atatttttat
     1921 tttatgtacc cactcgtata attaaataac tcacgatttc tttattcaat aaaaaaacgg
     1981 caggtcaagg agagta
//
LOCUS       DAJSMI010000253         1927 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJSMI010000253 DAJSMI010000000
VERSION     DAJSMI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.258"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            87..983
                     /locus_tag="ON430_002734"
     CDS             87..983
                     /locus_tag="ON430_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3220608.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            1082..1291
                     /locus_tag="ON430_002735"
     CDS             1082..1291
                     /locus_tag="ON430_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCR3220609.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1308..1580
                     /locus_tag="ON430_002736"
     CDS             1308..1580
                     /locus_tag="ON430_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3220610.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            1582..>1927
                     /locus_tag="ON430_002737"
     CDS             1582..>1927
                     /locus_tag="ON430_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3220611.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVI"
BASE COUNT          741 a          272 c          358 g          556 t
ORIGIN      
        1 aagagggctt ttatatatta atcacaaatc acttatcaca aatcacaagt gattaatcac
       61 aaatcacttg tgatttgtga ttcttaatga tacaatatta ctatacaaaa aaagaatggg
      121 gcgtagttat ggagaaggaa aaactaaaaa tacttgaaga attaagacgt attttaaaca
      181 ataaaaatga agcaattatt atcttgaata attactttaa aggtggtgtt ggaaagtcca
      241 aattatcgac tatgtttgct tacttgacag acaaattgaa tttaaaagtt ttaatgatcg
      301 ataaggactt acaggcaaca ttgacaaaag acttagcaaa aacttttgag gtagaattgc
      361 cacgtgtcaa tttttatgaa ggcttgaaaa atggaaactt ggcttcttct attattcatt
      421 tgactgataa tttagacttg atccctggca cgtttgattt gatgttactg ccaaaattaa
      481 ctcgctcatg gacgtttgaa aatgaaagta gattgcttgc tactctttta gcacctttaa
      541 aaagtgacta tgatcttatt attattgata ctgtaccaac gccaagcgtt tatacaaata
      601 atgcaatcgt ggcgagtgat tacgttatga tccctttaca agcagaagaa gaaagtacaa
      661 acaacattca aaactatatt tcctatttga ttgatttgca agaacagttt aaccctggac
      721 tagatatgat tggttttgtt ccttatttag ttgatacgga cagcgcaacg ataaaatcaa
      781 acctggaaga actgtacaaa gaacataaag aggataattt ggttttccga aatattatca
      841 agcgaagtaa taaagtaagt acttggtcta aaaatggcat tacagaacac aaaggctatg
      901 acaaaaaagt tttgtctatg tatgagaacg tattttttga aatgattgag cgaatcattc
      961 aattagaaaa tgaaaaagaa tagaatcaca aatcacaagt gattaatcac aaatcacttg
     1021 tgatttgtga taggtgatga taaaataaat agtaagaaga aatagaaaga agtgagcgat
     1081 cgtgggaaat ttaggcgcac agaaagcaaa acgaaatgat acgccaatca gcgcaaaaaa
     1141 agatataatg ggggataaga cggttcgtgt tcgtgccgac ttgcaccata tcataaaaat
     1201 cgaaacagca aagaatggcg gaaacgtaaa agaagttatg gatcaagcct tagaagaata
     1261 tatacggaaa tatttacctg acaaacttta aaaggagtga aaatgaaatg gcagttacgt
     1321 atgaaaaaac atttgaaatt gaaatcatta atgagttatc ggcaagtgtt tataatcggg
     1381 tattaaatta tgttttgaat catgaactag atactaaaaa tactcgttta ctagaagtga
     1441 atcttttaaa tcaattagaa gtggcacaag aagttgattt atttcaacaa ccatttgaag
     1501 aattacaagc tattcatgag tattggcggt caatgaatca atattcaaaa caaattttga
     1561 ataaagagaa agtggcttaa catggcgaat attactgact tcaccgaaaa gcaatttgaa
     1621 gatcgtttag aaaagaatgt tgaacgacta actaaaaata gactagcggt tgaatcgcca
     1681 accgcttttt tacttggtgg gcaaccaggg tcagggaaaa ccagtttgcg atcagcaatt
     1741 tttgaagaaa cacaagggaa tgttattgtc attgataatg atacctttaa acaacagcac
     1801 ccaaattttg acgaattagt gaaactctat gaaaaagacg tagtaaaaca cgttacccct
     1861 tattcgaatc gcatgacaga agcacttatt agccgtttaa gcgatcaagg ctataattta
     1921 gtgattg
//
LOCUS       DAJSMI010000254         1921 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJSMI010000254 DAJSMI010000000
VERSION     DAJSMI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.259"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            47..1216
                     /locus_tag="ON430_002738"
     CDS             47..1216
                     /locus_tag="ON430_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR3220612.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1483..>1921)
                     /locus_tag="ON430_002739"
     CDS             complement(1483..>1921)
                     /locus_tag="ON430_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220613.1"
                     /translation="GTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVI
                     YSSFSPNKDEITFTLKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSES
                     GINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT          677 a          328 c          297 g          619 t
ORIGIN      
        1 agctttattc tttctgtaaa aaagaaagga gactaaataa actattatga aaaaaggaaa
       61 acatgctaaa aaaagaaaat attcattttt aatgatcgtt gtcgtgttgt ttagccttag
      121 tgcgttctct acaggaacta ttcttggatg gcaattttac aataatgaac actctcagat
      181 agttgcccat gctcaatcag ctcaaaaaaa gttgatactg actaccaatg aacaacagct
      241 cgttgaaaaa atcgatcaac tattaagaaa aaatgattat attggctcaa tttacgtaaa
      301 gaaaaataat cgcatcatca tggaaaaagg ctatggctat gcgaataaac tcacgcaaca
      361 accaaatgat ccttctcttt attatcagat tggttcggtt caaaaagcat tgactactct
      421 tttgatttta aaacaagtcg agcgtgggca cctttcttta gatactaagc tggctcaatt
      481 ctatccacaa attgctggca gccagcaaat tactatccgt gacttgctgt atatgcgctc
      541 aggcttgcac cgaactgctt ctccaaccat accgatgact gatgaagaag tcgttcaatt
      601 tgcgattcag cacttaaaat ttacagatta tcagacctct cattatgatt cattgaactt
      661 taccatattg actggaattt tgatcaaatt aacaaatcaa ccgtatgaaa aattattgac
      721 cgatgatatc atcgaacctt taggattaca gcatacaaaa ttttatgacc aagtcaaaaa
      781 ttcatccaat catgcactat cctatcaaat gtctaaagag gatgattact ttaaaccact
      841 gacagaatct gaaacagaga tccataacga attagggact ggtaatatca gtatgtccgt
      901 ctatgattta aatacgctgt ttacacaagt tttatctgga aaactagttt ccaaagactt
      961 actcttctca ttatggcgtg aaaatgcgaa tggacatcca tatagtggtg gggtatatag
     1021 cggagataat tatatcctgt cgcaaggaaa tatcgactct tttgatacat ctgctatatt
     1081 tagcaaagat tcacaggatg ctatcatcat ggaaagcaat ataaaagctg acaaaactat
     1141 caaactgcct actgctgatc taagaaacca aatctatgac ctgatggaag gaactactaa
     1201 gttggcaaat agctgatatg atgaaaagat agcaaatact acttctttga atagcatttg
     1261 cttaaaaaaa gtactcgact aaacgtagga aagagtgtgg gacaaatcta aaaatcaatt
     1321 tttgctacag actttaattc caaataaaca gaacagaaag aggtgttctt ctttctgttc
     1381 tgtccaaatt attctaagat ttctctttac caacattatt tgacaaaggg cctattaaat
     1441 ctaaaatagt atctttagat tgctaaaata tgtgctagca aatcattcat ggcttgttct
     1501 tatttttaat tttatgcaat aaaaaagaag aacaattagc gtgattatag aaataaataa
     1561 tgagatttta gaaaaaaatg tgtatgaata cctgattgtt actttattta ttccactttc
     1621 actttttact gtaacaaaag gacctccttg ttttagtact gtagcatttt ttgaattaac
     1681 agtaaccgtt aatcctttat accggaatac aggtaaaact attgttttgc cttttgaaga
     1741 tttaagcgta aatgtaattt catctttatt tggagaaaag ctactataaa taacttgacc
     1801 atcttctgta acttgatggt ttttcaaaag caattgatct tttttatagc ctataggtac
     1861 ataatcaaaa ttctcaaatt cttcaagttt attttccatt tgttcattag aataagttcc
     1921 t
//
LOCUS       DAJSMI010000255         1918 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJSMI010000255 DAJSMI010000000
VERSION     DAJSMI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.260"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(243..857)
                     /locus_tag="ON430_002740"
     CDS             complement(243..857)
                     /locus_tag="ON430_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3220614.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1463..1840)
                     /locus_tag="ON430_002741"
     CDS             complement(1463..1840)
                     /locus_tag="ON430_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220615.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          634 a          384 c          293 g          607 t
ORIGIN      
        1 ctttttattt ttctgacact acatattctt ataactcatt tctcataact gtgaaacaac
       61 ttataaaaat aacaaacttt aaacaaaaca agcttaaaat caaagatgat ccatcttaaa
      121 ataactaaaa caaaaaaagt agtcgttgtt aatcctcatt aataatcgtt ttcttttttt
      181 caaacgctcc atttttatgt ggaacattat ttttttaagg aactttgtca acatgtggat
      241 aactattttc cacttgtgtc ctggactagc tgttttgctg taaaatacaa aacttgctgc
      301 agcaaaaaga tcgttgcaag agaggctgtt ccgcagatcg ctggatctag atttggcaaa
      361 gtcaagactg catcatgtaa attggcgtgg ttgtccaaaa ccaaatcagc aaattcaaat
      421 aatcgcaaac cactgctatg ttgcgatttg atacttcgtg acaaatcaaa tccagtaaca
      481 atgattaatg gatgaccatt ttctttgatc cactgagcta gttcgatgat tgccgggttt
      541 cttccttcat tcgaaagaag aagaaaaatg tcttctgatt tgacagaaag ctgatcaata
      601 attgcctgtc ctgcaccttc tagcgtttca tagacacctc cggctggatt ttcaatcaat
      661 atacttggta caagatccgg ggtttgatcc caaaattcaa atgctacacc acttagtgtg
      721 cggctagcat atatctgcaa tctaccacct tgattaattt gtttcgcgat ccaagtgcca
      781 gcttgaagca tcagcggctc ttctatcctt aatttctctt ccaattggtg ataatagtca
      841 aaataacata aactcattta tctctcccgc ctagctatac ttgacatgaa tagaaacagc
      901 tgcctaatct aaggaagtac ataaaaaaac agtagttaga cagtaacaca tgtggtcttt
      961 gctgtttcaa acacaaaaaa ggcattgaac gaatacgccc aataccttga catagcaaat
     1021 aaaacaacgt gcagaccatg gaaaatatct acataggggt agaattttca ccctagatta
     1081 ggtacaataa attaggtcga tgcactgcat tgactatggc aaaatgatcg gtcagggtgt
     1141 atgtgtgtga ctggtcattc cctgattctt agcgccaact aagggtcagg gagccttttt
     1201 ttattcagct gtttctatct gaatttattg taagtaatct cttatccaag tgcaagcaag
     1261 aaaatacaaa aaaacattgg tatgatttat atatatataa ataacataaa aaaaggaatg
     1321 gcttatcccg atacaacaag cattccttta aaaaagattc ttttatataa gtgaaaaaag
     1381 cttttttcca ccaattaaat aaatctctca tctcaatagt ttatccatta ttttatcttt
     1441 ttttgcgtat acctactaaa tattaccgtc ttcttctcgt tcttctactt tgttcgagag
     1501 aatctgtact agaaaaagta gttgtatgtc taccttttct gcctatccga gactttcttt
     1561 tcatatatgg gatataaatg attagataca gtacagcaaa aaataaagca aatacgcaag
     1621 cttcccaact acctaacgcc aattcaacca tcaaggtagc aaccccaacg gatgctgcat
     1681 accagataaa aatcagaaac gtttcttttt ttctgaccat gcgaaacatg ataaatagac
     1741 taataagcaa tagtggcaac acaaatatcc aattgcttcc ttccatcagc cacttgagta
     1801 tatcaagcag cgactgtgca acagcgacta attttcccat agtgaacaca cacctttttt
     1861 tataattaaa aaattcgcct ttttattcta ctatgaatct tctggcttgg ctttcatt
//
LOCUS       DAJSMI010000256         1906 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJSMI010000256 DAJSMI010000000
VERSION     DAJSMI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.261"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(644..934)
                     /locus_tag="ON430_002742"
     CDS             complement(644..934)
                     /locus_tag="ON430_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220616.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1142..1834)
                     /locus_tag="ON430_002743"
     CDS             complement(1142..1834)
                     /locus_tag="ON430_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR3220617.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          516 a          366 c          295 g          729 t
ORIGIN      
        1 aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag aaatctacga
       61 tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa ggacctatgg
      121 attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca tattgctttt
      181 aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt aggtttaact
      241 gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt ttacgactat
      301 gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa atcctacaat
      361 tccactacct gagactagta atgactagtc tctttttatg gcctctgatc cccccattga
      421 gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat ccctcaaatg
      481 agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta tatatagatt
      541 gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca tccatctttc
      601 caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt attctttcat
      661 cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca attttgggtt
      721 tccctaacac atgataggat ctcacaacaa attgctttct attattataa taacctgtaa
      781 cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg gaagcaatca
      841 aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact tgcaaagtac
      901 ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata ccattatacg
      961 aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt tttgtctatt
     1021 acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta ctaaaaaaaa
     1081 gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt gttaagaaag
     1141 attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta tcttccactt
     1201 catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt tcagcagttg
     1261 cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt ttttcttgtg
     1321 tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct gaaagttttg
     1381 cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt tcatcatttt
     1441 tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt agttgtgcat
     1501 ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga tttttttggt
     1561 tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa tcttgcaatg
     1621 tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct ttatctgtca
     1681 atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc tctccacccc
     1741 acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa
     1801 cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa tccaatactt
     1861 ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttt
//
LOCUS       DAJSMI010000257         1901 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJSMI010000257 DAJSMI010000000
VERSION     DAJSMI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.262"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(1..673)
                     /locus_tag="ON430_002744"
                     /pseudo
     CDS             complement(1..673)
                     /locus_tag="ON430_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            751..1790
                     /locus_tag="ON430_002745"
                     /pseudo
     CDS             751..1790
                     /locus_tag="ON430_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          553 a          403 c          398 g          547 t
ORIGIN      
        1 cgggaattct aaattcagta atatctgtta accatttttc atttggcttc tttgctttaa
       61 aattccgatt taataaattg gggtgtgcag gagaaatttc cccagcatac gaagaatact
      121 tttttcgtct agaaaattta acaaatagat tttcctcagt catcaatcta cgtacaactt
      181 tttcagaaag ctttttaccc atatttttta aagcttggtg aattctacgg taaccatacg
      241 cacagaagtt atgattgaaa atcgtgataa ttaattgacg ttctttgtaa tacttatctg
      301 gtttctcaag tgcttgtttt tgataaaaat agctgctttt aggcaactgg agctgttgaa
      361 gaagctcttt caaggtgaac tgatttcgta aggcatcaat caatatcgtt ttttcttgat
      421 ttgatagaca aagaagattg acgccttttt ctttttttag cagttcagcc gctccttcga
      481 gtaatgcttt ttcgagctgt aattggtgta cttgtttttg taagtcttct acttccttta
      541 aaagtagctc tttttgtttt tcctgatttt ttaaagtcat acgcaatgga aaatcctttc
      601 caagaaccgt gtttttccat tgatatagag taactctact aattccttca gattcggcta
      661 aatttttcct attatataaa ttgagttaaa ttgtattcgt ttttcctcct ataggcgtat
      721 actaaagaaa acacttgagg agtggggaac atggagacag aacgccaaat ccgtgaattg
      781 gaaaaagcga tgaatacgac agacaaccgc cggttgtacg agcgtttcct ggcagtcaag
      841 ttggttcttg aaggacacac ctggaaagaa gccgggcaca ccatcggccg cgacgagcat
      901 actgtcagcc actatgtgaa aaactactgc cataatggaa tccaaggttt agtctccaaa
      961 aaacaaagcg gacgaccttc acggctgaca gatgaacagg aagcggaact ggtccaaata
     1021 gtcgcctacc acactccgga ggaagtaggt ttcaaatccc gtgccaattg gacattggcg
     1081 attgtgtgtg acctgattct ccgggaatgg agttatggat atacgcaaaa aggcgtggcg
     1141 gacctccttc accggctagg actaagctgg acccgtccga cgtataccct caaaaaagcg
     1201 gattccaaga aacaacaggt atttttggaa gaaaccttcc cgagcctaaa aaaaaaattg
     1261 ctgaatagcg atattgacca cctcctattc gaggacgaaa ccatgatccg ggattaccaa
     1321 gccattggct gcacctggtt tctccgcggc caccagcgaa aaatccccac ttatggacaa
     1381 cacaaagggg taaaattgat aggcaccttg gattatgaag ccggtcggat catttgtaca
     1441 gaagaagggg catacgacgc agcgaccttc ctccgttttc tggagcggac gttgcgggaa
     1501 taccccaccg gaaaaatcgt gatggtattg gataatgccc ttatccacca cgctaagctg
     1561 atccagtctt ttcttgaaga aaacgctagg cgcctggcac tcatcttcct ccctccctac
     1621 agccctcagt tgaacctaat ggaaggcgtt tggaagtggc tcaaggaatc ggtcatcaac
     1681 aatgtttttt tgaccacgtc cagaaaatca aacagtcggt ccgcggtttc ttagccgatg
     1741 taaacgagcg tccccttgag gtgattgacc gactgtgcgt gcggatgtaa agtatttaat
     1801 tcaacctata tagttaattg tagcctatcc aaatcggaat tttaaagcaa agaagccaaa
     1861 tgaaaaatgg ttaacagata ttactgaatt tagaattccc g
//
LOCUS       DAJSMI010000258         1883 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJSMI010000258 DAJSMI010000000
VERSION     DAJSMI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.263"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..1031)
                     /locus_tag="ON430_002746"
     CDS             complement(<1..1031)
                     /locus_tag="ON430_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3220618.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1150..1791)
                     /locus_tag="ON430_002747"
     CDS             complement(1150..1791)
                     /locus_tag="ON430_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3220619.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVV"
BASE COUNT          635 a          425 c          327 g          496 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat cagacaacta accctactag cacagtaaca cttgcaacca acgtgactgc
     1201 agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca cagacgaagg
     1261 gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca tcgcatctgc
     1321 agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca ttacacctat
     1381 ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg ttttcttatc
     1441 taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag cattacccaa
     1501 aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga ctccaactac
     1561 tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca agagtatcga
     1621 tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg ctctcggatt
     1681 taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca ctaccagtat
     1741 cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca tatttcctcc
     1801 tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa gcaccgaaat
     1861 ccaacatagg atctcagtgc ttt
//
LOCUS       DAJSMI010000259         1866 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJSMI010000259 DAJSMI010000000
VERSION     DAJSMI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.264"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..320
                     /locus_tag="ON430_002748"
     CDS             <1..320
                     /locus_tag="ON430_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3220620.1"
                     /translation="NILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTD
                     EIERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNV
                     VTW"
     gene            332..1336
                     /gene="neuB"
                     /locus_tag="ON430_002749"
     CDS             332..1336
                     /gene="neuB"
                     /locus_tag="ON430_002749"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HCR3220621.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            1333..>1866
                     /locus_tag="ON430_002750"
     CDS             1333..>1866
                     /locus_tag="ON430_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HCR3220622.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDT"
BASE COUNT          675 a          240 c          376 g          575 t
ORIGIN      
        1 tcaatatttt aactttagct caatcattaa taaaaatata taatgataca catgatgaaa
       61 aatatagagg tgaaataaaa gttgtaggta tcaggccagg agaaaaatta tatgaagagc
      121 taatgactac agacgaaatt gaacgctcat atgaaaatga taaaatgtat atcattccac
      181 cacactatca tgctactgac gaactgtaca aagattatga aaaaattaaa atcactgcat
      241 actcttcaga tgcaacagaa ttaatgagtg gacaggaatt tattagaatc gcaaaagaga
      301 ataatgtcgt gacttggtaa ggagttttat gatgaaatca gaagtatatg taattgcaga
      361 aattggtgta aaccataatg gaagtctaga tttagcttta gaaagtattg ataaagctaa
      421 agaatgtgga gctaacgcgg ttaaatttca aacatttaaa acgaataagc tagtttccaa
      481 aaaggctcca atggcaaaat atcagaagga taatcttaaa gaggacagtt ctcaatttga
      541 aatgctaaaa aaattagaat tatctcaatc tgattttatt agaataaaag attattgtat
      601 acaaaataaa attgattttt tgtctactcc ttttgatgaa gaaagtgcag actttctaaa
      661 acaaattggt gtacaaggat ttaaaattgg ctcaggagat ttaacaaatt taccttttct
      721 tagaaaaata gatatgtatg ggttgccagt tttattatct acaggtatgg ctgatattca
      781 tgaggtcagt gaggctgtag attgtttcat agatagtcca gttactattt tacattgtac
      841 ttcaaattat ccggctagtt ctgaagatat taatttattg gcgattaaca cattaaaaga
      901 aatgttcggg atacccgttg ggtactccga tcattcattg ggatatgatg ttggtatttg
      961 tgctgttgcg atgggtgcta aggtaattga gaaacatttt accttggata aggaactacc
     1021 tggtccagat cataaagctt ctttagatcc tcaggaattt gcagattttg taaagcatat
     1081 tagaaacact gaaatatttt taggtgatgg tgttaagcga gcaatgcctt ctgaacttag
     1141 tacaaaagaa gtatcaagaa aaagcctagt ggtcactgag aatttaaatg tgggagatat
     1201 tcttacagag aaatctctta caataaaacg tcctggaaat ggaatagctc ctaagttttt
     1261 atatcaatat attggcaaaa aagttaaaag accaataagt gcagatagtg ttctgcttga
     1321 ggaagatgtt gaatgaaaaa aatacttata gtaagtggaa cacgagctga ttatggaata
     1381 tataaacctg ttgctgagaa tttactacaa gataattggg atgtatcatt tgttgtttct
     1441 ggaatgcatt tatcccataa atatggattt actaaagaat tgatcttagc tgacggattt
     1501 agaattagtg gagaagttca aagtttgttt ttagagaaca ataagggaaa catggctcgg
     1561 tctgtggcat tagagataaa tggtttcaca aatatatttg aacaaactag ccctgatttt
     1621 gtattattac taggggatag aggagaaatg ttggcagctg cgatagcttg tttgtatctt
     1681 ggaatcaaaa cagcacatat tcacggaggc gaagtgtctg gtactgtaga tgaatcaata
     1741 agacatgcca taacgaaaat agcatctgtt cattttgctg ctacggaaaa aagcaaagag
     1801 cgtttaataa aaatggggga agattcttgg agagtttttc ccgtcggagc tcctagaata
     1861 gatact
//
LOCUS       DAJSMI010000260         1844 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJSMI010000260 DAJSMI010000000
VERSION     DAJSMI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.265"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..612)
                     /locus_tag="ON430_002751"
     CDS             complement(<1..612)
                     /locus_tag="ON430_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220623.1"
                     /translation="MTETKFIQDPWSKMTTRNGLRGDEAISALQKSIRRGKEKEACEF
                     AYEMYITSPQFEEKLWRRLLAISVEDVGMGNPMAAIMINNLNAMRKEFQYNESDRAMY
                     FFHAIRYLCQSEKDRSSDLLKNIVIKSFAMGYIPEIPDIALDKHTVRGQAMGRGSEHF
                     LHEASKVIPQAKVDNDYKEQYEKIIETYDPDHVVPSTFSFNPWQ"
     gene            653..1345
                     /locus_tag="ON430_002752"
     CDS             653..1345
                     /locus_tag="ON430_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3220624.1"
                     /translation="MDVEMLIDKYQLNDSEAEVLRFMKKNRQSLKNIGIRDVAKASYV
                     STATIINMAKKIGYSGYSELVFALGNSQSDITMPSTFSNEEKEAFIHLMSAYRDKRIM
                     ILGSGFSQNLANYFSESLNLYGFRATANSHLEFLRQQVQQDILLFIISNSGDTVRLAE
                     LAKIANDHFIDVIAFVGQKNSKIGTLATLTISTETYIPQKISDYQPNLFFGTTLNQFE
                     LLLSAALKAIYK"
BASE COUNT          563 a          346 c          330 g          605 t
ORIGIN      
        1 ttgccaagga ttaaatgaaa acgtacttgg cacgacgtga tctggatcat aggtctcgat
       61 gattttttcg tattgttcct tgtagtcatt atcaactttt gcttggggga tcactttact
      121 tgcttcatgc aaaaagtgtt ctgaaccacg ccccattgcc tgtccccgaa cagtatgttt
      181 atccaacgca atatcaggaa tttctggaat atatcccata gcgaaactct tgataacaat
      241 gttcttaagt aaatccgatg aacgatcttt ttcagattgg cataggtaac gaatcgcgtg
      301 gaaaaaatac atcgcacgat cggattcatt gtattggaat tctttgcgca tggcgtttaa
      361 gttattgatc atgatggcgg ccataggatt gcccatgccc acatcctcaa cactgatcgc
      421 caatagcctt ctccagagtt tctcttcaaa ttgtggtgag gtgatataca tttcataagc
      481 aaattcacat gcttcttttt cttttcctcg acgaatcgat ttctgtaatg ctgagatagc
      541 ttcatctcca cgtaagccgt tacgggtagt catttttgac cacggatctt gaataaattt
      601 cgtttctgtc ataatataat actcctatta gataaaattt gaggtgaatc ttatggatgt
      661 tgaaatgctt atagataaat accaactgaa tgactcagaa gctgaagtct tacgatttat
      721 gaaaaaaaat cgtcagtctt taaaaaatat tgggattcgt gacgttgcca aagcatcgta
      781 tgtttctact gcaacgatta tcaatatggc gaagaaaatt ggatattccg gttatagtga
      841 gttggtattt gctctaggaa acagccaatc tgacattacg atgccaagca cattctccaa
      901 tgaggagaag gaagcattca tacatttaat gtctgcgtat cgcgataaac ggattatgat
      961 tcttggctca ggtttttcgc aaaatctagc caattatttt tcggaatctc ttaatctata
     1021 tggatttcgt gcgacggcca atagtcattt agaatttctt agacagcaag tgcaacaaga
     1081 tattctgctt tttattattt ccaattctgg tgatacagtt cgattagccg aattagctaa
     1141 aattgcgaac gatcacttta tcgatgtgat cgcatttgtt gggcaaaaaa attcaaaaat
     1201 cggtacgctg gcaacattaa ccattagcac ggaaacctat attccacaaa aaatatctga
     1261 ttaccaacca aatcttttct ttggcacgac actgaatcaa tttgagcttt tgttgagcgc
     1321 ggcgctgaag gctatttata aatagaagaa cgctgtggcc actgtttctt caaaatctag
     1381 tataaagaat cttctttaga atgtaattgt tttctgtgtg ttctgaacat gtttaggaat
     1441 ttccaaactt gtttagaaaa ctttctcatt ccctccgctg tagaattgaa ctcacataaa
     1501 aaacatctaa accaaaatat ctacaagctt ggaaaggata ataggttgtt attaaaaata
     1561 atttttattc ctgaacctga ataattcata gctttaattc tttggcacat tttattgaaa
     1621 tatgtatcac taatatccct atcaactgat tttgactaaa aagttacttc aaacattcac
     1681 tgttcaaaaa tattgaggaa taaattttgg gtgtaaggca ttttgagcat acttctccct
     1741 ggataacttc ccagtaaaaa aatcgttaga ttgttggatt agatccattg actgcctcgc
     1801 ctcaggcgag caaagaccct gtagaattca ttctgataca catg
//
LOCUS       DAJSMI010000261         1842 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJSMI010000261 DAJSMI010000000
VERSION     DAJSMI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.266"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            79..561
                     /locus_tag="ON430_002753"
     CDS             79..561
                     /locus_tag="ON430_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220625.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            758..1648
                     /locus_tag="ON430_002754"
     CDS             758..1648
                     /locus_tag="ON430_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HCR3220626.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          611 a          313 c          381 g          537 t
ORIGIN      
        1 aaaagttttt ccccctaatt tttttagata gaaaattgta tagtatgatg agagaaaaag
       61 tagtttgggg gaacgttaat gagaagaaaa gcagtaatcg atcggcttta tttatttttt
      121 tggttttttc ctatcgtttt gctacctttt acatgggtaa atgctgttgt acaaggtgcg
      181 gagatattag gcctgatcca aggaagcgga tttgtggttt ctaggaattt tccagctttg
      241 attgttttct atttagtgat aagtgtaggt ctcttattac caaaatctct gatcaatcac
      301 tacaattggc agctgcttta cttttcttgc ttattagtat ttacatctat gttttctttg
      361 ctgcctcgct tgattatcgg ccctaatggt ttgagcgaga gtggattgaa ggatttagct
      421 tattcctcca tgtatttctt gacgatcaaa ccatggttct atttagtaat tgcctcattt
      481 attcttgcaa cattttttta cttctggaca gaaatcagga taaaaagtga acgatctgta
      541 tctataaaaa ataactattg atggtggatc atcagtaaaa aaacatatga gctgcgtaaa
      601 atgcatgttt ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa acgtttacat
      661 gatttttaca taacttaact atatttttac agcaaccata cgagggattt acagtcgact
      721 gataaacttt agttgtaaat aagaaaggag caataaaatg aaaaaagtat tgtttactct
      781 aggtatgatg ggaattcttt tagccggatg cggctcagga tcaggaaata cagatggctc
      841 tgccacacaa aataattcaa gtgactcaaa tgaacaagta aaaatcgttg cggtaggttc
      901 gacagcatta cagccgttag tcgatgcagc acaagaaagc tttgtacaag aaaatccaaa
      961 ttatcaaatc tctgtacaag gaggaggaag cggaactggt ttgagccaag tcgaagctgg
     1021 agcagttact atcggaaact ctgatgtatt tgcagaagaa agagacggag ttgatgcttc
     1081 aaaattagtg gatcataaag tagcagtggt cgggatggca ccaatcgtca ataaagatac
     1141 agatgtcaaa gatatcacca aacaggaatt gattgatatc tttacaggaa aaatcactaa
     1201 ctggaaggaa gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg caaacggcag
     1261 cggtacccgc gcgacattcg aaaaatgggg actggatggt gctacccctg tgcagtccca
     1321 agaacaagat tcatcaggaa cagttcggca gctggtaagt caaacaccag gagcaatcag
     1381 ctatttagct ttctcatatc tcgatgattc cacacaagca ctaagtatcg acggtgtaga
     1441 accaaaagaa gagaatgtag cagataacag ctggggaatc tggtcatatg aacatatgta
     1501 cacgaacgga aaaccttccc ctgaagtcca aaaattctta gactacatga tgacagaaga
     1561 aatccaagaa ggacctgtca aagaattggg ctatttacca atcacaatga tggaagtaga
     1621 acgtgatcac gaaggaaata tcaaataact agcagataaa catgccacgg tagtcgttac
     1681 tattaaggaa aaatcaacta tgtttcccca catatacatt tttcctttaa ttgtgaaaaa
     1741 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg
     1801 tttattcgaa aaaaagagct acgacataga actgtcgtaa ct
//
LOCUS       DAJSMI010000262         1834 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJSMI010000262 DAJSMI010000000
VERSION     DAJSMI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.267"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            41..235
                     /locus_tag="ON430_002755"
     CDS             41..235
                     /locus_tag="ON430_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220627.1"
                     /translation="MSKLLRELTDKEIQSSLSNGGSTCSMYKALLPGHIAACAASAYF
                     YGSSGCTAMRYVSEYIRKNC"
     gene            314..1459
                     /locus_tag="ON430_002756"
     CDS             314..1459
                     /locus_tag="ON430_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3220628.1"
                     /translation="MLRNFSVIIICKNEERVIKRCIESIQKNLKKNDEIIVVDTGSTD
                     NTIKIISSIENVKLFNYEWKDDFADARNFGISKAKKDWIFFIDSDEIMSKGCLEQLNA
                     SINEAMAYSENNEKLVFSPKAVNTDDSVFYNAGRIFANDGNIKYEGCVHEYPVVTDKS
                     LTLVSLKLPRVIVRHDGYEKEVRADKNKTVRNITLIKKILQEEPNNSQYYYYYYRDSK
                     PLITIEEYEQGMLNFFEKFPDSPYIDQVVRDLAYHYIQVGKNELAEEYINMLFESAEN
                     GAKENYHLAILYTGMNEMGKIKAQQKDILKLLVYAHDNVLQQEEELFDKGYIFDDLIS
                     ILFFQIEEYETAYKICEKLDSCGYASNISKMISKLKVVLSPNSKNFR"
     gene            1551..>1834
                     /locus_tag="ON430_002757"
     CDS             1551..>1834
                     /locus_tag="ON430_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCR3220629.1"
                     /translation="MKYKVIPQIGEEDCGAACVAMIVENYFKKKISICEIRPLIKNTK
                     MGTSFFDLKRGLKRLGVDCKISQAVKQKDVFEEITYPFITQIYNETGIHY"
BASE COUNT          723 a          209 c          325 g          577 t
ORIGIN      
        1 gtagttaaac aactagaaaa ttattttgga ggagatattt atgagtaaat tattacgtga
       61 attaacagac aaggaaatac aatcttcgtt atctaatggt ggatctacat gcagtatgta
      121 taaagcgcta ttacctggac atattgctgc atgtgcagct agtgcatatt tttatggttc
      181 aagtggctgt accgcaatga gatatgtttc agaatatata agaaagaatt gttaaccatt
      241 caattttttt aatgtagttt atatagcatg ttaatttgtt gtttaactat cttattctta
      301 ggaggatata tctatgctta gaaatttttc agtaataatt atatgtaaaa atgaagaaag
      361 agtaatcaag cgatgcatag aatctataca aaaaaatttg aaaaagaatg atgaaatcat
      421 agtagtggat accgggtcta cagataatac tataaaaatt atatctagta tagaaaatgt
      481 aaaactattc aattatgaat ggaaagatga ctttgcagat gcaagaaact ttggtatttc
      541 aaaggcaaaa aaagattgga ttttttttat tgattccgat gagataatgt caaaaggatg
      601 tttagagcaa ttaaatgcta gtataaatga agcaatggcc tactctgaaa ataatgaaaa
      661 gcttgttttt tcgcctaaag cagttaatac ggatgattct gtattctaca atgcaggaag
      721 aatatttgct aatgatggaa acattaaata cgaagggtgt gtacacgaat atcctgtcgt
      781 gactgataaa agtttaacgc ttgtttcttt aaaacttcct agagttatag taagacatga
      841 tggttatgaa aaagaggtac gagcagacaa aaataaaaca gtaagaaata taactttaat
      901 taaaaaaatc cttcaggaag aacctaacaa ctctcagtat tattattatt actacagaga
      961 ttcaaaaccc ttgattacga ttgaagaata cgaacaaggg atgctaaatt tttttgaaaa
     1021 atttcccgat agtccttaca ttgatcaagt agtaagagat ctagcttatc attatataca
     1081 agttggtaaa aatgaattag cagaggaata tattaatatg ttatttgaga gcgcagaaaa
     1141 tggagcgaaa gaaaattatc atttagcaat attatacaca gggatgaatg aaatgggtaa
     1201 gataaaagcc caacaaaaag acatattaaa gttacttgta tatgcacatg ataatgtttt
     1261 acagcaagag gaagagttgt ttgataaggg atatatattc gatgatttaa taagtatact
     1321 gttttttcag attgaggaat acgaaaccgc atataaaata tgtgaaaaac ttgattcttg
     1381 tggctatgct tctaacattt caaaaatgat tagtaaactt aaagtggttc taagtcctaa
     1441 ttctaaaaat tttaggtaaa aggagtcaat tcttagatac cataacttaa ttttattata
     1501 agtaaatatc aacttaaatt aataatgctt tttagataag gaagataaaa atgaaatata
     1561 aagttatacc acaaataggt gaagaagatt gcggtgctgc atgtgtagca atgatagtag
     1621 aaaactattt caaaaaaaaa atatcaattt gtgaaataag accgttaatt aagaatacaa
     1681 aaatggggac ttctttcttt gacttaaaaa gaggactaaa gcgtttggga gttgattgca
     1741 aaatttcgca ggcagtaaag cagaaagacg tttttgaaga aattacatac ccatttatta
     1801 cacaaattta caatgagaca ggtattcatt acat
//
LOCUS       DAJSMI010000263         1827 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJSMI010000263 DAJSMI010000000
VERSION     DAJSMI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.268"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(121..204)
                     /locus_tag="ON430_002758"
     tRNA            complement(121..204)
                     /locus_tag="ON430_002758"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Leu,seq:cag)
     gene            732..1232
                     /locus_tag="ON430_002759"
     CDS             732..1232
                     /locus_tag="ON430_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3220630.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1422..1766)
                     /locus_tag="ON430_002760"
     CDS             complement(1422..1766)
                     /locus_tag="ON430_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCR3220631.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          561 a          332 c          292 g          642 t
ORIGIN      
        1 tttttgattt atttgaatct ggttccgttg tttaaaattt ttcctatatt cttttagata
       61 atatgttatt ttctttctca tcgctccagg tgtaccttct ctttataaaa aaacaaatta
      121 tgcggctaag aagactcgaa cttccacgag gtcaccctca ctagcgcctg aagctagcgc
      181 gtctgccaat tccgccatag ccgcatcaca tcttttagtt taggattaca tgtgtttttt
      241 gtcaagaaca tatttttgtt ctttttcatt tctgtacgcc ctcccttatg ccatctacac
      301 aaataattca tccgagcaac tccatcatct attgagggtt ggtgaaaaaa tctgttttat
      361 ccatatgatc gatttcaatg ttaaacgaac gactctggga ttagtaacta ttttattttt
      421 ttcttcattt cttttttcct ctttttctcg attcagctat cttctttcat ttggatattg
      481 attatttctt ctgttgatta ctatccttct ggaattctag acgctgaagt aattcacgtt
      541 ttctttcttc tagagatcat aagtaacgaa tgaacgaact tcaaataatt tttttattgt
      601 aattcatatt tgtattgttc gatgattctt aatacgatat gacacaataa ataaaaatag
      661 gcatggctat ttacaacaat agtgctatag tagaaaacaa atgaataagg ttacttaaag
      721 aggtgaatac gatgaaactg atcaaactat gggaagcaga tttaaagaaa gcttatcagc
      781 tacaaactag ctttggacca aatgagagtg gatttatcaa caatgcttat ggctatacat
      841 ttgctgaatt tttagattat gtaaaaaaat gcgaaaaaac gtctttagga atcgatcttc
      901 ctgagagtta cgttcccgca accgtcttta ttttagaaag tgatgaacat gagtatgtag
      961 gaatttttaa tttgcgccat aaactgaacg aaacattaag aaacggcgct ggccatattg
     1021 gttacggcat ctcccctaat tatcgtaaaa aaggatatgc cacacaagga ttagcattag
     1081 tactacaaga ggcaaaaaaa atcggcattg atgaggcact tttgtctgta aataaaaaaa
     1141 atcttggcag tcttaaagca caacaaaaaa atggggctgc gatttatcgt gaagatgaga
     1201 aagaatacta tacgaaaatc aaactgatgt aaaaaagaag gattccaaaa ggaaagggat
     1261 ttgaatgtcc tttatcaata gaacacattt atttaattaa cttataatat agcagacaaa
     1321 attttataaa caaagaaacc ttttatctct tttcttctag aaaggataaa aggctttttt
     1381 gtttttccta tttgcttctc atagctagtc agccaagatt tctatcgtat ccccaacgca
     1441 atccgtgcat aacggctcat cttatctgtc gtccatgctg gataccagac tagtttgact
     1501 tctgctttcg ttacttctgg cacttctttt aatgcgtcat ggatagattc tgttaaaata
     1561 tctgctaatg gacagcccat agttgttaga gtcatcttaa tgactgtttc tccattttca
     1621 ggattgaatt ctatatcata aatcaagcct aaattgacga tgtcgattcc taattcagga
     1681 tcaatcacca tttccaaggc agcaagaata cgttctttga ttagttctat ttcttgtcca
     1741 gaccgttctt ggtttgcttc gctcatcgta cgacactcct taacacgatt tttatttatc
     1801 atactctttt tttagctgct tgtaaat
//
LOCUS       DAJSMI010000264         1783 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJSMI010000264 DAJSMI010000000
VERSION     DAJSMI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.269"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..294
                     /locus_tag="ON430_002761"
     CDS             <1..294
                     /locus_tag="ON430_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HCR3220632.1"
                     /translation="TSVTYLIQCGFTEKMAIEALYPLIEFNIRNIKEKGIIESLTGPV
                     ERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDYSELEKYLGES"
     gene            306..1133
                     /gene="panB"
                     /locus_tag="ON430_002762"
     CDS             306..1133
                     /gene="panB"
                     /locus_tag="ON430_002762"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR3220633.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1148..>1783
                     /locus_tag="ON430_002763"
     CDS             1148..>1783
                     /locus_tag="ON430_002763"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR3220634.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEA"
BASE COUNT          693 a          185 c          383 g          522 t
ORIGIN      
        1 acaagcgtta catatctaat tcaatgtgga tttactgaaa aaatggcaat tgaagcttta
       61 tatccattaa ttgaatttaa tattagaaat ataaaagaaa aaggaattat agaaagtttg
      121 acagggccag ttgaacgttg tgatatttca acaattaaag gtcactgtga agttttatca
      181 gatgaagaca gagctttata tatgctatta tcaaaaaatg tattagagat agctatgtta
      241 aaaaatatta atagagatta tagtgaatta gaaaagtatt taggagagag ttaggagaga
      301 aatagatgaa aaatacagca gttacattta aagaatctaa attaagaaat gaaaaattaa
      361 caatgcttac agcttatgac tattcaacag caaaaataat tgatgaagct gggataaatg
      421 gaatattagt aggagattct cttggtatgg tatgtctagg acatgaagat acactttcag
      481 taacaatgga agacatgatt catcatacta gagcagttac aagaggagct aagaacactt
      541 tagttgttgc agatatgcca tttatgtctt atcaaacttc agtatatgat tcagtagtta
      601 atgcaggaag attaataaaa gaaggacgag ctcaggttgt taaattagaa ggagggatag
      661 aagtatgcga taaaatagag gctatagtaa aggcttctat acctgttatg gcgcatatag
      721 gtttaacacc tcaatctgtt aatgcatttg gaggatttaa ggtacaagga aaggacaagg
      781 aagctgctaa agaattaata agagctgcta aagctgtaga aaaagcagga gcattcgctg
      841 tagtgttaga atgtgttcca actaagcttg cagagttaat ttctaaagag atatcaatac
      901 ctacaattgg tattggagca ggtgcagggt gtgatggaca aattcttgta tatcaagata
      961 tgttggggat gtatagtgat tttaccccta aatttgtgaa gaaatatgct aatttatcag
     1021 aagagatgaa taaagcattt acaaaatata tagaggaagt aaaagatgga gtttttccag
     1081 gtccagaaca tgggtttaaa ataagtgatg atgttttaga aaaattatat taaaaggggc
     1141 aagaaatatg gttatattta aaacagtaaa tgatgttaga agtcaagtaa aagagtggaa
     1201 aaaacaaggc ttaaaggtag gattagttcc tactatggga tatttacatg aaggtcatga
     1261 aagtttaatt agaaaagcat caaaagaaaa tgataaagtt gttgtaagta tctttgtaaa
     1321 tccaactcag tttgggaaaa atgaagattt aggtagttat ccaagagact tagaaagaga
     1381 tatagaagta tgcacaagag gtagagcaac agcaatattt aatcctgaag ttgaagagat
     1441 gtattgtgac aatgcaagta cttttgttaa tataactgga ttaacagaag gtttatgtgg
     1501 agctagtagg ccaatacatt ttagaggggt ttgcactgtt gtaagtaaat tatttaacat
     1561 aataccagca gatagagctt attttggaga aaaggatgca caacagttag ctgtaattaa
     1621 aagaatggtt agggacctta atattgatat agacgttgta gggtgtccaa ttataaggga
     1681 agaggatgga ctagctaaaa gttctagaaa cacatactta agtttagagg aaagatcttc
     1741 agctactatt ttaaataaat ctttaacttt agctaaggaa gct
//
LOCUS       DAJSMI010000265         1717 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJSMI010000265 DAJSMI010000000
VERSION     DAJSMI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.270"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            148..744
                     /locus_tag="ON430_002764"
     CDS             148..744
                     /locus_tag="ON430_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3220635.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(802..1284)
                     /locus_tag="ON430_002765"
     CDS             complement(802..1284)
                     /locus_tag="ON430_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220636.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1390..1587)
                     /locus_tag="ON430_002766"
     CDS             complement(1390..1587)
                     /locus_tag="ON430_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220637.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          567 a          348 c          266 g          536 t
ORIGIN      
        1 ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg tgagcatatc
       61 tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa aaaaatgcaa
      121 aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt gaaaaaccgc
      181 cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt aacaactttg
      241 atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac acgcgcagtt
      301 atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc acttcgccca
      361 ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt caaaaatggt
      421 tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca agaacaattt
      481 gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat cgcaacagct
      541 aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca ttacaaccca
      601 gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa actacgttcg
      661 caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat gaacgatgcc
      721 gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt tgtgacaaac
      781 atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct ctgcctcatc
      841 gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa atttcagcat
      901 cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac cttttggctg
      961 atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa agctattcaa
     1021 aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa aaatcattgc
     1081 tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg tataccaaat
     1141 gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca cagtccgata
     1201 aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga ataaaaacaa
     1261 tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc cagaaaatag
     1321 gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc gcacgtccaa
     1381 ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt tttcatctaa
     1441 taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt ttgtcatttt
     1501 tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg catattgttc
     1561 tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga acttttctcc
     1621 acattaccac tgctatttct ttggagattt aactcattga tttttattta cagttaaaaa
     1681 tcaaccttac aggcattata gcgtactgtt ttcgttt
//
LOCUS       DAJSMI010000266         1708 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJSMI010000266 DAJSMI010000000
VERSION     DAJSMI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.271"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            86..385
                     /locus_tag="ON430_002767"
     CDS             86..385
                     /locus_tag="ON430_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220638.1"
                     /translation="MNGQTPQTPQTPVVEESTQQSQQTETTTEQVKLYGEVLTDPHEK
                     AEMIRDLYEGNQGFNRDILNSIPDQEIIDSFVGNATNAQLAQSTTNLLEKYPNLK"
     gene            987..1592
                     /locus_tag="ON430_002768"
     CDS             987..1592
                     /locus_tag="ON430_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCR3220639.1"
                     /translation="MVLENKLGITNQIALAKAEERISKQKAKHLYDSGKINEIEVGTF
                     KGLATIHFYLFSDIYKFAGEIRTVNLAKGNFRFAPLMYLETSLQYIDKMPQSTYKEII
                     EKYVEMNIAHPFREGNGRSMRIWLDLMLKKELQKVIDWNLVDKEDYLSAMERSPINDL
                     EISHLLSQALTEQIHDRQLFMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          671 a          210 c          299 g          528 t
ORIGIN      
        1 gcaaaaaaat cagggtaatg aaaataatta tctgtacttt aacgaaactc aaaatcaaca
       61 gttaaaacaa ggtttcattg atattgtgaa tggtcaaact cctcaaactc ctcaaactcc
      121 agtagtagaa gaatctactc aacagtctca acaaacagaa accactactg aacaagtaaa
      181 attatatgga gaagttctta ctgatccaca tgaaaaggca gaaatgatta gagacctata
      241 tgagggtaat caaggattta atagagatat tttgaattca attccagatc aagaaatcat
      301 tgattcattt gtaggtaatg ctacgaatgc acaacttgca caaagtacaa cgaatctatt
      361 agaaaaatac cctaatttga aataagagtt acaaatggac gaggctttta ggatgattca
      421 tcattctaac atggttcaat aattagagtt atacatcatc aagtaataac tgattcttaa
      481 aaagcaccat aagcttttta agaatcagtt ttatctataa agagggagaa agaatgtatt
      541 aaatgacttt ttaactaata tacataaaaa caataatagg aatgagaatt gaaagagcac
      601 aagcattctt ttgaatgaga tatttaattg aaaaccaata cagtactaga agtacccaaa
      661 gtgaccaaaa gatagttgat aaaaaatgag taagaaaagt aaaatgagat aaaaaattgt
      721 cactttaggt actttggtac taagaaaggc ttgtttttgt ctatttgttt cactaaagta
      781 tataaaacag aattaatgca ggaatcatag aaagtttgtc atactttcta tgattccctg
      841 ttttacttga ctttttgata taatagaata gttaagaaag tattcaagag aatattttag
      901 aaaatagagt ttatgagttt atgcactgta gaaagaattt gagcgtcaat gatttagtga
      961 tttaatatat gaatgaggtg attctcttgg tattggaaaa taaattagga ataaccaatc
     1021 aaatagctct tgcgaaggca gaggaacgaa taagtaaaca aaaagcaaaa catttatatg
     1081 atagtggaaa aataaatgaa atagaggttg gaacatttaa gggcttagca acgattcatt
     1141 tttatttgtt ttcagatatt tataaatttg caggagaaat tcgaacagtg aatttagcga
     1201 aggggaattt tcgttttgcc ccattaatgt acttagaaac ttctttacag tatattgata
     1261 aaatgcctca aagtacgtac aaagaaatta ttgagaaata tgttgaaatg aatattgccc
     1321 atccttttcg agaaggaaat gggcgaagta tgcggatttg gttggatctt atgttaaaga
     1381 aagaactaca aaaggtaatt gattggaatt tagtagataa ggaagactat ttatctgcta
     1441 tggaacgtag tccaatcaac gatttagaaa ttagtcattt actttctcaa gcactaacag
     1501 aacaaatcca tgatcgtcaa ctatttatga aaggaattga tgtaagttat ttttatgagg
     1561 ggtattcaga gtatacgatt gatgatctat aaatctgttt aggtttaatg tatttctaca
     1621 caattattct tacaataaga aaagagctaa catagagact catagaaagt atgaaggact
     1681 ttctatgagt ctttgttttt caaaatag
//
LOCUS       DAJSMI010000267         1704 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJSMI010000267 DAJSMI010000000
VERSION     DAJSMI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.272"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            245..859
                     /locus_tag="ON430_002769"
     CDS             245..859
                     /locus_tag="ON430_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3220640.1"
                     /translation="MRVAYIRVSSIDQNEQRQVEEMKKFGAERIFIEKQSGATITHRP
                     VFQEVLDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSTVNHE"
     gene            complement(883..1233)
                     /locus_tag="ON430_002770"
     CDS             complement(883..1233)
                     /locus_tag="ON430_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3220641.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1227..1490)
                     /locus_tag="ON430_002771"
     CDS             complement(1227..1490)
                     /locus_tag="ON430_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HCR3220642.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          576 a          295 c          261 g          572 t
ORIGIN      
        1 atttaattgg cacagttttt tctaattatt tgtcatattc atagtcaact atagctacaa
       61 aagcaactaa ttttggagat agattgagat atttaaaaat ctatcatgaa aaattagttg
      121 cttttgtcac ttagcgctga aaatttatgc catttatcta gaaaaatgac aaacattgaa
      181 aaatctggtt ttctcatgta tgattagggt gtgttttaaa tagttgcaga aagtaggtaa
      241 aaatttgaga gttgcataca tacgtgtctc atcgatcgat caaaatgaac aacgccaagt
      301 tgaagaaatg aaaaaattcg gtgcagagag aattttcatc gagaaacaat ctggcgctac
      361 aattactcat cgacctgttt tccaagaagt attggattac gtccgagatc aagatatttt
      421 tattgtggaa gctattgatc gacttggaag aaattacgac gagatcatcg cttcagtcaa
      481 ttacttgaag aagaaaaatg tgcagctgat cattacaagt ctaccgatca tggccgaagc
      541 aattggcaat cctctcctgg ataaattcat taaagatttg atcattcaaa ttttggccat
      601 gatcgctgaa caagaacgta ccgaatctaa acgacgtcag gcacaaggca ttaagattgc
      661 gaaagcgaac ggtgtttata aaggacgtcc taagctatac agtgccaatg caaaagatcc
      721 ccaacgtcgt cttgtttata aaaacatcgt tgaggatcta aaaaaaggtg ttgcgatcgc
      781 taagattgca aaagactaca atgttacgag acaaaccgta tatagaataa aaaaagacag
      841 cacggtcaat cacgaatagt tgatcatgct gctgtttcta ttttatttgc ttactttttg
      901 aaaaactgtt ctaactttca tcaataactc ctcaactaaa ctatcatgcg ctttttccaa
      961 aaaaatacac tctctggctt cataatcaat tgtcgttagt tgatctaata atacttttcc
     1021 tgttgtcttc atttcatatt ctgagtttat atttacatat agcggatagt ttctagttgt
     1081 actagaaatt ggagcaactg taacaacgtt actataatcc gcaataatat catgacttaa
     1141 aacaatataa ggccttttac ctgtctgttc gtgtccagat cgaggtgctg tattcaataa
     1201 tagaatatcg ccttgtctag gttttctcac catttttcat tacctactgc ttcacctaaa
     1261 ttttgaatcg tcacatgatt tctttcatca gtgtaatttt caaataactg ttcaattgtt
     1321 aattcatttt ctatattagc cgcttcgata attagccgct tctgaccgtt tacttcttct
     1381 tgtattatct tcactttgtc ttctgtatga attccaagat attccataat atactttggc
     1441 aatctaaggc cattactatt tccccatttt tttataggta acatttccat agtagacacc
     1501 tctttctgta tatacatagt ataacataca taagaaaaaa cgtatatact taattatcct
     1561 tttctattaa attagattaa cattccatga atccataaac tatgttaaca tttatatgaa
     1621 aatagtcgaa aggaaatcat aatatgttta agaaaacaaa atctttagaa tcaggtaaga
     1681 aaaaaattga tctaaaaata atct
//
LOCUS       DAJSMI010000268         1685 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJSMI010000268 DAJSMI010000000
VERSION     DAJSMI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.273"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            18..569
                     /gene="lepB"
                     /locus_tag="ON430_002772"
     CDS             18..569
                     /gene="lepB"
                     /locus_tag="ON430_002772"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3220643.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(652..1152)
                     /locus_tag="ON430_002773"
     CDS             complement(652..1152)
                     /locus_tag="ON430_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR3220644.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(1431..1685)
                     /locus_tag="ON430_002774"
     CDS             complement(1431..1685)
                     /locus_tag="ON430_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCR3220645.1"
                     /translation="MLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQI
                     AKENGGTFHEVDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT          592 a          291 c          292 g          510 t
ORIGIN      
        1 ggatggattg atagagattg acaaaaaaac aacgctatat taatcgcttc tggcttgtat
       61 tcaaatactt acttgtttct gtattccttg cgttcatgtt gcggggattt ttattcatcc
      121 cggtaccagt ggaaggcaat tcaatggaaa acgtgttgaa gcaaggtgat atggttgtga
      181 tggaaaaatt ttcggagatc agacgatttg atattgttgt ttttcaatta gcagatggca
      241 cgatttatat caaacgagta atcggtcttc caggagaaaa tgtcagctac caaaatgatc
      301 aattgaagat caatgggaaa gtagttaagg aaccttatct aactaaaaac ctgaaatcag
      361 atcatgcaaa tgcatcctat acgacggact ttacactaca agagctaacg ggacaatcca
      421 agcttccgga agataattat tttgtacttg gagataatcg aagagtttca aaagatagtc
      481 gttcgtttgg cacgatcaat aaaactgata ttctaggtaa agcaagattt gtctattatc
      541 cacttgatga aataaaatgg atccagtgag aaacaataaa gcgtgcagtc tgttgagact
      601 gcacgcttta ttgtttaatc caaaatcagt ttactacctt agtacgtaaa atcaaacagc
      661 tcgtttaaac acttttctag cctctaaagt tttgataaat gaataaacaa ccattatttg
      721 aatagggatt tggatcatat tcctgatgaa tctacctaga aaaattactt tgaaatctgc
      781 accaaacaaa acagacatca gccaagaatt gataaaaagg ctgactggga tcataatcaa
      841 taattgggca gcgatgacgg aaggcaggtt tatcttttta tgatataaaa ataagccatt
      901 aataaatcca ccaaggaaag ccgtgataag aaaagcagct tgaaacgttc ctgaaccaaa
      961 tatcgtgaaa tttaaagtgt tcgacaacat ttcaagcaaa ccagcccaga tcggtccata
     1021 tagataaccc ataagcgcca tacttaaaaa tccgaatgtg atttgaaaat aagcaggacc
     1081 aatcgctatc ttgtataaga tgacatcaag ggctaataaa agagcagagg tcgacaacaa
     1141 tcttgcattc atttcgggca atctaaaaac gtcttttgca atcaaagtgt tcaaaatatt
     1201 ctctcctttg tcagttaata ataaaaggat accataaaat caccaatgtg tcatgacaca
     1261 ttggtgattt acatacactg gcatctaaca attaaaaaaa gggtgagaag actgctttta
     1321 taaactgtaa aaaagagatt gtacgaataa aaaaaataga ggcggagaat acttatcgta
     1381 tcatccgcct ttgttttttt gttataaaaa acgtcagatt agttatctgc ttacatcttc
     1441 atccatacgg gttctaatgt atttccgtct agatcttgga cttctaatga aaacatttct
     1501 tcttcaggca ttcccatatc tacttcgtgg aaagtaccac cattttcttt tgcaatttga
     1561 gcaaattttt ttacttcatc cgcattttca acactaaacg aaatcaaagc accacttgtt
     1621 tttttattat cagctatttc tttatctttt ataaattttt tataaaaatc atgagagaga
     1681 agcat
//
LOCUS       DAJSMI010000269         1669 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJSMI010000269 DAJSMI010000000
VERSION     DAJSMI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.274"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            21..443
                     /locus_tag="ON430_002775"
     CDS             21..443
                     /locus_tag="ON430_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220646.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            463..>1669
                     /locus_tag="ON430_002776"
     CDS             463..>1669
                     /locus_tag="ON430_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220647.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDQEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYVVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYRNLPEKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAK"
BASE COUNT          682 a          253 c          328 g          406 t
ORIGIN      
        1 ataaaaggag gaaatcaatt ttggcaaaag caacaagaaa acatgtatat ctttacgata
       61 tgcatatcga agtacttgat gaaataattt ctactcatga agatatatct aattatagtg
      121 aagcagtcag acatttgtgt atgatgtatg atccaaaaat cgaagaaaaa ataaatccaa
      181 ttcaaattaa attgaatgca atgggaaaag aaatttctat tttaaccgag atggtttcag
      241 agtttgcgga tcttcatttg aaagatacag ggattttaat tggtacaaat tcaactgttt
      301 accaagaagc aaaagagcgt gtggaaggga aaataaaagc gaatcaaacg aagaagtatt
      361 ctcctaaaat caagaaatca ataaatgaat ctgttcatgc cagagtagac ccgtttgatc
      421 caacaaagtt tttaaaaaga taaaaagtag aaggggaaca tgatgaataa atcgcctgca
      481 gtcattctca cttgtcaatt taccttgccc aataaaaaat ctttttcaac atacattgat
      541 tatatgacaa gagaaaaagc gctggaagaa atggaacgac gtacgcctga agaagaacag
      601 gaactgcaac gaatcaagaa tgctttagag gatttttata ttcctgaagg agaaactttt
      661 tcggagagta agacggatca agaattcagc gaaacgtcga atgaagctca atctttgatg
      721 ggaaatggaa tcaactttga caagttagag gaacaagatt ttaccaaata tctttcttat
      781 atgacccgtc actatgcgtt agaacaaaag aaagaactga cgcaaaggga acaaaaagaa
      841 taccaacgct tgaaccaagc aataaaaaaa tatcaaacag agccacacaa aaaagcttca
      901 ggaaaccttc ctggagtttt ttctatggct tcagacgaag tgaaaggaag tgatctgaag
      961 gagattaagc aaatcttcca aagaggacaa caaaaaggct caatcattta tcaagatgtt
     1021 gtctctcatg ataattcgta tcttgaaaaa ataggattgt acgatcccaa aacagatact
     1081 ttagatgaag aagggttaaa agcagctgga agaaaaatga tggagacatt attcgagaaa
     1141 gagcaactga acgaaacagg ctactggatg gcaacgattc accgtaacac taaacatatc
     1201 catattcatt ttgcagttgt tgaaaaagaa aatacgcgta aaccttacac tgaggttgaa
     1261 aatggtgtga gttatgttgt accgaaagga aagagaaggc aagcaacatt agacgcaatg
     1321 aaaaccaatt atgtgcatga gttagaacgt ttctcttatg aaaaggatcg aatgattggt
     1381 atggagaaac gaaatttatt gacacgaaaa agtgatttgc gtaacagctt aacaaaagtg
     1441 gtaaaagagc cagtacgtta tgatcgacac gcaatgaact tattaaatga agtatataga
     1501 aacttacctg aaaaacgttc ggaatggaat tatggggatg aaaatcgaac caaactttca
     1561 ccatacacaa gagagaaact cgacgagtta acccaatatg tgttaacaaa agaacctgaa
     1621 taccaagaat atatacagat ttcaaaagca ttgaaagaag aagcaaaaa
//
LOCUS       DAJSMI010000270         1663 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJSMI010000270 DAJSMI010000000
VERSION     DAJSMI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.275"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            220..>1663
                     /locus_tag="ON430_002777"
     CDS             220..>1663
                     /locus_tag="ON430_002777"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR3220648.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT          563 a          315 c          328 g          457 t
ORIGIN      
        1 gttattcgaa ccttatttta agttacttat tatttaatgt atcgaaaaag gtgaaatagg
       61 tttgttataa taagaattaa gtaagacatg aattgtctaa gcaaatgtag cttatccatt
      121 tcattaaaat acatatggtg aaaaaacaag ttcaaatagt aactaatttt ttaaaaaact
      181 gatttataaa aaatacaaca cgaatgagga aaataaccaa tgacagaaca aatcgaaaaa
      241 aattcaaaaa gcttatatca ggcactgtgg aattccgcag atatcctgcg ttcaaaaatg
      301 gacgctaatg aatacaagag ctatttacta ggcttggtct tttataaata cttatccgac
      361 aacatgctgc ggtatgtctc cgttctattg gaggaagaaa cggacgacct aaataaagca
      421 caagaccttt acgttgaagc atttaaagat gcagatataa aggatgatct cttgtatgaa
      481 ctaaaagatg agttttcata taccatcgca cccgcattga cctttaccgc gcaagtggca
      541 gccattcatg atggatcttt tcaattagaa gacctggtac aaggtttccg tgacattgaa
      601 caatccagcg aaaactttga gaacttattt gaagacattg atctgtattc taaaaaactg
      661 gggtcaaccc cacaaaaaca aaataaaacc atcgcagatg ttatgaaaga attagaaggg
      721 ttaaacatgg cggggcatgc tggcgatgtc ttaggcgatg cctatgagta cttaattggc
      781 caattcgcat ctgaatccgg taaaaaagcc ggtgaattct ataccccaca accggtcgca
      841 aaattgatga cacaaattgt tttacaagga aaagaagata aaaaaggctt ttctgtttac
      901 gacgcgacca tgggttcggg ctcgttactt ttgaacgcta aaaaatattc ccatcaaccg
      961 ggaaccattt cttatttcgg acaagagttg aatacgtcta cctataattt ggcgcgtatg
     1021 aacatgattt tgcatggggt tcccattgcc aaccaacact tgcataatgc ggataccttg
     1081 gatcaagact ggccaacaga agagccaacg aacttcgatg gggtgctcat gaacccaccg
     1141 tattctgcaa aatggtcggc tgataaaggc ttcttagatg acccacgctt ctctgcctat
     1201 ggcgtattag cccctaaatc aaaagcggac tttgctttct tattacatgg ctactatcat
     1261 ttgaaagata cgggcgtcat ggccattgtt ttgccacatg gggtgctctt tagagggggc
     1321 gcagaaggga agatacgcaa agccttgtta gaaaatggtg ccattgacac cgtcattggc
     1381 ttacctgcta acatcttctt taatacgtcc attccaacga ccgtaattat cttgaagaaa
     1441 gaccgtgcga aaaaagatgt gctctttatt gatgcgtcac aagattttga aaaaataaaa
     1501 acgcaaaata cgctacgaga ttaccatatt gacgctattt tagaggccta taaaacccgt
     1561 acaattaaag acaagtatgc ttatgtcgca gaatttgatg aaattgtgga gaatgattac
     1621 aacttaaaca ttccccgtta cgtggatacc tttgaagaag aag
//
LOCUS       DAJSMI010000271         1639 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJSMI010000271 DAJSMI010000000
VERSION     DAJSMI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.276"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(192..1484)
                     /locus_tag="ON430_002778"
     CDS             complement(192..1484)
                     /locus_tag="ON430_002778"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR3220649.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          463 a          353 c          285 g          538 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagagac agcctatcgg caataaggct aaacgtctct ttcactacct cttcaagtta
      121 ttattatcgt atagataaac acttcgactc gctctggtaa gaacgtcctg attcagtcgt
      181 cgtttttatc cttagatttg cgcccagacg ctttctaaga tgtttgtttg tgttctatca
      241 ggtccgacag aaaatgttga gatacgtaca ccaacaagct ctgagatacg acgtacatag
      301 ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc ctgtaatgtc ttctgaccag
      361 cctggcaatt cttcatagat tggtttgcag cggttcaact ctttcaagct tgctggatag
      421 tggtagatta gttcgccatc taattcataa gccgtacaaa tttttactgt ttccaatcca
      481 cttaatacat cgatcgagtt taaagatagg ttggtaatac ctgaaactcg tttagaatgg
      541 cgcataacta cagaatcaaa ccagcctaca cgacgcggac gaccagttgt cgtaccgtat
      601 tctcgtccta cttcgcggat ttgttctcct gtttcatcaa ataattcagt tggaaatggt
      661 ccatcaccga cacgtgacgt ataggctttg cagacgccga caactttatt gatttttgaa
      721 ggccctacac cgcttccgat tgttacccca ccggctactg ggttagaaga agtaacaaaa
      781 ggataagttc cctggtcgat atccagcatt acaccttgtg cgccttcaaa caacacgcgt
      841 ttgcctgcat ccaaagcatc attcaagata acggaagtat ctgtgacgta ttgtttgatt
      901 tgttgtccgt attcataata ttcttcaaaa atatcgtcaa aatcaagtgg ttctgaatca
      961 aacattttta caaattgacg gtttttgtca tctagattca aacgaagacg ttcttcaaaa
     1021 atttctttat ctaataagtc tgcaatacgg atacctacac gagctgcttt atccatatag
     1081 gcaggtccga ttcctttaat cgttgtacca atcttatttt cgcccttagc atcttcttgt
     1141 aattggtcca atttgatatg ataaggcaag attacgtgcg cgcggtcaga aatacgcaaa
     1201 ttacttgttt ctacaccttt ttcatgtaaa taagctaatt ctttaaccaa tgatttagga
     1261 ttgactacaa cgccgtttcc gatcacactg atcttgtctt tataaaaaat cccagaagga
     1321 atcaaatgca gtttataagt cacaccgtta aacttaattg tgtgccctgc attgtcgcca
     1381 ccttggtacc gtgcaatcac ttcagcattt tcactgagaa agtctgtgat ttttcctttc
     1441 ccttcatcgc cccattgcgt acctactact actacagatg acatgtgaac acctcgttct
     1501 atattattta atcctgaacc attgtatcaa taatagatac atatttcaaa taaaatcgaa
     1561 tgatttttat ttttatttta taaaaacgct taaaaaacga actttatctt tttagaatgg
     1621 ttattaaaaa agaggttgt
//
LOCUS       DAJSMI010000272         1633 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJSMI010000272 DAJSMI010000000
VERSION     DAJSMI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.277"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            188..429
                     /locus_tag="ON430_002779"
                     /pseudo
     CDS             188..429
                     /locus_tag="ON430_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            478..561
                     /locus_tag="ON430_002780"
     CDS             478..561
                     /locus_tag="ON430_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HCR3220650.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            686..1423
                     /gene="erm(B)"
                     /locus_tag="ON430_002781"
     CDS             686..1423
                     /gene="erm(B)"
                     /locus_tag="ON430_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR3220651.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          623 a          251 c          284 g          475 t
ORIGIN      
        1 gttgtaaaaa ttggggaaaa gggatatatt gattttatat ctttctcaaa ataaacataa
       61 aagcccttta aacagggctt ttatatatta atcacttatc acaaatcaca aatcacttat
      121 cacaaatcac ttgtgatttg tgattgttga tgataaaata agaataagaa gaaatagaaa
      181 gaagtgagtg attgtgggaa atttaggcgc acaaaaagaa aaacgaaatg atacaccaat
      241 cagtgcaaaa aaagatataa tgggagataa gacggttcgt gttcgtgctg acttgcacca
      301 tatcataaaa atcgaaacag caaagaatgg cggaaacgta aaagaagtta tggaaataag
      361 acttagaagc aaacttaaga gtgtgttgat agtgcagtat cttaaaattt tgtgtataat
      421 aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg
      481 ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca gactaaaaac
      541 agtgattacg cagataaata aatacgttag attaattcct accagtgact aatcttatga
      601 ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat ttatttacag
      661 atgtaatcac ttcaggagtg attacatgaa caaaaatata aaatattctc aaaacttttt
      721 aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac
      781 cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa
      841 acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa
      901 actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa
      961 acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa ttattaaaaa
     1021 agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag gattctacaa
     1081 gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca
     1141 gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt
     1201 aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta
     1261 ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca
     1321 tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt
     1381 gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt
     1441 ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta gatatactac
     1501 tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg tatcgataag
     1561 gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct aagtgtggca
     1621 acaatattgt att
//
LOCUS       DAJSMI010000273         1606 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJSMI010000273 DAJSMI010000000
VERSION     DAJSMI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.278"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(1..81)
                     /locus_tag="ON430_002782"
                     /pseudo
     CDS             complement(1..81)
                     /locus_tag="ON430_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            430..1515
                     /gene="xerS"
                     /locus_tag="ON430_002783"
     CDS             430..1515
                     /gene="xerS"
                     /locus_tag="ON430_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCR3220652.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          611 a          275 c          218 g          502 t
ORIGIN      
        1 taaaaaaatt gtatctccag gatgcaaccc attttcttta agaaattcct tttaaattgt
       61 taatactact gagtgcccca cttctctgaa ttttctttaa cttctccctc ccctttttta
      121 tttgtatata cattcatata cgcttgttaa tatcaaatca accgtagtct ctcttatgtc
      181 taattatata gaaagattgt tctttctata taatttagac aatttaaaaa attaagattg
      241 cctccttctg tttcctttcc tattcatgta taccatttat tttaaattat taaatctata
      301 cctatttatc tgcttttttg aaataaatta ataaatttca aagtatacca attgacaaaa
      361 cactctataa ttggtatagt ccaattatag agatactcct ctcccctact tatcgaaagg
      421 atgaataaca tgaaaaatac agaaatatat caaaaaatag atacaatcat acttcatatg
      481 cccgattata taaaagattt tgttcaagat cgtgaggata aagatcaatc acctcgaact
      541 actttagaat atctaaaaaa ttataagctc ttttatgaat ggcttctgag tgagtctatc
      601 gttcccgata cttcaatcac ctcgataaga cagttaactg cctatgatct aaacttgtac
      661 aaatctcatt tgaaacgtcg cacaaaggaa aatgtaaaaa aagagacaac aaaaactaag
      721 ctcaaagata ataataactt aggtctatct acttctacta tcaatagaaa tatcacagca
      781 ttaaaggttc tatttaaata tctttcgaaa agttccaata atcctttagg aaaaccatac
      841 ttagaaaata atcctatgga tcaagtagca actatcacag ataaaactac gttagctgcc
      901 cgtgcaaacg caatcgagaa aaaattattt ctagacgaag atacacaaaa ttatctagat
      961 tatattgcca atgaatataa aaaaactttg agcaaacgag ctttgatcta ttatcaacga
     1021 gatgtagaaa gagacctggc gatcaatgca ttgattttag gatctggatt aagactctcc
     1081 gaggttgtaa atattaattt agaggaccta tctttagaaa agaataatgt ggtagtaact
     1141 agaaaaggaa ataagagaga cgcagtaaat atagcagcat ttgcaatgga atatttagct
     1201 aattatctag ctattcggaa agaacgatac aaagtaacag aaaacgaaaa agctttattt
     1261 ctagcaattt atcaaggaga agccaagcgt atatccggta ttgcaatcga acggatggta
     1321 gcaaaatatt caaaaggatt cagagtccaa gtcagtccac ataaattacg tcatactttg
     1381 gctactagac tctatcaaca gacaaattca ttggtattaa ctgcccaaca attgggacat
     1441 acaagtacaa atacaacaac tttatatact catatagaca atgcagctac aattgatgct
     1501 ttgaacgctt tgtaaatgta gtaaaaaata gtataataat gaagcctgtc tctaaaatta
     1561 gataaaagcc caactttaaa ggcaagcttc attatcattt ttatat
//
LOCUS       DAJSMI010000274         1594 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJSMI010000274 DAJSMI010000000
VERSION     DAJSMI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.279"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(180..917)
                     /locus_tag="ON430_002784"
     CDS             complement(180..917)
                     /locus_tag="ON430_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3220653.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            complement(968..>1594)
                     /locus_tag="ON430_002785"
     CDS             complement(968..>1594)
                     /locus_tag="ON430_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3220654.1"
                     /translation="KNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIVSDIFNNV
                     KSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSFNPLKGQI
                     PHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILSSTSVSSR
                     AQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
BASE COUNT          481 a          314 c          257 g          542 t
ORIGIN      
        1 gttccgttta ttgaataagt accctgaaat atctcggctc cgtttagttt attgctaaca
       61 gtagctgtta accagtctga caaagcgcct aaaccttgcg tatcatataa atgttcagct
      121 aaattattcg cgtcattttt atcgtaaata gttattaaat tatcgatcat ctatttcacc
      181 taccttaacc cgttacgata aatttgtatt ttgctcaaac cagtgcaatt aaaatgattg
      241 atatccactt gcaatgtcgg atattgcatg gtcttcattt tgttggaccg atctaaaata
      301 tctccgtccg attgctcttc gtagcaaagc atcaaatcac tatcaatgac tacatcagtt
      361 cctgctacta agccttcgaa actaaacaca taatcattta agatgaactg gcatgaagta
      421 gctgaaggag tgatgataat ctttggaaaa ctttcttcta aactattatt cagcaagtta
      481 aatgactgtg gtttatctac ggttataggt acatcttctt gaactcttgc gaatggtttc
      541 gcagtaatat ttacatcgaa ctctccccat tcaacaatat cgttttctgc atccccaata
      601 tcgatagtct ggataacgta atagacgttg ggatcgtcag agaattctaa tttctttgca
      661 tagtttaacc aatgacgcat gatataaaac gattgcttga acgcttgatg gtcttccaca
      721 tcctctaaat agttatagtg caatgtaaac gacatatctt caaacgagta atcttgtact
      781 aagccaccta acctacctaa aacagaagtt tcaactctct gtctttttgg agaaggtatg
      841 gttggtcttt cagctaaagc caatttatgc aaataatcag gaaatccatc gattatagaa
      901 tgtatacaat cagtcatttt ttcacatcct ttttaatact aaaaaaacag gagaaatact
      961 ctcctgttta acgccatgcc gaagcattat cattttgaat ttttgtaatg ctatcaatga
     1021 tttgttgagt tgtttgcttc atagtaacct catctgcgtt accatcaatt gtgaaattga
     1081 attcgtagtt attcacaggt tgaatcgttt gtgccctaga tgaaactgat gtgctactca
     1141 agatacgatc accaatttct tgcaaaacag atcttttcaa aggtaaaact gcttcaggtc
     1201 ctgcttcacc gacaccgttc attccaccta gtaaagttgg tttagtaaag atacctcctt
     1261 tagcatgcca ttttacacgc aaatggggga tttgaccttt tagcgggtta aagctgcctt
     1321 ccatgataaa ttccggtaac ggaatatgtg gtatagaaat attcaaatta tcaaaaatgc
     1381 cactgatttt gtctctaatc caatcaattg gagcgctaac agtctttttg atgccttccc
     1441 atatgttagc aattgtactt ttaacattat tgaatatgtc ggaaacaata cctgttagat
     1501 tggaccaacc gcttgaaatt gcattttttc catcgtttac tttagagcta atagtgcttg
     1561 taattccatt ccaaagattc aaagcagtat tttt
//
LOCUS       DAJSMI010000275         1591 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJSMI010000275 DAJSMI010000000
VERSION     DAJSMI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.280"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(19..1398)
                     /locus_tag="ON430_002786"
     CDS             complement(19..1398)
                     /locus_tag="ON430_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220655.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHP
                     DMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKL
                     VQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQ
                     PRREVQHSH"
BASE COUNT          612 a          223 c          259 g          497 t
ORIGIN      
        1 tctttcttaa ataaaaatct agtgagaatg ttgtacttct cttctcggtt gtttttgttt
       61 agaaaaagta aatcgataaa gtaaaaataa aatcaaagta acgatagata aatatcttga
      121 agcaattgct agttttgtgt actgataagt aataacgact ttatttatac ctggaggaat
      181 agttaattca gttgtgccaa acttagataa ttttgtttgt actagtttgc cattaataga
      241 agccacttgc cctttataac gataaatagg tgtcgtcaag acagtatttt tattattcga
      301 attatctaat tcaattgtaa gtttgttact cataaaatga gttgttggat ttatttcctg
      361 gttacctagt aaatattgat cattattaat catatcagga tgatagattg actctttgtt
      421 tgcataatca gtatgattgt aattttcagc tatagcaaca gcgtcttcag gtgctataag
      481 tgttaatgaa tctttgttag catggataga attgcttact cctgagagat gcaatattag
      541 tgcaagaaac gtaaagacca ttattttcca tgaattattt ttagtagaaa aaatataata
      601 gatcactata ctcatcgtat aggctataaa taaagaagaa aaaccattta accgccagac
      661 aaattgtaga ctcttaactg gtgtatggtt aaaaagttgc caaggaaata aattggttga
      721 acaaaaaaga acaaatacac caagaaagaa aataaataaa tcatcagcag ttaatttttt
      781 tataaaaata aaagcaccaa tcaatccgag aaataagaat aatccaatag tatagttgtt
      841 atagttatta tttaaaatgt gtgtcagcat atcactagga gccataccac ttaattcttt
      901 tcccagtggg actttcaatt cttgtgctct agattgttga ataaaaggaa ttaagaatcc
      961 cgcacttaaa aaaaaggtga ggacggtagc ttttaaaaga cttaagagtc tagcgatttt
     1021 ttggtcccaa aaataaaaag aaagaaacaa cgttcctcca ataaatacag aaaccatagc
     1081 gacagaaagt aaatgagtat acactactag cgtcatgcca atagacaacc agtaccattt
     1141 ttggtaatct ctaatataaa tttcgtaaca ccccattagt atcaaaggca aaaatgttaa
     1201 agcgacagct tctcccaatg aagcacgatg gaatatgtca atggcacgat aggcggaaaa
     1261 tgtataaata atcgaaaata acagcgaaat ataacgattg ttttttattt gtttcataga
     1321 aaaataagaa acgaccattg ttaaaaaagt gataaaaaaa tagtagatat tataacttaa
     1381 aacaagattt cccatcattt tataaaataa aaaagctgga taaagagtaa gccatggata
     1441 aaaaatattc atcattgttc catgatgatc gaagttattg aagtttactg gagaactcca
     1501 tacattatct agtcctatga ttcgagataa atgaaagtaa gtatcctgat tgacactatg
     1561 atagattccc atagacatga atagagggat g
//
LOCUS       DAJSMI010000276         1563 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJSMI010000276 DAJSMI010000000
VERSION     DAJSMI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.281"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            46..468
                     /locus_tag="ON430_002787"
     CDS             46..468
                     /locus_tag="ON430_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220656.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            478..681
                     /locus_tag="ON430_002788"
     CDS             478..681
                     /locus_tag="ON430_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220657.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            892..1476
                     /locus_tag="ON430_002789"
     CDS             892..1476
                     /locus_tag="ON430_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3220658.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          554 a          223 c          291 g          495 t
ORIGIN      
        1 tattaagaaa acgttttctt atttttgatt tcgaggtgat agacaatgaa aaataagttt
       61 attttatctt taggtatttt aactattgga attagtgtaa ctggaactac aattcctaca
      121 tttgctgata gtacaaatga accaactact gtagtagata ataaccaaga attaactcct
      181 ttagatagga acggaatagc cattggtaac ccaactaacg acttaactgt tactgaaaat
      241 tatttaatag tttctccatt acttgctcga gcagcttcta aaacgatacg agtgacagga
      301 acgatgattt ttgataaagt tggttggcct tctagacctc ctcaatcacg gaattggaaa
      361 gaaaaaagaa atggttattg gtactatgga aaattgtatt tacaaagtta taaatcagct
      421 ggaaacaatt ggattgcgac ttattcagga accttaacta gaaagtaggg attttttatg
      481 ccagaatttg tgaacacacc aaatttttat caagatagcg atattctaag taaaattaat
      541 ttaccgaaag aagaatcagc tttacaagtt cagcgaactt tacaggagac acaaaaaatt
      601 caaaaatcaa tttctaatct agaagattct gaagaaaaaa gaaaaaaaag agaccaaaaa
      661 acatatcaaa aagaaaaata atgattttaa aaaaagagtg tctagtcgac attctttttt
      721 tctaattgaa agtgttcata aaatcaccca tttctggaca ctttcttttt ttgctttatt
      781 tttcaaaaaa acaattcatt tcatgttcat aaacccataa caatatctat gttcagttaa
      841 actatatgga taaagttatg ttacaataaa attaatgaag gagtgatttt tttgaaaata
      901 ggatacgccc gggtatctac tggtttacaa aatttggatt tacaagaaga cagcctaaat
      961 aaatttggtt gtgaaaaaat ttttactgat catatgagtg gggcaaaaag taatcgacca
     1021 ggattagaaa tggccattga ttttgttcgc tctggcgata cacttgtcgt ttggcgctta
     1081 gatcgtttag gtagaaatat ggaagatttg atttcaatcg tcaatcgact gaatgagcgt
     1141 ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg acaaatcaag ttctacagga
     1201 cagctcatgt ttcatttgtt tgcggcattt gcagaatttg agcgtaattt gatcctggaa
     1261 cgatccgcag caggacgagc agctgctcgt gcaagaggac ggtttggtgg tcgtcctgaa
     1321 aaattgtctg aacaagattt ggaattactg aagactttag tggatagcgg tacacctatt
     1381 aagactattg cggaacggtg gaacgtctca agaaccacca tttatcgtta tttggcaaaa
     1441 atggaggaga aaaatggaaa gacaaattct cattagtttt tgtcatttaa tgctgaaaat
     1501 ttttgccatt tatctagaaa agtgacaata gtatgagaat tttttgtaag tgtctactga
     1561 taa
//
LOCUS       DAJSMI010000277         1560 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJSMI010000277 DAJSMI010000000
VERSION     DAJSMI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.282"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(113..598)
                     /locus_tag="ON430_002790"
     CDS             complement(113..598)
                     /locus_tag="ON430_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HCR3220659.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(600..>1560)
                     /locus_tag="ON430_002791"
     CDS             complement(600..>1560)
                     /locus_tag="ON430_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HCR3220660.1"
                     /translation="TRDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERI
                     LSDIRWVLRENQRPHYCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSI
                     NVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSE
                     SGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENII
                     AESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQE
                     NFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLINSIQED"
BASE COUNT          503 a          311 c          160 g          586 t
ORIGIN      
        1 tcctaattca gtttgatgaa ttaactgata aaaaaatatc tactatttaa agatacaggg
       61 ataagtaagt aaaaaaataa catgtcatta gatagtagac agttccaact ctttatttac
      121 cttttttgta tgctgcactg tcaatccaag ttcctaaatt gttgtatcca tcatcactta
      181 tataatcata ttggccatca ataatttcgt caaaccacaa atattctcca tctggttttt
      241 gcccaattat tttaattact gattgactag tagttgtcat ctggttaaaa tcttttattt
      301 tacctatatt aacggccaaa atagcatttc ctcgcttata actctcctgg atttcatatt
      361 taacatattc tcgatcaaga gtttctttac ctatcagcac cacagtcact gatgttccaa
      421 ttaattgatt atctatccag ttttttatag caatatctcc attacgttta actttctcaa
      481 attcagcctt atctacaaaa ccagcttcct ttgttcctcg caaagtccag ctgtttctaa
      541 ctaccattgc tcttgttaca tctgcataat ggaaactaaa aaaaaccctt ctcaccatat
      601 taatcctcct gaatactatt tattaaactg acaatttgtt caactttttt atctacttct
      661 ccttcagtat ctaatttttc tagcgcttga aatttacttt ttaaggaatc atcaaaataa
      721 tcactgaaat tctcttgaat cttatcaaac aaaaccttac ttgcaccttt ggtaaatcca
      781 atcggaacta ctatattttc attttgaaca gataattcaa attcttcaat cattccatct
      841 gctgtaacta tttcacctga ttcaagtttg tttccaaaaa caaaaatact aatcccagac
      901 tctgcaataa tgttttctcg atatcttctc cactcatgag gcatatcaag gggaagtggt
      961 cttaaagaaa gtacgtcttg tatgttattt tttcttaaat ttcttctctg ctttagaact
     1021 cctgaaacaa tactattacc aattccaagt ccgtaacctg atactattct ataaccggat
     1081 tcagaaagcc tatatgctaa tttctcgaca aattttaatc ctttcgattc agatgtaaat
     1141 ggagtataat caacagcact tcccgaaata aaaatagttt ttctcaaata cttttttcta
     1201 atacttttca aaatatcagt aatctcagaa taatcatcta cttcaacaac gtttatcgaa
     1261 aatctggaca aatcattgat ttgtagtctt ctttttgtca gttcataatt atatctctct
     1321 tgatcaaaaa aatcctcaga atcgacaaaa ttttccttta atatttttct cattatacaa
     1381 taatgaggtc tttggttttc tctcaaaacc catcttatat ctgataaaat tctttctaaa
     1441 ttagggtctg taaaactaaa tcctaaaaag agaaaagttt ttgttaacaa atctcctttt
     1501 aaagtttctt taaataacgc gtacgaatca tcatcatatt tttcataatc atctcttgtt
//
LOCUS       DAJSMI010000278         1538 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJSMI010000278 DAJSMI010000000
VERSION     DAJSMI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.283"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(100..1425)
                     /locus_tag="ON430_002792"
     CDS             complement(100..1425)
                     /locus_tag="ON430_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3220661.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRQKLAQMIQEHIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCFFLFDKYYEGQEVRHVGITYSKLIYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTAIVHASSMLEGARSITRSTLVGGHAGGNGGLKND"
BASE COUNT          428 a          334 c          248 g          528 t
ORIGIN      
        1 aaattattat ttgcacgttg ctcattgtcc gcatcgatct cttccgtgtg atcggacaaa
       61 tagaatcctg cccatttcaa ccgtcctcga tcgttataat caatcatttt taagcccccc
      121 atttcctcct gcatggccac caaccaacgt gcttctagta atactacgag cgccttctaa
      181 catagaagaa gcatgtacaa tcgctgtaaa accatatttt tgtctgatct tatcaataat
      241 cttatccagg ttttcttcat cgatttgttt ttgcggatca ctaaataaat ccagctgaag
      301 cgaatctgta taaattagct tgctataggt tattccaacg tgccttactt cttgtccttc
      361 ataatactta tcaaacaaga aaaaacaata attctgcaat tcctttgtat tatctgtgat
      421 agggattttc atctggtgtg aaaatcctgg tctcgtttct aagatcgacg taccaatgtt
      481 taaatgaacg catcctgttt tacaattatg ctgtctgata cgaatggcta cttgttccgc
      541 catttctttt accaccagct cgatttcatt tcttctagta taatctcgtg gtaacacttg
      601 tgaattacca taagattttt cctttgtcgg ttctggtggg atcgctatat ctgttcgatc
      661 gattccgtta gcatggaaat acagctgtaa tccaatcaca cctaatctat tcttaatcgt
      721 ataaggattc cagttagcaa ggtctctaat gctcaaaatc cccatttggt tgagcctttt
      781 tttcattcgc gaaccaatgc cccaaaagtc cgtcatttca ggaatattcc atactttttc
      841 aggaacgttt tcgtacgtcc attccgcaac aaatccttca ttatgctttg cttcattgtc
      901 taaggctagt ttagccaaaa ggggattatc gccgacacct acagtagcga tcaatcctaa
      961 ttcttcttta atatgttcct ggatcatttg agcaagtttc tgcctgcgct gacttcttgt
     1021 tccttcagtc gtaaaaagat tcagtgatct ggtcactttt aagatcgatt catcgatcga
     1081 atagatcaat aaatcttcgt ctgctacata ccttctgaaa atattgttta cctgcatgtt
     1141 tcgcttaata tagagattca tgcgtggtgg aacgacatgt aacgttttag gaaaaggctg
     1201 tggtagatcc cgtggtctgc tgacattaga aataccgtac ttcttctttg cagctggtga
     1261 agaagctaaa atcaaccctg atccagtgtt gtcagcacga ctcataacaa caagttctgt
     1321 tgttaatgga tctaagtttc tttctataca ttcgacactt gcgtaaaagc ttttcatatc
     1381 gattaaaaag taatcattta ctggctcttt tgagtaatcc atcataaata acactccatt
     1441 ctttcatatt atacaaacat atgttcgata ttgtaaagaa gaaatagcta aatacataaa
     1501 aaaagaagca taggtatgct atcaccctgc ttcttctt
//
LOCUS       DAJSMI010000279         1538 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJSMI010000279 DAJSMI010000000
VERSION     DAJSMI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.284"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            131..505
                     /locus_tag="ON430_002793"
     CDS             131..505
                     /locus_tag="ON430_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3220662.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ON430_002794"
     CDS             complement(619..1248)
                     /locus_tag="ON430_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3220663.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ON430_002795"
     tRNA            complement(1382..1454)
                     /locus_tag="ON430_002795"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          522 a          231 c          267 g          518 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaata
//
LOCUS       DAJSMI010000280         1532 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJSMI010000280 DAJSMI010000000
VERSION     DAJSMI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.285"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(52..1518)
                     /locus_tag="ON430_002796"
     CDS             complement(52..1518)
                     /locus_tag="ON430_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCR3220664.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          501 a          325 c          289 g          417 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aa
//
LOCUS       DAJSMI010000281         1515 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJSMI010000281 DAJSMI010000000
VERSION     DAJSMI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.286"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            179..>1515
                     /locus_tag="ON430_002797"
     CDS             179..>1515
                     /locus_tag="ON430_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3220665.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTN"
BASE COUNT          550 a          210 c          273 g          482 t
ORIGIN      
        1 tagaatattt cctcataaat ttttccattt tttgcaatat tctcaaaaaa aagataaaaa
       61 atgagaataa ctgatgaaaa aacaagcata atccatcata aaatcagtat aggtgaaaag
      121 atgaactttc atctgcatat caagtaattg gtatgaggca ttttttgagg tgtaggatat
      181 gaagttggat taccttatag aagaggaaga tagagaaaaa ctgctgctag ctagatattt
      241 agaatttgag aaaaagaatt atgtagtgct gcctaagtta tatgaaacat taggtttgtc
      301 taaatttaaa acaagaaatt atatcataga attaaataaa gaatttactg aattcaaaac
      361 aaagccagag atccaaattt cagaagaagc agagatccat gtcgaacgtc tgaatttgac
      421 ggatatcaaa aatttaagaa gaatctactt tcaacgatca aaaattgctc gactgttaac
      481 ggagatcgtg gattcaaaag taacgatcga aaaatttgca gagaatcatt ttttaagcat
      541 atcaagagcc tatgtaaaaa gaagagaatt agtctctttt cttgaacagc atcaaataaa
      601 gttaaagaaa aacatattag ttggggaaga aataaacata cggaacgtat tatttggggt
      661 atatttcggg gtctttaatg gtttttctat gccatttaga gaaagcgtca tgaatgaagc
      721 aaagagtatc gtgcaatatt ttgaatatct gttcaattta aaactgtctg caacaaaacg
      781 aatcaaattc aatttgctaa ccactattag tttgttgagg gcctatggga atcaaccaat
      841 cggaaaacgt ttcttttcag atgaatactt taagagctca gaaggaatga agatcatcaa
      901 ggatttatca catatttctt ttcttcatat ccctaaagag cagatcattg atgagatttc
      961 ttatatcatg ttgtttttga tcacggaaga agaatttgaa ttagatggcg catttggact
     1021 tgcggaagct cattttcatg atatccacat gattagtcaa aaaatgtcag tggaattgat
     1081 tgatcaatta ccattttcga ttgaaatctc tgaagaagaa agactatata tacgaaaaga
     1141 acttcgtatg ggtatgatgc atgtcaatcg aaaaaattgg atattcgact ttgagatgac
     1201 tagctttacg tctaaaaagc agctacagtt tttcttcgag acgtatccaa tattcagtac
     1261 agcaatccaa aaggtcatac aagagaataa acgctatttc ttgggaaaag atgaacgttt
     1321 catgattcgc ttattttatg attatctctt tttattggtc ggtatttgtc cggtatcata
     1381 ttttgaagca ccgattcatg tctgtattga tttttcgcaa ggatctgctt ataccgatta
     1441 cattatttct caagtgaaag gatttaaaaa ttacaatatt gtgattgaga gtcgaatcac
     1501 ttctcgtacg aatat
//
LOCUS       DAJSMI010000282         1505 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJSMI010000282 DAJSMI010000000
VERSION     DAJSMI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.287"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            49..1437
                     /locus_tag="ON430_002798"
     CDS             49..1437
                     /locus_tag="ON430_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HCR3220666.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
BASE COUNT          599 a          182 c          251 g          473 t
ORIGIN      
        1 tcaaaaaaaa accaaccgaa agatataaaa gatattgtag atacacgaat gtataaagaa
       61 cgtgatgact taaactatac gcttattatc aataaagatg gaaactattc atggcgtcct
      121 ctcgttctac tacatcctat tctatatgta gatttggtta attgtctcac taaagaagac
      181 cattgggagg agttaaaaaa gaggtttagt gaatttaaaa aagataaaaa tattaactgt
      241 tattctatac ctgttgaagc ttccaaaggt tcaaaaagga cagatcttgg tgaaacaata
      301 ctaaactggt gggaatattt tgaacaaaaa agcttagctc agaatattaa ataccaatat
      361 tgcatgttta ctgacatcgc tgattgttat ccatcaattt atactcactc tataacatgg
      421 gccatgtatg gtaaagaaaa agttaaaaat gatgaaaata agtgttcggg ctcacttggc
      481 gataaaatag ataaaaaaat atctggaatg caacacaatc aaacaaatgg tatccctcag
      541 ggttccgttc ttatggattt tgtagcagag atcgttctag ggtatgttga tttgagatta
      601 tcgaaagaat taaaaaaatt agaaaattta aaagattaca aagtactcag atatagagat
      661 gattatagaa tattttcaaa taatcgtaat gatttggaac aaattatgaa agtgttatca
      721 gaagtcttat atgatttaaa ttttaagtta aattctagta aaacaaaatt atcagatgat
      781 attatcatag attctatcaa aaaagataag ctctattggg aagaaattaa ggcaagtata
      841 agaatgaatg ccacaaactt agatggtgat agagaaattc gttatagtat aaatttacaa
      901 aaacatttat tggaaataaa aaagttagca gataagcatc ctaattcagg ccaattgaaa
      961 aaagcacttt ctgagtttaa taaagaacgc attattaaaa tcacttcaaa gccgaacgat
     1021 cttttaccac ttattggtat cctggtagat atcatgataa ataacccggg ttctttgccg
     1081 cagtgtgtct taattatagg gcagttatta aatttcgaaa atgagagtaa ggctagagaa
     1141 atagttgaaa tgattataaa caaatatgct ttaaagccaa atactgaatt tatgatgata
     1201 tggtttcaaa gattgaccac catttttgga gaaatgtttg atagtgtttt atcatccgaa
     1261 tttgtatcta aaatcaaaga tcctgtctct aatcagatgt tttctgtaga ctggctaaat
     1321 gaagatgcta agaaatcttt ttatgaaggg accattaaag acgaagagaa atatattaaa
     1381 atgagtaaga aaatatatat tactgaattt gatgatttta atgattatcc tttttaagtg
     1441 aaaatttaga atgaatataa aaaacattac aatctaattt ttttaaaaaa atgttatatc
     1501 ggaag
//
LOCUS       DAJSMI010000283         1487 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJSMI010000283 DAJSMI010000000
VERSION     DAJSMI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.288"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..1412
                     /locus_tag="ON430_002799"
     CDS             <1..1412
                     /locus_tag="ON430_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3220667.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
BASE COUNT          586 a          233 c          326 g          342 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatggga
//
LOCUS       DAJSMI010000284         1471 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJSMI010000284 DAJSMI010000000
VERSION     DAJSMI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.289"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..1245
                     /locus_tag="ON430_002800"
     CDS             <1..1245
                     /locus_tag="ON430_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3220668.1"
                     /translation="KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSI
                     DSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEA
                     YKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEI
                     KGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDS
                     VIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLF
                     LWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNIL
                     NKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLV
                     SLIISSFNILRRNPKELLIDNN"
BASE COUNT          575 a          178 c          264 g          454 t
ORIGIN      
        1 aaagaaacat ttgctaatat aacaaatagt ttttctatgg agataaatag acaagtaaat
       61 ccaggaacac ctagaggtgg gggaaatgta aaaggtgaag atattaaaaa gatatctcaa
      121 acagatagta tagactccta tgtaaaaaga ataaacagtg ttgcagattt agttgattat
      181 gatattattg aaactcaaga gactcttgcg aatcaatcac ctgaaagagc aaagaatttt
      241 aaaagaacag ttatgttaac tggggttaac gactcaacaa aagaaacaaa atttgtatca
      301 gaagcatata aattagtaga aggaaagcat ttagaaaatg aagataaaaa taaaatctta
      361 atgcataaag atttagctga aaaaaataac cttaaagttg gagataagat aaaaataaaa
      421 tctaatttat ttgatgctga taatgagaaa ggtgcagatg aaacagtaga agtagaaatt
      481 aaaggtctat tcgatgggca taatagcggt ggagtaagtg cagcacaaga actatacgaa
      541 aatacattaa tcactgatat tcatacagct gctaaagttt atggtaatac cgaagataca
      601 gctgtatacc aagatgcaac attctttgta aaaggtgata agaatcttga tagtgtaata
      661 aaagatcttg ggaaattaga tataaattgg agagaatata atttgattaa aagttcatca
      721 aattaccctg cattacaaca atctatatca ggtatttact caatttcaaa taaattattt
      781 tttggctcat taatatttgc aggagttgta gtttcattat tattattctt atggatgaat
      841 gctagaaaga aagaaatagc agtattacta tcattaggta tatcaaaatt agaaattttt
      901 ggtcaattcc taattgagat ggtatttata tcaatccccg cattcgttgg ttcctatttc
      961 ttagctcaat atacagctga caagcttggg aataatatat tgaataaggt tactggtgat
     1021 atagctaaac aaatagctaa acaatctgca tctagccaat taggtggtgg ggcagaagct
     1081 gaaggattta acaagacatt atcaagttta gatatcaatg tattacctaa attcatgatt
     1141 tatgtagtta tatttatgag tttagtactt cttgtatcat tgattatttc ttcattcaat
     1201 atattaagaa ggaatccaaa agaattatta attgacaata attaaaattt tcggtgcttt
     1261 ttagcaccaa aaatgagagt gtaatataaa ccgtgtaagt aaccatcaat cctaggcggg
     1321 tggttgcttc acggttttta tttttctaaa ataagatcaa gaggtgatta cacgatggct
     1381 agaaagaaaa gaaatcctga tgctgagaag ttagctgaat ccattttgaa tacttatcaa
     1441 cccgaatctg tagaggatat gcaagatgtt t
//
LOCUS       DAJSMI010000285         1456 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJSMI010000285 DAJSMI010000000
VERSION     DAJSMI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.290"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..626)
                     /locus_tag="ON430_002801"
     CDS             complement(<1..626)
                     /locus_tag="ON430_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3220669.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPR"
     gene            914..>1456
                     /locus_tag="ON430_002802"
     CDS             914..>1456
                     /locus_tag="ON430_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3220670.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEE"
BASE COUNT          505 a          212 c          278 g          461 t
ORIGIN      
        1 tttctaggaa agtgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattaaaa aaaagaaaaa gtattggcaa cagaaggcgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata
     1201 tgtagaaaag ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa
     1261 gaattatgga catttagaga agtttgaata taaggatcta tcggttaatt ttaatagcta
     1321 tacagctaaa ataaatggtg aagaaataga tgtaaatgca aaagaactag aagtattaaa
     1381 atgtttattg gataataatg gacaagtgtt aacaagaatg caaattattg attatgtatg
     1441 gaaagatagc gaagaa
//
LOCUS       DAJSMI010000286         1455 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJSMI010000286 DAJSMI010000000
VERSION     DAJSMI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.291"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..237
                     /locus_tag="ON430_002803"
     CDS             <1..237
                     /locus_tag="ON430_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HCR3220671.1"
                     /translation="QKLSLGEVMDLVSNDGVYRYKVTRKFIVPEYFKLIDGVPEENSF
                     LSLPKKGEKPLLTLFTCVYTSQGKERYVVQGELQ"
     gene            290..>1455
                     /locus_tag="ON430_002804"
     CDS             290..>1455
                     /locus_tag="ON430_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR3220672.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWG"
BASE COUNT          525 a          232 c          325 g          373 t
ORIGIN      
        1 caaaagttgt cattagggga ggtgatggac ttagttagta acgatggcgt ctatcgttac
       61 aaggtaacgc gtaaatttat cgtacctgag tactttaaat taatcgatgg cgttcctgaa
      121 gagaactctt tcttgtcttt gccaaagaaa ggagagaaac ccttattaac gttgtttact
      181 tgtgtttata cgtcacaagg aaaagaaaga tacgtggtac agggagaact acaataacaa
      241 ggttttaatc aacgtgagtc gttgaaatat gaaatgagga gagtaaaaaa tggcaataaa
      301 tcggtatgta aaaaaagctg gaatgcttat tggaatgtta ggaatcattg caggctgctt
      361 tcagggatat cgagcatatg cagaagatgt cacgcaaaaa acgccaccag agaaggtcaa
      421 tatcacggtt cataaattaa tgtacgatca aggaacgcaa ttgaatgttg atattgacgg
      481 gatcaaaaac gatggttata cgcacgatca atatcctaca ggtgtaacga aatacaacaa
      541 ggcagattat ggtgatgttg agtttacgtt aggaaatatt acggatcaag tattaccgac
      601 tgaagattct gatctaacca acgcgaaggt ggacgaaatc gttaaagatg tagaagacaa
      661 gggatctaac agtgagtacg tcaagaatgc aaccaacaaa attacttcag ctgtagatga
      721 aaatggtgag atcacttttg ctgatcaacc agcctatgtc aattccaaag ggaatgtata
      781 tgtggtttat gaaagtaaat ccgctgctgg attagtcaca caaaaagcaa aaccaatggt
      841 tgtgattgcg ccaatgactg ataatacagg ttcaggtttt ttaaaagaca ttcacttata
      901 tcctaaaaat attgtttcta agttgagttt tgaattaacg aaatttggtg acgatggcac
      961 agctcaaagc aaacaaacac ctttaaaagg ggcaaaattt gagttataca agggcgaacc
     1021 tggaaaagga acaaaattag gagatcttgt atcggatgat caaggaaagc tgactgctac
     1081 tgatttgacg ttaggaaaat actattttgt ggaagttcct tcagaagttg tagtgggatc
     1141 agataaagaa ccaacagccg atcaatattt attaggcgca gacgctcgca acgatgcgca
     1201 caataaatta acctttgaaa ttacgaatga tggcgtgaca agtgacttaa aagcttcata
     1261 cgtaaactat aaagcaccag tgatcgataa aacagtaaca aatggaacag gacaagaaca
     1321 ttctttccaa attggtgacg cagtaaatta tcaaggaacg attcatatcc caacagacat
     1381 tgcaggcgga gcagacggta tcacagtaaa cggagtgaaa agcgaaactt ctccttacag
     1441 tgtatttaaa tgggg
//
LOCUS       DAJSMI010000287         1429 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJSMI010000287 DAJSMI010000000
VERSION     DAJSMI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.292"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(45..452)
                     /locus_tag="ON430_002805"
     CDS             complement(45..452)
                     /locus_tag="ON430_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220673.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(471..1289)
                     /locus_tag="ON430_002806"
     CDS             complement(471..1289)
                     /locus_tag="ON430_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3220674.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          427 a          233 c          200 g          569 t
ORIGIN      
        1 atctagtata acttatactt taattgaata ccgcatgaaa aaaattattt tggtaatttt
       61 tcaaactctt cttgtatttc ttctcttatg tgttcttctt ttgctactcc tagtctatgc
      121 tggatatatg agtctgctaa tgtattaaca attttttcga aagaaattct ccctttttca
      181 ctttgtaact ccgtcatata gtcttgtaaa attgagagtt tgctatttaa tgccggagag
      241 atctttttag gaactgatac cttgtttctt ttatcaggaa ttgaaactgg tcggcctctt
      301 tttttcaatg taatatttct tgtgttactc aatgcactat tgctaaagtt atattcaccg
      361 cttattttca catcttggcg tttgttattg ggtaataatg gctcaaattc accattctcc
      421 atcattttag gaatatcaaa atctttaggc attacttatg caactcctta ttatttattt
      481 aaaaactctt gtctagcatc tagttctgcc aaaatattta taaatacttg gtgtgcttta
      541 taatcccatt gatcaaaatt tccatttttg tattttctta aatgtatacc aattttagaa
      601 taccttctca atcttttttg ataatgaatg agatttttgg ataccacggt tccgtatttt
      661 tcaattgctt cttctaggat ttcttcatca aaatcatcat ttggttctac catacaaggt
      721 acaatatcta taatttgcaa atcaagatcg tatctatcga ccatgaattt ttgataattt
      781 acatatttgt tgactccctc taaggattct tcagctgttt gaaatgaaat tatcgaatag
      841 tctgaagctg ccatagcatt atcggaaaat tcgctaatgg ttggtggtac atcaataaat
      901 atttcttgat aattgttctt tattggaaga agtaattttt tcaagacagt aacttggtct
      961 aattcatttt catgtttatt agcaaaagcg gagaatgatc gaaaactagt gtcacatgcc
     1021 agaaggtcaa gattttcatt taccttaata atactacttg ctaattctcc atttgcgaca
     1081 ccatttgtta tagtggtttt gatatcggtt ataccgaaag ttcttgctat gacttgtgtt
     1141 aagttcgctt gagggtctaa atcaataata agcgtttttc tatttcttat cattgcattg
     1201 tcataagcaa gcatgctgac tatcttagtt ttacctactc ctcctttaaa attgccaact
     1261 gtaaatgtag tcccttgaaa agtatccatg taaatcctcc caatctttaa ttgataatta
     1321 aagtataact tatatatcgg aaatggtcaa cattaaaagt ataacttaaa taataatatc
     1381 taaaaacgtt tggatatagc ggtttttaat aataaaagta taagttata
//
LOCUS       DAJSMI010000288         1384 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJSMI010000288 DAJSMI010000000
VERSION     DAJSMI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.293"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            66..800
                     /locus_tag="ON430_002807"
     CDS             66..800
                     /locus_tag="ON430_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HCR3220675.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
     gene            1254..>1384
                     /locus_tag="ON430_002808"
     CDS             1254..>1384
                     /locus_tag="ON430_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3220676.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVC"
BASE COUNT          518 a          209 c          216 g          441 t
ORIGIN      
        1 aaataagtgg ttataatgga ttgttaatat aaacattcat tataacctca taggagtggg
       61 ttataatgaa caaaaaaaat ataaaagata gtcaaaactt tattacttca aagcatcata
      121 taaatgaaat tttgagaaat gtacatttaa atacaaatga taatattatt gagattggtt
      181 cagggaaagg tcatttcacg tttgaattag ctaaaaggtg taattatgta accgccattg
      241 aaatagatcc taaattatgt aggataacta aaaacaaact tattgaatat gagaacttcc
      301 aggttatcaa taaagatatt ttacaattta agtttcctaa aaataagtca tataagatat
      361 atggaaatat accctacaat ataagtacag atataattcg aaaaattgtt tttgaaagca
      421 cagctacaga aagttattta atagtggaat atggatttgc taaaaggttg ctaaatacaa
      481 atcgttcact agcactattt ttaatgacag aagttgatat atccatatta agtaaaatcc
      541 ctagagaata ctttcatcca aaacctagag ttaatagctc gttaattgta ttaaaaagac
      601 acccttcaaa aatatcactc aaagatagaa aacaatatga aaattttgtt atgaaatggg
      661 ttaacaaaga atacataaaa ctattttcca aaaaccaatt ttatcaagcc ttaaaatatg
      721 caagaattga cgatttaaac aatattagct ttgaacaatt cttgtctctt ttcaatagct
      781 ataaattatt taatagataa gttaaggggt gcataaactg catcccttaa cttatcttat
      841 tttgcttaaa caatcttggc ccgtaaactg cataactcat atgtaaatcg ctccttttta
      901 ggtggcacaa atgtgaggca ttttcactat ttccggcaat tacttccatg taaagtataa
      961 cccactatac tttacttcgt aaaggtgggc tcgccgaggg gtcttgcaga cggctctgtc
     1021 ggctcttttg agccgagtat cagcgcaccc ttatggtgca attagctgat acaaaaaaca
     1081 aagagttggc tcatgtaatc caactcttaa aacacaaaaa aataaccccc ttggcttttc
     1141 agcagggggc atatttttta tttaattcct cactctttat ggttctgttg caaagttttc
     1201 caaaaaatct attttagtgt aaaattgaga aaaaagacag agaggacaga gtaatgaatc
     1261 attttaaagg caaacaattc aaaaaagacg tcattattgt cgctgttggt tactacctgc
     1321 gttacaatct aagctatcgt gaagttcagg aattgttata tgatcgtgga ataaatgttt
     1381 gtca
//
LOCUS       DAJSMI010000289         1379 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJSMI010000289 DAJSMI010000000
VERSION     DAJSMI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.294"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(941..1354)
                     /locus_tag="ON430_002809"
     CDS             complement(941..1354)
                     /locus_tag="ON430_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCR3220677.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          509 a          230 c          207 g          433 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtat
//
LOCUS       DAJSMI010000290         1349 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJSMI010000290 DAJSMI010000000
VERSION     DAJSMI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.296"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            14..673
                     /locus_tag="ON430_002810"
     CDS             14..673
                     /locus_tag="ON430_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfX family protein"
                     /protein_id="HCR3220678.1"
                     /translation="MEENNIESKDNLFKEIGEKMYPLFFNRNFGLATKSEIESMLFYS
                     YLKHKINSNSDYSDRTLSRELGISEVKVRNLKRTCYARYEDAIDFPKLLTSLSKEGSN
                     LMFFKIYTDNNDVLCRISISEVVYYDELRTQLNNSGILFKKNPGSNYVELSMADAIIF
                     FDQDSISKESLDLKNLIDKMKQLNFKTNTKMDIDKILEGTKAGRMKNVVEAMMKLSNS
                     I"
     gene            complement(932..1276)
                     /locus_tag="ON430_002811"
     CDS             complement(932..1276)
                     /locus_tag="ON430_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220679.1"
                     /translation="MLEGGIQMKKIVWIYSVNLKGMGLYGNSTTMPLRQAQKFQETIK
                     TNLPSDVTVDFISYDTSSTEIPKADLIVYNDIDSRYLSDDLKNNGIVIPFKDMISNNT
                     REIEKKILLAIK"
BASE COUNT          502 a          179 c          202 g          466 t
ORIGIN      
        1 taattctata cctatggaag aaaataatat agaaagcaaa gacaatctgt ttaaagagat
       61 tggggaaaaa atgtatccgc ttttttttaa tcgtaatttt ggattagcaa caaaaagtga
      121 aattgaaagc atgctctttt actcgtatct aaaacataag ataaatagta atagtgatta
      181 ctcagataga accttgtcta gagagctagg aatatcggaa gttaaagtaa ggaatcttaa
      241 gagaacatgt tatgcaagat atgaagatgc aattgatttt cctaaattac taacctcgct
      301 gtccaaagaa ggctcaaatc taatgttttt taaaatctat actgacaata atgatgtatt
      361 atgtaggata tctatttccg aggtagttta ttatgatgaa cttcgaacac aattaaacaa
      421 tagtggtatt ctttttaaaa aaaatccagg atcaaattat gtagagttat caatggcaga
      481 tgcaataatt ttctttgatc aggattctat cagcaaagaa agcttagatt tgaaaaattt
      541 aatagataag atgaaacagt tgaattttaa aactaatact aaaatggata ttgataaaat
      601 attagaagga actaaagctg gtagaatgaa aaatgtcgtt gaggctatga tgaagctttc
      661 caactcaata taaacaaaga ggtcagaaac ttctaagttt tttgacctat tgctttttcg
      721 tgtgtaactt ttttatttaa atggcataag tttttatcga tgaatggcaa aagctaccaa
      781 aacaaatgga aaaaaccagt tgtatattac aactggtttt tttgaacgaa atctcgttca
      841 atggatagct taactataat ataagtatac aaaatatgca agcgtttaca ttaaataaaa
      901 aattgaattt ggtagtaggg tacatatgat tctatttaat agctaacaaa atttttttct
      961 ctatttcacg tgtattatta gaaatcatat ctttaaacgg gataactata ccattatttt
     1021 ttaagtcatc acttagatat ctggagtcta tgtcattata tacaatcaag tctgccttgg
     1081 gtatttcagt acttgaagta tcatagctta taaaatcaac agtaacatca gacggcaaat
     1141 tcgttttaat tgtttcttga aatttttgtg cttgtctcaa aggcatagtt gtactatttc
     1201 cataaagacc catacccttt aaatttacag aatatatcca tactatcttt ttcatttgta
     1261 ttcccccttc taacatatta tttactttaa atatatcact ttgtaaaatt aatacaagaa
     1321 atatctacaa cgattttaag caatgtgat
//
LOCUS       DAJSMI010000291         1335 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJSMI010000291 DAJSMI010000000
VERSION     DAJSMI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.297"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            6..398
                     /locus_tag="ON430_002812"
     CDS             6..398
                     /locus_tag="ON430_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3220680.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            399..>1335
                     /gene="aac(6')-Ie"
                     /locus_tag="ON430_002813"
     CDS             399..>1335
                     /gene="aac(6')-Ie"
                     /locus_tag="ON430_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HCR3220681.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGL"
BASE COUNT          548 a          108 c          226 g          453 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat aaagatgaaa
      361 tagtatatgt atatgattat gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa
      421 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
      481 aaagagtatt agaattttat ggtggtagag ataaaaaata tacattagaa tcattaaaaa
      541 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
      601 ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt
      661 atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt
      721 ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa
      781 gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat
      841 accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta cacgagggca
      901 aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg
      961 caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa
     1021 tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa
     1081 aatttagtac taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa
     1141 atacaaattt agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg
     1201 aattatctat actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt
     1261 ctactatgtc agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac
     1321 aaatgcacgg tttag
//
LOCUS       DAJSMI010000292         1327 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJSMI010000292 DAJSMI010000000
VERSION     DAJSMI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.298"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..669
                     /locus_tag="ON430_002814"
     CDS             <1..669
                     /locus_tag="ON430_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220682.1"
                     /translation="KSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKII
                     RFGILGIALIACLVSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHELF
                     GVGIPNNHDARAVEYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALAI
                     SIIDAGFRRDKSVAAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDEF
                     IYIE"
     gene            686..>1327
                     /locus_tag="ON430_002815"
     CDS             686..>1327
                     /locus_tag="ON430_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220683.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKS"
BASE COUNT          455 a          184 c          267 g          421 t
ORIGIN      
        1 aaaagtatat ggaattgcat ttttagttta ctgacatttg ttataatctc gcaaggcaga
       61 agtggtatat tatatgtagt aataacctcg gttattatgc ttttaatttc gcttaaacca
      121 tccaagatta ttagatttgg tatattagga attgccttaa ttgcatgtct agtgtctgtg
      181 acccaaatta gagaggtcgt tatatcttcg tttgtagagt ctcagagcag tgacacaggt
      241 aatatcgcat atagaattgc tgaacaagat tattttgaaa ataaattaga aggtcatgaa
      301 ctatttggag ttggtatacc aaacaatcat gatgccagag ctgtagaata ttcaggcaaa
      361 acaatagatt ttgagtctaa aaaatataat ccctattatc ttgaggactt aggcgctttt
      421 gaaattcgtt atagatttgg tataattgca tatgcgttat atatagttgc tttagctata
      481 agtattatag atgccggatt tcgaagagac aaatcggtcg ccgcatttgc gggtatggca
      541 ttcatggtgt ttatggcgct taattttaat ttgctagcgt atattacagc aagaccgttc
      601 ggatttatga tttctttaat tttgatagaa atatcggcat tcaatgatga attcatatat
      661 attgaatagg taagggagag cacagatgaa tttagatcga ttaaagaaaa tagcaaatat
      721 gatttcacca atcataaaag tgataaatgc gccgttagcg tattttagtg ggaaatcaga
      781 ttatatggag aagggaatat tgtggcctac tgtaacaacc tactccaata acagtagaaa
      841 agtgattcaa tatacaactt tacctaaaaa cgtagacaac atggctcaaa tgcaatgggc
      901 tggggaaagt tttgaggaca tatgtatagt tcttcaaggt ccagtattag aagacggtga
      961 ttttacacta aataccgtta aaatgtataa taaatactat cctaaagtaa aacttgtagt
     1021 gtctacttgg ataggattat ctgaagagac agaatgcaag ttgcgtgaag ctggtgcaga
     1081 tgttgtatta tgcaagaaac ctgaaaaaac aggtgcgtac aatattaatc tacaacttac
     1141 ttctgcatac gaaggcgtaa aaatggcgca aaaactgggg tgtagttatg cgctgaaaac
     1201 gcgaactgat caaagactat atgcaaatga tgtactaaag tatttccaaa cattattgaa
     1261 tatttttcca tcttcggact gtaaagtagt gaatcagcga ttaatattta cgacttatgg
     1321 aaaatca
//
LOCUS       DAJSMI010000293         1326 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJSMI010000293 DAJSMI010000000
VERSION     DAJSMI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.299"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            82..>1326
                     /locus_tag="ON430_002816"
     CDS             82..>1326
                     /locus_tag="ON430_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCR3220684.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFE"
BASE COUNT          449 a          258 c          253 g          366 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgag
//
LOCUS       DAJSMI010000294         1319 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJSMI010000294 DAJSMI010000000
VERSION     DAJSMI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.300"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..134
                     /locus_tag="ON430_002817"
     CDS             <1..134
                     /locus_tag="ON430_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3220685.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            131..1009
                     /locus_tag="ON430_002818"
     CDS             131..1009
                     /locus_tag="ON430_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220686.1"
                     /translation="MTNHKRKNCVIPLAISMSLLIYTHVLSTFMSVIVIGFLFIGSLF
                     SKRKEKKEMSDLILNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTNLQERALNI
                     SDSLIGAVNNDLVTFTMGTIGVVSLILPLFFLKKFTNKEKIIYFCAVTTWFLSTNLFP
                     WEILQKTPIQIIQFPWRILGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTV
                     TAATEANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVK
                     VGDRWRMHLIKHIIPK"
     gene            970..1266
                     /locus_tag="ON430_002819"
     CDS             970..1266
                     /locus_tag="ON430_002819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220687.1"
                     /translation="MENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKS
                     TIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCG"
BASE COUNT          458 a          192 c          222 g          447 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc ataaaagaaa gaactgtgtg attcctttag ctatcagtat
      181 gagtttgcta atctataccc atgttctctc cacttttatg agtgtgattg ttattggctt
      241 cttgtttatc ggctctcttt tctctaagcg aaaagagaaa aaagaaatgt cggatctgat
      301 attaaatatg ataaaagctg ttggttggac cattctttta acggcttacg tttggtatcc
      361 gatgttacaa caagtcctac atcaaaaaat taatcgtcct tttcgcacaa atcttcaaga
      421 aagggcattg aatatttctg atagtttaat cggtgcagta aataatgatt tagtcacgtt
      481 tacaatggga acaattggtg tggtttcatt gattcttcca cttttctttt taaaaaaatt
      541 taccaataaa gaaaagatta tttatttttg tgcagtgacc acttggtttt tatcaacgaa
      601 tctttttcca tgggaaattt tacaaaaaac acctattcaa atcattcaat ttccgtggag
      661 aatcttgggt tttcaagtgc tttttggttc attaatctta accatagtgt ttttaaagtg
      721 gaaaacaagc aacaaaaaaa gtatgtggag ccttgtagga atagttctta ttatttttac
      781 tgtgactgca gcaacagaag caaattattc gcataaaatt cagtcgtata aaggtcgttt
      841 agttatgacc aaaaaagatg ttacacgtta tacatcctca agaactggag gcctatatga
      901 ctatgcacca ttagatgcat tgaaatataa agatcattta aagaaacatg aagtaaaagt
      961 tggtgatcga tggagaatgc atctcataaa gcatataatt ccaaaatagt ctatacagta
     1021 gaagatgccg atgagcaaaa tatcacatta cctgtattca attattgggg aacagttgtg
     1081 aaagtaaatg ggaaaaagag cactatagaa aatgacaatg gtttagtgtc tattaaaggt
     1141 acgggaaaaa gaactatagt tgaggtttca tctacgtatc caactacgat gattctatct
     1201 ttattaatta gttcaataac ttttatattc ttatttacac gttatttaat aagatgctgc
     1261 ggttaaatga aaatagatta gatagatatt ataaatatgt tgattctatt ttcatagca
//
LOCUS       DAJSMI010000295         1316 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJSMI010000295 DAJSMI010000000
VERSION     DAJSMI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.301"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            2..400
                     /locus_tag="ON430_002820"
     CDS             2..400
                     /locus_tag="ON430_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR3220688.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="ON430_002821"
     CDS             384..1079
                     /locus_tag="ON430_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR3220689.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          498 a          197 c          263 g          358 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgttt
//
LOCUS       DAJSMI010000296         1314 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJSMI010000296 DAJSMI010000000
VERSION     DAJSMI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.302"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..96
                     /locus_tag="ON430_002822"
     CDS             <1..96
                     /locus_tag="ON430_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HCR3220690.1"
                     /translation="PKKGEKPLLTLFTCVYTSQGKERYVVQGELQ"
     gene            149..>1314
                     /locus_tag="ON430_002823"
     CDS             149..>1314
                     /locus_tag="ON430_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR3220691.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWG"
BASE COUNT          485 a          208 c          297 g          324 t
ORIGIN      
        1 ccaaagaaag gagagaaacc cttattaacg ttgtttactt gtgtttatac gtcacaagga
       61 aaagaaagat acgtggtaca gggagagcta caataacaag gttttaatca acgcgagtcg
      121 ttgaaatatg aaatgaggag agtaaaaaat ggcaataaat cggtatgtaa aaaaagctgg
      181 aatgcttgtt ggaatgttag gaatcattgc aggctgcttt caggggtatc aagtacatgc
      241 agaagatgtc acgcaaaaaa caccaccaga gaaggtcaat atcacggttc ataaattaat
      301 gtacgatcaa ggaacgcaat tgaatgttga tattgacggg atcaaaaacg atggttatac
      361 gcacgatcaa tatcctacag gtgtaacgaa atacaacaag gcagattatg gtgatgttga
      421 gtttacgtta ggaaatatta cggatcaagt attaccgaga gaagattctg atctaaccaa
      481 cgcgaaagtg gacgagattg tacaagatgt agaagacaag ggatctaaca gtgagtacgt
      541 caagaatgca accaacaaaa ttacttcagc tgtagatgaa aatggtgaga tcacttttac
      601 tgatcaacca gcctatgtca attccaaagg gaatgtatat gtggtttatg aaagtaaatc
      661 cgctgctgga ttagtcacac aaaaagcaaa accaatggtt gtgattgcgc caatgacaga
      721 taaaacagga tcaggatttt taaaaaatat tcatttgtat cctaagaaca ttgtttctaa
      781 gttgagtttt gaattaacga aatttggtga cgatggcgca gaccaaagca aacaaaccct
      841 tttaaaaggg gcaaaatttg agttatacaa gggcgaacct ggaaaaggaa caaaattggg
      901 tgatcttgta tcggatgatc aaggaaagct gactgcgact gatttgacgt taggaaaata
      961 ctattttgtg gaagttcctt cggaagttgt agtgggatca gatcaagaac caacagcaga
     1021 tcaatattta ttaggcgcag acgctcgcaa cgatgcgcac aataaattaa cctttgaaat
     1081 tacgaatgat ggcgtgacaa gtgacttaaa agcttcatac gtaaactata aagcaccagt
     1141 gatcgataaa acagtaacaa atggaacagg acaagaacat tctttccaaa ttggtgacgc
     1201 agtaaattat caaggaacga ttcatattcc aacagacatt gcaggtggcg cagacggtat
     1261 cacagtaaac ggtgtgaaaa gcgaaacttc tccttacagt gtatttaaat gggg
//
LOCUS       DAJSMI010000297         1311 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJSMI010000297 DAJSMI010000000
VERSION     DAJSMI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.303"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            129..>1311
                     /locus_tag="ON430_002824"
     CDS             129..>1311
                     /locus_tag="ON430_002824"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3220692.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LH"
BASE COUNT          411 a          262 c          236 g          402 t
ORIGIN      
        1 gctctataat ttctaggact gatggaaatt ttctatctgt cctagttttt ttgtaaaaaa
       61 acaaaaagac atacaaaatt tccggtagaa tagagtctcc tacaacatca ttctggggga
      121 attcttgtat gtccatgaat cattttatca aacaactctt tactattaaa gactcaaata
      181 tccaaattca atcctttgaa gaaacttttt ataaaagcag aaaaagtatc gttattgagg
      241 gaactctgat ccgtacctac agacgttgtc cgtgttgtaa agattctaca aagcaaatcg
      301 ttaaaaatgg caagaagatc tctatgattc tcctgaatcg tagcggtaat aaacggacct
      361 atcttcggtt gaagaaacag cgatacctct gccgagcatg taaaaaatat tttaccgctc
      421 ggacgtatct cgttacccca ttctgcttta tttctaagca gattcattac aagatcttag
      481 aagaattgac agagcgtcaa tctataaaag caattggtaa acattgcgat gtttctgtga
      541 caactgttca gcggacattg tccgccttgt catcagatat ccgtcagaat cttgagtatt
      601 tacctcggtg tctattattc gatgagtttc gttctctttc tacatggcac ggtaaatttt
      661 ctttttcttg tatggacggt gagaccggga aactgtttga gatcttacct agccgccgta
      721 aaaaagatct cgttgctttt ttcatgcgtt tttctctcaa agcgaggttg ggtgtacatt
      781 acatcgtaac ggatatgaac gctccttatt tctcactagt taaagagtgt tttccaaatg
      841 cacaagtgat cgtcgatcgg tttcacattg ttcaacatct gaaccgcttc ttcgatgcgg
      901 ttcgaaaacg tgtgatgaaa acacttgatc aaaaagaccc aatacaagct aaacagtatc
      961 gccaattgaa atccttattc aaattacttt taaaatcaga ggatcgttta gattacaata
     1021 cactaacgaa aagacgcaat ttcaattgga gactattgac tgaaacagaa gtagttgacc
     1081 gccttctaaa gatttctcct gaacttaaaa cagcttatcg ttactaccaa gacctgctta
     1141 ctgcgtatca ggaaaaagat ccagatactt ttttccagct gcttcgagca atgcctggag
     1201 aagttccatt ggaactgcac catatcaaaa aagccttttt caaatacgag aaagggattc
     1261 ggctggcatt actgaagaag tattcgaatg cacgactgga aaatctccat a
//
LOCUS       DAJSMI010000298         1300 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJSMI010000298 DAJSMI010000000
VERSION     DAJSMI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.304"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..264
                     /locus_tag="ON430_002825"
     CDS             <1..264
                     /locus_tag="ON430_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR3220693.1"
                     /translation="VNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGII
                     KMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            269..1216
                     /locus_tag="ON430_002826"
     CDS             269..1216
                     /locus_tag="ON430_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR3220694.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          429 a          188 c          268 g          415 t
ORIGIN      
        1 gttaataaat caagatacac atctaggcaa cagacacctg aagtttttga aatgaatgcc
       61 tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta attatatttt tgatgggcaa
      121 tgtgggataa taaaaatgag agattatcta attttagata ttgatagtga agaagatttt
      181 aaatggttag aatttttgtt tcctaaattt ttagaagatt cttcggagct tcaagatatc
      241 tataattatt tatatcggag gtaaaaggat gagctgttat cttatcaccg gtggggctgg
      301 cttcattggt tctaccttag ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga
      361 tgacttatca atgggaaaag tagaaaatct tgatatgacg aaaaatatca cttttattga
      421 gggagatgtc gctgagaaat ccttgatgga agagatcatg aaagctaata aatttgatta
      481 tatctttcat ttagcagcag ttgctagtgt ggcagattca gttgcccgac cagtagaaac
      541 acatcgtgtg aattttgaaa gtgtattaat gattttagaa ttgatccgca agtatcaacc
      601 tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt tatggggatg aaccgacttt
      661 accaaaaaaa gaagagtccg tgattcgccc attgacgcct tatgcggttg ataaattcgc
      721 tgcagaacaa tatgtgctag attattgtca tttatatgat gtacccggta gcgcagttcg
      781 ttttttcaat gtctatggtc caaatcaaaa tccgaattct ccatacagtg gtgtgatttc
      841 tattctcgta gatcgctata agaaacaaat cgtaggagaa gcaacttcat ttacattatt
      901 tggtgatgga agccagtcta gagactttgt ttatatagat gatgtaatcc aagcattact
      961 tttagttgcg aatgaggaag ctgcactggg gaaacaattc aatgttggaa cgggtaaagc
     1021 aaccacactt ttggaattga ttcatgcaat caatgaaatt ctcggtgtag aattgacact
     1081 tgaatacaaa gaagagcgtt cgggtgatat ccatgactct ctagcagata ttactaagat
     1141 ccagtcaatt ggctatcaac cgaaatatgg tattttaagt ggaatgacta attatttgaa
     1201 aactgaaatc aattaaaaca tactaagata agattagagg gtaaattcag gctctttgtc
     1261 aataaggact gatgagtttt gtaaaatcaa atctgggata
//
LOCUS       DAJSMI010000299         1290 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJSMI010000299 DAJSMI010000000
VERSION     DAJSMI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.305"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>1290
                     /locus_tag="ON430_002827"
     rRNA            <1..>1290
                     /locus_tag="ON430_002827"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          342 a          287 c          393 g          268 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta
//
LOCUS       DAJSMI010000300         1251 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJSMI010000300 DAJSMI010000000
VERSION     DAJSMI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.307"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            143..258
                     /gene="rrf"
                     /locus_tag="ON430_002828"
     rRNA            143..258
                     /gene="rrf"
                     /locus_tag="ON430_002828"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            272..344
                     /locus_tag="ON430_002829"
     tRNA            272..344
                     /locus_tag="ON430_002829"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:305..307,aa:Val,seq:tac)
     gene            348..420
                     /locus_tag="ON430_002830"
     tRNA            348..420
                     /locus_tag="ON430_002830"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:381..383,aa:Lys,seq:ttt)
     gene            428..509
                     /locus_tag="ON430_002831"
     tRNA            428..509
                     /locus_tag="ON430_002831"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:462..464,aa:Leu,seq:tag)
     gene            527..599
                     /locus_tag="ON430_002832"
     tRNA            527..599
                     /locus_tag="ON430_002832"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:560..562,aa:Thr,seq:tgt)
     gene            611..682
                     /locus_tag="ON430_002833"
     tRNA            611..682
                     /locus_tag="ON430_002833"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:643..645,aa:Gly,seq:gcc)
     gene            699..784
                     /locus_tag="ON430_002834"
     tRNA            699..784
                     /locus_tag="ON430_002834"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:733..735,aa:Leu,seq:taa)
     gene            795..868
                     /locus_tag="ON430_002835"
     tRNA            795..868
                     /locus_tag="ON430_002835"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:829..831,aa:Arg,seq:acg)
     gene            878..951
                     /locus_tag="ON430_002836"
     tRNA            878..951
                     /locus_tag="ON430_002836"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:912..914,aa:Pro,seq:tgg)
     gene            971..1043
                     /locus_tag="ON430_002837"
     tRNA            971..1043
                     /locus_tag="ON430_002837"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1004..1006,aa:Ala,seq:tgc)
     gene            1071..1144
                     /locus_tag="ON430_002838"
     tRNA            1071..1144
                     /locus_tag="ON430_002838"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1105..1107,aa:Met,seq:cat)
     gene            1158..1231
                     /locus_tag="ON430_002839"
     tRNA            1158..1231
                     /locus_tag="ON430_002839"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1192..1194,aa:Ile,seq:cat)
BASE COUNT          301 a          283 c          347 g          320 t
ORIGIN      
        1 agtgatgtat ggagcggact gatactaatc gatcgaggac ttaaccaata ggttaaaaga
       61 aaagaactcg gaaaaaaact tatgtgactt caaatccagt tttgagtgaa caaaatttac
      121 tcaataaaag ataacaccac agtgtggtgg cgatagcgag aaggatacac ctgtaaccat
      181 gccgaacaca gaagttaagc ttcttagcgc cgattgtagt gaggggttgc cccttgtgag
      241 agtaggacgt cgccacgctt aagttatcca tggaggttta gctcagctgg gagagcatct
      301 gccttacaag cagagggtca gcggttcgat cccgttaacc tccatatgag ccgttagctc
      361 agttggtaga gcatctgact tttaatcaga gggtcacagg ttcgagccct gtacggctca
      421 tatttttgcg ggtgtggcgg aattggcaga cgcactagat ttaggatcta gcgccttacg
      481 gcgtgggggt tcaagtccct tcacccgcat aatttaagtt ttacatgccg gcttagctca
      541 gttggtagag catctgattt gtaatcagag ggtcgagggt tcaagtcctt tagccggcat
      601 ctttaaacac gcggaaatag ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg
      661 ggttcgaacc ccgttttccg ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag
      721 acgcacagga cttaaaatcc tgcggtgagt gatcaccgta ccggttcgat tccggtcctc
      781 ggcatttcaa atatgcgccc atagctcaac tggatagagt gtttgactac ggatcaaaag
      841 gttaggggtt cgactcctct tgggcgcgtt aattaaacgg gaagtagctc agcttggtag
      901 agcacttggt ttgggaccaa ggggtcgcag gttcgaatcc tgtcttcccg ataattataa
      961 atttaaacac ggggccttag ctcagctggg agagcgcctg ctttgcacgc aggaggtcag
     1021 cggttcgatc ccgctaggct ccatttcatt taaataatga atatcttttt cgcggtgtag
     1081 ctcagctggc tagagcgtcc ggttcatacc cgggaggtcg ggggttcgat cccctccgcc
     1141 gcgattttta agagcctgga cctttagctc agttggttag agcagacggc tcataaccgt
     1201 ccggtcgtag gttcgagtcc tacaaggtcc attgtggcgt tgtaagtttc a
//
LOCUS       DAJSMI010000301         1239 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJSMI010000301 DAJSMI010000000
VERSION     DAJSMI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.308"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>1239
                     /locus_tag="ON430_002840"
     CDS             <1..>1239
                     /locus_tag="ON430_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR3220695.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSR"
BASE COUNT          406 a          221 c          268 g          344 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgta
//
LOCUS       DAJSMI010000302         1220 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJSMI010000302 DAJSMI010000000
VERSION     DAJSMI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.309"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..309
                     /locus_tag="ON430_002841"
     CDS             <1..309
                     /locus_tag="ON430_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HCR3220696.1"
                     /translation="THTIKVSFQRWDYKGFVTFRRGGNCKGLDVLALDEDDLYYQKLT
                     DNPIGFGLLPEDDEGNEWFKMTLTNDNGDELSVEDTWSYLSDYIVSVEIIEFVADKEE
                     "
     gene            423..662
                     /locus_tag="ON430_002842"
     CDS             423..662
                     /locus_tag="ON430_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220697.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            774..968
                     /locus_tag="ON430_002843"
     CDS             774..968
                     /locus_tag="ON430_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220698.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKKSSNCQLLLEVS"
     gene            1054..>1220
                     /locus_tag="ON430_002844"
     CDS             1054..>1220
                     /locus_tag="ON430_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3220699.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKE"
BASE COUNT          467 a          139 c          269 g          345 t
ORIGIN      
        1 acacatacta ttaaggtttc tttccaacgt tgggattaca aaggatttgt gacgtttaga
       61 agaggcggaa attgtaaagg attagatgtt ttagcattag acgaggatga tttatactat
      121 caaaaattga ctgataatcc aattggattc ggcttgctac ctgaagatga tgaaggtaat
      181 gaatggttta aaatgacgct tacgaatgat aatggagacg aattatctgt tgaagatact
      241 tggtcatatt tgagtgatta tattgtcagc gttgaaatca ttgaatttgt agcggataaa
      301 gaagaataaa aactggagga ataaatttta cagataaagg cttagaagaa gtcacgacga
      361 ataataaaaa ggtagtatta ccttaggcat tgagtaagga attcatttag gaggaaaatt
      421 agatggcgcg accagtttta gaggcgatgg atatggatac tttgacaaaa ggaaggtatg
      481 tacagctgtt agctcgtggt tattcaccta aagatgtatt caaatccctg tctaaaggaa
      541 cagatatgga aaaagaaaga ttacgaaaag aattcgatta ttggagaacc cataatggaa
      601 tcaaggattt gaaaccagca cggccagtaa ttaaacgaaa gaaaaagata acccaagact
      661 gaagtgtaga gcgtatgtga tcataggctt ttttattttt gcaaaaaaca aggaaggtag
      721 gaaaactcat gaattatgaa gaaatcgaaa acagaaaaaa agtgtcgaaa taaatggaag
      781 aaaagttgct aaaaacgatg aaacaaaaac accttaaaag attatctgtc atgcaatata
      841 tcaatgatat gcagataaca ggaaaagaga aagcttgttt gttaggttca atgaaaaact
      901 ttgaacagtt gagacgaact tatgtgaaaa aaagttctaa ttgtcaacta ttgttagaag
      961 tctcatgaca ttcattaata gaattcattt ttaaaacttt ttctatggaa atgagaaaaa
     1021 gattttttgt tttgtctgaa agggggtagc tggatgagaa aatggttatt tggtttttta
     1081 ggagtcgctt taatcgtctt ttgcagtgtg tttggctatg ttagctacca aaaacatgaa
     1141 ggagaggtgt ttaagcaaaa tatagaaaaa aagatgcccg tagatcaaat caatgcacat
     1201 gcaaagagtt ataaggagga
//
LOCUS       DAJSMI010000303         1205 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJSMI010000303 DAJSMI010000000
VERSION     DAJSMI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.310"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            414..947
                     /locus_tag="ON430_002845"
     CDS             414..947
                     /locus_tag="ON430_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR3220700.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          387 a          222 c          224 g          372 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggac
//
LOCUS       DAJSMI010000304         1185 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJSMI010000304 DAJSMI010000000
VERSION     DAJSMI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.311"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(17..1057)
                     /locus_tag="ON430_002846"
     CDS             complement(17..1057)
                     /locus_tag="ON430_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3220701.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT          337 a          193 c          171 g          484 t
ORIGIN      
        1 atttgtcagt gaaatttcac tctaaccagt ctttcgtaat agtttttggg aagctttcag
       61 aattcaaacg tttttcactt gatactttag cagctattgg tttttcgata aaccaatttt
      121 ttacatagac aaaaatcagg ttctggatat tttcaacttt ttcggttttt aatttttgat
      181 aggcttttaa cagtgtcgtg tataattctt gttctgcatt gacataataa ctatcgagtt
      241 cttcaaaaat tattgaaata ccactttctg tttcggcttt tttctttgca ttatgaatag
      301 tctggacagt tttttgagct tcactgtaac ttgaagaaaa tgtttttatt aaatttaata
      361 ctcgttcagg gataaacgta gcaatgctgt cgtttgtcat aattgtctca aaattgtcta
      421 acaacaattg atcttgtagg cggtcttttt cagtgtctat attgtgtcta ttagtgtcta
      481 atatattatt attaagttct ggtatgattt tcataccctc tgatttatca acgttttggc
      541 acggtatgat tttcataccc tcttgagccc caagggattc agcgtctgat ttttcgtcga
      601 gggtgggtat gattttcata ccctctgatt tatcaacgtt ttccctattc tctctatcaa
      661 atttttcgag taagtagata tcattttctg agacttttaa ttctgctaga tatagtctat
      721 taggattatt tttccctaac tgtttgttaa atcccatctg cttttgtaat aataaaccaa
      781 aggattccaa ctcttttttg attgctatcg ctttttgttt ttggcagttt aatacgctac
      841 atagttcatt tgtcgtataa gtaaaataaa cattaccttc ttcatcgatt tgccgtttgc
      901 tgattgcgat ctctaatctt gcttttaaaa gcatataggc aatcttagtt gaatctttca
      961 tttttttgta ttcgtcagaa acaataaaga cttttggaaa ttgataaaat aattcgttgt
     1021 aaactctatc cgccttaaaa aaattaaaat cagtcattgt agatcctcta ctttcacatt
     1081 gttttattta tgtaaaatta cctactcttt tcgaaaatag caaagataaa taaaaaatgc
     1141 aaacgcatta aaactgtttt ttaattaaat actacctaaa atcag
//
LOCUS       DAJSMI010000305         1178 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJSMI010000305 DAJSMI010000000
VERSION     DAJSMI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.312"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            108..458
                     /locus_tag="ON430_002847"
     CDS             108..458
                     /locus_tag="ON430_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220702.1"
                     /translation="MSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSDKFRNYI
                     ADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIG
                     IFKKNTSEEKNLKL"
     gene            614..988
                     /locus_tag="ON430_002848"
     CDS             614..988
                     /locus_tag="ON430_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCR3220703.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDVEWQHFF"
BASE COUNT          457 a          154 c          239 g          328 t
ORIGIN      
        1 gcctattact tgcattatgt ttgagcttaa gcgcagttgg gtgtcaaaaa agtaatgaaa
       61 acaataaaat aaaagaagga caatccataa gttctgaaga ggaaggcatg tcaattaatg
      121 caaatggcaa aaatgaaact tttaagccat cagattatac attggaggca aagaaagaat
      181 atgtttatga atatttaggg cttaaattta aactctcaga caaatttaga aattatatag
      241 ctgataaaaa gatagcgatg ttagatgatc aaagtcctat agataaagaa ctaaagtatg
      301 ctattttgac atttgagaaa atgacagaag aacaaaagaa tgcagttata gaaaagatgg
      361 gcgatggata taaaaattgg caaaatgagc ttgaaagaat tgggactatt ggtatattta
      421 aaaaaaatac atcagaagaa aaaaatctaa aattataaaa tgtgatactc atactaaaat
      481 aggagtttca agtgatggaa aatacgattg ctatttaagc acaaatagcg gagctgaaag
      541 taatcttctg gatgaattaa aaagaactga gattcaaatt atagataaaa aagaacgtcc
      601 taaaaatggt tttgtgctat cagaaaaaac tgatttagaa aatacagaag catttaacaa
      661 agaatcggtt aaagatttac gtaaactacc aacaaaggac ataaatggca aggattttac
      721 aagtaaagat tttgagaagt atgatcttac tatggtaaat gtatttgcaa cttggtgtac
      781 agcttgtgta aaggaaattc ctgatttggt tgaggttcag aatgaaatga aaagcaaggg
      841 tgtaaatatc gttggagttg ttacagatcc tgttgatgat aacggtgaaa acaaggaagc
      901 aattgaaaag tcaaagttaa tacatgaaaa gacaaaggct tcatatcctt tcttaatgcc
      961 agatgtggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1021 gttatggctc cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1081 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1141 attcgcttcg ccaatctctg ttgtcgaatt tggatcgg
//
LOCUS       DAJSMI010000306         1178 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJSMI010000306 DAJSMI010000000
VERSION     DAJSMI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.313"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..693
                     /locus_tag="ON430_002849"
     CDS             <1..693
                     /locus_tag="ON430_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3220704.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
     gene            complement(967..>1178)
                     /locus_tag="ON430_002850"
     CDS             complement(967..>1178)
                     /locus_tag="ON430_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220705.1"
                     /translation="VNIVIFTDWLGVLFSNLIPILIIGICGIILSILELFESRNTMNR
                     IFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          439 a          180 c          200 g          359 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct cccacttcaa gcgttaagaa ccgtcaggtt tagctaagtg
      841 gtgagtagtt cacatctgat aatatattga gtgttccaat gagggctttg atcctattat
      901 ccatatcttt ttcagtttta atattgatac acattataag aaaaagaaac ttttggttaa
      961 tttatttcat cccagtgcaa aatataaaaa agtaaaatag accataggca atagattaac
     1021 aattaatata atacaagcaa atattctatt cattgtattt ctgctttcaa aaagttctaa
     1081 tattgaaagt ataattccac aaatgcctat aataagtatg ggaataagat tagaaaataa
     1141 caccccgagc caatctgtga aaattactat atttacta
//
LOCUS       DAJSMI010000307         1120 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJSMI010000307 DAJSMI010000000
VERSION     DAJSMI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.314"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..1107
                     /locus_tag="ON430_002851"
     CDS             <1..1107
                     /locus_tag="ON430_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3220706.1"
                     /translation="VDKIDIQNKKISCVKKDGTRFSQEYDKVILATGSKQIMLDIPGN
                     NLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVA
                     DRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMV
                     VQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVD
                     FSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSESID
                     YIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQI
                     TLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT          431 a          136 c          189 g          364 t
ORIGIN      
        1 gttgacaaaa ttgatattca aaataaaaag attagttgtg taaaaaaaga tggtacaaga
       61 ttctctcaag aatatgacaa agttatatta gctacaggtt caaagcaaat tatgctagat
      121 ataccgggga ataatttagc taatattttt tcgataaaga attttccaaa tgcaaaagag
      181 atttttaaag ctttggatga tagtactatt aaaaaagtag gaattattgg agcaggatat
      241 attggagttg aaattgctga agccataaga aaaagaggga aagaagtata tcttttcgat
      301 gttgcagata gagtgttgtc gacttattac gaccgtagtt tttcagataa agttgaagaa
      361 atactatcaa aaaatggaat acatctttgt ctatcagaaa gtcctaaaaa atatttagga
      421 agaaaaaaga ttgaaaaaat agtcactaca agtggtaccc aatactctat ggatatggtc
      481 gtacaagcta taggatttct atctaatagt ccaattgggg aaaaggaact cttgagagat
      541 gttagtggag catatctaac taatgaatac cagcaaacaa atataaaaga tatatatgct
      601 attggggatt gtgctacaac tttttttacg tccattaata gaagtagtgt tgatttttct
      661 atttctaatg ctctaagaac agcttatatt gcaactcaac atattaacaa tcaagatttt
      721 acaccttctg gttcccaatt tacaaatggc ttttctatat ttaattacaa tttttacgca
      781 gcaggtttga atttaaaaac agcacgatta ttaagtgagt ctatagatta tatagaaata
      841 gaagatttca ttagacctgc ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc
      901 tttgaaaaaa atacaaagag aatagtaggt gtacaacttt gtagtcaaga agatttatct
      961 ggactattat caatgttttc tcttgctata gaagaacaaa ttacattaga caaattgaaa
     1021 atgttagatt atttatttca tcctagtttt agccaaccct ataattttat tttaaaggca
     1081 gtaacaggat taaacattga aacataatcg ttactaaatg
//
LOCUS       DAJSMI010000308         1114 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJSMI010000308 DAJSMI010000000
VERSION     DAJSMI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.315"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            56..697
                     /locus_tag="ON430_002852"
                     /pseudo
     CDS             56..697
                     /locus_tag="ON430_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            690..1040
                     /locus_tag="ON430_002853"
     CDS             690..1040
                     /locus_tag="ON430_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220707.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
BASE COUNT          405 a          173 c          235 g          301 t
ORIGIN      
        1 ttttctatta ttctacctta tctgatagta gatttaaaac tttgcaacag aaccccttgc
       61 taactggaat ccttatacga ttaagaatag attaggtgtg attggattac agctgtactt
      121 ccatgctaac ggaatcgatc gaacagatat agcaatcccg ccagaaccga caaaggaaaa
      181 atcttatggt aattcacaag tgttaccacg agattatact agaagaaatg aaatcgagct
      241 ggtggtaaaa gaaatggcgg aacaagtagc cattcgtatc aggcagcata attgtaaaac
      301 aggatgtgtt catttaaaca ttggtacgtc gatcttagaa acgagaccag gattttcaca
      361 ccagatgaaa atacctatca cagataatac aaaggaattg cagaattatt gtcttttctt
      421 gtttgataag tattatgaag gacaagaagt aaggcacgtt ggaataacct atagcaagct
      481 tgtgtacacc gattcgcttc agctggattt atttagtgat ccacaaaagc aaattaacga
      541 agaaaattta gataaaatta ttgacaagat tcggcaaaaa tatggtttta cgtcgattgt
      601 acatgcttct tcaatgttag aaagcgctag aagcattact agaagcacac ttgttggtgg
      661 tcatgcgggc ggaaatgggg gaattaaaaa tgattgatta caatgatcgt ggacgtttaa
      721 agtgggcagg gttttattta tctgatcata ctgaagaaat tgatgccgac aatgaacaac
      781 gaaccagtca aaatttagca aaagaacaaa tgacgactga agagattgcg gaagtattga
      841 acgctgcagt cataaaaaat agatcagtat ctattcaaaa agaagaacga aacgctgaag
      901 gatactatcc agaagatatt gttggcaaga tcttaggtag tgatgagctg gggatctacg
      961 ttggaaaaga caaaattcac tacgacgaaa tacgtcatat cgaattttac caggataata
     1021 aatggagtcg attagaatga acattatcca gcaatatgag ctaaagtata taacctttga
     1081 tcagctatca gaagagattt ggggttatgg ccaa
//
LOCUS       DAJSMI010000309         1106 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJSMI010000309 DAJSMI010000000
VERSION     DAJSMI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.316"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            1..999
                     /locus_tag="ON430_002854"
                     /pseudo
     CDS             1..999
                     /locus_tag="ON430_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          391 a          199 c          222 g          294 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg agacaccggt tggttaccgt aaccagagat tggcgcagcg aatccttgat
      961 aatgagctcg gatgtgctaa cgctagcgag gcagtctaac tttaactgtt agctcctgcc
     1021 gaagatcgtc acatccgagg aggctcattg tcaaggacaa tcggaatagc atacggaaag
     1081 aagttctgca ccttataaaa tttgtc
//
LOCUS       DAJSMI010000310         1101 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJSMI010000310 DAJSMI010000000
VERSION     DAJSMI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.317"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            161..778
                     /locus_tag="ON430_002855"
     CDS             161..778
                     /locus_tag="ON430_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3220708.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
BASE COUNT          385 a          160 c          220 g          336 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaaatgatc cggatttcag aattttaaag gagttttatt atggcgaaaa ttgggtatgc
      181 tcgagtaagc agcttagatc aaaatttaga ccgacaatta gaattattat cagagtgtga
      241 taaaattttt agtgataaag caagtgggaa agataccaat cgagaaggat ttcaagaaat
      301 gatgaaattc atacgtgaag gtgatattgt cgttgtgaca gaattaaaac gacttggacg
      361 aaataataaa gagctaacag aaacgatgaa tttaattcag ctgaagaaag ctacactgga
      421 agttttgaat ttgccagcgt taacaggtat cactgatgat aatttacgtc gattacttaa
      481 taacttaatt atcgaactat acaagtacca ggccgaagaa gaacgtcggt atattcgcga
      541 aacgcaacaa caaggaattg cactcgctaa agcaaaaggg aaatacaagg gaggtacgct
      601 aaaatatcgt gagaatgatc cgcgacttca attagcattt aatttgtttt tgactggatc
      661 tacagataaa gaagttgaag agctaacagg gattaatcgt agaacgttta gaagatatcg
      721 aatgaaacat ggtattacta atgaagttag ggagcaaaaa aataaatctt tcagttgagg
      781 ctaatataat tttgcaagaa accaggtctt aagaaagctc gtaaagcttc acaattctca
      841 aaacgttaat cgttgttata tcaaggattt cacggcatca tccgtttgga tggtgtcttt
      901 tttgggttga attccttgaa aattgggtct attttacaga cccttttcta tataaactgt
      961 tttcgatact tggaaaaatc tgttgttttt aaattttatt taattctaat gtttactatg
     1021 tttttagcag tgttaggtaa tgtaactgca ataaatttgt tatacaataa tggattttga
     1081 ttttgggacg taaaacattg t
//
LOCUS       DAJSMI010000311         1087 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJSMI010000311 DAJSMI010000000
VERSION     DAJSMI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.318"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..275
                     /locus_tag="ON430_002856"
     CDS             <1..275
                     /locus_tag="ON430_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3220709.1"
                     /translation="NPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            618..874
                     /locus_tag="ON430_002857"
                     /pseudo
     CDS             618..874
                     /locus_tag="ON430_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          376 a          163 c          198 g          350 t
ORIGIN      
        1 gtaatccctt cgtatctgaa tatgttaaac gacttgcaaa aggtatttgt caactttgcc
       61 aagaaaaagg gccatttata aaagacggtg taccttattt acattgtcat catatagaat
      121 atttgtcgca aggtggaaaa gatgtgattg aaaactgtat cgcgttatgt cccaattgtc
      181 atgctagaat tcatgaactt gagttacctg acgataaaga aaaattgctg aagatcgttg
      241 aaaaaagaga aaatagcttt tctaaaatac cgtaatttat ttttatagga caaataagct
      301 aatgtgagct atgccttcta tataagacta agttctatta taagtaacga agtaattctc
      361 gttccttcct gtagtaatat tttttaaata acctataggc ttcttcaaaa gtacatcctc
      421 cagtaaattc taagtactgg aggattcttt ttctagtgtt gctttactca tgaaaaagta
      481 gttggctact tcatcaaaat ttctgaactt ttttaatgta taataaagag ctcgatcatt
      541 tactacaagg atgctagctc ctacattttc acagtttttt agcaagtaaa tttttctaaa
      601 atcacatcag gaaatgcatg agattcttta agaaaggcaa ttatagggct atgattacgg
      661 ttcgtgtaag tgacgccaaa aaagaatggc tttcttatat ggctgaattt tatgggatta
      721 ctttatctga acttctaaaa aactattcga tggaacaact agaagatgaa tacaatcgtc
      781 aaacggctga aattgcccat aaacattgga taagaagtgg gaaaacaaca gtattcatgg
      841 aagaatcctt aatgaatttg gtgggctgga atgacctgcc gtttaaaatt cacgcccgaa
      901 attcagaagc agctctgatt ggcaaatacg tgtattggta gagatagaag ataatcaatt
      961 cgttgttacc gctattcaag tagggcaccg aagaaatgtc tatgattaag agactgttta
     1021 tttttacaaa aaaataataa ctggaatcta gttaaaactc ttgaagtgag agatttgact
     1081 agatttt
//
LOCUS       DAJSMI010000312         1077 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJSMI010000312 DAJSMI010000000
VERSION     DAJSMI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.319"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            47..895
                     /locus_tag="ON430_002858"
     CDS             47..895
                     /locus_tag="ON430_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3220710.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          369 a          193 c          190 g          325 t
ORIGIN      
        1 gagaacggaa aaaatgataa tcttattatg aaaatgagga attctcatga gcgtctccat
       61 aagactacaa atcaaaacaa tgtacaacga cttcagccca aaagaacagg ctatcgccga
      121 ttatatttta gaaaatccaa gcaaagtaag tcatagtcct attagcaatt tagcaagtga
      181 actgggcatt gctgactcta ctttttttca atttacaaaa aaacttggtt ataatggatt
      241 taaagatttt aaaatggcca tgttgatcca agaaaatgat ctatctgcta tttctatcca
      301 cgaaaatatt cagaaaacag atactgagct cactatggcg caaaaggtgt tcgagtctag
      361 tatttcaact ttaaatgaca caaaaaagtt gttacaacaa aaggatctaa aaaaagcggc
      421 tactattatc aatgaatcta aacgtgtcta ttttttcggt gttggcggtt cagagattgt
      481 agcaactgac gcttatcata aatttttgcg ttcccctatc gcaacttctc acagcacgga
      541 ttaccatatt caattgatgg aagcttcttt attgacggaa aaagactgtg ctgttttgat
      601 ttctcacaca gggcaatcaa aagaaacaat ccatattgct gaaaccgtaa gaaaaactgg
      661 agcaaaaaca atcgttatta ctagccaagc taattcttcc cttgctaaac ttggcgatgt
      721 cgtttttatt tctatttctg aagaaacaga attccgatct gaagctttag cttcccgtat
      781 ttctcaacta agcattttgg actctctata tgtgattttg atgttttaca acgaaaaaga
      841 atcacgtcgt tctatcgcta aagttcgaca tactatttct gaaatcaaag aatagcacca
      901 taacagaaaa agcgaaaaat ccataaaacc aaaaaagcca ctaccaaata tttggtagtg
      961 gcttttttgg ttctgcaagg gtggtacaga acatcaatag tcggtgggtt agaacgtatg
     1021 tcatcgcatc tttggagaag gttgtattgg tattattcga gttacttgtt agatgcg
//
LOCUS       DAJSMI010000313         1066 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJSMI010000313 DAJSMI010000000
VERSION     DAJSMI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.320"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            26..1045
                     /locus_tag="ON430_002859"
     CDS             26..1045
                     /locus_tag="ON430_002859"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR3220711.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT          359 a          173 c          248 g          286 t
ORIGIN      
        1 caattattta tttggaggaa tatccatgaa acaaaaaatc gcagttcttg gtcctggatc
       61 ttggggcact gctttagcac aaacattggc agaaaacggg catgatgtcc gtatttgggg
      121 aaatgtacct gaacagattg atgaaatcaa taagtatcat acaaatcagc attttttacc
      181 tgatttgacg atcccagaat cgattatcgg atataaagaa ttggcagaag cagtggataa
      241 cgcagatgct attttatttg ttgttccaac aaaagcgatt cgttcagtcg cacaggaact
      301 tattggtaaa atgaatacta agccaatcat tatacatgca agtaaaggac tagaacaaga
      361 tacgcataaa cgaatctcag aagttttggc tgaagaaatt ccagaagaaa aaagacaggc
      421 aattgttgta ttatctggtc ctagccatgc tgaggaagtg gcagtacatg atatcacaac
      481 aattacagct gcaagtattg atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa
      541 tgattatttc aggatttata caaataatga tgtgattggc gtagaaactg gtgcagcact
      601 gaaaaatatc attgcaattg gtgccggcgc tatccatgga ttaggattcg gagatgatgc
      661 aaaagcagca atcatgactc gaggattggc agaaatcagc cgtcttggtg tcgcgatggg
      721 tgccaatcct ctgacattta tcggtttgag tggtgtgggg gacttgatcg ttacttgtac
      781 aagtgttcat tctcgcaatt ggcgtgccgg taacttgtta ggacagggac ataagctgga
      841 agaagttcta gaaaacatgg gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt
      901 agagctagct caacagctag gtgttgagat gccaattact caaacaatct ataatgtttt
      961 gtataatgga gaagatatca aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat
     1021 ggagaatgaa ttttctcttc gttaaataga tgatcaatag ttaatc
//
LOCUS       DAJSMI010000314         1042 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJSMI010000314 DAJSMI010000000
VERSION     DAJSMI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.321"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(68..988)
                     /locus_tag="ON430_002860"
     CDS             complement(68..988)
                     /locus_tag="ON430_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCR3220712.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          324 a          215 c          193 g          310 t
ORIGIN      
        1 gacaaaagtg ttctttactt ttgtcccacg ctctttttag tgagattcgt tgcatcgttt
       61 gtccaaatta ttttccgaca gcaattggac tgttcggaat cgtgaaacca gaagctgtca
      121 attctttgac ataggagctc aagaactctt cttttaaggc atattgtttt ccgttcaatg
      181 cgtagcagac cgtacgaatc gcaaaattgc tgtttccaag atccactaat ccaaagatag
      241 aaggttcagt ttggagctct tcttggtatt tttcagctaa tgtacgattt actcgatcaa
      301 tgatctcata gattttgtca tatccttctt caggaacaat ccgaatatct aaaagtactt
      361 gcatatttgc acgagagaga ttgctgatcg tggtgatgtt tcgattcggg atgaaatgga
      421 cagtaccatc cgttgctttc agctgaaggg tacggatacc aacagatacg acggttcctt
      481 caatcgttaa gtttgctagc ttgatgtagt ccccaacatc catctgttgt tccatgataa
      541 taaaaaatcc ggtaataaca tcactcatga acccttgggc accaagaccg attgccacac
      601 ctgcaattcc ggcaccagcc aaaagtgatc cgatcggcac accgatagca gataaaattg
      661 catagataaa aaagaaacca agggtataat gaaaaatagt atttaaaaga ctatgtaaag
      721 tctttaagcg gctctcgttt agaatggctt ttttagaata ctgattaaaa gacttatcaa
      781 tcagatattt tcccacacgg tgaagaatcc aaaaaaggac gatcaaaaaa atcagatata
      841 aacttttttg gataaaggca gccaaaatgg cgtcccaatc aatcccgtcc caaaaccgct
      901 gaaacgcatt tactttgctg acagcttgat ctgtcaatgt ctcgccagaa gagtctgtcg
      961 taccggcagt tgctaataat gaaaacataa aatcctcttt tctaaaattt gattctttct
     1021 gtattgtacc aagaagtttt ca
//
LOCUS       DAJSMI010000315         1035 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJSMI010000315 DAJSMI010000000
VERSION     DAJSMI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.322"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..>1035)
                     /locus_tag="ON430_002861"
     rRNA            complement(<1..>1035)
                     /locus_tag="ON430_002861"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAJSMI010000316         1029 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJSMI010000316 DAJSMI010000000
VERSION     DAJSMI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.323"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            21..836
                     /gene="thiD"
                     /locus_tag="ON430_002862"
     CDS             21..836
                     /gene="thiD"
                     /locus_tag="ON430_002862"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR3220713.1"
                     /translation="MEDQYVQAMAIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          358 a          181 c          235 g          255 t
ORIGIN      
        1 aaaatggagg aatcgatttg atggaagatc agtatgtaca agcaatggcg atcgcaggat
       61 cagatagcga tggaagcgca gggatgcaag cagatatgca tacattcttt acaagaaatg
      121 tttacggtgt ttctgtcatg accgcatgtg ttgcaggtaa ttcttacgga atcggagcaa
      181 gtgtgacatt gccaacagat tttatcgaca aagaatttga actgattgcg aaggactttc
      241 aaatacgtgc agctaaaaca ggtatgctag cagattcaaa gttgattgag acagttgtaa
      301 aaaattacca aaaatatgat tttggaccat tagtcgtgga tcccgtaatc gtaacgaaac
      361 atggaaatct tttactagaa gaatctgctt tgcaaagctt gaaagaaaag cttgtccctt
      421 tggccgaagt actgacacct aatttttatg aagcagaaaa gctgactgga cggacattag
      481 cagctgaaga agattttgtg caagcagcaa aagaactgca agatatgggt gcaaaaaacg
      541 ttatgatcaa aggacatcat gctaaaaatg cgcagcaaga aacagtcaat gactacgtat
      601 tgttcgaatc aggaaaatca ttttggttaa atgcaccatt ttatcctacc gaccggatca
      661 acggaactgg cgattcgcta tctgcatgta ttgcagctga gttagcgaaa ggaaaaacag
      721 ttgaacaagc gatccgattg gcaaaagact atgtaaaccg tgcaattcgt gaagaaataa
      781 atgtagggca caaatatggc cccatcaatc attgggcagc tggtgaatta gagtaaaaga
      841 aagaagttgt gaaaaagttg ccctgcatca agtaataaga acagccaagt aaaaacagct
      901 tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg aacaccgttt
      961 aagaaagagg tcgtgagcct gccgtccagc tccgagtcac aaggaacaag ttcaaaaaac
     1021 agcttctca
//
LOCUS       DAJSMI010000317         1000 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJSMI010000317 DAJSMI010000000
VERSION     DAJSMI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.324"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>1000
                     /locus_tag="ON430_002863"
     CDS             <1..>1000
                     /locus_tag="ON430_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220714.1"
                     /translation="LSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHN
                     EAENNSLKYWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAP
                     YNYATIQENNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNL
                     INSDPLTTYILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIF
                     VNNNQKYFSFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHT
                     SNQNFFNQINPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGL"
BASE COUNT          423 a          134 c          104 g          339 t
ORIGIN      
        1 atttatctag tataaaaaat tatctaaaaa attcacgacc acttaagtta aatctttgta
       61 tattaaatat ctgtctattc ttaagcatga tccttctgtt aatagcttct acaaaagtaa
      121 taaatatcca taatgaagcc gaaaataatt ctcttaaata ttgggaaaga actactaatt
      181 tatataaaac aaatattact aatcaattaa atagaaacaa caccgtagaa gaaaataact
      241 acttgaaaaa agctagtaaa tttgtttata aaatacagaa gaattataaa acttttatta
      301 tcgcgccata caattacgca actatccaag aaaataataa agaatttttt attggacaaa
      361 aagtttatcc aaattttgaa gactatgtta gtcaacctgc tggtgcagga atatgtgttg
      421 atttaaacta tctaaaatat tacaatccta tttcttttga agaatctact gacttaaatt
      481 taataaactc tgatccacta actacttaca ttctagtacc taaaaaatat aaagaatatg
      541 caaaaatgat tgaaaaaaat tatttagaag acatacagtt tagacttgaa gactctcctc
      601 ctcaagctcc ttataaaact cctaatttga aaaatttaaa aataaaacta atatttgtta
      661 ataataacca aaagtatttt agttttaata ccttatatgg aaaggcaaaa gataactata
      721 caataactga tcccatagtt agagttatta atccagaaat aacagaatca ttattttggg
      781 gaaatatatt gactagtgat ggtggattat tttttgatca gaaacatact tcaaaccaaa
      841 acttttttaa tcaaattaat ccgtatataa aagaatttaa cctagaaaat atcataaatt
      901 tttcttattc tgtttttcaa gaaactggtg aattattatc tcaaaaaaaa gtaaataaat
      961 taaatgcata tctacaatat cttttaatat taggattatt
//
LOCUS       DAJSMI010000318         1000 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJSMI010000318 DAJSMI010000000
VERSION     DAJSMI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.325"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            369..1000
                     /locus_tag="ON430_002864"
                     /pseudo
     CDS             369..1000
                     /locus_tag="ON430_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DAJSMI010000319          987 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJSMI010000319 DAJSMI010000000
VERSION     DAJSMI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.326"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>987
                     /locus_tag="ON430_002865"
     rRNA            <1..>987
                     /locus_tag="ON430_002865"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          307 a          195 c          285 g          200 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagc
//
LOCUS       DAJSMI010000320          972 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJSMI010000320 DAJSMI010000000
VERSION     DAJSMI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.327"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(211..552)
                     /locus_tag="ON430_002866"
     CDS             complement(211..552)
                     /locus_tag="ON430_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR3220715.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(539..868)
                     /locus_tag="ON430_002867"
     CDS             complement(539..868)
                     /locus_tag="ON430_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220716.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
BASE COUNT          290 a          220 c          101 g          361 t
ORIGIN      
        1 atacatcttt aggtgaataa ccacgagcta acagctgtac ataccttcct tttgtcaaag
       61 tatccatatc catcgcctct aaaactggtc gcgccatcta attttcctcc taaataaatt
      121 ccttactcaa tgcctaaggt aatactacct ttttattaat cgtcgtgact ccttctaagc
      181 ctttatctgt aaaatttatt cctccagttt ttattcttct ttatccgcta caaattcaat
      241 gatttcaacg ctgacaatat aatcactcaa atatgaccaa gtatcttcaa cagataattc
      301 gtctccatta tcattcataa gcgtcatttt aaaccattca ttaccttcat catcttcagg
      361 tagcaagccg aatccaattg gattatcagt caatgtttga tcgtataaat catcctcgtc
      421 taatgctaaa acatctaatc ctttacaatt tccgcctctt ctaaacgtca caaatccttt
      481 gtaatcccaa cgttggaaag aaaccttaat cgtatgtgtt ccccatcggc tattcggatc
      541 ataatttttc atttgttgta acctcctcat aatcagttag tactataaag gtatctttgt
      601 ttttatccaa aaaatctaat aaatcttttg tagaagatat ctcttcatca acttcaccat
      661 aataaatacc ttcgataatt gttgctatat ctgaaaaact aatttttact ggacatttat
      721 cgtctaccgc attcaatacc gacaaaattg cttctttttc tgcaatctct cgtttgaatt
      781 tctttggagc taatcgcatg acactcaagt cattttcaag cttttcaatc catgcagttt
      841 tactcatttt cgcaaatttt tgaatcattc ctctaccacc tttgctatct actaaattag
      901 ttttacttca taacgaatat ttttatcgtt tttcgttcac tcaaaaatat ataaaataaa
      961 tcgactcgtt ta
//
LOCUS       DAJSMI010000321          972 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJSMI010000321 DAJSMI010000000
VERSION     DAJSMI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.328"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            7..306
                     /locus_tag="ON430_002868"
     CDS             7..306
                     /locus_tag="ON430_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3220717.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            571..>972
                     /locus_tag="ON430_002869"
     CDS             571..>972
                     /locus_tag="ON430_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220718.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGR"
BASE COUNT          404 a           98 c          198 g          272 t
ORIGIN      
        1 gtttttgtgg aagcaataaa attaatgatt tcagaagaac aggaggaaat gtttctaaag
       61 cagttttatc aaattgctac aaaagctatt gaaaaggctc aagaaaatgc ggggatgaca
      121 agggaatatc tcaatcaaaa agaaatggcg gaatacttaa atgtgagtga aaacttttta
      181 aaaaatgctg tgagaaatga gggattgccc gtagttgaat taggggcaag aaaattttat
      241 tcgaaaaagg cagttaatga gtttatgttt gctcgaatga aaagagggga aaatattgga
      301 agatgatgaa gtagaaatta taaaaaaatg ctttaaaaaa agaaaaaaag tgttcccaaa
      361 agtttgcaga ctgggggaac actcaataat aaagtgctga gaaaacttta tttatagcta
      421 ttatatcaaa aaagaaggtg aaaaggtgtt ttcttctgga aatgttgtag ttgacaaact
      481 tagagaaata aattttgacg gaaatgtaat ccacacacat ggtacgatca attaagaaaa
      541 aaaacgaaaa aaggtgttga gaagccctat ttgaatgcaa tagttatctt agcagaaatt
      601 acctattggt atagacctaa aaaaattttt aacgatgatg gtcagctact tggttatcaa
      661 aaaaagtttc ttgaagatat tttgcaaaaa tcatataagc aattaagcaa aaaaacagga
      721 ttatcagaac gagctataaa agatgcaatt gtgtacttag agaaaaaggg gattatcaaa
      781 agggttttta gaaatattta ttatgagaaa agagcagtta cattatcaaa tgttatgtac
      841 attgttttaa atgtacataa attgatagaa attacttttc ctactttgga ttgcgaaaaa
      901 aaggagtata tgccgaaaat tcgtcagagg ggtatcgaaa aaaagagata tgtctcttta
      961 aaaaaaggta ga
//
LOCUS       DAJSMI010000322          972 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJSMI010000322 DAJSMI010000000
VERSION     DAJSMI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.329"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            120..740
                     /locus_tag="ON430_002870"
     CDS             120..740
                     /locus_tag="ON430_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3220719.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALDFVREQDVFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADTKDPQ
                     RRLVYRSIVQDLENGVAISTISKNYNITRQTVYRIKRDLSSDFPMN"
     gene            765..>972
                     /locus_tag="ON430_002871"
     CDS             765..>972
                     /locus_tag="ON430_002871"
                     /inference="COORDINATES: protein motif:HMM:NF025388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HCR3220720.1"
                     /translation="MKQKQQFTKRQHFIPQFSIRPFEVAKGKCLIIDLKKKPLEVSIK
                     KTADIMQEIDLYESKDSEGNYINRN"
BASE COUNT          363 a          146 c          183 g          280 t
ORIGIN      
        1 tagtttttgt ctatataagg tataccctaa atagacacgg aaatatagtt gaaaaacaag
       61 gtttttcaat gtatgattag ggtgtatttt atatggttgg agaaagcagg taaaaattat
      121 tgaaaattgc atatatccga gtttcatcga ttgatcaaaa tgagcaacgt caaattgagg
      181 aaatgaaaaa atttggtgcc gaaaaaattt tcatcgagaa acaatctgga gcaactatta
      241 ctcatcgacc gatttttcag gaagctctag attttgttag agagcaagat gtttttattg
      301 tagaagccat cgatcgactt gggcggaact atgacgagat tatcgattca gttaattatc
      361 taaagaagaa aaatgtgaga ctcattatca ctagtttacc gatcatggct gaagcaatcg
      421 gaaatcctct tttagataaa ttcatcaaag atttaatcat tcaaatcttg gccatgattg
      481 ctgagcaaga gcgtaccgaa tccaaacgtc gccaagcaca aggtattaag attgctaaag
      541 caaacggcgt ctataaaggt cgccctaagt tatacagtgc agacacaaaa gaccctcaac
      601 gtcgccttgt ttataggagc attgttcagg atcttgagaa tggagttgct atctcaacta
      661 tttctaaaaa ttataatata actagacaaa cagtatatag aattaaaagg gacctcagta
      721 gtgattttcc tatgaattaa agcttagtgg aagggagata aaatatgaaa caaaaacagc
      781 agttcacaaa aagacagcat tttattccgc aattttctat acgccctttt gaagttgcaa
      841 aaggtaaatg tttgataatc gatctaaaga aaaaaccttt agaagtatca ataaaaaaaa
      901 ctgctgatat aatgcaggaa attgatctat atgaatctaa agattcagaa ggaaattata
      961 tcaatcggaa tg
//
LOCUS       DAJSMI010000323          970 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJSMI010000323 DAJSMI010000000
VERSION     DAJSMI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.330"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(33..464)
                     /locus_tag="ON430_002872"
     CDS             complement(33..464)
                     /locus_tag="ON430_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR3220721.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(482..802)
                     /locus_tag="ON430_002873"
     CDS             complement(482..802)
                     /locus_tag="ON430_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3220722.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT          336 a          167 c          133 g          334 t
ORIGIN      
        1 gaaatcggtt tttttaataa ttcttgatgt attcatacat attgccttgc gtaagaatat
       61 tttttcttaa aatagcattg gcagacagca taacaagtgt atctgagctt attctgaata
      121 gaataatact ccataaattt ttcgagatta aactgagatt catcagtcag ttcgttctca
      181 atatagatat ttaataaaat tagaatagct attttatctg cttctgtttc aaattttgag
      241 tgaaaagttg tagaagtatc gtacaaaact gaaaattcaa aatgagaggc gctgaaatgt
      301 gcaagttcat gagataaatg aaaggcttct gcagtttcac cgtatagatt ttcattcaaa
      361 aaaatgattc tgggttttgg atagtagaaa ccaggctctt tcatctccat ataaactact
      421 ttcaaattgt attcgctcaa catttctttc aatttcaaat acatacaaac catcactcca
      481 actattcatt ttcctctaaa gctttagcaa ttgcaatcgc tttacgcatt gtctccttag
      541 atatttcttt tccatcaaaa gaaaaaacag tatcgtcttc tgataaatcc acatgtttag
      601 gggtttctct ttcttctcta cctagaaggt agtctacaga gacatcgaaa tagtcagcaa
      661 tccgacttaa ttcatctgaa ttaggagtat tatttttcca ctttgctaga taaccgttag
      721 aatatccaaa attaatttct aactgccgta tagatatctt tttcctcttc gccaagtctt
      781 ttattatttc ataggtattc atcgataaaa caacctttct aaatgtttac aaaaaaagtt
      841 tagaaaaata cgcagaaata atttgactaa ttctgagtaa tgagctatac tatatcttgt
      901 aaacagattt aacaactaaa aagacaacaa aaaacactat tgatatgtaa atgcagaccg
      961 ccaagaaagc
//
LOCUS       DAJSMI010000324          948 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJSMI010000324 DAJSMI010000000
VERSION     DAJSMI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.331"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            6..887
                     /gene="galU"
                     /locus_tag="ON430_002874"
     CDS             6..887
                     /gene="galU"
                     /locus_tag="ON430_002874"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR3220723.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          344 a          160 c          219 g          225 t
ORIGIN      
        1 agcaagtgaa ggtcaaaaaa gcagtcattc ctgccgctgg gctaggaaca cgttttttgc
       61 cggcaacaaa agccatggca aaagaaatgc ttccaatcgt ggataaacca acgatccagt
      121 ttattgtgga agaagcattg gattcgggaa tcgaagacat cttgatcgta acgggaaaag
      181 aaaaaagacc cattgaagat cactttgatg caaatatcga attggaaaac aatttgcggg
      241 aaaaggaaaa aacagaatta ctcgctttag ttgaagaaac gactcaagtc aatcttcact
      301 tcatccgtca atctcatcca agaggactgg gggatgccgt gcttcaagca aaagcattta
      361 taggaaatga accgtttgtc gtcatgctgg gggatgattt gatgaaagat gatatcccat
      421 tgactcaaca attgatgatg gattatgaag aaacacaagc ttccgctgtt gccgtcatgc
      481 gtgtaccaga aaatgaaaca tctaaatacg gaattattga tcctaaaaca gatagcgaaa
      541 agggtcgttg cagagtaaaa gggtttgtag aaaaacctga aatgggaaaa gcaccaagta
      601 atttagccat tatcggacgt tatctactga ctcctaaaat atttgagata ttagaaactc
      661 aagaaccagg agcaggaaac gagatacagc taacggatgc acttcagact cttaaccaga
      721 cagaggcggt ctatgcgaga gagtttaaag gaaaaagata cgacgtaggc gataagttag
      781 gatatatgaa gacaaatatt gaatatggac ttcaacattc ggaaattggg acttcattaa
      841 gtgcatatat tatccagttg agtaaaaagc taagaacaga aagttaactg gaaataagtg
      901 gcctgctgag aatgaaaggc tatttgttat taaagcggca gcttgtgc
//
LOCUS       DAJSMI010000325          939 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJSMI010000325 DAJSMI010000000
VERSION     DAJSMI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.332"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            79..>939
                     /locus_tag="ON430_002875"
     CDS             79..>939
                     /locus_tag="ON430_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCR3220724.1"
                     /translation="MERRHGEMRLLIPSAKIVPEELHHLGKLPAIIYPINQKIVFDYL
                     YDQYKDVCSAIDIACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSLIGCD
                     EPVIINFADTIINDNIYSLECDSFFYAEDYYSNTWTFFEEKDGDIISVLDKNELKEDD
                     GKKHKLFSGVFQIMDAQYFRECLRKALMSNVVNVNSFYQALQEYSKRYEFLSIKTNNW
                     FDIGHADKYYNSKLEVKAREFNHISIDKDRSILRKISEDVEKFIGEIKWYLKLPAQVE
                     YVRPRIFEYST"
BASE COUNT          345 a          113 c          175 g          306 t
ORIGIN      
        1 ttattgtggc ttttcttaaa aggacgcaaa taatgaaaat ccttaaaaat ataaaaaatt
       61 aacaagcaaa tcttgggaat ggaacggagg cacggtgaaa tgagattatt gattccatct
      121 gctaagattg taccggaaga acttcaccat cttggaaaac ttcctgcaat tatctatccg
      181 attaatcaaa aaattgtatt tgattattta tatgatcaat ataaagatgt atgttctgct
      241 atcgatattg cctgttacga aaagatggat aaggtagcaa gacgattaga taagtatatt
      301 aaatctaaaa cagtaaatat tattcagctt aaagagttag gggatcttgg aagaactata
      361 tatgattctc taataggatg tgacgagcct gttattatta attttgctga taccattatc
      421 aatgataata tatattcctt ggaatgtgac agctttttct atgctgaaga ctattatagt
      481 aatacttgga cattctttga agaaaaagac ggagatatta tttctgtttt agataagaac
      541 gagttaaaag aagatgatgg aaagaagcac aaactgtttt ctggtgtatt tcaaattatg
      601 gatgcccagt attttagaga gtgtttgcgt aaagctttga tgtcaaatgt agtaaatgtg
      661 aatagttttt atcaagcttt acaagagtat agcaaacgat atgaatttct ctctattaaa
      721 acaaataatt ggtttgacat tggacatgca gataaatatt ataattccaa attggaagtt
      781 aaggcaagag aatttaacca catttcaatt gataaagatc gtagcatcct gagaaaaatt
      841 agtgaagatg ttgaaaaatt tatcggagaa attaagtggt atcttaagtt gccagcacag
      901 gtagaatatg tgagacctag aatattcgaa tattctact
//
LOCUS       DAJSMI010000326          930 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJSMI010000326 DAJSMI010000000
VERSION     DAJSMI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.333"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            13..>930
                     /locus_tag="ON430_002876"
     CDS             13..>930
                     /locus_tag="ON430_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3220725.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTTFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRDRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNL"
BASE COUNT          326 a          148 c          182 g          274 t
ORIGIN      
        1 gaggactttc tcatgactaa atttactata gaaattatgc aaacactact caataaaggg
       61 gatttagatg aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca
      121 gagctaacta cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat
      181 tcccgtaacg ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact
      241 attcctagag atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc
      301 aatgattcat tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa
      361 atctccaaat taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac
      421 atgagtaaat tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat
      481 tattccgtta tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag
      541 gaagcagtgt atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt
      601 actattgccc caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa
      661 cgtgggttag aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt
      721 atccatagtg tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat
      781 atcgctcata aagttcgtgt tcgagatcga aaagaaattt gtgaggattt caaattggtt
      841 taccaagcga attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg
      901 aaaaagcagt atccaagagt cgtcaattta
//
LOCUS       DAJSMI010000327          930 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJSMI010000327 DAJSMI010000000
VERSION     DAJSMI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.334"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(5..913)
                     /locus_tag="ON430_002877"
     CDS             complement(5..913)
                     /locus_tag="ON430_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3220726.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT          312 a          109 c          164 g          345 t
ORIGIN      
        1 ttttctattt taaggataga taatactttg aaggggataa ccctgtatat tttttaaaag
       61 ctgaagaaaa atgatggatc gaatgaaatc ctaatatatc tgcaatttct gtataggtta
      121 attcattttc tagcacgaga cgttttgctt tttccatttt tagttcttta aaatagctaa
      181 taacactttt tccattcact tttctaaaat gtttttttaa gctggctgga ctgataaaaa
      241 aaatttgggc taattgttca agcaaaaaat tttctgaaag atgttcttct aaatatcgta
      301 tgattggatc aactaattca tctgaataaa gaatatgctt ttttttagga atgactttct
      361 catttgaagg tattgtcaat aaagcaagtt cctgttgata atgccttggt aaggaagaca
      421 attgaatttt ttgcagacaa taagtaacta ataaggaaag caaatgaatg ctcatctgtt
      481 ggcatagtat gaaaaaagaa ttgatttcct gatcatagga aatagctggc ttactatgtt
      541 ttagataatc aattttgaac tgttcatttt tcagaaaagt aatgatagat gttatttctt
      601 gatctataga aaaagattgt cccgtaatat gtgctaataa ttgctcatca aaataaaact
      661 ggataatgat aaaagtttgt gtttcctttt gagtagaaaa gatttggact tcttgctgtt
      721 tatcaatgaa taaaagggaa ggattatcta gttctataga tttatgtgca aattcagcat
      781 ataaattagc gctttcattt attagtagtc gaaaataggg agcagcagat aaatgaagat
      841 gggtatctat ggaataggtt agtgtttttt tgacaagatt ggaggattgg ttgtttgtta
      901 acaaaagaac cactttcctt tctcttttta
//
LOCUS       DAJSMI010000328          910 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJSMI010000328 DAJSMI010000000
VERSION     DAJSMI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.335"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..888
                     /locus_tag="ON430_002878"
     CDS             <1..888
                     /locus_tag="ON430_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCR3220727.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          389 a          120 c          149 g          252 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc
//
LOCUS       DAJSMI010000329          908 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJSMI010000329 DAJSMI010000000
VERSION     DAJSMI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.336"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..>908)
                     /locus_tag="ON430_002879"
     CDS             complement(<1..>908)
                     /locus_tag="ON430_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3220728.1"
                     /translation="VDENRAKQRLRIYYAKTSVIGNTIEVKAEPIFNDIRKSVLNKYD
                     SGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKANFLEFFGGKEGSIL
                     DRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQSVLVGVYPFISSSS
                     EGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAAKDWLKTNIDKQKP
                     QVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSARIVEYTFNVLTNS
                     YDKLVVGNVKTNFLENTENNVSNLIN"
BASE COUNT          259 a          187 c          132 g          330 t
ORIGIN      
        1 tcattaatca aattgctgac attattctct gtgttttcta agaagtttgt tttgacgttt
       61 cctacaacta atttatcgta tgaattggtt agaacattaa acgtatattc cacaattctc
      121 gctgacatat tcacttttaa ctgtggatga tacacatcta ctccgtcacc catcgaaact
      181 ttttctagat caacaaattt ttcatagcct ctttgatgcc tcaatggtac taattcaatc
      241 gaaccactca cttgtggttt ttgtttatct atgtttgttt tcaaccagtc tttagcagct
      301 tcccttaatg tggctacatc agtcgctttg tctttaaaat caacaaaaga aacatatcca
      361 gcaggataat catccacgta atccgtgaaa ataacttctt ctggtagagt gatctcgtct
      421 tctccttctg aagagctgct aatgaatgga taaactccaa ctaaaacact ttgagcatca
      481 atctctaagt caagaccagt taagttttta gtataaatcg ctttgatttt atgatccgta
      541 cctagacttt tttcatgacg taatgtgtta ttatctttta gaaactcacc atgaaatcga
      601 tctagaatag atccctcttt tccaccaaag aattctaaaa aattcgcctt ttctatcttc
      661 acattagcaa gcgtatctac taatgacgag aaagaaaact gcgaagggat agctggtttc
      721 gctaaaactt ttgcgttttg ccatgcctga gtagcagtga tcttttctgt tccgctgtca
      781 tatttattca acaccgattt tcttatatca ttgaaaatag gttcagcttt tacttctatc
      841 gtattgccta ttacagaagt ttttgcataa taaatccgta gacgctgttt tgctcgattt
      901 tcatctac
//
LOCUS       DAJSMI010000330          891 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJSMI010000330 DAJSMI010000000
VERSION     DAJSMI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.337"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..774
                     /locus_tag="ON430_002880"
     CDS             <1..774
                     /locus_tag="ON430_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3220729.1"
                     /translation="HFLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAE
                     LPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIR
                     ALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAK
                     YAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEV
                     PKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          318 a          173 c          157 g          243 t
ORIGIN      
        1 cattttcttg aaaatctaac gtacaaatgt aatacgcttg ctcgagaaat gcgagacgaa
       61 tcgacgagtc tttttagtgc aaccctcatt tcactcatga cggaagattt cacattggat
      121 gaacttgctg agcttccttt agaagctttc tgtgatttac ttcaagaaaa aggaaaaggc
      181 cgtttcaagc aaccagaaaa aatcgctaaa gcaattcaac gtgcaattac catgagttac
      241 cgcctaggtg ctcttgcaca agaatcaatt aatgttgttt taagtgttct cgtacgagaa
      301 atacgagcac ttgaaaaaaa tatcaaagaa ttagataaag ccatcgaaca agtaattgtt
      361 gtcattccag aatatcaatg cttaaccagt attccaggcg ttggtaaagt ctatgctgcc
      421 ggtttaatcg ctgaaattgg tcaaatcgaa agatttgaag atcagacaaa attggcaaaa
      481 tacgctggat taagttggaa agtgaaccaa tctggaaact atcagtcgca aaatacacct
      541 ctgacaaaac aaggaaatcg atattttaga tactacttag ttgaagctgc caactctgta
      601 aaaaattatc ttcctgaata caaagctttt tatcaaagta aatataagga ggttccaaag
      661 catcaacaca aacgtgcact cgtcctaacc gcaagaaaat ttacgcgtct ggtggatacg
      721 ctactacgta aacaccaact ctatacgcca ccaaggagtg tgatagagaa ataacaatcc
      781 gttattgacg caaatccttg aatcaacccg aaaaaaattt gattttgatc gggtctagtt
      841 tcgtgcgctt caaaacatat tactcaataa agaaaatctt aaatttcatc t
//
LOCUS       DAJSMI010000331          833 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJSMI010000331 DAJSMI010000000
VERSION     DAJSMI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.338"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(260..595)
                     /locus_tag="ON430_002881"
                     /pseudo
     CDS             complement(260..595)
                     /locus_tag="ON430_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(607..>833)
                     /locus_tag="ON430_002882"
     CDS             complement(607..>833)
                     /locus_tag="ON430_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220730.1"
                     /translation="NERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLN
                     RTINAHEEKYHASKAYEEQQRRIQMEQERQ"
BASE COUNT          211 a          173 c          127 g          322 t
ORIGIN      
        1 gcttttgcag ctttgtctgt gactacaaaa gctctgcctg tttgaatttc tgtcacaaaa
       61 acgagattgt ctctgctttt ttcaacaaca actccaatag ttgaataagc catttctgct
      121 tcctcccttc tctttctttt gatataatgt agtcggaaat cgcaccactc aatgaattgg
      181 cggaaaacaa ttcagtgaaa aaaaagagtc ctggacaatc acgttcagga ctcttttact
      241 tcttttattc acttaataac taacactctg tgagtgaaat agactctttt atgcttttac
      301 atggaaagtt attgattccc tcttcattca tttcccaagt agtataataa ggtgttaact
      361 tggcctcata ttcttgaatg ataaaatatt tttcaccgtt cacatgatta caacaataca
      421 caccagtagc tgggtactca acgactaaaa attaatttgg atcaatctgt tcgcttctca
      481 acatttctct tatctgaaac aaatgctctt tttccaaaca atcgggaagt tgttcgattc
      541 gttctttttt tagtgatcct tctatgactt tccattgatt caaatatcgt ttcatttctc
      601 ttcctcctac tgtctttctt gttccatttg aattcttcgt tgttgctctt cgtatgcttt
      661 acttgcgtgg tatttttctt catgggcatt aatcgttcga tttaattgac gcaaactccg
      721 ctgcgtgaca actctttgtt ggaattgttt cctgcgcttc atgaattctt cgtaactata
      781 tttgtattca ttcgttggca attgtttaag cgttcgaaag gatcgttcat tgt
//
LOCUS       DAJSMI010000332          804 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJSMI010000332 DAJSMI010000000
VERSION     DAJSMI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.340"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            69..770
                     /locus_tag="ON430_002883"
     CDS             69..770
                     /locus_tag="ON430_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3220731.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          291 a          147 c          138 g          228 t
ORIGIN      
        1 taaaacttta caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg
       61 actttttaat gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg
      121 attttgaaca tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt
      181 cttataaaaa aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct
      241 tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg
      301 actatgtgaa tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca
      361 cttatacagc tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg
      421 aaaaattagc acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag
      481 tagttgaaat acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg
      541 taaaacaaaa tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata
      601 tcaccattcc ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg
      661 aaactgctgg acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa
      721 agcgttttac tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa
      781 aataagcagt gaccctgata aata
//
LOCUS       DAJSMI010000333          801 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJSMI010000333 DAJSMI010000000
VERSION     DAJSMI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.341"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            7..600
                     /locus_tag="ON430_002884"
     CDS             7..600
                     /locus_tag="ON430_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010828615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3220732.1"
                     /translation="MSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLLNNLSLIDK
                     QYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKFNSNISND
                     HIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGNLDKENS
                     TFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
BASE COUNT          316 a          119 c          112 g          254 t
ORIGIN      
        1 ttcaccctgt caattaataa aaaaaagatc aaacaaaatt ggaacttttc aatatcagaa
       61 aaagatttta ttatagtaac aggaaaaagt ggtagtggaa aaactactct gttaaataat
      121 ttaagcctaa tagataaaca atataccggt aacttattct attttgaaaa gaaagtttct
      181 aaacatgata tacaacgtct acgaagaaat catattagct atttatttca aaactatgct
      241 cttcttgaaa atcaaacagt acaatacaat ttcgaattag caaaaaagtt taacagtaat
      301 atttccaatg atcatattta taatttatta acagattttt ctttgccaaa agatatctta
      361 aaagaaaaaa tctttctctt aagcggtgga gaacaacaaa gaatagcatt aattagaaat
      421 atgttaaaac catttgatat attatttgca gatgaaccta caggaaattt agataaagaa
      481 aactcaactt ttataatgaa ctttctgaaa aatttaacag aaaaagaaaa aaaagccgtt
      541 gtactagtaa cacatgactt aaatgttcta aactatggaa ctaatattat agaattataa
      601 tttttacccg tgatgctagc tgatttaaaa ctagcaccac gggtatttat tttatattcc
      661 atctctccta tcccaaaatt taacagaatt aggcagaagt cgcccatcct ctataggtgg
      721 gtgatgaatg ccgttcggta tgagggttac cgttggtaac tcgaagaccg acaactaagg
      781 cgtattgcct ttttattttg t
//
LOCUS       DAJSMI010000334          790 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJSMI010000334 DAJSMI010000000
VERSION     DAJSMI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.342"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
BASE COUNT          304 a          101 c           98 g          287 t
ORIGIN      
        1 gcgttttata actttacgca agatcataat tttgtctgtg tagatatttc gttatatcac
       61 taaagataat aagatttaat gacctcatta atattgtttg ctaggtccaa actcttatta
      121 aactttcctt gctataaaac ccaaacatag taataaaaga ataacttatt acctttaaat
      181 tatactacca ataaatggta gtatattatt aatttatata atatgaaacc taaaatatag
      241 tagtatgtta atttatacta aattaaataa agtactacct ttaataggta gtactttatt
      301 atcaagccat cattccttct tctactagtt ctttttgtca cataattttg caatcaataa
      361 atgttgcgca ataagtataa gaattccatt ttctaaattt gaaaggtgat ccataatttc
      421 atgttcaagt agtttattaa ttgtccactt gtcgcttcat ctaaaaattt tataggtttt
      481 aggaatgcta ttcgcttatt ttaggaagat tttaactgtt tgctatagat atatatcata
      541 ctagtatcgt aataaaagta ctctaaaaga agaattttca aatacgcaaa attaagtacg
      601 aaagagttga aacaaatttg gagaaaaatg agcatagaat gtctgatgag ttaaatagac
      661 ctaaaaatta tctaaaagtg gagcaaaatt aggaattaca aacaaaacac taaattagtt
      721 tatataattt tccgtaatat ttaatatata tgaaaaggaa aacgtagaaa attacgtcta
      781 cgttttccta
//
LOCUS       DAJSMI010000335          789 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJSMI010000335 DAJSMI010000000
VERSION     DAJSMI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.343"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(120..518)
                     /locus_tag="ON430_002885"
     CDS             complement(120..518)
                     /locus_tag="ON430_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR3220733.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(515..751)
                     /locus_tag="ON430_002886"
     CDS             complement(515..751)
                     /locus_tag="ON430_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3220734.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          246 a          138 c          118 g          287 t
ORIGIN      
        1 attaatccat attttccata tttttatgaa aaaactattt tgtatgatta aacataaaaa
       61 aatcagcata ttccttaatg acatgctgat ctaattgaga aataaagttt ttaattcgtt
      121 cattttattg cggtttgtcg taaataactg gttatcttgg actttagtga atcgaaatcc
      181 aatgaactag tggtaatcat tatgataaaa tcaacagctt cttgacgaga aaaagcaact
      241 ggacaattat tcaattgaag aaacaacaac atggcaacaa gagcagttcg tttattgcca
      301 ttatgaaaag gatgtttttt agctaaatta attgctaaaa tcgccgcttt ttcatagatt
      361 tcaggataga gttcttgatt gaatactgct tgtgaaggct gtttgacggc catatctaat
      421 gcatttgcat cttttacacc gatcatttcg cctggcgagt gacgtaaaat aactttcgcg
      481 ttgattttga taatttcttt agtcgaaaga tacctcataa atcaaccaaa tcttttaacg
      541 cgtcttcata ttgattcatg atcgaatcaa ctaatgaatc aatatcaggt tcttcttgtg
      601 ctttgatcat tcgtacagaa ccatcagatt gaaaaacata actgattgcg tcacctgtct
      661 gcacacccat gaattctttt acttctggtg ggatagttgt tacatcgcta ttgccagatt
      721 ttctaatttt atactttttc atttttttca tatttcgcca ccctctatac cattctcatt
      781 ctatagtaa
//
LOCUS       DAJSMI010000336          784 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJSMI010000336 DAJSMI010000000
VERSION     DAJSMI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.344"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(191..544)
                     /gene="tnpB"
                     /locus_tag="ON430_002887"
     CDS             complement(191..544)
                     /gene="tnpB"
                     /locus_tag="ON430_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR3220735.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(534..728)
                     /locus_tag="ON430_002888"
     CDS             complement(534..728)
                     /locus_tag="ON430_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220736.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          227 a          159 c          138 g          260 t
ORIGIN      
        1 gttgtaagat tctgaatggt ttgttgttgt gtttgattgg ttttatgggc ttcttgaagc
       61 aactgaagca acgtatctgt agtttccaaa atttttcacc accttgaatc gaactgattt
      121 tgttacagaa taccacaaga aacgcacaag aaataggggt tcaacacatt ttttgtgtta
      181 accccccagc ttaaaaaact cctttttttc ctggcaaaat agctcgaggt tgatcgatac
      241 ttaatccttc taacaaccat cgaacctgtt ggttcgttaa ggttcgaact tcgtctttcg
      301 tccgaggcca ttttaacttg ccgttttcta tccgcttata acagagaaga aaaccatcac
      361 cgtcccaata gagaagtttg aaacggtctt gacggttccc gcaaaataaa aaaacggcat
      421 cttcgtaaag ctcgagttga tactcttgtt gaataagact ggcaagacca tcaattcctt
      481 ttcgcaaatc tgtttttccg cacacaatat aaatatgttt ggtttgaagt aaatcataaa
      541 gcattggaca acagttcttt caaaatagct tgaagaatat aattgttaat accgttatag
      601 atcaaaactt cagaaccatt ctctcttttg atgcgcatcg ctaacttggc agccgcgggt
      661 ttaccttttt ctggtgcact cttttcgttt cctaattgga caggaacaat ctcatttaat
      721 ttatacatag aaaacctcca gacttcttga tagctcaagt atatcttgag gtgcatttgt
      781 tagt
//
LOCUS       DAJSMI010000337          783 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJSMI010000337 DAJSMI010000000
VERSION     DAJSMI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.345"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(254..589)
                     /locus_tag="ON430_002889"
     CDS             complement(254..589)
                     /locus_tag="ON430_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220737.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            complement(619..774)
                     /locus_tag="ON430_002890"
     CDS             complement(619..774)
                     /locus_tag="ON430_002890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220738.1"
                     /translation="MKRRKQFQQRVVTQRSLRQLNRTINAHEEKYHASKAYEEQQRRI
                     QMEQERQ"
BASE COUNT          200 a          172 c          116 g          295 t
ORIGIN      
        1 gcttttgccg ctttgtctgt gacgacaaaa gctctgcctg tttgaatttc tgtcacaaaa
       61 acaagattgt ctctgctttt ttcaacaaca actccaatcg ttgaataagc catttcttct
      121 tcctcctttc ttttgatata atgtagacgg aaatcgcacc actcaatgaa ttggcggaaa
      181 acaattcagt gaaaaaaaag agtcctggac aatcacgttc aggactcttt tacttctttt
      241 attcacttaa caactaacat tctgtgagtg aaatagactc ttcaatgctt ttacacggga
      301 agttattgat cccctcttca ttcatttccc aagtagtata ataaggcgct aacttgccct
      361 catattcttg aatgataaaa tatttttcac ccttcacatg attacaacag tacacaccag
      421 tagctgggta ctcaacgact aaaaattgat ttggatcaaa ctgttcgttt atcaacattt
      481 ctcttatctg aaacaaatgc tctttttcta aacaatcggg aagttgttcg attcgttctt
      541 ctcttaatga tccttctata actttccatt gatctaaata ttgtttcatt gctattcctc
      601 ctactgtctt tactgtcttt actgtctttc ttgttccatt tggattcttc gttgttgctc
      661 ttcgtatgct ttacttgcgt ggtatttttc ttcatgggca ttaatcgttc gatttaattg
      721 acgcaaactc cgctgcgtga caactctttg ttggaattgt ttcctgcgct tcatgaattc
      781 ttc
//
LOCUS       DAJSMI010000338          779 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJSMI010000338 DAJSMI010000000
VERSION     DAJSMI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.346"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(57..377)
                     /locus_tag="ON430_002891"
     CDS             complement(57..377)
                     /locus_tag="ON430_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR3220739.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT          291 a          146 c          112 g          230 t
ORIGIN      
        1 aattgaatat taaataaaaa ggcttatttt ttctgatcag tacttattcc aagtgtctag
       61 tggccgctag taatcttgat gccaatgata ctgatgagta gcatccccac aaagaaccaa
      121 gtggaaagat tcagcttatc atgaaacaag accacaccaa ttatgaccga accaactgcg
      181 ccaatccctg tccagatagg ataagagaga ctcatcggca aacttttgat tgcctttgac
      241 aaaaagtaaa aactagcaat catgccaatc actgtgtaaa aagaataatt gattttagaa
      301 aaaccatcac tccatttcat catcgttgac cagaatactt ccaaaattcc tgctatgatt
      361 aattccatcc atgccatact tacattcctc cttaactata aaaaaataca caaaaagagg
      421 aaaatcatcc cttcaaagac ctaatggaat gatcatcctc tttatcctac ccggtggcag
      481 attgtatatc tttttctgta gtatagcaca cctatccaga tatcggcaat ctcatacttt
      541 ttagtaaagt gtaccaagca ctgacggata tatcgttagc ggaaaagaag atgaatgctt
      601 tgcaatttca tagaaagaag ttgttattta ttaatcattt aaaaaaaaca gtaactagac
      661 cttatttcta tatattatcg ggcaataaat taaaaaaagc tttaccttac aataagtaag
      721 ataatttttg tgataaaaaa ataacgaaaa atatttaaaa atagtgtcta tttcgtgag
//
LOCUS       DAJSMI010000339          773 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJSMI010000339 DAJSMI010000000
VERSION     DAJSMI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.347"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>773
                     /locus_tag="ON430_002892"
     CDS             <1..>773
                     /locus_tag="ON430_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3220740.1"
                     /translation="NNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAKQNNLKL
                     GDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEI
                     LNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSG
                     IKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIF
                     ISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLIN"
BASE COUNT          312 a           67 c          130 g          264 t
ORIGIN      
        1 caaataatac taaaagaaat attttattta gtagtggagt atttacaatt aaagaaggta
       61 aaaatataga agaaaatgat aagaattcaa ttattgttca tgaagaattt gctaaacaaa
      121 acaatctaaa attaggtgat gaagttgatc ttgaattact agatattgaa aaaagtggaa
      181 aaataaaaag tcataaattt aaaattatag ggatcttttc tggtaaaaaa caggaaacat
      241 atacaggatt atcttctgat tttagtgaaa atatggtgtt tgtagattat tcaacaagcc
      301 aagagatatt aaacaattca gaaaataatg aaattgcaaa taaaatttta atgtattctg
      361 gtagtgcaga atctacagac ttagccttaa acaaattgaa agagcttaaa attgatgagt
      421 caaagtattt tgttgaaaaa gattctaatg cgtttgaaga gtctttagag tcagtgagtg
      481 gaataaaata tataataaaa gtaatgactt attctattat gttgggtggg atggttgttc
      541 tttcattaat cttgattcta tggttaaggg aaagaattta tgaaataggt atattcttat
      601 ctattggaac atctaagata caaattataa tgcaatttat attcgagtta atattcatat
      661 cgataccaag tataatatct tccttatttt taggaaatgt attactaaaa gtaattgtag
      721 atggattcat taactcagag gattcaatga tttccggtgg aagtttaata aat
//
LOCUS       DAJSMI010000340          752 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJSMI010000340 DAJSMI010000000
VERSION     DAJSMI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.349"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>752
                     /locus_tag="ON430_002893"
     CDS             <1..>752
                     /locus_tag="ON430_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR3220741.1"
                     /translation="SFVISYIQSYFTINFNHFKYLILSFISTFLNIAISVALMQTLYF
                     DDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIV
                     LAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKV
                     KKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPV
                     NAEFYEKKTSFIAIGTVASAIVNILLNMIFIP"
BASE COUNT          233 a           94 c          131 g          294 t
ORIGIN      
        1 tagttttgta attagttata tccagtctta ttttacgatt aactttaacc attttaaata
       61 tttgatttta tcttttatat ctactttttt gaatattgca atctctgtag ctttgatgca
      121 gacattatat tttgatgata aatatgttgg cagagtagtc ggcggaacac tgtcatttgt
      181 gttgataggt ttagtaattt atttaagaat aatgataaag ggtgctcatt tttttgataa
      241 aaaacagtgg agatacgctt taaaaatctc tttgccgcta atcccacatt ccttagcaaa
      301 tatagtttta gcacaattcg atagaattat gattaattct tatttaggtc ctgctgcggg
      361 gggaatatat tcctacatta gtaacatagg tattattcta agcgtaatat ggatgtctac
      421 taataacgcc tgggttccat ggttctatgg tgagatgagt aaaaataatg aaaaaaaagt
      481 gaagaaagtt agtaactatt atttagtagt ctttactttg attacgatga ttgtaatggt
      541 agtttcgatt gatttagcaa aatttatggc tccgaaagac tattattctg ggttatcttt
      601 agtggttccg ataaccttag gctatttctt tcagttttta tatagtttac cagttaatgc
      661 tgaattttat gagaaaaaaa catcgtttat tgcgattggt acagtagcaa gtgctatagt
      721 taatatacta ttaaacatga ttttcatacc ta
//
LOCUS       DAJSMI010000341          748 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJSMI010000341 DAJSMI010000000
VERSION     DAJSMI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.350"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(233..382)
                     /locus_tag="ON430_002894"
     CDS             complement(233..382)
                     /locus_tag="ON430_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220742.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          262 a          118 c          109 g          259 t
ORIGIN      
        1 attatacaga taataacgga atattatcat cgaaacagct aacaaaattg tcataaaaaa
       61 cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa gagagacaat
      121 ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt tacaaacgtc
      181 ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact ccttatctag
      241 attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg ttattttcgt
      301 tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg cggtattgca
      361 tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt agttcttgaa
      421 agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt ttgatgcaat
      481 taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc tattttcttt
      541 taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa tataaatcat
      601 atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg aacaacatag
      661 gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat agttatctaa
      721 aaagcagaaa gatactcaaa ataaaaaa
//
LOCUS       DAJSMI010000342          747 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJSMI010000342 DAJSMI010000000
VERSION     DAJSMI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.351"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            330..>747
                     /locus_tag="ON430_002895"
     CDS             330..>747
                     /locus_tag="ON430_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCR3220743.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELE"
BASE COUNT          282 a           83 c          124 g          258 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagt
//
LOCUS       DAJSMI010000343          723 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJSMI010000343 DAJSMI010000000
VERSION     DAJSMI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.352"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            129..>723
                     /locus_tag="ON430_002896"
     CDS             129..>723
                     /locus_tag="ON430_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3220744.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQ"
BASE COUNT          305 a          105 c          103 g          210 t
ORIGIN      
        1 taaaaaaatg tatttatttg tatttataag aataaagtat catactccag ataaaaagta
       61 aatactttta aatactttta ttttataaat atgatatact atctttaaaa ataaaaagga
      121 gaaaaaagat gaagccaaat ccttcagaag caggaaaacg aatcaaacaa ttacgccttt
      181 catgtggttt tactatggaa gaacttggaa ggaaaataga taattcacca cgtgcgacca
      241 tatccaactg ggaacgtggg accaatttac caaatccaca aaagctgaaa ttattatcaa
      301 ctatcactaa ttcaacaatt gactggatta aatggggaac tttagaagaa tatataactt
      361 cataccttat cgatataggg tatgagctat atattaagga cttcccagaa atacctcaca
      421 aagtctttaa agacattcaa gaaagatata gtgatacgtt ttcgctagat aaagactatg
      481 agttattgaa tcctataata aaaaatatat ttacaaaata ttactcaaaa gattttgaaa
      541 tttatctaaa tgagatactt gctaataata tcaatcctct gattgaagag tacgctaacc
      601 aaagacactc tatctctaaa gagctatttt tcaaaagagc caacagtaaa cttcaagaca
      661 agataagaaa tggtgatttc aaatacggtg aaaaagaaag aatgattgat tctttaaaac
      721 agc
//
LOCUS       DAJSMI010000344          721 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJSMI010000344 DAJSMI010000000
VERSION     DAJSMI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.353"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..>721)
                     /locus_tag="ON430_002897"
     CDS             complement(<1..>721)
                     /locus_tag="ON430_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR3220745.1"
                     /translation="IFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKI
                     KWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQH
                     SSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELK
                     RAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFIL
                     SIFIDEMVEAHVLFNKRDVISI"
BASE COUNT          274 a          126 c           89 g          232 t
ORIGIN      
        1 aattgaaatg acatcgcgct tattgaaaag tacatgagcc tccaccattt catctataaa
       61 aatagatagt ataaaaatta aaacaatacc aagaaacgca taattcactt tacttctaaa
      121 aaatgttttg agtaaactaa agatatttaa cacatagaaa gcatagatac atataaaagc
      181 aattatacca cttgatacaa gtacttgaag aaatccatta tgcatgtttc catgtgctct
      241 ttttagttca ttcacatctg tagtatcaag atctactttt ttcaaaaatt ttgcatcgct
      301 attcccatag aaatcggtct ctccataacc aaataacaaa gttggtctta taactttaag
      361 cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg gagttatcac tagaatgctg
      421 taatattact tctttttctg gaagtggagt tttatctgat tcatttgact ctataacttc
      481 tacttttcta tcaatcgata caggaattgt tgctaaaaaa tccttagcat aattagtacc
      541 aaaagaaaaa tataccaata gaactaataa tatgcttacc atccatttga tttttacagg
      601 tagtacttct ctaatgctct ttctaaattt tgaattggca aacaacaaaa gaaataatac
      661 taaaaaagaa caaattacta cctgggcacc tctagacgaa gaaagtgtaa tataaaatat
      721 t
//
LOCUS       DAJSMI010000345          696 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJSMI010000345 DAJSMI010000000
VERSION     DAJSMI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.354"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
BASE COUNT          240 a           94 c           94 g          268 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttct
//
LOCUS       DAJSMI010000346          696 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJSMI010000346 DAJSMI010000000
VERSION     DAJSMI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.355"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            57..>696
                     /locus_tag="ON430_002898"
     CDS             57..>696
                     /locus_tag="ON430_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR3220746.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSA"
BASE COUNT          261 a           88 c          138 g          209 t
ORIGIN      
        1 ttttaaagat taagaatcga attatagatg actataattc ataggaggag atataagtgt
       61 ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt gaaattgttc
      121 gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt gaagaaaaac
      181 aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta attggtgata
      241 tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt tttcatgctg
      301 ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta aaaacaaatg
      361 tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa cattttgtag
      421 gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca aagttattga
      481 gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca atattttctt
      541 gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt tttagtaaac
      601 aaataaaaaa caataaagat attactttga cagatgttga tatgacacga tttatgatgt
      661 caataccgga tgctgcaaga ttagttctgt ctgctg
//
LOCUS       DAJSMI010000347          695 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJSMI010000347 DAJSMI010000000
VERSION     DAJSMI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.356"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>695
                     /locus_tag="ON430_002899"
     CDS             <1..>695
                     /locus_tag="ON430_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HCR3220747.1"
                     /translation="GSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVIAQNGA
                     FDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVETEKKSAASPRLFSLSSLQS
                     KMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAE
                     AIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAM
                     FAEKYTYEETTIL"
BASE COUNT          248 a          138 c          144 g          165 t
ORIGIN      
        1 caggaagttt ttccttaggc agagtccaaa ccccaacgct ttacatgatt tatcaattac
       61 aggaaaagat tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg
      121 ctcaaaatgg cgcatttgat gcgaagcttg atccgaatga aacccaagcg acgcaagaag
      181 catttgaaga ttatttaaaa gaaaaggggg ttcaattggg aaaacagcca ggtacgatcc
      241 accaagttga aaccgagaaa aaaagtgcgg caagtccacg tcttttttct ctatccagcc
      301 ttcaatctaa aatgaatcag ctcatgaaag ctagtgccaa agatactttg gaagccatgc
      361 aagggttgta tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg
      421 aaggggaata tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg
      481 aagcgattcc gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc
      541 aggaacatta cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac
      601 taagtccact gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg
      661 cggaaaagta tacctatgaa gaaacaacga tcttg
//
LOCUS       DAJSMI010000348          678 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJSMI010000348 DAJSMI010000000
VERSION     DAJSMI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.357"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..660
                     /locus_tag="ON430_002900"
     CDS             <1..660
                     /locus_tag="ON430_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HCR3220748.1"
                     /translation="VSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSR
                     GVQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKA
                     VYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLI
                     ESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFT
                     E"
BASE COUNT          248 a          123 c          117 g          190 t
ORIGIN      
        1 gtatccaaag aagcgattta tattgccatt ggtatacgag aagacggcac taaagaagta
       61 ctgagttatg cgattgctcc aactgaatca acatacgttt ggaatgagct gctacaggat
      121 attaactcca gaggagttca agaagtcttg ctttttatta cggacggctt aaaaggcatg
      181 aaagatacta tccatcaaat ttatcctaaa gcaaaatatc agcattgttg tatccatgta
      241 tctcgtaaca tcgctcataa agtacgtgtc aaagaccgaa aagaaatctg tgatgacttt
      301 aaggctgttt atcaagctaa ctcaaaagaa gaagcgaata ccttcttatc cggcatgatt
      361 gagaaatgga agaaaaacta tcctaaagtg acgcagtcac tcatagaaaa ccaagactta
      421 ttaacttttt atgattttcc acctagcatt cgtagaacca tttactcaac caatctaatc
      481 gagtctttca ataagcaaat taaaagatac agccgtagaa aagagcagtt tcaaaatgaa
      541 gaatcactag aacgctttct agtcagcatt tttgatacat acaatcaaaa atttctaaac
      601 agaagccata aaggttttca acaggtaacc gatacattag tttcaatgtt tactgagtaa
      661 ctaattattt tgcaggag
//
LOCUS       DAJSMI010000349          673 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJSMI010000349 DAJSMI010000000
VERSION     DAJSMI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.358"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(214..663)
                     /locus_tag="ON430_002901"
                     /pseudo
     CDS             complement(214..663)
                     /locus_tag="ON430_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          197 a          139 c          107 g          230 t
ORIGIN      
        1 ccgatccaaa ttcgacaaca gagattggcg aagcgaatcc ttgataatga gcgcggatct
       61 gatacctctg gagaggcagc gtaattgaat gattgtgctt ctgccggaga aaatcagatc
      121 cgaggatgct cattgtcaag ggcaatcgta gccataacac cgcagcattc acaacacctt
      181 ataatttttg tcaaaaaaag tgttgccatt ccactttttt aaatatgcgt tctctgctct
      241 taaacgaata acttcttctt caagagactc atctttaagt tttttaggaa tgtttagctt
      301 ggaattcata ctaatttttc tgcccctttt ttggctctca agtgaagaag caccgccttc
      361 ttcatattgc tctatccatt tacttagagt tctatttgaa ccgatattaa attttttagc
      421 agtttcttgg atagaaagac catttgtttc catatattct ataacatcaa gtttaaattt
      481 tgtagtgtag cttttgccac ctccaaccaa gccttcccaa ccatgatagt tataaatcct
      541 tacccaatgt ctaaccagtg tacgatttat ttgatattta tgtgcaagat atttgtagcc
      601 gccttcgtta tttaaatagt ctgaaactac ttttttctta aaaacaaatg tatatttccg
      661 caaaaaaagc acc
//
LOCUS       DAJSMI010000350          673 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJSMI010000350 DAJSMI010000000
VERSION     DAJSMI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.359"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..317
                     /locus_tag="ON430_002902"
     CDS             <1..317
                     /locus_tag="ON430_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR3220749.1"
                     /translation="EINSNDLATYILRYDDKIIEIHLDYFGRVPTRKFQLFTLDECIN
                     VDLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAE
                     EK"
     gene            318..>673
                     /locus_tag="ON430_002903"
     CDS             318..>673
                     /locus_tag="ON430_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR3220750.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLR"
BASE COUNT          262 a           72 c          123 g          216 t
ORIGIN      
        1 ttgaaattaa cagtaatgat ttagcaacat atatattgag atacgatgat aaaattatag
       61 aaattcattt agattatttt ggaagagttc ctacaagaaa atttcaattg tttactttag
      121 atgaatgtat caatgttgac ttattaaata ataaaataag ttttgaagaa aacattgtta
      181 aagaaatgcc tgaatctgct aatgatatgt atgagaagga gttgaaatat ttctttgaaa
      241 acgtagtaac aggcaaagaa aactggaatg atttatatca tgcaaacgaa gtattgaaaa
      301 tagctgagga gaaataaatg gaaattttat ttactatttg tggtagagct gggtctaagg
      361 ggtttaaaaa taaaaatttg aaatatttgg caggaaagcc cctagtccat ttcgctttag
      421 caatgataga tatctatcaa caaaaacatc ctgaacataa ttgcacgact tgtttgaata
      481 cggacagtaa tgagttagta gaattgacga ctaagttaaa tccgtctgtg aaaattgtag
      541 aacgtaaacc tgaattagct acagatactg tagggaaaat tgaagtgata cgtgattcat
      601 tcttacaact agaaagagaa gataatatat ttgatttaat tatagattta gatttgactt
      661 cacctttacg tga
//
LOCUS       DAJSMI010000351          667 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJSMI010000351 DAJSMI010000000
VERSION     DAJSMI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.360"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(32..>667)
                     /locus_tag="ON430_002904"
     CDS             complement(32..>667)
                     /locus_tag="ON430_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR3220751.1"
                     /translation="FKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNT
                     TAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDY
                     LNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHII
                     DPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          217 a          100 c           76 g          274 t
ORIGIN      
        1 taataaactc ttctctaaaa caggtactat tctattcatc tatatttcca actttcaaaa
       61 tttctttatc aattctattt aatagtttat cagagtaatc cgatttaact tttcttacaa
      121 ataatttttc actttctttt atttgatcta tatccctttc atcaaaccat ttgttcatgg
      181 aaggatcaat aatatgaata tttgctaatt tcgaagtttg taaagtagtt tcttcaatat
      241 ttttcaattc aagtggtaaa tctggtcctc caataacatt tttatttttg aacgaggaat
      301 taaaaaaaag tgtatgaaaa aatttttcat ctggggcaaa catacctatt ttaaaattca
      361 ggtagtcttg ttgattttgt tcatacattt gtattaattc tgttaggcat tctttcgaca
      421 aagcccacca ttgagaacca tgatacaaat caaatttatg gtgtctaatt tttgggggaa
      481 gtttaatcat cctcagacaa cgattaatta tagctctaaa aattttaaat gcagttgtgt
      541 tattcctatt aattctagga taatcatgtt tttgatatac atctatatgt ttataataaa
      601 attctttatg tttaatttga tctagatcta ttccacgaat aaactccact gaactattct
      661 ttttaaa
//
LOCUS       DAJSMI010000352          667 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJSMI010000352 DAJSMI010000000
VERSION     DAJSMI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.361"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..437)
                     /locus_tag="ON430_002905"
     CDS             complement(<1..437)
                     /locus_tag="ON430_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3220752.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT          201 a          115 c           90 g          261 t
ORIGIN      
        1 ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa tactttataa
       61 gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat atatttttcc
      121 tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg agataacaga
      181 gctttgctag aacattttcc attaatttcc agacatatat ccaattcact gacggttact
      241 cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc gatcgttttt
      301 ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga ctctgataaa
      361 tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc tttgcttaaa
      421 aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc catgcattca
      481 ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc agcgattctt
      541 gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg ttttatttgg
      601 cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg catttttatt
      661 aataaaa
//
LOCUS       DAJSMI010000353          660 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJSMI010000353 DAJSMI010000000
VERSION     DAJSMI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.362"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..630
                     /locus_tag="ON430_002906"
     CDS             <1..630
                     /locus_tag="ON430_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCR3220753.1"
                     /translation="VGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEYGKLLYQI
                     WKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKR
                     LVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRN
                     KFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          227 a          117 c          129 g          187 t
ORIGIN      
        1 gtgggctact atcttcgtta taaccttagc tatcgtgaag ttcaagaaat cttatatgat
       61 cgtggcatta acgtttctca tacgacgatt tatcgttggg tgcaagaata tggcaaacta
      121 ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt attcatggaa aatggatgaa
      181 acgtacatca aaattaaagg aaaatggcat tatttgtatc gagccatcga tgcagatggt
      241 ttaaccttgg atatttggtt acgtaaaaaa cgggacacac aagcagccta tgcttttctt
      301 aagcggttag tgaagcagtt tgatgaaccg aaggttgtag tcacagataa agccccctct
      361 attacaagtg cctttaagaa actaaaagaa tacggctttt atcaagggac agaacatcgt
      421 accattaaat acctgaataa tttgattgaa caagaccatc gtccagtaaa gagacgcaat
      481 aaattctatc gaagtttacg cactgcctct accacgatta aaggcatgga agccattcga
      541 ggattatata agaaaacccg aaaagaaggc actctcttcg ggttttcggt ctgtactgaa
      601 atcaaggtat tattgggaat cccagcttaa atcatagata ccgtaaggga ttttattctt
//
LOCUS       DAJSMI010000354          650 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJSMI010000354 DAJSMI010000000
VERSION     DAJSMI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.363"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>650
                     /locus_tag="ON430_002907"
     CDS             <1..>650
                     /locus_tag="ON430_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220754.1"
                     /translation="IFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKA
                     AGRKMMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYI
                     VPKGKRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEP
                     ARYDRHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPE"
BASE COUNT          259 a          104 c          134 g          153 t
ORIGIN      
        1 atcttccaaa gaggacaaca aaaaggctca atcatttatc aagatgttgt ttctcatgat
       61 aattcgtatc ttgaaaaaat aggattgtac gatcccaaaa cagatacttt agatgaagaa
      121 gggttaaaag cagctggaag aaaaatgatg gagacattat tcgagaaaga gcaactgaac
      181 gaaacaggct actggatggc aacgattcac cgtaacacta aacatatcca tattcatttt
      241 gcagttgttg aaaaagaaaa tacgcgtaaa ccttacacgg aggttgaaaa tggtgtgagt
      301 tatatcgtac cgaaaggtaa gagaagacaa gcaacattag acgcaatgaa gaccaattat
      361 gtacatgagt tagaacgttt ttcttatgaa aaggatcgaa tgattggtat ggagaaacga
      421 aatttattga cgcggaaaag tgatttgcgt aacaccttaa caaaagtggt aaaagagcca
      481 gcgcgttacg atcgacacgc gatgaactta ttaaatgaag tatataaaac cctacctgac
      541 aaacgttcgg aatggaatta cggggatgaa aaacgaacca aactttcacc atacacaaga
      601 gagaaactcg atgagttaac caaatatgtt ttaacaaaag aacctgaata
//
LOCUS       DAJSMI010000355          650 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJSMI010000355 DAJSMI010000000
VERSION     DAJSMI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.364"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>650
                     /locus_tag="ON430_002908"
     CDS             <1..>650
                     /locus_tag="ON430_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220755.1"
                     /translation="IFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEVGLKA
                     AGRKMMETLFEKEKLNDTGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYI
                     VPKGKRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEP
                     VRYDRHAMNLLNEVYKTLPDKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPE"
BASE COUNT          256 a           98 c          135 g          161 t
ORIGIN      
        1 atcttccaaa gagggcaaca aaaaggctca atcatttatc aagatgttgt ttctcatgat
       61 aattcgtatc ttgaaaaaat aggattgtac gatcccaaaa cagatacttt agatgaagtt
      121 ggactaaaag ctgctggaag gaaaatgatg gagacgttgt ttgagaaaga aaaactgaat
      181 gatacgggat attggatggc taccattcac cgtaacacta agcatattca tattcatttt
      241 gcagttgttg aaaaagaaaa tacgcgtaaa ccatacactg aggtcgaaaa tggtgtgagt
      301 tatatcgtac cgaaaggaaa gagaagacaa gcaacattag acgcaatgaa gaccaattat
      361 gtgcatgaat tagaacgttt ttcttatgaa aaggatcgaa tgataggcat ggagaaacga
      421 aatttattaa cgcggaaaag tgatttgcgt aacagcttaa caaaagtggt aaaagagcca
      481 gtacgttatg atcgacacgc aatgaactta ttaaatgaag tatataaaac cttacctgac
      541 aaacgttcgg aatggaatta tggggatgaa aatcgaacta aactttcacc atacacaaga
      601 gagaaactcg acgagttaac ccaatatgtg ttaacaaaag aacctgaata
//
LOCUS       DAJSMI010000356          649 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJSMI010000356 DAJSMI010000000
VERSION     DAJSMI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.365"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>649
                     /locus_tag="ON430_002909"
     CDS             <1..>649
                     /locus_tag="ON430_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3220756.1"
                     /translation="RQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELAKKNKV
                     KKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGTTILYA
                     LPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSFESLNV
                     DISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTFRKL"
BASE COUNT          248 a          102 c          109 g          190 t
ORIGIN      
        1 aagacaagaa caaatcatta cattattaga gaaagatttt tatttaacta attcagaaat
       61 cgctaacaaa ttaggcgtat cagaaatgac catacgccga gatgtcactg aattagcaaa
      121 aaaaaataaa gtaaaaaagc tatatggtgg cgttgaacga aaggaacctt ataatcgaga
      181 actatcgact caagaaaaga ttgatacgaa tgtagccgaa aaaaaatata ttggtcaagt
      241 catgaacagt atgattcccg ataattctac tatttatgtc ggtgctggaa caacgatcct
      301 atatgcactt cctttcattc aaaagaaaaa tctatttgta attacaaaca gtttgattgc
      361 tttcaactat ttaaagaatc atacaagtta tcggatttta cttactggtg gcgaattttc
      421 tcctattaca gaagagttca ttggggaagt tgcagaaaga tcttttgaaa gtttgaatgt
      481 cgatatctct ttcgctgcaa caaacggcat atttgataac aatgtgacaa ccgctcaatt
      541 tattgaggga aatattcaga acgcagcgct caatgcagca aaaatcaaat gcattgttgc
      601 agattattca aaattaaata aatcagacgt ctatactttt agaaaatta
//
LOCUS       DAJSMI010000357          643 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJSMI010000357 DAJSMI010000000
VERSION     DAJSMI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.366"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(82..636)
                     /locus_tag="ON430_002910"
     CDS             complement(82..636)
                     /locus_tag="ON430_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3220757.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          191 a          135 c           66 g          251 t
ORIGIN      
        1 acttccgaaa attcagttat cttcattaat ctcaccttct aagttttcat tatacacaaa
       61 aaatatatcc taatatctca attagcctcc caatttaaaa ccacgtaacg atgcccctat
      121 ttcatcttca gttataccaa tgtacctttt tgtaattgct tgcgagctat gattaaaaat
      181 aaacattaag gtagcaatgt ctctagtttt cttgtagtag tgatacccaa aagtttttct
      241 taatgtatgt gttcctatat catctcttcc taatttttct gctatatctg catatatctg
      301 atagacacga tcccttgtta tatgtcgacc ttgttgccga cttggaaata accaatcatt
      361 ttcttcttta ccttcagtat ataataaaat ctcttctctc aaattatcta taaaaatcgg
      421 ttttcgctta cctgttttat gttcaacaat aataggctta cttttacctc gtatgtctcc
      481 aacttttaat cctataatat ctgacatacg taacccatta ttgattccaa acaaaaataa
      541 aaaactatct ctttctggat tacttgttct tcttagccaa aactgaaaat cttgtatctc
      601 ttcatttgtc cttaacggtt gtacattata gctcactatt gtt
//
LOCUS       DAJSMI010000358          639 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJSMI010000358 DAJSMI010000000
VERSION     DAJSMI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.367"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            110..631
                     /locus_tag="ON430_002911"
     CDS             110..631
                     /locus_tag="ON430_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR3220758.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT          242 a          108 c          123 g          166 t
ORIGIN      
        1 tttacatgta tctatttgaa ttggtcatct aatttttaga caaacagtct aaatcgttgc
       61 ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca tggatatatc
      121 agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt catcgaatgc
      181 tcctaaaaaa atggtcgaat tcatcaactt agcttgtccg tattgccgac aatggtttga
      241 agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc gagtgatcaa
      301 attgttcgat aaagaaaaag aaagcttaca gcgagggaac gtgatgcacc ggtatctcac
      361 aatcagcgat ggccaaaaag cgatcaaaga gatcaaacaa atattcgata ctcaagacga
      421 gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac taaagctgac
      481 agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc aagcacatat
      541 tcgatttgtt ccaactgtca tcttgggtaa agaaatcttt gatgaatcta tttcaataga
      601 ggaattaaaa gaattgatcc aaacaaaata aaaaaataa
//
LOCUS       DAJSMI010000359          628 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJSMI010000359 DAJSMI010000000
VERSION     DAJSMI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.368"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            82..>628
                     /locus_tag="ON430_002912"
     CDS             82..>628
                     /locus_tag="ON430_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3220759.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSLL"
BASE COUNT          215 a          124 c          139 g          150 t
ORIGIN      
        1 agcaacaaga aattagaaag caaaaacgtg cgatgttgtt tcaacaaggc agacaacaag
       61 aaagggagtg agatcgtcac gatgaaaact gtcattttag cagaaaaacc gtcacaagcg
      121 aaggcttatg ccgatagctt ttctaaagcc acccgaaaag acgggtattt tgagatccaa
      181 gatcgattgt ttcctggtga aactgtgatt acgtatggtt ttggtcattt agtcgagtta
      241 gattcgcccg atatgtacga tgaaaattgg aaacaatggt cattagaaca tttacctatt
      301 ttccctacac attatcacta tcatgttcct aaagataaga aaaagcaatt caacgtggtg
      361 aaacagcaac ttcaatcggc cgacaccatt attattgcga cagatagcga tcgcgaaggg
      421 gaactgatcg cttggacgat cattcaacaa gcaggcgccg atcatggaaa gatatttaaa
      481 agactttgga tcaactcctt agaaaaagaa gccatttatc agggattcca gcagttacga
      541 gatgcagaag agacctaccc aaaatttgaa gaagcacaag cacgccagat cgccgattgg
      601 ttgatcggca tgaacggtag cctactgt
//
LOCUS       DAJSMI010000360          621 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJSMI010000360 DAJSMI010000000
VERSION     DAJSMI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.369"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>621
                     /locus_tag="ON430_002913"
     CDS             <1..>621
                     /locus_tag="ON430_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3220760.1"
                     /translation="LGLEKEFGNRGKKKLTNYIISRTKMLNFDSILNQIKSFVLSSFN
                     IILFSTGVYLITLNQLSSGVLLTFNALLAYVTNPILNLANLQSVLVQGKVAQEKLYNI
                     LESQIVLYGNKSLVIENDDIFINFDLVSYDYDSNSKILKDISINICGNNIALSGTNGV
                     GKSTIGKLIARLYIPDSGSIEINGQNLIDISEESVSKNIIYVDGRE"
BASE COUNT          225 a           82 c          100 g          214 t
ORIGIN      
        1 actgggactt gaaaaagaat ttggtaatag aggtaaaaaa aagcttacca attatattat
       61 ttctcgaaca aaaatgttga attttgactc gattctaaat caaataaagt cttttgtact
      121 atcgtcattc aatattattt tattttcaac tggtgtatat ttgattactt taaatcagtt
      181 atcttcaggt gtcctgctta cattcaatgc tctactagca tacgtaacta atcctatttt
      241 gaatcttgct aacttacagt cggtgttagt acaaggaaaa gtagcacaag agaagcttta
      301 taatattcta gaatcacaaa tagttttata tggaaataag tcattggtaa tagaaaatga
      361 cgatattttt atcaactttg atttagtatc atatgattat gattccaact ctaaaatttt
      421 aaaagatatc tcaattaata tatgcggaaa caacatcgca ttaagcggaa ctaatggagt
      481 tggtaagtct acaataggaa agcttattgc tcgtctctat attccagata gtggaagtat
      541 agaaatcaat gggcaaaact tgattgacat atcagaagag tctgtttcta aaaatattat
      601 ttatgtagat ggtagagaaa g
//
LOCUS       DAJSMI010000361          611 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJSMI010000361 DAJSMI010000000
VERSION     DAJSMI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.370"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(117..>611)
                     /locus_tag="ON430_002914"
     CDS             complement(117..>611)
                     /locus_tag="ON430_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220761.1"
                     /translation="SYLIVCLIIIICLHHLFLSKTIPRYSKNKKAEKFCLLLNKFTLI
                     APILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCL
                     VINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFY
                     RNKL"
BASE COUNT          249 a           69 c           94 g          199 t
ORIGIN      
        1 ctttaattgc aaataaatgt atgaattatc ttgttattat ggagaaaagc tgtttcgttt
       61 atcttttttg catgaaaaag taaatgtaca tactcagtag ctaggatggt aacttattac
      121 aatttattgc gataaaaata aaagttgtta tttttattgg gaaaataccc gatgtagaaa
      181 ataatgcctt ccaatatacc tataaaataa gtccagtatt ccaaaggatt atataaataa
      241 gtgacgtatc tatctaaagg tgtaataaaa atgataagtg ataataaaaa aataccattt
      301 ataactaaac ataaactaat ggatttgggc ggtttcaggc tcatcagaaa aatatagatt
      361 ctggtgaaca acaaccataa aaaagtgaga atcattgcat gagagcttct ttccataaat
      421 tttcctttaa gagtagtgct taaaagtaca gaaaaaataa taaatgcaag gataggagca
      481 attaaagtaa atttatttaa taataaacaa aatttttcag cttttttatt cttggaataa
      541 cgaggtattg ttttcgataa aaaaagatgg tgcagacaaa ttatgataat taagcatact
      601 atcagataac t
//
LOCUS       DAJSMI010000362          606 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJSMI010000362 DAJSMI010000000
VERSION     DAJSMI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.371"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            16..246
                     /locus_tag="ON430_002915"
     CDS             16..246
                     /locus_tag="ON430_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3220762.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            246..587
                     /locus_tag="ON430_002916"
     CDS             246..587
                     /locus_tag="ON430_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3220763.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
BASE COUNT          231 a           85 c          115 g          175 t
ORIGIN      
        1 aagggagtga tgataatgct tgttacaaaa tctagattac aaggaagttc tgtagtggta
       61 actttacctt ctgataacgg aaagaaacct tcagaaaacc aagaatatat cgttgtgtat
      121 tctgatgatg gaacaattac gttagttcca aaaattgaag atccatttag tggtggggaa
      181 gaggccgagt attatgagaa agacgaatgg gaagatttaa ctcctgaagg gagagaaatt
      241 ttatagtgtc agaagaaaga atttatattc ctaaaaaagg tgatattgta tggattgatt
      301 ttgatccctc cgtaggaaaa gaaattcaaa aaagacgacc aggactagtc gtctctcgtt
      361 atgaatttaa ccgtaaaaca atgttttcgg tcatatgtcc aattacttcc actataaaga
      421 atttaccgac acgttactca ttacctgaag agctagatat aaaaggacaa gtaattatta
      481 cacaattaaa atctcttgat tttaaggaac gaaaactcaa aaaaatagaa aacttaccat
      541 tgcaagacat ggctaaaatt gatcagatta tacagtatat gttttaagca ttgtaagtaa
      601 actcta
//
LOCUS       DAJSMI010000363          601 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJSMI010000363 DAJSMI010000000
VERSION     DAJSMI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.372"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..545)
                     /locus_tag="ON430_002917"
     CDS             complement(<1..545)
                     /locus_tag="ON430_002917"
                     /inference="COORDINATES: protein motif:HMM:NF025388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HCR3220764.1"
                     /translation="MIKTNEWPSNEAALLSHIALTLIRSPYIKKLFYDNDNIPLFMKS
                     IMYRQIIGSQMMAVEHAKSHLNGEELEIALHVLKGNSDSGIQVILEHLMNNYQLEIYM
                     SEGKGNFFLGDNPILVNKFEDIDYLIPIAPSLCLGTTKLRLQNNRVLIKSNFNIVDDN
                     MVRKINHLTLRNARQTVIVST"
BASE COUNT          184 a           92 c           85 g          240 t
ORIGIN      
        1 tttgtggata caattacagt ttgtcttgca tttcgtagtg tcaaatgatt aatttttctc
       61 accatattat cgtcgactat attgaagttt gatttgataa gtacacgatt attttgtaat
      121 cttaattttg ttgttcctaa acataggctg ggagcaattg gtattaaata atcaatatct
      181 tcaaatttat taactaatat tggattatct cctaaaaaaa aatttccctt cccttccgac
      241 atgtatattt caagttgata attgttcatt aaatgttcta agataacttg gattccacta
      301 tcgctatttc cttttaaaac gtgtaatgcg atttctaatt cttcaccatt taaatggctt
      361 tttgcatgtt ctacagccat catttgagat ccaataatct gtctatacat tattgacttc
      421 atgaataatg gaatattgtc attatcgtaa aacaattttt ttatataagg gctacgtatt
      481 agtgttaagg ctatatgact aagtagggct gcttcattac ttggccactc atttgtttta
      541 atcatacgca gaaaatctga ggttgattta ttcgaaagta acaattctat taatgactta
      601 a
//
LOCUS       DAJSMI010000364          585 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJSMI010000364 DAJSMI010000000
VERSION     DAJSMI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.373"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            153..585
                     /locus_tag="ON430_002918"
                     /pseudo
     CDS             153..585
                     /locus_tag="ON430_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          212 a           71 c           92 g          210 t
ORIGIN      
        1 tcttaaatat tatgttggta catgaaaata tacatccctg ctagactatt tacaacattt
       61 tactttgtac gtattcttgt tttaaaagga atgggataaa atataggtac aataactatt
      121 tgatgctaaa tataattaat ggaggagaga tagtgaacat acttaatgaa aaaagatatg
      181 tatggtttgc tggtaaaaaa tatattacta ctttcaaaag attatccgag ttaatgcagc
      241 agactgttaa aagtgctatg cctgaaggat acaagtttaa ttttgtttaa tcttcagtat
      301 ctactgaaag tatctatttt tcaattcttt ctgagagtag gctttttcat tttagaataa
      361 gttctcatgt aaagaaaact gatgttgata tatatacgtt ccgtacaaat aattataaaa
      421 atttaaatga tttgagtaga agtatcagtg aatatctcaa ggggaatagc tatctcaaga
      481 ttcgattttc acatatatta attttactac catatttgaa tgagaatttc accctaattc
      541 aagtagataa gtctttcatt gcctcaggta atcaaggaaa tatga
//
LOCUS       DAJSMI010000365          575 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJSMI010000365 DAJSMI010000000
VERSION     DAJSMI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.374"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(37..>575)
                     /locus_tag="ON430_002919"
     CDS             complement(37..>575)
                     /locus_tag="ON430_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220765.1"
                     /translation="KLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKE
                     LLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTE
                     VIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDS
                     KIDKQIDDFPEEIIDRIE"
BASE COUNT          187 a           98 c           92 g          198 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaa
//
LOCUS       DAJSMI010000366          567 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJSMI010000366 DAJSMI010000000
VERSION     DAJSMI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.375"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..175
                     /locus_tag="ON430_002920"
     CDS             <1..175
                     /locus_tag="ON430_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3220766.1"
                     /translation="YKGRPKLYSADTKNPQRRLVYENIVKDLNEGVSISKIAKNYNVT
                     RQTIYRIKKDLNL"
     gene            complement(170..>567)
                     /locus_tag="ON430_002921"
     CDS             complement(170..>567)
                     /locus_tag="ON430_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220767.1"
                     /translation="KNKDEKLFVIRPDLMKSNTHYIKEKPKDCDYIIISEANKTVFLI
                     ELKSSAQTSPAKEISEQIDGGYKWLQHLSFLTKYDFTEFSYKIIKVCCKFEARQDRKN
                     LFKPNEYGIYNMNGPKLDLRELYRKCNDL"
BASE COUNT          188 a           88 c           74 g          217 t
ORIGIN      
        1 ttataaaggt cgtccaaaac tctatagtgc agatacaaaa aatcctcaac gacgtttagt
       61 ttatgaaaac atcgttaagg acttgaatga gggcgtctct atttcaaaga ttgcaaagaa
      121 ttataacgtt actagacaaa caatatatag aattaaaaaa gatttgaatt tatagatcgt
      181 tacactttct atacaactct cttaagtcta attttggacc attcatatta taaataccat
      241 attcattagg tttaaaaaga tttttacgat cttgtcttgc ctcaaattta caacatacct
      301 taataatttt ataactgaat tcagtaaaat catattttgt tagaaaacta agatgttgta
      361 accacttata tccaccatca atttgttcac taatttcttt tgcaggggaa gtctgagctg
      421 agcttttaag ttctatcaga aatactgttt tatttgcttc acttatgatg atgtagtcac
      481 aatctttagg tttttctttt atatagtggg tattactctt cataagatct ggtcttatta
      541 cgaacaattt ttcatcttta tttttct
//
LOCUS       DAJSMI010000367          565 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJSMI010000367 DAJSMI010000000
VERSION     DAJSMI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.376"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            104..>565
                     /locus_tag="ON430_002922"
     CDS             104..>565
                     /locus_tag="ON430_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220768.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPAD"
BASE COUNT          197 a           77 c          126 g          165 t
ORIGIN      
        1 atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt aaagcagata
       61 aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa agatttttgg
      121 tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg ctgctgagaa
      181 agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt ttgaagaaga
      241 agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc
      301 tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc ctgcttcagt
      361 ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata ttgaaactac
      421 tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac accttcttgt
      481 actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat ggcgttttgg
      541 aggtatgggt gtaaaaccag ctgat
//
LOCUS       DAJSMI010000368          558 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJSMI010000368 DAJSMI010000000
VERSION     DAJSMI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.377"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(127..522)
                     /locus_tag="ON430_002923"
     CDS             complement(127..522)
                     /locus_tag="ON430_002923"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR3220769.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
BASE COUNT          175 a          115 c           44 g          224 t
ORIGIN      
        1 ataaaatgga ctttgtctac agtctgtatc ttctcttttt gtgtctactt tattgacatc
       61 tatccaatta gtaatcaata aatatttcaa taatagtcta gctatatata aataacatta
      121 caaaacctaa agaattatat ctgataacct tccatgtttt tcatatgatg ttactctttc
      181 aactttatta ttatcatcga caaaaacaac cttaggtcta tgatccttaa cctcattttc
      241 atttaattca caatatgcca ttaaaattat tttatctcca gttgatacac atctagctgc
      301 agctccattc aagcaaatca tccctgttcc tctctctcca gctattgtat aagtttcaaa
      361 tctatttcca ttattaacat caactatttg tactttttca tattcatata ttcctgctgc
      421 ttctaataaa ttcatatcaa cagtaatact tcctacataa tctaactcag cttgttcaac
      481 tgtagctcta tgtatttttc cttttaacat tgtaactttc attatagccc cctattgtaa
      541 ttcaaatgta aaattatc
//
LOCUS       DAJSMI010000369          544 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJSMI010000369 DAJSMI010000000
VERSION     DAJSMI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.378"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(251..544)
                     /locus_tag="ON430_002924"
                     /pseudo
     CDS             complement(251..544)
                     /locus_tag="ON430_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          172 a           91 c           96 g          185 t
ORIGIN      
        1 cggtcttaca ttcataaacg aaaaaaggct cttcgacatt tagtgttggt agagaaagat
       61 acaactgaaa atttagtcat aaccagttag gcagagtgct taactggttt ttgttttact
      121 tggaagatca agccttgaat gagataaatt cttgctcgaa agttatggaa atttcgatag
      181 ccgtaggcta cttgtattgt gcaatagttt tttacaaaaa aaaggggtgc ggatattttt
      241 ttggaccttt ttaacaaata tatctttgct tcttccgata ttgaagaggg cttagccctc
      301 ctaaagataa cttaattcgt tgattattat accaatgaat ataattatcc aatttgttta
      361 taaactgatt gatcgttgta ttcttccagt cacgttgata aaacatctcg ttttttaatc
      421 gtccaaagaa accttcgcac gctgaattat ctggtgaata acccttttta gacatagagt
      481 gaagcaagtt gtctttgttc caatagtcca actaacaata gcaccatcgt aacaatcgac
      541 aagt
//
LOCUS       DAJSMI010000370          519 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJSMI010000370 DAJSMI010000000
VERSION     DAJSMI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.379"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>519
                     /locus_tag="ON430_002925"
     CDS             <1..>519
                     /locus_tag="ON430_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR3220770.1"
                     /translation="IIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSS
                     NLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKK
                     SYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIH
                     EKLALKGITISV"
BASE COUNT          168 a           97 c           92 g          162 t
ORIGIN      
        1 tgattattgc ttgtgttata tggggcataa ttaatggcta tactagccaa agagccacgt
       61 atagagcggt ttgttccgtc ttatttccta atggtgactt tcctagtagg agtcgattca
      121 ctcgcttaag ttcaaactta gcttacacta tcaagattat tcgatacttt ttcatcaaga
      181 agctcaccaa aggtgaacta gtcggaatta tagatagttt tcctagtcca ttatgtaaac
      241 cagttagaaa tagacaagca aaactattga atcaaatagc caaagttggc tataactcaa
      301 caaaaaaatc ttatttttat ggtcttaaaa ttcatatgat cgttactaag actggctttc
      361 cgattactta ctcaatcacg aatccaggtg tccatgacgt gaaagtgtta gagactttat
      421 cggaagaagc gaatcttccc aatatcctag gggacaaagg atatattagc cataaaatcc
      481 atgaaaagct tgcccttaag ggcattacta tatccgttc
//
LOCUS       DAJSMI010000371          514 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJSMI010000371 DAJSMI010000000
VERSION     DAJSMI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.380"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..132)
                     /locus_tag="ON430_002926"
     CDS             complement(<1..132)
                     /locus_tag="ON430_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3220771.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAJSMI010000372          502 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJSMI010000372 DAJSMI010000000
VERSION     DAJSMI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.381"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..418)
                     /locus_tag="ON430_002927"
     CDS             complement(<1..418)
                     /locus_tag="ON430_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220772.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIH"
BASE COUNT          162 a           82 c           73 g          185 t
ORIGIN      
        1 aatgtatttt ggttaactct cttcggatca attggctcgt ctcttctcgc aacagattaa
       61 actgattttc aaaactttca atgaatcgtt cattcaacca ttcgattgtt tcatcttcat
      121 tttttctcaa gtatttttga tccaatttat catgaagata aagccaagag agtgtcttat
      181 tgataaaaat aacggatttt tctcctaata ctccttttaa gtggtaaaat cctatatccg
      241 aaaaaaatag cgactgattg ctagcaatat ctaaagaatc agcttgttca gccaacaggt
      301 tgattgctaa aaagtggaat aatttctttt tttcataagc agacagagga taatactgtt
      361 ttaaatcttt taacattttt tctgttttta taattatagg tagcgtatac aattccacaa
      421 aacttatctc tgttgagtta aaaagattat ataaaaaaag ccgaatgttt ttaggattcc
      481 ctgtcaactt gggagcgtca at
//
LOCUS       DAJSMI010000373          502 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJSMI010000373 DAJSMI010000000
VERSION     DAJSMI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.382"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            32..>502
                     /locus_tag="ON430_002928"
     CDS             32..>502
                     /locus_tag="ON430_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCR3220773.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKK"
BASE COUNT          184 a           67 c           88 g          163 t
ORIGIN      
        1 gtaagaatgg gacttgtagt ctcacttctt tatggaggtg ataattatga acgttggttt
       61 tattggagcc ggcaaggtag gttgttcatt cgggaagtat ttccaggaac ataaaataca
      121 agtaacagga ttttatagta aaagcgaaga ctcgtcatta gcagcttcga attttacatc
      181 tagtaaacaa tatttaaact taagagaatt agtagatgaa aatgatacta ttttcattac
      241 gactcctgat ggtcaaattc aggaggtatg gcaagaaatt aaaaattatc aaatcaaaaa
      301 caaattaatc tgccattgca gtggtgcaat atcttcagat atcttttcaa atatacagga
      361 ctatggtgct tatggctatt ctgtccatcc tatgttttca atatcagata aatataattc
      421 atacaaaaaa ttaaaaaaat cttttataac aattgaaggc gatgaaaaat atgcaaagtt
      481 tttatgtgat ttcttcaaaa ag
//
LOCUS       DAJSMI010000374          501 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJSMI010000374 DAJSMI010000000
VERSION     DAJSMI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.383"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            75..269
                     /locus_tag="ON430_002929"
     CDS             75..269
                     /locus_tag="ON430_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220774.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          214 a           64 c           85 g          138 t
ORIGIN      
        1 atatagatac ataatcattt tcacttacat atatttgaat attaactaaa aacagcaaga
       61 aagaagagga tcaaatgtat tatgtagaag taaaaacaaa aggcgtcaaa aacaaacaat
      121 atgtgaaagg tatcagtaac gaatatcctt tacttggttc atggaaagaa gctgctcctt
      181 tttccaaacc atgtgccata aaaataaaga acgagctaga aaaagaactg acttgtggta
      241 aagctgttgt agatatcatc gaaaagtaaa tccaatgtat agaaaggaag atcacacatg
      301 aaaagcgacg tacaaattgc taagattata gaattagaag aagaagacgc agtgttgaca
      361 attgaaaaaa aataaatgtt tgtataccat atgattattt caatatgtat cctggaattg
      421 gagatctcgt agagctatat caagatgaat caaatatact tgtcattcat catgaaatat
      481 agagacttat aaaattgttt a
//
LOCUS       DAJSMI010000375          491 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJSMI010000375 DAJSMI010000000
VERSION     DAJSMI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.385"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(111..410)
                     /locus_tag="ON430_002930"
     CDS             complement(111..410)
                     /locus_tag="ON430_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR3220775.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          150 a           96 c           58 g          187 t
ORIGIN      
        1 tgtttcttta tttagaataa aaaaatacag actctgttca aataaatgaa cctagcctgc
       61 attatgagat tgactatact ttttaattca ttgagatgaa taactcgttt ttataaatgc
      121 ccaaaagtaa tcggtgccga agccattcct cgacctaatt ctttttcaaa ttttcctatt
      181 gttgatatct ccatataaaa cggtgatact ggtttagaca attctcccct gattgctaac
      241 ggacgtaaac tacgttccaa attatttatg actttaggat aatactgacc tttttctaag
      301 cctgtctttg cgtctatcaa aagtatacgt tccttttctt tgatctctgg atttaaaacg
      361 agatcatata attttagtac catgttttct atttcttcac ttttagacat cacgatttcc
      421 tcccaaaaat atatttcttc ccactacatt ctataaacaa agtatacgct acatctagat
      481 ttttttaaat c
//
LOCUS       DAJSMI010000376          487 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJSMI010000376 DAJSMI010000000
VERSION     DAJSMI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.386"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(<1..436)
                     /locus_tag="ON430_002931"
     CDS             complement(<1..436)
                     /locus_tag="ON430_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3220776.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIY"
BASE COUNT          138 a           94 c           61 g          194 t
ORIGIN      
        1 cgtatatgtt ttgcggtgtt tcttgtttct tgtaattttc cacgaatgtt tcatccatga
       61 ataaattcat aaacgcttga agtaatccta cttgttcttt tggttctttg attcctaaaa
      121 aaaatagatt ttctacagtc atttcctgat tatcccccat ttgataaaaa taaattgggt
      181 tcttcagctg cataacaaaa acaaatggct tttcgacata ttcacattcc gaatgtggca
      241 gcgcgatatt taaatactta gtaatcaatc cagtaggaaa ttctctttct cttgcaatca
      301 gtccattcaa ataattctca ttgacgtacc ccattttttc taattgttgt gcagcgatta
      361 aaaaagcttc ttctttattt tttacttcta ccgctaattg aatcaaacta ggaacgaaca
      421 tctctttata tttcattcca acctcctcct ttttgattaa gtttgttaat ttattgttct
      481 actaagt
//
LOCUS       DAJSMI010000377          487 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJSMI010000377 DAJSMI010000000
VERSION     DAJSMI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.387"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(94..165)
                     /locus_tag="ON430_002932"
     tRNA            complement(94..165)
                     /locus_tag="ON430_002932"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Glu,seq:ttc)
     gene            complement(177..250)
                     /locus_tag="ON430_002933"
     tRNA            complement(177..250)
                     /locus_tag="ON430_002933"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(214..216),aa:Ile,seq:gat)
     gene            complement(263..333)
                     /locus_tag="ON430_002934"
     tRNA            complement(263..333)
                     /locus_tag="ON430_002934"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(299..301),aa:Gly,seq:tcc)
     gene            complement(338..410)
                     /locus_tag="ON430_002935"
     tRNA            complement(338..410)
                     /locus_tag="ON430_002935"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(375..377),aa:Phe,seq:gaa)
     gene            complement(414..486)
                     /locus_tag="ON430_002936"
     tRNA            complement(414..486)
                     /locus_tag="ON430_002936"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Asp,seq:gtc)
BASE COUNT          152 a          133 c          104 g           98 t
ORIGIN      
        1 cttagcacaa tcatccataa agataactct gctaagcttt ttaaaatcat tctttcagat
       61 aaaaagaaaa agatactcaa aaagtatctt ccatgacccg tacgggattc gaacccgtgt
      121 taccgccgtg aaagggcggt gtcttaaccg cttgaccaac gggccaataa aataaatggg
      181 cctaaatgga ctcgaaccat cgacctcacg cttatcaggc gtgcgctcta accagctgag
      241 ctataggccc ataaaaacta aaagcgggtg acgagaatcg aactcgcgac aacagcttgg
      301 aaggctgtgg ttttaccact aaactacacc cgcataatgg cgcgggacag aatcgaactg
      361 ccgacacatg gagcttcaat ccattgctct accaactgag ctaccgagcc aaacggttcc
      421 gacgggaatc gaacccgcga tctcctgcgt gacaggcagg catgttaacc cctacaccac
      481 ggaacca
//
LOCUS       DAJSMI010000378          486 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJSMI010000378 DAJSMI010000000
VERSION     DAJSMI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.388"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
BASE COUNT          166 a           65 c           82 g          173 t
ORIGIN      
        1 ataacgtgat tttttgctgt aatgacaagg ttttagctgt ttcagatacc atgtatccaa
       61 aaatagttta ttgagaaaat aggagagtat ttgatactaa ctagtaggaa aaaatgagat
      121 aaaaagtaaa tggaatcatt taaggtaatc tgaaataaaa aatatacagc cctgtggtac
      181 tttacctaca aggctgtata tattttttac tctcagcgtt tttgactccg tgtctgactc
      241 cgttttattg attttgaatg ttattcaatg accatttaac aggtcaaatt tgatgttttt
      301 gattccgtat gacgccgttt gatatctagt gatatagtga tttgagtcta catcgaataa
      361 gggaataaac caataaaaaa tgatttagaa tattaaaata acaaaaaaga ggactacctg
      421 tattagtcct cttttttgac cagattttga ccagtctatt ttacatccta gtatatttat
      481 atgagc
//
LOCUS       DAJSMI010000379          479 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJSMI010000379 DAJSMI010000000
VERSION     DAJSMI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.389"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            7..>479
                     /locus_tag="ON430_002937"
     CDS             7..>479
                     /locus_tag="ON430_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HCR3220777.1"
                     /translation="MQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGF
                     NSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEK
                     GVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          174 a           87 c           80 g          138 t
ORIGIN      
        1 gaaattgtgc aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta
       61 gaaaaagcga taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa
      121 tatgatcgca ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc
      181 aaaaccgaat atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa
      241 caacaaactt taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat
      301 ttattcgaaa aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt
      361 catcactata ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat
      421 gcctttaaat ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctactta
//
LOCUS       DAJSMI010000380          465 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJSMI010000380 DAJSMI010000000
VERSION     DAJSMI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.390"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            51..>465
                     /locus_tag="ON430_002938"
     CDS             51..>465
                     /locus_tag="ON430_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR3220778.1"
                     /translation="MNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVINRKITPS
                     GYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHYDEILYLM
                     SMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYL"
BASE COUNT          192 a           67 c           73 g          133 t
ORIGIN      
        1 ttaaaaataa tgaaatctat tcctcagata tttttttcct agacatcgat ttgaacgcct
       61 attatagcgg tatcgaacta ggcgaaatgc ttagagaaaa gaatgaaaag tgtaaaattg
      121 tttatctaac gtctcatccg aataaagcaa taactgttat taatcgaaag attacgccta
      181 gtggttattt gataaaagaa ttcacttatg aaaaaaataa aaaaagcatc caaagttact
      241 tattgaaaga aaaagaaaac tatcctgttc aacatgctac gaaaaaaagg tggacaactg
      301 tccgttatgg taatagcgat cactatatcc attacgatga aatcctttat ctaatgagca
      361 tgccaggtta taaaaataaa ataggcctag tattgaaaga tgaagagatt ctcgtaaacg
      421 gcacaattaa aaaatataaa aaatcactcg aatatgagta tttat
//
LOCUS       DAJSMI010000381          460 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJSMI010000381 DAJSMI010000000
VERSION     DAJSMI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.391"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(20..91)
                     /locus_tag="ON430_002939"
     tRNA            complement(20..91)
                     /locus_tag="ON430_002939"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ON430_002940"
     tRNA            complement(113..200)
                     /locus_tag="ON430_002940"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ON430_002941"
     tRNA            complement(225..298)
                     /locus_tag="ON430_002941"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ON430_002942"
     tRNA            complement(312..382)
                     /locus_tag="ON430_002942"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ON430_002943"
     tRNA            complement(384..459)
                     /locus_tag="ON430_002943"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAJSMI010000382          457 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJSMI010000382 DAJSMI010000000
VERSION     DAJSMI010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.392"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            67..264
                     /locus_tag="ON430_002944"
     CDS             67..264
                     /locus_tag="ON430_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HCR3220779.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            266..>457
                     /locus_tag="ON430_002945"
     CDS             266..>457
                     /locus_tag="ON430_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR3220780.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYT"
BASE COUNT          174 a           46 c           82 g          155 t
ORIGIN      
        1 gttttttgaa atttaatgaa tgttagaata atagtctaaa aacattaatt taggggtgat
       61 tagattatga aacatttaaa aattttgtct attaaagaga cacaacttat ctatgggggt
      121 accactcata gtggaaaata ttatggaaat ggagtgtatt gcactaaaaa taaatgtacg
      181 gtcgattggg ccaaggcaac tacttgtatt gcaggaatgt ctataggtgg ttttttaggt
      241 ggagcaattc cagggaagtg ctaaaatgaa aaaaaatgct aagcaaattg ttcatgaatt
      301 atataatgat atatctataa gtaaagatcc taaatattct gatattcttg aggttttaca
      361 aaaggtatat ttaaaattag aaaaacaaaa atatgaatta gatcccagtc ctttaataaa
      421 tagattggtg aattatctat attttactgc ttatact
//
LOCUS       DAJSMI010000383          454 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJSMI010000383 DAJSMI010000000
VERSION     DAJSMI010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.393"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(116..400)
                     /locus_tag="ON430_002946"
     CDS             complement(116..400)
                     /locus_tag="ON430_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3220781.1"
                     /translation="MNQMKNEKDQPFDPDYLNRCMKTVVKENKEIREITIHGFRHTHC
                     SLLFEAGATLKEVQDRLGHANSDITLKIYTHVTTEAKRETANKLALYLDS"
BASE COUNT          123 a           68 c           74 g          189 t
ORIGIN      
        1 attgtgtttt atgtgtctta cgtttgttta atatgtatgg aaagccttta ataggtgaat
       61 agaaaattta tttttgttga actaaaaaag aagctaattc gtatgaacta gcttcttagc
      121 tatctaaata taaagcaagt ttatttgctg tttctctttt agcttcagtt gtgacgtgag
      181 tataaatctt caaagttata tcactattag catgacctag acggtcttgg acttctttca
      241 atgttgctcc tgcctcgaat aacaatgaac aatgagtgtg tctaaaaccg tggattgtga
      301 tttctcgtat ttctttattt tctttaacaa cagttttcat acaacgattc aaatagtcgg
      361 ggtcaaatgg ttggtctttt tcattcttca tttgattcac ctcttttcct tttttatttt
      421 tacgaggatg attataccat ttttggttat ctaa
//
LOCUS       DAJSMI010000384          454 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJSMI010000384 DAJSMI010000000
VERSION     DAJSMI010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.394"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..236
                     /locus_tag="ON430_002947"
     CDS             <1..236
                     /locus_tag="ON430_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220782.1"
                     /translation="VGKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMA
                     TASYIYQLMMFKQARDAYQKEFEKANKEKKLQK"
BASE COUNT          164 a           67 c           92 g          131 t
ORIGIN      
        1 ctgtgggaaa aggatccatg gatatttctg ttattcaagg tgcaaaggct gtgtatattg
       61 cttaccgcca agcgaacatg actgattata tttcattcga tgaatttatc gataaatggg
      121 attttgatat ggctactgcc agctatattt atcaattgat gatgttcaaa caagcacgcg
      181 atgcttatca aaaagaattt gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat
      241 tccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg acgttttttc
      301 tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc aaatggctta
      361 caacaagagt gcttatgaag gatggaaaaa ctatgcaata aaccaatccc agaaaaacta
      421 aagaaaggag gtaaaaaatg gctaaaaaga gaac
//
LOCUS       DAJSMI010000385          451 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJSMI010000385 DAJSMI010000000
VERSION     DAJSMI010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.395"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(131..292)
                     /locus_tag="ON430_002948"
     CDS             complement(131..292)
                     /locus_tag="ON430_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR3220783.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          156 a           89 c           54 g          152 t
ORIGIN      
        1 tcattattgt taaacaagaa tacaagcaag aaagaactat agttaatatg ataaaaatta
       61 ttaaagttct atttttcttt cttgtaatat atgctatagc attttttatc actttcctaa
      121 ctactccttt ctattttctt actccataac taattgcatc cactggacaa gcatttttac
      181 atcgtccaca tctgatacac tccagatgat tgcaattctc aactgaatct ttattcattt
      241 ggcaaacttt tttacatttt ccacaattaa tacacttatc cttatcctga atcggctaca
      301 ctagactaga cagaaaaaat aaggtgtgta gaataataaa aaacacactg gaggattttt
      361 catgtcaaga cgtcaacgaa gaacctattc aaaagaattc aaacaacaaa tcgtcgatct
      421 ctatctcgct ggtaagcctt gcgcaggaat t
//
LOCUS       DAJSMI010000386          449 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJSMI010000386 DAJSMI010000000
VERSION     DAJSMI010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.396"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            52..417
                     /locus_tag="ON430_002949"
     CDS             52..417
                     /locus_tag="ON430_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220784.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          128 a           87 c           68 g          166 t
ORIGIN      
        1 atttacaata aaactaacaa aataataaca cttgttaggt ggtgatgaaa gatgataaaa
       61 acttgctgga aaaaccttcc gttgctttta tcttttgttc cttatgtgca tttcgcctta
      121 ctgcttgatt ttcgttatca ttccgtaagc gggttcatca cattgatatt tttatctctt
      181 tttgctggat attattttca aaggaacagg cgtattatat ctttattcat tgccaatatt
      241 atatctactg tcacttccta tttattttgc gccaatttta ctgaatggcg ttacttctat
      301 catcctttaa aaccgaccca gctgatcttg cttcttgcag gtatttactt agttccacaa
      361 atccttgggt cgttatgggc ggtagcctta tcttataaaa aagctcggca tccataaaaa
      421 caaaccatat atgtacaaag agaagctac
//
LOCUS       DAJSMI010000387          448 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJSMI010000387 DAJSMI010000000
VERSION     DAJSMI010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.397"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>448
                     /locus_tag="ON430_002950"
     CDS             <1..>448
                     /locus_tag="ON430_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCR3220785.1"
                     /translation="EIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIR
                     SEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYY
                     TKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKL"
BASE COUNT          168 a           50 c           80 g          150 t
ORIGIN      
        1 gaaatatatc tgtttcttta tgaaaataaa atactggaat atgttaaaaa tggagatatt
       61 cgaaatttag aaaacatggt ttttaatctg agtaatggaa taattccaag tgttagtgga
      121 gatacgattc ggtctgaaaa aaattattct atcattgttt ttgaaaagtt agcacaaacg
      181 tctataacat taggaatgga tataatagaa gcgtatcaaa gtagagatgc tttgatacaa
      241 gaaaacgaat tagctgtatc gttacctgag gtcttaaaag tgagggattc tgggattgtt
      301 tattatacta aggaaatcgg aaaaactaag atagagcatc tttctccctt aatttcttct
      361 gtcgttcaat tcatcggctt aaatatctat aaaagaatta ctgtaaagga aatagctaat
      421 tatttttctg taagtgaaac taaattaa
//
LOCUS       DAJSMI010000388          432 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJSMI010000388 DAJSMI010000000
VERSION     DAJSMI010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.398"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
BASE COUNT          126 a           69 c           59 g          178 t
ORIGIN      
        1 ttaagtttag aatctatttt tagctcccct attaattttg aaacgtttag atcatatggc
       61 atgcaagtaa ataattttta cccatggtta actctatatc ctttattttt attgatcaag
      121 tttactaatt tagcaatagg ctataacctt tttctttata ttgttacgct cattactcta
      181 tttatatgtc attatgtaat gtatgaaatt accaaaaaac atgttactag ctcctttttc
      241 gctattatat atacaacatc ttcttttagg agtgtagaaa tatttcttcg aggggcaatg
      301 ggggaattac tcgcaatgtc aatacttcct cttattttac tcgggtttat aaagctttac
      361 gactcaaaaa aagaatcttg ggttatgttg gcgattagca tgactttatt aatttacact
      421 catgttcttt ct
//
LOCUS       DAJSMI010000389          429 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJSMI010000389 DAJSMI010000000
VERSION     DAJSMI010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.400"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
BASE COUNT          182 a           56 c           71 g          120 t
ORIGIN      
        1 gacatgtgta aatttacatg atatattcat ttctgttgtt ttaagtagca aagcaataaa
       61 atagtatttt aaaaaacata taaaatagtt gttgacatgt tagaaatgac atgataagat
      121 aactgagttg ttaaaaaaga accacttgtg aagcttgatt ctatcgaaaa aaacttcaaa
      181 aaagttcttg acaaataaca aatgataagt tatgataaat gagttgctgt aagatttcga
      241 aaaacgaaat caaaaaaact tttaaaaaag agttgacaat tacttgtctc tctgatatga
      301 tataaaagtt gctacggcag ccaaaaacga atcggggaac actcctgatg aagtagacct
      361 ttgaaaactg aacaaagtaa gacaaaccaa atgtgtaggg cgtcttgatt caattcaaga
      421 caacaaaca
//
LOCUS       DAJSMI010000390          425 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJSMI010000390 DAJSMI010000000
VERSION     DAJSMI010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.401"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            20..322
                     /locus_tag="ON430_002951"
                     /pseudo
     CDS             20..322
                     /locus_tag="ON430_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          163 a           48 c           77 g          137 t
ORIGIN      
        1 tataagaagg agtataaaaa tgaacaaaca taatagaaaa ataacgaata acaaagaact
       61 gatcttgtta ctaataggga tttggggtat ctttttactc tttaatatgt atatgccgac
      121 tatgagagcg gatgacgtag tttatgcaag tagattagat gaacttggct atttgggtgc
      181 ttcgatagaa cattataaga catggagttc aagaattatt atagaactat tcttaatgtt
      241 cttttctaaa cactttatgc tatggaaatt attaaattct acgattatgt taggaattgt
      301 agtattactt tgcaagtatg ttgagagtat aaaatatttt gtgtaaatga aaaaatccat
      361 acaaaaaagg aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag
      421 gactt
//
LOCUS       DAJSMI010000391          404 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJSMI010000391 DAJSMI010000000
VERSION     DAJSMI010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.denovo.402"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            <1..>404
                     /locus_tag="ON430_002952"
     CDS             <1..>404
                     /locus_tag="ON430_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220786.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DAJSMI010000392        10027 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.guided.092, whole genome shotgun sequence.
ACCESSION   DAJSMI010000392 DAJSMI010000000
VERSION     DAJSMI010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.guided.092"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(188..1060)
                     /locus_tag="ON430_002953"
     CDS             complement(188..1060)
                     /locus_tag="ON430_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3220787.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1188..1757
                     /locus_tag="ON430_002954"
     CDS             1188..1757
                     /locus_tag="ON430_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR3220788.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1824..2561
                     /locus_tag="ON430_002955"
     CDS             1824..2561
                     /locus_tag="ON430_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3220789.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2792..4060
                     /locus_tag="ON430_002956"
     CDS             2792..4060
                     /locus_tag="ON430_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3220790.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4547..5011
                     /locus_tag="ON430_002957"
     CDS             4547..5011
                     /locus_tag="ON430_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3220791.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4995..5624
                     /locus_tag="ON430_002958"
     CDS             4995..5624
                     /locus_tag="ON430_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3220792.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            5978..7456
                     /gene="msr(C)"
                     /locus_tag="ON430_002959"
     CDS             5978..7456
                     /gene="msr(C)"
                     /locus_tag="ON430_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR3220793.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7503..7949
                     /locus_tag="ON430_002960"
     CDS             7503..7949
                     /locus_tag="ON430_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR3220794.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8148..8663)
                     /locus_tag="ON430_002961"
     CDS             complement(8148..8663)
                     /locus_tag="ON430_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3220795.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8780..9981
                     /locus_tag="ON430_002962"
                     /pseudo
     CDS             8780..9981
                     /locus_tag="ON430_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         3194 a         1753 c         2080 g         3000 t
ORIGIN      
        1 taacgtaatc aattttatat agacagttat gttttgtcaa ttcttttttt ataattatag
       61 taatttactg gaattatcag aaataaaagg aagaaataaa ttaagaaact tatgttattt
      121 aatcgttgga tcttttgctt atcttattcg ttgttaaaaa gctgaaagcg aaggaaaaaa
      181 actctactta actgtatttc tccgttagga catctaaaaa tttacgagca gcaggagaaa
      241 gaggctggtc ttttttccaa atgagattca gtgaagcttg taatggcgga tcgagtggga
      301 tgaatatttt ttccgtccca taagtattga tgatcccatc tagagctaat gcaccaaaag
      361 tcccgttatc gaccatcaaa gaagcattgt acaataagtt atatgttccg acgatcgata
      421 actgatctat gctgaaacct agccattcag ctagttgact gtccactgac gtttgccggg
      481 aaatgatcaa aggaacagtt ttcagttcct gcggatggat gacggtttgc tttgctaaag
      541 gatgatttcg atgtatcaaa agtccccaac gatcttgggc tggcagtcga aatgactggt
      601 atttttgttt gtttgtcggt tcgattacga gtccaaaatc aagtaatccg ctatccagtt
      661 tgtctatgat atcatctgca ttcccgctat ataaatgaaa ctggatatca ggatgcgcct
      721 tgatcaacga atgaaggcta tcagaaatga aagagattgc tttagtttcg cctccgccaa
      781 tgtagatttc tccagagatg attgcgtcat ttgttaaatt gttcgccgtt ttttctacca
      841 acgacaagat ttcttttcct tttgaataaa gatatcgacc agaatccgtc aactgtatct
      901 ctcgggagcc ccgagtgaaa agtgtcacac caagttcttc ttctaaatct ttcagttgtt
      961 ttgagagagt aggttgcgtg atatgcagag attttgctgc tcggctgata tttttttctc
     1021 tagctactgc taaaaaatac gtcagtatac gaatatccat tttttccctc ctgtttttaa
     1081 aaaagcatac cactaagtat agttaaaagg aatagttaag tatgttttat aagtattaga
     1141 catgataagc cgtccgttat aaagtaagga taaaaggagg gagcaaaatg acaagcctca
     1201 tcgaaaaaca aatcgtcaat aaagagattc gagtaacgga tccgctcttt gaagtgatcc
     1261 atcagatcca agctgaaaac gaagagcctt tagcagcgct gaatactggg taccatgaac
     1321 cagcagatat ccgcaaacgg ctagaaaata tcattagtga caaagtagat gatacggtga
     1381 ctgttctgct gcctttttat acagatttcg gcaaacatat tagtataggg aaaaatgtct
     1441 ttatcaatcg tcaagtgatg ttcgttgact taggtggcat ctgtttagaa gattcagtat
     1501 tgattggtcc tcgtgtcaac ctcatcacgg tgaaccatct aaccgatcca aaagaacgaa
     1561 gaggcttgtc agtaaagcca atccacatca agaaaaatgc ttggatcgga gcaggagcaa
     1621 ctattctgcc aggcgtaacg attggcgaga atgccattgt tgcagcgaat gcaactgtta
     1681 cgaaagatgt cccagacaat accatcgttg caggaatccc tgccaaaatc gtcaaacctg
     1741 tcgaacggat tatttgaaca gtaagatgaa ataagagata gtgagtataa gcaagagatt
     1801 tttcaaaaca ggaggagaat agaatgaatg atttatcaga aaaagtgatt gtcatcatgg
     1861 gcgcttcaag cggaatcggt gaagcaacaa cgaagctatt agcagaaaaa ggggctaaac
     1921 tagttatagc tgcacgccga gaagatcgcc taaaagcaat aaaagaatct ttaccagaag
     1981 ctgagctata tattcaaaca gcagatgtaa gagattttgc acaagtccaa gcagttatcg
     2041 atttagcgat ggaaaagttc ggtcgaatcg atgtattgta caataatgca ggaatcatgc
     2101 caaccgctcc attagttgaa ggtcaccgtg atgaatggca aaatatgctt gatatcaaca
     2161 tcatgggtgt gttaaatgga atttccgccg tacttccaat catggaaaaa caaaaatctg
     2221 ggcatatcat ttcaactgat tctgtggcgg gacatgttgt ttatcctgat tccgccgttt
     2281 attgcggaac aaaatttgct gtccgtgcaa tcatggaagg attacgtcaa gagcaacggc
     2341 aaaataacat caaatctacg atcatctctc caggagctgt tcaaactgaa ttatatcaaa
     2401 ccatcagcga caaaaaggct gctcttgaac ttcatgaagc gcaaaaagaa tggggattga
     2461 cctccgaaga tatagcttct gctgtagcat ttgcgataga aacgccagac cggatgtctg
     2521 tcagtaacat gattatccgt ccgacgacac aggaagtata atccataacg gataatatcc
     2581 aaagagaatt acttgttaaa tagttattca aacgaccatc aatttaaggc gtgaatggag
     2641 aagaatattc ttcatttacg tctttttatg tagaaaaagc aaatttatca aaaagaaaca
     2701 aatgttattt aaatatggac attgacttgc ttttactttt ttagttacac tgttatttag
     2761 gaaaagtttc ataattcaaa ggagaaaaaa gatgaaagca aagaatttat tatttagcgt
     2821 tattctttta ggaaatctag cagttgccaa cacaacagtg ctagctgaag aatcgacaga
     2881 aactgtcgca actcagacat cgagttcttc agaaacagga attgaaaacg atacagagag
     2941 tactttgcag tcagctactg tctcagaaaa tacaatttca accgaagaac caacaactga
     3001 aacaacagaa caaacacaaa ccgaagacag tacatcagca gaagctcaga acaatccaga
     3061 gaatgcagaa gaaatcacga accaattgac agatgggcaa acattgacac atacccatca
     3121 agcagatttg tctgaattcg aaaactatac tggctatgct ttttatgctc gctcatctgc
     3181 taattctcaa caagcgttca ttgatagtat tgcctcaact gcacaaagtt tggcaagtgc
     3241 caatgactta tatgcatcag tcatgattgc tcaagccatc gttgaaagtg gctgggggaa
     3301 cagtactttg gcttctgccc ctaattacaa tctatttggg attaaaggaa gctataatgg
     3361 ccaatctgtt acgatgccta cttcggaata tgtgaatggg caatggatta cggtgaatgc
     3421 cgcttttaga aaatatcctt cgtacaaaga atcattgcaa gataacgtaa cggtattgaa
     3481 gacgacttct tttcagccag gcgtttacta ctattccggt gcatggaaaa gcaatacgaa
     3541 ctcttacaaa gatgctacgg cttggctaac aggtcgttat gccactgcgc ctaattatgg
     3601 ttctacattg aacaatgtaa tcgaaaccta taatctgaca cagtatgata cagcaccaac
     3661 ttcaacttct ccgatgtatc gtctgtataa ccgtcataca ggagaacatt tatatacatt
     3721 gaatgccgga gaaaaaaatt atctgccaac tgtaggctgg gaatatgaag ggattgcttg
     3781 gcaagcacca aacagcggac aacctgtcta ccgtttatac aatccttaca gtggagacca
     3841 tcattacaca atggcacaat ctgagatcaa ctctttaaca aaaatcggtt ggcgttatga
     3901 aggtctaagt ttttattctg gtggttccaa gccaatttat cgactattta atccaaatga
     3961 aaaaactggt acccatcact ataccttgag tgctaaggaa agagattggc taacaccgat
     4021 gggctggaga tatgaaggaa taggatttta tggttattaa aaagaggtag tgaaaaagct
     4081 gccctgcatc aagtagtaag aacagccaaa caaaaacaga ttttcatgtt ttttgtttgg
     4141 ttgaacttat taccgcagat gcaagttttt gaacaccgtt tactcaaaaa aggttgtgac
     4201 aacatcttgt cataactttt ttattactgt ttaagcatac tgatccaatt tctaatattc
     4261 gacgggattc ttgcaaaagg cgatttcgga aatttttggc ggtgtaaaat tttctactgc
     4321 agaagagcta tcttctatag tttgataaga atcaataatc ttggttttag cgttgtgctt
     4381 tctttatcat tttttccgtt cattctacaa aaaatttctt ttctctatac ttttctattg
     4441 agagcgtttt ttttattaca cagctgattc tttgactttt ttcttttttt attctattat
     4501 tgacaagtca atcgttgacg agtcaattat tagaaaggag gaaattatgt ctttagaaga
     4561 gattagtcac cttttatatc aaatcaaatt agccgatcaa aaaatcgtcc agatctttga
     4621 agaaaatatc ggtataagtc ttactagata cgagatcatg atgagactga aagaagaaaa
     4681 tccattgcta caaagcagtt tgcaagaaaa gttgcagatt gatcaaggag cagttactcg
     4741 tcatctgaag gtattggaag caaatggcta tgtttcgcgt cagagaaatc ctcagaacaa
     4801 ccgggaagtg tatgtagcgc taacagaaaa agcccagaag aaaatcgagc attgtgaaat
     4861 ggaccataaa gatacaggtt ccgtgctatc cccggctttc agcgatgtag aaatgaaaca
     4921 atttctaaca cttttagata aattgaatac ccaactttgc aatataacaa cagaaacgaa
     4981 agaggttaat aataatggaa caaaaaaatg atttttcaac aattatgact ggtagaagat
     5041 ccgttcgcgt atatgatgaa acatacaaaa tcccacacga agaaatgctg gaaatgatca
     5101 aagaagcgac gactgcacct tcttctgtca atatgcaacc atggcgcttt gtggtcgctg
     5161 aaagcgagga agcaaaagaa atcttgcgtc ctttgatccg tttcaatacg agacaaaatg
     5221 atacttcttc tgcaatggtc atgatttttg gcgatatgca atgttacgaa tatggcgaag
     5281 aaatctataa ccaagcttac gaatcaggga agatgccaaa agaagtcaga gatcaacaat
     5341 tagctgcgat cattccttat tacaagagtt tttcaagaga agaaatgaac gatgtagtca
     5401 aagtcgattc tagcttagcg gctatgcaat tcatgttagt tgcgcgtgat catggctatg
     5461 aaacaaatcc aattggtgga ttcgaagcag atcagttggc agaggcattt ggtttggata
     5521 aagaccgtta tgttccagtt attattttat caataggaaa agcggtagaa gaaggttacg
     5581 agtctgttcg tttagcacca gaaaagatca ctacgttcga ataaaaggtt ttcagttact
     5641 attcagtagc tattgctttt tttgaatcgt gtgtggtaac ctaataaaaa aggaggtcgg
     5701 aaaaatgact gcatcgatga aattacgttt cgaacttttg aataacaact aaataaattc
     5761 aaaagacatg tataaacaac aatttcatgg atggagtatg tccatttttt cgaacgacag
     5821 tagaataggt gaaagctatg acagatttgg tgatttagtc atagttattt cgttcagagt
     5881 aggaaaagat gtgatgcagt ttttttgcaa acagattttt ctgctctttt ttagtatcga
     5941 taattgttgt tttacatgaa ataggagtgt aaaacatatg gaaaatttag cagtaaatat
     6001 aacaaatcta caagtcagct ttggcaacca gctagaacta tctattgatt ctcttcgtgt
     6061 ctatcagcaa gatcgtatag ggatcatagg agaaaatggt gtcggtaaat cgactttgct
     6121 caaactaata gccggtgaac tttttcccga tcatgggaaa atccaaacag agatcacctt
     6181 caactacctg cctcaattaa cctatctcgc tgaagcaaag gacctaaatt tggaattagc
     6241 cagtcatttc cagttaagac tggaagaaac ttcagagcgg aaatggagcg gaggggaaga
     6301 acgaaagatc gagttgatac gtcttctttc ttcttatgaa caagggatgc ttctagacga
     6361 gccaacaacc catctagata gaaaaagtat tgatcgactg attgaagagc ttcgttatta
     6421 ttatggtacg ctggtttttg ttagtcatga tcgctatttt ctagatgagc tagcatcgaa
     6481 aatctgggaa gtaaaagacg gagaaatccg agagttttcg gggaattata gtgcctatct
     6541 cactcaaaag gaattggaga aaaagactca gctacgagaa gcagagtcga tcatgaaaga
     6601 gaaaaaacga ttggaaaaat cgatccaaga aaagaaaaaa caagcggaaa agttggaaaa
     6661 agtgtccagt aaaaagaaaa agcaacaaat cagaccggat cggttgtctt cctctaaaca
     6721 aaaagacagt gtacaaaaag ccatccaaaa gaatgcgaaa acattagaga gaagactcca
     6781 aaaaatagga gaaacaacca agccgcagca gatgaaacaa atccgttttc cagtaccaaa
     6841 atctcttgaa ctccacagcc gctatcccat catgggacaa aatgtccaat tggaaagaag
     6901 tgggagaaca ttactggtaa atggtgattt tcagttttct ttaggtaaaa aaatcgcgat
     6961 tgtcggtgaa aatggttcag gtaagacaac cttattagaa catatccgca aacaaggaga
     7021 aggaatcctt ctctctccga aagtaagctt tcaagtatat cagcaaaagg gttatcaaat
     7081 gacatctgaa gaatccatca ttcgttttgt catgagacaa acagagtttt cagaatcgct
     7141 tgtccgtagt ttgctgaatc acttagggtt tgctcaggaa actttgacga aaccgttatg
     7201 tacattaagt gggggagaag cgacccgtct gacgattgct ttgcttttta ctaagccaag
     7261 taatgtgttg ctgttagatg aaccgactaa ttttattgat atggcaacga tcgaagcttt
     7321 agagaagcta atgcaaatat atccgggaac gattttgttt acttctcatg attcttactt
     7381 tgtcgagcgt acggctgatg aagtttatga aataaaaggg cagaaaataa aaaaagtact
     7441 tacgagaaat ttttaaatgt gctacgatga ttttcgtgac aaatttaaag gagtggtaca
     7501 gattgcctac aaataaaaaa gaaggaataa tttttacaac aatgatgtgt tttttgatgg
     7561 tcttggggat gagcgcgtat aatttatggc tgcatcaaga tcttcattta tcaagtttgt
     7621 tgattggtct agtgcctgga ttcgtggtgg ctttcatttt ggatgtcttc atcatcggtg
     7681 tgctggcgaa aaaaattgcc tttaaactac cagtaaatcc agaaagcaag gtccaaatga
     7741 tcttaactat ttcatgcttg atggttttag gaatggtgac atgtatgtct ttattcggtg
     7801 tgatcatgga acaaggaata cctgatcaat tattctctgc ttatatgaat gcttggaaaa
     7861 tgaatgtgat tgccgcttta ccgttgcagt tgatcatcgt tggcccgctt tcaagaagtg
     7921 tgctaaaagc aattcaaagt aatgaatgat ggaacactgt tatttgaaaa aaggccttgg
     7981 catgcttttt gtcatagtct cttgaatgca gttctagaaa aatagtgatc cgaacgatag
     8041 gttttggcct gtcgttcgga tcgctttttc ttttccaact gtattgctct tttattgaag
     8101 ataatttgct cttcaataga tatttctaga aaggttctgt ttatccctta ctcaaaaaac
     8161 tgctgcattt gttctcttac ttgctgacac cattggagtt cctgctgaca aagcataatt
     8221 ctgcgctgga tcacaacata acgtccaaat tcttgtttct tgtctagcga gaggtcattc
     8281 aatttttttt gatttttttg catgattttg ttcagctgtt gttctctagt gaatagcagt
     8341 ccagtagctg ttgttttatc gagcgtcgaa aaagcataga tttttgctag aaattcatct
     8401 ttttgcgtag gagcgggagt ttcctctttg atccattcct ctacaagagc acgtccttca
     8461 tcagtcaagt ggtagatctt tttttgagag tgctcgtctt tgtccaaaac ggtcacataa
     8521 ccctgctggt ctaacttggc aagcgttgta tagatctgac tgtgatgggc ttcccaaaaa
     8581 attgtgatca gctgcttcaa ttcataccca gaacgtggtt tggataaaag cacgcataat
     8641 aatatatagg ctaaagtatt cataaaaatc ctacctctcg tcttattttc tttattatat
     8701 cacagtaaag tttccacgtt ttacatgtgc atatttacat gtaaaataaa tcatgtatga
     8761 tgctaataga gagaaaggga tggaaatatt ggagaatcaa atagatatac aagaacaaac
     8821 aaatgaagaa acaaagggga aaagttcagc gctagttgta gcagctacct gtatgatcgc
     8881 ttttatggga gtgggtctag tagacccaat cttaaaaacg attgctgcac agttaaacgc
     8941 tacgccagct gaaacgaccc tgttatttac tagttacatg ttagtcactg gtgtggtgat
     9001 gctatttaca ggtttcttat ctagtcggat cggtttgaaa aaacattgat gacaggattg
     9061 tttatcatcg ttgtattcgc tggtcttggc ggattatctg ggaatatcgg aatgctgatt
     9121 gggttacgtg ccggctgggg tgtcgggaat gctttatttg tctctactgc tttagcaacg
     9181 atcgtcagta ttttagttgg taacacagaa aaagcaatca tgatgtatga agcagcactt
     9241 ggctgcggaa tggctgttgg ccctctagtc ggtggggtct tgggcagtgg ttcatggcgt
     9301 tatccatttt ttggggtagc atttttgatg ttttgcggtt ttatagccct agcggtactt
     9361 ttacctgaaa ctgagaaacc aaagagaaaa gtaggccttt gggaaggtgt caaagcgtta
     9421 agcaatcata aacttcgttc agttggacta atcgcattgt tatataattt cggattcttt
     9481 actttacttg cgtatgcgcc atttttattg attggctatt ctgaattaga agtcggtttt
     9541 gtattttttg gctggggtgt gcttctggct attgcttcga ttttcgttgc accggctctt
     9601 gagcggaagt ttacgactta tcggacaatt ttagcagcat ttgtcttatt cattatttgt
     9661 ttgctgttat taggagctgg aacttctatg ccagtccttg taccgatcag cattgtgtta
     9721 gcaggatttt tccaaggaat tatcaataca ctgttgacga cgattgctat ggaaatacca
     9781 ggtctgcaac gaaacgttgc ttcgtccagc tatagttttg tccgcttttt cggtggagct
     9841 ttagcaccat ttattgcagg tgtgattggt gaacatattg gtgaaagcta tgcgttttat
     9901 tttgcaggtg ccattgttct tatcagtgta tgttgtatca tttatcaccg taattatttt
     9961 gctacaaatg aagaagaata agaaatcaga ggttgtgaaa aagctgtcct gcatcaagtc
    10021 ataagaa
//
LOCUS       DAJSMI010000393         7209 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.guided.135, whole genome shotgun sequence.
ACCESSION   DAJSMI010000393 DAJSMI010000000
VERSION     DAJSMI010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.guided.135"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            40..1008
                     /locus_tag="ON430_002963"
     CDS             40..1008
                     /locus_tag="ON430_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR3220796.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1001..2032
                     /gene="vanA"
                     /locus_tag="ON430_002964"
     CDS             1001..2032
                     /gene="vanA"
                     /locus_tag="ON430_002964"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR3220797.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2038..2646
                     /locus_tag="ON430_002965"
     CDS             2038..2646
                     /locus_tag="ON430_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR3220798.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3074..3985
                     /gene="vanY"
                     /locus_tag="ON430_002966"
     CDS             3074..3985
                     /gene="vanY"
                     /locus_tag="ON430_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HCR3220799.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4138..4623
                     /gene="vanZ"
                     /locus_tag="ON430_002967"
     CDS             4138..4623
                     /gene="vanZ"
                     /locus_tag="ON430_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HCR3220800.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(4910..5239)
                     /locus_tag="ON430_002968"
     CDS             complement(4910..5239)
                     /locus_tag="ON430_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3220801.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5544..5873
                     /locus_tag="ON430_002969"
     CDS             5544..5873
                     /locus_tag="ON430_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3220802.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            5863..6150
                     /locus_tag="ON430_002970"
     CDS             5863..6150
                     /locus_tag="ON430_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3220803.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(6429..6992)
                     /locus_tag="ON430_002971"
     CDS             complement(6429..6992)
                     /locus_tag="ON430_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3220804.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2349 a         1215 c         1458 g         2187 t
ORIGIN      
        1 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
       61 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
      121 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
      181 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
      241 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
      301 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
      361 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
      421 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
      481 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
      541 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
      601 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
      661 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
      721 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
      781 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
      841 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
      901 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
      961 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     1021 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     1081 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     1141 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     1201 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     1261 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     1321 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     1381 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     1441 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     1501 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     1561 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     1621 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagc
     1681 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     1741 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     1801 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     1861 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     1921 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     1981 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     2041 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     2101 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     2161 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     2221 tacggattgc ttctatggga cggttaccgt cctaatcgtg ctgtaaactg ttttatgcaa
     2281 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     2341 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcagtgcc
     2401 attgatctta cgctttatcg attagacacg ggtgagcttg taccaatggg gagccgattt
     2461 gattttatgg atgaacgctc tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa
     2521 aatcgcagac gtttgcgctc catcatggaa aacagtgggt ttgaagcata tagcctcgaa
     2581 tggtggcact atgtattaag agacgaacca taccccaata gctattttga tttccccgtt
     2641 aaataaactt ttaaccgttg cacggacaaa ctatataagc taactctttc ggcaggaaac
     2701 ccgacgtatg taactggttc ttagggaatt tatatatagt agatagtatt gaagatgtaa
     2761 ggcagagcga tattgcggtc attatctgcg tgcgctgcgg caagatagcc tgataataag
     2821 actgatcgca tagaggggtg gtatttcaca ccgcccattg tcaacaggca gttcagcctc
     2881 gttaaattca gcatgggtat cacttatgaa aattcatcta cattggtgat aatagtaaat
     2941 ccagtagggc gaaataattg actgtaattt acggggcaaa acggcacaat ctcaaacgag
     3001 attgtgccgt ttaaggggaa gattctagaa atatttcata cttccaacta tatagttaag
     3061 gaggagactg aaaatgaaga agttgttttt tttattgtta ttgttattct taatatactt
     3121 aggttatgac tacgttaatg aagcactgtt ttctcaggaa aaagtcgaat ttcaaaatta
     3181 tgatcaaaat cccaaagaac atttagaaaa tagtgggact tctgaaaata cccaagagaa
     3241 aacaattaca gaagaacagg tttatcaagg aaatctgcta ttaatcaata gtaaatatcc
     3301 tgttcgccaa gaaagtgtga agtcagatat cgtgaattta tctaaacatg acgaattaat
     3361 aaatggatac gggttgcttg atagtaatat ttatatgtca aaagaaatag cacaaaaatt
     3421 ttcagagatg gtcaatgatg ctgtaaaggg tggcgttagt cattttatta ttaatagtgg
     3481 ctatcgagac tttgatgagc aaagtgtgct ttaccaagaa atgggggctg agtatgcctt
     3541 accagcaggt tatagtgagc ataattcagg tttatcacta gatgtaggat caagcttgac
     3601 gaaaatggaa cgagcccctg aaggaaagtg gatagaagaa aatgcttgga aatacgggtt
     3661 cattttacgt tatccagagg acaaaacaga gttaacagga attcaatatg aactatggca
     3721 tattcgctat gttggtttac cacatagtgc gattatgaaa gaaaagaatt tcgttctcga
     3781 ggaatatatg gattacctaa aagaagaaaa aaccatttct gttagtgtaa atggggaaaa
     3841 atatgagatc ttttattatc ctgttactaa aaataccacc attcatgtgc cgactaatct
     3901 tcgttatgag atatcaggaa acaatataga cggtgtaatt gtgacagtgt ttcccggatc
     3961 aacacatact aattcaagga ggtaaggatg gcggaatgaa accaacgaaa ttaatgaaca
     4021 gcattattgt actagcactt ttggggtaac gttagctttt taatttaaaa cccacgttaa
     4081 ctaggacatt gctatactaa tgatacaact taaacaaaag aattagagga aattatattg
     4141 ggaaaaatat tatctagagg attgctagct ttatatttag tgacactaat ctggttagtg
     4201 ttattcaaat tacaatacaa tattttatca gtatttaatt atcatcaaag aagtcttaac
     4261 ttgactccat ttactgctac tgggaatttc agagagatga tagataatgt tataatcttt
     4321 attccatttg gcttgctttt gaatgtcaat tttaaagaaa tcggattttt acctaagttt
     4381 gcttttgtac tggttttaag tcttactttt gaaataattc aatttatctt cgctattgga
     4441 gcgacagaca taacagatgt aattacaaat actgttggag gctttcttgg actgaaatta
     4501 tatggtttaa gcaataagca tatgaatcaa aaaaaattag acagagttat tatttttgta
     4561 ggtatacttt tgctcgtatt attgctcgtt taccgtaccc atttaagaat aaattacgtg
     4621 taagatgtct aaatcaagca atctgatctt tcatacacat aaagatattg aatgaattgg
     4681 attagatgga aaacgggatg tggggaaact cgcccgtagg tgtgaagtga ggggaaaacc
     4741 ggtgataaag taaaaagctt acctaacact atagtaacaa agaaagccca attatcaatt
     4801 ttagtgctga ggaattggtc tctttaataa atttccttaa cgttgtaaat ccgcattttc
     4861 ctgacggtac ccccaaacta agatttcata gatagatttt ctgataactt taaaaaacac
     4921 gaatagcgga aaggatgtct tcattcgcta aagagtaata aactactttt ccatcttgac
     4981 gtgattttgc cattccatgt ttttttaaaa aacgtaaatg gtgtgacgtt gtagcgacac
     5041 tagcagtaag tatttctgct aaatcacaga cgcacatttc cttataggtt tctaaagcat
     5101 agaaaatttt aattctagaa aaatcgctga aacatttccc taacacaagt aagtttgaaa
     5161 aatctttttg atccaattta ttttttattc catttacttt ttctttatgt acgacagtca
     5221 cttggcaaac ttttttcatt catctcacct cacattcaaa cgattatttg aatgaagtat
     5281 acctatttga tcactctttt gtcaatctac cttaattcca atacactatt ataagcaatt
     5341 tcatgtattg aagtaaactt acttgcaagt gcgtttttga ttccaagtta aaaacatatt
     5401 attattctac ataatttcct aatcaaataa aactcctttg aaaattgttc taaaggagtt
     5461 ttattatttt gtatttctac taaaaatagc gtacaatgta attactaagt aattacgaaa
     5521 tgtaaaataa tggaggaaat aatatgatta caaaaacaag aaaacaagga aattctatta
     5581 tgttaacggt ccctaaagaa ttcgatgtgc ccaatggtgt tgaagtagaa gctaagcttg
     5641 tagagaatgg tatcctttat gagtttgtag agcctaaaaa agaatttttt gattttagtg
     5701 aagatatttt ggctgatatt ctttcagaag gttatagcaa acaagagatc ttaaaagaat
     5761 ttagaaatag aaaaaatgcg ttaacttctg catttaaatc tattgctgaa gatactgttg
     5821 caaattctaa accgatgaca aaagaggagt tggcagcaga aattggctta tgagattcta
     5881 ccttctaaac acgtaataaa gtatctaaaa aaattaaaag agaaaacatt aaaagaacaa
     5941 tttttaacga taatttatga tgagatagca gttcgtcctc attcgggaga acaaaaaacg
     6001 ggtgatttat caggaatttg ggctattgga ttcaagtatg caggtacgac atatcgcgtt
     6061 gcatatgaaa ttaaagataa tgcagtgata cctatcttac tatgtggaac gcatgaaaat
     6121 ttttatgaac aattaaaaaa gattaggtaa gaacccaatg tttcaacttg agaggataga
     6181 cgacacttta aaagataatt tttaacagct gagagttgta ataccctata attttaaacc
     6241 actaacctat atatttatga aaaatagaaa aaattaaaac gcattttaag cctctttcaa
     6301 attaaaagct tcatccttat gcacaaagag ggcttaaaat gcgttatttt atattgtttt
     6361 ttagtaataa taaaaaaatc actctaattc tcccataaat attatagatt cctaaagatt
     6421 aaaatagatt attccctatt gtcttttaag tagcggtaga gtgttgcctt gctaatacca
     6481 gtcatttccg taatttcttt tacgctataa acctgacttt tatacaattt tactgctcga
     6541 tcaacatctt tctggttcac tcgtggacgg ccacctttac gacctctcgc acgtgcactt
     6601 ttcaaacctt ctttggtacg ttcaacaatt aaatcacgtt cgaactgact aaatgcttgg
     6661 aagacagtca tcattaagcg tccgtgaggc gtgctggtat cgaagttttc tttcaagctc
     6721 accagcttaa catggtgttc ttcaaaatag gtaacaaggt caatcaaatc ctttgtacta
     6781 cgacccaatc gagaaaaact ttctacaatt accgtatctc ccggacgaat tttatctttt
     6841 aaacgattta gttggggacg aaccgctttg gttcccgtca ttttctcagt taggatttca
     6901 ttacaatttt gttcattgag taagtctaat tgacgattta gttcttgtga acgtgtactc
     6961 actcgggcat aaccataaat gtatccactc atgcttttct tcctttcgta ctttaattct
     7021 ggcttgattg tatcataaaa gggagttata gatacatagt taatgcaaca agttatgata
     7081 caaaatatga cctcttttat ttaataaaag aggtcatttc attttgtctc ataatcgatt
     7141 gtttttgata cgagtgtgta tacgaaataa acaaaaaaat cttaagtaaa tttctagata
     7201 cctaattta
//
LOCUS       DAJSMI010000394         5741 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.guided.159, whole genome shotgun sequence.
ACCESSION   DAJSMI010000394 DAJSMI010000000
VERSION     DAJSMI010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.guided.159"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            complement(18..233)
                     /locus_tag="ON430_002972"
     CDS             complement(18..233)
                     /locus_tag="ON430_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3220805.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQGSVAKF"
     gene            complement(776..2926)
                     /locus_tag="ON430_002973"
                     /pseudo
     CDS             complement(776..2926)
                     /locus_tag="ON430_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3072..3647
                     /locus_tag="ON430_002974"
     CDS             3072..3647
                     /locus_tag="ON430_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3220806.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3861..4556
                     /gene="vanR"
                     /locus_tag="ON430_002975"
     CDS             3861..4556
                     /gene="vanR"
                     /locus_tag="ON430_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR3220807.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4534..5688
                     /locus_tag="ON430_002976"
     CDS             4534..5688
                     /locus_tag="ON430_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR3220808.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1795 a         1103 c         1087 g         1756 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccct gttcaaaatc taaaatcaac
       61 acatcatggg gagtgtgata ataaatattt tcccttaatg taccaacaat ggtaattcgg
      121 tcaattgacc attgcatttt taagggggtg cacatagttt gtgcatttaa ccgcctgtta
      181 gaaatacggt taagtttttc ccactcattt acgattatgc cttttttact caaaaatagc
      241 tcctcctcaa cgaaaaaaga gattcttgga aagaatttcc agagttcacg taactctaaa
      301 aatccacatc caaaaatctc tagcttgaag aaacctaaaa atattcgtat aatgaacttg
      361 agttacggaa ctaggtgaag tagtttccgt aaaggtcgag agtgcgtcaa cactgctcga
      421 ctattttttt actctttttt ctcattttct ttgattatag cactcctcta gaaaaaataa
      481 aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc gaaatagaat
      541 ggcgctgcca gcatttattt cgttcagcta tcggctacac aatctaacac gaccgttccg
      601 ctaccagtcg ccttacaggc tccattttcg ctcctctaac gtgtttcacc gattgtgccg
      661 ataactaaac gaaataaacg ctaaaaccgc tcagaacgca aaatatgcca ttttagagct
      721 attgtagaaa atatagtttg aaatgcaatt ttcttctttc agctttttat ttcccacttg
      781 taaatcggta gatggtggct ccttttccag ttccataatg tggatttgca tctgcatgta
      841 gtgatgaaac acctagctgc attctcatac catctgacga agatgttgta ccgtcgcccc
      901 aatagaaagg caattgtaat ttatgatgaa agtttactaa tatggcttgg gctttattca
      961 tggcatcttc atacatgcgc cattgagata cattggctag ttgcttatat gtaagtccgg
     1021 gtgtggcttc ggccatcttg ctcaagccaa tattcattcc cattcctaaa agggcagcca
     1081 tgataatgat tgtttcttcc ttatctggtt ttcgattatt ggaagcatga gtgaattgct
     1141 catgaaatcc tgttatatgg gccacatcca tgagtaaatc agttaatttt attcttggta
     1201 gcatctgata aaggcttgca ctaaattttt ttgcttcttc tggaacatct ttttctaagc
     1261 gtgcaagtga tagctttcct ttttcaagag aaaccccatc taacttattg gaattggcag
     1321 ctaaccactt taacctttca ttaaagctgc tggttctctc cgttatataa tcttcgaatg
     1381 ataaactaac tgataatctc gtattcccct tcgattgatt ccatgtatct tccgaaaaca
     1441 aatattcctc aaaatcccta tattgtctgc tgccaacaat ggaaacatct cctgcccgaa
     1501 catgctcccg aagttctgtt aaaacagcca tttcatagta atgacgatta attgttgtac
     1561 catcatcctc gtataaatgt cttttccatc gttttgaaat aaaatccaca ggtgagtcat
     1621 caggcacttt tcgctttcca gattcgttca ttcctcggat aatctcaaca gcttgtaaaa
     1681 gtggctcatt tgcctttgta gaatgaaatt ccaatactct taatagcgtt ggcgtatatt
     1741 ttcttagtga ataaaaccgt ttttgcagta agtctaaata atcatagtcg gcaggacgtg
     1801 caagttcctg agcctcttct actgaagaga caaaggtatt ccattcaata accgattcta
     1861 aaaccttaaa aacgtctaat ttttcctctc ttgctttaat taatgcttgt ccgatgttcg
     1921 taaagtgtat aactttctca tttagctttt taccgttttg tttctggatt tcctcttgag
     1981 ccttacgacc ttttgataac aaactaagta tttgcctatc atgaatttca aacgctttat
     2041 ccgttagctc ctgagtaagt tgtaataaat agatggttaa tatcgaataa cgtttatttt
     2101 cttgaaagtc acggaatgca tacggctcgt atcttgagcc taagcgagac agctgcaaca
     2161 ggcggttacg gtgcaaatga ctaatttgca ctgtttctaa atccattcct cgtatgtatt
     2221 cgagtcgttc tattattttt agaaaagttt cgggtgaagg atgacccggt ggctctttta
     2281 accaacccaa tatcgtttta ttggattcgg atggatgctg cgaggtaata atcccttcaa
     2341 gcttttcttt ttgctcattt gttagagatt tactaaccgt attaaatagc ttcttttcag
     2401 ccattgccct tgcttcccac accattcttt caagtgtagt gatagcaggc agtataattt
     2461 tgttttttct tagaaaatct atgcattcat gcagtagatg aatggcatca ccattttcca
     2521 aagctaattg atgaaggtac ttaaatgtca ttcgatattc actcagggta aaagttacaa
     2581 agtcgtattc acttcgaatt tctttcaaat gatcccaaag tgtattttcc ctttgaggat
     2641 aatgatcaag cgaggatgga ctaacaccaa tctgtttcga tatatattgt atgaccgaat
     2701 ctgggatgct tttgatatga gtgtatggcc aaccgggata ccgaagaaca gctaattgaa
     2761 cagcaaatcc taaacggttt tcttccctcc ttcgcttatt aactatttct aaatcccgtt
     2821 tggaaaaagt gaagtaggtc cccagtatcc attcatcttc agggatttgc ataaaagcct
     2881 gtctctgttc cggtgtaagc aattctctac ctctcgcaat tttcattcag tatcattcca
     2941 tttctgtatt ttcaatttat tagttcaatt atatatcaat agagtgtact ctattgatac
     3001 aaatgtagta gactgataaa atcatagtta agagcgtctc ataagacttg tctcaaaaat
     3061 gaggtgatat tttgcggaaa atcggttata ttcgtgtcag ttcgactaac cagaatcctt
     3121 caagacaatt tcagcagttg aacgagatcg gaatggatat tatatatgaa gagaaagttt
     3181 caggagcaac aaaggatcgc gagcaacttc aaaaagtgtt agacgattta caggaagatg
     3241 acatcattta tgttacagac ttaactcgaa tcactcgtag tacacaagat ctatttgaat
     3301 taatcgataa catacgagat aaaaaggcaa gtttaaaatc actaaaagat acatggcttg
     3361 atttatcaga agataatcca tacagccaat tcttaattac tgtaatggct ggtgttaacc
     3421 aattagagcg agatcttatt cggatgagac aacgtgaagg gattgaattg gctaagaaag
     3481 aaggaaagtt taaaggtcga ttaaagaagt atcataaaaa tcacgcagga atgaattatg
     3541 cggtaaagct atataaagaa ggaaatatga ctgtaaatca aatttgtgaa attactaatg
     3601 tatctagggc ttcattatac aggaaattat cagaagtgaa taattagcca ttctgtattc
     3661 cgctaatggg caatattttt aaagaagaaa aggaaactat aaaatattaa cagcctccta
     3721 gcgatgccga aaagcccttt gataaaaaaa gaatcatcat cttaagaaat tcttagtcat
     3781 ttattatgta aatgcttata aattcggccc tataatctga taaattatta agggcaaact
     3841 tatgtgaaag ggtgataact atgagcgata aaatacttat tgtggatgat gaacatgaaa
     3901 ttgccgattt ggttgaatta tacttaaaaa acgagaatta tacggttttc aaatactata
     3961 ccgccaaaga agcattggaa tgtatagaca agtctgagat tgaccttgcc atattggaca
     4021 tcatgcttcc cggcacaagc ggccttacta tctgtcaaaa aataagggac aagcacacct
     4081 atccgattat catgctgacc gggaaagata cagaggtaga taaaattaca gggttaacaa
     4141 tcggcgcgga tgattatata acgaagccct ttcgcccact ggagttaatt gctcgggtaa
     4201 aggcccagtt gcgccgatac aaaaaattca gtggagtaaa ggagcagaac gaaaatgtta
     4261 tcgtccactc cggccttgtc attaatgtta acacccatga gtgttatctg aacgagaagc
     4321 agttatccct tactcccacc gagttttcaa tactgcgaat cctctgtgaa aacaagggga
     4381 atgtggttag ctccgagctg ctatttcatg agatatgggg cgacgaatat ttcagcaaga
     4441 gcaacaacac catcaccgtg catatccggc atttgcgcga aaaaatgaac gacaccattg
     4501 ataatccgaa atatataaaa acggtatggg gggttggtta taaaattgaa aaataaaaaa
     4561 aacgactatt ccaaactaga acgaaaactt tacatgtata tcgttgcaat tgttgtggta
     4621 gcaattgtat tcgtgttgta tattcgttca atgatccgag ggaaacttgg ggattggatc
     4681 ttaagtattt tggaaaacaa atatgactta aatcacctgg acgcgatgaa attatatcaa
     4741 tattccatac ggaacaatat agatatcttt atttatgtgg cgattgtcat tagtattctt
     4801 attctatgtc gcgtcatgct ttcaaaattc gcaaaatact ttgacgagat aaataccggc
     4861 attgatgtac ttattcagaa cgaagataaa caaattgagc tttctgcgga aatggatgtt
     4921 atggaacaaa agctcaacac attaaaacgg actctggaaa agcgagagca ggatgcaaag
     4981 ctggccgaac aaagaaaaaa tgacgttgtt atgtacttgg cgcacgatat taaaacgccc
     5041 cttacatcca ttatcggtta tttgagcctg cttgacgagg ctccagacat gccggtagat
     5101 caaaaggcaa agtatgtgca tatcacgttg gacaaagcgt atcgactcga acagctaatc
     5161 gacgagtttt ttgagattac acggtataac ctacaaacga taacgctaac aaaaacgcac
     5221 atagacctat actatatgct ggtgcagatg accgatgaat tttatcctca gctttccgca
     5281 catggaaaac aggcggttat tcacgccccc gaggatctga ccgtgtccgg cgaccctgat
     5341 aaactcgcga gagtctttaa caacattttg aaaaacgccg ctgcatacag tgaggataac
     5401 agcatcattg acattaccgc gggcctctcc ggggatgtgg tgtcaatcga attcaagaac
     5461 actggaagca tcccaaaaga taagctagct gccatatttg aaaagttcta taggctggac
     5521 aatgctcgtt cttccgatac gggtggcgcg ggacttggat tggcgattgc aaaagaaatt
     5581 attgttcagc atggagggca gatttacgcg gaaagcaatg ataactatac gacgtttagg
     5641 gtagagcttc cagcgatgcc agacttggtt gataaaagga ggtcctaaga gatgtatata
     5701 attttggctc tttgtcaaat gatgttggta aagagaaatc t
//
LOCUS       DAJSMI010000395         5115 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.guided.169, whole genome shotgun sequence.
ACCESSION   DAJSMI010000395 DAJSMI010000000
VERSION     DAJSMI010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.guided.169"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            148..1017
                     /locus_tag="ON430_002977"
     CDS             148..1017
                     /locus_tag="ON430_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCR3220809.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            998..1732
                     /locus_tag="ON430_002978"
     CDS             998..1732
                     /locus_tag="ON430_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3220810.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1765..2673
                     /locus_tag="ON430_002979"
     CDS             1765..2673
                     /locus_tag="ON430_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HCR3220811.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2670..3212
                     /gene="sat4"
                     /locus_tag="ON430_002980"
     CDS             2670..3212
                     /gene="sat4"
                     /locus_tag="ON430_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HCR3220812.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3305..4099
                     /locus_tag="ON430_002981"
                     /note="aph(3')-IIIa"
     CDS             3305..4099
                     /locus_tag="ON430_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCR3220813.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            4375..4947
                     /locus_tag="ON430_002982"
     CDS             4375..4947
                     /locus_tag="ON430_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR3220814.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         1760 a          758 c         1156 g         1441 t
ORIGIN      
        1 tcatctaatc agcagaggta taaaaagaca attgataagt tttaactatc ttttgtgaaa
       61 ttaaaaaaat tataaatatg ttgagtagaa gcaaaagatt tggcttggaa tttttacagc
      121 agtgaatcga taaaaggagc atgcttgatg gtagataata ttattaaatc agtagcagag
      181 aaattatcct ctctgtctta tatagaaggt attgttttag gtggttcacg tgcaaggggc
      241 acccatacag aggattcgga tatagatatc ggcatctatt acaattcaga atcatttgac
      301 ataaatacta ttaatcaatt cgctacaaag ctggatgatg agcatagaaa taaccttgtt
      361 gtacctcccg gagcatgggg tgattggatt aatggcggcg gatggttagt cataaacggg
      421 tatcatgtgg atttaatttt acgtgatatt aaacgtgtgg aacaaataat gaaagataca
      481 gagcacggaa ttgttactgc caattatcag actgggcatc cccatggtta tattagtgca
      541 atgtatcgag gagaattagc gattagcaaa atactatatg ctaagaatga aagcttatgc
      601 gaattaaaaa aacaggcaga aacttatccc aatgctttgc agaaaagttt agttaacttt
      661 tttatgtttg aagcagggtt ctctttaatg tttgtaaaag caaattcggg aacagacgat
      721 aaatattata ttgcgggtca tgtttttcgt atagtttcat gtttaaatca agtgttattt
      781 gcatgtaata atgcttattg tatcaacgaa aagaaagcta taaaactgct tgaaactttt
      841 gaacataaac ctgaaaaata taccgagaag gtaaatcata tttttgaagt actcggtatc
      901 tcactttttg aatgctacga catgaccgag aagctttata atgaagtgaa tgaaattgta
      961 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1021 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1081 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1141 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1201 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1261 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1321 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1381 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1441 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1501 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1561 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     1621 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     1681 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     1741 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     1801 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     1861 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     1921 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     1981 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2041 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2101 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2161 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2221 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2281 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2341 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2401 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2461 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2521 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     2581 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     2641 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     2701 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     2761 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     2821 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     2881 ataagataat atatctttac taccaagacg ataaatgcgt cggaaaagtt aaactgcgaa
     2941 aaaattggaa ccggtacgct tatatagaag atatcgccgt atgtaaggat ttcagggggc
     3001 aaggcatagg cagcgcgctt atcaatatat ctatagaatg ggcaaagcat aaaaacttgc
     3061 atggactaat gcttgaaacc caggacaata accttatagc ttgtaaattc tatcataatt
     3121 gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     3181 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     3241 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
     3301 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
     3361 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
     3421 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
     3481 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
     3541 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
     3601 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
     3661 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
     3721 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
     3781 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
     3841 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     3901 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     3961 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     4021 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     4081 actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa ctacaagctt
     4141 tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg atgttttata
     4201 tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag ttttggtgaa
     4261 gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa cagcagctag
     4321 cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata aaatttgaac
     4381 aggattaaca gagtaacctc tattcttatt cagctgcaat caaaaaaaat aattcctgcc
     4441 aaagaaattg cacagcgatt taatataagc ttaaggacag tttacagaga tatccggaca
     4501 cttgaagaag ccggaatacc aattggatct gaggccggaa aaggatattt tcttgtagag
     4561 ggcttcctac ttccgccggt aatgtttaca gcggcggaag tgggtgcatt gattacagca
     4621 gggaaatttt taaattgcca tggagatgaa tcatttataa aggattttga ttcagctatg
     4681 tataaaatca aatctatttt gaagcatggt gaaaaaaact atgcacagga gttggagaac
     4741 agtattaacg tgtacagcac atctggacag aaaaatacat tggcggataa cgttattgca
     4801 gcaattcaga ctgcaatctg caataaaagg gtaatatcaa ttcaatatcc tgcatcagga
     4861 gggcaagaac cggaaagcag aatgatagag cctatatcac tggggtttta tgaacagaac
     4921 tggtacttaa tcggttttgc aggctaagaa atgaatatag gaattttcgt gtggatcgga
     4981 ttaaaagcat ttgcatagaa gaagagctct gtcaaagcca tgatggctct ttggaacaaa
     5041 tattaaaaca aatgctttca tataaaaaac tatatgccct acaatgccgt tgtaaaaatt
     5101 ggggaaaagg gatat
//
LOCUS       DAJSMI010000396         2329 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.guided.236, whole genome shotgun sequence.
ACCESSION   DAJSMI010000396 DAJSMI010000000
VERSION     DAJSMI010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.guided.236"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            135..1637
                     /gene="eat(A)"
                     /locus_tag="ON430_002983"
     CDS             135..1637
                     /gene="eat(A)"
                     /locus_tag="ON430_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR3220815.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1665..2231
                     /locus_tag="ON430_002984"
     CDS             1665..2231
                     /locus_tag="ON430_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3220816.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT          797 a          412 c          503 g          617 t
ORIGIN      
        1 ttctgattca tcagtcccat aaattataga gccgtttttt cgatgtatgg gtctttttgt
       61 gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta
      121 cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc
      181 aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac
      241 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac
      301 cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag
      361 ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa
      421 tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca
      481 cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc
      541 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag
      601 cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta
      661 tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag
      721 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa
      781 atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat
      841 ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat
      901 ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca
      961 aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata
     1021 tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa
     1081 gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa
     1141 tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta
     1201 tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatac cagccaaaac
     1261 atctgagcat tagttatgca agccaaaatt atgaagacaa tcgaggaacg ttggcggaat
     1321 ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga
     1381 tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag
     1441 tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga
     1501 attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg
     1561 ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact gagattattt
     1621 ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaact
     1681 ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga
     1741 acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa ggacagatgc
     1801 aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
     1861 attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt ctcagcttgc
     1921 cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat ggactgccaa
     1981 gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg
     2041 agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc atcgaagacg
     2101 aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc aatgcttatt
     2161 ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca gaattggttt
     2221 cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca ctgcttttgc
     2281 ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaact
//
LOCUS       DAJSMI010000397         1360 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.guided.295, whole genome shotgun sequence.
ACCESSION   DAJSMI010000397 DAJSMI010000000
VERSION     DAJSMI010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.guided.295"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            1..115
                     /locus_tag="ON430_002985"
                     /pseudo
     CDS             1..115
                     /locus_tag="ON430_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            164..247
                     /locus_tag="ON430_002986"
     CDS             164..247
                     /locus_tag="ON430_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HCR3220817.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            372..1109
                     /gene="erm(B)"
                     /locus_tag="ON430_002987"
     CDS             372..1109
                     /gene="erm(B)"
                     /locus_tag="ON430_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR3220818.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1255..>1360
                     /locus_tag="ON430_002988"
     CDS             1255..>1360
                     /locus_tag="ON430_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR3220819.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPEC"
BASE COUNT          508 a          215 c          233 g          404 t
ORIGIN      
        1 aaacagcaaa gaatggcgga aacgtaaaag aagttatgga aataagactt agaagcaaac
       61 ttaagagtgt gttgatagtg cagtatctta aaattttgtg tataatagga attgaagtta
      121 aattagatgc taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat
      181 gcgtaatgta gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga
      241 taaataaata cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat
      301 aactaaaatt acaaacaaat cgtttaactt ctgtatttat ttacagatgt aatcacttca
      361 ggagtgatta catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag
      421 tactcaacca aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg
      481 gaacaggtaa agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta
      541 ttgaattaga cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aacattcgtg
      601 tcactttaat tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa
      661 ttgttgggag tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa
      721 gccatgcgtc tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata
      781 ttcaccgaac actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc
      841 tgccagcgga atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc
      901 gccataccac agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat
      961 gggtcaatcg agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac
     1021 acgccaaagt aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata
     1081 gttatctatt atttaacggg aggaaataat tctatgagtc gcttttttaa atttggaaag
     1141 ttacacgtta ctaaagggaa tggagataaa ttattagata tactactgac agcttccaag
     1201 aagctaaaga ggtccctagc gcctacgggg aatttgtatc gataaggggt acaaattccc
     1261 actaagcgct cgggacccct tgtaggaaaa tgtcctaagt gtggcaacaa tattgtatta
     1321 aaaaaatcgt tttatggttg ttcaaattat cctgaatgta
//
LOCUS       DAJSMI010000398         1261 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.guided.306, whole genome shotgun sequence.
ACCESSION   DAJSMI010000398 DAJSMI010000000
VERSION     DAJSMI010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.guided.306"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            107..841
                     /locus_tag="ON430_002989"
     CDS             107..841
                     /locus_tag="ON430_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HCR3220820.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          468 a          193 c          192 g          408 t
ORIGIN      
        1 tgatgaggtt gaacttttac acaagattgt ggacttgact aaaataagtg gttataatgg
       61 attgttaata taaacattca ttataacctc ataggagtgg gttataatga acaaaaaaaa
      121 tataaaagat agtcaaaact ttattacttc aaagcatcat ataaatgaaa ttttgagaaa
      181 tgtacattta aatacaaatg ataatattat tgagattggt tcagggaaag gtcatttcac
      241 gtttgaatta gctaaaaggt gtaattatgt aaccgccatt gaaatagatc ctaaattatg
      301 taggataact aaaaacaaac ttattgaata tgagaacttc caggttatca ataaagatat
      361 tttacaattt aagtttccta aaaataagtc atataagata tatggaaata taccctacaa
      421 tataagtaca gatataattc gaaaaattgt ttttgaaagc acagctacag aaagttattt
      481 aatagtggaa tatggatttg ctaaaaggtt gctaaataca aatcgttcac tagcactatt
      541 tttaatgaca gaagttgata tatccatatt aagtaaaatc cctagagaat actttcatcc
      601 aaaacctaga gttaatagct cgttaattgt attaaaaaga cacccttcaa aaatatcact
      661 caaagataga aaacaatatg aaaattttgt tatgaaatgg gttaacaaag aatacataaa
      721 actattttcc aaaaaccaat tttatcaagc cttaaaatat gcaagaattg acgatttaaa
      781 caatattagc tttgaacaat tcttgtctct tttcaatagc tataaattat ttaatagata
      841 agttaagggg tgcataaact gcatccctta acttatctta ttttgcttaa acaatcttgg
      901 cccgtaaact gcataactca tatgtaaatc gctccttttt aggtggcaca aatgtgaggc
      961 attttcacta tttccggcaa ttacttccat gtaaagtata acccactata ctttacttcg
     1021 taaaggtggg ctcgccgagg ggtcttgcag acggctctgt cggctctttt gagccgagta
     1081 tcagcgcacc cttatggtgc aattagctga tacaaaaaac aaagagttgg ctcatgtaat
     1141 ccaactctta aaacacaaaa aaataacccc cttggctttt cagcaggggg catatttttt
     1201 atttaattcc tcactcttta tggttctgtt gcaaagtttt ccaaaaaatc tattttagtg
     1261 t
//
LOCUS       DAJSMI010000399          806 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.guided.339, whole genome shotgun sequence.
ACCESSION   DAJSMI010000399 DAJSMI010000000
VERSION     DAJSMI010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.guided.339"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            13..525
                     /gene="aac(6')-I"
                     /locus_tag="ON430_002990"
                     /pseudo
     CDS             13..525
                     /gene="aac(6')-I"
                     /locus_tag="ON430_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ie family aminoglycoside
                     N-acetyltransferase"
     gene            556..>806
                     /locus_tag="ON430_002991"
     CDS             556..>806
                     /locus_tag="ON430_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /protein_id="HCR3220821.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSIL"
BASE COUNT          338 a           64 c          137 g          267 t
ORIGIN      
        1 gttgaaaatg aaatatgtat aagaacttta atagatgatg attttccttt gatgttaaaa
       61 tggttaactg atgaaagagt attagaattt tatggtggta gagataaaaa atatacatta
      121 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
      181 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      241 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      301 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      361 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      421 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      481 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      541 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      601 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      661 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      721 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      781 tatattagtg atgaattatc tatact
//
LOCUS       DAJSMI010000400          752 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052061
            SAMN29549225-rid15779983.guided.348, whole genome shotgun sequence.
ACCESSION   DAJSMI010000400 DAJSMI010000000
VERSION     DAJSMI010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549225
            Sequence Read Archive: SRR20032957
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474403.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 24 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549225-rid15779983.guided.348"
                     /strain="AUSMDU00052061"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2005"
     gene            139..687
                     /gene="aac(6')"
                     /locus_tag="ON430_002992"
     CDS             139..687
                     /gene="aac(6')"
                     /locus_tag="ON430_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR3220822.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          275 a          110 c          167 g          200 t
ORIGIN      
        1 gtataatttt tttattttat aagtaagata aataatgaaa gaatagagat actgattggt
       61 agctaaagat ttttgagcga gaacatttcg ttttttcaaa aggactgtta gaataagcaa
      121 gaacaagggg gaagacgtat gataatcagt gaatttgacc gtaataatcc agtattgaaa
      181 gatcagcttt ctgatttact gagactgact tggccggaag aatatggaga cagctcggca
      241 gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt agaccaagat
      301 gagttagtag gatttattgg tgcaatccct caatacggta tcacaggttg ggaattgcat
      361 ccattagttg tagaaagctc ccgacgaaag aaccaaatag gtactcgatt agtcaattac
      421 ttagaaaaag aagtagcttc cagaggagga atcacgattt atttaggtac ggatgattta
      481 gaccatggaa caacgttaag tcaaacggac ctgtatgagc atacatttga taaagtggct
      541 tccatccaga accttcgtga acatccgtat gaattctatg aaaaattagg ttataaaatc
      601 gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg atatttggat ggcaaaaacg
      661 attattcctc gaccaaattc tcaataataa aaagaggttg tgacagaagt aatcagcttc
      721 aagaaataag aaggaattta cgaaaattac tt
//
